LOCUS       DASSNH010000001         8475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_739114, whole genome shotgun sequence.
ACCESSION   DASSNH010000001 DASSNH010000000
VERSION     DASSNH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739114"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFK00_00005"
     CDS             <1..199
                     /locus_tag="VFK00_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET7591371.1"
                     /translation="ATVEVASPDDVHFQSIIVSDAFSGKPTLARHRMVYAALGERMGG
                     EVHALSLRTMTPDEAAQSGNS"
     gene            199..1458
                     /gene="murA"
                     /locus_tag="VFK00_00010"
     CDS             199..1458
                     /gene="murA"
                     /locus_tag="VFK00_00010"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7591372.1"
                     /translation="MASIVISGGKPLNGDVWISGAKNAVLPILAACLLADEPVTVGNV
                     PHLHDVTTTMELLGQMGAELVVDEHMRIRVDPRPTSRFFAPYELVKTMRASIVVLGPL
                     IAKYGQAEVSLPGGCAIGSRPVDQHIKGLRTLGADISVENGYIKARAKRLKGARVAMD
                     MVTVTGTENIMMAAVLAEGTTVIENAAEEPEVCDLAECLNCMGARISGIGTTRLVIDG
                     VERLHGTEYNVLPDRIETGTFLVAGAITGGRVRAKHTRPDVLKSVLSKLEDAGAHINT
                     EEDAIEIDMRGNRPRAVDVSTAPYPAFPTDMQAQFTALNCVAEGTGVITENVFENRFM
                     HVQELHRLGADIKLEGNTAVVRGVPKMSGAQLMATDLRASASLVLAGLVAEGETVVDR
                     IYHIDRGYENIEEKLGGLGAQIRRLPT"
     gene            complement(1597..2304)
                     /locus_tag="VFK00_00015"
     CDS             complement(1597..2304)
                     /locus_tag="VFK00_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET7591373.1"
                     /translation="MKIFRFAARAVAALLIGAQVAAAAAPPVPAFTAEYQLLRDGSPI
                     GTATLTLSRDSGDEWTFTTVSKGTAGLASLLGAGTRETSVFRWVDDRPQCESYDYTLT
                     TALKQQHRSVRCDWSRHAIEVDDKGRHSFATQPGVVERHTVPLALAAELAAGKTEFSL
                     PVAVRDRVEMQQYTAHGEQAVTVPAGTFDATRVSRGDGGEGFEAWFAPAKLPVPVKID
                     QTGKDGFSLELESWSAH"
     gene            complement(2365..3030)
                     /gene="purN"
                     /locus_tag="VFK00_00020"
     CDS             complement(2365..3030)
                     /gene="purN"
                     /locus_tag="VFK00_00020"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7591374.1"
                     /translation="MKPLAIAVLASGRGSNLAALLEARASGRLPIDVVLVAGDKPGAP
                     ALENARAHGIQAIAIDPKGFPDRPAFDRALFEQVTASGAQLVVLAGYMRIIDATVAAE
                     WTGRIINIHPSLLPKYRGLHTHRRALAAGDTEHGASVHFVTADLDGGPAIAQARIEVR
                     ADDTEESLAARLLPREHQLLVACVEAIARGRVRECDGRVWFEGSPITAPLRLDPGGHL
                     AAA"
     gene            complement(3148..4221)
                     /gene="purM"
                     /locus_tag="VFK00_00025"
     CDS             complement(3148..4221)
                     /gene="purM"
                     /locus_tag="VFK00_00025"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7591375.1"
                     /translation="MTSSNDALSYRAAGVDIDAGNAVVERIKPLVARTSRAEVLGGLG
                     GFGALFDLSKKYREPVLVSGTDGVGTKLKLAQQLNRHDTIGIDLVGMCVNDVLVQGAE
                     PLFFLDYFATGKLDVDTTVAVVGGIARGCELAGCALVGGETAEMPDMYPPGEYDLAGF
                     CVAGVEKSALVDGSAIRAGDVILGVASSGPHSNGYSLIRKIVERAGSPFDLELDGVTL
                     ADALMAPTTIYVKPILQLLHASGSPVPGPRSPVPLVHGMAHVTGGGLTENIIRVVPEG
                     LGLAIDPSTWKMPAVFDWLQREGKVPREEMWRTFNCGIGFTLILPPNDADAAAAMLAG
                     HGLRSHVIGEVIVATREHRVRIA"
     gene            4263..5003
                     /locus_tag="VFK00_00030"
     CDS             4263..5003
                     /locus_tag="VFK00_00030"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HET7591376.1"
                     /translation="MIDAGAAIGQTGVWLIGEYLAMTRFLFSLLLVFGAAAAAPAQTT
                     PDTAVPSAVPANPYSTTVPVAGTSDAQRDAAISAALANVLQRVSPGFAAPPEVLAQAS
                     GYVRAYHYRRAASGGLELQVDFDPGAVGRMVAQAANAAGGALAGQPGVAGSAGAVAAA
                     PAGSATVWVEGIENDHALATLLSTLRNDPSMSDVVPVGAKGDGVLLQLTSDQPLATAL
                     AAVAGPTGHLAPVAQPHPGADATFRWVP"
     gene            5017..5616
                     /locus_tag="VFK00_00035"
     CDS             5017..5616
                     /locus_tag="VFK00_00035"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7591377.1"
                     /translation="MSGTRATDDGSPDGPLRRLPNAVSCLRIALVAPLIVTILAGRHE
                     IALVIAGVAGVSDAVDGYLARRFRWQSRLGSILDPAADKLMLVGCLVALGWMQEAPRW
                     LVALVVARDAVIALGALAWHRILHTFEARPSWLSKATTVAQIGYVLLVLADRGFAWQV
                     PMAIPVWIVAGLTAASGLDYVVRWGLLARRELNAGKHST"
     gene            5613..6767
                     /locus_tag="VFK00_00040"
     CDS             5613..6767
                     /locus_tag="VFK00_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7591378.1"
                     /translation="MTNAQRWQWLIIAAVVGWLVWLLSPILMPFVLAGVFAYLGDPLV
                     DRLERLRLGRGLAASLVFLLMLLIVAFALVLLVPFIQRQVANFLAALPGYGAWINNHA
                     IPWLEEKLHIPASEFDVNTMIARLRGNAGTVGGVVTAVLAYATRSGIALIGVGVAVVL
                     VPVVTFYLLRDWDKLVAHVDELLPREAQPTIRRLARETDSVLGAFVRGQLLVMVGLAV
                     YYAVALRLVGLEVGPLIGMIAGLVSFVPYLGFIIGILASIVAALVQFHDGYHVVLVLV
                     VFGIGNLLESYVLVPKLVGDRIGLHPVAVIFAVLAFGELFGFIGILLALPMASIAVVL
                     LRFLRERYEASALYAGRGPPKIVGSQGPPGPGPGAGTDGLANPRGPSGET"
     gene            6764..7456
                     /gene="hda"
                     /locus_tag="VFK00_00045"
     CDS             6764..7456
                     /gene="hda"
                     /locus_tag="VFK00_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HET7591379.1"
                     /translation="MIRQFPLDLRWPPHQRLDVFHAAGNAGAVREVEALAIQPSHAWV
                     FLHGASGTGKSHLLIGACRAAAEAGREARYVSLASLPGSRAEAIAGIDGEGLVAIDDV
                     HSLAGDRDAERALFDLFNRAAAASARMLFAANASPAELGIGLADLVSRLSSCTQSALH
                     PLDDGGRRAMLRLLAGRMGLRLDDEVLDWWFARQPRDPASLVALLRRIDRAALAAQRR
                     VTIPFLRELLRE"
     gene            complement(7559..8275)
                     /locus_tag="VFK00_00050"
     CDS             complement(7559..8275)
                     /locus_tag="VFK00_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7591380.1"
                     /translation="MKALVFAAGRGERMRPLSDTTPKPLLAAAGKRLIEWHLERLAAA
                     GVHDVVINIAHLAAQFPEALGDGARYGLRIEYSREGDVPLETGGGMLHALPLLGKAPF
                     IAVNGDIFTDFDFASLPREPQGLAELVLVDNPPQHPRGDFGIDATGTLLPHADSPGAA
                     RALTFAGIGIYRAEVLGDWRGIIGDTPGAQVTPPRFSIVPLLRAAIARGEVGWRHHAG
                     AWTDIGTPARLAELNADLPS"
BASE COUNT         1266 a         2802 c         3011 g         1396 t
ORIGIN      
        1 cgccactgtc gaggtggcaa gccccgacga cgtccatttc cagtcgatca tcgtatcgga
       61 tgcattctcc ggcaagccta ccctcgcccg gcaccggatg gtgtacgcgg cgctcggcga
      121 gcggatgggc ggcgaggtgc atgcgctgtc gctgcgcacc atgacccccg acgaagcggc
      181 gcaaagcggg aattcctgat ggccagcatc gtgatctccg ggggcaagcc gctcaacggc
      241 gacgtgtgga tttccggcgc caagaacgcg gtgctgccga tcctggcggc ctgcctgctg
      301 gccgacgaac cggtgaccgt gggcaacgtc ccgcacctcc acgacgtcac aaccacgatg
      361 gaactgctcg gccagatggg cgccgagctg gtggtcgacg agcacatgcg gatacgggtc
      421 gatccgcgcc ccacaagccg gttcttcgcc ccctacgaac tcgtcaagac catgcgcgcg
      481 tcgatcgtgg tgctgggccc gctgatcgcg aaatacgggc aggccgaggt ctcgctgccc
      541 ggcggctgcg cgatcggttc gcggccggtc gaccagcaca tcaagggact gcgcaccctc
      601 ggcgccgaca tcagcgtcga gaacggctac atcaaggcgc gtgcgaaacg cctgaagggc
      661 gcgcgcgtgg cgatggacat ggtcaccgtg accggcaccg agaacatcat gatggcggcg
      721 gtgctggccg agggcaccac cgtgatcgag aacgccgccg aggagccgga ggtctgcgac
      781 ctcgccgagt gcctcaactg catgggtgcc cggatcagcg gcatcggtac cacgcggctg
      841 gtgatcgatg gcgtcgaacg gctgcacggc accgaataca acgtgttgcc ggatcgcatc
      901 gagaccggca ccttcctcgt cgccggggcg atcacgggcg gccgggtccg cgcgaagcac
      961 acgcgacccg atgtcctgaa gtcggtgctg tcgaagctgg aagacgccgg cgcgcacatc
     1021 aacaccgaag aggacgcgat cgagatcgac atgcgcggca accgtccgcg cgcagtcgat
     1081 gtcagcaccg cgccgtaccc ggcgtttccg accgacatgc aggcgcagtt cacggcgctg
     1141 aactgcgtcg cggaaggcac cggcgtcatc accgagaacg tcttcgagaa ccgcttcatg
     1201 cacgtgcagg aactgcatcg gctcggcgcg gacatcaagc tcgagggcaa caccgcggtg
     1261 gtgcgcggcg tgccgaagat gtccggggcg caactgatgg cgaccgacct gcgcgcgtcg
     1321 gcgtcgctgg tgctggcggg actggtggcc gaaggcgaaa ccgtggtcga ccgcatctac
     1381 cacatcgacc gcggctacga gaacatcgaa gagaaactcg gcggcttggg cgcccagatc
     1441 cggcggctgc cgacctgacg cacgctttcc cggtcatgcc attggccagc ggccacaccg
     1501 tcgaccgtgc tttcgctcgt caccccggcg caggccggga tccagtgctt tcccgcttct
     1561 gcgcccgaca cgtttctcgc aaagcgacga ggcccgtcaa tgcgccgacc agctctccag
     1621 ctccagcgag aagccgtcct tgccggtctg gtcgattttc accggaaccg gcagcttcgc
     1681 cggggcgaac cacgcctcga aaccctcgcc cccgtcgccg cggctgaccc gcgtcgcatc
     1741 gaaggtaccg gcaggcaccg tgacggcttg ttcgccgtgc gccgtgtact gctgcatctc
     1801 gacgcgatca cgcacggcga caggcaggct gaattcggtt ttcccggcgg ccagctccgc
     1861 cgcgagggcc agcggcacgg tgtggcgttc gaccacgccc ggctgggtcg cgaacgaatg
     1921 cctgcccttg tcatcgactt cgatggcgtg gcggctccag tcgcaacgca cgctgcggtg
     1981 ctgctgtttc agcgcggtgg tcaacgtgta gtcatagctt tcgcactggg gccggtcatc
     2041 gacccagcga aacaccgagg tttcgcgagt cccggcgccg agtagcgacg ccagtcccgc
     2101 cgtgcccttg ctgaccgtgg tgaacgtcca ttcatcgccg ctgtcccggc tcagcgtcag
     2161 cgtcgcggtt ccgatcgggg atccatcccg gagcaactgg tactcggcgg tgaaagccgg
     2221 caccggcggt gcagcggccg cggccacctg ggcgccgatc agcagggcag cgaccgcccg
     2281 ggcggcaaat cggaaaatct tcatgggtgg cgtacgcggg aaatcattgc ggcgcagctt
     2341 gccgcggccc ggctgaatcc agcctcacgc ggcggccagg tgcccgcctg gatcgagccg
     2401 gagtggcgcc gtgatcggcg agccctcgaa ccacacgcgt ccatcgcatt cgcgcacccg
     2461 cccgcgcgcg atcgcttcca cgcacgcgac cagcagttgg tgctcgcgcg gcagcaggcg
     2521 tgccgcgagc gattcctcgg tgtcgtcggc acgcacctct atgcgtgcct gggcaatcgc
     2581 cggcccacca tcgagatcgg cagtcacgaa atgcacgctg gcgccatgtt cggtgtcgcc
     2641 cgccgcgagt gcgcgacgat gggtgtgcag gccccggtac ttcggaagca acgaagggtg
     2701 gatgttgatg atgcgccctg tccattcggc ggcgacggtc gcgtcgatga tgcgcatgta
     2761 accggccagc acgaccagtt gggcgccgct cgcggtcact tgttcgaaca gcgcgcggtc
     2821 gaatgcgggc cggtccggga agcccttggg gtcgatcgcg atcgcctgga tgccgtgcgc
     2881 ccgtgcattt tccagtgcag gcgcgccggg cttgtcgccc gcgaccagca cgacgtcgat
     2941 aggcagcctg cccgaggcgc gcgcctcgag cagcgcggcg agattgctgc cgcggccgga
     3001 ggcgaggacg gcgatggcga ggggtttcat ggcagcaagc aaggatgcgc cgtcattccg
     3061 gcgaaggccg ggatccagag gttttgcaat ccatggcgct gggtctcggc cattccctgg
     3121 ccgggatgac agtggtactc cggttcgtca tgcaatccgt acccggtgct cgcgagtggc
     3181 gacgatcact tcgccgatga catgtgaacg cagcccatgg cccgccagca tcgcggcggc
     3241 cgcgtccgcg tcattgggcg gcaggatcag cgtaaagccg atgccgcagt tgaaggtgcg
     3301 ccacatttcc tcacggggca ccttgccttc tcgctgcagc cagtcgaaaa cggcaggcat
     3361 tttccaggtg gatggatcga tcgcgaggcc gagtccttcg ggcaccacgc gtatgatgtt
     3421 ttcggtgagg ccgccgcccg tgacgtgggc catgccgtgt accagaggga ccggagaccg
     3481 gggaccgggg accggggatc cggaggcgtg gagcaattgg aggatgggct tgacgtagat
     3541 ggtggtgggc gccatgaggg catccgccaa tgtgacgccg tcgagttcca ggtcgaatgg
     3601 cgagcccgcg cgctcgacga tcttgcggat cagcgagtag ccgttcgagt gcgggccgct
     3661 ggaggcgacg ccgaggatca cgtccccggc gcggatggcg ctgccgtcga ccagcgcgga
     3721 tttttccacc cccgccacgc agaatccggc gagatcgtat tcaccgggcg gatacatgtc
     3781 cggcatctcg gcggtctcgc caccgaccag cgcacagccg gcgagttcgc aaccgcgcgc
     3841 gatgccgccg accaccgcga cggtggtatc cacgtcgagc ttgccggtcg cgaaatagtc
     3901 gaggaagaac aagggctcgg caccctgcac cagcacgtcg ttgacgcaca tgcccacgag
     3961 gtcgataccg atggtgtcgt ggcgattcaa ctgttgcgcg agtttcagtt tggtgccgac
     4021 gccgtcggtg cccgaaacca gcaccggttc gcggtatttc ttcgagaggt cgaacaacgc
     4081 gccgaagcca ccgaggccgc ccaacacctc ggcgcgggat gtcctcgcga ccagtggctt
     4141 gatgcgttcg accacggcat tgccggcgtc gatgtccacg cctgcggcgc ggtaggagag
     4201 ggcgtcgttg ctggaagtca cgatggcatc ggcggcaagc gccgcgaagt gcgggaccgg
     4261 caatgataga cgctggcgcc gcgattgggc agactggcgt ctggctgatc ggagagtacc
     4321 tggccatgac gcgcttcctg ttttccctgc tgctggtctt cggcgcggcc gccgctgcgc
     4381 cggcccagac cacgccggat accgccgtgc catccgccgt tcccgcgaat ccctactcga
     4441 ccacggtccc ggtcgcgggc accagcgacg cccagcgcga tgccgcgatc tcggcggcgc
     4501 tggccaatgt cctgcaacgg gtttcgccgg gtttcgccgc cccgcccgaa gtgctcgccc
     4561 aggcatcggg ctacgtgcgc gcctaccact accgccgtgc ggcttccgga gggctggaac
     4621 tgcaggtgga tttcgatccc ggcgcggtcg gaaggatggt cgcccaggca gccaacgcgg
     4681 cgggcggcgc gctcgccggg cagccggggg tggccggttc ggcgggcgcc gttgccgcgg
     4741 ctccggccgg cagcgcgacc gtctgggtgg agggcatcga aaacgaccat gcgctcgcga
     4801 cgctgctctc cacgctgcgc aatgatccct cgatgagcga cgtggttccg gtcggagcca
     4861 agggcgacgg cgttttgctg cagctgacct ccgaccagcc gctggcgact gcgctggcgg
     4921 cggtcgcggg tcccacgggg cacctcgcac cggtcgcgca gccgcatccg ggcgccgatg
     4981 ccaccttccg ctgggtgcca tgaacatgcc tgggtcgtga gcggaacgcg cgcgaccgac
     5041 gacgggtcgc cggacgggcc gctgcggcgc ctgccgaatg ccgtatcgtg cttgcgcatc
     5101 gccctggttg caccgctgat cgtgacgatc ctcgccggcc gccatgaaat cgcgctggtg
     5161 atcgccggcg tcgccggggt ctcggatgcc gtcgacggct atctggcgcg gcgcttccgc
     5221 tggcagagcc ggttggggtc gatcctcgac ccggccgcgg acaagttgat gctggtgggc
     5281 tgcctggtcg cgttgggctg gatgcaggag gcgccgcgct ggctggtcgc gctggtggtg
     5341 gcgcgcgacg cggtgatcgc gctcggcgcg ctggcgtggc atcgtatcct gcacacgttc
     5401 gaggcccgcc cgagctggct gtcgaaggcg acgaccgtgg cgcagatagg atacgtcctg
     5461 ctggtgctag ccgaccgcgg ttttgcgtgg caggtgccga tggcgatccc ggtgtggatc
     5521 gtcgcggggc tgacggcggc gagcgggctc gattacgtgg tgcgctgggg gctgctggcg
     5581 cggcgggaac tcaacgcggg gaaacattcg acgtgaccaa cgcacagcgc tggcaatggc
     5641 tgatcatcgc tgccgtcgtg ggttggctgg tctggctgtt gtcgccgatc ctgatgccgt
     5701 tcgtgctggc gggcgtgttc gcctatctcg gcgatccgct ggtggatcgg ctggaacgac
     5761 tgcgcctcgg ccgcgggctt gcggcgagcc tcgtgttcct gttgatgctc ttgatcgtcg
     5821 cgttcgcgct ggtcctgctg gtgccgttca tccagcgcca ggtcgcgaac ttcctggcgg
     5881 cactgccggg ctacggcgcg tggatcaaca accacgcgat tccttggctg gaggagaaac
     5941 tgcacatccc ggccagcgaa ttcgacgtca acacgatgat cgcgcggctg cgcggcaacg
     6001 ccgggacggt gggcggcgtg gtcacggcgg tgctcgccta cgcgacccgt tccggcatcg
     6061 cgctgatcgg cgtcggcgtc gcggtggtgc tggtgccggt ggtgacgttc tatctgctgc
     6121 gcgactggga caagctggtc gcgcacgtcg acgagttgct gccgcgcgag gcacagccga
     6181 cgatccgccg gctcgcgcgg gaaaccgaca gcgtgctcgg tgccttcgtg cgcggccagt
     6241 tgctggtgat ggtggggctg gccgtttact acgccgtggc gctgcgcctg gtcggactcg
     6301 aggtcggccc gctgatcggc atgatcgcgg ggctggtcag cttcgtgccg tacctcgggt
     6361 tcatcatcgg catcctcgcg agcatcgtcg cggcgctggt gcagttccac gacggctacc
     6421 acgtcgtgct ggtgctggtg gtgttcggga tcggcaacct gctcgaaagc tacgtgctgg
     6481 tgcccaagct ggtgggcgac cgcatcggat tgcatccggt cgcggtgatc ttcgcggtac
     6541 tggcgttcgg ggaattgttc ggcttcatcg gcatcctgtt ggcactgccg atggcgtcga
     6601 tcgcggtggt gttgctgcga ttcctgcgcg aacgctatga ggcgagcgcg ctgtatgccg
     6661 gtcgcggccc cccgaagatc gtcgggtcgc aagggccgcc aggcccgggc ccgggtgcgg
     6721 gcaccgacgg tctcgcgaat ccacgcggcc catccggcga gacatgatcc ggcagtttcc
     6781 gctcgacctg cgctggccac cacaccagcg cctcgacgta ttccatgccg cgggcaatgc
     6841 cggcgccgtg cgcgaggtcg aggccttggc gatccagccg tcgcacgcct gggtgttcct
     6901 gcacggcgcg tcggggaccg gcaagagcca cctcctgatc ggcgcctgcc gcgcggcggc
     6961 ggaggccggt cgcgaggcgc gttacgtgtc actggcatcg ctgccgggat ctcgcgccga
     7021 agcgatcgcc gggatcgatg gcgaaggcct ggttgccatc gacgacgtgc actcgctggc
     7081 cggcgaccgc gatgccgagc gcgcgctgtt cgacctgttc aaccgtgcgg cggcagcgtc
     7141 ggcccgcatg ctgttcgcgg cgaatgcatc cccggcggaa ctcggcatcg ggctcgcgga
     7201 cctcgtgtcg cggctgtcct cgtgcacgca gtccgcattg catccactcg atgatggcgg
     7261 gcggcgcgcg atgttgcgct tgctcgcagg gcgcatggga ctgcggctag acgacgaagt
     7321 gctggactgg tggttcgcgc gccagccgcg cgatcccgcg tcactggtcg cattgctgcg
     7381 gcggatcgat cgcgcggcac tggccgcgca gcggcgcgtc acgattccgt tcctgcggga
     7441 gctcttgcgc gagtagccgg gattctttgt aggagcggcc catggccgcg acgacgcgag
     7501 cgcgaggtgg atcggtcgcg cgcaacggca aaaggcggcg cttcgcgccg gcgcgcctct
     7561 acgagggcag gtccgcgttg agctcggcca ggcgcgcggg cgtgccgatg tcggtccagg
     7621 cgccggcatg gtgccgccat ccgacttcgc cacgtgcgat cgcggcgcgc agcagcggga
     7681 cgatcgaaaa gcgcggcggg gtcacctgcg cgccgggcgt atcgccgatg atgccccgcc
     7741 agtcgccgag gacttccgcg cggtagatgc cgatcccggc gaaggtcagc gcacgcgcgg
     7801 cgccgggcga atccgcgtgg ggcaacagcg ttcccgttgc atcgattccg aaatcgccgc
     7861 gcgggtgctg cggcggattg tccaccagca ccagttccgc gagcccctgc ggctcgcgcg
     7921 gcaaggatgc aaaatcgaag tcggtgaaga tgtctccgtt gaccgcgatg aacggggcct
     7981 tgcccagcaa cggcagcgcg tgcagcatgc cgccaccggt ttcgagcggc acgtcgcctt
     8041 cgcggctgta ttcgatgcgc aggccgtagc gcgcaccgtc gcccagcgct tcggggaatt
     8101 gggcggcgag gtgggcgatg ttgatgacaa cgtcgtgcac gccggccgcg gcgaggcgtt
     8161 cgaggtgcca ttcgatcagc cgcttgccgg cagccgcgag caggggtttg ggcgtggtgt
     8221 cgctgagcgg gcgcatccgt tcgccgcggc cggccgcgaa gaccagcgcc ttcatggcga
     8281 acgcccgtgc ctggtggggg aaagaccccc ttcagcccgg cacccgtccc gtgggcgaga
     8341 gggagcaaag cgcgcaggga ccgggctgcg aaatcgctcg atcacttcga gggcgccgtc
     8401 aggtcgcgtt cgcccaccca gcgctccagc agcctgagga agtcttcgag ttcggggtaa
     8461 tcccaggcga cgctg
//
LOCUS       DASSNH010000002         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_714742, whole genome shotgun sequence.
ACCESSION   DASSNH010000002 DASSNH010000000
VERSION     DASSNH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714742"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..402)
                     /locus_tag="VFK00_00055"
     CDS             complement(46..402)
                     /locus_tag="VFK00_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7591381.1"
                     /translation="MPIYVFRCEDCGEQFDRLQKLSDPDPDTCPNCGRHHVRRQVTAP
                     AFRLAGSGWYETDFKSDKEKKRNLAGRDESASGNGAKAGDKPAAGEKPTSGGGDAKTA
                     NAAEPKAAGTSAASKD"
     gene            523..1182
                     /locus_tag="VFK00_00060"
     CDS             523..1182
                     /locus_tag="VFK00_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7591382.1"
                     /translation="MLFVRNTVLAALASFLTPAAFAAEVLCPATPPPQPAAQVVHVRD
                     VNRGLGIPADYTARHHLALQPEAKDLVQVPRDVYGRKVEMVAPAATALKKMFAAAKHD
                     GVELQTVSAFRSMSYQTGLIRKKLKRGMSIEKALAINAVPGYSEHQTGCAVDLTTPGV
                     PAADASFAKSKAFGWLERNAGRFGFHLSFPPGNQYGYEYEPWHWRYIANTASPSSPGQ
                     R"
     gene            complement(1410..2708)
                     /locus_tag="VFK00_00065"
     CDS             complement(1410..2708)
                     /locus_tag="VFK00_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET7591383.1"
                     /translation="MNVSTRRCPALLVSAPASGQGKTSVTAALARWHVRQGRCVRVFK
                     TGADFLDPMITGRAAASPAYQLDWWMGGEADVRARLHAAAAEADLILVEGVMGLFDGD
                     PSSADLAAACGLPVLAVIDGGAMAQTFGAVAQGLAEFREDVRVYGAAANRIGGEYHAQ
                     LLHKSLPNGIAWLGALPDDPALVLPERHLGLVSAGELADLDARIDALADAWSRYAEPG
                     LPPPARFKPAPSASAPPLLQGSRIAVARDDAFCFIYPANLDLLRDAGADLRFFSPLAG
                     DALPECDAAWLPGGFPELHLQVLSQRSDLHAALRGHVMAGKPLLAECGGLLFALDDLA
                     CADGAAASMAGLLQGRAVVHPRLAALGLQEVALPEGTLRGHTFHHARAEIEAKPIATA
                     VNPNGAPSREAVYRERRFTASFVHFYFPSDPEAALRLFLP"
     gene            complement(2705..>3871)
                     /locus_tag="VFK00_00070"
     CDS             complement(2705..>3871)
                     /locus_tag="VFK00_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7591384.1"
                     /translation="DDALSVHGAYQFNEQWSADGLAFRSQGRNEYDGTFSDADDYSNQ
                     VLGGQLHFLPSKRVKLSLRAGSSTDFDADFLGGVYVDNFDTRRTLGSLQADIASGGGL
                     LTLGYDWVREHLASNTLYVENARIDRGLFAQWQRDFAAQSLQANLRRDQDSQFGGKTT
                     GGLLWGWDFAAGLRLTASYGTAFRAPTFNDLYYPGFGNPSLRPESSRNLELGLRGTPA
                     WGHWQVSAYRNDIHDMIAFDAAVGLPGNVDQARITGIEGVVDGKVAGWNLRATATLLH
                     ARNDARGGFNDGNELPRRPSRSARFDADRSFGRLSLGASWYLAGRRYDDIANLHPLGG
                     YALVNVRAGWQFGRAWALQLALDNVFDKDYETAWFYDQPGRNFMLTLRYRPNPP"
BASE COUNT          634 a         1339 c         1315 g          583 t
ORIGIN      
        1 ccacatgcgg gcggcaggta ggtccgccct gggacgaggc cgccgtcagt ccttcgaagc
       61 cgccgatgtc cccgcagcct tgggctcggc cgcgttggcg gtcttcgcgt cgccgccgcc
      121 ggaggtcggc ttctcgccgg cggcgggctt gtcgccggcc ttggcgccgt tgcccgacgc
      181 cgactcgtcg cggccggcga ggttgcgctt cttttccttg tcggacttga agtcggtctc
      241 gtaccagccg ctgcccgcga ggcggaacgc cggcgcggtc acctggcggc gcacgtggtg
      301 cctgccgcag ttcgggcagg tgtcgggatc gggatcggag agtttctgca ggcgatcgaa
      361 ctgctcgccg caatcctcgc aacggaaaac gtagatgggc atgacagggg aaaacgcaaa
      421 tcgcaacctt gcaaggatac tccaatcggc gatccgcgtg agcggtccgg aacgccggac
      481 cggctcccct atcatgcgcg tttcgccatt ccggagatcc gcgtgctttt cgtccgcaac
      541 accgtgcttg ccgccctcgc gtcgttcctc acccccgccg ccttcgcggc cgaggtgctc
      601 tgcccggcca cccctccgcc ccagcccgcg gcgcaggtcg ttcacgtccg ggacgtgaac
      661 cgtggtctcg ggattccggc ggactacacc gcacgccacc acctggcgct gcagccggaa
      721 gccaaggacc tggtgcaggt gccacgcgac gtgtatggac gcaaggtcga gatggtcgcg
      781 cccgcggcca cggccttgaa gaagatgttc gcggccgcga agcacgacgg ggtcgaactc
      841 caaacggtgt cggcctttcg cagcatgtcg taccagaccg ggctgatccg caagaagctg
      901 aagcgcggca tgagcatcga gaaggcactg gcgatcaatg cggtgccggg ctacagcgaa
      961 caccagactg gctgcgccgt cgatctgacc acgcccggcg ttccggccgc cgacgcgagc
     1021 ttcgccaaga gcaaggcgtt cggttggctc gaacgcaacg cgggacgctt cgggttccat
     1081 ctctcgttcc cgcccggcaa ccagtacggc tacgagtacg aaccgtggca ctggcgctac
     1141 atcgccaaca ccgcgagccc gtcgtctcca gggcaacgct gacgcaggag cggtcgatgg
     1201 ccgcgaccag ccgcgcggcg gatcttcgcc aagcttcgcg ccaacacgac tttgtaggtt
     1261 ggtgccgagc ggggttttat cccgcggagc gcaacgcggg ggcgaccgat ccccgttggg
     1321 cttctcccgg acaaatccgg gatcagccca gcctacgaaa gcctcatccc gccaacacca
     1381 acgcgcgatc gatgcctgtt ggaatcaggt cacggcagga acaatcgcaa tgcggcttcc
     1441 gggtccgacg ggaaatagaa gtgcacgaaa ctcgcggtga agcgtcgctc gcgatacacc
     1501 gcttcgcgcg acggcgcgcc gttggggttc accgccgtcg cgatgggttt cgcctcgatt
     1561 tccgcgcgcg cgtggtggaa ggtgtggccg cgcagcgtgc cttccggcag cgccacttcc
     1621 tgcagcccca gcgcggcgag ccgcggatgc acaacggcac gcccctgcaa caggccggcc
     1681 atcgatgcgg cagcgccgtc ggcacaagcc aggtcgtcca gcgcgaacag caaccctccg
     1741 cattcggcaa gcagcggctt gccggccatc acgtgcccac gcagcgcggc gtgcaggtcg
     1801 ctgcgctgcg acaacacctg cagatgcaac tcgggaaaac cgccgggcag ccaggcggca
     1861 tcgcactcgg gtagcgcatc gcccgccagc ggcgagaaga accggaggtc cgcacccgcg
     1921 tcacgcagca ggtcgaggtt ggccggatag atgaaacaga acgcgtcgtc gcgtgcaacg
     1981 gcgatgcgcg agccctgcag caacggcggc gcggatgcgg acggcgcggg cttgaacctc
     2041 gccggcggcg gcaggccggg ttccgcgtac cggctccagg catcggccaa cgcatcgatg
     2101 cgtgcatcga gatcggccag ctcaccggcg gacaccaggc cgagatgccg ctcgggcagc
     2161 accagcgcgg gatcgtccgg cagcgcgccc agccatgcga tgccgttcgg gagactcttg
     2221 tgcaacaact gcgcgtggta ttcgccgccg atgcgattgg cagcggcgcc atacacccgc
     2281 acgtcttcgc ggaactccgc cagtccctgc gcgaccgcac cgaacgtttg cgccatcgcg
     2341 ccgccgtcga tcaccgccag caccggcaac ccgcaggcag cggcgaggtc ggcgctggag
     2401 ggatcgccgt cgaacaggcc catcacgcct tccaccagga tcaaatcggc ttcggccgcg
     2461 gccgcgtgga gccgcgcacg cacgtcggct tcgccgccca tccaccagtc gagctggtag
     2521 gcaggcgatg cggcggcacg ccccgtgatc atcggatcga ggaaatccgc gccggtcttg
     2581 aacacccgca cgcaccgccc ctgccgcacg tgccaacgcg ccagcgccgc ggtcacgctg
     2641 gtcttgcctt ggcccgaggc gggcgcggaa accagcaggg ccggacaccg gcgcgtggaa
     2701 acgttcatgg cgggttcgga cgataccgca acgtgagcat gaagttgcgc cccggctggt
     2761 cgtagaacca ggccgtctcg taatccttgt cgaacacgtt gtcgagggcg agttgcagcg
     2821 cccaggcgcg gccgaactgc cagccggccc gcacgttcac gagcgcatag ccgcccagcg
     2881 ggtgcaggtt cgcgatgtcg tcgtagcggc ggccggcgag ataccagctc gcgccgaggc
     2941 tgaggcgccc gaagctgcgg tcggcgtcga aacgcgcgct gcggctgggg cggcgcggca
     3001 actcgttgcc gtcattgaac ccgccccggg catcgttgcg cgcatgcagc agcgttgcgg
     3061 tcgcgcgcag gttccagcca gcgaccttgc cgtcgaccac gccctcgatg cccgtgatgc
     3121 gggcctggtc gacattgccc ggaaggccca ccgccgcatc gaacgcgatc atgtcgtgga
     3181 tgtcgttgcg gtaggcgctg acctgccagt gtccccacgc cggggtgccg cgcaggccga
     3241 gttccaggtt gcgcgaactc tcggggcgca gggacggatt gccgaagccc gggtagtaga
     3301 ggtcgttgaa ggtcggtgcg cggaatgccg tcccgtagct cgcggtgagc cgaagcccgg
     3361 cggcgaagtc ccatccccac aggaggccgc cggtggtctt gccgccgaac tggctgtcct
     3421 ggtcgcggcg caggttggcc tgcagcgatt gcgcggcgaa atcgcgttgc cattgcgcga
     3481 acaggccgcg gtcgatccgc gcgttctcga cgtacagggt gttgctcgcg aggtgctcgc
     3541 gcacccagtc gtagccgagg gtcagcaggc cgccgcccga cgcgatgtcg gcctggagcg
     3601 agcccagcgt gcgacgggta tcgaagttgt cgacgtagac cccgccgagg aaatcggcgt
     3661 cgaagtcggt gctggaaccg gcgcgcagcg acagcttcac gcgcttgctc ggcaggaagt
     3721 gcaactggcc gcccagcacc tggttcgagt aatcgtccgc atcactgaag gtgccgtcgt
     3781 attcattgcg gccctggctg cgaaacgcca ggccgtcggc gctccattgc tcgttgaact
     3841 ggtaggcacc gtgcacgctc aaggcgtcgt c
//
LOCUS       DASSNH010000003         8906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_374753, whole genome shotgun sequence.
ACCESSION   DASSNH010000003 DASSNH010000000
VERSION     DASSNH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374753"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /gene="mlaE"
                     /locus_tag="VFK00_00075"
     CDS             <1..681
                     /gene="mlaE"
                     /locus_tag="VFK00_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HET7591385.1"
                     /translation="ALPKSLRYWRETCRQVWFVGAMSLVIVMTCGLFVGMVLALQLYY
                     TLSIFGGTAALGTVVALSLFRELGPVVTALLVAGRAGTAVTAEIGLMRATDQLDAMSM
                     MAVDPLAYVASPRFLAGVIAMPLLACVFNAMGIFGGHLVGVTWLGLDNGTFWGNMTST
                     VDLHKDILDGLYKSIVFGGVVSLIAVFQGYTAPPTSEGVAYSTTRTVVFSSIVILAFD
                     FVMTAFLT"
     gene            703..1224
                     /gene="mlaD"
                     /locus_tag="VFK00_00080"
     CDS             703..1224
                     /gene="mlaD"
                     /locus_tag="VFK00_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET7591386.1"
                     /translation="MNPRPSYAIGTGLFILLGFAALAYLATQTTSVANGMTSSNTYAV
                     KARFGNIGQLKERAPVKIAGVRIGDVQSITLEPQRMDALVVMSIDKRYSDIPEDSAAA
                     IFTSGLLGDQYVGIQPGGSPEFLKDGDEMVLTQSAMSIENLIGKFMVNGGSSSSGSGA
                     APASSAPPASRKP"
     gene            1269..1919
                     /locus_tag="VFK00_00085"
     CDS             1269..1919
                     /locus_tag="VFK00_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7591387.1"
                     /translation="MPKRFIPLFLAFALAALAAPAVAAPTGQAPQQVVQSIADKLADA
                     IQGRQAELRQNPDEVIKIIDGIVLPHFDMDYAALLVLGPHAREATPAQRVAFTRAFYK
                     ALTHRYAEGLVAYTRGAVKVLPAQGPLDQRRTIVRTQVQLASGKNLSVDYAFRKTSSG
                     EWKAYDVIIEGISYITNYRNQVDAEIQKEGIDGLIKRLQTQGAAAINEMKKEGGGH"
     gene            1922..2257
                     /locus_tag="VFK00_00090"
     CDS             1922..2257
                     /locus_tag="VFK00_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7591388.1"
                     /translation="MVLTSAPADTVEIGAADRDTLAIRGALTFATAQRACTDGERVLA
                     AGSQTRLDLAGIAHADSAGLACVIALAAAANRAGRRLRVVALPEGLRALAEVCGVAAL
                     LEPDARPAA"
     gene            complement(2267..3199)
                     /locus_tag="VFK00_00095"
     CDS             complement(2267..3199)
                     /locus_tag="VFK00_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HET7591389.1"
                     /translation="MPRTRRSRAAQHIIIACLAGALAACAVAPPRTDDPLQSFNRKMF
                     AFNRFADKAAIRPAAKAYVKVTGPKERILIGNFFSNLRTPITIVNEVLQGRPGPALQS
                     TGRFLINTTVGFLGVFDPASEMKLPPHPTDFAVTLAHWGVPEGPFLVLPFIGPTTARD
                     VWRLPVDSYFDPLGWYARNHDFRWNAQYLPNLAYLVTLRANALPLDPIIDTSYDPYAF
                     TRDAYRQNRLYQVYYGNPPLSAIEQLQGTAPGQENGEDLDKLLEQQKAYEHSHGLNQN
                     GMPASGGSSAQPAPAASNGLPAPPTSSAVPAPAG"
     gene            3402..3695
                     /gene="rpoZ"
                     /locus_tag="VFK00_00100"
     CDS             3402..3695
                     /gene="rpoZ"
                     /locus_tag="VFK00_00100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7591390.1"
                     /translation="MARITVEDCLKVVDNRFELVLMATRRARQLENGVEATIDPENDK
                     PTVLALREIADHTIDMPTIDEIDRVERERRDREALEWAAAEVDEDMAKGVDEL"
     gene            3782..5968
                     /locus_tag="VFK00_00105"
     CDS             3782..5968
                     /locus_tag="VFK00_00105"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7591391.1"
                     /translation="MRKRKAKAGANDLRVPGRDTEPAEVRAIDALAEQLAAYLPPEQI
                     ERVRGAYAVSAHAHASQRRKSGEPYITHPLAVASILAGLRLDAETIIAAILHDTLEDT
                     GLTRVQLEGGFGATVTELVDGVTKLERVDFASRVEADAESFRKMLLAMARDLRVILIK
                     LADRLHNMRTLSAMAPDARRRIARETLDIYAPIAARLGMNAIRSELQDLGFRSLYPDR
                     YKIIASRIKRTIGDRRESMAIIEKALAERLAADGVPARIISRVKAPYSIWSKMKSEHK
                     TFAEVMDVYGVRVVTDTVAHCYQALGSAHSLFKPVEGRFKDFIAIPKANGYQSLHTVL
                     FGPFGAPIEVQIRSEDMNTVAERGVAAHWSYKTETTPPNSAQLRARAWVETLADNQAA
                     TPSSLEFLENVKVDLFPDEVYLFTPAGDIQALPRNATALDLAFAVHTDVGMHAVASRI
                     DGKLASLRSQPSSGQTVEVITAPSAAPNPQWLDFAVTGKARAAIRQYLKRLQHEDAID
                     FGHRMLESALDTRGSSLDAVLPEVLDRFLAEHSLRRLEDLLSDIALGNRMPDQVAAKL
                     TEMGGPVIGAALHRSEKIRISGAERGILSFGNCCHPLPGDDIVGYLSPGKGIVVHRVE
                     CPNVVELAKLPERRVEIGWDKEVQGDYRAELRVEVVNRPGVLATVSAAIAEANSNIDD
                     VKYIERGIQAATLIFAIEVKNRKHLADVMRRIRNTKVVNGVYRYPL"
     gene            6001..7644
                     /locus_tag="VFK00_00110"
     CDS             6001..7644
                     /locus_tag="VFK00_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591392.1"
                     /translation="MAYLADVLFPARKAMAELRARWAKPGDKDGWRDSDYFDLVRDRE
                     ASSSIDDKTWVDLEYPKIFASMDSTVTPVGSQVLYRRMREYSGDAATLAANYAVCAEL
                     RSNAALRETLQRRLLPLKDESNNRIVDFVFGKLPPAPRHQALLWSWSLASAAVLGGVV
                     WWAWPAWIWVAMLFVNAFLIFRACADSLRDAETLTQCLLLVKVADDLAALHEAHRSLP
                     QLAGLHGEASNRRAVRKALRWLARLKRPESAFVFVWLNFAFLLELLAHGRAMRRFAVL
                     RHKLAASFELVGEIDAMIAMASWLAYRPDHCHPSFSDCAMLDIEDGGHPLLEAGVRNS
                     IRLEQRSALVTGSNMAGKTTFIKMVGVNVLLGQTLGFCLAARATIPHAPVMASIQAGH
                     SVASGKSHYFSEVETIRSFFARQADQRRAIFVIDELFTGTNTTERVAVARAVLESLGE
                     GSIVLATTHDVELQALLDERYDLYHFQESPDVDGFFDYRLRAGPASERNAIRLLGELG
                     FPARIVAKAMRYAREELAGEKRGADVPRRGVRTKPVGSA"
     gene            7722..8108
                     /locus_tag="VFK00_00115"
     CDS             7722..8108
                     /locus_tag="VFK00_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7591393.1"
                     /translation="MPRQPIHSDSAPKAIGPYSQAVRSGNTLYLSGQTPLDPATGEMV
                     AGGIDEQAERVFENLKAVLGAANATFDDVVRVAIYMTDLGNFSAVNEVMKRYFNEPYP
                     ARSTIGVAALPRGAAVEIDVIAAVDR"
     gene            complement(8149..8715)
                     /locus_tag="VFK00_00120"
     CDS             complement(8149..8715)
                     /locus_tag="VFK00_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591394.1"
                     /translation="MSEFEYITVLLSIILGLAVTQLLAGFARLLRDGRSLTPAWWIFV
                     LIAMLLLANLQIWWVSHEWRRVEEWTFLAYAAFMILPVLMYLLAYLLLPDNLHLTGHE
                     LAREFVDHRKPFYTCLALVPLASFLQERLLTGHLPSASLDTGFRLVFLAIAVPGFLWR
                     QTRVQACVALLALLNMATYTGVLFTQMR"
BASE COUNT         1520 a         2941 c         2992 g         1453 t
ORIGIN      
        1 gcactgccca agagcctgcg ctactggcgc gagacgtgcc ggcaggtctg gttcgtcggc
       61 gcgatgagcc tcgtgatcgt gatgacctgc gggcttttcg tcggcatggt gctggcgctg
      121 cagctttact acacgttgtc gatcttcggc ggtaccgccg cgctcggaac cgtggtcgcg
      181 ctgtcgttgt tccgcgaact cggtccggtg gtgaccgcgc tgctggtggc ggggcgtgcg
      241 ggcaccgcgg tcaccgccga gatcggcctg atgcgcgcga ccgaccagct cgatgcgatg
      301 tcgatgatgg ccgtcgatcc gctggcctat gtcgcctcgc cgcggttcct cgccggcgtg
      361 atcgcgatgc cgctgctcgc gtgcgtgttc aacgcgatgg gcatcttcgg cggacacttg
      421 gtcggcgtga cctggctcgg gctcgacaac ggcaccttct ggggcaacat gacctccacg
      481 gtcgatttgc acaaggacat cctcgatggc ctgtacaaga gcatcgtgtt cggcggcgtg
      541 gtcagcctga tcgccgtgtt ccagggctac accgcgccgc ccaccagcga gggcgtcgcg
      601 tattcgacca cccgtacggt ggtgttttcc tcgatcgtga tcctcgcctt cgatttcgtg
      661 atgacggcgt tcctgacctg accccgtagc attggtgact gcatgaatcc gagaccttcc
      721 tacgccatcg gcaccggcct gttcatcctg ctcggcttcg ccgcgctggc ttaccttgcc
      781 acccagacca cctcggtggc caacggcatg accagcagca acacctacgc cgtcaaggcg
      841 cgcttcggca acatcggcca gttgaaggaa cgcgcgccgg tgaagatcgc gggcgtgcgg
      901 atcggcgacg tgcagtcgat cacgctggaa ccgcaacgga tggatgcgct ggtggtgatg
      961 tcgatcgaca agcggtacag cgacattccg gaggattccg cggccgcgat attcaccagc
     1021 ggtttactgg gcgaccagta cgttggcatc cagccgggcg gctcgccgga gttcctgaaa
     1081 gacggcgacg agatggtgct gacccagtcg gcgatgagca tcgagaacct gatcggcaag
     1141 ttcatggtca acgggggatc gagctccagc ggttccggcg cggctcccgc gtccagcgcc
     1201 ccgcccgcat cccgcaagcc ctgacatttc gcgctgcgac ccgtcgcacg ctcccgagga
     1261 gacattccat gcccaagcgt ttcatcccgt tgttcctggc gttcgcgctc gcagcgctcg
     1321 ccgcgcccgc ggtggcggca ccgaccggcc aggctccgca acaggtcgtc cagtcgatcg
     1381 ccgacaagct ggccgatgcg atccaggggc gccaggccga gttgcgccag aatcccgacg
     1441 aggtgatcaa gatcatcgat ggcatcgtgc tgccgcattt cgacatggac tatgcggcgc
     1501 tgctggtgct cggcccgcat gcgcgcgaag cgaccccggc gcagcgcgtg gcgttcacgc
     1561 gggcgttcta caaggcgctg acccatcgct atgcggaagg gctggtcgcc tacacccgcg
     1621 gcgcggtgaa ggtgttgccg gcgcagggcc cgctggacca gcgccgcacg atcgtgcgaa
     1681 cccaggtgca gctcgcgagc ggcaagaacc tgtcggtgga ttacgcgttc cgcaagacct
     1741 cttccggtga atggaaggcg tacgacgtga tcatcgaggg catctcctac atcaccaact
     1801 accgcaacca ggtggatgcc gaaatccaga aggaaggcat cgacggtttg atcaaacggc
     1861 tgcagaccca gggcgcggcc gcgatcaacg aaatgaagaa ggaaggcggc gggcactgac
     1921 gatggtcctc acgtccgccc ccgccgacac ggtggagatc ggtgccgccg atcgcgacac
     1981 gctcgcgatt cgcggcgccc tgaccttcgc cacggcgcag cgcgcctgta ccgatggcga
     2041 gcgcgtgctg gccgcgggct cgcaaacacg cctggatctc gcgggcatcg cgcacgccga
     2101 cagcgcgggg ctcgcctgcg tgatcgcgct ggctgccgcc gcgaaccggg cgggccgacg
     2161 cctgcgcgtg gtcgcgctgc cggaagggct ccgcgcgctt gcggaggttt gcggcgtggc
     2221 cgcgctgctg gagcccgacg cgcgtccggc tgcttgatcc gccggctcag cccgcgggtg
     2281 cgggcacggc gctggaggtt ggcggcgccg gcaacccgtt gctcgctgcc ggcgccggtt
     2341 gcgccgacga tccgccggat gccggcatcc cgttctggtt caggccgtgc gagtgctcgt
     2401 acgccttctg ttgctcgagc aacttgtcca ggtcctcgcc gttctcctgc cccggggcgg
     2461 taccctgcag ttgttcgatc gccgacagcg gcgggttgcc gtaatacacc tggtacaggc
     2521 ggttctggcg ataggcgtcg cgcgtgaacg catacgggtc atacgaggtg tcgatgatcg
     2581 gatccagcgg cagcgcgttc gcgcgcaggg tcacgaggta cgcgaggttc gggaggtact
     2641 gcgcgttcca gcggaagtcg tggttgcgcg cgtaccagcc gagtggatcg aaatagctgt
     2701 ccaccggcag ccgccacacg tctcgcgcgg tggtggggcc gatgaacggc agcaccagga
     2761 aaggcccctc cggcacgccc caatgcgcca gcgtgaccgc gaagtcggtc gggtgcgggg
     2821 gcagcttcat ctcgctggcc gggtcgaaca cgcccaggaa tccgacggtg gtgttgatca
     2881 ggaagcgccc ggtcgattgc agcgcaggcc cgggacgtcc ctgcagcact tcgttgacga
     2941 tcgtgatggg cgtgcgcagg ttcgagaaga agttgccgat caggatccgt tccttcggtc
     3001 ccgtcacctt cacgtaggcc ttcgcggcgg gccggatcgc cgctttgtcc gcgaaacggt
     3061 tgaaggcgaa catcttgcgg ttgaacgact gcagcggatc gtccgtacgc ggcggcgcca
     3121 ccgcgcacgc ggccagcgcg cccgcgagac aggcgatgat gatgtgttgc gcggcgcggg
     3181 agcggcgggt gcgtggcatg cgtcgtggat gcgggcggcg ggcggaagtt gcaagtgcgc
     3241 gattgtacgg gccttcgcgc cgcgaacgcc ggggcgaagg gttcctggag caggcggcgt
     3301 tcaggcgggt ccgccacggc tggcggccgc aatcccgccg catcgacaaa tgccggcgtg
     3361 ctgctacaat ctggaagttt gcagcatgac cggaacccca tatggcccgc atcaccgtcg
     3421 aagactgcct gaaggtggtc gacaaccgtt tcgaactggt cctcatggcg acgcgccgcg
     3481 cgcgccagct cgagaacggg gtcgaagcca ccatcgaccc cgaaaacgac aagcccaccg
     3541 tcctggccct ccgcgaaatc gcggaccaca ccatcgacat gccgaccatc gacgagatcg
     3601 atcgggtcga gcgtgagcgc cgcgaccgcg aggccctcga atgggcagcc gcggaagtcg
     3661 acgaagacat ggctaagggc gtcgacgagc tctgacgcac ggatcgcctt cgcaagggcg
     3721 tccgtcttcc gccggcgtgg tcgggattga ctcgcacgca cggcatgtct agcttgcggt
     3781 gatgcgcaag cgcaaagcca aagccggggc caacgacctg cgggtgccgg gacgggacac
     3841 cgagcccgcc gaggtcaggg ccatcgacgc gctcgcggaa cagctcgcgg cctacctgcc
     3901 ccccgaacag atcgagcggg tgcgcggggc gtacgccgtg agcgcgcacg cccacgccag
     3961 ccagcgcagg aagagcggcg agccgtacat cacccacccc ctcgcggtgg cctccatcct
     4021 ggccggcctg cgcctggatg cggaaaccat catcgcggcg atcctgcacg acaccctcga
     4081 ggacaccggc ctgacccggg tgcagctcga gggagggttc ggcgccacgg taaccgagct
     4141 ggtcgacggc gtcaccaagc tggaacgggt ggatttcgcg agccgcgtcg aagccgatgc
     4201 ggaaagcttt cgcaagatgc tgctggcgat ggcgcgcgac ctgcgggtga tcctgatcaa
     4261 gctggccgac cggctgcaca acatgcgcac gttgtcggcg atggcgcccg acgcgcgccg
     4321 ccggatcgca cgtgaaaccc tggacatcta cgccccgatc gcggcgcgcc tcggcatgaa
     4381 cgcgatccgc agcgagctgc aggacctcgg gttccgcagc ctctaccccg atcgctacaa
     4441 gatcatcgcc tcgcggatca agcgcaccat cggcgaccgc cgcgaatcga tggcgatcat
     4501 cgagaaggcc ctcgccgagc ggctggccgc ggacggcgtc cccgcgcgga tcatcagtcg
     4561 cgtcaaggcg ccctacagca tctggtcgaa gatgaagagc gagcacaaga ccttcgccga
     4621 ggtgatggac gtgtacggcg tgcgggtcgt gaccgacacg gtggctcact gctaccaggc
     4681 gctcggttcg gcgcattcgt tgttcaagcc ggtggagggg cgcttcaagg atttcatcgc
     4741 gatccccaag gccaacggct accagtcgct gcacacggtg ctgttcgggc cgttcggcgc
     4801 gccgatcgaa gtccagatcc gcagcgagga catgaacacc gttgccgagc ggggcgtcgc
     4861 ggcgcactgg tcgtacaaga ccgaaaccac cccgcccaac agcgcgcaac tgcgtgcccg
     4921 tgcatgggtc gagacgctcg cggacaacca ggcggccacg ccttcctcgc tggaattcct
     4981 cgaaaacgtc aaggtcgacc tgttccccga cgaggtctac ctgttcaccc cggccggcga
     5041 catccaggcg ctgccgagga acgcgaccgc gctggacctg gcgttcgcgg tgcacaccga
     5101 tgtcgggatg cacgcggtcg cctcgcgcat cgacggcaaa ctcgcttcct tgcgttcgca
     5161 gccatcatcg ggccagaccg tcgaggtcat caccgcgcct tccgccgcgc ccaacccaca
     5221 gtggctggat ttcgcggtca ccggcaaggc gcgtgccgcg atccgccagt acctgaagcg
     5281 gctgcagcac gaggacgcga tcgacttcgg gcaccggatg ctcgaaagcg cgctcgacac
     5341 gcggggctcg agcctcgacg ccgtgctgcc ggaggtgctc gaccggttcc tcgccgaaca
     5401 cagcctgcgc cggctcgagg acctgctctc cgacatcgcg ctgggcaacc gcatgcccga
     5461 ccaggtcgcg gcgaagctga ccgaaatggg cgggccggtg atcggggccg cgctgcatcg
     5521 ttcggagaag atacgcatca gcggcgccga acgcggcatc ctgagtttcg gcaactgttg
     5581 ccacccgctg ccgggcgacg acatcgtggg ctacctctcg ccaggcaagg gcatcgtcgt
     5641 gcatcgggtc gagtgcccga acgtggtcga actggccaag ctccccgaac gccgcgtcga
     5701 gatcggctgg gacaaggagg tgcagggcga ctaccgggcc gaactgcggg tcgaggtcgt
     5761 caaccggccg ggcgtgctgg cgacggtttc cgccgcgatc gccgaagcga attccaacat
     5821 cgacgatgtc aagtacattg agcgcggcat ccaggccgcc accctgatct tcgcgatcga
     5881 ggtgaagaac cgcaagcacc tcgccgacgt gatgcggagg atccgcaaca ccaaggtggt
     5941 caacggggtg taccggtatc cgttgtgacg acgcttttgc cagttacatt ggaaaatctg
     6001 ttggcgtacc ttgcggatgt cctgtttccg gcgcgcaagg cgatggcgga gcttcgcgcg
     6061 cgctgggcga agcccggtga caaggacggg tggcgcgaca gcgattactt cgatctcgtt
     6121 cgcgatcggg aggcgtcgtc gtccatcgac gacaagacct gggtggatct cgaatatccg
     6181 aagatcttcg cctccatgga ctcgacggtc actcccgtgg gaagccaggt gctgtaccgc
     6241 cggatgcgcg agtacagcgg agatgcggca acgctcgcgg cgaactacgc ggtctgcgcc
     6301 gaactgcgtt cgaacgcggc gttgcgggag acgctccagc gcaggctgct gcccctcaag
     6361 gatgaatcca acaatcgcat cgtcgatttc gttttcggga agttgccgcc ggcgccacgc
     6421 caccaggcgc tgctgtggtc atggagcctg gcctcggccg cggtgctggg cggcgtggtc
     6481 tggtgggcgt ggccggcgtg gatatgggtc gccatgctgt tcgtcaacgc gttcctgatc
     6541 ttccgcgcat gcgccgactc gctgcgtgat gcggagacgc tcacgcaatg cctgctgctg
     6601 gtgaaagtgg cggatgacct tgccgcgctg cacgaggccc atcgttcgct tccgcagttg
     6661 gcggggctcc acggggaagc gtcgaaccgc cgggcggtgc gcaaggcgct gcgctggctg
     6721 gctcgcttga agcggcccga gagcgccttc gtgttcgtct ggctcaactt cgccttcctg
     6781 ctggagttgc tggcgcacgg gcgggccatg cgccggttcg ccgtgctcag gcacaagctg
     6841 gccgcatcgt tcgaactggt cggcgaaatc gacgcgatga tcgcgatggc gtcctggctg
     6901 gcatatcgcc ccgaccattg ccatccttca ttcagcgact gcgccatgct ggatatcgag
     6961 gacggcgggc atccactgct ggaagccggt gtccgcaatt ccatccggct ggagcaacgc
     7021 tctgccctgg tcaccggatc gaacatggcc ggcaagacca ccttcatcaa gatggtgggc
     7081 gtcaacgtgc tgctggggca gacgctcggc ttctgcctcg ctgcgagggc cacgatcccg
     7141 cacgccccgg tcatggcttc gatccaggcg gggcattcgg tcgcgtccgg caagagccat
     7201 tacttctccg aagtcgagac gattcgttcc ttttttgccc ggcaggcgga ccaacgccgc
     7261 gccatcttcg tgatcgacga attgttcacc ggcaccaata cgaccgaacg cgtcgcggtc
     7321 gcacgcgcgg tgctggaatc gctgggcgaa ggctcgatcg tgctggccac gacccacgac
     7381 gtggaactgc aggcgctcct ggacgaacgc tacgatctgt accacttcca ggagagcccc
     7441 gacgtggacg gattcttcga ctaccgcctg cgagcgggcc ctgccagcga gcgcaatgcg
     7501 atccggctgc tcggcgaact gggcttcccc gccaggatcg tcgcgaaggc gatgagatac
     7561 gcccgcgagg agttggccgg cgagaagcgg ggcgccgacg tgccgcgccg cggcgtccgg
     7621 accaaaccgg tcggctccgc ctgaacccgc ggcggctcgc gcggccggtg cgtcttgcgc
     7681 ggccggggta aactgcacgt ttcgccaccg cgagcccgcg catgccacgc caaccgatcc
     7741 attccgattc cgcgcccaag gccatcggcc cgtattcgca agccgtgcgt tcgggcaaca
     7801 ccttgtacct ttccggccag acgccgctcg accccgccac gggcgaaatg gtcgcgggcg
     7861 ggatcgacga gcaggccgag cgcgtgttcg agaacctgaa ggccgtgctg ggcgcggcga
     7921 atgcgacatt cgacgacgtg gttcgcgttg ccatctacat gaccgacctc ggcaattttt
     7981 ccgcggtcaa cgaggtcatg aagcgctact tcaacgaacc atatcccgcg cgctcgacca
     8041 tcggcgtggc cgcgctgccg cgtggcgcgg cggtcgagat cgatgtaatt gcagccgtcg
     8101 atcgctgagt accgccgcgg tcgcaccccg gggcccgcag cccctgcctc agcgcatctg
     8161 cgtaaacagc acaccggtat aggttgccat gttcaggagc gccagcaatg ccacgcaggc
     8221 ctgcacgcga gtctgccgcc acaggaagcc cggcacggca atggccagaa acacgagtcg
     8281 gaagccggtg tccaggcttg ccgagggcag gtggccggtg agcaggcgct cctgcaggaa
     8341 gctggcaagt ggcaccagcg caaggcaggt gtagaagggt ttgcggtggt cgacgaattc
     8401 gcgcgcaagt tcgtgccccg tcaggtgcag gttgtcaggc agcagcaggt aggccagcag
     8461 gtacatcagc accggaagga tcatgaatgc tgcgtaggca aggaaggtcc actcctcaac
     8521 ccggcgccac tcgtgggaaa cccaccagat ctgcagattt gccaaaagca gcatggcgat
     8581 gaggacgaag atccaccacg ctggtgtcag cgagcgtccg tcgcgcaata ggcgagcaaa
     8641 acctgccagc agctgggtca cggccaggcc gaggatgatc gacagcaata cggtgatgta
     8701 ttcaaattcg ctcatcggaa atgccggaga cggaatccgc gcatggtagc cgtggagccg
     8761 gaaagccgcg caccgcatgg catccactaa tcttgcgcgg gtgacttccc ccgccgacac
     8821 gcgcatcgaa cgctcgcccg atcccggact cgcgccggcg acgctgctgc ccggcgtcgg
     8881 cccggcgctc gcccaaacgc tgcgca
//
LOCUS       DASSNH010000004        16334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_373338, whole genome shotgun sequence.
ACCESSION   DASSNH010000004 DASSNH010000000
VERSION     DASSNH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373338"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2447
                     /locus_tag="VFK00_00125"
     CDS             <1..2447
                     /locus_tag="VFK00_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7591395.1"
                     /translation="DFYNRNGFSEAPFLFYQEFQVKTGGYSVEYGRTTGGVVNAVVRS
                     GSNQFHAGAQYTFEPRDLQASKRDLYDADGRYLTSSRDNYSLNKLDLYASGPIIKDKL
                     FFFAMYELRDYRPQNTNNAGTVLTTNKNGTGFWGGTIDWRITDNNTLSLMAFSDKNHE
                     IGNVYDYDFDADSKGQQTNTVYNDTGGKSGNLTWTSFLTDNFTMKLMWGRNQREAFSH
                     SLRDDECNYVSADSDVPQPDLPLGCTTNSTVISRDDVRHQDRADFEWTLGNHLLKFGY
                     DHEKNVSNYSRYYTGPGAYYYNVYATTPGATISNGGVVPPGYFAYIRQRQYEISGTFA
                     TIDSAYYIQDDWQVTPNLLLSLGLRNDSFDNQDAAGRSYIKIDNQPAPRFGFSWDMKG
                     DGTTKLFGNLGRYYLPVANVINIKQAGGLLDSRTYFAFDGWETQELNGSQYAVPILGP
                     QIGQVDDSQGDGTVGDLRAEVDKNMDPVYQDELILGFEQALDQTWAWGVKGTYRRLHN
                     AIDDMEISATAACGGDGYIGWVMANPGQPVTVWGDTDCDGTADGYVTVDTSKEGWAMY
                     DNEGNYLGQTGWSKPKRKYYALEFDVDRAWDGKWSMNASYTISWTRGNAEGPVNSLTD
                     FDDTGRTENFDNPWVNYAGYGPLSNARRHQFKFRGAYAINDHWRLGAVADIHSGAPIS
                     GLGSGNPFDDTNYYSYFICVDKCGNIPGQFDADGDPVPYPSEDRVYVHSPRGGYGHTP
                     WIFDLNASVSYLTKIKDANLQVKFAVYNLLNQQRTTVVDEQLQGDISDETSPTWGQTI
                     GFSSPRFFQLTVSVDF"
     gene            2507..3292
                     /locus_tag="VFK00_00130"
     CDS             2507..3292
                     /locus_tag="VFK00_00130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7591396.1"
                     /translation="MPRRRRTHHPQAAKLLCTGLMLALLAACAPLPPRNPIATWVPSK
                     NRDIRAPQLIVLHFTDQDSVQQSLDTLRTANSGGPVSAHYLIGRDGRIYQLVSDVDRA
                     WHAGGGRWGTITDVNSASIGIEIDNNGQAPFPPQQIASLINLLTDLTTRWGIPRAQII
                     GHEDMAPARKDDPGPMFPWAALAAAGFGLWPDPPATLPDPPRGFDPWMALTAIGYSLD
                     DPPAAVRAFHDHFRGMRGDTLDAEDLRILYNLVGKIERGATAK"
     gene            4025..4618
                     /locus_tag="VFK00_00135"
     CDS             4025..4618
                     /locus_tag="VFK00_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591397.1"
                     /translation="MDKRKAFTRKDFLIKDLAISIGGGARSGTWMPGPDDPTPPTPIS
                     PIASVLANIELIESVRGAILEAAKNQQFDEVAGAFVAGSSAGNAIIRNAIHDIGAAVV
                     ASAAYSALGSSVGLVNPECGGTSMETIPPTLTPIVHVGFSVHRVSELPRLKRQLADAI
                     SFIDKAAEAQAPRGAEVAMVRTQLENVLKKETATMAT"
     gene            4646..5383
                     /locus_tag="VFK00_00140"
     CDS             4646..5383
                     /locus_tag="VFK00_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591398.1"
                     /translation="MSRAALEMAARDQPSEPAPLARWLYRYGSLPRTAAHERDFGPDD
                     EPLGVLGLAVGGAVRRRLEETYEASTLPGWYSFARAGQPTQWIAACKLYVSPLPEALP
                     NAFAHIVDAFITADVRSFKVGRGIEGLLRPDKLIAYFDDPNHMTDAAHALQRRLRGCP
                     AQGVPFTAELGGDGLMSSGVDPPLGGATASWRAWITARLAHSLVAKRCRAPADTVAAV
                     LGDLRAAGIDTARWRPEPGTFTQPTPP"
     gene            5380..7506
                     /locus_tag="VFK00_00145"
     CDS             5380..7506
                     /locus_tag="VFK00_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7591399.1"
                     /translation="MSASTPDFAQTRFVLPPDAKVLPVAELSARARTRIGALADGQSV
                     VTRHGFRVTTRLIPPPLAELLGEFRTPSRITDGVLRFALARGQDPQALLDVAFDALAT
                     MVEARFLVPEDASDAAAPGASLAAGQDFAGFEIDAAVRSLDDSEVFQARAQDGKAVAL
                     KIARDGRQEVHAVLAHEGQILQRLGGRDSPTLYAQGVHAGRPFLAMEWCSGVSIGMAA
                     QRLRAARERPGLHALVVRLLQAYGRLHGCGVLHGDIHPGNCVVRDDGRIVILDFGHAR
                     LIDDMPGGVDLARTGIPQFHDPQMARALLDGVVTPAASPASEQYAIAALAYLLLTGLH
                     PIDAPAVRDELLRRIAVRAPLPFAARGVPAWPAVEQVLRRAMSQEPGQRFADVQVMAR
                     AFAAADAAADFGWRAPPRGPSERAFIAALQGVRRLVPMQRFQRDQAWFALRAALALED
                     AELLAAAELLSGRAGRGWAACTVAAAVAQARSDFRAEGRAITSFLAATEGLTAGLPQA
                     RMLLAASRILEGTSPRCTESVALSSWCAQRVDDLLSVDRYATSGQAVAEPLYLLAALA
                     VVRTGRVPMHPALESSLEGPARTGRGGVWLWALAHDVFAEDRFRTLALSARLPDAPLM
                     RSFALLRRYQLTSEPRFFLVARRTARAASSGLPDAATALLVAELHAPETALPPPFPGI
                     FGVPLRCAPAPRHAIMPVPVTAAAAT"
     gene            complement(7540..9258)
                     /locus_tag="VFK00_00150"
     CDS             complement(7540..9258)
                     /locus_tag="VFK00_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7591400.1"
                     /translation="MSSTTIQSAGDDHRLKRHIGTVGLLFTAVGSIIGSGWLFGAMNA
                     AQQAGPASILSWLVGAVMILFIGLTYAELGTMFPVSGGVVRFPHFAFGSFASYVSGWI
                     TWIATVAVPPLEVLAVLQYAANYIPWLQTLKGGSPVLTTPGLGVACALLLLFVLINML
                     GVRWFARINNTLVVWKVGIIIIAIVAFLSVQFHPDRFFAPRLGGFMPMGWHGVFSAVA
                     TAGVVFSYLGFRQGVELAGETSNPQRNVPIAILGSLALCTIIYLGLQVAFIGALPDSA
                     LANGWANIGTSFVAGIGEHSMAFGPLAALAAALGLTWLAILLYVDAFLSPSDTGLIYT
                     ILSARLTYAMGRNGNAPKALSHVNAKGVPWISVVLAFFVGLLFFLPFPGWQKLVGFHT
                     SAMVMSFGSGPLAMMALRRELPDHPRPFRLGGGWVIPYLAFLSSNLIVFWSGWDVVWK
                     LMVAVLLGFVLLAIHETGWGKHTPKLEMRAGFWMLPWLGGVTLISWLGRYPALDKHAG
                     NLGWLDMVSGVVVIVIFSALIMGLAHALRLRGQAVVGQLETHKARVADHPSSPARSQA
                     AGELPD"
     gene            complement(9492..10745)
                     /locus_tag="VFK00_00155"
                     /pseudo
     CDS             complement(9492..10745)
                     /locus_tag="VFK00_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
     gene            complement(10927..12735)
                     /locus_tag="VFK00_00160"
     CDS             complement(10927..12735)
                     /locus_tag="VFK00_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7591401.1"
                     /translation="MKSFSRLLPGVAIIVVATAFASSAPIGGFPPTLQPADLVLRDGT
                     IATLDPLQPNAQALAVRDGKILAVGSDASISHYIGKQTRVMDLHGAFVTPGFVEGHGH
                     LMGTGEALMQVDVGKAANWDEIVAIVKAAVAKAKPGQWIIGQGWQQAKWNKVPQPNVD
                     GLPLPDGLDAVSPDNPVLLNHASGHGIYANALALKLAGITDHTPDPSGGSIVRDSKGR
                     AIGMLRDTAANPVFAAYNHHIQSLPPAAREARREKALELAVQNEISKGITTFVDQGED
                     FETIDWLKKQAAKGLPLRLYVYVDGESVASLDKFLAAHRTIGFADNHFTVRGIGETVS
                     DGALGTRSAWLLEPYSDAPNITGKNVTPMADLTQMSEIAAREGFQMAIHAIGDRANRE
                     VLDMYQTIFEKDPAAHALRWRIEHAQHLNPADIPRFAELGVIASMQSIHACSDAPMVV
                     ARLGEQRAEEGAYVWKKLIDSGAIVLDGTDTPVEDTNPIPNFYCGVTRAYDHGRKTFF
                     PDEAKTRMQELKSYTWNNAYAIYQEHELGSLAPGKLADMDVFSDNLLTMPAGDILRTR
                     VLYTIVGGKIVYRRSDAGKWRKGQQFDAMPEFNHAE"
     gene            complement(12881..13807)
                     /locus_tag="VFK00_00165"
     CDS             complement(12881..13807)
                     /locus_tag="VFK00_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7591402.1"
                     /translation="MRLRYIELFHAVLTTGSLTGAAKLLNISQPAASKALQHAEDKLG
                     FALFSRVRGRLQPTQQAFLLRDRIEKIIQDVHELQRLTTNMSQPGSCPLRVTCTPTLA
                     LTLLPAATVLLRKTFPDAIAELSTQHSAAMCESLTLRETDIGLTLQDTGQQNLRQKVL
                     CRGQVMVIAPPGWWSDSELAQPLPIGALADQPMIGITVRDALGSMLQSHLAQVDPAPR
                     TTVSVQTYQLAHALVAKGEGLALVDPFTARCGSAGSVQMRPLKPHLDVILYAMHRPDS
                     PLDPVQKRFLDLVRQQAQRLLAQRPDAAGGAS"
     gene            13834..15024
                     /locus_tag="VFK00_00170"
     CDS             13834..15024
                     /locus_tag="VFK00_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7591403.1"
                     /translation="MSFRIVIAAALLLGSLPAGARHVDLRAYGIAIGVLPTGKWNAVT
                     DVPGVRVGQKTLVAGDDVRSGVTVILPHPGNVFQSKVPAAVYVGNGFGKLTGTTQVAE
                     LGNIETPIVLTNTLSVPTAADALIDYTFSFPANAEVRSVNPVVGETNDGFLNDIRGRH
                     VTRQDVLDAIHAATGGKVAQGNVGAGTGTVAFGFKGGIGTSSRQLPASLGGYTVGVLV
                     QTNFGGVLNINGVPVGKLLHHYYLGDKLDRAPDPSPDGSCMIVVATDAPLGSRNLGRL
                     AKRAILGLAKTGGIESNGSGDYVVAFSTSEAVRVPYESKTPLQPMTVVRNDDMSPLFM
                     GAIEATEEAILNSLFHAETMTGRDHHTVAALPVDKVLRIMQQRGYPVVMAPGDAPAEA
                     TSAR"
     gene            15170..16201
                     /gene="dgcN"
                     /locus_tag="VFK00_00175"
     CDS             15170..16201
                     /gene="dgcN"
                     /locus_tag="VFK00_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN"
                     /protein_id="HET7591404.1"
                     /translation="MRGEATGRWGPPYLLYLGTAADDLAIKTARGLAYWRPDWCVGQH
                     RNPACNLTLGLPDLGFADARRAGADTMVIGTANAGGVLAPETVADIIAALDAGLNVIS
                     GLHQKLRDNPDIVAAATRNRRVLFDAREFNRNIPVGNGRKRAGRRLLTVGTDCSVGKM
                     YTTLAIERSMHKRGLVADFRATGQTGIFVAGGGIPVDSVIADFISGAAEWLSPAREDG
                     GWDLIEGQGSLFHPSYAGVSLGLLHGSQPDALVLCHEPTRKHMRGLPDSPIPDLERCL
                     EANVVAARLTNPAAKPVGVALNTSQLSGEQAQAACREISDRLGLPCEDPVTMGVEAIV
                     DNLLACCAN"
BASE COUNT         2843 a         5609 c         5210 g         2672 t
ORIGIN      
        1 ccgacttcta caaccgcaac gggttctccg aggcgccctt cctgttctat caggaattcc
       61 aggtcaagac cgggggctat tcggtcgagt acggccgcac caccggcggc gtggtcaacg
      121 ccgtcgtgcg cagcggcagc aaccagttcc acgcgggcgc ccagtacacc ttcgagccgc
      181 gcgacctgca ggcgagcaag cgggacctct acgatgccga cgggcgctac ttgacgtcca
      241 gccgcgacaa ctactcgctc aacaagctcg acctctacgc gtccggaccg atcatcaagg
      301 acaagctgtt cttcttcgcg atgtacgagc tccgcgatta ccggccccag aacaccaaca
      361 acgccggcac cgtactcacc accaacaaga acggcacggg attctggggg ggaacgatcg
      421 actggcgcat caccgacaac aacaccctca gcctgatggc gttctcggac aagaaccacg
      481 aaatcggcaa cgtctacgac tacgatttcg acgccgacag caagggccag cagaccaaca
      541 ccgtctacaa cgacaccggc ggcaagagcg gcaacctcac ctggaccagc ttcctcacgg
      601 acaacttcac gatgaagctg atgtggggcc gcaaccagcg cgaggcgttt tcgcattctc
      661 tgcgcgacga cgagtgcaat tacgtgagcg ccgacagcga cgtaccgcag cccgatctcc
      721 cgctgggctg caccaccaat tccaccgtga tctcgcgcga cgacgtccgc caccaggacc
      781 gcgccgactt cgagtggacc ctcggcaacc acctgctgaa gttcggctac gaccacgaaa
      841 agaatgtctc caactattcg cgttactaca ccggccccgg cgcctactac tacaacgtgt
      901 atgccaccac gccaggagcg acgatcagca acggtggtgt agtgccgccg ggctacttcg
      961 cctacatccg ccagcgtcaa tacgaaattt ccggcacctt cgccaccatc gattcggcgt
     1021 attacatcca ggacgactgg caggtcaccc cgaacctgct cttgagcctc ggcttgcgca
     1081 acgacagctt cgacaaccag gacgccgctg gacgcagcta catcaagatc gacaaccagc
     1141 cggcgccgcg cttcggattc tcgtgggaca tgaagggcga cggcaccacc aagctgttcg
     1201 gtaacctcgg ccgctactac ctgccggtcg ccaacgtgat caacatcaag caggccggcg
     1261 gactgctgga ttcacgcacc tacttcgcgt tcgatggctg ggagacccag gaactcaatg
     1321 gttcgcagta cgcggtcccg atcctcgggc cgcagatcgg ccaggtcgac gactctcaag
     1381 gtgacggcac cgtgggcgac ctgcgcgcgg aagtcgacaa gaacatggac ccggtgtacc
     1441 aggacgaatt gatcctgggc ttcgaacagg ccctcgacca gacctgggcc tggggcgtca
     1501 agggcaccta ccggcgcctg cacaacgcga tcgacgacat ggaaatcagc gctactgccg
     1561 cctgcggcgg cgacggctac atcggctggg taatggccaa tcccggccag ccggtgacgg
     1621 tctggggtga caccgactgc gacggcacgg cggatgggta cgtcaccgtc gacacctcca
     1681 aggaaggctg ggcgatgtac gacaacgagg gcaattacct cggccagacc ggctggtcga
     1741 aacccaagcg caagtactac gcgctcgagt tcgacgtcga tcgcgcctgg gacggcaagt
     1801 ggtcgatgaa cgcctcgtac acgatttcgt ggacgcgcgg caacgccgaa ggtccggtga
     1861 actcgttgac cgatttcgac gataccggcc gcaccgagaa cttcgacaat ccgtgggtga
     1921 actacgccgg ctacggccca ctgtccaacg cccggcgcca tcagttcaag ttccgcggtg
     1981 cctacgccat caatgaccat tggcgcctgg gcgcggtcgc cgacatccac tccggcgcac
     2041 cgatcagcgg gctcggttcc ggcaacccgt tcgacgacac caactactac agctacttca
     2101 tctgcgtcga caagtgcgga aacatcccgg ggcagttcga cgccgacggc gacccggtcc
     2161 cgtatccttc cgaagaccgc gtgtacgtcc attcgccgcg tggcggctat ggccataccc
     2221 cgtggatctt cgacctgaat gccagcgtca gctacctgac aaagatcaag gacgccaacc
     2281 tccaggtgaa gttcgcggtc tacaacctgc tgaaccagca gcggacgacg gtggtcgacg
     2341 agcagttgca gggcgacatt tccgacgaga ccagccccac ttggggccag acgatcggct
     2401 tctcatcgcc gcgtttcttc cagttgaccg tcagcgtcga tttctgatcg tggatttgcg
     2461 ccaagccggc gggatgccgg cgcgctgcag cgaggaagcc gtgatgatgc cgcgccgacg
     2521 gcgcacgcat cacccacagg ccgcgaagct gctttgtacc gggctcatgc tggcgctgct
     2581 ggccgcctgc gcgcccctgc cgccgcgcaa ccccatcgcg acgtgggtgc cgtcgaagaa
     2641 ccgcgacatc cgcgcgccgc agttgatcgt gctgcacttc accgaccagg attcggtgca
     2701 gcaatcgctg gacacgctgc gcaccgccaa cagcggcgga ccggtcagtg cgcattacct
     2761 gatcggccgc gacggccgca tctaccaatt ggtcagcgac gtcgatcgcg cctggcatgc
     2821 cggcggcggc cgctggggca cgatcaccga cgtcaactcc gcctccatcg gcatcgaaat
     2881 cgacaacaac ggccaggctc cgtttccgcc gcagcagatc gccagcctga tcaacctgct
     2941 gaccgacctc accacccgct ggggcattcc tcgcgcacag atcatcggcc acgaggacat
     3001 ggcgcccgcc cgcaaggacg atccgggccc gatgttcccg tgggccgcac tggcggcggc
     3061 agggttcggc ctgtggcccg acccgcctgc cacgctgccc gatccgccgc gcggcttcga
     3121 cccgtggatg gccctgaccg cgatcggcta ttcgctggac gatcccccgg ccgccgtccg
     3181 ggccttccac gaccacttcc gcggcatgcg cggcgacacc ctggatgcag aggacctgcg
     3241 catcctctac aacctcgtcg gcaagatcga gcgcggtgcc accgcgaaat agcccggcac
     3301 ctggcccggg gtgccaggtg tcgcgggtgg tgaccaggct gtcgaggaca tccccgccag
     3361 ccgatggata ttgttctggt tccccccact cgccgacgga gagaagtttt gggcgtccgg
     3421 tcctcgtgtt catcgaggta ccgaaggcga gcaaaaaaac ccaggaaaaa ggacgcgttc
     3481 cgcctcttgt tattttgcct caaggggcgt acaaaacgca ttcggagcaa agtcgctact
     3541 gaaagcggct ctgccaaaga gtggctctca tggcatcgcc tgaccaccgt ggtcggttcg
     3601 catcgatccg cgcgcaacag gtggatcgaa tcgtcgggca cagcatgcgc cacccaccgt
     3661 cacggccccg gtttggccgc cgcgtccctc gatggcagcc gaacgttctg gcgtggagtt
     3721 ccggggatca cgaagaactt cgccagatcc ggcactccct tcttctagtt ccttttttgc
     3781 gcgtgcctgt gaccggcccg ggtttgacgc ctggccggcg cgcgcaaatt cctgcgccgg
     3841 gtgttccggc aacggcggcc ccacgaggcc ggctgaaagg caacgatgga caacaacacg
     3901 tactcaaagc acatcttcat ccgcttctcc ttggggggtt gatcgcaaca tgttggccgt
     3961 caccgcgagt ccgcggctcg gcgaaagtcc cgcgtgacga gcgggcgcca caaaggagga
     4021 tgtgatggac aagcgtaaag cgtttacacg caaggacttc ctgatcaagg accttgctat
     4081 cagcatcggc ggtggagcgc gcagcggtac ctggatgccc ggacccgacg acccgacgcc
     4141 accgacgccg atttccccga tcgcgagcgt gctggccaac atcgaactga tcgaatcggt
     4201 acgcggcgcg attctcgaag ccgcgaaaaa ccagcagttc gacgaagtgg ccggggcctt
     4261 cgtggccggc agcagtgcag gcaacgccat catccgcaac gcgatccacg acatcggcgc
     4321 ggccgtggtg gcgagcgcgg cctattcggc gctgggcagt tcggttggcc tggtgaaccc
     4381 ggaatgcggt ggcacctcca tggagacgat accgcccacg ctgaccccga tcgtgcacgt
     4441 cggcttctcg gtgcaccggg tcagcgaact gccacggctg aagcggcagt tggcggatgc
     4501 gatatcgttc atcgacaagg ccgccgaggc ccaggctccg cggggcgccg aagtagccat
     4561 ggtgcgaacg cagctcgaga atgtgctgaa gaaagagact gccacgatgg cgacatagca
     4621 tcgtgtgagg gaccgaatcg cccggatgtc acgggccgcc ctcgagatgg cggcccgtga
     4681 ccagccgtcc gaaccagccc cgctggcgcg ctggctgtac cgctatggca gcctgccacg
     4741 cacggcggcg cacgagcgtg acttcggacc cgacgacgag cccttgggcg tgcttggcct
     4801 cgcggtcggc ggtgccgtcc ggcgccgcct cgaagaaacc tacgaggcaa gtacgctgcc
     4861 cggctggtac tccttcgccc gcgccgggca gccgacgcaa tggatcgccg cctgcaagct
     4921 ttacgtgagt ccgctgcccg aggcgctgcc gaatgccttc gcgcacatcg tcgatgcctt
     4981 catcacggcc gacgttcgtt ccttcaaggt cggtcgcggc atcgaaggat tgctgcgtcc
     5041 cgacaagctc attgcctact tcgacgaccc caaccatatg acggacgccg cgcacgcctt
     5101 gcagcgccgg ttgcgaggtt gtccggcgca gggcgtgccg ttcaccgccg aactcggcgg
     5161 cgacgggctg atgtccagcg gcgttgaccc accgctgggc ggagcgactg caagctggcg
     5221 cgcgtggatc acggcgcgcc tcgcccactc cctggtcgcg aaacgctgtc gcgcgccggc
     5281 cgacacggtg gcggcggtgc tgggcgacct gcgtgccgcc ggcatcgaca ccgcacgatg
     5341 gcggccggaa cccggcacgt tcacccagcc gacgccgccg tgagcgcatc gacccccgat
     5401 ttcgcgcaaa cgcgtttcgt cctgccgccg gacgccaagg tgttgccggt ggccgagctg
     5461 tcggcccgcg cgcggacgcg tatcggtgcg ttggccgatg gccagtcggt cgtcacccgg
     5521 cacggctttc gcgtcaccac ccgcctgatc ccaccgccac tcgccgaatt gctcggcgaa
     5581 ttccgcacgc ccagcaggat caccgacggc gtgctgcgct ttgcccttgc ccggggccag
     5641 gatccgcagg ccctgctcga cgtggccttc gatgccttgg ccacgatggt cgaggcgcgt
     5701 ttcctggtgc cggaggatgc atccgacgct gctgcgcctg gagcgagcct tgcagccgga
     5761 caggatttcg cgggcttcga gatcgatgca gccgtgcgat cgctggacga ttccgaagtc
     5821 ttccaggcgc gcgcgcaaga cgggaaggcg gtggcgctga agattgcccg cgatggccga
     5881 caggaagtcc atgcggttct cgcgcacgag gggcagatcc tgcagcggct tggcggccgg
     5941 gacagtccga ccctgtacgc ccagggcgtg catgccgggc gcccgttcct ggccatggag
     6001 tggtgcagcg gcgtgtcgat cggcatggcg gcacagaggt tgcgcgctgc gcgcgagcgg
     6061 ccgggcctgc atgcgctggt ggtgcgactg ttgcaggcct atggccgcct gcacggttgc
     6121 ggcgtcctgc acggcgacat tcatcccggc aactgcgtcg tgcgagacga cggccgcatc
     6181 gtgattctcg acttcggcca tgcgcgcctg atcgacgata tgccaggcgg tgtcgatctc
     6241 gcccgtaccg gcattccgca attccacgat ccccagatgg cgcgcgcctt gctggacggc
     6301 gtggtcacgc cggcggccag cccggcatcg gagcagtacg ccatcgccgc actggcctac
     6361 cttctgttga ccggcctgca tccgatcgat gcacccgccg tccgcgatga attgctccgt
     6421 cgaatcgccg tgcgggctcc attgccgttt gcggcgcgcg gggtgccggc gtggccggcg
     6481 gtggaacagg tgttgcgccg cgcgatgtcg caagagcccg ggcaacggtt cgcggacgtc
     6541 caagttatgg cacgcgcgtt cgcggcggcc gatgctgccg cggatttcgg ctggcgggca
     6601 ccgccgcgcg gtccgtccga acgggcattc atcgcggcgt tgcagggagt gcggcgcctg
     6661 gtgccgatgc aacgcttcca gcgcgaccag gcgtggttcg ccctgcgtgc ggcactggcg
     6721 ttggaagatg cggaattact cgcggcagcc gagttgctct cgggccgcgc gggccggggt
     6781 tgggccgcct gcacggtggc agccgcggtc gcgcaagcgc gttcggattt tcgcgccgaa
     6841 ggcagggcaa tcacctcgtt cctggctgcg acggaaggct taaccgcggg attgccgcag
     6901 gcaaggatgc tgctcgcggc ctcacgcatc ctcgagggca cgtcgccgcg ctgcaccgaa
     6961 tccgtggcgc tctcctcatg gtgcgcacaa cgggtggatg acctgctgtc ggtcgaccgg
     7021 tacgcgacct cggggcaagc cgtcgccgaa ccgctctacc tgttggccgc tcttgcggtg
     7081 gtcaggaccg gccgcgtgcc gatgcacccc gcgctggaat cctccctgga aggcccggcc
     7141 cgtacgggtc ggggcggcgt gtggctatgg gccctggcgc acgacgtgtt cgccgaagac
     7201 cgcttccgga cgctcgcgtt gtccgcgagg ctacccgacg cgccgttgat gcggagcttc
     7261 gcattgctgc gccgctatca gctcaccagc gaaccccggt tcttccttgt agcccgacgc
     7321 acagcacgcg ccgcctcgtc cggattgccg gatgcagcga ccgccctgct ggtcgccgaa
     7381 ctgcatgcgc cggaaaccgc gctgcccccg ccgttccccg gcatcttcgg cgttccgttg
     7441 cgctgcgcgc cagcgccgcg gcatgcaatc atgcccgttc cagtcaccgc cgctgccgcg
     7501 acatgaccca ttcagaatcg tttgcgtctt ccggcgcagt cagtccggga gctcgccggc
     7561 agcttgggat cgcgccggcg acgatgggtg atcggcaaca cgcgccttgt gcgtctcgag
     7621 ttgtccgacc accgcctggc cacgcaaccg cagcgcgtgc gccaatccca tgatcagcgc
     7681 ggaaaaaatc acgatcacca ccacgccgga aaccatgtcg agccagccga ggttgccggc
     7741 gtgcttgtcg agggcgggat agcgccccag ccaactgatc agcgtgacgc cgcccagcca
     7801 cggcagcatc cagaatcccg cacgcatttc gagcttcggc gtgtgttttc cccagccggt
     7861 ttcgtggatc gccagcagca cgaagcccag cagtaccgcc accatcaatt tccacaccac
     7921 gtcccagccc gaccagaaca cgatcaggtt ggacgacagg aacgcgaggt acgggatgac
     7981 ccagccgccg cccaaccgga acggccgcgg gtggtccggc agttcgcggc gcagcgccat
     8041 catcgccagc ggccccgagc cgaacgacat caccatcgcc gaggtgtgga agccgacgag
     8101 cttctgccag cccgggaacg gcaggaagaa cagcagtccg acgaagaacg ccagcaccac
     8161 gctgatccac ggcacgccct ttgcgttgac gtgcgacaag gccttgggcg cgttgccgtt
     8221 gcggcccatc gcgtaggtca ggcgcgcgga caggatcgtg tagatcagcc cggtgtccga
     8281 cggcgacagg aacgcatcga cgtacaacag gatcgcgagc caggtgagcc cgagcgccgc
     8341 ggccagcgcc gccaacggcc cgaacgccat cgaatgctcg ccgatgccgg cgacgaagct
     8401 ggtgccgatg ttggcccaac cgttcgccag cgccgaatcc ggcagcgcac cgatgaatgc
     8461 cacctgcagg cccagataga tgatcgtgca cagggcgagc gagcccagga tcgcgatcgg
     8521 cacgttgcgc tgcgggttgc tggtctcgcc ggcgagttcc acgccctgcc ggaacccgag
     8581 gtacgaaaac accacgcccg ccgtcgcgac cgccgagaac acgccgtgcc atcccatggg
     8641 catgaaacca ccgagtcgcg gtgcgaagaa ccggtcgggg tggaactgca ccgagaggaa
     8701 cgcgacgatc gcgatgatga tgatgccgac cttccacacc accagcgtgt tgttgatgcg
     8761 cgcaaaccag cgcacgccca gcatgttgat gagcacgaac agcaacagca gcgcgcaggc
     8821 gaccccgagt cccggcgtgg tcagtaccgg cgagccaccc ttcagggtct gcagccacgg
     8881 aatgtagttg gccgcgtatt gcagcaccgc caggacttcc agcggcggga ccgccacggt
     8941 cgcgatccag gtgatccagc ctgagacata gctcgcgaac gaaccgaacg cgaagtgcgg
     9001 gaagcgcacg accccgcccg acaccgggaa catcgtcccg agctcggcgt aggtgaggcc
     9061 gatgaagagg atcatcaccg cgcccaccag ccaggacagg atcgacgccg gcccggcctg
     9121 ctgggcggcg ttcatcgcgc cgaacaacca gcccgacccg atgatcgacc caacggccgt
     9181 gaacaacagg cccaccgtcc cgatgtggcg cttcaggcga tggtcgtcgc cggcggattg
     9241 aatcgttgtc gacgacatcg tcttcccttc cctcgtcaag cgtgaataac gcaactgatt
     9301 tttccttctc cctccgggag aagggcgcag gacgcgctgc tcttggtttt gcgcgcagcg
     9361 ccggatgagg gtacgagcgt gcgactggca gaatccttgc gagtcgctgc catttcgcgt
     9421 atccgtaccc tcacccgccc cttcggggcg ccctctcccg gagggagagg cgaactttgc
     9481 aaccgcttgc ggtggttgta ccgcgaaata tccagcccct cgctgctgat ctgcggcgcc
     9541 atgcccgcca tcaggtcggc gaggtagcgc cccgacccgc acgccatcgt ccagcccagg
     9601 gtgccgtggc cggtgttgag gtacaggttc cgcaagcggg tggcgccgac cacgggcgtg
     9661 ccgtccgggg tggccgggcg caggccggcc cagaactcac ccttcgccgg atcgccaccc
     9721 ttcgggtaca ggccggcgag caccttctcc agggtcgcgc gccggcgcgt cggcttcgac
     9781 aggtcgtagc cacacagctc ggcggtgccg cctatgcgaa tgcggcggtc gaagcgcgtc
     9841 acggcgacct tccaggtttc gtcgagaacc gtggagacgg gggccatcgc gggattcgtg
     9901 accggcaccg tcagcgaaaa gcccttcaag gggtagacag gaagacgaat ccccagcggc
     9961 gcgagcaacc gcggcgtcca gcttccgagc gcaaggacat agcggtccgc ccgctcgagc
    10021 gtgccgtcga tgcgtgcgcc ggtcacctcg ctgccatcgg tctcgaggcc ttccagcgtc
    10081 gcttggcagc ggaactccac cccggccgct tttgcacgct cggccagcag gcgcgtgaac
    10141 aactcgcagt caccggtctg gtcgttgggc aggcgcaacg caccggtgaa ccggtcgacg
    10201 acatccgcga gcgcgggctc cacccccacg atgccggccc ggtccagcag ctcgaacgga
    10261 acgccgtatt tttccagcac cgcgatgtcg ccggcggccg catccagttg cgactgggtg
    10321 cgaaacagtt gcagcgtgcc gagcttgcgg ccttcgtaat cgatgcgaag ttcgccggcg
    10381 agttcgtcga ggcattcggc gctgtagcgg gcgagccgca gcatccgcgc cttgttgaca
    10441 gcgtagcgcg aggcggtgca gttgcgcagc atccgcaaca accagagata catgcggggg
    10501 tcgagacccg gccggatcac cagcggcgcg tgtcgcgaaa acagccactt caatgccttc
    10561 agcggcacgc cgggcgcggc ccatggcgac gcgtacccgg gcgaaatctg gccggcgttg
    10621 gcgaagctgg cgccgcgggc ggcaccggcc gcgcgatcca ccaccgtcac ctcgaatccc
    10681 gcgcgcgcga gataccacgc gctgcatgtg ccgatcacgc cgctgccgca aacaagtaca
    10741 cgcatcgccc tttcacgccc cgacagcgat caacacaaaa gcctcgacgc ggatgaacgc
    10801 ggataaagcg caacatcctt ccaaaatcaa tgtctcgaat gcaaaacaaa tggctctatc
    10861 cgcgtccatc cgcgtcagaa gctctttgtc accacttcgg gaaagcccaa tcctgtgccg
    10921 cctcgctcac tcggcgtggt tgaactccgg catcgcgtcg aactgctggc ccttgcgcca
    10981 cttcccggca tccgaccgtc gatagacgat cttgccgccg acgatggtgt acagcacccg
    11041 cgtgcgcagg atgtccccgg cgggcattgt cagcaggttg tccgagaaga catccatgtc
    11101 ggccagcttg ccgggcgcga gcgaacccaa ttcgtgctcc tggtaaatcg cgtaggcgtt
    11161 gttccaggtg taggacttca attcctgcat ccgcgtcttg gcctcgtccg ggaagaaggt
    11221 cttcctgccg tggtcgtagg cgcgggtgac gccgcagtag aagttcggga tcggattggt
    11281 gtcttcgacc ggcgtgtcgg tgccatccag cacgatcgcg ccgctgtcga tcagcttctt
    11341 ccagacgtac gcgccttctt ccgcacgctg ttcgccaagg cgcgccacca ccatcggcgc
    11401 gtccgagcag gcgtggatgg attgcatgga ggcgatcacg cccaattcgg cgaaacgcgg
    11461 aatgtcggcc gggttcaggt gctgcgcgtg ttcgatgcgc cagcgcagcg cgtgcgcggc
    11521 cggatccttc tcgaagatcg tctggtacat gtccagcacc tcgcgattcg cacgatcgcc
    11581 gatcgcatgg atcgccatct ggaacccctc gcgcgccgcg atctccgaca tctgcgtcaa
    11641 atcggccatc ggcgtgacgt tcttgccggt gatgttcggc gcatcgctgt agggctccag
    11701 caaccatgcg ctgcgcgttc ccagcgcgcc atcggaaacg gtttcgccga tgccgcgcac
    11761 ggtgaagtgg ttgtcggcga acccgatggt gcgatgggcg gccaggaact tgtcgaggct
    11821 cgcgaccgac tcaccgtcga cgtacacgta cagccgcagc ggcaggcctt ttgccgcctg
    11881 ctttttcagc cagtcgatgg tttcgaagtc ctcgccctgg tccacgaagg tcgtgatgcc
    11941 cttgctgatc tcgttctgca cggcaagctc gagcgccttc tcgcgccgtg cctcgcgcgc
    12001 cgcaggcggc aacgactgga tgtgatggtt gtaggcggcg aacaccgggt tcgccgcggt
    12061 atcgcgcagc atccctatcg cgcgcccctt cgaatcccgc acgatgctgc cgcccgacgg
    12121 atcaggcgtg tggtcggtga tcccggcgag cttcaatgcc agcgcgttgg catagatgcc
    12181 gtggccgctg gcgtggttga gcaataccgg attgtccggc gacaccgcgt ccagaccgtc
    12241 gggcagcggc aacccgtcga cgttcggctg cgggaccttg ttccacttgg cctgctgcca
    12301 accctgcccg atgatccact ggccgggctt ggccttggcc acggcggctt tcacgattgc
    12361 gacgatctcg tcccaattcg cggccttgcc cacgtcgacc tgcatcaggg cctcgccggt
    12421 tcccatcagg tgcccgtggc cctcgacgaa tcccggcgtc acgaatgcgc cgtgcaggtc
    12481 catcacccgg gtctgcttgc cgatgtagtg cgaaatcgat gcgtcactgc cgacggccag
    12541 gatcttgccg tcgcggaccg ccaacgcctg cgcgttgggc tgtaggggat ccagcgtggc
    12601 gatcgtcccg tcgcgcaata ccaggtcggc gggttgcagt gtaggcggga atcccccgat
    12661 cggggcgctg gaagcgaatg cggtagcgac gacgatgatc gccacgcccg gcagcaacct
    12721 ggaaaaggac ttcatcgaac cctccgttcc tgcgtggggc gcgcatcggc cgcgcacccg
    12781 cgcgccgcga tcctgccccg agaataagtg tcgacggccg ccgtccacca atgaaaagac
    12841 ccgcacctgg cataacctcg cgttgtgacc gacgcgccca tcaggaggcg ccgcctgccg
    12901 cgtccgggcg ttgcgcgaga agcctctggg cctgttgcct gaccaggtcc aggaaacgct
    12961 tctgcaccgg atccagcgga ctgtcgggcc ggtgcatcgc gtacaggatc acgtcgaggt
    13021 gcggtttcag cggacgcatc tggacgctgc ccgcgctgcc gcagcgcgcg gtgaacgggt
    13081 cgaccagcgc caggccctcg cctttcgcga ccaatgcgtg cgccagctga taagtctgca
    13141 ccgagacggt ggtccgcggc gccggatcga cctgcgccag gtggctttgc agcatgctgc
    13201 ccaaggcatc gcgcacggtg atgcctatca tgggctggtc ggccagtgcg ccgatcggca
    13261 acggctgtgc cagttccgaa tcgctccacc accccggcgg ggcgattacc atcacctgtc
    13321 cgcgacacaa aaccttctgg cgcaggttct gctgacccgt atcctgcaag gtcaagccga
    13381 tgtccgtctc gcgcaacgtc agcgattcac acatcgccgc cgaatgctgg gtggacagtt
    13441 cggcgatcgc atcgggaaag gtcttgcgca acaatacggt tgccgcggga agcagcgtca
    13501 gggccagggt gggcgtgcag gtcacccgca gcggacagct tcccggctgg ctcatgttgg
    13561 tcgtgaggcg ctgcagctcg tggacgtcct ggatgatctt ttcgatgcgg tcgcgcagga
    13621 ggaacgcctg ctgcgtcggc tgcaggcgcc cgcgcacgcg gctgaacagg gcgaagccga
    13681 gcttgtcttc tgcgtgctgc aatgccttgc tggccgccgg ctgtgaaatg ttcagcaatt
    13741 tcgcggcgcc ggtcaggctg ccggtggtca ggaccgcatg aaagagttcg atgtatcgca
    13801 gccgcatgtc gagtcgcctt cgagggggtg aacatgtctt tccggatcgt gattgccgcg
    13861 gccctgttgc tcggctccct gcccgccggc gcccggcatg tcgacctgcg cgcctatggc
    13921 atcgcgatcg gcgtcctgcc gaccgggaaa tggaatgccg tcacggacgt gcccggcgtc
    13981 cgggtgggcc agaaaacgct ggtcgcgggg gacgacgtgc gctccggcgt cacggtgatc
    14041 ctgccgcatc cgggcaacgt tttccaaagc aaggttcccg ccgccgtgta cgtcggcaat
    14101 ggtttcggca aactgaccgg aaccacccag gttgccgaac tcggcaacat cgaaacaccc
    14161 atcgtcctga ccaacaccct cagcgtgccc accgccgcgg atgcgctgat cgactatacc
    14221 ttttccttcc ccgccaacgc cgaggtgcgg tcggtcaacc cggtggtcgg cgagaccaac
    14281 gacggcttcc tgaacgatat ccgcggccgc cacgtcacca ggcaggacgt cctcgacgcg
    14341 atccatgccg cgaccggcgg caaggtagca caaggcaatg tcggtgccgg caccggaacg
    14401 gtggcgttcg gcttcaaggg cgggatcggg acttcgtccc ggcaactgcc cgcctcgctc
    14461 ggcggctaca ccgttggcgt gctggtgcag accaatttcg gcggcgtatt gaacatcaat
    14521 ggagtccccg tcggaaagct cctgcaccac tattacctgg gcgacaagct ggatcgggcg
    14581 cccgacccgt cgcccgatgg ctcgtgcatg atcgtggtcg ccaccgacgc gccgctcggc
    14641 tcgcgcaacc tcgggcgcct cgcgaagcgg gcgattctgg gactggcaaa gaccgggggc
    14701 atcgagtcga acgggagcgg cgactatgtg gtcgcgtttt ccaccagcga ggcagtgcgg
    14761 gtgccatacg aatccaaaac cccgctgcaa cccatgacgg tcgtccgcaa cgacgacatg
    14821 tcgccgctgt tcatgggcgc catcgaggcc accgaggagg caatcctcaa ttcgctgttc
    14881 cacgccgaga caatgactgg ccgcgaccac cacacggtcg cggcgctgcc cgtggacaag
    14941 gtcctgcgca tcatgcagca gcgtggctat cccgtcgtga tggctcccgg tgacgcgccg
    15001 gctgaagcca ccagcgcccg gtaagccggc gcggctcgtg ctgcgggcga ggcccctcac
    15061 aactcctggt tatgccacgt acgcgtcttt tcattggtgg tgcccgccgg ccggcaggta
    15121 atctcctgca ggcacgtcgg tggggcgaga catccgaagg aaggactgca tgcgtggcga
    15181 agcgaccggc cggtggggtc ccccttacct tttgtacttg ggcacggcgg ccgacgacct
    15241 ggccatcaag acggcccggg ggctggcgta ctggcgtccc gactggtgcg tgggccagca
    15301 ccggaacccc gcctgcaacc tgaccctcgg attgcccgac ctgggtttcg ccgacgcgag
    15361 gagggcgggc gccgacacca tggtgatcgg caccgccaac gccggcggcg tgctggcgcc
    15421 ggaaaccgtg gccgacatca tcgccgcgct ggatgccggc ctcaacgtga tctcgggact
    15481 ccaccagaag ctgcgcgaca accccgacat cgtggccgcg gccacgcgca atcgccgggt
    15541 gcttttcgac gcacgcgaat tcaaccgcaa cattcccgtc ggcaatggcc gcaagcgcgc
    15601 cggacggcgc ttgctgacgg tgggtaccga ctgctcggtc ggcaagatgt acacgacgct
    15661 cgcgatcgag cgcagcatgc acaaacgcgg cctggtcgcg gattttcgcg ccactggcca
    15721 gaccggcatc ttcgtcgcgg gcggcggcat cccggtggat tcggtgatcg ccgacttcat
    15781 ctccggcgcg gccgaatggc tctcgcccgc gcgcgaggac ggcggctggg acctgatcga
    15841 ggggcaaggc tcgttgttcc atccgtccta cgccggcgtg tcgctgggcc tgctgcacgg
    15901 atcgcagccg gacgcgctgg tgctgtgcca cgaacccacc cggaagcaca tgcgcggact
    15961 gccggactcc ccgatccccg atctcgagcg ttgcctcgag gcgaacgtcg tcgccgcgcg
    16021 gttgaccaac cccgccgcca agccggtcgg tgtcgcgctc aacacttccc agctctccgg
    16081 cgagcaagcg caggcggcct gccgcgaaat cagcgaccgg ctgggcctgc cctgcgagga
    16141 tcccgtcacg atgggcgtcg aggcgatcgt cgacaatctg ctggcatgct gcgcgaactg
    16201 aacatccggc accgcagcct gccgctgcac gcgccgttcc gcatctcgcg cggcgccaag
    16261 catgccgccg acgtggttac cgtcgaactg aagcaagatg gcagcaccgg ccgcggcgaa
    16321 tcggtcccct atgc
//
LOCUS       DASSNH010000005        11238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1001072, whole genome shotgun sequence.
ACCESSION   DASSNH010000005 DASSNH010000000
VERSION     DASSNH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001072"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..589)
                     /gene="def"
                     /locus_tag="VFK00_00180"
     CDS             complement(53..589)
                     /gene="def"
                     /locus_tag="VFK00_00180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7591405.1"
                     /translation="MIREILKMGDPRLLRVAPPVPETMFGSDGLNDLVNDMFETMHAA
                     DGVGLAAPQIGIDLQLVVFGFEHSDRYPGEPEVPRTILLNPLITPLSSDMEEGWEGCL
                     SVPGLRGVVSRHSLIRYRGLDPQGGVIDRTAEGFHARVVQHECDHLIGRLYPSRITDF
                     SKFGFIDVLFPEEVGALE"
     gene            complement(696..1574)
                     /locus_tag="VFK00_00185"
     CDS             complement(696..1574)
                     /locus_tag="VFK00_00185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7591406.1"
                     /translation="MPGSTSILIVSADSGPLLRECVVRALASDAAAEVIVVDNGSRDG
                     IPQAVARAFENEKRLRVLYNHGNLGFGPAVNGAARSAQGDELLVLNPDCLLESDTLAR
                     MRAVLRDHPDAGVVGAVVCDESGKPDPASRRRDPLLRRSLNAMTGRARREGEDSRFEG
                     VDVPGPIPEEVVDVDNVSGALMLLPRAVFDRLQGFDPGYFLHCEDLDLCRRAREAGYK
                     VFLAGGVRVRHGKGGSSAHRPVFVSWHKHRGMWRWFRKFDPAASHPLMRTVVWCGVWL
                     HFAVTAPALWLRRSAA"
     gene            complement(1695..4343)
                     /gene="gspD"
                     /locus_tag="VFK00_00190"
     CDS             complement(1695..4343)
                     /gene="gspD"
                     /locus_tag="VFK00_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HET7591407.1"
                     /translation="MYKKFATLGIVCAAALLAGCVPQVRDHDYSLQREATAGSHQPVP
                     APLDVGTAQQNEPLAMGAQPETHYGTGVFVNVEGTGGKLQGGTNQGGTITFNFDNQPV
                     QAAVKAILGDVLHANYAISPRVKGTVTFATSQPVGKDEVLPILQMLLSWTGNALVQRN
                     GGYLVVPVDEAAPGSLVPGLHAVAPGSGYAAQLFPLHYVSAEAMQKLLKPFADPKAFL
                     LVDPVRNLLVMGGSPDQLANYQRIIHTFDVDWLRGMSVAVIPLQHVQAEDLVSQLQTL
                     FAEQSGASAQQGQQGQRGQQGGRGQQISSLVRLLPLTSSNSLVVITPQPSYVDEVRKL
                     VTTIDNGAGNSSGLYVYNVLNVRASALAQHLNQLFGNATGGGESMQGAVAPGFSPLEQ
                     AAPGSLGAQKGFGTFQQNDTFGGAASGAATGGGFSDSDSGIGAGMVEGATSGRRQQQY
                     GGQGAGVAFTTSEGVRIAAVNENNQLLIRATPGQWEKLLPVIQRLDEKPLQVQIETKV
                     LEVTLKGAFQFGVQYYLGGLIGTQPGSPPFTDESFLRHQGAAGFGGVQYQPDNDTLFY
                     SFAGNRLQFAIRAMESSGNAKVLSAPSTVVLNNQETTFKVGEKIPVVQTYYTPGLGTQ
                     AGNYNVGQVQYIDTGVLLDVVPRVSPGGLVYMDVQQIVSKPQGPPDQFGNFTIANRAL
                     STEVAVQSGQTVLLGGLIQQTDTVTDQGIPFLSRIPVLGRLFGRTDRDKTRSELIVLI
                     TPRIIRDPEDARRITDEYQTQFESLKPILPKNPPAAGEVVPANPAPTPAVAVPAAGAT
                     APAVAATGAWTVQVATFGNDYDAERLHAHLQQLGYAAYTTGVSTPDGQRWRVFAGPVA
                     SKDAAEQLRDHLAEALHIKDMIVERR"
     gene            complement(4385..5098)
                     /locus_tag="VFK00_00195"
     CDS             complement(4385..5098)
                     /locus_tag="VFK00_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591408.1"
                     /translation="MNAADRRRLTPVIGILAVVLAALLVVLWMGLGSGVAWHDTTATA
                     AAQPAVASTTPPPTVEPLDQYSVVWERPLFSPSRTPEAATAGDSGASGDLQLTGVIML
                     PGLKMAILHDKRTGKDYRAIAGQPSHGGPVLVELRPRSAVVESGGSRLQLHLVPGPSA
                     ETGNASPESGGSDGDDGMEPDESAGSGASVMVTRHGAKDSAGRQAAGAGPASNEARAR
                     ALRARIEARRRQAQRDGGG"
     gene            complement(5095..5664)
                     /gene="gspM"
                     /locus_tag="VFK00_00200"
     CDS             complement(5095..5664)
                     /gene="gspM"
                     /locus_tag="VFK00_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HET7591409.1"
                     /translation="MRLTPVQSRLAALLLALVALAAAYFLFLHWWFVAPMLDVGDQMH
                     DLQALHAKYANAIDEQPQLEQRVARLERGGATVGAFLAADDPSAAAAGLIQRATDVVA
                     AHANEGSGCNMPSKMPIEQDNGNEAFRRVSVSITLDCGMQPLASVLHDFDRGLPYLFI
                     DNLSVARSAGGRLQAQLTLSGYLRPAASP"
     gene            complement(5684..6811)
                     /locus_tag="VFK00_00205"
     CDS             complement(5684..6811)
                     /locus_tag="VFK00_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET7591410.1"
                     /translation="MAISSAQTIERTKLAWRRSPLPAFFDWWGGELTALLPVRVREWV
                     RRGRDVLWLGADSGRLTVRRARTGTTLASVPRESPMEGQRGEFARVCAGIDPDDRRLL
                     LVVPADQVLVRRLVMPAAAAADPRRVAGYELDRQTPFTAEQVYYDVRLSTEQPAAGQI
                     ALDLYVAPKAVLDPVLEHFKAIGAQPDGVDVQAADGHLAGVDLLPPGQRPRHMEKRRR
                     ANWLLAAVCVLLAVLVLLQWLDNRRAALATMQDEVAAMRAKATRSQQLHAQLTGALAA
                     SKFLVKRKAGNPSALAVLDDLTRRLPATAWLDSLTLDDSGGLDIRGEADKAASLVDTL
                     GNSRILSEPKLQGVIQPDPATGKERFELVARVRGAGAAGAH"
     gene            complement(6821..7792)
                     /locus_tag="VFK00_00210"
     CDS             complement(6821..7792)
                     /locus_tag="VFK00_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HET7591411.1"
                     /translation="MTSGLPRRRAHPLRQDAAAHSRGRERGTALLLVLWATMLLATLL
                     VGVAAASRSHSEAALYGAERVRAELAAEAGIAHAVAGLRSPGAGAQWVPDGRPHVFAF
                     DGAKVTVAVVDTTGQVDLNASPPELLRSLFQAAGADASRAERLAQAVVQFRGGASRGI
                     GILGPVVPRTAAPSHGPFRAIDQLAMLPGMDSELFDGVAKAVTVFSGRNFPDASYAGP
                     LAVAALRGIGPAEANALVEARRRRPAQGGAGNGQALGAVAGGPLVAGYGGVVQRVFST
                     AEMPDGARINLDVTIRMALTGAGARPYKVLDWRNDSEWRTHSEGTVK"
     gene            complement(7789..8553)
                     /locus_tag="VFK00_00215"
     CDS             complement(7789..8553)
                     /locus_tag="VFK00_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7591412.1"
                     /translation="MNHEKRERGMGNGQSRRPALRRRCCLFPVPRFPRAKHANGFTLL
                     EILLALALLALVMLGVWSALAGAVRITHSAGAVMARSESVHMAQQFLRRYVADAGMHP
                     WVAEAGAGTRARVFAGDAMSMQYVAPLPMQSGHAGLYLQTLRIQKQDDGGLALWLSYE
                     PYTANVPASGKPERHELLSDLDGGSFQYLGGGAFGEPARWRDDWADANGLPLAVRIHL
                     EPHWRERVPVPDMLIALHSGEVVPQAGTTGQTGTLQ"
     gene            complement(8550..9077)
                     /locus_tag="VFK00_00220"
     CDS             complement(8550..9077)
                     /locus_tag="VFK00_00220"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7591413.1"
                     /translation="MNGGKREQGTGNPERAGGGVRSSLFPVPHSRRAARAGGFTLLEV
                     LAALAVLAIAFAIGLGALGKSAQNAVRSAALDTAVERAQSVLAEQGLMAPLQDETLRG
                     KFDDGMSWTLRIHALPRPATGNGEAVALQRQGVLLAQAGAIDIYQLDIAVQYGAGRVL
                     RLSTQRAQAAAEPQP"
     gene            complement(9074..9637)
                     /locus_tag="VFK00_00225"
     CDS             complement(9074..9637)
                     /locus_tag="VFK00_00225"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET7591414.1"
                     /translation="MITEIGNRPENREQGRGNREQKRPNLASFSRPRFPVPRFRRAQR
                     ARGFTLIEILAVIVLIAIGVTIAAVSLHGTGRGRLDAAAQQVAAGLRDTRTRAMATSQ
                     PQAFAVDLRAHTFSAPGRDPRKLPVGAAVQVTSAAEDTTGQGVARIRFFPDGSSSGGH
                     VELSEAHRTVRIDVDWLTGAVAVSGKP"
     gene            complement(9615..10166)
                     /locus_tag="VFK00_00230"
     CDS             complement(9615..10166)
                     /locus_tag="VFK00_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET7591415.1"
                     /translation="MSPMILSGTGDRGPGTNSPDIRPSDQAPGSGGAETYPAAGKRAR
                     RRSRVPGPRSLGFTLLEMLAVIVLLGIVAVIVARSVSGRVDTGKWKAGTIGVTKLDQD
                     VQAYALDNGSPPKSLDDLIAKPLNAPSWNGPYAKPADLKDPFGHPYGYVYPGKHGQYD
                     IVFWGRDGKPNGEGVDRDYGNWQ"
     gene            complement(10189..>11238)
                     /locus_tag="VFK00_00235"
     CDS             complement(10189..>11238)
                     /locus_tag="VFK00_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7591416.1"
                     /translation="GLRKKSLAGAALVEFTDQLGVLLHAGLPLDRALQMMLELPEGER
                     ARRVVGRIRDRVRGGSSLSRAMEEEHGVFPKLYLSMVRAGEAGGALDAALARLADYLT
                     RARKLRESVIGALVYPAFLMFGVLGSLVLLLAFVLPQFVPIFHDMGVRIPLITRVLMA
                     IGSFLHDWGLLLLAAIVAAGLWAGARMRDPEVRRGFDARLLRVKVLGPLLLKVDTARL
                     MRTLGSMLKNGVSLLAALGIARQVVSNRDLSAQLAPAIEAVKGGDSLSHALAAHTGFP
                     RLAIQITTVGEESGTLDTMLLRAADTYDGEVKSAIDKMLSALVPILTIVMALLVAGIM
                     LSILLPLLSLTGSIQ"
BASE COUNT         1772 a         4008 c         3739 g         1719 t
ORIGIN      
        1 ttatccgcgt caaaagcgct tttgatccga tcgagaaaag gcgacgaatc gcctactcca
       61 gcgcgccgac ttcctcgggg aacagcacgt cgatgaaacc gaacttcgag aagtcggtaa
      121 tccgcgaggg atagagccgc ccgatcaggt ggtcgcattc gtgctgcacc acccgcgcgt
      181 ggaagccttc ggcggtgcgg tcgatcacgc cgccctgcgg atccagcccg cggtagcgaa
      241 tcaggctgtg gcggctgacg acgccgcgca ggcccgggac cgacaggcag ccctcccagc
      301 cttcctccat gtcgctggac agcggcgtga tcagcgggtt cagcaggatc gtgcgcggca
      361 cttcgggctc gccggggtaa cggtcggaat gctcgaagcc gaacaccacc agttgcaggt
      421 cgatgccgat ctgcggcgcg gcgagcccca cgccatcggc agcatgcatg gtctcgaaca
      481 tgtcgttgac gagatcgttc aacccgtcgc tgccgaacat cgtttccggc accggcggcg
      541 ccacccgcaa caggcgcgga tcgcccatct taaggatttc acggatcatc gctgcagcac
      601 cttgcatgct cctctcgcgt ttgcgacgca gtgtacatag cgagccgtgc acgcgtcgcg
      661 caccgcgacc gcctttccac cggaagaatg ctgaatcagg cggcgcttcg gcgcagccac
      721 agggcagggg cggtgaccgc gaagtgcaac cagacaccgc accatacgac ggtgcgcatc
      781 agcggatggc tggccgcagg atcgaacttg cggaaccagc gccacattcc gcggtgcttg
      841 tgccaactga cgaacacggg ccggtgcgcg ctggacccgc ccttgccatg ccgcacgcgc
      901 acgccgcccg ccaggaacac cttgtagccc gcctcccgcg cacgccggca gaggtcgaga
      961 tcctcgcagt ggaggaaata accgggatcg aatccttgca ggcggtcgaa gaccgcgcgg
     1021 ggcagcagca tcaacgcacc cgacacattg tcgacgtcga caacctcttc cggaatgggc
     1081 cccggcacgt cgacgccttc gaagcgcgaa tcctcgccct cgcgccgcgc acgtcccgtc
     1141 atcgcgttca acgatcggcg caacaacgga tcgcgtcgcc gtgacgcagg atccggtttg
     1201 ccggattcgt cgcagaccac cgcgcccacc acgccggcgt ccggatggtc gcggagcacc
     1261 gcccgcatgc gcgccagcgt gtcgctttcc agcaggcaat ccggattgag caccagcagc
     1321 tcgtcgccct gcgccgagcg cgctgcgccg ttcaccgccg gcccgaaccc gaggttcccg
     1381 tggttgtaga gcacccgcag gcgcttttcg ttctcgaacg cgcgcgccac cgcttgcgga
     1441 atcccgtcgc gcgacccgtt gtccaccacg atcacttccg cagccgcgtc ggaggcgagc
     1501 gcgcgcacca cgcactcgcg caacagcgga ccgctgtcgg cggagactat gaggatggat
     1561 gtggagccgg gcacccgttt agtctagggc cggcgaaccg ctacgaacat cgtgccaccc
     1621 gcgcacgcac ggctccggca gcgatcgcgt ccaaacccgg gagcgtccag actgccgggc
     1681 cttcgccgcg agcgctagcg ccgttcgacg atcatgtcct tgatgtgcaa ggcctcggcg
     1741 aggtggtcgc gcaattgctc cgcggcatcc ttgctggcca ccggccctgc gaacacgcgc
     1801 cagcgttgcc cgtcgggggt cgatacgccg gtcgtgtagg cggcatatcc gagttgctga
     1861 agatgcgcgt gcagtcgctc ggcgtcgtag tcgttgccga acgtggcaac ctggacggtc
     1921 caagcacccg ttgccgccac ggccggcgca gtcgcgcctg ccgcgggaac cgcgacggcg
     1981 ggcgtcgggg ccggattggc gggcaccact tcgcctgcgg cgggcgggtt tttcggcagg
     2041 atcggcttca gcgattcgaa ctgggtctgg tattcgtcgg tgatgcgccg tgcatcctcg
     2101 ggatcccgga tgatccgcgg cgtgatcagc acgatcagct cgctgcgggt cttgtcgcga
     2161 tcggtcctgc cgaacaggcg ccccagcacc gggatgcggc ttaggaaagg gatgccctgg
     2221 tcggtgaccg tatcggtctg ctggatcagg ccgccgagca agacggtctg gccgctctgc
     2281 accgcgactt cggtactcaa ggcgcggttg gcgatggtga agttgccgaa ctggtccggc
     2341 ggaccctgcg gcttgctgac gatctgctgc acgtccatgt acaccagtcc gccggggctg
     2401 acgcgcggca ccacgtcgag cagcacgccg gtgtcgatgt actgcacctg gcccacgttg
     2461 tagttgcccg cctgcgttcc gaggcccggc gtgtagtagg tctgcaccac cgggatcttt
     2521 tcgcccacct tgaaggtggt ttcctggttg ttgagcacaa ccgtggaggg cgccgacagc
     2581 accttggcgt tgccgctcga ctccatcgcg cggatcgcga actgcaatcg attgcccgcg
     2641 aacgaataga acaacgtgtc gttgtccggc tggtactgga cgccgccgaa gcccgcggcg
     2701 ccctggtggc ggaggaagga ctcgtcggtg aacggcggcg atcccggctg ggtgccgatc
     2761 agcccgccga ggtaatactg cacgccgaac tggaaggcgc ccttcagggt cacttccagt
     2821 accttggtct cgatctgcac ctgcagcggc ttctcgtcca gccgctggat caccggcaga
     2881 agtttctccc actggcccgg cgtcgcgcgg atcaacaact ggttgttctc gttgaccgcg
     2941 gcgatgcgca cgccttcgct ggtggtgaag gcgactcccg cgccctggcc gccatattgc
     3001 tgctggcggc gcccgctggt cgcgccctcg accatgcctg cgccaatgcc gctgtcactg
     3061 tcactgaacc cgccgcccgt ggcggcgccg gaagcggcgc ctccgaacgt gtcgttctgc
     3121 tggaacgtgc cgaaaccctt ttgtgcgcca aggctgccgg gcgcggcctg ctcgagcggc
     3181 gaaaagccgg gcgccacggc gccctgcatc gattccccgc cgcccgtggc gttgccgaac
     3241 agctggttga ggtgctgcgc cagcgccgac gcacggacgt tcagcacgtt gtacacgtag
     3301 agccccgagg aattgccggc gccgttgtcg atcgtggtga ccagcttgcg cacctcgtcg
     3361 acgtaggagg gctgcggcgt gatcacaacg aggctgttgc tgctggtgag cggaagcagc
     3421 cggaccaggc tggatatttg ctgtccccgc ccgccttgct gaccccgctg gccttgctga
     3481 ccctgctggg cgcttgcgcc ggactgttcg gcgaacaagg tctggagttg gctgaccagg
     3541 tcttccgcct gcacgtgctg gagcgggatc accgccaccg acatgccgcg cagccagtcg
     3601 acgtcgaagg tgtggatgat gcgctggtag ttggcgagtt gatccgggct accgcccatc
     3661 accagcaggt tgcgtaccgg gtcgaccagc aggaaggcct tgggatcggc aaacggcttc
     3721 aagagcttct gcatcgcttc cgccgacacg tagtgcagcg ggaacaactg cgccgcatag
     3781 cccgacccgg gcgcgaccgc gtggagccca ggcaccagcg acccgggcgc cgcctcgtcg
     3841 accggcacca ccaggtaccc gccattccgt tgcaccaggg cgttgccggt ccacgacagc
     3901 agcatctgca ggatcggcaa cacctcgtcc ttgccgaccg gctgcgaggt cgcgaaggtc
     3961 acggtgccct tgacccttgg cgagatcgcg taattggcgt gcaacacgtc gccgagaatc
     4021 gccttgaccg ccgcctgcac cggctggttg tcgaagttga aggtgatcgt gccgccctgg
     4081 ttggtgccgc cttgcagctt cccgccggtg ccttccacgt tgacgaacac accggtcccg
     4141 tagtgggttt ccggctgggc gcccatcgcc agcggctcgt tctgctgcgc cgtgcccacg
     4201 tcaagcggcg ccggcaccgg ctggtggctg ccggcggtgg cttcgcgttg gaggctgtag
     4261 tcgtggtcgc ggacctgcgg cacgcaacct gccagcaacg cggcggcgca cacgattccc
     4321 agcgtggcaa acttcttgta cacgtgatga tctccgccgt tgaccctagg tcggatcgcc
     4381 gccctcagcc gccgccgtcc cgttgcgcct ggcgcctgcg cgcctcgatg cgcgccctga
     4441 gcgcgcgggc acgcgcctcg ttcgatgccg gtccggcgcc cgccgcctgc ctccctgccg
     4501 aatccttcgc tccgtggcgc gtgaccataa cagaagcgcc gctgccggcg ctctcatccg
     4561 gctccatgcc gtcatccccg tccgacccgc cggattccgg cgaggcgttg cccgtctccg
     4621 ccgacgggcc gggaaccagg tggagttgca ggcgcgagcc accggattcc accacggcgc
     4681 tgcgcggacg caactccacc agcaccggcc cgccgtggga cggctggccg gcgatggcgc
     4741 ggtaatcctt gccggtgcgc ttgtcgtgca ggatcgccat cttcagcccg ggcagcatga
     4801 tcacgccggt caattgcagg tcgccggacg cccccgaatc gccggccgtc gccgcttccg
     4861 gcgtccggct cgggctgaac agcgggcgct cccagaccac gctgtactgg tcgagcggtt
     4921 ccaccgtcgg gggtggcgtg gtcgacgcga cggcgggctg ggccgccgcg gtcgcagtcg
     4981 tgtcgtgcca ggcgacgcca ctgcccagcc ccatccagag cacgaccagc agcgccgcca
     5041 gcaccacggc gaggatgccg atcaccggcg tcaggcggcg gcggtcggcg gcgttcatgg
     5101 cgaagccgcc gggcggaggt agccggacaa ggtcaattgc gcctgcagcc gcccgccggc
     5161 ggaacgcgcg acgctgaggt tgtcgatgaa gagataggga aggccgcggt cgaagtcgtg
     5221 cagcaccgac gccagcggct gcatgccgca atcgagcgtg atgctgacgc tgacgcgccg
     5281 gaacgcctcg ttgccgttgt cctgctcgat cggcatcttg gacggcatgt tgcagcctga
     5341 gccttcgttg gcgtgggccg cgaccacgtc ggtagcgcgc tggatcaatc cggccgcggc
     5401 agcgctcgga tcgtccgcgg cgaggaacgc accgaccgtc gcgccaccgc gctccagtcg
     5461 cgcgacgcgc tgctccagtt gcggctgttc gtcgatcgcg ttcgcgtact tcgcgtgcag
     5521 cgcctgcaga tcgtgcatct ggtcgccgac atccagcatc ggggcgacga accaccagtg
     5581 caggaacagg aaatacgccg ccgccagcgc gaccagcgcc agcagcaggg ccgcgagacg
     5641 cgactgcacc ggcgtcagcc gcacggcgcc ccctcgccga cggtcaatgc gcaccggcag
     5701 cgcccgcgcc gcgcacgcgc gcgaccagtt cgaagcgttc cttgccggtg gcagggtcgg
     5761 gctggatcac gccctgcagc ttcggctcgc tcaggatcct ggagttgccg agggtatcga
     5821 ccagcgacgc ggccttgtcg gcttcgccgc gaatgtcgag cccgccggaa tcatccagcg
     5881 tcaggctgtc gagccacgcc gtggccggca ggcgtcgggt caggtcatcg agcacggcga
     5941 gcgcggacgg attccccgcc ttgcgcttga ccaggaactt cgaggccgcg agcgccccag
     6001 tcaattgtgc gtgcaactgt tgcgaacgcg tggccttagc gcgcatggcg gcgacctcgt
     6061 cctgcatcgt cgcgagcgcg gcacgccggt tgtccagcca ctgcaacagc accagcacgg
     6121 ccagcagcac gcacaccgcg gcgagcaacc agttggcgcg ccggcgcttc tccatgtgcc
     6181 gcggacgctg acccggcggc aacaggtcca cgccggccag gtggccgtcc gcagcctgca
     6241 cgtccacgcc gtcgggctgc gcaccgatcg ccttgaaatg ttccagcacc ggatcgagta
     6301 ccgccttcgg cgcgacatag agatcgaggg cgatctgccc ggccgccggc tgctcggtgc
     6361 tgaggcgcac gtcgtaatac acctgctcgg cagtgaaggg cgtctgccgg tcgagttcgt
     6421 agccggccac gcgccgcggg tcggccgccg ccgccgcggg catcaccagg cgccgcacca
     6481 gcacctggtc cgcaggcacc accagcaaca gccggcggtc atccggatcg atgccggcgc
     6541 agacccgcgc gaattccccg cgctgcccct ccattggcga ttcccgaggc acgctcgcga
     6601 gggtcgtgcc ggtacgcgcg cggcgcaccg tcaagcggcc ggaatccgca ccgagccaga
     6661 gcacgtcgcg gccccgccgc acccactcgc gcacgcgtac gggcaacagc gcggtcagct
     6721 cgccgcccca ccaatcgaag aacgccggca gcggcgagcg ccgccacgca agcttcgtgc
     6781 gctcaatcgt ttgtgcgctt gatatcgcca tggcggtgga ttatttcacg gtgccttcgg
     6841 aatgggtgcg ccactcggaa tcattgcgcc aatcgaggac tttgtacggg cgggcgccgg
     6901 ctcccgtcaa cgccatgcga atcgtgacgt cgaggttgat ccgtgcaccg tcgggcattt
     6961 ccgccgtgct gaatacccgc tggaccacgc cgccgtagcc cgcgaccagc ggaccaccgg
     7021 caaccgcgcc cagcgcctgg ccgttgccgg cgccgccttg cgcgggccgg cgccgccgcg
     7081 cctccaccag cgcgttcgcc tccgcgggac cgatgccgcg caatgcggcg acggccaggg
     7141 gcccggcgta cgacgcatcc ggaaagttgc gaccggaaaa aaccgtcacc gccttcgcga
     7201 ccccgtcgaa cagctccgag tccatgccgg gcagcatggc caactggtcg atcgcgcgaa
     7261 acgggccgtg cgatggggcg gcggtgcgcg gcaccacggg gccaaggatg ccgatgccgc
     7321 gcgacgcgcc gccgcgaaac tgcaccaccg cctgcgcaag ccgctcggcg cgcgatgcat
     7381 ccgcgcccgc cgcctggaac aggctgcgca acaactcggg ggggctcgca ttgaggtcca
     7441 cttgtccggt cgtgtcgacg accgcaaccg tcaccttcgc gccgtcgaac gcaaagacat
     7501 gcgggcgtcc gtccggcacc cattgcgcgc cggcaccggg cgagcgcaag ccggcgaccg
     7561 cgtgcgcgat gccggcttcg gcagcaagct cggcgcgcac ccgctccgca ccgtacaacg
     7621 ccgcctcgct gtggctgcgg gacgcggcgg ccacgccaac caacagcgtc gccagcaaca
     7681 ttgttgccca caacaccagc agcagggcgg tcccgcgttc gcggccacgg gagtgcgccg
     7741 ctgcgtcctg ccggagcggg tgggcacgac gtcgagggag gccactggtc attgcaatgt
     7801 cccggtctgc cctgttgtcc cggcctgcgg cacaacttcg ccgctgtgta gggcgatcag
     7861 catgtccggg accggcaccc gctcacgcca atgcggctcc agatggatcc ggacggcgag
     7921 cggcaagcca ttcgcgtccg cccagtcgtc ccgccaccgg gccggctcgc cgaaagcgcc
     7981 cccgccaagg tactggaagc tgccgccgtc gaggtcggag agcaactcgt gccgctcggg
     8041 cttgcccgac gccggcacgt tggccgtgta aggctcatag ctgagccaga gcgcgaggcc
     8101 accgtcatcc tgtttctgga tccggagcgt ttgcaggtag aggccggcat ggcccgattg
     8161 catgggtagc ggcgcgacgt actgcatcga catggcgtcg cccgcgaaca cccgggcacg
     8221 cgtgccggcg cccgcctccg ccacccacgg gtgcatcccg gcgtcggcga cgtagcgacg
     8281 caggaactgc tgcgccatgt gcacgctctc gcttcgcgcc atcaccgcac cggccgaatg
     8341 ggtgatccgc accgcgcctg cgagcgcgct ccagacgccg agcatcacca acgccagcag
     8401 cgccagtgcc agcaggatct ccagcagggt gaagccgttg gcgtgcttcg cacgcgggaa
     8461 acggggaaca gggaacaggc aacaacggcg tcgcagcgcg gggcgccgtg attgcccatt
     8521 ccccattccc cgttcccgct tttcgtgatt cacggctgcg gctccgccgc agcctgcgcg
     8581 cgctgggtcg acagccgcag aacccggccg gcaccgtatt gcaccgcgat gtcgagctgg
     8641 tagatgtcga tggcgccggc ttgcgcgagc aacacgcctt gccgctgcag cgccacggct
     8701 tcgccgttgc ccgtcgccgg ccgcggcaac gcgtggatcc ggagcgtcca gctcatcccg
     8761 tcgtcgaact tgcctcgcag cgtctcatcc tggagcggcg ccatcagtcc ctgttccgcc
     8821 agcacgctct gcgcgcgctc gaccgccgtg tcgagcgccg cactgcggac ggcgttctgc
     8881 gccgacttgc cgagcgcgcc gaggccgatc gcgaacgcaa tcgccagcac cgcgagcgcg
     8941 gcgaggactt cgaggagggt gaagccgcca gcgcgcgcgg cgcgccggga atggggaacg
     9001 gggaacaggg acgaacgcac gccgccgccc gcacgctccg ggttccctgt tccctgttcc
     9061 ctcttcccgc cgttcatggc ttgccactca ctgccaccgc tccggtaagc caatccacgt
     9121 cgatccgcac cgtccgatgc gcctcgctga gctcgacgtg gccgccgctc gagctgccgt
     9181 ccgggaagaa ccggatgcgc gccacgcctt ggccggtggt atcttcggcc gccgacgtga
     9241 cctgcaccgc cgcgcccacg gggagcttgc gcggatcgcg tccgggcgct gagaacgtgt
     9301 gggcgcgcag gtccacggcg aatgcctgcg gctggctggt tgccatcgcg cgggtgcggg
     9361 tatctcgcag ccccgccgcc acctgctgcg ccgcggcgtc caggcggccg cggccggttc
     9421 cgtgcaggct gaccgcggcg atggtgaccc cgatcgcgat cagcacgatc accgcgagga
     9481 tttcgatcag ggtgaaaccg cgggcgcgct gcgcgcgccg gaaacgggga acggggaacc
     9541 ggggacggga gaagcttgcg agattcggcc gcttttgctc cctgttcccc cttccctgtt
     9601 cccgattctc aggcctattg ccaatttccg taatcacgat cgacgccctc gccgttcggc
     9661 ttgccgtcgc gcccccagaa gacgatgtcg tactgcccgt gcttgccggg atacacgtat
     9721 ccgtatggat ggccaaacgg atccttcagg tcggccggct ttgcgtacgg cccgttccag
     9781 gatggcgcat tgagcggctt ggcgatcagg tcgtcgagcg acttcggcgg cgaaccgttg
     9841 tcgagcgcgt atgcctgcac gtcctggtcg agcttggtga cgccgatggt gccggccttc
     9901 cacttgccgg tatcgacccg acccgaaacc gagcgcgcga cgatcaccgc gacgatgccg
     9961 agcagcacga tcacggcgag catttcgaga agggtgaagc cgagggaccg gggaccgggg
    10021 acccgggacc ggcggcgggc gcgctttccc gcagcagggt aagtctccgc accgcccgat
    10081 cccggtgcct gatcggaagg ccggatgtcg gggctgttgg tccccggtcc ccggtccccg
    10141 gtcccggaca aaatcattgg cgacatgacg aaaccctttc gttgatagtc attggatgct
    10201 gccggtgagg ctcaacagcg gcagcaggat ggaaagcatg atgccggcga ccagcagcgc
    10261 catcacgatg gtgaggatgg gcaccagcgc cgacagcatc ttgtcgatcg cggacttgac
    10321 ctcgccatcg taggtgtcgg cggcccgcag caacatggta tcgagggtgc ccgactcctc
    10381 gccgaccgtg gtgatctgga tcgcgagcct cgggaagccg gtgtgggcgg cgagcgcgtg
    10441 cgacagcgaa tcgccgccct tgaccgcctc gatcgcgggc gcgagttgcg ccgacaggtc
    10501 gcgattggac accacctgtc gcgcgatgcc gagcgcggcg agcaacgaca cgccattctt
    10561 cagcatcgag ccgagggtgc gcatcaggcg cgcggtgtcg accttcagca gcagcggacc
    10621 gagcaccttg acgcgcagca ggcgggcatc gaagccgcgt cggacctcgg gatcgcgcat
    10681 ccgcgcgccg gcccacaagc ctgcggccac gatcgccgcg agcaacagca agccccagtc
    10741 gtgcaggaac gatccgatcg ccatcagcac gcgcgtgatc agcgggatcc gcacgcccat
    10801 gtcgtggaag atcggcacga actgcggcag cacgaacgcc agcaacagca ccagcgaacc
    10861 gagcacgccg aacatcagga acgccgggta caccagcgcc ccgatcacgc tctcgcgcaa
    10921 cttgcgcgcg cgcgtcaggt aatcggcaag gcgcgccaag gccgcgtcca gggctccgcc
    10981 ggcctcgccc gcacgcacca tgctcaggta cagcttcggg aacacgccgt gttcctcttc
    11041 catcgcccgc gacagcgaag agccgccacg gacgcggtcg cggatccggc cgaccacgcg
    11101 gcgcgcccgc tcgccttccg gcagctccag catcatctgc aacgcgcggt ccagcggcag
    11161 ccccgcgtgc agcagcacgc cgagctggtc ggtgaactcg accagcgcgg caccggccag
    11221 cgacttcttg cggaggcc
//
LOCUS       DASSNH010000006         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1883292, whole genome shotgun sequence.
ACCESSION   DASSNH010000006 DASSNH010000000
VERSION     DASSNH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883292"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFK00_00240"
     CDS             <1..201
                     /locus_tag="VFK00_00240"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398410.1"
                     /note="NH(3)-dependent; catalyzes the formation of
                     nicotinamide adenine dinucleotide (NAD) from nicotinic
                     acid adenine dinucleotide (NAAD) using either ammonia or
                     glutamine as the amide donor and ATP; ammonia-utilizing
                     enzymes include the ones from Bacillus and Escherichia
                     coli while glutamine-utilizing enzymes include the
                     Mycobacterial one; forms homodimers; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7591417.1"
                     /translation="ILQRCIEGAQSREEIVAAGYNAADVQRVVRMLYAAEFKRRQSAP
                     GPRVSPCAFGRERRYPISNGWS"
     gene            complement(469..1305)
                     /locus_tag="VFK00_00245"
     CDS             complement(469..1305)
                     /locus_tag="VFK00_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET7591418.1"
                     /translation="MPFRRPAPLLKFALLLPLLLTLGACFHHGERDTLNTMTVEQLYE
                     RGHSLSEGGNWGAAETVYQKLISRFPYGAYNEQAQIELAYAQYKEDKPDDAYSTINEF
                     IKTYPTQKHVAYAYYLRGLINFDRTESALQRMVGISPSRFDQGYALQSFDDFNTLVAR
                     FPDTRYAADARQRMVYLRNQLAQSELNVAEFYLQRQAYIAAADRAKYVIEHYQRSPET
                     ADALAILAKSYHQLGQQKLADDTTQVLKLNYPDHPYLRDPDGWPDHHTWVYRLIPLTS
                     AH"
     gene            1377..2348
                     /gene="rluD"
                     /locus_tag="VFK00_00250"
     CDS             1377..2348
                     /gene="rluD"
                     /locus_tag="VFK00_00250"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HET7591419.1"
                     /translation="MTERRLRAEIPTDVAGPRFDQILPRLFPGYSRAHLAAEVRAGRI
                     RLDGRQVPARTRVHGGEEVEWQDVEQPVVGDRPEAIELAIVFEDADLLVIDKPAGLVV
                     HPGAGNPAGTLLNALLHHDPGLARIPRAGIVHRLDKDTSGLLVVARSLRAHTALTRML
                     AERDIHRRYAAIVNGVPVAGGTVDAPLDRHPSDRLRRAVREGGRPAVTHYRVREKFRA
                     HALLQCELESGRTHQIRVHMAHAGYPLVGDALYGKGVRLPRGASPALADALRGFRRQA
                     LHAERLAFAHPVTEEAISFEAEPPQDLLDLAAALREDAHAPAPRPGS"
     gene            2407..3174
                     /gene="pgeF"
                     /locus_tag="VFK00_00255"
     CDS             2407..3174
                     /gene="pgeF"
                     /locus_tag="VFK00_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET7591420.1"
                     /translation="MRPMWILPDWPRPPNVRAVTTTREGPSPSRFGFNVGTRCGDEPG
                     AVARNRTFIREVLRLPSEPCWLRQVHGTRVVTLDTPPQRDESEGDPIVEREPEADAAV
                     TRSTGVVLAIQTADCLPVLLGAEDGCEIAAAHAGWRGLSAGVLENTLAAMRTPPASVI
                     AWLGPAIAAASYEVGEEVRDAFLSHGAAATSAFSATRPGHWTCDLYELARQRLRAAGV
                     TCIAGGGFDTFKDPRLHSYRRDGAGSGRMASLVWIGG"
     gene            complement(3242..4606)
                     /gene="otsA"
                     /locus_tag="VFK00_00260"
     CDS             complement(3242..4606)
                     /gene="otsA"
                     /locus_tag="VFK00_00260"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HET7591421.1"
                     /translation="MKRLVVISNRVAQPRETRAGGLAAAMRSALKEHGGLWFGWSGKL
                     VAESERTLHRESAGSIDYALLDLTREEYDAYYLGFSNRTLWPLLHFQPTMMQYSRGQY
                     AGYRAVNKLFAKKIAPLLRDDDHIWIHDYHLIPLAAELRALGVGARMGFFLHIPLPPP
                     ELLVNLPCHLDLMATLAAYDLVGVQVKDDASALFRYFLGVLGTSRSGDVIELDGRHTR
                     IAPFPISIDTAEVAHQAQSVLGNSAIRKLRASLEDRKLAIGVDRLDYSKGLPRRFSAF
                     GKFLAEHREWRSRVSFLQIAPPSRQEVPEYRELRSRLERSAGATNGRYAEPDWVPIRY
                     VNRSFAQTTLAGFYRISQLALITPLRDGMNLVAKEFVAAQNPDDPGVLVLSRFAGAAN
                     ELPQAILVNPYDPERLVEGIEEGLTMSRAERRERWAGMFEHLSTHDINAWRRDFIGAL
                     EEHG"
     gene            complement(4603..6366)
                     /locus_tag="VFK00_00265"
     CDS             complement(4603..6366)
                     /locus_tag="VFK00_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7591422.1"
                     /translation="MDLGIIGNGTVNALIDARGRVVWHCVPAFDGDPVFCSLLSPKLG
                     DTGFFDVELENETRCQQRYVDNSAVLVTVLTAEDGSQIEITDFAPRYKLHERTYHPMQ
                     ILRRVRPLSGSPRIAIRLRPLTDYGGRKPVHTFGSNHVRFMLDPLVLRVTSDAQLPML
                     RDELPFLLDRPVHMVLGTDETLQESAGHYFDEALNATLAYWHEWSRYLSIPAEWQEAV
                     IRAAITLKLCQYEGTGAIVAAMTTSIPEAPDTVRTWDYRYCWPRDAALSVRALNRLSA
                     TRTMEEYVRYIFNLAVKNDTMTPVYGIHFQDELPEREAATLAGYRGMGPVRVGNEAWR
                     QQQNDIYGSVVLAATQMFFDQRLVKIGDETEFRRLERCGEMAWKLHDKPDAGLWELRG
                     RQSVHTYSAVMCWVACDRLCNIATWLQLPDRAKFWRKRADRIHELVLKRAVHKDGYFV
                     ESFGSEDLDASLLLLEGLRFVSADDPRFVKTVEAVGETLMRDGFLLRYAQPDDFGAPK
                     TSFMVCNFWYVEALIAIGRKNEARQLFEKLLAMRNPLGLLSEDLDPRRRELWGNFPQT
                     YSLVGLINAARGLSTKWSDVV"
BASE COUNT         1017 a         2071 c         2240 g         1058 t
ORIGIN      
        1 atcctgcaac gctgcatcga gggcgcccag tcccgggaag agatcgtggc ggccggttac
       61 aacgccgccg acgtgcaacg ggtggtgcgg atgctgtatg cggcggagtt caagcgccgc
      121 cagtcagcgc cgggtccgcg cgtgtcgccg tgcgcgttcg gccgcgagcg gcggtatccc
      181 ataagcaacg gctggagttg atgcggttgc cggcttccgt tcgggaggat gcgatggcgt
      241 cgcccgcaca gcgcttgtcg gagcggccca tggccgcggc ccacatcttg aggcaacccg
      301 accagcgatt gcggcaacgc ctggcgcgaa tggcgcgctg ctacaacagc cgggtagggc
      361 gcgttggccg cgcgagatgc cggcgggcgg tgtccgccct atgcgcaccc gatgacgccc
      421 cgggtggcga aggaattcgg cagtgccgca gccgcggtac gcgtcgtgtc agtgcgcgct
      481 ggtcagcggg atcagccggt agacccaggt gtggtggtcg ggccagccgt ccggatcacg
      541 caggtacggg tggtcgggat agttgagctt caggacctgg gtggtgtcgt ccgcgagctt
      601 ctgctggccg agctggtggt agctcttggc caggatcgcc agagcgtctg cagtttccgg
      661 cgagcgctgg tagtgttcga tcacgtactt ggcgcggtcg gccgcggcga tgtaggcctg
      721 ccgctgcagg tagaactcgg cgacgttgag ttccgactgc gcgagctggt tgcgcaggta
      781 gaccatgcgc tggcgcgcgt cggcggcgta gcgggtgtcg gggaagcgcg cgaccagcgt
      841 gttgaaatca tcgaacgact gcaacgcgta gccctggtcg aagcgcgacg gcgagatccc
      901 gaccatccgc tgcagcgcgg attccgtgcg gtcgaaattg atcaggccgc gcaggtagta
      961 cgcgtaggca acatgcttct gggtcggata ggtcttgatg aattcgttga tggtcgaata
     1021 cgcatcgtcg ggcttgtctt ccttgtactg cgcgtaggcg agctcgatct gcgcctgttc
     1081 gttgtaggcg ccgtacggaa agcgcgagat cagcttctgg tagacggtct cggccgcgcc
     1141 ccaattgccc ccttccgaca gcgaatgccc gcgctcgtac aactgctcga cggtcatggt
     1201 gttcagggtg tcgcgctcgc cgtggtggaa gcaggcgccc aaagtgagca ggagcggcag
     1261 caacagcgcg aatttcagca gcggtgcggg acgtcggaat ggcatcgggg gagtctttgc
     1321 gaacgcgaac gggcatgata gcggaatatg tatcacccaa cccggagaca ggctgcgtga
     1381 ctgaacgacg actccgcgcg gaaatcccga ccgacgtggc cgggccacgc ttcgaccaga
     1441 tcctgcccag gttgttcccc ggatactcgc gggcgcacct ggccgccgag gtcagggccg
     1501 gtcgcatccg gctggatggc cgccaggtgc cggcacgcac gcgggtccac ggtggcgagg
     1561 aagtcgaatg gcaggacgtc gagcagcccg tggtggggga ccggcccgag gcgatcgaac
     1621 tcgcgatcgt gttcgaggac gccgacctgc tggtgatcga caaacccgcc gggctggtgg
     1681 tgcacccggg cgcgggcaac ccggccggaa ccctgttgaa cgccctgctg caccacgatc
     1741 cggggctggc acggattccg cgcgccggca tcgtgcatcg gctggacaag gacacgtccg
     1801 ggctgctggt ggtggcgcgt tcgctgcgcg cgcacacggc gctgacccgg atgctggccg
     1861 agcgcgacat ccatcgccgc tacgcggcca tcgtcaacgg cgtcccggtc gccggcggca
     1921 cggtggatgc gccgctggac cggcacccgt cggatcgcct gcgccgcgcg gtgcgcgagg
     1981 gcggccggcc ggccgtcacg cactaccggg tgcgcgagaa attccgcgcg cacgcgctcc
     2041 tgcaatgcga gctcgagtcg ggacgcaccc accagatccg ggtgcacatg gcgcacgccg
     2101 ggtacccgct ggtcggggat gcgctgtacg gcaaaggcgt caggctgccg cgcggggcgt
     2161 cgccggccct cgccgacgcc ttgcgcggat tccgccggca ggcgctgcac gccgagcggc
     2221 tggccttcgc gcatcccgtt acggaggagg cgatctcctt cgaagccgaa ccgccgcagg
     2281 atctgctcga cttggccgcg gcgttgcgcg aggatgcgca cgcgccggcg ccgcgccccg
     2341 ggtcctgaat cggcgcaacg gctcgccttt gcacttcggg tgatggaatt cgatcgagca
     2401 gcgtgcgtgc gccccatgtg gatccttccg gactggccaa ggccgccgaa cgtacgcgcc
     2461 gtgacgacga cgcgcgaggg cccctcacca tcccgtttcg gattcaacgt cggcacgcgc
     2521 tgcggggacg aaccgggagc ggtcgccagg aaccgcacgt tcatccgcga ggtcctgcgg
     2581 ttgccttcgg agccgtgctg gttgcggcag gtgcacggca cgcgggtcgt caccctcgat
     2641 accccgccgc agcgcgatga aagcgagggc gacccgatcg tcgagcgcga accggaagcc
     2701 gatgcggccg tcacccgctc gaccggcgtc gtactcgcga tccagaccgc cgattgcctc
     2761 ccggtgctgc tcggcgccga ggacggctgt gaaatcgcgg cggcgcacgc gggttggcgg
     2821 ggactttccg cgggcgtgct ggaaaacacg ctggccgcga tgcgcacgcc gcccgcgagc
     2881 gtgattgcct ggctgggtcc cgcgatcgcg gctgcatcct acgaggtcgg cgaggaggtg
     2941 cgcgacgctt tcctgtcgca cggcgccgcc gccacatcgg cgttcagcgc cacccgtccc
     3001 ggccactgga cctgcgacct gtacgaactc gcccggcagc gcctgcgcgc ggccggcgtc
     3061 acgtgcatcg ccggcggcgg cttcgatacc ttcaaggatc cgcgcctgca ctcgtatcgc
     3121 cgggacggcg ctggaagcgg gcggatggcg agcctcgtct ggattggcgg ctgacgcatc
     3181 tgcggccagt gcaggatgca cgctgccgca tgggtacgat ggcgaacgac gggtccgctg
     3241 gtcagccgtg ttcttccaag gcaccaatga aatcccgccg ccacgcgttg atgtcgtgcg
     3301 tcgacaggtg ctcgaacatt cccgcccaac gttcgcggcg ttcggcgcgt gacatcgtca
     3361 gcccttcctc gatgccttcg accaggcgtt ccgggtcgta gggattgacg aggatcgcct
     3421 gcggcaattc gttggcggcg ccggcgaagc gcgacagcac caggacgccg ggatcgtccg
     3481 gattctgcgc ggcgacgaat tccttggcga ccaggttcat gccgtcgcgc aagggcgtga
     3541 tcagggcgag ctgggagatc cggtagaagc cggccagcgt cgtctgcgcg aagctgcgat
     3601 tcacgtagcg gatcggcacc cagtccggct cggcgtagcg gccgttggtc gcaccggcgg
     3661 agcgttccag tcgcgaacgc agttcgcggt actcggggac ctcctggcgc gaaggcggtg
     3721 cgatctgcag gaacgacacc cgcgaacgcc attcgcggtg ttcggcgagg aactttccga
     3781 acgccgagaa gcgcctgggc agccccttgg aatagtccag ccgatccacg ccgatcgcga
     3841 gcttgcggtc ctcgaggctg gcgcgcaact tgcggatcgc ggagttgccg agcacgctct
     3901 gcgcctggtg cgccacttcg gccgtatcga tgctgatcgg gaagggtgcg atgcgggtat
     3961 ggcgcccatc cagttcgatg acgtcgcccg accggctggt gcccagcacc ccgaggaaat
     4021 agcggaacag cgcgctcgcg tcgtccttga cctgcacgcc gacgaggtcg taggcggcga
     4081 gcgtcgccat caggtccagg tggcaaggca ggttgaccag caactccggc ggcggcagtg
     4141 gaatgtgcag gaagaagccc atgcgcgcac ccaccccgag cgcgcgcagt tcggcggcca
     4201 gcggaatcag gtggtaatcg tggatccaga tgtggtcgtc gtcgcggagc agcggcgcga
     4261 tcttcttcgc gaacagcttg ttgaccgcgc gatagccggc gtattgcccc cggctgtact
     4321 gcatcatggt cggctggaag tgcagcagcg gccacagcgt gcgattggag aagcccaggt
     4381 agtaggcgtc gtattcctcg cgcgtcaggt cgagcagggc gtagtcgatc gagccggcgg
     4441 attcgcgatg cagcgtgcgt tcggattccg cgaccagctt gccgctccac ccgaaccaca
     4501 ggccgccgtg ctctttcagc gcagagcgca tcgcggcggc gagcccgccc gcccgcgttt
     4561 ccctcggctg cgcgacgcga ttggagatga cgacaaggcg cttcacacga catcgctcca
     4621 tttggtggaa aggcccctgg cggcgttgat cagccctacc agcgaatagg tctgcggaaa
     4681 gttgccccac aactcgcgcc gcctggggtc gaggtcttcg gacaacaggc cgagcgggtt
     4741 gcgcatcgcc agcagcttct cgaacagttg gcgcgcctcg ttcttgcggc cgatcgcgat
     4801 caacgcctcg acgtaccaga agttgcagac catgaagctg gtcttgggcg cgccgaagtc
     4861 gtccggctgc gcgtagcgca gcaggaagcc gtcgcgcatc agggtctcgc cgaccgcttc
     4921 cacggtcttg acgaaacgcg ggtcgtcggc gctgacgaag cgcaggcctt cgagcagcag
     4981 caggctggca tcgagatcct cgctgccgaa ggactcgacg aagtagccgt ccttgtggac
     5041 cgcacgtttg agcaccagct cgtggatgcg gtcggcgcgc ttgcgccaga atttcgcacg
     5101 gtccggcagt tgcagccacg tggcgatgtt gcagagccgg tcgcatgcga cccagcacat
     5161 caccgcggaa taggtgtgca ccgattggcg accgcgcaac tcccacagac cggcgtcggg
     5221 cttgtcgtgg agtttccagg ccatctcccc gcagcgttcc agccgccgga attcggtttc
     5281 gtcgccgatc ttgaccagcc gctggtcgaa gaacatctgg gtggccgcca gcaccacgct
     5341 gccgtagatg tcgttctgtt gttgccgcca ggcctcgttg cccacccgca cggggcccat
     5401 gccgcgatat ccggcgagcg tcgccgcttc gcgctccggc aattcgtcct ggaagtggat
     5461 gccatagacc ggcgtcatcg tgtcgttctt gaccgcgagg ttgaagatgt agcggacgta
     5521 ctcttccatg gttcgcgtgg cagacaggcg gttcagcgca cgcaccgaca gcgccgcatc
     5581 gcgcggccag caatagcggt aatcccaggt gcgcaccgta tccggcgctt ccgggatcga
     5641 ggtggtcatc gccgcgacga tcgcgccggt accctcgtac tggcagagct tgagcgtgat
     5701 cgccgcgcgg atcacggcct cctgccactc ggccggaatc gacaggtagc gcgaccattc
     5761 gtgccagtac gcgagtgtcg cgttcagcgc ttcgtcgaaa tagtggcccg ccgattcctg
     5821 cagcgtctca tcggtgccga gcaccatgtg caccggacgg tcgagcagga acggcagctc
     5881 gtcgcgcagc atcggcagtt gcgcgtcact ggtgacccgc agcaccagcg ggtcgagcat
     5941 gaagcgcaca tggttggagc cgaaggtgtg caccggcttg cgcccgccgt aatcggtcag
     6001 cggccgcaac cggatggcga tgcggggcga gcccgacagc ggccgcaccc ggcggaggat
     6061 ctgcatcgga tggtaggttc gttcgtggag cttgtagcgc ggcgcaaagt cggtgatctc
     6121 gatctgggaa ccgtcctcgg cggtgagcac cgtcaccagt accgcgctgt tgtcgacgta
     6181 gcgctgctgg catctggtct cgttctccag ctcgacgtcg aagaacccgg tatctcccag
     6241 cttcggcgaa agcagcgagc agaacaccgg gtcgccatcg aaggccggca cgcagtgcca
     6301 gacgacccgt ccccgggcat cgatcagcgc gttgacggtg ccgttgccga tgattccgag
     6361 atccaggctg gcttcgggcg cgcgat
//
LOCUS       DASSNH010000007         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1334443, whole genome shotgun sequence.
ACCESSION   DASSNH010000007 DASSNH010000000
VERSION     DASSNH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334443"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..2828
                     /locus_tag="VFK00_00270"
     CDS             33..2828
                     /locus_tag="VFK00_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7591423.1"
                     /translation="MKFRKSMLSASIATALLFAAAAQAQDATAQDQTTTDQQQAQTTD
                     QQTSSSSQSDTQQLQAVQVVGIRASLWQSLQTKRAADATVDVVTAEDIGKFPNTNVAE
                     AMTLMPGVTIDRRFGQGERVSINGTDPSLNLSFLDGHPIAQVDWLYGATPDRGFDYSI
                     LAPGMVGQIEVYKTSEARLPSGSLGGTVIIHSRKPLDLKSNTFSASVGEVYNEQAGSG
                     KPNAALFYNWRNDTDTFGFNIGASHYEEKIDRQGREIFGYSPVSAFAANPNVAAQIAD
                     GSIRPTDLMPQEVNAAYFQQNRKRDSVLVNLQLKPNDQWDFGLSGMYIKENFNNWNQS
                     MYGFASQTGATNITSLTSNGSGFITSGHLCGEGDAVNWADGTTSTCGATNTYIDNQVR
                     VSSLVTKDLHLDAGFNGGTWGMQAQVGISKAHNNEAQYFIEPAYHGGYTFDIDTGDHF
                     DDSAAAANPANWGAFGGWFGNAGIAPFTAKTTYGSLDFHVDFNSFINQLLFGVRYVDD
                     KHSEQLRVYGGVNPAQMDQVGDISLTDILDNNSFPGFSPDMRHHIQSSRDNILDWVLS
                     SPLDWNDPYGADPASYINNTWDFAQKTEEAYAQLNFGADALRGNVGVRFVKNKIDSSS
                     YVLGSATPVLPPDPSWWQTDSHSYTDVLPSFNLVYDNGSDVVYRLAGAEVIAWAPYTQ
                     MLGNKFLNDSVLTGSGGNPDLKPYRSLNYSGSVEWYFAPQSVLAFTAFYNHILNYIST
                     ATSTQRNFNSIFDNDPDTYANLYEGKLGNCDANGFCDYQMTIPTGIGPGAVKGVSVSY
                     QQPFGTSGFGLVANYTYALGTTRAGFALPYNSKNSVTVSPYFEKGPFTGRITYNWRSK
                     YLAGGYVAGAAPATVDDYTELDASFGWAFSDQWSVTFAALNLLNESYEMYQDTKAMPL
                     NKYTNGRRYMATLHFKM"
     gene            2932..5235
                     /locus_tag="VFK00_00275"
     CDS             2932..5235
                     /locus_tag="VFK00_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HET7591424.1"
                     /translation="MRRVVCCLLLLVAVPMAGAAAPVAAWSLIPRPAELAPAGQGAVT
                     VRDGDRVLVQAGGDPRALAIVQRFAALVADTRGLRLRVETQADGHSLAAIVFRLDPRA
                     EVDGDAGYRITVGGGRIEVLARAPRGLFYGGVTAWQLLTPDGGRGGAAHVSDGTIVDH
                     PRFQWRGLMLDSARHFQSVADIQKLIDWMSLHKLNVLHWHLTDDQGWRLEVPKYPELT
                     KIGACREAVGPDAALTGSPAKPYCGFYTEADVRAIVHYAADRFITIVPEIDIPGHSQA
                     AIAAYPWLGVTGTRPPVSTDWGINTWLLKPDARTLQFVYDVMDEVMALFPSKYIHIGG
                     DEAAKDQWQASPEVRAHRERLGLKDMDALQGWFTTQVANYLARHGRTAIGWDEILEGK
                     VPESAAVMSWHEPGDALHAIDRGHDVVMASSPTLYLDHYQSDLHDEPPGRPAVESLED
                     VYDYDPVPPAATAAQESHILGVQANLWTEYMPTFARVQHALFPRIAALAEVAWSPAAT
                     HDWRDFLERVPAEIARYRALGIGYADSAFAPAFSLSAGPQGTIEVALSNQADFGTIRY
                     TVDGGAPAASSPLYTKPLSLPADEATEVRATTFAGQFEMAAPRTRRVDEAALLTRNSD
                     QLDTCKNQLVLRLEDDRPLRGTRPVYKIDIENMCWLWKGAPLDGVHGIAITVGDLPWN
                     FQLWTDEAKVVVRPKATPAGELQIHRDSCDGPLLATLPLAQAAHTRLQTTLVADVPPT
                     TGEHDLCIFATGDPRNGMWAIDKIRLK"
BASE COUNT         1040 a         1902 c         1788 g          897 t
ORIGIN      
        1 cccatcactt cgtgagcgag agagggtacg tcatgaaatt ccgcaaaagc atgctttcgg
       61 cgagtatcgc cacggcgctg ttgttcgcgg cggcggcaca ggcgcaggac gccaccgcgc
      121 aggaccagac cacgaccgac cagcagcagg cgcagacgac ggatcagcag accagctcct
      181 cgtcgcaatc ggacacgcag caattgcagg ccgtgcaggt ggtcggcatc cgtgcgagcc
      241 tctggcaatc gttgcagacc aagcgggccg ccgacgccac ggtggacgtg gtcaccgccg
      301 aggacatcgg caagttcccc aacaccaacg tcgccgaggc gatgaccctg atgcccggcg
      361 tcaccatcga ccgccgcttc ggacagggcg aacgcgtcag catcaacgga actgacccga
      421 gcctcaacct gtcgttcctg gacgggcatc cgatcgcgca ggtcgactgg ctgtacggcg
      481 cgacgcccga ccgcggtttc gactattcga tcctcgcgcc cggcatggtc ggacagatcg
      541 aggtctacaa gacctccgaa gcgcgcctcc catcgggcag cctcggcggt accgtgatca
      601 tccacagccg caagccgctg gacctgaagt ccaacacctt cagcgcatcc gtcggcgagg
      661 tgtacaacga gcaggccggc agcggcaagc cgaatgccgc gctgttctac aactggcgta
      721 acgacaccga caccttcggc ttcaacatcg gcgcctcgca ctacgaggaa aagatcgacc
      781 gccagggccg cgagatcttc ggttattcgc cggtgtccgc cttcgcggcc aacccgaacg
      841 tggccgccca gatcgccgac ggaagcatca ggccgaccga cctgatgccg caggaagtca
      901 acgccgcgta tttccagcag aaccgcaagc gcgattcggt gctggtcaat ctccagttga
      961 agccgaacga ccaatgggat ttcgggctga gcggcatgta catcaaggaa aacttcaaca
     1021 actggaacca gtcgatgtac gggttcgcga gccagaccgg cgcgaccaac atcacctcgc
     1081 tgacctcgaa cggttcgggc ttcatcacca gcgggcacct gtgcggcgaa ggtgacgccg
     1141 tcaactgggc cgatggcacc accagcacct gcggcgcgac caacacctac atcgacaacc
     1201 aggtgcgggt ctcgagcctc gtcaccaagg acctgcacct ggatgccggc ttcaacggcg
     1261 gcacctgggg catgcaggcg caggtcggca tcagcaaggc ccacaacaac gaagcgcagt
     1321 acttcatcga accggcctac cacggaggct acacgttcga catcgacacc ggcgaccact
     1381 tcgacgattc cgcggcggcc gccaatccgg ccaactgggg cgcgttcggt ggctggttcg
     1441 gcaacgccgg catcgcgccc ttcaccgcca agaccaccta cggcagcctc gacttccacg
     1501 tcgacttcaa cagcttcatc aaccagctgc tgttcggcgt gcgctacgtc gacgacaagc
     1561 acagcgagca attgcgcgtg tacggcggcg tgaaccccgc ccagatggac caggtcggcg
     1621 acatcagcct taccgacatc ctcgacaaca actcgttccc gggcttctcg cccgacatgc
     1681 gccaccacat ccagtcctcg cgcgacaaca tcctcgactg ggtgctcagc agcccgctcg
     1741 actggaacga tccctacggc gcggaccctg cgagctacat caacaacacc tgggacttcg
     1801 cgcagaaaac cgaagaagcg tatgcccagt tgaacttcgg cgcggacgcg ctgcgcggca
     1861 acgtcggcgt ccgcttcgtc aagaacaaga tcgattcgag ctcctacgtc ctcggcagcg
     1921 ccacccccgt gctgccgccg gatccgagct ggtggcagac cgacagccat tcctacaccg
     1981 acgtgctgcc gtcgttcaac ctcgtctacg acaacggcag cgacgtggtg taccgcctcg
     2041 ccggcgccga ggtgatcgcc tgggcgccgt acacccagat gctcggcaac aagttcctca
     2101 acgacagcgt gctgaccggc agcggcggca atccggacct caagccctac aggtcgctga
     2161 actattcggg ttcggtcgag tggtacttcg cgccgcagtc ggtgctggcg ttcacggcgt
     2221 tctacaacca catcctcaac tacatctcga cagccaccag cacgcaacgc aacttcaact
     2281 cgatcttcga caacgatccg gacacctacg cgaacctgta cgagggcaag ctcggcaact
     2341 gcgatgccaa cggcttctgc gactaccaga tgacgatccc gaccgggatc ggcccgggtg
     2401 cggtcaaggg cgtttcggtg agctaccagc agccgttcgg caccagcggc ttcggtctcg
     2461 tggcgaacta cacctacgcg ctcggcacca cccgcgccgg cttcgcgctg ccgtacaact
     2521 cgaagaactc ggtcacggtc agcccgtatt tcgagaaggg gcctttcacc ggccggatca
     2581 cctacaactg gcgcagcaag tacctggccg gtggctatgt cgcgggtgcg gcgccggcga
     2641 cggtcgacga ttacaccgag ctcgacgcgt ccttcggatg ggccttcagc gaccagtggt
     2701 cggtgacctt cgccgcgctg aacctgttga acgagtccta cgagatgtac caggacacca
     2761 aggcgatgcc gctcaacaag tacaccaacg gccgccgcta catggccacc ctgcacttca
     2821 agatgtaagc cggggcaaga agctctccag cgttcgggcc ggtatggtgt tccggcccgt
     2881 ctttttttca ggagcggggt tttgcggttg gatcgtcagg tgagaagtgc catgcgcagg
     2941 gttgtctgct gcttgctgct cctcgttgcg gtgccgatgg ccggcgccgc ggcgccggtg
     3001 gctgcgtggt cgctgatccc ccggcccgcc gaactggctc ccgcggggca gggcgcggtc
     3061 acggtgcgcg atggcgatcg cgtgctggtg caggccggcg gcgacccgcg ggcgctcgcc
     3121 atcgtgcagc gcttcgcggc gcttgtggca gacacgcgtg gcctgcgcct gcgggtggag
     3181 acgcaagcgg atggccattc gctggctgcc atcgtgttcc ggctcgatcc gcgtgccgag
     3241 gtcgatggcg atgccggcta ccgcatcacg gtcggcggtg gccgcatcga agtcctggcg
     3301 cgtgcgccgc gcggcctgtt ctacggcggc gtcacggcgt ggcaactgct gacgcccgac
     3361 ggcggacgcg gcggggcggc gcatgtttcc gacgggacca tcgtcgacca cccgcgcttc
     3421 cagtggcgcg gcctgatgct cgattccgcg cggcacttcc agagcgttgc agacatccaa
     3481 aagttgatcg actggatgtc gctgcacaag ttgaacgtgc tgcactggca cctgaccgac
     3541 gaccagggat ggcgcctcga ggtcccgaag tatccggagc tgacgaagat cggcgcctgc
     3601 cgcgaggccg tcggaccgga tgcggcgctg acgggctcgc ccgccaagcc gtattgcggt
     3661 ttctataccg aggccgacgt gcgcgcgatc gtgcactacg cggccgaccg cttcatcacg
     3721 atcgtccctg aaatcgacat ccccggacac tcgcaggcgg cgatcgcggc gtatccgtgg
     3781 ctcggcgtca ccggcacgcg tccgccggtg tccaccgact ggggcatcaa cacctggctg
     3841 ctcaaacccg acgcccgcac cctgcagttc gtgtacgacg tcatggacga ggtgatggcg
     3901 ctgttcccgt cgaaatacat ccacatcggc ggcgacgagg cggccaagga ccagtggcag
     3961 gcctcgcccg aagtgcgtgc gcaccgggag cggctcgggt tgaaggacat ggatgcgctg
     4021 cagggctggt tcaccaccca agtcgcgaat tacctcgcgc ggcacgggcg cacggccatc
     4081 ggctgggacg agatcctcga gggcaaggtt ccggaatcgg ccgccgtgat gtcgtggcac
     4141 gaacccgggg atgcattgca cgccatcgat cggggccatg acgtcgtgat ggcttcgtcg
     4201 ccgaccttgt acctcgacca ctaccagtcg gacctgcacg acgagccgcc ggggcggccg
     4261 gcggtggaat cgctcgagga cgtgtacgac tacgatccgg taccgccggc tgcgaccgcg
     4321 gcgcaggaaa gccacatcct cggcgtgcag gcgaacctgt ggaccgagta catgccgact
     4381 ttcgcgcggg tccagcacgc gctgtttccg cgcatcgccg cattggcgga agtcgcgtgg
     4441 tcgcccgccg ccacccatga ttggcgcgat ttcctcgagc gggtgccggc ggaaatcgca
     4501 cgctatcgcg cgctcgggat cggttacgcg gacagtgcgt tcgcgcccgc gttttcgttg
     4561 agtgcagggc cgcaaggaac gatcgaggtc gcgctgtcga accaggccga cttcggcacg
     4621 atccggtaca cggtcgatgg cggcgcgcca gcggccagct cgccgctcta cacgaagccg
     4681 ttgtccttgc ccgccgacga agcgaccgag gttcgcgcga ctacgttcgc cggccaattc
     4741 gagatggcgg cgcctcggac gcggcgcgtg gatgaagccg cactgctgac gcgcaacagc
     4801 gaccagctcg acacctgcaa gaaccagctc gtgttgcggc tggaagacga ccgtccgctg
     4861 cgtggtacgc ggcccgtgta caagatcgac atcgagaaca tgtgctggct gtggaaaggc
     4921 gcgccgctcg acggcgtgca cgggatcgcg atcaccgtcg gcgacctgcc gtggaatttc
     4981 cagttgtgga ccgacgaggc aaaggtcgtg gtgcgtccca aggcaactcc ggccggcgag
     5041 ttgcagatcc atcgcgattc ctgcgatggg ccgctgctgg cgacgctgcc gttggcccaa
     5101 gcggctcata ccagattgca gacgacgctc gtggccgatg tcccgccgac cacgggcgag
     5161 cacgacctgt gcatcttcgc caccggcgat ccccgcaacg gcatgtgggc aatcgacaag
     5221 atccgattga aatagggacg ggcgccgccc gccgcgccgg cgcgttgcgc aggttgggtt
     5281 gatcccgggc tccgcccggg agaagcccaa caatgcgatg ccgcgccaac gttgggcttc
     5341 ctccggataa ctccggagtc aacccaacct acggaaaaag gcgatcgaat cgggtggggc
     5401 ggacgccgtc cgccgcgccg acgcgttgtg taggttgggt tgatcccggg ctcggcccgg
     5461 gagaagcccg acagtgcgat cccgcgccca acgttgggct tcctccggat aaatccggag
     5521 tcaacccaac ctacggaaaa aggcgatcga atcgggtagg acgggcgccg cccgccgccc
     5581 aagcgcgttg cgtaggttgg gttgatcccg ggcttcgccc gggagaa
//
LOCUS       DASSNH010000008         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1258213, whole genome shotgun sequence.
ACCESSION   DASSNH010000008 DASSNH010000000
VERSION     DASSNH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258213"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..2848)
                     /gene="ppc"
                     /locus_tag="VFK00_00280"
     CDS             complement(125..2848)
                     /gene="ppc"
                     /locus_tag="VFK00_00280"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7591425.1"
                     /translation="MSHPHHHPEDRHDLDFAPQDQPLREDVRELGALVGDMLAEQGGG
                     AFFDRVEEIRTAAIRRRVSGAPVDALATILAGSDPAMAGMLARAFATWFQMVNIAERV
                     HRIRRRRDYQRSGAAPQPESLADVLETLKARGVGADELQACIARLDVEPVFTAHPTEA
                     VRRSLLEKEQAIVRALVDRFDTSRTQRERAVDHERLRMALTAGWQTADASGVRPTVQD
                     EFEHIGFYLAEPLYRVLPVFYESFAQELARVYGISGPLPRVLRFGSWVGGDMDGNPNV
                     GADTLGGTLRAQRAMVLERYREGCTSLARLLSQTDDRIDVSDAVRERLAEYRKRLPEA
                     AARIRPRHANMPYRCLLTLMRARLKATGEDASDGYADAGEFECDVALIAESLASHKGA
                     NAGLFAVRRLQWRIRTFGFHLARLDVRQDARVHDDVLAVLLDDAGWRGRDLATRAGRL
                     RPLAAGETEFEGSDDDEVARIRAVFATLADARGRYGSDATGPYIISMARSAADVLAVL
                     ALARHGGLVEDDAVPLDIGPLFETVDDLRHAATTLRALLDDPVYRAHLARRGDRQMIM
                     VGYSDSMKDGGILAARWALQRAQAELLTVAREAGVELTFFHGRGGSACRGGSKVTPAL
                     MASPRGAVAGRLRVTEQGEVIHRKYGIRALALRNLEQTVGATLRASLRPREADAREEA
                     WSRHMDVLASASRDAYREFVGGDGFVDYFRSATPIDVIERMTMGSRPASRRSMHGVED
                     LRAIPWVFSWTQMRSILPGWYGLGTALEKGIAGSGEAAMVEMAREWPFLATLLADVEM
                     MLAKADLGIAEAFSRLSGDLHARFFPRIEQEFRRTRDGILRLKGRDELLADDPRLAQS
                     IRLRNPYVDPMSVLQVDLLRRWREGGSKDGALLRSLVACVNGVARGLQNTG"
     regulatory      2964..3092
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            3105..>3412
                     /locus_tag="VFK00_00285"
     CDS             3105..>3412
                     /locus_tag="VFK00_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7591426.1"
                     /translation="MNAVAQESVKHDYNVRDISLADLGRKRITMAEQEMPGLMQIRAR
                     YAPEKPLKGVRVTGSLHMTKETAVLIETLTALGASVRWASCNIFSTQDDCAAAIAASG
                     V"
BASE COUNT          535 a         1222 c         1143 g          512 t
ORIGIN      
        1 ctgttctcct cccccttcaa gggggaggcc gggaggggga tgggttcgag cgccacttgc
       61 ccgtcctatt gaaacccatc cccacccaac cctccccttg aaggggaggg ctttacctct
      121 gtcctcaccc ggtattctgc agtccgcgcg cgacgccgtt gacgcaggcg acgaggctgc
      181 gcagcagcgc accgtctttg ctgccgcctt cgcgccagcg gcgcagcagg tccacctgca
      241 acacgctcat ggggtcgacg tagggattgc gcaggcggat ggattgcgcg aggcgcgggt
      301 cgtcggcgag cagttcgtcg cggcccttga ggcggaggat cccgtcgcgg gtgcgccgga
      361 attcctgctc gatgcgcggg aagaatcgtg cgtgcaggtc accggacaac cgcgagaatg
      421 cctcggcgat gccgaggtcg gccttggcca gcatcatttc cacgtccgcc aggagggtcg
      481 ccaggaacgg ccattcgcgc gccatctcca ccatcgcggc ctcgccggat ccggcgatgc
      541 ccttttccag cgcggtgccg agcccgtacc agccgggcag gatcgaacgc atctgggtcc
      601 aggagaagac ccaggggatc gcgcgcaggt cttccacgcc gtgcatgctg cgccggctgg
      661 cgggccgcga gcccatggtc atgcgttcga tcacgtcgat gggcgtggcg ctgcggaagt
      721 agtcgacgaa tccgtcgccg cccacgaact cgcggtaggc gtcgcggctg gcgctcgcca
      781 gcacgtccat gtgccgggac cacgcttcct cgcgggcatc cgcctcgcgc ggccgcaggc
      841 tggcgcgcag ggtggcgcct acggtttgct cgagattgcg cagcgccagg gcgcggatac
      901 cgtacttccg gtggatgacc tcgccctgct ccgtgacccg caggcgtccc gccacggcgc
      961 cgcgcggcga ggccatgagc gcaggcgtga ccttgctgcc gccgcggcaa gccgagccgc
     1021 cccggccgtg gaagaacgtg agttcgacgc cggcttcccg cgccacggtg agcagttccg
     1081 cctgcgcgcg ttgcagcgcc cagcgcgcgg cgaggatgcc gccgtccttc atgctgtcgg
     1141 aatagccgac catgatcatc tggcggtcgc cgcggcgcgc gaggtgcgcg cggtacaccg
     1201 gatcgtccag cagcgcgcgc agggtcgtcg cagcgtggcg aaggtcgtcc acggtttcga
     1261 acagcggacc gatgtcgagc ggcaccgcgt cgtcctcgac cagtccgccg tgccgcgcca
     1321 gtgccagcac cgcgagcacg tcggcggccg aacgcgccat ggagatgatg tacggcccgg
     1381 tggcgtcgct gccgtagcgc ccgcgtgcgt ccgccaacgt ggcgaacacc gcgcggatcc
     1441 gcgcgacctc atcgtcgtca ctgccctcga actccgtttc accggccgcc agcggccgca
     1501 ggcgcccggc gcgcgtggca agatcgcgcc ctcgccaccc agcgtcgtcc agcaacaccg
     1561 ccagcacgtc gtcgtgtacc cgcgcatcct gccgcacatc caggcgcgcc aagtggaaac
     1621 cgaaggtgcg gatgcgccat tgcagcctgc gtaccgcgaa caaaccggcg ttggctccct
     1681 tgtgcgaagc gaggctctcg gcgatcagag cgacgtcgca ctcgaactcc ccggcgtcgg
     1741 catagccatc ggacgcgtcc tcgccggtcg ccttcagacg cgcacgcatc aaggtcagca
     1801 ggcagcggta cggcatgttg gcgtggcggg gacggatccg tgcggcggcc tcgggcaggc
     1861 gcttgcggta ttccgccagc cgctcgcgga ccgcatccga cacgtcgatg cggtcatcgg
     1921 tctggctgag cagccgcgcg agtgaggtgc agccctcgcg gtagcgctcg agcaccatcg
     1981 cccgctgggc gcgcagcgta ccgccgaggg tgtcggcgcc gacattggga ttgccgtcca
     2041 tgtcgccgcc cacccacgat ccgaagcgca gcacgcgggg caacggtccg ctgatgccgt
     2101 agacgcgggc caactcctgg gcgaacgatt cgtagaacac cggcagcacg cgatacaacg
     2161 gttcggcgag gtaaaagccg atgtgctcga attcgtcctg caccgtgggc cgtaccccgg
     2221 acgcgtcggc ggtctgccag ccggcggtca gcgccatccg caggcgctcg tggtcgaccg
     2281 cacgctcgcg ctgcgtgcgc gaggtgtcga agcggtccac cagcgcgcgc acgatggcct
     2341 gctccttttc cagcagcgag cggcgcaccg cctcggtggg atgcgcggtg aacaccggtt
     2401 cgacgtccag ccgcgcgatg cacgcctgca gttcgtcggc tccgacgcca cgcgccttca
     2461 gcgtttccag cacgtcggcc agcgactcgg gctgcggtgc ggcgcccgac ctctggtaat
     2521 cccgccgtcg ccggatgcgg tgcacgcgtt cggcgatatt gaccatctgg aaccaggtgg
     2581 cgaaggcacg tgccagcatt ccggccatcg cggggtcgct gccggccagg atggtcgcca
     2641 aggcgtccac cggcgctccg gacacccggc gccggatcgc agcggtgcgg atctcctcga
     2701 cccggtcgaa gaaggcgccg ccgccctgct cggccaacat gtcccccacc agcgccccca
     2761 actcccgcac gtcctcgcgc aagggctggt cctgcggtgc gaaatcgagg tcgtggcggt
     2821 cttcgggatg atggtgggga tggctcacgg ctccctcctc gcgatcaacg tcacacccta
     2881 tcccgattgg gggcgtaacg gcagccggcg gcatgcccgc cacgattcat cttttcatcg
     2941 cgacaaacgg atataatgcc tcgttcgccg aggggcgttg caaccggacg tggatccccg
     3001 cggatccatc cgcgaaaacc gcacatcgag gtgcggacat tcgccagtcc cggtcaggct
     3061 cggccagtcc accgcaacag cgcccatcaa cccggagaaa gtccatgaac gctgttgccc
     3121 aggaatccgt gaaacacgac tacaacgtcc gcgacatctc gctggccgac ctcggccgca
     3181 aacgcatcac gatggccgag caggaaatgc cgggcctgat gcagatccgc gcccgctacg
     3241 cgcccgaaaa gcccctgaaa ggcgtgcgcg tgaccggctc gctgcacatg accaaggaaa
     3301 ccgcggtgct gatcgagacg ctgaccgcgc tcggcgcctc ggtgcgctgg gcgtcgtgca
     3361 acatcttctc gacccaggac gactgcgccg ccgcgatagc cgccagcggc gt
//
LOCUS       DASSNH010000009         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1511174, whole genome shotgun sequence.
ACCESSION   DASSNH010000009 DASSNH010000000
VERSION     DASSNH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511174"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1089)
                     /locus_tag="VFK00_00290"
     CDS             complement(37..1089)
                     /locus_tag="VFK00_00290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7591427.1"
                     /translation="MNMNEDKLHAFLGKAVDDLGAAVSSTLVIVGDRLGLYKALAQGP
                     LDSAELAARTGTNERYVHEWLNNQAAGGYVDYDAGTDKYSLNEEQKLCLADPDGPVDL
                     PGAHYLAESVFHALERTIENFKSGKGMEWGEHHPCLFHGCERFFRAGYNANLLSAWLP
                     ALDGMVDKLRRGGRAADVGCGHGASTILMAKAFPDSEFFGYDYHPASIETARQRAKEA
                     HVENVNFEVVEGTRYKERDLDLVAFFDCLHDMADPVGAARHARRSLKPDGSCMLVEPF
                     ADDAPENNHNPVGRVYYGASAQICVPVSLARKGPALGAQAGEARLRSVMLDGAGFSRM
                     RRAAETPFNLVLEARP"
     gene            1273..2265
                     /locus_tag="VFK00_00295"
     CDS             1273..2265
                     /locus_tag="VFK00_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7591428.1"
                     /translation="MDGVDLLSDVLALVRLTGALIFRLEIHGPWGIAACPTIEKFASL
                     LPAGTNHVLSFHIVLQGECWIRLDAPDWMPVTAGEAVVIARGDPHDVADQPGRATVPL
                     ATMLHGQSVLDLRRASFNTGPGAATAVLCGFLGCDRRAFEPLFNSLPPMFKAGLGPQH
                     RNLVQFALTETLDDRPGAACLRVRLAELLFMGALRAYMQTLPADATGWLAGLRDPLVG
                     RALRTLHAQPCRRWTVDELATAIASSRSVLAERFRNIIGEPPMHYLTRLRMQLAARRL
                     GDSRQSIACVADEVGYDSSAAFQRAFKRCFGVPPAAWRRHAAAGRQPARAASNV"
     gene            complement(2453..>2735)
                     /locus_tag="VFK00_00300"
     CDS             complement(2453..>2735)
                     /locus_tag="VFK00_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7591429.1"
                     /translation="WSGLMSYARHQMPQLGTRGDGLWWMQAFGGHGTAPTCAAGELLA
                     AAIAQNDESWREFAHYGLQSAWRPLGYLGAQAQYWWLEARDRWKAMLEG"
BASE COUNT          419 a          918 c          904 g          494 t
ORIGIN      
        1 acgggtggag gttctccacc ctggttgcgc cgggatctac ggccgcgctt ccagcacgag
       61 attgaacggc gtttcggctg cccggcgcat acgcgagaag ccggcgccat ccagcatcac
      121 cgagcgcagg cgcgcctctc cggcctgggc acccagcgcg gggcccttgc gggccaggga
      181 gaccggcacg caaatctgcg cggacgcacc gtagtacacc cggcccaccg ggttgtggtt
      241 gttctccggc gcatcgtcgg cgaagggctc gaccagcatg caactgccgt ccggcttcaa
      301 tgatcggcgc gcgtgccggg ccgcgccaac cggatcggcc atgtcgtgca ggcaatcgaa
      361 gaacgcgacc aggtcgagat cgcgttcctt gtaccgggtg ccctcgacca cctcgaaatt
      421 cacgttctcg acatgcgcct ctttcgcgcg ctggcgggca gtctcgatcg aggcggggtg
      481 atagtcgtag ccaaagaatt ccgagtccgg gaacgccttg gccatcagga tcgtgctggc
      541 gccgtggccg cagcccacgt cggcggcgcg gccaccgcgt cgcaacttgt cgaccatgcc
      601 gtccagcgcc ggcagccagg ccgagagaag gttggcgttg tagcccgcgc ggaagaaacg
      661 ttcgcaaccg tggaacaggc atggatggtg ttcgccccac tccatgccct ttccgctctt
      721 gaagttctcg atggtgcgtt cgagcgcgtg gaataccgat tcggcgaggt aatgtgcacc
      781 cggcaggtcc acggggccgt ccggatcggc gaggcacagt ttctgttcct cgttgagcga
      841 atatttgtcc gtgccggcgt cgtaatcgac atagccgcct gcggcctggt tgttcagcca
      901 ttcgtgcacg taccgttcat tggtcccggt gcgtgccgcc agttcggcgc tgtccaacgg
      961 gccttgcgcc agggctttgt aaaggcccaa gcgatcgcca acgatcacca gggtcgagct
     1021 gaccgcggcg ccgaggtcgt cgacggcctt gcccaggaat gcatggagct tgtcttcgtt
     1081 catgttcatg gcggttctcc ggaatgttcg acgtgcgttg ttcggcacaa cgcgctggaa
     1141 cgagtggaac accgccgatg cgcgggtgca gtggcatgcc tccagtcgtt ggcctcagta
     1201 ttggcaacgg cgcgcatccc cggcaatgac ctataatccc cgcgagatgc tcgagacacc
     1261 cggaggccgg gcatggatgg agtcgacctg ttgtccgacg tgctggccct ggtgcgtttg
     1321 accggcgcgc tgatttttcg cctcgagatc cacggcccgt ggggcatcgc cgcctgtccc
     1381 accatcgaaa agtttgcgtc gctgctgccg gccggcacca accacgtcct ttccttccac
     1441 atcgtgctgc agggcgaatg ctggatccgg ctcgatgcgc cggattggat gccggttacg
     1501 gcgggcgagg cggtggtcat cgcgcgcggc gacccgcacg atgtcgcgga ccagcccgga
     1561 cgcgcgaccg tgccgctcgc cacgatgctg cacggacaat cggtgctgga cctgcgccgg
     1621 gcgagtttca acaccggtcc gggtgccgcg acggccgtgt tgtgcggttt cctgggttgc
     1681 gatcgccggg ccttcgagcc cttgttcaat tcgctgccgc cgatgttcaa ggcagggctc
     1741 ggtccgcaac atcggaacct ggtgcaattc gcgctcaccg aaacgctcga tgaccgtccc
     1801 ggggccgcct gcctgcgcgt gcgcctcgcc gaattgctgt tcatgggcgc gttgcgcgcc
     1861 tacatgcaga cgttgcccgc cgatgccacg ggctggctgg cgggcctgcg cgatccactg
     1921 gtcggccggg cgctgcggac cctgcatgcg cagccgtgcc ggcgctggac agtggacgag
     1981 ctcgccactg ccatcgccag ctcgcgttcg gtactcgccg agcgcttcag gaacatcatc
     2041 ggcgagccgc ccatgcacta cctgacgcgt ttgcggatgc agctcgcggc acgccgcctc
     2101 ggcgacagcc gccagagcat cgcttgcgtg gcggacgagg tcggctacga ctccagcgcc
     2161 gcgttccagc gcgcgttcaa gcgttgcttc ggggttccgc cggctgcgtg gcggcggcat
     2221 gcggccgcgg gccgccaacc tgcgagagcg gcttccaacg tttgacatcg gtgaaccgga
     2281 acgtcgttgg gcttcgctgc gctcagcgcc aacctaccag agcggcttcc gtaggttggg
     2341 ctgatcccgg acttgttccg ggagaagccc aacgattgaa aacggaaagg gagaaggacc
     2401 tgacgcggtg aaccggaacc atcgctgggc ttcgttgcgc tcagcgccag cctcaccctt
     2461 caagcatcgc cttccagcgg tcgcgggctt ccagccacca gtattgcgcc tgcgcgccca
     2521 agtagccgag cgggcgccat gccgattgca gtccgtagtg ggcgaactcc cgccagcttt
     2581 cgtcgttctg cgcaatcgcg gccgccagca attcgcccgc cgcgcaggtc ggcgcggtgc
     2641 cgtggccgcc gaaggcctgc atccaccaca agccgtcgcc gcgggtgccc agttgcggca
     2701 tctggtggcg cgcgtaactc atcaggcctg accac
//
LOCUS       DASSNH010000010         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1328054, whole genome shotgun sequence.
ACCESSION   DASSNH010000010 DASSNH010000000
VERSION     DASSNH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328054"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1328)
                     /gene="lgt"
                     /locus_tag="VFK00_00305"
     CDS             complement(423..1328)
                     /gene="lgt"
                     /locus_tag="VFK00_00305"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7591430.1"
                     /translation="MSHPFFVDIDPVAFHVGSFGVRWYGLMYLGGFVGALVLGEYRRR
                     RGRLAVDRDGFLDLAFYCMLGIIVGGRIWYMLTYQPINWIWTDPLQLFKVWDGGMSFH
                     GGLLGVLVASLWWWWRHRATVSHYFDVVDFVAPLVPIGLGLGRIGNFINGELWGKISD
                     LPWAMIYPHALQENPRFAGMSVAELRALYPSGVLDAYARQPSELYEFFLEGVVLFTVL
                     FVFSRKPRHRYAVSGLFALLYGCFRFAVEFIREPDPQLGYLFHTGWLTMGQVQSLPLI
                     IVGLVLLWMSRRAPVVRYGAGASPA"
     gene            complement(1451..2656)
                     /locus_tag="VFK00_00310"
     CDS             complement(1451..2656)
                     /locus_tag="VFK00_00310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET7591431.1"
                     /translation="MEEDVEYPALYLKRGEDARLRAGHLWVFANEVDVARSPLQSFEP
                     GQACAIVAAGGKPVGVGYVNPHSLICARLVARGLEHPLDASLLTHRLQVALALRERLY
                     GEPHYRALYGESDGVPGLTLDRFDDVVVAQATTAGIERLKPVIEAAVRKVLKPRALIW
                     KNDAGIRELEGLMAYADVGFGEPPGPLHVREGGLEFAVDAIGGQKTGWFYDQRANRDA
                     LAPFVKGKRVLDMFAYLGAWGLRAAAMGAADVVCVDASASAVRLVGENARRNGLDDRV
                     RADRADAFDYLKTLRERREHFDVVILDPPAFVKRRKDFKQGALAYRRINELAMQVLSR
                     DGILVTCSCSYHMPRAALLDAVQKGARHLDRSLQVLQQLQQAPDHPVHPAIPETDYLK
                     GFICRVLPA"
BASE COUNT          504 a          952 c          838 g          420 t
ORIGIN      
        1 cccatccccc tgctcccccc gaagcgtctt cctgcaaaac cgcgtcacca atgacgcgcc
       61 gaatctccat ggtgttgctg atgcgcccta agaactacgc ccgcatgagc gtggcgtcaa
      121 ccgttgatct tgcggcgcat atgcgcgaac cgcgtgcgcg cgcacgcgta ccatcggaat
      181 ctttcagtaa cctttcattc attccgatat gtagatggcg acgggcacct tgcaggttcg
      241 cgctgagccc agcgacgccc aacgacaagc gcggttcatc gcaacggggc agcattcgac
      301 agctgttggg cttctcccgg agcaagtccg ggatcagccc aacctaccaa agcgtcgacg
      361 tattcttcgt ctgcgcggtc gcatcgcgcg cacgcgtcca gccgcagctg acgactgggt
      421 gcctatgcgg ggcttgcgcc cgccccgtag cgcaccaccg gcgcacgccg cgacatccac
      481 agcagcacca gcccgacgat gatcagcggc agcgattgca cctggcccat ggtgagccag
      541 ccggtgtgga acaggtagcc gagttgcggg tcgggttcgc ggatgaattc gaccgcgaag
      601 cggaagcagc cgtacagcag cgcgaacagt cccgagacgg cgtagcggtg cctcggcttg
      661 cgcgagaaca cgaacagcac cgtgaacagc acgacgcctt ccaggaagaa ttcgtagagt
      721 tcggaaggct ggcgcgcgta ggcgtcgagc acgcccgacg gatacagtgc acgcagctcc
      781 gccacgctca tgcccgcaaa ccttgggttt tcctgcaagg cgtgcggata gatcatcgcc
      841 catggcaggt cgctgatctt gccccacagc tcgccgttga tgaagttgcc gatccgccct
      901 aggccgaggc cgatcggcac cagcggcgcc acgaaatcga ccacgtcgaa atagtggctc
      961 accgtcgcgc gatgccgcca ccaccaccag aggctcgcga ccagcacccc gagcaggccg
     1021 ccgtggaagg acatcccgcc gtcccagacc ttgaacagtt gcagcggatc ggtccagatc
     1081 cagttgatcg gctggtaggt cagcatgtac cagatgcggc cgccgacgat gatgccgagc
     1141 atgcagtaga acgccaggtc gaggaaccca tcacggtcga ccgccagccg gccgcgccgc
     1201 cgccggtact cgccgaggac cagcgcgccg acgaacccgc cgagatacat caacccgtac
     1261 cagcgcaccc cgaacgagcc gacgtggaag gcaacgggat cgatgtcgac gaaaaagggg
     1321 tggctcatcg gctttgctgc cttctccctt cctgggaagg gtcgggaatg gaaattcggt
     1381 ggttcagcga cgcggcatta tgcgcgggat cgcaccctca ccccaaccct cgcccgaagg
     1441 tagagggctc ttatgccggc aacacacggc agatgaagcc cttcaggtaa tcggtttcgg
     1501 ggatcgcggg gtgcaccgga tggtccggcg cctgctgcaa ctgctgcaac acctgcaggc
     1561 tgcgatccag gtgccgcgcg cccttctgca ccgcgtccag cagggccgcg cgcggcatgt
     1621 ggtaggaaca cgaacacgtc acgaggatgc cgtcgcgcga cagcacctgc atcgcgagtt
     1681 cgttgatgcg ccgataggcg agcgcgccct gcttgaagtc cttccggcgc ttgacgaagg
     1741 ccggcggatc gaggatcacc acgtcgaaat gttcgcggcg ctcgcgcagt gtcttcagat
     1801 agtcgaaggc gtccgcgcgg tctgcgcgca cccggtcatc gagcccgttg cggcgcgcgt
     1861 tctcgccgac cagcctgacc gccgacgccg aggcgtccac gcagacgacg tccgccgcgc
     1921 ccatcgccgc ggccctgagg ccccatgcgc cgaggtaggc gaacatgtcc agcacgcgct
     1981 tgcccttcac gaaaggcgcg agtgcgtcgc gattggcgcg ctggtcgtag aaccagccgg
     2041 tcttctggcc gccgatcgcg tccaccgcga attccagccc gccctcgcgc acgtgcagcg
     2101 gccccggcgg ttcaccgaag ccgacgtccg cataggccat caggccctcc agttcccgga
     2161 tgccggcgtc gttcttccag atcagcgcgc gcggtttcag gaccttccgt accgcggctt
     2221 cgatcacggg tttcagccgt tcgatgcccg cggtggtcgc ctgcgccacc accacgtcgt
     2281 cgaaccggtc cagcgtgagt cccggcacgc cgtcggattc gccgtacaag gcgcggtaat
     2341 gcggttcccc gtacaaacgt tcgcgcaatg ccagcgccac ctgcaggcga tgcgtcagca
     2401 aggatgcatc gagcggatgc tccaggcccc gggcaaccag ccgcgcgcag atcagcgaat
     2461 gcgggttgac gtagccgacg ccgaccggct tgccgccggc ggccacgatc gcgcacgcct
     2521 ggcccggctc gaaggactgc aacggcgagc gcgcgacgtc cacctcgttg gcgaacaccc
     2581 agagatgtcc cgcccgaagg cgcgcatcct cgccccgttt caggtacagc gcgggatatt
     2641 cgacgtcctc ttccacgaca ccactccgga ctccaacgca ccacgttatc cgaagcgtgc
     2701 ggaccgccac aagc
//
LOCUS       DASSNH010000011         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_857424, whole genome shotgun sequence.
ACCESSION   DASSNH010000011 DASSNH010000000
VERSION     DASSNH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857424"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFK00_00315"
     CDS             <1..650
                     /locus_tag="VFK00_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591432.1"
                     /translation="GDVNLEDDLGFARRAWSPRLRADFLVGDSQGISIDYYRYRRDRS
                     ASLADSITYNGVTYDAQARARGKLGFDFGSIAYRWWIGKGKDVFGAGLGAGYYKVDAS
                     IAGEASVNGESAQAESSTKASAWAPLLQLGWRHAFNDGWRMYVNASGVRKNGGHLYGH
                     IYNVALGVQWFPTEHFGISAEYDLDRIRLHQRHQTYDDSLDLRLDGPSVFATFRY"
     gene            complement(779..>2729)
                     /locus_tag="VFK00_00320"
     CDS             complement(779..>2729)
                     /locus_tag="VFK00_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HET7591433.1"
                     /translation="GSMHTGLEDRARDFGKLAEYFAARARGGVGLMVTGGFSPNIEGW
                     LKPFSSRLSSRWHVSPHRRITDAVQAAGGKICLQILHAGRYGYHPFSIAPSRVKSPIT
                     PFTPRALSARGIDRTIGAFARCARLARDAGYDGVEIMGSEGYLINQFLAERSNRRSDR
                     WGGSPENRMRFPVEIVRRTRQTVGRDFIIIYRLSMLDLVDRAQAWDEIASLAKQVEAA
                     GATLINTGIGWHESRIPTIVTSVPRAAFTWVTRRMKESVRIPLITTNRINLPEVAEAV
                     LAGGDADMVSMARPLLADPDWVAKARTGRSALINPCIACNQACLDHVFENKRASCLMN
                     PRACHETELVDAPAVQKKHFAVVGAGPAGLACATTLAQRGHAVTLFEQASEIGGQFNL
                     ARRIPGKEEFAEPLRYWGNLLAELGAEVRLGTRADATMLQAQGFDAVVVATGVTPRDP
                     RIPGQEHSMVLGYHDAVMGRRGIGRRVAIIGAGGIGFDVAEFLTQAAPSPTTDIARWT
                     REWGVDTSLESRGGLARPAPEPSPREVWLLQRTAGRPGRRLNKTTGWVHRAALKAKGV
                     KMIGGVTYESIDDAGLHVTIDGKRQVIEVDNVVICVGQESNRSLADALAGLGITVHVI
                     GGADVAAELDAKRAIRQATELAATL"
BASE COUNT          442 a          929 c          905 g          453 t
ORIGIN      
        1 gcggcgacgt caacctcgaa gacgatctcg ggttcgccag gcgcgcctgg tcgccgcgcc
       61 tgcgcgcgga cttcctggtc ggtgacagcc agggaatctc gatcgactac taccgctatc
      121 gccgtgatcg cagcgcgtca ctggccgaca gcatcacgta caacggcgtc acctacgacg
      181 cgcaggcacg cgcgcgcggc aagctcggct tcgatttcgg cagcatcgcc taccgctggt
      241 ggatcggcaa gggcaaggac gtgttcggcg cgggcctcgg cgccggctat tacaaggtgg
      301 acgcctcgat cgcgggcgaa gcttcggtga acggcgaatc cgcgcaagcc gaatcctcga
      361 cgaaggcgag cgcctgggcg ccgctcctgc aactcggctg gcgacacgcc ttcaacgacg
      421 gctggcggat gtacgtgaac gcgtccgggg tcaggaagaa cggtgggcat ctgtacgggc
      481 acatctacaa tgtcgcgctg ggcgtgcagt ggttcccgac cgagcatttc ggcatcagcg
      541 ccgaatacga cctcgaccgc atccgcctgc accagcgcca ccagacctac gacgacagcc
      601 tcgacctcag gctggacggc ccctcggtat tcgcgacgtt ccggtattag gggatccgcg
      661 gtcatcgccc ccggacgcgc tttgcgtagg agcgcgccat gcgcgcgacc cggtcctcgc
      721 ggtcgtgcac ccttgtcgcg cgcggaagaa ggagcaagcg cttcgcgcag gcccgctcct
      781 acaacgtcgc cgcgagctcc gttgcttgcc ggatggcgcg cttggcgtcg agttcggcgg
      841 cgacgtcggc cccgccgatc acgtgcacgg tgatgccgag gccggccagt gcgtccgcca
      901 gcgaacgatt cgattcctgg cctacacaga tcacgacgtt gtcgacttcg atgacctgcc
      961 gcttgccgtc gatcgtgacg tgcagcccgg catcgtcgat gctttcataa gtgacacccc
     1021 cgatcatctt cacgcccttg gctttcaggg cagcacggtg cacccaaccg gtggtcttgt
     1081 tgaggcgccg gcccgggcgc ccagccgtgc gttgcagcag ccatacttcg cgcggcgaag
     1141 gttcgggtgc cggcctggcg aggccgccgc gcgattcgag gctggtgtcc acgccccatt
     1201 cgcgggtcca gcgcgcgatg tcggtggtgg gcgagggcgc agcttgcgtc aggaattccg
     1261 cgacatcgaa accgatgccg cccgcgccga tgatcgcgac gcgcctgcca atcccgcgcc
     1321 tgcccatgac agcatcgtga tagcccagca ccatcgagtg ttcctggccc gggatgcgcg
     1381 gatcgcgcgg cgtgacgccg gtcgcgacga ccacggcatc gaagccctgg gcctgcagca
     1441 tcgtcgcatc ggcacgtgtt ccgagacgga cctccgcgcc gagctccgcc agcaggttgc
     1501 cccagtaacg cagcggctcg gcgaactcct ccttgcccgg aatgcgccgc gcgagattga
     1561 actggccgcc gatttcgctt gcctgctcga acaaggtcac cgcatgcccg cgttgcgcga
     1621 gcgtcgtggc gcacgccagt cccgccggcc ccgcgccgac caccgcgaaa tgcttcttct
     1681 gcacggccgg cgcatcgacc aattcggttt cgtggcacgc gcgcggattc atgaggcagg
     1741 aggcgcgctt gttctcgaac acgtggtcga ggcaagcctg gttgcaggcg atgcagggat
     1801 tgatcagtgc gctgcggccg gtgcgtgcct tcgcgaccca atcgggatcg gccagcaatg
     1861 gacgcgccat cgacaccatg tcggcatcgc cgccggccag cacggcttcg gcgacttcgg
     1921 gcaggttgat gcggttggtg gtgatcagcg ggatccgtac cgattccttc atccgccgcg
     1981 tcacccaggt gaacgcggcg cgcggcacgc tggtgacgat ggtcgggatc cgcgactcgt
     2041 gccagccaat cccggtgttg atcaatgtcg cacccgcggc ttcgacctgt ttcgcgagcg
     2101 aggcgatttc gtcccaggct tgcgcgcgat ccaccaggtc cagcatcgac agccggtaga
     2161 tgatgatgaa gtcgcgtccc accgtttggc gggtgcggcg cacgatttcg accggaaagc
     2221 gcatccggtt ttcgggcgaa ccgccccagc ggtcgctgcg ccggttgctg cgctcggcaa
     2281 ggaactggtt gatcaggtaa ccctcgctgc ccatgatttc gacgccgtcg tagccggcgt
     2341 cccgcgcgag cctggcgcaa cgcgcaaagg caccgatcgt ccggtcgatg ccgcgcgcgg
     2401 acagcgcccg cggggtgaag ggcgtgatcg gcgacttgac tcgcgacggc gcgatggaaa
     2461 acggatggta gccatagcgt cccgcgtgca ggatctgcag gcagatcttt ccgcccgccg
     2521 cttgcacggc atcggtgatg cggcgatgcg gcgaaacgtg ccagcgggaa gacaaccggc
     2581 tcgagaaggg tttcagccaa ccctcgatgt tcggcgagaa accgcccgtc accatcaagc
     2641 cgacgccgcc gcgggcgcgc gcggcgaagt actcggcaag tttcccgaaa tcgcgcgcgc
     2701 gatcttcgag gcccgtgtgc atcgatccc
//
LOCUS       DASSNH010000012         7016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_855740, whole genome shotgun sequence.
ACCESSION   DASSNH010000012 DASSNH010000000
VERSION     DASSNH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855740"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFK00_00325"
     CDS             <1..615
                     /locus_tag="VFK00_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HET7591434.1"
                     /translation="FKNRFGAVNELGVFAMGDKGLREVPNPSSIFLTGHQGATPGSAV
                     MVTREGTRPLLVEVQALVDQSNLGNPRRVALGLEQNRLAMLLAVLHRHGGAAVFDQDV
                     FVNVVGGIRVQETAADLPVLLAVLSSFRDRALPDKTVAFGEVGLSGEVRPVPNGEERL
                     KEAAHHGFRRAIVPQANAPKKSTRIGDLEVVGVERLGDAIDAVA"
     gene            complement(801..1205)
                     /locus_tag="VFK00_00330"
     CDS             complement(801..1205)
                     /locus_tag="VFK00_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7591435.1"
                     /translation="MLIRLLGAMLAGAVLGWQRERSDKPAGLRTHILVALGAAIFVVV
                     PEQLAMNPDALSRVIQGIATGIGFLGGGVILKHASERRIEGLTTAADIWATAAIGVAI
                     GLGALGIGLIATMLAWIVLSIVYRLQARGDHR"
     gene            complement(1262..2011)
                     /gene="lpxH"
                     /locus_tag="VFK00_00335"
     CDS             complement(1262..2011)
                     /gene="lpxH"
                     /locus_tag="VFK00_00335"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET7591436.1"
                     /translation="MATLFVSDLHLDDARPRIVEQFERFCAGEARTADALYVLGDLFE
                     AYVGDDDDAALNTRIANALRGVADTGVPVHYIAGNRDFLVGADYARRCGMDILADGTV
                     VDLHGTPTLILHGDVLCTDDAAYQAFRSQVRDPAWQAAFLARPLGERRAFAAQARDAS
                     RAHTSTTDMLIMDVNQDAVERALGEAGVERMIHGHTHRPAIHDFLLDEKPAQRIVLGD
                     WYEQGSVLRIDAGGAALQLLRPQRTDPGPPP"
     gene            complement(2016..2738)
                     /locus_tag="VFK00_00340"
     CDS             complement(2016..2738)
                     /locus_tag="VFK00_00340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7591437.1"
                     /translation="MKDRRHPFRWLLPAAFALALPALAPAAPPASAASTAAPTGPVHP
                     QVAIKTSMGDIVIELFPDKAPKTVANFLQYVKDGFYAGTVFHRVIPGFLVQGGLYTRE
                     LRPRRTLPPIPDEADNGLSNLRGTVAAARGPDPDSATAQFFINVVDNPRLDYVGNQSG
                     MTWGYAVFGKVVKGMDVVDKIDNLPTGPQGPFIGDVPRPLVVIEGAKLIGEAANPIPE
                     PVSPVTGQAPASAPAAKKPAKH"
     gene            complement(2809..4569)
                     /locus_tag="VFK00_00345"
     CDS             complement(2809..4569)
                     /locus_tag="VFK00_00345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7591438.1"
                     /translation="MPLRFRPGTLLSLAATLALALPAAAQQADVRLPSLGSSADSVIS
                     PQEAEQYGAEFLRELRAADLVLDDPQVDQYLQTLGYRLASASADPSQHYTFTLINVPQ
                     INSFATFGGYIFIFSGMVTATQSQDELAAVMAHELAHVSQQHLQRAVEDQKKSLPLYV
                     LGTLAAALAASRMDDSRPVSTAPYGYGYGNNTPYGGGVDPVQGVIAAGMGLMAQHQIE
                     FTRKDEEEADHVGIDTLAKAGFDPEGMADVFAHMQALTRPGGDGGMAAGNAPDFLQDH
                     PVSSVRIADARARARVLDQQMKSRKTGAAGDPLGPGLVPLPFVNGGAVVLGDPSQGPS
                     ELEYQLMRERIRVLSSANPRSTLDYYRRSMAGSQKFAANPANRYGYALALVQLGEAGK
                     AVPVLESLVEARPHDLTLALGLADAERRAGQRDQALQRYASLNAMWPNDSAIVLDYSR
                     ALLSDGSKASAKTAQGLLKPLLDDDSEPSLYETYGHACHVAGDDVHAGIAYADATFLS
                     GRAADALDQLHRLLDRKDLDYYTRAQIDARIAQVTPIVLEIRRRHLDRGDDYGNQLQG
                     SELRAGFRFGACAGSECLQR"
     gene            4730..4993
                     /gene="grxC"
                     /locus_tag="VFK00_00350"
     CDS             4730..4993
                     /gene="grxC"
                     /locus_tag="VFK00_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HET7591439.1"
                     /translation="MPDIEIYSTAICPYCNAAKSLLRSKGLEWRELRIDLDPEARRAM
                     AERTQGARTVPQIFINGTLVGGFDQLAAAERSGKLNELLQVAG"
     gene            5313..6323
                     /locus_tag="VFK00_00355"
     CDS             5313..6323
                     /locus_tag="VFK00_00355"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HET7591440.1"
                     /translation="MSKTIAVIRGDGIGPEIMDATLKVLDALDCGLDYEFSEAGMAAL
                     EKHGELLPQATLDAIARHRVALKGPLTTPIGGGYSSLNVALRRHFDLYANVRPAISFP
                     GTKARYENIDIITVRENTEGAYLAEGQTLSEDGEVAQSLVRNTRTGASRIVRHAFELA
                     ARKGRKKITAVHKANIIKTASGLFLDVARGISKEYPQIAFNEMIVDNTCMQLVMNPWQ
                     FDVIVTTNLFGDIISDLCAGLVGGLGLAPGANIGKDVAIFEAVHGSAPDIAGKNIADP
                     CALLLAAAEMLDHLDMTAKATRIRNAIRATVEAHDRVTPDLGGNGTTTTFAEALVECV
                     RA"
     gene            complement(6426..>7016)
                     /locus_tag="VFK00_00360"
     CDS             complement(6426..>7016)
                     /locus_tag="VFK00_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET7591441.1"
                     /translation="YVKWYGEPLPSAQKLCPKTVELVNRIPRVKAAMFALLPPGATLN
                     PHRDPFAGSLRYHMGLMTPNSDACHIVVDGQDYSWRDGEDVIFDETCVHWAENKTDTP
                     RVILMCDLERPLRSRLMAAINHHVSNFMGAITASPNQASEPTGAINRLFSALRQRREK
                     NRGFKQRHRALFKTMKFVGSVALLWVLLLAPWPLLR"
BASE COUNT         1141 a         2299 c         2444 g         1132 t
ORIGIN      
        1 ttcaagaacc gcttcggcgc ggtcaacgaa cttggcgtgt tcgcgatggg cgacaagggc
       61 cttcgcgagg taccgaatcc gtcgtcgatt ttcctcaccg gccatcaggg tgcgacgccc
      121 gggagcgcgg tgatggtgac ccgcgaaggc acccggccgt tgctggtcga agtgcaggcg
      181 ctggtcgatc aatcgaacct cggcaatccg cgccgggtgg cgctggggct cgaacagaac
      241 cgcctcgcga tgctgctggc ggtgctgcat cgccacggcg gcgccgcggt gttcgaccag
      301 gacgtgttcg tcaacgtggt gggcggcatc cgtgtgcagg aaaccgccgc cgacctgccg
      361 gtgctgctcg cggttttgtc gtcgtttcgc gatcgggcat tgccggacaa gaccgtcgcg
      421 ttcggcgaag tcggcttgtc gggcgaagta cgcccggtgc cgaacggcga ggaacggctg
      481 aaggaagccg cgcaccacgg attccgccgc gcaatcgtgc cgcaggccaa cgcgccgaag
      541 aaatcgaccc gcatcggcga tctcgaagtc gtcggtgtgg agcgcctggg cgacgcgatc
      601 gacgcggttg cgtagcctga ggcgactgcg tttgcaggac ggcaagcgcg ccggcgaagt
      661 tccgaagggc gagcgccaag gacggccgag tcgcccgaag ggcgagtgcc atggagccgc
      721 gaatcaatcc ggggctatgg ttcgcggaaa gcttcccgga ttccggcgcc gcgcgtctgc
      781 atccgggctg cgatgctgcg tcagcgatga tctccccgcg cctgaagccg atatacgatg
      841 ctcagcacga tccaggcaag catcgttgcg atcaggccga tgccgagcgc gcccagcccg
      901 atcgcaacgc cgatcgcggc ggtggcccag atgtccgcag cggtggtcaa cccttcgatc
      961 ctgcgttcag aggcgtgctt caggatcacg ccgccaccaa ggaatccgat cccggtggcg
     1021 atgccctgga tcacgcgcga cagcgcgtcc gggttcattg cgagctgctc gggcacgacc
     1081 acgaagatgg cggcgccgag agcgacgagg atatgggtgc gcaaccccgc cggcttgtcc
     1141 gaccgttcgc gttgccagcc gagcaccgcg ccggccagca tggcgccgag cagccggatc
     1201 agcacccgca gcaatgcggt gccatcggga aggccgccgc tgagttcgtc gatcagcacg
     1261 ttcaaggcgg cggccccggg tccgtccgtt gcggtcgcag gagttgcaag gccgcgccgc
     1321 ctgcatcgat gcggagaacc gacccctgct cgtaccagtc acccagcacg atccgctgcg
     1381 cgggtttttc gtcaagcagg aaatcatgga tggcggggcg atgggtatgg ccgtggatca
     1441 tccgctcgac gccggcttcg ccgagcgcgc gttcgaccgc atcctggttg acgtccatga
     1501 tcagcatgtc ggtagtcgag gtgtgcgcac ggctggcatc gcgcgcctgt gcagcgaacg
     1561 cgcgccgctc gcccaacggc cgtgcgagga acgctgcctg ccacgcggga tcgcggacct
     1621 ggctgcggaa cgcctggtat gccgcatcgt cggtacacag cacgtcgcca tgcaggatca
     1681 gcgtgggcgt gccgtgcagg tcgaccaccg ttccgtcggc gaggatgtcc atcccgcaac
     1741 gccgtgcgta atccgcgccc acgaggaaat cgcggttgcc cgcgatgtag tggaccggca
     1801 cgccggtgtc cgccacgccg cgcaacgcgt tcgcgatgcg ggtgttcaat gcggcatcgt
     1861 cgtcgtcgcc gacgtaggcc tcgaacaggt cgccgagcac gtacagcgca tccgcggtgc
     1921 gcgcctcgcc tgcgcagaat cgctcgaact gctcgacgat ccgggggcgc gcgtcgtcga
     1981 ggtgcaggtc ggagacgaag agggtcgcca ctgcgtcagt gcttggcggg cttcttcgcg
     2041 gcgggcgccg aagccggcgc ttgtcctgtc accggcgata cgggttccgg gatcgggttc
     2101 gcggcctcgc cgatcagctt ggcgccttcg atcaccacca gtgggcgcgg cacgtcgccg
     2161 atgaacggcc cctgcggccc ggtcggcagg ttgtcgatct tgtcgaccac gtccatgccc
     2221 ttgacgactt tgccgaacac cgcataaccc caggtcatgc cgctctggtt gcccacgtaa
     2281 tcgagccgcg ggttgtcgac cacgttgatg aagaactgcg ccgtggcgga atcggggtcg
     2341 gggccgcgcg ccgcggccac ggtaccacgc aggttcgaga gcccgttgtc ggcttcgtcg
     2401 gggatcggcg gcagcgtgcg cctgggccgc agttcgcggg tgtacagccc gccttgcacc
     2461 aggaagccgg gaatcacgcg gtggaatacg gtgcctgcgt aaaagccgtc cttcacgtac
     2521 tgcaggaagt tggcgaccgt cttcggcgcc ttgtcgggga acagctcgat cacgatgtcg
     2581 cccatcgagg tcttgattgc cacctgtggg tgcacgggcc cggtgggcgc ggccgtcgag
     2641 gcagcggaag ccgggggcgc ggcgggggcg agcgcgggca acgcgagcgc gaaagcggcg
     2701 ggcagcaacc agcggaacgg gtgtcggcgg tctttcatgg gcagcgcgca atccggtgtc
     2761 gaccgcattc tacgggagcg gcgcggtatc cacggaccat gcgcccgctc aacgctgcag
     2821 gcattcgctg ccggcgcagg cgccgaaacg gaatcccgcc cgcaactcgc tgccctgcaa
     2881 ctggttgccg taatcgtcgc cgcggtcgag atggcggcgc cggatctcca gcacgatcgg
     2941 ggtgacctgg gcgatccggg catcgatctg cgcgcgggtg tagtagtcga ggtccttgcg
     3001 atcgagcagg cggtgcaact ggtcgagcgc gtcggcggcc cggcccgaca ggaacgtggc
     3061 gtcggcgtag gcaatgccgg catgcacgtc gtcgcccgcc acgtggcagg catggccgta
     3121 ggtctcgtac agcgacggct cggagtcatc gtcgagcaac ggcttcagca ggccctgcgc
     3181 ggtcttcgcg ctggccttgc tgccgtcgct tagcagcgca cggctgtaat cgagcacgat
     3241 cgccgaatcg ttcggccaca ttgcgttcag cgaggcgtag cgttgcagcg cctggtcgcg
     3301 ctgccccgcg cggcgctcgg cgtcggccag gcccagggcg agggtgaggt cgtgcggccg
     3361 cgcctcgacc agcgattcaa gcaccgggac cgccttgccg gcctcgccca gttgcaccag
     3421 cgccagcgcg tagccatagc ggttggcggg gtttgccgcg aacttctggc tgccggccat
     3481 gctgcgccgg tagtagtcca gcgtgctgcg cggattggcg gaggacagca cccggatgcg
     3541 ttcgcgcatc agttggtact cgagttcgct cgggccctgg gacggatcgc cgagcaccac
     3601 ggcgcccccg ttcacgaacg gcaagggcac gaggcccggg cccagcgggt cgccggcggc
     3661 tccggtcttg cgcgacttca tctgctggtc gagcacccgc gcgcgggcac gcgcatcggc
     3721 gatgcgcacg ctgctgaccg ggtggtcctg gaggaaatcc ggggcgttgc cggccgccat
     3781 gccgccgtcg ccgccgggac gggtcagcgc ctgcatgtgc gcgaacacgt cggccatgcc
     3841 ttcggggtcg aagccggcct tggccagcgt atcgatgccc acgtggtcgg cttcctcctc
     3901 gtccttgcgg gtgaattcga tctggtgctg cgccatcagt cccatgcccg cggcgatgac
     3961 gccctggacc gggtcgacgc cgccgccata gggcgtgttg ttgccgtagc cgtagccgta
     4021 tggcgccgtc gataccgggc gcgagtcatc catgcgggag gcggcgaggg ccgccgcgag
     4081 ggtgccgagc acgtacagcg gcagcgactt cttctggtcc tccacggcgc gctgcaggtg
     4141 ctgctggctg acgtgggcga gctcgtgcgc catcaccgcc gccagttcgt cctgcgactg
     4201 ggtagcggtc accatgcccg agaagatgaa gatgtagccg ccgaaggtcg cgaacgagtt
     4261 gatctgcggg acgttgatca aggtgaacgt gtagtgctgg ctgggatcgg cactcgccga
     4321 cgccaggcgg tagccgaggg tctgcaggta ctggtccacc tgcgggtcat ccagcaccag
     4381 gtcggccgcg cgcagttcgc gcaggaattc ggcgccgtac tgctcggctt cctgcggcga
     4441 gatcacggag tcggcggaac tgcccaggct cggcaggcgc acgtcggcct gctgggcggc
     4501 agccggcagc gcgagcgcga gcgtcgcggc gagggacagc agggttccgg gacggaaacg
     4561 aagtggcatg gagcgtgcgg gtgcggggtc tgacattgga ccggccgtgc ggcggttggt
     4621 tcgccgcgct ttcgtcccaa gatagagggt gcgcgttcgc gaagccagtc gcagcggcag
     4681 cgcctcggtc gccctagaat cgcccggaat tcaacgatcc cctcccgcca tgcccgacat
     4741 cgaaatctac tctaccgcca tctgtcccta ctgcaacgcc gccaagagcc tcctgaggtc
     4801 gaagggactg gaatggcgcg agctgcggat cgacctcgac cccgaggcgc gcagggcgat
     4861 ggccgaacgc acccagggtg cccgcacggt gccgcagatc ttcatcaacg gaacgctggt
     4921 cggcggcttc gaccagttgg ccgcggccga gcgcagcggc aagctgaacg aattgctcca
     4981 ggtcgccggg taaacggcca cactggccaa cgctccatcc tcgtagctgg cttcgcacgc
     5041 gggatgagcg cgaagcgttc gaagggtggc agtcctcctt gggccaccgg catcgcgcgg
     5101 tcggttgctt ggtttccgct tgcccgatcg gccataattc cgctcttgtt tgttcgctcc
     5161 gtcgcgtcct acgcgcccaa cggagcggct tttgtgaaaa gtcaggccat ggatggccgt
     5221 tctggactcc cgtgtctcat gtcgtctgca ctggccgcaa gaaccggcga agcagcgatc
     5281 agggacgatc gcaaaaacaa aggagctgct gaatgagcaa gacgattgcg gtgatccgtg
     5341 gcgacggcat cggtccggag atcatggacg ccacgctgaa ggtcctggat gcgctggatt
     5401 gcggcctcga ctacgagttc agcgaggccg gcatggcggc gctggaaaaa cacggcgagt
     5461 tgctgccgca ggccacgctc gacgcgatcg cgaggcaccg ggtcgcgctg aaagggccgc
     5521 tgacgacacc gatcggcggc ggctacagct cactgaacgt ggcgctgcgc cggcacttcg
     5581 acctgtacgc caacgtgcgc cccgcgatca gctttccggg caccaaggcg cgctacgaga
     5641 acatcgacat catcaccgtg cgcgagaaca ccgagggtgc gtatctcgcc gagggccaga
     5701 cgctgtccga ggacggcgag gtcgcccagt cgctggtgcg caacacccgc acgggcgcca
     5761 gccgcatcgt gcgccacgcc ttcgagctgg cggcgcgcaa ggggcggaag aagatcacgg
     5821 cggtgcacaa ggccaacatc atcaagaccg cctcgggcct gttcctcgat gtcgcgcgcg
     5881 ggatctcgaa ggagtacccg cagatcgcgt tcaacgagat gatcgtcgac aacacctgca
     5941 tgcagctcgt gatgaatccg tggcagttcg acgtgatcgt gaccaccaac ctgttcggcg
     6001 acatcatttc ggacctgtgc gccggattgg tcggcggtct tggcctcgct cccggcgcca
     6061 acatcggcaa ggacgtcgcg atcttcgagg cggtgcatgg atcggccccg gacatcgcgg
     6121 gcaagaacat cgccgatccc tgcgcgctgc tgctggccgc cgccgagatg ctcgaccacc
     6181 tcgacatgac cgcgaaggcc acgcgcatcc gcaacgccat ccgcgcaacc gtggaagcgc
     6241 acgatcgcgt caccccggat ctcggcggca acggcaccac cacgaccttc gccgaagcgt
     6301 tggtcgaatg cgtacgcgcg taactcgaat tcggggcgcc ggcggaaacc gctacaggag
     6361 ttccgcccca cgttgcttta gcactccccg ccaaggcaag gggaagagcg ggaaggcgcc
     6421 ttgcttcacc gcaacaacgg ccagggcgcc agcagcagca cccacagcag cgccacgctg
     6481 cccacgaact tcatggtctt gaacagcgcc cggtggcgct gcttgaagcc gcggttcttc
     6541 tcgcggcgct ggcgcagtgc cgagaacagg cggttgatcg cccccgtggg ttcgctggcc
     6601 tggttcggcg aagcggtgat tgcgcccatg aaattgctga cgtggtggtt gatcgccgcc
     6661 atcagccgcg aacgcagcgg ccgctcgagg tcgcacatca ggatcacgcg cggggtgtcg
     6721 gtcttgttct ccgcccagtg cacgcaggtc tcgtcgaaga tcacgtcttc gccgtcgcgc
     6781 caggaataat cctggccgtc caccacgatg tggcaggcat cggagttggg cgtcataagg
     6841 cccatgtggt agcgcaggga ccccgcgaac ggatcgcgat gcgggttgag ggtggcgccg
     6901 ggcggaagca gggcgaacat cgcggccttc acgcgcggga tccggttcac cagttccacc
     6961 gtcttcgggc agagcttctg cgccgacggc agcggttcgc cgtaccactt cacgta
//
LOCUS       DASSNH010000013         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_244584, whole genome shotgun sequence.
ACCESSION   DASSNH010000013 DASSNH010000000
VERSION     DASSNH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244584"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..>3936
                     /locus_tag="VFK00_00365"
     CDS             394..>3936
                     /locus_tag="VFK00_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7591442.1"
                     /translation="MTRRPARGFLAGAFLVLLVCCGAGSPALAGEPEVSFDIPAQPLA
                     SALLQFSDQAGAQIASNSVDVSNSYSHGVQGQMPVEAALKRLLEGTGFSFVALPENTF
                     AVRRGSGRAAGSDPPPSATHAFDIPGGPLSDSLDRFAAQSGLRVEYDHELVRGHSARP
                     YQNTAPSLVVLEDLLAGSGLTYRVSAPGTVIIARPETTAAAPTSAALPAPGAQGTQLA
                     TITVTGSRIKRPGYDTLEATSVIDSAQIENRGYTNALQALQATPGFGAPSSSPISAAQ
                     GRMNIGQSFANFFGLGSQRTLTLVNGQRFVSSNPVGTNSANASPGSQVDLNLIPIDLI
                     DRIETVAIGGAPVYGSDAIAGTVNIILKDNFEGVAATAQYGISEKGDAQNKMASVLMG
                     GNFASNRGNAVISAEYVHQDPLVFSDRYGLSYALPNPAYTGPGEGIPAVLFYPDVHFD
                     FMTEGGMPYDGSLLDIPGLHYPGLYPNGNYIFDSSGQPMRFAPDGELVPLDFGTVVNS
                     ASLGGGISVPLYSTGGDGVNAADHFGLLAGSTRKIFNGIAHFDLTDNVRAFINTEYAH
                     TSSVLPSDVTSLIAPGLFPSPSLTFSVDNPFLSDQARDIIEANGLTSFNLARNLNDLV
                     DRNPATLDLNVSRVVLGLKGNFDAFGNQWSWDVSYNNGTSRSVSTDTYINPDRLVLAA
                     DAVRDASGEIVCASGGDCVPIDLFGENAFSSAAAAYVSDRVATTARNSMNDWQANISG
                     GLPFEIAREEPVKISFGFEHRAESGSTQPDAGAKLGDTLVGVPGFTAISGSYSINAFY
                     GETLIPLVSDAQRVSWVKAASVDLAARHVDNSINGGALTWSAGGRFQPRFPGLGDGLV
                     FRGVFMHAIRSPSITELFLPSSGSLGGIVDPCDAGNYTSGPNPAARTANCEAALAAAG
                     APQPGEFHSTTRSISVAGLRTGNPNLKNETADSWSVGMVYQPVEFPRFRMSLDWNHIK
                     LKDGIVSLGIDDILASCYDSSNYPDNDACALFSRLTAAEASTPRVPGDVAPGYKQGFV
                     NSSGADLSGAILAAEYVQPLPHDAGALNFSGSVFYRNHYNVVDFEGGSETHAAGTIAY
                     PKVQATLSAGYSWRRFETVWQARWTSGVVIDNTATIEDYPAYFISPYTLVSATFGYKF
                     TPGVSLRLIVNNVFDKKLPDVALEQRAFSLYDPIG"
BASE COUNT          700 a         1304 c         1207 g          725 t
ORIGIN      
        1 caccgactcg gaacgggcgg cttcatcgcg gtccgcgcgc ggctccgccc atcgcggcga
       61 ctctggcgca tcttcgagca gttgataggg caatgcccgt ctccccgagc cggatgactg
      121 gtgcggcagc cagcggtcca accacggccg ccatcatata ggacgttctg cgcgcgacaa
      181 gcggaaatgg ccgcgttcca ccatccgcgc ggagctgatg gagatgcttc ggctactgcg
      241 gtcattcggt gctccgcacg gtgctgccgg acggtgccgg tcgctgcatc ggcgcttcca
      301 caccgctggc ccgcgaacat ttccgttttg cggtcgtgga acgtctatgg actgaggcct
      361 gctcgcggga gcggcccagc atcggggaat gacatgacgc gacgaccggc acgaggtttc
      421 ctggcaggcg cttttctggt gctgttggtg tgttgcggtg cgggctcccc cgcgctggcc
      481 ggggagccgg aggtgtcgtt cgatatcccc gcgcagccac tggcttcggc gcttttgcag
      541 ttttcggacc aggcgggcgc ccagatcgca agcaattcgg tggacgtgtc gaacagctac
      601 agccatggag tccaaggcca gatgccggtc gaagcggccc tgaagcgact gctcgagggc
      661 acggggttct ccttcgtggc gttgccagaa aacacctttg ccgtgcgtcg tggatcgggt
      721 agggccgccg gatccgatcc gcctccctcc gcaacgcacg cattcgacat cccgggcggg
      781 cccttgagcg attcgctgga tcggttcgcc gcccaaagcg gattgcgcgt ggagtacgac
      841 cacgaactcg tccgcggcca ttcggccagg ccgtaccaga acaccgcgcc gtcgctcgtc
      901 gtactcgaag acctgctggc cgggtctggg ctgacctatc gcgtctcggc gccggggacg
      961 gtgatcatcg ctcgccccga gaccacggcc gccgcaccga ccagtgccgc cttgccagct
     1021 cccggcgcgc agggcaccca gcttgcaacc atcacggtga cgggatctcg catcaagcgg
     1081 ccgggctacg acacgctgga ggcgaccagc gtgatcgatt cggcgcaaat cgaaaatcgc
     1141 ggttacacca acgcgctgca ggcgctgcag gcaacccccg gtttcggggc gccttcctcg
     1201 agccccatca gcgcggccca agggcgcatg aacatcggtc agagctttgc gaactttttc
     1261 gggctcggat cgcagcgcac gctgacgctg gtgaacgggc agcgcttcgt gtcatccaat
     1321 ccggtcggta ccaacagcgc caatgcttcg ccgggctcgc aggtcgacct caacctgatc
     1381 cccatcgatc tgatcgaccg gatcgaaacg gtcgcgatcg gcggcgcgcc cgtgtatggc
     1441 tcggatgcga ttgcgggcac ggtcaacatc atcctcaagg acaacttcga gggtgtggct
     1501 gccaccgccc agtatggcat cagcgaaaaa ggcgacgcac agaacaagat ggccagcgtg
     1561 ctgatggggg gcaactttgc gagcaaccgc ggcaacgcag tgatttcggc cgaatacgtg
     1621 caccaggatc ccctggtctt ctccgaccgc tacggcttgt cttacgcgct cccgaatccg
     1681 gcatacaccg gtccgggcga aggcatcccg gcagtgctgt tttatcccga cgtccacttc
     1741 gacttcatga ccgagggcgg catgccctat gacgggtccc tgctcgacat accgggtctc
     1801 cactatcccg gcttgtaccc caacgggaac tacattttcg attccagcgg gcaaccgatg
     1861 cggtttgctc ccgatggtga gttggtgccg ctcgacttcg gaacggtcgt caacagcgcg
     1921 agcctcggcg gcggtatttc cgttcccctg tactcgaccg gcggggacgg cgtcaacgcc
     1981 gccgaccatt tcggcctgtt ggcgggatcc acgcgcaaga tcttcaacgg gatcgcgcat
     2041 ttcgacctta ccgacaacgt gcgcgccttc atcaataccg aatacgcgca taccagctcg
     2101 gtgctgccga gcgacgtgac ctccctgatc gcgccgggac tgttcccaag cccgtccctg
     2161 acgttctcgg tggacaaccc gttcctgtcg gaccaggcgc gcgacatcat cgaggccaac
     2221 gggctgacga gcttcaacct tgcgcgcaac ctcaacgacc tggttgaccg gaatcccgcc
     2281 acgctcgacc tcaacgtgtc ccgggtggtg ttgggcctga agggcaactt cgatgccttc
     2341 ggcaaccaat ggtcatggga cgtcagctac aacaacggca ccagccgctc ggtgtctacc
     2401 gacacttaca tcaaccccga ccgactcgtg ctggctgccg atgcagtgcg cgacgcatcc
     2461 ggcgaaatcg tgtgcgcttc cggtggcgac tgcgtgccga tcgacttgtt tggcgagaat
     2521 gcattcagta gtgccgcggc tgcctacgtc tccgacaggg tcgcgaccac ggcccgcaat
     2581 tcgatgaacg attggcaggc caacatcagt ggcggcctgc cgttcgaaat cgccagagag
     2641 gagcccgtga agatcagctt cggtttcgag caccgggcgg agagtggaag cacccagccg
     2701 gatgccggag ccaagctggg cgacaccttg gtcggtgtac cggggttcac cgccatctct
     2761 ggcagctact cgatcaatgc gttctacggc gaaacgctga ttccgttggt atcggatgcg
     2821 cagcgggtgt cttgggtgaa agcagccagc gttgatctcg cggcccggca cgtcgacaac
     2881 tcgatcaatg gcggtgcctt gacctggtcg gccggcggcc gcttccagcc gaggttcccg
     2941 ggacttggcg acggactggt gtttcgcggc gtattcatgc acgcgatccg ctcgccttcc
     3001 atcaccgagc tgttcctgcc gagctcggga tcgttgggcg gaatagtcga tccctgcgat
     3061 gcaggcaact acaccagcgg accgaatccg gcggcgcgga ctgccaactg cgaagccgcg
     3121 ctcgccgcgg ccggcgcccc acagcccgga gagttccatt cgacgacccg ttccatttcc
     3181 gtagcggggc tccgaaccgg caaccccaat ctcaagaacg aaacggcgga ttcctggtcg
     3241 gttggcatgg tttaccagcc ggtcgagttt ccccggttcc ggatgtcgct cgactggaac
     3301 cacatcaagc tgaaggacgg catcgtctcg ctcggcatcg atgacatcct cgcgtcctgc
     3361 tatgacagct ccaactatcc cgacaatgat gcttgcgccc tcttcagccg cctgaccgca
     3421 gccgaagcga gtaccccacg ggtccccggc gacgtggcac ccggctacaa gcagggcttc
     3481 gtgaattcct cgggcgccga cctgtcaggc gcgattcttg cggcggagta cgtccagccc
     3541 cttccgcacg atgcaggggc gctcaatttc tccggcagcg tgttctacag gaaccactac
     3601 aacgtcgtcg acttcgaagg cggatccgaa acccacgcgg ccgggaccat tgcctatccg
     3661 aaagtccagg ccaccctcag tgccggttac agctggcgtc ggttcgaaac ggtgtggcaa
     3721 gcgcgctgga cgagcggggt cgtcatcgac aacaccgcga ccatcgaaga ctaccccgcc
     3781 tatttcattt ccccttacac gctcgtaagt gccacgttcg gatacaagtt cacgccgggc
     3841 gtgagcctgc ggctgatcgt gaacaacgtc ttcgacaaga agctgccgga cgttgcattg
     3901 gaacagcgcg ccttctcgtt gtatgacccg atcggg
//
LOCUS       DASSNH010000014         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1626755, whole genome shotgun sequence.
ACCESSION   DASSNH010000014 DASSNH010000000
VERSION     DASSNH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626755"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFK00_00370"
     CDS             <1..832
                     /locus_tag="VFK00_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET7591443.1"
                     /translation="RMGYGAMQLAGPGVFGPPKNHNAALAVLREAVEQGINHIDTSDF
                     YGPHVTNQVIREALHPYPDGLHIVTKIGARRGEDGSWNPAFSAEALAGAVRDNLRNLA
                     VDVLDVVNLRAMFDVHGPAEGSIEAPLTALAELQQQGLVRHIGLSNVTPTQVAEGRSI
                     ADIVCVQNHYNLAHRDDDALIDELAAAGIAYVPFFPLGGFSPLQSDTLQSVATRLCAT
                     PMAVSLAWLLERSPNILLIPGTSSLAHLRENIMAAELQLPADAVAELDAIAAQEKVHA
                     "
     gene            complement(1098..>2378)
                     /locus_tag="VFK00_00375"
     CDS             complement(1098..>2378)
                     /locus_tag="VFK00_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HET7591444.1"
                     /translation="QQVQYANFSGWDVYRSQLQLLTWLMPKVGSDIAQSLYNQAQQNH
                     GEWDRWTHNSGGTHVMAGDPSVPAVAGIYAFGGRDFDLQGAYASLKRAATVPTAHDLS
                     DEGCNVECVGQRPSLDQWLKLHYIAAKSNAWGGAGETLEDATADFALSQLAAPAGDRA
                     GERLFLTRAGYWRNLFNPSATPHGGYIQDRNADGTWPAFKPDTGDGFVEGSAAQYLWM
                     VPFDVHGLFGMLGGRAKATARLDDFFHDAHGNWALTKAGPLHAELDNEPSIGTPWLYD
                     YAGQPWKTQKAVRIVLDTLWKNAPDGIPGNDDLGEMSSWYVWAALGLYPAIPGRAELL
                     LGSPLFPKAVVHRAGGDVTIEAPDADRENLYVRALTLDGRPWRKPWLPASFAQHGGTL
                     RFALGAQPDRHWGSDPADAPPSFPPPKQQQGTEQ"
BASE COUNT          370 a          782 c          817 g          409 t
ORIGIN      
        1 tcgcatgggc tacggcgcca tgcaactcgc cggtcccggc gtcttcgggc cgccgaagaa
       61 ccacaacgca gccctggcgg tgctgcgcga ggcggttgag caaggcatca accatatcga
      121 caccagcgac ttctatggtc cgcacgtcac caaccaggtc atcagggagg ccctgcatcc
      181 ctatccggac gggctgcaca tcgtcaccaa gatcggcgcg cggcgcggcg aggacgggtc
      241 atggaacccg gcgttttccg ccgaggcgtt ggcgggcgcg gtgcgcgaca acctccgcaa
      301 tctcgcggtg gatgtcctcg acgtcgtcaa cctgcgcgcc atgttcgacg tccatgggcc
      361 tgccgaaggt tcgatcgagg cgccgctgac ggcgctcgcc gagctgcagc agcagggact
      421 cgtccgccat atcggcctca gcaacgtgac gccgacacaa gttgccgaag gacgatccat
      481 cgcggacatc gtgtgcgtgc agaaccacta caacctggcg caccgggatg acgacgccct
      541 gatcgatgaa ctcgctgcgg ccggcatcgc ctacgtgccg tttttcccac tcggcggctt
      601 ctcgccattg caatccgaca cgctgcaatc ggtggccacg cggctgtgtg ccacgccgat
      661 ggctgtttcg ctcgcctggc tgctggagcg ctccccgaac atcctcctca ttccgggtac
      721 ctcgtccctc gcgcatctgc gcgagaacat catggcggcg gaattgcagc ttccggcgga
      781 cgcggtagcg gagttggatg cgattgcagc gcaggagaaa gtccacgctt gatcccgcga
      841 tacgcggctt tggcccgcgc cgccatcgag acgctgcccg ctgcggcggc tcgtcccttt
      901 cgaggggcga aggggcgcgc ttttgagaag caaggttcgg gcgccggtgg gcgctcgagg
      961 caggacgccg cggaccggct tgttccgcga ggacccgggc gcccgagctt cgcaccgcgg
     1021 cgtggggacg gcgctcgcgg gcgcgggcaa actacgtcgg cgccagcgcg agctgtacga
     1081 gcagcgtcat cggatcttca ttgctcggtt ccttgttgtt gcttcggcgg tgggaatgaa
     1141 ggcggcgcgt cggcgggatc gctgccccag tgccgatccg gttgcgcacc cagtgcgaaa
     1201 cgcagcgtcc cgccgtgctg cgcgaacgac gcgggcagcc aaggtttgcg ccacgggcgg
     1261 ccgtcgaggg tcagcgcgcg cacgtacagg ttttcgcgat ccgcgtccgg tgcctcgatc
     1321 gtgacatcgc cgccggcgcg atggacgacg gccttcggga acagcgggct gccgagcagc
     1381 aactccgcgc ggccgggaat cgccgggtac agcccgagcg ccgcccacac gtaccaggac
     1441 gacatttcgc cgaggtcgtc gttgccgggg atgccgtccg gcgcgttctt ccacagcgtg
     1501 tcgaggacga tgcgaaccgc cttctgcgtt ttccacggtt gcccggcgta gtcgtacaac
     1561 cagggggtac cgatcgaggg ctcgttgtcg agttccgcat gcagcggccc ggccttggtc
     1621 agcgcccaat tgccgtgcgc atcgtggaag aaatcatcga ggcgcgcggt ggccttggca
     1681 cgcccgccca gcattccgaa caggccgtgg acgtcgaacg gcaccatcca gagatattgg
     1741 gccgcgctgc cttcgacgaa gccatcgcca gtgtccggct tgaacgccgg ccaggtgcca
     1801 tcggcgttcc gatcctggat gtagccgccg tgtggcgtgg cgctggggtt gaacaggttg
     1861 cgccagtagc cggcgcgggt caggaacagg cgttcgccgg cgcggtcgcc cgcgggggcg
     1921 gcgagttgcg acagcgcgaa gtcggcggtg gcgtcctcca gcgtttcgcc cgcgccgccc
     1981 caggcgtttg atttggcggc gatgtagtgc agcttcagcc attggtcgag cgacgggcgt
     2041 tggccgacgc attccacgtt gcagccttcg tcgctgagat cgtgggcggt cggcacggta
     2101 gccgcgcgct tgagcgatgc gtaggcgcct tgcaaatcga aatcgcggcc accgaatgcg
     2161 taaattccag cgaccgcagg gacggatggg tcgcccgcca tcacgtgggt gccaccggaa
     2221 ttgtgggtcc agcgatccca ttcgccgtgg ttctgctgcg cctggttgta cagcgattgc
     2281 gcgatatcgc tgccgacctt cggcatcagc caggtcagga gctgcagttg cgagcggtac
     2341 acgtcccagc cggagaagtt cgcgtactgc acctgctg
//
LOCUS       DASSNH010000015         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1859146, whole genome shotgun sequence.
ACCESSION   DASSNH010000015 DASSNH010000000
VERSION     DASSNH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859146"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..1775)
                     /locus_tag="VFK00_00380"
     CDS             complement(300..1775)
                     /locus_tag="VFK00_00380"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7591445.1"
                     /translation="MLEFSLGSAAADTADTPCVVAGLFEDGPSSAAAAIDEASGGAIA
                     RLRESGDFNGKSGTTLVLHGLANVASPRVLLVGLGARDKFDARAFERACGAAGKALKR
                     MPVRQASTWLPELDVAGRDADWRVRTCALAIDHACYRYTATIKPESDAPKLAKLELVA
                     GAGADRGLEQARAIARGVSFARELGCLPPNLCTPSHIAAQAKTIADAHEAVTLEVLER
                     ADMEKLGMGALLAVASGSATPPKLIVLQYRGGEEGAKPCALVGKGVTFDTGGMNLKPT
                     GSMEEMKYDMCGAAGVLGAFLSAVELKLPLNLVCVVPSVENMPGGGGYRPSDVLHTHA
                     GITVEVLNTDAEGRLILCDALAYTCKRFQPQALVDAATLTGACVIALGAHASGLFSAD
                     EELASALLAAGEASLDRAWRLPLWDDYRNQLESAFADIANVGGKSAGAVTAALFLSRF
                     TEGTPWAHLDVAGTAWVPGRKGYATGRPVPLLAEWLIGRSA"
BASE COUNT          306 a          754 c          692 g          323 t
ORIGIN      
        1 gaacgcggcg tgcatcaccg cgtgcggcca ggcatagctg tccgccacga cttcgagcac
       61 ctggatcagc gcgaacgcgg cggcgacgta cgcaatcgcc cactgcacca gcttgcgttg
      121 cttcaagcgt tgcagccaat cattcacggc cagacccttc atggccagcc gccccggctg
      181 catgcgtcgc gatggcgcgc accggaccgg cgggaatcgg gtcgattcta ccgccgccgc
      241 cggcaggcgg tcacttggat cccgtcgcct cgtcgagcgg tggcgcagcg gatcgccggc
      301 tacgcactac gcccgatcag ccactccgcg agcagcggca ccggccgtcc ggtcgcgtag
      361 cccttgcgtc ccggaaccca tgcggtgccg gcgacgtcga ggtgcgccca cggcgttccc
      421 tcggtgaaac gcgacaggaa caaggcggcg gtgaccgcgc cggcgctctt gccaccgaca
      481 ttggcgatgt cggcgaacgc cgattcgagc tggttgcggt agtcgtccca gaggggcaac
      541 cgccaagcgc ggtcgagcga tgcctcgccg gccgcgagca gcgcgctggc cagttcttcg
      601 tcggcgctga acaggcccga ggcgtgcgcg ccgagcgcga tcacgcaggc gccggtcaag
      661 gtcgcggcgt cgaccagcgc ctgcggctgg aagcgcttgc aggtgtaggc cagtgcgtcg
      721 cacaggatca gccggccctc ggcatcggtg ttcagcacct cgaccgtgat cccggcgtgg
      781 gtgtgcagca cgtcgctcgg gcggtagccg ccgccgcccg gcatgttctc gaccgaaggc
      841 accacgcaca cgaggttcag tggcaacttc aactcgaccg cgctgaggaa cgcgccgagc
      901 acgccggcgg cgccgcacat gtcgtacttc atttcctcca tcgatccggt gggtttgagg
      961 ttcatgccgc cggtgtcgaa ggtgacgccc ttgccgacca gcgcgcaggg tttcgcgcct
     1021 tcctcgccgc cccggtactg gagcacgatc aacttcggcg gcgtcgcgga acccgacgcg
     1081 accgcgagca gtgcacccat cccaagtttt tccatgtccg cccgctcgag cacttccagc
     1141 gtcacggcct cgtgcgcgtc ggcgatggtt ttcgcctgcg cagcgatgtg cgacggcgtg
     1201 cagaggttcg gcggcaggca gccaagctcg cgggcgaacg agacgccgcg ggcgatcgcg
     1261 cgtgcctgtt cgaggccgcg atccgcaccg gccccggcga cgagttccag cttcgccagc
     1321 ttcggcgcgt cgctttccgg cttgatcgtc gcggtatagc ggtagcaggc gtggtcgatc
     1381 gccagcgcgc aggtccggac ccgccaatcg gcgtcgcgcc cggcgacgtc cagttccgga
     1441 agccacgtgc tcgcctgcct gaccggcatc cgcttcaagg ccttgcccgc cgcgccacag
     1501 gcgcgctcga acgcgcgcgc atcgaacttg tcgcgcgccc ccaaccccac caggaggaca
     1561 cgcggcgatg cgacgttcgc cagtccgtgc aggaccagcg tcgtgccgga cttgccgttg
     1621 aaatcgcccg attcgcgcag gcgcgcgatg gccccgccgc tcgcttcgtc gatggccgcg
     1681 gcggcggacg acgggccgtc ctcgaacagg cccgcgacga cgcacggcgt gtccgcggtg
     1741 tcggcggccg ccgaacccag gctgaattca agcatgcgta tggctccctt ggcggtgggt
     1801 cgtgcgcagg cgcgccaata cactcggccc tcgcaaagcg cttgagtcta gggaacattc
     1861 ggataacctg ccagtttccg cttcgctcgc gcgcggcgca agcgcggacg gcagcgccgg
     1921 atctcccggc gacaccgcac accgccttga cccccggggt gatccccgcg acaggttcga
     1981 accacgcaat gccgcgtttg tccatcctcg accgctacct cctgcgcgaa ttcctgttcg
     2041 gcaccctggc ggccgtcgtg gtgctgctgg tgatc
//
LOCUS       DASSNH010000016         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1624532, whole genome shotgun sequence.
ACCESSION   DASSNH010000016 DASSNH010000000
VERSION     DASSNH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624532"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VFK00_00385"
     CDS             <1..118
                     /locus_tag="VFK00_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HET7591446.1"
                     /translation="SSDQIKVVSYGEERPVCREHNEGCWHQNRRVNIVYTTE"
     gene            123..914
                     /gene="ybgF"
                     /locus_tag="VFK00_00390"
     CDS             123..914
                     /gene="ybgF"
                     /locus_tag="VFK00_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET7591447.1"
                     /translation="MTNAVRMRRKPRKPAMVMAASAAMIVAASCGTAWAQEPTPPPAP
                     PSNSLVLVNQIQSLQAQMRQMQGEIEELQHALQQLQQANKDQYVDLDSRIGKLERAQP
                     ATAGSAAPTMASSTNAAPAASAAEPPPSADEATVQTDYQAAFKSLRDGNYVDSARRFR
                     AFIDQYPQSPLVSNAYYWLGSSYQVTQNYKPALAAFQALLRQYPDSSKAPEAQLRVAD
                     CQIALKDYGAARRTLQAAIAANPGTQIEQRARDKLQDLPPPSGAQ"
     gene            922..1629
                     /gene="queC"
                     /locus_tag="VFK00_00395"
     CDS             922..1629
                     /gene="queC"
                     /locus_tag="VFK00_00395"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET7591448.1"
                     /translation="MTIGRAEPRRAVVLVSGGMDSAVTLALAREAGFDCHALSVAYGQ
                     RHASELVAAARVAHMLGAVAHKTVSVGLRSIGGSALTSDTEVPEHSDAHGEHDIPATY
                     VPARNTIMLAIALGWAETLDSSDLFCGVNAVDYSGYPDCRPAFVEAFERLANLATRAG
                     VEGARLQVHAPLIDMTKADIVREGVRLGVDFSATVSCYRADPEGRACGHCDACVLRAR
                     GFADAGTADPTRYVAAA"
     gene            1684..1758
                     /locus_tag="VFK00_00400"
     tRNA            1684..1758
                     /locus_tag="VFK00_00400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          362 a          701 c          666 g          325 t
ORIGIN      
        1 ttcgtccgac cagatcaagg tcgtgagcta cggcgaagag cgcccggtgt gccgcgagca
       61 caacgagggc tgctggcacc agaaccgccg cgtcaacatc gtctacacca ccgagtgacc
      121 gtgtgacgaa cgctgtacgc atgcggcgca agccgcgcaa gcctgcaatg gtcatggccg
      181 cttcggcggc catgatcgtt gctgcgtcct gtggaaccgc atgggcacag gagccgaccc
      241 ctccgccggc cccgccatcg aacagcctgg tgctggtgaa ccagatccag agcctgcagg
      301 cgcagatgcg gcagatgcag ggcgagatcg aggagctcca gcacgcgctg caacaactgc
      361 agcaggcgaa caaggaccag tacgtcgacc tggattcccg gatcgggaag ctggagcgtg
      421 cgcagcccgc cacggccggc agcgcggcgc cgacgatggc atcctccacg aatgcagcgc
      481 cggcagcgag cgcggccgaa ccgccgccgt ccgccgacga agccaccgtc cagaccgatt
      541 accaggcggc gttcaagtca ctgcgcgatg gcaattatgt cgactcggcg cgccgcttcc
      601 gcgccttcat cgaccagtat ccgcagagcc cgctggtttc gaacgcgtat tactggctcg
      661 gcagctcgta ccaggtcacc cagaactaca agccggcact ggccgcgttc caggcgttgc
      721 tgcggcagta cccggacagc tccaaggcac cggaagcgca gctgcgggtc gccgattgcc
      781 agattgcgct caaggactac ggcgccgcgc ggcgtacctt gcaggcggcg atcgccgcga
      841 accccggcac ccagatcgag cagcgcgcgc gcgacaagct gcaggacctg ccgccgccat
      901 ccggcgcgca atgacgcaac gatgacgatc ggccgagcgg aaccgcgacg agcggtcgtg
      961 ctggtttccg gaggcatgga ttcggcggtc acgctggcgc tggcgaggga ggcggggttc
     1021 gattgccacg cgctgtccgt tgcctacggc cagcggcatg cctcggagct cgtggcggct
     1081 gcgcgcgtcg cgcacatgct cggcgccgtc gcgcacaaga cggtgtcggt gggccttcgc
     1141 agcatcggcg gttccgcgct gaccagcgac accgaggtcc cggaacattc cgatgcccac
     1201 ggcgaacacg acatcccggc gacctatgtt cccgcccgca acaccatcat gctggcgatc
     1261 gccctcggct gggccgaaac cctcgattcg agcgacctct tctgcggcgt gaatgccgtc
     1321 gattactcgg gctatccgga ttgccggcct gccttcgtgg aagcattcga gcggctcgcc
     1381 aacctggcga cccgcgcggg cgtggaaggc gccaggctgc aggtgcacgc gcccctgatc
     1441 gacatgacca aggcggacat cgtgcgcgag ggcgtgcgac tcggcgtcga cttctccgcc
     1501 acggtctcct gctaccgcgc ggatccggaa ggccgcgcct gcggccattg cgatgcctgc
     1561 gtgctgcgtg cccgcggctt cgccgacgcg ggcactgccg accccacgcg ctacgtcgcc
     1621 gcggcctgac ccgttacaat gcgcggcttg atgtcgcacg cggtcccgcg tcggcgtctc
     1681 acggggccgt tagctcagcg gtagagcagt ggacttttaa tccattggtc gaaggttcga
     1741 tcccttcacg gcccaccata taggccatgc tgttgcagca aggcaccgag ccccgcgagt
     1801 agcgtgcagg ttcacagttg ccatctggtt gccgattgcg gtggttcgat ctgcagcaac
     1861 gacatcttcg atccggcccg atcgacacag gggcatacgc gcgacgcgtg aacgcaagct
     1921 cgtcattttt gctttgttaa tgcccggttg ctgctggcgc attagcatgg acgctcgtca
     1981 aggaggaacc ccaatggacg ttacgcaagg caatgcaatc cttcacagtg gcgcggaagc
     2041 gctccagaac gccg
//
LOCUS       DASSNH010000017         9200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1858486, whole genome shotgun sequence.
ACCESSION   DASSNH010000017 DASSNH010000000
VERSION     DASSNH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858486"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFK00_00405"
     CDS             complement(<1..1040)
                     /locus_tag="VFK00_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7591449.1"
                     /translation="MCLARVIVAVRGGGIALLLMVIPQFAAAPPAYAASPEPAAASSS
                     REAATRAQLTAVRAEIAKLAEEQHAAATQRDAINAKLAEQAAQLDDAAKALRETNAAI
                     AARTAALADLQIRRVQLEDKLAGQREALADLLRAVYTLDRDAELPLLLGDNDLARIDR
                     ALAYSRYFQQDRVGRIRTLLGEVAQLDGIRSSIEAEAQALQQQRAEHATRNAALEQQR
                     DAQKKLLAEADTRLAQQKDKLASLKHDAEALARLLKQLQNVFADIPAQLGKNTPFAQL
                     RGKLAWPVGGTPRKGTGLLANGIVLAAKPGADVHAVAYGRVAWADFMRGYGMLVIIDH
                     GDGWMSLYGGNES"
     gene            1106..1891
                     /locus_tag="VFK00_00410"
     CDS             1106..1891
                     /locus_tag="VFK00_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET7591450.1"
                     /translation="MRYAARMCVLAFAWNVHPQWRLLLVGNRDELHARPSAPLARWPD
                     APGVIAGRDLEAGGTWMGVGEPGRAAVVTNVRDPRLPHDGLSRGWLVTDFLRGADGAA
                     GHAEELTTDAAHYRPFNLLLFDQQEGRFVSNAPNVRGHAFDAGLHGLSNGGLDAPWPK
                     LARAIAALRDWLDAGKDSFADLLDAFMDDTIAPDTALPDTGVGVDMERQLSPVFVRGG
                     RYGTRATTLIALGRNGGGCIVERRFGPAGVPQGETTLRFGGAA"
     gene            complement(2121..3008)
                     /locus_tag="VFK00_00415"
     CDS             complement(2121..3008)
                     /locus_tag="VFK00_00415"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HET7591451.1"
                     /translation="MNQGRSARGWIIGSIVVIIIAAVAAWWYFTRVPGVDFSRHGPAP
                     SAPAPGSTAPAPVEHPISQAQTVPAPATTSPLPPLDASDDAFAKALSEIPGAEGLAQL
                     LLARGLVPHVVATVDALPRHEIGASILPLKTPTGSFAVDTSGEQPAIAVENYKRYDAY
                     MKIVQDVDPRTLVTWYVHWYPLFQKAYEQLGYPRGYFNDRLVQAIDNMLAAPNVQRAP
                     QLVKTQDGHYVFADPTWEALSVGQKLMIRIGPDHERELKSRLRSIRALLLGKAPVLHG
                     QAAPAASAASAPAAATTAQ"
     gene            complement(3357..3863)
                     /locus_tag="VFK00_00420"
     CDS             complement(3357..3863)
                     /locus_tag="VFK00_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HET7591452.1"
                     /translation="MTISMYQASVPVFARALTNLSNVLKKGEAHAKDKGVDPDTLLQQ
                     RLIFDMLPLIKQIQIACDTAARGAARLAGVEPQSFPDNETTLAQASDRIARTIDYIKS
                     FKPEQIDGSEGRKVVMKTRHGDVDFVGLAYLTEYALPNLFFHCATAYDILRVAGTQIG
                     KKDYLGDR"
     gene            complement(3936..4136)
                     /locus_tag="VFK00_00425"
     CDS             complement(3936..4136)
                     /locus_tag="VFK00_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591453.1"
                     /translation="MMTFLLWLLLLIFCWPLALLALILYPLLWLILLPFRLVGVAVDG
                     VFDLLRGLLSLPGRILRGPERM"
     gene            complement(4200..5471)
                     /locus_tag="VFK00_00430"
     CDS             complement(4200..5471)
                     /locus_tag="VFK00_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HET7591454.1"
                     /translation="MSERGQDHPRPRAAVIFIFVTVTIDMLAFGIIIPVLPHLIVELI
                     GGSIAKAAVWTSGFGTLFMLMQFVCSPIQGTLSDRFGRRTVILISSFGLGVDFIVMAL
                     APVLWLLFVGRAVSGMCAASFSTANAYIADITPREKRAAAFGMLGGAFAVGFIVGPAL
                     GGFLGHLWIRLPFWVAAVLCLINFCYGFFVLPESLPRERRAPRFDWRHANPLGSLLLL
                     KRHPQVLGLASVVFLTALAQFAINSTYVLYTDYRFGWGPQTVGYTLTLVGVLTGVVQA
                     LLVRRLMPKLGERRMILAGLALWVAGMACFGFAPTAWIFLVGIPILCLGGLAGPPTQS
                     LITHQVDPAEQGRLQGALSSLQSLAGIVGPALFANLFALFISEHAPLGGHLPGIAFVL
                     AAALTLLALILATRATREMPGHASAVEPKTT"
     gene            complement(5603..6235)
                     /gene="folE"
                     /locus_tag="VFK00_00435"
     CDS             complement(5603..6235)
                     /gene="folE"
                     /locus_tag="VFK00_00435"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7591455.1"
                     /translation="MSETEKPSLCAARKPTSPEVTREQAMDAVRTLLRWSGDEPTREG
                     LRDTPKRVVDAYSEWFSGYSEDPEDFLRRTFKEVAGYDEMIVLRDIEFESHCEHHLAP
                     IIGRVHVGYLPDGKVVGISKLARVVDAFAHRMQVQEKLTAQIATCIQTVLNPRGVGVV
                     VDATHQCMTTRGVHKRGVSMITSQMLGSFREDARTRSEFLNFIGIDSGHR"
     gene            complement(6306..7760)
                     /gene="rmuC"
                     /locus_tag="VFK00_00440"
     CDS             complement(6306..7760)
                     /gene="rmuC"
                     /locus_tag="VFK00_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7591456.1"
                     /translation="MDTTLLYVLVALAALAVLLLAMLLLRPRGDAGVRERLDALRGDN
                     ERVERTVRDEQGKGRAELAQSFEQFRGHVQAQLDTVSTHQRQRIDEFSTRLAQLIERT
                     DRGGESLRNQLIDDARKAREEGQASLQRFSEQLQASLKALTGDNEKRLAEVRGTLDAQ
                     LKALQTDNAAQLDKMREVVDEKLQKTLEARLSASFKQISDQLGAVQRGLGEMQTLATG
                     VGDLKRVLSNVKTRGAFGEVRLGALLEQILTADQYASNVPVVPHSSERVEYAIRLPGQ
                     ASDAPVWLPIDCKFPVEDYQRLLDAQEAADVDAANAAAAALQRCVEIEAKRIRDKYVS
                     PPHTTDFALLFLPTEGLYAEVIRRPGLFEKLQRDHHITVAGPTTLAAILNALQMGFRT
                     LAIEQRSSEVWQILGAVKTEFGKFGGMLDSAIRQVDTVRNTLKKATGKTTTIGRKLRG
                     VEALSAPDSQALLGSETADEPDEADEDEMPAGPA"
     gene            complement(7907..8350)
                     /locus_tag="VFK00_00445"
     CDS             complement(7907..8350)
                     /locus_tag="VFK00_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HET7591457.1"
                     /translation="MAIWKQQADLATVNAWSRGTLMQAIGIRITEIGDDFLAGTMPVD
                     ERTRQPYGILHGGASVALAETLGSTAAVLCCEDGTAAVGLDINANHLRAVREGTVTGI
                     ARPIHIGRSTHVWEIRIANEAGEPTCIARLTMAIVPKRAVAPKPS"
     gene            complement(8414..>9200)
                     /locus_tag="VFK00_00450"
     CDS             complement(8414..>9200)
                     /locus_tag="VFK00_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HET7591458.1"
                     /translation="EEAMQLLAPGAEAGKLPPAGWYEYALAALATHQPDKARAALEPL
                     RKSDMLGAQGQADLVADVLGQSLRLAPDAAALQDLWKRAIRPQRQVPAVLEAYASRAY
                     ELGVSHDALVAIEEALKREWNPALVAAYGNATPPAEAGVRLHNASRWLDAHPGDETLL
                     RTLGVLSMRAADPRAAREYLVRSLSSKPTAAGWAALGEVQSATGETADATRCFANAWH
                     CSQGRDITPGPHEGGIDTAPVTPEERDEHGVPRLPGEPPGPVR"
BASE COUNT         1409 a         3038 c         3184 g         1569 t
ORIGIN      
        1 gactcgttgc cgccgtacag gctcatccag ccgtcaccat ggtcgatgat caccagcatc
       61 ccgtagccgc gcatgaagtc ggcccaggcc acccggccat aggcgactgc gtgcacatcc
      121 gcgccgggct tggcggccag cacgatgccg ttcgccagca gcccggtgcc cttgcgcggc
      181 gtgcccccca ccggccacgc gagcttgccc cgcagttgcg cgaatggggt gttcttgccg
      241 agctgcgcgg ggatgtcggc gaagacgttc tggagttgct tgagcaagcg ggcgagcgcc
      301 tccgcatcgt gcttcaggga ggcgagtttg tccttctgct gcgccaatcg tgtgtccgct
      361 tcggcgagca gcttcttttg cgcgtcccgt tgctgttcga gcgcggcatt cctggtggcg
      421 tgctcggcgc gctgctgttg cagggcctgg gcttccgctt cgatcgagct gcggatcccg
      481 tcgagctgcg cgacttcgcc gagcagcgtc cggatacggc cgacgcgatc ctgctggaaa
      541 tatcgcgagt aggcgagcgc gcgatcgatc cgcgcgaggt cgttgtcgcc gagcagcagc
      601 gggagctcgg cgtcccggtc cagcgtgtag acggcacgca gcaagtccgc cagcgcctcg
      661 cgctggccgg ccagcttgtc ttcgagctgc acgcgtcgga tttgcagatc cgcgagcgcg
      721 gcggtcctgg cggcaatcgc ggcattggtc tcgcgcaggg ccttcgcggc gtcatccagc
      781 tgggcggcct gttcggcgag cttcgcattg atcgcgtcgc gttgtgtcgc ggccgcgtgt
      841 tgctcctcgg caagtttcgc gatctccgca cgaacggcgg tgagttgcgc acgggtggcg
      901 gcttcgcggc tggaactcgc ggcggcgggt tccggcgatg cagcgtacgc gggtggtgcg
      961 gccgcgaact gagggatcac catcagcagc agcgcgattc caccgcctcg gacggcgacg
     1021 atgacgcggg caagacacat gggccgcgat tatcgccgcg catgcacgcg cgcgggcaac
     1081 gtatcgacgt ccagtggaat ccacgatgcg ctacgctgcc cggatgtgcg tgcttgcatt
     1141 cgcgtggaac gtccacccgc aatggcggtt gctgctggtg ggcaaccgcg acgaattgca
     1201 cgcgcgtccg tcggcaccgt tggcgcgctg gcccgatgca cccggcgtga tcgccggacg
     1261 cgacctcgaa gccggcggaa cctggatggg cgtgggcgag cccgggcgtg ccgcagtggt
     1321 gacgaatgtg cgcgatccgc ggctgccgca tgacgggctg tcgcgcggct ggctcgtcac
     1381 cgatttcctg cgcggagcgg acggcgctgc gggccacgcg gaggaactca cgacggatgc
     1441 cgcgcactat cgcccgttca acctgctgct gttcgatcag caggaggggc gcttcgtctc
     1501 gaatgcgccg aacgtgcgcg gacacgcgtt cgacgccggg ctgcacgggt tgtcgaacgg
     1561 cggactggac gcgccctggc cgaaactcgc acgcgcgatc gcggcgctcc gcgattggct
     1621 ggacgccggg aaggacagct tcgccgatct gctcgacgcc ttcatggacg acaccatcgc
     1681 gccggacacg gcgctgccgg ataccggcgt cggcgtcgac atggaacggc agttgtcgcc
     1741 ggtgttcgtg cgcggcggac gctacggcac gcgtgcgacg acgctgatcg cgctcggccg
     1801 caacggtggc ggctgcatcg tcgaacgccg cttcggcccg gcgggcgtgc cgcagggcga
     1861 gaccacgcta cgcttcggcg gcgccgcatg aagccctccc gttcaaggga ggcagccgca
     1921 ggccgagcgc gtagcgctgg tgcggatggg ttcacgacgc cggacgagga aaccggtcct
     1981 cttgccgccg acaatgccgg gtctacgacc cagctgtgcg tagggcggaa ccggcgccgc
     2041 cgcttccgcc gctcggccca acgtcgattt tgccgaaacc gcctcgcggg tttctccggt
     2101 ctccctttga acgcgggacg ctattgcgcg gtcgtcgccg ctgccggtgc ggaagcggcg
     2161 gaggccgctg gcgcggcctg cccgtgcagg acgggtgcct tgcccagcag cagggcgcgg
     2221 atgctgcgca accgggattt cagttcgcgc tcgtggtcgg gcccgatgcg gatcatcagc
     2281 ttctggccca ccgacagcgc ctcccaggtc gggtcggcga acacgtagtg gccatcctgc
     2341 gtcttcacga gctgcggcgc tcgctgcacg ttcggcgcgg ccagcatgtt gtcgatcgcc
     2401 tgtaccagcc ggtcgttgaa gtagccgcga ggatatccga gttgctcgta cgccttctgg
     2461 aacagcgggt accagtgcac gtaccaagtg accagcgtgc gcggatcgac atcctggacg
     2521 atcttcatgt aggcgtcgta gcgcttgtag ttttccaccg cgatggccgg ctgctcgccg
     2581 ctggtgtcca ccgcgaagct gccggtcggg gttttcagtg gcaggatgct cgcgccgatc
     2641 tcgtgccgcg gcaacgcgtc gaccgttgcg acgacgtgcg ggacgagccc gcgcgccagc
     2701 agcagttggg ccaggccttc cgcgcccggg atttcggaga gcgccttcgc gaaggcgtcg
     2761 tcgctggcat ccagcggcgg caacggcgag gtggtggccg gcgccggaac ggtctgcgcc
     2821 tggctgatcg gatgctccac cggtgcgggc gcggtgctgc cgggcgccgg tgcggagggc
     2881 gccggtccat ggcgcgaaaa atccacgccc ggcacccgcg tgaaatacca ccaggccgcg
     2941 accgcggcga tgatgatcac cacgatcgat ccgatgatcc agcctctcgc cgacctgcct
     3001 tgattcatgc cttcgccttg ttcagcaaac ctttaggctt cgagtcccca ttgtacgaat
     3061 ggttccgtgc cggcgcagcc gtcccacggc agcgggcgtg caggacgtgc gccgggcatc
     3121 cgggtggttc ttcttggcga tttggagcgc ttggaatcct ggcgtccatg cttcgagttg
     3181 ggcttcgccc ggaccgagtc cgggctcaac ccaacctacg cccgccacat catcgaacgt
     3241 aggttgggtt ggctccggat tcattccgga ggaagcccaa catcccaagg tctgcaaggt
     3301 tgtggtcacc ctgcatgagc ccgggccgcc ccgacctgcg ttcgcccgcg gtccgctcag
     3361 cgatcgccga ggtaatcctt cttgccgatc tgggtgccgg ccacgcgcag gatgtcgtag
     3421 gcggtggcgc agtggaagaa caggttgggc agcgcgtact cggtcaagta ggcgaggccg
     3481 acgaagtcca cgtcgccgtg gcgggtcttc atcacgacct tgcggccttc gctgccatcg
     3541 atctgttcgg gcttgaacga cttgatgtag tcgatggtgc gcgcgatgcg atcggatgcc
     3601 tgcgcgagcg tggtctcgtt gtcggggaac gactgcggct cgacgccggc gagccgggcc
     3661 gcgccgcgcg cggcggtgtc gcaggcgatc tggatctgct tgatgagcgg cagcatgtcg
     3721 aagatcagcc gttgctgcag cagggtgtcg ggatcgacgc ccttgtcctt cgcgtgcgcc
     3781 tcgcccttct tcaggacatt ggacagattg gtaagcgcgc gggcgaacac ggggaccgag
     3841 gcttgataca tggaaatcgt catcttggaa ctccagtggc agttgaacga aatcttttgt
     3901 gcgggtgcgt gggccggccg gcgggtgcca gtgcgtcaca tccgctcggg gccgcgcaga
     3961 atgcgccctg gcaggctgag caatccgcgc agcaggtcga acacgccatc cacggcgacg
     4021 ccgaccagcc ggaacggcag caggatcagc cagagcaacg gataaaggat gagtgccagc
     4081 agcgcgaggg gccagcagaa gatcagcagg agcaaccaca acaggaaagt catcattgca
     4141 gcattccccg aggtccgcgc gagacgttca gatgaggcca tgggctgcgg aatgcaagtt
     4201 caagtcgtct tgggttcgac ggccgatgca tggccaggca tttcgcgcgt ggcgcgcgtc
     4261 gccagtatca atgcgagcag cgtcagtgct gcagccagca caaaggcaat ccccggcaaa
     4321 tgaccgccca gcggcgcatg ctcgctgatg aacagtgcga acaggttggc gaacagcgcg
     4381 ggacccacga tgccggcgag gctttgcagg ctggacaggg cgccctgcag gcggccctgt
     4441 tcggccggat cgacctggtg cgtgatcagc gattgcgtcg gcgggccggc gagaccgccc
     4501 aggcaaagga tcgggatgcc gaccaggaat atccacgccg tcggcgcgaa cccgaagcaa
     4561 gccatgccgg ccacccacag cgccagtccc gccaggatca ttcggcgttc gccgagcttc
     4621 ggcatcaggc ggcgcaccag cagcgcctgc accaccccgg tcagcacgcc gaccagcgtc
     4681 agggtgtagc cgaccgtctg cgggccccaa ccgaaccggt aatcggtgta cagcacgtag
     4741 gtcgagttga tcgcgaattg cgccagtgcc gtcagaaaca ccacgctcgc aagtcccagt
     4801 acctgcggat ggcgtttgag cagtagcagt gaacccagcg gattggcgtg gcgccagtcg
     4861 aacctcgggg cgcggcgttc ccttggcagc gattccggca gcacgaagaa gccgtaacag
     4921 aagttgatca ggcacaggac cgcggccacc cagaacggca gtcgaatcca gaggtgcccc
     4981 aggaagccgc ccagcgccgg ccccacgatg aaacccacgg cgaaggcgcc gcccagcatg
     5041 ccgaaagcgg cggcgcgttt ctcccggggc gtgatgtcgg cgatgtaggc gttggccgtc
     5101 gagaaactgg ccgcgcacat gccggacacg gcgcggccga cgaacagcag ccatagcacg
     5161 ggcgccagcg ccatcacgat gaaatccact ccgagtccga agctcgaaat caggatcacg
     5221 gtgcggcggc cgaaacggtc cgacagcgtg ccctggatcg gcgagcacac gaattgcatc
     5281 agcatgaaaa gggtgccgaa gccgcttgtc cacaccgcgg ccttcgcgat gcttccgccg
     5341 atcagttcga cgatcaggtg cggcagcacc gggataatga tcccgaacgc cagcatgtcg
     5401 atcgtcacgg tgacgaagat gaaaatcacc gccgcgcgcg ggcgcggatg gtcctgtccc
     5461 ctctcgctca tggctgcgtg gacaaaaact caggccgtgt gggtttcggg atgcctctcg
     5521 cccggcaggc gatcagcgcc tgtcctgctc tcccgggcgt gaagcggacg gggggaagaa
     5581 aaattgcgcg cacggaaccc gctcaccgat gcccgctgtc gattccgatg aaattgagga
     5641 actcggaccg tgtacgcgcg tcctcgcgga aactcccgag catctggctg gtgatcatcg
     5701 acacgccgcg cttgtgcacg ccgcgggtgg tcatgcattg gtgggtggcg tccaccacca
     5761 cgccgacgcc gcgcgggttc agcacggtct ggatgcaggt ggcgatctgg gccgtcaact
     5821 tttcctgcac ctgcatgcgg tgcgcgaagg cgtccaccac gcgcgcgagt ttcgaaatcc
     5881 cgaccacctt gccgtccggc aggtagccca cgtgcacccg tccgatgatg ggcgcgagat
     5941 ggtgttcgca gtgcgattcg aattcgatgt cgcgcagcac gatcatctcg tcgtagccgg
     6001 cgacctcctt gaaggtgcgg cgcaggaagt cctcgggatc ctccgaatag ccggagaacc
     6061 attcgctgta ggcgtcgacg acccgcttgg gcgtgtcgcg caggccttcg cgggtcggtt
     6121 cgtcgccgga ccagcgcagc agcgtgcgca ccgcatccat ggcctgctcg cgcgtgactt
     6181 ccggtgaagt cggcttgcgg gcggcgcaca gggaaggctt ttcagtctcg ctcatcggat
     6241 ggcctcgtcg cgcgtcgagc ccgtcagtgt agcgcgcgcc cccggccgat ggccggcggc
     6301 atgggtcaag ccggtcccgc gggcatttcg tcttcgtccg cctcatccgg ttcgtcggcg
     6361 gtttcactgc ccagcaacgc ctgcgaatcc ggtgcgctca gcgcctcgac gccgcgcagc
     6421 ttgcggccga tggtggtggt cttgccggtc gccttcttca gcgtgttgcg gaccgtgtcg
     6481 acctggcgga tcgcggagtc gagcatgccg ccgaacttgc cgaactcggt cttgaccgcg
     6541 ccgaggatct gccacacctc gctgctgcgc tgctcgatcg ccagcgtgcg gaaacccatc
     6601 tgcagcgcat tgaggatggc cgcgagcgtg gtcggccccg cgacggtgat gtggtggtcg
     6661 cgttgcagct tctcgaacag gccgggccgc cggatcacct cggcgtacag gccttcggtc
     6721 ggcaggaaca gcagggcgaa atcagtggtg tgcggcggcg agacgtactt gtcgcggatc
     6781 cttttggctt cgatttccac gcagcgttgc agggccgcgg ccgcggcgtt ggcggcgtcg
     6841 acatcggccg cctcctgtgc atcgagcagg cgctggtaat cctcgaccgg gaacttgcaa
     6901 tcgatcggca gccacaccgg cgcgtcgctc gcctgtcccg gcaggcggat cgcgtattcc
     6961 acccgttcgc tggaatgcgg caccacgggg acattggagg cgtattggtc ggcggtgagt
     7021 atctgttcaa gcaaagcgcc gaggcgcacc tcgccgaacg cgccgcgtgt cttcacgttg
     7081 ctgagcacgc gcttcaggtc gccgacgccg gtcgccagcg tctgcatttc gccgagcccg
     7141 cgctgcaccg cgccgagctg gtcggaaatc tgcttgaacg acgcggacaa gcgcgcttcc
     7201 agcgtcttct gcagcttctc gtccaccacc tcgcgcatct tgtcgagctg ggcggcgttg
     7261 tccgtctgga gtgccttcag ttgtgcgtcg agggtgccgc ggacctcggc gaggcgcttt
     7321 tcgttgtcgc cggtcaatgc cttgagcgat gcctgcaact gttcgctgaa gcgttgcagc
     7381 gaggcttggc cttcctcgcg cgccttgcgg gcatcatcga tcaactggtt gcgcagcgac
     7441 tcgccaccgc ggtcggtacg ttcgatgagt tgcgccagcc gggtggagaa ctcgtcgatg
     7501 cgctggcgct gatgggtgct gacggtgtcg agttgcgcct gcacgtggcc gcggaactgt
     7561 tcgaacgact gcgcgagttc cgcccggccc ttgccctgtt catcgcgcac cgtgcgctcg
     7621 acccgctcgt tgtcgccgcg caacgcatcc agccgttcgc gcacgcccgc gtcgccgcgc
     7681 ggccgcagca gcaacatcgc caacagcagc accgcgagcg ccgcgagcgc gacgagcacg
     7741 taaagcaggg tcgtatccat gcggagattg tagggccggg actgacgcaa agttacgcca
     7801 ttaccgtcgc attgcgcgag acggcgcgct cacaacccgg cgagtgccat gcagggcggg
     7861 caatgttcgc cgcttggcgt gccacgcggc ggcagacgat ctccgcctac gagggcttcg
     7921 gcgccaccgc ccgcttcggc acgatcgcca tcgtgagacg cgcgatgcag gtcggttcgc
     7981 cggcttcgtt cgcgatgcgg atttcccaaa cgtgggtact gcggccgatg tggatcggtc
     8041 gcgcgatgcc ggttaccgtg ccctcgcgca ccgcgcgcag gtggttggcg ttgatgtcga
     8101 gccccaccgc ggcggtaccg tcttcacagc acaacacggc cgcggtcgag ccgagggttt
     8161 ccgcgagcgc caccgacgcg ccgccgtgca ggattccgta cggctgccgg gtgcgctcgt
     8221 ccaccggcat cgtgcccgcg aggaaatcgt cgccgatttc ggtgatgcgg atgccgatcg
     8281 cctgcatcaa agtgccgcgg ctccaggcat tgacggtggc gagatccgct tgctgtttcc
     8341 agatggccat gggagtcgtt cgtccccgat gtcgcaggcg cgcgcgcgtc cgctggaccg
     8401 gccagtcggt gcgctagcgc accggcccgg gtggctcgcc cggcagccgc ggcacgccgt
     8461 gctcgtcgcg ctcctcgggc gtgacgggtg cggtgtcgat gccgccttcg tggggccccg
     8521 gcgtgatgtc gcggccctgt gagcagtgcc acgcattggc gaagcagcgg gtggcgtcgg
     8581 ccgtttcgcc ggtggccgat tgcacttccc cgagcgcggc ccaacctgcg gcggtcggct
     8641 tcgacgacag cgagcgcacc aggtattcgc gcgccgcgcg cggatcggcg gcacgcatcg
     8701 agagcacgcc gagcgtccgc agcagcgttt cgtcgcccgg atgcgcatcg agccagcggc
     8761 tggcgttgtg caggcgcacg ccggcttcgg cgggcggcgt cgcgttgccg taggcggcga
     8821 ccagcgccgg attccattca cgcttcaggg cttcctcgat ggcgaccagc gcgtcgtgcg
     8881 aaacgccgag ttcgtacgcg cggcttgcgt acgcctccag gaccgccgga acctggcgct
     8941 gcggccgtat ggcgcgcttc cacaaatcct gcagggccgc ggcgtcgggc gccagccgca
     9001 acgactggcc cagcacgtcg gccacgagat cggcctggcc ttgcgcgccg agcatgtcag
     9061 acttgcgcag cggttccagc gcggcgcggg ccttgtccgg ctggtgggtg gcgagtgccg
     9121 ccagtgcgta ttcgtaccag ccggccggtg gcagcttgcc ggcttcggcg ccgggcgcca
     9181 gcagctgcat ggcctcctcg
//
LOCUS       DASSNH010000018         8964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_847638, whole genome shotgun sequence.
ACCESSION   DASSNH010000018 DASSNH010000000
VERSION     DASSNH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847638"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFK00_00455"
     CDS             complement(<1..263)
                     /locus_tag="VFK00_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7591459.1"
                     /translation="MDPTSPVRIKGRGAATNPEGRFETMRHYAEDDGWQSALLDESAP
                     RPRTEVNEERARSIISRNDSPDVAFEQSINPYRGCEHGCIYCFA"
     gene            357..749
                     /locus_tag="VFK00_00460"
     CDS             357..749
                     /locus_tag="VFK00_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7591460.1"
                     /translation="MDASLLVIQPTSAEPIYRQIVEQLRRLIAGGQLVPGDLLPSVRE
                     VAGFHAVNPMTVSRAYSLAEGEGLLQRLRGKGMAVAAQRNASHNPAQRLALLEPQLAA
                     LARQARELELPAEQVLRRLDKMMGSKAP"
     gene            746..1645
                     /locus_tag="VFK00_00465"
     CDS             746..1645
                     /locus_tag="VFK00_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7591461.1"
                     /translation="MNAVARNVSAESAVPLQVGGLVHRYDSKLVLDQVSLSLEPGSVL
                     GLIGRNGAGKSTLIRAMLGLLEPDAGSAFVFGEPALKLPDDVKERLGYVPQQPDALAW
                     LTATQMFDYLSRFYPRWDGRFVENTMQRWRIAGDKVLAKLSPGERQRVDLVRALASQP
                     ELLVLDEPAAALDPVARRELLREIALRAGESGTTVLFSTHIVSDLERVASDIAFLHEG
                     KLLLHCPVDDTKERYARLWLPANVAYAAPPSVFGKRRHEDGSVSVIVERDSDGAWPDA
                     ARLPAARIDALGLEDLFVEIAEP"
     gene            1642..3144
                     /locus_tag="VFK00_00470"
     CDS             1642..3144
                     /locus_tag="VFK00_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591462.1"
                     /translation="MNTSVADVPASHKGTSMRFIDILVAPRYATSASTRWASVGFFVL
                     LAGAAVVIGIISHDPKARFAALICYTFGITMVWWLWLSGLVLIARDGRGLGLPRAGRE
                     SAYAACLYAAAMVAAPALLVAASGGSVAAAVLFPALAIAGSLAWTLLPRWLAMWFGFM
                     PAIYIGLHNAFHVPSPLDPRFRNWAWLTLAMLVVVVVLRWRQMARGEDNDDSRWSSAM
                     ILQMRRHAVSCDWWSMDRNWAWRRSRGRKIDVDFRGIGAANPALAIRVALGDWYVPQS
                     WRSRAGSLARVLLPLLLFIPLMVFMNIGRYVSLHTVWKVIGISGGLWIGLFGSAMLAL
                     TVAARVQRRWRGSADLGLLALLPGLGAAAASHVLRATFLGPAIAFVALWLCMVAGIAL
                     AAPGVLATLLATLFVLEMAALMAMAGLRAMAGLRMRFMVKVAIGVVLIVLGDSGFTLA
                     FVSPTQASAALVGAVVLGWLLLIAWTVWRGWLAWRLLQRRPHPFLATPPQ"
     gene            3257..3514
                     /locus_tag="VFK00_00475"
     CDS             3257..3514
                     /locus_tag="VFK00_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591463.1"
                     /translation="MIWGLAIVSLLAFALAFTSHSPGLMGLGVVVGFLCGIAAALVFI
                     DRHLRASSRPEHMTEREISALRDTIRKPEESRRKLPPGPSA"
     gene            3585..5540
                     /gene="acs"
                     /locus_tag="VFK00_00480"
     CDS             3585..5540
                     /gene="acs"
                     /locus_tag="VFK00_00480"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7591464.1"
                     /translation="MNMVHPVPSAFAAAERIKPDEYERRYAESLRDPEAFWAEAAKRL
                     DWIKPPTQIRDVSFDQEDLHIRWYADGVLNPTVSCLDRHLDKRGDKTALLFEGDDPSV
                     SRKISYRELHAEVCRFANALRNLGVVKGDRIAIYMPMVPEACIAMLACARIGAIHSVV
                     FGGFSPDALAGRIADSACKLVITADEGVRANKKIPLKANVDEALTRPGTTSIETVVVV
                     RRTGGAVNMQSPRDRWWDALVDGQPDQCEPEPMNAEDPLFILYTSGSTGKPKGVLHTV
                     GGYMVWVSLTHEAIFDLREDDIYWCTADVGWVTGHSYIVYGPLANGATSLVFEGVPTW
                     PDASRIWQVCDKHQVTILYTAPTAIRALMRAGDEPVKQTSRESLRLLGSVGEPINPEA
                     WEWYFNVVGEGRSPIVDTWWQTETGGIMICPLPGSTTLKPGAAMKPFFGIRPAILDAG
                     GGVIEGEGSGNLVITESWPGQMRTVYGDHQRFIETYFKMYPGVYTTGDGAERDADGDW
                     KITGRVDDVINVSGHRIGTAEVESAMMADLRVAEAAIVGCPHDIKGTTLYAFVTPKEG
                     VAPSDALKKELIAGIRERIGAFAAPEYLQWAPALPKTRSAKTMRRILRKIAEAGDHEV
                     DLSALGDTSTLADPSIVKRLYDERVRH"
     gene            5569..6249
                     /locus_tag="VFK00_00485"
     CDS             5569..6249
                     /locus_tag="VFK00_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET7591465.1"
                     /translation="MHELTFADADPAAVAGQLDDGSIHLWRMPYAQSQGRAPLLQLLG
                     AYLGRAASEVSLVEGPHGKPGLPGSKSQRGETRRLEFNWSHSGGSALVALARGRAVGV
                     DIERLGRRMRTLELARRFFDPAEGDALESLAFSERDSAFIGLWCAKEAVLKAAGEGLS
                     FGLARLVFVPHPRAGWKLDRVDSALGGGNEWRIVGFEPAPGYHGALAWHGPEPRISAF
                     QERMATGG"
     gene            6517..7284
                     /locus_tag="VFK00_00490"
     CDS             6517..7284
                     /locus_tag="VFK00_00490"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET7591466.1"
                     /translation="MTVLSVNLNKIAVLRNARGGAEPSLARAADTCIAAGCGGITVHP
                     RPDRRHITPEDVALLAELTRGRVEFNIEGNPFAPPRPGYPGMLELVRQARPAQATLVP
                     DSDGQLTSDHGFDLVRDAPKLAPLVSELVATGCRVSLFVDAGSAEGFDRARDLGAARV
                     ELYTGPYARAFAEGDATDELSRCMDTARRAGIAGLAVNAGHDLNQQNLGAFRRAIPGL
                     AEVSIGHALIGEALYEGLDRTVRNYVAILSRESGPSY"
     gene            complement(7516..7965)
                     /locus_tag="VFK00_00495"
     CDS             complement(7516..7965)
                     /locus_tag="VFK00_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7591467.1"
                     /translation="MAMSAGDNTGSDIPKAEINVTPLADVMLVLLIIFMITAPLMSHK
                     VKVDLPRANPETKPSVVEVPPIDLAVEPDGTLYWNDNQISDAVLSARLRAAATVQPQP
                     ELNIRADKTTQYKIIWKVMQEAKDAGMVHIGFITQGNAKAVPGSVVH"
     gene            complement(7978..8385)
                     /locus_tag="VFK00_00500"
     CDS             complement(7978..8385)
                     /locus_tag="VFK00_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7591468.1"
                     /translation="MAMSTNSGGGPVAEINITPLIDVMLVLLVIFMITAPLVQHNIKI
                     DLPQPNPNVKPPENPPTPKTLFIRADGAMTWDNLPISSVELQAQFATIGAKTIDMQPQ
                     VHIKATDETQYNYLAQVMAEAKRYNVQKIGFEK"
     gene            complement(8398..>8964)
                     /locus_tag="VFK00_00505"
     CDS             complement(8398..>8964)
                     /locus_tag="VFK00_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7591469.1"
                     /translation="PSAQDAVRVMEKQPRSEPFSKIALEAASAAAHHQRHEGSRMVEA
                     LNRSEFVDRALRQAVDRESRNLEGGLTLLATVGSAAVFVGLLGTVMGIYYALINLGAS
                     GESSISAVAGPVGQALIMTAIGLFAAIPAVIAYNAYSRANRTTIAHFDEFAHDLHDFF
                     STGARIEGNAPAMARPAAATPPPPAMKK"
BASE COUNT         1343 a         2931 c         3059 g         1631 t
ORIGIN      
        1 gcgaagcagt agatgcagcc gtgctcgcag ccgcggtagg gattgatcga ctgctcgaat
       61 gccacgtcgg gcgaatcgtt gcggctgatg atcgagcgcg cgcgctcttc gttcacttca
      121 gtgcgcggcc gcggcgcgga ctcgtcgagc aacgcactct gccatccgtc gtcctcggcg
      181 tagtggcgca tggtttcgaa gcgcccctcc ggattggtgg cggcgccgcg gcccttgatg
      241 cgcacgggtg atgtcgggtc catggtgcga tccagcatac gccggtccgt ctcggcgcct
      301 gcgacatctc ctggtcgtgc ttgacggaag gtgtgtgtat tggcatattg atacacatgg
      361 acgcatcgct gctggtcatc cagccgacct ccgccgagcc gatctaccgg caaatcgtcg
      421 agcaactgcg ccggctgatc gcgggcggcc agctcgtgcc gggcgacttg ctgccctcgg
      481 tgcgcgaggt ggcggggttc catgcggtga acccgatgac ggtgtcgcgc gcctacagcc
      541 tcgccgaagg cgagggcctc ctgcagcgac tgcgcggcaa gggcatggcg gtggcggcgc
      601 agcgcaatgc cagtcacaac ccggctcagc gcctcgcctt gttggaacct caattggccg
      661 cgctcgccag gcaggcacgc gaactggaac ttcccgccga acaggttttg cgccgactcg
      721 acaaaatgat ggggagcaaa gcaccatgaa tgctgttgca cgtaatgtct cggccgaatc
      781 ggccgtaccg ctgcaggtcg gcggactcgt ccaccgctac gactcgaagc tcgtgctcga
      841 ccaggtcagc ctgtcgctgg aacccggcag cgtgctcggc ctgatcggcc gcaacggcgc
      901 gggcaagtcc accctgatcc gcgcgatgct gggactgctg gagcccgacg ccggcagcgc
      961 tttcgtgttc ggggagccgg cgttgaaact gcccgacgac gtcaaggagc ggctgggtta
     1021 cgtgccgcag cagccggacg ccctggcgtg gctgaccgcc acccagatgt tcgactacct
     1081 gtcgcgtttc tacccgcgct gggatggccg gttcgtcgag aacacgatgc aacgctggcg
     1141 gatcgcgggc gacaaggtgc tggcgaaact ttcgcccggc gagcgccagc gcgtggacct
     1201 cgtgcgcgca ttggcatcgc agcccgaatt gctggtgctg gatgagccgg cggccgcgct
     1261 cgatccggtt gcgcggcgcg agctgttgcg cgagatcgcg ttgcgcgccg gcgagtccgg
     1321 caccacggtg ctgttttcga cccacatcgt ctccgacctc gagcgcgtgg cgtcggacat
     1381 cgccttcctg cacgagggga aactgctgtt gcactgcccg gtggacgaca ccaaggagcg
     1441 ctacgcgcgg ctgtggctgc ccgccaacgt cgcctatgcc gcgcccccat ccgtgttcgg
     1501 caagcgccgg cacgaggatg gcagcgtcag cgtgatcgtc gaacgcgatt cggacggcgc
     1561 gtggcccgac gcagcgcgac tgcccgcagc acggatcgac gcgctcggac tggaagacct
     1621 gttcgtggag atcgcggagc catgaacact tccgtcgccg acgtgccggc cagccacaag
     1681 gggacatcga tgcgcttcat cgacattctc gtcgcccccc gctatgccac atcggcctcc
     1741 acgcgttggg ccagcgtggg attcttcgtc ctgctggcgg gcgccgccgt ggtgatcgga
     1801 atcatttcgc acgacccgaa ggcgcgattc gccgcgttga tttgctacac cttcgggatc
     1861 accatggtgt ggtggctatg gctgtcgggc ctggtgctga tcgcccgcga cggacgtgga
     1921 ctcggcctgc cccgcgccgg ccgcgagagc gcgtacgcag catgcctgta cgcggccgcg
     1981 atggtggccg cgcccgcgtt gctggtcgcc gcgagcggcg ggagcgtggc cgccgccgtg
     2041 ctgtttccgg cgttggcgat cgcgggcagc ctcgcgtgga cgctgctgcc gcgctggctt
     2101 gcgatgtggt tcggcttcat gccggccatc tacatcggcc tccacaacgc gttccatgtg
     2161 ccctcgccgc tcgatccccg tttccgtaat tgggcatggc tgacgctggc gatgctggtg
     2221 gtggtggtcg tgctccgctg gcggcaaatg gcacgcggcg aggacaacga cgacagccgc
     2281 tggtcgagcg cgatgatcct gcagatgcgc cgccacgcgg tgagctgcga ctggtggtcg
     2341 atggaccgca actgggcctg gcggcgctcg cgcggacgca agatcgacgt cgatttccgc
     2401 gggatcggtg ccgcgaatcc ggccttggcc atccgcgtgg cgctcggcga ctggtacgtt
     2461 ccgcaatcct ggcggagccg tgccgggtcg ttggcccgcg tcctgctgcc actgctgctg
     2521 ttcattccgc tgatggtgtt catgaacatc ggtcgctacg tttcgctcca cacggtgtgg
     2581 aaggtcatcg ggatcagtgg cggcctgtgg attggcctgt tcggttccgc gatgctggcc
     2641 ctcaccgtcg ccgcgcgggt gcaacgccgc tggcgcggtt cggctgacct ggggctgctg
     2701 gcattgctcc cgggcctcgg cgcggcggcc gcatcgcatg tcctgcgcgc gacattcctc
     2761 ggaccggcca ttgcctttgt tgcgttgtgg ctatgcatgg tggcggggat tgcactggcg
     2821 gcgcccggcg tccttgcgac gttgttggcc acgctgttcg tgctcgaaat ggccgcgctg
     2881 atggcgatgg ccggactgcg ggcgatggcg gggttgagga tgcggttcat ggtgaaagtc
     2941 gcgatcggcg tggtcctgat cgtgctcggc gattcgggct tcaccctggc gttcgtatcg
     3001 cccacgcagg cttcggccgc actggtcgga gccgtcgtgc tgggatggct cctgctaatt
     3061 gcgtggacgg tgtggcgggg gtggctggca tggcgtctgc tgcagcgccg accgcacccc
     3121 ttcctggcga ccccgccgca gtagggcagg cccgccgatc gggtcgatcc tgtctcccga
     3181 actgtgatcg gcggccctca tcgccctgct gcctcgtacg gtatgatccg cccccggggc
     3241 tacgtggtgt gagtgcatga tctggggcct ggccatcgtt tcgctgcttg cgttcgcgct
     3301 ggcgttcacc agccattcgc cgggcttgat ggggctcggt gtcgtggtgg gtttcctgtg
     3361 cggcatcgcc gccgcgctgg ttttcatcga ccgtcacctg cgtgccagct cgcgtcccga
     3421 gcacatgacc gagcgcgaga tctccgcgct gcgcgacacg atccgcaagc ccgaagagtc
     3481 ccggcgcaag ctcccgcccg gcccctccgc ctgacggtac ctcgcgacca ggcgcctccg
     3541 gtttgctacg cttgggtcct tgcgtggcct ggaggagacg tgcgatgaac atggtgcatc
     3601 cggttccgtc ggcattcgcc gccgccgagc ggatcaagcc cgacgagtac gaacgccgct
     3661 acgccgaatc gctgcgcgac ccggaggcgt tctgggccga ggccgcgaaa cggttggact
     3721 ggatcaagcc gccgactcaa atccgcgacg tttccttcga ccaggaggac ctgcacatcc
     3781 gctggtacgc cgacggggtg ctgaacccca ccgtcagttg cctcgatcgc cacctcgaca
     3841 agcgcggcga caagaccgcg ctgctgttcg agggcgacga tccctccgtt tcccgcaaga
     3901 tcagctatcg cgaactgcac gccgaggtgt gccggttcgc caatgcgctg cgcaacctcg
     3961 gggtggtgaa gggcgatcgc atcgcgatct acatgccgat ggttccggaa gcctgcatag
     4021 cgatgctggc ctgcgcgcgg atcggcgcga tccattcggt agtgttcggc ggcttctcgc
     4081 ctgacgcgct ggccgggcgc atcgccgatt cggcgtgcaa gctggtgatc accgccgacg
     4141 agggcgtgcg cgccaacaag aagatcccgt tgaaggcgaa cgtcgacgag gcgctgaccc
     4201 gccccggaac caccagcatc gaaacggtgg tcgtcgtgcg ccgcaccggc ggggcggtga
     4261 acatgcagtc gccgcgtgac cgctggtggg atgcgctggt cgacggccaa cccgaccagt
     4321 gcgagcccga acccatgaac gcggaggatc cgctgttcat cctttatacc tccggcagca
     4381 ccggcaagcc caagggcgtg ctgcacaccg tcggcggcta catggtgtgg gtgtcgctga
     4441 cccacgaggc aatcttcgac ctgcgcgagg acgacatcta ctggtgcacc gccgacgtcg
     4501 gctgggtcac cggccatagc tacatcgtgt acgggccgct ggcgaacggc gcgacctcgc
     4561 tggtgttcga gggcgtgccg acctggcccg acgcctcgcg catctggcag gtgtgcgaca
     4621 agcaccaggt gacgatcctc tacaccgcgc cgaccgccat ccgcgccctg atgcgcgctg
     4681 gcgacgaacc ggtgaagcag acttcgcgcg agtcgctgcg gttgctcggg tcggtgggcg
     4741 aaccgatcaa cccggaagcc tgggagtggt atttcaacgt ggtcggtgaa ggccgttcgc
     4801 ccatcgtgga cacctggtgg caaaccgaaa ccggcgggat catgatctgc ccgctgccgg
     4861 gctcgacaac cctgaaaccc ggcgcggcga tgaagccatt tttcggtatc cggcccgcga
     4921 tcctcgatgc cggcgggggc gtcatcgaag gcgaggggtc cggcaacctc gtgatcaccg
     4981 agtcctggcc cgggcagatg cgcacggtgt atggcgacca ccagcgcttc atcgaaacct
     5041 atttcaagat gtacccgggc gtgtacacca cgggcgacgg cgccgagcgc gatgccgacg
     5101 gcgactggaa gatcaccggc cgggtcgacg acgtcatcaa cgtgtccggc caccggatcg
     5161 gtacggccga agtcgaaagc gccatgatgg ccgacctgcg cgtggccgaa gccgcgatcg
     5221 tcggttgccc gcacgacatc aagggcacca ccctctatgc cttcgtgacg cccaaggaag
     5281 gggtggcgcc gagcgacgcg ctgaagaagg aactcatcgc cggcatccgc gaacggatcg
     5341 gcgcattcgc ggcccccgaa tacttgcaat gggcaccggc gctcccgaag acgcgctcgg
     5401 ccaagaccat gcgccggatc ctgcgcaaga tcgccgaagc cggcgaccac gaagtcgacc
     5461 tctccgcgct gggtgatacc tcgacccttg ccgatccgtc gatcgtcaag cgcctgtacg
     5521 acgaacgcgt gcggcactga tacgagtacg cgaggacgcc gttcgatcgt gcacgagctg
     5581 acgtttgccg atgcggaccc cgcggccgtc gcgggtcagc tcgatgatgg atcgatccac
     5641 ctctggcgca tgccttatgc ccagtcgcag gggcgggcgc cgttgctgca gctgctgggc
     5701 gcctatctcg gaagggccgc gtcggaggtc tccctggtgg agggcccgca cggcaagccc
     5761 ggcttgcctg gcagcaaatc gcaacgtggc gaaacgcgcc ggctcgagtt caactggtcg
     5821 cacagcggag gcagcgcgct ggtcgcgctt gcccgtggcc gcgcggtcgg tgttgacatc
     5881 gaacggttgg gtaggcgcat gcgcacgctg gaacttgcgc gccgcttctt cgatcccgcc
     5941 gagggcgacg cgctcgaatc gctggcgttc tccgagcgcg attccgcctt catcggactg
     6001 tggtgcgcca aggaggcggt actgaaggcg gcgggcgaag gcctgtcgtt cgggctcgca
     6061 cggctggtct tcgtcccgca tccgcgcgcg ggctggaagc ttgaccgcgt ggattccgcg
     6121 ttgggcggcg ggaacgaatg gcggatcgtc ggcttcgagc cggcgccggg ctaccacggc
     6181 gcgctcgcct ggcacgggcc agagccgcgg atctccgctt tccaggagcg catggcgacc
     6241 ggaggctgaa cggttccccc ggcgacggaa ctcgcccgaa ttccgcgggt ctgactgtta
     6301 cgggcagtct ccccgtgggt tcgccatcag acggtgggcc atgcactctc cggcgcgaag
     6361 tcccccttcg cgccgctttt tttgcgtgtc gttgtgcgat catcgccatt tgccacgttc
     6421 caaagccgtc atgtccagtc gagttttcga ggccgtggaa cgtcaggacg gttcttggcc
     6481 cggctcttcg cgaagctatt ccgctgacag gcttcgatga ccgtactgag cgtcaacctc
     6541 aacaagattg ccgtgctgcg caatgcgcgc ggcggcgccg agcccagcct cgcgcgggcg
     6601 gccgacacct gcatcgccgc cggctgcggc ggcatcaccg tgcatccccg cccggatcgc
     6661 cggcacatta cccccgaaga cgtggcgctg ctggcggaac tgacccgcgg ccgggtggaa
     6721 ttcaacatcg agggcaatcc cttcgcgccg ccgcgtcccg gttaccctgg catgctcgaa
     6781 ctggtgcggc aggcgcgacc ggcgcaggcg acgctggttc ccgattccga cggccagctc
     6841 acttccgacc atgggttcga cctggtgcgc gacgcgccga agttggcgcc gctggtctcc
     6901 gaactggttg ccacaggctg ccgggtttcg ctgttcgtcg atgcagggag cgcggagggt
     6961 ttcgatcgtg cccgtgacct cggcgcggcg cgggtcgaac tctataccgg tccctatgcc
     7021 cgcgccttcg cggaaggcga cgccaccgac gagctgtcgc gttgcatgga tacggcaagg
     7081 cgggccggga tcgcgggcct cgcggtgaat gcgggccacg acctcaacca gcaaaacctc
     7141 ggtgccttcc ggcgcgcaat tcccggactc gccgaagtct cgatcggcca cgcactgatc
     7201 ggcgaggcac tctacgaggg cctcgatcgc accgtccgca attacgtggc aatcctgtcg
     7261 cgcgagtcgg gccccagcta ttgatgctga acgcaggcgc gtgacgattg gtcgccttgg
     7321 cgagagcttt tagacgcgga tcaaagccga aatagacgga taggaggctt ttgtccagct
     7381 cgtatccgcg tttatccgcg tcgaatgttt ttcgtcactt gggcacgcaa gcctcgctgg
     7441 ctgcgcaatc caggagggtg gcgatgaccg cggacagcgg gacggaccgc ccgcgtacga
     7501 aggatgacgc gtgcgtcagt ggaccaccga ccccgggacc gccttggcgt tgccctgggt
     7561 gatgaagccg atgtgcacca tgcccgcatc cttcgcttcc tgcatgacct tccagatgat
     7621 cttgtactgg gtggtcttgt cggcgcggat gttcaattcg ggctggggct gcacggtcgc
     7681 ggcggcacgc aggcgcgcgc tcaacaccgc atcggaaatc tggttgtcgt tccagtacag
     7741 ggtgccatcg ggttcgaccg cgaggtcgat cggcggcacc tccaccacgg acggcttggt
     7801 ttcggggttg gcccgcggca ggtcgacctt caccttgtgc gacatcaggg gtgccgtgat
     7861 catgaagatg atcagcagca ccagcatcac gtcagccagc ggcgtcacgt tgatttcggc
     7921 cttggggata tcgctgccgg tgttgtcgcc cgcgctcatt gccatggcgt cattccctca
     7981 cttctcgaag ccgatcttct gcacgttgta gcgcttggct tcggccatta cttgtgccag
     8041 gtagttgtac tgggtttcgt cggtggcctt gatgtgcact tgcggctgca tgtcgatcgt
     8101 cttggcgccg atcgtcgcga actgcgcctg cagttcgacg ctgctgatcg gcaggttgtc
     8161 ccacgtcatc gcgccatcgg cgcggatgaa cagcgtcttc ggcgtcggtg ggttctccgg
     8221 cggcttgacg ttggggttcg gctgcggcag gtcgatcttg atgttgtgct gcaccagggg
     8281 cgcggtgatc atgaagatga ccagcagcac cagcatcacg tcgatcagtg gcgtgatgtt
     8341 gatttcggcg accgggccgc cgccgctatt ggtactcatg gccatgacga aaccctctta
     8401 cttcttcatc gccggcgggg gtggcgtagc cgcggccggg cgggccatcg ccggcgcatt
     8461 gccctcgatg cgcgcaccgg tcgagaagaa gtcgtgcagg tcgtgcgcga attcgtcgaa
     8521 gtgcgcgatc gtggtgcggt tggcgcgcga gtacgcgttg taggcgatga ccgccgggat
     8581 cgcggcgaac aggccgatgg ccgtcatgat cagcgcctgg ccgaccggac ccgccaccgc
     8641 ggagatcgac gactcgccgc tggcgccgag gttgatcagc gcgtaataga tgcccatcac
     8701 ggtaccgagc aggccgacga acaccgcggc cgagccgacg gtcgcgagca gcgtgaggcc
     8761 gccttcgagg ttgcggcttt cgcgatcgac cgcctggcgc agcgcacggt ccacgaactc
     8821 ggaacggttc agcgcttcca ccatccggct gccttcgtgg cgctggtggt gagcggcggc
     8881 cgaggcggct tcgagcgcga tcttcgagaa cggctccgag cgcggctgct tctccatcac
     8941 ccgcacggca tcctgcgcgg aggg
//
LOCUS       DASSNH010000019         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_483439, whole genome shotgun sequence.
ACCESSION   DASSNH010000019 DASSNH010000000
VERSION     DASSNH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483439"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1482
                     /locus_tag="VFK00_00510"
     CDS             193..1482
                     /locus_tag="VFK00_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7591470.1"
                     /translation="MAKTKVKRRIFWAALGAIGAAVVVIISLNLSPREQELQHRLQHV
                     SAVDSVQFRREIGTLLGASVTEDNAIADYQNGAEYFPAMLAAIRGAQRSVNMETYIYW
                     SGDIARAFADALIERARAGVKVHVLADWVGSSKMKHSLVERLRAGGVRFEYFHPLHWY
                     TLDRINNRTHRKLLIVDGKIAFNGGAGIADAWNGNATRPDHWRDMEFRVEGPAASQMQ
                     AIFADNWLAATGEVLLGDAYYPAPPRTGNTAVQAFASSPEGGSINMQLLYLMAINGAR
                     HSIDLEAAYFIPDELTQTALLDALHRGVKVRIVVPGGHVDSPLARSASQQTWGMFMKA
                     GARFFEFQPTLFHNKLMVVDSYLTIGGSANFDDRSFKLNDESNLNIPDRAFATHMTGV
                     IDGDIARSREVTLAQWQDRPWTRRVADWLSTLGSSQL"
     gene            1661..>2158
                     /locus_tag="VFK00_00515"
     CDS             1661..>2158
                     /locus_tag="VFK00_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591471.1"
                     /translation="MKRYLSLLAIAWLTCLATPAVAGDAHGAPANRVDGDVGTYEHAS
                     EAAGGAALWETLDGLLGNAAARQAQPAAMWMGSGYTSGYAVSGRTCKLLERESRLFKS
                     GLDTGKYTPADLSRASIAEHAKKAGIPRYSPAFWTIATSSLVAANPANVDLPAQKMGE
                     KMYDLC"
BASE COUNT          393 a          675 c          736 g          354 t
ORIGIN      
        1 cgcacgtttc gatcagcccg attgggtgcc ggccattgca caccatccgc cggatcagta
       61 ccttctgctg gctgtcgggc acttcgcaat atgggacagt gcgggagcag gtggatcggg
      121 gccgtgccgg ctgcgcgtag ctccgtggcc gatgcgcagt ttggacaggc ggcagaagaa
      181 ggatctggac ccatggcgaa gacgaaggtc aagcggcgaa tcttctgggc ggcgctgggc
      241 gcaatcggcg cggcggtcgt cgtcatcatt tcgctcaacc tgtcgccgcg ggaacaggag
      301 ttgcagcatc ggctgcagca cgtcagcgcc gtggacagcg tgcagttccg gcgcgagatc
      361 ggaacgctgc ttggcgcgag cgtcaccgaa gacaacgcca tagccgacta ccagaacggc
      421 gcggaatact ttcccgcgat gctggcggcg atccggggcg cgcagcgcag cgtcaacatg
      481 gaaacctaca tctactggtc cggcgacata gccagggcat tcgccgacgc cttgatcgag
      541 cgcgcccgcg ccggggtgaa agtgcacgtg ctcgcggatt gggtgggatc ctcgaaaatg
      601 aagcattcgc tggtggagcg gcttcgcgcc ggtggcgtgc gcttcgagta cttccatccg
      661 ttgcactggt acacgctcga ccggatcaac aaccgcacgc atcgcaaact gctgatcgtc
      721 gatggcaaga tcgccttcaa cggaggtgcg ggcattgcgg acgcgtggaa tgggaacgcc
      781 acccggccgg atcactggcg cgacatggaa tttcgggtgg aagggcccgc tgcgtcgcag
      841 atgcaggcga ttttcgcgga caactggctg gccgccaccg gcgaagtcct gctgggggac
      901 gcttactatc ctgcgccgcc gcggaccggc aacaccgcgg tgcaggcatt cgccagttcc
      961 cccgagggcg gcagcatcaa catgcagttg ctgtacctga tggcgatcaa tggcgcccgg
     1021 cacagcatcg acctggaggc cgcgtatttc atcccggacg agctgacgca gaccgcgctg
     1081 ctcgacgcgc tgcatcgtgg cgtgaaggtg cgcatcgtcg tgcccggcgg gcacgtggac
     1141 agcccgctcg cgcgcagtgc ctcgcagcag acgtggggca tgttcatgaa ggcgggggcg
     1201 cgctttttcg agttccagcc gacgctgttc cacaacaagc tgatggtggt ggacagctat
     1261 ctgacgatcg gcggctcggc caacttcgac gatcgctcgt tcaagctcaa cgacgagtcc
     1321 aatctcaaca tccccgatcg cgcattcgcc acgcacatga ccggagtcat cgacggcgac
     1381 atcgctcgat cgcgcgaagt cacgctcgcg caatggcagg atcgcccatg gacgcgccgc
     1441 gtggcggact ggttgtcgac gctgggctcg tcgcagctct gagggcaacc cgggggcccg
     1501 cttcgaccac gacgggcacg ctagcggctg cggaaccgtg gcgtattcga cccgctttcc
     1561 agaaagtgaa cccgaccccg ttttcaccgg cccgggtcgt gcgatggtgc ggccaacgaa
     1621 acgggatcgt ccgccgatgc ctggcgggcg aggagatccg atgaagcggt atctgtcttt
     1681 gctggccatc gcgtggctca cctgcctggc gacgcccgcc gttgccggtg acgcccatgg
     1741 cgcgccggcc aaccgggtgg acggcgacgt cggcacgtac gagcacgcat ccgaagcggc
     1801 gggcggcgca gcgttgtggg aaacgctcga cggattgctg gggaacgcgg cggcacggca
     1861 ggcgcaaccg gcggccatgt ggatgggcag cggctacacc agtggctatg ccgtcagtgg
     1921 caggacctgc aaactgctgg agcgagagtc gcggttgttc aagagtggcc tggacaccgg
     1981 caagtacacc cccgcggacc tgtccagggc aagcattgcc gaacatgcga agaaggcggg
     2041 catccccagg tattcgccgg cgttctggac catcgcgacc agcagcctgg ttgccgccaa
     2101 tcccgccaac gtggacctgc ctgcgcagaa aatgggcgag aagatgtacg acttgtgc
//
LOCUS       DASSNH010000020        23098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_352788, whole genome shotgun sequence.
ACCESSION   DASSNH010000020 DASSNH010000000
VERSION     DASSNH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 23098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23098
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352788"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="VFK00_00520"
     CDS             <1..1929
                     /locus_tag="VFK00_00520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7591472.1"
                     /translation="IARREARHFPHLETGLAPVGVPRHRVDNHAEALQKRGIVAELSH
                     RHVLRAAAVYVGAVWALSQGIAQLAPVFDAPTWITRWFVIACAVGFPFWLAFAWYYKL
                     TPEGFKRETDVAVDDPAFHRATGRKLDFWIIGVMAVAIVLLVTNTFVLHRDVNSAASA
                     ADAETIAAELAKVPFQSVAVLPLANRGDSKQQYFSDGLSEELISDLTQVNGLKVIGSY
                     SSFKFRDSADSPAQIGAALGVAHLILGSVQQQGNRIRVTVNMIRAKDGASVWSNSYDE
                     QLEDVFAIQSRIGDAVAKALKIKLLGNTIVSSDRPPSGSVEAYKLMLQGHGLVQQYTV
                     SGFNQGISLLEQALKIDPDYAYAWGVLSTALINQGQTVLSGEARLQAYAKARIAADKQ
                     QELAPDAADTHLTRGYLLQNVDLDPMGALAEFRRGVELAPNDGRAMEFLAYGLASAGQ
                     LRAAVELFRKAIDTNPFNTPWYATLASILLGNGELDAAEQASRKTLELLPGTPGMYAN
                     LALINILRGDAAAAARDAAQEPDPVLGAWARAMALQIDPSRAKADAALRDYVAAHGES
                     EPYFVADLYAVRKQPDEMFQWLERAFGQHDPAFFSLLYDPLPLAYKNDPRFAALCKRA
                     GLPVPGQPLPTMAGVTNQ"
     gene            complement(2167..2919)
                     /locus_tag="VFK00_00525"
     CDS             complement(2167..2919)
                     /locus_tag="VFK00_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7591473.1"
                     /translation="MRNSLLVSAALASGALLFSVGAKAVPIHVGIQAAGGSQVWTSGP
                     IEDPSFVTLSAWEDDGNGWYGVYMTNGQDALGNPGLGVCGSQPGGANSCAVPGETNEI
                     DRLPQQMIELNITEFHNWNSVTITLLSVEQDPFAGLWGASCSLLDPNCTPTLTALEPT
                     CSGPDANGIVSCTYSETYLESLDIDAIWIQPQFDCGRNCGRNFYLGAGDEFGLVLDLN
                     PESVPEPSVLGMFGLGTLLLGLFAGLRRRRTS"
     gene            3315..3419
                     /locus_tag="VFK00_00530"
                     /pseudo
     CDS             3315..3419
                     /locus_tag="VFK00_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3497..3739
                     /locus_tag="VFK00_00535"
     CDS             3497..3739
                     /locus_tag="VFK00_00535"
                     /inference="COORDINATES: protein motif:HMM:NF022058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591474.1"
                     /translation="MDRILAKHELYCTVCENNTGDCQLHNTFAGMHMPIQRYPLTRKP
                     FYTYDPDQCILCGRCVVKACHSPTSINKLRESGWWS"
     gene            complement(3728..3803)
                     /locus_tag="VFK00_00540"
     tRNA            complement(3728..3803)
                     /locus_tag="VFK00_00540"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3768..3770),aa:Ala,seq:cgc)
     gene            complement(4038..5120)
                     /locus_tag="VFK00_00545"
     CDS             complement(4038..5120)
                     /locus_tag="VFK00_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HET7591475.1"
                     /translation="MTAPWIDQPGCLATLDDQDPVGLDTEFMRRNTYFPKLALVQAAH
                     RAEHWLLDPLAYDAGPDVRSLVTGRCCIMHSAGEDMEALAPMLVDVPLRLFDTQIAAA
                     LCGMGPGLSYQKLVAGLLGIEISKDETRSDWLQRPLTASQRDYAELDVAYLAELHGKL
                     SDELARLGRREWHAEDCERLVRRADRRRGTDPQPQKGFPAASDWPPEVQARLRRILLW
                     RDEAARTLDKPRPWLLDDAHALQLAQQPPATPQALFDRVKGQRALRGPQRTELFALLQ
                     APATAGELSELSPIPPAPRGEYKKVIDAMRMAVQAVAARLELPPGLLCPRRLVVEFAT
                     TREWPEGMEGWRRGLLEEVLTPLLPD"
     gene            5210..7129
                     /locus_tag="VFK00_00550"
     CDS             5210..7129
                     /locus_tag="VFK00_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET7591476.1"
                     /translation="MPNPQFITRQHAIGGTIGVLLLVFALVYRFFPAALKVEPAALRS
                     HPATASSAPLVARQPPRKLTPAESELNAGPPISLAPTKVIAARAREGTLGAQVGTGEV
                     AQLLGRAGAAAASGNLAGPGEDTAIALVLQAVQEAPDDAHVKKAVSLLHARLVADAQQ
                     SLAAGDAEAARTNLDALKRLPGAGADVAALADRIDAAGKVQPLLEHASNLVQQGKLEG
                     SGDDTALAVYRKVLALDPENVVARQGLQRVQQASLDRALTAAAQSEDAKADAALADAA
                     AILPSSRALADTRARIATIRSGRADNLMAQAKSALDAGNVDLAQRLADQAAGLGASAD
                     EFLARLKNAREYASYHPGQVFSDHFLDIVGQSPAMVVIPRGSFLMGAASGSGASAEQP
                     QHEVTLATGFAMARTEVTVAEFRAFVRSSGYVPDSKRLGGSSVYDETNGRMQQRAGAD
                     WQDDYAGKRAKGSDPVVNVSWNDAHAYAAWLSRHTGKQYRLPTEAEYEYAERAGSTTL
                     YWWGDVTPKVLVENLTGSGDRSPAHRSWANAFKGYEDGYWGPAPVMSFLANPFGLYDM
                     SGNVSEWVADCWHENYVRAPNDGSAWVNPGCSERVIRGGSWGSAPDQDRSSFRQGAPA
                     EARSGRVGFRVVRNL"
     gene            complement(7140..7892)
                     /locus_tag="VFK00_00555"
     CDS             complement(7140..7892)
                     /locus_tag="VFK00_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET7591477.1"
                     /translation="MIQRGGGGTITKHRAGAPARFFEAEARGLGLLRDAGGLRVPDVL
                     EVDADRITLEDLGQGTPAADYWLRAGSGLGRQHARRGERFGLTHGGFCGDSSQDNTPD
                     DDGWRFFAERRLLPQMRRARDRGLIEGRDAAAIESVCRRLRELVPDLPAVLLHGDLWL
                     GNLHVCGDGSPALIDAGAVHYGWAEAELAMLTLFGSPPDAFFHAYAEGAPLRPDWRER
                     TSVYNLYHLLNHVNLFGAGYLGSLRSALSACR"
     gene            complement(8326..8412)
                     /locus_tag="VFK00_00560"
     tRNA            complement(8326..8412)
                     /locus_tag="VFK00_00560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8376..8378),aa:Leu,seq:caa)
     gene            8623..9000
                     /locus_tag="VFK00_00565"
     CDS             8623..9000
                     /locus_tag="VFK00_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591478.1"
                     /translation="MRSLKLVFFGLAAVGMSAGALAQNAPTQNPPTQPATQAGSKPAG
                     QLGNSGGGLSMGMTPREAQMAFGNMDANHDGFLDRSEFMHNGDSGQRFAGCDVNRDGK
                     LSEAEYVQCSQRPATQDQGNGQP"
     gene            complement(9022..9588)
                     /locus_tag="VFK00_00570"
     CDS             complement(9022..9588)
                     /locus_tag="VFK00_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HET7591479.1"
                     /translation="MGIDFPGRDRMIRAFDAAVQAPDTTATVRGLRSALCDAIHSGEV
                     HLPGFVFEGCDGHYARRELYCSPEHGYCVVAMTWSPGQGTPIHDHAGMWCVEGVWQGA
                     LDVVQYELRDHNEARYRFVAAGAIRAGTGSAGSLIPPHEHHTICNASDRELAISVHVY
                     QNRMTHCSVFEPIGGEWYARGERSLTLD"
     gene            complement(9635..10750)
                     /locus_tag="VFK00_00575"
     CDS             complement(9635..10750)
                     /locus_tag="VFK00_00575"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET7591480.1"
                     /translation="MADARQYFTLPSPFKMKRGGELRGGQVAYEAWGTLNAARDNAVL
                     ILTGLSPSAHAAHNAIDDSPGWWEDIVGPGKAIDSNRWFVICMNSLGSCKGSSGPASP
                     NPVTGEPWRLDFPDLSLEDIGDAGYELAHGLGIERLAALVGCSMGGMSALGSLAHHPG
                     FARAHVSVCTAPRAQPFAIAIRSLQREAIRLDPNWNEGRYDDDHYPETGMNIARKLGV
                     ITYRSSLEWRGRFGRIRLDPGDREDDPFGAEFQIESYLNAHARRFTRSFDPNCYLYLS
                     RASDWFDLAEYGGAPDSSTAVRNALAKMKLQRALVIGVETDILFPLEQQQQLAEDLTA
                     TGTDTTFVGLDSPQGHDAFLVDTGRFGAAIGAFMATL"
     gene            complement(10948..12018)
                     /locus_tag="VFK00_00580"
     CDS             complement(10948..12018)
                     /locus_tag="VFK00_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7591481.1"
                     /translation="MTTRFLIIAAAMVVVALACVLVPMLRSARREGRPRTPFVLALLL
                     ALAVPPAVLAMYLAIGTPQALQPAVPDNRADLAAATEQLQASLAKKPGDAQGWALLAQ
                     AYAAMGQPQQALDALDHLLKLKPDDPDAMVAWAEARAETSPSHRIDDASRDQLQRALQ
                     IDPGHQRALWLLGISDFQRGQYDDAATRWRTLLPLLQPGSKVADAVQQELAAAEARAG
                     GGVRTGELAAADPAPAGSSGAAAGEIALTVSVKLAPDLASKVQPGDTLFVFARAVDGP
                     PMPLAVARLKASQLPAKVTLTDAMAMTPALTLSKFPKVQVAARISKSGNAMPQAGDLE
                     SASVEIATGSHAPVALTIDRVD"
     gene            complement(12015..12437)
                     /locus_tag="VFK00_00585"
     CDS             complement(12015..12437)
                     /locus_tag="VFK00_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HET7591482.1"
                     /translation="MAVVVVAFALAFSAGAFAIDPLPFQSTAQRERFQHLTGELRCMV
                     CQDESLLASNADFAKQLRRQIFDMMQQGKSDEEIKDWLVARYSTFILYDPPLAPSTIA
                     LWFGTPAILLAGGIVVVVLLRRRTRPAGSIAEDPGDDW"
     gene            complement(12614..13156)
                     /locus_tag="VFK00_00590"
     CDS             complement(12614..13156)
                     /locus_tag="VFK00_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HET7591483.1"
                     /translation="MGRFVPLIVFAALVAFFVVGLVWNQTHDARFVPSPLIDKPAPAF
                     RLPKLEQPTQFVSKADLLGKPYLINVFASWCFACGDEHPVLMAYRDRFGIPLIGYDYK
                     DAPRDARAWLERHGNPYHEVIADQSGDTAIDFGVYGAPETYLIDAKGIIRYKHIGPLT
                     PDVIATELEPRIRELEDQGT"
     gene            complement(13177..15159)
                     /locus_tag="VFK00_00595"
     CDS             complement(13177..15159)
                     /locus_tag="VFK00_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET7591484.1"
                     /translation="MNPELGQVALILALLLSGLQCLLPIVGAWRGSKPLMATAPSLAI
                     GQAVFVALAFGILAWAFWTNDFSVAYVAQNSNLSLPWYYKLSGVWGAHEGSLLLWMLI
                     LNLWTVAMVALGGRLPKAFFSRVIGVLGFVAFGFLLYMITTSNPFLRLFPVPPNGADL
                     NPVLQDPGLVFHPPMLYMGYVGFSVAFSFAIASLLGGELKSTWVRWARPWTNVAWGFL
                     TLGIVAGSAWAYYELGWGGWWFWDPVENASFMPWLIGAALIHAQAITDKRDGLRAWTL
                     LLSIFGFAFSLLGTFLVRSGLLTSVHAFAFAPARGVFILVYLAVVVGGSLLLYALRAP
                     KVTGGKSFALASRETMMVIGNLMLTVACAMVLLGTLYPLVGEALDIGRISVGPPYFGP
                     LFLLLMTPLVLALPFGPLSRWGKADLKVWRNALIRIVALAGAAGIIGFALGARSFYEL
                     LGITAAVWVMAGVALFAIKRWREAPRGKRYPFEMLGMMLAHFGIGVFIAGAILTRSMS
                     VEQDVSMKPGASEEVGAYDFHFNGVVETEGPNYRAQQGTVVITRNGRHVVTLHPQKRT
                     YAGGQVQTEAAIEAGVFRDLYVSLGDSIGNGAWSLRVYDKPFVRWIWGGGVLMMLGGF
                     TVVLDKRFRLRRAVEPETVRATPEPVVPPKLAEGEA"
     gene            complement(15238..15762)
                     /gene="ccmE"
                     /locus_tag="VFK00_00600"
     CDS             complement(15238..15762)
                     /gene="ccmE"
                     /locus_tag="VFK00_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET7591485.1"
                     /translation="MNPVRKRRLIVALLILGAVGIATTLSVFALQRNVTYLYSPSEVD
                     AGKAPAGHPFRLGGIVEEGSVHRVPDTLTTDFVITDRFQSIPVSYTGILPDLFRVGQS
                     TITTGTMVDGKFVATKVLAKHDATYMPPQVEQAIAKAKEEHRIPERGTGDRGPGTGQH
                     RSAAGDSDGPHGTE"
     gene            complement(15759..15953)
                     /gene="ccmD"
                     /locus_tag="VFK00_00605"
     CDS             complement(15759..15953)
                     /gene="ccmD"
                     /locus_tag="VFK00_00605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HET7591486.1"
                     /translation="MAHWLAMGGYAVYVWPAYAVFFAVLAWDWFAPALRRRRLTRELR
                     GRIARERIRNTRAARAEAST"
     gene            complement(15955..16740)
                     /locus_tag="VFK00_00610"
     CDS             complement(15955..16740)
                     /locus_tag="VFK00_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HET7591487.1"
                     /translation="MSRSYLPHWMHRLGSPPTFYRFAGALRPWLMAAALVAAAIGLYG
                     GLVLAPPDYQQGDAYRIIFIHVPSAWMSLFVYACMAVAGLVALVWRVKLAEVIAMECA
                     PAGAAFTLVTLATGSLWGKPMWGTWWTWDARLTSELVLFFLYLGVIGLYHAFEDRRRG
                     ARAASLLALVGIVNLPIVHYSVQWWNTLHQGSTVDWMNLGQSSIAPAMLWPLLVMALA
                     THLYFFASILGRTRTGLLSLEGGKQWVRELVDGQPAQTTGAPT"
     gene            complement(16925..17668)
                     /gene="ccmB"
                     /locus_tag="VFK00_00615"
     CDS             complement(16925..17668)
                     /gene="ccmB"
                     /locus_tag="VFK00_00615"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET7591488.1"
                     /translation="MITAVQPVTDERPVAPAARPRPAGTWHACGALLRRDFLLAWRRR
                     GELFLPLLYAVIVTALFPFALGPDPQLLGRIAGGVLLVIVVLAMLPALESMFRQDLDD
                     GTLEQWLLAPQPLALLVLTRVIAHWLTTVLPLLIVMPLLGALLDLPADVLPVLMLALL
                     LATPLLALLGAVLSALAAGARGSGILLALMLLPLAVPVVIFAAGAIAAVQSGMPYLAP
                     LSWLAAALVLAIVLAPLACAAALRIAVEA"
     gene            complement(17672..18307)
                     /gene="ccmA"
                     /locus_tag="VFK00_00620"
     CDS             complement(17672..18307)
                     /gene="ccmA"
                     /locus_tag="VFK00_00620"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HET7591489.1"
                     /translation="MSPSASPAVPLLAAHRVAFARNDEPVFGPLDFNVDRGETLLVEG
                     DNGSGKTTLLRVLAGMLPGEGEIRFDGTPASRDSRNGRVLLLGHRLGMNDSLSARENL
                     AFLTGLYGTHAGATVESALAAVGIEGWIDEPLRNLSAGQKKRVGLARLLLLPGDLWLL
                     DEPYANLDRHGIELVNALIAEHCAGGGAALVTSHGAVRFADPNARHIRLAA"
     gene            complement(18422..18787)
                     /locus_tag="VFK00_00625"
     CDS             complement(18422..18787)
                     /locus_tag="VFK00_00625"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HET7591490.1"
                     /translation="MRTTWGGYVVIRCGEPLPAPLLALLGRAGDPWGYITAWNPAGVS
                     LPAADNRIRQRRLRDQLRDEGHRYFIGIGVGPNDWREPSLFVPGVAFEQLDSLGRAFG
                     QLGIVRGTGTGIAELRVLV"
     gene            complement(18843..20228)
                     /locus_tag="VFK00_00630"
     CDS             complement(18843..20228)
                     /locus_tag="VFK00_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7591491.1"
                     /translation="MTHPATSSRYTIIGAGLVGSLLATLLAQRGFAVEMFERRPDPRA
                     HGFLGGRSINLALAERGLDALRQAGLADAVLAQAVMMRGRMVHAPDGSTNLQRYGLDD
                     SEVIRSVSRGGLNILLLDAAEGAGATLHFEAGLADVDFERNTITLAGLDGTPRQHAAG
                     LLLGCDGAGSALRVAMSKTTDLGERIEPLGHGYKELEIPPLDSASPAPGFAIEPNALH
                     IWPRGNYMCIALPNTEGNFTVTLFLPSEGVFPSFATLPDIAAARRLFEGQFADLLALM
                     PAFDADWADHPIGQLATLYLDRWHLDGRALLLGDAAHAIVPFHGQGMNAGFEDAAALA
                     DLLAAQPGDPGGMFAAFEARRKPNADAIAAMSLANYVEMRDGVADPHYLLKRELAAAL
                     TRRVPDRFMSRYRMVSFTTLPYATCVARDRQQTALMETILAGHESIEGIDLDAAAARA
                     RADLPLLASSG"
     gene            complement(20475..21761)
                     /gene="kynU"
                     /locus_tag="VFK00_00635"
     CDS             complement(20475..21761)
                     /gene="kynU"
                     /locus_tag="VFK00_00635"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET7591492.1"
                     /translation="MIASHEFRADHAWARIQDEADPLHGLRDEFLIPPHGDGTQTYLV
                     GNSLGLQPRGVRQALLDELDDWARLGVEGHVHGRHPWLPYHREVRDNLATLVGAEPSE
                     VVAMNSLTVNLHLLLVSFYRPTAQRHAILIERHAFPSDRDLAASQIRFHGYDPREALI
                     ELDGDEPGGAISMQSIERALAEHGSRIALVLLPGVQYLTGQAFDLAVITRLARAQGCI
                     VGFDCAHAAGNIDLHLHDSGCDFAAWCSYKYLNAGPGAVAGAFVHARHAGAHLPRFEG
                     WWGRNEATRFEMRPEFDPAAGADAWQLSNPPILALAPLRVSLELFQRAGMSNLRRKSR
                     RLTAYLAWLIETQLGDVLEILTPDEIDRRGAQLSVRVRAGRDAGQSLFGHLVAQGVLG
                     DWREPDVMRIAPTPLYNTFADCLRYAEAVSAWHVPG"
     gene            complement(22049..22561)
                     /locus_tag="VFK00_00640"
     CDS             complement(22049..22561)
                     /locus_tag="VFK00_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591493.1"
                     /translation="MNRKIQSLPVVLAALLMAAAPAYAADYSLAGGAVKFSAPDAWPV
                     LMEKQDGARQFVALQVKDPANPNALARITVTTEQVDGLQGFQSFLSTGTERARKLPGY
                     AAVNMPGSTSSLRYTATENREKNAYTEHYAYRSNLAIQVRCIRPENAPVDWRNTFDAG
                     CQSIVTTVER"
     gene            complement(22558..>23098)
                     /locus_tag="VFK00_00645"
     CDS             complement(22558..>23098)
                     /locus_tag="VFK00_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7591494.1"
                     /translation="AAADLGAAILIHPWDMMGKETMPKYWLPWLVGMPAEQSRAACCL
                     IFGGVLERLPGLRVAFAHGGGSFPFTIGRIEHGFRMRPDLVAVDNSREPREYFQRLFF
                     DSCVHDPQALRYLLDVVGADRVMLGTDYPFPLGEQHPGSGIEALELRPAQRERLYHGA
                     ALEWLGLPAQRFTTNAEHQ"
BASE COUNT         3948 a         7660 c         7850 g         3640 t
ORIGIN      
        1 atcgcgcggc gcgaagcccg acacttcccg catctggaga ccggcctggc accggtggga
       61 gtcccgaggc acagagtgga caaccatgcc gaggcattgc aaaagcgcgg gatcgtcgcc
      121 gaactcagcc atcgccacgt cttgcgcgcg gcggcggtgt acgtcggcgc ggtgtgggcg
      181 ctttcgcagg gcattgccca acttgcgccg gtcttcgatg cgccgacctg gatcacgcgc
      241 tggttcgtga tcgcctgcgc ggtcggcttc ccgttctggc ttgcgttcgc ctggtactac
      301 aagctcaccc cggaaggctt caagcgcgaa accgacgtcg ctgtcgacga cccggcgttc
      361 caccgcgcca ccggccgcaa gctggacttc tggatcatcg gcgtgatggc ggtagcgatc
      421 gtgctgctgg tcaccaatac cttcgtgctg caccgcgacg tcaacagtgc ggcgagtgca
      481 gccgacgccg agacgatcgc ggcggaactg gccaaggtcc ccttccagtc cgtcgccgtg
      541 ctgccgctgg cgaaccgcgg tgattccaag cagcaatact tttccgatgg gctctccgag
      601 gaattgattt ccgacctcac ccaggtgaat gggctgaagg tcatcggcag ctactcgagc
      661 ttcaagttcc gcgacagcgc ggacagcccg gcgcagatcg gcgccgcgct cggcgtggcc
      721 cacctgatac tgggatcggt ccagcagcag ggcaaccgca tccgcgtcac cgtgaacatg
      781 atccgcgcga aggacggagc cagcgtgtgg tcgaacagct atgacgagca gctcgaggac
      841 gtgttcgcga tccagtcccg gatcggcgac gcagtggcca aggcgctcaa gatcaagctg
      901 ctgggtaaca cgatcgtctc cagcgacagg ccgcccagcg gcagcgtcga ggcatacaag
      961 ctcatgttgc agggacacgg cctcgtacag caatacacgg tgtccggctt caaccagggc
     1021 atctcgttgc tcgagcaggc gctcaagatc gaccccgatt acgcctatgc ctggggcgta
     1081 ctgtcgaccg ccctgatcaa ccagggccag acggtgctgt cgggcgaagc acgccttcag
     1141 gcgtacgcga aagcgcgcat cgcggcggac aagcagcagg agttggcgcc tgacgcagca
     1201 gatacacacc tgacccgcgg ctacctgctc cagaacgtcg acctcgatcc catgggcgcg
     1261 ttggcagagt tccgccgggg cgtggagctt gcaccgaacg acgggagggc catggagttc
     1321 ctggcctacg gcctggccag tgcggggcaa ctgcgggcgg cagtggagtt gttccgcaag
     1381 gccatcgata ctaatccctt caatacgccc tggtacgcga cattggcttc catcctgttg
     1441 ggtaacggag aactcgatgc cgcggagcag gcgagccgca agacgctcga gcttctaccc
     1501 ggaaccccgg ggatgtacgc gaacctggcg ctgatcaaca ttctgcgcgg cgatgccgcc
     1561 gcggccgcgc gcgatgcggc acaggagccc gaccctgtac tcggagcctg ggcccgtgca
     1621 atggcgctgc agattgatcc ctcccgtgcc aaggccgatg cggcgctccg cgattacgtc
     1681 gcagcacacg gcgagagcga gccttatttc gtcgcggacc tgtacgcggt gcgaaagcaa
     1741 cccgacgaga tgttccagtg gctggagcgc gcattcggtc agcatgatcc ggcttttttc
     1801 agcctgctct acgatcccct tccgctcgcc tacaagaacg atccgcgctt tgccgccttg
     1861 tgcaaacggg cggggctgcc ggttcccggg caaccgctgc ccaccatggc aggcgtgacc
     1921 aaccagtaga aggtcagggg gccggtgccg ttggtcgaga cgtgcggcgg agagcctgtg
     1981 gcggccgcgc gtttcgggca agcggaagga ctgccggccg gtccacggcg tcggcaaagc
     2041 ttgcgggtgg tcggcatccg gcgcacaccg gttgagtggg ttgcggtgcc gccacccggc
     2101 acgacggcca aaaaagatcc ccgtttccgg ggatctttgt atgtggagtt gaatcgcggc
     2161 ttgcgatcag gacgttcgcc gccggcgcaa ccccgcgaac aggccgagca gcagcgtccc
     2221 caggccgaac atcccgagca ccgaaggctc cggcacggat tccggattca aatccagcac
     2281 caggccgaac tcgtccccgg cgccgaggta gaagttccgg ccgcagttcc ggccgcagtc
     2341 gaattgcggc tggatccaga tggcgtcgat gtcgaggctc tccagatacg tctcgctata
     2401 cgtgcaggag acgatgccgt ttgcgtccgg gccggagcag gttggctcaa gcgccgtgag
     2461 cgtcggcgtg cagttgggat ccagcagcga acaggaggct ccccaaagtc cggcaaacgg
     2521 atcctgctcg accgagagca gcgtgatcgt cacggaattc caattgtgga attcggtgat
     2581 gttcaattcg atcatctgct gcggcagtcg atcgatttca ttggtctcgc ccgggaccgc
     2641 acacgaattg gcgccgccgg gctggcttcc gcagacgccc aggcccggat tgcccaaggc
     2701 atcctggcca ttggtcatgt agacgccgta ccagccgttg ccatcatcct cccaggcgct
     2761 gagggtcacg aagctgggat cctcgatggg acccgacgtc cacacctggg agccaccggc
     2821 ggcctgtatg cccacatgga tcggtacggc cttggcgccg accgagaaca gcagcgcacc
     2881 ggaggcaagc gccgcggaaa cgagcaggga attgcgcatg gccagcctcg gaaaattcaa
     2941 ggaaaaggtc tgcggcccgg gcggccttcg gcagttgcaa ggccgggacg acgttccgca
     3001 atctggcacc tgccaagcat ggggcgtgcc gaaaccgcaa gttattgaaa tccaaaagct
     3061 gtttccgcat cgtctgctcc ggatttgcgg gatttgtaag atcttccgac agctgccggc
     3121 cgcaagcggg gtcggttttg gggctgcggg cggcaccacg gctgccatcc gacgcgtttt
     3181 cggatatgta agatgcttcg acggagagcg cggctggaag agcgcgcctg ccccgcaagc
     3241 cctgttcgcg atcgcgagcg gaatgcgctg gtgtgcctgc cacctcgcaa tttccagccg
     3301 ccggccagcc gttgatggac ttcctgctcg agcaccacat cgatcttccg catatctgcc
     3361 aactgcagcc gatggggccg atccagtccc gcgactcctg ctgggtcgag gtgggcggtg
     3421 aactgaagcg ggcctgcgcg cgcaagagcg ccgagggaat ggagatcgtc agcgccagcg
     3481 agcgcgccac gaggacatgg accgcatcct cgccaagcac gagctgtact gcaccgtctg
     3541 cgagaacaac accggcgact gccagttgca caacaccttc gccggtatgc acatgccgat
     3601 ccagcgttat ccgctcaccc gcaagccctt ctacacctac gatcccgacc agtgcatcct
     3661 gtgcggacga tgcgtcgtga aggcatgtca ctcccccacg agcatcaaca aattgaggga
     3721 atcgggatgg tggagctgag gaggatcgaa ctcctgacct ctgcattgcg aacgcagcgc
     3781 tctcccagct gagctacagc cccacatcga attttcttcg gcggtcggcc cggatgaggg
     3841 caccgccacg gtggcgcatt gcacgccagc gagccacgta tggtagccgg tatgcggccg
     3901 ccagccaagc cttgcccgaa gaaaaaccgg gaaaaaccgg ggtcagagtg ccctttcggg
     3961 caaaagatcg cgcacatttg attcggcgcg cgaaagggca ctctgacccc ggtttttctt
     4021 ccccggtttt acggccttca gtcgggcagg agcggcgtca atacctcctc cagcaagcca
     4081 cgccgccagc cctccatgcc ctccggccac tcccgcgtcg tcgcgaattc gacgaccagg
     4141 cggcgcgggc agagcagccc cggcggcagt tcgagtcgcg cggcgacggc ttgcaccgcc
     4201 atgcgcatcg catcgatcac ctttttgtac tcgccacgcg gcgccggcgg gatgggggac
     4261 agctccgaca actccccggc cgtggccggt gcctgcagca gcgcaaacaa ctcggtgcgt
     4321 tgcgggccgc gcagggcgcg ctgccccttg acccgatcga ataacgcctg gggcgtggcc
     4381 ggcggttgtt gcgccagctg cagcgcatgg gcgtcgtcca gcagccatgg ccgcggcttg
     4441 tccagggtgc gggcggcttc gtcgcgccac agcaggatcc ggcgcagccg tgcctgcact
     4501 tcggggggcc agtcggaagc ggccggaaag cctttttgcg gctgtgggtc ggtgccgcgg
     4561 cggcggtccg cccgccgcac cagccgctcg caatcctcgg catgccattc tcggcgaccc
     4621 agccgcgcaa gttcgtcgga gagcttgccg tgcagctcgg ccaggtaggc cacgtccagt
     4681 tcggcataat cgcgctggct ggcggtgagc ggccgctgca gccaatcgga gcgcgtttcg
     4741 tccttgctga tctcgatgcc gagcagtccg gccaccagct tctggtaact caaccccggc
     4801 cccattccgc acaaggccgc cgcgatctgg gtatcgaaca accggagcgg cacgtcgacc
     4861 agcatcggcg cgagcgcctc catgtcttcg ccggcgctgt gcatgatgca gcagcggccg
     4921 gtgacgagcg accgcacgtc cggcccggcg tcgtaggcca gcgggtccag cagccagtgc
     4981 tcggcgcggt gtgccgcctg caccagggca agcttgggga aataagtgtt gcggcgcatg
     5041 aactccgtat cgagtcccac ggggtcctgg tcgtcgagcg tcgccaggca ccctggctgg
     5101 tcgatccagg gtgccgtcat cgcctgctac cgtgcatgat tcggtgttcc attgcgcacc
     5161 gctttgctat cgtgaaccgc acacgataac agccggactc cgcacccgca tgccgaaccc
     5221 gcaattcatc acccggcaac acgcgatcgg cggcacgatc ggtgtgttgc tgctggtgtt
     5281 cgcgctggtg tatcgctttt ttccggcggc cctgaaggtc gagcccgcgg cgctgcggtc
     5341 gcatccggcc acggcgagca gcgcgccgct ggtggctcgc cagccgccac ggaaactgac
     5401 ccccgcggaa tccgaactca acgccggtcc cccgatttca ctggcgccga ccaaggtgat
     5461 cgccgcccgg gcacgcgaag gtacgctggg ggcacaagtc gggacaggcg aggtcgcgca
     5521 actgcttggg cgcgccgggg cagcggccgc gagcggcaac ctcgcggggc cgggcgagga
     5581 taccgccatc gcgctggtcc tgcaggcggt gcaggaagcg cccgacgatg cccacgtcaa
     5641 gaaggcggtg tcactgctgc atgcgcgcct ggtcgcggac gcgcaacaat cgctcgcggc
     5701 cggcgacgcc gaggcggcgc gaaccaacct cgatgcgctc aagcgcttgc ccggagccgg
     5761 tgccgacgtt gcggcgctgg cggaccggat cgacgccgcg ggcaaggtcc agccgttgct
     5821 cgagcacgcg tcgaacctgg tgcagcaggg caagctcgaa ggttcgggcg acgacaccgc
     5881 gctcgcggtg taccgcaagg tcctggcgct cgatccggaa aacgtcgtcg cccgccaggg
     5941 gcttcagcgc gtgcagcagg cgtcgctcga ccgcgccttg acggccgccg cgcagagcga
     6001 ggacgccaaa gccgatgccg cgctggcgga cgcggcggcg atcctgccat cctcgcgcgc
     6061 gctggcagac acccgcgccc gcatcgcgac gattcgctcg ggccgcgccg acaacctgat
     6121 ggcacaggcc aaatccgcgc tcgatgccgg caatgtcgac ctggcccaac ggctcgccga
     6181 ccaggctgcc gggctgggtg cctcggccga cgagttcctg gcgcgattga agaacgcgcg
     6241 cgagtacgcc agctaccatc ccggccaggt cttttcggac catttcctgg acatcgtcgg
     6301 ccagtcaccc gcgatggtcg tgatcccgag gggcagtttc ctcatgggcg ccgcctccgg
     6361 cagtggcgcc agtgccgagc agccccagca cgaagtgacc ctcgcgaccg gcttcgcgat
     6421 ggcgcgcacg gaggtgacgg tcgccgagtt ccgtgccttc gtgcgctcct cggggtacgt
     6481 gcccgactcg aagcgtctgg ggggctcgtc ggtgtacgac gagaccaacg gccgcatgca
     6541 gcagcgtgcc ggcgccgact ggcaggacga ttacgccggc aagcgtgcca agggttcgga
     6601 tccggtggtg aacgtttcct ggaacgacgc acacgcctac gctgcgtggc tgtcccggca
     6661 caccggcaag caataccggc tgcccaccga ggccgaatac gaatacgcgg agcgggccgg
     6721 cagcacgacc ctgtactggt ggggcgacgt cacgccgaag gtcttggtgg agaacctcac
     6781 gggcagcggt gaccggtcgc ctgcgcaccg gagctgggcc aatgccttca agggctatga
     6841 agacggctat tggggtccgg cgccggtgat gagcttcctg gcgaatccgt tcggcttgta
     6901 cgacatgagc ggcaacgtgt ccgagtgggt cgccgattgt tggcacgaaa actacgtccg
     6961 cgctcccaac gacggcagcg cctgggtcaa ccccggctgc agcgaacggg tgatccgcgg
     7021 cggctcgtgg ggcagcgcgc ccgaccagga ccgctcctcg ttccggcagg gcgcgcccgc
     7081 ggaggcgcgc agcggacgtg tcggcttccg cgtggtgcgg aatttgtgat ggaggcttct
     7141 cagcggcagg cggagagcgc cgaccgcagt gagcccaggt agccggcgcc gaacaggttc
     7201 acgtggttca acaggtggta caggttgtag acggacgtgc gctcgcgcca atcgggccgc
     7261 agcggtgcac cttcggcgta ggcgtggaag aacgcgtcgg gcggcgatcc gaacagcgtc
     7321 agcatcgcca gttccgcctc ggcccagccg taatgcaccg cgcctgcgtc tatcagtgca
     7381 ggcgatccgt cgccacacac gtgcaggttt cccagccaca ggtcgccgtg caacagtacc
     7441 gccggcaggt cgggtacgag ttcgcgaagc cgtctgcaaa ccgattcgat cgcagcagca
     7501 tcgcggccct cgatcagccc gcggtcgcgg gcccggcgca tctgcggcag caggcggcgt
     7561 tcggcaaaaa aacgccagcc atcgtcgtcc ggcgtgttgt cctgcgacga atcgccgcag
     7621 aatccgccgt gggtaaggcc gaaccgttcg ccgcggcggg cgtgctgccg gccgaggccc
     7681 gagccggccc gaagccagta atcggcggct ggcgtgcctt ggccgagatc ttcgagggtg
     7741 atgcggtcgg cgtcgacctc gagcacgtcc ggcacgcgga gcccgcctgc atcgcgcagc
     7801 aaacccaggc cgcgtgcctc cgcctcgaag aacctcgccg gcgctcccgc gcggtgcttg
     7861 gtgatcgtgc cgcccccgcc gcgctggatc atggctggct tgccaactgc ggggcaccct
     7921 cgtggctcga tcgggaatgc aggcatggct gcgcaaagcc cacgtggtgt cgcagcgcgc
     7981 acgatattcc cgtgcacttc ggcacgaacg tcagtggcgg cgctgccaca tgcacacgcg
     8041 cttgtcgccc tggtcggaag aaacaacctt gcagaccttc ttctcggcgt gcgggtccgg
     8101 gaagtcggcc ggaaccgtgt cgtgcgacga ctgcggagat ggagcatggt agggagtcgg
     8161 gccaggagag ggaatgcggg gccgcggcgc aggcacggtc gcgcacgcgc tgcaggcagc
     8221 gcaaagcgcg accaatgaca tgactttcga agccttgcgc atcacgcacc atctgcttca
     8281 cgagccaacg tccgcagcgt aacacttgcc gggacccgcg gtgcatggtg ccgaaggtgg
     8341 gactcgaacc cacacgcttt taagggcggc ggattttgag tccgctgcgt ctaccgattc
     8401 cgccacttcg gcaaggggcc gcagtatgag gcagcacgtt gccgatgcca agcattgccg
     8461 ggcccctgcg tcccgcgatg aacatgcggc aaccggtggc gacgaattgc acgatcgcgc
     8521 ggctcgtttc ccggcaattc atgggtggtt tcgcatcatg gctgggcgat ggaacgcgcg
     8581 agcggcccat tgcgccacgc aatcatggac aaggagtgtt gcatgcgatc cctcaaactt
     8641 gttttcttcg ggcttgccgc ggtcgggatg tccgccggcg ccctcgcgca gaacgcgccc
     8701 acccagaatc cgccgaccca gccggcgacg caggccggga gcaagcccgc tggccagctc
     8761 ggcaactccg gcggcggcct gagcatgggc atgacgccgc gcgaagcgca aatggcgttc
     8821 gggaacatgg atgccaacca cgacggcttc ctcgaccgtt ccgaattcat gcacaacggc
     8881 gacagcggcc agcgtttcgc cggctgcgac gtgaaccgcg acggcaagct ttccgaagcc
     8941 gaatacgtgc agtgttcgca gcgaccggca acgcaggacc agggaaacgg acaaccctga
     9001 cgtcttctgg cgctggccgt ttcagtccag ggtcaggctg cgttcgccgc gcgcatacca
     9061 ttcgccgccg atcggctcga acaccgagca gtgcgtcatg cggttctggt agacgtgcac
     9121 gctgatcgcg agttcgcgat cgctggcatt gcagatggtg tggtgctcgt gcggaggaat
     9181 caggctgccc gccgagcctg tgccggcacg gatcgcgcca gcggccacga agcgatagcg
     9241 tgcctcgtta tggtcgcgca gttcgtactg caccacgtcc agcgctccct gccacacgcc
     9301 ttcgacgcac cacatgccgg catggtcgtg gatgggtgtg ccctgcccgg gactccaggt
     9361 catcgcgacc acgcagtagc cgtgctcggg gctgcaatag agctcgcggc gcgcatagtg
     9421 gccatcgcag ccctcgaata cgaagccggg caggtgtacc tcgccggaat ggatcgcatc
     9481 gcacagcgcc gagcgcagtc cgcgcacggt ggccgtggtg tccggcgcct gcacggcggc
     9541 gtcgaacgct cggatcatcc ggtcgcggcc gggaaagtcg atgcccatgg cccaagctta
     9601 tatcggtccc gcacgcgggt gaaagccggg gtgcctaaag ggtggccatg aatgcgccga
     9661 tcgcggcgcc gaagcggccg gtgtcgacca ggaatgcgtc gtgtccttgc ggtgaatcca
     9721 ggccgacgaa cgtggtgtcg gtaccggtgg cggtgaggtc ttcggcgagt tgctgctgct
     9781 gttccagtgg aaacaggatg tcggtttcga cgccgatcac cagcgcgcgc tgcagcttca
     9841 ttttcgcgag ggcgttgcgc accgcggtgg acgaatcggg ggcgccgccg tactcggcca
     9901 ggtcgaacca gtccgaggcg cgcgacaggt acaggtagca gttcgggtcg aacgaacggg
     9961 tgaaccggcg cgcatgcgcg ttgagatacg actcgatctg gaattcggcg ccgaaggggt
    10021 cgtcctcgcg gtcgccgggg tcgagccgga tccggccgaa gcgtccgcgc cattccagcg
    10081 acgagcggta ggtgatgacg ccgagcttgc gcgcgatgtt catgcccgtt tctgggtagt
    10141 ggtcgtcgtc gtaacggcct tcgttccagt tcggatcgag ccgtatcgcc tcgcgctgca
    10201 gcgagcggat cgcgatcgcg aagggttgcg cgcggggcgc ggtgcatacg ctgacgtggg
    10261 cgcgcgcgaa gcccgggtgg tgcgccaggg aaccgagcgc cgacatgccg cccatggagc
    10321 agccgaccag tgccgcgagc cgctctatgc ccagcccgtg cgccagttcg tatccggcgt
    10381 cgccgatgtc ttccagcgac aagtcgggaa aatcgaggcg ccagggttcc ccggtgacgg
    10441 gattgggcga tgcgggtccg ctcgagccct tgcaactgcc cagcgagttc atgcagatga
    10501 cgaaccagcg gttgctgtcg atcgccttgc cgggccccac gatgtcttcc caccagccgg
    10561 gcgagtcgtc gatcgcgttg tgcgccgcgt gcgcgcttgg tgacagaccg gtcaggatca
    10621 ggaccgcgtt gtcgcgcgcc gcgttcaacg tgccccaggc ttcgtaggcc acttgtccgc
    10681 cgcgcagttc accgccgcgc ttcatcttga acggcgaggg cagggtgaag tattggcggg
    10741 cgtcggccat gggcaatcgt cggcaggcag aatccgcaag ggtagcgaac gcgcgtggac
    10801 gggccaagcg acgcacgatt ccttccccct tccataggaa ggagatcgag acggatggct
    10861 ttggctggtg gcgcatcccc cggcgctgac gcgccgaccc ccttcctttg gaagggagtt
    10921 actgcactcg ctttgacaag tcccgggtca atccacccgg tcgatcgtca acgcgaccgg
    10981 tgcgtgcgac ccggtggcga tctccaccga tgccgattcc aggtcccccg cctgcggcat
    11041 cgcgttgccg gacttgctga tgcgggcggc cacctgcact ttcgggaact tcgacagcgt
    11101 cagcgcgggt gtcatcgcca ttgcatcggt caacgtgacc ttggccggca actgcgaggc
    11161 cttgaggcgc gcgaccgcca gcggcatggg tggcccgtcg accgcgcgcg cgaacacgaa
    11221 caaggtatcg ccgggctgca ctttcgaggc gaggtcgggg gcgagcttca cggaaaccgt
    11281 gagcgcgatc tctccggcgg ccgctcctga tgaaccggca ggtgccggat cggctgccgc
    11341 aagctcgccc gtccggacgc cgccacccgc gcgtgcctcg gctgccgcca attcctgttg
    11401 tacggcgtcg gcgaccttcg agcccggttg cagcagcggc agcaaggtcc tccatcgggt
    11461 ggccgcgtcg tcatactggc cgcgctggaa atcgctgatg ccgagcagcc acaacgctcg
    11521 ctggtgaccg gggtcgatct gcagggcgcg ctgcaactgg tcgcgcgaag cgtcgtcgat
    11581 ccggtgcgac ggactggttt cggccctggc ctcggcccat gcgaccattg catcgggatc
    11641 gtcgggcttc agtttcagca agtggtccaa tgcatccagt gcctgttgcg gctggcccat
    11701 cgcggcgtac gcctgcgcaa gcagcgccca gccctgcgcg tctccgggct ttttcgcaag
    11761 gctcgcctgc agttgctcgg tggccgcggc aagatcggca cggttgtcag gcacggccgg
    11821 ttgcagtgcc tgcggcgttc cgatcgcgag gtacatcgcc agcacggctg gcggcacggc
    11881 cagggccagc aggagggcga gtacgaaggg tgtgcgcggg cgaccttcgc ggcgggcgct
    11941 acgcagcatc ggcaccagca cgcaggcaag ggcgaccacc accatggcgg cggcgatgat
    12001 gagaaaacgc gtggtcacca atcgtctccg gggtcttccg cgatgctgcc ggccgggcgc
    12061 gtgcgccggc gcagcagcac gaccaccacg atgccgcctg cgagcaggat tgcaggcgtg
    12121 ccgaaccaca gcgcgatggt gctgggggcg agcggcgggt cgtacaggat gaaggtggaa
    12181 tagcgcgcga ccagccaatc cttgatttcc tcgtcgctct tgccctgctg catcatgtcg
    12241 aagatctgcc gccgcaactg cttggcgaag tcggcgttcg atgccagcag gctttcgtcc
    12301 tggcagacca tgcagcgcag ttcgccggtc aggtgctgga agcgttcgcg ctgggcggtg
    12361 ctctggaacg gcagcggatc gatcgcgaat gcgccggcgc tgaacgcgag cgcgaatgca
    12421 acgacaacga cggccatcgg ttgccgggtg cgtcgaagcc aattttttcc ctctcccttc
    12481 gggagagggt gccgcgcagc ggctggtgag ggtacggaat ccgcgcaatg tctcgctacg
    12541 cgcgtaccct cacccgcccc ttcggggcac cctttcccgg ggggagaggg aaggagcatg
    12601 gccagcaggc gcttcatgtc ccctggtcct ccagttcgcg gatccggggt tcgagctccg
    12661 tggcgatcac gtcgggcgtc agtggcccga tgtgcttgta acggatgatg cccttggcat
    12721 cgatcaggta ggtttcgggc gcgccgtaca cgccgaagtc gatggccgta tcgccggact
    12781 ggtcggcgat cacctcgtgg tagggattgc cgtgccgctc cagccaggca cgcgcatccc
    12841 ggggcgcgtc cttgtagtcg tagccgatca gcggaatgcc gaacctgtcg cggtaggcca
    12901 tcagcaccgg gtgctcgtca ccgcaggcga agcaccagct cgcgaacacg ttgatgaggt
    12961 acggcttgcc gaggagatcg gccttgctga cgaactgggt cggctgctcg agcttcggca
    13021 accggaacgc gggcgccggc ttgtcgatca atgggctcgg cacgaagcgc gcgtcgtgcg
    13081 tctggttcca caccaggccc accacgaaga acgccaccag cgccgcgaac acgatcagcg
    13141 gcacgaagcg gcctatgggt cgccgtgcaa cctcgttcat gcttcgcctt ccgccagctt
    13201 gggtggcacg acgggttcgg gcgtggcgcg caccgtttcg ggttcgaccg cacgacgcag
    13261 gcggaagcgc ttgtccagca ccaccgtgaa gccacccagc atcatcagca cgccgccgcc
    13321 ccagatccag cgcacgaacg gcttgtcgta cacgcgcaac gaccacgcgc cgttcccgat
    13381 cgaatcacct agcgacacgt acaggtcgcg gaacaccccg gcctcgatcg cggcctcggt
    13441 ctggacctgc ccgccggcat aggtgcgctt ctgcggatgc agcgtgacca cgtgcctgcc
    13501 gttgcgggtg atcacgacgg tgccctgctg cgcgcggtag ttcggacctt cggtttccac
    13561 gacgccattg aagtggaagt cataggcccc gacttcctcg cttgctccgg gcttcatgct
    13621 gacgtcctgc tcgacgctca tcgaacgcgt caagatggcg cccgcgatga acacgccgat
    13681 gccgaagtgc gccagcatca tgccgagcat ttcgaacggg tagcgcttgc cgcgcggcgc
    13741 ctcgcgccat cgcttgatcg cgaaaagagc gacacccgcc atcacccaca cggcggcggt
    13801 gatgccgagc agttcgtaga agctgcgcgc gccgagtgcg aagccgatga tgccggccgc
    13861 gcccgcgagc gcgacgatcc ggatcagcgc gttgcgccac accttgaggt cggccttgcc
    13921 ccagcgcgac aacggcccga acggcagcgc cagcaccagc ggcgtcatca gcagcaggaa
    13981 cagcggaccg aagtagggtg gcccgacgga gatgcggccg atgtccagcg cttcgccgac
    14041 cagcggatac agcgtgccca gcagcaccat ggcgcaggcg acggtgagca tcaggttccc
    14101 gatcaccatc atggtttcgc gcgaagccag cgcgaacgat ttgccgccgg tgaccttggg
    14161 cgcgcgcaat gcgtacagca gcaacgagcc gccgacgacg accgcgaggt agacgaggat
    14221 gaacacgccg cgcgccggcg cgaaggcgaa cgcgtgcacg ctggtgagca atccggagcg
    14281 caccaggaac gtgccgagca gtgaaaacgc gaatccgaag atcgacagca gcagcgtcca
    14341 tgcacgcagg ccgtcgcgct tgtcggtgat cgcctgcgcg tggatcagcg cggcgccgat
    14401 caaccacggc atgaacgagg cgttctcgac cggatcccag aaccaccagc cgccccagcc
    14461 gagttcgtaa taggcccatg cgctgccggc gacgatgccg agggtcagga atccccatgc
    14521 gacgttcgtc caagggcgtg cccagcgcac ccaggtggat ttcagttcac cgcccagaag
    14581 tgatgcgatc gcgaacgaga aggcgacgct gaaaccgaca taccccatgt aaagcatggg
    14641 cggatggaac acgaggcccg gatcctgaag taccgggttc aggtccgcac cgttcggcgg
    14701 caccgggaac agccgcagga acgggttgga cgtcgtgatc atgtacagca ggaacccgaa
    14761 ggcgacgaac ccgagcacgc cgatcacgcg cgagaagaat gcctttggca gccttccgcc
    14821 gagcgcgacc atcgccaccg tccagaggtt caggatcagc atccacaaca gcagcgagcc
    14881 ttcgtgcgcg ccccacacgc cggacaactt gtagtaccag ggcagcgaga ggttcgagtt
    14941 ctgggcgacg taggcaaccg agaagtcgtt ggtccagaac gcccacgcga ggatcccgaa
    15001 cgccagcgcc acgaacaccg cctgaccgat cgcgaggctc ggtgcggttg ccatcaacgg
    15061 cttgctgccg cgccatgcgc cgacgatcgg cagcaggcac tgcaggccgc tcagcagcag
    15121 cgcgaggatc agcgcgacct ggccaagttc ggggttcatg acggcaggga ccggggaccg
    15181 ggggccgggg accgggaaca gcggattcgg tcgcgtactt tcggattact catcaggtca
    15241 ctccgttccg tgcggaccgt ccgagtcgcc ggcagcggat cggtgttgtc cggttcccgg
    15301 tccccggtcc ccggtcccgc gctccgggat ccgatgctct tccttcgcct tcgcgatcgc
    15361 ctgctcgacc tgcggcggca tgtaggtcgc gtcgtgcttg gcgagcacct tggtcgccac
    15421 gaacttgccg tcgaccatgg tgccggtggt gatggtgctc tggccgaccc ggaacaggtc
    15481 gggaaggatg cccgtgtagc tcaccgggat gctctggaag cggtcggtga tcacgaagtc
    15541 ggtggtgagc gtgtccggca cgcggtggac gctgccttcc tcgacgatgc cgccgaggcg
    15601 gaaggggtgc cccgccggcg ccttgccggc gtcgacttcg ctcggcgagt acaggtaggt
    15661 cacgtttcgc tgcagcgcga acaccgacag ggtggtcgcg atgcctacgg cgccgaggat
    15721 cagcagggca acgatcaaac gtcgcttgcg gaccgggttc atgtcgatgc ctccgcgcgc
    15781 gccgcgcgcg tgttgcggat acgctcgcgc gcaatccgtc cgcgcagttc gcgcgtcagg
    15841 cgtcggcggc gcagcgcggg ggcgaaccag tcccacgcca gcaccgcgaa gaacaccgcg
    15901 taggcgggcc acacataaac ggcgtacccg cccattgcaa gccagtgagc catgtcaggt
    15961 aggtgccccc gtcgtctgtg cgggttgtcc gtcgaccagt tcgcgcaccc actgcttgcc
    16021 gccctcgagg gagagcaggc cggtgcgggt gcggcccagg atgctggcga agaagtacag
    16081 atgcgtggcg agtgccatca ccagcagcgg ccacagcatc gcgggcgcga tcgaagactg
    16141 gccgaggttc atccagtcga ccgtcgagcc ctggtgcagc gtgttccacc attgcaccga
    16201 gtaatgcacg atcggcaggt tcacgatgcc gaccagcgcc agcagcgagg cggcgcgcgc
    16261 gccgcggcgg cgatcctcga aggcgtggta gaggccgatc acgccgaggt acaggaagaa
    16321 cagcacgagt tccgaggtca ggcgcgcgtc ccaggtccac caggtgcccc acattggctt
    16381 gccccacaac gatccggtgg cgagggtgac cagggtgaac gcggcgccgg ccggggcgca
    16441 ctccatggcg atcacttcgg cgagcttcac ccgccagacc agcgcgacca ggcccgccac
    16501 cgccatgcac gcatagacga acaggctcat ccatgcgctc ggcacgtgga tgaagatgat
    16561 ccggtaggcg tcgccctgct ggtaatccgg cggagccagc accagcccgc cgtacagccc
    16621 tatcgccgcg gccacaagcg ccgccgccat cagccagggg cgcaacgcgc cggcgaaccg
    16681 gtagaaggtc ggcggcgatc cgagccggtg catccagtgg gggaggtaac tgcgggacat
    16741 agtctgcaaa acacctgtca gccggcgaat gtgccaccgt cgcgagcatt cttccgcggc
    16801 ggccgatgcc gaggcacccc gcggcgatcg tgcgctcgac gcgccagcgg gccgcgcgcc
    16861 ggcgatccgc gctccggacg cgccggaccg cctttccatg ttggtgttgc cggaacggtc
    16921 gtcatcacgc ttcaaccgcg atccgcaggg ccgccgcgca cgcgagcggc gccagcacga
    16981 tcgccaatac cagcgccgca gccagccacg acagcggggc caggtacggc atgccggact
    17041 gcaccgccgc gatggcgccc gcagcgaaga tcaccaccgg gaccgccaga ggcagcagca
    17101 tcaatgcgag aaggatacca gagccgcgcg ccccggccgc cagtgccgac agcacggcgc
    17161 ccagcagtgc cagcagcggc gttgccagca acagcgccag catcaggacc ggcaggacgt
    17221 cggcgggcag atcgagcagg gctcccagca gcggcatcac gatcagcaag ggtaggaccg
    17281 tggtgagcca gtgcgcgatg acccgggtca agaccagcag tgccagcggc tgcggcgcca
    17341 gcagccattg ctcgagggtt ccgtcatcga ggtcctggcg gaacatcgat tccagcgccg
    17401 gcagcatcgc cagcaccacg atcaccagca gcacgcctcc cgcgatgcgg cccagcagtt
    17461 gcgggtcggg accgagcgcg aaggggaaca gcgcggtcac gatcacggcg tacagcagcg
    17521 gcaggaacag ttcgccccgg cgccgccagg ccagcaggaa atcccggcgc agcagggcac
    17581 cgcaggcgtg ccacgtgccg gccgggcgcg gccgcgccgc cggggcgacg ggcctttcat
    17641 cggtgacggg ctggacggcg gtaatcatgg ttcaagcggc cagccggatg tgccgcgcat
    17701 tcggatcggc gaagcgcacc gcgccgtgac tggtgacgag tgccgcgcca ccgccagcgc
    17761 agtgctcggc aatcagcgca ttgaccagct cgatgccgtg ccgatcgagg ttggcgtagg
    17821 gttcgtcgag cagccacagg tcgcctggca gcagcagcaa ccgggcgaga cccacgcgtt
    17881 tcttctggcc ggccgaaagg ttgcgcagcg gttcgtcgat ccagccttcg atgccgaccg
    17941 ccgccagtgc ggattcgacc gtcgctccgg cgtgcgtgcc gtacagtccc gtcaggaacg
    18001 cgaggttctc gcgcgcgctg agtgaatcgt tcatgcccag ccggtgcccc agcagcagaa
    18061 cccggccgtt gcggctgtcg cgggaggcgg gcgtgccgtc gaagcggatt tcgccttcgc
    18121 ccggaagcat gcctgcgagg acccgcagca gcgtggtctt gccggatcca ttgtcgcctt
    18181 cgaccagcaa cgtttcgccg cggtcgacat tgaaatccag ggggccgaac acgggctcgt
    18241 cgttgcgcgc gaaggcaacg cgatgcgccg ccagcagcgg cacggcgggg gaggcggaag
    18301 ggctcatgat tgcaaccgcg tattgtcgtg gacgccgctg gcagtgtcca tgacccggcg
    18361 gttttccggt atcggcggga ggcggatcgt agcgtgggcg ggggccgggg tgcgcccggt
    18421 ttcagaccag tacccgcagt tcggcgatgc ccgtgccggt gccgcggacg atgccgagct
    18481 ggccgaaggc gcggcccagc gaatcgagtt gctcgaaggc gacgccggga acgaacaggc
    18541 tgggctcgcg ccagtcgttg ggacccacgc cgatgcctat aaagtagcga tggccctcgt
    18601 ccctgagttg gtcgcgcagg cgacgctggc ggatacgatt gtcggcggcc ggcaggctga
    18661 ccccggccgg attccaggcc gtgatgtagc cccaggggtc cccggcgcgg cccagcagtg
    18721 ccagcagggg agcaggcagc ggttcgccac agcggatgac cacgtagccg ccccatgtgg
    18781 tacgcacgcg atactcggtt tcggtccatg cgcgatggat cgattcgggc aagggcatac
    18841 ggtcagccgg aagacgccag gagcggcagg tctgcgcgtg cgcgtgccgc agccgcgtcg
    18901 agatcgatcc cctcgatgct ttcgtgcccc gccaggatcg tttccatcaa cgccgtctgt
    18961 tgccggtcgc gcgcaacgca ggtcgcgtag ggtagcgtcg tgaagctcac cattctgtaa
    19021 cgggacatga accggtccgg cacccgccgg gtgagggccg cggcgagctc gcgcttcaag
    19081 aggtagtgcg gatcggcgac gccgtcgcgc atctcgacgt agttggcgag cgacatggcg
    19141 gcgatcgcgt cggcattcgg cttgcgccgc gcttcgaacg ccgcaaacat gccgccggga
    19201 tcccccggtt gcgccgcgag caggtccgcc agtgcggcgg cgtcctcgaa gccggcgttc
    19261 atcccctggc cgtggaaggg cacgatcgcg tgcgccgcgt cacccagcag cagcgcacgg
    19321 ccgtccagat gccagcggtc caggtacagc gttgccagtt gcccgatcgg atgatctgcc
    19381 cagtcggcat cgaaggccgg catcaatgcg agcagatccg cgaactgtcc ctcgaacaac
    19441 cgccgcgccg ccgcgatgtc cggaagggtc gcgaagctcg ggaacacacc ctcgctcggc
    19501 aggaacagcg tgacggtgaa gttgccctcg gtattgggca acgcgatgca catgtagttg
    19561 ccgcgcggcc agatgtgcag ggcgttgggc tcgatcgcga agccgggcgc cggggacgcc
    19621 gaatcgagcg gcgggatttc cagttccttg tagccgtgcc ccaacggttc gatccgctca
    19681 ccgaggtcgg tggttttcga catcgccacc cgcagtgccg aacccgcgcc gtcgcaaccc
    19741 agcagcaacc ccgcggcgtg ttgccgcggc gtgccatcca ggccggcaag cgtgatggtg
    19801 ttgcgctcga aatccacatc cgcaagcccg gcctcgaaat gcaatgtcgc gcccgccccc
    19861 tcggcggcat cgagcagcag gatattcaag ccgccccgcg ataccgaacg gatcacctcg
    19921 gagtcatcca gcccgtagcg ctgcaggttg gtcgaaccat ccggcgcgtg caccatccgc
    19981 ccgcgcatca tgacggcttg cgccagcacc gcatcggcaa gacccgcctg gcgcagggcg
    20041 tccaggccac gctccgccag cgcgaggttg atggagcgcc cgcccaggaa cccgtgagcc
    20101 cgtggatccg gccggcgttc gaacatctcg accgcgaagc cgcgctgcgc aagcagcgtc
    20161 gccagcagcg aaccgaccag gccagcgccg atgatggtgt acctggagct tgttgcggga
    20221 tgcgtcatgt ccacttcaac cggtcaaccg caaggtcgcc gccggtgtcc agcctgacat
    20281 cgagcttgcg catgagaaat gcattttcgc cgcgacgatg tccggccacg aacacgcggc
    20341 cgttcggctt tgcagggagg gaccggaaaa ccgcacccgc cggggattgg cccggattgc
    20401 cgccaacaac ttcgcttgcc cattcattcg cattggtgct gccatggtat tgcactgtcg
    20461 ggacctcgcc gtcgtcagcc tgggacgtgc caggcgctca cggcttccgc gtagcgcagg
    20521 cagtcggcga aggtgttgta gagcggcgtc ggcgcgatcc gcatcacgtc gggttcgcgc
    20581 cagtcgccga gcacgccttg ggcaaccagg tggccaaaca gcgattggcc ggcatcgcgg
    20641 cctgcgcgca cgcgcaccga cagctgcgcg ccacggcgat cgatctcgtc gggcgtcagg
    20701 atttccagca cgtcgccgag ttgcgtctcg atcagccagg cgaggtaggc ggtcagccgg
    20761 cgggacttcc ggcgcaggtt cgacatgccc gcgcgctgga agagttccag cgatacccgc
    20821 agtggcgcga gcgccaggat cggcgggttc gacaactgcc aggcgtcggc gccggccgcc
    20881 ggatcgaatt cggggcgcat ctcgaagcgg gtggcctcgt tgcggcccca ccagccctcg
    20941 aagcgcggca ggtgtgcgcc ggcgtgccgc gcgtgcacga acgcgcccgc cacggcgccc
    21001 ggccccgcgt tgaggtactt gtacgaacac cacgcggcaa agtcgcagcc gctgtcgtgc
    21061 aggtgcaggt cgatgttgcc ggcggcgtgc gcgcagtcga acccgacgat gcagccctgc
    21121 gcgcgcgcca gcctggtgat gacggcaagg tcgaatgcct gtccggtgag gtactgcacg
    21181 cccggcagca gcaccagtgc gatgcgcgag ccgtgttcgg ccagcgcgcg ttcgatggac
    21241 tgcatggaaa tcgcgccgcc gggctcgtcg ccatccagtt cgatcaatgc ctcgcgcggg
    21301 tcgtagccgt ggaagcggat ctgcgaggcg gcgaggtcgc ggtccgaagg aaaggcgtgg
    21361 cgttcgatca ggatcgcgtg gcgctgggcc gtcgggcgat agaaactcac cagcagcagg
    21421 tgcaggttca ccgtcagcga attcatcgcg acgacttcgc tgggttctgc accgacgagt
    21481 gttgccaggt tgtcgcgcac ctcccggtga tacggcagcc atgggtgtcg cccatggacg
    21541 tggccttcga cgcccagtcg tgcccaatcg tcgagttcat ccagcagcgc ctgccggacg
    21601 ccgcgtggct ggagtcccag cgaattgccg accaggtatg tctgcgtgcc gtcgccatgc
    21661 ggtgggatca ggaattcgtc gcgcaatccg tgcagcggat cggcctcatc ctggatgcgt
    21721 gcccatgcgt gatcagcgcg aaattcgtgg cttgcgatca tcaaggaaac ctttggcgtg
    21781 tcttgtcgcg atgctctgca ttaaaaccct ctccccccgg gagacgcggc cgtaggccgc
    21841 gcgtggcgct cccgcagggc gggtgagtga cacaaccgtc catggtgcaa acgccggggt
    21901 ggccatcctt ggccacccgt tcgaagcggc gcgtgatgcc actgtcatcg cgcaagcgcc
    21961 gcttctcacc ctcatccggc gctgcgcgca aaagcagcgg cggcgtgcgc ccttttcgcg
    22021 aagggggaat gccgcgctgc agggcgcctc agcgctccac ggtcgtcacg atcgactggc
    22081 acccggcatc gaaggtgttg cgccaatcga ccggcgcatt ctccggccgg atgcaacgca
    22141 cctggatcgc aagattggag cggtacgcgt agtgttcggt gtacgcgttc ttctcgcggt
    22201 tttccgtggc ggtatagcgc aggctggagg tgctacccgg catgttcact gcggcatagc
    22261 ccggaagctt gcgcgcacgc tcggtgccgg tgctgaggaa cgactggaag ccctgcaagc
    22321 cgtcgacctg ttcggtggtg acggtgatgc gggcgagcgc gttggggttc gccgggtcct
    22381 tgacctgcaa cgcgacgaac tggcgggcgc cgtcctgttt ttccatgagc acgggccatg
    22441 catcgggtgc cgagaacttc acggcgccgc cagccagcga ataatccgcg gcgtacgccg
    22501 gggcggccgc catcagcaat gcggcgagca cgacaggcag cgactggatc ttgcgattca
    22561 ttggtgctcc gcgtttgtag tgaatcgttg tgccggcaat cccagccatt ccagcgcggc
    22621 gccatgatag agccgctcgc gctgggccgg gcggagttcg agcgcttcga tgccgctgcc
    22681 ggggtgctgt tcaccgaggg ggaacggata gtcggtgccc agcatcaccc ggtcggcgcc
    22741 gacgacgtcg agaagatagc gcagcgcctg cggatcgtgc acgcacgagt cgaagaacag
    22801 gcgctggaag tattcgcgtg gttcgcggga gttgtcgacg gcgaccaggt ccgggcgcat
    22861 ccggaaaccg tgttcgatcc gtccgatcgt gaaggggaac gatccgccgc cgtgggcgaa
    22921 ggcgacgcgc agcccgggca ggcgttccag cacgccaccg aagatcaggc agcaggcggc
    22981 gcgcgactgc tcggccggca tcccgacgag ccagggcagc cagtacttcg gcatggtttc
    23041 cttgcccatc atgtcccatg gatggatcag gatcgcggcg ccgaggtccg ccgccgct
//
LOCUS       DASSNH010000021        16172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_233543, whole genome shotgun sequence.
ACCESSION   DASSNH010000021 DASSNH010000000
VERSION     DASSNH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 16172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16172
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233543"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFK00_00650"
     CDS             complement(<1..422)
                     /locus_tag="VFK00_00650"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET7591495.1"
                     /translation="MSDSPRIIYTLTDEAPFLATASLLPIVDAFTDSAGIKVETRDIS
                     LAGRILSQFPGVLKEGQNVSDDLAELGRLALEPSANIIKLPNISASLPQMKAAIRELQ
                     GKGFALPDYPDEPKNDAERETKARYDKVKGSAVNPVLR"
     gene            complement(603..1319)
                     /locus_tag="VFK00_00655"
     CDS             complement(603..1319)
                     /locus_tag="VFK00_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7591496.1"
                     /translation="MSVNLKLARVVDGALDALLPSHCLLCGAKGQGALSLCSACEAEM
                     PRNDIHCARCALPLESSAALCGRCIKREPPWDAAWAPFRYEWPLAQLESRFKFGGDLA
                     AGRTLALLWCAAPRPIGLPGAIVPVPLHRSRLRRRGYNQVLELAGTLARQLGVRVCRD
                     ALVRDRATNAQTELSAVQRRRNVRGAFGANLKGEVPAHVAVLDDVCTTGATLAECARV
                     LKRAGVQRVDAWALARAPAH"
     gene            1379..2410
                     /gene="bioB"
                     /locus_tag="VFK00_00660"
     CDS             1379..2410
                     /gene="bioB"
                     /locus_tag="VFK00_00660"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET7591497.1"
                     /translation="MQVDAAVRHDWAQAEIEALFSLPFMDLLGRAHAVHAAHHDPNAV
                     QVSTLLSIKTGGCPEDCAYCPQAQRYHTGVEAQKLMDVEDVVDRARAAKAAGASRFCM
                     GAAWRGPKDRDIPKVAEIIREVKALGMEACATLGLLGEGHAQALKDAGLDYYNHNIDT
                     SPDYYGEIIHTREFDDRLDTLEDIRAAGLKTCCGGIVGMGETRAQRAGLLRALANLPE
                     HPQSVPINQLVPVPGTPLADAEPLDPFEFVRTIAVARIVMPRSIVRLSAGRQQMSDEL
                     QALCFFAGAGSIFMGEKLLTTGNPDVAHDHALFARLDLHPMQVDVETHDTETVHADIL
                     ESCHSDAAA"
     gene            2489..3676
                     /gene="bioF"
                     /locus_tag="VFK00_00665"
     CDS             2489..3676
                     /gene="bioF"
                     /locus_tag="VFK00_00665"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET7591498.1"
                     /translation="MSNRADILQSLAARVEERQRAGLLRRARTLDAARGVHIAVDGRE
                     LINFCSNDYLGLAQDPRITRALQEAANDCGVGSTAAHLVCGHRREHARLEEALCEWTG
                     RDAAILFSSGWLANLGAMQALLDRDAVCVQDKLNHASLLDAAKLAGAALKRYPHRDVA
                     GAERQLAAAGEHPALLATDGVFSMDGDVAPLRELATLCKRDGATLMVDDAHGLGVLGT
                     HGAGSVAEAGLAEHDVPLLMATLGKALGCAGAFVAGSRALVEGLTQFARTFVYTTAMP
                     PALAAATCEAVHIARRDDWRREKLRALIARFRSGAMELGLPLADSMTAIQPIVLGDAA
                     RTVAASISLEGRGFLVTAIRPPTVPAGSARLRVTLSAAHEEQDVDRLLDALSLQALFP
                     IAS"
     gene            3774..4604
                     /gene="bioH"
                     /locus_tag="VFK00_00670"
     CDS             3774..4604
                     /gene="bioH"
                     /locus_tag="VFK00_00670"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HET7591499.1"
                     /translation="MKASPLTPTLSPDQAGREGDKLFIHTEGSGDTHIVLLHGWAMHG
                     GVFAPLVEALRGRCTLHVVDLPGHGRSSDSKIPLQPAACARAIAERVPPAIWLGWSMG
                     GLYALQAALDFPAQVRGVAMLCASPRFVRGEDWSYAVSPEILQGFGRDLESNYEGTLE
                     RFLALEALGSDCAQADLRKLRADTFSEHRPNVRALVEGLDVLEHTDLRNRLHEMTQSS
                     AWIAGRRDRLIPWQAMRWAAHQCGGAFTCVAGGGHAPFIGHTAEVIAALEPLLAEARA
                     "
     gene            4601..5482
                     /gene="bioC"
                     /locus_tag="VFK00_00675"
     CDS             4601..5482
                     /gene="bioC"
                     /locus_tag="VFK00_00675"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HET7591500.1"
                     /translation="MKDGWLDLRQVRRAFARSARDYERHDVLQREVESRLFESLDYFE
                     GSPARVLDVGSGTARGTARLKKRWKQAEVIALDVSLPMLREARRNAGWRKAFDRVAAD
                     ALALPFPDRSLDVVYSNLCMQWCEESRSFLAELRRVLKPGGLLVASSLGPDTLRELRA
                     AWDEVDPGQPRVGRFADMHQVGDAAIAAGLKDPVLDSGHIVLEYPDVRGLLHDLKGLG
                     ATNADCGRPRGLTGKSRFRAMVDAYERERRNGKIPATWEVVTMHAWGMPEGAMPLYGG
                     RDTRFEVIQRREPRPRT"
     gene            complement(5679..6560)
                     /locus_tag="VFK00_00680"
     CDS             complement(5679..6560)
                     /locus_tag="VFK00_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7591501.1"
                     /translation="MATIRIGISGWRYPPWRGVFYPKDLVQRDELSYASRHFPSVEIN
                     GSFYSLQRPESYARWREATPPGFVFAVKGPRFITHIKRLRDFEVPLANFFASGVFELR
                     EKFGPVLWQFPANFKYDHDVFARFFDRLPRDTEAALKLARRRDFRMTGRARLAIDRNR
                     KMRYAIEIRHPGFLAPSFVALLREHGIALVVADTAGKWPYVEELTANFMYLRLHGDKQ
                     LYASGYSEEALDRWAGRIDAWSSGRQPSDAVRVPDAPAPPRRKSRDVYCYFDNDMKVK
                     APFDALRLMEMLDLHWR"
     gene            6790..7674
                     /locus_tag="VFK00_00685"
     CDS             6790..7674
                     /locus_tag="VFK00_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7591502.1"
                     /translation="MTARNPERAAWTALAVLTIIWSLNWTVMKVATRDAGPFTFSAQR
                     YVIGTVVLFALLAVRRRSLEPTPWLATIAIGLCQTTGFQALAQWALVSGGAGKTALLA
                     YTMPFWVVPLAWWWIHEKPGLKRWICIALAAVGFACVIQPWQPIGAPHSIAFAILAGL
                     AWAIATVLSKRLFQKHPDVTPLRLTAWQMLFGTIGLVILALAVPQRPVAWTGEYVAAL
                     LYNGLLSSGVCWVLWALIVQRLSANAAGLTSLAVPVAGVLFAWWLLAERPAPAEWLGI
                     GLIAAALAILNFSRRVAA"
     gene            7741..9033
                     /gene="tviB"
                     /locus_tag="VFK00_00690"
     CDS             7741..9033
                     /gene="tviB"
                     /locus_tag="VFK00_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HET7591503.1"
                     /translation="MADAALPRPEEMRLAVIGLGYVGLPLAVGFGRKLPTLGFDVNAA
                     RIEELKQHRDHTLEVTPDELRAAGQLRYSHDPADLKGCNIFIVTVPTPVGRGKRPDLT
                     PLKSASTTVGRAIGRGGVVIFESTVYPGATEEVCVPIIEEESGLKFNQDFYAGYSPER
                     INPGDKEHRLDTIRKVTSGSTPEVADFVDALYRRVVSAGTHKASSIKVAEAAKVIENT
                     QRDLNIALINELALIFHRLHIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTH
                     KAQEIGYHPEVILAGRRINDGMGQHVAERVIKLMLQKRINVAGAKVLVLGLTFKENCP
                     DLRNTRVTDIIDEFKACHADIDVYDPWVDAAEARREYGVEVVPKPAPGSYDAIVLAVA
                     HHQFTDMGPAALRALGKTECVLFDVKNALPRGAADDRL"
     gene            9272..10279
                     /locus_tag="VFK00_00695"
     CDS             9272..10279
                     /locus_tag="VFK00_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HET7591504.1"
                     /translation="MRVLVTGAAGFIGSALSLRLLARGDEVLGVDNLNDYYDVSLKQA
                     RLDRLTPQAAFQFAKVSIEDRSALERAFADFEPQRVVNLAAQAGVRYSLENPYAYVEA
                     NLVGFINILEACRHGKVGHLVYASSSSVYGANRKLPFSVQDSVDHPVSLYAATKKANE
                     LMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTRKILAGEPIDVFNHGHHTRDF
                     TYIDDIVEGVIRTLDHVATPDPDYDPLHPNPGTSSAPYRLYNIGNSNPVQLFRYIEVL
                     EQCLGRKAEQNLLPLQPGDVPDTSAEVTELMTDTGYKPDTSVEEGVKRFVEWYRDYYG
                     V"
     gene            complement(10359..11360)
                     /gene="galE"
                     /locus_tag="VFK00_00700"
     CDS             complement(10359..11360)
                     /gene="galE"
                     /locus_tag="VFK00_00700"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7591505.1"
                     /translation="MNSPQAQRNRAILVTGGAGYIGSHVVLQLVEAGERVVVLDDLST
                     GFREAVIGAELVVGNVGDRGLVATLLRDQGVDTVMHFAARTIVPESVADPLRYYGNNT
                     CATRNLLECCRDAGVRQIVFSSTAAVYGIPEGGKAAEDTPTAPINPYGSSKLMSEWML
                     RDLAAAGGPRYVALRYFNVAGCDPAGRIGQSTAKATLLVKVACEAALGKRPRLSIFGT
                     DYDTPDGTGVRDYIHVDDLARAHLDALGYLRDGGASTTLNCGYGHGYSVREVLEAVGR
                     AHGAPVPFVEEPRRAGDPPSLVAVSDRIRGTLGWQPRHDDLDFIAQTALAWERKLPA"
     gene            11740..12450
                     /locus_tag="VFK00_00705"
     CDS             11740..12450
                     /locus_tag="VFK00_00705"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HET7591506.1"
                     /translation="MKAWKKGTLAALGLLVGGLFAGIPSAWATPQNFSFTGTFNQDDN
                     VQLFHFTVGAGSTVVLRTWSYAGGTNAAGDAIAAGGFDPILALFDGTGAYIDQNDDGG
                     CGSVDPDAMTGECWDTYFSAALAPGDYTVAVMEYDNFANGPNLSDGFERDGEGNFTKA
                     AGWCDDDSAPNAVGFCDVSGTPGGARDNHWAFDLLGVESATQQPPQVGVPEPSELGLM
                     GGGLLLLFWLSRRRWHRA"
     gene            12743..13402
                     /locus_tag="VFK00_00710"
     CDS             12743..13402
                     /locus_tag="VFK00_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7591507.1"
                     /translation="MNKRIAAGLVLAAGMLVGGIATATPITMDFDGLTPGVSVDNYYD
                     GGCSKFLGMDMDCGGPDYGVVWNGAGVRDLDANQPSDPNFMVPTGPGGFLTTAAIMDV
                     ADGFDSRLSFYYSAVTAGDVSVFSGADGSGQLLTSLDLGTSGNQCGDVPCWDFVDLSF
                     QGTAKSVVFRGFLGLVGFDNVTLGANPQAVPEPAALGMFGLGVLLIGMFAGMRKRARQ
                     N"
     gene            13616..14422
                     /locus_tag="VFK00_00715"
     CDS             13616..14422
                     /locus_tag="VFK00_00715"
                     /inference="COORDINATES: protein motif:HMM:NF038132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038132 family protein"
                     /protein_id="HET7591508.1"
                     /translation="MKVMGRKFWGPLALFAGLLACGSAAATTIPSGWSCDGNCGTNGA
                     DGVVTAPPNGQTSYQWISTWSGNDGVGVLPSGGLSGEVNGSTLATSAFSADAGTSLNF
                     YFNYVTSDGGDYSDYAWAALFNEDGTLNALLFTARTAPSGSIVPGFGMPAVNPGVTLN
                     PSSVDIIGGGPNWSPLGSDSGECWSTGCGYTGWVNANYTIATAGNYYLQIGVINWSDS
                     GYDSGLAMSGVTVGGAPINGVPEPAALGMFGLGALLVGLFAGLRRRVPQI"
     gene            14654..15349
                     /locus_tag="VFK00_00720"
     CDS             14654..15349
                     /locus_tag="VFK00_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7591509.1"
                     /translation="MNMLRKQVGLALVMVAGMLACGVASAAVVMTFDQQNGNGEYVDN
                     YYNGGCGGSYGGGSVTCGGPNYGVVWNGALEGYAPNGLWNNTSNSSSPPGVIGFLDSD
                     NAYMNVAAGFDTGFSFYYAAANVGGTIEVWSGLNGTGTMLASLSLPVTGAGCDGITSF
                     SCWDPIGVTFAGTAMSVNFAGTANQIVFDNVTIGSGTPINGVPEPAALGMFGLGALLV
                     GLFAALRRRALQS"
     gene            15587..>16172
                     /locus_tag="VFK00_00725"
     CDS             15587..>16172
                     /locus_tag="VFK00_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591510.1"
                     /translation="MNALTKRMGFAAILVGSLLACGIASATTVMTFDEFNPNGEYVGN
                     YYNGGCGDSYNGGSVTCGGPNYGVTWTHAIAGGAPGGLFNNVDLEPSSPGVIGGFDDD
                     TGSFMNVAAGFDTGFSFYYAAANTAGSINVYSGLNGTGTLLASLALPVNGSNCNGSFA
                     AYSCWTPIGVAFDGIAQSVNFGGAAFYIAFDNVTL"
BASE COUNT         2759 a         5342 c         5252 g         2819 t
ORIGIN      
        1 tggcgcagca cgggattgac cgcgctgccc ttgaccttgt cgtagcgcgc cttcgtctcc
       61 cgttcggcat cgttcttcgg ctcgtcggga taatcgggca gcgcgaagcc cttgccctgc
      121 aattccctga tcgccgcctt catctgcggc agtgacgccg aaatgttcgg cagcttgatg
      181 atgttggcgg aaggctccag cgccagccgg ccgagctcgg ccaggtcgtc ggaaacattc
      241 tggccttcct tcagcacccc cggaaactgc gacaggatgc gcccggccag cgaaatgtcg
      301 cgcgtctcga ccttgatccc ggccgagtcc gtgaaggcgt cgacgatcgg cagcagcgaa
      361 gccgtggcga ggaacggtgc ctcgtcggtg agcgtgtaga tgatcctggg agagtcggac
      421 atggaaagct gatcccgctg cggacaagac ctccattgtc gcgttcaccc tcttccaaag
      481 gaagggggtc gccgcgccag cggcgggggg atgtgccgca ggcccgaaaa gccatctccc
      541 tcggtcgcgc tgcgcgcgcg ttcgctttgc tccgcccttc gtcccgaagg ggggaagaaa
      601 aactagtgtg ccggcgcgcg tgccaatgcc caggcatcga cgcgttgtac cccggcgcgt
      661 ttcagcaccc gcgcgcactc ggccagcgtc gcgccggtcg tgcatacatc gtcgagcacc
      721 gcgacgtgcg ccgggacctc gcccttcaga ttcgcgccga aggcaccacg cacgttgcgg
      781 cggcgctgga cggcgctcaa ctcggtctgg gcattggtgg cgcggtcgcg cacgagcgcg
      841 tcgcggcaca cccgcacgcc gagctgcctg gccaaggtcc cggccagctc gagtacctgg
      901 ttgtagccgc ggcggcgcag tcgcgaacga tgcagcggca ccggcacgat agccccggga
      961 agcccgatcg ggcgtggcgc cgcgcaccac aagagcgcaa gggtacgccc ggctgcgaga
     1021 tcgccgccga acttgaagcg tgactcgagt tgcgcgagcg gccattcgta gcggaacggc
     1081 gcccatgccg cgtcccaggg tggctcgcgc ttgatgcagc gcccacacaa cgccgccgac
     1141 gattccagcg gcagtgcaca gcgcgcacaa tggatgtcgt tgcgtggcat ttccgcttcg
     1201 catgcggagc acaggctcaa cgcaccttgg cctttggcgc cacacagcag gcaatgcgat
     1261 ggcaacagcg catccagtgc gccgtcaacc acccgggcca gtttcaggtt gacagacatc
     1321 tcccccgcct ccgacacttc tcccttcact ataaagcgga gctgccgcga tgaagggaat
     1381 gcaggtcgac gccgcggttc gccacgactg ggcgcaagcc gaaatcgaag cgcttttctc
     1441 gctccccttc atggatctgc tcggtcgggc ccacgccgtg cacgcggccc atcacgatcc
     1501 gaacgccgtg caggtttcga cgctgctgtc gatcaagacc ggcggctgcc cggaggattg
     1561 cgcgtactgc ccgcaggcac agcgctacca caccggcgtc gaagcccaga agctgatgga
     1621 cgtggaggac gtcgtcgacc gtgctcgtgc cgccaaggcc gcgggcgctt cgcgcttctg
     1681 catgggtgcg gcatggcgcg ggcccaagga tcgcgacatc ccgaaggtgg ctgagatcat
     1741 ccgcgaggtg aaggcgctcg gcatggaagc ctgcgcgacg ctgggcctcc tcggcgaggg
     1801 ccatgcgcag gcactcaagg atgccgggct cgactactac aaccacaaca tcgacacctc
     1861 gccggactac tacggcgaga tcatccacac ccgcgagttc gacgaccggc tggacacgct
     1921 ggaagacatc cgcgccgcgg gcctgaaaac ctgctgcggc ggcatcgtcg gcatgggcga
     1981 gacgcgcgcc cagcgcgccg gcctgctgcg cgcgctggcc aacctgcccg agcatccgca
     2041 atcggtgccg atcaaccagc tcgtgccggt gcccggcacg ccgcttgcgg acgccgagcc
     2101 gctcgatccc ttcgagttcg tgcgcacgat cgcagtcgcg cggatcgtga tgccgcgttc
     2161 gatcgttcga ctttcggccg ggcgccagca gatgagcgac gaactgcagg cactgtgctt
     2221 ctttgcgggc gccggctcca tcttcatggg cgagaaactc ctcaccaccg gcaatccgga
     2281 cgtcgcgcac gaccatgcgc tgttcgcacg cctcgacctg catccgatgc aggttgatgt
     2341 ggaaacgcac gacacggaaa cggtgcatgc cgacatcctg gaatcgtgtc actccgatgc
     2401 cgccgcttga agcagtccct cctccctccg ggagaagggc gcaacgcgct gcctctcccg
     2461 gagggcgagg ggcttttcaa gcggcagcat gagtaaccgc gccgacattc tgcaatcgct
     2521 ggctgcgcgc gtcgaagagc gccaacgcgc aggactgctg cgccgtgcac gtacgctcga
     2581 cgccgcgcgc ggcgtacata ttgccgtcga tggtcgcgag ctcatcaatt tctgcagcaa
     2641 cgactacctc ggcctcgcgc aggacccgcg gatcaccaga gcgcttcagg aagccgcgaa
     2701 cgactgcggc gtgggttcca ccgccgcgca cctggtgtgc ggccatcgcc gcgagcacgc
     2761 gcggctggag gaagcgctgt gcgaatggac cggtcgcgat gctgcgatcc tgttctcttc
     2821 cgggtggctt gcgaacctcg gcgcgatgca ggcgctgctg gatcgcgacg ccgtatgcgt
     2881 gcaggacaag ctcaaccatg cgagccttct ggacgccgcg aaactcgccg gcgccgcgtt
     2941 gaagcgttat ccgcatcggg acgtggccgg tgccgaacgg cagctcgccg ctgcgggcga
     3001 acatccggcc ttgctcgcga ccgacggcgt gttcagcatg gacggcgacg tcgcaccgtt
     3061 gcgcgaactc gcgacgctgt gcaagcgcga cggtgccacg ctgatggtcg atgacgccca
     3121 cggcctcggt gttctcggca cgcacggcgc cggcagcgtg gccgaggcag gcttggcgga
     3181 gcatgacgtg ccgctgttga tggcgaccct cggcaaggcg ctgggttgcg caggtgcgtt
     3241 cgtcgccggc agccgcgcgc tggtcgaggg actgactcag ttcgcgcgca cgttcgtgta
     3301 caccaccgcg atgccgcctg cgctcgccgc ggccacgtgc gaggcggtgc acatcgcgcg
     3361 cagggacgac tggcggcgcg agaaactgcg cgccttgatc gcgcgctttc gttccggcgc
     3421 aatggagctc ggcctgccac tcgcggactc gatgaccgcg atccagccga tcgtgctggg
     3481 cgatgccgcg cgcactgtgg ccgcgtcgat ttcgctggag ggccgaggct tcctggtcac
     3541 cgcgatccgt ccgccgacgg tgccggccgg cagcgcccgc ttgcgggtga cgctttccgc
     3601 cgcgcatgaa gaacaggacg tggatcggct gctcgacgcc ttgtcgcttc aagccctttt
     3661 ccccatcgct tcatgatggg gagagagctg gatgaggggg tgcagcccga attccgacac
     3721 acctacgaac gctccgacaa atcacatcaa cgattcatcc tgcattacgc cctatgaagg
     3781 ccagccccct caccccaacc ctctcccctg atcaagccgg gcgagaggga gacaagcttt
     3841 tcatccatac cgaaggctcc ggcgacaccc atatcgtgct gctgcacggc tgggcgatgc
     3901 atggcggcgt gttcgcgccg ctggtcgagg cgctgcgggg ccgctgcacg ctgcacgtgg
     3961 tggatctgcc agggcatggc cgctcgtccg actcgaaaat cccgctgcaa ccggcggcgt
     4021 gcgcgcgcgc gattgccgag cgcgtgccgc cggccatctg gctgggctgg tcgatgggcg
     4081 ggctgtatgc gctgcaggcg gcgctggatt ttccggcgca ggtgcggggg gtcgccatgc
     4141 tgtgcgcgtc gccgcggttc gtgcgtggcg aggactggtc gtacgcggta tcacccgaaa
     4201 tcctccaggg cttcggccgc gacctcgaat ccaactacga gggtacgctg gaacgcttcc
     4261 tcgcgctgga agcgctgggc agcgactgcg cgcaggcgga ccttcgcaag ctgcgtgccg
     4321 acaccttcag cgaacaccgc ccgaatgtgc gtgcactggt cgaaggcctg gacgtcctcg
     4381 aacacaccga cttgcgaaac cgcctgcatg aaatgacgca atcgagcgcg tggatcgccg
     4441 ggcgtcgcga ccgcctgatc ccctggcagg cgatgcgctg ggccgcccat caatgcggcg
     4501 gcgcattcac ctgcgtcgcg ggcggaggac acgcgccgtt cattggacat acggccgaag
     4561 tgatcgcggc gctcgaaccg ctgctggcgg aggcgcgggc atgaaggacg ggtggctgga
     4621 tcttcgccaa gtgcgccgtg cattcgcgcg ttcggcccgt gactacgagc gccacgacgt
     4681 actgcaacgc gaagtcgagt cgcgcctgtt cgaaagcctg gattacttcg aaggcagccc
     4741 cgcacgcgtg ctcgacgtcg gctctggcac cgcgcgaggc acggcccggc tgaagaagcg
     4801 ctggaaacag gccgaggtca tcgctctgga cgtgtcgcta ccgatgctgc gcgaggcgcg
     4861 ccggaatgca ggctggagga aggcattcga ccgcgtcgcg gcggacgccc tcgcgctgcc
     4921 gtttcccgat cgcagtctgg acgtcgtcta ttccaacctg tgcatgcaat ggtgcgagga
     4981 gtcccgctcg ttcctagccg aacttcgccg ggtgctgaag cctggcgggc ttctggtcgc
     5041 ctccagcctc ggccccgaca ccctgcgcga gctgcgcgcg gcgtgggacg aagtcgatcc
     5101 ggggcagccg cgcgtcgggc ggttcgccga catgcaccag gtcggcgacg ctgcgatcgc
     5161 ggcgggactc aaggatccgg tgctggatag cggccacatc gtcctcgagt acccggatgt
     5221 acgcggcctg ttgcacgacc tcaaagggct cggcgcaacc aacgcggact gcggccgccc
     5281 gcgcggcctg accggcaagt cccgattccg cgcaatggtg gacgcctacg aacgcgagcg
     5341 ccggaacggc aagatcccgg cgacctggga ggtcgtcacc atgcatgcct ggggcatgcc
     5401 ggaaggcgcg atgccgttgt acggcgggcg cgatacgcgg ttcgaggtca tccagcggcg
     5461 cgaaccgcgg ccgcgcacct gaggcgctgt gcccccgcgt cgatcattgc ctgcccttcg
     5521 gcaccgccag gacgacgaag ctccccggct ccactgtggt cgcgggtgcg gactgctctg
     5581 cttcccgcgg gccgtggccc gaccacaccg gcctgcgctt tcgccttttt ttggaaatgc
     5641 ctgggctcac ggcgtacagc ctccgcagct cgtcaccctc accgccaatg caggtcgagc
     5701 atttccatca atcgcagcgc atcgaagggc gccttcacct tcatgtcgtt gtcgaagtaa
     5761 cagtacacgt cccgcgactt gcggcgtggc ggcgcggggg cgtcgggtac gcgcacggcg
     5821 tcggacggtt gcctgcccga actccaggca tcgatgcgtc cggcccagcg atcgagcgcc
     5881 tcctcgctgt aaccgctggc atacagctgc ttgtcgccgt gcaggcgcaa gtacatgaag
     5941 tttgccgtga gctcttccac gtacggccac ttgcccgccg tgtccgccac caccagcgcg
     6001 atgccgtgtt cccgcaacag tgcgacgaat gacggagcga ggaaacccgg atggcgaatc
     6061 tcgatcgcgt agcgcatctt tcggttgcga tcgatcgcca atcgtgcgcg gcctgtcatg
     6121 cggaagtcgc gccggcgcgc cagcttcagc gcagcttcgg tatcacgcgg caggcggtcg
     6181 aagaaacgcg cgaatacatc gtggtcgtac ttgaagttgg cggggaactg ccagagcacc
     6241 ggaccgaatt tttcacgcaa ctcgaacacg ccggacgcga agaaattcgc cagcggcacc
     6301 tcgaagtcgc gcagccgctt gatgtgggtg atgaagcgcg gccccttcac cgcgaacacg
     6361 aatccgggcg gcgtggcctc gcgccaacgc gcgtaacttt ccggccgctg cagcgaatag
     6421 aacgaaccgt tgatttcgac gctcggaaag tgccgcgagg cgtaggacaa ctcgtcccgc
     6481 tgcaccaggt ccttcggata gaacacgccg cgccagggcg ggtagcgcca tccggaaatg
     6541 ccgatgcgga ttgtggccat gcgtccctgc ctgctccggc cagatgggcc gagacgctat
     6601 cctacgcgtc ttcgtggcta cgaaacctct agtccaatcc gctccgcttc tctccttccc
     6661 cttcaggggg aaggccgggg tgggcgtggg gtacacgccg gtgccgatcc catcgtcaac
     6721 cccctctcca ccgaaccctc cccttgaagg cgagggctcc caggcaacgc agatctcgcg
     6781 atcgcatcca tgacggcccg caatcccgaa cgcgccgcgt ggaccgcgct tgcggtcctc
     6841 acgatcatct ggagcctcaa ctggacggtc atgaaggtcg cgacgcgcga cgccgggccc
     6901 ttcaccttca gtgcgcagcg ctacgtgatc ggcacggtcg tcctgttcgc gctgctcgcg
     6961 gtccggcgcc ggagcctcga gccaacgccg tggctcgcga cgatcgcgat cggcctttgc
     7021 cagaccaccg gattccaggc gctcgcccaa tgggcgctcg tgtcgggcgg cgccggcaag
     7081 accgccctgc tggcctacac gatgccgttc tgggtggtgc cgctcgcgtg gtggtggatc
     7141 cacgagaagc ccggcctgaa gcgctggatt tgcatcgcac tggccgcggt cggctttgcg
     7201 tgcgtgatcc agccgtggca gccgatcgga gcgccgcaca gcatcgcctt cgcgatcctc
     7261 gcggggctcg cgtgggccat cgcgacggtg ctgtccaagc gcctgttcca gaagcacccg
     7321 gacgtgacgc cgctgcgcct caccgcgtgg cagatgctgt tcggcacgat cgggctggtg
     7381 atcctcgcgc tcgcggtgcc ccagcgccca gtcgcatgga cgggcgaata cgtcgcggcg
     7441 ctcctctaca acggactgct ttcctcgggc gtgtgctggg tgctttgggc gctgatcgtg
     7501 cagcggcttt cggcgaacgc ggccggcctc accagcctcg cggtcccggt ggctggcgtg
     7561 ttgtttgcat ggtggttgtt ggccgagcgg ccggccccgg cggaatggct cggaatcggc
     7621 ttgatcgccg ctgccctcgc cattttgaat ttttcgcgcc gcgtggcagc ataagccgct
     7681 ttgcggcgcg gcctcgccgc atgtcaagga ttctccatcc accaccaagg aaagcgatcg
     7741 atggctgatg ccgcactgcc ccgtccggaa gaaatgcgcc tggccgtcat cggcctgggt
     7801 tacgtgggcc tgccgctggc ggtgggcttc ggccggaaac tgcccacgct gggtttcgac
     7861 gtcaacgccg cccggatcga ggagcttaaa cagcaccgcg accatacgct cgaggtcaca
     7921 ccggacgaac tgcgtgcagc ggggcaactg cggtacagcc atgatccggc agacctcaag
     7981 ggctgcaata tcttcatcgt caccgtgccg accccggtcg gccgcggcaa gcggccggac
     8041 ctgacgccgc tcaagagcgc cagcaccacc gtcggccgcg ccatcggccg cggcggcgtc
     8101 gtgatcttcg aatcgaccgt ctatccgggc gcgaccgaag aggtgtgcgt gccgatcatc
     8161 gaggaagagt cggggctgaa gttcaaccag gatttctacg ccggctacag cccggaacgg
     8221 atcaacccgg gcgacaagga gcaccgtctc gacaccatcc gcaaggtcac gtcgggctcc
     8281 acgcccgagg tcgccgattt cgtggacgcg ctgtaccggc gagtggtcag cgccggcacc
     8341 cacaaggcgt cctccatcaa ggtcgccgag gccgccaagg tgatcgagaa cacccagcgc
     8401 gacctcaaca tcgcgctgat caacgagctg gcgctgatct tccatcgcct gcacatcgat
     8461 accctggaag tgctggaggc ggccggcacc aagtggaact tcctgccgtt ccggccgggc
     8521 ctggtcggcg gtcattgcat cggcgtcgat ccctattacc tcacccacaa ggcccaggaa
     8581 atcggctatc accccgaggt gatcctggcc ggtcggcgga tcaacgacgg catgggccag
     8641 cacgtggccg agcgcgtgat caagctgatg ctgcaaaagc ggatcaacgt ggcgggtgcg
     8701 aaggtgctgg tgctgggact caccttcaag gaaaactgcc ccgacctgcg caacacccgg
     8761 gtcaccgaca tcatcgacga gttcaaggcc tgtcacgccg acatcgacgt ctacgatccg
     8821 tgggtcgacg ccgccgaggc gcgccgcgaa tacggcgtcg aggtcgtacc gaaaccggcc
     8881 ccgggcagct acgacgcgat cgtgctggcg gtggcacacc atcagttcac cgacatgggc
     8941 ccggccgcgc tgcgggcgct gggcaagacg gagtgcgtgc tgttcgacgt caagaatgcg
     9001 ctgccgcgcg gcgcggcgga cgatcggctg taggcggacc gttgaactgc ccggaccgcg
     9061 gcgagcaatc ggagcaaact cccctctccc tccgggagag gataccccgc aggggcgggt
     9121 gagggtacgc acccgcacat ggcagcatgg gagagtgcaa cccgcgaggg gccgtaccct
     9181 catccggcgc ttcgcgccac cttctcccga ggggagaagg aaaagccgtc tagactcgcg
     9241 tttggcaaga tcaggaaatt gaggagattt cgtgcgcgta ctcgtcaccg gcgccgccgg
     9301 cttcatcgga tccgccttga gccttcgcct cctcgcgcgc ggcgacgagg tgctcggcgt
     9361 cgacaacctc aacgattact acgacgtcag cctgaagcag gcgcgtctgg atcggctgac
     9421 tccacaggcc gcgttccagt tcgccaaggt cagcatcgaa gaccgctccg cgctcgagcg
     9481 cgcattcgcc gacttcgaac cgcagcgtgt cgtgaacctc gcggcgcaag ccggtgtgcg
     9541 ctactcgctc gagaacccct acgcctacgt cgaggcgaac ctggtcggct tcatcaacat
     9601 cctcgaggcc tgccggcacg gcaaggtcgg gcacctggtg tacgcctcat cgagctcggt
     9661 gtacggcgcc aaccgcaagc tgccgttctc cgtgcaggat tcggtggacc atccggtcag
     9721 cctgtacgcg gccaccaaga aggccaacga attgatggcc cacacctaca gccacctgta
     9781 cggccttccg accaccggat tgaggttttt cacggtgtac ggcccgtggg gccgacccga
     9841 catggcgctg ttcctgttca cccgcaagat cctcgcgggc gagccgatcg acgtgttcaa
     9901 ccacggccac cacacgcgcg acttcaccta catcgacgac atcgtcgaag gcgtgatccg
     9961 cacgctggac cacgtggcca cgcccgatcc ggactacgac ccgctgcacc ccaatccggg
    10021 cacgtcctcg gcaccctacc ggctctacaa catcggcaac agcaatcccg tccagctctt
    10081 ccgttacatc gaggtgctgg agcagtgcct cggccgcaag gccgagcaga acctgctgcc
    10141 gctgcagccg ggcgatgtcc ccgacaccag cgcggaagtc acggagttga tgaccgacac
    10201 cgggtacaaa cccgatacgt cggtggaaga aggcgtgaag cgattcgtcg agtggtaccg
    10261 ggattactac ggggtttgac ggaaagccga agagccatcc ccctcggtcc cccttccctt
    10321 ggaaggggga agacaagcaa tgccattccg cttcacgatc acgccggcag cttgcgctcc
    10381 cacgcgagcg ccgtctgcgc gatgaaatcg aggtcgtcgt ggcgcggctg ccagcccagc
    10441 gtgccacgga tccggtccga aaccgcgacc agcgaaggcg gatcgccggc gcggcgcggc
    10501 tcttccacga acggcaccgg cgcgccatgc gcgcgtccca ccgcttccag cacttcgcgc
    10561 accgagtacc cgtgtccgta gccgcaattg agggtcgtgg atgcgccgcc gtcgcgcaga
    10621 tagccgaggg catccaggtg cgcgcgcgca aggtcgtcca cgtggatgta gtcgcgcaca
    10681 ccggtgccat cgggggtgtc gtagtcggtc ccgaagatcg acagccgcgg ccgcttgccg
    10741 agcgccgcct cgcaggcgac cttcaccagc aacgtcgcct tcgcggtcga ctggccgatg
    10801 cggccggccg gatcgcagcc ggccacgttg aaatacctga gtgccacgta gcgcgggccg
    10861 cccgccgccg ccaggtcgcg cagcatccat tcgctcatga gcttggagct gccatacggg
    10921 ttgatcggtg cggtcggcgt gtcctcggcg gccttgccgc cctcgggaat accgtaaacc
    10981 gccgcggtcg aggaaaacac gatctgccgc acgccggcgt cgcggcagca ctccagcaga
    11041 ttgcgggtgg cgcaggtgtt gttgccgtaa tagcggagcg gatcggccac cgattccggc
    11101 acgatcgtgc gcgccgcgaa atgcatcacc gtatcgaccc cttggtcgcg cagcagcgtc
    11161 gccaccaagc ccctgtcgcc gacgttgccg accaccagtt ccgcaccgat cacggcctcg
    11221 cggaacccgg tggagaggtc gtccagcacc actacccgct ccccggcctc gaccagttgc
    11281 aggaccacgt ggctgccgat gtaaccggca ccgccggtga ccaggatggc gcggttgcgc
    11341 tgcgcttgcg gtgaattcaa cgttctctcc ttgggctcgg gtcggcgcgg gtcacatacc
    11401 cgcaacacaa gcgctgcatg gtacccgcga tcccggccgc gccgaatgca cgcgcggggc
    11461 gtcggtagac cttacatcgc cgaggccatg ccgcggcctc gaggggcacg cccgctcggc
    11521 cgtaaccggc cgctcccact cgattttcgt cccgggccgg ggcgcgcgga cacccccaag
    11581 gccggccggg cggggcgagg gcgtcggcca actttacatt ttggcgaaga tcgcctccgg
    11641 cgttcgcggc aatgcattga aacaaaagca tttattcagc ggcatggaaa atgcttcgga
    11701 acccgcgctt tttcgggact tcaatcagga gaatttctca tgaaagcatg gaaaaaggga
    11761 accctcgccg cgctcggatt gctcgtcggc gggctgttcg caggcattcc cagcgcctgg
    11821 gccacaccgc agaacttctc gttcacgggc acgttcaacc aggacgacaa cgtccagctg
    11881 ttccacttca cggtaggcgc ggggtccacc gtggtgctgc ggacctggag ctacgccggc
    11941 ggcaccaatg ccgcggggga cgccattgcg gcgggcggtt tcgatccgat cctcgcattg
    12001 ttcgacggca ccggcgccta catcgaccag aacgatgacg gcggttgcgg tagcgtggat
    12061 cccgatgcaa tgacgggaga gtgctgggat acctatttca gcgcagcgct cgcccccggc
    12121 gattacaccg tcgcagtcat ggagtacgac aatttcgcga acgggccgaa cctctcggac
    12181 ggcttcgagc gggatggcga aggcaacttc accaaggccg cgggctggtg cgacgacgac
    12241 agtgcgccca acgcggtcgg gttctgcgat gtttcgggca ctcctggcgg cgcccgcgac
    12301 aaccattggg cattcgacct cctgggtgtc gaatccgcga cccagcagcc cccacaggta
    12361 ggcgtgcccg aaccttcgga actcggactg atgggcggcg gactgctgct gctcttctgg
    12421 cttagccggc gtcggtggca tcgggcctga tcgacttgtt tcgcgttcct tcgaaagacc
    12481 ccgcttcggc ggggtttttc ttggccacgt cggtgcctcg cgcaccggac ggagggtccc
    12541 ggatcccggg cgcaccttcc cacgaggatt cactggcttg cgttgcgccc gtcgaaaatc
    12601 tttacacatt cggcatctac gctgagtcgt agtcaacata cgtagttgat ccgcaaatga
    12661 ttatctgtgc ggcacaggag ttgctttaca tgactcgccc tacagggggc gtgccaaaac
    12721 gcgaggacat gcaatgaatt cgatgaacaa acggattgcg gccgggctgg tgctggccgc
    12781 gggaatgctg gtgggcggca tcgccaccgc cacaccgatc acgatggatt tcgacggcct
    12841 gacgcccggt gtttccgtcg acaattacta cgatggcggt tgcagcaaat tcctggggat
    12901 ggacatggat tgcgggggtc cggattacgg cgtggtgtgg aacggcgcag gcgtgcgcga
    12961 tctcgatgcc aaccagccaa gtgatcccaa cttcatggtc cccactggcc ctggcggctt
    13021 tctgacgacg gcagcaatca tggacgttgc cgacggtttc gattcgcgcc tgtcgttcta
    13081 ctattccgcg gtgactgctg gcgacgtcag cgtattcagc ggagcggacg gtagcggcca
    13141 gctcctgaca tcacttgacc tcgggacctc aggcaatcag tgcggtgacg tcccgtgctg
    13201 ggacttcgtc gatctgtcat tccaggggac tgccaagtcg gtggtcttcc ggggctttct
    13261 gggcctcgtc gggtttgaca atgtgaccct cggcgcgaac ccgcaagccg tccccgaacc
    13321 cgccgcgctg gggatgttcg gccttggcgt cctgcttatc ggcatgttcg caggcatgcg
    13381 caagcgcgcg cggcagaact gaccaggcaa gctccagccc ctgctccagc aacccccggc
    13441 aacgggggtt gttttttggt tcttgaagca tcgatgttgt tgcgctcggc ctgtcggaga
    13501 agtttacgga atcagccatt cgctactcct gtggcagggt aaccgtttga aacaaaacga
    13561 gtaatgccat tggcacgcga aatgctgcaa cggtgtccac tatcgggagg tgatcatgaa
    13621 ggtgatggga aggaagttct ggggaccctt ggcgctcttc gccgggctgc tggcgtgcgg
    13681 cagcgccgcg gccaccacca ttccttcggg ctggagttgc gacggcaatt gcgggacgaa
    13741 cggcgccgat ggggtcgtga cggcgccacc caacggccag acgtcgtatc aatggatctc
    13801 gacatggagc ggcaatgatg gggtgggcgt gctgccgagc ggcggattga gcggggaggt
    13861 caatggctcc acgctcgcga cgtcggcgtt ttcggcggat gccggcacca gcctcaactt
    13921 ctacttcaac tacgtcacgt ccgacggcgg cgactacagc gactatgcct gggcggctct
    13981 gttcaacgag gacgggacac tgaacgcgtt gctcttcacc gcgcggacgg cacccagcgg
    14041 cagcatcgtc ccgggcttcg gcatgccggc ggtcaatccc ggcgtcacct tgaatccgtc
    14101 atctgtcgac atcatcggcg gcggccccaa ctggtcgccg ctcggaagcg attccggaga
    14161 gtgttggtca accggctgtg gctatacggg ttgggtcaac gccaactaca cgatcgcgac
    14221 ggccggcaac tattacctcc agatcggcgt catcaactgg agcgattcgg gttacgacag
    14281 cggactcgcc atgtcgggcg tgaccgtggg cggcgcgccc atcaatggtg tgccggagcc
    14341 ggctgctctg ggcatgttcg gcctgggcgc attgctggtc ggactgttcg ccggcttgcg
    14401 gcgccgcgtc ccgcaaattt gaccgagcgg tcacaaggtc ctgttcgaaa caacccccgg
    14461 cgacgggggt tgttttttgt ggcgccaaaa tgcgtgctga ttcgaccatt caaaaccgtc
    14521 gaaaggattt acacttcccg attttcgcga cctactggct gcgataaccg attgaattga
    14581 tatgcccctt tttgctggca cgggaattgc tcttttgttg cgaacagggc agcggtcgat
    14641 tcaggaggcg agtatgaata tgctcaggaa acaggtcggc ctcgcattgg tgatggtggc
    14701 cggcatgctg gcatgcggcg tagcgagcgc cgcagtggtg atgacgttcg atcaacagaa
    14761 cggaaacggg gaatacgtcg acaattatta caacggcggc tgtggcggct cctacggtgg
    14821 cggcagtgtt acttgcggcg gccccaacta cggcgtggtt tggaatgggg cgctcgaggg
    14881 ctacgcgccc aacggcctgt ggaacaacac gtccaattca tcgagccccc cgggcgtgat
    14941 cggcttcctg gactccgaca atgcgtacat gaacgtggcg gcaggcttcg acacgggctt
    15001 ctcgttctac tacgcagcgg ccaatgtcgg cggcaccatc gaggtgtgga gcggcctcaa
    15061 cggaacgggc acgatgctcg ccagcctgtc cctccctgta accggagccg gctgcgacgg
    15121 gatcaccagt ttctcctgct gggatcccat cggcgtgacc ttcgccggca ccgcaatgtc
    15181 ggtgaacttc gccggaacgg ccaaccagat cgtgttcgat aacgtgacca tcggttcggg
    15241 taccccgatc aatggcgtcc ccgaacccgc tgcgctcggg atgttcggtc tgggcgcgct
    15301 gctggtcggc ctgttcgcgg ccctgcggcg gcgtgcattg caaagctgac gacaaaaccc
    15361 cggacccttt tcttgcggcc cccgtcaacg ggggccgttt tattttctga tggatttgcg
    15421 ttccgcccat tgcgcggctg tttgaatctg tcggcagtcc ttacattttc acccttcccg
    15481 tgtggcgcag attcgctaaa tcattgaata aatatggtgc attttgctgg cacgggaaat
    15541 gctctctcga tggctcccgg gggtcgggaa tcatcaggag acgggtatga atgctctgac
    15601 gaagcggatg gggtttgcgg caatactggt ggggagcctg ttggcctgcg gcatcgcgag
    15661 tgccaccacc gtgatgacat tcgacgagtt caatccgaac ggcgagtatg tcggcaatta
    15721 ctacaacggc ggctgcggcg attcctacaa cggcggcagc gtcacctgcg gcgggccgaa
    15781 ctatggggtg acgtggaccc acgcgatcgc aggcggggcc ccgggaggac tcttcaacaa
    15841 cgttgacctc gagccgtcca gcccgggcgt gatcggcggc ttcgacgatg acaccgggtc
    15901 cttcatgaac gtggcggctg gattcgacac gggcttttcg ttctactacg cggccgcgaa
    15961 caccgcgggc agcatcaacg tgtacagcgg cctcaacggc accggcacgc tgctggcgag
    16021 cttggcgctg ccggtgaatg gctccaattg caatggctcg tttgcggcgt attcctgctg
    16081 gacaccgatc ggcgttgcct tcgacggcat cgcgcagtcg gtgaacttcg gaggcgcggc
    16141 gttctacatt gcattcgaca acgtaaccct cg
//
LOCUS       DASSNH010000022         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1115307, whole genome shotgun sequence.
ACCESSION   DASSNH010000022 DASSNH010000000
VERSION     DASSNH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115307"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VFK00_00730"
     CDS             <1..151
                     /locus_tag="VFK00_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET7591511.1"
                     /translation="FTVMFAIARTAGWIAHWIEQHEMPGGRIGRPRQVYVGATPRDYV
                     ALDKR"
     gene            complement(276..911)
                     /locus_tag="VFK00_00735"
     CDS             complement(276..911)
                     /locus_tag="VFK00_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591512.1"
                     /translation="MHKTAGYHRIHAEGRGHQLRRRIALEAARLISEHGIRDFRMAKR
                     KAAQHIGVSDEGFLPRNREIEDALREHQRLFRAGEQPHALRTRREAARQAMHFLDRFE
                     PRLVGAVLEGTADTHSPVSLHVFDDSPENVAGFLHDHGIAFETRARTLRIDRVRNAEY
                     PVLLFSADDVPMDVTVFPRDALRQAPLDPVSERPQQRASLAAVEMMLADET"
     gene            complement(1144..>2992)
                     /locus_tag="VFK00_00740"
     CDS             complement(1144..>2992)
                     /locus_tag="VFK00_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7591513.1"
                     /translation="LGQMLTHRFIDKAEYDQAIAAPDDAAPHEPPVEVDAPYLAEMVR
                     QQALQKLGNQAQTDGYVIRTTIDSRMQALANHALRSKLEEYDHRHGYRGPEAHIALPQ
                     PATPASLQKALAGYNDVSGLYPAIVTEVGDDDAKVYVQGGATIKLSAADMKWAMGFAR
                     KAKTGGKGAASILRPGDIIRVREVADEEASTAAPVKSAKDAKVAVADKQHWELQQIPA
                     AQSAMVVLSPNDGAVKALVGGFNFQLSKFNRAVQTQRQPGSSFKPFIYSAAFSRGYTP
                     ASIINDAPIALPDPSKPGGLWTPQNDDNKFRGPMRLREALAQSVNLVAIRLLDAVGVR
                     YVREYATRFGFPLDAIPPNLSMALGTASVSPMAMARAYAVFANGGFLINPYYIAEIDD
                     RDGKAIYMADPLQACRTCEARLLDEGKTTAAPAQPAAAAGDPGAMADTANALSQGESN
                     KPLLAPRVVDPRNDYLMTSLLQSVIYGGGTGAAAAKQLGRDDLAGKTGSTNDHHDGWF
                     CGFNADLVATVWVGFDDYRSLGHGEFGARTALPIWMAFMGPALQGTPEARLPMPPGIV
                     TATIDRTTGLPAPPGDGNTMTEMFKIEDLDRLRTEAKNQQNQAPAYDIF"
BASE COUNT          466 a          946 c         1033 g          547 t
ORIGIN      
        1 gttcacggtg atgttcgcga tcgcgcggac cgccggctgg atcgcgcact ggatcgaaca
       61 gcacgaaatg cccggcggcc gcatcggccg gccgcggcag gtgtacgtcg gtgccacgcc
      121 acgcgattac gtggcgctgg acaagcgttg atgaactccc ggaaagtcga ccgcttcgcg
      181 agtgggcagg gcgcgagcag ccgcgcagcg gcgtatccgg ccgcgactga aaccgcgaat
      241 ggcgggatgt gctgcgctcc tcccgccctg cgccgctagg tttcgtcggc gagcatcatt
      301 tccactgcgg ccaacgatgc gcgttgctgc gggcgttcgc tgaccggatc gagcggcgct
      361 tgccgcaagg cgtcgcgcgg aaacacggtc acgtccatcg gcacgtcgtc ggcactgaac
      421 agcagcacgg gatattccgc gttgcgcacg cggtcgatcc gcagtgtgcg ggcgcgcgtc
      481 tcgaacgcaa tgccatggtc gtgcaggaaa cctgccacgt tttcgggtga atcatcgaat
      541 acgtgcagac tcaccggcga atgggtgtcg gcggtgcctt ccagtaccgc gccgaccagc
      601 cggggttcga accgatccag gaaatgcatg gcctgccgtg cggcttcgcg acgggttcgc
      661 agcgcgtgcg gttgttcccc ggcgcggaac agccgctgat gctcgcgcaa tgcgtcctcg
      721 atctcgcgat tgcgcggcag gaaaccctcg tcactgacgc cgatgtgctg ggcggccttg
      781 cgtttggcca tccggaaatc gcggatgccg tgttcgctga tcaggcgcgc ggcttcgagg
      841 gcgatgcggc ggcgcaattg atggccgcgg ccctcggcat ggatacggtg gtacccggcg
      901 gtcttgtgca tggaaaacac cctgtagccg cgtcgggtcc agtgtcgcaa aaagcagcca
      961 caaaaagcaa aggcgtgatc aggaaccgac agccagggga caagttgcaa cgcccacgga
     1021 cgtttggggg cgcgcatcga gcccggaaat tgcaagggtg gcgctttgga aaccgcacgc
     1081 agttcgcctc cggacgcggc aacgcgagct cgaggcaaac gttttgtccc tttccggtcc
     1141 ccgtcagaaa atatcgtacg caggcgcctg gttctgctgg ttcttcgcct cggtgcgcag
     1201 tcggtcgaga tcctcgatct tgaacatctc ggtcatggtg ttgccgtcac ccgggggcgc
     1261 gggcaggccg gtggtgcgat cgatggtcgc ggtgacgatg cctggcggca tcggcagccg
     1321 cgcttcgggg gtgccctgca gcgccgggcc catgaacgcc atccagatcg gcagggcggt
     1381 gcgcgcgccg aactcgccgt gcccgagcga acgatagtcg tcgaatccca cccagaccgt
     1441 ggcgaccagg tcggcgttga agccgcagaa ccagccatca tggtggtcgt tggtggaacc
     1501 ggtcttgccg gcaaggtcat cgcggccgag ttgttttgcc gcggcggcac cggtgccgcc
     1561 gccgtagatc acgctctgca acaacgaggt catcaggtaa tcgttgcggg gatcgaccac
     1621 ccgcggtgcg agcaacggtt tgttcgactc gccctggctc aacgcgttgg cggtgtcggc
     1681 catcgcaccc ggatcgcctg ccgccgccgc cggttgtgcg ggcgccgcgg tggtcttgcc
     1741 ttcgtccagc aatcgcgctt cgcaggtgcg gcaggcttgc agcggatcgg ccatgtagat
     1801 cgccttgccg tcgcgatcgt cgatttcggc gatgtagtag ggattgatca ggaagccgcc
     1861 gttggcgaat accgcatacg cccgtgccat cgccatcggc gaaacggagg cggtgccgag
     1921 tgccatcgac aggttgggcg gaatcgcgtc cagcgggaaa ccgaagcgcg tcgcgtactc
     1981 gcgcacatag cgcacgccga ctgcgtccag caggcggatc gccaccaggt tcaccgattg
     2041 cgccagtgct tcgcgcagac gcatcggtcc gcggaacttg ttgtcgtcgt tctgcggcgt
     2101 ccacagcccg ccgggcttgg agggatcggg cagcgcgatc ggcgcgtcat tgatgatcga
     2161 agccggcgtg tagccgcgac tgaaagccgc cgaatagatg aagggcttga agctggagcc
     2221 cggctggcgc tgggtctgca ccgcgcggtt gaacttgctc aactggaaat tgaagcctcc
     2281 gaccagcgcc tttaccgcgc catcgttcgg cgagagcacc accatcgcgg actgcgcggc
     2341 ggggatctgc tggagttccc aatgctgctt gtcggcgacc gcgacctttg catccttggc
     2401 ggacttgacg ggtgccgcag tcgacgcttc ctcgtccgcc acttcgcgca cgcggatgat
     2461 gtcgcccggc cggaggatcg aggctgcgcc cttgccgccg gttttggcct tgcgcgcgaa
     2521 gcccatcgcc catttcatgt cggcggccga cagtttgatg gtcgcgccgc cctgcacgta
     2581 gaccttcgca tcgtcgtcgc ccacttcggt gacgatggcg ggatacagac cgctgacatc
     2641 gttgtagccg gccagcgcct tctgcagcga ggcaggcgtg gccggttgcg gcagtgcgat
     2701 gtgcgcctcc gggccgcgat agccgtggcg gtggtcgtat tcctcgagct tgctgcgcag
     2761 cgcgtggttc gccagcgcct gcatgcggct gtcgatggtg gtgcggatca cgtagccgtc
     2821 ggtctgcgcc tggttgccga gcttctgcag tgcctgctgg cgcaccattt cggcgagata
     2881 cggagcgtcg acctcgaccg gcggttcgtg tggtgcggcg tcgtcgggcg cggcgatcgc
     2941 ctggtcgtat tcggccttgt cgatgaaacg gtgcgtcagc atctgcccca gc
//
LOCUS       DASSNH010000023         7044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1114141, whole genome shotgun sequence.
ACCESSION   DASSNH010000023 DASSNH010000000
VERSION     DASSNH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114141"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..875)
                     /locus_tag="VFK00_00745"
     CDS             complement(390..875)
                     /locus_tag="VFK00_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7591514.1"
                     /translation="MSRIETRLAELGLSLPPAVKPPPGVVLPFRFVRVLGDRAVVSGH
                     SPQAADGSVAGPFGKVGRELTVDQGHEAARLTALSMLGSLERELGDLDRITNWVRVFG
                     MVNSAPGFDRQPAVINGCSDLILELWGDDAGAHARSAVGMAELPFGIPVEIEAEVAIR
                     R"
     gene            complement(890..1759)
                     /gene="bla"
                     /locus_tag="VFK00_00750"
     CDS             complement(890..1759)
                     /gene="bla"
                     /locus_tag="VFK00_00750"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HET7591515.1"
                     /translation="MRRRDLLKGMALGGATIAMGRMAPVAAEPGIGAARAHLAILERR
                     HGGCLGMAMLDTGSGRRITHRADEKFLMCSTFKLLLASLVLDRVDHGREKLDRRIVYG
                     KDVLLDWAPVTSKHAGAPGMTISELCAAAITLSDNTAANLLLDEVGGPQAVTAYARGL
                     GDESTLLDRIEPALNPYDTTTPNSMLGDMQQLLLGDRLSGSSRELLLGWLVACQTGLE
                     CLRAGMPDGWRIGDKTGQWDAKGVGADNDIAIAWPPDRKPVLVTAYYMNHTTDPAGRK
                     AVLAEVGRIAATM"
     gene            1918..2376
                     /gene="msrB"
                     /locus_tag="VFK00_00755"
     CDS             1918..2376
                     /gene="msrB"
                     /locus_tag="VFK00_00755"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7591516.1"
                     /translation="MPDYRKTPEALERLTPLQYQVTQEAATERPFDNEFNEHREPGLY
                     VDVVSGEPLFTSLDKFPSHCGWPSFSKPVDAANVIEKHDASHGMVRTEVRSRHGDSHL
                     GHVFDDGPRDQGGLRYCINSASLRFIPLADLEKQGYGEYRGLFDSKDKQS"
     gene            2373..2885
                     /gene="msrA"
                     /locus_tag="VFK00_00760"
     CDS             2373..2885
                     /gene="msrA"
                     /locus_tag="VFK00_00760"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7591517.1"
                     /translation="MSTERALLAGGCFWGMQDLIRKQPGVVSTRVGYSGGEVANATYR
                     NHGRHAESIEIIFDPALISYRKLLEFFFQIHDPTTTNRQGNDIGTSYRSAIFYTNDEQ
                     KRVAEDTIADVEASGLWPGKVVTEVVPAGDFWEAEPEHQDYLERYPDGYTCHFVRPDW
                     KLPVRETSAG"
     gene            3038..4111
                     /locus_tag="VFK00_00765"
     CDS             3038..4111
                     /locus_tag="VFK00_00765"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7591518.1"
                     /translation="MTQTPAYATQAADKPLGPQAIERREPGPRDVAIDIRYCGVCHSD
                     LHTARNEWGNTAYPCVPGHEIVGIVTAVGKDVQGFKAGDTVGVGCMVDSCGHCDSCKA
                     GEENYCENGFTGTYNGPTQEGHGNTYGGYSGQIVVDEKFVLHIRHEDNLAAVAPLLCA
                     GITTYSPLRHWNIGPGKKVGVVGLGGLGHMAVKLAHAMGAHVVLFTTSPGKIEDGKRL
                     GADEVVISKDAAQMAAHAASFDFILNTVAAPHNLDAYLNLLKRDGTMCLVGAPPIDSP
                     HPSPTVFNLILKRRGIAGSLIGGIRETQEMLDFCAEHGIVCDIEMIAMRDINEAYERM
                     LRSDVKYRFVIDMQTLKDEAQAA"
     gene            4245..4667
                     /locus_tag="VFK00_00770"
     CDS             4245..4667
                     /locus_tag="VFK00_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7591519.1"
                     /translation="MHELPPFHLAFPVASLGEARAFYGGLLGCPEGRSSDAWVDFDFH
                     GHQIVAHLAPEELRAPAAHDVDGDAVPVRHFGVVLSMADWQALADKLREAGTHFVVEP
                     HVRFRGQPGEQATMFLLDPSGNALEFKAFADRSRLFAR"
     gene            complement(4773..5012)
                     /locus_tag="VFK00_00775"
     CDS             complement(4773..5012)
                     /locus_tag="VFK00_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591520.1"
                     /translation="MANKVQGEGDYESARNYRKETERSAREQTKDGKQVKGDAGQASD
                     KLTPEEREGRSRAKEPRQDKRDAEYMDELERKKKH"
     gene            5197..5985
                     /gene="ygiD"
                     /locus_tag="VFK00_00780"
     CDS             5197..5985
                     /gene="ygiD"
                     /locus_tag="VFK00_00780"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HET7591521.1"
                     /translation="MPKLLPALFVGHGNPMNAMSSNPWTEAWRSLGERIGKPRAILAI
                     SAHWYAPGTAVTANQAPRTIHDFGGFPEELYRVQYPAPGDPELARGVQAMLAPLPVEM
                     DANWGLDHGTWSVLRHMYPDSDVPVVQLRIDESQPAAFHHEIGRQLAALRSEDVLVFG
                     SGNLVHNLHTYSWGRHQPAPFEWAVRFEARAREWMLGGDHAPLIDYEAFGRDALLSIP
                     TPEHYLPLLYVLGARESHETVSFPVEGVDGGSISMLAVQVGELP"
     gene            6042..7031
                     /locus_tag="VFK00_00785"
     CDS             6042..7031
                     /locus_tag="VFK00_00785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7591522.1"
                     /translation="MIPVSILDLAPIVEGGDAAQSFRNTVDLAQTAERLGYRRYWLAE
                     HHNMPGIASAATSVLIGHVAGATSTIRVGAGGIMLPNHSPLQVAEQFGTLATLYPQRI
                     DLGLGRAPGTDQPTARALRRYFDSADDYPQNVVELLGYFRPAVAGQAVQAVPGAGVDV
                     SVWMLGSSLFSAQLAGHLGLPYAFASHFAPAAMEQALQQYRALFRPSAQLARPCAMLG
                     LNVVAAESDAEARRLFTSQQQMFINLRRGRPGRVPPPIDAIEDFAEPAELDRIGQALS
                     CAVIGGPETVRAGIEAFVATHRADELIVTANTFDHAARRRSFEIVAEVLGLRG"
BASE COUNT         1221 a         2462 c         2206 g         1155 t
ORIGIN      
        1 accccatcgt cgccgccaac caggacagca cacgcagagc aaatcgaaac gaccccacgc
       61 caacgatagc acaacccgtt tgcatgggcg caagcactgc ccgaaaaccg gcgaagggag
      121 atcccccact ctcgggagat cccattcgag gcgtcaccgg gaatggggct tgtatcggcc
      181 cttgcgcctt gattcgtgat catgctggac gtcgaaacga cgcccgtgga aaggccgctg
      241 gaactgctga ccatgcaatc ggcccgccgc ctttttcgcg ccgtatccgg cggctcgcct
      301 ttcggccatc ggcttcgccg attttcgctt cggcatgcgt ggcttcccgg tcccgctttc
      361 gccggaatgg cgatccaggt cagagctccc tagcggcgga tagccacttc cgcctctatc
      421 tccaccggaa tgccgaaggg cagctcggcc atgccgaccg cgctgcgcgc gtgcgcgccg
      481 gcgtcatcgc cccacaactc caggatcaga tcggagcagc cgttgatcac cgccggttgc
      541 ctgtcgaagc cgggtgccga attgaccatg ccgaacacgc gaacccagtt cgtgatccga
      601 tccaggtcgc cgagttcgcg ctcgaggctg cccagcatcg acagtgcggt gagccgcgca
      661 gcttcatggc cttggtccac ggtgagctcg cgccctacct tgccgaaggg tccggcgacg
      721 gaaccgtccg ccgcctgcgg gctgtgcccg gataccacgg cccgatcgcc gagcacgcgt
      781 acgaaacgga agggcaagac cacgccgggc ggcggcttga ccgccggcgg cagcgacagg
      841 ccgagttccg cgagtcgggt ttctatcctg gacatctggt ttcccccatt tacatcgtcg
      901 ccgcgatccg gcctacttcc gccagcaccg ccttgcggcc ggccgggtcc gtcgtgtggt
      961 tcatgtagta cgcggtgacc agaaccggct ttcggtcagg cggccaggca atcgcaatgt
     1021 cgttgtcggc gccgacgccc ttcgcgtccc actgcccggt cttgtctccg atccgccatc
     1081 cgtccggcat cccggcgcgc aagcactcga gtccggtttg gcaggcgacc agccagccca
     1141 gcaacaactc gcgcgaggac cctgaaaggc gatcgccaag cagcaactgc tgcatgtcac
     1201 cgagcatcga attcggggtg gtcgtgtcat agggattcag cgcgggttcg atgcgatcca
     1261 gcagcgtcga ttcgtcgcca agcccgcggg catacgcagt gaccgcctgc gggccgccga
     1321 cttcgtccag cagcaggttg gctgccgtgt tgtcgctcaa cgtgatcgcg gccgcgcata
     1381 gttcggaaat cgtcatgccg ggagctcccg cgtgtttgct agtcacgggc gcccagtcga
     1441 gcagcacgtc cttcccgtaa acaatgcgcc gatcgagttt ctccctgccg tggtccacgc
     1501 gatccagcac cagcgacgcc agcagcagct tgaaagtgct gcacatcagg aacttttcgt
     1561 cggcccggtg cgtgatgcgg cgaccgctgc ctgtatccag catcgccatg ccgaggcagc
     1621 cgccatgccg ccgttccagt atcgccagat gcgccctcgc ggccccgatt cccggctcgg
     1681 ccgcaacagg cgccatgcgg cccatcgcga tggtcgcgcc acccaatgcc attcccttca
     1741 gcaggtcgcg gcgccgcatg cgttgttccc aacgatcagg tgatgttgca gcttacgggg
     1801 aaaccgcaca gcgcggacat gacgtccagg cagcgggcag gaaccttgag gccgcccggc
     1861 ggcgcctcca tgtgcttgga atcgccgttt ccgacgccga tccggcagga ggatccgatg
     1921 ccagactacc gcaagacccc cgaagccctg gagcgcctga ctccgctgca ataccaggtg
     1981 acccaggagg cggcgaccga acgaccgttc gacaacgaat tcaacgagca tcgcgagccc
     2041 ggactgtacg tggacgtggt gtcgggcgaa ccgctgttca cttccctgga caagttcccg
     2101 agccactgcg ggtggcccag cttcagcaag ccggtggatg ccgccaacgt gatcgagaag
     2161 cacgacgcga gccacggcat ggtccgcacg gaagtgcgct ctagacatgg cgacagccac
     2221 ctcggccacg tgttcgacga cggcccccgc gaccagggcg gattacgcta ctgcatcaac
     2281 tccgcatcac tacgcttcat accgctcgcc gatctggaga aacagggcta cggcgagtat
     2341 cgtggactgt tcgacagcaa ggacaagcaa tcatgagcac tgaacgcgcg ctgctcgccg
     2401 gcggatgctt ctggggcatg caggacctga tccgcaagca gcccggcgtg gtttccacgc
     2461 gggtcggcta ctcgggcggg gaagtcgcca atgcgacgta ccgcaaccac ggaaggcacg
     2521 ccgaatccat cgaaatcatc ttcgatccgg cgctcatcag ctaccgcaag ctgctggaat
     2581 tcttcttcca gatccacgac ccgacgacga ccaaccgcca gggcaacgac atcggcacga
     2641 gttaccgctc ggcgatcttc tacacgaacg acgaacaaaa gcgcgtcgcc gaagacacca
     2701 tcgccgatgt ggaggcgtcc ggcttgtggc ccggcaaggt cgtcaccgaa gtcgtgccgg
     2761 ccggcgactt ctgggaggcc gaacccgagc accaggatta cctcgaacgc tatccggacg
     2821 gctacacttg ccatttcgtg cgccccgact ggaagctgcc ggtgcgggaa acgagcgctg
     2881 gctagcctgg aacgttgacc ggttcgaggc cgaaggccgt aaccggcaat cgccgcgcca
     2941 agccgatgcg ctccgtcgct tcgctcctcg agccggccta tgcggacttt tcccctcccc
     3001 tgccagggga gggttcgtct tttgccagga gacaggaatg acccaaaccc ccgcctacgc
     3061 cacccaggcc gccgacaagc cgctcggccc ccaggcaatc gaacggcgcg agcccggacc
     3121 gcgcgacgtc gccatcgaca tccgttattg cggcgtctgc cactccgacc tgcacaccgc
     3181 gcgcaacgag tggggcaaca cggcttatcc ctgcgtgccg gggcacgaaa tcgtaggcat
     3241 cgtgacggcg gtcggcaagg acgtgcaggg cttcaaggcc ggcgacaccg tcggcgtcgg
     3301 ttgcatggtc gacagttgcg ggcattgcga ttcctgcaag gccggcgagg aaaactactg
     3361 cgagaacgga ttcaccggca cctacaacgg ccccacgcag gagggtcacg gcaataccta
     3421 cggcggctat tccggccaga tcgtcgtcga cgaaaaattc gtgctgcaca tcaggcacga
     3481 ggacaacctc gccgcggtcg cgcccctgct gtgtgccggc atcaccacct attcgccgct
     3541 gcggcactgg aacatcggac cgggcaagaa ggtcggcgtc gtgggcctcg gcggcctcgg
     3601 ccacatggcc gtcaagctcg cccacgcaat gggcgcacac gtggtgctgt tcaccacctc
     3661 gccggggaag atcgaagacg gcaagcgcct tggcgcggac gaagtggtga tctcgaaaga
     3721 cgccgcacaa atggccgcgc acgccgcgag tttcgacttc attctcaaca ccgtggctgc
     3781 accgcacaat ctcgacgctt acctcaacct gctgaagcgt gacggcacca tgtgcctggt
     3841 cggagcgccg ccgatcgatt caccgcaccc gtcgcccacc gtgttcaatc tgatcctgaa
     3901 gcgccgcggc atcgccggtt cgctgatcgg cggcatccgc gaaacccagg aaatgctgga
     3961 tttctgtgcg gagcacggga tcgtctgcga catcgaaatg atcgccatgc gcgacatcaa
     4021 cgaagcctac gagcgcatgc tgaggagcga cgtgaaatac cggttcgtga tcgacatgca
     4081 gacgctgaag gacgaggcgc aggcggcgta accggcacga tcgatctgcc ctggatgcgg
     4141 gcggggcgcg atgatccacg gacagcgcgc ttcgacctgg tatgcggctc gggccacgct
     4201 cggggcaccg aacaacttcc ggccttcctg cggagacaac cgccatgcac gaactcccgc
     4261 ccttccatct cgcgtttccg gtcgcctcgc tcggcgaagc ccgggcgttc tatggtggac
     4321 tgctcggctg cccggaaggc cggtccagcg acgcgtgggt ggacttcgat ttccacggcc
     4381 accagatcgt cgcgcacctc gcgcccgaag agctgcgggc gccggccgcg cacgacgtgg
     4441 acggcgacgc cgtgccggtg cggcatttcg gggtggtgtt gtcgatggcc gactggcagg
     4501 cgctcgccga caagctgcgg gaagcgggga cccacttcgt ggtcgaaccg cacgtgcgct
     4561 tcaggggcca acccggcgaa caagccacga tgttcctgct ggacccgtcg ggcaacgcgc
     4621 tcgagttcaa ggcgttcgcc gatcggtcgc ggctgtttgc gcgctgatcg cggatcctcg
     4681 cgggcacgcc actctccgca ggcaagaagc ggcgcgcagg gcacgggccc ggcgcgccga
     4741 catgccacga cgcgagctgc cgcaagggtc gcttagtgct tctttttcct ttccaactcg
     4801 tccatgtact cggcgtcgcg cttgtcctgg cgcggctcct tggcgcgtga gcggccttcg
     4861 cgttcctccg gcgtcagctt gtcgctcgcc tggccagcgt cgcccttgac ctgcttgccg
     4921 tccttggtct gctccctggc ggaacgttcc gtctccttgc gatagttccg cgcgctttcg
     4981 taatcgcctt cgccctgtac cttgttggcc atgtcgtgcc tccttcgctg gaacgtggca
     5041 tccgtcgcgc cacgcgagca tcatgtctag caggcgcggc ctgaacgccc gcgcatcgag
     5101 gcactgcgcg atcgcttgac ggttccggca agcatcgacc ggtccgcccg ggacgcaagc
     5161 cggggcggtt tgaccttcca agacaggccc accgccatgc ccaaactcct gcccgccctc
     5221 ttcgtcggcc acggcaaccc gatgaatgcg atgagtagca acccctggac cgaagcctgg
     5281 cgcagcctcg gcgagcgaat cggaaagccg cgtgcgatcc tcgccatttc tgcgcactgg
     5341 tacgcacccg ggaccgcggt caccgccaac caggcgccgc ggaccatcca cgacttcggc
     5401 gggtttccgg aagaactcta ccgagtgcag tacccggcac ccggcgatcc ggaactcgcc
     5461 cgcggcgtgc aagcgatgct ggcgccgctg ccggtcgaaa tggatgcgaa ctggggcctc
     5521 gaccacggca cctggtcggt gctgcggcac atgtacccgg actcggacgt gccggtggtg
     5581 caactgcgca tcgacgaaag ccagcccgcc gcgttccatc acgaaatcgg ccggcagctc
     5641 gccgcattgc gctcggaaga cgtgctggtc ttcggcagcg gcaacctggt acacaacctg
     5701 cacacctatt cctgggggcg ccaccagcct gcgccattcg agtgggcggt acgcttcgaa
     5761 gcccgggcac gcgaatggat gctcggcggt gaccacgcgc cgttgatcga ctacgaagcc
     5821 ttcggccggg atgccctgct ctcgatcccg acgccggaac attacctgcc actgctgtac
     5881 gtgctcggcg cgcgcgagtc ccacgaaacg gtcagcttcc cggtcgaagg cgtcgacggc
     5941 ggatcgatct cgatgctggc ggtgcaggtt ggcgaactgc cgtgacgacc ccacatcggt
     6001 atgccgtgca ttgaaaaatc cgtggcagcc gagtccccga catgattcca gtttcgattc
     6061 tcgacctcgc gccgatcgtg gagggcggcg acgccgcgca atccttccgc aacaccgtgg
     6121 acctcgcgca aaccgcggag cgcctgggct accggcgcta ctggctggcc gagcaccaca
     6181 acatgccggg catcgccagt gccgcgacgt cggtcctgat cggccacgtg gccggcgcca
     6241 cctcgaccat ccgggtcggg gcgggcggca tcatgctgcc caaccattcg ccgctgcagg
     6301 tggccgagca gttcgggaca ctggccaccc tgtatccgca acgcatcgac ctcgggctcg
     6361 ggcgcgcgcc gggcaccgac cagccgaccg cgcgcgccct gcggcgctac ttcgacagcg
     6421 ccgacgacta cccgcagaac gtcgtggaac tgttgggcta cttccgaccg gctgtcgcgg
     6481 ggcaagccgt gcaggcggtt ccgggcgcag gcgtcgacgt gtcggtatgg atgctgggct
     6541 ccagcctttt cagtgcccag ctcgccggcc acctcgggct gccgtacgcg ttcgcgtcgc
     6601 acttcgcgcc cgcagcgatg gagcaggcgc tacagcaata ccgggccctg ttccgacctt
     6661 ccgcccaact tgcgcggccc tgcgcgatgc tgggcctgaa tgtcgtcgcc gccgaatccg
     6721 atgccgaggc acgccggctg ttcaccagcc agcagcagat gttcatcaac ctgcgccgcg
     6781 gacggcccgg ccgggtgccg ccaccgatcg acgccatcga ggacttcgcg gagcccgccg
     6841 agctggaccg gatcgggcag gcgctttcat gcgcggtcat cggtggcccc gaaacggtgc
     6901 gcgccggcat cgaagccttc gtcgccaccc accgggcgga cgagttgatc gtgaccgcca
     6961 acaccttcga ccatgccgca cggcgccgct cgttcgagat cgtggccgag gtgctgggcc
     7021 tgcgcggctg acgcagccgg aagg
//
LOCUS       DASSNH010000024         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1869713, whole genome shotgun sequence.
ACCESSION   DASSNH010000024 DASSNH010000000
VERSION     DASSNH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869713"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFK00_00790"
     CDS             complement(<1..857)
                     /locus_tag="VFK00_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET7591523.1"
                     /translation="MRSTRFLILAALAAIGLPAQAITIDGRIDPAEWQDARHVTDFRQ
                     TQPLTGKPAEHPVEAWILATPDGLAVAMRVTQPPNVRRTRQQVQRDFHDPVDRVNVEI
                     DFNGDGRTGYDFSVASTGAVDDEVITNENQFDADWDGIWQHAVTQDAEGWSVEVLIPW
                     YTAPMHAASGGTREIGIYLDRVFATTGERVSWPAVHYQQPRYLSAFERIEVPAYSQSL
                     LAVTPYVSSRYDNVGHDGGHSEGVDVFWKPNGQFQLTATLNPDFGQVESDDLVINFGA
                     TETYFSDKRP"
     gene            complement(939..1259)
                     /locus_tag="VFK00_00795"
     CDS             complement(939..1259)
                     /locus_tag="VFK00_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591524.1"
                     /translation="MARFVALLLIGPWLLVLGWLYWLYVRRRVANGVSVRFDVGVLAI
                     AALATIASTAFGYQAALGHGGPIWKQVAAALGAYAGFNVVVLFGLLRHWLARGHMTSA
                     PRGS"
     gene            complement(1262..2641)
                     /locus_tag="VFK00_00800"
     CDS             complement(1262..2641)
                     /locus_tag="VFK00_00800"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7591525.1"
                     /translation="MNQRILTGITTTGTPHLGNYVGAIRPAIAASRDADTDAFFFLAD
                     YHALIKCDDPARIARSRLEIAATWLACGLDPQRVTFYRQSDIPEIPELTWMLTCVTAK
                     GLLNRAHAYKAAVDQNTAKGEDPDAAINAGLYMYPVLMAADILIFDANKVPVGRDQVQ
                     HIEMARDIAQRFNHIHGGAWRAVGGQDEPFVLPEPAIEESVATLPGLDGRKMSKSYDN
                     TVPLFEGGAKALREAVARIVTDSREPGEPKDPDSSSLFAIFRAFAGDEESATFRSALE
                     TGLGWGEAKQKLVERIEVEIAPLRERYDALMARPDAIEDALREGAKKARAIARPRLET
                     MRRAVGLGALGTPAGKTKASGKADKRARIVSFRDGEGFHFRLVGADGEELLLSRVFAD
                     PRSAGQASRALSAEDADSSLKPIDASRFAYMQDGESLATSAEFPELAARDAAIERTRA
                     ALASLRDAE"
     gene            complement(2875..3927)
                     /gene="hemE"
                     /locus_tag="VFK00_00805"
     CDS             complement(2875..3927)
                     /gene="hemE"
                     /locus_tag="VFK00_00805"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7591526.1"
                     /translation="MAEVSNRSNRFLRALHRQPVDTTPVWIMRQAGRYLPEYRATRAR
                     AGSFLALAQTPELACEITLQPVERFELDAAILFSDILTVPDAMGLGLHFVEGEGPKFH
                     HPLRNAADIAKLGVPDPESSLRYVTDAVRLIRSELGGRLPLIGFAGSPWTLACYMIEG
                     EGSRDFVRVKAFALDEPTAMHSLLDVNARAVAAYLAAQAAAGAQALMVFDSWGGMLAP
                     AMFLEFSQRYLAQIASLLRDDSHARDVPLILFSKGANASLEALADTGCAALGVDWTIT
                     LDEARRRVGNRVALQGNLDPATLLASPEVIRREAKRVLDDFGPHPGHVFNLGHGITPD
                     VDPEHVAVLVDAVHAA"
     gene            complement(3920..4177)
                     /locus_tag="VFK00_00810"
     CDS             complement(3920..4177)
                     /locus_tag="VFK00_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HET7591527.1"
                     /translation="MHLYMQTPPGNGAAPRYCRIALEQDLLGGWTLYRESGTEGGRAT
                     LKREVYLDRDTALAAFESARDSQLKRGFKVMITQGVDAPHG"
     gene            complement(4343..5449)
                     /gene="aroB"
                     /locus_tag="VFK00_00815"
     CDS             complement(4343..5449)
                     /gene="aroB"
                     /locus_tag="VFK00_00815"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7591528.1"
                     /translation="MTTSVEVELPGSRYPIRIGSGLLADGPAWCDSIRGVHALVVSDE
                     NVAPLYADRLRDTLARAHGTTVRMATLVLPAGEAHKNLDAVAHVLDALARLGATRDAC
                     VLALGGGVVGDIAGFAAACWMRGIDFVQFPTTLLAMVDASVGGKTGVDHPAGKNLIGA
                     FHQPRAVVADLGTLATLPDRELRAGFAEVIKVASIGDADFFAWLEDHADALLQRDAEA
                     LGHAIATGCRFKAGVVARDEREAGERALLNFGHTFGHALEAEAGYGTLLHGEAVAIGM
                     VLAARLSERLGMAGHGDAERLRGLLARIGLPTNPPPGLSAEALLARMRLDKKNRAGSL
                     RLILWRGIGRAELVEGVDIAALRAVITAPSAPSR"
     gene            complement(5446..>5867)
                     /locus_tag="VFK00_00820"
     CDS             complement(5446..>5867)
                     /locus_tag="VFK00_00820"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7591529.1"
                     /translation="SVATIFEIEGEEGFRRRESDLLADLTARSGIVLATGGGAVLVAA
                     NRRLLRERGFVVWLETGVEQQMQRLQRDRKRPLLDAPDRRERLEQLAQLRDPLYREIA
                     DFTVQSGSGESCHNAAARVATLLEQHWQRLPATRATA"
BASE COUNT          927 a         1950 c         2033 g          957 t
ORIGIN      
        1 gggcgcttgt cgctgaagta cgtttcggtc gcgccaaagt tgatcaccag gtcgtcgctt
       61 tccacctggc cgaagtcggg gttcagcgtc gccgtcaact ggaactggcc gttcggcttc
      121 cagaacacgt ccacgccttc actgtgtccg ccgtcgtggc cgacgttgtc gtaacgggac
      181 gagacatacg gcgtcaccgc cagcagcgac tggctgtagg cgggtacctc gatgcgctcg
      241 aatgcggaga gatagcgcgg ctgctggtaa tgcaccgcgg gccacgacac acgctcgccg
      301 gtggtggcga agacccggtc gaggtaaatg ccgatttcac gggtaccacc gctggccgca
      361 tgcatgggcg ccgtgtacca ggggatcagg acctccaccg accagccttc ggcgtcctgg
      421 gtgacggcgt gttgccagat gccgtcccag tcggcgtcga actggttttc gttggtgatc
      481 acctcgtcgt cgaccgcacc ggtggacgca acggagaagt cgtagccggt gcggccatcg
      541 ccattgaagt cgatctccac gttgacgcga tcgaccggat cgtggaagtc gcgctgcacc
      601 tgctgtcgcg tgcgcctgac gttcggcggt tgggtgacgc gcatcgccac cgccaatccg
      661 tcgggggtgg cgaggatcca cgcttcgacc ggatgttccg ccggcttccc ggtcaacggt
      721 tgggtctggc ggaaatcggt gacgtgacgc gcgtcctgcc actcggccgg atcgatgcgg
      781 ccgtcgatgg tgatggcctg ggctggcaaa ccgatcgccg cgagtgcggc caatatcagg
      841 aaccgggtgg aacgcattca tccaatccgt ggcggcgggt cgcaatcggg cagcggaacc
      901 cggagcgcgt catgccgcgc gcaagctaac ggcgccggct aggaaccgcg aggggcggaa
      961 gtcatgtgcc cgcgcgccag ccagtgccgc agcaggccga acagcacgac cacgttgaaa
     1021 cccgcgtatg cgccgagcgc cgccgcgacc tgcttccaga tcgggccgcc gtggccaagc
     1081 gccgcctgat agccgaacgc ggtgctcgcg atggtggcca gcgccgcgat cgccagcacg
     1141 ccgacatcga agcgcacgga aacgccgttc gccacgcggc ggcggacgta aagccagtag
     1201 agccagccca gcaccagcaa ccagggtccg atcagcagca gtgcgacgaa acgggccatg
     1261 cctattcggc gtcgcgcaac gaggccagcg ccgcgcgggt gcgttcgatt gcggcgtcgc
     1321 gcgcggccag ctcgggaaat tccgcgcttg tcgcaaggct ctcgccgtcc tgcatgtacg
     1381 cgaatcgcga tgcatcgatg ggtttgagcg agctgtccgc atcctcggcg gaaagggcgc
     1441 gggatgcctg tcccgcgctg cgtggatcgg caaagacgcg cgacaacagc aactcctcgc
     1501 cgtcggcacc gaccaggcgg aaatggaatc cctcgccatc gcggaagctg acgatgcgcg
     1561 cgcgcttgtc tgccttgccc gacgccttgg tcttgcccgc aggcgtgccc agcgcgccca
     1621 gccccaccgc gcgccgcatg gtctcgagcc gcggccgggc gatcgcgcgc gctttcttcg
     1681 cgccttcgcg cagcgcgtcc tcgatggcgt cgggccgcgc catcaacgcg tcgtagcgct
     1741 cacgcagggg cgcgatctcc acttcgatgc gctcgaccag cttctgcttg gcctcgcccc
     1801 agccaagacc ggtttcgagc gcgctgcgga aggtggcgct ttcttcgtca cccgcaaacg
     1861 cccgaaagat cgcgaacagg ctcgaggagt cgggatcctt gggctcgccg ggttcgcgcg
     1921 aatcggtgac gatgcgcgcc accgcctccc gcaaggcctt cgcgccgccc tcgaacagcg
     1981 gcacggtgtt gtcgtaactc ttcgacatct tgcggccgtc gaggccgggc agggtggcaa
     2041 ccgattcctc gatggcaggc tcgggcaaga cgaacggctc gtcctggccg ccgaccgcgc
     2101 gccacgcgcc gccatggatg tggttgaagc gctgcgcgat gtcgcgcgcc atctcgatgt
     2161 gctgcacctg gtcgcggccg accggcacct tgttggcgtc gaagatcagg atgtccgcag
     2221 ccatcagcac cggatacatg tagaggcccg cgttgatcgc ggcgtccggg tcctcgccct
     2281 tcgcggtgtt ctggtccacg gcggccttgt acgcgtgcgc gcggttgagc aaacccttgg
     2341 cggtcacgca agtgagcatc caggtcagtt ccgggatctc cgggatgtcc gattgccggt
     2401 agaacgtgac gcgctgcgga tcgagcccgc aggccagcca tgtcgcggcg atctcgaggc
     2461 gcgagcgcgc gatgcgcgcg ggatcgtcgc acttgatcag cgcgtggtaa tcggcgagga
     2521 agaaaaacgc atcggtgtcg gcatcgcgac tggctgcgat cgccggccga atcgcaccga
     2581 catagttgcc gagatgcggc gtgccggtgg tggtgatgcc ggtgaggatg cgctggttca
     2641 tggctcgcgg tgcgggcaag gcaagaccgc gattttaggc gaattgcgcg tggctgtccc
     2701 ggacggggtc gcgttttgcc gccatctgcg gcgcgaaaca cttgctgcgg taaacaactc
     2761 tcgatgcgcg atcaacggga aacgggtagt ccggatggag ccgcgcagcg gcgcaatccg
     2821 gggcatgccc gatacccccg gattccgctt tgctccatcc gggctacggc ggcgtcacgc
     2881 ggcgtgcact gcgtccacca ggaccgcgac atgttccgga tccacgtccg gcgtaatgcc
     2941 gtggccgaga ttgaatacgt ggcccggatg tggtccgaag tcgtcgagta cccgtttggc
     3001 ctcgcgccgg atcacctcgg gcgaagccag cagcgtcgcg ggatcgaggt tgccctgcag
     3061 cgcgacacga ttgccgacgc ggcggcgggc ttcatcgagg gtgatcgtcc agtccacgcc
     3121 gagcgccgcg cagccggtgt ccgccagtgc ttcgaggctg gcgttcgcgc ccttggagaa
     3181 caggatcaac ggcacgtcgc gcgcgtggga atcatcgcgc agcagcgatg cgatctgcgc
     3241 gaggtagcgt tgcgagaatt cgaggaacat cgccggcgcc agcatgccgc cccagctgtc
     3301 gaacaccatc agcgcctgcg cgcccgcggc ggcttgcgcg gcgaggtagg ccgctaccgc
     3361 gcgcgcgttc acgtccaaca gcgagtgcat cgccgtgggt tcgtccagcg cgaatgcctt
     3421 gacacgcacg aagtcacgcg agccttcgcc ttcgatcatg tagcaggcga gcgtccatgg
     3481 gctgcccgcg aagccgatca gcggcaaccg gccgccgagt tcgctgcgga tcaggcgcac
     3541 cgcatcggtg acgtagcgca gcgaggattc cggatcggga acgccgagct tcgcgatgtc
     3601 cgcggcgttt cgcaacggat gatggaactt cggaccttcg ccctcgacga aatgcaggcc
     3661 gaggcccatg gcgtcgggca cggtgaggat gtcggagaac aggatcgcgg catccagttc
     3721 gaaacgttcg accggttgca gggtgatttc gcaggcgagc tccggcgtct gcgccagcgc
     3781 caggaaactg cccgcgcgcg cgcgggtcgc ccggtactcc ggcaggtagc ggccggcctg
     3841 gcgcatgatc cagacgggcg tggtgtcgac cggttggcgg tgcaatgcgc gcaggaaccg
     3901 gttgctgcgg ttggacacct cagccatgcg gtgcatccac gccctgggtg atcatcactt
     3961 tgaagccgcg cttcaactgg ctgtcgcgcg cgctttcgaa ggcggcgagc gcggtgtcgc
     4021 ggtcgaggta aacctcgcgc ttcagggtgg cgcgtccccc ctcggtcccg gattcgcggt
     4081 acagcgtcca gccgccgagc aggtcctgct ccagcgcgat gcggcaatag cgcggtgcgg
     4141 cgccgttgcc gggcggggtt tgcatgtaaa gatgcatttg cgaattgtat gggagaagcg
     4201 cgatggcatc cccacgtcgc cggcgtgcat ccccccgccg ctgcgcggcg accccttcgc
     4261 gagcgaagcg ggtgatagcg gcatcggtgc aggtgttttc ttccccgctt ccatgggaag
     4321 ggggatcgag ggggatgggt tttcagcgcg acggagcgct gggcgcagtg atcaccgcgc
     4381 gcaaagcagc gatatcgacg ccttccacga gttcggcgcg cccgatcccg cgccacagga
     4441 tcaatcgcag cgaacccgcg cgattcttct tgtcgaggcg catgcgcgcg agcagcgcct
     4501 ctgcggagag tcccggtggt ggattggtcg ggaggccgat ccgcgccagc agtccgcgga
     4561 gcctttccgc atcgccgtgc cctgccatgc cgagccgctc cgacagccgc gccgcgagca
     4621 ccatcccgat cgcgacggct tcgccgtgca gcaacgtgcc gtagccggct tccgcttcca
     4681 gcgcgtggcc gaaggtgtgg ccgaagttga gcagggcgcg ttcgccggcc tcgcgctcgt
     4741 cgcgcgccac gacgcccgcc ttgaagcggc agccggtggc gatggcgtgg ccgagtgcct
     4801 cggcgtcacg ttgcaacaac gcgtccgcat ggtcttcgag ccaagcgaag aaatcggcat
     4861 cgccgatgga ggcgaccttg atcacttccg cgaatccggc gcgcaattcg cgatccggca
     4921 gggtcgcgag tgtgccgagg tcggcgacca ccgcgcgcgg ctggtggaac gcgccgatca
     4981 ggttcttgcc ggcggggtgg tcgacgccgg tcttcccacc gaccgaggcg tcgaccatcg
     5041 cgagcagcgt ggtcgggaac tgcacgaaat cgatgccgcg catccagcag gcggcggcga
     5101 aaccggcgat gtcgccgacc acgccgccgc cgagcgcgag cacgcaggcg tcgcgggtcg
     5161 ctccgagccg cgccagtgca tccagtacgt gggcgaccgc atcgaggttc ttgtgcgctt
     5221 cacccgctgg cagtaccagt gttgccatcc gcaccgtggt gccgtgcgcc cgcgccaggg
     5281 tgtcgcgcag ccggtccgca tagagcggcg ccacgttttc gtcgctgacg accagcgcgt
     5341 gcacaccgcg gatcgaatcg caccaagccg gaccgtccgc gagcaatccg ctcccgatgc
     5401 ggatcgggta tcggctgccg ggcagctcca cctcgacgct ggtcgtcatg cggttgccct
     5461 ggtcgcgggc agacgctgcc agtgttgctc cagcagcgtg gcgacgcgcg ccgcggcgtt
     5521 gtggcaggac tcgccactgc cgctctgcac ggtgaagtcg gcgatttcgc ggtagagcgg
     5581 atcgcgcaac tgggcgagct gttccagccg ttcgcggcga tcgggcgcat ccagcagcgg
     5641 ccgcttgcga tcgcgttgca gacgctgcat ctgctgttcg acgccggttt ccagccagac
     5701 cacgaagccg cgctcgcgca gcagccggcg attggccgcg accagcacgg cacctccgcc
     5761 ggtggcgagc acgatgcccg aacgcgcggt gaggtcggcc agcaagtcgg attcgcggcg
     5821 gcggaatcct tcctcgcctt cgatctcgaa gatcgtcgcg acgctca
//
LOCUS       DASSNH010000025        13603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1318723, whole genome shotgun sequence.
ACCESSION   DASSNH010000025 DASSNH010000000
VERSION     DASSNH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 13603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13603
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318723"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1936
                     /locus_tag="VFK00_00825"
     CDS             74..1936
                     /locus_tag="VFK00_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7591530.1"
                     /translation="MQPTDIRNPQYFHKVVDCQWACPAHTQVPRYIRLIAAGRYDDAY
                     LLNWDSNVFPGILGRTCDRPCEPACRRGRVEENNGPPQDKTKPEPVAICRLKRVAADN
                     RSDGIETRMRERLPEPVPGADRFRVACIGAGPASLALARDLAPLGYRVTIFEADAKGG
                     GFMRTQVPRFRLPEVVIDEEVGYILDLGVNFVGNRRIDSLRKVLDEGFDAIFVGCGAP
                     RGRDLHIPGRDENTVKIHVGIEWLASVYFGHVTKVGRRVLVLGGGNTAMDCCRSARRL
                     GGDDVKVIVRSGYDEMKASPWEKEDAEHEGVEILNWLNPKSFVHENGKLTGMWFTPMR
                     AERDANGRRKLLPTGEPDRLFPCDEVLIAVGQENAFPWIERDLCLEFDEWGTPKLDKV
                     TMQSTLPRVFFGGDAAFGPKNIIWAVEHGHQAAISIHNFLQGTDLRERPSPLTNLVSQ
                     KMGIHEWSYDNDISPDVRYVVPWAPKEKTLTNIHQEVELGFDAQTAWKEAQRCLNCDV
                     QTVFSKDLCIECDACVDVCPMDCITFTADGEEAELRTRLTAPANNTGQDLYVSGPVKT
                     GRIMAKDEDVCLHCGLCAERCPTGAWDMRQFLFAESYAARGEDYSPRPAKKVAA"
     gene            2112..3950
                     /locus_tag="VFK00_00830"
     CDS             2112..3950
                     /locus_tag="VFK00_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET7591531.1"
                     /translation="MTAITATNDFVVKFANVNGSGSASANELFAKCILRMGVPVSPRN
                     IFPSNIQGLPTWYEVRVSEAGWLGRRGGYDLLVAMNPQTWDADVAGLSPGGYLFHDNS
                     KPLAPAKFRDDVTVIGVPLTDIANRTWDDPRQRQLFKNIMYLGALSVLLGIERDVIVQ
                     LVGEQYKGKQALLEPNIRALDLGRDYAQHNLPPVGLKIERRDNVGNRIFVDGNSAAAL
                     GMVYGGATVCAWYPITPSSSLAEAFQKYCAKLRVDPETGKHRYAIVQAEDEIASIGIV
                     TGAGWNGARAFTATSGPGISLMNEFIGLAYFAEIPVTLIDVQRGGPSTGMPTRTQQSD
                     VAFCAHASHGDTNHVLLFPEDPHECFEFAAKALDLADRLQTPVFVLSDLDIGMNHRLC
                     APFEWDDARRYDRGKVLTAEELEACKPFGRYRDVDGDGIAYRTYPGTHPARGSFFTRG
                     TSKNADAKYSEKGEDYVENMQRLQRKLETAKALLPQPVSHRAPHPAKHGAIHFGSTSS
                     SMAEAIAKLDAKGMHIDALRVRGYPFADSVREFVDRHEQVFVVEQNRDGQLRSLLIEQ
                     FELDPARLVAVLHYDGTPITARFIARAIGERMGGAEVTQLRSDKVA"
     gene            4131..5186
                     /locus_tag="VFK00_00835"
     CDS             4131..5186
                     /locus_tag="VFK00_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET7591532.1"
                     /translation="MTYLPKPKLHHPSLTRNRVGFTRRDYEGAISTLCAGCGHDSISA
                     AIIQACWELDIQPHRVAKISGIGCSSKTPTYFLSASHGFNAVHGRMPSVLTGANLANR
                     ELMYLGISGDGDSASIGIGQFVHAIRRGVDMVYIVENNGVYGLTKGQFSATADQGSKS
                     KKGAINTDAPVDMVGMALQLGASFVARSFSGDKTQLVPILKAAMSHKGASFVDVISPC
                     VAFNNHPGSTKSYDYVREHNHALCRMDFISPREEITVEYPAGEVREVRQHDGSVLRLR
                     KLPESHDTGDRVAAMNYLHACQARGEIVTGLLYVDPEAPDLHAHLDTAPVAFNALGER
                     ELCPGNATLERINAGLR"
     gene            5202..5564
                     /locus_tag="VFK00_00840"
     CDS             5202..5564
                     /locus_tag="VFK00_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591533.1"
                     /translation="MRPPHPFASIARELAIDRNKRRLNRHLDRWQSEIPGWAGRTMGW
                     LRRPSSRWVRIPIGLLLIACGLVGIVLPILGFWMVIPGLLLLAIDVPFLRLPVRVAIV
                     RTRHAIRRFRQRRRARRR"
     gene            5660..6721
                     /gene="phaE"
                     /locus_tag="VFK00_00845"
     CDS             5660..6721
                     /gene="phaE"
                     /locus_tag="VFK00_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaE"
                     /protein_id="HET7591534.1"
                     /translation="MANKADSTDPLGDWQSRSRQYWDAWADATRKAFGAATAGADVPQ
                     GPWHEGLDQWARMFDARKTRESQNEVVERMLAGARGYFSMLESLTARGGAAGGADPHA
                     WADALRESFNFPGAAPELFDNPLARAMRELAGHGAKGFEQMMQGLEPAMAEVRALLDM
                     PAFGYAREHQEHYQRMAKAWLDYQRESNRYNTLIARASRRAFEVFEDKLAERGESGRQ
                     VDSVRGLYDLWVDAAEDAYAEIALSDEFREVYGALVDAQMRVRQNLQREVERVATDLG
                     MPTRSEIDSMGKRLHELRREARNRAETLPALAAEVAALRAEVERLKSGGKPAAARAPR
                     ATAKATPRKSRKSTRKEKA"
     gene            6718..7788
                     /gene="phaC"
                     /locus_tag="VFK00_00850"
     CDS             6718..7788
                     /gene="phaC"
                     /locus_tag="VFK00_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HET7591535.1"
                     /translation="MMDPIRIDPKRALDEALHAQQKLAASMQTLREVEDVDYGATGKQ
                     AIYREDKVVVYRYEGARKPTAKVPILICYALVNRPYMVDLQNDRSLVKNLLELGEDVY
                     IIDWGYPDGADRWLDLDDYINGYIRRCVDAVAKHAGVKKINLLGICQGGAFSLCFASL
                     HQEKLQNLITMVTPVDFHTSDNMLSHWTRDMDVDLFVDTLGNVPADLMNWCYLTLKPV
                     RLNQQKYVAMLDILDDKVELENFLRMEKWIFDSPDQAGEAFREFIKEFYQGNKLVKGG
                     LKIGGRDVSLRNITLPVLNIFALHDHLVPPSASRPLGEHIGTKDYTQLEFKGGHIGIY
                     VSGRAQREVPSAIHEWLAKRTK"
     gene            7906..8103
                     /locus_tag="VFK00_00855"
     CDS             7906..8103
                     /locus_tag="VFK00_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7591536.1"
                     /translation="MNTRRPLRRSRHDRMIAGVVGGLAGYFGIDSSMLRIIYVLVSIF
                     SAAFPGILVYIILWLVVPQEA"
     gene            8176..9216
                     /gene="mtnA"
                     /locus_tag="VFK00_00860"
     CDS             8176..9216
                     /gene="mtnA"
                     /locus_tag="VFK00_00860"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET7591537.1"
                     /translation="MNHLGDEGGDHDTIRAVQWRDDHLRLLDQRKLPLAEEWVHCRAA
                     EDVAQAIRGLVVRGAPAIGIAAAWAVVLAAQHGDEIGPALETLRAARPTAVNLMAALD
                     RMRARIDGGADAAALAREAQAIQDEDLAANRAMGELGSALIQPGSGVLTHCNTGSLAT
                     AGFGTALGVIRAGRASGRIERVYAGETRPWLQGARLTMWELQRDGIPATLIADAAAAH
                     LMRSGEVQWVIVGADRIAANGDTANKIGTYQLAIAAKHHGVKFMVVAPSTTVDMAAAD
                     GTGISIELRDAAELLGYGGQRTVVEGAGAWNPVFDITPHELIDVLVTERGAIPRPDQA
                     SLAASFGPPATR"
     gene            9217..9663
                     /locus_tag="VFK00_00865"
     CDS             9217..9663
                     /locus_tag="VFK00_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7591538.1"
                     /translation="MWHVSKQLGWFGVGGFIGFVVDAGIVQLLVSKLHTDPYVGRLFS
                     FTCAATATWLFNRHFTFHERGEYTLFGEWSRYMVAMAAGFALNYTTYALIVYFSHFVQ
                     DWPSIGVAAGSLPGSAVNFLGARQWVFSGDKKGGAARAREEPKNRP"
     gene            9795..12386
                     /gene="gyrA"
                     /locus_tag="VFK00_00870"
     CDS             9795..12386
                     /gene="gyrA"
                     /locus_tag="VFK00_00870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7591539.1"
                     /translation="MAEPASEVIRVNIEDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMNELGNAWNKSYKKSARVVGDVIGKYHPHGDASVYDAIVRMAQPFSLRYML
                     VDGQGNFGSVDGDSPAAMRYTEVRMSRLTHELLADIDKETVDFGPNYDESEHEPLVLP
                     TRVPNLLVNGSAGIAVGMATNIPPHNMREVIDATVALIDSPDLSIDELMQHVPGPDFP
                     TAGIINGAAGILQAYRTGRGRVLVRAKTEIETNDHGHEAIIATEIPYQVNKARLIEKI
                     AELVKEKKVEGISGLRDESDKDGMRIVIEVRKDAMADVVLNNLFQQTQLQVTFGINMV
                     ALVDGQPRLLNLKEILEAFIRHRREVVTRRTVFDLRKARSRAHVLEGLTVALANIDEM
                     IELIKTSESPQVARERMLARKWEPGVVRALLQAAGTTASRPEDLEAGAGLAEDGYQLS
                     EIQAREILEMRLHRLTGLEQDKLSDEYRQLLETIRGLIAILEDPDKLLAVIRTELVEM
                     KDLYGDTRRTEILHTQEDLETLDLIAQEDVVVTLSHTGYCKRQSLDTYRAQKRGGRGR
                     SAASVKEEDFVERLWIVNTHDTLLTFTSAGRVYWLNVYRIPEASPGARGRPIVNLLPL
                     QDDETVQAVLPVREYDEDHFVFFATRQGTVKKTPLKEFEFQLQKGKLAISLDEGDGLV
                     EAELTDGNCDVLLFASNGKAARFAGGEVRPMGRTARGVRGMRLAEGAHVVSMIVADGN
                     TEAGDCDVLTATERGYGKRTPLADFPRKGRGSQGVIAIQCSGRNGALVAAVATDASHE
                     LMLISNHGTLVRTRVAEVSQVGRNTQGVTLIRLPEDEKLVGVVKIENGDEEGTEIDGA
                     AENAPVA"
     gene            complement(12608..13249)
                     /locus_tag="VFK00_00875"
     CDS             complement(12608..13249)
                     /locus_tag="VFK00_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7591540.1"
                     /translation="MPIEAVIFDCDGTLVDSERLANAALAECVAPFGLRMTAEQAMQA
                     YVGGKMADCVADLECRLGQKLPDTFIPEVRRRTAEAFRMHLKPVEGALDVLQKLQLPF
                     CVASSGPMEKIELSLALTGLARFFTGDRIFSAYDIGSWKPDPGLFLHAAKAMGVAPER
                     CAVVEDSLRGVEAGTNAGMRTFWFQPQGPLPPSVEALHRLADLPELLAQTAAV"
     gene            complement(13309..>13603)
                     /locus_tag="VFK00_00880"
     CDS             complement(13309..>13603)
                     /locus_tag="VFK00_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET7591541.1"
                     /translation="DFRKRGIDVACMAVNDAFVMKAWADHRHVPEGMLMLADGNGEFV
                     KALGLEMDSSKYGMGLRAKRFALYAEDGVVKVLHIESPGEFRVSSAEAMLEAL"
BASE COUNT         2453 a         4469 c         4494 g         2187 t
ORIGIN      
        1 cgaaacgggt agactcgcct cgacgggcga cgccggaagg tccgacgcca ccgcggacgg
       61 caggaggcgt tgcgtgcaac ccaccgatat ccgcaatccg cagtatttcc acaaggtggt
      121 ggattgccag tgggcgtgcc cggcgcatac ccaggtgccg cgctatatcc ggctgattgc
      181 ggcggggcgc tacgacgacg cctacctgct gaactgggat tccaacgtct ttcccggcat
      241 cctcggacgt acctgcgacc ggccttgcga acccgcctgc cggcgcggac gcgtggagga
      301 aaacaacggc cccccccaag acaaaacgaa gcccgagccg gtcgcgatct gccgcctgaa
      361 gcgcgtcgcc gcggacaacc gcagcgacgg catcgaaacg cggatgcgcg aacgattgcc
      421 cgagccggtg ccgggcgccg atcggtttcg cgttgcctgt atcggcgcgg gccccgcatc
      481 gttggccctg gcgcgcgatc ttgcgccgct cggctatcgc gtcacgatct tcgaggccga
      541 cgccaagggc ggcgggttca tgcgcaccca agtgccgcgc ttccgtttgc cggaagtggt
      601 gatcgacgag gaggtcggtt acatcctcga cctcggcgtg aatttcgtcg gcaaccggcg
      661 catcgattcg ctgcgcaagg tgctcgacga aggcttcgat gcgattttcg tcggttgcgg
      721 tgcgccgcgc gggcgcgacc tgcacattcc gggcagggac gagaacaccg tcaagatcca
      781 tgtcgggatc gagtggctgg cgtcggtgta tttcggacac gtgacgaagg tcgggcggcg
      841 ggtgctggtg ctgggtggcg gcaacaccgc gatggattgc tgccgcagcg cgcgccgcct
      901 cggtggcgac gacgtgaaag tcatcgtgcg ttcgggctac gacgagatga aagccagtcc
      961 ctgggaaaaa gaggacgccg aacacgaggg cgtcgagatc ctgaactggc tgaatccgaa
     1021 gtcattcgtg cacgagaacg gcaaactcac cggcatgtgg ttcacgccga tgcgcgcgga
     1081 gcgcgacgcc aacggcaggc gcaagctgct gccgacaggc gagccggatc gcctgttccc
     1141 gtgcgatgaa gtgctgatcg cggtggggca ggaaaacgcc tttccatgga tcgagcgcga
     1201 cctctgcctg gaattcgacg aatggggcac gccgaagctc gacaaggtca cgatgcaatc
     1261 cacgctgccc agggtcttct tcggcggcga cgcggcgttc gggcccaaga acatcatctg
     1321 ggcggtcgag cacggacacc aggcggcgat ctcgatccac aacttcctgc aggggacgga
     1381 cttgcgtgaa cgcccgtcgc cgttgaccaa tctcgtctcg cagaagatgg gcatccatga
     1441 atggagctac gacaacgaca tttcgccgga tgtgcgttac gtggtcccgt gggcgccgaa
     1501 ggaaaaaacc ctcacgaata tccaccagga ggtcgagctc ggcttcgacg cgcaaaccgc
     1561 ctggaaggaa gcgcagcgct gcctcaactg cgacgtgcag acggtgttct cgaaggatct
     1621 ctgcatcgag tgcgacgcct gcgtcgacgt gtgcccgatg gactgcatca ccttcaccgc
     1681 cgacggcgag gaagccgaac tgcgcacgcg cctgaccgca ccggcaaaca ataccgggca
     1741 ggatttgtat gtttccgggc ccgtcaagac cggacggata atggcgaagg acgaggacgt
     1801 atgcctgcac tgcggcctgt gtgccgagcg ctgcccgacc ggcgcgtggg acatgcggca
     1861 gttcctgttc gccgaaagct acgcggcgcg cggcgaggat tattcgcccc ggccggcgaa
     1921 aaaggtggcg gcgtgaagtc ggactccgta gcagtcccga ttccgcttga acccctctcc
     1981 ccccggaaga ggggcagggg tgagggttcg gtactcctgt gccgattcgg tatccccgaa
     2041 cccccacccg ccccttcggg gcaccctctc ccggagggag ggcggtacgc atctggaaca
     2101 tttggcgcga aatgaccgcc atcaccgcca ccaacgactt cgtcgtcaag ttcgcgaacg
     2161 tcaacgggtc gggctccgct tccgccaacg aactgttcgc gaaatgcatc ctgcggatgg
     2221 gcgtgccggt cagtccgcgc aacatcttcc cctcgaacat ccaggggctg ccgacctggt
     2281 acgaagtgcg cgtcagtgaa gcgggctggc tcggcaggcg cggcggctac gacctgctgg
     2341 tcgcgatgaa cccgcagacc tgggacgccg atgtcgcggg gctctcgccg ggcggctatc
     2401 ttttccatga caacagcaag ccgctcgccc cggcgaagtt ccgcgacgac gtcaccgtga
     2461 tcggggtgcc gctgaccgac atcgccaacc ggacctggga cgaccctcgc cagcggcaac
     2521 tattcaagaa catcatgtac ctcggcgcgc tgtcggtgtt gctcggcatt gaacgcgacg
     2581 taatcgttca actggtcggc gagcagtaca aaggcaagca ggcgctgctg gagcccaaca
     2641 tccgcgcgct cgatctcggg cgcgattacg cccagcacaa cctgccgccg gtgggcctga
     2701 agatcgaacg ccgggacaac gtgggcaatc gcattttcgt ggacggcaac agcgccgccg
     2761 cgctcggcat ggtgtacggc ggcgccaccg tctgcgcgtg gtacccgatc acgccgtcct
     2821 cgtcactggc cgaggcgttc cagaaatatt gtgcgaagct gcgcgtcgac ccggaaaccg
     2881 gcaagcaccg ctacgcgatc gtgcaagccg aggacgagat cgcctcgatc ggaatcgtca
     2941 ccggcgccgg ctggaacggc gcccgtgcgt ttaccgcgac ctcggggccg ggcatttcac
     3001 tgatgaacga attcatcggg ctcgcgtatt tcgcggagat cccggtcacc ctgattgacg
     3061 tgcagcgcgg cggtccgtcc accggcatgc ccacgcgcac ccagcagtcg gacgtggcgt
     3121 tctgcgcgca tgcctcgcac ggcgacacca atcatgtgct gctgtttccg gaggacccgc
     3181 acgagtgctt cgaattcgcg gcgaaggcgc tggacctcgc cgatcggttg cagacgccgg
     3241 tgttcgtgtt gagcgacctc gacatcggca tgaaccatcg cctgtgcgca ccgttcgaat
     3301 gggacgacgc gcgccgctat gaccgcggca aggtgctgac cgcggaggaa ctggaagcct
     3361 gcaagccgtt cggccgctac cgggacgtcg atggcgacgg catcgcctat cgcacctatc
     3421 ccggcaccca tcctgcgcgc ggcagctttt tcacccgcgg caccagcaag aacgccgacg
     3481 ccaagtattc cgagaagggg gaggattacg tcgagaacat gcagcgcctg caacgcaagc
     3541 tggagacggc gaaggcgctg cttccgcagc cggtgtccca tcgggcgccg cacccggcaa
     3601 agcatggcgc gatccatttc gggtcgactt cgtcctcgat ggcggaagcc atcgccaaac
     3661 tcgatgccaa ggggatgcac atcgacgcgc tgcgggtgcg tggctatccg ttcgccgatt
     3721 cggtgcgcga attcgttgac cggcacgaac aggtattcgt ggtcgagcag aaccgcgacg
     3781 ggcaactgcg ctcgctgctg atcgaacagt tcgaactcga tcccgcgagg ctcgtcgcgg
     3841 tcctgcacta cgacggcacg cccatcaccg cacgcttcat cgcgagggca atcggagagc
     3901 gcatgggcgg agcggaagtc acgcagttgc ggagcgacaa ggtggcttag ctcttcgttc
     3961 tcgcttcggg agaaggggcg tgcaacgccg ctttttccgt tgcgcgaagc gccggatgag
     4021 ggccaggtgc ttcgcgatcg gcaacatcga ggcggattcc gcgcaattgc ctcccggacc
     4081 ctcaccccaa cccctctccc caagggagag gctcaaggcg ataccccgac atgacgtatc
     4141 tcccaaaacc caaactgcac cacccgtcgc tgaccaggaa ccgcgtcggt ttcacgcggc
     4201 gcgactacga aggcgcgatt tcgaccctgt gcgcgggttg cgggcacgat tcgatttcgg
     4261 cggcgatcat ccaggcgtgc tgggaactcg acatccagcc gcatcgggtc gcgaagatct
     4321 ccgggatcgg ttgcagttcc aagaccccga cctacttcct gtcggcgtcg cacgggttca
     4381 acgcggtgca cgggcggatg ccgtcggtgc tgaccggcgc gaacctcgca aatcgagaat
     4441 tgatgtacct cgggatttcc ggcgacggcg attccgcgtc catcggcatc ggccagttcg
     4501 tgcacgcgat ccgccgtggc gtagacatgg tgtacatcgt cgagaacaat ggcgtgtacg
     4561 ggctgaccaa gggacagttc tccgcgaccg cggaccaggg cagcaagtcc aagaagggcg
     4621 caatcaacac cgacgcgccg gtcgacatgg tcggcatggc gctgcaactg ggcgcgagtt
     4681 tcgtcgcccg cagcttttcc ggcgacaaga cgcaactggt gccgatcctg aaggcggcga
     4741 tgagccacaa gggcgcttcg ttcgtcgacg tgatcagccc gtgcgtcgcg ttcaacaacc
     4801 atccgggcag caccaagagc tacgattacg tgcgcgagca caaccatgcg ctgtgccgga
     4861 tggacttcat ctccccgcgc gaggaaatca ccgtcgagta cccggccggg gaagtgcgcg
     4921 aggtgcgcca gcacgacggc agcgtgctgc gcctgcgcaa gctgccggaa tcgcacgaca
     4981 ccggagatcg cgtcgcggcg atgaattacc tgcatgcttg ccaggcgcgc ggcgaaatcg
     5041 tcaccgggtt gttgtacgtt gatcccgagg cgcccgacct gcacgcgcac ctcgacacgg
     5101 cgccggtcgc gttcaacgcg ctgggcgagc gcgagctttg ccccgggaac gcgacgctcg
     5161 agaggatcaa cgcagggctg cgttgagccg tcacgctgca tgtgcgcccg ccgcacccgt
     5221 tcgccagcat cgcaagggag cttgccatcg acaggaacaa gcgacggctg aatcgacacc
     5281 tggaccgctg gcaaagcgag attccaggct gggccggccg cacgatgggc tggctgcgca
     5341 ggccctcgtc gcgctgggtg cggattccca tcgggctgct gctgatcgcc tgtgggctgg
     5401 tcggcatcgt cctgcccatc ctcgggttct ggatggtgat tcccggcctg ctcctgctcg
     5461 cgatcgacgt cccgttcctg cgcctgcccg tgcgcgtggc gatcgtgcgc acccgtcatg
     5521 cgatacggcg gtttcgccag cgccggcgag cccggcgccg ttgattcggc tggcgcggtc
     5581 gcgcgggctc ccgcgccttt tgttgcggcg caacatgggc gccgatacca tagcgggtca
     5641 gcaatcggag atcagtttca tggccaacaa ggcggattcc accgacccgt tgggcgactg
     5701 gcaaagccgg tcgcggcagt actgggacgc ctgggccgac gccacgcgca aggcgttcgg
     5761 ggcggcgaca gccggggccg acgtgccgca gggcccctgg catgaaggcc tcgaccagtg
     5821 ggcgcggatg ttcgatgccc gcaagacccg tgagtcgcag aacgaggtgg tcgagcgcat
     5881 gctggccggg gcgcgcggct acttctcgat gctggagtcg ctgaccgcca ggggcggcgc
     5941 cgctggcggt gccgatccgc acgcgtgggc ggacgcactg cgcgagtcct tcaatttccc
     6001 gggcgcggct ccggagctgt tcgacaatcc gctggcgcgc gcgatgcgcg agctcgccgg
     6061 gcacggcgcc aagggcttcg agcagatgat gcagggcctt gaacccgcca tggccgaagt
     6121 ccgcgcgctg ctggatatgc cggccttcgg ctacgcgcgc gagcaccagg aacactacca
     6181 gcggatggcc aaggcctggc tggattacca gcgcgaaagc aaccgctaca acaccctgat
     6241 cgcccgcgcc agccggcggg cgttcgaggt gttcgaggac aagctggccg agcggggcga
     6301 atcgggccgc caggtcgact ccgtgcgcgg cttgtacgac ctgtgggtgg atgcggccga
     6361 ggacgcgtac gccgagatag cgctgtccga cgaattccgc gaggtgtacg gcgcgctggt
     6421 cgatgcgcag atgcgggtgc ggcagaacct gcagcgcgag gtcgagcgcg tcgcgaccga
     6481 cctcggcatg cccacccgca gtgaaatcga cagcatgggc aagcgcctgc acgagctgcg
     6541 ccgcgaggcc aggaaccgcg ccgagacgct gcctgctctg gccgccgagg tggcggcgct
     6601 gcgcgccgag gtcgagcgcc tgaagtcggg gggcaagcct gccgcggcga gggcgcctcg
     6661 tgcgaccgcc aaggccaccc cgcgcaaatc ccgcaaatcg acccgcaagg agaaggcatg
     6721 atggatccga tccgcatcga tccgaagcgg gcgctcgacg aggccctgca cgcgcagcag
     6781 aagctggcgg cgagcatgca gaccttgcgc gaagtcgagg atgtcgatta cggcgccacc
     6841 ggcaagcaag cgatctaccg cgaagacaag gtcgtcgtct atcgctacga gggtgccagg
     6901 aagccgaccg ccaaggtgcc gatcctcatc tgctacgcgc tggtgaaccg gccgtacatg
     6961 gtcgacctcc agaacgaccg ctcgctggtc aagaacctgc tcgagctcgg cgaggacgtc
     7021 tacatcatcg actggggtta cccggatggc gccgaccgct ggctagatct cgacgattac
     7081 atcaacggct acatccgccg ttgcgtcgat gcggtcgcga agcatgcggg cgtcaagaag
     7141 atcaacctgc tcggcatctg ccagggcggc gcgttctcgc tgtgcttcgc gtcgctgcac
     7201 caggagaagc tgcagaacct gatcacgatg gtgacgccgg tcgacttcca taccagcgac
     7261 aatatgctgt cgcactggac ccgcgacatg gacgtggacc tgttcgtgga tacgctgggc
     7321 aacgtgccgg cggacctgat gaactggtgc tacctcacgc tgaagccggt gcgcctgaac
     7381 cagcagaagt acgtcgcgat gctggacatc ctcgacgaca aggtggaact cgagaacttc
     7441 ctgcggatgg agaagtggat cttcgactcg ccggaccagg ccggcgaagc gttccgcgaa
     7501 ttcatcaagg agttctacca gggcaacaag ctggtcaagg gcggcctgaa gatcggcgga
     7561 cgggacgtca gcctcaggaa catcacgctt ccggtgctca acatcttcgc gctacatgat
     7621 catcttgtgc cgccttcggc gtcgcggccg ctgggcgagc acatcggaac caaggactac
     7681 acccagctcg agttcaaggg tggtcacatc ggcatctacg tgtccggacg cgcccagcgc
     7741 gaggtgccgt cggcgatcca cgagtggctg gccaagcgca ccaagtagcc gggcagggcg
     7801 caacccgcga agcgctttcc gcctcggccg ccgtcgcgga agcggctgcg cagattccgc
     7861 cctacgcagc tacatcacga ttggcggccg gatagaatcg tggccatgaa cacacgacgt
     7921 cccctgcgcc gttcgcgcca cgaccggatg attgccggcg tggtcggcgg cctcgccggt
     7981 tacttcggga tcgactcgtc gatgctgcgc atcatctacg tgctggtgtc gatcttctcc
     8041 gcggcgtttc ccggaatcct ggtctacatc atcctgtggc tggtcgtgcc gcaggaagca
     8101 tgaccgaccg ctgcacgcgg ccgacggatc tggcgcgtgc taggctctcg ctcgaagcgg
     8161 gcggcgtgat tcgacatgaa ccatctgggc gatgaggggg gcgatcacga caccatccgc
     8221 gccgtgcaat ggcgcgacga tcacttgcgt ctgctcgacc agcgcaagct gccactcgcc
     8281 gaggaatggg tccattgccg ggccgccgaa gacgtagcgc aggcgattcg cgggctggtc
     8341 gtgcgcggcg cgcctgcaat cgggatcgcc gcggcgtggg ctgtggtgct cgcggcgcag
     8401 catggggatg aaatcggccc cgcgctggaa accctgcgcg ccgcgcgtcc gacggccgtc
     8461 aacctgatgg ccgcgctgga ccggatgcgc gcgcggatcg acggcggggc cgatgcggcg
     8521 gcgctcgcgc gcgaggcaca ggcgatccag gacgaggacc tcgccgccaa tcgcgcgatg
     8581 ggcgaattgg gttcggcgct gatccagccc ggtagcggtg tcctcaccca ttgcaatacc
     8641 ggttcgctcg cgaccgcagg tttcggcacg gcgctgggcg tgatccgggc aggccgcgcg
     8701 tccggcagga tcgagcgcgt gtacgcgggt gaaacccggc cgtggctgca gggggcgcgc
     8761 ctgacaatgt gggaactgca gcgcgacggc attccggcca ccctgatcgc cgatgccgcg
     8821 gccgcgcacc tgatgcggtc cggcgaggtg cagtgggtga tcgtcggtgc ggaccggatc
     8881 gccgcgaacg gcgacaccgc caacaagatc ggcacctacc agctcgcaat cgccgcgaag
     8941 caccacggcg tcaagttcat ggtggtggcg ccttcgacga ccgtggacat ggccgccgcg
     9001 gacggcaccg gcataagcat cgaattgcgc gatgccgcgg aactgctcgg ctacggtggc
     9061 cagcgcacgg tcgtggaagg cgccggcgcg tggaatccgg tgttcgacat cactccgcac
     9121 gagttgatcg acgtgctggt gaccgagcgc ggcgcgatcc cgcggccgga ccaggcatcc
     9181 ctcgccgcgt cgttcggccc accggcgaca cgctgaatgt ggcacgtctc caagcaactg
     9241 ggatggttcg gggttggcgg cttcatcggc ttcgtcgtcg atgccggcat cgtgcagttg
     9301 ctggtcagca aattgcacac ggacccgtac gtagggcgct tgttttcgtt cacctgcgcg
     9361 gccaccgcga cctggttgtt caaccggcac ttcaccttcc acgagcgggg cgagtacacg
     9421 ctgttcggcg aatggtcgcg ctacatggtc gccatggcgg ccggttttgc gctcaattac
     9481 acgacctacg ccctgatcgt ctacttctcg catttcgtgc aggactggcc gtcgatcggc
     9541 gtcgcggcgg gctcgctccc cggttccgcg gtgaacttcc tgggggcccg ccagtgggtg
     9601 ttttccggcg acaagaaggg gggcgcggcc cgagcccggg aagagcccaa aaaccgcccg
     9661 taagttcgcg cgcccgttga ggttttttgg cctgtttcgg ggccggttgt ggtagaattc
     9721 caaggttgat ttcgaggcgc gggttgcgtt ccggaccgcg ccttttgcat tcacccgata
     9781 caggaattct gcggatggcg gaaccggcca gtgaagtaat tcgcgtcaat atcgaagacg
     9841 agatgcgcca gagctacctc gattacgcaa tgagcgtgat cgtgggccgc gcgctgccag
     9901 atgtgcgtga cggcttgaaa ccggtgcatc gccgcgtgtt gttcgcgatg aacgagcttg
     9961 gcaacgcctg gaacaagtcc tacaagaaat ccgcgcgcgt ggtcggcgac gtgatcggca
    10021 agtaccatcc gcacggcgat gcgtccgtgt acgacgcgat cgtgcggatg gcccagccgt
    10081 tctcgctgcg ctacatgctg gtcgacggcc aaggcaactt cggctcggtc gacggcgaca
    10141 gcccggcggc catgcggtac accgaagttc gcatgtcgcg cctgacccat gagttgctcg
    10201 ccgacatcga caaggaaacc gtcgacttcg gtcccaacta cgatgaaagc gaacacgaac
    10261 cactggtcct gccgaccagg gtgccgaacc tgctggtcaa cggctccgcc ggcatcgccg
    10321 tcggcatggc gaccaacatc ccgccgcaca acatgcgcga ggtaatcgac gctaccgtcg
    10381 cgttgatcga cagcccggac ctctcgatcg acgagttgat gcagcacgtc ccggggccgg
    10441 acttccccac cgcggggatc atcaacggcg cggccgggat cctccaggcc taccgcaccg
    10501 gccgcgggcg ggtgctggtg cgcgccaaga ccgaaatcga aaccaacgac cacggtcacg
    10561 aagcgatcat cgcgaccgag atcccgtacc aggtcaacaa ggcgcgcctg atcgagaaga
    10621 tcgccgaact ggtcaaggaa aagaaggtcg agggcatcag cggcttgcgc gacgagtccg
    10681 acaaggacgg catgcgcatc gtgatcgagg tgcgcaagga cgcgatggcc gacgtggtgc
    10741 tgaacaacct gttccagcag acccagctgc aggtgacttt cggcatcaac atggtggcgc
    10801 tggtggatgg ccagccacgc ctgctgaacc tcaaggagat cctcgaggcc ttcatccgcc
    10861 accgccgcga ggtggtcacc cgccgcaccg tcttcgacct gcgcaaggcg cgctcgcgtg
    10921 cgcatgtact ggaaggcctc acggtcgcgc tcgccaacat cgacgagatg atcgagctga
    10981 tcaagacgtc cgagtccccg caagtcgcgc gcgagcggat gctggcgcgc aagtgggagc
    11041 cgggcgtggt gcgcgccttg ctgcaggccg ccggcaccac tgcctcgcgc ccggaggacc
    11101 tcgaagccgg cgccggcctc gcggaggacg gctaccagct ctcggaaatc caggccaggg
    11161 aaatcctgga gatgcgcctg caccgcttga ccggcctcga acaggacaag ctcagcgacg
    11221 aataccggca gttgctggag acgatccgcg gactgatcgc gatcctcgag gacccggaca
    11281 agctgctcgc ggtgatccgc accgaactgg tggagatgaa ggacctgtac ggcgatacgc
    11341 gtcgcaccga gatcctgcac acccaggaag acctggaaac actggacctc attgcgcagg
    11401 aagacgtcgt cgtcaccctc tcgcacacgg gctattgcaa gcgccagtcg ctggatacct
    11461 accgcgcgca gaagcgcggc ggcaggggcc gttccgcggc cagcgtgaag gaagaggatt
    11521 tcgtcgagcg cctgtggatc gtcaacaccc acgacacgct cctgaccttc accagcgccg
    11581 ggcgcgttta ctggctcaac gtgtaccgca ttccggaagc gagccccggc gcgcgcggcc
    11641 gccccatcgt caacctgctt cctctgcagg acgacgaaac cgtgcaggcg gtgctcccgg
    11701 tgcgcgagta cgacgaggat catttcgtgt tcttcgcgac ccgccagggc acggtgaaga
    11761 agacgccgct gaaggagttc gagttccagt tgcagaaggg caagctcgcg atcagcctcg
    11821 acgaaggcga cggactggtc gaggccgaac tcaccgacgg caactgcgat gtcctgttgt
    11881 tcgcgtcgaa cggcaaggcc gcccgcttcg cggggggcga ggtccgtccg atgggccgca
    11941 ccgcccgtgg cgtgcgcggc atgcgcctgg ccgaaggcgc ccacgtcgtc tcgatgatcg
    12001 tcgccgacgg gaacaccgaa gccggggact gcgacgtgct gaccgccacc gaacgcggct
    12061 atggcaagcg caccccgctc gccgacttcc cgcgcaaggg ccgcggctcc cagggcgtga
    12121 tcgcgatcca gtgctcgggc cgcaacggcg cgctggttgc ggcggtcgcg accgacgcca
    12181 gccacgagct gatgctgatc tccaatcacg gcacgctggt acgcacccgc gtcgccgaag
    12241 tttcgcaggt cggccgcaac acccagggcg tcaccctgat ccgcctgccg gaagacgaga
    12301 agctcgtcgg cgtggtcaag atcgagaacg gcgacgagga aggcacggag atcgacggcg
    12361 cagccgagaa cgcgccggtc gcgtaatccc gggccgtagg ttgggttgag ccgaggttcc
    12421 acctcggcga agcccaacaa tcccgcgaca cccgtctgcc cggattccgt ggcccggata
    12481 agccgcaaag cggcgcaatc cggggcatgc cgtgcgtgca ttcccgtatt ccgcttcgtt
    12541 tcatccgggc tacttgccgg cctgcgcgca tctcgttggg cttcgcgaag aagccgctca
    12601 acccaaccta cacggccgcc gtttgcgcga gcagctccgg caggtccgcg agtcggtgca
    12661 gcgcctccac cgatggcgga agaggtccct gcggctggaa ccagaaggtg cgcatgcctg
    12721 cgttcgtgcc cgcctcgact ccgcgaaggc tgtcttccac gacggcacag cgttccgggg
    12781 caacgcccat cgccttggct gcgtgcagga acaatccggg atccggtttc cacgagccga
    12841 tgtcgtacgc gctgaagatc ctgtcgcccg tgaagaagcg cgcgaggccc gtcagcgcca
    12901 ggctcaattc gatcttttcc atcgggccgc tcgaagccac gcaaaacggc agctgcagct
    12961 tttgcagcac gtccagcgcg ccctcgacag gcttgagatg catccggaac gcttccgcgg
    13021 tccgcctgcg gacctccggg atgaaagtat ccggcagctt ctggcccaac cgacactcga
    13081 gatcggcgac gcagtcggcc atcttgccgc cgacgtaggc ctgcatcgcc tgctccgcgg
    13141 tcattcggag accgaacggc gccacgcact ccgccaacgc cgcgttggcc agccgctcgc
    13201 tgtccacgag cgtgccatcg cagtcgaata tcaccgcttc gattggcatc gagtcgtcca
    13261 gcaaggacac ggcgggaaac cgccgccggt cacgcgcatg gtgcactcct acaacgcctc
    13321 cagcatggcc tcggcgctcg acacccggaa ctcgcccggc gattcgatgt gcagcacctt
    13381 gaccacgccg tcctcggcgt acagcgcaaa gcgtttcgcg cgcaggccca tgccgtactt
    13441 gctggaatcc atttccagcc ccagcgcctt gacgaactcg ccgttgccgt cggccagcat
    13501 cagcatgcct tcggggacgt ggcggtggtc ggcccaggcc ttcatcacga aggcgtcgtt
    13561 gacggccatg caggccacgt cgatgccgcg cttcctgaaa tcc
//
LOCUS       DASSNH010000026        13834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1884952, whole genome shotgun sequence.
ACCESSION   DASSNH010000026 DASSNH010000000
VERSION     DASSNH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884952"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..959
                     /locus_tag="VFK00_00885"
     CDS             375..959
                     /locus_tag="VFK00_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591542.1"
                     /translation="MARSRGGAGRQLEDSVVVEGEGGWIEEITTTYVVVRIRDLRRLI
                     LPLSCFIEKPFQNWTRTTANLLGTAYIYADYTVPVQAVRDELERIPEEDPLYDGFAWS
                     LDLTNLTENVIEMRAMVSARNAGDTVNLRRHVRERLSRSCRRTIPNACRAPASKSRAA
                     ARPARATPKACCRSRPGEFRLVLTGDRSPPGNVN"
     gene            1151..2095
                     /locus_tag="VFK00_00890"
     CDS             1151..2095
                     /locus_tag="VFK00_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HET7591543.1"
                     /translation="MKIRVLGTNPWAGLLVLVAGIGFASAGWAAPDAQPQMPRCSSRI
                     GTLAVQEPQTGNQWWTQMNLESPAALIKVYVSQSGCFTLVDRGKGLQAARAERELAGE
                     GEMRQGSNIGMGQMKAADYVLVPDIANSNSNASGNRIGGLLGGFLPHGIGAVVGGISI
                     SKKTADVVLTLTDIRSTEQVALEQGHANKTDLGWAGGGGGGFWGGFAAGGASGYANTE
                     IGQVIAMAYLDAYTKMVADLQHNAPNAQADNVQQAVRLTEESKLYADANLHSSVVRTL
                     KPGMMLYPTGDKVGIWWKVTDELGNIGWVLSSKVELAH"
     gene            complement(2177..2860)
                     /locus_tag="VFK00_00895"
     CDS             complement(2177..2860)
                     /locus_tag="VFK00_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET7591544.1"
                     /translation="MTTSPARVRIDFVSDVACPWCAIGLASLQRALAALDETVQADIY
                     LQPFELDPQMPLEGEDADQRLMQKYGIGEAQLEENRRVIRDRGAPLGVAINTGRGSRA
                     WNTFDAHRLLHWAGIQDRERAVELKQALFRAYFTDQENVADRQVLVRIATDVGLDAAQ
                     ARGVLDSDAYAEEVRTLEQRFQQAGIHSVPATIINGKWVIPGGQPPETFEGALRDIAS
                     KEQPAAGTA"
     gene            3062..4273
                     /locus_tag="VFK00_00900"
     CDS             3062..4273
                     /locus_tag="VFK00_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET7591545.1"
                     /translation="MDEDNAVAVDTSSLAAKSPPHQAQRLAALRSYDILDTPVEGAFE
                     DITCIASLVCRAPIAAVNLVDENRQWFKSEVGLGVREMPLDASFCAHALLQNEFLEVP
                     DIELDPRFRDNPLVVDGPRLRFYAGALIRTAEGLALGTVCVMDSRPRRLDDEQRRVLR
                     ALARQAMTQLELRRTMIVAARAHRYRGRLMAIAGHDLKQPLTTMTMVLDMLPAATPAD
                     RRRIEVAKEAAARLREELDGLSRASRLEEETDAIELEPLQVDELIAEVADNWLRAAKD
                     KGLGLQVRVIDAEVRTHAGMLRTMLDSLVGNAIKYTSQGSVTIACREEGGDLWIDVAD
                     TGKGIPEGRRDEMFTAFRQLDPDTDGLGLGLSIVKSTADMLGYRLSLECGEGRGATFS
                     VCLPRQAPVHR"
     gene            4340..5215
                     /locus_tag="VFK00_00905"
     CDS             4340..5215
                     /locus_tag="VFK00_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET7591546.1"
                     /translation="MADHPRKDPSNENKPGGGEEPDGSFRLTEREKDEVEENLPPRVQ
                     VLHEAIRVEGKTELGRSVAALSWSSLAAGLSMGFSMLIPALLAVHLVGVAGGFLIVKL
                     GYSIGFLIVVSARQQLFTENTLTAVLPFMTRPHWPEFGRLLRLWGIVLVGNLVGVALF
                     ACGMVYMHVLDAATHAALVSQAEEVMRNTVLQMFTKGIIAGWLLGTMVWLIPALPHAK
                     VIVIVVMTWLIGLGNFTHVIAGSTEVLYLVWRGDLPAADYIVRFMLPTLAGNIVGGSF
                     IFGLISHAQVRSDDS"
     gene            complement(5212..6759)
                     /locus_tag="VFK00_00910"
     CDS             complement(5212..6759)
                     /locus_tag="VFK00_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7591547.1"
                     /translation="MEAEDDSQTPESAPVIAAVELGRWFRRDVEEPLEHIVGGRKRLK
                     VIALLACVLALDAADKATIGAVAAQLEHDLHIGNVEVGLLVTVSTAIGAFATLPFGML
                     VDRMHRGRLLVIGIGLWSLAMIASGAAISYPMMLVSRLGLGAVVAMASPAIASLVGDF
                     FHPAERARIYSYILAGELIGVAFGFLISGNVAAIASWRVPFFILAGLGLVLAVVIARK
                     LPEPKRGGQGRMEDETQDGDAEETHPVHEIVDERGIDPRRERILKEDPEKMSLWRAVR
                     YVLSIPTYRVLIIASALGYFYFTGLRTFAIVFMREHFALQQAMASTVTVAVGVGAIAG
                     VLVAGWAADRMIDHGRLRGRIIAGAAAYFVATLGLAGGLLAPSLIFAAPFFFVAAGGL
                     GGANPAVDSARLDIMHSRLWGRAEGVRSTLLYAFQAAAPPLFGWVSGVFGGGSTSAGR
                     SPAGAGSGHGLDMAFLLMLVALAAAGFVMLRAMQTYPRDVATAIASEHHAPGKDRKRR
                     ARPPAAR"
     gene            6913..8145
                     /locus_tag="VFK00_00915"
     CDS             6913..8145
                     /locus_tag="VFK00_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HET7591548.1"
                     /translation="MVTAARAARKSATPAIWLLAFMMAAGPFGDTEYTPAMPAMAKAL
                     HADYGMIQFTMAAYLAASAVSQLVFGPASDRWGRRPVMLFGAVVLSAGALLCMLSFSI
                     WPLIGGRFIQGVGACAGGVIADAVVRDHFSAKRRQRVYAKINAAFAVAPAVGPVAGTY
                     IAHAFGWHANFALLLAVSLALTYSVWRWLPETLREPDPRALERKRLWSNAREVLGTRG
                     FLFYVAMGGLCVGVVYAALIGAPDLVINVLHQGSGAIVVVAIAILVAFVIGAGACAWL
                     TPRLPHLAIIGGGLALVLAGSILLMLVALFADGKGNLATYLVPIGISFIGVGLTVPVS
                     TAKAMAPFGDNAGAASSVLGFLRMGIAALGTIAMSALHMGSVYDIPIVFLALSVTAVV
                     LFCTYLGLRGKPPEAATH"
     gene            complement(8348..9055)
                     /locus_tag="VFK00_00920"
     CDS             complement(8348..9055)
                     /locus_tag="VFK00_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HET7591549.1"
                     /translation="MSSKPPTRFAGRALPADRIRGGGNPPCRERFPATVLLVACLLFL
                     GGCVHSTKAQFTGHYGLGTTASVPTTDSGAANAGGKILQVSQIAVPDWLSGNAMFYRL
                     DYRNDGRLAAYGQSDWISPPASLLEPILRRALAGGGWRAVIGPRNPATADASLQLRLD
                     DFSQAFAEPGQSKGVLDATATLIDNHDDNVIAQKRFHIEVPAATPDAQGGAKALAEAS
                     RKFAAQLRDWAQAAGGR"
     gene            complement(9048..9983)
                     /locus_tag="VFK00_00925"
     CDS             complement(9048..9983)
                     /locus_tag="VFK00_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7591550.1"
                     /translation="MENRAHAIIAVCFLVVFAIVAALIFLWLTSGPSEPLAYRIVTRQ
                     SVAGLAPQSRVEFKGIEVGHVQRISFDPEDRSRVIVDFNVQRDTYITHATYAVLAMHG
                     ITGGQTLELKLGKGSRELLATSDKHPAEIPLRKGLLAQLEDSAKQSMEDLQAVLDNAR
                     KVLNDENREHIGASIRQIDAATAKLVQIETELMPAMKRMPALVESAQKSLDESHALLA
                     NANKIAVEARGPVKQAGQAADTIQHLGRKLDRQTAPDVEALSQSLLRTSRQLEELIRE
                     LKAKPQSLIFGPAAPPPGPGEPGFQGNDKHGDGHE"
     gene            complement(9987..10811)
                     /locus_tag="VFK00_00930"
     CDS             complement(9987..10811)
                     /locus_tag="VFK00_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7591551.1"
                     /translation="MNAVAADSAAAETVIEIRGLCKRYGRQQVHKGLDLEVRRGEFLS
                     VIGTSGSGKSTLLQQVIGLVKPDAGSIRVLGSPVGELDGEEARLLRRHWGVLFQQGAL
                     FSAFNVFDNVAFPMRELRKEGWSVDENMLRDSVALKLYMVGLKPEDAWKRPAQLSGGM
                     LKRAALARALMLEAELLFLDEPTTGLDPASSAEFDALLGALHKELNLTVMMVTHDLYS
                     MATLSDRIAVLEEGRLITVGTLREVSRFDHPFIRDFFRSRRNDDPLRDLANHENGA"
     gene            complement(10808..11923)
                     /locus_tag="VFK00_00935"
     CDS             complement(10808..11923)
                     /locus_tag="VFK00_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7591552.1"
                     /translation="MAKPASGPTWTREGEARITLSGSWNLLVDKQRRRRLLRQFRRLG
                     APGDYCWDLQHVESLDSTGALVLWRAWQGRLPAQLACSDEQRHLFQRLEDMPALGRPA
                     RTGSLYWLDRLGAGIIAVLATAAGIFLLVGQLMLDIAYCLRHPRVIPWKEISANVYQI
                     GATAMVLLGSIGFLIGAVMTVQLGLALQQFSASDMVIGMMAIAVPRELGAVVTGLLMA
                     GRTGSAMTASIGAMHLTDEYNALQALGSAPSLRLALPRVVGAAVAMPLLVVWTDITSM
                     LGSALVAQAQLGINFRLFLAEAMQQVQIVNFWIGLGKGVLFGLVIALVGSYFGMNSSP
                     DTESMSRNTTSSVVTSLTMILLLDASLGAALTNVGLL"
     gene            12111..13703
                     /locus_tag="VFK00_00940"
     CDS             12111..13703
                     /locus_tag="VFK00_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET7591553.1"
                     /translation="MSSIALRASRLLLGAVALFLAVQAWAAGPAPTVRVDGGRLSGIH
                     DASTGLDEFRGIPFAAPPVGALRWKPPQPLAGWTGVRRADHFAPRCMQRPLFGDMVFR
                     SDGISEDCLYLNVWTPAHRSGGKLPVLVYFYGGGFLAGDGSEYRYDGASLARRGIVTV
                     TVNYRLDVFGFLALPALAAESPHHATGNYGLLDQVAALRWVRRNIAAFGGDPAKVTIG
                     GESAGSMSVSALMASPLSKGLMQRAIGESGAVLGNLGPKPLAVAEREGTDFVRHVGAG
                     SLAQLRAMPAAKLIAACGDPGMPEFGPTVDGWFLPRSPELIYEAGEQAHIPLLVGSNS
                     GEGSYTALLKDAPPTPANYRAAVGREFGTHADEVLKLYPGDSEPEVKASGDALAGDRF
                     IAYSTWRWMHLQRTTGHAPVYYYWFDKARPARRDGSAGPDEAAVHSGEIEYALGNLAT
                     NHVYAWTAADREASATMEGYFANFIKTGNPNGDDLPHWPAATRKDGGLLRQVIGVHTR
                     TIVDRKAPRYEFLQHIEAGAPL"
BASE COUNT         2223 a         4616 c         4740 g         2255 t
ORIGIN      
        1 caaagggcga gcaggtgccc gtgtgacatg acgtagccgc ccatcacgca gacaatcacg
       61 accagcgttc cgaccaaaac caacatgtac gactccgggg cccgtcagcg tgacgtcctg
      121 cgcacccgcg ttatcggctc cgcgccggaa atgtttagcg aactcctgca cataaatccg
      181 catccggcgc gagcgctccg cacgatgccg ccttggccgg cgccgccccg cgaacctgaa
      241 ccggcgcgcg gccgccgtcc cggcagcgcg catccgtcgc caaatcgcga gcgggatcga
      301 ctgcccagga gtgtttggca agggaaatgc ggtggcatgg ataaccaact ggacttcatc
      361 ccgttcgggc cgcattggct cgttcccgcg gtggtgccgg ccggcagctc gaggactcgg
      421 tggtcgtgga aggcgagggg ggctggatcg aggaaatcac cacgacctac gtggtggtgc
      481 ggatccggga tctgcgcagg ctgatccttc cactgtcctg tttcatcgag aagccgttcc
      541 agaactggac gcggaccacc gcgaacctgc tcggcaccgc ctacatctac gccgactaca
      601 cggtgccggt gcaggcggta cgcgacgaac tcgagcggat ccccgaggaa gacccgttgt
      661 acgacggctt cgcctggtcg ctggatctca ccaatctcac tgaaaacgtg atcgagatgc
      721 gcgcgatggt gagcgcacgc aatgccggcg acacggtcaa cctgcgccgc cacgtgcgcg
      781 aaaggctgtc gcgttcctgc agaaggacta tcccgaatgc ctgccgcgca cccgcgtcga
      841 aatcgcgcgc agcggcgcgg cccgcccggg cgacaccgaa ggcttgctgc cgcagccgcc
      901 cgggtgaatt tcgcctggtg ttgacgggcg accggtctcc acccggcaac gtgaattgac
      961 acccgccggg cgccggggta tacggacagc tcccgggcgc cgcgggtcgc gaggtaccgc
     1021 ccggggtgat gtgatcggtg cacctgctgg gcgattcctg ggggacagcg cgctggttca
     1081 ctgccggtgc cgcgtgtccg gtttgttcgg ccatcaccat gccttcctgc caaggaagtg
     1141 ggggatccaa atgaagattc gtgtgcttgg taccaatccg tgggccggct tgctggtgct
     1201 cgttgcgggc atcggcttcg catcggcagg ctgggccgct cccgatgcgc agccgcagat
     1261 gccgcgatgc agcagcagga tcgggacgct cgcggtgcag gagccgcaaa ccggcaacca
     1321 atggtggacc cagatgaacc tcgaatcgcc ggccgcactg atcaaggtgt acgtctcgca
     1381 gtccggatgc ttcacgctgg tcgatcgcgg caaggggctg caggcggcac gcgccgaacg
     1441 cgagctcgcg ggcgagggcg agatgcggca gggctcgaac atcggcatgg gccagatgaa
     1501 ggcggccgat tacgtgctgg tgcccgacat cgccaacagc aattcgaatg ccagcggcaa
     1561 ccggatcggc gggctgctcg gcggattcct gccgcacggc atcggcgcag tggtgggcgg
     1621 gatcagcatt tccaagaaga ccgccgacgt ggtgctgacc ctgacggaca tccgttcgac
     1681 cgaacaggtc gcgctcgaac agggccacgc caacaagacc gacctcggct gggccggcgg
     1741 cggcggtggc ggcttctggg gcggcttcgc cgcaggcggc gccagcggct acgccaatac
     1801 cgaaatcggc caggtgatcg cgatggcgta cctcgacgcg tacaccaaaa tggtggccga
     1861 cctccagcac aatgccccga acgcgcaggc cgacaacgtg cagcaggcgg tgcggctcac
     1921 cgaagagagc aagctgtatg cggacgcgaa cctgcattcg tcggtggtgc gcaccttgaa
     1981 gcccggcatg atgctgtatc cgaccggcga caaggtcggc atctggtgga aggtgaccga
     2041 cgaactgggc aacattggct gggtgttgtc gagcaaggtg gaactggcgc actgaaccgc
     2101 gcaaggagga cgccgtgcgc cgattccggg cgtgaccggg ccggcgggcc gtgcccgccg
     2161 tacacgtacg gaacgcttac gccgtgccgg ccgcgggttg ttctttggac gcgatgtcgc
     2221 gcaatgcgcc ttcgaacgtc tccggcggct ggccgccggg gatcacccac ttgccgttga
     2281 tgatcgtggc cgggacggaa tggatgccgg cctgctggaa gcgctgttcc agcgtgcgca
     2341 cttcttcggc ataggcgtcg ctgtccagga cgccgcgcgc ctgcgcggca tcgagcccga
     2401 cgtcggttgc gatccgtacc agcacctggc ggtcggcgac gttttcctgg tcggtgaaat
     2461 acgcgcggaa cagcgcctgc ttcaactcga ccgcgcgctc gcgatcctgg atccccgccc
     2521 agtgcagcaa gcgatgggca tcgaaggtgt tccaggcgcg actgccgcgg ccggtattga
     2581 tcgcgacgcc cagcggcgca ccgcggtccc ggatcaccct tcgattttcc tcgagctggg
     2641 cctcgccgat gccgtacttc tgcatcaggc gctggtcggc gtcctcgcct tccaatggca
     2701 tctgcgggtc gagttcgaac ggttgcaggt agatgtccgc ctgcacggtt tcatccagcg
     2761 ccgccagcgc ccgctgcagg ctggcgagtc cgatcgcaca ccagggacag gcgacgtcgg
     2821 atacgaaatc gatgcgtaca cgggcggggg aagtggtcat cgggtcacca cggaaggaac
     2881 atgcacacta cgtggaggcg ttcgcgcgct tctcaatcgc gcatgctgtc ggacccgctc
     2941 ccgagccgcc catggatcgc cgccgcacgg aatatggtca cggccacggt catggcagcc
     3001 ggcacttgcg cggcgcccgc cgctcgatac actgacgggt gggatcgggg agcatatgga
     3061 catggacgag gacaacgccg tcgcggtcga caccagttcc cttgccgcca agtcgccgcc
     3121 gcaccaggcc cagcgcctgg cggcgctgcg ttcctacgac atcctcgaca cgccggtcga
     3181 gggcgctttc gaagacatca cctgcatcgc gagcctggtc tgccgggcgc cgatcgcagc
     3241 cgtcaatctc gtcgacgaga atcggcaatg gttcaaatcg gaggtcgggc tcggcgtccg
     3301 cgagatgccc ctggacgcgt cgttctgcgc gcacgcactg ttgcagaacg agttcctgga
     3361 agtccccgac atcgagctcg atccgcgctt ccgcgacaat ccgctggtgg tggatggtcc
     3421 gcgcctgcgc ttctacgccg gcgcgctgat ccgcaccgcg gaaggcctcg cgctgggaac
     3481 agtgtgcgtg atggacagcc gtccacgccg gctcgacgac gagcagcggc gcgtcctgcg
     3541 ggcgctggcg cggcaggcca tgacccaact cgagctgcgc cgcaccatga tcgtcgccgc
     3601 acgcgcgcac cgctaccgcg gtcggctgat ggcgatcgcc ggccatgacc tcaagcaacc
     3661 cctgaccacg atgacgatgg tgctcgacat gctgccggcc gcgacgccgg cggaccgccg
     3721 gcgcatcgaa gtggcgaagg aggccgcggc acggctcagg gaagaactcg acgggttgtc
     3781 ccgcgcgtcg cggctggaag aagaaacgga tgcgatcgaa ctggagccgt tgcaggtcga
     3841 cgaactgatc gccgaagtcg ccgacaactg gttgcgcgcg gcaaaggaca aaggcctcgg
     3901 cctgcaggtg cgggtgatcg atgccgaggt tcgcacccat gcgggcatgc tgcgcacgat
     3961 gctcgacagc ctggtcggca acgccatcaa gtacacgtcc cagggctcgg tcaccatcgc
     4021 ctgccgcgaa gagggcggcg acctgtggat cgacgtcgcc gacaccggca agggcattcc
     4081 cgagggccgc cgcgacgaga tgttcaccgc gttccgccag ctcgatccgg acacggacgg
     4141 cctggggctg ggactgtcga tcgtcaagag cacggccgac atgctcggat accggctgag
     4201 cctcgagtgc ggggaaggac ggggcgcgac cttcagcgtc tgcctgcccc ggcaggcgcc
     4261 cgtccaccgc tgactgccgt cccaaaggct cggcgcgcag ggatcgcgcg taccgcgcga
     4321 caaccacatg gatgaaggca tggccgacca cccgcgcaag gatccctcga acgaaaacaa
     4381 gcccggcggt ggcgaagagc cggacggctc cttccggctg accgagcgcg agaaggacga
     4441 agtcgaggaa aacctcccgc cgcgcgtgca ggtcctgcac gaggccatcc gcgtcgaagg
     4501 caagaccgaa ctcgggcgca gcgtcgcggc actgagctgg tcgtcactgg ccgcgggcct
     4561 gtcgatgggc ttctcgatgt tgatcccggc cttgctggcg gtgcatctgg tgggtgtcgc
     4621 gggcggcttc ctgatcgtga agctcggcta cagcatcggc ttcctgatcg tggtgtcggc
     4681 acgccagcaa ctcttcaccg aaaacaccct gaccgcggtg ctgccgttca tgacccgtcc
     4741 gcactggccg gaattcgggc ggctgctgcg gctgtggggc atcgtgctgg tcggcaacct
     4801 ggtgggtgtg gcgctgttcg cctgcggcat ggtctacatg cacgtgctgg atgccgcgac
     4861 gcacgccgcg ctggtgtcgc aggccgaaga ggtgatgcgc aacaccgtcc tccagatgtt
     4921 caccaagggc atcatcgccg gctggctgct cggcacgatg gtgtggctta ttccggcgct
     4981 gccgcacgcc aaggtgatcg tgatcgtggt gatgacgtgg ctgatcggcc tcggcaactt
     5041 cacccacgtg atagcgggtt cgaccgaagt gctgtacctc gtgtggcgcg gcgacctgcc
     5101 cgcggcggac tacatcgtgc ggttcatgct gccgacgctc gcgggcaaca tcgtgggcgg
     5161 cagtttcatc ttcggcctga tcagtcacgc ccaggtacgc agcgacgaca gctagcgggc
     5221 ggccggcgga cgggcacgcc tcttcctgtc tttcccgggt gcgtggtgtt cggacgcgat
     5281 ggcggtggcg acgtcgcggg gataggtttg catcgcgcgc agcatcacga aacccgccgc
     5341 ggccagcgcc accagcatca gcaggaacgc catgtcgagc ccgtgcccgg agccggcgcc
     5401 cgcgggtgag cgaccggcac tggtgctgcc gcccccgaac acgccggaca cccagccgaa
     5461 caagggcggg gcggctgcct ggaacgcgta cagcagggtg gaacgcacgc cttcggcgcg
     5521 cccccacaat cgcgaatgca tgatgtcgag ccgtgccgaa tcgaccgccg gattggcgcc
     5581 gccgaggccg ccggcggcca cgaagaaaaa cggggcggcg aaaatcaggc tgggcgcaag
     5641 cagaccgccc gcgagaccga gcgtcgcgac gaagtaggcc gccgcaccgg cgatgatgcg
     5701 accgcgcagg cgcccatggt cgatcatccg gtcggccgcc cagccggcga ccagcacgcc
     5761 cgcgatcgcg ccgacgccca ccgcgacggt cacggtgctg gccatcgcct gctggagagc
     5821 aaagtgctcg cgcatgaaca cgatcgcgaa cgtgcgcagg ccggtgaaat agaaatagcc
     5881 gagtgccgag gcgatgatca gcacccgata ggtgggaatc gacagcacgt agcggaccgc
     5941 gcgccacagc gacatctttt cggggtcctc cttgaggatc cgctcccgcc gcggatcgat
     6001 gccgcgctca tcgacgatct cgtgcacggg atgggtttct tccgcgtcgc cgtcctgcgt
     6061 ctcgtcctcc atccgcccct ggccaccgcg cttgggctcg ggcagcttgc gcgcaatcac
     6121 caccgcaagc accaggccga gtcccgccag gatgaagaac ggcacccgcc atgatgcgat
     6181 cgccgcgaca ttgccggaaa tcaggaaccc gaacgccacg ccgatcaatt cgccggccag
     6241 gatgtagctg tagatcctgg cgcgctcggc agggtggaag aagtcgccga ccagcgacgc
     6301 gatcgcgggc gatgccatcg ccaccaccgc gccgagtccc agccgggata ccagcatcat
     6361 cgggtagctg atcgcggcgc cgctcgcgat catcgcgagc gaccacaatc cgattccgat
     6421 caccagcagg cggccgcggt gcatgcggtc caccagcatg ccgaacggca gcgtcgcgaa
     6481 cgcgccgatc gcggtggaaa ccgtcaccag aaggcccacc tcgacgttgc cgatgtgcag
     6541 gtcgtgctcg agctgggcgg cgacggcgcc gatcgtggcc ttgtcggccg cgtccagcgc
     6601 cagcacgcag gccagcagcg cgatcacctt cagccgcttg cggccgccga cgatgtgctc
     6661 gagcggttcc tcgacatcgc gccggaacca gcgtcccaac tccacggccg cgatgacggg
     6721 tgccgattcc ggtgtctggc tgtcgtcttc cgcttccatg gctgcgcacc gggttcgagg
     6781 ctttccgtgc gcgagcactc tgccatatag tgcgtgtatg gcacgttcac gccccggtcg
     6841 ccaaggacag gctgccgcct cgcggaggcg actgccggtg cggttcggtg cagtcgcgat
     6901 ggcaagccat tgatggtcac tgcggcgcgc gctgcgcgaa agtccgcgac ccccgcgatc
     6961 tggttgctgg ccttcatgat ggcggccggc ccgttcggcg acaccgagta cacgccggcc
     7021 atgcccgcta tggccaaggc gctgcacgcc gactacggga tgatccagtt caccatggcc
     7081 gcgtacctgg cggcctcggc ggtgagccaa ctggtattcg ggccggcctc ggaccgctgg
     7141 ggtcgccggc ccgtgatgtt gttcggcgcg gtggtgctct cggcgggcgc actcctgtgc
     7201 atgctcagct tctccatctg gccgctgatc ggcggacgct tcatccaggg cgtcggcgct
     7261 tgcgcgggcg gcgtcatcgc cgatgcggtg gtgcgcgatc atttctccgc gaagcggcga
     7321 cagcgcgtgt acgccaagat caatgcggcg ttcgcggtgg cgccggcggt cgggccggtt
     7381 gccggcacct acatcgcgca cgcgttcggc tggcacgcga atttcgcgct gctgctcgcg
     7441 gtatcgctcg cgctcacata ctcagtctgg cgttggttgc cggaaaccct gcgcgagccg
     7501 gacccgcggg cgctcgaacg caagcggctg tggagcaacg cacgcgaggt gctcggcacc
     7561 cgcggtttcc tgttctacgt ggcgatgggg ggcttgtgcg tcggcgtggt gtacgcggcg
     7621 ttgattggcg cgcccgatct cgtgatcaac gtgctgcacc agggcagcgg cgcgatcgtg
     7681 gtggtcgcga tcgcgatcct cgtcgcgttc gtgatcggcg cgggggcgtg cgcatggctg
     7741 acaccgcggc tgcctcacct cgcgatcatc ggcggcggcc tggcgctggt cctcgccggg
     7801 tcgatcctgt tgatgctggt cgcgctgttc gcggacggca agggcaacct cgcgacctac
     7861 ctggtgccga tcggaatctc gttcatcggg gtcgggctca cggtgccggt gagcaccgcg
     7921 aaggccatgg cgccgttcgg cgacaacgcc ggcgccgcat cctcggtgct cggcttcctg
     7981 cggatgggca tcgccgcact cggcacgatc gcgatgagcg cgctgcacat gggcagcgtg
     8041 tatgacatcc ctatcgtttt cctcgcgctg tcggtgacgg ccgtggtgct gttctgcacc
     8101 tacctcgggt tgcgtggcaa gccgccggag gccgccacgc attgagcttg tggctgcccg
     8161 gcgagtgtgc caccgcgcaa tggagggcgc cgcgcgcatc accgcgtcgc gcggttcctg
     8221 cgcgcgcaga tgtagctcga ccgcgttccc tgccgtgacg gcgcggggcg aagccgcgca
     8281 gcggtttccg ccgatttttc aggagcggcg cccgggtccg cggcgtagcg gctgcgccga
     8341 ttccgcccta ccttccaccc gcggcctgcg cccaatctcg cagttgcgcc gcgaacttgc
     8401 ggctcgcctc ggcgagggct ttcgcgccgc cctgggcatc cggcgtcgcc gccgggactt
     8461 cgatatggaa acgtttttgc gcgatcacgt tgtcgtcgtg gttgtcgatc agcgtcgccg
     8521 tcgcgtcgag cacgcccttg ctttggccag gttccgcaaa ggcctggctg aagtcgtcga
     8581 gcctgagctg caggctcgcg tcggccgtcg ccggattgcg cggtccaatc accgcccgcc
     8641 agccgccgcc ggcgagggcg cgccgcagga tcggttcaag caggctcgcg ggcggcgaaa
     8701 tccagtccga ttgcccgtag gcggcaaggc ggccgtcgtt ccggtaatca agccggtaga
     8761 acatcgcgtt gccggaaagc cagtcgggca ccgcgatctg cgaaacctgc aggatctttc
     8821 cgcccgcgtt ggctgcaccg gaatcggtcg tgggtaccga tgccgttgtg ccgaggccgt
     8881 agtggccggt gaactgcgcc ttggtcgaat ggacgcaccc gccgaggaac agcaggcacg
     8941 caaccagcag caccgttgcc gggaagcgct cgcggcaggg cggattccct ccgccacgta
     9001 tccggtcggc aggcagtgcc cgccctgcga agcgtgtcgg gggcttgcta ctcatgaccg
     9061 tcaccatgct tgtcgttgcc ctggaaaccg ggctcgccgg ggccgggcgg cggtgcggcg
     9121 gggccgaaga tcaggctctg cggtttggcc ttcagctcac ggatcagctc ttcgagctgc
     9181 cgcgatgtgc gcaacaggct ctggctgagt gcctcgacgt cgggcgccgt ttgccggtcc
     9241 agcttgcgac ccaggtgctg gatcgtgtcc gcggcctggc cggcctgctt gaccggacca
     9301 cgtgcttcca ccgcgatctt gttggcattg gcgagcaacg cgtggctctc gtcgaggctc
     9361 ttctgcgcgc tctccaccag cgcgggcatc cgtttcatcg ccggcatcaa ctccgtttcg
     9421 atctgcacga gtttcgcggt cgccgcgtcg atctgccgga tcgaggcgcc gatgtgttcg
     9481 cggttttcgt cgttgagcac tttcctcgcg ttgtcgagca cggcctggag gtcctccatg
     9541 ctctgcttgg ccgaatcctc gagttgcgcg agcaggccct tgcgcagcgg aatttcggcg
     9601 ggatgcttgt cgctggtggc gagcagctcg cggcttccct tgcccagctt gagttccagc
     9661 gtctggccac cggtgatgcc gtgcatcgcg agcaccgcat acgtcgcatg ggtgatgtag
     9721 gtatcgcgct ggacgttgaa gtccacgatc acccgcgatc ggtcctcggg gtcgaagctg
     9781 atccgttgca cgtgcccaac ctcgatcccc ttgaactcga cccgcgattg cggggcgagg
     9841 ccggccacgg actgcctggt gacgatccgg tacgccagcg gttcgctggg ccccgacgtc
     9901 agccacaaga agatcagtgc cgcgacgatc gcgaacacga cgaggaagca gacggcgatg
     9961 atggcgtggg cgcggttttc catcggtcag gcaccgttct cgtggttcgc gaggtcgcga
    10021 agcggatcgt cattgcgccg ggagcggaaa aaatcccgga tgaacggatg gtcgaaccgc
    10081 gagacttcgc gcaaggtgcc gaccgtgatc agtcggccct cctcgagtac cgcgatccgg
    10141 tcgctcagcg tcgccatgct gtagaggtcg tgggtgacca tcatcaccgt gaggttcagc
    10201 tccttgtgga gcgcgccgag caaggcgtcg aactcggcgg aggatgcggg atccaagccg
    10261 gtggtgggtt cgtcgaggaa cagcagttcg gcctccagca tcagcgcccg tgcaagcgcc
    10321 gcgcgcttga gcatgccgcc cgacaactgc gccgggcgct tccaggcgtc ctcgggtttc
    10381 aggcccacca tgtacagctt gagcgccacg ctgtcgcgca gcatgttctc gtcgaccgac
    10441 cagccttcct tgcgcagttc gcgcatcggg aaggcgacgt tgtcgaacac attgaaggcg
    10501 ctgaacagtg caccctgctg gaacagaacg ccccagtgcc gccgcagcag gcgcgcctcc
    10561 tcgccgtcga gttcacccac cggggaaccg agcaccctga tcgaacccgc atcgggtttc
    10621 acgaggccga tcacttgttg cagcaaggtg gacttgcccg agccggacgt gccgatcacc
    10681 gacaggaact cgccgcggcg cacctccagg tcgagcccct tgtgcacctg ctggcggcca
    10741 tagcgcttgc acagtccccg gatctcgatc acggtctcgg cggctgcgga gtcggcggcg
    10801 acggcgttca cagcaggccc acgttggtca gtgccgcccc gagcgatgca tcgagcagca
    10861 ggatcatggt caggctggtg accaccgaag acgtggtgtt gcggctcatg ctctcggtgt
    10921 cgggcgacga gttcatcccg aagtacgaac caaccagcgc gatcaccagg ccgaacaaca
    10981 cgcccttgcc gaggccgatc cagaagttga cgatctgcac ctgctgcatc gcctcggcca
    11041 ggaacaaccg gaagttgatg ccgagttgcg cctgggcaac gagcgcgctt cccagcatcg
    11101 aggtgatgtc ggtccatacc accagcaacg gcatcgcgac ggcggccccg accacgcgtg
    11161 gcagcgcaag cctgaggctc ggtgcggaac cgagcgcctg cagcgcgttg tactcgtcgg
    11221 tgaggtgcat cgcgccgatg ctggcggtca tggccgagcc ggtgcgcccc gccatcagga
    11281 ggcccgtcac gaccgccccg agttcgcgcg gcacggcgat cgccatcatg ccgatcacca
    11341 tgtcggacgc actgaactgt tgcagcgcca gcccgagctg gacggtcatg accgcgccga
    11401 tcaggaagcc gatgctgccg agcagcacca tggccgtcgc gccgatctgg tagacgttgg
    11461 cggagatttc cttccacgga atgacccgtg gatggcgcag gcaataggcg atgtcgagca
    11521 tcaactggcc gaccagcagg aagatgcccg ccgcggtcgc gagcaccgcg atgattcccg
    11581 cgccgagccg atcgagccag tacagcgatc cggtgcgcgc cggcctgccg agcgccggca
    11641 tgtcctcgag ccgctggaac aggtggcgct gttcgtcgct gcaggcgagc tgggccggca
    11701 gccgcccctg ccaggcccgc cacaacacca gcgcgccggt gctgtcgagc gactcgacgt
    11761 gctgcaggtc ccagcagtag tcgccggggg cgccgagccg cctgaactgg cggagcaatc
    11821 gccggcgccg ttgcttgtcc accagcaggt tccacgatcc ggacagcgtg atgcgggctt
    11881 cgccctcgcg cgtccatgtg ggtccggatg cgggttttgc catgggcgga caatcctgtg
    11941 tcgcggttcg ggcagccgat ccgggacaag gtgcgacatc gagcgtgcag acggcgtgga
    12001 agcgttcgtt gagggcgcgt gaacgcgcga gcgggacgtg gacggggcag ggctgcgcag
    12061 gtagcgatcc cgtaagatga accgcaacct cgccacgcaa gggcatcgcc atgtcgtcca
    12121 tcgcgcttcg cgcaagccgc ctgctgctcg gcgcggtggc gttgttcctg gcggtacagg
    12181 cgtgggcggc cggccccgcg cccacggtgc gggtcgacgg tgggcgcctg tccggcatcc
    12241 acgacgcctc gaccgggctc gacgaattcc gcggcatccc gtttgccgca ccgcccgtcg
    12301 gtgcgctccg ctggaagcca ccgcaacccc tggcggggtg gacgggggtc cggcgcgccg
    12361 accacttcgc cccgcgctgc atgcagcggc cgctgttcgg cgacatggtg ttccgctcgg
    12421 acggcatcag cgaagactgc ctgtacctca acgtgtggac gccggcgcat cgctccggcg
    12481 ggaagcttcc ggtgctggtt tatttctacg gcggcggatt cctcgccggt gacggttcgg
    12541 agtatcgcta cgacggcgcg agcctggcca ggcgaggaat cgtcacggtc acggtgaact
    12601 accgcctcga cgtgttcgga ttcctggcgc tgccggcgct cgccgccgaa tccccgcacc
    12661 acgcgacggg caactacggg ttgctggacc aggtggcggc gttgcgctgg gtacgacgca
    12721 acatcgcggc cttcggcggt gatccagcca aggtcacgat cgggggggaa tcggccggtt
    12781 ccatgtcggt gtcggcgctg atggcatcgc cgctttcgaa agggctgatg caacgcgcga
    12841 tcggggaaag cggtgccgtg ctcggcaacc tcgggccgaa gccgctggcc gtggcggagc
    12901 gcgaaggcac cgatttcgta cggcatgtcg gcgcgggttc gctcgcgcag ttgcgggcga
    12961 tgccggcggc gaagctgatc gcggcgtgtg gcgacccggg catgcccgaa ttcggtccga
    13021 cggtcgacgg ttggttcctg ccgcgatcac ccgaactgat ctacgaggcc ggcgaacagg
    13081 cgcacatccc gttgctggtc ggctcgaatt cgggcgaggg cagttacacg gcgctgttga
    13141 aggacgcgcc gccgacccct gccaactatc gcgcggcggt gggacgggaa ttcggcacgc
    13201 acgcggacga ggtgctgaag ctgtaccccg gcgacagcga gcccgaagtg aaggcctccg
    13261 gcgacgccct ggccggcgac cgcttcatcg cgtattcgac ctggcgctgg atgcatctcc
    13321 agcgcaccac cggccacgca cccgtgtact actactggtt cgacaaggcg cgtccggcca
    13381 ggcgcgacgg cagcgccggt cccgacgagg cggccgtaca cagcggcgag atcgaatacg
    13441 cgctcggcaa cctcgcaacc aaccacgtgt atgcgtggac ggcggccgac cgcgaggctt
    13501 cggcgacgat ggaaggctac ttcgccaact tcatcaagac cggcaacccg aatggcgacg
    13561 acctgccgca ttggccggcg gcgacgcgca aggatggcgg cttgctgcgc caggtgatcg
    13621 gcgtgcacac gcggaccatc gtcgatcgca aggcgccacg ttacgaattc ctgcagcaca
    13681 tcgaggcggg cgcgcccctg taacaggagt gggcgcagct cggcgcgcta tgctgtccgg
    13741 atggtttccc gccgtttccg ccaggtcgat gtcttcggtt ccgccgcgta ccggggcaat
    13801 cccgtggcgg tggtcatcga tggcgaaggc ctcg
//
LOCUS       DASSNH010000027         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_228238, whole genome shotgun sequence.
ACCESSION   DASSNH010000027 DASSNH010000000
VERSION     DASSNH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228238"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFK00_00945"
     CDS             <1..444
                     /locus_tag="VFK00_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7591554.1"
                     /translation="ANAAQPPAMEADHTALSPGDLKWMPAPPVLPKGVQMAVLSGNPS
                     AEGFTIVRLKIPAHTTFAPHWHPTAESFTVLQGSLHFGMGDKVDKSAAKTLPTMGFAS
                     MPATHHHYVYTEREGAVIDLSFYGPFQIHYVNPADDPSKQSMASD"
     gene            520..>2133
                     /locus_tag="VFK00_00950"
     CDS             520..>2133
                     /locus_tag="VFK00_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HET7591555.1"
                     /translation="MPARQAPHFGAWLGTVLLTLASGAVLAAAPASTAASGALTQSER
                     DGHGRFGNAVTSADWRIDGGALANLVVSDQRHHVTIPVTDPFSLTMQDGSTLATGDFR
                     LQGTLHEVALPVDLNAARLAERLPGKAVEGRFLDRAGHVRIDWKLVQRDGSDYLREIV
                     TITALDRDAPITKVALFGADATDAIVVGSVDGSPVVSHDDWLGFEHPMSKADAWRGKV
                     KMWIERTLPLPEGQSVTYSAVIGVAHEGQMRRDFAAYIERERAHPYRPFLHYNSWYDI
                     GYFTPYTEAEALDRIDTFGRELVEKRHVRMDSFLFDDGWDDYNGSWHFSKAFPHGFVP
                     LCEAAKKYGAAPGVWLSPWGGYGKPRQERIANGRRAGYEIVDGGFALSGPKYWKRFHH
                     AVKALLDDDCINQFKLDGTGNVDSVYPGSRFDSDFAAAIQLISDIRADKPGTFINLTT
                     GTWASPFWLRYADSIWRDGYDHNFTGVGPKREQWITYRDEQTYRNIVEKGPLFPINSL
                     MLHGIIFAQHAVGLNTDPGHDFANEVHSYF"
BASE COUNT          393 a          694 c          687 g          359 t
ORIGIN      
        1 gcgaacgccg cgcagccgcc cgccatggaa gccgatcaca ccgcactctc gcccggcgac
       61 ctgaaatgga tgccggcgcc tccggtactg ccgaaaggcg tgcagatggc cgtattgagc
      121 ggcaacccat ccgccgaggg cttcaccatc gtgcggctca agatacctgc acacacgacc
      181 ttcgcaccgc actggcatcc gacggcggaa agtttcaccg tcctgcaggg gtcgctccac
      241 ttcggcatgg gcgacaaggt ggacaagtcc gccgccaaga cgctgcccac gatgggtttc
      301 gcctcgatgc ccgcgaccca ccatcactac gtctacaccg aacgcgaggg tgccgtgatc
      361 gacctcagct tctacggccc gttccagatc cactacgtga atcccgcgga cgacccgtcg
      421 aagcagtcga tggcatcgga ctgatccgat cgtgctgtag cgaaagaggc gatcaggcag
      481 aatgacccgt gccggatctc ccgcgcggag ggattctgca tgcctgccag gcaagcgccg
      541 cacttcggcg catggctcgg aactgtcctg ctgacgcttg cgtcaggcgc cgtgttggct
      601 gcggcgcccg cgtcgacggc tgcgtccggt gcattgaccc agagcgaacg cgacggccac
      661 ggccgattcg gcaatgcggt cacaagcgcc gactggcgca tcgacggcgg cgcgctggcg
      721 aacctggtgg tgagcgacca aaggcatcac gtgacgatcc ccgtcaccga tccgttttcg
      781 ctgacgatgc aagacggcag cacgctggct accggcgatt tccggctgca gggaaccttg
      841 catgaagtgg cattgccggt ggatctgaat gccgcacgcc tggccgaacg ccttccgggc
      901 aaggcggtgg aagggcgctt cctcgaccgc gccgggcacg tccggatcga ctggaaactg
      961 gtgcagcgcg acggttcgga ttacctgcgc gagatcgtca cgatcaccgc gctggaccgg
     1021 gatgcgccga tcacgaaagt ggcgctgttc ggcgccgacg caaccgatgc catcgtcgtg
     1081 ggctcggtcg acggctcgcc cgtggtgtcg cacgacgact ggctgggctt cgagcacccg
     1141 atgtccaagg ccgatgcctg gcgaggaaaa gtgaaaatgt ggatcgaacg cacgctgccg
     1201 ctgcccgagg gccagtcggt gacgtattcc gcggtgatcg gcgtcgccca cgaggggcag
     1261 atgcggcgcg atttcgccgc gtacatcgaa cgcgaacggg cgcatccgta ccggccgttc
     1321 ctgcactaca actcgtggta cgacatcggc tatttcacgc cctacaccga ggcggaggcg
     1381 ctggaccgca tcgatacgtt cggcagggag ctggtcgaga aacgccacgt caggatggat
     1441 tcgttcctgt tcgacgacgg ctgggacgac tacaacggaa gctggcattt ctcgaaggcg
     1501 ttcccgcacg gtttcgtgcc gctgtgcgag gcggcgaaga agtacggcgc cgcgcccggt
     1561 gtgtggctgt cgccgtgggg cggttacggc aagccacggc aggagcgcat cgccaatggc
     1621 cgcagggcgg gttacgagat cgtcgacggc ggtttcgcgc tgtccggccc gaagtactgg
     1681 aagcgcttcc accacgcggt gaaggcgctc ctcgacgacg actgcatcaa ccagttcaag
     1741 ctcgacggca ccggcaacgt cgacagcgtg tatccgggca gccgcttcga cagcgatttt
     1801 gcggccgcga tccagttgat ctcggacatc cgcgccgata agccgggcac cttcatcaac
     1861 ctcaccaccg ggacctgggc atcgccgttc tggctgcgct atgcggattc gatctggcgc
     1921 gacggttacg accacaactt caccggcgtg ggtcccaagc gcgagcagtg gatcacctat
     1981 cgcgacgagc agacctatcg caacatcgtg gagaaggggc cgctgttccc gatcaattcg
     2041 ctgatgctgc acgggatcat cttcgcccag cacgcggtgg ggttgaatac cgatcctggc
     2101 cacgacttcg ccaacgaggt gcattcctat ttc
//
LOCUS       DASSNH010000028        12183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_838538, whole genome shotgun sequence.
ACCESSION   DASSNH010000028 DASSNH010000000
VERSION     DASSNH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838538"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VFK00_00955"
     CDS             <1..1368
                     /locus_tag="VFK00_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7591556.1"
                     /translation="SFAVSNAIALPLTGFLSRRFGEVKLFVWATALFSLCSLLCGLAT
                     SMDMLVLFRVLQGAVAGPMYPITQSLLVSIYPREKRGMALAILGMVTVVAPIAGPVLG
                     GWITETYSWEWIFFINVPIGVFASTVVGAQLGKKVEQLRKARVDWVGLITLVLGVGSL
                     QIVLDKGNELDWFHSGMIVALAIISAVSLAVFLIWELTDDEPIVDLRLFRHRNFAAGT
                     LALVLAYAAFFSIGLLVPLWLQNTLGYTSIWAGLATAPIGIIPVLLTFWVGKYATRFD
                     LRWLAAFAFIVMGVTCFMRGRFNVDVDFYHVALAQLIQGLGVALFFMPILTILLSDLE
                     GPEVSEGSGLATFLRAVGGSFSASITTYIWTRGGVVNHANLGEHINLYEARVRDAIAA
                     TGGHLQTYAAGMDRVISQQAMQISFNQLFDALGFIFLGLVAVVWLAKPPFIKKGGPAA
                     TGGGH"
     gene            1633..3189
                     /locus_tag="VFK00_00960"
     CDS             1633..3189
                     /locus_tag="VFK00_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017115296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7591557.1"
                     /translation="MASNAATASQPVGEFRPASLPLATMGLSLGTFMQVLDMTIANVS
                     LPTIAGNLAASQDQSTWVITSFTVAQAITLPMTGFLSRRFGEVKIFIGAVLLFSLFSL
                     ACGFATSLSMLVMFRAMQGAVCGPMYPITQSLMVSIYPREKRGMALAIISMITVVAPI
                     VGPVAGGWITDSYSWRWIFFINVPIGIFAGFIVLAQMGKRTERLVRARIDWVGLATLI
                     LGVGALQIMLDKGNNLDWFHSTFIVTLAIVAAISLTLWVIWELTDEQPLVDLRLFRHR
                     NFAAGTLALVLAFALFFSVSLLLPLWLQNTLGYTATWSGLAAAPVGVIPVLLTYFVGK
                     YATKFDLRWLTAFSFAVMGLVCFRFGSFNTDVDFASVALTELIMGLGIALFFMPILTI
                     LLSDLEGQEIAEGSGSATFLRTVGASFAVSIVTYLWSRGSVVSHANLAEHINPFNGAV
                     RHSVAVAGGELQRYAEGINRVITQQATQISFNHLFDGIGVGFFILIAVVWLAKPPFVQ
                     RGGGPPASGH"
     gene            complement(3302..3688)
                     /locus_tag="VFK00_00965"
     CDS             complement(3302..3688)
                     /locus_tag="VFK00_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7591558.1"
                     /translation="MSRTILIVDDEFGIVEVLAAALSDHGYGIMQAYNGAQALARIAD
                     EVPDLVISDYMMPGGDGGALLATIAREFAAIPVILMSAVDEVSIKRAGISYTAFLRKP
                     FRIAEMLDAVRRLVGHGGDADNAGIH"
     gene            complement(3685..5190)
                     /locus_tag="VFK00_00970"
     CDS             complement(3685..5190)
                     /locus_tag="VFK00_00970"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET7591559.1"
                     /translation="MSSQAPPDGFARVPTGIPGLDQVLQGGLLDGGLYVVAGTPGSGK
                     TILANQFCFARAAAGSRSLFVTMLGESHGRMLRHLSTLDFFEPSHVPERVYYLSGAQA
                     IESNALGNLMALLRKEIAARDAGVLVLEGLFSSAAERPEQELRRFIYQLQAEVQAAGC
                     TALLTTDQLRPDYHPEYTLVDGIILLEQVARGSRSMRRLQVQKFRGSGFFAGKHDYEI
                     TEAGIAVYPRIESVYARPSDRDRSLGTRTPIGVAGIDRMIGGGVPSGTTTLVLGPSGV
                     GKTVLGLHYMCAATPEQPALLFSFYETPERLLMKARSVGLGLDKLIQQGTVEVVWQPP
                     GGDSMDHVAQRLLRVVEQRGVRRLFIDGMQGFEAATADPERLVRFFNTLFNELRVRDV
                     TSLYTGETQILIGSEVRSPVNGLSMLSENILLLRFVERRARIHRIFSVLKVRDSAIDS
                     ALREFHISSSGIDLASTSATADALYDKLQPPSRPGPPGFPAADPQPDRGDR"
     gene            complement(5168..6001)
                     /locus_tag="VFK00_00975"
     CDS             complement(5168..6001)
                     /locus_tag="VFK00_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7591560.1"
                     /translation="METEHRAGNEPGSGEPATHGDTREHTDLIARLRAEIRARDDFVA
                     IAAHELRNPMTSILGLVELLQSGARQDPESCSPKVRARLALLHEEVGGFIKRATVLLD
                     TTRIISGMLQLKPEWVDLSAIVESAVQRHGTRAVKMGSSINADVEGGLHGCWDALAIE
                     QIMDNLLSNAVKYGGPSEISVELADLGSAARFTVRDRGRGMTPAELDRIFSRFEQAVA
                     RRDSSGFGIGLWLVRGLVDAMRGSIDVRSEPGNGTSFEIILPKDVGSMNRNSDEQPST
                     S"
     gene            complement(6128..6892)
                     /locus_tag="VFK00_00980"
     CDS             complement(6128..6892)
                     /locus_tag="VFK00_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7591561.1"
                     /translation="MLERIEHERGILELRLARPPVNALDPELIATLKRSIEQAPNDGA
                     DALVLSGSPGLFSAGLDIPALLQLDRGAMRVFWNEFFGVCAVLARSPIPIVAAVTGHS
                     PAGGAVLAIFCDYRVMARGEFRIGLNEVQVGLTVPDCIQAALRRLVGPYRAERLLVAG
                     AMLDSEDALAQGLVDELTDIDHVTTRAIAWLEPLLQLPRQAMLATRNMARAGLAGLFD
                     DPACLPVEEFLDGWFATEAQTTLHALVERLRNKKPA"
     gene            complement(7027..7908)
                     /locus_tag="VFK00_00985"
     CDS             complement(7027..7908)
                     /locus_tag="VFK00_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET7591562.1"
                     /translation="MSDAPVTPSSFDYATAFSRTIGWMTSAEQPKLRSKRVAIAGLGG
                     VGGAHLLTLTRLGIEAFNLADLDRFGTENLNRQAGAFQSTIGQPKVDVVSRMARDINP
                     DLEINKFPNGVTFDNMEQFLSGVDLYVDGLDFFVLDIRAKVFAMCAERGIPAITAAPL
                     GMGAAVLAFLPGQTTFEEYFRLEGQPMNEQLLRFMVGLAPAVLHQSYLVDPSAVDLVN
                     HRGPSTPMACEICAGIAGTQALKILLGRGKVYAAPHGLHFDAYRNKLVHTWRPGGNNN
                     PLQRLLLSVARRRFMRQ"
     gene            complement(8131..9075)
                     /locus_tag="VFK00_00990"
     CDS             complement(8131..9075)
                     /locus_tag="VFK00_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448552.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7591563.1"
                     /translation="MLLALYGQGKATRCRCLWSLTEWNQVKEKSMLNKKILGAALVGA
                     LALGASAAAFATPVNVGGVVFDPDSPADLSIDALNFREGSISQVGDVLTGYGRIGSIN
                     GTDPDEFCPGCDLTFTFQYTVQSIDNVGGNTQIVFNAGTIQFYVDDTMSFNVLDPTTS
                     GIGTEWLTMTGHPGSFTGYTLPDQAGGELYSTVLGTIAEPQAGSNGIGVLDATGGPVA
                     GYFDTNTVQDGLGGFADFSINSSFLVKLIDGCTAGSPSPDPNDICHYPISGTAFVIGN
                     SMLPVSVPEPGELGLLGLGLGALGFMVRRRRKEASRRA"
     gene            complement(9322..10431)
                     /locus_tag="VFK00_00995"
     CDS             complement(9322..10431)
                     /locus_tag="VFK00_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591564.1"
                     /translation="MHPYQPTLDEAKGVDQDIKAGILGAGIAAPSADNSQPWRFAWSG
                     DDLVVRIDRDRSGGISDTRYVLSDLAIGACLENMLLHARSRGYGADLETFPQQDDPLC
                     VARIRWRRDATDRPEPLATAIGQRHTDRRFPWRGPITADTRAGLDAQARLIPGARLWW
                     PQTPRERKAAQGLIRQAEALRFKSPALHAELFSSIRFSAGWHTACEEGLPPAALAVEP
                     PLRPVFQAMRRPGVMASFNRLGAASMLGWRSAWVPIRLSPGLCLIVVPSTARADVLSG
                     GRALQRVWLQATLDGLSAQPYAAPGVLGLGFIPLEPAFQQARSRLASALGDLTGTGTG
                     LVFLRLGYARSPPAGRSGRRPLASFGDFSEAVARS"
     gene            11486..>12183
                     /locus_tag="VFK00_01000"
     CDS             11486..>12183
                     /locus_tag="VFK00_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591565.1"
                     /translation="MESAWGLVYRRYSEMGLIDENPFRIHASPTGVGEHACVIWGPEG
                     PETGYTMTLFRDNSTGLALDSVYPRDLEGMRRAGRGLLEVGMLADRRRCASRGMKALF
                     SMMRWAVHYGLQCGLTDVVIGVHPHHAPFYMRCYGFEQFGEETSYPLVRNHPVVGLRL
                     RLHEGLAKDVLPRGLADARDNPVPRSAFANRFRFEPAQLGGSVIANYMKARYGIGLDR
                     VTGWTQPAAVAADRP"
BASE COUNT         2113 a         3841 c         3933 g         2296 t
ORIGIN      
        1 tcgttcgcgg tgtcgaacgc gatcgccttg ccgttgacgg gattcctgtc gcggcgcttc
       61 ggcgaagtga agctgttcgt atgggcgacc gcgctgttct cgctgtgctc gctgctgtgc
      121 ggccttgcta ccagcatgga catgctggtg ttgttccggg tgctgcaagg cgcggtcgcg
      181 gggcccatgt atccgatcac gcagagcctg ctggtatcga tctacccccg cgagaagcgc
      241 ggcatggcac tggcgatcct cggcatggtc acggtagttg cgccgatcgc gggcccggtg
      301 ctgggcggct ggatcacgga aacctattcg tgggaatgga tcttcttcat caacgtgccg
      361 atcggcgtgt tcgcgagcac ggtggtcggc gcgcaactgg gcaagaaggt cgaacagttg
      421 cgcaaggcac gggtcgactg ggtggggctc atcacgctgg tcctcggcgt gggttcgctg
      481 cagatcgtgc tggacaaggg caacgagctc gactggttcc attcggggat gatcgtggcg
      541 ctcgcgatca tttcggcggt ttcgctggcc gtgttcctga tctgggaact gaccgacgac
      601 gaaccgatcg tggacctgcg cctgttccga catcgcaact tcgcggcggg cacgctggcg
      661 ctggtgctgg cctacgcggc attcttctcg atcggcctgc tggtgccgtt gtggctgcag
      721 aacaccttgg gctacacgtc gatctgggcg gggcttgcga ccgcgccgat cgggataatc
      781 ccggtgctgc tgacgttctg ggtgggcaag tacgccacgc gcttcgattt gcgctggctg
      841 gcggcattcg cgttcatcgt gatgggggtt acctgcttca tgcgcgggcg cttcaacgtg
      901 gacgtcgatt tctaccatgt cgcgctggcg cagttgatcc agggcctcgg cgtggcgttg
      961 ttcttcatgc cgatcctgac catcctgctg tcggatctcg agggaccgga agtgtcggaa
     1021 ggctcggggc tcgcgacgtt cctgcgcgca gtgggcggca gtttctcggc ttcgatcacc
     1081 acctacatct ggacacgcgg cggcgtggtg aaccacgcca acctgggcga acacatcaac
     1141 ctgtacgagg cgcgggtccg tgatgcgatc gcggccaccg gcgggcacct tcagacctac
     1201 gccgccggca tggaccgggt gatcagccag caggcgatgc agatctcgtt caaccagttg
     1261 ttcgacgccc tgggcttcat cttcctcggc ctggtcgccg tggtctggct cgccaaaccg
     1321 ccgttcatca agaagggcgg cccggcggcg accggaggag gacattgatt cgcgatcttc
     1381 cgtggtccaa atctttttga cgcggatgaa cgcggatcaa agcggatgag tacagctttc
     1441 tgttctttgg ccctggagcc gcgtcaggtt ttgaaagaac atttggcaag gataaacccg
     1501 ggaaaaagga tcattgtctt ggggtctatc cgctttgatc cgcgtccgaa aaggtattgc
     1561 cttgtttccg ctctgatccg agtcgacgct tttgcaccgc ttggccgctc cgcctgagag
     1621 caaaaggaat tcgtggcctc gaacgctgcc accgcaagcc agccagtggg tgaattccgg
     1681 cccgccagcc tgccgctggc gacgatgggc ctgtcgctcg ggaccttcat gcaggtcctc
     1741 gacatgacga tcgcgaacgt ttcgctgccg acgattgcgg gcaatctcgc ggccagccag
     1801 gaccagtcca cctgggtcat cacctcgttc accgtggctc aggcgatcac gctgccgatg
     1861 acgggtttcc tttcccgccg cttcggcgag gtgaagatct tcatcggggc cgtgctgctg
     1921 ttctcgctgt tttcactggc ctgcggcttc gccaccagcc tttcgatgct ggtgatgttc
     1981 cgcgcaatgc agggcgccgt gtgcggtccg atgtatccga ttacccagag cctgatggtg
     2041 tcgatctatc cgcgcgagaa gcgcggcatg gcgctggcga tcatcagcat gatcacggtc
     2101 gtcgcgccca tcgtggggcc agtcgcgggc ggctggatca ccgactcgta ttcctggcgg
     2161 tggatattct tcatcaacgt gccgatcggc atcttcgccg gtttcatcgt gctggcgcag
     2221 atgggcaagc gcaccgagcg cctggtgcgg gcgcgcatcg actgggttgg gctggcgacg
     2281 ttgatcctgg gcgtcggcgc gttgcagatc atgctcgaca agggcaacaa ccttgactgg
     2341 ttccattcca cgttcatcgt gacgctcgcg atcgtcgcgg caatttcact gacgctctgg
     2401 gtgatctggg agctgaccga cgagcagcca ctcgtggacc tgcgcctgtt ccggcaccgc
     2461 aatttcgcgg ccggcaccct ggcgctggtg ctggcgttcg cgctgttttt ttccgtatcg
     2521 ctgctgctgc cgctgtggct gcagaacacg ctcggttaca ccgcgacgtg gtccggcctc
     2581 gccgccgcgc ccgtgggcgt catcccggtc ctgctgacgt acttcgtggg caagtacgcc
     2641 acaaagttcg atctgcgctg gctgacggcg ttttcgttcg cggtgatggg cctggtctgc
     2701 ttcaggttcg ggagcttcaa caccgacgtg gatttcgcca gcgtcgcgct gaccgaactg
     2761 atcatgggcc tcggcatcgc actgtttttc atgccgatcc tgaccatcct gctctccgac
     2821 ctcgaaggcc aggagatcgc ggaggggtcg ggctcggcga ccttcctgcg cacggtgggc
     2881 gcgagcttcg ccgtgtcgat cgtgacgtac ctctggtccc gcggcagcgt ggtcagccac
     2941 gccaatcttg ccgaacacat caatccgttc aacggtgcgg tccgccacag cgtggccgtg
     3001 gcgggcgggg agctccagcg ctacgccgag gggatcaatc gggtgatcac ccagcaggcc
     3061 acccagattt ccttcaacca cttgttcgat ggcatcgggg tcggattctt catcctgatc
     3121 gcggtggtgt ggctcgccaa gccgccgttc gtgcagcgcg ggggcggccc gcccgcaagc
     3181 gggcattaat cagcccgtcg cttgcgggtc ccggcaacac atcgtcaacc ttgcgccacc
     3241 ttcacggtca gcttgcacgg ctggaacgag acgcatggcc gaagcggcac ctgctggtcg
     3301 atcaatggat gccggcgtta tctgcatccc cgccgtgtcc gacgagcctc cgcaccgcgt
     3361 ccagcatttc cgcgatcctg aatggcttgc gaagaaaggc cgtgtagctg atgccggccc
     3421 gcttgatcga cacctcgtcg acggcgctca tcaggatcac cgggatggcg gcaaactcac
     3481 gggcaatggt ggcgagcaat gctccgccat ccccgcccgg catcatgtaa tcggaaatga
     3541 ccaggtccgg aacttcgtcg gcgatgcggg caagtgcctg ggcgccgttg tacgcctgca
     3601 tgatcccata gccgtgatcg ctcaaggcgg cagcaagcac ctccacgatg ccgaattcgt
     3661 catccacgat gagaatggtg cggctcatct gtcgccgcgg tccggctgcg ggtccgccgc
     3721 agggaaccca ggcggcccgg gcctggaggg tggctgcaac ttgtcataca gggcatctgc
     3781 cgtggccgag gtggacgcga gatcgatgcc ggatgaactg atgtggaatt cgcggagcgc
     3841 gctatcgatg gcactgtccc gcactttcag gaccgagaaa atccggtgta tgcgcgcgcg
     3901 cctttccacg aatcgcagca gcaaaatgtt ttccgagagc atcgaaaggc cgttcacggg
     3961 ggaacgcact tcgctgccga tgagtatttg tgtctctccc gtgtaaagag aggtcacgtc
     4021 gcgcacgcgc aactcattga acagggtgtt gaagaagcgg accagccgct ctggatcggc
     4081 ggtggcagcc tcgaagcctt gcatgccgtc gatgaacagg cgccgcacac cgcgctgctc
     4141 caccaccctc aggagccgct gcgcgacatg atccatcgag tctccgccag gcggctgcca
     4201 gacgacctcg accgttcctt gttggatcag cttgtcgagg ccaagtccga cgcttctggc
     4261 tttcatcaga agccgttcag gggtttcgta aaaggagaac agcaacgccg gttgttccgg
     4321 agtcgccgca cacatgtagt gcagccccaa tacggttttg ccgacccccg aggggccgag
     4381 aaccagcgtg gtcgtacccg aaggcacgcc gcccccgatc atgcggtcaa tgccggccac
     4441 gccgatgggc gtgcgggtgc cgaggctgcg gtcgcgatcg gaaggccgcg catagacgga
     4501 ctcgatcctg ggatacaccg caatgcccgc ttcggtgatc tcgtaatcgt gcttgccggc
     4561 gaagaagccg ctgccccgga acttctgcac ctgcaagcgc cgcatgcttc gcgacccgcg
     4621 cgcgacctgt tccagaagga tgatgccatc gaccagcgtg tattcggggt gataatcggg
     4681 ccgtagctgg tcagtcgtca gcaacgccgt gcagccagct gcttgcactt ccgcctgcaa
     4741 ttgatagatg aagcgccgca gttcctgttc cggccgctcg gcggcgctcg aaaacagccc
     4801 ctccagcacg agcacgcccg catcgcgggc cgcgatctcc ttccgcagca gtgccatgag
     4861 gttgcccaag gcgttcgatt cgatcgcttg ggctccgctg aggtaataga cgcgttcggg
     4921 tacgtggctc ggttcgaaaa agtccagggt cgacaggtgc cgcagcatgc gcccgtggga
     4981 ttcgcccagc atggtcacga acaggcttcg gcttccggcc gctgcacgtg cgaagcagaa
     5041 ttggttggcg aggatcgtct tcccggaccc cggggtgccg gcgacaacgt acagtccgcc
     5101 gtccagaagg ccgccctgca gcacctggtc caggccaggt atgccggtgg gtacgcgggc
     5161 aaatccgtca ggaggtgctt ggctgctcat cggagttcct gttcatggag ccgacgtcct
     5221 tgggcaatat gatctcgaag gaggtgccgt tgccgggttc gctgcgtacg tcgatgcttc
     5281 cgcgcatggc gtcgacaagc ccgcgcacca gccaaagccc tatgccgaac ccgctgctgt
     5341 cacgacgtgc gaccgcctgt tcgaaccggg aaaagatccg atcgagttca gcgggcgtca
     5401 tgccccgacc acggtccctt accgtgaatc gtgcagcact gccgagatcg gcgagctcga
     5461 cgctgatctc cgacggcccg ccatacttca cggcgttgga cagaagattg tccatgatct
     5521 gttcgattgc gagcgcgtcc cagcatccgt gcagcccgcc ctcgacgtcc gcattgatgc
     5581 tgctgcccat cttcaccgct cgggttccgt gccgttggac tgcagactcg acgattgcgg
     5641 acaaatcgac ccactccggc ttgagttgca acattcccga aatgatcctc gtggtgtcga
     5701 ggagcacggt cgctcgcttg atgaacccgc cgacttcttc gtgcagcaac gcgagccggg
     5761 cgcggacctt cggcgagcag ctttcgggat cttgccgtgc ccccgactgc agtagctcca
     5821 ccaatccaag gatcgacgtc atcggattgc gcagctcatg cgcggcgatg gcaacgaagt
     5881 catcgcgggc ccggatttct gcccgaagcc gggcgatcag atcggtatgc tcgcgggtgt
     5941 ccccgtgcgt ggccggctcc ccgctgccgg gttcgttgcc cgcgcgatgt tcagtttcca
     6001 agcaggtccg tcccgtcttc agcagctgcg agattcgacg atagagcttg ctcgttgagg
     6061 cggcattatg tcaacggaga atgtattgca agagcggtgg catccgggag gcagggaggt
     6121 ctgccatcta agccggcttc ttgttgcgca ggcgctcgac cagtgcatgc agggtggttt
     6181 gcgcctcggt tgcgaaccag ccgtcaagga attcctcgac ggggaggcag gcgggatcgt
     6241 cgaacaggcc ggcgaggccg gcgcgggcca tattgcgggt cgccagcatg gcctgccgcg
     6301 gcaattgcaa cagcggttcc agccatgcga tcgcgcgtgt ggtgacatgg tcgatgtcgg
     6361 tcagttcatc gaccaggcct tgcgccagcg cgtcctcgga atcgagcatc gccccggcca
     6421 cgagcagccg ctcggcacgg tacggaccga ccagtcgtcg cagcgcggcc tggatgcagt
     6481 cgggtacggt cagcccgacc tgcacctcgt tgagcccgat ccggaattcg ccccgcgcca
     6541 tgacacggta atcgcagaag atcgcgagca ccgcgccgcc ggcggggcta tggccggtga
     6601 cggcggcaac gatcggaatc ggtgaccgcg ccagaactgc gcagacaccg aagaactcgt
     6661 tccagaacac gcgcatcgcg ccacggtcga gttgcagcag ggcaggaatg tcgagcccag
     6721 ccgagaagag gccgggcgag cccgacagca ccagcgcgtc ggcgccgtca ttcggggcct
     6781 gttcgattga tcgcttcagc gtggcgatga gttcgggatc cagtgcgttg accggcggcc
     6841 gcgcgaggcg cagctcgagg atgccgcgtt cgtgttcgat tcgttcgagc atgttgattc
     6901 cgaaaggtcc agcgcaatgg tcaaaattgg tcccgacttg ggccgggaga cggccgaatg
     6961 gataggcttg ccggccgcgg gtcctgagtc cagaagcatg ggaccagcgc agcgcgtgcc
     7021 gcggtgctac tgccgcatga aacggcggcg cgccaccgac agcagcagcc tctgcagcgg
     7081 gttgttgttg ccgccgggcc gccatgtgtg cacgagcttg ttgcggtagg catcgaagtg
     7141 caggccgtgg ggcgccgcat ataccttgcc ccggccgagc aggatcttca acgcctgggt
     7201 accggctatg ccggcgcaga tttcgcacgc catgggtgtc gaaggtccgc gatgattgac
     7261 caggtccacc gcggacggat cgaccaggta ggactggtgg agtactgcag gcgccaatcc
     7321 caccatgaaa cgcagcaact gctcgttcat cggctgacct tcgagccgga agtactcttc
     7381 gaacgtcgtc tggcccggga ggaacgccag caccgccgca cccatgccca agggtgccgc
     7441 ggtaatcgcc gggataccgc gctcggcgca catcgcgaat accttggcgc ggatgtcgag
     7501 cacgaaaaag tccagcccgt cgacgtacag gtcgacaccc gacaggaact gctccatgtt
     7561 gtcgaaggtg acgccgttgg ggaacttgtt gatttcgaga tccggattga tgtcgcgcgc
     7621 catccgggac accacatcca ccttcggctg gccgatggtc gactggaacg cgccggcctg
     7681 ccggttcagg ttttccgtgc caaacctgtc gaggtccgcc aggttgaacg cctcgatgcc
     7741 gaggcgggtc agggtcaaca ggtgggcgcc gccgaccccg ccaagaccgg caatcgccac
     7801 gcgcttggat cggagcttcg gttgctcggc cgaggtcatc cagccgatcg tgcggctgaa
     7861 tgcggtggcg tagtcgaacg aggacggcgt gacaggggca tcggacatca tgggacacct
     7921 gcgctgcgat tgcggcttgg agcttcggcc aagccggcac cgtagcaccg gctcttggcg
     7981 agacacaacc ggcttgcggt tgaggctcca tgaggtgggg agcgcggggc gttgttcgcc
     8041 ctgttgcgcg ttcccattgc acacaaaaaa tggcgccgct tcggaaagcg gcgccattga
     8101 tggtgaaggc gctagcagtg agtcgtcgcc ttacgcgcgc cgcgaggcct ctttgcggcg
     8161 acgccggacc atgaagccca aggcacccag gccgaggccc agcaggccca gctcacccgg
     8221 ttccggtacc gacaccggca gcatcgagtt tccgattacg aaggccgttc cggaaatcgg
     8281 atagtggcag atgtcgttcg ggtcaggcga aggcgaaccg gccgtgcatc catcgatcag
     8341 cttgaccagg aaggacgaat tgatcgagaa atccgcgaat ccgccgagcc catcttgcac
     8401 cgtgttggtg tcgaaatagc ccgccactgg gccaccggtc gcatccagca cgccgatccc
     8461 gttggatcct gcctggggct cggcgatcgt gccaaggacc gtggagtaaa gctccccacc
     8521 ggcctggtcc ggcagcgtgt agcccgtgaa cgagcccgga tggccggtca ttgtcaacca
     8581 ctccgtaccg atgccggatg tggtcggatc cagcacgttg aagctcatgg tgtcgtcgac
     8641 atagaactgg atcgttccgg cgttgaacac gatctgcgtg ttcccgccca cgttgtcgat
     8701 gctctgcacc gtgtactgga aggtgaaggt caggtcgcaa cccgggcaga actcgtccgg
     8761 atccgtcccg ttgatcgagc cgatccgtcc atagcctgtg agtacgtcac cgacctggct
     8821 gatggaccct tcgcggaagt tgagcgcgtc gatgctcagg tccgctgggc tatcgggatc
     8881 gaacacgacg ccgcccacat tcacgggcgt ggcaaacgcc gctgcgctgg caccgagagc
     8941 caaggcgcca acgagagcag ctccgaggat tttcttgttc aacatgcttt tctccttcac
     9001 ttgattccat tcagttagag accaaaggca ccggcaacga gtggccttgc cctgtccgta
     9061 taaggcaaga agcatgccat gccatcaagc ctttgtatta aaagaggttt atctcctgtt
     9121 taccgtagaa ttattcgatt tctgtaaggt ctgccgacag ccttattcgg tgcctccccg
     9181 gactggatcg tgctgttgcg cctccaaggc catcctgcgg agttcgacac ggccatggtt
     9241 ccgcgcaacc cgttgcttcg ccaagagttt atgccgtcaa actacccagg agccacattt
     9301 gtcaaggttt ccgacacaga atcaagatcg ggctacagcc tcggaaaaat ccccgaaact
     9361 ggcgagcggc cgccgtccgc tgcggccggc tggaggggag cgtgcatagc cgaggcgaag
     9421 gaacaccaac ccggtgcctg tcccggtcag gtcgcccaag gccgacgcca ggcgcgaccg
     9481 cgcctgctgg aacgcgggct cgagggggat gaaaccgagg ccaagcacgc ccggtgccgc
     9541 atacggctgc gccgacaggc catcgagcgt ggcctggagc cagacgcgct gcagggcgcg
     9601 gcccccagag agcacgtcgg ctcgcgcagt ggacggcacc acgatcaggc agagccctgg
     9661 tgacagccgg atcggcaccc aggccgatcg ccagcccagc atcgatgccg cacccagccg
     9721 attgaacgag gccatgacgc cggggcgccg catggcctgg aagacggggc gaagtggcgg
     9781 ttccacggcc agcgccgccg ggggcaggcc ttcctcgcag gccgtgtgcc agccggcgga
     9841 gaagcggatg ctcgaaaaca gttccgcgtg cagtgccgga ctcttgaagc gcagcgcctc
     9901 ggcttgccgg atcaggccct gtgcggcttt tctctcccgt ggtgtctgcg gccaccaaag
     9961 ccgcgcgcca ggtatcagcc gggcctgcgc atccagcccc gctcgtgtat ccgcagtgat
    10021 cggaccgcgc cagggaaagc ggcggtcggt gtggcgctgc ccgatcgcgg ttgccagcgg
    10081 ttcgggacga tccgtagcgt cgcggcgcca acggatgcgg gcgacgcaca gggggtcgtc
    10141 ttgctgcgga aaggtttcga ggtccgcccc gtagccgcgc gaccgcgcat ggagcagcat
    10201 gttttccaga cacgcaccga tggcgaggtc gctcagcaca tagcgggtat cggatatccc
    10261 gccgctgcgg tccctgtcga tccggacgac cagatcatcg ccgctccacg caaaccgcca
    10321 cggctgcgaa ttgtctgccg acggggcggc gatgccagcc cccagaatgc ctgccttgat
    10381 gtcctggtcc acgcccttcg cctcgtccag cgtcggttgg tacggatgca cgccttcgga
    10441 tctggttggt gctgttcgtg gcccgtcggg cgcgccgcca acggctctac gttaccgctt
    10501 cgcggacgga ctgtcgaaat acactggaat ggcaatgaac ttgccccctc ggccgggcgg
    10561 ggcccacccc gaaacggacg acgaattcgc gctccgccgc cggcgacgga aagctggcgg
    10621 gacggtcggt caccgggggc atcagtcaat ttgccgccgc ggtccgcagc gcaggtggcg
    10681 cacgtcgcag aattgcgatc gaattccaaa aaagccttgg atcgagacat caatgcgagt
    10741 accctcggcc gcggttcgga aagccgcgtt ctctggttct cggtatctcc ccatgcgaag
    10801 gccgatgtct cgcctgcaga ttttgtgaac gtgttggcag aaattttccc aggatttcta
    10861 gccctttaat acgcacttca cacaatcagc tcgcataaag ttgcaaactg taacaaaggg
    10921 ggatatcgcg gctagtacgg aagctagccg tctttgagcg accgcggggg aaggatatgg
    10981 cccacggtac gcgaggccag gttgggtcgc tcagccgtga acatttccga cagctcgcac
    11041 agcgggttcc ggccaatggt cgcccaaggt gaccagccgc tgcctcagcc gcgctgcaag
    11101 tccatttcac ccattgcaac gttctcgttc cgcaccgggc tgcgccccgt gcggctgact
    11161 cggtactgca agaagcttgc caaccgcagc ctggttttgc cggtctgcga ttggcgcacg
    11221 ccgcgcgtgc ggctcactcg ataaagcagc aatagcagca caccggcggg ccgttgcggg
    11281 cccgtgccgg caaatgggga acctcgtcca tgtcgctgac ggatcggcga tgcctcgaaa
    11341 caggcgccgc ggacggcgcc gcaagcatca accaaacgga tcgtacgcca tgccccaaac
    11401 agccatcgac atcaccgcga cttccgaact ttccgcaacg cgcagcagtg aaccgacatg
    11461 ctcctgtgcg agcagccttg cagaggtgga atcagcctgg gggctggtgt accgccgcta
    11521 ttcggagatg gggttgatcg atgagaatcc gttccggatc cacgcttcgc cgaccggggt
    11581 gggcgagcat gcctgcgtga tctggggacc ggagggtccg gaaaccggtt acaccatgac
    11641 gttgttccgc gacaattcca cggggcttgc cctcgacagc gtttatcccc gggacctgga
    11701 gggaatgcgg cgtgcgggtc gcgggctgct cgaggtgggc atgctggccg atcgtcgccg
    11761 gtgcgcgtcg cgcggcatga aggcgctgtt cagcatgatg cgctgggccg tgcactacgg
    11821 attgcaatgc ggattgaccg atgtcgtgat cggcgtgcat ccgcaccacg ccccgttcta
    11881 catgcgctgt tacggcttcg agcaattcgg cgaagagacg agctacccgc tggtgcgcaa
    11941 ccatccggtt gtgggcttgc ggcttcgcct gcacgagggc ctggccaagg acgtgttgcc
    12001 ccgcggcctc gccgatgcgc gcgacaatcc ggtaccgcgc agcgcgttcg ccaatcgctt
    12061 ccggttcgag cccgcgcagc tcggcggctc ggtcatcgcc aactacatga aggcccgcta
    12121 cgggatcggg ttggaccggg tgaccggctg gacccagcct gccgctgtcg ccgccgaccg
    12181 gcc
//
LOCUS       DASSNH010000029         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_474974, whole genome shotgun sequence.
ACCESSION   DASSNH010000029 DASSNH010000000
VERSION     DASSNH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474974"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VFK00_01005"
     CDS             complement(<1..1605)
                     /locus_tag="VFK00_01005"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HET7591566.1"
                     /translation="MLAEAQSLATNVHLLGNDEYAVMLTASGAGYSHWKGRAVTRWQA
                     DQVGGEQGAYVYVRDVDGAAHWSATSQPAGGEVWYGFAQAMARFVRRDGALTTTLEVA
                     VDPDHAVEVRGVGLRNDGTVERRVDVTSYLELVLGSAPTDAAHPAFSKMFVQTAIEDG
                     VLLARRRRRDASDPGIWAAHALVVDGDTGGEREHETDRARFIGRDGSLADPAALQPGA
                     CLSGTTGTVLDPIFSLRAGLTVGAGETRRLAFVTAAAESREEVLQLIARCADARACAG
                     VFARAQRQPGPRGAGDLTPAQAALCQRLAGALVYDDPTLRPAREVVARGEGGAPVLWA
                     AGISGDLPIAVARIAQPEDMVLARDLLGAQGFWRNRQLPADLVLLDVAKGAEADAVEA
                     ALKEIAPGNDQQDKARGSVFVLRADRLDQGLRDGLMAAARIVLDGRDGGLASQSKCAA
                     VPSGVSGSPPRAAGKAPVRGGRPAAMPKPAELDFWNGLGGFTKDGREYVVVLQRGDTT
                     PVPWSHVVANAGFGFLVTATGGGYTWA"
     gene            1766..2578
                     /locus_tag="VFK00_01010"
     CDS             1766..2578
                     /locus_tag="VFK00_01010"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HET7591567.1"
                     /translation="MAETEAQPAAEAAARGAPTQATASPDPTPRLTCYRVDPRAVPIV
                     PGRRERAWMDQTADHYAYRCLPLSMANASGWELASPFAFEVTWDGNQAIEGITARAQG
                     LDPEVLRAFITSHFGHGILTFHTGWLFRTSPGWGLWVRGAPNYAKDGIHPLDGMVETD
                     WLPFPFTMNWRFTRPCTVRFGTGDPFCFITLCPHALLDDITPTRADIEDDPGLKADYA
                     EWAASRAEFNKLLHDGDPATVARKWQRGYFQGKGTGGEAPFHVSKRRLKPLK"
     gene            2697..>2957
                     /locus_tag="VFK00_01015"
     CDS             2697..>2957
                     /locus_tag="VFK00_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7591568.1"
                     /translation="MQLGMIGLGKMGNFMAQRLMKGGHDVVGFDPSDKARTALADAGG
                     KAVDTLEALVRALEPPRAVWVMVPAGKIVDETVAGLAAVLGEG"
BASE COUNT          460 a         1081 c         1006 g          410 t
ORIGIN      
        1 cgcccacgtg tagccgccgc cggtcgcggt cacgaggaag ccgaagccgg cgttggcgac
       61 gacgtgcgac cagggcaccg gcgtggtgtc gccacgctgg agaaccacca cgtactcacg
      121 gccgtctttc gtgaaaccgc cgaggccgtt ccagaaatcg agttcggcag gcttcggcat
      181 cgcggcgggg cggccgccgc gcaccggcgc ttttcccgcc gccctcggcg gtgagccgct
      241 gacgccggag ggcacggcgg cacatttcga ttggctggcg agcccgccgt cgcggccgtc
      301 gaggacgatc ctcgccgccg ccatgagtcc gtcgcgcaat ccctggtcga gccggtccgc
      361 gcgcagcacg aacaccgaac cgcgcgcctt gtcctgttgg tcgttgccgg gtgcgatctc
      421 cttgagcgcg gcttcgaccg cgtcggcctc cgcgcccttg gcgacgtcga gaagcaccag
      481 gtcggcgggc aactggcgat tgcgccagaa gccctgcgcg cccagcaggt cccttgccag
      541 caccatgtct tcaggttggg cgatccgggc aaccgcgatc ggaaggtcgc cggaaatacc
      601 cgccgcccac aacaccggcg cgccgccctc gcccctggcg accacctcgc gggctggacg
      661 caatgtcgga tcgtcgtaga caagcgcacc tgccagccgc tggcacagcg cggcctgggc
      721 gggggtgagg tcgccggcgc cgcgcggacc cggttgccgc tgcgcgcgcg cgaacacccc
      781 agcgcacgcc cgggcgtccg cgcaacgcgc gatcagttgc aacacctcct cgcgcgattc
      841 cgcggccgcg gtgacgaacg ccagccgccg cgtctcgccg gcgcccacgg tcaagcccgc
      901 gcggaggctg aagatgggat cgaggacggt gccggtggtc cccgacaagc atgcgccggg
      961 ttgcagcgcc gcgggatcgg ccagcgagcc gtcacggccg atgaagcggg cgcggtcggt
     1021 ttcgtgttcg cgctcgccgc cggtgtcccc gtcgacgacc agggcgtggg cggcccagat
     1081 gccgggatcg gaggcgtcgc gcctgcgccg ccgcgccaac agtacgccgt cctcgatcgc
     1141 ggtctgcacg aacatcttcg aaaacgccgg gtgcgctgcg tcggtgggcg cggaacccag
     1201 gaccagttcc aggtaagacg tcacgtcgac ccggcgctcg accgttccgt cgttgcgcag
     1261 gccgacgccg cggacttcca ccgcgtggtc cggatcgacc gcgacttcga gcgtggtggt
     1321 cagcgcacca tcgcgccgga cgaagcgcgc catcgcctgg gcgaacccgt accacacctc
     1381 gccaccggcc ggctgcgacg tcgccgacca atgcgcggcc ccgtcgacgt cgcggacata
     1441 cacgtacgcg ccctgctcgc cgccgacctg atcggcttgc cagcgggtga ccgcacggcc
     1501 cttccaatgg ctgtagcccg cgccggacgc ggtcagcatc accgcatatt cgtcattgcc
     1561 gagcaggtgg acattcgttg cgagcgattg ggcttcggca agcatggtcg agggctcccg
     1621 ctcccttcgc atcaacacgt gattatcacg ccgcgcacat caatcgttcg tgaaacgcga
     1681 accttgaccc ctcccgcgaa acgccggacg atggagcgcc cggcatcgcc gccacgggcg
     1741 aagccgcggt cacggaggag cgtcgatggc cgaaaccgaa gcgcaacctg ccgcggaggc
     1801 ggcggcgcgg ggcgcaccca cgcaggcgac cgcatcgccc gatcccacgc cgcggctgac
     1861 atgctatcgc gtcgatccgc gcgcggtgcc gatcgtcccc ggacgccgcg agcgcgcctg
     1921 gatggaccag accgccgacc actacgcgta ccgctgcctg ccattgtcga tggccaatgc
     1981 cagcggctgg gagctggcgt cgccgttcgc cttcgaagtc acctgggacg gcaaccaagc
     2041 catcgagggc atcacggcgc gtgcgcaggg cctcgacccc gaagtcttgc gcgcattcat
     2101 cacctcgcat ttcggccacg gcatcctcac cttccacacc ggctggctgt tccgcacctc
     2161 gcccggctgg ggcctttggg tgcgcggcgc gccgaactac gcgaaggacg gcatccaccc
     2221 actggacggc atggtcgaga ccgactggct gccgttcccg ttcacgatga actggcgctt
     2281 cacccggccc tgcacggtgc gtttcgggac cggcgatccg ttctgcttca tcaccctgtg
     2341 cccccacgcg ctgctggacg acatcacgcc gacgcgcgcc gacatcgagg acgaccccgg
     2401 cctcaaggcc gactacgcgg aatgggccgc gtcgcgcgcc gagttcaaca agttgctgca
     2461 cgatggcgat cccgcgaccg tcgcgcgcaa gtggcagcgt ggctacttcc agggcaaggg
     2521 cactggcggc gaagcgccgt tccacgtcag caagcggcgg ctgaagccgc tcaagtgatc
     2581 cggcagaagc gcaccgcggc tggcacgcgc gcggaccgcc cgcctgcgcg ggcgtaccat
     2641 ggcggattgc gcgggccgcg cggcgcgcca tcaccacgaa gccactggag gacaacatgc
     2701 aactcggaat gatcggcctg ggcaagatgg gcaacttcat ggcccagcgc ctgatgaaag
     2761 gtggccacga cgtggtcggc ttcgatccga gcgacaaggc acgcacggcg ctggctgatg
     2821 ccggcggcaa ggcggtcgat acgctcgagg cgctggtccg cgcgctcgaa ccgccccgcg
     2881 ctgtatgggt gatggtgccg gccggcaaga tcgtggacga aaccgtcgcg ggtctggcgg
     2941 ccgtgctcgg cgaaggc
//
LOCUS       DASSNH010000030        13220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_601131, whole genome shotgun sequence.
ACCESSION   DASSNH010000030 DASSNH010000000
VERSION     DASSNH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601131"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFK00_01020"
     CDS             complement(<1..239)
                     /locus_tag="VFK00_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7591569.1"
                     /translation="MTTLQELASQHCEPRKGGRALDSARVGELLASLPGWAAAGDGKS
                     IAKSFKFANFYETLGFVNAIGWMANKQDHHPDLEVG"
     gene            complement(250..609)
                     /locus_tag="VFK00_01025"
     CDS             complement(250..609)
                     /locus_tag="VFK00_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7591570.1"
                     /translation="MQPRTTLACLVAASLMLAFGSAAAASGNAAEGAKKAAACEACHG
                     KDGNGIAPNYPALAGQYQDYLEQALHEYKNGRRTNAIMNGMAAPLSDQDIEDLAAHFS
                     SLPSRLSDLHGKIEGGN"
     gene            complement(622..963)
                     /locus_tag="VFK00_01030"
     CDS             complement(622..963)
                     /locus_tag="VFK00_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7591571.1"
                     /translation="MIRSVLTVVLVAAFACFASTAFAKGDPAKGKLEVYTCHGCHGVT
                     GYMSVYPEYHVPRIAGQNEQYIIDALKEYKSGARKFPTMNAQASSLTDEQIENIAAYL
                     SSLAPKQPHKN"
     gene            complement(1070..1417)
                     /locus_tag="VFK00_01035"
     CDS             complement(1070..1417)
                     /locus_tag="VFK00_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7591572.1"
                     /translation="MDHPMFPQSNADIAKKRHELAPGPLEAFQNFSRAVFADGALPSV
                     TKQLIAVAVAHTTQCPYCIKGHTRAALRAGATEQQIMEAIWVAAEMRAGGAYAHSALA
                     IDVMQQHEASHAG"
     gene            1561..2928
                     /locus_tag="VFK00_01040"
     CDS             1561..2928
                     /locus_tag="VFK00_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET7591573.1"
                     /translation="MKTWRFGVWGLFALLGLATAWAAAPQSPAARPVVVTLRVDGAIS
                     PASADYLVRGIAEADQRHAEAIVLELDTPGGLSSSMRDIIKAILASPVPVIGYVSPSG
                     ARAASAGTYIMYACQVAAMAPATNIGAASPVSITGGGSLPVPSSSSGKPADSESTEMR
                     KVTNDAVAYIRALAERRGRNADWAEKAVREAVSVSDSEALKLRVIDLVAPNLTQLMAD
                     VDGRKVETAAGARVLHTRGAELQAIEPGWRSQFLGVVANPSVAYILLLIGLGGLVMEG
                     MHPGGVLPGVVGAICLLLALYAFQLLPVNYVGLGLILLGVILIVSEAFVPAYGVLGTG
                     GVISFVIGSVIFMHTGVPGYGIAVPVVVGIAIAAAAILVGIVWMAVRSHKQPVVSGRE
                     EMVGAIGEVVADFVGRGAVHVHGERWQARSDVPLRRGQPVAVTAMHGLILDVKPLAVP
                     KEATP"
     gene            2931..3707
                     /locus_tag="VFK00_01045"
     CDS             2931..3707
                     /locus_tag="VFK00_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET7591574.1"
                     /translation="MYPVIAVIVVIVLIILLSSIKVLREYDRGVVFLFGRFQAVKGPG
                     IIILIPIMQQMVRIDLRTIVVEVPPQDVISRDNVPVKVNAMILYRVVDPEKAVIQVED
                     AREATSQLAQTTLRSVLGRHELDEMLSEREKLNADIQGILDQHTDAWGIKVSNVEIKQ
                     VDLEESMYRAISRQAEAERQRRAKIINAEGELQASEKLMQAAQTLATQPQSIQLRYLQ
                     TLVEVAGDKTTTIAFPLPLDLLEPLLGKLRKDSGTTTPAP"
     gene            complement(3954..4877)
                     /locus_tag="VFK00_01050"
     CDS             complement(3954..4877)
                     /locus_tag="VFK00_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET7591575.1"
                     /translation="MSTPKTIKEYLAQLRAALEGADPAMIQDALYDAEEHLRSELAEN
                     PGMGEAELLAKIATSYGAPEEVAEIYRTTEQTVARALRRPPPPPRRSAIGRFFGVLAD
                     PHTYGALFYMLLALATGIFYFTWAVVGLSLSTGLAFTLIGIPFFLLFMASVRGLSLLE
                     SRIVEGMLGVRMPRRPPYVERDRPWLKRIGSMLADARTWFTLFYMLLMLPLGIAYFVI
                     VAVLSAVSLALMLTPIAMAFDFFGVGRDFVVDHVTINWGFGVHIPGWGDAAVMFVVGF
                     FLLFGMLHLVRAIGHLHGALAKHLLVKSARV"
     gene            complement(4882..5238)
                     /locus_tag="VFK00_01055"
     CDS             complement(4882..5238)
                     /locus_tag="VFK00_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7591576.1"
                     /translation="MSDTEAHLKKFEKELAAGTVSLVLLAALGAAREPMYGYQIAKRL
                     ERDAGGVLVGKQSALYPVLRNLSAAGLLDTFVEPSEAGPPRRYYRITSLGRRVLREWA
                     DAWDSTRRYVDSILKD"
     gene            5380..5712
                     /locus_tag="VFK00_01060"
     CDS             5380..5712
                     /locus_tag="VFK00_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591577.1"
                     /translation="MDSIATPRDGEEVRMFEIAVVLLAVFGAVWLFGALVVGAFKLTF
                     GLLGVIFGGLFGLFALAVTALVVIPVVLFAMLPVLLPALCVVGLVWLIVHASRPHAGP
                     PQGNARPG"
     gene            complement(5727..6386)
                     /locus_tag="VFK00_01065"
     CDS             complement(5727..6386)
                     /locus_tag="VFK00_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7591578.1"
                     /translation="MTSLRSDPSFECAPHAARSIALALALSLNLTLVLFALRPHTPFE
                     LHAPPSPSLLATLLQPPPPPAPAPAVPILRAVQHVAIPTVPVAVTHPLPLATPALPMI
                     GPVGSIRTVPATSVEVAPAGTAGDADATIAYETATAPSYPIAALRAGIQGTVLLKVLV
                     DPRGQPIRVVVEHGSGSRILDDAARRHVLTAWRFHPAVRNGHAIEAWALVPVHFKLDR
                     G"
     gene            complement(6495..7079)
                     /locus_tag="VFK00_01070"
     CDS             complement(6495..7079)
                     /locus_tag="VFK00_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7591579.1"
                     /translation="MRAQPTSEEASWPETLWTIGHSTRGIDEFIALLQQNDIEVLADV
                     RHFPGSRKYPHFNAEPLREAMHGVGIDYEPFIELGGRRKVQPDSPNTAWRHPAFRGYA
                     DYMQTDAFRTGIERLKALASAKRTAIMCSEAVWWRCHRGLVSDVFKLNGTRVLHIMGP
                     GEPREHPYTSAAHVADGRLDYAADQHELPIRGEP"
     gene            7187..7957
                     /locus_tag="VFK00_01075"
     CDS             7187..7957
                     /locus_tag="VFK00_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7591580.1"
                     /translation="MIGYATANTVEEFRQHLADVHARIGAACTKAGRDAAGVRLLPVS
                     KTVSEERIRNAYAAGCRTLGENKVQEAHRKWQALSDLPDLRWAVIGHLQTNKARQVAE
                     FASEFQALDSLRVAEALDKCLQSLGRSLDVLVQVNTSGEESKFGLPPDGVAAFVRRLP
                     AFSSLRVRGLMTLALFSDDAAKVRPCFVCLRELRDRLRQGAPEGVELDELSMGMSGDY
                     ELAIEEGSTIVRVGQAIFGARKTPDAAYWPGGGAPEAR"
     gene            complement(7960..8439)
                     /locus_tag="VFK00_01080"
     CDS             complement(7960..8439)
                     /locus_tag="VFK00_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7591581.1"
                     /translation="MASRIALRQAATPQQVATVRELFREYAQAIGTDLEFQGFSGELA
                     ALPKPYVPPKGALLLAQAGGAVAGCVGLRPLEGGLGEMKRLYVRPAYRKFGLGRRLVQ
                     AVVDAARAAGHRELRLDTLASMGPAQALYRQLGFVEIAPYNPLHMPGTRFYSLDLSS"
     gene            complement(8469..8807)
                     /locus_tag="VFK00_01085"
     CDS             complement(8469..8807)
                     /locus_tag="VFK00_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET7591582.1"
                     /translation="MATDLSFIEYVADQVRLQDRLTHRKMFGEYALYLDDKVVAFACD
                     NSLFVKPAEGTRHLTADLPQQPPYPGAKLYPVADALLDEPDLLRGLLLATERALPAPR
                     PKAPRKRSTK"
     gene            complement(8827..10017)
                     /locus_tag="VFK00_01090"
     CDS             complement(8827..10017)
                     /locus_tag="VFK00_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET7591583.1"
                     /translation="MQDQPVKRVTRDGVEFVLLGTAHVSRASVDAVKALVEREPFDAI
                     AIELCESRARGIRDPEALANMDLFQVIRTGKAGMVFASLALASFQQRIAEQYGIEPGA
                     EMKAAMAAADARGLPLWLVDREIGITLNHAYRGVPFRDRMGILGGLIASVFSREEIDE
                     ADIEKLKQGDILESAFGEFARESKPLYEALIGERDRFMAARLREESATNPVARRVLLV
                     IGAGHLKGVERELGAQQQPPRAIIEPLAAKLPAPKWPKYFAICVMLAIFAAIGFLFYR
                     NATMGWHALRDWVIYTGLLSALGAAIAGGHPLSVLTAAVMGPLKPLRPPGLSSGVFSG
                     MAEAWLRKPRVADFQSLRHDLLHVTGWWRNRVARTLLVFMLTNLGTIAGEYLAAARIF
                     TRLL"
     gene            complement(10065..10952)
                     /locus_tag="VFK00_01095"
     CDS             complement(10065..10952)
                     /locus_tag="VFK00_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HET7591584.1"
                     /translation="MTNKTLKLALLQETDRGSTDANLDAIEAGLREAARAGAQLVLLQ
                     EIHNGPYFCQHESVAEFDRAETIPGPSTERIGKLAAELELVVVASLFERRAAGLYHNT
                     AVVFDRSVGIAGTYRKMHIPDDPAYYEKFYFTPGDLGFHPIATSAGKLGVLVCWDQWY
                     PEAARLMALAGAEILLYPTAIGWDPADAQDEKDRQRDAWITVQRGHAVANGVPLAACN
                     RVGFEPSPDGGRGAQFWGSSFVAGPQGEILAQAGSDKRELLIADVDLERSESVRRIWP
                     FLRDRRIDAYGDLTRRFRD"
     gene            complement(11006..12049)
                     /locus_tag="VFK00_01100"
     CDS             complement(11006..12049)
                     /locus_tag="VFK00_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET7591585.1"
                     /translation="MPSPGLRLPAEWEPQSAILLAWPNAGTDWAARLDEVEESYAALV
                     AAITRFEPVIVCVPDGEIGAHAAALLGDAGADLGQVRFVEIPYDDTWLRDSGPITLRD
                     AAGGFVLVDFRFTGWGGKFEASQDDALVAGLVERGVFRSGARHQRVDWALEGGAIESD
                     GRGTILTTWRCLQTRHPDEPRGQIELTLRNALSASRVLWLEHGFLQGDDTDAHIDTLA
                     RLAPDDAIVYQACSDADDPHYRELDMMRRDLEALRTLGGDPYRLFPLPWPKPIVEEGR
                     RLAASYANYLVINGAVLVPAYGDIADGAAAETIRLAHPGREVVQIPCRPLIWQNGSLH
                     CVTMQLPEGVMFL"
     gene            complement(12284..12628)
                     /locus_tag="VFK00_01105"
     CDS             complement(12284..12628)
                     /locus_tag="VFK00_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591586.1"
                     /translation="MNRIATSSTAVIFALAAGVLALPAQADTLQTQTQSVPLSAKVQQ
                     EQHMDLPTRGMTMDQVKREYGAPLKVLATRGGSSKYQPPIHRWEYSKYIVYFEYKHVI
                     HSVLRTPGPDSL"
     gene            complement(12866..12991)
                     /gene="ykgO"
                     /locus_tag="VFK00_01110"
     CDS             complement(12866..12991)
                     /gene="ykgO"
                     /locus_tag="VFK00_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HET7591587.1"
                     /translation="MKVLSSLKSAKQRHRDCKIVRRRGKVFVICKSNPRFKARQR"
BASE COUNT         2122 a         4248 c         4594 g         2256 t
ORIGIN      
        1 ccgacctcga ggtcggggtg gtggtcctgc ttgtttgcca tccagccgat cgcgttgacg
       61 aagccgagcg tctcgtagaa gttggcgaac ttgaaggatt tcgcgatcga cttgccgtcg
      121 cccgccgcgg cccaacccgg cagcgaagcc agcaattcac cgacccgtgc cgagtccagc
      181 gcgcgaccgc ccttgcgcgg ttcgcaatgc tgtgaggcca gttcctgcag ggtcgtcatg
      241 tccgttacct cagttgccgc cctcgatctt gccgtgcagg tcggaaagcc tgctcggaag
      301 gctggagaaa tgcgcggcga gatcctcgat gtcctggtcg gacagcggcg cggccatgcc
      361 gttcatgatc gcgttggtgc gccggccatt cttgtattcg tgcagcgcct gttccaggta
      421 atcctggtac tggccggcga gggcggggta gttcggcgcg atgccgttgc cgtccttgcc
      481 gtggcaggcc tcgcaggccg ccgctttctt ggcgccttcg gcggcgttgc cgctggctgc
      541 ggcagcggag ccgaatgcaa gcatcagcga ggcggcgacc aggcaggcga gggtggtacg
      601 aggttgcatg gatcggtccc cttagttctt gtgcggttgc ttgggcgcga ggctggacag
      661 gtaagccgcg atgttctcga tctgctcgtc ggtcaggctg ctggcctgcg cgttcatggt
      721 cgggaacttg cgggcgccgc tcttgtattc cttcaacgcg tcgatgatgt actgctcgtt
      781 ctgaccggcg atgcgcggca cgtggtattc ggggtacacc gacatgtagc cggtgacgcc
      841 gtggcagcca tggcaggtat agacctcgag cttgcccttg gccgggtcgc ccttggcgaa
      901 agccgtggac gcgaagcacg cgaacgccgc caccaggacg acagtcagca ccgatcgaat
      961 catgttgttg ggctcccccg ctggcggcgt tggccgtggt tgcaaagccc gaagtatagc
     1021 ctgtgatgcc gcagggggga caagggtgcg cgcggaaagc ggcccgcgat cagcccgcat
     1081 gcgacgcttc gtgctgctgc atcacgtcga tcgctagcgc cgaatgcgca tacgctccgc
     1141 ccgcgcgcat ctcggcggcg acccagatcg cttccatgat ttgctgctcg gtggcgccgg
     1201 ccctgagggc ggcgcgcgtg tggcccttga tgcagtacgg acactgcgtg gtatgcgcca
     1261 ccgccaccgc gatcaactgc ttggtgaccg agggcagggc gccgtcggcg aacacggcgc
     1321 ggctgaagtt ctggaaggcc tccagcggcc ccggggcgag ttcgtggcgc ttttttgcga
     1381 tgtcggcatt cgattgcgga aacattggat ggtccatggc gttctctcgt cttggtcgat
     1441 ttgaaccgga cactgtacgc cgaccgcccg cggctgcctt gccgcatgtc aacgatcgca
     1501 tccgccggcg ccttgaccat ggggtttgcg aagttcggcg ggtaatggtc tgatggcgcc
     1561 atgaagacgt ggcggttcgg ggtctgggga ctgtttgcgt tgctggggtt ggcgacggcg
     1621 tgggctgccg cgccgcaatc gccggcggcc cgtcccgtgg tcgtgacgtt gcgcgtcgat
     1681 ggcgcgatca gccctgcgag cgctgattac ctcgtgcgcg gcattgcgga ggcggaccag
     1741 cggcacgccg aggcaatcgt gctggagctc gacaccccgg gtggactctc gagttcgatg
     1801 cgcgacatca tcaaggcgat actcgcctcg ccggtgccgg tgatcggcta tgtctcgccg
     1861 tccggcgcgc gcgcggccag cgcgggaacc tacatcatgt acgcctgcca ggtcgcggca
     1921 atggcgcccg cgaccaacat cggcgccgcg tcccccgtgt cgatcaccgg cggcggcagc
     1981 ctgccggtgc cgtcctccag cagcggcaag ccagcagatt ccgaatcgac ggaaatgcgc
     2041 aaggtcacca atgacgcggt ggcctacatc cgcgcgctcg ccgagcggcg cggccgcaat
     2101 gcggattggg cggagaaggc cgtgcgcgag gcggtcagcg tttcggacag cgaggcgctg
     2161 aagttgcgcg tgatcgacct ggtcgcaccg aacctcacgc aactcatggc cgacgtggac
     2221 ggccgcaaag tggaaaccgc tgccggcgcg cgggtattgc acacgcgtgg cgccgagttg
     2281 caggcgatcg aaccgggatg gcgcagccag ttcctcggcg tggtcgccaa tccctcggtc
     2341 gcgtacatcc tgttgctgat cggcctcggg ggcctcgtga tggagggcat gcatcccggc
     2401 ggggtgttgc cgggcgtggt cggcgcgatc tgcctgctgc tcgcgctgta cgcgttccag
     2461 ttgctgccgg tgaactacgt gggactcggc ctgatcctgc tcggggtgat cctgatcgtc
     2521 tcggaggcgt tcgtgcctgc gtacggggtg ctcggcaccg gtggagtgat ttcgttcgtg
     2581 atcggttcgg tgatcttcat gcacacaggc gtaccgggct acggcatcgc ggtgccggtg
     2641 gtggtcggca tcgcgatcgc ggccgccgcg atcctcgtcg gcatcgtgtg gatggcggtg
     2701 cgctcgcaca agcagccggt ggtcagcgga cgcgaggaaa tggtcggcgc gatcggcgag
     2761 gtggtcgccg acttcgtggg ccgtggcgcg gtgcacgtgc acggcgaacg ctggcaggcg
     2821 cgttccgacg ttccgctcag gcgcggccag ccggtcgccg tcaccgccat gcatggcctc
     2881 attctcgatg tgaaaccctt ggctgttccg aaggaggcaa cgccgtgagc atgtatcccg
     2941 tcatcgcagt gatcgtcgtc atcgtgctga tcatcctgct gtcgtcgatc aaggtcctgc
     3001 gcgagtacga ccgtggcgtc gtgttcctgt tcgggcgctt ccaggcggtg aaggggccgg
     3061 gcatcatcat cctgatcccg atcatgcagc agatggtccg gatcgacctg cgcacgatcg
     3121 tggtggaggt gccgccgcag gacgtgatct cgcgcgacaa cgtgccggta aaggtcaacg
     3181 cgatgatcct ctaccgcgtg gtcgacccgg aaaaggcggt gatccaggtg gaagacgcgc
     3241 gcgaggccac cagccagctt gcccagacga cgttgcgctc ggtgctggga cggcacgaac
     3301 tcgacgaaat gctgagcgag cgcgaaaagc tcaatgccga catccagggc atcctcgacc
     3361 agcacaccga cgcgtggggc atcaaggtct ccaacgtcga gatcaagcag gtggatctcg
     3421 aggaaagcat gtaccgcgcg atttcgcgcc aggccgaggc cgagcgccaa cggcgcgcca
     3481 agatcatcaa cgccgagggc gaactgcagg cgtccgagaa gctgatgcag gccgcgcaga
     3541 ccctcgccac gcagccgcaa tcgatccagt tgcgctactt gcagacgctg gtcgaagtcg
     3601 cgggcgacaa gaccacgacc atcgcgttcc cgctgccgct ggatctgctg gagccgttgc
     3661 tcggcaagtt gcgcaaggat tccgggacga cgacgccggc gccgtgatgc ggtagtgcgg
     3721 atgaaacgga gtcgaaaccg gggggggcgg gctcattccc gcattggttc gcgacatcca
     3781 ggtcgcccac gctccgggcc ggcttggccg ttcttgtttg ctgtcctgca aacgcggtcg
     3841 gcgctgcgcg acgcatgcgg cttggctgcc tgctggcacg gcaacgcgtg aacctgacga
     3901 ccgacggacg atgcccgtct tgcggcgcga gcctctgccg atcccgggcg gcttcaaacc
     3961 cgcgcggatt tcaccagcag gtgcttggcc agcgcgccgt gcaggtgccc gatcgcgcgc
     4021 acgaggtgca gcatcccgaa cagcaggaag aagccgacca cgaacatcac cgccgcgtcg
     4081 ccccagcccg ggatgtggac tccgaagccc cagttgatcg tcacgtggtc gacgacgaaa
     4141 tcgcgtccca cgccgaagaa gtcgaacgcc atcgcgatcg gcgtgagcat cagcgccagc
     4201 gaaacggccg acagcaccgc cacgatcacg aagtacgcga tgcccagcgg cagcatcagc
     4261 aacatgtaga acaaggtgaa ccaggtgcgc gcatccgcca gcatgcttcc gatgcgcttg
     4321 agccagggcc gatcgcgttc gacgtacgga gggcgacgcg gcatccgcac cccgagcatg
     4381 ccctcgacga tgcggctttc cagcagcgac aggccccgaa ccgacgccat gaacagcagg
     4441 aagaacggaa tgccgatcag cgtgaaggcg aggccggttg aaagggatag cccgacaacc
     4501 gcccaggtga agtagaagat gccggtggcg agcgccagca gcatgtagaa cagcgcgcca
     4561 tacgtgtgcg ggtcggcgag cacgccgaag aagcggccga tggcggagcg gcgcggcggc
     4621 ggcgggggcc gacgcaacgc gcgtgccacc gtctgctcgg tggtgcggta gatctcggcg
     4681 acttcctcgg gcgcgccgta gctggtcgcg atcttcgcca gcagctcggc ctcgcccatg
     4741 cccgggttct cggcgagttc cgagcgcagg tgctcctcgg cgtcgtacag cgcgtcctgg
     4801 atcatcgccg gatcggcacc ctccagtgcg gcacgcagtt gcgcgaggta ttccttgatg
     4861 gtcttgggcg tactcatggc ttcaatcctt caagatcgag tcgacgtaac ggcgggtgga
     4921 atcccaggca tcggcccatt cgcgcaggac ccgccggccc agcgacgtga ttcggtagta
     4981 gcggcgcggc ggcccggctt cggagggttc gacgaaggtg tcgagcaacc cggcggcgct
     5041 caggttccgc aacaccgggt acagcgcgct ctgcttgccc accagcacgc cgccggcatc
     5101 gcgctcaagg cgcttggcga tctggtagcc gtacatcggc tcgcgagcgg cgcccaatgc
     5161 ggccagcagg accagcgata ccgtgccggc agccagttcc ttctcgaact tcttgaggtg
     5221 cgcttcggtg tcgctcatcg tgagcttgca tcgcccgtac agacgagtac actataccgc
     5281 acttgatagt agttgcgtca gccagaagtc atgggcatgt cgatgggtcg cgagtcgtgc
     5341 ttctgcacgg tgcggcgaag gcgcatactg gatgcgcgca tggattcgat tgcgacgccg
     5401 cgggacggcg aggaggtcag gatgttcgaa atcgcagtgg tactgctggc cgtgttcggt
     5461 gcggtctggc tgttcggcgc cctcgtcgtc ggcgcgttca agctgacgtt cggactgctg
     5521 ggcgtgattt tcggcggcct gttcgggctg ttcgcgctcg ccgtcaccgc cttggtcgtg
     5581 attccggtcg tcctgttcgc gatgctgccg gtgctgctcc ccgcgctctg cgtcgtcggc
     5641 ctggtgtggc tgatcgtgca cgcatcgcgc ccacacgccg gcccgccgca gggtaacgcc
     5701 cgccccgggt agacggcgcg gaggcgttac ccgcggtcca gcttgaagtg cacgggcacc
     5761 agcgcccacg cttcgatcgc gtggccgttc cgcacggccg gatggaaccg ccacgccgtg
     5821 agtacgtgcc tgcgtgccgc atcatccagt atccgcgagc cgctgccgtg ttcgaccacg
     5881 acccggatag gctgtccgcg cgggtcgacc aacaccttga gcagcaccgt gccctggatg
     5941 ccggcgcgca gcgccgcgat cgggtacgag ggtgccgtgg cggtttcgta ggcgatggtc
     6001 gcgtcggcat cgcccgcggt gccggccggg gccacttcca ctgacgttgc gggaaccgtc
     6061 cggatggatc ccacgggccc gatcatcgga agcgccggcg tggcaagggg cagcgggtgc
     6121 gtcaccgcca cgggtacggt cgggatggcc acgtgctgta ccgcgcgcag gatcggcacg
     6181 gccggcgccg gcgccggcgg tggcggcggc tgcagcagtg tggcgagcag cgacggcgag
     6241 ggtggtgcgt ggagctcgaa cggcgtatgc ggccgcagcg cgaacaacac gagggtcagg
     6301 ttcaagctga gggccagcgc cagcgcgatg gagcgggcag cgtgcggcgc gcactcgaag
     6361 gaaggatcgg agcgcaatga cgtcatgagc cacctccttg cgtgaagaac gtggccaggg
     6421 acgtccggga acgacgcccc ggtcgagctt cacgctacgc cttcagtcga tcccggacaa
     6481 gtgccggcgg gtgctcacgg ctcgccgcgg atcggcagtt cgtgctggtc cgccgcgtag
     6541 tcgagccttc cgtccgccac gtgtgccgcc gacgtgtacg ggtgttcacg tggttcgccg
     6601 ggtcccatga tgtgcagcac gcgcgtgccg ttgagcttga acacgtcgga aaccaacccg
     6661 cggtggcaac gccaccacac cgcttccgag cacatgatcg cggtgcgctt cgcgctggcc
     6721 agtgccttca gtcgctcgat gcccgtgcgg aaggcgtccg tctgcatgta gtccgcgtag
     6781 ccgcggaacg ccggatgccg ccaggccgtg ttcggcgaat ccggctgcac cttgcggcgg
     6841 ccgcccaact cgatgaaagg ttcgtaatcg atcccgacgc cgtgcatcgc ctcgcgcagc
     6901 ggctcagcat tgaagtgcgg gtatttgcgc gagccgggaa agtggcgaac gtcggccagc
     6961 acctcgatgt cgttctgctg caacagcgcg atgaattcgt cgatgccacg cgtcgaatgc
     7021 ccgatggtcc acagtgtttc gggccatgac gcttcttcgg atgtcggctg cgcgcgcatg
     7081 cgaacaggat acgcgttcca tcgcagccgt tcgccacggg tgcggcgtcg taggcgggga
     7141 gccgaatggt gggaggtcac gcgcacgtgc tgtctatact ggcgggatga ttggttacgc
     7201 caccgccaat accgtcgagg aattccgcca gcacctcgcc gacgtgcatg cgcgcatcgg
     7261 cgcggcctgc acgaaggccg ggcgagatgc tgcgggcgtg cgcctgttgc cggtcagcaa
     7321 gaccgtttcc gaagaacgca tccgcaacgc ctacgccgca ggttgccgca cgttgggcga
     7381 aaacaaggtg caggaggcgc atcgcaagtg gcaggcgctt tcggacctgc cggatctccg
     7441 ctgggccgtc atcggccacc tgcagaccaa caaggccagg caggtcgccg agttcgcgtc
     7501 agagttccag gcgctggaca gcctgcgcgt cgccgaggcg ttggacaaat gcttgcagtc
     7561 gctcggccgg tcgctcgacg tgctggtgca ggtcaatacg tccggcgagg aaagcaagtt
     7621 cggcctgccg ccggatggcg tggcggcatt tgtccggcgg cttccggcct tctcttcgct
     7681 gcgcgtgcgc ggactgatga cactggcgct gttctcggac gacgcggcga aggtgaggcc
     7741 gtgcttcgtg tgtctgcgcg aattgcgcga ccggctgcgc cagggggcgc ccgagggcgt
     7801 cgaactggac gagctctcga tgggcatgtc gggcgattac gaactcgcga tcgaagaagg
     7861 ctcgaccatc gtgcgcgtgg ggcaggcgat cttcggtgcg cgcaagacgc cggacgccgc
     7921 ttactggccc ggcggcggtg cgccggaagc ccggtgaagt cacgacgaca ggtcgagtga
     7981 atagaaccgc gtgccgggca tgtgcaacgg gttgtaaggc gcgatttcga cgaacccgag
     8041 ctggcgataa agcgcctgcg ccgggcccat gctcgccaac gtgtcgaggc ggagttcgcg
     8101 atgaccggct gcgcgcgcgg cgtcgaccac ggcctgcacg aggcgccggc ccaggccgaa
     8161 cttcctgtag gcgggcctga catacaggcg cttcatttcg cccaggccgc cttcgagcgg
     8221 tcgcagtcct acgcagccgg cgacggcgcc acccgcttgc gcgagcagca aggcgccctt
     8281 gggaggtacg tacggcttcg gcagtgctgc aagttcgccg gagaagcctt ggaactcgag
     8341 atccgtgcct atggcctggg catattcgcg gaaaagttcg cgaacggttg cgacctgctg
     8401 cggcgtggcg gcctggcgca atgcgatgcg ggatgccacc gtcgcatcgc gaaccgatgg
     8461 cgggtgcatc actttgtgga ccgcttccgc ggtgccttgg gcctaggggc cggcagagcg
     8521 cgttcggttg caagcagcaa cccacgcagc aggtccggct cgtccagcag tgcgtccgca
     8581 accgggtaca gcttggcccc tggatacggc ggttgctgcg gcaggtcggc cgtcagatga
     8641 cgggtgccct cggccggctt gacgaacagg ctgttgtcgc aggcgaacgc aaccaccttg
     8701 tcgtcgaggt aaagcgcgta ctcgccgaac atcttcctgt gcgtcaggcg atcctgcagc
     8761 cgcacctggt ctgcgacgta ctcgatgaag gacaagtcgg ttgccatggt cgcccccgct
     8821 ggcggcttac aacaaccggg tgaagatgcg cgcggcggcg aggtattcgc cggcgatggt
     8881 gccgaggttg gtgagcatga acaccagcag ggtccgtgcg acgcggttgc gccaccagcc
     8941 cgtcacgtgc agcaggtcgt ggcgcagtga ctggaagtcc gccacacggg gcttgcgcaa
     9001 ccacgcttcg gccatgccgg agaagacgcc ggacgacagt ccaggcgggc gcaatggttt
     9061 cagcgggccc atcaccgcgg cggtgagcac cgacagggga tgcccgcccg cgatcgcggc
     9121 gcccagcgcg ctcaacagcc cggtgtagat cacccagtcg cgcagcgcgt gccagcccat
     9181 ggtcgcattg cgatagaaca gaaatccgat cgcggcgaag atcgccagca tcacgcagat
     9241 ggcgaaatac ttcggccact tcggagccgg cagcttggcg gcaagcggtt cgatgatcgc
     9301 ccgcggcggt tgctgctgcg cgccgagttc acgttccacg cccttgaggt ggcccgcgcc
     9361 gatcacgagc agcacccgcc tggcgaccgg gttcgtggcc gattcctcgc gcagccgcgc
     9421 ggccatgaag cggtcgcgct cgccgatcaa cgcttcgtag agcggcttgg attcacgcgc
     9481 gaattcgccg aaggcgcttt cgaggatgtc gccctgcttg agtttctcga tatcggcttc
     9541 atcgatttcc tcgcgcgaga acacgctcgc gatgagtccg ccgaggatac ccatgcggtc
     9601 gcgaaacggt accccgcgat aggcgtggtt gagggtgatg ccgatctccc ggtcgaccaa
     9661 ccacagcggc agcccgcgcg catcggcggc cgccatcgcg gccttcatct cggcgccggg
     9721 ctcgatgccg tattgttctg caatccgctg ctggaaggac gccagcgcca gcgaggcgaa
     9781 caccatgccg gccttgccgg tgcggatcac ctggaagagg tccatgttcg cgagcgcttc
     9841 gggatcgcgg atgccgcgtg cgcgcgattc gcaaagttcg atggcaatcg cgtcgaacgg
     9901 ctcgcgctcg accagcgcct tcaccgcatc caccgatgcg cgcgaaacgt gcgcggtgcc
     9961 gagcaacacg aattccacgc cgtcgcgcgt cacgcgcttg acgggctggt cttgcatcgg
    10021 gtcgggttgc gccgcgttct cggcggggga ctcggggccg gtgttcaatc gcggaaccgc
    10081 ctggtcaaat cgccgtaggc gtcgatgcgg cggtcgcgca ggaacggcca gatccgccgc
    10141 acgctttcgc tgcgttcgag gtcgacgtcc gcgatcagca gctcgcgctt gtcgctcccc
    10201 gcctgtgcga ggatttcgcc ctgcggcccg gcaacgaaac tcgagcccca gaactgcgcg
    10261 ccgcgtccgc cgtcgggtga cggctcgaag ccaacccggt tgcaggccgc gagcggcacg
    10321 ccgttggcca ccgcgtggcc gcgctgcacg gtgatccatg catcgcgctg gcggtccttt
    10381 tcgtcctgcg catcggcggg gtcccagccg atcgcggtcg gatacagcag gatctccgcg
    10441 cctgcgagcg ccatcaggcg cgccgcttcg gggtaccact ggtcccagca caccagcacg
    10501 ccgagcttgc cggccgacgt cgcgatcgga tggaaaccga ggtcgcccgg cgtgaaatag
    10561 aacttctcgt agtacgcggg gtcatcgggg atgtgcatct tgcggtaggt gccggcgatc
    10621 ccgaccgagc ggtcgaacac cactgcggtg ttgtgataca agcctgccgc gcgccgctcg
    10681 aacagcgaag cgacgaccac gagctccagt tcggcggcga gttttccgat ccgttcggtc
    10741 gacgggcccg ggatggtttc cgcgcgatcg aattccgcca ccgattcgtg ctggcagaag
    10801 taggggccgt tgtggatttc ctgcagcagc accagttgcg cgccggcccg cgcggcctcg
    10861 cgcaagcccg cctcgatcgc gtcgaggttg gcgtcggtgc tgccgcggtc ggtttcctgc
    10921 agcagcgcga gcttgagggt cttgttcgtc atgggcaaca gtgtacgcgg cgccttgccg
    10981 tcgcaggagc gcgcatggcg cgctcctaca agaacattac gccttcggga agctgcatcg
    11041 tcacgcagtg caggctgccg ttttgccaga tcagcggtcg gcaggggatc tgcaccactt
    11101 cacggccggg gtgggcgagt cggatggttt cggcggccgc gccgtcggcg atgtcgccgt
    11161 aggccggtac caggactgcg ccgttgatga ccaggtagtt cgcgtacgac gcggcgaggc
    11221 ggcggccttc ctcgacgatc ggcttcggcc acggcagcgg gaacaggcgg tacgggtcgc
    11281 cgccgagggt ccgcaaggcc tccaggtccc gtcgcatcat gtcgagttcg cggtaatgcg
    11341 gatcgtccgc gtcgctgcag gcctggtaca cgatcgcatc gtccggtgcg aggcgggcga
    11401 gggtgtcgat gtgcgcgtcg gtgtcgtcgc cttgcaggaa cccgtgctcg agccacagca
    11461 cgcgcgacgc ggacagcgcg ttgcgcaacg tgagttcgat ttgcccgcgt ggctcgtccg
    11521 ggtggcgcgt ttgcaggcac cgccaggtcg tcaggatcgt gccccggccg tcggattcga
    11581 tggcgccgcc ttccagtgcc caatccacgc gctggtgtct tgcgccggac cggaagacgc
    11641 cgcgctcgac caggcccgcg accagcgcgt cgtcctgcga ggcctcgaac ttgccgcccc
    11701 atccggtgaa ccggaaatcg accagcacga aaccaccggc cgcgtcacgc agggtgatcg
    11761 ggcccgaatc gcgaagccag gtgtcgtcgt aggggatctc gacgaaccgc acctgcccga
    11821 gatcggcccc cgcgtcgccc agcagggcgg ctgcgtgggc gccgatttca ccgtcgggaa
    11881 cgcagacgat caccggttcg aagcgggtga tcgctgcgac gagggccgcg tacgattcct
    11941 cgacctcgtc gagccgcgcg gcccagtcgg tgccggcgtt cggccaggcg agcaggatcg
    12001 ccgactgggg ctcccattcg gcgggcaggc gcaatccggg ggatggcatg atcgatggaa
    12061 ccttcaaagc ttccgatgtc gtcattgtgg cgcaggccgc acggcggggg cagagcctgt
    12121 cccggacttg atccgggaat gccgacgcgg acatccggcg aagccgatgg cccccacgag
    12181 ggctgccgca ggagcggcgc gcatcgcagg ccgcgtcggg cgccggacat ggatcccggc
    12241 cttggccggg acgacaggca aaaaacggga tcgcgcgcgg cttctacagc gaatcgggac
    12301 ccggcgtccg gagcaccgaa tggatcacgt gcttgtattc gaagtaaacg atgtacttcg
    12361 aatattccca gcggtggatc ggcggctggt acttggagct gccgccgcgg gtcgccagca
    12421 ctttcagtgg cgcgccgtat tcacgcttga cctggtccat ggtcatgccg cgcgtgggca
    12481 ggtccatgtg ctgctcctgc tgcaccttgg cgctcagcgg aacggactgc gtctgcgttt
    12541 gcaaggtgtc ggcctgcgcg ggcaatgcca gcacgccggc agcgagtgca aagatcacgg
    12601 cagtcgagct ggtggcgatc cggttcatgg ccatggcttc ccctggcgca gtgcgccgat
    12661 ggcgcatttg tagcaaaaac cgcagtcccg cgccagcgac gctgatcacg aaaaaggccg
    12721 cttgtgtgcg gcccatttcg gtgcaattgc gcggagcggc tgtcgtggaa ggtcagggca
    12781 ggatgcccgt tctggtgtcc gcgaatccga agccgtccag tttaccgctc ccccggcggc
    12841 gccgcggtca gggatgaccg cccacttatc tctggcgcgc cttgaaacgc ggattgctct
    12901 tgcagatcac gaaaaccttg ccgcgccggc gcacgatctt gcaatcgcgg tgccgctgct
    12961 tggcggactt cagggatgaa agcaccttca cgacgaactc ctcgggcgac gggaccggcc
    13021 gaaacaaagc gggaaagttt atcgcgcgcg gccatttgcc gcaagtccgc ggacatgctc
    13081 gatcgcggag gttatcctag cgggtccagc gcccagacga cccaggagcg aaccgccgtg
    13141 gctaccgacc tcgaccagac ctgcatcaac accatccgct tcctgtcggt ggacatggtg
    13201 cagaaggcca acagcggcca
//
LOCUS       DASSNH010000031         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_472623, whole genome shotgun sequence.
ACCESSION   DASSNH010000031 DASSNH010000000
VERSION     DASSNH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472623"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFK00_01115"
     CDS             complement(<1..209)
                     /locus_tag="VFK00_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591588.1"
                     /translation="MLRRDPSAWLACLLLLVVGSSASACPPSPQPWRAAIEQFVADDS
                     AHPPPQHAVLFVGSSSIRMWTALAR"
     gene            265..918
                     /locus_tag="VFK00_01120"
     CDS             265..918
                     /locus_tag="VFK00_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HET7591589.1"
                     /translation="MTTGSDNSVTFTLSPTGTPVTAPALRPFDLFPTRIWQARLPSLR
                     GQLGSWVAAALRMREADPAPAGRTNRHGWNSRDMGVLEQPVFAPLREAVHAGCASALR
                     EMGMPGLRFDLQSWINLHDRGGFNFLHVHEGCLLSGSFYLQVPPGSGPFVFRDPRPGV
                     VHGYVKGAVPNGYSDIRLAPEAGLLVLFPCWMEHFVEPHENDEPRIAIAFNAVTANP"
     gene            1014..3788
                     /locus_tag="VFK00_01125"
     CDS             1014..3788
                     /locus_tag="VFK00_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET7591590.1"
                     /translation="MIIRLRSAWLVIACICMPLAGLAATPGTSQVLEGKARFEFLTPS
                     LVRMEYAPSGHFVDAPTAVVERRDWPHVAVKSNRKNGWLVASTGAMTLRYRLQSGAFA
                     PANLEVKWTDADGTHSWHPGLVDDGNLGGLTYSLDNVSAKNLPPDVRDSPVHDIIPGI
                     DVLLPQARPGLLSRNGFAFIDDSRTPVWNAKAQWIGPRRSGGGQDWYLFTYGHDYPKV
                     LREYAELCGAIPMIPRYALGPMITDLNFEYLPGAAETKSSAFQNYGERHLQDEITRLR
                     RNGIPFDTLVLDFAWHDYGWQGGYDWSPLIPHPDRLSDWLHARGIKLSLNDHPGYANT
                     EESILSHDDSRAPAVLKALGKPLPPKPSFDMDLSGGWVFATDPRDEGIRQHWFAAGYD
                     DAKWKAIRTDAPLTAQGYARDVGVVWYRRRVQLPEHQPAHVYLYVSRQGKDYRLYVDS
                     RQVEHSHVRWPRRLTFADIGPYVHAGHPVQIAIRIEPNEYGSGFLRGALALSDVEPPP
                     AIRFDLSDKKQAEVFMRLLHGPLMQRGTDFWWVDGGSGAADMPGLDPQLWTNRVFYDY
                     SRKETGKRGFILARYGGWGSERYPAYFTGDTYSEWPVLAYEVAYSVRGGNVLIPYISH
                     DIGGFHGGKIDFDLYARWVEFGAFSPLLRMHSAHENPREGNLRMPWTYGERGVELARK
                     YFTLHTQLIPYIYTYTRIAHEESLPILRPLYLQHPDSAEAYRHPHEYWFGDELLVAPV
                     VAPGGDRTVWLPPGNWIGFFDGKRYEGGASFNAHYGVDQTPVFVRDGAIVPEQPADLA
                     SPKEKPLGRLVVNVYGSGNGKFDLYEDDGISLDYERHRYALTPMRYERGTDGVHRIVI
                     GPTTGAFDGQVQRRSYEVRIHGIAKPGRLSVEGKPAKGWTWNGADGTASATVPARDIR
                     DQIVVEWRHP"
     gene            complement(3945..>4364)
                     /locus_tag="VFK00_01130"
     CDS             complement(3945..>4364)
                     /locus_tag="VFK00_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HET7591591.1"
                     /translation="ALFKAFDAPIRDYIARIGHGSDPLPRRNLGGYRFNGSWSVRLRA
                     SGFHHNHVHPRGWISSACYVALPEGMVNPADRGGTLTFAEPGILTTPPLPAEHEVRPE
                     PGTLVLFPSYFWHGTVPFSGERMRLTVAFDAVPAPGM"
BASE COUNT          768 a         1449 c         1433 g          714 t
ORIGIN      
        1 tcacgggcga gcgcggtcca catccggatc gaggaactgc cgacgaacag cacggcgtgt
       61 tgcggcggcg ggtgcgcgct atcatccgcg acgaactgct cgatggccgc acgccacggc
      121 tgcggtgacg gcgggcacgc cgacgcgctg cttccgacga ccagcaacag aagacacgcc
      181 aaccatgccg atggatcgcg ccgcaacatg cagctgcccc ggtggccaat gggtcgcgat
      241 ccatgttgaa ccccggaagg ctccgtgaca accggtagcg acaacagcgt aaccttcacg
      301 ctgtcgccaa ccgggactcc cgtcaccgcc cccgcgctgc gccctttcga cctcttcccg
      361 acccggatct ggcaggcacg gttgccctcg ctgcgcggac aactcggctc ctgggtggct
      421 gcggcgctgc ggatgcgcga agccgacccc gcgcccgccg gtcgcaccaa tcgccatggc
      481 tggaacagcc gcgacatggg cgtgctggag cagccggtgt tcgcaccgct gcgcgaggcg
      541 gtgcacgcgg gctgcgccag tgcgctgcgc gaaatgggaa tgcccggcct ccggttcgac
      601 ctgcaatcgt ggatcaacct gcacgaccgc ggcggattca acttcctgca cgtgcacgaa
      661 ggctgcctgc tgtcgggttc gttctacctg caggtgccgc cgggctccgg acccttcgtg
      721 ttccgcgatc cgcggcccgg cgtggtgcat ggctacgtga aaggtgcggt gccgaacggt
      781 tacagcgaca tccggctcgc gccggaagcc ggactgctgg tactgttccc gtgctggatg
      841 gaacatttcg tcgagccgca cgaaaacgat gaaccccgca tcgcgatcgc cttcaatgcg
      901 gtgacggcga atccctgacc gcggcgaaga gggctccggc ggggagcgac acaagcgcct
      961 cggggcgtgc acgacgagcg gacgttcctt cgcatcagca ttgggggtgc cgcatgatca
     1021 ttcgtttgcg ttcggcgtgg ctggtgatcg cgtgcatctg catgccgctg gcgggcctcg
     1081 ccgcgacacc cggcacctca caggtcctgg agggcaaggc gcgcttcgag ttcctgacgc
     1141 catcgctggt caggatggag tacgccccgt ccggccattt cgtcgatgcg ccgaccgccg
     1201 tcgtggagcg gcgcgactgg ccgcacgttg cggtgaagag caacaggaag aatggttggc
     1261 tggtcgcaag tacgggcgcg atgaccttgc gctaccggct gcagtccggc gcgttcgcgc
     1321 cggccaatct cgaggtgaag tggaccgatg cggatggcac ccattcctgg catcccgggc
     1381 tggtcgatga cggcaatctc ggcggcctga cctattcgct ggacaacgtc agtgcgaaga
     1441 atctgccgcc cgacgttcgc gacagtcccg tccatgacat catccccggc atcgacgtgc
     1501 tgctgccgca agcccggccc ggcctgctca gccgcaacgg tttcgcgttc atcgacgaca
     1561 gccgtacacc ggtctggaat gccaaggccc aatggatcgg accgcgcagg tcgggcggcg
     1621 gacaggactg gtacctgttc acctacggcc acgattaccc gaaggttctt cgcgagtatg
     1681 ccgagttgtg cggtgcgatt ccgatgatcc cgcgctacgc gctgggcccc atgatcaccg
     1741 acctcaactt cgaatacctt cctggcgccg ccgaaacgaa gtcatccgcc ttccaaaact
     1801 atggggaaag gcacctccaa gacgagatca cccggcttcg ccggaacggc atcccgttcg
     1861 acaccctggt cctggatttc gcctggcacg actacggctg gcagggcggc tacgactgga
     1921 gtccgttgat cccgcacccg gaccggctct cggactggtt gcacgctcgc ggcatcaagc
     1981 tgtccctgaa cgaccatccg ggttacgcga acaccgaaga aagcatcctg tcgcacgacg
     2041 acagccgcgc gccggccgtg ttgaaggcgc tgggaaaacc gcttccaccc aaaccgtcgt
     2101 tcgatatgga tttgtccggc ggttgggtat tcgccaccga tccacgcgat gagggcattc
     2161 ggcaacactg gtttgcggcc ggctacgacg atgcgaaatg gaaagcgatc cgcacggatg
     2221 cgccattgac ggcgcaggga tacgcaaggg atgtcggcgt cgtgtggtat cgccgccgcg
     2281 tgcaacttcc tgaacatcag ccggcgcacg tttatctgta cgtgagccgc cagggcaagg
     2341 actaccggct ttatgtcgac agccgccagg tcgaacacag ccacgtgcgc tggccgcggc
     2401 gtctgacttt cgccgacatc gggccgtacg tgcatgcggg ccacccggtc cagatcgcca
     2461 tccgtatcga gccgaacgag tacggcagcg gcttcctgcg tggagcgctc gcgctcagcg
     2521 acgtcgaacc gccgccggcg atccggttcg acctctccga caagaagcag gcggaggtgt
     2581 tcatgcggct cctgcacgga ccgctgatgc aacggggcac cgacttctgg tgggtggacg
     2641 gcggcagcgg cgcggcggac atgccgggcc tcgacccgca gctctggacc aaccgtgtct
     2701 tctacgacta ctcccggaag gaaaccggca agcgcggctt catcctcgcc cgctacggcg
     2761 gctggggcag cgaacgctac cccgcgtatt tcaccggcga cacctattcc gaatggccgg
     2821 tgctggccta cgaggtcgcc tacagcgtgc gcggcggcaa cgtgctgatc ccgtacatca
     2881 gtcacgacat cggtggattc cacggcggca agatcgactt cgacctttat gcacggtggg
     2941 tggagttcgg tgcgttcagt ccactgctgc ggatgcacag cgcacacgag aacccgcgcg
     3001 aaggcaacct gcggatgcca tggacctacg gcgaacgcgg cgtcgagctg gccaggaagt
     3061 acttcacgct gcacacgcaa ctcatcccct acatctatac ctacacccgg atcgcgcacg
     3121 aggaatcgct gccgatcctg cgtccgctgt acctgcagca tccggattcc gcggaagcgt
     3181 atcggcatcc gcacgaatac tggttcggcg acgagctgct ggtcgcgccg gtcgtcgcac
     3241 ccggcggcga ccgcacggtc tggttgccac ccggcaactg gatcggcttc ttcgacggaa
     3301 agcgttacga gggcggcgcg tcgttcaacg cgcactacgg cgtggaccag accccggtct
     3361 tcgtacgcga cggcgcgatc gttcccgaac aaccggctga tctcgcctcg ccgaaggaaa
     3421 agccgctggg acgccttgtc gtcaacgtgt acgggtcggg caacggcaag ttcgacctct
     3481 acgaagacga cggcatttca ctggattacg aaaggcatcg ctacgcgctg acgccgatgc
     3541 gctacgagcg tgggaccgat ggggtgcacc gaatcgtgat cggtcccacc acgggtgcct
     3601 tcgacggcca ggtgcaacgg cgctcctacg aggtccggat ccacggcatc gccaaaccgg
     3661 gccggttatc ggtggagggc aagcccgcca agggctggac ctggaatggc gcggatggca
     3721 ccgcatcggc gacggtgccg gcgcgcgata tccgcgacca aatcgtggtc gagtggcggc
     3781 acccgtgatg cagggcggac gcgctccgcc ttgatcgggg gcgcggcggg cgatgtccgc
     3841 cctgcgatga cttcgataca gggccggccg gtagcccgga tggagcgaag cgggatccgg
     3901 ggctccttgt cgcactcctg gaccggcact gcgcgcctgt cgggctacat cccgggcgcg
     3961 ggtaccgcgt cgaacgccac ggtcagccgc atccgctcgc cggaaaaagg caccgtgcca
     4021 tgccagaaat aggacggaaa cagcaccagc gtgcccggct ccggacgcac ttcgtgttcc
     4081 gcgggcaggg gcggagtggt gaggatcccg ggctcggcga aggtcagcgt ccctcccctg
     4141 tcggccggat tgaccatccc ttcgggcagg gcgacgtagc acgcggatga aatccatccg
     4201 cgcgggtgca cgtggttgtg gtggaacccg ctggcgcgca gccgcaccga ccagcttccg
     4261 ttgaagcgat agccgcccag gtttcggcgc ggcaatggat cggaaccatg gccgatgcgg
     4321 gcgatgtagt cgcggatcgg cgcgtcgaaa gccttgaaca gcgc
//
LOCUS       DASSNH010000032         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1607710, whole genome shotgun sequence.
ACCESSION   DASSNH010000032 DASSNH010000000
VERSION     DASSNH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607710"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFK00_01135"
     CDS             <1..748
                     /locus_tag="VFK00_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010373696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7591592.1"
                     /translation="LAEQVEHAITHSGLRDYYERDSRGNAEARVENLDELVNVASRFE
                     PTPEDIEAGLSELAAFLAHAALEAGEGQGAAGEDCVQLMTLHSAKGLEFPVVFLVGLE
                     DGLFPTQRSVEDPSRLEEERRLAYVGITRARHRLVLCYAESRRQYGSETLSRPSRFLD
                     ELPAHLLNEVRPRAKISRPVYTTRASRGHAELDAGPAIKLGQRVRHASFGEGVVVSAE
                     GSGAHTRIQVNFANAGPKWLVAAYANLTAL"
     gene            complement(983..1909)
                     /locus_tag="VFK00_01140"
     CDS             complement(983..1909)
                     /locus_tag="VFK00_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7591593.1"
                     /translation="MNDRQALTVPMRAKPTVALALSTGGARGLAHIGAIEAIDAAGYR
                     IVGVSGSSMGALIGGIYAAGKLDVYREWVMALQKMDVLKLVDWTLSGAGLIKGERIIG
                     VLRELIGDVDIEELPMAFTAVATDLDREREVWLTRGSLFDAIRASIAIPTIFRPHPLH
                     GHRLVDGGLMNPLPLTPLLQSPSDYTMAINANGPPESLPSAPATAPKPRRGLRSKIAA
                     YVTRTIASHVETGSEPGWTATLTQALDLMQENLTSFRLAADRPDLVIEVPRNASAVYE
                     FYRASELIDLGRDRAQRALAAWQPAHLHPEQE"
     gene            complement(1906..2886)
                     /locus_tag="VFK00_01145"
     CDS             complement(1906..2886)
                     /locus_tag="VFK00_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET7591594.1"
                     /translation="MDRYERILTLHRIFQSHRLPVGVERLREELDCSRATLYRDLAFL
                     RDALGAPLDSDPEGRGYRYAEGEAGRFELPGLWLTSEELSALVALETLVRRSDPGVLA
                     DALAPFRERVEKLLNAHSGTTKQPMERIRVVPWGSRKLNQQVFRTVASAVLARRQLKF
                     RYRARTTGADSVRHVSPQRLTHYRDNWYLDAWDHDREALRSFAVDRIGDAEQLDQPAI
                     DRDEKELNETLASSYGIFAGAPKAWATIRFSARAARWVADEHWHSLQEGRWLDDGRYE
                     LRVPYAQSRELVMDILRYGPDAEVVAPQSLREEIRIMHKLALDGYEQSSP"
BASE COUNT          472 a          986 c          988 g          481 t
ORIGIN      
        1 gctggcggaa caagtcgaac acgcgatcac gcattccggc ctgcgcgact actacgagcg
       61 cgacagccgc ggcaacgccg aggcgcgggt cgaaaacctg gacgagctgg tcaatgtcgc
      121 cagccgtttc gagccgacgc ccgaggacat cgaggccggg ctgtcggaac tcgcggcgtt
      181 cctcgcgcat gccgcgctgg aagcgggcga aggccagggt gccgcgggcg aggattgcgt
      241 gcagttgatg acgctgcatt ccgccaaggg cctggagttc ccggtcgtgt tcctggtggg
      301 cctggaagac ggcctgtttc ccacccagcg ctccgtggag gatccttcgc ggctcgagga
      361 ggaacgccgg ctcgcctacg tcgggatcac ccgcgcgcgc caccggctgg tgctttgcta
      421 tgcggaatcg cgccgccagt acggcagcga aacgctgtcg cggccgtcgc gcttcctgga
      481 cgaactgccc gcgcacctgc tgaacgaagt gcgcccgcgc gcgaagatat cgcggccggt
      541 atacacgacg cgcgcttcgc gcggccatgc cgaactggat gccggcccgg cgatcaagct
      601 cggccaacgc gtgcgccacg cgagcttcgg cgaaggcgtg gtagtcagcg cggaaggctc
      661 cggcgcgcac acccgaatcc aggtgaactt cgccaacgcc ggccccaaat ggctggttgc
      721 cgcctacgcg aacctcacgg cgctgtaggt tgggttgatc cggacttgtt ccgggagaag
      781 cccaacgatg gcgaacacgt tcggcttcgc tgcgctcggc gccaacctac aagagcgctc
      841 agcccaggcc tgcggttcag taggttgggt tgatcccgga cttgttccgg gagaagccca
      901 acagtcgttg cgtaagtgtg aatcgcggta cttcgttggc ttccccgcgg atccggcccc
      961 aggtccgcgc caatctgcaa gctcattcct gctcgggatg caaatgcgcc ggctgccaag
     1021 ccgcgagcgc acgctgcgcc cggtcgcgtc ccaggtcgat cagttcggag gcgcggtaga
     1081 actcgtagac cgcgctggcg ttgcgcggca cctcgatcac gaggtccggc cgatccgcgg
     1141 cgagccggaa actggtgagg ttttcctgca tcaggtccag cgcctgcgtg agcgtggcgg
     1201 tccagcctgg ctcgctgccg gtttccacgt gcgaagcgat ggtgcgtgtc acgtaggccg
     1261 cgatcttgct gcgcagtccg cggcgcggtt tcggggcggt cgccggagcg gatggtaggg
     1321 attcgggcgg gccgttcgcg ttgatcgcca tcgtgtaatc gctgggcgac tgcagcagcg
     1381 gtgtcagcgg cagcgggttc atgaggccgc cgtccaccag acgatggccg tgcagcggat
     1441 gcgggcggaa gatcgtggga atcgcgatcg aggcgcggat cgcgtcgaac agggatccac
     1501 gcgtgagcca gacttcgcgt tcgcgatcca ggtccgttgc gaccgcggtg aacgccatcg
     1561 gcagttcctc gatatccaca tccccgatca gttcgcgcag cacgccgatg atgcgttcgc
     1621 ccttgatcag cccggcgccc gacagcgtcc aatcgaccag cttgagcaca tccatcttct
     1681 gcagcgccat cacccactcg cggtacacat cgagtttgcc ggcggcgtag atcccgccga
     1741 tcagcgcccc catcgaactg cccgagacgc cgacgatccg gtaaccggcg gcgtcgatcg
     1801 cttcgatcgc gccgatgtgg gcgaggccgc gggcgccgcc ggtgctgagc gccagcgcca
     1861 cggtcggctt ggcgcgcatc ggcaccgtga gcgcctggcg gtcgttcacg gcgacgattg
     1921 ctcgtagccg tcgagcgcga gcttgtgcat gatccggatt tcctcgcgca gcgactgcgg
     1981 cgccaccact tcggcgtcgg gaccgtaacg caggatgtcc atcacgagtt cgcgcgattg
     2041 cgcgtagggc actcgcaatt cgtagcggcc gtcgtccagc caccggccct cctgcaagga
     2101 atgccagtgc tcgtccgcga cccaacgcgc ggcgcgcgcg gagaatcgga tcgtggccca
     2161 cgccttgggc gcgcccgcga agatgccgta gctcgacgcg agcgtctcgt tcaactcctt
     2221 ttcgtcgcgg tcgatcgcgg gctggtcgag ctgctcggca tcgccgatgc gatccaccgc
     2281 gaaactgcgc agggcttcgc ggtcatggtc ccacgcatcc aggtaccagt tgtcgcggta
     2341 atgcgtaagc cgctgcggcg aaacgtgccg cacgctgtcg gctccggtgg tgcgcgcgcg
     2401 gtagcggaac ttcagttgcc ggcgcgccag caccgcgctg gccaccgtgc ggaacacctg
     2461 ctggttgagc ttgcgcgaac cccagggcac caccctgatc cgctccatcg gctgcttggt
     2521 cgtacccgaa tgcgcattga gcagcttctc gacgcgctcg cgaaacgggg ccagcgcatc
     2581 ggccagcaca ccggggtccg aacgccgcac cagcgtttcg agcgccacca gtgccgagag
     2641 ttcctcgctg gtcagccaaa gcccgggcaa ctcgaagcga cctgcctcgc cttccgcgta
     2701 gcggtaacca cgcccttccg gatcgctgtc cagcggcgca ccgagcgcgt cgcgcaggaa
     2761 tgcgaggtcg cgatacaagg tcgctcgcga gcaatccagt tcctcgcgca ggcgttcgac
     2821 gccgaccgga agccggtgcg actggaaaat ccggtgcagg gtgagaatgc gctcgtagcg
     2881 atccatccgc gcagcatgcc gcgcagtgcg attggcggca agcgcga
//
LOCUS       DASSNH010000033         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_470716, whole genome shotgun sequence.
ACCESSION   DASSNH010000033 DASSNH010000000
VERSION     DASSNH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470716"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..1566)
                     /gene="wecB"
                     /locus_tag="VFK00_01150"
     CDS             complement(400..1566)
                     /gene="wecB"
                     /locus_tag="VFK00_01150"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET7591595.1"
                     /translation="MHRLKIDLIAAARPNFMKVAPLFHALRDEAWCSLRLVHTGQHYD
                     RNMSDAFFQDLGLPLPDLHLGVGSGSHAEQTANVMLAYERACNEGRPDLIVVVGDVNS
                     TLACTLVGSKLHIPVAHLEAGLRSGDRRMPEEINRLVTDTLADILWTPSPDGDENLAA
                     EGVPADKITRVGNIMIDSYELRRARIEASGERGKLGLEDRRYAVVTLHRPSNVDEQAP
                     LAALVRQLTNIAAKLPLVFPVHPRTRKNLETFGLWTELADAPGMQLIEPLGYVDFMNL
                     VCGARLVITDSGGVQEETTYLDIPCLTLRTTTERPITITQGTNRLLGASELAMAVEEV
                     LAGCWPHGRKPDLWDGHTAARVAADIRRRLLDGQVTGVEADDVAETPVRHAVGN"
BASE COUNT          341 a          661 c          672 g          326 t
ORIGIN      
        1 gccgaccatc agcagcgccg agccgacaag gaatgcggcc gtggcagtca ggtagctggc
       61 gatgacgatc aagtgcatgc gaagtcgaac ccctgtcgcg aggtgctggt cagcgtccca
      121 tcaaccgcaa agtgtccgga aggcgtgaac ccgctccgcg tgccgaaggg cgggagaaat
      181 cgcccccgaa ggggtcgccg cgaagcggcg gggggatgtg cgcgaccgaa aggccatccc
      241 cctcgatccc ccttcgttcc gaagggggag ggaaaagcta cccccttcgg aacgaagagg
      301 gtcggtgcgg agctcgaata cgaagcgctg gtgctccgcg ccggggggat gcaggttgcc
      361 gcggatcaaa ccggccatgg ggttcgcccc tcgtcctgat cagttgccca ctgcgtggcg
      421 cacgggcgtt tcggcaacat cgtccgcttc gacaccggtg acctgcccgt ccagcaggcg
      481 gcgccggatg tcggccgcca cgcgcgccgc ggtgtggcca tcccacaaat cgggcttgcg
      541 tccgtgcggc cagcaaccgg cgaggacttc ctccactgcc atcgccagct cgctcgcgcc
      601 cagcagccgg ttggtgccct gggtgatggt gatcggccgt tcggtggtgg tgcgaagcgt
      661 caggcagggg atgtcgaggt acgtggtttc ctcctgcacg ccacccgagt cggtgatgac
      721 cagacgcgcc ccacagacga ggttcatgaa atccacgtag cccagcggct cgatgagctg
      781 catgccgggt gcatcggcga gctccgtcca caggccgaaa gtttccaggt tcttgcgggt
      841 gcgcggatgc accggaaaca ccagcggcaa cttggccgcg atgttcgtca actgccgcac
      901 cagtgccgcc agcggtgcct gctcgtccac gttcgacggg cggtgcaggg tcaccaccgc
      961 gtagcggcgg tcttccaggc ccagcttgcc cctttccccg ctcgcttcga tgcgcgcacg
     1021 cctcaattcg taggagtcga tcatgatgtt gccgacccgg gtgatcttgt ccgccggcac
     1081 gccttcggcg gccaggttct cgtcgccgtc gggcgacggc gtccacagga tgtccgcaag
     1141 ggtgtcggtc accagccggt tgatctcttc gggcatccgc cggtcgccgc tgcgcagccc
     1201 agcctcgagg tgtgccacgg gaatgtgaag cttcgacccg accaaggtgc aagcgagcgt
     1261 ggaattgacg tcgccgacca ccacgatcag gtcgggccgg ccttcattgc aggcgcgctc
     1321 gtaggccagc atcacgttgg cggtttgttc ggcatggctg ccgctgccga cgccgagatg
     1381 caggtccggc aaaggcagcc ccagatcctg gaaaaaggcg tccgacatgt tgcggtcgta
     1441 gtgctgcccg gtgtgcacca gccgcaagct gcaccacgcc tcgtcgcgca acgcatggaa
     1501 caacggcgcg accttcatga agttgggccg cgccgcggcg atcaagtcga ttttcagccg
     1561 gtgcatggca agccggtctc ccgcattccg taggcggtcg agtgtagcaa gcgcccgccc
     1621 gtcgggcgga tccatggcta ttgtcatgca tgacacgcgc cagatgggcg ggccaggccg
     1681 gcgcgcgcaa gcggcgctcc gcccgtccgc gttgcaggtt gggctgatcc cgggcttgtc
     1741 ccgggagaag cccaacatga ctcggaaaaa gatcttcgga cccgcgctcg agacgggtcg
     1801 atctctcgcc ccttggggct ggtttggaag cggcgggcct ctcaaaaaaa caacccccgt
     1861 cgccgggggt tgtcgaagag cgctcaaaga tcgttcgtca gctttggaac gaacgccgcc
     1921 gcagacccgc gaacaggccg accagcagca cgcccaggcc gaacatgccg agcgccgcgg
     1981 gttccgggac gccattgacg
//
LOCUS       DASSNH010000034        11294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1105841, whole genome shotgun sequence.
ACCESSION   DASSNH010000034 DASSNH010000000
VERSION     DASSNH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105841"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="rpsF"
                     /locus_tag="VFK00_01155"
     CDS             <1..379
                     /gene="rpsF"
                     /locus_tag="VFK00_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7591596.1"
                     /translation="DQSEQVPGMLDRYKALVEGEGGKVHRVEDWGRRPLAYPIQHLAK
                     AHYALLNIECGPKAIDELKSGFRFNDAVLRHLIMQWHCAETEQSLILKTKDKDDKPSR
                     RRDDDDDDDRGDRNDRNDRSEAA"
     gene            418..648
                     /gene="rpsR"
                     /locus_tag="VFK00_01160"
     CDS             418..648
                     /gene="rpsR"
                     /locus_tag="VFK00_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7591597.1"
                     /translation="MSKFFRRRKYCRFSAEGIEHIDYKDLATLKQYVTETGKIVPSRI
                     TGTKARYQRQLATAVQRARFLALLPYSDNHFV"
     gene            825..1274
                     /gene="rplI"
                     /locus_tag="VFK00_01165"
     CDS             825..1274
                     /gene="rplI"
                     /locus_tag="VFK00_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7591598.1"
                     /translation="MELILLEKVKNLGALGDKVKVKPGFGRNYLVPQGKAVPANKANL
                     EAFEAKRAEYQAKADKLLGDAQARAAKFENAEATLSANASPEGKLFGSVGPREIAEAF
                     TRQGLELEKSEVIMPEGAIHNTGDYEVEISLHADVKAPVKVHVVAES"
     gene            1549..1983
                     /locus_tag="VFK00_01170"
     CDS             1549..1983
                     /locus_tag="VFK00_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HET7591599.1"
                     /translation="MIRVSRLADYASAVMASLARHPGEVLPAAAVAEETRLEMPTASK
                     LLKRLARAGLVESFRGAAGGYRLARAATDISLAEIVEALDGPIGLTACRIGRTGCARQ
                     PYCTVSHGWQGVGAAIDSALRAVSLADMAQRQPARVHGARMA"
     gene            1994..3433
                     /gene="sufB"
                     /locus_tag="VFK00_01175"
     CDS             1994..3433
                     /gene="sufB"
                     /locus_tag="VFK00_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7591600.1"
                     /translation="MATQPSEVQAALSRRYEAGFVTDIESLSLPPGLDEDTIRALSAL
                     KEEPDWMTEWRLSAYRHWLTMKQPDWAHVNLAPIDFQAISYYSAPKNKPKSLDEVDPK
                     LLETYDKLGVPLHERAKLAGVAVDAVFDSVSVANTAREQLAKVGVIFCSMSEAIREYG
                     DLVRQYLGTVVPQGDNFFAALNSAVFSDGSFVFIPKGVRCPMELSTYFRINAGHTGQF
                     ERTLIIAEEDSVVSYLEGCTAPMRDDNQLHAAVVELVALERASIKYSTVQNWYPGDEN
                     GKGGIYNFVTKRGECRGDDSRITWTQVETGSAITWKYPSCVLRGDRSVGEFHSVALTH
                     HYQQADTGTKMIHIGRDTKSKIVSKGISAGRSQNSYRGLVKVEKGAANARNHTRCDSI
                     LIGKKCGSHTYPYVEVKNPTAIVEHEASTSKISDDQLFYCRSRGIGEEDAVSMIVDGF
                     CKQVFRELPMEFAVEAKKLLEVSLEGAIG"
     gene            3593..4357
                     /gene="sufC"
                     /locus_tag="VFK00_01180"
     CDS             3593..4357
                     /gene="sufC"
                     /locus_tag="VFK00_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7591601.1"
                     /translation="MLKIENLHARVAGKEILKGLSLEVKAGEVHALMGPNGAGKSTLG
                     NVLSGRPGYEVTEGIVTFDGANLLDMAPEERAAAGVFLAFQYPVEIPGVNNTYFLRAA
                     LNAQRKSRGEEELDSMQFLKLVRGKVKEMGMTPELLNRAVNEGFSGGEKKRNEVFQMA
                     VLQPRLAILDETDSGLDIDALKQVAEGVNALRSPGRAFLVITHYQRLLDYIVPDFVHV
                     LDGGRIVESGDARLAQDLEAHGYAWVRDRMPAGAQA"
     gene            4363..5670
                     /gene="sufD"
                     /locus_tag="VFK00_01185"
     CDS             4363..5670
                     /gene="sufD"
                     /locus_tag="VFK00_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7591602.1"
                     /translation="MPAPFVESMRAAAAALRLPGSGLAWLDAARCEALQAFVERGLPT
                     ARSELWKYTALRALAQRQYAAGDGEAAGRTIDDALLPAHAGARVVFVNGVFRADLSRL
                     DVLPDGVVLRSLAAALREQPEPLRFLLGPHKDDADDGFTLLNRALAADGVILRVAPGV
                     QVVEPVHVVHVGVAAQQAVACHCRSLIELGEGARLGIVEHHVGEAAAGHLVNLVRKVK
                     LHDRARLDWVGIQSSPADATLLRRSDVVLHDRAQLDFHALELGGKLARHELRIDLAGA
                     GARFRSRGAFVLHGRQHADTEVLVTHRGRDTASDSLWRGVADDRARGVVHGRILVMPG
                     ADGADGSFYNKNLLLSASAEIDTRPALEIYADEVKANHGATVGQLDENVLFYLRSRGI
                     PLDVARRMLVQAFCAVTLQGIEPAPLRERCETLLVEQLPAVAA"
     gene            5667..6923
                     /locus_tag="VFK00_01190"
     CDS             5667..6923
                     /locus_tag="VFK00_01190"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7591603.1"
                     /translation="MNANPESAAVTASVVDWSRVREDFPILARKVHGKPLVYLDSANT
                     SQKPRQVIEAVDAFYREHNANVSRAVHQLSEEATALYEGARTKLASFLGANHREEIVL
                     TSGTTMATNLVAYSYLLPRLKPGDAVLVTAMEHHADIVPWQLVCKLTGAHLKVAPVND
                     AGELIIGKFMEALTPEVKFAGVVHVSNVLGTINPVKELARECRRRGIPLLVDGSQAAP
                     HLPLDVKSIGCDFYCVTGHKMCGPTGTGALWARRELLETMPPFFGGGEMIKTVSFDGT
                     TFADSPRKFEAGTPNTAGFAGLGAAIDYLSGIGMDNVAARERTLLDYATPRLQAVPGL
                     RIFGTSPHKAAVISFLIDGVHAHDLATLLDQEGIAVRSGHHCAHPLMQFYGVAATCRA
                     SLAFYNTESEIDAFVDAIGRVRKLLA"
     gene            6920..7531
                     /locus_tag="VFK00_01195"
     CDS             6920..7531
                     /locus_tag="VFK00_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7591604.1"
                     /translation="MKNGDRGPGTGGREPHRSGAHAALVHRPLCFRSATEADVEAVVA
                     LTQSAYRGDQSRVGWTTEADLLDGQRTDAREVGELIANPDVKLLLAERDGRLLACCVV
                     ERSANAHPGEGYFGMFSVRPDAQGNGTGRALLAEAERVARDDWHCRAMRMTVIDCRAE
                     LIAWYERRGYRRTGEHRPFPYGDPRFGIPKRDDLRFEWLVKKL"
     gene            7651..7971
                     /locus_tag="VFK00_01200"
     CDS             7651..7971
                     /locus_tag="VFK00_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET7591605.1"
                     /translation="MSEEWVKVCAQDELLPGEFRVVWDEDTAIAVYNLEGDLYAIEDT
                     CTHDGGELAGGEVCGFEVECPRHGARFDVRTGEVKQAPATEPIASFPVRVEEGIIYTR
                     DDRS"
     gene            complement(8094..9473)
                     /locus_tag="VFK00_01205"
     CDS             complement(8094..9473)
                     /locus_tag="VFK00_01205"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HET7591606.1"
                     /translation="MVRNPSLRRRLLMFVSVPLLAVLICGGIINYVIGLHYANKVYDR
                     WLLNSANAMGDLVNSDAGRNELSEQARILLQFSVHDRNIFAVRSLRYGVLAGDQDLPQ
                     PMPDGSGGGSVFTNFSDHGQAMRMASVFLPNKADPGDTLVISTAETVRNRKALARELL
                     FSTAPIELLLICVAMALVWLGINRGLRVLDPLAHEIRMRDAGDLSPLRTIDAPIEVQP
                     LVATIDALLSRLDRMLRQQQRFIADAAHQLRTPLAGLRLQAERALADPRPDTIREALT
                     HVERLSAGTARAAGQLLALARAQAPDESLGVATPLDLANVVRDEVASRVPEAIARGID
                     LGYQGPDRGVTIIGDAVLLREMLGNLIDNAMRYGTRGDPAITVGLQPDDGRGCVLSVQ
                     DNGPGVAPELVPRLGERFFRAIGNGHEGTGLGLAIVREIAERHGAELSFINLRDPHGL
                     RAEIRFPAN"
     gene            complement(9463..10137)
                     /locus_tag="VFK00_01210"
     CDS             complement(9463..10137)
                     /locus_tag="VFK00_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7591607.1"
                     /translation="MRMLVVEDDAAIADAVCDSLERAGHAVDRLSNGKAAIAALQEHA
                     FGLVVLDLGLPGQDGSEVVRRLRTSHDGVPVLVITAREEVDLRVRTLDLGADDYLVKP
                     FSLAEFDARVRALLRRQGNRGMPVLHLGKLTIDLAGRRVLQGDQPLELTAREFALLEV
                     LASRRNRVTPREHVIEALCTWNDALTDNGLDIAVHRLRRKLAESDVTLRTVRGLGYLL
                     EADDGS"
     gene            complement(10166..10738)
                     /locus_tag="VFK00_01215"
     CDS             complement(10166..10738)
                     /locus_tag="VFK00_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HET7591608.1"
                     /translation="MSILRAIPRSAQREEPWANGAGSTTVLLREPDAAEWTVRISIAN
                     VDAPGPFSELPDTRRVLVPLDAPMGLRFPDGRDLAAPRFRTLRFDGSPAPAGLLPEGP
                     TRDFNLMLRGGARGDVLPRTLVDSMVLPSLPGVRWLVYLDSGRSRVDAGGGGSLALEP
                     REAVLVATGAAPERVLVEGAGEIVLVKLYA"
     gene            complement(10769..>11294)
                     /locus_tag="VFK00_01220"
     CDS             complement(10769..>11294)
                     /locus_tag="VFK00_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET7591609.1"
                     /translation="PPPPPPPALHSRAFPGASSLSVILPRYHIAPSLIPGAGQGVFLD
                     EPLRHGRIAIAPDRIDGTWSFAEIMADPERAALMHTSVRWFEDRYTLSPDWPDDCFVN
                     HSFEPSGLWVLGFIFAARDLEPGEELTVDYRHLLAPGQEESFRDAHTGEAIVGYPWRD
                     SLRLGIESLQKLLD"
BASE COUNT         1945 a         3643 c         3891 g         1815 t
ORIGIN      
        1 ggaccagagc gagcaggtgc ccggcatgct cgaccgctac aaggccctcg tcgagggcga
       61 aggcggcaag gtgcaccgcg tcgaggactg gggccgccgc ccgctcgcct atcccatcca
      121 gcatctggcc aaggcccact atgccctgct caacatcgag tgcgggccaa aggcgatcga
      181 cgagctgaag tcgggcttcc ggttcaacga tgcggtgctg cgccacctga tcatgcagtg
      241 gcactgcgcg gaaaccgagc agtccctgat cctgaagacc aaggacaagg acgacaagcc
      301 gtcccgtcgt cgcgatgacg atgatgacga tgaccgcggc gatcgcaatg accgcaacga
      361 ccgtagtgaa gccgcctgat gcgcggccaa gccagaaaga agactggagc tcccaacatg
      421 tccaagtttt tccgccgtcg caagtactgc cgcttcagcg ccgagggcat cgagcacatc
      481 gattacaagg atctcgccac gctcaagcag tacgtcaccg aaaccggcaa gatcgttccc
      541 agccgcatca ccggcaccaa ggcgcgctac cagcgccagt tggccaccgc ggtgcagcgg
      601 gcccgtttcc tcgcgttgct gccttacagc gacaatcatt ttgtttgatc gcttgtcccc
      661 tctccctccg ggagatggtg gcgcgcagcg ccgggcgagg gtacgggttc gcagcagcgt
      721 cccgtgctcg cctccggctc actccgctcc gcgacgattc ctcaaattta ttcggacaac
      781 cacaggctgc gtctcgacgg ggcgctaacg aatcggagaa tttcatggaa ctcatcctgc
      841 tcgaaaaggt gaagaacctc ggcgccctcg gcgacaaggt caaggtcaag cccggcttcg
      901 gccgcaatta cctggtgccg caaggcaagg ccgtccccgc caacaaggcc aatctcgaag
      961 ctttcgaggc caagcgcgcc gaataccagg ccaaggccga caagctgctg ggcgacgccc
     1021 aggcgcgcgc ggcaaagttc gagaacgccg aggcgaccct cagcgccaat gccagccccg
     1081 aaggcaagtt gttcggctcg gtcggccctc gcgaaatcgc cgaggcgttc accaggcagg
     1141 gactcgagct cgagaagagc gaagtgatca tgccggaagg cgcgatccac aacaccggcg
     1201 actacgaggt ggaaatctcg ctgcacgccg acgtcaaggc accggtgaag gtccacgtgg
     1261 ttgcggaatc ctgatcgcga cggacttgtc gatccggaaa cgggcgccgc gtgcgcccgt
     1321 ttccttttca gccgttgtcc ctgccgaacc gccaggacgg gcgcgccggc ttctcaacct
     1381 gtcggcatac gagctttttg acacggattt acgcggatgc gcccggacgg agccgggaga
     1441 ttgctgttat ccgcgtcgaa tgttttttgt ggcccatcga cagtcgtcct tgcgaggaaa
     1501 cccctcaatc agtaccatat tggtacgtat tgagaggttc gcagcccgat gatccgtgtc
     1561 agccgtcttg ccgactacgc ctccgcggtg atggcctcgc tggcccggca ccctggcgag
     1621 gtgcttcctg cggcggcggt ggcggaggaa acccgcctcg aaatgccgac cgcgagcaag
     1681 ctgttgaagc ggctcgcccg tgcgggcctg gtggaatcgt ttcgtggcgc ggccggaggc
     1741 taccggctgg cgcgtgccgc cacggacatt tcgctcgcgg aaatcgtcga ggcgctggac
     1801 ggacccatcg gattgaccgc ctgccgcatc gggcgcaccg ggtgcgcgcg ccagccgtac
     1861 tgcaccgtct cgcatggctg gcaaggggtc ggcgccgcaa tcgacagcgc gctgcgcgcg
     1921 gtaagcctgg ccgacatggc acagcggcag ccggcacgcg tgcacggcgc tcgcatggca
     1981 tagggagtta cgcatggcaa cccaacccag cgaagtccag gccgcattgt cccgccgcta
     2041 tgaagcggga ttcgtcacgg acatcgaatc cctgagcctg ccgcccggcc tcgacgagga
     2101 cacgatccgc gcgctttcgg cgctcaagga ggagccggac tggatgaccg aatggcggct
     2161 gtccgcgtac cggcactggc tgacgatgaa gcagccggat tgggcgcacg tgaatctggc
     2221 gccgatcgac ttccaggcga tcagctacta ctcggcgccc aagaacaagc cgaagtcgct
     2281 ggacgaagtc gatccgaagc tgctggaaac ctatgacaag ttgggcgtgc cgctgcacga
     2341 gcgtgccaag ctggcgggcg tcgcggtgga cgcggtattc gattcggtgt cggtcgccaa
     2401 caccgcgcgc gagcagctcg cgaaggtcgg cgtgatcttc tgctctatgt cggaggcgat
     2461 ccgcgaatac ggtgacctgg tgcgccagta cctcggtacg gtggtgccgc agggcgacaa
     2521 cttcttcgcg gcgctgaact ccgcggtatt ctcggacggc tcgttcgtgt tcattccgaa
     2581 gggtgtgcgc tgcccgatgg aactgtccac ctacttccgc atcaacgcgg gccatacggg
     2641 gcagttcgaa cgcaccctga tcatcgccga agaggattcg gtggtgtcgt atctggaagg
     2701 ctgcaccgcg ccgatgcgcg acgacaacca gttgcacgcg gccgtcgtcg aactggtcgc
     2761 gctggaacgc gccagcatca agtactcgac ggtacagaac tggtaccccg gcgacgaaaa
     2821 tggcaagggg ggcatctaca acttcgtgac caagcgcggc gagtgccgcg gcgacgattc
     2881 gcggatcacc tggacccagg tcgaaaccgg ttcggcgatc acctggaaat acccgagctg
     2941 cgtgctgcgc ggcgatcgct cggtcggaga attccattcg gtggcgctga cccaccatta
     3001 ccagcaggcc gacaccggca ccaagatgat ccacatcggc cgcgacacca aatccaagat
     3061 cgtttcgaag ggcatcagcg ccggccgcag ccagaacagc tatcgcggcc tggtgaaggt
     3121 ggagaaggga gccgcgaacg cgcgcaacca cacccgctgc gactccatcc tcatcggcaa
     3181 gaaatgcggc tcgcacacct atccgtacgt cgaggtgaag aaccccacgg cgatcgtcga
     3241 gcacgaggca agcacgtcca agatttccga cgaccagttg ttctattgcc gctcgcgcgg
     3301 catcggcgaa gaagacgcgg tctcgatgat cgtcgacggc ttctgcaagc aggtgttccg
     3361 cgagctgccg atggagttcg cggtggaggc caagaagctg ctggaagttt cactggaagg
     3421 cgcgatcggg tgagcgtagc gcctcgtgca ggcgcaaagc ggcgcgaccc gctgggttcc
     3481 ctccccttca aggggagggt cagggtgggg atgggtttgc agttgacgtt tcaaccccat
     3541 ccccatccca gccttcccct tgaaggggaa ggagtcgaac acagaggatg acatgctgaa
     3601 aatcgaaaac ctccacgccc gtgtcgcagg caaggaaatc ctcaaggggc tctcgctgga
     3661 agtgaaggcg ggcgaggtgc acgcgctcat gggtccgaac ggagcgggca agtccaccct
     3721 cggaaacgtg ctgtccggcc ggcccgggta cgaggtcacc gaaggcatcg tcacgttcga
     3781 tggagcgaat ctgctcgaca tggcgccgga agaacgcgcg gccgcgggcg tgttcctggc
     3841 gttccagtac ccggtcgaaa tccccggcgt caacaacacc tatttcctgc gtgcggcctt
     3901 gaatgcgcaa cgcaagtcgc gtggcgagga ggaactcgac tccatgcagt tcctgaagct
     3961 ggtacgcggc aaggtcaagg agatgggcat gacccccgaa ctcctgaatc gtgcggtgaa
     4021 cgagggcttc agcggcggcg agaagaagcg caacgaggtg ttccagatgg cagtgctgca
     4081 accgcgcctc gccatcctcg acgagaccga ttcgggcctc gacatcgacg ccttgaagca
     4141 ggtcgccgag ggcgtcaacg cactgcgttc gccggggcgc gccttcctgg tgatcaccca
     4201 ttaccagcgg ctgctcgatt acatcgtgcc tgatttcgtg catgtgctcg acggcggacg
     4261 gatcgtcgag agcggcgacg cacggcttgc acaggacctc gaggcacatg gctacgcgtg
     4321 ggtgcgcgac cgcatgccgg ccggggcgca ggcgtgaaca ccgtgccggc accctttgtc
     4381 gaatcgatgc gggccgccgc ggccgccctg cggctgcccg gcagcggact cgcctggctc
     4441 gatgccgcgc ggtgcgaggc gctgcaggcc tttgtggagc gcggcttgcc caccgcccgc
     4501 agcgaactgt ggaagtacac cgcgttgcgg gcgctggcgc agcgccagta cgcggcaggt
     4561 gacggcgagg ctgcgggccg caccattgat gatgcgttgc tgccggcgca cgcgggcgcg
     4621 cgcgtggtgt tcgtcaacgg cgtgttccgc gccgatcttt cccgactgga tgttttgccc
     4681 gacggcgtcg tgctgcggtc gctggcggcg gcgctgcgcg agcagcccga accgctgcgt
     4741 ttcctgctgg ggccgcacaa ggacgacgcg gacgatggtt tcaccctgtt gaatcgtgcc
     4801 ctggcggccg acggggtgat cctgcgggtc gcgccgggcg tgcaggtggt cgaacccgtc
     4861 cacgtcgtgc acgtcggcgt tgccgcgcag caggcggtgg catgccactg tcgcagcctc
     4921 atcgaactcg gcgagggcgc gcgcctggga atcgtcgagc accacgtggg cgaagcggcg
     4981 gccggacacc tggtcaacct ggttcgcaag gtcaagctgc acgatcgggc gcggctcgac
     5041 tgggtgggta tccagtcttc gcccgccgat gcgacgctgt tgcgccgcag tgacgtggtg
     5101 ttgcacgacc gcgcccaact cgacttccat gcgctggaac tcggcgggaa gcttgcgcgc
     5161 cacgagctgc ggatcgacct cgcgggtgcc ggcgcgcgat tccgcagccg cggcgcgttc
     5221 gtattgcacg ggcgccagca cgccgacacc gaggtgctgg tgacgcaccg cggccgcgat
     5281 accgcgagcg actcgttgtg gcgcggcgtc gcggacgatc gggcccgcgg cgtcgtgcac
     5341 ggccggatcc tcgtgatgcc gggcgccgac ggcgcggacg gcagctttta caacaagaac
     5401 ctgctgcttt cggcgtctgc cgagatcgat acccgcccgg cgctggaaat ctacgccgac
     5461 gaggtcaagg ccaaccatgg cgcaaccgtg ggccagctcg atgaaaacgt gttgttctat
     5521 ctgcgctcgc gggggatccc gctggatgtc gcacggcgga tgctggtgca ggcgttctgc
     5581 gcggtgacgc tgcagggcat cgagcccgca ccgctgcggg agcgttgcga aacactgctg
     5641 gtcgagcagt tgccggcggt ggcggcgtga acgccaatcc ggaatcggcc gcggtgaccg
     5701 cgtccgtggt cgactggtcg cgggttcgcg aggatttccc gatccttgcg cgcaaggtgc
     5761 acggcaagcc cctggtgtat ttggacagcg ccaacacctc gcagaagccg cgccaggtca
     5821 tcgaggcggt ggatgcgttc taccgcgagc acaacgccaa cgtttcgcgg gccgtgcatc
     5881 agttgagcga ggaggcgacc gcgctgtacg aaggcgcgcg caccaagctc gcctcgttcc
     5941 tgggcgccaa ccaccgcgag gagatcgtgt tgacgtcggg caccacgatg gcgacaaacc
     6001 tggtcgcgta cagctacctg ttgccgcgcc tgaagcccgg cgacgcggtg ctcgtgacgg
     6061 cgatggaaca ccacgccgac atcgtgccgt ggcaactcgt gtgcaagctc acaggtgcgc
     6121 acctcaaggt cgcgccggtg aacgatgcgg gcgagctgat catcgggaag ttcatggaag
     6181 cactgacgcc cgaggtgaag ttcgcgggcg tggtccacgt ctccaatgtg ctcggcacca
     6241 tcaatccggt gaaggagctc gcgcgtgaat gtcgcaggcg cggcatcccc ctgttggtcg
     6301 atggatccca ggccgcgccg cacctgccgc tggacgtgaa atcgatcggc tgcgatttct
     6361 attgcgtgac cgggcacaag atgtgcggcc cgaccggcac tggcgcgctg tgggcccgcc
     6421 gcgagctgct cgaaacgatg ccgccgttct tcggcggcgg cgagatgatc aagaccgtct
     6481 ccttcgacgg caccactttc gcggactcgc cgcgcaagtt cgaggcggga acgccgaaca
     6541 cggcgggctt cgcgggactc ggtgccgcga tcgattatct ttccggcatc ggcatggaca
     6601 acgtcgccgc acgcgaacgg acactgctcg attatgcgac gccgcgcctc caggcggttc
     6661 ccggccttcg gatcttcggc acttcgccgc acaaggccgc ggtgatttcg ttcctgatcg
     6721 acggcgtgca cgcgcacgac ctcgccacgc ttttggacca ggaagggatt gccgtccgtt
     6781 ccggccacca ttgcgcgcat ccgctgatgc agttctacgg cgtggccgcc acctgccgcg
     6841 cgtcgctggc gttctacaac accgaatccg agatcgacgc cttcgtcgat gcgatcgggc
     6901 gggtgcggaa gttgctcgca tgaagaacgg ggaccgggga ccggggaccg ggggccggga
     6961 gccgcaccgt tcgggggcac atgcggccct ggtgcaccgc ccgctgtgtt ttcgctcagc
     7021 gacggaagcc gatgtcgagg cggtcgtcgc gctgacccag tcggcctatc gcggcgatca
     7081 aagtcgtgtc ggctggacca cggaggcgga cctcctcgat ggccagcgaa ccgacgcgcg
     7141 ggaggtcggg gagttgatcg cgaacccgga tgtgaaattg ctgctggccg agcgcgacgg
     7201 tcgactgctg gcgtgctgcg tcgtcgagcg atcggccaat gcacacccgg gggagggtta
     7261 tttcgggatg ttttcggtcc gtccggatgc gcagggcaac ggcacgggtc gtgcgctgct
     7321 cgccgaagcg gagcgggtcg cccgcgacga ctggcactgc cgggcgatgc ggatgacggt
     7381 gatcgactgc cgtgcggaat tgatcgcgtg gtacgaacgc cgcggttacc ggcgcaccgg
     7441 cgaacaccgc ccgttcccgt acggcgatcc ccggttcggg atcccgaagc gcgacgacct
     7501 tcgattcgaa tggctcgtca agaagttgtg aggagcgttt cgttttttgc ggagccgggt
     7561 cgcgtgcagg gcgcgctcct gcaactgcat gcccgcgacc agcgcatcgg aaaaatcatt
     7621 cggttcgcgc cggcgtgacg gagacagaag atgtcggaag agtgggtaaa ggtctgcgcg
     7681 caggacgaac tcctgccggg cgagttccgg gtggtgtggg acgaagacac ggcgatcgcc
     7741 gtgtacaacc tcgaaggcga cctgtatgcg atcgaggaca cctgcaccca tgatggcggc
     7801 gagctcgcgg gcggcgaggt gtgtggcttc gaggtcgaat gtccgcgcca tggggcgcgc
     7861 ttcgacgtgc gtaccggcga ggtgaagcag gcaccggcga ccgagccgat cgcgagcttc
     7921 ccggtgcgcg tcgaggaggg aatcatctac acacgggacg atcggtctta gtccgcccgt
     7981 cgaagcggct gttgtggaaa gccaggccag ggaagcccgt cgtggcgtcg gcgaaccagc
     8041 aatcagtccg cgtgaccttc gatttggcgg agcagcgatc ggggacgatc gcactaattt
     8101 gccggaaagc ggatttccgc gcgcagcccg tgcgggtcac gaagattgat gaaggaaagt
     8161 tcggcgccgt ggcgttcggc gatttcgcgc acgatggcga ggccgagccc ggtgccttcg
     8221 tggccattgc cgatggcgcg gaagaagcgt tcccccagcc gcggcaccag ttccggcgcc
     8281 acgccgggtc cgttgtcctg cacgctcagc acgcagccgc ggccgtcgtc gggctgcaag
     8341 ccgacggtga tcgcgggatc gccgcgggtg ccgtagcgca tcgcgttgtc gatcaggttg
     8401 ccgagcattt cgcgcagcag gacggcgtcc ccgatgatcg tgacgccgcg atcggggccc
     8461 tggtagccga ggtcgatgcc gcgcgcgatg gcctccggca cacgtgacgc cacctcgtcc
     8521 cgcaccacgt tcgcgaggtc gagcggcgtc gcgacgccca gcgactcgtc cggcgcctgg
     8581 gcgcgcgcca gcgccagcag ttggccggcg gcgcgggcgg tgccggcgga gagccgttcg
     8641 acgtgggtca gggcttcgcg gatggtgtcc gggcgcggat ccgcaagcgc gcgttccgcc
     8701 tgcaggcgca agccggcgag cggcgtccgc agctggtggg cggcatccgc gatgaagcgc
     8761 tgctgctgcc gcagcatccg gtcgaggcgg ctgagcagcg catcgatggt cgcgaccagc
     8821 ggttgcactt cgatcggcgc gtcgatcgtt cgcagtggtg aaagatcgcc ggcatcgcgc
     8881 atccggattt catgcgccag tggatccagc acgcgcaagc ctcggttgat gccgagccac
     8941 accagcgcca tcgcgacgca gatcagcagg agttcgatgg gggccgtcga aaacaggagt
     9001 tcccgcgcca acgccttgcg gttgcgcacg gtttcggcgg tggagatcac caacgtgtcg
     9061 cccgggtcgg ccttgttcgg gaggaacacc gacgccatgc gcatggcctg tccgtggtcg
     9121 gaaaagttgg tgaagaccga gccgccgccg gacccgtcgg gcatgggctg cggcaggtcc
     9181 tggtcgcccg ccagcacgcc atagcgcagg ctgcggaccg cgaagatgtt gcggtcgtgc
     9241 accgagaact gcaggaggat ccgggcctgc tcgctgagtt cgttgcggcc ggcgtcgctg
     9301 ttgacgaggt cgcccatggc gttcgcgctg ttcagcagcc agcggtcgta gaccttgttg
     9361 gcgtagtgca gcccgatcac gtagttgatg atgccgccgc agatcagcac cgccagcagc
     9421 ggcaccgaaa cgaacatcag caggcgccgg cgcaggctgg ggttacgaac catcgtcggc
     9481 ctccagcagg tagccgagtc cgcgcacggt tcgcagcgtg acgtcgctct ccgccagctt
     9541 gcgccgcagg cggtgcacgg cgatgtcgag cccgttgtcg gtgagcgcgt cgttccaggt
     9601 gcacagcgcc tcgatcacgt gttcgcgcgg cgtgacccgg ttgcggcgcg aggcgaggac
     9661 ctcgagcagc gcgaattcac gcgccgtcag ctccagcggc tggtcaccct gcagcacccg
     9721 gcggccggcg aggtcgatcg tgagcttgcc gaggtgcagg accggcatcc cgcggttgcc
     9781 ctggcggcgc aacaacgcac gtacccgggc atcgaattcg gcgagactga agggcttgac
     9841 caggtaatcg tcggcgccga ggtccaaggt gcgtacgcgc aggtcgactt cctcgcgcgc
     9901 ggtgatcacg aggaccggca cgccgtcgtg cgacgtgcgc agccgccgca ccacttcgct
     9961 gccgtcctgt cccggcaagc ccaggtcgag tacgaccagc ccgaacgcgt gttcctggag
    10021 cgccgcgatc gcggctttcc cgttggacag cctgtcgacg gcgtggcccg cccgctccag
    10081 cgaatcgcaa accgcgtcgg cgatggccgc atcgtcttcg actaccagca tccgcatttc
    10141 gggcccgccg gtgggtggga aggcatcaag cgtacaactt gacgagcacg atttcgcccg
    10201 cgccctcgac cagcacccgc tcgggggcgg cgccggtcgc gaccagcacg gcctcgcgcg
    10261 gttccagcgc gaggcttccg ccgccgccgg catcgacccg tgagcggccg ctgtcgaggt
    10321 ataccagcca gcgcaccccg ggcagcgagg gcagcaccat cgaatcgacc agcgtgcggg
    10381 gcagcacgtc gccgcgggcg ccgccgcgca gcatcaggtt gaagtctcgc gtcgggcctt
    10441 ccggcagcag accggcgggg gcaggcgatc cgtcgaaacg aagggtgcgg aagcgcggcg
    10501 cagccaggtc gcggccgtcg gggaagcgca gtcccatcgg cgcgtccagg ggaaccagta
    10561 cccgccgcgt atccggcagt tccgagaatg gtccgggcgc atccacgttg gcgatgctga
    10621 tgcggaccgt ccactcggca gcgtccggtt cccgcaacaa cacggtggtg ctcccggcgc
    10681 cattggccca tggctcctcg cgttgcgcgg aacggggtat ggcgcgcagg atcgacacct
    10741 cgccgatggt aacggcgccg cccttgcgtc agtcgagcag cttctgcagc gattcgatgc
    10801 cgagacgcag gctgtcgcgc cacggatacc ccacgatcgc ctcgccggtg tgcgcatcgc
    10861 gaaagctttc ttcctggcca ggcgccagca ggtggcggta atcgaccgtc aattcctcgc
    10921 ctggctcgag gtcccgggcg gcgaatatga accccagcac ccacaacccg ctcggctcga
    10981 aactgtggtt gacgaagcag tcgtccggcc aatccgggga cagcgtatag cggtcctcga
    11041 accagcgcac cgaggtgtgc atcagggccg cgcgttccgg gtcggccatg atctccgcga
    11101 acgaccaggt cccgtcgatc cggtccggcg cgatcgcgat gcgtccgtgc cggagcggct
    11161 cgtccaggaa cacgccctgg ccggcgccgg gaatcaggga aggtgcgatg tggtagcggg
    11221 gcaggatcac ggacagcgaa ctcgcgccag ggaaagcgcg cgagtgtagt gcgggcggtg
    11281 gggggggggg gggg
//
LOCUS       DASSNH010000035        10377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1105751, whole genome shotgun sequence.
ACCESSION   DASSNH010000035 DASSNH010000000
VERSION     DASSNH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105751"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFK00_01225"
     CDS             complement(<1..258)
                     /locus_tag="VFK00_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HET7591610.1"
                     /translation="MGAFTQWFNERTPGLGAVYRKHMSEYYAPKNFNVMYYFGSLALL
                     VLVNQIVTGIFLTMFYTPTAAGAFDSVQYIMRDVNWGWLVRY"
     gene            complement(258..854)
                     /gene="petA"
                     /locus_tag="VFK00_01230"
     CDS             complement(258..854)
                     /gene="petA"
                     /locus_tag="VFK00_01230"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7591611.1"
                     /translation="MADESINKGRRRFLTASTAVIGGAGLIITAIPFVETWLPSARAE
                     AAGSPITVDISKVEKGQMLRVGWRKQPIFVINRSPEQLKWLPKQDQLLRDPKSNESQQ
                     PEYCKNEHRSIKPEWMVLIGICTHLGCVPDYKGKVKPEPFDPDWKGGFYCPCHHSRYD
                     IAARVYQGVPAPLNMVVPPYHFVDDTHIQIGVDPKGAA"
     gene            1044..2219
                     /locus_tag="VFK00_01235"
     CDS             1044..2219
                     /locus_tag="VFK00_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7591612.1"
                     /translation="MLRKLRPLLFVLQFVVVGLAAAFVIAMLWPGAGDRLRAGFGTRP
                     SSHARTEAPAAHAPTGPASYADAVARATPAVVNIYANKIVTERPVQIFRDPLLRQFLG
                     GVAGPAMRRRESSLGSGVIFTADGYVLTNNHVISGADDIQVMLHDGRVAHARVIGTDA
                     DTDLAVLKINASQLPTIELDPEPPQVGDVVLAIGNPFGIGQTVTMGIVSALQRQLSLS
                     PYEDFIQTDAAINSGNSGGALVNAEGQLVGINTAMLDRDVGAQGIGFAIPVRTAKRVL
                     EQIVEHGRVVRGWLGLEVGNVVVSADSGLPGAARGATVLDVYPGGPAAQAGLQPGDIL
                     LKMGDQPIIDPGDLRSREAETPPGSKVKISGLRTGEPFTAEVVLAQRPAFKASDQQG"
     gene            complement(2194..3003)
                     /locus_tag="VFK00_01240"
     CDS             complement(2194..3003)
                     /locus_tag="VFK00_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7591613.1"
                     /translation="MTDTVFMVHGMWGAAFYWANFRGVLEACGWRCVATTLPFHDMAP
                     GTTPDPRLGRTGLLDYVAALERELDALGEPPVIIGHSMGGLLTQMLCARGRARAAVLL
                     APAAPAGILGLTPSVLKSFLPVMLRKGFWRKPVPPLRTAAYSMLHLLPAGEQRKVQAE
                     LVYESGRVAAEVGLWPLDRRHASRVDPAAVTCPLLVLAGREDRVTPVRVVRAIARRYG
                     ARAEYRELENHAHWLVGEPGWEAIAHGIATWLEPFRRAAPGADISPAGRMP"
     gene            complement(3230..3772)
                     /locus_tag="VFK00_01245"
     CDS             complement(3230..3772)
                     /locus_tag="VFK00_01245"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7591614.1"
                     /translation="MGESESALLERLKARVRDVPDFPSPGVLFKDITPLLADARAYGD
                     CVAAMAAPWREAALDAVCGIEARGFIFGAALAQALGVGFVPLRKAGKLPAATRGIDFG
                     LEYGEARLEIHADGLAAGAHALIVDDVLATGGTLAAAAGLVQSLGATIAGAAVVIELE
                     FLRGRERWCADAPLRSLLRY"
     gene            complement(3787..4068)
                     /locus_tag="VFK00_01250"
     CDS             complement(3787..4068)
                     /locus_tag="VFK00_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET7591615.1"
                     /translation="MRVVYHAENIVDAHLVKDALEQAGIPAFVNGEYLIGGVGQLPAR
                     DFLGVSVPEICIQAAEPVVRQMVEMLRAPIAEDREPDPGPLPSLRPAAT"
     gene            4265..5998
                     /locus_tag="VFK00_01255"
     CDS             4265..5998
                     /locus_tag="VFK00_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HET7591616.1"
                     /translation="MANEPSIVPTNFIRQIVLADQASGKHGGRVATRFPPEPNGHLHL
                     GHAKAICLDFGIAREFGGTCNLRLDDTNPGREDPAFVEGIKDSVRWLGFEWSALRHAS
                     DYFEVLYRAALKLIEDGKAYVDDLNADEVRAYRGTLTEPGRDSPHRNRAVEENLDLFR
                     RMRSGEFDDGAKTLRAKIDMAAGNINLRDPALYRIRKLVHQNTGDAWPIYPMYDFAHS
                     LSDALEGITHSFCTLEFEDHRPLYDWCVDNVDFANHPELWQPLVEKGLPTAPSKPRQI
                     EFARGNLSYTVMSKRKLAELVDKKLVDGWDDPRMPTLAGVRRRGFTAAAIRMFWERAG
                     LSKQNSVIDCGVLENSVREDLDAHAPRRMAVLDPLKLVITNLPEDHAETLMLANHPKD
                     ESFGAREVPFSRELWIERDDFAEVPPKGFKRLVPGGEVRLRGVGIVRCAEVVKDAAGN
                     AEELRCTLDPESRHGMEGANRKVKGTIHWVGASHAVEGEVRLYDRLFDVADPDDDRDG
                     RTWLEHLNPESRRSITARLEPSLREAQPEDAFQFERTGYFVADRHDHAPGRPVFNRAV
                     TLRDTWTKQSA"
     gene            6197..6541
                     /locus_tag="VFK00_01260"
     CDS             6197..6541
                     /locus_tag="VFK00_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7591617.1"
                     /translation="MKARNFAVIALAAALGFGMSAPAALAAQPGAAASDQSSSAGEAV
                     SDAWITTKVKSELATTKGVKSMDVSVDTKDGVVTLTGVLATDVDVKKAIAATKSVKGV
                     KEVDASGLKAKD"
     gene            6547..6792
                     /locus_tag="VFK00_01265"
     CDS             6547..6792
                     /locus_tag="VFK00_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7591618.1"
                     /translation="MTTLPPLNRAHATSAILGHDRIKEEWPTIAGRIRRRWRKLTSDD
                     VGFPGGSAEYLARVLQDRYGVDRREALLQVYEFESEL"
     gene            6847..7044
                     /locus_tag="VFK00_01270"
     CDS             6847..7044
                     /locus_tag="VFK00_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7591619.1"
                     /translation="MNEDKIKGQWKQLSGKIKARWGKLTDDDLTAASGNAEYLAGKVQ
                     ERYGIAKDEAHKQVREFESDL"
     gene            7130..7291
                     /locus_tag="VFK00_01275"
     CDS             7130..7291
                     /locus_tag="VFK00_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET7591620.1"
                     /translation="MLYYAIVFLIIALIAGFFGFFGVAGLAAGIAKILFVIFIILFIV
                     ALIFGRRRV"
     gene            7474..8046
                     /locus_tag="VFK00_01280"
     CDS             7474..8046
                     /locus_tag="VFK00_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7591621.1"
                     /translation="MRPHAPSKLPVPTLPAWIRGAIDFDRTYANRKARVAVAIALARG
                     NVVHATGGPFGAAVFDDRGALIAVGVNRVLPLRSSLAHAEVIALAGAQQKNGRARLNE
                     DGRRYTLATSSQPCCQCYGAIVWAGIDELLVGARAEDTESLAGFDEGPLPADWVGELE
                     RRGIRVERDLDRDAAREVLAAYARRGGPRY"
     gene            8183..8863
                     /locus_tag="VFK00_01285"
     CDS             8183..8863
                     /locus_tag="VFK00_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7591622.1"
                     /translation="MPLLILVAIGVAVGASGMLEHLSPHRIMAEQANWSQQIASRPAL
                     AYIIYIAVLSLSIATAMPGPLLIIIAGGMLFGMGKAIGLSLAGEVLGSLLLFFAARHA
                     FGTGRRPPPKFVERIRQGYQLHPVSYTLFLRFVPLFPFGGITVALAWLRCPVWLFTSA
                     TALGGVIMLVFETAIGAGLSQSIAEGKGITPHLILEPHIWLPLVGLGLLALIPVLIQK
                     LRSNGSTE"
     gene            9043..>10377
                     /locus_tag="VFK00_01290"
     CDS             9043..>10377
                     /locus_tag="VFK00_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017160292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HET7591623.1"
                     /translation="MTTTAATAASAPEASAAPLPRQIPYIIATEGCERFSFYGMRNIL
                     TPFLVSTLLLYLPLEERAGAARDVFHTFVIGVYFFPLLGGWIADRLWGKYHTVLWLSM
                     VYVAGHACLAAFEGNRPGFYTGLFLIALGSGGIKPLVSAFVGDQFDASNKHRAKLVYD
                     LFYWIINFGSFFASLLMPLFLRGFGPAVAFGIPGALMAIALCAFWAGRKRYVRIPPAP
                     PNPDSLLRVVRTALLSRTGGAARPGLWVAAAGLAFGLAALAAIPWLGFVIGACLALGC
                     LLAFGGCGTAMQLERARGVHPDMAVDGVRGVLRLMIVFALVTPFWSLFDQKASTWVLQ
                     GAQMVKPGWFHEAQMQALNPMLVLLLIPFNNLVLFPVMRRIGIEPTALRRMGAGIAFA
                     ALAWTAAGFIQLEIDGGDPVSIAWQVLPYALLTLGEVLVSATGLEFAYSQAPP"
BASE COUNT         1728 a         3531 c         3372 g         1746 t
ORIGIN      
        1 gtagcggacc agccagcccc agttgacgtc gcgcatgatg tactgcaccg agtcgaacgc
       61 gccggcggcg gtcggggtgt agaacatcgt caggaagatg cccgtgacga tctggttcac
      121 cagcaccagc agcgccagcg agccgaagta gtacatcacg ttgaagttct tcggagcgta
      181 gtactccgac atgtgcttcc tgtagaccgc accgagcccc ggggtacgct cgttgaacca
      241 ttgcgtgaac gcgcccatta cgcggctcct ttcggatcga cgccgatctg gatgtgggtg
      301 tcatcgacga agtggtatgg cgggaccacc atgttcagcg gcgccggcac gccctggtag
      361 acgcgcgcgg cgatgtcgta gcgcgagtgg tggcaggggc agtagaagcc gcccttccag
      421 tcgggatcga acggctcggg cttcaccttg cccttgtagt cgggcacgca gccgaggtgc
      481 gtgcagatgc cgatcagcac catccactcg ggcttgatgg aacggtgctc gttcttgcag
      541 tactcgggct gctgcgactc gttggacttc ggatcgcgca ggagctggtc ctgcttcggc
      601 agccatttga gctgctccgg cgagcggttg atcacgaaga tcggttgctt gcgccagccg
      661 acccgcagca tttggccctt ctcgaccttg ctgatgtcga cggtgatggg cgagccggcc
      721 gcctcggcgc gtgcgctcgg cagccaggtt tccacgaacg ggatcgccgt gatgatgagg
      781 cccgcgccgc cgatcacggc ggtggaggcc gtcaggaatc tgcgccgccc cttgttgatg
      841 ctttcgtccg ccatcagctt ctccgcgcta cctcgggtcg cggcgaccga ccgccgccgt
      901 gactgcatgc cccacttggc cagaccggga tgaacggctc tgtccctgcc gcgaccgggt
      961 catccatcgg gccccaaaat cgtgttagtt tagcgttcgc ctcttgagcg cacaatccga
     1021 cggcgcccga ggggcgcatc tccatgcttc gcaagctccg tccgcttctt ttcgtcctgc
     1081 agttcgtggt ggtcggcctt gcggctgcat tcgtgatcgc gatgctgtgg ccgggcgcgg
     1141 gcgatcgcct gcgcgcgggg ttcggcaccc gcccatcgag ccacgcgcgg acggaagcgc
     1201 ctgcggcgca cgcgccgacc ggccctgcct cgtacgccga cgccgtggcc cgcgccacgc
     1261 cggccgtcgt gaacatctac gccaacaaga tcgtcaccga gcgccccgtc cagatcttcc
     1321 gcgacccgtt gctgcgccag ttcctcggcg gggtggccgg gcccgcgatg cggcggcgcg
     1381 aatcgagcct cggctccggg gtgatcttca ccgccgacgg ctacgtcctc accaacaacc
     1441 acgtgatctc gggggccgac gacatccagg taatgctgca cgacgggcgg gtggcacatg
     1501 cacgcgtgat cggcaccgac gcggacaccg atctcgcggt actcaagatc aatgccagcc
     1561 agcttccgac gatcgaactc gacccggagc cgccgcaggt cggcgacgtg gtgctggcga
     1621 tcggcaatcc cttcgggatc ggccagaccg tcaccatggg catcgtcagc gccctgcagc
     1681 ggcagttgag cctgtcgccg tacgaggact tcatccagac cgacgcggcg atcaactccg
     1741 gcaattccgg cggcgcgctg gtgaacgccg agggccagct ggtcggcatc aacaccgcaa
     1801 tgctggaccg cgacgtgggc gcgcagggaa tcggcttcgc gataccggtg cgcaccgcca
     1861 agcgcgtgct cgagcagatc gtcgagcatg gacgcgtggt gcgcggatgg ctgggcctcg
     1921 aagtcggcaa tgtcgtggta tccgccgatt ccggattgcc gggcgcggcg cgcggcgcca
     1981 cggtgctcga tgtctatccg ggcggccccg ctgcgcaagc gggcctgcag ccgggcgaca
     2041 tcctgctgaa gatgggcgac cagccgatca tcgatcccgg cgacctgcgc agccgcgaag
     2101 cggagacgcc gcccggcagc aaggtgaaga tctccgggtt gcgcacgggc gagccgttca
     2161 cggccgaggt ggtgctcgcc caacggccgg ccttcaaggc atccgaccag cagggctgat
     2221 gtccgcgccg ggcgcggcgc gccggaacgg ttccagccac gtggcgatcc catgggcgat
     2281 ggcttcccac cccggttcgc ccaccagcca gtgggcatgg ttttcgagtt ctcggtattc
     2341 cgcgcgtgcc ccgtaacggc gcgcgatggc gcgcaccacc cgcaccgggg tgacgcggtc
     2401 ctcgcggcct gcgagcacca gcagcgggca ggtcaccgcc gcgggatcca cgcggctggc
     2461 gtggcggcga tccagtggcc agagtcccac ctccgcggcc acccggccgg actcgtacac
     2521 gagctcggcc tgcaccttgc gctgttcccc ggccggcaac aggtgcagca tcgaatacgc
     2581 cgcggtccgc aacggcggaa ccggcttgcg ccagaagccc ttccgcagca tcaccggcag
     2641 gaagcttttc agcaccgacg gggtcaaccc caggatgccc gcgggcgcgg cgggcgccag
     2701 tagcaccgcg gcacgggccc ggccgcgcgc gcacaacatc tgcgtgagca aaccacccat
     2761 cgagtgtccg atgatgactg gcggctcacc gagcgcgtcg agttcgcgct cgagcgcggc
     2821 gacatagtcg agcaggcccg tgcggccgag tcgcgggtcc ggcgttgtgc ccggcgccat
     2881 gtcgtggaac ggcagcgtgg tcgccacgca gcgccagccg catgcctcga gcacgccgcg
     2941 gaaattggcc cagtaaaagg ccgcgcccca catgccgtgg accatgaaca ccgtgtcggt
     3001 catcacccgc tccatgcatt gccccatgat taccataccg agacatccgg actgtcgcga
     3061 ccgccgacgg gcgacgcgac caaaatgact gttggttggg gacgacggtg ctaggcgccg
     3121 acgatgcgat ccagggcacg gcgaacccct cggcagtgcg gcggcgccgc ggcccgcgca
     3181 ttcgccggcc gaattgcccg gttaccgaga aatgcaggcg caccagcgat caatagcgca
     3241 gcagcgagcg aagcggtgcg tcggcgcacc agcgctcgcg gccccgcagg aattccagtt
     3301 cgatcaccac cgcagcgccc gcgattgtcg ctccgaggct ttgcaccagc cctgccgccg
     3361 cggcgagcgt accaccggtg gccagcacgt catccacgat cagcgcgtgc gcgccggccg
     3421 ccaagccatc ggcatggatt tcgagccgcg cctctccgta ttccaatccg aaatcgatcc
     3481 cgcgggtcgc ggccggaagc ttgccggcct tgcgcagcgg cacgaaaccc acgccgagcg
     3541 cctgcgccag cgccgcaccg aaaatgaagc cgcgtgcctc gatgccgcac accgcatcga
     3601 gcgccgcttc ccgccacggc gccgccatcg cggccacgca atcgccgtac gcgcgggcgt
     3661 cggcgagcag cggcgtgatg tccttgaaaa gcacgccggg gctcgggaaa tcggggacgt
     3721 cgcgaacgcg tgccttcaaa cgctccagca gcgcggactc cgattccccc attgcaaccg
     3781 tggcgttcaa gtggctgcgg gccgcagcga tggcagtggc ccggggtcgg gctcgcgatc
     3841 ctccgcgatc ggagcgcgca gcatctccac catttggcgc accaccggct ccgccgcctg
     3901 gatgcagatc tccggcacgc tgactcccag gaaatcgcgc gcgggcaatt ggcccacgcc
     3961 cccgatcagg tactcgccgt tgacgaaggc ggggatcccc gcctgttcca gcgcgtcctt
     4021 gacgagatgc gcatcgacga tgttttccgc gtggtagacc actcgcatgg cgtcaaacta
     4081 atgccgttca tcatggttga caagcgcggg ggcccactgg gcttttccaa gcaaagcatt
     4141 ccacgcggat cgaggcggat tcacgcggat ggcagtttcc gatccggaat cagccgcgca
     4201 aatccgcgtc agaaagtgct tcttccacgt taaactgctc ggcttgacga atcccggatc
     4261 atccatggcg aacgagcctt ccatcgtccc gaccaacttc atccgccaga tcgtgctcgc
     4321 cgaccaggca tccggcaagc acggcggacg ggtcgcaacc cgtttcccgc ccgagcccaa
     4381 cggccacctg cacctcggcc acgccaaggc gatctgcctg gatttcggca tcgcgcgcga
     4441 attcggcggc acctgcaacc tgcgactcga cgataccaac ccgggccgcg aagacccggc
     4501 gttcgtcgaa ggcatcaagg acagcgtgcg ttggctcggg ttcgaatgga gcgcgctccg
     4561 ccacgcctcg gactacttcg aggtgctcta ccgcgccgca ttgaaattga tcgaggacgg
     4621 caaggcctac gtggacgact tgaacgccga cgaggtgcgg gcgtaccgcg gcacgctgac
     4681 cgaacccggc cgcgattcgc cgcaccgcaa tcgtgcggtc gaagaaaacc tcgacctctt
     4741 ccggcggatg cgttccggcg agttcgacga tggcgcgaaa acgctgcgcg cgaagatcga
     4801 catggccgcg ggcaacatta acctgcgcga tccggcgctg taccggatcc gcaagctcgt
     4861 ccaccagaac accggcgacg cctggccgat ctatccgatg tacgactttg cgcattcgct
     4921 ttccgatgcg ctcgaaggca tcacgcattc cttctgcacg ctcgagttcg aggaccaccg
     4981 gccgctgtac gactggtgcg tcgataacgt ggacttcgcg aaccatccgg aactgtggca
     5041 gccgctggtc gagaagggcc tcccgaccgc gccttcgaag ccgcgccaga tcgagttcgc
     5101 ccgcgggaac ctttcctaca ccgtgatgag caagcgcaag ctcgccgagc tcgtcgacaa
     5161 aaagctcgtc gacggctggg acgacccccg gatgccgacc ctggccggcg tgcgccggcg
     5221 cggcttcact gcggcggcga tccgcatgtt ctgggagcgg gcgggactct ccaagcagaa
     5281 cagcgtcatc gactgcggcg tgcttgagaa cagcgttcgc gaagacctcg acgcgcacgc
     5341 tccccgcagg atggcggtgc tcgatccact gaagctcgtg atcaccaacc tgccggagga
     5401 ccacgccgag accctcatgc ttgcgaacca ccccaaggac gaatcgttcg gcgcgcggga
     5461 ggtgccgttc tcgcgcgagc tgtggatcga gcgggacgat ttcgccgagg ttcctccgaa
     5521 gggattcaag cgcctcgttc ccggcggcga agtgcgcttg cgcggcgtcg gcatcgtgcg
     5581 ctgcgcggaa gtggtcaagg acgccgccgg caacgccgag gaactgcgct gcacgctcga
     5641 tcccgaatcg cgccacggca tggaaggcgc caaccgcaag gtcaagggca ccatccattg
     5701 ggtcggcgca agtcatgccg tggaaggcga ggtgcgtctg tacgaccggc tgttcgacgt
     5761 ggccgatccg gatgacgatc gcgatggccg gacttggctg gaacacttga acccggagtc
     5821 gcgccgcagc atcaccgcga ggctcgagcc gtccctgcgc gaggcgcagc cggaggacgc
     5881 attccagttc gaacgcaccg gctatttcgt ggccgatcgc cacgaccatg cgcccggtcg
     5941 accggtattc aaccgcgcgg tcacgttgcg cgatacctgg acgaagcaat ccgcgtagcg
     6001 ggcatttcct gccgccggac cgccggcggg gcggcatcgt cgatcacgcc gacgaacccc
     6061 gtaaccgctt gatccggcgg ttgcgcgcgt tcagtttcgg ttcaaaagtc atagccctcg
     6121 tcgttcatat ccgcgctgat acgttgagcg cgcatcgccg ggagtggcga tgcaccacac
     6181 aacaaggaga tcaacgatga aggcacgcaa cttcgcggtc atcgccctcg ctgcagcgct
     6241 cggattcggg atgtccgccc ccgcggcgct cgccgcgcaa ccaggggccg cggccagcga
     6301 tcagtcctcg tccgcaggtg aagccgtcag cgacgcatgg atcacgacca aggtgaaaag
     6361 cgagctcgcc accaccaagg gcgtgaagag catggatgtt tcggtggata ccaaggacgg
     6421 cgtggtgacc ctgactggcg tcctggctac cgacgtcgac gtgaagaagg ccatcgccgc
     6481 caccaaaagc gtcaagggcg tcaaggaagt ggatgcttcc ggcctgaagg caaaggactg
     6541 accgacatga caacccttcc cccactcaac cgcgcccatg cgacgtccgc gatcctcggc
     6601 cacgaccgca tcaaggaaga atggcccacc attgccggaa ggattcgccg ccgctggcgc
     6661 aagttgacca gcgatgacgt gggttttccg gggggcagcg ccgaatacct cgcccgcgtc
     6721 ctgcaggacc gttacggcgt ggaccggcgc gaagccttgc tgcaggtcta cgaattcgaa
     6781 agcgaactgt aatccgacgc cacccaggtg gcccggtcgc cgcaccagcc cgacatggag
     6841 aacacgatga acgaggacaa gatcaagggt cagtggaagc aactgagcgg caagatcaag
     6901 gcgcgttggg gcaagctcac cgacgacgac ctcacggccg cttccggcaa cgcggaatac
     6961 ctcgccggca aggtgcagga acgctatggg atcgccaagg atgaagccca caagcaggtc
     7021 cgggagttcg aaagcgacct gtagtcgaag acggaaacga ccaccaagcg cgggtcatca
     7081 acgtgtcctg tgaaccgaac gcaatgcatc gtcgcacaag gagagcgcca tgctgtatta
     7141 cgccatcgtc ttcctcataa tcgcgttgat cgccggattc ttcgggttct tcggggtcgc
     7201 cggcctcgcc gccgggatcg ccaagatcct gttcgtgatc ttcatcatcc tgttcatcgt
     7261 ggcgctgatc ttcgggcgcc gaagggtgtg aggcagcacg atccggcagt cgctcggggc
     7321 attgcgcggc agtaaagccc tgggcggagc caccctgtcc gtcacaagcc gagcccggtc
     7381 gcccgggctc ggctttcgca tgccgcgcgt tcgcctggct tgcctggtcg gacgcatgcc
     7441 cccccttcgc ggcacaggtg ctacgttgcg accatgcgac cgcacgcccc atcgaagctt
     7501 cccgtcccca ccctgccggc ttggatccgc ggggccatcg atttcgatcg cacgtatgca
     7561 aaccgcaagg cacgcgtcgc cgtcgcgatc gcgctggcac gcggcaacgt ggtacacgca
     7621 accggcggac cgttcggcgc tgcggtattc gatgaccgcg gcgccctgat cgcagtcggg
     7681 gtgaaccgcg tcctgccgtt gcgatcatcg ctcgcccacg cggaagtcat cgcgcttgcc
     7741 ggcgcgcagc aaaaaaatgg ccgcgcccgc ctcaacgagg atggacggcg ctacaccctg
     7801 gccaccagtt cgcaaccctg ttgccagtgc tacggcgcga tcgtctgggc gggaatcgat
     7861 gaactgctgg tcggtgcacg cgcggaggac accgaatcac tggcgggatt cgacgagggg
     7921 ccgctgcccg ccgactgggt cggcgaactc gagcggcgcg gcatccgggt cgagcgcgac
     7981 ctcgatcgcg acgccgctcg cgaggtgctg gcggcatacg cgcggcgcgg cggcccccgc
     8041 tactgacggg ttggccttgg gcaacgtggg ccggaaatgg cgcggatacg caccccggtt
     8101 cagagcccga tcaaggccga ccggctattc ttttggattg cccttgtctg cctggttgcc
     8161 catgagccgg ttgcgagcgt tcgtgccatt gctgattctg gttgcgatcg gggtggcggt
     8221 cggtgcgagc ggcatgctgg agcatctcag tccgcaccgg atcatggcgg agcaggcgaa
     8281 ctggtcgcaa cagatcgcct cgcggccggc gctcgcctac atcatctaca tcgccgtcct
     8341 ctcgctgtcg atcgccaccg cgatgcccgg cccgttgctg atcatcattg ccggcggaat
     8401 gctgttcggc atgggcaagg ccatcgggtt gtcgctggcg ggcgaagtac tcggctcgct
     8461 gctgctgttc tttgccgcgc ggcacgcctt cgggaccggc cggcggccgc cgccgaagtt
     8521 cgtcgagcgc atacgccagg gctaccagct ccatccggtt tcctacacgc tgttcctgcg
     8581 cttcgtgccg ctgttcccgt tcggcggcat cacggtcgcc ctggcgtggc tccgctgccc
     8641 ggtctggctg ttcacgtccg cgaccgcgct gggcggggtg atcatgctgg tcttcgaaac
     8701 agccatcggc gcggggctga gccaatcgat cgcggaaggc aagggcatta cgccgcacct
     8761 gatcctggaa ccccacatct ggctgccact ggtgggcctt ggtctgctcg ccctgattcc
     8821 cgtgctcatc cagaagctgc gctcgaacgg ctcgaccgag tgaatcattg ccggagagct
     8881 tttgacgcgg atcaacgcgg acaaaaaaga gtcaaattgc ctcttgatcc gcgttgggtg
     8941 cgctctttcc caccccggca acgtgcaatg tgacgcgatc ctggtgattc ccgcccctgg
     9001 aggcgttacc ctcggctgcg aacacacgag cacgccctga cgatgacgac gaccgccgcc
     9061 accgccgctt ccgcgcccga agcttccgcc gcgccactgc cgcggcaaat cccctacatc
     9121 atcgccaccg aaggctgcga gcgcttcagc ttctacggga tgcgcaacat cctgacgccg
     9181 ttcctggtgt cgacgctgct gctgtacctg ccgctcgagg aacgcgcggg cgccgcgcgg
     9241 gacgtgttcc acacgttcgt gatcggcgtg tatttcttcc cgctgctcgg cggctggatc
     9301 gccgaccggt tgtggggcaa gtaccacacc gtgctgtggc tttcgatggt gtatgtcgcc
     9361 gggcacgcct gccttgcggc gttcgagggg aaccggccgg gcttctacac gggcctgttc
     9421 ctgatcgcgc tcgggtcggg cggcatcaag ccgctggtgt cggcgttcgt cggcgaccag
     9481 ttcgacgcca gcaacaagca tcgcgcgaaa ctggtgtatg acctgttcta ctggatcatc
     9541 aatttcgggt ccttcttcgc gtcgctgctg atgccgctgt tcctgcgcgg cttcggaccg
     9601 gcggtggcgt tcggcatccc gggtgcgctg atggcgatcg cgttgtgcgc gttctgggcc
     9661 ggccgcaaac gctacgtgcg gattccgccg gcgccgccga atcccgattc gttgctgcgg
     9721 gtggttcgca ccgccttgct gtctcgtacc ggaggcgcgg cgcggccggg attgtgggtc
     9781 gcggccgccg gtctcgcctt cggtttggcc gcgctcgcgg cgatcccgtg gctcggcttc
     9841 gtgatcggcg cctgcctcgc gctcggatgc ctgctggcgt tcggcggatg tggcaccgca
     9901 atgcagctcg aacgtgcccg cggcgtccat ccggacatgg ccgtggacgg ggtgcgcgga
     9961 gtcctgcgat tgatgatcgt gttcgccctg gtcacgccgt tctggtcatt gttcgaccag
    10021 aaggcttcga cctgggtgct gcagggcgcg caaatggtga agcccggctg gttccacgaa
    10081 gcgcaaatgc aggcgcttaa cccgatgctc gtgctgctgc tgattccgtt caacaacctc
    10141 gtgctgttcc cggtgatgcg ccgcattggc atcgaaccga ccgcattgcg gcgaatggga
    10201 gcgggcatcg cgttcgccgc gctggcatgg accgcggccg gcttcatcca gctcgagatc
    10261 gacggcggtg atccggtatc gatcgcctgg caggtgctgc cctatgcctt gctcacgctc
    10321 ggcgaagtcc tggtttccgc gaccggcctg gaattcgcct acagccaggc accccct
//
LOCUS       DASSNH010000036         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1381778, whole genome shotgun sequence.
ACCESSION   DASSNH010000036 DASSNH010000000
VERSION     DASSNH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381778"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFK00_01295"
     CDS             <1..300
                     /locus_tag="VFK00_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET7591624.1"
                     /translation="TFLFTLNDKYIVDANVGGNSARWINHSCAPNCCAYIQEVDGARK
                     DKVLIETKRAIRAGEELTYDYGIVLDVPHTAKMKRIWQCRCGSKRCTGTILKPKR"
     gene            395..958
                     /locus_tag="VFK00_01300"
     CDS             395..958
                     /locus_tag="VFK00_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7591625.1"
                     /translation="MSKDALNGRKVAILATDGFEQSELLKPKQLLEDAGASTVVIAPD
                     DKQQIKGWNHGEWGETVEVDRPLGDARAADYDALVLPGGVMNPDKLRLHEEAIEFIRE
                     FGRSGRPLAAICHGPWTLIDAGLAKGKHLTSWPSLRVDLENAGARWDDQEVIVDGSLI
                     TSRKPDDIPAFTRTLIHMLEGRQAQAA"
     gene            complement(1240..2745)
                     /locus_tag="VFK00_01305"
     CDS             complement(1240..2745)
                     /locus_tag="VFK00_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7591626.1"
                     /translation="MKPIDMLRELRNYGGKIRTRGLDDATVEKFAAEDADLAGAIGDA
                     HAAFAELKSRRPEVLGLDEDAQLHALQSGFVNFYPDDAVCPFVPLAARGAWIVTLKGA
                     VLYDCGGYGMLGFGHNPPAVMEALAKPQVMANVMTPSLAQQAFTEALRKEIGHRSGKH
                     PFARFMCLNSGSESVTLAARIADVNAKTMTDPGAKYAGRTIKRLVVKGAFHGRTERPA
                     LYSDSSRKAYQANLASFRDEHSVIVVPPYDVTALRAAFDEAEKNGWFIEAMFLEPVMG
                     EGDPGRAVTPEFYAAARELTAKHDSLLLVDSIQAGLRAHGVLSIVDYPGFEDLPAPDM
                     ETYSKALNGGQFPLSVVAVNERAAALYRKGIYGNTMTGNPRALDVACTVLAQVTPELR
                     NNIRERGVELVKKLDALKDELGGLITKVQGTGLLFSCELSAQFKCFGAGSIEEYLRER
                     GVNVIHGGANSLRFTPHFAINEAEVDLLVANVRKALKEGPRQQAEASRAAA"
     gene            2934..3887
                     /locus_tag="VFK00_01310"
     CDS             2934..3887
                     /locus_tag="VFK00_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7591627.1"
                     /translation="MHAITVAVPVTSPSAIPGEASACTATLSQAAKRYGAATALDGVD
                     LDLHPGELLALLGPNGAGKTTAVSLLLGLQRPDAGEARLFGLDPQRLEARRRIGVMLQ
                     EAALQSTLQVGELIRLTASYYPRPRAFHDIARLAGVEDLLRRRYGKLSGGQKRRVQFA
                     LAICGNPEILFLDEPTVGLDLDARTVMWKTLRELVAGGCSIVLTTHYLEEAEALADRV
                     MVLAHGRIVAAGSVDDIRAHVAQRRIRCMCNLPPATLRAWPEVDLVAHQGEHLEIATT
                     QAEAVLRRLLAADPGLRELEVQRAGLAEAFVEITRDGKGAA"
     gene            3887..>4318
                     /locus_tag="VFK00_01315"
     CDS             3887..>4318
                     /locus_tag="VFK00_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7591628.1"
                     /translation="MQTAIAMPRSRAFRAYLLESRYEFLRLLRTPSFALPTILFPSMF
                     YLLFGVVLAGSERGFDAAKYMLATYSVFGVMAPGLFGFGVVVAMEREQGLLTLKRALP
                     MPPGAYLVAKMVMAMLFAALITLLLMALGFILGHVRMPPGNW"
BASE COUNT          720 a         1412 c         1448 g          738 t
ORIGIN      
        1 actttcctgt tcaccctgaa cgacaagtac atcgtcgatg ccaatgtcgg cggcaacagc
       61 gcgcgctgga tcaaccattc ctgtgccccg aattgctgcg cgtacatcca ggaggtcgat
      121 ggcgcgcgca aggacaaggt gctgatcgag accaagcgcg cgatccgcgc cggggaagaa
      181 ctcacctacg actacggtat cgtgctggac gtgccccaca ccgcgaagat gaagcgcatc
      241 tggcaatgcc gttgcggttc gaagcgttgc accgggacga tcctgaagcc caagcgttga
      301 cgcagtggtc ggtaacgcgt cccgaacgag gccgacacgc gccggtaacg ccggcgctgg
      361 caccgtgatc gtttgccgca caaggagcaa caccatgagc aaggacgcac tgaacggccg
      421 caaggttgca atcctggcca ccgacggctt cgagcaatcc gaattgctga aaccgaagca
      481 actgctggaa gatgccggtg ccagcacggt ggtcatcgcc cccgatgaca agcagcagat
      541 caagggctgg aaccacggcg agtggggcga gacggtggaa gtggaccggc cgttgggcga
      601 tgcacgcgcc gccgattacg acgcgctggt gcttcccggc ggcgttatga acccggacaa
      661 gttgcgcctg catgaagagg cgatcgaatt catccgcgag ttcggccgca gcgggcgtcc
      721 gctcgccgcc atctgccacg gaccctggac gctgatcgat gcggggctcg ccaagggcaa
      781 gcacctgacg tcatggccgt cgttgcgcgt agatctcgag aacgccggcg cgcgctggga
      841 cgaccaggag gtgatcgtgg acggcagcct gatcaccagc cgcaagccgg acgacatccc
      901 ggccttcacc cgcacgctga tccacatgct cgaagggcgc caggcgcaag cggcgtaatg
      961 ggcatccagc ggttcggcgg gtggtgcccg ccctacgcag gcgcaaccga aattcgcatt
     1021 tgtaggttgg cgctgagcgc agcgaagccc aacggttttt aggagtggct ttcgatgtcg
     1081 ttgggcttct ctcggaccaa gtccgggatc aacccaacct acgaagccgc gtaatggcgg
     1141 cactgtccgc cgattgacgc tgcgtcgggc cgcttgcgtg tcctacgcaa aaaaacgccc
     1201 ggcgatgccg ggcgttttgt gcagggtacg tcggcggcgt caggcggccg cgcgcgatgc
     1261 ctcggcctgc tggcgcggcc cttccttgag ggccttgcgg acattcgcga ccagcaggtc
     1321 cacctcggcc tcgttgatcg cgaagtgcgg cgtgaatcgc agcgaattgg cgccgccatg
     1381 gatcacgttg acgccgcgct cgcgcaggta ctcctcgatc gaaccggcgc cgaagcactt
     1441 gaactgggcg ctgagctcgc aggagaacaa caggccggtg ccctgcacct tggtgatcag
     1501 cccgccgagc tcgtccttca gcgcgtcgag cttcttcacc agctcgacac cgcgctcgcg
     1561 aatgttatta cgcagctccg gggtcacctg cgccagcacc gtgcaggcga cgtccagcgc
     1621 gcgcggattg ccggtcatgg tgttgccgta gatgcccttc cggtagagcg cggcggcgcg
     1681 ttcgttcacg gccacgacgg aaagcgggaa ctgtccgccg ttgagcgcct tggagtaggt
     1741 ctccatgtcc ggtgccggca ggtcctcgaa gccggggtag tcgacgatcg acagcacgcc
     1801 atgcgcgcgc aggccggcct ggatcgaatc gaccagcagc agcgagtcgt gcttcgcggt
     1861 gagttcgcgg gcggcggcgt agaactccgg ggtcacggca cggcccgggt cgccttcgcc
     1921 catcacgggt tccaggaaca tcgcctcgat gaaccagccg ttcttttcgg cttcgtcgaa
     1981 cgcggcgcgc aaggcggtca cgtcgtaggg cggcaccacg atcacggaat gttcatcgcg
     2041 gaagctcgcg agattcgcct ggtaggcctt gcgcgatgaa tccgaataca gcgccgggcg
     2101 ctcagtgcgg ccgtggaacg cgcccttgac caccagccgc ttgatggtgc ggccggcata
     2161 cttggcgccc ggatcggtca tggtcttggc gttgacgtcg gcgatccgcg cggcgagggt
     2221 caccgattcg gaacccgaat tgaggcacat gaacctggcg aacggatgct tgccgctgcg
     2281 gtggccgatt tccttgcgca gcgcctcggt gaaggcctgt tgcgccaggc tcggcgtcat
     2341 cacgttggcc atcacctgcg gcttggcgag cgcctccatc accgcgggcg gattgtggcc
     2401 gaagcccagc atgccgtagc ccccgcagtc gtagagcacc gcccccttca gggtcacgat
     2461 ccatgcgcca cgcgccgcca gcgggacgaa cgggcagacg gcgtcgtccg ggtagaaatt
     2521 cacgaagccc gattgcagcg cgtgcaattg cgcgtcctcg tcgagaccga gtacttccgg
     2581 gcggcgcgac ttcaattccg cgaacgccgc gtgggcgtcg ccgatggcac cggccaggtc
     2641 ggcatcttcc gccgcgaatt tctcgacggt ggcgtcgtcc aggccgcggg tgcggatctt
     2701 gccgccgtag ttgcgcagct cgcgcagcat gtcgatcggt ttcatgggac ctcctgccac
     2761 ggcgcccctg cggcgccgta agtggttcaa cgaaaaagaa aatgacctgc aattctagca
     2821 tcgcgggcgt gtttccgtaa cccctcatga cggctgtcag aagcgaaggc tgacgggcgc
     2881 gactgccatg tgctgcgcgc ggccggcaag ctggatgccc gaggaggagc ccgatgcacg
     2941 ccatcactgt tgcagtccct gttaccagcc cgtccgcgat cccgggcgag gcgtccgctt
     3001 gcacggccac gctctcgcaa gccgcgaagc gctatggcgc ggcgacggcc ctggacggtg
     3061 tcgacctcga tctgcatccg ggcgaattgc tggcgttgct cggcccgaac ggcgccggca
     3121 agaccacggc ggtatcgctg ctgctcggtt tgcagcgacc cgatgccggc gaggcgcgcc
     3181 tgttcggcct cgacccgcag cgcctggagg cgcgccgccg gatcggggtg atgctgcagg
     3241 aagcggcatt gcaatccacg ttgcaagtcg gcgagttgat ccgcctgacg gcgagctatt
     3301 acccgcggcc gcgtgccttc cacgacatcg cacggcttgc gggggtggag gatttgttgc
     3361 gacgccgcta cggcaagctg tcgggtggcc agaagcgccg cgtgcagttc gccttggcga
     3421 tctgcggcaa cccggaaatc ctgttcctcg acgagccgac cgtcggcctc gacctcgacg
     3481 cacgcaccgt gatgtggaag acgttgcgcg aactggtcgc cggcggctgt tcgatcgtgc
     3541 tgaccaccca ttacctcgaa gaagccgagg cgctggccga ccgcgtgatg gtgctggcgc
     3601 acggcaggat cgtcgctgcc ggcagcgtgg atgacatccg cgcgcacgtg gcgcagcggc
     3661 ggattcgctg catgtgcaac ctgccgccgg caaccctgcg cgcgtggccg gaggtcgacc
     3721 ttgtggcgca ccagggcgag cacctcgaaa tcgccaccac ccaggccgaa gccgtactcc
     3781 ggcgcctgct cgcggccgat cccggcttgc gcgaactcga ggtgcagcgc gccggcctcg
     3841 ccgaagcctt tgtcgaaatc acccgcgacg gaaagggagc cgcatgatgc aaacagccat
     3901 cgccatgccc cgctcgcgcg cattccgcgc gtatctcctg gagtcgcgct acgaattcct
     3961 gcgcctgctg cgcacgcctt cgttcgcgct gccgacgatc ctgttcccct cgatgttcta
     4021 cctgttgttc ggagtcgtgc ttgcgggctc cgagcgcggc ttcgatgcgg cgaagtacat
     4081 gctcgccacc tacagcgtgt tcggggtgat ggcgccgggg ctgttcggtt tcggcgtggt
     4141 cgtcgcgatg gaacgcgagc aggggctgct gaccctgaag cgcgcgctgc cgatgccgcc
     4201 cggtgcgtac ctcgtcgcca agatggtgat ggcgatgctg ttcgcggcgc tgatcacgct
     4261 gctgctgatg gccctgggat tcatcctggg ccatgtgcgg atgccgcctg ggaactgg
//
LOCUS       DASSNH010000037         9516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_221867, whole genome shotgun sequence.
ACCESSION   DASSNH010000037 DASSNH010000000
VERSION     DASSNH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221867"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFK00_01320"
     CDS             <1..235
                     /locus_tag="VFK00_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591629.1"
                     /translation="YNFACSLSAYLKDREAALDMLKPLFETMTGPLLRYAKADPDLEC
                     LHDDPRYQTMLAAAEARLAATKDPDAPAVEVPA"
     gene            complement(385..2085)
                     /locus_tag="VFK00_01325"
     CDS             complement(385..2085)
                     /locus_tag="VFK00_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591630.1"
                     /translation="MGIVAPLVIGVTSHRNLANREIEGLRSGVRAFFLQLQQDFPGLP
                     LVVLSPLAEGGDQLVAQEALVAGARLVAPFPMPCDEYVRDFSDPSARAQFESLRSKAQ
                     VIELPLLPGNTRADIAVRGAARNRQYAQAGVFTASHCHILLAIWDGRPSDALGGTAQI
                     VRFHMDGIPPGLVERRHSRFVTLDTGDESLLYHIACSRTDGQGGIQGPIPPLQPLQSR
                     WVTDQGVSASDAGIPREFRRMFMNMQQFNADAERYADEFGASSAESPRIAATETLFAV
                     ADRLAIHFQKRVLSAMRGLYALAVLMGFAFVCYSDLPSGVPYMDDAIYVFVVLFAAGV
                     WLAWLAQRREWHRKYIDYRALAEGLRVQGYWQRAGIGADESGAFAHDNFMQKQDVELG
                     WIRNVMRAAAVRASITPPRRDVAAVVAEWIGEEEGEGQLAYYARKVTQRTRAHRDTQL
                     LAQTLLGIVIAASVFLAAFHPWLEPDITSAVVALMGVLAIVAAARESYAYRKADKELI
                     KQYQYMCGIFASARRQLDAAQDPDVQRDILHALGDAALAEHAEWALMHRERPPEHGKL
                     "
     gene            complement(2296..2808)
                     /locus_tag="VFK00_01330"
     CDS             complement(2296..2808)
                     /locus_tag="VFK00_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591631.1"
                     /translation="MLTDNSGCPLVPTAKITVGNLQQNGDGICNNPDFSLANSAECEP
                     KVCMKGNGLDIEITVTGEVDLQFEVAPTERKDKKRRPVPISISFEEEPASNANQQEKN
                     CDEEAELLDPLGLKAFPDRHFVSVNGTTVLVVRDKCAVEGCFKFTLAIQKPGVKKSII
                     IDPKIRNVPM"
     gene            complement(2798..4969)
                     /locus_tag="VFK00_01335"
     CDS             complement(2798..4969)
                     /locus_tag="VFK00_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET7591632.1"
                     /translation="MSESSFDAGRVYRAFISYSHVDELWADWLHKELESWRVPSRLVG
                     TQAAYGVIPRRLAPIFRDRDELASATDLNRTVNAALAKSETLIVICSPDAARSRWVNE
                     EVLAFKRMDREERILCLIVGGEPFASEKPGREAEECFCPALRFMLDASGKPTGERTEP
                     IAADARPGVDSKATAKLRLIAGMLGVGFDTLVQREAHRRHRRMLAITLASILGMAIAA
                     VLSITAYVARNEARQRQAQTEQALNFMLGDLYDKLEGTGRLDLMASVTDKAMALFAAS
                     KPGSLSDLELTQQSRALVQIGQIRIGEGRYGAAMEAFEQAERRSSELTARHADNGRFL
                     YDRAQAEFGIGSLYWQQRKLEAANTWLTRYRDSTLALLKLDPRNDRWRLETTYGEHNL
                     AALALDRGDLVAAQRGFGAELATQRALMREQPSNPQLVADVADTISWLGKLAGLRGDL
                     TDAQRLFSEQARQLADLHSRHPEDFRWLYEWAQAQEVLAASLSAIGQQEESSRILAKA
                     IDANRTLTTHDPKNIPWQISLASAQIRNASYVYAAGHTEQAARLLSEPMGRLQNLGPS
                     TPAHNRQAVHVLSSGWLLSARLAWRGGNLQAASDAATRALMESQNETANDTVEADALA
                     DQADALVLLGMLQQAQSPGTRPPAWAQAHELLAARAATSHNWRVLDPWLRLCLLTGDG
                     AHARIAIDRLNASGYVPLEPWPATAGQPASAATEGDHDHVD"
     gene            complement(5106..5597)
                     /locus_tag="VFK00_01340"
     CDS             complement(5106..5597)
                     /locus_tag="VFK00_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7591633.1"
                     /translation="MSAIRKPARDIRPATSEDKDFILSLVPRFVTFDLPRGRRKRDVM
                     AALRADLEHALREKPAKDHFFIGVGADGDSVGFLHLQVQRDFFSSERTCHVSDIAVAA
                     GHEGRGIGRTLLEHSQRWAKQHRCKLLTLSVFPGNARARALYESAGFAPDLLRMIKRV
                     REP"
     gene            complement(5607..7409)
                     /locus_tag="VFK00_01345"
     CDS             complement(5607..7409)
                     /locus_tag="VFK00_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET7591634.1"
                     /translation="MTTNYTAPLADIHFDLFDLLEVEKLFARLPGCGELTRETVDAVL
                     DEAAKFSEAVLAPLNEVGDRQGCSYDRATGEVTTPDGFKRAYEQYVEGGWAGLTAPEA
                     AGGQGIPEAVGAPVKEMIDSANLAWGTYPLLSHGATEALRVHGEDWQKQAFLTRIASG
                     EWTGTMCLTEAHCGTDLGLLKTRAEPNDDGSHAITGTKIFITAGEHDFTDNIVHLVLA
                     RLPDAPKGVKGISLFIVPKMKVGKDGAMGERNAVRCGGIEHKMGLKGSATCVINFEGA
                     QGWLIGEANRGLNAMFTMMNTARLAVGLQGLAVAERAYQNALSYARERLQMRALSGAK
                     FPDKPADPLIVHGDIRRMLLTQKSLIEGGRALGLYAATLVDIQHRSQDESEARRAEAL
                     VSFLTPIVKALLTENGNECANLALQIYGGHGYIADNGMEQYARDARITTIYEGTTQIQ
                     ALDLLGRKIVQLRGEGLRLFLEQVGAFCAQHADAAVLKEFVSKLSALAREWADVTQRL
                     GQGAASNPEEVGAAAVDYLMYSGYAALAYAWARSVAAARASNQSDDFKQAKLLTARFY
                     FARILPRTRAHFAAMRAGADTLFAMPDALFGSAS"
     gene            complement(7544..8041)
                     /locus_tag="VFK00_01350"
     CDS             complement(7544..8041)
                     /locus_tag="VFK00_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET7591635.1"
                     /translation="MIQSLSRLSARRFAAPAVMAAALALSGCSGLGTKLQPPTAGIQQ
                     LTVQSDGTWVAVVRFQNYSYDTGMHVYAIDATLELDGKPAGHIGFSPGLDIPAMDADT
                     ATATFRPDADGAAALTAGKSSAVAYQLKGTLSAGKSPRGSPQSFKLDGKGFMSPVPGV
                     SNIWR"
     gene            complement(8203..8790)
                     /locus_tag="VFK00_01355"
                     /pseudo
     CDS             complement(8203..8790)
                     /locus_tag="VFK00_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8787..9482)
                     /gene="modB"
                     /locus_tag="VFK00_01360"
     CDS             complement(8787..9482)
                     /gene="modB"
                     /locus_tag="VFK00_01360"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET7591636.1"
                     /translation="MTLLSHAETVALALSFKVSLVGVVCSLPVAIGCAWLLSRKRFFG
                     KPLFEGLLLLPLILPPVVTGYVLLIVFGHHGAIGRLLDAAGLSIAFRWTGAALAAAVM
                     AFPLLVVALRNAFESVDPKLEHAAATLGAGSWRIFFGITLPLSLRGLVAGLALAFARA
                     FGEFGATITFVSSIPGQTRTLPIALYELVSTPGGERGALRLTLVAVVIALLAGLIAAL
                     MLLRGSRNGGAEA"
BASE COUNT         1598 a         3070 c         3160 g         1688 t
ORIGIN      
        1 ctacaacttc gcatgttcgc tgagcgccta tctcaaggat agggaagcgg cgctcgatat
       61 gctcaagcct ttgtttgaaa cgatgaccgg accgctgctg cgatatgcga aggctgaccc
      121 cgacctggag tgcctgcacg acgatccgcg ttaccagacg atgctcgccg ccgccgaagc
      181 acgcctcgcg gcaaccaagg atcccgacgc gccggctgtc gaagtgcccg cgtagccgcg
      241 agcggtcggc ccgcagccgt gacgattcga ccgcggctgt attgtaggag cggcccatgg
      301 ccgcgaccag cttcgcaaga tcactgtagc ccgcattgaa cgccgaacgg gcacccgctc
      361 cgcgcccccg ttcctgcaac aaccttacag cttcccgtgt tccggcggcc gttcgcggtg
      421 catcagcgcc cattccgcgt gctcggcaag cgccgcatcg cccagcgcgt gcaagatgtc
      481 gcgctggacg tccggatcct gcgcggcgtc cagttggcgg cgcgcgctgg cgaagatgcc
      541 gcacatgtac tggtactgct tgatcaattc cttgtcggcc ttgcggtacg cgtaggattc
      601 gcgcgccgcg gcaacgatcg cgagcacgcc catcaaggcg acgacggcgc tcgtgatgtc
      661 cggttctagc cacggatgga acgccgccag gaacacgctc gcggcgatca cgatgccaag
      721 cagggtctgg gcgagaagct gggtatcgcg gtgggcgcgg gtgcgctgcg tgaccttgcg
      781 tgcgtagtag gcaagctgtc cttcgccctc ctcttcgccg atccattcgg cgaccaccgc
      841 cgctacatcg cgccgcggcg gcgtgatgct cgcgcgcacc gcggcggcgc gcatcacgtt
      901 gcggatccac cccaattcga catcctgctt ctgcatgaag ttgtcatgtg cgaaggcccc
      961 ggattcgtcc gcgccgatcc cggcacgttg ccaatagcct tgcacgcgca gcccctccgc
     1021 cagcgcgcgg tagtcgatgt acttgcgatg ccattcgcgc ctttgcgcca gccacgccag
     1081 ccacacgccg gcggcgaaca gcaccacgaa cacgtagatc gcatcgtcca tgtagggcac
     1141 gccactcggc aggtcggagt agcacacgaa ggcgaagccc atcagcacgg ctagcgcgta
     1201 caagccgcgc attgccgata aaacccgttt ctggaaatgg attgccagcc ggtcggcgac
     1261 ggcgaacaag gtttcggtgg ccgcgatgcg cgggctttct gcgctcgagg cgccgaattc
     1321 gtcggcgtac cgctcggcgt cggcattgaa ctgctgcatg ttcatgaaca tccgccggaa
     1381 ctcgcgcggt atgcccgcat cactcgcgct tacgccctgg tcggtgaccc agcgcgactg
     1441 caacggttgc aatggtggga tgggcccctg aatcccgcct tggccgtccg tgcgcgagca
     1501 ggcgatgtga tacaggaggc tttcgtcgcc ggtatcgagc gtgacgaacc tggaatgacg
     1561 gcgttcgacc aggcctggcg ggatgccgtc catgtggaat cgaacgatct gcgcggtgcc
     1621 gccaagcgca tccgaaggac ggccgtccca gatcgccagg aggatgtggc aatggctggc
     1681 ggtgaacacg cccgcctgcg catactggcg gttgcgcgcg gcgccgcgca cggcgatgtc
     1741 cgcgcgggtg tttcccggca gcagcggcag ttcgatcacc tgtgccttcg aacgaagcga
     1801 ttcgaattgc gcccgcgccg aaggatcgct gaaatcgcgc acgtattcgt cgcacggcat
     1861 cgggaatggc gcgaccaggc gcgcgccggc gaccaaggct tcctgcgcga ccagttggtc
     1921 gccgccttcg gcgagcggcg acaatacgac cagcggtaac ccggggaaat cctgttgaag
     1981 ttgcaaaaag aaggcacgca cgccgctgcg caggccttcg atttcccgat tcgcgagatt
     2041 gcggtgactg gtgacaccga tgaccagcgg cgcaacgatc cccacgcttg cctcccctga
     2101 ttggcctatc ggcaccaagc ttcgcccgct cgattatcgc accaactgga gactggcagc
     2161 ccttgcgaga tacgcagggg caggctgtcc gcgagcggca gcagcggttg cctgacctgc
     2221 ctttccaagc cgcggcgggc gcattcctgt ggtggccaag gcaatccacc aaggatgagg
     2281 ccaaaagcgg cgccactaca tcgggacgtt gcggatcttg gggtcgatga tgatcgattt
     2341 ttttacaccg ggtttctgga tggccaaggt gaatttgaaa cagccttcca cggcgcactt
     2401 gtccctcacg accagtactg tggtgccgtt caccgagacg aaatggcgat ccgggaatgc
     2461 ctttagaccg agcgggtcta gcagttcggc ctcctcgtcg cagttttttt cctgttggtt
     2521 ggcgttactt gccggttctt cctcgaagct gatgctgatg ggaacggggc ggcgtttctt
     2581 atccttgcgt tcggtggggg cgacttcgaa ttgaaggtcc acctctcctg tgacagtgat
     2641 ctcgatgtca agcccgtttc ctttcatgca gactttgggc tcgcactccg cactattggc
     2701 taagctgaag tcggggttgt tacaaatacc gtcgccattt tgttgcaaat tgcctacggt
     2761 tatttttgcg gtgggaacga gcgggcatcc tgaattgtca gtcaacatgg tcgtgatctc
     2821 cttctgtggc tgcggaagca ggctgaccgg cggttgcggg ccagggttcc agcggcacat
     2881 acccgctggc attcaggcga tcgattgcaa ttcgtgcgtg ggcgccgtcg ccggtcagca
     2941 ggcacaggcg cagccatgga tccagcacgc gccagttgtg cgaggtggcc gcacgcgcgg
     3001 cgagtagctc atgcgcctgc gcccaggcag gcggacgggt accgggcgac tgcgcttgct
     3061 gcagcatgcc caggagtact agcgcatcgg cttggtcagc caaggcatcc gcttccaccg
     3121 tgtcgtttgc cgtttcgttc tgggattcca tgagcgcccg ggtggctgcg tcgctggcag
     3181 cttggagatt gccgccgcgc catgctagtc gggcgctcag caaccagccg gagctaagca
     3241 catgcactgc ctgcctgttg tgcgccggcg tcgacggtcc gaggttctgc aggcgcccca
     3301 tcggttcgga caacagtctt gcagcctgtt cggtatggcc cgcagcgtag acgtaagagg
     3361 cgtttcgaat ctgggcgctg gccaaggaga tctgccacgg aatattcttt gggtcatgtg
     3421 ttgtcaacgt ccggttggca tcgattgctt tggcgaggat gcgcgagctt tcctcttgct
     3481 ggccaatggc gctgagtgat gcagcaagga cctcctgcgc ctgggcccat tcgtacagcc
     3541 agcgaaaatc ctccgggtgg cggctgtgca ggtccgcaag ttgacgcgcc tgttcgctga
     3601 acaggcgctg ggcgtcggtg agatcaccac gcagtccggc caattttccg agccacgaaa
     3661 tggtgtcggc gacgtctgca accagctgcg gattgctggg ctgttcgcgc atcaaggcgc
     3721 gttgagtcgc cagctcggcg ccgaaacccc gttgtgcggc gaccaggtcg ccgcgatcca
     3781 acgccaaggc ggccaggttg tgttcgccgt aggtcgtttc gagtcgccaa cggtcgttgc
     3841 ggggatcgag cttgagcaat gccaaggtgg aatcgcggta ccgggtcaac catgtgttgg
     3901 ccgcctcgag cttgcgttgc tgccaataga gcgaaccgat tccgaattcg gcttgggcgc
     3961 ggtcgtacag gaaacggccg ttgtcggcgt ggcgcgcggt caattcggaa ctgcgacgtt
     4021 cggcttgttc gaaggcttcc attgccgccc cgtagcgtcc ttcgccgatg cgaatttgcc
     4081 cgatctgtac cagtgcccgc gattgttggg tgagttcgag gtcgctcaac gagcctggtt
     4141 tgctggcggc gaacagtgcc atggccttgt cggtcaccga agccatcaga tcaagccggc
     4201 ccgtgccttc gagcttgtcg tacaggtcgc cgagcatgaa attcagcgct tgctcggtct
     4261 gtgcctggcg ttgccgggct tcgttgcggg caacgtaagc cgttatcgag aggacggcgg
     4321 cgatcgccat cccgaggatc gacgcgagcg tgatggccag catccggcgg tggcggcgat
     4381 gcgcttcgcg ttggaccagc gtatcgaagc cgacccccag catccccgct atcagccgca
     4441 atttcgccgt tgccttgctg tccacaccgg ggcgtgcatc tgcggcgatg ggttcggtgc
     4501 gctcgccggt tggcttgccg ctggcatcga gcatgaagcg cagtgccgga cagaagcact
     4561 cttccgcctc gcggcccggc ttttcgctgg cgaacggctc gccgccgacg atcaggcaca
     4621 agatccgctc ttcgcggtcc atccgcttga atgccagcac ttcctcgttg acccagcggg
     4681 atcgggccgc gtccggtgag cagataacga tcagggtctc ggacttggcc aatgccgcgt
     4741 tgaccgtccg attgaggtcg gtggcgctgg cgagctcatc gcggtcgcgg aagatcggcg
     4801 cgaggcggcg tggaatcacg ccgtaggcgg cttgcgtgcc gaccaggcgg gacggtacgc
     4861 gccaggattc gagttccttg tgcaaccagt ccgcccacag ctcatcgaca tggctgtagc
     4921 tgatgaaggc gcgatagacg cgaccggcat cgaacgacga ctcggacatg gagcgccccg
     4981 ccgaacatgc aaccggaaac cttcacccgg tccgggcttg ccatgatcgg cggctcagcc
     5041 ggggctggag gctggagcct agcacaaatt cagagggccg ctgcgcccgc gccgggcgcc
     5101 ggcggctaag gttccctcac ccgcttgatc atgcgcagca ggtccggcgc aaagccggcg
     5161 ctttcataaa gcgcgcgtgc gcgcgcattg ccggggaaga cggagagcgt caggagtttg
     5221 cagcggtgtt gctttgccca tcgctgcgaa tgctcgagca gcgtgcgacc aatgccgcgg
     5281 ccctcatgtc cggccgccac cgctatatcc gaaacgtggc aggtgcgttc gctggaaaag
     5341 aaatcgcgct ggacctgcag gtgcaggaag ccgaccgaat cgccgtccgc gccgacgccg
     5401 atgaagaagt ggtccttggc cggcttctcg cgcagcgcgt gttcgaggtc ggcgcgaagc
     5461 gcggccatca cgtcgcgctt gcgccggccc cggggcaggt cgaaggtgac gaaacgcggg
     5521 accagcgaca ggatgaagtc cttgtcttct gacgtggcgg ggcggatatc ccgtgcgggt
     5581 tttcggattg ccgacacggc gcgtggctag gaagccgacc cgaacaacgc atccggcatc
     5641 gcgaacaagg tgtccgcgcc ggcccgcatc gcggcgaaat gcgcgcgggt acgcggcagg
     5701 atgcgtgcga agtagaagcg cgcggtcagc agcttggctt gcttgaagtc gtcgctctgg
     5761 ttcgatgcgc gggcggcggc cacgctgcgc gcccaagcat aggcaagcgc ggcgtagccc
     5821 gaatacatga ggtagtcgac cgccgccgca ccgacctcct cggggttcga tgccgcgccc
     5881 tggccgagtc tctgcgtgac gtcggcccat tccctggcca gcgccgacag cttcgaaacg
     5941 aattccttca gcacggcggc gtcggcgtgt tgcgcacaga acgcgccgac ctgttcgagg
     6001 aacaaccgca ggccttcgcc gcgcagttgc acgatcttgc ggcccaggag atccagtgcc
     6061 tggatctggg tggtgccctc gtagatcgtc gtgatgcgcg cgtcgcgcgc gtactgttcc
     6121 atgccgttat cggcgatgta gccgtggccg ccgtagatct gcagcgcgag gttcgcgcat
     6181 tcgttgccgt tttcggtcaa cagcgccttg acgatcggcg tcaggaacga caccagcgcc
     6241 tcggcgcgcc tggcttcgga ttcgtcttgc gagcgatgct ggatgtcgac cagcgtcgcg
     6301 gcatacaggc ccagcgcgcg gccgccttcg atcaatgatt tctgggtcag cagcatccgg
     6361 cggatgtcgc cgtgcacgat caggggatcg gccggcttgt ccgggaactt cgcgccggac
     6421 agcgcgcgca tttgcaggcg ctcgcgcgcg taggaaagcg cattctggta cgcgcgctcg
     6481 gcaaccgcga gcccctgcag tccaacggcg agccgtgcgg tattcatcat ggtgaacatc
     6541 gcattgaggc cacggttggc ttcgccgatc agccagccct gcgcgccctc gaagttgatc
     6601 acgcaggtgg ccgaaccctt gaggcccatc ttgtgttcga tgccgccgca gcgcacggcg
     6661 ttgcgttctc ccatcgcacc gtccttgccg accttcatct tcggcacgat gaacagcgaa
     6721 atacccttga cgcccttggg cgcgtccggc agccgcgcca gcaccaggtg cacgatgttg
     6781 tcggtgaaat cgtgttcgcc ggcggtgatg aagatcttgg tgccggtgat cgcgtggctg
     6841 ccgtcgtcgt ttggctccgc gcgggtcttc aggaggccga gatcggtgcc gcagtgtgcc
     6901 tcggtgaggc acatcgtgcc ggtccactcg cccgacgcga tccgcgtgag gaaggcttgc
     6961 ttctgccagt cctcgccgtg cacccgcaag gcctcggtgg cgccgtgcga gagcagcgga
     7021 taggtgcccc aggccaggtt cgccgaatcg atcatctcct tgaccggtgc gccgacggct
     7081 tcggggatgc cttgcccgcc cgccgcttcg ggcgcggtca atccggccca gccaccctcg
     7141 acatattgct cgtaggcgcg cttgaacccg tcgggcgtcg tgacctcgcc cgtggcgcgg
     7201 tcgtagctgc agccttgccg gtcaccgacc tcgttgagcg gcgccagcac cgcttccgag
     7261 aacttcgcgg cttcgtccag caccgcatcg accgtttcac gggtcaactc gccgcagccc
     7321 ggcagccgcg cgaacagctt ttccacctcc agcaggtcga acaggtcgaa atggatgtcg
     7381 gcgagcggtg cggtgtagtt cgttgtcatt cgtttttccg gcagtggtca atggcgatgg
     7441 tgcacgatag cccatgacac gccgcggcgg gcgatgccca cccgcgagca gtgcggggcg
     7501 gacatcgtcg gcccttgtcg tgtgcacgaa gacaccgggc ccgctaccgc cagatgttcg
     7561 acacccctgg caccggcgac atgaaaccct tgccgtccag cttgaacgac tgcggcgagc
     7621 cgcgcggcga cttgccggcc gaaagcgttc ccttgagctg gtaggcgacg gcgctcgact
     7681 tgcccgccgt gagtgccgcg gcgccgtcag cgtcgggacg gaaggtcgca gtggcggtat
     7741 ccgcgtccat cgcgggaatg tcgagacccg gcgaaaagcc gatgtgcccg gcgggcttgc
     7801 catcgagctc gagggtcgca tcgatcgcgt acacatgcat gccggtgtcg tagctgtagt
     7861 tctggaagcg caccaccgcg acccaggtgc cgtccgattg cacggtcaat tgctggatgc
     7921 ccgcggtcgg cggctgcagt ttcgtcccga gacccgagca gccggacagc gcgagcgcgg
     7981 cggccatcac tgccggcgcg gcgaagcggc gtgcgctgag acgcgaaaga gactggatca
     8041 tcgaccggct cctccagggg cgaggctgcg acggctactt tagcgcgtcg cgcacgggcg
     8101 gcgcacgagt gtggcgcctg ccggcgccgg tgccgacgcc atgaacgcgt cgatgtcctc
     8161 gagcgccgaa agccgcccgt cgccgattcg cgcgacgcca tcggcgagcg ccgcgacttc
     8221 gtcgtactgg tggctcacga ggatcgtggt cagcgcgaat tcctgcttca ggcggcgcag
     8281 gtggtcgagc acttccgcgc gggcctcacg gtgcaggttc gcgagcggtt cgtccagcag
     8341 cagggcttgc ggctggcgca gcagggcgcg cccgatcgct acgcgccggg tctggccgcc
     8401 ggacaactgt ccgggcctgc gtttgagcaa cgtggacagc cccagcaatt gcaccacgtc
     8461 gtcgaatggc ctcgccgtgg tgcttcgcgg catgccgtag cggaggttgt cgcgcacgga
     8521 aagatgcgga aacagccgcg cctcctggaa caccacgccg agcccgcgtt gcgggggcgg
     8581 gaggtccacg ccggcatggc tgtcgaacag cacgtcgcca tccagtgcga tgcggccttg
     8641 ccgcgggcgg agcaggcccg cgatggccag cagcgtcgtg gtcttgccgc agcccgagtg
     8701 cccgaacaat gccagcgcgc gcgcgggcga cgccaacgcg aggtccagaa ggaactcgcc
     8761 gcgctgcagg tggatgtcga ggtcgatcat gcctcggcgc cgccgttgcg cgagccgcgc
     8821 agcagcatca aggcggcgat gagtcccgca agcagcgcga tgaccaccgc gaccagcgtc
     8881 aggcgcagcg cgccgcgctc gccgccgggc gtggaaacca gttcgtacaa cgcgatcgga
     8941 agggtgcgtg tctgacccgg gatcgatgac acgaaggtga tcgtggcgcc gaactcaccg
     9001 aacgcgcggg cgaacgccag cgccagcccg gcgaccagcc cgcgcagcga cagcggcagg
     9061 gtgatgccga agaagatccg ccaggaaccc gcgccgagag tcgccgcggc gtgctcgagc
     9121 ttcgggtcca ccgattcgaa ggcgttgcgc agcgccacca ccagcaacgg gaacgccatc
     9181 accgcagcgg ccagggccgc gccggtccag cggaatgcga tcgacaggcc cgccgcatcg
     9241 agcagccggc cgatcgcacc atggtggccg aacacgatca gcaacacgta gccggtcacc
     9301 accggcggca ggatcaacgg cagcaacagc agcccctcga acagcggttt cccaaagaag
     9361 cgcttgcgtg agagcagcca ggcacacccg atcgcgaccg gcagcgagca tacgacgccc
     9421 accagcgaga ccttgaagct caaggccagg gcgacggttt cggcatggga gaggagggtc
     9481 atctacggct tcgttccagt ccaacaaatt tcactt
//
LOCUS       DASSNH010000038        11146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_595754, whole genome shotgun sequence.
ACCESSION   DASSNH010000038 DASSNH010000000
VERSION     DASSNH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11146
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595754"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /gene="mbhE"
                     /locus_tag="VFK00_01365"
     CDS             <1..2284
                     /gene="mbhE"
                     /locus_tag="VFK00_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HET7591637.1"
                     /translation="LYLPALLLILLGAFTKSAHVPFHFWLPQAMSAPTPVSAYLHSAT
                     MVKAGVFLLLRFWPVLAGTEPWFWIVGGVGLATMLVGAWAATFQHDMKAVLAYSTISH
                     LGLITMLAGLGSTLGVVAAIFHTVNHATFKASLFMAAGAVDHETGTRDLRWLGGLRHV
                     MPITATLAVIAGAAMAGVPLLNGFLSKEMFFAVALGANHGLALDTLTVVVAVAAAALG
                     VTYSLRLVGGAFFGPLKHDLPRKPHEPPLWMRFPIGLLALACLAVGIFPAWAVGPILR
                     AAAASVLGPQLPAYDLAVWHGLTMPMLMSVVAFAAGCALYFLARRRIAARDSTPLIGW
                     IQAMGWFERGLAFLTVTLPALQAKIFPGRKLQVQLLLIVLLAGVLGLLAVQPLPAPVA
                     AHGMHVDPAFALLWLLGVACAIGAATQAKFHRMAALILTAGTGLVVCITFVWLSAPDL
                     AVTQLVVESVTTVLLLLGLRWLPKRIPGLAPLSRIARYRRIRDAVVASGVGLGIAVLA
                     YTVMNHLVADTASRFFLEQALPGGGGRNVVNVILVDFRALDTLGEITVLAIVALAVFA
                     LLWRFRPAVESIGTPRKRDEAPVHDEVQVGRERTALADQLAIPGLLILLVAPLITLFA
                     LHLFLRGHDLPGGGFVAGITVTMALIVLYMAAGARRVEARLKVAPVRWIGAGLLLAIA
                     TGVAAVVLGEPFLTSHHGHWTLPVLGNVPFNSALLFDLGVFAVVSGASALILVVLAHQ
                     SLRRVIHVPDAGRDAGGAGT"
     gene            2284..2625
                     /locus_tag="VFK00_01370"
     CDS             2284..2625
                     /locus_tag="VFK00_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HET7591638.1"
                     /translation="MEFVLAIAIGALAGSGVWLLLRPRTFQVIIGLTLISYAVNLFIF
                     SIGGVRVGADPVLAGGATDLSRYADPLPQALVLTAIVIGFATTALFLVVLLTSRGLAG
                     NDHVDGEDGTP"
     gene            2622..4202
                     /locus_tag="VFK00_01375"
     CDS             2622..4202
                     /locus_tag="VFK00_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HET7591639.1"
                     /translation="MNASLAIAPILIPLAVAAVQILLGTRSRHLRTGLSFLSCSALVA
                     LAAALMVSASAPDSEGTTLVYNLGSWPATLAIVLVVDRLSALMLLLTAVLALAVLPYA
                     LGRWQRMGPHFQPLVQLLLVGYNGAFLTGDLFNLFVFFEVLLAASYGLALHGSGARRV
                     GAGLHYIAINLTASMLFLVGVGLIFGVTGTLNMAVLAHLVPGLTGTPLALMHAGAALL
                     GLAFLIKAAVWPVGFWLPRTYSAAIPPVAALFAAMTKLGIYVILRLSLLLFGADAGAS
                     SAHFGSEWLLALGITTLVVGSIGMLGAREVGKLAAYCVLVSSGTLLGAIALHSPAVTA
                     GALAYLVISVLGIAALFLLAGLVAPREVAEGAAQLESYDPAVDALFAAEDERRVTSPA
                     PVVILAACFLACGLLLAGLPPLSGFIAKFAMLAPMLGGGPGATLLFALAIAAGLCTVI
                     AMARAGIQVFWADADWEFPRVEAGETISVVGLLGICLVLTFVVAAPWGYLAATARQIH
                     APERYIHAVMGQAAEGQP"
     gene            4199..4684
                     /locus_tag="VFK00_01380"
     CDS             4199..4684
                     /locus_tag="VFK00_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HET7591640.1"
                     /translation="MTRWLPHPWVALWLLVAWLLLQQSLAASTIVPGIVLALVLSWAL
                     GKLDLPRVRVRSLGILLLLLAQVFGDIIRSNIAVAKALVSPELRVTSGFVSIPLEITS
                     PHALALLACIITATPGTVWVSHDSGRRVLVIHVFDLVDESIWIANIKRRYEQPLLRIF
                     Q"
     gene            4681..4962
                     /locus_tag="VFK00_01385"
     CDS             4681..4962
                     /locus_tag="VFK00_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HET7591641.1"
                     /translation="MIPSLATTVLVVSQFLLLLGMGMAIIRMVRGPRAQDRVLALDAL
                     YIDGMLLLLTIGIRTGSILYVEAALIIALLGFAATAAFAKFLLRGEVIE"
     gene            4959..5342
                     /gene="mnhG"
                     /locus_tag="VFK00_01390"
     CDS             4959..5342
                     /gene="mnhG"
                     /locus_tag="VFK00_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HET7591642.1"
                     /translation="MNALAALPPWIALATAILVFAGALLACIGSAGLLRLDTFYRRVH
                     APTLGTTLGMYLVLAGTIVFFWAGKGQPALHVVLVGVCVSVTTPISLMLLARAALERD
                     RRAGMAGVPAAASSESGHENHSSGR"
     gene            complement(5369..6823)
                     /locus_tag="VFK00_01395"
     CDS             complement(5369..6823)
                     /locus_tag="VFK00_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET7591643.1"
                     /translation="MFSLALPALGRASGIPPVDPGNSDLGAVIELVTAGQFGAADARI
                     AAALKQADLSPGARRAFEFQRERMQRMRKDFSLTADQVKAKVRKQIPGLTDAEFEDWD
                     AHHLFEHMAIDGQRLYFNRSPSNLFHLSAAAVARRTAQPGFVGSGLPKEVNDNYIDYD
                     RKVIEQAKVTGQTSVLPQRVRVTQTLTVDTDAVPAGKTVRAWIPYPRADMGQQEDIVF
                     LSSQPTVHQIAPESALQRTVYLEQTAQAGKPTVFKITYEVTLYAQYHAIDPSKVMPEK
                     ITPELAPYVAERAPHVVFTEPLRVFSKQVVGDATDPYEIARRIYAAVDAIPWAGAREY
                     STIPDISAYTLHAGHGDCGEQTLLLVTLMRMNGIPARWQSGWTFTKGDYNNIHDWAEI
                     HLAPYGWVPVDVTYGRLASDDPALEWFYLGGLDNFRIAFNDDFSRDFVPAKQHFRSDT
                     VDSQRGEVEWDGGNLYYDKWDYHFDWKILPAISG"
     gene            complement(7012..8907)
                     /locus_tag="VFK00_01400"
     CDS             complement(7012..8907)
                     /locus_tag="VFK00_01400"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HET7591644.1"
                     /translation="MKRLSLFLCALAFVVAPPAMASEGPGVPGPVALDVSLPDPGQKI
                     LYVHETMPVQPGSLTLYYPKWIPGDHSPDGPINDIMGLEFSANGKRIAWQRDELDRFT
                     FHLTVPQGVHQLDISFQFPSRDRVTDHLIDLTWDHVSLYRAGFPTKDQMFKPSLSVPA
                     DWHYGTALQTDEREGQRIDFKPVPFNTLVDSPVIAGKYFREIDLTPKGSSVHRYLDMV
                     GDSAAAVAISDQQRAGFHHLIEQAQALFQSHHYDSYHLLLTLSDHTAKGGLEHHQSSD
                     DKARGGSRMFADPAHFMLDGSLLPHEYTHSWNGKFMRPVGLWRADFEQPEKPRLLWVY
                     EGLTVYLGDTLAARSGLWSPETWRDALAYRAAVMAHRTGREWRPLVDTTIAVDYGAPE
                     AWASWRRQNDFYREGELLWLAVDMKIRDLSRGTRDIDDFASAFFGIDNGSFVTRTYDF
                     DDVVKALGKIQPYDWATFLHDWVNGTGDQVPLLSGIEASGWELVYTDKPSAYESALEN
                     VGMGELEGHGINAMFSIGMFLSTDGRVEDVLWQGPSFKAGLAPGMKLVSIDGHAWSAQ
                     VLHDAIAAAQKNEQALKIQARSDGAVKTYTVDYHDGLKYPHLVREQGKPDYLKALLAP
                     KPVGGGA"
     gene            complement(8957..10297)
                     /locus_tag="VFK00_01405"
     CDS             complement(8957..10297)
                     /locus_tag="VFK00_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7591645.1"
                     /translation="MTAPSAPPRELPRKLGLVTGMAVVVGVIIGSGIFRVPSGIAADT
                     GNLTGIALVWILGGIVALFGALSIAELAAMYPAAGGPYVYLREAYGRPLAFLFGWMFL
                     LTQPISWAAQALMFSEYLGFFVPIPEFTQHLVAAALIVLVAAANYRSVNLGAAIQNLS
                     TAAKVLAIVGLSATIFFLAPGGEHNPLNAEPMGAAKWSGIGIGLIAALWAYDGWENLT
                     TLSGEVKNPGRNLPIALIGGVILVMVVYLLINVAYLRALPLPELAASKSVATDAASVV
                     LGRIGVSLVGALVMLSVFGTLNGSILSTPRVFFAMAEDGLFFRTVGKIHPKYETPYVA
                     IAFIVVIAVVYVLLRDFMQLAEAYVLGIWPFLALCVIGLFILRSKRPAVPRPYRTLGY
                     PVIPALFVLATFVVVANALYAQFWSTIASILITLVGLPLYYLWMWRQRRHGHAV"
     gene            10945..>11146
                     /locus_tag="VFK00_01410"
     CDS             10945..>11146
                     /locus_tag="VFK00_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591646.1"
                     /translation="MKSFRRISRRNKLCAAVAVCLSLMAAPAVMAQDVTGSVVGRTAS
                     GATVTISNPATGFTRKVTAANDG"
BASE COUNT         1736 a         3632 c         3677 g         2101 t
ORIGIN      
        1 gctgtatctg ccggcgctgc tgttgatcct gctgggcgcg ttcaccaaga gcgcgcacgt
       61 gccgttccat ttctggctgc cgcaggcgat gtcggcgccc acgccggtgt cggcgtattt
      121 gcactctgca acgatggtga aggccggtgt gttcctgctg ctgcggttct ggccggtgct
      181 ggcgggcacg gagccgtggt tctggatcgt gggcggggtc gggctcgcga cgatgctggt
      241 cggcgcctgg gccgctacgt tccagcacga catgaaagcg gtgctggcgt attccacgat
      301 cagccacctc ggtctgatca cgatgctggc cgggctcggc agcaccctgg gcgtggtggc
      361 ggcgatcttc cacaccgtca accatgccac cttcaaggcc tcgttgttca tggccgcggg
      421 tgcggtggac cacgaaaccg gcacccgcga tttgcggtgg ctgggtggcc tgcgccacgt
      481 gatgccgatc accgcgacct tggccgtgat tgccggggcg gcgatggccg gcgttccgtt
      541 gctcaacgga ttcctgtcca aggaaatgtt tttcgcggtg gcactcggtg ccaaccacgg
      601 cctggcgctg gacacgttga cggtggtggt cgcggttgcc gcggctgcgc tcggcgtaac
      661 ctattcgctg cggctggtgg gcggcgcgtt cttcggcccg ctcaagcacg accttccccg
      721 caagccgcat gagccgccgc tctggatgcg ctttccgatc gggttgctgg cgctggcgtg
      781 tcttgcggtc gggattttcc cggcgtgggc ggtcggtccg atcctgcgcg cggccgcggc
      841 ttcggtactg ggtccgcaat tgcccgcgta cgatctcgcg gtctggcatg gcctgacgat
      901 gccaatgctg atgagcgtgg tcgcgttcgc ggcgggatgc gcattgtatt tcctggcgcg
      961 acggcgcatc gccgcgcgcg acagcacgcc gctgatcggc tggatccagg ccatgggctg
     1021 gttcgaacgg gggcttgcgt tcctgacggt caccctgccc gccttgcagg caaagatctt
     1081 tccgggccgc aagctgcagg tgcaattgct gctgatcgtg ctgctggcgg gcgtcctcgg
     1141 cttgctggcg gtgcagccgt tgccggcgcc ggtggcagcg cacggcatgc acgtcgaccc
     1201 ggcgttcgca ttgctgtggc tgctgggcgt ggcgtgcgcg atcggcgcag ccacccaggc
     1261 caagttccat cgcatggcgg ccctgatcct cactgccggg acgggcctcg tggtgtgcat
     1321 caccttcgtg tggctgtcgg cgcccgacct cgcggtaacg caactggtgg tcgaatcggt
     1381 cacgaccgta ttgctgctgc tgggactgcg ctggctgccc aagcgcattc cgggactggc
     1441 gccgctgagc cgcatcgcgc gctaccgtcg catccgcgac gcggtcgttg cgtccggcgt
     1501 cggcctgggc atcgccgtcc tggcgtacac ggtgatgaat cacctggtcg ccgataccgc
     1561 atcgcgcttc tttctcgaac aggccttgcc gggcggcggc ggacgcaacg tggtcaacgt
     1621 catcctggtg gacttccgcg cgctggacac gctgggcgaa atcaccgtgc tggccatcgt
     1681 ggcgctggcg gtgttcgcgc tgctgtggcg gttccggccg gcggtcgaga gcatcggtac
     1741 tccacgcaag cgcgacgaag caccggtgca tgatgaagtc caggtcggac gtgagaggac
     1801 cgcattggcc gatcaactgg ccatccccgg gttgctgatc ctgctggtcg caccgttgat
     1861 cacattgttc gcgctgcact tgttcctgcg cggccacgac ctgccgggcg gcggattcgt
     1921 cgccggcatt accgtgacga tggcactgat cgtgttgtac atggccgccg gtgcgcgccg
     1981 ggtggaagcg cgtctcaagg tcgcgccggt gcggtggatc ggcgcgggat tgctgctggc
     2041 gatcgccacc ggggtggcgg cggtcgtact cggcgaaccg ttcctcacct cgcaccacgg
     2101 gcattggacc ctcccggtgc tcggcaacgt tccgttcaac agcgcgctgc tgttcgacct
     2161 cggcgtcttc gcagtcgtgt ccggcgccag cgcgctgatc ctggtggtgc tggcgcacca
     2221 atcgttgcga cgcgtgatcc atgttcccga cgcgggtcgc gacgccggag gagccgggac
     2281 ctgatggaat tcgtgctggc aatcgctatc ggcgcgctcg cgggttcggg cgtgtggctg
     2341 ctgctgcggc cgcgcacctt ccaggtgatc atcggcctga cgctgatttc ctacgcggtg
     2401 aacctcttca tcttctcgat cggtggcgtg cgggtcggtg cggacccggt gctcgccggc
     2461 ggtgccaccg atctttcccg ctatgccgat ccgctgcccc aggctctggt gctgacggcg
     2521 atcgtgatcg gcttcgcgac cactgcattg ttcctggtgg tgctgctgac gtcgcgcggt
     2581 ctcgccggca acgaccacgt cgatggcgag gacggcacgc catgaatgcc tcgctcgcga
     2641 tcgcaccgat cctgattccg ctcgcggtcg ctgccgtcca gatcctgctc ggcacccgct
     2701 cgcggcacct gcgcaccggt ctgagcttcc tgtcgtgctc cgcgctggtc gccctagccg
     2761 cggcgctgat ggtgtcggca tcggcgccgg atagcgaagg gacgacgctg gtgtacaacc
     2821 tcgggagctg gccggcgacg ctcgcgatcg tgctggtggt cgaccggttg tcggcgctga
     2881 tgctgctgct caccgcggta ctggcgctcg cggtgttgcc gtacgcgctc ggacgctggc
     2941 agcgcatggg tccgcatttc cagccgctcg tgcagttgct gctggtcggc tacaacggcg
     3001 cgttcctgac cggcgacctg ttcaacctgt tcgtgttttt cgaggtgctg ctggccgcgt
     3061 cctacggact ggcgttgcac ggctccggcg cgcgccgggt cggggccggc ctgcactaca
     3121 tcgcgatcaa tttgaccgcc tcgatgctgt tcctggtcgg cgtgggcctg atcttcggcg
     3181 tcaccggcac cttgaacatg gcggtgctgg cgcacctggt tcccggcctc accggcacgc
     3241 cgcttgcgct gatgcacgcg ggcgcggcat tgctcgggct cgccttcctg atcaaggcgg
     3301 cggtgtggcc ggtgggtttc tggctgccgc gcacgtactc ggccgccatc ccgcctgtcg
     3361 ccgcgctgtt cgcggcgatg accaagctcg gcatctacgt gatcctgcgc ctgagcctgc
     3421 tgttgttcgg cgccgatgcc ggcgcgtcgt ccgcgcattt cggcagcgag tggctgcttg
     3481 cgctcgggat caccacgctg gtggtgggca gcatcggcat gctgggcgcg cgcgaagtcg
     3541 gcaagctcgc cgcgtactgc gtgctggtgt cgtcgggcac actgctgggg gcgattgcgc
     3601 tgcacagtcc ggccgtgaca gcgggcgcgc tggcgtacct cgtgatttcg gtgctcggga
     3661 tcgccgcgct cttcctgctg gccggcctgg tggcgccgcg cgaggtagcc gaaggagcag
     3721 cgcaactgga atcctacgat ccggccgtcg atgccttgtt cgccgccgag gacgaacggc
     3781 gggtgacgag tccggcgccc gtcgtgatcc tcgccgcctg cttcctcgcc tgcggattgc
     3841 tgctggcggg actgccgccg ctctccggtt tcatcgcgaa gttcgcgatg ctggcaccga
     3901 tgctgggcgg ggggcccggc gccacgctgc tgttcgcgct ggcgattgcc gcgggcctct
     3961 gcacggtgat cgcgatggcg cgcgccggca tccaggtttt ctgggccgat gccgactggg
     4021 aatttccgcg cgtcgaggcg ggcgagacaa tctcggtggt cgggcttctg ggcatctgcc
     4081 tcgtgctcac cttcgtggtc gccgcaccct ggggttacct tgcggcgacc gcacgccaaa
     4141 tccatgcgcc cgagcgctat atccacgcgg tgatgggcca ggcggcggag ggccagccat
     4201 gacacgctgg ttaccacacc cgtgggtcgc cctgtggctg ctggtggcgt ggctgttgct
     4261 gcagcaaagc ctcgccgcgt cgacgatcgt gcctggcatc gtcctggcgc tcgtgctgtc
     4321 ctgggcgctc ggcaaactgg acttgccgcg ggtgcgcgtg cgcagcctcg gaatcctgct
     4381 cttgctgctc gcgcaggtat tcggcgacat catccgttcc aatatcgcgg tggcgaaggc
     4441 gctcgtctcg cccgagctgc gcgtcacttc ggggttcgtt tcaatcccat tggaaatcac
     4501 cagtccgcat gcactggcgt tgctggcatg catcatcacc gcaaccccgg gcacggtctg
     4561 ggtcagccac gattcggggc gccgcgtgct ggtgatccac gtgttcgacc tcgtcgatga
     4621 atccatctgg atcgccaaca tcaagcgccg ctacgagcag ccgctgctca ggatcttcca
     4681 atgatcccga gcctcgcgac caccgtcctc gtcgtttccc aattcctgct gttgctgggc
     4741 atggggatgg ccatcatccg gatggtgcgc ggcccgcgcg cgcaagaccg ggtgctggca
     4801 ctggatgcgt tgtacatcga cggcatgctg ctgttgctga ccatcggcat ccgcaccggc
     4861 agcatcctgt atgtcgaagc cgcattgatc atcgcactgc tcggtttcgc ggccacggcg
     4921 gcgttcgcga aattcctgct gcggggcgag gtgatcgaat gaatgcgctc gcggctttac
     4981 caccctggat cgcgctggcg acggccatcc tggtgttcgc cggggcgctg ctggcatgca
     5041 tcggttcagc cggcctgctg aggctggaca ccttctaccg gcgcgtgcac gcgccgacgc
     5101 tcggcaccac gctcggcatg tacctggtgc tcgccggaac gatcgtgttc ttctgggctg
     5161 gcaagggcca gccggcgctg cacgtcgtcc tggtcggcgt gtgcgtcagc gtgaccacgc
     5221 cgatctcctt gatgctgctg gcgcgcgctg cactggaacg cgacaggcgg gcaggcatgg
     5281 ccggcgtacc ggccgccgct tcgagcgagt ccgggcacga gaaccactca tcgggtcgtt
     5341 gactcaagcg cggcatccgg cggtgccttc agccgctgat ggctggcagg atcttccagt
     5401 cgaagtggta gtcccacttg tcgtagtaga gattcccgcc gtcccattcg acttcgccgc
     5461 gttgcgagtc gacggtgtcg gagcggaagt gctgcttggc ggggacgaaa tcgcgcgaaa
     5521 aatcgtcgtt gaaggcaatc ctgaaattgt ccagtccgcc gaggtagaac cattccagtg
     5581 ccggatcatc ggaagcgagc cgtccatagg tcacgtccac cggaacccat ccgtacggcg
     5641 cgagatggat ttccgcccag tcatggatgt tgttgtagtc gcccttggtg aaggtccacc
     5701 ccgattgcca acgcgccgga atgccgttca tccgcatcaa cgtgaccaga agcagggtct
     5761 gctcgccaca gtcaccgtgg ccggcgtgca gggtgtaggc gctgatatcc ggaatggtgg
     5821 agtattcgcg cgcgccggcc catggaatcg cgtccacggc ggcgtagatg cgtcgggcga
     5881 tctcataggg atcggtcgcg tcgccgacca cctgcttcga gaacacccgc agcggctcgg
     5941 tgaacaccac gtgcggcgcg cgctcggcca cgtacggtgc aagttccggc gtgatcttct
     6001 ccggcatcac cttggacgga tcgatcgcgt ggtattgcgc gtacagcgtg acctcgtagg
     6061 tgatcttgaa taccgtcggc ttgccggctt gtgcggtctg ctccaggtac acggtgcgtt
     6121 gcagcgccga ctccggcgcg atttgatgta cggtcggctg gctggaaaga aacacgatgt
     6181 cttcctgctg ccccatgtcg gcacgcggat acggaatcca cgcgcgcacg gtcttgccgg
     6241 ccggaacggc gtcggtatcc acggtgaggg tctgcgtcac ccgcacccgc tgcggcagca
     6301 cgctggtttg gccggtgacc ttcgcctgct cgatgacctt gcggtcgtaa tcgatgtagt
     6361 tgtcgttgac ttccttcggt aggccgctgc cgacgaaccc gggttgcgca gtgcgtcgcg
     6421 ctaccgcggc ggcactcaag tggaacaggt tggaaggcga acggttgaag tacagccgct
     6481 ggccgtcgat cgccatgtgt tcgaacaaat ggtgcgcgtc ccaatcctcg aattcggcat
     6541 cggtgagacc aggaatctgc ttgcgcacct tcgccttgac ctgatcggcg gtcaggctga
     6601 aatccttgcg catgcgttgc atgcgctcgc gctggaattc gaacgcgcgt cgcgccccgg
     6661 gcgacagatc cgcctgcttc agcgccgcgg cgatgcgtgc atcggcggcc ccgaattgcc
     6721 cggccgtgac gagttcgatc accgccccca ggtcgctgtt gcccggatcg acgggtggaa
     6781 ttccggacgc ccggccgagc gcgggtagtg cgagcgagaa catcaacgcc agcaacgcgg
     6841 cacatcggaa cgcgggaagg aatccttgat ctcgatcgtc atccgcgcgg acgccgggat
     6901 ccgtgttgct ttgctgtgcc agccccgaga tcggcacgga tcccgtgttc ggcccgcgat
     6961 gaatccgcgc ggagtccccg gaggacggtg aaaaccgata cccggacgtt ttcatgcgcc
     7021 acccccgacc ggtttcggcg ccagcaatgc cttcaggtaa tcgggcttgc cttgctcgcg
     7081 cacgaggtgc gggtacttga gtccgtcgtg gtagtccact gtgtaggtct tgacagcgcc
     7141 atcgctgcgc gcctgtatct tcagcgcctg ttcgttcttc tgcgccgccg cgatcgcatc
     7201 gtgcagcacc tgcgcgctcc aggcatggcc gtcgatggaa acgagcttca tgccgggcgc
     7261 aagccccgcc ttgaacgacg gtccctgcca caacacgtcc tcgacccggc catccgtgga
     7321 aaggaacatg ccgatggaga acatcgcgtt gatgccgtgg ccttccagtt cgcccattcc
     7381 gacgttttcc agcgcgcttt cgtaggctga cggtttgtcg gtgtagacca gttcccagcc
     7441 gctggcctcg atgccggaca gcagcggcac ctggtcgccc gtgccgttta cccagtcatg
     7501 caggaacgtt gcccaatcgt acggctggat tttgccgagc gccttcacga catcgtcgaa
     7561 atcgtaggtg cgggtgacga aactgccgtt atcgatgccg aagaaggcgc ttgcgaaatc
     7621 atcgatgtcg cgcgtgccgc gactcaggtc gcggatcttc atgtccacgg ccagccacaa
     7681 cagctcgccc tcccgataga aatcgttctg gcgacgccaa ctggcccacg cttcgggcgc
     7741 gccgtaatcc accgcgatgg tggtgtcgac gagcggacgc cattcacggc cggtgcggtg
     7801 cgccatcacg gcggcacggt acgccaacgc gtcgcgccag gtttcgggcg accacaggcc
     7861 gctgcgtgcg gccaaggtgt cgccgaggta caccgtcagc ccttcgtaca cccacagcag
     7921 cctgggcttt tcgggctgtt cgaaatcggc ccgccacagc cccaccgggc gcatgaactt
     7981 gccgttccaa gaatgcgtgt attcgtgcgg cagcagggaa ccgtcgagca tgaaatgcgc
     8041 ggggtcggcg aacatcctcg aaccgccgcg cgccttgtcg tcgctcgact gatgatgctc
     8101 gagtccaccc ttggcggtgt ggtcgctcaa cgtcagcaac aggtggtagc tgtcgtaatg
     8161 atgcgactgg aacagcgcct gtgcctgttc gatcaggtga tggaaacccg cgcgttgctg
     8221 atccgagatc gcgaccgctg ctgcgctgtc gcccaccatg tcgaggtagc gatgcaccga
     8281 cgaacccttg ggtgtcaggt cgatttcgcg gaaatacttg cccgcgatca ccggcgaatc
     8341 aaccagcgtg ttgaatggca ccggcttgaa gtcgatgcgc tggccttcgc gctcgtcggt
     8401 ctgcagcgcg gtgccgtagt gccagtcggc ggggaccgac agcgacggtt tgaacatctg
     8461 gtccttggtc ggaaatcctg cgcgatacag cgagacgtgg tcccaggtga ggtcgatcag
     8521 gtggtcggtc acgcgatcgc gcgacggaaa ctggaagctg atgtcgagct ggtgcacgcc
     8581 ttgcggcacc gtcaaatgga acgtgaagcg gtcgagttcg tcgcgttgcc acgcgatgcg
     8641 ctttccgttc gcggaaaact ccaggcccat gatgtcgttg atggggccat ccggcgaatg
     8701 gtcgccgggg atccacttcg ggtagtacag cgtcaacgac ccgggctgga caggcatcgt
     8761 ttcatgtacg tagaggattt tctggccggg atcgggcagg ctcacgtcca gggcgacggg
     8821 accgggcacc cccggacctt ccgaggccat ggcgggtggc gccaccacga acgccaacgc
     8881 gcataggaac aacgacaatc gtttcatgcc tccctccgat gttctgtggt tcgagctgta
     8941 cgccaccgcc ggagcgtcac acagcgtggc catgcctccg ctgccgccac atccacaggt
     9001 aatacagcgg caagccgacc agcgtgatca ggatgctggc gatggtcgac cagaactgcg
     9061 cgtacaacgc gttggcgact accacgaagg tcgccagcac gaacagcgcg ggaatcaccg
     9121 gatagccgag cgtgcgatac ggacgcggaa cggccggccg cttgctgcgc aggatgaaca
     9181 ggccgatcac gcacaacgcc aggaagggcc agatgcccag cacgtaggct tctgcaagct
     9241 gcatgaaatc acgcagcagc acgtacacta ccgcgatcac gacgatgaat gcgatcgcga
     9301 cgtaaggcgt ttcgtatttc ggatggatct tgccgacggt gcggaagaag aggccatcct
     9361 cggccattgc gaaaaacacc cgcggggtcg ataggatgct gccgttgagc gtgccgaaca
     9421 ccgacagcat caccagcgcg ccgaccagcg agaccccgat gcggcccagc accaccgagg
     9481 cggcgtcggt agccacggac ttcgaggccg ccagttcggg caacggcaag gcgcgcaggt
     9541 aggcgacgtt gatcagcagg taaaccacca tcaccaggat gacgccacca atcagcgcaa
     9601 tcggcaaatt gcggcccggg ttcttcacct cgcccgacag cgtggtcagg ttttcccagc
     9661 catcgtaggc ccacaacgcc gcgatcaggc cgatgcctat ccccgaccat ttcgccgcgc
     9721 ccattggctc ggcgttaagc ggattgtgct cgccacccgg cgcgaggaag aagatcgtcg
     9781 ccgacaaccc cacaatcgcc agcaccttgg cagcggtcga caggttctgg atcgcagcgc
     9841 cgaggttcac cgaacggtaa ttggccgcgg cgaccagcac gatcagcgcc gccgccacca
     9901 ggtgctgggt gaactccgga atcggcacga agaatcccag gtactcggaa aacatcaacg
     9961 cctgcgccgc ccacgaaatc ggttgggtca acaggaacat ccagccgaac aggaacgcca
    10021 gcggccgtcc gtaggcttca cgcagataca cataagggcc acccgcagcg ggatacatgg
    10081 ctgccagttc cgcgatcgac aacgcgccga acagtgccac gatgccgccg agaatccaca
    10141 ccagcgcgat gccggtgaga ttgccggtat ccgccgcgat gccggacggc acccggaaga
    10201 ttccgctgcc gatgatcacc cccaccacca cggccatgcc ggtgacgagg ccgagcttgc
    10261 gcggcagttc gcgcggaggc gcagacggtg cggtcatggg cgagcatcgt acatgcaagc
    10321 agcagctgtg tagaggcgaa cctgggcgcg ctcggcgcac gtctgttgct gcacccgcac
    10381 acacgcgatc gcgcggcagc cagtgggctc caggcgaatt ccgcgcgtgg cccgcgtaac
    10441 gaaaccataa cccgcagcta tgccgggtgc ctgtcttttc attggtggtt tgcgctggcc
    10501 ggtgagtatg ctcaaggaca taccgcgatg gcccgaaggc cctgcggtgg cagccgtcac
    10561 gcaagcgcga ccatgcccct gaccgaggcg acattcgcac attccgcgcg acgccaggga
    10621 agcgagacgt gggctgcgca acgtgtcgaa ccgttaccgg ctggggagta cgcgtgatga
    10681 agagcaggtt cctgcatgga tcgcggcgca tgatccgcac ctcacgcccc ccggcgaatg
    10741 aagccgccat cctcccgcgc ggagcgacgc cgaatcgaca tcgctccgac gcgatcccgc
    10801 atgtcaccgc gtgccccagg cgcgcgaggc gcgatcgtcg cactggatcc gtcgcccacc
    10861 actccacgcg gagcattgtg ccgacgcgac gcccttgaat tcctgaccgc cctttccacg
    10921 cagcacgcca gaccggagcg caccatgaaa agctttcgac gcatttcacg ccgcaacaaa
    10981 ctctgtgccg cagtggccgt ttgcctgtcc ctgatggcgg ctccggccgt catggcacag
    11041 gacgtgaccg gttcggtcgt gggtcgcacc gcatccggcg caacagtgac gatcagcaat
    11101 cccgcaaccg gctttacccg caaggtgacg gctgccaatg atggca
//
LOCUS       DASSNH010000039        17368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1472009, whole genome shotgun sequence.
ACCESSION   DASSNH010000039 DASSNH010000000
VERSION     DASSNH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 17368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17368
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472009"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2616
                     /locus_tag="VFK00_01415"
     CDS             <1..2616
                     /locus_tag="VFK00_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7591647.1"
                     /translation="FGGSFWEFGEPDWDRTKYLMLWGVAEDHDSNPIKLGLGKMKARG
                     AKIVAINPVRTGYGSIADEWVPVNPGTDGLLAGALIHELLRNDRVDFEYLVRYTNAHH
                     LVIRDPDAADDGLIARDADGKPLCAARAGTPQPAARPGAAPSGLRATSSGGGAFSLFP
                     PGEGARRADEGATPPYRVVLADAAGILPMVVGEYALHDGRRAVPAFQLLAERFLAQEY
                     APENVSKRCGVPASTIRRLAAELAHVAFDEAIELPIAWTDTHGRRHAAMVGRPVSMHA
                     MRGIAAHANGFHTCRTIHVLQMLLGAIDTPGSFRYQPPYPKPAPPGNKPARARKPDGT
                     LTGNPLGYPQSPDDLLVDAEGKPRRIDHAFSWEYPLAAHGLLQSVIRNAVAGDPAKID
                     TLFLFMANMGWNSAMDTANTRKLLCERGADGEYKIPRIIYADAYDSETVAFADLVLPD
                     TTYLERYDCMSLLDRPISDAESAADAIRRPVVAPDRDVRPFQDVLLELGARLKLPNMA
                     DSDGTSVYQDYADYMVRHERAPGVGMLTGWRGADGMQAGKGAANPDQLQRYIEHGCFF
                     EARVPEAGAYYRMANRDYLAWAQSMGFVASTEPVTMQLYCEVLQRFRLAAQGHGAVQP
                     PERERARVARYFDPVPMWYSSQSDTDSPLPSGEGDKRRVRDADGISRRFERAAKDGRS
                     GAPHHGGLLAEFEASPGASHRRKGGAPLLPEGAGKAEFPLSAISQRPMFMYHAWGSQN
                     RWLRQIAARNVLYVHPDALAAAAIADGDWAWIASPRGRLRVQVRAHAGCAPGTVWTWN
                     AVGKKRGAWKLGNGAPEFVDGFLLNHLIGERAADRAYANGDPVTGQAAWFDLKVRLEA
                     DPEPRVPGHRVESTV"
     gene            2792..4549
                     /locus_tag="VFK00_01420"
     CDS             2792..4549
                     /locus_tag="VFK00_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7591648.1"
                     /translation="MTWQAWATAAIILFAMVLFASEKVRVDLVALITLGLLVALGIVT
                     PDQALSGFANEATVTVAAMFALALGIERSGALEPIIRMLTRIRKPWLLSLGMMVVIAP
                     LGAFIKNTALVATFLPLSLRVCQRTNTSPARVLMPMAFAAQMGGVCTLIGTSSNLLVD
                     SLAQKQGLPAFGIFEFTPLGVMLAIAGILYLMVIGRWLLPKHIDAESPAALQVGKYVT
                     ELKLMSDSPLVGQTIAEAKLGERGVYPLELLRGDQHMWSPRTQKLSVGDVLLVRGEWE
                     KLEEFRKQAGLELNPEFTHVGGSGATNVMIEAMIAPASLAEDRTLRDLDLLPNYQATV
                     LGIHRRGHLLRDQIRDVAFRTGDVLMLLLPKEEIKRLRRDDRFVVLHERDDVRRPPHK
                     AIIAVAIMAAVVVVSALRWLPIPIAAICGVAAMALTRCFGRKDVYEGMDWKIIILLGA
                     ILPLGIAIETTGLAHVLVEGALDLVGNHGPLAALLMVYVLTALLTELMGHNPSVVLMV
                     GIAVSVAHAVHADPRPFVVAVAFAAATSFATPVGYPTNTMVYYAGGYRFLDFTKVGVP
                     LIALFCVLSMYLIPLIWPF"
     gene            4678..5604
                     /locus_tag="VFK00_01425"
     CDS             4678..5604
                     /locus_tag="VFK00_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7591649.1"
                     /translation="MTALPAPSPKKLGLVIDLDTCVGCHACAVACKEWNDGGQFGPLP
                     DDEPYGAEPHGVWFNRVHTYEVTAPGTGDLGPAALGLPGSNATNRSADATQSRESCTA
                     DAPFPDPRSPLPQMVHFPRSCLHCETPDCVTVCPTGASYKRAEDGIVLVDEDKCIGCQ
                     LCAWACPYGAREIALPPAKKARASDVGRKDSKRGTMQKCTLCVDRIYNETLDERDRQP
                     ACVMACPTRARMFGDLGDPDSDVSKVVAERNGYALMPEFGYRPVNRYLPPRPRRDGAN
                     AAAATAPAAAEETLDTTQLPPLLRWLDKALSR"
     gene            5601..6560
                     /locus_tag="VFK00_01430"
     CDS             5601..6560
                     /locus_tag="VFK00_01430"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7591650.1"
                     /translation="MRPSLSVILFTVLAGAGYGLWWLLGAALAAGIYPFDGTGILVPL
                     AFGFVLVTAGLLASTAHLGRPERAWRALSQWSSSWLSREGVASLVTYLPMLAVAWLAY
                     AHRPGWPVRVAGGWLALCALVTLVCTANIYRSLKTIRAWRMPQVLPSYLVFATLSGGL
                     WLWAWLALAPGNAIRPAFPLALLALAVIAAVIKTGYWRSIDRTPVFTAGHATGLEHLG
                     RVRSFEGPATEQTYLTREMAFVLARKHSARLRKGAVILFAILPVAGIAVALPYRAGWV
                     APVVAILCLAGLFVERWLFFAEARHAVAAYFPPQQDRTPSAAR"
     gene            6639..7478
                     /locus_tag="VFK00_01435"
     CDS             6639..7478
                     /locus_tag="VFK00_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET7591651.1"
                     /translation="MNRSRIMAVVAAGALLGLPALVWAQAPGTSNWSGTGEFGLANSA
                     GNTKSLNADAKFKLAYEDDIWKDAFFLNVNRAKSNVKSPVVVDGVVVGETSSYQTTAN
                     HFDVGGSLGYKFNPRSYMIAAARYDHDDFAPNRWQEVASIGFGYILFKNARAEFSIEA
                     GPGYKRYQPQPYIQVDASVTPPVATQIKPPAENEAIGRGLVNYKLAITDSVSLQETFL
                     VEAGSQNRYYQNDIGVAVAMTRTMALKVAYENRYNSNIVPGTEHLDSLFTTNLVYNFG
                     MTR"
     gene            complement(7574..8488)
                     /gene="hemC"
                     /locus_tag="VFK00_01440"
     CDS             complement(7574..8488)
                     /gene="hemC"
                     /locus_tag="VFK00_01440"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7591652.1"
                     /translation="MSILRIATRKSPLALWQAEHVARRLRAAHAGLEVELLPLSTRGD
                     EVLDRSLAAIGGKGLFLKELEVALAEGRADIAVHSLKDVPAILEAGFELSAILAREDC
                     ADAWISNDFASIDDLSQRARVGTSSLRRSAQLRARRADLVIADLRGNVGTRLAKLDRG
                     DYAAIVLAVAGLKRMGLESRIRARLAPPEWLPAPGQAAIAVEARAGNARVATLLAPLD
                     DAETRRAVMAERALNAAMGGDCTMPLGAWCETRADGRLHLRGLLGDARDGRLLRADAV
                     GEDPHVLGAEVAKRLREQGADALLQSRR"
     gene            8674..9153
                     /locus_tag="VFK00_01445"
     CDS             8674..9153
                     /locus_tag="VFK00_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591653.1"
                     /translation="MKKLSLLGIGMAAALAAAGCSTTPADTGGQSSVAERGSPDLDCK
                     MSFSLTGWAALYKHAEGVGTVTCENGQSLPVTISVKGGGLTAGKWHVDNGSGTFTDVH
                     SIGDVLGRYVQGGAHAGVVKSGSAQVLTKGTVSLALAGSGEGIDLGVDVGSFTISRR"
     gene            complement(9230..10540)
                     /locus_tag="VFK00_01450"
     CDS             complement(9230..10540)
                     /locus_tag="VFK00_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7591654.1"
                     /translation="MSQFRLLGQRRFAPFFAVQSLAAFNDNAFRQATIVLVGFQMGLS
                     TATIGLYSNLAPALFILPFFLFSASAGQIAEKFEKQRLIRYVKLFEIAAMCVAAWGFH
                     VRSPLLLFVVLFLMGVHSTVFGPIKYSLLPEVLEPRELVGGNGLVEMGTSLAILVGMT
                     VGGAAMHEFGAFAAAALVIAVACAGLGASHAIPRLPAVAPGLKFNWNPLTESARVLRI
                     TAKDRMVFNAVLGISWFWFFGGVVTAQLPNYTRLYLGGDASVEILVLTLFSVGIGVGS
                     LLCEKLSRHEVEVGLVPLGAAGMTVFGVLLYVLQHAGAAVRGANWLSFLGSPGRGWLA
                     LDLVLIGASAGLFIVPLFALVQSRSPREQVSRVIAGNNIINAIFLVAAALFGIGLLHA
                     GLTIPQLFLVVAVLNALVMGYLAWRVPAFARRFFRWSRMRRASA"
     gene            complement(10584..11867)
                     /gene="purD"
                     /locus_tag="VFK00_01455"
     CDS             complement(10584..11867)
                     /gene="purD"
                     /locus_tag="VFK00_01455"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7591655.1"
                     /translation="MKLLVIGSGGREHALAWKLAQSSRVSDVLVAPGNAGTAGEPRMR
                     NAPVAATDLDGLLQLARDEHVDLTVVGPEAPLVAGIVDKFRAAGLRIFGPTAAASALE
                     GSKAFAKAFLERHGIPTARHAVFTDLDAALAYVRAQGAPIVVKADGLAAGKGVVVATT
                     LPEAEDALREMLGAHAFGDAGARVVIEEYLEGEEASYIVMADGAHALPMASSQDHKRR
                     DEGDLGPNTGGMGAYSPAPVVTPDVEQRILREIIQPTLRGMAAEGAPFTGFLYAGLMV
                     DQHAAPKVIEFNVRFGDPECQPILMRLESDLADLVEAALDGKLDQAQVRWDPRPSLGV
                     VVAAQGYPANPTKGDVVSGLADDPAHGVKVFHAGTKRDAQDRVVTAGGRVLTACALGD
                     DLSQARDRAYAVVDRIRFDGAFCRRDIGHRALARR"
     gene            complement(11907..13295)
                     /locus_tag="VFK00_01460"
     CDS             complement(11907..13295)
                     /locus_tag="VFK00_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7591656.1"
                     /translation="MSSVYESPQGEPAGLCRPLLANVPAGRTIAWRGGRAVTAAAFLA
                     DVTALAERLPQGTAAVNLCEDRYAFLVAFAALASRGQVNLLPPSRVPHAIDEALAAHP
                     GSYTVGDSALVPAPPHYLRFAPQGVARALPPVLVPEATDVAIGYTSGSTGVPRANAKR
                     WDKLGVSSGHNDALLRGLAGGDYHVVATVPPQHMYGLEFSVLLPLFGYAAVYDGRPFF
                     AADIADALASLPEPRILVTTPVHLRALVDSNQALPAFSGIVSATAPLSRELALAAEDR
                     FGAPVQEVFGSTETCVIAHRRTARDEAWTTYPGVVLHPQPDGTRIEAPQLVQPVVLAD
                     IVELEAEGHFRLCGRNTDLLEIAGKRASLGDLTCKLLAVPGVRDGVVFQLDDGDAVGV
                     RRIAALAVAPGLSAAAVLDALRGSIDPVFLPRPLKLVDALPRSETGKLPRVAMVETLK
                     SGNGEPVPGKNL"
     gene            complement(13314..13883)
                     /locus_tag="VFK00_01465"
     CDS             complement(13314..13883)
                     /locus_tag="VFK00_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET7591657.1"
                     /translation="MNTRVFDRDAIEGAAALLRSGGVLAYPTEGVYGLGCDPDNRAAF
                     EKIFALKRRPPEQGVLLIAAGFDQVRDWIGDAPKAAIQRASAVWPGAHTFIFPRSPRV
                     PPWVAGGHAGIALRVTAHAPSVALCRAFGGPIVSTSANRHGEPPARSADAAARLFGDD
                     LDGVLDAPLGGLDRPTPITDAVSGAIIRA"
     gene            complement(13880..16393)
                     /locus_tag="VFK00_01470"
     CDS             complement(13880..16393)
                     /locus_tag="VFK00_01470"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET7591658.1"
                     /translation="MAKNLLIVESPAKAKTINKYLGADFKVLASYGHVRDLVPKEGAV
                     DTEHDFAMDYALIDKNIKHVDDIAKAAKAADSIYLATDLDREGEAISWHISEILKERG
                     LLEDRETHRVVFSEITPRAIKEAVAHPRRLSHDLVDAQQARRALDYLVGFNLSPVLWR
                     KVQRGLSAGRVQSPALRMIVEREEEIEAFVPREYWTVEAKLAHRDGEFTARLVKLHGK
                     KFEQFDLTNADDAHAARDALFAAAAGKLVVGDIQRKQRQRRPAAPFTTSTLQQEAARK
                     LGMATSRTMRVAQGLYEGVSLGGEGNVGLITYMRTDSTHLSGEAIGELRQAIEREFGR
                     QGLPEHAMVYKTKSKNAQEAHEAIRPTSALRTPKSVAAYLTPDQLRLYELVWKRAVAC
                     QMKPATLNTVSVEFPCGDGAFRATGTTVVDPGFLAVYEEGRDQKSAEDEESRKLPALE
                     VGESVPLTDIAADQHFTEPPPRYSEASLVKTLEEYGIGRPSTYASIIQTLLSREYVIL
                     DSRRFKPTDVGRAVAKFLTAHFTRYVDYDFTAKLEDELDAVSRGEEEWRPLMAGFWKP
                     FKALVDDKMETVDRSEATGARLLGDDPRTGKPVSVRLGRYGPYAALGDKDKDDEKLKF
                     ASLLPGQSMHTITLEEALELFKLPRKLGHDGDGEEISAGIGRFGPFVKQGKLYASIKG
                     DESAYTITLERARELIREKQELIANRVINDFGDGIQVLNGRYGPYITNGEKNAKVPKE
                     REPKSLSHEECKALLEAAPVRRGRGAFKKTAAKKAAARKAPAKKPAARKPAAKKASAK
                     KSAAKKTGRKTAARKSTARKAAGAKPAVAADADTPPFDV"
     gene            complement(16571..>17368)
                     /gene="dprA"
                     /locus_tag="VFK00_01475"
     CDS             complement(16571..>17368)
                     /gene="dprA"
                     /locus_tag="VFK00_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7591659.1"
                     /translation="VAVVGARSATAQGLATARDFARTLSHAGLTITSGMADGIDGAAH
                     AAALEAGGATIAVVGTGPDIVYPSKHRELAAKIEGSGAIVSEFAPGTEARPGHFPRRN
                     RLIAGLALGTLVVEAGLQSGSLITARLAAEAGREVFALPGSIHNPLAKGCHRLIRDGA
                     RLVETANEVVEAIVPAARALGADLRARLETEEAPAAGNGMDRSQDPDYAALLAALGDA
                     PASLDELAGRTGLAPAALSSMLLLLELEGTVAPAVHGRWQRLFAAGA"
BASE COUNT         2778 a         5992 c         5815 g         2783 t
ORIGIN      
        1 ttcggcggct cgttctggga attcggcgaa ccggactggg accgcaccaa atacctgatg
       61 ctgtggggcg tggccgagga ccacgattcg aacccgatca agctcggcct cggcaagatg
      121 aaggcgcgcg gcgccaagat cgtcgcgatc aatccggtgc gcaccggtta cggctcgatc
      181 gccgacgaat gggtgccggt gaatcccggc acggatggct tgctggcggg cgcgctgatc
      241 cacgaactgc tgcgcaacga tcgcgtcgat ttcgaatacc tcgtgcgcta caccaacgcg
      301 catcacctgg taatccgcga tccggacgcg gcggatgacg gactgattgc acgtgatgcg
      361 gatggcaaac cgctctgcgc cgcacgtgcg ggcacaccac agccggccgc tcgtcccggc
      421 gcggcacctt cggggctgcg cgccacctca tccggtggcg gggccttctc ccttttcccc
      481 ccgggagaag gtgcccgcag ggcggatgag ggtgcgacac cgccttaccg cgtcgttctc
      541 gccgatgcag ccggcatctt gccgatggtg gtgggtgaat atgccctcca cgacggtcgt
      601 cgcgcggtgc ctgcgttcca gctcctcgcc gagcgttttc tcgcacaaga gtacgcgccg
      661 gagaacgtca gcaaacgctg cggcgtgccg gcctcgacca tccgccgcct cgcggccgag
      721 cttgcgcatg tcgcgttcga cgaggcgatc gagctgccga tagcctggac cgacacccac
      781 ggcagaaggc acgccgcgat ggtcggccgg cccgtgtcga tgcacgccat gcgcggcatc
      841 gccgcccacg ccaacggctt ccatacctgc cgcacgatcc acgtgctgca gatgctgctg
      901 ggcgccatcg acacgcccgg cagcttccgt taccagccgc cgtacccgaa accggcgccg
      961 cccggcaaca aaccggccag ggcgcgcaaa ccggacggta cgctgacggg caatccgctc
     1021 ggctatcccc agtcgccgga cgacctgctg gtcgatgccg aaggcaagcc gcgccggatc
     1081 gaccacgcct tcagttggga atacccgctg gccgcgcacg gcctgctgca gtcggtaatc
     1141 cgcaacgcgg tcgcaggcga tcccgcgaag atcgacacgc tgttcctgtt catggcgaac
     1201 atgggctgga attcggcgat ggacacggcg aatacgcgca agctgctttg cgagcgtggc
     1261 gccgacggcg aatacaagat cccccgcatc atctacgccg atgcctacga ttccgagacc
     1321 gtcgcgttcg ccgacctcgt gctgcccgac accacgtatc tcgagcgcta cgactgcatg
     1381 agcctgctcg atcgtccgat cagcgatgcc gaatcggcgg ccgacgcgat ccgccggccg
     1441 gtggtcgcgc ccgatcgcga cgtgcgtccg ttccaggacg tgctgctgga gctcggtgcg
     1501 cgcctgaagc tgccgaacat ggcggattcg gacggcacat ccgtgtacca ggattacgcc
     1561 gactacatgg tgcgccacga acgcgctccc ggcgtcggca tgctcacggg ctggcgtggc
     1621 gccgacggga tgcaggccgg caagggcgcg gccaatccag accagttgca gcgctacatc
     1681 gagcacggct gcttcttcga agcacgcgtg ccggaagcag gcgcgtacta ccggatggcg
     1741 aaccgcgact acctcgcatg ggcgcagtcg atgggcttcg tcgcctcgac cgaaccggtc
     1801 acgatgcagt tgtattgcga ggtgctgcag cgcttccggc tggcggcaca ggggcacggc
     1861 gccgtgcagc cgccggaacg ggagcgtgcg cgcgtcgcgc ggtatttcga tccggtcccc
     1921 atgtggtact cgtcgcaaag cgacactgat tcccctctcc cttcgggaga gggtgacaag
     1981 cggcgtgtgc gagatgccga tggcatctcg cgccgcttcg aacgggcggc caaggatggc
     2041 cgcagcggcg ctccgcacca tggagggttg cttgccgaat tcgaagcttc ccccggcgct
     2101 tcgcacagac gaaaaggcgg agcgcccctt ctcccggagg gagcagggaa agccgagttt
     2161 cccttgtccg ccatcagcca gcggccgatg ttcatgtacc acgcctgggg ttcgcagaac
     2221 cgctggctgc ggcagatcgc cgcgcgcaac gtgctctacg tgcatcccga cgcgctggcc
     2281 gcggcggcca tcgccgatgg cgactgggcc tggatcgcct cgccccgcgg acgcctgcgc
     2341 gttcaggtgc gcgcgcacgc cggctgcgcg cccggcacgg tgtggacctg gaacgccgtc
     2401 ggcaagaagc gcggcgcctg gaaactcggc aatggggcgc cggaattcgt ggatggcttc
     2461 ctgttgaacc acctgatcgg cgaacgcgcg gccgaccgcg cttacgccaa cggcgacccg
     2521 gtcaccgggc aggccgcatg gttcgacctc aaggtaaggc tggaggcgga ccccgaaccg
     2581 cgagtaccgg ggcatcgggt ggaatccacc gtctgaacga tttttttgca ggagcgtggc
     2641 aagctccgat ggacgccgga ccggctgcgg gtgcggacgc cggggcgaac acggcaaaat
     2701 gggcattcac gggcatcaca gggtggcggg cacgatgctg gtttggggag agcaggttgg
     2761 ctgaacacac gttcgaatcg aggcctggcg catgacctgg caggcctggg ccactgcggc
     2821 catcatcttg ttcgcgatgg tgctgttcgc cagcgagaag gtccgcgtgg acctcgtcgc
     2881 actgatcacg ctcggccttc tggttgcgct cggcatcgtc acgccggacc aggcgttgtc
     2941 gggcttcgcc aacgaggcca ccgtgacggt ggcggcgatg ttcgcgctcg cgctcgggat
     3001 cgagcgctcc ggcgcgctcg aacccatcat ccgcatgctc acgcggatac ggaaaccgtg
     3061 gctgttgtcg ctcggcatga tggtggtgat cgcaccgctc ggcgcgttca tcaagaacac
     3121 cgcgttggtt gccaccttcc tgccgctgtc cttgcgggtc tgccagcgca ccaacacttc
     3181 accggcgcgc gtcctgatgc cgatggcctt cgccgcccag atgggcggcg tgtgcaccct
     3241 gatcggcacg tcctcgaacc tgctggtcga ttcgctggcg caaaagcagg gactcccggc
     3301 cttcggcatt ttcgaattca cgccactcgg cgtcatgctg gccatcgccg gcatcctgta
     3361 cctgatggtg atcgggcgat ggctgctgcc caagcacatc gatgccgaat cgccggccgc
     3421 gctgcaggtc ggcaagtacg tcaccgaact caagctgatg tccgattcgc cgttggtcgg
     3481 gcagaccatc gccgaggcga agctcggcga gcgcggcgtg tatccgctcg aactgctgcg
     3541 cggcgaccag cacatgtggt caccgcggac ccagaagctc agcgtcggcg acgtgctgct
     3601 ggtccgcggc gagtgggaaa agctggagga gttccgcaag caggcgggcc tggaactcaa
     3661 ccccgagttc acccacgtcg gaggcagcgg cgcgaccaac gtgatgatcg aggcgatgat
     3721 cgcgccggcc agccttgccg aggaccgcac cctgcgcgac ctcgacctgc tgcccaatta
     3781 ccaggccacc gtgctcggca tccatcgccg cggccacctg ctgcgcgacc agatccgcga
     3841 cgtggcgttc cgcaccggcg acgtcctgat gctgttgttg cccaaggagg agatcaagcg
     3901 cctgcgccgc gacgaccgct tcgtcgtcct gcacgaacgc gacgacgtcc gccggccccc
     3961 gcacaaggcg atcatcgcgg tggcgatcat ggccgcggtg gtcgtggttt cggcgctgcg
     4021 ctggctgccg atcccgatcg ccgccatctg cggcgtcgcg gccatggcgc tgacgcggtg
     4081 ctttgggcgc aaggacgtgt acgagggcat ggactggaag atcatcatcc tgctcggcgc
     4141 gatcctgccg ctcgggattg cgatcgagac cacgggcctt gcccatgtgc tggtcgaagg
     4201 cgcgctcgac ctggtcggca accacggccc gctcgccgcg ctgctcatgg tatacgtcct
     4261 caccgcgctc ctcaccgagt tgatggggca caacccttcg gtggtgctga tggtcgggat
     4321 tgcggtcagc gttgcccatg ccgtgcacgc ggacccgcga ccgttcgtgg tggcggtggc
     4381 gttcgccgcc gcaacctcgt tcgccacccc cgtcggatat cccaccaaca cgatggtgta
     4441 ttacgctggc ggttatcgat tcctcgattt cacgaaggtc ggcgtgccgc tgatcgcgct
     4501 gttctgcgtg ctgtcgatgt acctgattcc gctgatctgg ccgttttgac cgtccgtgct
     4561 gtcttgtggc ccgacattcc ccttccggtt ccacgcgacg cctgacgcgc ggagggcctt
     4621 ccttccgggc ggcgcgttcg ccgcaacggc aggcaaatca cgcacggaaa cgcgccgatg
     4681 accgccctgc ccgcgccatc cccgaaaaag ctcgggctgg tgatcgacct cgacacctgc
     4741 gtcggctgcc acgcctgcgc ggtcgcctgc aaggaatgga acgacggcgg ccagttcggt
     4801 ccactgcccg atgacgagcc ctacggcgcc gagccgcacg gggtgtggtt caaccgggtg
     4861 catacgtacg aggtgacggc accggggacc ggggacctgg gcccggccgc gctcggactt
     4921 cccggttcaa atgccaccaa ccgaagcgcg gatgcgacgc aaagcaggga atcatgcact
     4981 gcggatgctc cgttccccga tccccggtcc ccgctcccgc aaatggtcca cttcccgcgc
     5041 tcgtgcctgc attgcgagac gccggattgc gtgacggttt gcccgaccgg tgcgagctac
     5101 aagcgcgccg aggacggcat cgtgctggtc gacgaggaca agtgcatcgg ctgccagttg
     5161 tgcgcatggg cctgcccgta tggcgcgcgc gagatcgcgt tgccgccggc gaaaaaagcc
     5221 cgcgcttccg atgttggccg caaggacagc aagcgcggca cgatgcagaa gtgcacgctg
     5281 tgcgtggacc gcatctacaa cgaaaccctc gacgaacgcg accggcaacc ggcctgcgtg
     5341 atggcctgcc ccacgcgtgc gcggatgttc ggcgatctcg gcgatccgga ttccgacgtg
     5401 tccaaggtcg tcgccgaacg aaacggctac gcgctgatgc cggagttcgg ctatcggccg
     5461 gtgaaccgtt acctgccgcc gcggccgcgc cgcgatggag caaacgccgc ggccgccacc
     5521 gcgccggcgg cggcggaaga aaccctggac accacgcaat tgccgccctt gctgcgctgg
     5581 ctggacaagg cgctgtcgcg atgaggcctt cgctctcggt gatcctgttc acggtgctgg
     5641 ccggcgcggg ctatggcctg tggtggctgc tcggcgccgc gctggccgct ggcatctacc
     5701 cgttcgatgg cactggaatt ctggtgccgc tggcgttcgg tttcgtgctc gtgaccgcgg
     5761 gattgctcgc ctcgaccgcg cacctcggcc gtccggagcg cgcctggcgt gcgctcagcc
     5821 agtggagcag ctcctggctg tcccgcgaag gcgtcgcatc gctcgtgacc tacctgccga
     5881 tgctggccgt cgcgtggctc gcatacgcgc accggccggg ctggcccgtc agggtcgccg
     5941 ggggatggct cgcgctatgc gcgcttgtga ccctcgtgtg caccgccaac atctaccggt
     6001 cgctgaagac catccgcgcc tggcgcatgc cgcaggtgct gccgtcgtac ctggtgttcg
     6061 cgacgctttc cggcggactg tggctgtggg cgtggctggc actcgcgccg ggaaacgcga
     6121 ttcgcccggc cttcccgctc gccctgctgg ccctggcggt gatcgccgcg gtgatcaaga
     6181 ccggatactg gcggagcatc gatcgcacgc cggtattcac ggctggacac gcgaccggcc
     6241 tcgagcacct gggacgggtg cgctcattcg aagggccggc taccgaacag acttacctca
     6301 cccgcgagat ggcgttcgta ctcgcgcgaa aacactccgc gcgcctccgc aagggcgcgg
     6361 tgatcctgtt cgcgatcctg cccgtggccg gcatcgccgt ggcgctgccg tatcgcgccg
     6421 gatgggtcgc accggtggtg gcgatcctct gcctcgcggg attgttcgtc gaacgctggc
     6481 tgttcttcgc cgaagcgcgc cacgcggttg ccgcgtactt tccgccgcag caggatcgaa
     6541 ccccatccgc tgcccgctga tcacgaaaat ttaacaggcg ttcagcgccg ccgatgcagc
     6601 ctttcgggtt ggcattcggc aacatcaagg agttgcacat gaaccgcagc cgcatcatgg
     6661 ccgtggtggc ggcaggcgcc ctcctcggcc tgccggcgct ggtatgggcg caggcgcccg
     6721 gaacctccaa ctggagcggc accggcgagt tcggcctcgc caactccgcc ggcaacacca
     6781 aatcgctgaa cgcggacgcc aagttcaagc tcgcctacga ggacgacatc tggaaggacg
     6841 cgttcttcct caacgtcaac cgtgccaaga gcaacgtgaa gtctccggtg gtggtcgacg
     6901 gcgtggttgt cggggaaacc agcagctacc agacgacggc caatcatttc gacgtgggcg
     6961 gatcgctcgg ctacaagttc aacccgcgtt cctacatgat cgctgcagcg cgttacgacc
     7021 acgacgattt cgcacccaat cgctggcagg aggtggcgtc gatcggcttc ggctacatcc
     7081 tgttcaagaa cgcccgcgcg gagttttcga tcgaagccgg ccctggctac aagcgctacc
     7141 aaccgcagcc ctacatccaa gtcgatgcca gcgtgacgcc accggtggcc acgcagatca
     7201 agcctccggc cgagaacgag gccatcggcc gcgggctcgt caactacaag ctcgcgatca
     7261 ccgacagcgt cagcctgcag gaaaccttcc tcgtcgaggc cggcagccag aaccgctact
     7321 accagaacga catcggcgtg gcggtggcca tgaccaggac catggcgctg aaggtcgcct
     7381 acgagaaccg ctacaactcc aacatcgtgc ctggcaccga acacctggac agcttgttca
     7441 ccaccaacct ggtgtacaac ttcgggatga cgcggtagtt cgggactcgg gagcgcgggg
     7501 aggctcaccc ccttccgagg gaagggcggc ccgcaggagc gggcagcgct gccgcgcagc
     7561 agcggggaat gctctatcgg cgggattgca gcagtgcgtc cgcgccctgc tcgcgcaggc
     7621 gctttgcgac ttcggcaccg aggacatggg gatcctcgcc aaccgcatcg gcgcgcagca
     7681 aacgtccgtc ccgggcgtcg cccagcaatc cgcggaggtg caacctgccg tccgcacgcg
     7741 tttcgcacca cgcgcccaac ggcattgtgc agtcgccgcc catcgccgca ttgagcgctc
     7801 gctcggccat gacggcgcgg cgggtctccg cgtcgtccag cggcgccagc agcgtggcga
     7861 cccgggcgtt tccggcgcgc gcctccactg cgatcgccgc ttgcccgggc gcaggcagcc
     7921 attcgggggg ggccagccgc gcgcggatgc gtgactccag tcccatccgc ttcaggcccg
     7981 ccaccgcgag cacgatcgcc gcgtaatcac cccgatcgag tttggccagc cgcgtgccca
     8041 cgttgccgcg caggtcggcg atgacgaggt cggcacgccg ggcccgcaac tgtgcgctgc
     8101 gccggagcga cgaagtgccg acgcgggcgc gctgcgacag atcgtcgatc gacgcgaaat
     8161 cgttgctgat ccacgcgtcg gcgcagtctt cgcgagcgag gatcgcgctc agctcgaatc
     8221 ccgcctcgag gatcgccggt acgtccttca gcgaatgcac cgcgatgtcc gcgcgtccct
     8281 cggccagcgc aacctccagt tccttgagga acaacccctt gccgccgatc gccgccagcg
     8341 agcggtccag cacctcgtcg ccccgcgtcg acaagggcag caactcgacc tcgagtcccg
     8401 cgtgcgccgc gcgcagccgc cgcgcgacgt gctcggcctg ccagagcgcg agcggactct
     8461 tgcgggtggc gatgcgaagg atggacatgc gtgcaagtat cgcatcgatc gcgacgcggc
     8521 ccgaaaaatc aaccgcctgg cgcgatgccg ggccgaaagc ggcgccgttg ttcggctgcc
     8581 attcgggttg ctgaacatcg ccgccgcgct tacccgggcg ttcagttcga tccgcgaaac
     8641 tgaatgcgca agtccaaacg taaggagacg gacatgaaaa agctttccct gctgggcatc
     8701 ggcatggcgg cggcgctggc cgcggccgga tgctccacga cgccggccga caccggcggc
     8761 cagtccagcg tcgccgaacg cggctcgccc gacctcgact gcaagatgag cttttcactg
     8821 acgggatggg cggcactgta caagcatgcc gaaggcgtcg gcacggtcac ctgcgagaac
     8881 gggcagtcgc tgcccgtcac catttcggtc aagggtggcg gcctgaccgc cggcaagtgg
     8941 cacgtcgata acggctcggg cacgttcacc gatgtgcatt cgatcggtga cgtgctcggc
     9001 cgctacgtgc aaggcggcgc gcacgccggc gtcgtcaagt ccggctcggc gcaggtgctg
     9061 accaagggca ccgtctcgct ggcgctggcc ggcagcggcg agggcatcga cctcggcgtc
     9121 gatgtcggct cgttcaccat cagccgtcgc taagcgtggc tgtcatggaa ggcgggcagc
     9181 ggaaaaccgc tgcccgcgtc catgcccgga gcgcgcaacc ccggcggcct cacgccgacg
     9241 cgcggcgcat ccggctccag cggaagaagc gacgggcgaa tgccggcacc cgccacgcga
     9301 gataacccat caccaacgca ttcagcaccg caaccaccag gaacagttgc ggaatcgtga
     9361 gacccgcgtg cagcaacccg atgccgaaca gcgcagcggc gacgaggaag atcgcattga
     9421 tgatgttgtt gccggctatg acccgcgaaa cttgctcgcg ggggctgcgc gattgcacca
     9481 gggcgaacag cggcacgatg aacaggcccg ccgacgcccc gatcagcacc aggtccagcg
     9541 ccagccagcc gcgtccgggc gaaccgagga acgacagcca gttcgcgccg cggacggcgg
     9601 ctccggcgtg ctgcaggacg tacagcagca cgccgaacac ggtcatcccg gccgcgccca
     9661 gcggcaccag gccgacctcg acctcgtggc gcgacagttt ctcacacagc aacgacccga
     9721 cgccgatccc gaccgagaac aacgtcagca ccaggatctc cacgctggcg tcgccgccga
     9781 gatagaggcg cgtgtagttc ggaagttgcg cggtcaccac gccgccgaaa aaccagaacc
     9841 acgagatccc cagcaccgcg ttgaagacca tgcggtcctt ggcggtgatc ctcagcaccc
     9901 gcgcgctttc cgtgagggga ttccagttga acttcaggcc tggcgcgacg gccggcagac
     9961 gcgggatcgc atggctcgcg ccaaggcccg cgcatgcgac cgcgatcacc agggccgccg
    10021 cggcgaacgc accgaactcg tgcatcgccg caccgcccac ggtcatcccg acaaggatcg
    10081 ccagcgacgt gcccatctcg accagcccgt tgccaccgac cagttcgcgc ggctccagca
    10141 cctcggggag cagcgaatac ttgatcgggc cgaacaccgt cgaatgcacg cccatcagga
    10201 acagcaccac gaacagcagc aacgggctgc gtacgtggaa gccccacgcg gccacgcaca
    10261 tcgcggcgat ctcgaacaac ttcacgtaac ggatcaaccg ctgcttctcg aacttctccg
    10321 cgatctgccc ggcgctggcg gaaaacagga agaacggcag gatgaacagc gccggcgcga
    10381 ggttggaata cagcccgatc gtcgcggtgg atagccccat ctgaaatccc accagcacga
    10441 tcgtcgcctg ccggaaggcg ttgtcgttga acgccgcgag cgattgcacc gcgaagaacg
    10501 gcgcgaaacg gcgctggccg agcaggcgga actggctcat gcgcggatgc tagcggatac
    10561 ccggcgccgg gagcggatgg acgtcagcgt cgtgccaatg ccctatgccc gatgtcgcgt
    10621 cggcagaacg ctccatcgaa ccggatgcga tccacgacgg cataggcacg gtcgcgggcc
    10681 tgcgacaaat cgtcgccgag cgcgcaggcc gtgaggaccc gaccgccggc agtgacgacg
    10741 cggtcctgcg catcacgctt cgtccccgcg tggaacacct tcacgccatg cgcgggatcg
    10801 tccgccaggc ctgaaactac gtcgcccttc gtcgggttcg cgggataacc ctgcgccgcg
    10861 accacgacgc ccagcgaagg gcgcggatcc caccgcactt gcgcctggtc gagcttgcca
    10921 tcgagcgcgg cctcgaccaa gtcggccagg tccgattcga ggcgcatcag gatcggctgg
    10981 cattccgggt cgccgaagcg cacgttgaac tcgatcacct tcggcgcggc gtgctggtcg
    11041 accatcagcc ctgcatacag gaagcccgtg aagggcgcgc cttcggcggc catgccgcgc
    11101 agcgtcggct ggatgatctc gcgcaggatc cgctgttcga cgtcgggcgt caccaccggc
    11161 gcgggtgaat acgcgcccat gccccccgtg ttggggccga ggtcgccttc atcgcgccgc
    11221 ttgtggtcct ggctggacgc catcggcaac gcatgcgcac catcggccat cacgatgtag
    11281 ctggcttcct cgccctcgag gtattcctcg atcacgaccc gggcaccggc gtcaccgaag
    11341 gcgtgcgcgc cgagcatctc gcgcaacgcg tcctcggcct ccggcagcgt cgtcgcaaca
    11401 accacgccct tgcccgcggc aaggccatcc gccttgacca cgatcggcgc gccctgggca
    11461 cgcacgtatg cgagcgcggc atccaggtcg gtgaacaccg cgtggcgcgc ggtcgggatg
    11521 ccgtggcgtt cgaggaatgc cttggcgaag gccttggaac cttccagcgc cgacgcggcg
    11581 gcggtcggtc cgaagatccg caggcctgcc gcccggaact tgtccacgat cccggcgacc
    11641 agcggcgctt cgggaccgac gacggtgagg tcgacgtgct cgtcgcgcgc gagttgcagc
    11701 aagccatcga ggtcggtcgc ggccaccggg gcgttgcgca tgcgcggctc gcctgcggtg
    11761 cccgcattgc cgggcgcaac cagcacgtcg gagacgcgcg atgactgcgc gagtttccag
    11821 gccaacgcgt gctcgcggcc gccggagccg atgacgagaa gcttcatgga gcgggaatgg
    11881 ggaacagaga acggggatgt gggaggttac aggtttttcc cgggtaccgg ttccccgttc
    11941 ccgctcttca acgtttcaac catcgccacg cgcgggagct tgccggtttc gctgcgcggc
    12001 agcgcgtcca cgagcttcag cgggcgcggc aggaacaccg ggtcgatgct gccgcgcagc
    12061 gcgtcgagga ccgcggccgc cgaaaggcca ggcgcgaccg caagcgcggc gatccgtcgc
    12121 acgcccaccg cgtcaccgtc gtcgagctgg aacacgaccc cgtcccgcac gcccggcacc
    12181 gccagcagct tgcaggtgag gtcgcccagc gacgcgcgct tgccggcgat ttccaggaga
    12241 tcggtgttgc ggccgcacaa gcggaaatga ccttccgctt ccaattccac gatatcggca
    12301 agcaccaccg gctgcacgag ctggggcgcc tcgatgcgcg tgccatcggg ctgggggtgc
    12361 aggaccacgc ccggatacgt tgtccacgct tcgtcccgcg ccgtgcggcg gtgcgcgatc
    12421 acgcaggtct ccgtggaacc gaacacttcc tgcaccgggg ccccgaagcg atcctcggcc
    12481 gcgagcgcaa gttcgcggga cagtggcgcc gtcgcggaca cgatgccgga gaacgccggc
    12541 agcgcctggt tggaatcgac cagtgcacga agatgcaccg gcgtcgtgac caggatgcgc
    12601 ggttcgggca aagaagccag cgcgtcggcg atgtcggccg cgaagaacgg gcggccgtca
    12661 tacaccgcgg catagccgaa cagcggcagc agcaccgaga actccaggcc gtacatgtgc
    12721 tgcggcggca ccgtcgcgac cacgtggtag tcgccgccgg ccaacccgcg cagcagcgca
    12781 tcgttgtggc cgctcgatac gccgagcttg tcccagcgct tggcgttggc gcgcggcacg
    12841 ccggtggatc cggacgtgta gccgatcgcg acatccgtag cctccgggac cagcaccggc
    12901 ggcaacgcac gcgccacgcc ctgcggcgcg aagcggaggt aatgcggcgg tgccgggacg
    12961 agtgccgaat cgccgaccgt gtagctgccg ggatgcgcgg ccagtgcctc gtcgatcgca
    13021 tgcggcacgc gcgaaggcgg cagcaggttg acctggccgc gcgaggccag cgcggcaaat
    13081 gcgaccagga acgcgtaacg gtcttcgcaa aggttcacgg cggccgtgcc ttggggcagc
    13141 cgctcggcga gcgccgtgac atccgcgaga aacgccgccg ccgtgaccgc gcggccgccg
    13201 cgccacgcaa tcgttcgccc tgccgggacg ttcgcgagca acggccggca cagtccggca
    13261 ggctcgccct gtggtgattc gtagacgctg gacaccgtga tttccccgtt ccctcaggcg
    13321 cggatgatag cgccgctgac ggcatcggtg atcggcgtgg gccgatcgag gccgcccagc
    13381 ggggcgtcca gcacgccgtc gaggtcgtcg ccgaacaggc gcgcggcggc gtccgcggaa
    13441 cgggcgggcg gctcgccatg gcgattcgcg ctggtggaga cgatcggtcc gccgaaggcg
    13501 cggcacagcg cgactgaggg cgcatgcgcg gtcacgcgca gcgcgatccc ggcgtggccg
    13561 ccggccaccc agggcggcac ccgtggggaa cgcgggaaga tgaaagtatg cgcgccgggc
    13621 cacacggcgc ttgcgcgctg gatcgcggcc ttcggtgcat cgccgatcca gtcgcggacc
    13681 tggtcgaagc cggccgcgat cagcagcacg ccctgctccg gcggccggcg cttcaacgcg
    13741 aagattttct cgaaggcggc tcgattgtcc ggatcgcacc ccaggccgta gacgccttcg
    13801 gtcgggtacg cgagcacgcc accggaacgc agcagggcgg ctgcgccttc gatggcatcg
    13861 cgatcgaaga cgcgcgtgtt cacacgtcga acggcggggt atccgcgtcg gccgccaccg
    13921 cgggcttggc gcccgccgcc tttctggcgg tggacttccg ggccgcggtt ttcctgccgg
    13981 ttttcttcgc cgcggatttc ttcgcggatg ccttcttcgc ggcgggtttc ctggccgcag
    14041 gcttcttcgc gggcgccttc ctggcggcgg ccttcttcgc cgccgttttc ttgaacgcgc
    14101 cgcgcccgcg acggaccggt gccgcttcga gcagcgcctt gcattcctcg tggctcaagg
    14161 atttcggctc ccgctccttg ggcaccttcg cgttcttctc gccgttggtg atgtacgggc
    14221 cgtagcgccc gttcaacacc tggatgccgt cgccgaaatc gttgatcacg cgattggcga
    14281 tcagctcctg cttctcgcgg atcagttcgc gcgcgcgttc cagcgtgatc gtgtacgcgc
    14341 tttcgtcgcc cttgatcgac gcgtacagct tgccctgctt gacgaagggt ccgaaccggc
    14401 cgatgccggc gctgatttcc tcgccatcgc catcgtgccc gagcttgcgc ggcagcttga
    14461 agagttccag cgcctcttcg agcgttatgg tgtgcatgct ctgcccgggc aggagcgacg
    14521 cgaacttcag tttctcgtcg tccttgtcct tgtcgccgag cgcggcgtag ggcccgtagc
    14581 ggccgagccg caccgagacc ggcttgccgg tcctgggatc gtcgcccagg aggcgcgcgc
    14641 cggtagcctc gctgcgatcg acggtttcca tcttgtcgtc gaccagcgcc ttgaacggtt
    14701 tccagaaccc ggccatcagc gggcgccatt cctcctcgcc gcggctgacc gcgtcgagct
    14761 cgtcttccag tttcgcggtg aagtcgtagt ccacgtatcg ggtgaagtgc gcggtcagga
    14821 acttggcaac cgcgcggccc acgtcggtgg gcttgaagcg gcgcgaatcc aggatcacgt
    14881 actcgcgcga cagcagggtc tggatgatgc tggcgtaggt ggacggacgg ccgatgccgt
    14941 attcctcgag cgtcttgacc agcgacgcct cggagtagcg cggcggcggc tcggtgaaat
    15001 gctggtcggc cgcgatgtcg gtgagcggca cggattcgcc gacctcgagc gccggcagct
    15061 tgcgcgattc ctcgtcctcg gccgacttct ggtcgcggcc ttcctcgtac accgcgagaa
    15121 acccggggtc gaccacggtg gtgccggtgg cgcggaacgc gccatcgccg cacgggaatt
    15181 cgaccgacac cgtgttcagg gtcgccggct tcatctggca ggcgaccgcg cgcttccaga
    15241 ccaattcgta gagcctcaac tggtcggggg tcaaatacgc cgcgacggat ttcggcgtgc
    15301 gcagcgccga ggtgggccgg atcgcctcgt gcgcttcctg cgcgttcttg gacttggtct
    15361 tgtagaccat cgcgtgctcg ggcagcccct gcctgccgaa ctcgcgctcg atggcctgcc
    15421 gcaattcgcc gatcgcttcg cccgagaggt gcgtcgagtc ggtacgcatg taggtgatca
    15481 ggccgacgtt gccctcgccg ccgagcgaca cgccttcgta cagcccctgc gcgacgcgca
    15541 tggtgcgcga ggtcgccatg ccgagcttgc gggcggcttc ctgctgcagc gtggaggtgg
    15601 tgaacggcgc ggcgggacgg cgctggcgtt gcttgcgctg gatgtcgccg acgacgagct
    15661 tgccggcggc ggctgcaaac agcgcgtcgc gcgcggcgtg cgcatcatcc gcgttggtga
    15721 ggtcgaactg ctcgaacttc ttgccgtgga gtttcaccag gcgcgccgtg aattcgccat
    15781 cgcggtgcgc gagcttcgcc tcgaccgtcc agtattcgcg cggcacgaag gcttcgatct
    15841 cctcctcgcg ctcgacgatc atccggagcg cgggcgactg cacccggccg gcagagaggc
    15901 cgcgctggac cttgcgccac agcaccggcg acaaattgaa accgaccagg tagtccagcg
    15961 cgcgccgcgc ctgctgggca tcgaccaggt cgtgcgagag ccggcgcgga tgggccacgg
    16021 cttccttgat cgcgcgtggg gtgatttcgg agaacaccac gcgatgggtt tcgcgatcct
    16081 cgagcaggcc gcgctccttc aggatctcgc tgatgtgcca ggagatcgct tcgccctccc
    16141 ggtccaggtc ggtcgccagg tagatcgaat cggccgcctt ggcggccttg gcgatgtcat
    16201 cgacgtgctt gatgttcttg tcgatcagcg cgtagtccat cgcgaagtcg tgctcggtgt
    16261 cgaccgcgcc ctccttgggc accaggtcgc gaacgtgccc gtacgacgcc agcaccttga
    16321 agtcggcgcc gaggtacttg ttgatggtct tggccttggc gggcgattcg acgatgagga
    16381 ggttcttggc catgtcttcg agatggggtg tccgggaatt gggttcgagc cccctcgcat
    16441 cgaggaggca gcggatcgcc gcggcgaagt ccgctgtcgg gatgggtatt cggctttggc
    16501 tcgggatggg aactcgctgc ggcttccatg cccccctata tatagtgaac acatgcgcgg
    16561 ggtgcctctg tcaagcaccg gccgcgaaaa gcctctgcca gcgcccatgg acggccgggg
    16621 cgacggtgcc ttccaattcc agcagcaaca gcatcgaaga cagcgccgcg ggcgccaagc
    16681 cggtgcgccc ggcgagttcg tccagggatg cgggcgcatc gcccagcgcc gcaagaagcg
    16741 ctgcgtaatc agggtcttgt gacctgtcca tgccgttccc cgcggctgga gcctcctccg
    16801 tctccagccg cgcgcgaagg tcggcgccca gcgcgcgggc ggccggcacg atcgcctcca
    16861 cgacctcgtt ggcggtctcg accagccgtg ccccgtcccg gatcagccga tggcagccct
    16921 tggccagcgg gttgtggatc gaaccgggca gcgcgaacac ttcgcggccg gcctcggccg
    16981 cgagccgcgc cgtgatcaac gagccggact gcagcccggc ctccaccacc agtgttccca
    17041 gcgccagccc cgcgatcagg cggttgcggc gcgggaaatg tcccggccga gcttccgtgc
    17101 cgggcgcaaa ttcactgacg atcgcgcctg atccctcgat tttggccgcc agttcgcggt
    17161 gctttgatgg gtaaacgatg tcggggccgg tcccgaccac ggcgatcgtc gcgcctccgg
    17221 cttccagcgc cgccgcgtgc gccgcgccgt cgatcccgtc ggccatgccg ctggtgatcg
    17281 tgaggccggc gtggctcagg gtgcgcgcaa aatcgcgggc cgtggcgagc ccctgcgcgg
    17341 tcgcgctgcg cgcgccgacg accgccac
//
LOCUS       DASSNH010000040         9172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1600770, whole genome shotgun sequence.
ACCESSION   DASSNH010000040 DASSNH010000000
VERSION     DASSNH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600770"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /gene="rnpB"
                     /locus_tag="VFK00_01480"
     ncRNA           complement(<1..198)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFK00_01480"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(405..1271)
                     /gene="rsmI"
                     /locus_tag="VFK00_01485"
     CDS             complement(405..1271)
                     /gene="rsmI"
                     /locus_tag="VFK00_01485"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7591660.1"
                     /translation="MNANRAGTLWVVATPIGNLGDLSDRAREVLRSVAVVAAEDTRHT
                     RPLLKHAGIATPLAAVHEHNERAAVPALLARLQAGDDVALVSDAGTPLISDPGFRLVR
                     AARGIGLRVSPVPGACAAIAALSVAGLPSDRFVFVGFLPSKPPARRARLRELAGEVRT
                     LVFYESSHRIAECCADLAEAFGAEREAALLREITKLHETHLGMTLGEIAARVADDREQ
                     RLGEFVLVVAGRGEDAEAKLAEGQRVYALLRAELPPAQAAKLAAAISGAPRKALYGDR
                     GPRTGAREPGDV"
     gene            1362..3164
                     /locus_tag="VFK00_01490"
     CDS             1362..3164
                     /locus_tag="VFK00_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HET7591661.1"
                     /translation="MHRIDHFAYAVLLVAALLLTGGCATVGPSAPAASPQAQAEYTHA
                     QELYRSGNFQDAARAFLAAANSDPTIRDRATLAAAASYRSLGQIGQTAALLRRIDPHG
                     LSPADDARYRVLSAEVALQGGHPDTALKQLASLPADLPPEIEQHALDVQVRAHLATGD
                     RLGAARALVQRNASLPPAAQAGNHQQVVSILAGMGSDRLTPLYASLADSDPLKPFVGQ
                     ALRQSGKAMPRALPQPNQPVGTLTATSMPPQGYSMPSTVALLLPATGRVATAGAAVRD
                     GFFTAYFDTPVTREQRPVVKVYDTGGTADGAATAYQQAVADGAELVVGPLARSAVAAV
                     FAQGALPVPVLALNHAQGDVPTPARSIEFSLLPEADGAQLAARMITLGLHASIVFRGI
                     DDTASRTFDAFKTQFESLGGVIANAIVLQPGDVDFADQIEAALAGSGSETGIVVLLRP
                     EQARLLLPQLRLARSTLPVFATSMAYDGTEDATANVDLDGVQFCDEPWLFDAQAGLPD
                     HAMLASLLTTAGGPAARLFGFGMDAYALLPYLGWLRTHPGSYVAGATGQLTMDTFGHV
                     QRTPIWVQFQDGIARPIAAGLEAQPAAPDIPTEP"
     gene            3161..3523
                     /locus_tag="VFK00_01495"
     CDS             3161..3523
                     /locus_tag="VFK00_01495"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7591662.1"
                     /translation="MTPGGVGARFEQRALDALERAGLELVERNWRTRFGELDLVMRDG
                     DTLVFVEVRYRQHARFGGGAASVGPAKRGKLTRAAQGFLQAHPRLAAMPCRFDVVAFD
                     GHADAPSHAWHRAAFEIC"
     gene            3537..4082
                     /locus_tag="VFK00_01500"
     CDS             3537..4082
                     /locus_tag="VFK00_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7591663.1"
                     /translation="MRKTILLTTALALVCLVSACSVFTPRRGFNRVINDRNVQITAQR
                     ALDNDRELDGRAHIGTNVYNGVLLLIGEAGTEEIKQRAEADVSGYEGVRRVVNLVDVM
                     PLQSVGRSALDASLTARVKTALLHVDLPGFDPGRVKISTAHGNVYLMGLVSHQEADAV
                     VDVARNVAGVDKVVQVFEYTD"
     gene            4229..5716
                     /locus_tag="VFK00_01505"
     CDS             4229..5716
                     /locus_tag="VFK00_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7591664.1"
                     /translation="MSLPAAFVDEIREIFPADALALDEGTRAAYGYDNSRREALPDAV
                     VFPASHDQVAALVVACRRHRMPLTARGRGTNTTGATVPIAGGIVASFERMNRIVRIEP
                     GDRLAVVEPGVLNADLQTALAAHGLFWPPDPTSAPYCTIGGNLACNAGGPHTVKYGAS
                     RDNILGLRAVAGNGTSFRSGTHTTKGATGYDLTRIIVGSEGTLALITEATLKLTPKPT
                     AVRTLRAAYDGIQAAADAVARIMAQPVTPRALEFMDEAALQLVRERGAKAPAGANALL
                     LIEVDGEPDALDAAQAAIEHAASGEGLVELRSAGDDAEAATLWAARKALSPALRSISE
                     DKINEDVVVPVSRIPELVARLREIAAEFRIPIVNFGHAGNGNLHVNLLPRDPRERARA
                     EKALPLVFGKVIELDGTLSGEHGIGIAKRDFMPQAVGAGALSMMRGLKNLFDPEHILN
                     PGKLLPAEAGQGTGDPGPDHPDLRGRASAPEPQGPGTVVLGSSSP"
     gene            5809..6375
                     /locus_tag="VFK00_01510"
     CDS             5809..6375
                     /locus_tag="VFK00_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7591665.1"
                     /translation="MARIRACRACERQLPHGCRPVLQAHPEARLLIVSQAPGRKVHDT
                     GIPFNDKSGEKLRGWLGIDADTFYDPRRVAIVPMGFCYPGKGPSGDLPPRPECAPLWH
                     PQLLPRLTNIRLTLAIGGHAIRGMLGNRRKATLTETCAAWREYLDTEMLPLPHPSPRN
                     TAWFQRHPWFETDALPALQRHVQRVLHG"
     gene            complement(6424..7380)
                     /locus_tag="VFK00_01515"
     CDS             complement(6424..7380)
                     /locus_tag="VFK00_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7591666.1"
                     /translation="MSEDLDVPPARDPSVAAVLVSHHPDLALLRASLDALAAQAGWVV
                     VVDNASPGPGVRAICAGYRNVEFVPLPENRGLAAALNEGVAHARARPRVSHVLLMDQD
                     SVPETGMVDALKRVMDRLTETRKVAAVGPRFRDPREASDAPFVRIRFPVNRQLLCDGD
                     RAEVACDFLITSGSLIPLAVLDAVGGMEEGLFIDNVDLEWCFRATDRGYALFGVCDAR
                     MLHHHGAERQRVPGLPRGIVVHTPQRLFYMMRNRVLLYRRTYTPARWVAQDLPRMVVK
                     LLLFSLLVRPRRANLRWMLRGLRAGLRGQTGALRDADPDRAA"
     gene            complement(7497..8300)
                     /locus_tag="VFK00_01520"
     CDS             complement(7497..8300)
                     /locus_tag="VFK00_01520"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7591667.1"
                     /translation="MDMNIAPARIREIAAEFRRQKPYGYATVGGLFDRSLIAGVAREV
                     DSHLDRCAREPEIYASHRKYRLSRLEDMPEQTRDFIAYLNSPAFLSVLSEITGIADLR
                     PDPELRGGGIHAIGRGGFLKVHTDFNWHRGLSMHRRLNLLVYLNEHWQPDWRGDLELW
                     SEDAKRRVFALSPQLGNALLFETSDVSYHGHPDPLACPEGTFRTSIALYYYTATRPDA
                     GVVFGKTEMTNFVERPSEKFVTDKSRRLRHKLQLRVKKIAHSLKPRRHA"
     gene            complement(8494..>9172)
                     /locus_tag="VFK00_01525"
     CDS             complement(8494..>9172)
                     /locus_tag="VFK00_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7591668.1"
                     /translation="AAQANSICSCGPLDALAAWIAAGVAPGLERVVATSSMSAESKQA
                     SPVPAERAVSRRLRDGEASLAQACGQRGVEWTILRPTLIYGAGRDRSLTPLARRAMRW
                     RMFGLPAGSGLRQPVHAADVAVAVLRALDGAAAGRVVPVGGGERLTALAMFERVRASL
                     PVRTCPVRIPASAVRLGARLVPALRGPLTRLEQDLVADNGDLERLLAVHPRGFAPDRD
                     CWSHVRG"
BASE COUNT         1506 a         3212 c         3088 g         1366 t
ORIGIN      
        1 cgtgccggtc ggttgcccga ctcgcggtgg gctcttaccc caccgtttca cccttgccgg
       61 cctttcggct taggcggtct gctctctgtt gcactttccg tcggctcgcg ccgcccaggc
      121 gttacctggc accttgccct gtggagcccg gactttcctc ggcgtcccga aggacgacgc
      181 gactgtccgg ccgactccgc ggacgattat accgggaccg gggacgggga ccggaaaagc
      241 gtagcgccga tcaaccggct ggaacccgcc cattgcgatc cacctggagt gggtcttgat
      301 ccgccacgga gatacgaaag actgcgaggt tgaaacctgc ggcacgcggc tgttccccgc
      361 cgcatcgtcg gcatgcggcg gacgacgccg tttggacgga acggctacac gtctcccggc
      421 tcccgggccc cggtccgagg tccccgatct ccatacagcg ccttgcgcgg cgcaccactg
      481 atcgcagctg caagcttggc agcctgcgca ggcggcagtt cggctcgcag cagcgcgtaa
      541 acgcgctggc cttccgcgag cttcgcttcg gcgtcttcgc cgcgcccggc gacgaccaat
      601 acgaattcgc ccagccgctg ttcgcggtca tccgcgacgc gtgcggcgat ttcgccgagc
      661 gtcatcccga gatgggtctc gtgcaacttc gtgatctcgc gaagcagggc cgcctcgcgt
      721 tccgcgccga atgcctcggc gagatccgca cagcattcgg cgatccgatg cgaggactcg
      781 tagaacacca gcgtgcggac ttcgccggcc agttcgcgga gccgcgcgcg gcgcgcgggg
      841 ggcttcgacg gcaggaagcc cacgaaaacg aagcgatcgc tggggagtcc cgccaccgaa
      901 agcgctgcga tggcggcgca ggcaccggga acgggtgaca cccgcagacc gattccgcgc
      961 gccgcgcgca ccaggcggaa gcccggatca ctgatgagcg gcgtgccggc gtcggatacc
     1021 agcgccacat catcgccggc ctgcagcctc gcgagcaacg caggcacggc cgcgcgttca
     1081 ttgtgctcgt gcactgccgc cagcggcgtc gcaatcccgg cgtgcttgag caaggggcga
     1141 gtatggcgcg tgtcttccgc ggcgaccacg gcgacgcttc gcaagacctc gcgcgcacgg
     1201 tcggacagat cgccgaggtt gccgatcggc gtggcgacga cccacagggt tcccgccctg
     1261 ttcgcgttca tgatgcagtc ctgggattcc cgcacgatcc gttatcatcg ccgaaacgtg
     1321 ttcttttcag aagccggcgc gccgagggcg cagggaacca gatgcacagg atcgaccact
     1381 tcgcgtacgc cgtcctgctc gtcgcggcac ttttgctgac cgggggctgc gccacggtcg
     1441 gtccatccgc accggccgcc agtccgcagg cgcaggcgga gtacacgcat gcgcaggaac
     1501 tgtatcgatc cggcaatttc caggacgcgg cccgcgcctt cctcgcggcc gccaacagcg
     1561 atcccacgat ccgcgaccgc gcgacgctcg ccgcagccgc gagctaccgt tcgctcgggc
     1621 aaatcgggca gacggcggcc ctgctgcgac ggatcgatcc ccacggcctc tcgcccgccg
     1681 acgacgcccg ctatcgcgtg ctctccgcgg aggttgcgct gcaaggcggc caccccgata
     1741 ccgcactgaa gcaactggcg agcctgcccg ccgaccttcc cccggaaatc gagcagcatg
     1801 cgctcgacgt ccaggtccgc gcccatctcg ccaccggcga ccgcctcggc gcggcgcgcg
     1861 cgctggtgca gcgcaacgcc tcgctgccgc ccgcggcgca ggccggcaac caccagcagg
     1921 tcgtctcgat cctggcgggc atgggcagcg accggttgac gccgctgtat gcgtcgctcg
     1981 cggacagtga tccgttgaag ccgttcgtgg gacaggcgct tcggcaatcc ggcaaggcga
     2041 tgccacgcgc cctgccgcaa ccgaaccagc cggtcggaac attgacggcg acctcgatgc
     2101 cgccgcaggg ctactcgatg ccctcgacgg tggcgctgct gttgcccgcg acgggtcggg
     2161 tggcgacggc cggagcggcg gtgcgcgacg gatttttcac cgcctatttc gacaccccgg
     2221 tcacgcgtga acagcggccg gtggtgaagg tctacgacac gggtggaacg gccgacggcg
     2281 ccgcgaccgc atatcagcag gccgtcgccg acggcgccga actcgtggtc ggcccgctcg
     2341 cgcgcagcgc ggtcgccgcg gtattcgccc aaggcgcgct gccggtaccg gtactggcgt
     2401 tgaaccacgc ccaaggcgat gtccccacgc cggcccgcag catcgaattc tcgctgctgc
     2461 cggaagcgga tggcgcgcaa ctggccgcgc gcatgatcac cctgggactg cacgcgtcga
     2521 tcgtgttccg tggcatcgac gacaccgctt cgcgcacctt cgatgccttc aagacccagt
     2581 tcgaaagcct cgggggcgtg atcgcgaacg cgatcgtcct gcaaccgggc gacgtggact
     2641 ttgccgacca gatcgaggcc gcgctcgcgg gatcgggatc cgaaaccggc atcgtggtct
     2701 tgctgcggcc cgagcaggcg cgcctgctgc tgccgcaact gcggcttgca cgctcgacgc
     2761 tgccggtctt cgcgacatcg atggcgtacg acggcaccga ggatgcgacc gcgaacgtcg
     2821 acctcgacgg cgtgcagttc tgcgatgaac cctggctgtt cgatgcccaa gccggcctgc
     2881 ccgaccacgc catgctggct tcgctgctga cgaccgccgg tggcccggcc gcgcggctgt
     2941 tcggattcgg gatggatgcc tacgcgctgt tgccgtacct gggctggctg cgtacccatc
     3001 ccggcagtta cgtggcgggc gcgaccggcc agctgacgat ggataccttc ggccacgtgc
     3061 agcgcacgcc gatctgggtc cagttccagg acggcattgc gcggcccatc gccgccggac
     3121 tcgaagcgca acccgccgcg cccgacatcc cgacggaacc atgacaccgg gcggtgtcgg
     3181 ggcgcgtttc gagcaacgcg cgctggatgc cctggaacgc gcgggcctgg aactggtcga
     3241 acgcaattgg cgaacccgct tcggcgaact ggacctggtg atgcgcgatg gcgacacgct
     3301 cgttttcgtc gaagtccgct accggcaaca tgcgcgcttc ggcggcggag cggcgtcggt
     3361 cggcccggcc aagcgcggca agctcacgcg cgcggcgcag ggtttcctgc aggcgcaccc
     3421 gcggcttgcc gcgatgccct gccggttcga tgtggtagcg ttcgacggcc acgcggatgc
     3481 gcccagccac gcctggcacc gtgccgcgtt cgaaatctgc tgactggaag aaagaaatgc
     3541 gcaagacgat tctcctgacc accgccctcg cactcgtttg cctggtgtcc gcatgcagcg
     3601 tgttcacccc ccgccgcggc ttcaaccggg taatcaacga ccgcaacgtg cagatcaccg
     3661 cgcagcgcgc gctggacaac gatcgcgaac tggacggacg cgcgcatatc ggcaccaatg
     3721 tctacaacgg cgtgctgctc ctgatcgggg aagccggcac cgaagagatc aagcagcgtg
     3781 ccgaggccga cgtcagcggt tacgaaggcg tgcggcgcgt ggtcaacctc gtcgatgtga
     3841 tgccgctgca atcggtgggc cgttcggcgc tcgacgcctc gctgacggcg cgcgtcaaga
     3901 ccgcgctgct gcacgtcgac ctgccgggat tcgatcccgg ccgggtcaag atcagcaccg
     3961 cgcacgggaa cgtgtacctg atggggctgg tcagccacca ggaagccgac gcggtggtgg
     4021 atgtcgcgcg caacgtcgcg ggagtcgaca aggtcgtaca ggtcttcgaa tatacggact
     4081 gacgaaaagc agaacaggcc ggtgtggggg acgaagcggc tgtcgtgaaa ggtcaggcca
     4141 tggacggccg ttccggagtc tatggtgtgc atatgcaagg gttgcaaaca gtcgcaacga
     4201 agcagcgatc agcgatgatc gcgaagacat gagcctgccc gccgctttcg tcgatgagat
     4261 ccgcgagatt tttcccgccg atgcgctggc cctggacgag ggaacccgcg ccgcctacgg
     4321 ctacgacaat tcgcggcgcg aggcactgcc cgacgcggtt gtgttcccgg caagccatga
     4381 ccaggtcgcc gcgctggtcg tcgcctgccg gcggcaccgc atgccgctga ctgcccgggg
     4441 ccgcggcacc aacaccaccg gcgccacggt gccgatcgcg ggcggtatcg tcgcgagctt
     4501 cgagcgcatg aaccggatcg tgcggatcga gccgggcgac cgcctcgcgg tggtcgaacc
     4561 cggcgtgctg aacgccgacc tgcagactgc gctcgccgcg cacggcttgt tctggccgcc
     4621 cgaccccacc tctgcgccgt attgcaccat cggcggcaac ctcgcctgca acgcgggtgg
     4681 tccacacacc gtgaagtacg gcgccagccg cgacaacatc ctcgggctgc gcgcggtggc
     4741 cggcaacggc acctcattcc gcagcggcac gcacaccacc aagggcgcga ccggatacga
     4801 cctcacgcgc atcatcgtcg gctcggaagg cacgcttgcg ctgatcaccg aagcgacgct
     4861 gaagctgaca cccaagccca ccgcggtgcg cacgctgcgc gcggcctacg acggcatcca
     4921 ggcagccgcc gacgcggtcg cacggatcat ggcgcaaccg gtgacgccgc gcgcacttga
     4981 attcatggac gaggccgcat tgcaactcgt gcgcgagcgc ggcgccaagg cgccggccgg
     5041 agccaacgcg ctgctcttga tcgaggtcga cggcgagccc gatgccctgg acgccgcgca
     5101 ggcggcaatc gagcacgcgg cctccggcga aggactggtc gaactgcgca gcgccggcga
     5161 tgacgccgag gcggcgacgc tgtgggctgc gcgcaaggca ctctcgcccg cgctgcgcag
     5221 catcagcgaa gacaagatca acgaggacgt ggtggtgccg gtcagccgca ttcccgagct
     5281 ggtcgcgcgt ctccgggaga tcgccgccga gttccggatc ccgatcgtga atttcggcca
     5341 cgccggcaac ggcaacctgc acgtgaacct gctgccgcgc gacccgcggg aacgtgcccg
     5401 cgcggaaaag gcgttgccgt tggtgttcgg gaaggtgatc gaactcgacg gaacgctttc
     5461 cggcgaacac ggcatcggca tcgccaagcg cgatttcatg ccccaggcgg tgggcgcggg
     5521 cgcactgtcg atgatgcgcg gactgaagaa tctgttcgat ccggagcaca tcctgaaccc
     5581 ggggaagttg ctgccggcgg aagcgggaca ggggaccggg gacccgggac cggaccatcc
     5641 ggatctccgg ggccgagcgt cggcgccgga gccccaagga cccggtacgg tggtgctcgg
     5701 atcgagcagc ccatgagtgt ggtcactttg gggcgggttt cgcgagctgc agttctcccc
     5761 ggtccccggt ccccggtacc tggtcccgag ggcgacttcg acgccctgat ggcccgcatc
     5821 cgcgcgtgcc gggcgtgcga gcgccagttg ccacacggct gccggccagt gctgcaggcg
     5881 caccccgaag cacgcctcct gatcgtgagc caggcaccag ggcgcaaggt ccacgacacc
     5941 ggtatcccgt tcaacgacaa gagcggcgag aagctgcgcg gctggctcgg catcgacgcc
     6001 gacacgttct acgatccacg tcgggtcgcg atcgtgccga tgggtttctg ctatccgggc
     6061 aaggggccct cgggcgacct gccaccgcgc ccggaatgcg cgccgctgtg gcatccccaa
     6121 ttgctgccgc gactgaccaa catccgcttg acgctggcga tcggcggcca cgcgatccgc
     6181 gggatgctgg gcaaccggcg caaggcgacc ctgacggaaa cctgcgcggc gtggcgcgag
     6241 tacctcgaca cggagatgct gccgctgccg cacccgagcc cgcgcaacac ggcgtggttc
     6301 cagcggcacc cctggttcga aaccgacgcg ctcccggcgc tgcagcggca cgtgcagcgc
     6361 gtgctgcatg ggtaatcgat ccgccaatcc catgcgcgcc gacttcggca gacggtgtcc
     6421 ggcctacgcg gcgcgatccg gatcggcgtc gcgaagggct cccgtttgtc cgcgcaggcc
     6481 ggcgcgcagt ccgcgcagca tccagcgcag gttcgcgcgc cgcggccgca ccagcagcga
     6541 gaacaacaac aacttgacga ccatccgcgg caggtcctgg gcgacccagc gcgccggcgt
     6601 gtaggtgcgc cggtacaaca gcacgcggtt gcgcatcatg tagaacagtc gttgcggcgt
     6661 gtgcacgacg atgccgcgcg gcaggccggg cacgcgctgc cgctcggctc cgtggtggtg
     6721 gagcatccgc gcgtcgcaca ccccgaacag cgcatagccg cggtccgtcg cacgaaagca
     6781 ccattccaga tcgacgttgt cgatgaacaa tccttcctcc atcccgccga ccgcgtccag
     6841 caccgccagc gggatcagcg acccggaggt gatcaggaag tcgcaggcga cctcggcgcg
     6901 gtcgccgtcg cagagcagtt ggcggttgac gggaaagcgg atgcgcacga atggcgcgtc
     6961 cgaagcctcg cgcggatcgc ggaagcgggg accgacagcc gccaccttgc gggtttcggt
     7021 gagccgatcc atgacgcgct tcagcgcgtc caccatgccg gtttccggga cgctgtcctg
     7081 gtccatcagc agcacgtgcg aaacccgtgg ccgcgcccgg gcgtgcgcga cgccttcgtt
     7141 caacgccgcg gcgaggccgc ggttttccgg cagtggcacg aattcgacat tgcgataccc
     7201 ggcgcagatc gcgcgcacgc ccggcccggg actcgcgttg tccaccacca ccacccagcc
     7261 ggcctgcgcc gcgagcgcgt ccagcgatgc ccggagcagc gcgaggtcgg ggtgatggct
     7321 gaccagcacc gcggcaacgg aagggtcgcg cgctggcggg acgtccagat cctcagacac
     7381 accgcacccg accaaggccg cggcaacagg cggcgcctgc gcagaaggat cgggaacgag
     7441 gcaacgacgc ggcgcgccat ggtcgccacg tccacggccg catcaagatg ccgcgctcac
     7501 gcatggcgac gcggtttcaa cgagtgggcg atcttcttca cgcggagctg cagcttgtgc
     7561 cgaagccgcc gcgacttgtc cgtcacgaat ttttcggaag gccgctcgac gaaattcgtc
     7621 atctcggtct tgccgaacac gacgcccgca tccggccggg tcgcggtgta gtagtaaagc
     7681 gcgatcgatg tccggaacgt gccttcgggg catgcgagcg gatccggatg gccgtggtag
     7741 gagacgtcgc tggtctcgaa cagcagcgcg tttccgagtt ggggggacag cgcgaagacc
     7801 ctgcgcttcg cgtcctcgct ccagagttcg aggtcgcccc gccagtccgg ttgccagtgt
     7861 tcgttgaggt acacgagcag gttcaacctg cggtgcatcg agagtccgcg atgccaattg
     7921 aagtcggtat ggaccttgag gaaaccgccc cggccgatcg cgtgtatccc cccaccacgc
     7981 agctccgggt cggggcgcag gtccgcgatc ccggtgattt cggaaaggac ggacaggaat
     8041 gccggggagt tgaggtacgc gatgaagtcg cgggtttgtt ccggcatgtc ctcgagccgg
     8101 gacaaccggt acttgcgatg cgaagcataa atctcaggct cccgcgcgca gcggtcgagg
     8161 tgcgagtcga cctccctggc gacaccggcg atgagcgaac ggtcgaacag gcccccgacg
     8221 gtcgcgtagc cgtacggctt ctgccgccgg aattccgcag cgatttccct gatcctcgcg
     8281 ggtgcgatgt tcatgtccat gtcggcttct cccccgcgcg cattgtagtc gaggcgcggc
     8341 ggcggtcgga tgatgctcgc ttgcaaggct ttcgtgatgc tgaaaagaga tctgacgcgg
     8401 atgaacgcgg atgaccgtcg atcaaaaatc tttttatccg catcgatccg cgttgatccg
     8461 tgtcggaaat cgcttgtcgc caacggaccg ccgtcacccg cgcacatggc tccagcagtc
     8521 gcggtccggg gcgaagccac gcgggtgcac ggcgagcagg cgttcgagat cgccattgtc
     8581 ggcgaccagg tcctgctcca ggcgggtcaa cggcccgcgc agcgccggca ccaggcgcgc
     8641 gcccagccgc acggccgacg ccgggatgcg caccgggcaa gtgcgcaccg gcaggctcgc
     8701 gcgtacccgt tcgaacatcg ccagtgcggt gaggcgttcc ccgccaccga ccgggactac
     8761 ccgtcccgcg gcagccccat cgagtgcgcg caggaccgcg accgcgacat cggcggcatg
     8821 gaccggttgg cgcagcccgg atcccgcggg caatccgaac atccgccacc gcatcgcacg
     8881 tcgcgccagc ggcgtcaggc ttcggtcgcg cccggcaccg tagatcagcg tcggccgcag
     8941 gatcgtccac tcgacccccc gttgcccgca cgcctgcgcg agcgacgctt cgccgtcacg
     9001 caggcggcgc gacaccgcgc gctccgccgg cacgggcgat gcctgcttgc tttccgcgct
     9061 catcgagctg gttgccacga cccgctcgag accgggcgcc acgcccgccg caatccatgc
     9121 tgccaacgcg tccaaggggc cgcacgaaca gatcgaattg gcctgagcgg cc
//
LOCUS       DASSNH010000041         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_729492, whole genome shotgun sequence.
ACCESSION   DASSNH010000041 DASSNH010000000
VERSION     DASSNH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729492"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFK00_01530"
     CDS             <1..375
                     /locus_tag="VFK00_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7591669.1"
                     /translation="RQKVLDFGAQAFVDLENDALEDVGGVDLVFDVIGGDIQKRSAAL
                     VRPGGTLVTVVGPPEARPADGLAIDFVVESDRAQLTEIVQRVRDGRLRTNIGKVSDLD
                     DAVAAFNTTERTKGKTIIRVRP"
     gene            425..799
                     /locus_tag="VFK00_01535"
     CDS             425..799
                     /locus_tag="VFK00_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET7591670.1"
                     /translation="MTQLPHGLDDAFIARQRKRLLELRTQLIASGDAAGADEHTLQVA
                     AGEEPRDSVDDGDRLEREFDDEALLAHNLDRIATIDRALQKIRENTYGLSDGNGEPIG
                     RAHLEAVPEAIFTADELRARQG"
     gene            complement(986..1564)
                     /locus_tag="VFK00_01540"
     CDS             complement(986..1564)
                     /locus_tag="VFK00_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591671.1"
                     /translation="MVAASNAIVPGEHPVMGWGAVWAGWFLATGVAALLYAFGLAVGF
                     SGFNPGSPEIVAKGVSGGAIAWMILTWAASLWLGGMFATWFDGRNDTEMGVIRGLAVW
                     GVSVTATVLLIASGMTHLTFAAAAIPTTEGIPTVVDPAALAQYTGRIMWTAFGCALAS
                     LVTAAFGGWIGAHHVHRVYHLRTYTPHPAVRR"
     gene            complement(1586..1726)
                     /locus_tag="VFK00_01545"
     CDS             complement(1586..1726)
                     /locus_tag="VFK00_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591672.1"
                     /translation="MKRLKLAFRRNGDPNRQKGAMGYVLAWLLGIPIPILIIVALLRS
                     CA"
     gene            2191..>2301
                     /locus_tag="VFK00_01550"
     CDS             2191..>2301
                     /locus_tag="VFK00_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7591673.1"
                     /translation="MKIATYNVNGIRARLPLLLRWLEAESPDIVCLQELKA"
BASE COUNT          446 a          789 c          717 g          349 t
ORIGIN      
        1 cgccagaagg tactcgactt cggcgcgcag gcgttcgtcg acctcgaaaa cgacgcactg
       61 gaagacgtcg gcggcgtcga cctggtattc gatgtcatcg gcggcgacat ccagaagcgc
      121 tccgcggccc tggttcgacc cggaggaacg ctcgtgaccg tcgtcggacc gcccgaggcg
      181 cggcccgccg acggcctcgc gatcgacttc gtcgtggaat ccgatcgcgc acaattgacc
      241 gaaatcgtcc agcgggtgcg ggacggacgc ctgcgcacga acatcggcaa ggtgtcggac
      301 ctcgacgacg ccgtcgctgc cttcaacacg accgagcgaa ccaaggggaa aacgatcatc
      361 cgcgtccgtc cgtgaggact cggcacgcgg ccgatccact caacctgcgc agcaaggagc
      421 agccatgaca cagttacccc acggtctcga cgatgcattc atcgcccgcc agcgcaaacg
      481 tttgctcgaa ctgcgaaccc aattgattgc cagcggcgat gccgccggcg cggatgagca
      541 tacgctgcag gttgcggcag gcgaggaacc ccgggattcc gtcgacgacg gtgaccgcct
      601 ggaacgagaa ttcgacgacg aagcgctact cgcccacaat ctggacagga tcgcgaccat
      661 cgatcgcgcc ttgcaaaaaa tccgggagaa cacctacggc ctttccgacg gcaacggcga
      721 acccattggc cgcgcccatc tcgaggccgt gcccgaggcg attttcacgg cagacgaact
      781 ccgcgcccgc caggggtgaa acggggcggc cggggtgctg gacatcgctg tcccagccac
      841 cgagtttcgt ccgtcaggcg ggtacgggtg cgctggaccg ctcgcctggc ggccggcttt
      901 cttcaccgcc agcccgcgca gccgtaggcc ggcgtgtccg cggggctttc cacgccgcta
      961 cacgcgagcc ccgcgtaggg ccgcgctacc ggcgaacggc ggggtgcggc gtgtaggtgc
     1021 gcaggtgata cacgcggtgc acatggtgcg cgccgatcca gccgccgaaa gccgccgtca
     1081 ccagcgaagc cagcgcgcaa ccaaacgccg tccacatgat tcgcccggtg tattgcgcca
     1141 aggcagcggg atcgacgacg gtgggaatac cctcggtggt gggtatggcc gcggcagcga
     1201 aagtcaggtg cgtcatgccg ctggcgatca gcaggaccgt ggccgttaca gaaacgcccc
     1261 acacggccag gccacggatc acgcccattt cggtgtcgtt gcggccgtcg aaccatgtcg
     1321 cgaacatgcc gccgagccac aaggacgcgg cccaggtgag gatcatccag gcgatggctc
     1381 cgccggacac gccttttgcg acgatctcgg gacttccagg attgaagccc gaaaaaccca
     1441 cggccagtcc gaacgcgtag agcaacgctg ccacaccggt ggccaggaac caaccggccc
     1501 agacagcgcc ccaccccatt accgggtgtt cacccgggac gattgcattg cttgcggcga
     1561 ccatgtcggc ttctccttgt tgtactcacg cacagctacg cagcagcgcc acgatgatca
     1621 ggatcgggat gggaatgccg agcaaccacg cgaggacgta acccatcgcg cccttctgcc
     1681 tgtttggatc cccgtttctg cggaaggcca gcttcagtcg cttcataacc cgcctcgcac
     1741 gttgatgagg tggctcacta gaccacgcgg ttcgtgactt ccgtgtgaag cacgacccat
     1801 ggcgccgagc gggggaaatg cctgattcaa aaaggataaa ccgtgccccg gaaccactga
     1861 aatccggcgc ggcagcagtc atcgcgccgt cctggtcgat gacgcggacc aaccctcatc
     1921 catggtcgtg cggcatccgg gtcgaaccct cgcggagcga ggccgatacg ctcgccgccc
     1981 tttcattcat caattcgcat cagcgcgacg ccagcgccct catcccggac cgctcgtgga
     2041 tgcgcaagcc cgtcgcaccc gcgatccggc cggccgggta atactttgtc atccggggcc
     2101 acccgcgctg gggctgcgac gccaagcgga gcgcctggga cgggaacgct acttccaacc
     2161 ggatcggatc agcgaggaca ctccacagcc atgaagatcg ccacctacaa cgtcaatggc
     2221 attcgcgcga ggctgcccct gctgcttcgc tggctcgagg cagagtcgcc ggacatcgtc
     2281 tgcctccagg agctcaaggc g
//
LOCUS       DASSNH010000042         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1217466, whole genome shotgun sequence.
ACCESSION   DASSNH010000042 DASSNH010000000
VERSION     DASSNH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217466"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFK00_01555"
     CDS             <1..373
                     /locus_tag="VFK00_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7591674.1"
                     /translation="LLLAAALGLLFVTALGITGLASFWVQQRTRTIGIRRAIGATRGD
                     ILRYFQTENFLIVTFGVVVGVALAIGLNLLLMKHYELPRLPLWYLPVGAVVLWVLGQL
                     AVLAPALRASRVPPVVATRSV"
     gene            589..1929
                     /locus_tag="VFK00_01560"
     CDS             589..1929
                     /locus_tag="VFK00_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7591675.1"
                     /translation="MRTVLIIDDQAAVRDALSLLLSLNDIRPLAAGMPQEGLGLLARE
                     RVDLVIADMNFSADTTSGEEGVALFHAIRERHADLPVILLTGWSHLETAVSLVKAGAA
                     DYMSKPWDDAKLLATVENLLELSENARTSERARMTRRRRREALERDFDLCGIVFASDE
                     MLRVLELACQVARADVPVLITGPNGAGKERIAEIVHANSAVRGKPLVTVNCGALPSEL
                     IEAELFGAEAGAFTGATRAREGRFEVADGGTLFLDEIGNLPAAGQMKLLRVLESGEFE
                     RLGSTRTRKVKVRVVSATNADLPAMIRDGRFREDLYYRMNTIEIALPPLAERPDDILP
                     LAEFFLDGAAVLSDEAREVLVAYPWPGNVRELRNTIDRARLLCGRDAISVAHLGLPAA
                     APIVSRSLDDPPREAVEQALQRAGGVISQAARTLGLSRQALYRRMQRYGIPTEG"
     gene            2134..3447
                     /locus_tag="VFK00_01565"
     CDS             2134..3447
                     /locus_tag="VFK00_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7591676.1"
                     /translation="MRISSLEGKLALMLVAAAAAAAFLSAALATWTGSLWLAAILAVL
                     ACLVPLLWGAHAAMQPVRRLLRALSGSVASYRDGDFSISLRTRRRDELGQLIEAHNEL
                     GHALRAQRQDLVQRELLLDTVVQHSPTALVLTDARDHIIYANVSARHLFNEGKNPVGM
                     EFEELLDACPEPMRQALAASEDSLFGVEMGNEEEYFHLSRRDFRLQGRAHRLYLVQRM
                     TRELSRQEVAVWKRVIRVISHELNNSLAPISSLAHSGAVLARRSDLAQLPRVFEGIGE
                     RVRHLHSFTAGYASFAKLPQPNPAPIAWDAFLDALRMHCEFRLLGEPPPDPGQFDAAQ
                     IEQVLINLIKNAHESGSGADEVTLAIQHVMHHWRIEVADRGPGMSDTVLAQALLPFYS
                     TKRSGTGLGLALAREIVEAHGGRIVLANREGGGLRVAVSLPDHRS"
     gene            3640..>3974
                     /locus_tag="VFK00_01570"
     CDS             3640..>3974
                     /locus_tag="VFK00_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET7591677.1"
                     /translation="MMEARSAGGGESGGTLVFDEQRRARIGFVVELARRLHQYGAAAP
                     RLEQAIGNVTQQLGLACDVLSTPTSIVLSFGAPEGDGIAEITQVVRIAPGEVNLARLC
                     RADEIADQVA"
BASE COUNT          613 a         1297 c         1412 g          652 t
ORIGIN      
        1 cctgctgctg gccgcggcgc tgggcctgtt gttcgtcacg gcgctcggca tcaccggact
       61 ggcgagtttc tgggtgcagc agcgcacccg caccatcggc atccgccgcg caatcggcgc
      121 gacccgcggc gacatcctgc gctatttcca gaccgagaat ttcctgatcg tcacgttcgg
      181 cgtggtggta ggcgtcgcgc tggcgatcgg cctgaacctg ttgttgatga agcactacga
      241 attgccgcgc ctgccgctgt ggtacctgcc ggtcggcgcg gtggtgctgt gggtgctcgg
      301 ccagctcgcg gtgctggccc ccgcgttgcg ggcctcgcgc gttccaccgg tggtggcgac
      361 gcgcagtgtg tgatgtatac ccccttcgga acgaagggcg gagcgaagcg aacgcgcgcg
      421 tagcgcggat gcgaagcatc ggggggatgt cgtggcggaa gcaagagcca tccccctcgg
      481 tcccccttcc ttcggaaagg gggaagacaa gcgcgccctt ttgcacgcga aggggcagga
      541 caagaaaagc atgatagcct cgcgcgttgt ccaatctgct ggagagccat gcgcacggta
      601 ttgatcatcg acgaccaggc cgcggtgcgc gacgcgctgt cgctgctgct gtcgctcaac
      661 gacatccggc cgctcgccgc gggaatgccg caggagggac tggggttgct ggcgcgcgag
      721 cgggtggacc tcgtgatcgc cgacatgaac ttcagcgccg acaccacctc gggcgaggag
      781 ggggtcgcgc tgttccatgc gatccgggag cggcatgccg acctgccggt gatcctcctg
      841 accggctggt cgcaccttga gaccgcggtg agcctggtga aggcgggcgc cgccgattac
      901 atgtccaagc cctgggacga cgccaagttg ctggcgacgg tcgagaacct gctcgagctt
      961 tccgaaaacg cccgcaccag cgagcgggcg cgcatgaccc gccgccgccg tcgcgaggcg
     1021 ctcgagcgcg acttcgacct gtgcggcatc gtgttcgcct cggacgaaat gctgcgggtg
     1081 ctggaactgg cgtgccaggt cgcccgcgcg gacgtgccag tcttgatcac cgggcccaac
     1141 ggcgccggca aggaacgcat cgcggagatc gtgcacgcca attcggcggt tcgcggcaag
     1201 ccgctggtga ccgtgaattg cggcgcgctc ccgagcgagc tgatcgaggc cgagctgttc
     1261 ggtgccgagg ccggcgcttt caccggcgcg acccgcgcac gcgagggccg cttcgaggtg
     1321 gccgacggcg ggacgctgtt cctggacgag atcggcaact tgcccgctgc aggtcagatg
     1381 aagctgctgc gcgtgctgga atcgggcgag ttcgagcgcc tcgggtccac ccgcacgcgc
     1441 aaggtgaagg tgcgggtcgt cagcgcgacc aacgccgacc ttccggccat gatccgcgac
     1501 ggcaggttcc gcgaggacct ctactaccgg atgaacacca tcgagatcgc cttgccgccg
     1561 ctcgccgagc ggcccgacga catcctgccc ctggccgagt ttttcctcga cggcgcggcg
     1621 gttctgtcgg acgaagcccg cgaagtcctg gtcgcgtacc cgtggccggg caacgtgcgc
     1681 gaactccgga acaccatcga ccgcgcgagg ttgctgtgcg gccgcgatgc gatcagcgtt
     1741 gcgcacctcg gcctcccggc ggcggcaccc atcgtgtcgc gaagcctcga cgatcctccg
     1801 cgggaggccg tcgagcaggc gctgcagcga gcgggcggcg tgatcagcca ggcggcgcga
     1861 acgctcgggc tatcacggca ggcgctatac cggcgcatgc agcgctacgg gattccgacc
     1921 gaagggtgag aagccgcgtt tgcgtgatgc cggggcacca gatgtaggtt gggttgactc
     1981 cggattcatc cggaggaacc ccaacaacgc cagacgatcg catcgccatc gttgggcttc
     2041 tcccggatgg ggcccgggat cagcccaacc tgcgcttttc gagtgttgtt aggctttcgc
     2101 acttccgcac cccgagcctc gaaccggaaa tccatgcgca tctcgtccct cgaaggcaaa
     2161 ctcgcgttga tgctggtcgc ggccgcagcg gcggcggcgt tcttgtcggc cgcgctggcg
     2221 acctggacgg gaagcctgtg gctggccgcg atcctcgcgg tgctcgcgtg cctcgtgccg
     2281 ctcctttggg gggcgcatgc cgcgatgcag ccggtgcggc gcctgttgcg tgcgttgtcg
     2341 ggaagcgtcg cgagctatcg cgacggtgac ttcagcattt cgctgcgtac ccgccgccgc
     2401 gacgagctcg gccagttgat cgaggcccac aacgaactgg ggcatgcgtt gcgcgcgcag
     2461 cgccaggacc tggtgcagcg ggaactgttg ctggacacgg tggtgcagca ctcgccgacg
     2521 gcgctggtgc tgaccgacgc ccgcgaccac atcatctatg cgaacgtgtc tgcgcggcat
     2581 ctgttcaacg aaggcaagaa tccggtcggc atggaattcg aggaattgct cgatgcctgc
     2641 ccggagccga tgcgacaggc gctggctgcc agcgaggaca gcctgttcgg tgtcgagatg
     2701 ggcaacgaag aggaatactt ccacctgtcc cggcgcgact tccggctgca ggggcgggcg
     2761 caccgcctgt acctggtcca gcggatgacc cgcgaactgt cgcgccagga agtcgcggtg
     2821 tggaagcgcg tgatccgggt gatcagccac gagctcaaca actcgcttgc gccgatttcc
     2881 tcgctggcac attcgggcgc ggtgctggcg cgccgcagcg atctcgccca gctcccgcgg
     2941 gtgttcgaag gcatcggcga acgcgtgcgg cacctgcatt cgttcaccgc gggctatgcg
     3001 agtttcgcca agttgccgca accgaacccg gcaccgatcg cgtgggacgc gttcctcgac
     3061 gcgctgcgga tgcactgcga gttccgactg cttggcgagc caccgccgga tcccgggcaa
     3121 ttcgatgcgg cgcagatcga gcaggtcctg atcaacctga tcaagaacgc ccacgaatcg
     3181 ggcagcgggg ccgacgaggt gaccctcgcg atccagcacg tgatgcacca ctggcggatc
     3241 gaggttgccg atcgcggccc gggcatgagc gataccgtgc tcgcgcaggc gctgctgccg
     3301 ttctattcga cgaagcgctc gggcaccggg ctcggattgg cgctggcgcg cgagatcgtc
     3361 gaggcgcacg gtggccggat cgtgcttgcc aaccgcgagg gcggcggcct gcgggtggcg
     3421 gtgtcgctgc ccgatcaccg aagctgattt cgacgggcgg gcgccgggcg cggctggcta
     3481 tactgcgccg ggattttccg gatcgcacgg cgagcgtgcg gcgcgccggt ttccatggca
     3541 ggcggggcgc acgccggcgc aacacttgac ggagcggatc gctgcagtgc atggcggcca
     3601 ttgcgtacgc cggctgcgcc cgcgggcggg gatgcaacga tgatggaagc acgatcggcc
     3661 ggtggcgggg aatcgggcgg cacgctggtt ttcgacgaac agcgccgcgc gcggatcggt
     3721 ttcgttgtcg aacttgcgcg ccggctgcac cagtacggcg cggcggcgcc acgcctggaa
     3781 caggccatcg gcaacgtcac gcagcaactg ggcctcgcct gcgacgtgct gtccacgccg
     3841 acttcgatcg tgctgtcgtt cggcgcaccc gagggcgacg ggatcgccga aatcacccag
     3901 gtggtgcgga tcgcgccggg cgaggtgaat ctcgcccgct tgtgccgtgc cgacgagatc
     3961 gccgaccagg ttgc
//
LOCUS       DASSNH010000043         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_680124, whole genome shotgun sequence.
ACCESSION   DASSNH010000043 DASSNH010000000
VERSION     DASSNH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680124"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFK00_01575"
     CDS             complement(<1..417)
                     /locus_tag="VFK00_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET7591678.1"
                     /translation="MSGVRFDATALGVFAFFFVLVTVLGFFAARWRRGDLSQLHEWGL
                     GGRRFGAFVTWFLLGGDLYTAYTLIAVPALVYAVGAYGFFAMPYTVFVYPIVFLTMPR
                     LWNACRKHGWITSADFVEGRYGSRGLALAVAVTGVLA"
     gene            complement(414..596)
                     /locus_tag="VFK00_01580"
     CDS             complement(414..596)
                     /locus_tag="VFK00_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HET7591679.1"
                     /translation="MPRKLTPWLLLIPFIALLCVPLYNRMQPTIAGFPFFYVWQFAWV
                     VLAALLTWIVHRGWRA"
     gene            681..896
                     /locus_tag="VFK00_01585"
     CDS             681..896
                     /locus_tag="VFK00_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HET7591680.1"
                     /translation="MEQKRVQFDFEVAFSNGGGLKGWDFRLDIDGDDISDEALADYVV
                     RDLRLLMVGSVAVSNKKIISEPHKRDS"
     gene            950..1327
                     /locus_tag="VFK00_01590"
     CDS             950..1327
                     /locus_tag="VFK00_01590"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7591681.1"
                     /translation="MTSNVNRGPRQIWRALIWSIKGLRQGWQLEASFRLEVVLFVILF
                     PIGLWLGNGAIEKALLAGSLLPVLAAELLNSGLEALVDKLWPERHELAGRAKDMGSAA
                     VFLLMLNVLVVWIVILGARTGAW"
     gene            1327..1818
                     /locus_tag="VFK00_01595"
     CDS             1327..1818
                     /locus_tag="VFK00_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET7591682.1"
                     /translation="MDPLRWLRHWFHAPASRAFPPQTMERIQEAIAAGEVRHDGEICF
                     AVEARLPARYLIGGRRVRARAEGVFADLKVWDTERRTGVLIYVLLGDYTIEIVPDRGV
                     AARVPGESWARIVAQMGEQFRREQWEDGALLGIAAIHALLETYLPPGPGRRDALPDQP
                     IIL"
BASE COUNT          368 a          638 c          725 g          318 t
ORIGIN      
        1 cgcgagcacg ccggtaaccg cgaccgccag cgccagtccg cggctgccat aacggccttc
       61 gacgaaatcg gccgaggtga tccagccgtg ctttcggcat gcgttccaca ggcgcggcat
      121 ggtcaggaac acgatcggat atacgaagac ggtatacggc atcgcgaaga agccataggc
      181 gccgaccgcg tacaccagcg cgggcaccgc gatcaacgtg taggcggtat aaaggtcgcc
      241 gcccagcagg aaccaggtga cgaatgcgcc gaagcggcgt ccgccgaggc cccactcgtg
      301 caactgcgag agatcgccac gccgccagcg ggctgcgaag aaaccgagaa ccgtgaccag
      361 cacgaagaag aacgcgaaca caccgagcgc ggtggcgtcg aagcggacgc cgctcatgcg
      421 cgccacccgc gatgcacgat ccacgtcagc agcgcggcga gcacgaccca cgcgaattgc
      481 cagacataga aaaacgggaa tcccgcgatc gtcggctgca tgcggttgta aagcggcacg
      541 cacaacagcg cgatgaacgg aatcagcaac aaccacggcg tgagtttgcg cggcatgggc
      601 tgcgtgctcc tgggtagcgg ggcaggacgt ccggcggcgg ccgtggcatc atacgcggct
      661 tcgcagccga tcacgattcc gtggagcaaa agcgcgtcca gttcgacttc gaggtcgcct
      721 tcagcaacgg cggcggcctc aagggttggg atttccggct ggacatcgac ggcgatgaca
      781 tttccgacga agcgttggcc gactacgtcg tgcgcgacct gcggctgttg atggtcggta
      841 gcgtggcggt cagcaacaag aagatcatca gcgagccgca caagcgcgac tcgtgaaggg
      901 gccgcgcgat gcacgaattc gctgaccgcg gccggcgccg ggaggttcga tgaccagcaa
      961 cgtcaaccgt ggaccgcgcc agatctggcg ggcgctgatc tggtcgatca agggccttcg
     1021 ccagggctgg cagctcgagg cttcgtttcg cctcgaagtg gtcctgttcg tgatcctttt
     1081 tccgatcggg ctgtggctgg gcaacggcgc gatcgaaaag gcactcctgg ccggttcgct
     1141 gttgccggtg ctggcggcgg aattgctgaa ctcggggctg gaggcgctgg tcgacaagtt
     1201 gtggcccgag cgccacgaat tggcgggacg cgccaaggac atgggttccg cggcggtgtt
     1261 cctgctgatg ctgaacgtgc tggtggtctg gatcgtgatc ctcggggccc gaacgggggc
     1321 gtggtgatgg acccgctgcg ttggttgcgc cactggttcc acgcgcccgc gagccgcgcc
     1381 ttcccgccac agacgatgga acgcatccag gaggcgatcg ccgccggcga ggtgcggcac
     1441 gacggcgaga tctgtttcgc cgtcgaggcg cggctgccgg cgcgttacct gatcggcggc
     1501 cggcgggtgc gggcgcgtgc cgaaggcgtg ttcgcggacc tcaaggtgtg ggacaccgag
     1561 cgccgcaccg gcgtgctgat ctacgtgctg ctcggcgatt acacgatcga gatcgttccc
     1621 gatcgcggcg tggcggcgcg cgtgcccggg gaatcgtggg ccaggatcgt cgcgcagatg
     1681 ggcgagcagt tccgccgcga gcagtgggaa gacggcgcgc tgctcggaat cgccgcgata
     1741 cacgcgctgc tcgagaccta cctgccgccg ggaccgggcc gtcgcgacgc gctgccggac
     1801 cagccgatca tcctgtaggg tgggcttcat ccgtcatcat tctttctcta cgtcgtcatt
     1861 ccgcggccac gccgtttgcc ggcgcgggaa ccgactgcga ctgcaaacgc gaacgccaca
     1921 ggcgacccga agcgcgcggc agggatggcg cgagtaaatc tgctgtcttc ggaatccatg
     1981 taggttgggc tgatcccgga ctcgtccggg agaagcccaa cggccgccga cccggtggcg
     2041 tgtgagggg
//
LOCUS       DASSNH010000044        15071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_318863, whole genome shotgun sequence.
ACCESSION   DASSNH010000044 DASSNH010000000
VERSION     DASSNH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318863"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..851
                     /locus_tag="VFK00_01600"
     CDS             372..851
                     /locus_tag="VFK00_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7591683.1"
                     /translation="MRPLSWLSIPIVAATLACAACAAAPPAPVAPAGTPTAVLNGHRF
                     SVEIADTPAQREHGLMDRTSMPGDHGMLFVFPDAEPRTFWMKNTLIPLDILYFDNARR
                     LVSVQEDVPPCRADPCPVYPSIAPARYVLELNAGVAAKLGVRRGDAITFSGLPSGTQ"
     gene            complement(1142..1339)
                     /locus_tag="VFK00_01605"
     CDS             complement(1142..1339)
                     /locus_tag="VFK00_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7591684.1"
                     /translation="MNAPAATVVAYRRFMCVVCGFIYDEEAGLPEEGIEPGTRWEDIP
                     DTWTCPDCGVTKDDFEMIEVD"
     gene            1435..2097
                     /gene="thiE"
                     /locus_tag="VFK00_01610"
     CDS             1435..2097
                     /gene="thiE"
                     /locus_tag="VFK00_01610"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7591685.1"
                     /translation="MPQESHFRSRGPSTRPGTGLYAITDGPRDDLLQVARAALEGGAA
                     MLQYRDKTRDGGRRLREARALADLCTSFGVPLIVNDDVELARSSGGSGVHLGKGDGTI
                     ADARGALGTGAIVGMSCYDSLERARDAAAEGADYLAFGAFFPSITKPGARHATPDLLR
                     KAREFDRPLVAIGGITAENGRPLISAGADYLAVVSGVFGANDVRAAARRYAELFDADQ
                     SA"
     gene            2201..3487
                     /gene="hemL"
                     /locus_tag="VFK00_01615"
     CDS             2201..3487
                     /gene="hemL"
                     /locus_tag="VFK00_01615"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7591686.1"
                     /translation="MASNHELFQRARQVLPGGVDSPVRAFKSVGGEPFIAARADGPYL
                     WDVEGKRYIDYVGSWGPMIAGHNHPRVREAVERAVRDGLSFGTPCAAEAELAEAIREL
                     IPSLEMLRFVNSGTEATMSALRLARGATGRARFVKFEGCYHGHGDSFLVKAGSGALTF
                     GVPDSPGVPKALADLTITLPYNDAGAARALFEEAGKHIACVIVEPVAGNMNCIPPQSG
                     FLETLRELCTRDGALLIFDEVMTGFRVAAGGAQALYGVTPDLTTLGKVIGGGMPVGAY
                     GGRRDLMEQVSPAGPVYQAGTLSGNPVAMAAGLAMLDLVRGDGFHAELERKTRVLTEG
                     LAACAHEAGVPFSVNRVGGMFGLFFSAGPVETFLQATSCDIAAFRRFFHVMLEQGVYF
                     APSPFEAGFMSTAHGDNDIAHTLEAARVAFAEAERA"
     gene            complement(3576..3998)
                     /locus_tag="VFK00_01620"
     CDS             complement(3576..3998)
                     /locus_tag="VFK00_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7591687.1"
                     /translation="MKYLHSMVRVRDLDASLRFYCEGLGLREARRRDVPEGRFTLVFL
                     SGPDSPDAEIELTWNWDSQEDYGSARNFGHLAFRVDDIYATCAHLQSMGVAINRPPRD
                     GHMAFVRSPDLISIELLQEGDPLPPSEPWASMPNIGTW"
     gene            4075..5202
                     /locus_tag="VFK00_01625"
     CDS             4075..5202
                     /locus_tag="VFK00_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7591688.1"
                     /translation="MDPKFDTLAVHAGAEPDAATGAVAPPIQLSTTFVHDPARGTDAY
                     SYQRQDNPTQHRLETALAALEGGERALAFASGMAAVAAVLDTLQRGERVVVPEDCYVG
                     TRGYVGEVLPERGVEVVTIDTTDLEAVAQACGDGASLLWAESPSNPRMRISDLAELAR
                     IAHAAGARFACDNTFATPVLQRPLALGADIVMHSTTKYFGGHSDVLGGALVFARNGDF
                     AARIAQRRVLTGSILAPFSAWLTLRGCRSLPARMARHCENARKVAEFLAGHPAIERVN
                     HPGLPEHPGHAIASRQMRDFGAMLSIELRGGYDAAVAVARNTRIFTNATSLGGCESLI
                     EHRASAEGEHTQAPPALVRISVGLEDAGDLITDLDQALSFS"
     gene            complement(5197..5901)
                     /locus_tag="VFK00_01630"
     CDS             complement(5197..5901)
                     /locus_tag="VFK00_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET7591689.1"
                     /translation="MRRADALRQLGTTQQKLLRRLLLAPQGANVEELCKALGVTHNAV
                     RQHLSALMAQGFVARGESSPSGGRPRACFVLLPAGRELFPRNYALIAGGVLEYLYSHA
                     GAQAVQAMLSSMGAKLGEDAAARVATAGSNDDAARLLAEQLDTLGYEAQVADADGHME
                     VEAWNCVFHSLARTHPDICKFDLAFMGAATGRPVQRTQCMLHGAPACRFRLGAPPGKT
                     GGTNHDAGAAGDTGSS"
     gene            complement(5906..6550)
                     /locus_tag="VFK00_01635"
     CDS             complement(5906..6550)
                     /locus_tag="VFK00_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591690.1"
                     /translation="MKPLTIACALALGLGAALAAPPVFAHPAATAASTPAAPQLQATM
                     RSLLHGHIVATRDYALAVHAGNQAKAQQAADDAVANAKQIAGAVASFYGKPAGDQFLK
                     LLAGHWGGVKNLTDSVQAGNKAGEQQAMQDLASNGSDIAKFLAGANPNLPEQTVNGLW
                     MMHVSDHKTQIEQMMANAPEAEQSQTWEHMQKHADTIADALAGAIAKQFPDKAH"
     gene            complement(6810..7400)
                     /locus_tag="VFK00_01640"
     CDS             complement(6810..7400)
                     /locus_tag="VFK00_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7591691.1"
                     /translation="MKRHEIPLFPLNAVLFPGSTLGLRIFELRYIDMVRECARSGGTF
                     GVCLIMEGQEAGEPALPAAIGTLARIIDFDTLPDGLLGISVTGGARFRVQKTRIRSNG
                     LVIGEVRRWPDEPRVPVPVEFSLLPTILERLAEQAGLSWRNGPREQYDNASWVGFRLA
                     ELLPLGDAERQHLLEITDPLERLGELRDAMPRFQKA"
     gene            complement(7410..8861)
                     /gene="mpl"
                     /locus_tag="VFK00_01645"
     CDS             complement(7410..8861)
                     /gene="mpl"
                     /locus_tag="VFK00_01645"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET7591692.1"
                     /translation="MRIHILGICGTFMGGVAALARELGVEVEGSDANVYPPMSDQLRA
                     LGITLREGWSAEYLKPAPDLVVVGNALSRGNPAVEYMLDARLPYVSGPQWLGEQVLRD
                     RQVLAVAGTHGKTTTTSLLAHLLESAGAAPGFLVGGVPGNFGVSARLRGPVPSSGAAR
                     KRKGGVSTPLLPKGEGNNASPAATPFVLEADEYDTAFFDKRSKFVHYHPDVAILNNLE
                     YDHADIFPDVAAIQRQFHHLVRTVPGNGRLVVNAEDARLAEVLAMGCWTPVETFGIDA
                     GDWRAQLVEPDGSAFAVTRRGQPVGEVRWSLLGRHNVMNALAALAAATAAGIDPAALL
                     PAFATFASVKRRMESLGDVNGVQVYDDFAHHPTAIATTLAGLRARVGNARIVAALEPR
                     SNSMRQGAHVAALAPSLADADRVLLLQRPELSWDAGRVTASLEGRGRAVRSVEELVQA
                     LVSEIRPGDHVVFMSNGGFENAPRRFLEALRGG"
     gene            complement(8879..9445)
                     /locus_tag="VFK00_01650"
     CDS             complement(8879..9445)
                     /locus_tag="VFK00_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591693.1"
                     /translation="MNLLGVLASVRDLCLFRGGPEEMPYSPRLLVGLLVACGVVQVVF
                     NLRDGVQPALAAGAVIGSLAIIVTVFLLLRRRGKSERFVQTTTALAVVYLLYDVVSNL
                     LTLLLPMDELRKQLTSQPPHLPALSGPQTLAMVVIAALGIWQLCLWVGILRRSLEIPV
                     AGGVLVFLLLVFVNLVATAVAASVIGLA"
     gene            complement(9442..10017)
                     /locus_tag="VFK00_01655"
     CDS             complement(9442..10017)
                     /locus_tag="VFK00_01655"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7591694.1"
                     /translation="MRIVLLGAPGSGKGTQAKRLKVELGVAHISTGDLLRAAVKAGTP
                     LGLKAKAVMESGQLVSDDIVLGLLEERLAQPDTEPGFILDGYPRNLAQAGALDALLAR
                     LGQPLDAVVKLNVPDADIIKRCEIRFEQEHRADDNPETVRKRLAVYAAQTAPVADFYS
                     ERGLLAQIDGVGDIEVIGERLLAALRARANA"
     gene            10246..11517
                     /locus_tag="VFK00_01660"
     CDS             10246..11517
                     /locus_tag="VFK00_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7591695.1"
                     /translation="MTATPKPTGRLLYAQSGGVTAVINATAAAVIEACRKHQVAVYAA
                     RNGILGALREDLIDTSKESPSAIAALKHTPGGAFGSCRVKLKSLEDDRAKYERLIDVF
                     RAHDIRWFLYNGGNDSADTALKLSKLGKAAGYDIRCIGVPKTVDNDLAVTDCCPGFGS
                     VAKYTAVSLRECALDVMSMMDTSTKVFVMEVMGRHAGWIAAAAGLAGARPDDAPHIIL
                     FPELEFDEEKFLARVRETVDRAGCCVIAASEGIKYADGKFVADAHAGTDAFGHTQLGG
                     VAPALAGMVRGKLKLKTHWAMPDYLQRSARHIASKTDLDQALAVGTAAVELALAGENA
                     VMPVIRRTSDAPYRWKIDTAKLDQIANREKKLPRSFIAKDGFHITAAARKYLSPLIRG
                     EAPPPYGKDGVPGYVTLKNVTVKKKLGSFEL"
     gene            complement(11691..12515)
                     /locus_tag="VFK00_01665"
     CDS             complement(11691..12515)
                     /locus_tag="VFK00_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET7591696.1"
                     /translation="MNAHRFAARRAAFRKLHETGCFLIPNPWDAGSAHALERLGFRAL
                     ATTSSGYAWSQARADHAPDVERVLAHCHEIVEATGLPVNADFEDGHARDLDGLGENVR
                     RCIETGIAGLSIEDSTGDAAQPLYDFDEAVARMRAARQAVDASGQDVVLVGRAECFLV
                     GHADALNESIKRLRAYAEAGADCLYAPGIGKPEDIRAIVEAVAPKPVNVLVGRAAPYT
                     LEDFAKLGVRRVSVGGALAGAAWGGFLRAARGLAEGRFDGFADNATHGELDALFTS"
     gene            12664..14745
                     /locus_tag="VFK00_01670"
     CDS             12664..14745
                     /locus_tag="VFK00_01670"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET7591697.1"
                     /translation="MLQQYGIWVALGCAIIAILYGVVSTGWIMKQSAGNERMREIAGA
                     IQEGARAYLNRQYGTIAIAGVVLFLLVGFFLNWATAIGFAIGAILSGATGYIGMNVSV
                     RANVRTAEAARHGIGPAMNVAFRGGAITGMLVVGLALLGVTGYFIILPHFGYTGEAAL
                     HALVGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTVVATMLLGGLAFAANPDAVLYPLVLGAASIIASIIGTF
                     FVRVKQGGSIMGALYKGVIVSAVLAAIAFWFITHSMFPGGLTGDDGATISGNALFYCS
                     LCGLILTGLIVWITEYYTGTDYKPVQHIAKASTTGHGTNIIAGLGVSMKATAWPVLAI
                     CAAIWTAYHFGGLYGIAIAVTAMLSMTGMIVALDAYGPITDNAGGIAEMAELPDEVRG
                     VTDPLDAVGNTTKAVTKGYAIGSAALAALVLFADYTHNLDAAGKAVAVASGVTYQPFA
                     FDLSDHMVIIGLLIGGLIPYLFGAMAMEAVGRAAGAVVEEVRRQFREIKGIMEGTGKP
                     KYDRAVDMLTKSAIKEMIIPSLLPVVVPILVGLILGPKALGGVLIGTIVTGLFVAISM
                     TAGGGAWDNAKKYIEDGNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIV
                     ALLIIPLLALH"
BASE COUNT         2439 a         5132 c         5013 g         2487 t
ORIGIN      
        1 gagtccgaaa cctgaactgc aattcatcgc tgaatcacta aggttcaaag gtttggacgt
       61 cttgcatcga tgaactgtcg tccacctgca agccgcccac acaagtcacc tgtgcacact
      121 gtcaaggaac tttgcggtgc gaaaccttcc ggtttcgcgt cgcgcgacat ttcgtctcgc
      181 gagccgccca ttgtagccag gtttcacgga ctgtcaacaa ctttggggaa agttcttgct
      241 gcccggggaa cccgcccgca accgatttcc gttgcgcggg ccgacaagga taccgggatc
      301 gtttcgcctt tggaagtgag gctgcgcgaa cggcgctaca ctgcggtttt ccgcctgcaa
      361 ggccacccgc gatgcgcccg ctttcctggc tgtcgatacc catcgttgcc gcgacgctgg
      421 catgtgccgc ttgcgccgct gcgccgccgg cgcccgtcgc gcctgccggc acgcccaccg
      481 cggtgcttaa cggccatcgc ttcagcgtgg aaatcgccga cacgccagcc cagcgtgagc
      541 acgggctgat ggatcgcacc tcgatgccgg gcgatcacgg catgctgttt gtttttcccg
      601 acgcggagcc tcgcacgttc tggatgaaga acaccctgat cccgctggac atcctgtact
      661 tcgacaatgc gcggcggctg gtctcggtgc aggaggacgt tccgccttgc cgagccgatc
      721 cgtgtccggt ctacccgagc atcgcgccgg ctcgttacgt gctggagctc aacgccggcg
      781 tggccgcgaa gctgggcgtc cgcaggggcg acgccatcac cttttcgggc cttccttccg
      841 gcacccagtg aaataccccg tcgtctccgt gcacgcacgt gaagttcatc ggcaggggca
      901 ccagccggcc cggcggctcg ctgtcatacc accgatcgtc gaagggaatg aattcgcgca
      961 ccctgctctc cttgtccaga tgacgctccc atgcaagcac gcccgcgtga acgagggatg
     1021 acccaaatgg ggcaggcatg tggcgaaccg gcccgggccg ggcgccgcaa tgggcgtagt
     1081 gatcaggcgg cgtagctgtg ccgtgatttt cgtcgccgag gggcgcgacg cgcgacccgt
     1141 gtcagtcgac ttcgatcatc tcgaagtcgt ccttggtgac gccgcaatcg gggcacgtcc
     1201 acgtgtccgg gatatcttcc cagcgggtgc cgggctcgat gccttcttcc ggcaatccgg
     1261 cttcttcgtc gtagatgaaa ccgcacacca cgcacatgaa cctgcgatag gcgacgacgg
     1321 ttgcggcggg agcattcatg gacaggatcg aggtgacgca agggacgata agccctattg
     1381 tcgcaagttg cgcgcctgca tgaaagcgaa cccttgacgc aagtcagccc gccgatgcca
     1441 caggaatcgc actttcgcag ccgcggcccc tccacaaggc ctgggacggg gctctacgcc
     1501 atcaccgacg gcccccgcga cgacctgttg caggtcgcgc gtgcggcgct cgaaggcggc
     1561 gcggcgatgc tgcagtaccg cgacaaaacc cgcgacggtg gccgccggct gcgcgaggcg
     1621 cgcgcgctgg cggatctttg cacatccttc ggcgttccgc tgatcgtcaa cgacgacgtc
     1681 gaactcgccc gttctagcgg cggctcgggc gtgcacctcg gcaaaggcga tggcaccatc
     1741 gcggatgcgc ggggcgccct cggcaccggc gcgatcgtcg ggatgtcttg ctacgactcg
     1801 ctcgaacgcg cgcgcgatgc cgccgcggag ggcgccgatt acctggcatt cggcgcgttc
     1861 ttcccgtcga tcaccaaacc cggcgcgcgc cacgccacgc ccgacttgct gcgcaaggcc
     1921 cgcgagttcg atcgtccgct ggtggcgatc ggcggcatta ccgccgaaaa tggcaggccg
     1981 ctgatctctg ccggcgcgga ttacctggcg gtggtctccg gcgtgttcgg tgcgaacgac
     2041 gtccgtgccg cggcacggcg ttacgcagag ctatttgacg cggatcaaag cgcataaccg
     2101 cggatggaac ggatttgcct gtccggacca gctttcatcc gcgtagatcc gcgtcggacg
     2161 cttttcctcg ctttcccacc aaccagcggc gaatcctttg atggccagca accacgaact
     2221 gttccagcgc gcccggcagg tcctccccgg cggcgtcgat tcgccggtgc gtgcgttcaa
     2281 atcggtcggc ggcgagccgt tcattgctgc ccgcgcggac ggcccgtacc tgtgggatgt
     2341 cgagggcaag cgctacatcg actatgtcgg ctcctgggga ccgatgatcg ccggccacaa
     2401 ccatccgcgc gtgcgcgaag ccgtggagcg agccgttcgc gacggcctct cgttcgggac
     2461 cccgtgtgcg gcggaggccg aactggccga ggcgatccgg gagctgattc cctcgctcga
     2521 gatgctgcgg ttcgtgaact cgggtaccga ggccacgatg tcggcgctgc ggctcgcgcg
     2581 tggagcaacc gggcgcgcgc ggttcgtgaa gttcgaaggc tgctaccacg gccacggcga
     2641 ttcgtttctg gtgaaggcgg gctcgggagc gctgacgttc ggcgttcccg actcgccggg
     2701 cgttccgaag gcgttggccg acctcacgat cacgctgccc tacaacgatg ccggagcggc
     2761 gcgcgcgctg ttcgaggaag ccggcaagca cattgcgtgc gtgatcgtgg aaccggtcgc
     2821 gggcaacatg aattgcatcc cgccgcagtc gggattcctc gagacgctgc gcgagctttg
     2881 cacccgcgac ggcgccttgc tgatcttcga cgaggtgatg acggggttcc gggttgccgc
     2941 cggcggggcg caagccctgt acggcgtgac gccggatctc acgaccctcg gcaaggtgat
     3001 cggcggcggg atgccggtcg gcgcgtatgg cgggcgccgg gacctgatgg aacaggtttc
     3061 gccggcgggg cccgtctacc aggccgggac tttgagcggg aatcccgtcg cgatggccgc
     3121 tgggctcgca atgctcgacc tggtccgcgg ggacggcttc cacgccgagc tggagcgcaa
     3181 gacccgtgtg ctcaccgagg ggctcgcggc ctgtgcccat gaggccggcg tgccattttc
     3241 ggtcaatcgg gtgggcggga tgttcgggtt gttcttcagc gcggggccgg tggaaacctt
     3301 cctgcaagcc acgtcctgcg acatcgcggc gttccggcgt ttcttccacg tgatgctcga
     3361 gcagggcgtg tatttcgcgc ccagtccctt cgaggccggg ttcatgtcga cggcgcacgg
     3421 cgataacgac atcgcacata cgctggaggc tgcgcgcgta gcgttcgcgg aagcggagcg
     3481 cgcgtagcgg cgaggaggat ccctggggcg gactcagtcc gccgcttcgc cagcaccctc
     3541 gaccgcagcc accgccacgg ctggccttgc ccgccctacc acgtgccgat gttgggcatc
     3601 gaggcccaag gctccgacgg cggcaacgga tcgccttcct gcagcagttc gatcgagatc
     3661 aggtcgggcg agcgcacgaa tgccatgtgg ccgtcgcgcg gcgggcggtt gatcgcgacg
     3721 cccatcgact gcaggtgcgc gcaggtcgcg tagatgtcgt cgacgcggaa ggcgaggtgt
     3781 ccgaagttgc gggccgagcc atagtcctcc tgcgaatccc agttccaggt cagctcgatt
     3841 tcggcatcgg ggctgtcggg ccccgacagg aataccagcg tgaacctgcc ttcgggcacg
     3901 tccctgcgcc gcgcctcgcg cagccccagc ccctcgcagt agaacctgag cgacgcatcc
     3961 aggtcgcgca cgcgcaccat gctgtgcagg tatttcatgc cctgtcctcc gaggccatcg
     4021 tcgtcggtta tcgtgggcac gttgtgccgg taccgcaagt caggagacga ttcgatggac
     4081 cccaagttcg acacgctcgc ggtgcacgcg ggcgcggagc ccgacgccgc gacgggcgcg
     4141 gtcgcgccgc cgatccagct ttcgaccacg ttcgtgcatg acccggcgcg cggcaccgac
     4201 gcctacagct accagcgcca ggacaatccg acccagcacc gcctcgaaac cgcgctggcc
     4261 gcgctggaag gcggcgagcg cgcgcttgcc ttcgcctccg gcatggccgc ggtggccgcc
     4321 gtcctggaca ccttgcagcg cggcgagcgg gtcgtggttc ccgaagactg ttacgtcgga
     4381 acccgcgggt atgtcggcga ggtcctaccg gaacgcggcg tggaggtggt gaccatcgac
     4441 accaccgacc tcgaggcggt agcccaggca tgcggcgacg gagcgtcgct gctgtgggcc
     4501 gagtccccgt cgaacccgcg gatgcggatc agcgacctgg ccgaactcgc gcggatcgcc
     4561 cacgccgccg gcgcgcgctt cgcctgcgac aacacgttcg caaccccggt gctgcaacgg
     4621 ccgctcgcgc tcggcgcgga catcgtgatg cactcgacca ccaaatactt cggcggccac
     4681 agcgacgtgc tgggcggcgc actggtattc gcgcgcaatg gcgatttcgc cgcgcggatc
     4741 gcgcagcggc gcgtgctgac cggctcgata ctcgccccgt tcagcgcctg gctgacgctg
     4801 cgcggctgcc gctcgctgcc ggcccggatg gcgcggcact gcgagaacgc gcgcaaggtc
     4861 gctgaatttc tcgcaggtca ccccgccatc gaacgcgtga accatccggg cttgcctgag
     4921 catccgggcc acgccattgc ctcgcggcag atgcgggatt tcggcgcgat gctgagcatc
     4981 gaattgcgcg gcggctacga cgcggctgtc gccgtcgcgc gcaacacgcg gattttcacc
     5041 aatgcgacgt cgctcggcgg ttgcgagtcg ctgatcgagc accgcgcatc ggcggagggg
     5101 gagcacaccc aggcgccgcc tgcgctggtg cggatatcgg tggggctcga agacgccggc
     5161 gacctgatca ccgacctcga ccaggcgctg tcgttttcat gaagatccag tgtcgcctgc
     5221 cgcgcccgca tcgtggttcg tcccgccggt tttccctggc ggagcgccga gccggaaccg
     5281 gcaggccggc gcaccgtgca gcatgcattg ggtgcgctgc accggccgcc cggtcgcggc
     5341 gcccatgaag gcgaggtcga acttgcagat gtcgggatgc gtccgggcca gcgaatggaa
     5401 cacgcagttc cacgcctcca cctccatgtg gccgtcggcg tcggcgacct gcgcctcgta
     5461 gcccagcgtg tcgagttgct cggccagcag gcgggccgcg tcgtcattgc tgccagccgt
     5521 cgcgacccgc gcggccgcgt cctcgccgag cttcgcaccc atgctcgaaa gcatggcctg
     5581 caccgcctga gcgcccgcgt gcgagtagag gtactcgagg acgccgccgg cgatcaaggc
     5641 atagttgcgc gggaacagtt cgcgtcccgc cggcagcagc acgaaacagg cgcgcgggcg
     5701 cccaccgctg ggggaggact caccgcgcgc cacgaagccc tgggccatca gtgcggacag
     5761 gtgctgcctg accgcgttgt gggtcacgcc gagggccttg cacaactctt caacgttcgc
     5821 gccctgcggc gccaacagca accgccgcag cagcttctgc tgggtggtgc cgagctgccg
     5881 cagggcgtcc gcgcgtcgca tccggtcagt gtgccttgtc ggggaactgc ttggcgatgg
     5941 caccggccag cgcatcggcg atcgtgtccg cgtgcttctg catgtgctcc caggtctggc
     6001 tttgctcggc ctcgggggca ttggccatca tttgctcgat ctgggtcttg tggtcgctga
     6061 cgtgcatcat ccagaggcca ttcaccgttt gctccggaag gttcggattg gcaccggcca
     6121 ggaacttcgc gatgtcgctg ccgttgctcg ccaggtcctg catcgcctgc tgctcccccg
     6181 ccttgttgcc cgcctgcacc gagtcggtga gattcttgac gccgccccag tgtccagcca
     6241 ggagcttgag gaactggtcc cccgccggct tgccgtagaa gctggcgacc gcgcccgcga
     6301 tctgtttggc gttggccacg gcgtcatcgg ccgcctgctg cgccttggcc tggttgccgg
     6361 cgtgcaccgc cagcgcgtaa tcgcgggtgg ccacgatgtg gccgtgcaac agcgagcgca
     6421 tggtcgcctg caattggggg gcggcgggcg tggatgcggc ggtcgccgcg ggatgggcga
     6481 acacgggcgg cgcggcgagc gccgcgccca aacccagcgc cagcgcacag gcaatcgtga
     6541 gcggtttcat gatgtatctc ctcgaggtct atcgccggac ggcgaccgac ttatcgtggg
     6601 cgatttccac atatagtaaa cctctatatg tgtaaagcaa cgatgcactc gaaggctgtc
     6661 gtgaaaagtc ggggcgagag cgggcgcatc gcgtgcccgg cccagcgact ggccaagcat
     6721 ggccggggcc cgccgccatg gatggctgct tgctgaagca tccgtggccg ccggctccgg
     6781 cgaagcagcg atcatgcacg atcgcaaact caagccttct ggaagcgcgg catcgcgtcc
     6841 cgcagttccc cgagccgttc cagcgggtcg gtgatctcca gcaggtgctg gcgttcggca
     6901 tcgcccaggg gcagcagctc ggcaagccgg aaacccaccc agctcgcgtt gtcgtactgc
     6961 tcgcgcgggc cgttgcgcca ggacagcccc gcctgttcgg ccagtcgttc caggatcgtc
     7021 ggcaacagtg aaaattcgac cgggaccggg acacgcggtt cgtccggcca gcggcgcacc
     7081 tcgccgatca ccagtccgtt gctgcggatg cgggtcttct ggacccggaa acgcgccccg
     7141 cccgtgacgc tgatgccgag caggccgtcg ggcagcgtat cgaaatcgat gatgcgcgcg
     7201 agggttccga tcgccgcggg cagcgccggc tcgccggcct cctggccctc catgatcagg
     7261 cacacgccga aggtgcctcc gctgcgcgcg cactcgcgca ccatgtcgat gtagcgcaat
     7321 tcgaagatcc gtagccccag cgtgctgccg gggaacagca cggcgttcag cgggaacagc
     7381 gggatctcgt ggcgcttcac ggctcgagtt tagccgccgc gcaatgcctc gagaaaccgg
     7441 cgcggcgcgt tttcgaaacc gccattcgac atgaacacga cgtggtcgcc agggcggatc
     7501 tcgctcacga gcgcctgcac caattcttcc accgagcgca ccgcacggcc ccgcccctcg
     7561 agcgacgccg tgacccgccc cgcgtcccag gaaagttccg gccgctgcag cagcagcacc
     7621 cggtcggcgt cggccagcga aggggccagt gccgcgacat gggcgccctg gcgcatcgaa
     7681 ttggaacgcg gctccagcgc cgccacgatc cgcgcgttgc cgaccctcgc gcgcaggccc
     7741 gccagcgtgg tcgcgatggc tgtcgggtgg tgggcgaagt cgtcatagac ctgcaccccg
     7801 ttcacgtcgc ccagcgattc catgcgccgc ttcacgctcg cgaaggtggc gaacgccggc
     7861 agcagggccg ccgggtcgat gcccgcggcc gtcgccgctg ccagcgcagc cagtgcgttc
     7921 atcacgttgt ggcgccccag caggctccag cgcacctcgc ctaccggctg gccccgccgg
     7981 gtcaccgcga acgcagaacc atccggctcg accagctgcg cccgccagtc gcccgcatcg
     8041 atcccgaagg tttcgaccgg tgtccagcag cccatcgcca gcacctcggc cagtcgcgca
     8101 tcctccgcgt tgacgaccag ccgcccattg cccggcacgg tgcgcacgag gtggtggaac
     8161 tggcgctgga tcgcggccac gtccggaaag atgtcggcgt ggtcgtattc gaggttgttg
     8221 aggatcgcga cgtcggggtg gtaatgcacg aacttcgagc gcttgtcgaa gaatgcggtg
     8281 tcgtattcgt cggcttccag aacgaatggg gtcgccgcag gcgacgcgtt atttccttct
     8341 cccttcggga gaaggggcgt ggaaacgccg ccttttcttt tgcgcgcagc gccggatgag
     8401 ggtacgggtc cgcgcaatct ggccgacacc ccgaaattgc ccggcacccc gccgaccagg
     8461 aaccccggcg ctgcgccggc cgactcgagc aaatgcgcga gcaggctcgt ggtcgtggtc
     8521 ttgccgtggg tgcccgcgac cgcgagcacc tggcgatcgc gcaacacctg ctcgcccagc
     8581 cactgcggcc cggatacgta cggaagcctt gcatccagca tgtattcgac cgccgggttg
     8641 ccgcgcgaca acgcattgcc gacgacgacg agatcgggcg ccggcttcag gtattccgcc
     8701 gaccagccct cgcgcagggt gatgccgagc gcgcgcaact ggtcgctcat cggcgggtac
     8761 acgttggcgt cggagccctc gacctcgacg ccgagttcgc gcgccagcgc cgcaacgcca
     8821 cccatgaacg tgccgcagat gccgaggatg tgtatgcgca tgccgattcg tccgaccctc
     8881 aggccagccc gatcacgctg gcggccacgg ccgtggcgac caggttcacg aacaccagca
     8941 gcaggaacac gagcacgccg ccggcgacag ggatttcgag tgaacgacgc aggatgccga
     9001 cccagaggca caactgccag atgcccaatg ccgcgatcac caccatcgcc aaggtctggg
     9061 gacccgacag cgccggcaaa tggggaggtt gtgacgtcaa ttgcttccgc agctcatcca
     9121 tcggcagcag cagcgtcagc aaattgctga cgacgtcgta aagcaggtac acgaccgcca
     9181 gggcggtggt cgtctgtacg aaacgctcgg acttgccgcg ccgacgcagc aacaggaata
     9241 ccgtcacgat gatcgcgagg ctgccgatga ccgccccggc ggccaatgcc ggctggacgc
     9301 catcgcggag gttgaacacc acctggacca cgccgcacgc caccagcaat ccgaccagca
     9361 gccgcggcga gtacggcatt tcctccgggc cgccccggaa caggcacaag tcgcggacgc
     9421 tggcgagaac cccgagcaag ttcacgcgtt ggcgcgggcg cgcagcgcgg cgagcagccg
     9481 ctcgccgatt acctcgatgt cccccactcc atcgatctgg gccagcagcc cgcgctccga
     9541 atagaaatcc gccaccggcg cggtctgcgc ggcgtacacc gcgaggcgct tgcggacggt
     9601 ttccgggttg tcgtcggcgc gatgctcctg ctcgaagcgg atctcgcagc gcttgatgat
     9661 gtcggcgtcg ggcacgttca acttcaccac cgcgtcgagg ggctggccga ggcgcgcgag
     9721 caaggcgtcg agcgcgccgg cttgcgcgag gttgcgcggg tagccgtcca ggatgaaccc
     9781 gggttcggtg tcgggctgtg cgaggcgctc ttccaggagg cccaggacga tgtcatcgga
     9841 taccagttga cccgactcca tcacggcctt ggccttgagg cccagcggtg ttccggcctt
     9901 gaccgccgca cgcagcaggt cgcccgtcga aatgtgggca acgccgagtt ccaccttcaa
     9961 ccgcttggcc tgggtaccct tgcccgatcc gggcgcgccg agcaatacga tgcgcatgcg
    10021 attcctctta gctatctgcc gggaaagtgc tgtcccgcac cttcccggcg cgccggatca
    10081 agcgctgctc gatccgcggc gggccatcca ttgcccaccc ggcaggccgt gacggcctgt
    10141 cggcccgcgg agcggcggcg ccttctttaa caaattccgc tttgggagtt acgctagcgg
    10201 ctgcgcagct tccccgtcaa cgccgcgcca ggaatggaaa cccgcatgac cgcgaccccg
    10261 aaacccacag gcaggctatt gtacgcgcag tccggcggcg tcaccgccgt gatcaatgcg
    10321 accgccgcgg cggtgatcga ggcctgccgc aaacaccagg tcgcggtcta cgccgcgcgc
    10381 aacggcatcc tcggcgcgtt gcgcgaggac ctgatcgata catcgaagga atcgccgtcc
    10441 gcgatcgccg cgctgaaaca cacgcccggc ggcgccttcg gttcgtgccg ggtcaaactg
    10501 aaatcgctgg aagacgaccg cgcgaagtac gaacgcctga tcgatgtgtt ccgcgcgcac
    10561 gacatccggt ggttcctcta caacggcggc aacgattcgg cggacaccgc actgaagctg
    10621 tccaagctcg gcaaggcggc gggctacgac atccgctgca tcggggtgcc caagacggtc
    10681 gacaacgacc tcgcggtcac cgactgctgt ccgggcttcg gctcggtcgc gaaatacacc
    10741 gcggtgtcct tgcgcgaatg cgcgctggac gtgatgtcga tgatggacac ctcgaccaag
    10801 gtgttcgtga tggaggtgat gggtcggcac gccggctgga tcgcggccgc cgctgggctt
    10861 gcaggcgcga ggccggacga cgcgccgcac atcatcctgt ttcccgaact ggaattcgac
    10921 gaggaaaaat tcctcgcgcg ggtgcgcgaa accgtggacc gggcgggttg ctgcgtgatc
    10981 gccgcgagcg aaggcatcaa gtacgcggac ggcaagttcg tcgccgacgc gcacgccggg
    11041 accgacgcct tcggccacac ccaactcggc ggggtcgcgc ccgcactcgc cgggatggtg
    11101 cgcggcaagt tgaagctcaa gacccattgg gcgatgccgg actacctgca gcgttccgcc
    11161 cggcacattg cctcgaagac cgatctcgac caggcgctcg cggtcggcac ggcggcggtg
    11221 gaactggcgc tcgccggcga gaacgcggtg atgccggtga tccggcgcac ttccgatgca
    11281 ccgtatcgct ggaaaatcga cacggcgaag ctcgaccaga ttgcgaatcg cgagaagaaa
    11341 ctgccgcgtt ccttcatcgc gaaggacggt ttccacatca ccgccgccgc gcgcaaatac
    11401 ctctcgcccc tgatccgcgg cgaagcgccg ccgccgtacg gcaaggatgg agttcccgga
    11461 tacgtcacgc tgaagaacgt caccgtcaag aagaagttgg gatcgttcga actgtaggtt
    11521 gggctgggcg gcttctcgcg gagcccaaca gcgccgagtc gcagcacatt gggcttcatc
    11581 ctcgacaagc gcggattgaa cccaacttgg ggtgtttcat ccggccgagc ccatcgacgc
    11641 cgaacgacca tgttgggctt catccgcgaa aaacgcggat tcaacccaac ctacgacgta
    11701 aacagggcat ccaattcgcc gtgggtcgca ttgtccgcga agccgtcgaa gcggccttcg
    11761 gccagaccac gagcggcgcg caggaatccg ccccatgcgg cgccggccaa cgcgccaccg
    11821 acgctgacgc ggcgcacgcc aagcttcgcg aaatcttcaa gcgtgtacgg tgccgcacgg
    11881 cccaccagca cgttgacggg tttgggcgcg acagcctcga cgatcgcacg gatgtcttcc
    11941 ggcttgccaa tgcccggcgc gtacaagcag tcggcgccgg cctcggcgta ggcgcgcaaa
    12001 cgcttgatcg actcgttcaa agcatccgcg tggccgacga gaaaacattc ggcgcggccg
    12061 accagcacca cgtcctggcc cgaggcgtcg acggcctggc gggcggcgcg catccgcgcg
    12121 acggcctcgt cgaaatcgta cagcggctgc gcggcgtcgc cggtcgaatc ctcgatcgac
    12181 aagccggcga tgccggtctc gatgcagcgg cgcacgtttt cccccaagcc atcgaggtcg
    12241 cgcgcatggc cgtcctcgaa atccgcattg accggaagcc cggtggcctc cacgatctcg
    12301 tggcaatgcg ccagcacacg ctccacgtcc ggcgcatggt ccgccctggc ttgcgaccag
    12361 gcgtaacccg aactggtggt cgccagcgcc cggaacccga gcctttccag cgcgtgcgcg
    12421 gaacccgcgt cccacgggtt cgggatcagg aagcagcctg tttcatgcag cttgcggaag
    12481 gcggcgcggc gcgcggcaaa gcgatgggcg ttcatgcagg cttcctcgag tgcctcggac
    12541 aaggcgaaca ggcattctag gactgccgcg gcacgccatg cccctggagg ctggtcccgc
    12601 ggcatgccgc agctatactg aggggattgc ccgttgcggg ttccgctgaa aggggttttt
    12661 ccgatgctgc agcagtacgg catatgggtc gcgctgggtt gcgcgatcat cgcaattctc
    12721 tacggtgtcg tctcgaccgg ctggatcatg aagcagtcgg ccggcaacga gcgcatgcgc
    12781 gaaatcgcgg gcgccatcca ggaaggcgca cgcgcctatc tgaaccgcca gtacggcacc
    12841 atcgccatcg ccggcgtcgt gctgttcctg ttggtcggct tcttcctcaa ctgggccacc
    12901 gcgatcgggt tcgcgatcgg cgcgatcctg tccggcgcca ccggctacat cggcatgaac
    12961 gtctcggtgc gcgccaacgt gcgcactgcg gaggcggcgc gccacggcat cggcccggcc
    13021 atgaacgtgg cgtttcgcgg cggcgccatc accggcatgc tggtggtggg cctggcgctg
    13081 ctgggggtca ccggctattt cataatcctg ccgcacttcg gctacaccgg cgaggcggcg
    13141 ctgcacgcgc tggtcggcct ggccttcggt tcttcgctga tctccatctt cgcgcgcctg
    13201 ggcggcggca tcttcaccaa aggcgccgat gtcggcgcgg acctcgtggg caaggtcgag
    13261 gctggcattc cggaagacga cccgcgcaac ccggcggtga tcgccgacaa cgtcggcgac
    13321 aacgtgggcg actgcgccgg catggccgcc gacctgttcg agacctacgc ggtgacggtg
    13381 gtcgccacca tgcttctggg cgggctggcg ttcgccgcca atccggacgc tgtgctgtac
    13441 ccgctggtcc tcggcgcggc ctcgatcatc gcctcgatca tcggcacctt cttcgtgcgg
    13501 gtgaaacagg gcggctcgat catgggcgcc ctgtacaagg gcgtgatcgt ctcggcggtg
    13561 ctggcggcca tcgcgttctg gttcatcacc cactcgatgt tcccgggcgg actgaccggc
    13621 gacgacggtg ccacgatctc cggcaacgcg ctgttctact gctcgttgtg cggactgatc
    13681 ctgaccggcc tgatcgtgtg gatcaccgag tactacaccg gcaccgatta caagccggtg
    13741 cagcacatcg ccaaggcctc cacaaccggc catgggacca acatcatcgc cggcctcggc
    13801 gtctcgatga aggccacggc gtggccggtg ctggcaatct gcgcggcgat ctggaccgct
    13861 taccatttcg gcggcctgta cggtatcgcc atcgccgtca ccgcgatgct gtcgatgacc
    13921 ggcatgatcg tggcgctgga cgcttacggc cccatcaccg acaacgccgg cggcatcgcc
    13981 gagatggccg agctgccgga cgaggtgcgc ggcgtcaccg atccgctgga tgcggtgggc
    14041 aataccacga aggcggtgac caagggctac gccatcggct ccgcggccct cgcggcgctg
    14101 gtgctgtttg ccgattacac gcacaacctc gacgcggccg gcaaggccgt agccgtggcg
    14161 agcggcgtca cctaccagcc attcgccttc gacctgtccg accacatggt gatcatcggc
    14221 ctcctgatcg gcggcctgat tccgtacctg ttcggggcga tggcgatgga agcggtcggc
    14281 cgcgccgctg gcgcggtggt cgaggaggtc cgccgccagt tccgcgagat caagggcatc
    14341 atggaaggca ccggcaagcc caagtacgac cgcgcagtgg acatgctgac caagtcggca
    14401 atcaaggaaa tgatcatccc gtccctgctg cccgtggtcg tgccgatcct ggtcggcctg
    14461 atcctgggcc cgaaggcact gggtggcgtc ctgatcggca ccatcgtcac gggcctgttc
    14521 gtcgccatct cgatgacagc gggcggcggc gcctgggaca acgccaagaa atacatcgag
    14581 gacggcaact tcggcggcaa gggttcggaa gcgcacaagg ctgcggtcac cggcgatacc
    14641 gtgggcgatc cgtacaagga caccgcgggt ccggccgtga acccgctgat caagatcatc
    14701 aacatcgtgg cgctgttgat catcccgctg ctcgcgctgc actgaccgcc gtcagacgct
    14761 gtacgaaaag ccccgccgcg tgcggggctt tttgtcgggg cccccgaacc gttgaagcgc
    14821 gggcgcggtt gggacatgat ggcgccggtt ttccctcggg gacgggaaag tgtcgcgcga
    14881 ccggtgggca tcgtcggtgg ggatcggcgg ccgtggcatg gcgtggaccg catgctgatt
    14941 gccgcgctcg cgctcgcctt cgccattgcg gtcgcatggc tgttcaaccg gctggtgcgc
    15001 ctgcgcaacc aggtgcgggt cgcgtggagc gacgtggacg tgcaactgca gcgccgccac
    15061 gacctggtgc c
//
LOCUS       DASSNH010000045         9704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_318785, whole genome shotgun sequence.
ACCESSION   DASSNH010000045 DASSNH010000000
VERSION     DASSNH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318785"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3377)
                     /gene="dnaE"
                     /locus_tag="VFK00_01675"
     CDS             complement(<1..3377)
                     /gene="dnaE"
                     /locus_tag="VFK00_01675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7591698.1"
                     /translation="MPIDFIHLRVHSEYSLRDSTIRIPAKPEYGDPAKARQANLVSRA
                     VELGLPALALTDDSNLFAQIKFYRAAERAGIKPIVGCDLWLADPEDPSRPGRLTLLCR
                     DHAGYLNLTRLISRAWLEGQHAGRAQLQAEWLDGAVSGLLAIAGRDSGFGRLAAAGRE
                     HDARSNIDALRRHFGDGLYLELTRTGRSGEDAFNAQALQLAAGLDLPVIASNDVRFLD
                     ADDFEAHEARVCIQQGYQLQDPRRPRDYSAEQWLKPADAMAAAFADLPEVLENTVELA
                     KRCNLELSFGTYYLPAFPVPANHTLESWIGDQARAGLEQRLAESGIAAGHTREDYETR
                     LANELDVIGKMGFAGYFLIVSDFIRWAKAEDIPVGPGRGSGAGSVVAWSLRITDLDPL
                     QFGLLFERFLNPERVSMPDFDVDFCMERRDEVIAYVAQKYGRDQVSQIITYGSMAAKA
                     VLRDCGRVLGMPYGQVDRIAKLIPRMPLDLTLSDALGLSEKSRKEPDRVVREFCELYQ
                     QDDDARTLIDLALKLEGLARNAGKHAGGVVIAPTPLTDFAPLYSEPGGGGVVTQYDKN
                     DVETVGLVKFDFLGLRTLTILDWAVKAINARRAASGEAPLDLAHLPLDDAEVYRLFRE
                     ARTVAVFQFEGAGMQRLLKDAKPDCLEDLIALNSLFRPGPMDLIPSYVARKHGREPVT
                     YPDPRVEPILKETFGIMVYQEQVMQMAQIVGGYSLGGADLLRRAMGKKVPAEMAKHRS
                     IFRDGAMQNGVPERQADQIFDQMEKFAGYGFNKSHAAAYSLVAYQTAWLKTHYPAEFL
                     AATMSADMDATDKLVLFIEDARAMGIAVLPPDVNASRHQFVANDDVDRQANPDARGTI
                     RYGLGAIKGVGRGVCEAIVAARESGGPFRDLADFCARMDSGRLNKRVFEALIQSGAMD
                     ALGANRATLTAQLPEAVKAAEQSLRDREAGQNDMFGAALAPVATVSLGTVAQPSWSLE
                     KRLAGERATLGHYFSGHPTDPWREVLAQLATCPIGDIAQHYEPPPPRGGDDENRWRRA
                     PETPWAVAGMIVKMRRRGDSAAAVRIEDWSGAIEINFFREALQQYGPLLQPDAILVVE
                     GGLALDDFSGELALRARRAWTLDEACETGARVLRVGV"
     gene            complement(3559..4224)
                     /gene="rnhB"
                     /locus_tag="VFK00_01680"
     CDS             complement(3559..4224)
                     /gene="rnhB"
                     /locus_tag="VFK00_01680"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7591699.1"
                     /translation="MTLVIDNGSDFATLPRHKLRRWPDVADLAGVDEAGCAPLAGPVV
                     VAAVILDPARRINGLDDSKVLPEPTREKLYARIVERARAWSVVAVEVAEIDRINIYHA
                     RMAGMLRALERLACAPAFALIDGNRLPRPLPCPARAVVDGDALAPAISAASIIAKVTR
                     DRMMRELDTHWPGYGFAQHKGYPTPAHCEALRRLGPCPHHRRSFAPVRAALEQELPLA
                     LPA"
     gene            complement(4234..5541)
                     /gene="lpxB"
                     /locus_tag="VFK00_01685"
     CDS             complement(4234..5541)
                     /gene="lpxB"
                     /locus_tag="VFK00_01685"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET7591700.1"
                     /translation="MTAKTIPHGEIEDRSDAGPRSPAPGPVFALVAGEDSGDQLGADL
                     IEALRARFPDACFVGVGGPRMRAAGFDAWHEISALSVMGLAEVLRHLPRLLRLRASLA
                     KRMITLKPALFIGIDAPDFNLGLERKLKQAGIRTAHYVSPSVWAWREGRAARIGHSAD
                     CVLCLFPMEPAIYARHGGNADFVGHPLADRFPLDPDRAAARARLGLPPDAPVLAVLPG
                     SRLSEIHQLGATFIEAARRVAENIPELRVVIPAANVGCRREIETILKSAGPGTGDQGP
                     EAGPDTMSGPRSAVPGPVRLLEGHAHDAMVAADVVLVASGTATLETLLAKRPMVVAYR
                     IAPLTYALVKGLGMLKTDTYALPNILAGRKVVPELMQADCTPDRLADAVTALFHSPER
                     RDEQVREFGRLHLALRTEGGAAASAARVIGRMIGQWDAAARDA"
     gene            complement(5677..6465)
                     /gene="lpxA"
                     /locus_tag="VFK00_01690"
     CDS             complement(5677..6465)
                     /gene="lpxA"
                     /locus_tag="VFK00_01690"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET7591701.1"
                     /translation="MTPASANIHPTAIIDPAAKLADDVTVAPYAVIGAEVEIRSGSTV
                     GAHALIKGPTRIGRNNRIGAFGSLGGDPQDKKYQGERSELVIGDGNTFHEFVTVSRGT
                     REGGGVTRIGDDNWVMAYAHIAHDCIVGSHTILANNATLAGHVEVGDHVVLGGFVGVH
                     QFCKIGAHAFAAMSSAINRDVPPFIYVAGQFAVPRGVNAEGLKRRGFDATRISAIKRA
                     YRTLYLSHKTLDEARTALARQAETSDDVRAFVEFMDRSERALVR"
     gene            complement(6462..6929)
                     /gene="fabZ"
                     /locus_tag="VFK00_01695"
     CDS             complement(6462..6929)
                     /gene="fabZ"
                     /locus_tag="VFK00_01695"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7591702.1"
                     /translation="MNKTAAPVKLPVDAEGIAAILPHRYPFLLVDRVIELVPGKSIVA
                     IKNVTMNEPFFQGHFPGHPVMPGVLIVEALAQASGLLIGLSGTLTNGDGLIFYLAKVD
                     NARFSKPVVPGDQLRLEVQLKRQIRGMGIFTACAVVDGKPAATCDLMCAESSR"
     gene            complement(6929..7978)
                     /gene="lpxD"
                     /locus_tag="VFK00_01700"
     CDS             complement(6929..7978)
                     /gene="lpxD"
                     /locus_tag="VFK00_01700"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET7591703.1"
                     /translation="MERPTGYRIDELAQRFGLELRGDGTGVVRGVAPLARAAGDKLAF
                     LANGRYASDLAGTGAGVVVLSAEHADASPVPALIARDPYLAYARIASLFERASADVPG
                     IHPTAVIADGAAVDSGATVGPHCVVEAGAAVEAGAVLGPHCVIGGDCRVGAQSRLAAN
                     VVLVTRVTLGRRVVVHPGAVLGADGFGIARSEEGWVNVPQLGGVRIGDDCEIGANTTI
                     DRGALEDTVLEEDVRLDNQIQIAHNVVIGAHTALAGCAAVAGSARIGRDCLIGGGVGV
                     LGHLEVADRVTITAMSLVTHSIRAPGVYSSGAPIEENRAWRRNAARVRQLDRMARRIA
                     ALEKQVESLTKEKRS"
     gene            complement(8130..>9704)
                     /gene="bamA"
                     /locus_tag="VFK00_01705"
     CDS             complement(8130..>9704)
                     /gene="bamA"
                     /locus_tag="VFK00_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET7591704.1"
                     /translation="PEDALRQVLRIQPGEIFNRHEIELSSDAITDVLSNIGYAFAKVQ
                     PIPKVDESSHTVDLTFYITPGPRVYVRRINFKGNTQTQGQILRREMRQYEGAWYSQAA
                     IDRSKVRLQRLGYFKDVTVDTVKVPGTADEVDLNVSVTEESSGQFQFGVGYSQVSGII
                     LSTSIANNNFLGTGNRISATFSKSLFLKQYQVSFFDPYLTDSGIGIGYNASVLKLNQA
                     EQNIASYLSDTDSFSTYLSFPISETDSINAGIGINKTKLDLIPGFTPQVFFDQINKVG
                     HYTMHNTPLTLSYSHDTLNAFFKPTRGGLQQISAEIALPGATVPYYKLTAATSDYFPL
                     PFGFVGHVSGNLGYGKVYGSNDISVQYADSTGRIRTMSFPFFENFYAGGVQDVRGFED
                     NTLGPRICSGLIGPDGKADPTAISSNGNCLGGTFYRPQPVGGAFKVKGSAELVLPFFK
                     DNDKARISLFADMGNVYSSYAAFDASDLRASVGISLQWIAPVGPIVINLAEPIRDKPE
                     DKPFEERLQFYFGRTF"
BASE COUNT         1522 a         3161 c         3385 g         1636 t
ORIGIN      
        1 ttcacgccga cccgcaagac gcgggcgccg gtctcgcagg cttcgtcgag cgtccaggca
       61 cggcgcgcgc gcagcgcaag ttcaccgctg aaatcatcga gcgccagacc gccctcgacc
      121 accaggatgg cgtccggttg caacagcggg ccgtactgct gcagcgcctc gcggaagaaa
      181 ttgatttcga tcgcgccgct ccagtcttcg atccgcacgg cggcggcgct gtcgccgcgc
      241 cggcgcatct tgacgatcat cccggccacg gcccacggcg tttccggtgc gcggcgccag
      301 cgattctcgt cgtcgccgcc acgcggcgga ggcggctcgt aatgctgcgc gatgtcgccg
      361 atcgggcagg tggcgagttg cgctagcacc tcgcgccacg ggtccgtggg atggccggaa
      421 aaataatgac ccagcgtcgc gcgttcgcca gcgaggcgct tttccagcga ccacgacggt
      481 tgcgcaaccg tgccgagtga aaccgtggcg accggggcca gcgccgcgcc gaacatgtcg
      541 ttctggccgg cctcgcgatc gcgcaggctc tgttcggcgg ccttgaccgc ctcgggaagt
      601 tgcgcggtga gggtggcgcg gttggcgccc agcgcatcca ttgcacccga ctggatcagg
      661 gcttcgaaca cccgcttgtt gagcctgccg gaatccatgc gcgcgcaaaa atcggcgagg
      721 tcgcggaatg gacccccgct ctcgcgtgcg gcgacgatgg cttcgcacac gccacggccg
      781 acacccttga tcgcgccgag gccatagcgg atggtgcccc gtgcgtcggg attcgcctgg
      841 cggtcgacat cgtcgttggc gacgaactgg tggcgggagg cgttgacgtc gggcggcagc
      901 acggcgatgc ccatcgcgcg cgcgtcctcg atgaacagca ccagcttgtc ggtggcgtcc
      961 atgtccgccg acatcgtggc ggcgaggaat tcggcggggt aatgggtctt cagccacgcc
     1021 gtctggtagg cgaccagcga atacgccgcc gcatgcgact tgttgaagcc gtagcccgcg
     1081 aacttctcca tctggtcgaa gatctggtcg gcctggcgtt cgggcacgcc gttctgcatc
     1141 gcgccgtcgc ggaagatcga ccgatgcttg gccatctcgg ccggcacctt cttgcccatc
     1201 gcgcggcgca gcaggtcggc gccgcccagc gaatagccgc cgacgatctg cgccatctgc
     1261 atcacctgct cctggtagac catgatgccg aaggtctctt tcaggatcgg ttccacgcgc
     1321 ggatcggggt aggtcaccgg ctcgcgccca tgcttgcgcg cgacgtagct cgggatcagg
     1381 tccatcggcc ccggccggaa cagcgaattc agcgcgatca aatcctcgag gcagtcgggc
     1441 ttcgcgtcct tgagcaggcg ttgcatgccc gcgccttcga actggaacac ggcgaccgtg
     1501 cgcgcctcgc ggaacaggcg atagacctcg gcgtcgtcca gcggcaaatg cgcgaggtcc
     1561 agcggcgctt cgcccgacgc tgcacgccgt gcattgatcg ccttcaccgc ccagtcgagg
     1621 atcgtgagcg tgcgcaggcc caggaagtcg aatttcacaa gtccgaccgt ctcgacgtcg
     1681 ttcttgtcgt attgcgtcac cacgccgccg ccgccgggtt ccgagtacag cggcgcgaaa
     1741 tcggtgagcg gcgtcggggc gatcaccacg ccgccggcgt gcttgccggc attgcgtgcg
     1801 aggccttcga gtttcagggc gaggtcgatc agggtgcgcg catcgtcgtc ctgctggtac
     1861 aactcgcaga actcgcgcac cacgcggtcg ggttccttcc tcgatttttc gctgaggccc
     1921 agcgcatcgg agagcgtgag gtccagcggc atgcgcggga tcagcttggc gatccggtcg
     1981 acctggccat aaggcatccc gagtacgcgg ccgcaatcgc gcagcaccgc cttcgcggcc
     2041 atcgagccgt aggtgatgat ctggctaacc tggtcgcggc cgtacttctg cgcgacgtag
     2101 gcgatcacct cgtcgcggcg ctccatgcag aaatcgacgt cgaagtccgg catcgacacg
     2161 cgttcggggt tgaggaagcg ttcgaacagc aacccgaact gcagaggatc caggtcggtg
     2221 atccggaggc tccatgccac gaccgaaccc gcgcccgagc cgcggccggg ccccaccggg
     2281 atgtcctcgg ccttggccca gcggatgaag tcggagacga tgaggaagta gccggcgaaa
     2341 cccatcttgc cgatcacgtc cagctcgttc gcgaggcgtg tctcgtagtc ttcgcgggta
     2401 tggcccgcgg cgatgccgga ttcggccaat cgctgttcca gcccggcgcg cgcctggtcg
     2461 ccgatccagg attccagcgt gtggttcgcc ggaaccggga acgcgggcag gtagtacgtg
     2521 ccgaaggaaa gctcgaggtt gcagcgcttc gccagttcga ccgtgttctc gagcacctcg
     2581 gggagatcgg cgaacgccgc ggccatcgca tcggccggct tcaaccactg ctcggcggag
     2641 tagtcgcgcg gcctgcgcgg gtcctgcaac tggtagccct gctggatgca gacccgcgcc
     2701 tcgtgcgcct cgaaatcgtc ggcatcgagg aagcgcacgt cgttgctggc gatgaccgga
     2761 aggtccagtc cggcggcgag ctgcagcgcc tgggcattga aggcgtcttc gccgctgcga
     2821 ccggtgcggg tgagttccag gtacaggccg tcgccgaagt ggcggcgcag ggcatcgatg
     2881 ttggagcggg cgtcgtgttc gcgtcccgcc gcggccagcc gtccgaagcc gctgtcgcgc
     2941 cccgcgatcg cgagcagccc ggaaaccgcg ccgtccagcc actcggcctg caactgcgca
     3001 cgtcccgcgt gctggccctc cagccatgcg cgcgagatca gccgtgtcag gttcaggtac
     3061 cccgcatggt cgcggcacag cagcgtcagc ctgccgggcc gcgatggatc ctcgggatcc
     3121 gcgagccaaa ggtcgcagcc cacgatcggc ttgatgccgg cgcgttcggc agcgcggtag
     3181 aacttgatct gcgcgaacag gttggagtcg tcggtgagcg ccagcgccgg caggcccagt
     3241 tcgaccgcgc ggctgaccag gttcgcctgc cgggccttgg ccggatcgcc gtactcgggt
     3301 tttgccggga tgcggatggt cgagtcccgc aacgagtatt cgctgtggac ccgcagatgg
     3361 atgaagtcga tcggcatggc ggtgcgcgtg gtatccgcgc aagcgtattg ccggcaggcg
     3421 ggggcggcaa gcttgacagg tcgtatatcg gccggaggcg ccactcctag gccgctcaag
     3481 cccgaagggt ggagccggca ttgtccgagc gtgatgatgc cggttccgtc gctgcgctcc
     3541 ttggaccggc ctgcaacgct atgccggcaa cgccagcgga agttcctgtt cgagcgccgc
     3601 ccgcaccggc gcgaaactgc ggcggtggtg gggacagggg ccgagcctgc gcagcgcctc
     3661 gcagtgcgcc ggagtcggat aacccttgtg ctgggcgaaa ccgtagcccg gccagtgcgt
     3721 atccagctcg cgcatcatcc ggtcgcgggt gaccttggcg atgatcgagg ctgcgctgat
     3781 ggcgggcgcc agtgcatcgc catccacgac ggctcgcgcg ggacacggga gtggacgggg
     3841 caggcgattg ccgtcgatca gcgcgaacgc cggcgcgcac gccagccgtt ccagcgcacg
     3901 cagcatgccg gccattcgcg cgtgatagat gttgatgcgg tcgatttccg cgacctccac
     3961 cgcgacgacg ctccatgcgc gcgcccgctc gacgatgcgc gcgtacaact tctcgcgcgt
     4021 gggctccgga agcaccttgg aatcgtccag cccgttgatg cgccgcgcgg gatcgaggat
     4081 caccgcggcg accacgacgg gacccgccag cggcgcgcag ccggcttcgt cgacgcctgc
     4141 gaggtccgcc acgtccggcc agcgcctgag cttgtgccgc ggcaaggtcg cgaagtcgga
     4201 accgttgtca atcaccaggg tcatgcaggt cgatcatgcg tctcgtgcgg cggcatccca
     4261 ttgcccgatc atccggccga tcacgcgtgc cgcggaggcc gcggccccgc cttcggtccg
     4321 cagggcaaga tggaggcgtc cgaattcacg aacctgttcg tcgcgtcttt ccggcgagtg
     4381 gaacagtgcc gtgacggcat cggcgagtct atcgggtgtg cagtcggcct gcatcagctc
     4441 ggggacgacc ttgcggcccg cgaggatgtt gggcagcgca taggtgtcgg tcttcagcat
     4501 cccgagtccc ttcacgagcg cgtaggtgag cggcgcgatc cggtaggcca ccaccatcgg
     4561 ccgcttcgcg agcaaggtct ccagcgtggc cgtgccggag gcgaccagca ccacgtcggc
     4621 ggcgaccatg gcgtcgtggg cgtggccttc caggagacgg acgggaccgg ggaccgcgga
     4681 tcggggaccg gacatcgtgt cggggccggc ttctggtccc tggtccccgg tccccggtcc
     4741 cgctgatttc aagatcgttt caatttcgcg gcggcacccg acgttcgctg cgggaatcac
     4801 aacccgcaat tccggaatgt tttctgccac ccggcgcgcc gcttcgatga aggtcgcgcc
     4861 cagctggtgg atctcggaga gccggctgcc tggcaggacc gcgagcaccg gcgcatccgg
     4921 gggcaggccg agccgggcgc gcgccgcggc gcggtccggg tccagcggga aacggtcggc
     4981 gagtggatgc ccgacgaagt ccgcgttgcc gccatggcgc gcgtagatcg cgggctccat
     5041 cgggaacagg cacagcacgc aatcagccga atgtccgatc cttgccgcgc gcccttcccg
     5101 ccaggcccag accgaggggc tgacgtagtg cgcggtacgg atgcccgcct gcttcagttt
     5161 ccgctcgagg ccaaggttga agtcgggagc gtcgatgccg atgaacaggg caggcttcag
     5221 cgtgatcatc cgcttcgcca gggaggcgcg caggcgcaac aggcggggca ggtggcgcag
     5281 cacctcggcg agccccatca ccgacaacgc cgaaatctcg tgccaggcgt cgaaccccgc
     5341 cgcgcgcatg cgcgggccgc cgacaccgac gaagcacgca tccgggaatc gcgcgcgcaa
     5401 cgcctcgatc aggtccgcgc cgagctggtc gccggagtct tcgccggcga cgagggcgaa
     5461 gacgggaccg ggggccgggg accggggacc cgcgtctgac cgatcctcga tttcgccgtg
     5521 gggaatggtc ttcgccgtca tgcgaaataa cgaaacagga tgccgggtgt tgtgacaagg
     5581 ctaatcggat tggtgacgtg ccgcgtgttg tacatcaagc gtgcagacga ccagtgctat
     5641 tcccggtccc cggtccctga gccccggtcc cggctctcag cgcaccaatg cccgctcgct
     5701 gcggtccatg aactcgacga aggcgcgcac gtcgtcactg gtttcggcct gccgggcaag
     5761 cgcggtgcgc gcttcgtcca gcgtcttgtg cgagaggtag agggtgcggt aggcgcgttt
     5821 gatcgcggag atgcgcgtgg catcgaagcc gcggcgcttg aggccttcgg cgttaacgcc
     5881 gcgcggcacg gcgaactgcc ctgcgacgta gatgaacggg gggacgtcgc gattgatcgc
     5941 cgaagacatc gcggcgaacg catgcgcgcc gatcttgcag aactggtgca cgccaacgaa
     6001 gccgccgagc accacgtggt cgccgacctc gacgtggccc gcgagggtcg cgttgttggc
     6061 gaggatggtg tggctgccga cgatgcagtc gtgggcgatg tgtgcgtacg ccatcaccca
     6121 gttgtcgtcg ccgatgcggg tgacaccacc gccctcgcgg gtgccgcggc tgacggtcac
     6181 gaattcgtgg aaggtattgc cgtcgccgat taccagttcg ctgcgctcgc cctggtactt
     6241 cttgtcctgc gggtcgccgc cgagcgatcc gaaggcgccg atgcggttgt tgcgcccgat
     6301 gcgggtcggt cccttgatca gcgcgtgggc gccgaccgtg ctgccggacc ggatttcgac
     6361 ttccgcgccg atgacggcat agggcgcgac cgtcacgtcg tcggcgagct tcgctgcggg
     6421 atcaatgatc gccgtcggat ggatgtttgc gctggccggt gtcaccgact gctttccgcg
     6481 cacatcaggt cgcaggtggc cgcgggcttg ccgtccacga ccgcgcacgc ggtgaaaatg
     6541 cccatcccgc ggatctgacg cttgagttgc acctcgaggc gcaactggtc gcccggcacg
     6601 acgggcttcg agaaccgcgc gttgtcgacc ttggcgaggt agaagatcag accgtcgccg
     6661 ttggtgaggg tgccggacaa tccgatcagc aagccggatg cctgcgccag ggcttcgacg
     6721 atcagcacgc cgggcatcac cggatgtccg gggaagtggc cctggaaaaa cggctcgttc
     6781 atcgtgacgt tcttgatcgc gacgatgctc ttgcccggca cgagttcgat cacccggtcc
     6841 accagcagga acgggtagcg atggggcagg atggccgcga tcccttcggc atcgacggga
     6901 agcttgacgg gggcggcggt cttgttcatc aactgcgttt ttccttggtc agcgattcca
     6961 cttgtttttc cagcgcggcg atgcgccgcg ccatcctgtc gagctggcgc acgcgcgccg
     7021 cgttgcggcg ccaggcacgg ttctcctcga tcggtgcgcc tgaagagtac acgccgggcg
     7081 cgcggatcga gtgggtcacc aggctcatcg cggtgatggt aacgcgatcg gccacttcga
     7141 ggtgccccag tacccccacg ccgccgccga tcaggcaatc ccgcccaatc cgcgcgctgc
     7201 cggccaccgc cgcgcagccg gccagcgcgg tgtgcgcacc gatgacgacg ttgtgggcga
     7261 tctggatctg gttgtcgagg cggacgtcct cctcgagcac ggtgtcctcg agcgcaccgc
     7321 gatcgatcgt ggtattggcg ccgatctcgc aatcgtcgcc gatgcggaca ccgccgagtt
     7381 gcggcacatt gacccagcct tcctcgctgc gcgcaatgcc gaagccgtcg gcccccagca
     7441 ccgcgccggg atggaccacg acgcgcctgc ccagcgtgac ccgcgtcacc agcacgacgt
     7501 tcgccgcaag ccgcgattgc gccccgaccc ggcaatcccc gccgatcacg caatggggtc
     7561 cgagcacggc gccggcttcg accgcggcgc cggcttcgac gacgcaatgg gggccgacgg
     7621 tcgcgccgga atcgaccgcg gcgccgtccg cgatcaccgc ggtcgggtgg atgccgggca
     7681 cgtccgcaga cgcgcgttcg aacagcgagg cgattcgcgc gtaggcgagg tagggatcac
     7741 gtgcgatgag cgcaggcacc gggctcgcgt cggcatgctc ggcggagagc accactacgc
     7801 cggccccggt gcctgcgagg tcggacgcgt agcggccatt ggcgaggaag gcgagcttgt
     7861 cgccggcggc gcgggcgagt ggcgccacgc cccgcaccac gcccgtgccg tcgccgcgca
     7921 attcgaggcc gaagcgctgc gcgagctcgt cgatgcgata gccggttggt cgttccattc
     7981 gccctggtgc gcttgcggtc gcgtgaattg tatcggcgct cgcgctgttc agcccccttc
     8041 gagtcaaggg ggctgcaaac cgctgccgtg caacggttcg cggggctggg aagcacgcgc
     8101 tttcgttggc gcggcgcgaa cgtgctgcgc tagaacgtgc ggccgaagta gaactgcagg
     8161 cgttcttcga agggcttgtc ttcgggcttg tcgcggatcg gctcggcgag gttgatcacg
     8221 atcgggccga ccggggcgat ccactgcagc gagatgccca ccgaggcgcg caggtccgat
     8281 gcgtcgaacg cggcgtagct cgaatagacg ttgcccatgt cggcgaacag tgaaatccgt
     8341 gccttgtcgt tgtccttgaa gaacggcagc accagttccg cgctgccctt caccttgaac
     8401 gcgccgccca ccggctgcgg gcggtagaag gtgccgccga ggcagttgcc attggacgaa
     8461 atggcagtcg gatccgcctt gccgtcgggg ccgatcaggc ccgagcagat gcgcggcccc
     8521 agggtgttgt cctcgaaccc gcgcacgtcc tggacgccgc cggcgtagaa gttctcgaag
     8581 aacgggaacg acatggtacg gatccggccg gtgctgtccg cgtactgcac cgagatgtcg
     8641 ttggagccgt agaccttgcc gtagccgaga ttgcccgaca cgtgtccgac gaaaccgaac
     8701 ggcagcggga agtagtcgct ggtggcggcg gtgagcttgt agtacggcac ggtcgcgccc
     8761 ggcagcgcga tttcggcgct gatctgctgc aggccgccgc gggtcggctt gaagaacgcg
     8821 ttgagggtgt cgtgcgagta cgacaacgtc aacggcgtgt tgtgcatggt gtagtggccg
     8881 accttgttga tctggtcgaa gaacacctgc ggggtgaacc cggggatcag gtccagcttg
     8941 gtcttgttga tgccgatgcc ggcgttgatg ctgtcggtct cgctgatcgg gaagctgaga
     9001 taggtggaga acgagtcggt gtcggagagg tacgaggcga tgttctgctc tgcctggttc
     9061 aacttcagca cgctggcgtt gtagccgatg ccgatgccgc tgtcggtcag gtacggatcg
     9121 aagaacgaaa cctggtactg cttcaggaac aggctcttcg agaaggtcgc cgaaatgcgg
     9181 ttgccggtgc cgaggaagtt gttgttggcg atcgaggtcg agaggatgat cccggacacc
     9241 tgcgagtagc ccacgccgaa ctggaactgg cccgaggatt cctcggtgac cgacacgttc
     9301 aggtccactt cgtcggccgt gccggggacc ttgacggtgt ccaccgtgac gtccttgaaa
     9361 tagccgaggc gttgcaggcg caccttggaa cggtcgatcg ccgcctgcga ataccaggcg
     9421 ccctcgtact ggcgcatctc gcgtcgcagg atctgcccct gggtctgggt gttgcccttg
     9481 aagttgatcc ggcgcacgta cacgcgcgga cccggcgtga tgtagaaggt gaggtcgacg
     9541 gtatggctgc tctcgtcgac cttcgggatc ggctggacct tggcgaacgc gtagccgatg
     9601 ttggacagca cgtcggtgat cgcgtcggac gacagctcga tctcgtggcg gttgaagatc
     9661 tcgcccggct ggatgcgcag cacctggcgc agcgcgtcct cggg
//
LOCUS       DASSNH010000046         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1468766, whole genome shotgun sequence.
ACCESSION   DASSNH010000046 DASSNH010000000
VERSION     DASSNH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468766"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFK00_01710"
     CDS             complement(<1..653)
                     /locus_tag="VFK00_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7591705.1"
                     /translation="MNRPEELRDKGAQRARFARRALAGFAIMLALLLVVAGRFVYLQV
                     FEHADYSTRARDNRVRLRHLPPPRGLIYDREGRLLADNVPAFRLDVLPDKVGDMDALL
                     HRLSAVVPLTDDDIRRFKQSLKQHRSFEQVPLKFHLSESERDRFAVNQWQFPGVSVAP
                     YLTRNYPYGDEFAHVIGYVGRIDEKELKRIDPDRYAGTTHIGKTGIEKQYETLLHGTP
                     "
     gene            complement(650..1150)
                     /gene="mreD"
                     /locus_tag="VFK00_01715"
     CDS             complement(650..1150)
                     /gene="mreD"
                     /locus_tag="VFK00_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7591706.1"
                     /translation="MNRMRGQGWTFWIGVALALLLMLVPLPAPIEPLKPYWPALVLVY
                     WALEASDRVSVGLAFGIGLAADLLNGALLGDQALRLAVLVFIVVRFRSRLRFFPMWQQ
                     ALAVWLLLLNNHVLQALIRAFAGDSPMPALSWLAPVVGALAWPFLFLLIDDVNTRLRG
                     KGAKAT"
     gene            complement(1147..2094)
                     /gene="mreC"
                     /locus_tag="VFK00_01720"
     CDS             complement(1147..2094)
                     /gene="mreC"
                     /locus_tag="VFK00_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7591707.1"
                     /translation="MALPQKESTPIFSDATAGTLRLIAYLALAAVLMVLDHRNGWLHR
                     ARYATAAAIVPVYKLAALPADWIHDAGTAFTERRKLIDENRQLRETLMLSEARLNRMH
                     AVAQQNERLKQLLDTQHLLGMHAQLARLIDVQLGPTRDRVMLNVGADEGVHVGQTVVD
                     SHGVMGQVVEILPHASVAMLVIDPDHAVPVMVARTGLRGIAHGTGDPGQLIIPDLPLS
                     SDIKTGDQLVTSGLGGRFPGGFPVGTVTALERDPSGMFLQAILKPAAELERSGEVLLL
                     RDQPEPVGPPAAAPPVGPPTSLAPGNGSGAGSPRPRGAP"
     gene            complement(2325..3371)
                     /locus_tag="VFK00_01725"
     CDS             complement(2325..3371)
                     /locus_tag="VFK00_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7591708.1"
                     /translation="MFKLFRGIFSNDLSIDLGTANTLIYVRDQGIVLNEPSVVAIRQD
                     RGAGGPRSVAAVGNEAKMMLGRTPGNIVTVRPMKDGVIADFTMTEAMLQHFIKQVHKT
                     RFLRPSPRVLVCVPCGSTQVERRAIKESAEGAGARDVFLIEEPMAAAIGAGIPVHEAR
                     GSMVLDIGGGTSEVAVMSLNGIVYSQSVRIGGDRFDEAIINYVRRSNGTLIGEPTAER
                     IKIEVGCAIEQKEVRSMEVSGRNLAEGVPRMFTINSNDALEALHEPLHGIVAAVRSAL
                     EQTPPELCSDVQERGIMLTGGGALLRDLDKLISKETGLYVQVAEDPLTCVARGGGRAL
                     ELIDMHGNDFFASE"
BASE COUNT          559 a         1145 c         1222 g          532 t
ORIGIN      
        1 ggcgtgccgt gcagcagcgt ctcgtactgc ttctcgatgc cggtcttgcc gatgtgggtg
       61 gtgccggcgt agcgatcggg atcgatgcgc ttcagttcct tctcgtcgat gcggccgacg
      121 tagccgatga cgtgcgcgaa ctcgtcgccg tacggatagt tgcgcgtgag gtagggcgcg
      181 acgctcaccc ccgggaattg ccactggttg acggcgaagc ggtcgcgctc gctctccgac
      241 aggtggaact tcaacggcac ctgctcgaac gagcggtgct gcttcaggct ctgcttgaag
      301 cggcggatgt catcgtcggt cagcggcacc acggcggaca gcctgtgcag cagggcgtcc
      361 atgtcgccca ctttgtccgg cagaacgtcc agacggaacg ccggcacatt gtcggccagc
      421 agcctgcctt cgcggtcgta gatcagtccg cgcggcggcg gcaggtggcg cagccgtacc
      481 cggttgtcgc gggcgcgcgt cgagtagtcg gcgtgctcga acacctgcag gtacacgaac
      541 cggccggcga ccaccagcag cagggccagc atgatcgcga agcccgcgag cgcgcgccgc
      601 gcgaagcgcg cacgctgcgc gcccttgtcg cggagttcct cgggccggtt catgtcgcct
      661 tcgcgccctt gccgcgcagg cgggtgttga cgtcgtcgat caagaggaac aggaacggcc
      721 aggcgagcgc gccgaccacc ggcgcgagcc aggacaacgc cggcatcggg gagtcacccg
      781 cgaacgcccg gatcagcgcc tgcagcacgt ggttgttgag cagcagcagc cagaccgcca
      841 gcgcctgctg ccacatcgga aaaaaacgca ggcgcgatcg gaagcgcacc acgatgaaga
      901 ccagcaccgc gagacgcagg gcctggtcgc caagcagcgc gccgttgagg aggtcggccg
      961 cgagcccgat gccgaaggcg agcccgacgc tcacacggtc ggatgcctcg agcgcccagt
     1021 acaccagcac cagggccggc cagtagggct tcagcggctc gatcggcgcc ggcaacggca
     1081 ccaacatcag cagcagcgcc agcgcgaccc cgatccagaa ggtccaacct tgtccgcgca
     1141 tgcggttcat ggcgcgcctc gcgggcgcgg cgatccggcc ccggaaccgt tgccgggcgc
     1201 gagcgaagtc ggcgggccca ccggtggcgc ggctgccggc ggacccacgg gctccggctg
     1261 gtcacgcaac aacaggactt cgccgctgcg ctcgagctcg gccgccggct tcaggatcgc
     1321 ctgcaggaac atgcccgaag ggtcgcgttc gagagcggtc acggtgccga ccgggaatcc
     1381 gccgggaaag cggccgccga ggcccgacgt gaccagttgg tcgcccgtct tgatgtcgga
     1441 ggaaagcggc aagtccggga tgatcaactg acccgggtcg ccggtgccgt gcgcgatgcc
     1501 gcgcagcccc gtgcgggcga ccatcaccgg gaccgcatgg tcgggatcga tcacgagcat
     1561 cgcgaccgag gcgtgcggca ggatctcgac gacctggccc atcacgccgt gcgaatcgac
     1621 caccgtctgg cccacgtgca cgccctcgtc ggcaccgacg ttcagcatca cccggtcgcg
     1681 ggtgggcccg agttgcacgt cgatcagccg cgccagttgc gcgtgcatgc cgagcaggtg
     1741 ctgggtatcc aggagctgct tcagcctctc gttttgctgc gcgaccgcgt gcatgcggtt
     1801 gagccgggcc tcggagagca tcagcgtttc gcgcaattgc cggttctcgt cgatcagctt
     1861 gcggcgctcg gtgaaggcgg tgccggcgtc gtggatccag tcggcgggca gggcggcgag
     1921 cttgtagacc ggcacgatcg cggccgccgt ggcatagcgg gcgcgatgca gccagccgtt
     1981 gcggtggtcg agcaccatca gcacggcggc cagcgcgagg taggcgatca ggcgcagggt
     2041 acccgcggtc gcatccgaga agatcggagt ggattccttt tgcggaagcg ccatggcgaa
     2101 gcgggagaga aaggctggaa cgaagtgtgg cgagcgcggg ttggcgcggt ccggcattgt
     2161 gtcacgcttt gcccggagcc cggatgcgtc gctgagcttc ccccttcgga acgaaggcgg
     2221 agcgaagcga acgagcggag cgcgagaggg ggatggcttg ccgcgagacg acatcccccg
     2281 gcgctggcgt gccgcccccc gtccttcgga ggcggtgagg ctttttattc gctcgcgaag
     2341 aagtcgttgc cgtgcatgtc gatgagttcg agcgcccggc cgccgccgcg agcgacgcag
     2401 gtcagcggat cctcggcgac ctggacgtac agcccggttt ccttggagat cagcttgtcg
     2461 aggtcgcgca gcagcgcccc gccgccggtg agcatgatgc cgcgctcctg gacgtccgag
     2521 cacagctccg gcggcgtctg ctcgagcgcc gagcgcactg ccgcgacgat gccgtgcagc
     2581 ggctcgtgca gcgcttccag cgcgtcgttg gagttgatcg tgaacatccg cggcacgccc
     2641 tcggcaaggt tgcgcccgga cacttccatg ctgcggactt ccttctgctc gatcgcgcag
     2701 ccgacctcga tcttgatccg ctcggccgtc ggctcgccga tcagggtgcc gttgctgcgg
     2761 cgcacgtaat tgatgatggc ttcgtcgaag cggtcgccgc cgatccggac cgactgcgag
     2821 tacacgatgc cgttgagcga catcaccgcg acttccgagg tgccgccgcc gatgtcgagc
     2881 accatcgagc cgcgcgcctc gtgaacgggg atgccggcgc cgatcgcggc ggccatcggc
     2941 tcctcgatca ggaacacgtc gcgggcgccg gcgccctcgg ccgattcctt gatcgcgcgg
     3001 cgctcgacct gggtcgatcc gcagggcacg cacaccagca cgcgcgggct cggacgcagg
     3061 aaacgggtct tgtggacctg cttgatgaag tgctgcagca tcgcctcggt catcgtgaag
     3121 tcggcgatca cgccatcctt catcggccgc accgtcacga tgttgcccgg ggtcctgccg
     3181 agcatcatct tggcttcgtt gccgacggcg gccaccgagc gtgggccgcc ggcgccacgg
     3241 tcctggcgga tcgcgaccac cgagggttcg ttgagcacga tgccctggtc gcgcacgtag
     3301 atcagcgtgt tcgcggtacc gaggtcgatc gaaaggtcgt tggagaagat gccgcggaac
     3361 agtttgaaca tcgcgagccc gagacgtatg gcgggtaggc agaacgcgcc agtctagtca
     3421 gcagggggat gccgcgcaag gacggaatcc tcaaaatc
//
LOCUS       DASSNH010000047         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1832592, whole genome shotgun sequence.
ACCESSION   DASSNH010000047 DASSNH010000000
VERSION     DASSNH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832592"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFK00_01730"
     CDS             complement(<1..1083)
                     /locus_tag="VFK00_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7591709.1"
                     /translation="MPNILTSVFGSRNERVLKQLSKIVVRINALESEMQGLADEALRG
                     KTAEFRQRIANGESLDKILPEAFAVVREASRRVIGMRHYDVQLIGGMVLHQGKIAEMR
                     TGEGKTLVATLPVYLNALEGKGVHVVTVNDYLAKRDAAWMGKVYNFLDMTVGVVWPGM
                     EMSEKAEAYGADITYGTNNEFGFDYLRDNMAVAKEQRYQRGLHYAIVDEVDSILIDEA
                     RTPLIISGPAEESPELYVRVNRIVPHLTRQEKEPLPGEPPEPGDYYVDEKQKQVHLSE
                     EGMEHAEQLLRQNGILDEDSSLYDARNLAVVHHLNQGLRAHALYQRDVDYIVRDGEII
                     IVDEFTGRTLPGRRWSEGLHQAVEAKE"
     gene            complement(1327..2253)
                     /locus_tag="VFK00_01735"
     CDS             complement(1327..2253)
                     /locus_tag="VFK00_01735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7591710.1"
                     /translation="MILVARSQRSEWSGTLLEDRRRANRRLCRRLAGGITACVLASAA
                     LAVWIASPSSRTLAQIDSMHRQIASQKAELKQVRENAQRDLSRMAMRLGELQADSARL
                     DALGQRLVVAGKLDPAEFDFSEKPGIGGPETSVPPERSLPYDLSVSMERLGQRFDVQQ
                     TQLGVLQSLLTDRQVTADLIPTGMPVRNGYITSSYGERIDPFTGHESFHPGLDIGVSF
                     GSQVHAVADGLVTYAGVRPGYGEVVEIDHGDGYMTRYAHNSKLLAHVGEHVHAGEVIS
                     DAGSTGRSTGPHVHFEVWHDGKLVNPIAYVRR"
     gene            2287..2751
                     /locus_tag="VFK00_01740"
     CDS             2287..2751
                     /locus_tag="VFK00_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HET7591711.1"
                     /translation="MSPAKPLPPRDTRGPTPVSQCGPVADLAARARVVDLLSQRIVPC
                     LPTPLRDHVGFAGLRNDRLLLLVESPAWATRARMDKARILAAVHSLGFAASSVAAKVA
                     PCLAPSSDSAAPRPLSSRGAQTIRAAASAITDPELRACFLELAAGAENPAAG"
     gene            2959..3129
                     /locus_tag="VFK00_01745"
     CDS             2959..3129
                     /locus_tag="VFK00_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591712.1"
                     /translation="MTEIARIAAAGLGEAAFTDDSGLSRKVWMPVLNSHARNRPHSVD
                     FSNEAAIRDDRT"
     gene            complement(3126..4007)
                     /gene="lpxC"
                     /locus_tag="VFK00_01750"
     CDS             complement(3126..4007)
                     /gene="lpxC"
                     /locus_tag="VFK00_01750"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET7591713.1"
                     /translation="MRATGVGLHTGEKVYMTLRPAAVNTGIVFRRTDLPEPVCIPSRC
                     EYVGDTRLSSTLIRDDVRVGTVEHLLSAMAGLGIDNAYVDLSAPEVPIMDGSAGPFVF
                     LLQSAGIEEQAAPKRFIRIKKPVKVEDGDKWAQFQPFDGFKVGFSIEFQHPIFSRRAS
                     KAEIDFSTTSFVKEVSRARTFGFMRDIEMLREKNLALGGSMDNAIVLDDYRVLNEDGL
                     RYEDEFVKHKILDAIGDLYLLGHSLIGAFFGHKSGHQLNNQLLRTLLADKSAWEEVTF
                     DDPALAPISYAHPAQAV"
BASE COUNT          658 a         1322 c         1295 g          733 t
ORIGIN      
        1 ttccttggcc tcgacggcct ggtggagccc ttcggaccaa cgccggccgg gcaatgtgcg
       61 gccggtgaat tcgtcgacga tgatgatctc gccgtcgcgc acgatgtaat cgacgtcgcg
      121 ctggtacagc gcgtgcgcgc gcaagccctg gttgaggtgg tgcacgactg cgaggttgcg
      181 cgcgtcgtag aggctggaat cctcgtcgag gatgccgttc tggcgcagca gttgctcggc
      241 gtgctccatg ccttcctcgg acaggtgcac ctgcttctgc ttctcgtcga cgtagtagtc
      301 gccgggctcg ggcggctcgc cgggcagcgg ctccttttcc tgacgcgtga ggtgcgggac
      361 gatgcggttg acccgcacgt acagctccgg cgattcctcg gcggggccgg aaatgatcag
      421 tggcgtgcgc gcctcgtcga taaggatcga atcgacttcg tccacgatcg cgtagtgcag
      481 gccgcgctgg tagcgctgtt ccttggcgac cgccatgttg tcgcgcaggt aatcgaagcc
      541 gaattcgttg ttggtgccgt aggtgatgtc ggcgccgtag gcctcggcct tctcggacat
      601 ttccatgccg ggccacacga ccccgaccgt catgtcgagg aagttgtaga ccttgcccat
      661 ccaggcggca tcgcgcttgg cgaggtagtc gttgacggtg acgacgtgca cgcccttgcc
      721 ttcgagcgcg ttcaggtaca ccggcaaggt cgcgaccagc gtcttgccct cgcccgtccg
      781 catctcggcg atcttgccct ggtgcagcac catgccgccg atcaactgca cgtcgtaatg
      841 gcgcatgccg atcacccgcc ggctcgcctc gcgcaccacc gcgaatgctt ccgggaggat
      901 cttgtcgagg ctctcgccat tggcgatgcg ctgcctgaat tcggcggtct tgccgcgcag
      961 ggcctcatcg gccaagcctt gcatctcgga ttcgagcgcg ttgatgcgca ccacgatttt
     1021 cgaaagctgc ttcagcacgc gttcgttgcg gctgccgaag acactcgtca ggatatttgg
     1081 catcggactg ggttcgtatt ccggacctgt ggcgacaggt caatcgatca tgataaaagc
     1141 acacgccgcc cgcgcctagg gatggcggca aaatcggcgg atttcaagaa gcggggtcag
     1201 caagtcccgc gcggagcggc tgttgtgaaa agtcgggcca aggatggccg ttctggactt
     1261 cccgaagttc gaagaccgca accagcgccc cgacacaacg aagcagcgat cagggacgat
     1321 cgcacattat ctgcgcacgt aggcgatcgg attcaccagc ttgccgtcgt gccacacctc
     1381 gaagtgtacg tgcgggccag tcgaacgacc ggtcgagccg gcatccgaga tcacctcgcc
     1441 cgcgtgcacg tgctcgccga catgcgcgag cagcttgctg ttgtgggcgt agcgggtcat
     1501 gtagccgtcg ccatggtcga tctcgacgac ttcgccgtag ccgggacgga cgcccgcgta
     1561 ggtgacgagc ccgtcggcca ccgcgtgcac ctgcgatccg aaggacacgc cgatgtcgag
     1621 gcccggatga aagctctcgt ggccggtgaa cggatcgatg cgctcgccgt acgaggaggt
     1681 gatgtagccg ttcctgaccg gcattccggt cgggataagg tcggcggtga cctggcggtc
     1741 ggtgagcagt gactgcagca cgccgagctg ggtctgctgg acatcgaaac gctggcccag
     1801 ccgttccatg ctcaccgaaa ggtcgtaggg cagtgaacgc tccggaggca cgctggtttc
     1861 cgggccgccg atgccgggct tctcgctgaa atcgaattcc gcggggtcga gcttgcccgc
     1921 gaccaccagg cgctgcccca aggcatccag ccgggccgaa tccgcctgca actcgccgag
     1981 gcgcatcgcc atccggctga ggtcccgctg cgcgttctcg cgcacctgtt tcagttccgc
     2041 cttctgcgac gcgatctgcc ggtgcatcga atcgatctgc gccagcgtgc ggctgctggg
     2101 gctggcgatc cataccgcga gcgccgcgct cgcgaggacg caagccgtga tgccccccgc
     2161 caagcgccgg cacagcctgc ggttggcgcg cctgcgatcc tccagcaagg ttccggacca
     2221 ctcggatcgc tgcgagcggg ccaccagtat catgcccatg tcccgccaac tgccatattc
     2281 atacccgtgt ctcctgccaa gccgctgccc ccccgtgaca cccgcggccc gacgcctgtt
     2341 tcgcaatgtg gcccggtcgc cgacctggcg gcccgggcac gcgttgttga cctcttgagc
     2401 caacggattg ttccgtgcct gccgacgccg ctccgcgatc acgtcggttt tgccggcctg
     2461 cgcaacgacc ggctgctgct gctggtcgag tcccctgcct gggccacgcg cgcgcgcatg
     2521 gacaaggcgc gcatccttgc cgccgtgcat tccctcggtt ttgccgcgag ctccgtcgcg
     2581 gccaaggtcg cgccttgcct ggcgccctcc agtgactccg ccgctccgcg gccgttgtcg
     2641 tcgcgcggcg cgcagaccat tcgcgccgcc gcatcagcca tcaccgaccc ggaattgcgc
     2701 gcgtgcttcc tcgaactcgc cgccggggcg gaaaatccgg cggccggata gcgcgcaagc
     2761 tccgttccgc cgcgtcacct gccgacggaa gccggtttta tagcatgggc ggcgtcgcga
     2821 gtgctgatcg aaaccagcac aatgtcacaa attgtgacac gcgaacagcg ggagaggggc
     2881 gtcccagaag ccgtcgattg gcatggtcga agcggctttg gcgaaaggtc aggccatgga
     2941 aggccgttct ggacccgcat gaccgaaatc gcccgtatcg ccgccgccgg cctcggcgaa
     3001 gccgcattca cggatgatag cgggctttct cggaaagtct ggatgcccgt tctgaattca
     3061 cacgcccgaa atcgccctca ctccgtcgat ttctccaacg aagccgcgat cagggacgat
     3121 cgaacctaaa ccgcctgggc gggatgggcg taggagatcg gcgccagcgc cgggtcgtcg
     3181 aaggtgacct cttcccacgc cgacttgtcg gccaggagcg tccgcaggag ctggttgttc
     3241 aactggtggc ccgacttgtg gccgaagaac gcgccgatca ggctgtggcc gaggaggtac
     3301 aggtcgccga tcgcgtcgag gatcttgtgc ttgacgaact cgtcctcgta gcgcaggccg
     3361 tcttcgttga gcacgcggta atcgtcgagc acgatggcat tgtccatcga cccgcccagc
     3421 gcgaggttct tctcccgcag catctcgatg tcgcgcatga aaccgaaggt gcgcgcgcga
     3481 ctgacttcct tgacgaacga ggtcgtggag aaatcgatct cggccttgga agcgcggcgc
     3541 gaaaagatcg ggtgctggaa ttcgatggag aagccgacct tgaatccgtc gaagggctgg
     3601 aactgcgccc acttgtcgcc gtcctccacc ttcaccggct tcttgatccg gatgaagcgc
     3661 ttgggcgccg cctgttcctc gatcccggcc gactgcagca ggaacacgaa gggtcccgcg
     3721 ctgccgtcca tgatcggcac ctcgggcgcc gacaggtcga cgtaggcgtt atcgataccg
     3781 agccccgcca tcgccgacag caagtgctcc acggtaccga cgcgcacgtc gtccctgatg
     3841 agcgtgctgg acagccgggt gtcgccgacg tactcgcagc gcgaaggaat gcagaccggt
     3901 tcgggaaggt cggtgcggcg gaacacgatg ccggtgttga ccgccgccgg acgcagggtc
     3961 atgtacacct tctcgcccgt gtgcagaccg acgcccgtcg cgcggatc
//
LOCUS       DASSNH010000048         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1465689, whole genome shotgun sequence.
ACCESSION   DASSNH010000048 DASSNH010000000
VERSION     DASSNH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465689"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2262
                     /locus_tag="VFK00_01755"
     CDS             <1..2262
                     /locus_tag="VFK00_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7591714.1"
                     /translation="QKARSLDLATSVGASFPSVAFAGGHSQLVSVAAVDRHWPLRGTE
                     TLAGATGQQYVGHGPAPGNVYLDHRALVALGLKVGDPVQLGGQTLTVAAELLTEPDGG
                     ALFALAPRAQMSLADARAAGLLGVGSRARHGIMVAGGAPAVRQWQAWAKANLPQGAEL
                     ISPEKTQERVRSAFDRAGAFLRLTALLAALLAGVAIALAAHQYARRKIDEVALLRALG
                     TSRRRVLLLLALTLCLLALPGAVFGALLALGIAQAAWSAMHNLIGDVQVALPLAPAGA
                     SALVGLAVLAGFALPPLARLAEVPPVAVFRRDLGRRLRRFDVLYLVPAAVAVALIWLQ
                     GGSGKLAIVVSVSLAAAAAIALGLAALLLWLVRRLAPVAHPALRLGLAALARRRSLTL
                     VQATALGLGLTALLLLGIVAPALLDGWRRELPTDTPNWFVLNLESSQRQGFEHALAGI
                     GADNLNMLPLAVGKLTAINGQPIDKMHFGDPEASRWVDRQLRLSWSATLPPSNRVVAG
                     EWRNQHPATPQVSVDEHWRDMFHLKLGDHMRFQVGEKQIDATVSSIRGVNWRSFRVNF
                     FLLLDPDHAGNLPHTWVASFHLPQAAGDRLATVSREYPNLSMVDIDQLLARIRGIIEQ
                     VAGAVRWVLGFSLLAGALVLAAALSASAAERRHEAALLRTLGAHRRQLRFTAACEFVL
                     LGLISATSAVAAAAGAGAWLARSVFHIEHFVPPVWPLVATGVVATGAVMLLGLAGTWR
                     VAITPPLRLLRRG"
     gene            complement(2881..2957)
                     /locus_tag="VFK00_01760"
     tRNA            complement(2881..2957)
                     /locus_tag="VFK00_01760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2921..2923),aa:Arg,seq:acg)
     gene            complement(3019..3111)
                     /locus_tag="VFK00_01765"
     tRNA            complement(3019..3111)
                     /locus_tag="VFK00_01765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3075..3077),aa:Ser,seq:gct)
BASE COUNT          534 a         1212 c         1113 g          607 t
ORIGIN      
        1 cagaaggccc gcagcctcga ccttgccacc agcgtcggtg cgagctttcc gagcgtcgcc
       61 ttcgcaggcg gccacagcca actggtgagt gttgccgcgg tcgatcggca ctggccgctg
      121 cgcgggacgg aaacgctcgc cggcgccacc ggacaacagt atgtcggcca cggccccgcg
      181 ccgggcaacg tgtacctcga tcatcgtgcg ctggtggcgc tggggttgaa ggtgggcgac
      241 ccggtgcaac tgggcggcca gacactgacc gtcgcggccg aactgctcac cgaaccggac
      301 ggcggcgccc tgtttgcgct ggcgccgcgc gcgcagatga gccttgccga cgcgcgtgcc
      361 gccggtctgc tcggcgttgg cagccgggcg cgacacggga tcatggtggc cggcggggcg
      421 ccggcagtgc ggcaatggca ggcttgggcc aaggcgaacc tgccgcaggg tgcggaactg
      481 atctcgcccg aaaaaaccca ggagcgcgtg cgatccgcgt tcgatcgcgc cggtgcgttc
      541 ctgcgcctga cggcgctgct cgcggcgctg ctcgccggcg tcgcgatcgc ccttgccgcg
      601 caccagtacg cccgccgcaa gatcgacgaa gtggcgctgc tgcgtgcgct cgggacgtcg
      661 cgtcggcgcg tgctcctgct gctggcgctg acgttgtgcc tgctcgcgct gcccggggcg
      721 gtgttcggcg cgctgctggc gctcggcatc gcccaggcgg cctggagcgc catgcacaac
      781 ctgatcggcg atgtgcaggt cgcactgccg ctcgctcccg cgggtgcgtc ggcgctggtg
      841 ggcctcgcgg tattggctgg attcgcattg ccgccgctgg cgcgcctcgc cgaagtgccg
      901 ccagtcgcgg tgttccgccg cgacctcggc cgacggctgc gccgcttcga cgtgctgtac
      961 ctcgtgcctg cggcggtggc ggtagccttg atctggctgc aaggcggctc ggggaaattg
     1021 gcgatcgtcg tttccgtgag cctcgccgcg gccgcggcaa ttgcgttggg attggccgcg
     1081 cttctgctct ggctggtgcg gcgcctcgcg ccggttgcac atccggcgct gcggctcggc
     1141 ctggcggcgt tggcgcgaag acgcagcctc acactcgtgc aggcgaccgc actcggcctc
     1201 ggcctcaccg cgctgctgct cctgggcatc gtcgcacccg cgctgcttga cggatggcgc
     1261 cgcgagctgc cgaccgatac ccccaactgg ttcgtgctga acctcgagag cagccagcgc
     1321 cagggattcg agcacgcgct agccggcatc ggcgccgaca acctcaacat gttgccgctg
     1381 gccgtgggca agctgaccgc catcaacggc cagccgatcg acaagatgca cttcggcgac
     1441 ccggaagcat cgcgctgggt ggatcggcaa ctcaggcttt cgtggagcgc gacgctgccg
     1501 cccagcaacc gcgtggtcgc gggagagtgg cgcaaccaac atcccgcgac accccaggtt
     1561 tcggtcgacg agcattggcg cgacatgttc cacctcaagc tcggcgacca catgcgcttc
     1621 caggtcggcg aaaagcagat cgacgccacc gtatccagca tccgcggggt caactggcgc
     1681 tcgttccgcg tcaacttctt cctgctgctc gaccccgatc acgctggcaa cctgccgcac
     1741 acctgggtcg cgagcttcca tctgccgcag gctgccggcg atcgcctggc gacggtatcg
     1801 cgcgagtacc ccaacctgag catggtcgac atagatcagt tgctcgcacg catccgcggc
     1861 atcatcgaac aggtcgccgg cgcggtgcga tgggtgctcg gcttcagtct cctggcaggc
     1921 gcgctggtgc tggccgcggc gctctccgcg agcgccgccg agcgccgcca cgaggccgca
     1981 ctgttgcgaa cgctcggcgc gcatcggcgg cagttgcgat ttaccgcggc gtgcgaattc
     2041 gtgctgctcg ggctgatctc cgccacgagc gccgtcgccg ccgctgccgg cgccggcgcg
     2101 tggctcgcgc gcagcgtgtt ccatatcgag cacttcgtgc caccggtctg gccactcgtc
     2161 gctacggggg tcgtggcaac gggcgccgtg atgttgcttg gactcgcggg cacttggcgg
     2221 gtggcaatca cgccgccgct gcggttgctg cgccgcgggt gagccgcgtc ggagacggca
     2281 ccgctaccgc cccaaatgac accagcgaga tcgctggtgt cattgcgctt cgaacggaag
     2341 cctgccgctg cggcgatccc ccgctgtgcg ccttttccct agaacggctc ccaacccaac
     2401 gcaaccaagt gttcggcaat ggaaagcggc aaccgtaccg cccggcgcga gtttccccgc
     2461 accttgcccc catctgaatc gcagtgtccc cctgccactt cacacccccc ttttcacaca
     2521 cctgttcctg acaacacgca cccataaacc agttggtacg agtcgtatag catcatgatc
     2581 tttcgacgac ctttcgcaca ggtggagagg ggaggtcgtg cacgacgacg gcatggctcg
     2641 acctgcttca cttgcgcact gacacctgct cggtccgcgc gttggaacga gatccgtccg
     2701 gaatgctgcg ggttgtttgg ggatacacaa agcacgccga atcggccaac tcccaaaatg
     2761 tctccccatc gcacctccgc ttgccagcgc cgccctgttc ccgccggaaa caaaaaaccc
     2821 cgcacgaatg cggggtttca gggtttcatg cgacgttgtg atccgtgctg aaacagctat
     2881 tggcgcgccc ggagagattc gaactcccga ccaccaagtt cgtagcctgg tactctatcc
     2941 agctgagcta cgggcgcatg gaaacgctga agcgagccgc gaattatccg gtgttgcgat
     3001 aatcgcgtca acggaaactg gcggagagag agggattcga accctcgatc aggcttttga
     3061 cccgatactc ccttagcagg ggagcccctt cggcctctcg ggcatctctc cgaattttga
     3121 aaaatctttc aaaccaacca tccccggggc gcctcccagt cggcatccat gcctcctcct
     3181 caccgctcgc acgcgatgcc accggctcac gcaagcgctc gcgtctcgat ctctcgggca
     3241 tccctccgaa gtctgcggcg gcgttcggat gtggattcca ctcgcgcacg caacgatcga
     3301 ccggcaaaac cggtcgatca ggataaaggc atctccactt gcggtaaagc cgcgcgacgc
     3361 tccggcatca gccgtgggtc gcttcgtgcc ggacaggctc gtcggactcg gcagatgcac
     3421 cgccacccgc ctcgtgttcc tgcttgatgc gctggtagat ctcttc
//
LOCUS       DASSNH010000049         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_585957, whole genome shotgun sequence.
ACCESSION   DASSNH010000049 DASSNH010000000
VERSION     DASSNH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585957"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFK00_01770"
     CDS             <1..1173
                     /locus_tag="VFK00_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET7591715.1"
                     /translation="QVLGRRDTPVDRPLPDQLISPPRAGLPPQSAHSDSRLDPRPLPT
                     ARTAIGRGGKVDAGSALRGAAASVPGQGGAVSTRFSTAARIIADILMRFPASPAAIRA
                     QVPLLTTRERVQVTQLAARLRDSIESSGLFYESHLLRWRGGTFPLAQLLREPQVRWSG
                     RAGTPPQASFQPPPQGNAPSAAAPAPSSSATGTPSTHAPVGPAAPVPSVATAVVAPAQ
                     GVDGKPMASPVAGRADAPALAAQTGAVQLAPTRDQPVHPALEGVLRHQLEMLAAPVLR
                     WEGTPWAGAFMALTLQPPARQPEREFPGEERTPRQDGDAPWQTRLTLRLARLGEVRVD
                     LQLGAQRVALDLQAEPDAVGRMQADRDALRERLAALGFRSVTLRIHAPDLPEAGHD"
     gene            1166..1459
                     /locus_tag="VFK00_01775"
     CDS             1166..1459
                     /locus_tag="VFK00_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET7591716.1"
                     /translation="MTEQKRRQAVALAGSDSGEAARVIAKGYGDLAEQVIAEARRHGI
                     YVHDSPELVGLLMGLDLDQRVPDRLYDVVAELLAWVAEMDAGTAAAAMTRSPE"
     gene            1553..>2386
                     /locus_tag="VFK00_01780"
     CDS             1553..>2386
                     /locus_tag="VFK00_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HET7591717.1"
                     /translation="MSEESDDQEKTEPATLRRLEQAREEGQVARSRELTTFLLLGGGL
                     LGLWAMGSRLSRQLGMMMERAFLFDRTQAFDLHVTMSRVFELGSHSLLAIAPLLLGLV
                     VLALISPLLLGGWSMSAKAMAPKLSKLNPVQGFKRMLSAQMLTELGKAIVKSLLIGGM
                     AVWFLMRHRGGLVALMGEPVMQAIPDALGLAISASGLMVLALLVVVGIDVPWQLFSHA
                     KKLRMTKEEIRRENKETEGDPHVKGRIRSQQQAMARGRMMSKVPDADVIVTNPTHYAV
                     AL"
BASE COUNT          367 a          817 c          840 g          362 t
ORIGIN      
        1 caggtattgg gccggcgcga taccccggtg gaccggccgc tgcccgacca gttgatctcg
       61 ccgccgcgcg cgggcctgcc gccgcaatcg gcgcacagcg attcgcggct ggacccgcgt
      121 ccattgccga ccgcccgcac cgcgatcggc cgcggcggca aggtggatgc cgggtccgcg
      181 ctccgcggag ccgcggcctc cgtgccggga cagggcggcg ccgtcagcac ccggttcagc
      241 accgccgccc gcatcatcgc ggacatcctg atgcgtttcc ccgcttcgcc cgcggccatt
      301 cgtgcccagg taccgctgct caccacccgc gagcgcgtgc aggtcacgca attggccgcg
      361 cgcttgcgcg acagcatcga gtccagcgga ttgttctacg aatcgcacct gctgcgctgg
      421 cgcggcggta cgttcccgct ggcgcagttg ctgcgcgagc cgcaggtgcg ctggagtggc
      481 cgcgccggta cgccgccaca ggcgtcgttc caaccgccac cgcaaggcaa cgcaccatcc
      541 gcggccgctc cggcgccatc ttccagcgcc acgggtacgc catccacgca tgcgcccgtc
      601 ggccctgccg cgccggtccc gtcggtcgcg acggccgtcg ttgcacccgc ccaaggcgtc
      661 gacggcaagc cgatggcgag ccctgtagcg gggcgcgccg atgcgccggc cctggccgcg
      721 caaaccggtg cggtacagct ggcgccgacg cgcgatcagc cggtgcatcc cgcattggaa
      781 ggcgtattgc ggcaccagct cgagatgctg gccgcgccgg tgctgcgctg ggaaggcacg
      841 ccgtgggccg gcgcgttcat ggcgctgacg ttgcagccgc cagcgcggca gcccgaacgg
      901 gaattcccgg gcgaagaacg aacgccgcgg caggatggcg atgcgccatg gcaaacccgc
      961 ctgaccttgc gtcttgcgcg gctgggcgag gtgagggtcg acctgcaact cggcgcgcaa
     1021 cgcgtcgcgc tggatctcca ggccgaaccg gatgccgtcg ggcgcatgca ggccgatcgc
     1081 gacgcgctgc gcgaacggct cgccgcgctg ggattccgga gcgtgacgtt gcgcatccac
     1141 gcccccgatc tgccggaggc cggccatgac tgagcagaag cgccgccagg ccgtggcact
     1201 ggccggttcc gacagcggcg aggccgcgcg cgtgatcgcg aaaggctacg gcgatctcgc
     1261 cgagcaggtg atcgccgaag cacgccgcca cggcatctac gtgcacgact cgcccgaact
     1321 ggtcggcttg ctgatgggcc tcgatctcga ccagcgcgtg cccgaccggc tgtacgacgt
     1381 ggtcgcggaa ttgctggctt gggtggcgga aatggatgcc ggcaccgcgg ctgctgcaat
     1441 gacccggtcc cccgaatagc cgccccgatt ccgcccttat cgcgggatgg gcgaggggcc
     1501 cggcgcccca caatggcagt cgagaaactg ccgttgccgc gccgcaccca gcatgtccga
     1561 agaatccgac gatcaggaaa agaccgaacc ggccaccctt cgccgcctcg aacaggcgcg
     1621 cgaggaaggg caggttgcgc gctcgcgcga attgacgact ttcctgttgc tgggcggtgg
     1681 gctgctggga ctgtgggcga tgggctccag gctgtcccgg cagctcggga tgatgatgga
     1741 gcgggcgttc ctgttcgacc gcacgcaagc cttcgacctg cacgtgacga tgagccgcgt
     1801 gttcgagctc ggctcgcaca gcctgctggc gatcgcgcca ttgctgctcg ggctggtggt
     1861 gctcgcattg atttcgccgc tgctgctggg cggctggtcg atgtcggcca aggcgatggc
     1921 gccgaagctg tccaagctca atccggtcca gggtttcaag cggatgcttt ccgcgcagat
     1981 gctgaccgaa ctcggcaagg cgatcgtgaa gtcgctgctg atcggcggca tggcggtctg
     2041 gttcctgatg cgccatcgcg gtggcctggt tgcgctgatg ggcgagccgg tgatgcaggc
     2101 catcccggat gcactcggcc tggcgatcag cgccagcgga ttgatggtgc tggcgttgct
     2161 ggtggtggtc ggaatcgacg taccgtggca gttgttcagc cacgccaaga agctgcggat
     2221 gaccaaggaa gaaatccgtc gcgaaaacaa ggaaaccgaa ggcgatccgc acgtgaaggg
     2281 ccgcatccgc tcgcagcagc aggcaatggc gcgcggacgc atgatgagca aggtgccgga
     2341 tgcggacgtg atcgtcacca atcccaccca ctacgcggtg gcgctg
//
LOCUS       DASSNH010000050         4913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_459166, whole genome shotgun sequence.
ACCESSION   DASSNH010000050 DASSNH010000000
VERSION     DASSNH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459166"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /gene="rimO"
                     /locus_tag="VFK00_01785"
     CDS             complement(<1..140)
                     /gene="rimO"
                     /locus_tag="VFK00_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037694.1"
                     /note="catalyzes the methylthiolation of an aspartic acid
                     residue of the S12 protein of the 30S ribosomal subunit;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7591718.1"
                     /translation="MLASSPKVGFVSLGCPKALVDSERILTQLKAEGYEIVPRYDKAN
                     AV"
     gene            264..941
                     /locus_tag="VFK00_01790"
     CDS             264..941
                     /locus_tag="VFK00_01790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7591719.1"
                     /translation="MGQHISLNTAGTHCIGAYLAKPEGNPKGGIVVAQEIFGVTAHIR
                     EVADGFAKEGYTAIAPAFFDYVENDVELDYDKAGTERGKALATEVGLDRAVEAVASAA
                     DSIRSSGRIGVVGYCWGGTVALLAAQRLGLPSVSYYGARNVPFLDQPLKASVMFHFGA
                     KDRSITADAIQKHRDAYPDAPVYVYEDAGHAFNRDVDPGHYDPVGAPLARQRTLAFFD
                     RHLAGKA"
     gene            938..1351
                     /locus_tag="VFK00_01795"
     CDS             938..1351
                     /locus_tag="VFK00_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET7591720.1"
                     /translation="MSRDADFELDPRLAGDTAFVADWPLCRVLLMDDARYPWLLLVPR
                     RRGAVELDDLRESERMQLMHEIQRAMDALRGVAEFDKLNVGALGNIVRQLHVHVLARS
                     KDDDAWPGPVWGHGSARRYPADARDALVTRFRSAS"
     gene            1354..2085
                     /gene="pxpB"
                     /locus_tag="VFK00_01800"
     CDS             1354..2085
                     /gene="pxpB"
                     /locus_tag="VFK00_01800"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HET7591721.1"
                     /translation="MEFAIEALAEDTLLLRFGEAIDLATNARVHAAARALRVAALRGV
                     VDIAPAYATLLVRFDPCTRSHEELAEALRAATAETLDPGLRRDEESKHDSPPGASTGM
                     VAIPVCYGGDHGPDLEAVAKHAELSPDEVTARHTAAEYTVAMIGFAPGFPYLLGLDPA
                     LAMPRRADPRTRVPAGSVAIGGAQTGIYPRELPGGWQLIGRTPLSLFDPARAPPCLLA
                     PGDRVRFRAIGRDEFAKLAGHTSGD"
     gene            2075..3052
                     /locus_tag="VFK00_01805"
     CDS             2075..3052
                     /locus_tag="VFK00_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HET7591722.1"
                     /translation="MAIEVVKPGLLTTLQDLGRAGHAHLGIGRAGAFDVPAMRIANAL
                     CGNPGDACGLEMTLLGPTLRFDAGTWIAVTGAPMAIDLEGTECPMWAPVFVPAGASVT
                     LGAVRAGCRSYLGVGGGVDVEPVLGSRSTDLNAELGPHSGRPLGVGDTLASGTDTGDA
                     EPATPKWRLDPRPWYFEDSSKPLRLLPGNHLDRLTTISRKQLFSDDFTVHTDSNRVGV
                     RLAGPTLECSAPIEMISEGFVPGLLQLPPSGQPIAFGPECPVSGGYPRLGTIAAVDIP
                     RLAQLRPGDALRFRACTMDEASDALRKRERALDELEAAIRHRLSRGDRH"
     gene            3211..3978
                     /locus_tag="VFK00_01810"
     CDS             3211..3978
                     /locus_tag="VFK00_01810"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET7591723.1"
                     /translation="MKSIDLNADLGESFGAWRMGDDAGVMPWITSANIACGFHAGDPL
                     TMRRTIALCIGNGVAIGAHPSLPDLQGFGRREMRIDPGEAYAQTLYQVGALHAFAKAG
                     GGKLHHVKPHGALYNMAARDAGLADAVACAVRDFSPTLILVGLAGSALIEAGRNAGLP
                     VLREAFCDRRYQGDGSLAPRSEAGAVIEDPQAAVAQALSIAMRGEAVALDGTRIRIEA
                     DTLCVHGDRAGAAGFAKVLRRALENAGLNVDAMERPA"
     gene            3975..4730
                     /locus_tag="VFK00_01815"
     CDS             3975..4730
                     /locus_tag="VFK00_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET7591724.1"
                     /translation="MMHWLPLLGVLVIVIGFALRFNPVPVVVAAAIVSGLAAGKSIGD
                     ILALIGNSFVSERALLLFVLTLPAIGVLERAGLREHARNWISNLRGLTLSRLLIAYLG
                     LRQILSMLGLTNVAGQAQTVRPLLAPMAEAAAEQRGQPLDDEERNDVRAFSAGTDNIG
                     LFFGEDVFIALGAVLLIQGFYSQHGIELEPLQIALWALPTAVAAFVIHSIRTVLFARR
                     IRNMTTGSTAFAGPRPDGSPNERQNLLGTDRRE"
BASE COUNT          808 a         1628 c         1665 g          812 t
ORIGIN      
        1 atcaccgcgt tggccttgtc gtagcggggc acgatctcgt agccctcggc cttcaactgg
       61 gtgaggatgc gttcggaatc gaccagcgcc ttggggcagc cgaggctgac gaaaccgact
      121 ttcggggacg aagcaagcat cgcgaatttg cgtcagcgaa aaccggtcat cttagcggga
      181 ctcgcggctc cgcacggccg aaggccgcgt ccgccgtggc cgcgtagtac gctgccggga
      241 tcgcgacttt cggaggaccg cccatgggtc agcacatcag tctcaacacg gccggcacgc
      301 attgcatcgg cgcctacctc gccaagccgg agggcaaccc caagggcggc atcgtggtgg
      361 cgcaggaaat cttcggcgtc accgcgcaca tccgcgaggt cgccgacggc ttcgctaagg
      421 agggctatac cgcgatcgcg ccggcattct tcgactacgt cgagaacgac gtggaactcg
      481 attacgacaa ggcgggcacc gaacgcggca aggcgctcgc cacggaagtc ggcctcgatc
      541 gcgcggtgga agccgtggcg agcgccgccg attcgatccg gtcgtcgggc cggatcgggg
      601 tggtcggcta ctgctggggc ggcacggtcg cgttgctcgc cgcgcaacgc ttggggctgc
      661 cgtcggtgag ctattacggc gcgcgcaacg tgccgttcct cgatcagccg ctgaaggcgt
      721 cggtgatgtt ccatttcggc gcgaaggatc gctcgatcac cgccgacgcc atccagaagc
      781 accgtgacgc ttatccggat gcgccggtgt acgtgtacga ggatgccggc catgccttca
      841 accgggacgt ggatcccggc cactacgacc cggtcggcgc gccgttggca cgtcagcgca
      901 ccctcgcatt cttcgaccgg caccttgcgg gcaaggcatg agtcgggacg ccgatttcga
      961 actcgacccg cgactcgccg gggacacggc gttcgtcgcc gactggccgc tctgtcgcgt
     1021 actgttgatg gacgacgcgc ggtacccatg gctgctcctg gtcccccgcc gccggggcgc
     1081 ggtcgagctg gacgacctcc gggaatccga gcgcatgcaa ttgatgcacg aaatccagcg
     1141 cgccatggat gcgctgcggg gtgttgccga attcgacaag ctcaacgtcg gcgcgctcgg
     1201 caacatcgtg cggcaactgc acgtgcacgt gctcgcacgc agcaaggacg acgacgcttg
     1261 gcccggcccg gtgtggggcc acgggtccgc gcgacgttac cccgcggacg cgcgtgatgc
     1321 cctggtcact cgcttccggt cggcatcgtg accatggaat tcgcgatcga ggcgttggcc
     1381 gaggacacgc tgttgctgcg cttcggcgaa gctatcgacc tcgcgacgaa tgcacgggtg
     1441 catgcagccg cgcgggcatt gcgggtagca gcgttgcggg gcgttgtcga catcgcgccg
     1501 gcctatgcga cgctcctggt gcggttcgat ccatgcaccc gatcccatga ggaattggcc
     1561 gaagccttgc gcgccgcgac cgccgaaaca ctggaccccg gcctgcgccg ggatgaggag
     1621 tcaaaacatg attccccgcc cggcgcgagc accggcatgg tcgccattcc ggtgtgctac
     1681 ggaggcgatc acggtcccga cctcgaagcc gtcgcgaaac acgcggaatt gtcgccggac
     1741 gaggtgaccg cacgccacac ggcagccgaa tacaccgtcg cgatgatcgg cttcgcaccg
     1801 gggttccctt acctgctagg cctcgacccc gcgctcgcga tgccgcggcg tgcggacccg
     1861 cgcacccgcg tcccagccgg ctcggtcgcg atcggcggcg cgcagaccgg catctatccg
     1921 cgcgaattgc cgggcggctg gcaattgatc ggtcgcacgc cgctgagcct gttcgaccct
     1981 gcaagggcgc caccttgcct gctcgcgccg ggcgaccgcg tgcgctttcg tgcgatcggc
     2041 cgtgacgaat tcgcgaaact cgccgggcac acgagtggcg attgaagtcg tcaagccggg
     2101 gctgctgacc accctgcagg acctgggccg ggccggccat gcgcacctgg gcatcggccg
     2161 ggcgggcgcg ttcgatgtgc ccgcgatgcg catcgccaac gcgctgtgcg gcaacccggg
     2221 cgatgcgtgc ggcctggaaa tgacgctgtt gggaccgacg ctgcggttcg acgcgggcac
     2281 atggatcgcg gttacgggcg cgccgatggc gatcgacctc gagggaaccg aatgccccat
     2341 gtgggcgccc gtgttcgttc ccgccggcgc atcggtgacc ttgggcgcgg tccgcgccgg
     2401 ctgtcgcagc tatctgggcg tgggcggcgg cgtcgacgtg gaaccggtgc tgggcagccg
     2461 cagcaccgac ctcaacgcgg aattgggtcc gcactcggga aggccgctgg gcgtgggcga
     2521 cacgctcgcc agcggaaccg acacaggcga tgccgaaccc gcaacaccca aatggcggct
     2581 cgatccgcgc ccctggtatt tcgaagattc gagcaaaccg ctgcgactgt tgccgggaaa
     2641 tcacctcgac cggcttacaa ccatatcgcg caagcaactg ttttcagatg attttacggt
     2701 acataccgat tcaaatcgcg tgggcgtgcg cttggcgggc ccaaccctcg agtgttccgc
     2761 tcccatcgag atgatcagcg aaggattcgt tccgggactg ctgcagttgc cgccctccgg
     2821 acaaccgatc gccttcgggc ccgagtgtcc ggtcagcggc ggctatccgc ggctgggaac
     2881 gatcgcggcg gtggacatcc cgcgactcgc gcaactccgt cccggcgatg cgttacgctt
     2941 ccgcgcatgc acgatggacg aggcatcgga cgcgctgcgc aaacgcgaac gcgccctcga
     3001 tgaactggaa gccgcaatcc gccaccgcct gtctcgagga gacaggcatt gaagcaacga
     3061 ctctggatcc cggatatcgc gccgcgactg cgtttgcaaa attgcaaacg cgaaccgaac
     3121 aggcggcgcg cagcgcgagc gccatcgatg gcgcgagtat tccgggatga cgaataaaat
     3181 cagcgattcc cggcccctac tcctactccc gtgaaatcca tcgacctcaa tgccgacctc
     3241 ggggaatcct tcggcgcctg gcgcatgggc gacgacgccg gggtgatgcc gtggatcacg
     3301 tcggccaata tcgcttgcgg cttccatgcc ggcgatcccc tcacgatgcg gcgaaccatt
     3361 gcgctatgca tcggaaacgg cgtcgccatc ggcgcgcatc cgtcgctgcc ggacctgcaa
     3421 ggtttcggac gccgcgagat gcgaatcgat cccggcgagg cgtacgcgca gacgctgtac
     3481 caggtgggcg ctttgcacgc cttcgccaaa gccgggggcg gaaagctgca ccacgtcaag
     3541 ccgcatggcg ccctctacaa catggccgca cgcgacgccg ggctggcaga tgcggttgcg
     3601 tgcgcggtcc gcgacttcag ccccacgctg atcctggtcg gactcgcggg aagcgcgctc
     3661 atcgaggccg gacggaatgc cggcttgccg gtgctgcgcg aggccttctg cgaccgccgc
     3721 taccagggtg atggctcgtt ggcaccgcgt tcagaggccg gcgcggtgat cgaagatccg
     3781 caggccgccg tagcgcaggc gctgtccatt gcgatgcgcg gcgaggcggt cgccctcgat
     3841 ggcacgcgca tccgcatcga ggccgatacg ctgtgtgtgc atggcgaccg cgccggtgcg
     3901 gccgggttcg cgaaggtgtt gcggcgcgcc ctggaaaacg ccggcctgaa cgtcgatgcc
     3961 atggaacgcc cggcatgatg cactggttgc cgttgctcgg cgtgctggtg atcgtcatcg
     4021 gcttcgcgct gcgcttcaac ccggtaccgg tggtggtcgc agcggcgatc gtcagcgggc
     4081 tcgccgcggg caagtccatc ggcgacatcc tcgcgctgat cggcaacagc ttcgtgtccg
     4141 aacgggcact gctcctgttc gtgctgacgc taccggcgat cggggtgctg gaacgcgcgg
     4201 ggctgcgcga gcacgcgcgc aactggattt cgaatctgcg cggcctgacc ctctcgagat
     4261 tgctgatagc ctacctcggc cttcgccaga tcctgtcgat gctcgggctg accaacgtcg
     4321 ccggccaggc ccagacggta cgtcctttac tggcgccgat ggccgaggcc gccgccgaac
     4381 agcgcggcca gccgctggat gacgaggaac gcaacgacgt cagggcgttc agtgccggga
     4441 ccgacaatat tggattgttc ttcggcgagg acgtgttcat cgcgctgggc gcggtgctgc
     4501 tgatccaggg cttctattcg cagcacggca tcgaactcga gccgctgcag atcgccctgt
     4561 gggcgctgcc gaccgcggtc gcggcgttcg tcatccactc gattcgaacg gtgctgttcg
     4621 cgcgccggat aaggaacatg actactggct ccacggcttt cgcgggtccg cgcccggacg
     4681 gatccccgaa cgaacgacaa aacctgctgg gcacggaccg acgggaatga ccgcggaagg
     4741 agagctcggt acgatgaccc gtggatttca aacagcatgc tgaagatcga gcacgcctac
     4801 tggctgatcg cgctgttcct ggccggggcg gcatggttca acctgcgcga ccggcgctgg
     4861 tggcacgcag cgttctggct ggtgctggcg gcgctgatgg cgggtggccg cta
//
LOCUS       DASSNH010000051         6750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_871945, whole genome shotgun sequence.
ACCESSION   DASSNH010000051 DASSNH010000000
VERSION     DASSNH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871945"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFK00_01820"
     CDS             complement(<1..228)
                     /locus_tag="VFK00_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7591725.1"
                     /translation="MNTNLRRNWLVQGIASVGECGLFFVAILGALPKSLRYWRETCRQ
                     VWFVGAMSLVIVMTCGLFVGMVLALQLYYTLS"
     gene            complement(225..1061)
                     /locus_tag="VFK00_01825"
     CDS             complement(225..1061)
                     /locus_tag="VFK00_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7591726.1"
                     /translation="MPQAHAQSDNSAPMVQVRHLRTRLGGKVIFDGLDLEVPRGSVTA
                     VMGPSGTGKTTLLRHLTGQLRPDAGSVRVDGEEVTTLSRRALFGLRERIGFLFQNSAL
                     LTDFSVFENVAFPLRQHTRLGEELVRNIVLTKLQAVGLRGAANLMPSELSGGMARRVA
                     LARAIVFDPMLILYDEPFVGLDPIALNQVLRLIRTLNQTLGITSILVAHELAAVRQVA
                     DRVYLIAGGKLVAEGAPDELAHRDSPWTRQFFGGEADGPVPFHYPAPDFASQLGLAES
                     PR"
     gene            complement(1130..2821)
                     /locus_tag="VFK00_01830"
     CDS             complement(1130..2821)
                     /locus_tag="VFK00_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7591727.1"
                     /translation="MRFGPVRALGFVVLMVAAIAWIPRAQAGQVSPCKAPAWAPSSMP
                     GFVIASCDSKAWDSTDLDLPAGSKTIAGRRTEVNYALKDQSKDPSAAAARDFHIAAGK
                     RAGATLASDPTDSWKAILTQKTPQGEYWYVYTHGSGNDESTTSYTLTTWKIVPLQQAV
                     IAQPMKASLPAPGGACGDPPWLMEQFAYFKADSCEAKAWDQVKLDLPSGEKTLEGRRL
                     TVNYKLTDENKDPVAFAVQKNYIAALRKIGAKLMSDPTNGFEATLMQKTPAGEFWYLY
                     KHGSGNEESTGAYSLTTVDVAPFPQVVVAQLPQGALLDDMQGKGCKSPPWLVKQFDYY
                     KLTDCSKRDLDSITLDLPSGQKTLAGRFLELNYTLIDEKKDASALYVQKNYVNALEKI
                     GAKLVSDPEDANQAVLTQKVPQGELWYIYEHTGGNEESTGSYSLTALQIGGPPPRACK
                     LEVYGINFDFNKAGIKPESEPVLNQMLALFTADRAYKAEVGGHTDNVGGKAYNMKLSG
                     QRAEAVRAWLVGHGVAADRLTSHGYGFSHPLVPNNSDANRAKNRRVELKRQDCHT"
     gene            complement(2883..3518)
                     /gene="gmk"
                     /locus_tag="VFK00_01835"
     CDS             complement(2883..3518)
                     /gene="gmk"
                     /locus_tag="VFK00_01835"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7591728.1"
                     /translation="MSDRASATGRLFVVAAPSGAGKSTLVNALLAREPGISLSISHTT
                     RAPRPGDVDGVQYHFVDRATFAKMVAEGEFLEHAEVFGNGYGTSRGAVEPVLAAGRDV
                     LLEIDWQGAAQVRRAMPECVSIFILPPSCAELERRLRARASDAEATIARRLAESREEM
                     SHAHEFDCVIVNDVFEDALAQLQAIVHAVRSGEPLPQDGHPGLLAELLGQG"
     gene            complement(3746..5446)
                     /locus_tag="VFK00_01840"
     CDS             complement(3746..5446)
                     /locus_tag="VFK00_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HET7591729.1"
                     /translation="MKSFHSLRRGLFAVTVSLALAGTVQAQQAPTVPSQLPNAGELLQ
                     QVPRQPESAPSSDLDLRMLHVQRRPVHDVHAFLVKRIRITGNTLLPTSTLHALVSDSE
                     GHRLTLGDLQDLADRISEAYHAAGYPLARAYVPAQTLRDGVVKIDVVEARYGKIVLQN
                     HSSVSDKTLRKVLAPLQPGQPVANRPLERSLLLLSDVPGATASSVVRPGDQPGTSDLV
                     VDVTAAPRYTGTIGVDDFGNRYTGRARVNGGFDVNGLLHRGDLLQLGAASSGGGMSYF
                     QGGYRMLLGGQGTTLRAGVSTLHYHLRHGLEALDAHGSALVGSLALAHPFIRSTAGNL
                     YGQIEFDRKLLKDDIEAALLHNDRHFDVGSAMLAGDQRDVHGITNFRIGVSHGKVDFT
                     DSLAEVIDQLTARTAGGYSRYVVSVSRLQQLDPANAIYVGYNRQWASKNLDTSAQFFL
                     GGPDTVRGYDTGALSGSQGDLLTAEFRHDFHLPSMPGRWQATLFADTGRVEVYKDAFV
                     GGVNSGRASSAGVGLHWATPGEWFVDVSASSPIGATPALLDAADAGTRLWVQVRKGFY
                     "
     gene            complement(5505..>6750)
                     /locus_tag="VFK00_01845"
     CDS             complement(5505..>6750)
                     /locus_tag="VFK00_01845"
                     /inference="COORDINATES: protein motif:HMM:NF037374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HET7591730.1"
                     /translation="QDKVYDAGTAGIATLSSSGVLTGDTVDFDYGTATFADANAATGK
                     TVSVSGITKTGTDAGNYTINATATTTADITPFVLNLSGTRVYDSTAGADASLFGDNGV
                     LDGVNGERLTLSGVGTLTDKNVAEQKPFASTAGFTLAGNGTTLASDYTLVGGSDWVTI
                     TPATLAVTGTKATDRTYDGSRVDALSGAVLAGLLGNDDVTLGNAASGLFADANAGTGK
                     AVTTSMTIDGEDSGNYVLQQPMGLVADIAPRPVTVTATGTDKMFDGNTTATVTLAGEG
                     VVSGDEVGFSHEAANFSDSNVDDDKAVTVTGIRLTGADAANYVLVDPVATTTASITGA
                     QLSAFGVDNGVLAYLQSPIRPASVATPYGLAIQHGEGSFTGNRKLLHRPIERHRTRPD
                     FTSGMALKVVDGGVRMPAKGAR"
BASE COUNT         1148 a         2258 c         2173 g         1171 t
ORIGIN      
        1 cgacaacgtg tagtaaagct gcagcgccag caccatgccg acgaaaagcc cgcaggtcat
       61 cacgatcacg aggctcatcg cgccgacgaa ccagacctgc cggcacgtct cgcgccagta
      121 gcgcaggctc ttgggcagtg caccgaggat cgcgacgaag aacaacccgc attcgccgac
      181 cgaggcgatc ccctggacca gccagttgcg gcgcaggttc gtgttcatcg cggcgattcc
      241 gccagaccga gttgggatgc gaagtcgggc gccggataat ggaacggcac cgggccgtcc
      301 gcctcgccgc cgaagaactg ccgcgtccat ggcgagtcgc gatgcgcaag ctcgtccggg
      361 gcaccctcgg ccaccagctt gccgccggcg atgaggtaca cgcggtcggc cacctggcgg
      421 accgccgcga gttcatgcgc gaccaggatc gaggtgattc cgagcgtctg gttgagggtg
      481 cggatcaggc gcagcacctg gttcaacgcg atcggatcga gcccgacgaa tggctcgtcg
      541 tagaggatca gcatcgggtc gaacacgatc gcccgcgcca gcgccacgcg ccgcgccatg
      601 ccgcccgaga gttcactcgg catcaggttc gccgcgccgc gcaagccgac cgcctggagc
      661 ttggtcagca cgatgttgcg caccaattct tcgcccagcc gcgtgtgctg gcgcagagga
      721 aacgcgacat tctcgaatac gctgaagtcg gtcagcagcg cggagttctg gaacaggaag
      781 ccgatccgtt cgcgcaagcc gaacagcgcg cgccgcgaca gcgtcgtgac ttcctcgcca
      841 tcgacccgca cgcttcccgc atcaggccgc aactgccccg tcaggtgcct gagcagcgtg
      901 gtcttgccgg tgcccgacgg tcccatgacc gcggtgacgc tgccgcgcgg cacctcgagg
      961 tccagcccgt cgaagatcac cttgccaccg agccgtgtgc gcaggtgccg cacctgcacc
     1021 atcggcgcgc tgttgtcgga ttgtgcgtgg gcttgcggca taagcgcgct acgttagcgc
     1081 aggccaaccc ggcggccaag tcgcttcccc cggtccgcca acacccaatt caggtgtggc
     1141 agtcctgccg cttcagttcg acccggcgat tcttcgcacg attggcgtcc gagttgttcg
     1201 gcaccagcgg gtggctgaag ccataaccgt ggctggtcag ccgatccgct gcaacgccgt
     1261 gtcccaccaa ccaggccctg accgcctcgg cgcgttgccc ggagagcttc atgttgtaag
     1321 ccttgccgcc gacattgtcg gtatgcccgc ccacttccgc cttgtacgcg cgatcggcgg
     1381 tgaacaacgc cagcatctgg ttcagtaccg gctccgattc cggcttgatg ccggccttgt
     1441 tgaagtcgaa attgatgccg tacacctcga gcttgcaggc cctcggcggt ggcccgccga
     1501 tctgcagcgc ggtgagcgaa tacgaaccgg tggattcttc gttgccaccc gtatgctcgt
     1561 agatgtacca gagttcaccc tgcggcacct tctgcgtcag caccgcctgg ttggcgtctt
     1621 cggggtcgga cacgagtttg gcgccgatct tctccagcgc attgacgtaa ttcttctgca
     1681 cgtagagcgc cgacgcatcc ttcttctcgt cgatgagcgt gtagttcaat tcgaggaaac
     1741 gcccggcgag tgttttctgg ccggaaggca ggtccagcgt gatcgaatcg agatcgcgct
     1801 tgctgcaatc ggtgagcttg tagtagtcga actgcttcac cagccatggc gggctcttgc
     1861 aacccttgcc ctgcatgtcg tccagcagcg cgccctgcgg cagttgcgcc accaccacct
     1921 gcgggaacgg cgccacgtcc accgtggtca gcgaatacgc gcccgtggat tcctcgttgc
     1981 cgctgccgtg tttgtagagg taccagaact cgcccgccgg cgtcttctgc atcagcgtgg
     2041 cttcgaaccc gttcgtggga tcgctcatga gctttgcgcc gatcttccga agcgcggcga
     2101 tgtagttctt ctgcaccgcg aaagcgaccg ggtccttgtt ttcatcggtg agcttgtagt
     2161 tcaccgtgag ccggcgccct tccagagtct tttcgcccga aggcaggtcg agcttcacct
     2221 gatcccaggc cttggcctcg cacgagtccg ccttgaaata cgcgaactgt tccatcagcc
     2281 agggcggatc gccgcatgcc ccgcccggcg ccggaagcga agctttcatc ggctgcgcga
     2341 ttacggcctg ctgcagtggc acgatcttcc atgtagtcaa cgtataggac gtggtggatt
     2401 cgtcgttgcc gctgccgtgc gtgtagacgt accagtattc gccttgcggc gtcttctgcg
     2461 taaggatggc tttccaactg tcggtgggat cggaggcaag tgtcgccccg gcccgcttgc
     2521 cggcggcgat atggaaatcg cgcgccgccg cggcgctcgg gtccttgctc tggtccttca
     2581 gcgcgtagtt cacttccgtg cgccggcccg cgatcgtctt gctgccggca ggcagatcca
     2641 gatccgtcga atcccaggcc ttgctgtcgc aggaggcaat cacgaaaccc ggcattgacg
     2701 aaggcgccca cgccggagcc ttgcatgggg agacctggcc cgcctgcgca cgcggtatcc
     2761 atgcgatcgc ggcaaccatc agcacgacga atccgagcgc gcgcacggga ccaaaacgca
     2821 tggtcattct ccccgggatg gacaaccggc gacccccgcc gtcgcagcag cttaccgcaa
     2881 agtcagccct gcccgagcaa ctcggcaagc aagcccgggt gcccgtcctg cggcagcggc
     2941 tcgcccgacc gcaccgcatg cacgatcgcc tgcaactgcg ccagcgcgtc ctcgaacacg
     3001 tcgttgacga tcacgcaatc gaattcgtgc gcgtgcgaca tttcctcgcg cgattcggcc
     3061 agccgccggg cgatggtcgc ctccgcgtca gacgcccgcg cgcgcaggcg gcgttccagc
     3121 tccgcgcacg atggcggcag gatgaagatg gacacgcatt ccggcatggc ccggcgcacc
     3181 tgcgccgctc cctgccagtc gatttccagc agcacatcgc gccccgccgc gagcaccggc
     3241 tcgaccgcgc cccgcgaagt gccataacca ttgccgaaca cctcggcgtg ttcgaggaac
     3301 tctccttccg ccaccatctt cgcgaaggtc gcgcggtcca cgaagtggta ctgcacgccg
     3361 tccacgtcgc ccggccgcgg ggcgcgcgtg gtgtgcgaaa tcgacaacga gataccgggc
     3421 tcgcgcgcga gcaacgcgtt caccagtgtg gatttgcccg cgcccgaagg cgccgcaacg
     3481 acaaacaacc tgccggtggc cgacgcgcgg tcactcatgc ggcagggttc ccatggatcg
     3541 cgggacggcc gatgcacaac gcggcagcgt ccctcgaaat gcaaaacagg tgcgatgaaa
     3601 ctccggcatc gcggaagtat agccaatgcg cgaaactgcc cacccggcgt cgacgcaagc
     3661 cttcacacgg cgccagcaag cgttcgcacc ggacgaacgc ccggccgact gctgccgttg
     3721 ctaccgcggc gccaggccgc agcaatcaat agaacccctt gcgtacctgc acccacaatc
     3781 gcgtcccggc gtcggcagcg tccagcaatg ccggcgtggc gccgatggga ctggatgcgc
     3841 tgacatcgac aaaccattcg cccggcgtcg cccagtgaag gccgacgcca gcgctgctgg
     3901 cgcgaccgct gttcacgccg ccgacgaagg cgtccttgta gacctcgacg cggccggtat
     3961 cggcaaacaa cgtggcctgc cagcgccccg gcatgctggg cagatggaaa tcgtggcgaa
     4021 attcggcggt cagaagatcg ccttgcgagc cggacagcgc gcccgtgtca tagccgcgca
     4081 ccgtgtccgg cccgccaagg aagaactgcg cggacgtgtc caggtttttg ctggcccact
     4141 ggcggttgta gcccacgtag atcgcgttgg ccggatcgag ttgctgcagg cgcgacaccg
     4201 atacgacgta acgcgaatac cctcctgcgg tacgcgccgt cagttgatcg atgacttcgg
     4261 cgaggctgtc ggtgaagtcg accttgccgt gcgagacgcc gatgcggaaa ttggtgatgc
     4321 catggacatc acgctggtcg ccggcgagca tcgccgaccc tacgtcgaaa tggcgatcgt
     4381 tgtgcagcag ggcagcctcg atgtcgtcct tcagcaactt tcgatcgaac tcgatctggc
     4441 catagagatt gccggcggtg ctgcggatga acggatgggc caacgccagg ctgccgacga
     4501 gcgccgaccc gtgcgcgtcg agcgcttcca atccgtgtcg caggtggtaa tgcagggtcg
     4561 acacgccagc gcgcaaggtg gtgccctgcc cgccgaggag catccggtag ccgccctgga
     4621 agtagctcat gccgccaccg gaactcgctg caccgagctg cagcaggtca ccacgatgca
     4681 acaggccgtt gacatcgaag ccgccgttga cccgggcgcg accggtatag cggttgccga
     4741 aatcgtccac gccgatcgtg ccggtataac gcggcgcggc ggtgacatcg accaccagat
     4801 cggaagtgcc gggctggtcg cccggacgca ccacggaact ggccgttgcg ccgggaacgt
     4861 ccgagagcag cagcaggctg cgctccaagg ggcgattcgc gaccggctgc cccggttgca
     4921 gcggcgccag aaccttgcgc agcgtcttgt ccgaaacgct gctgtggttc tgcagcacga
     4981 tctttccgta gcgcgcctcc accacgtcga tcttcaccac gccgtcgcgc agcgtctgcg
     5041 ccggcacgta ggcgcgggcg agcggatagc cggccgcgtg gtaggcctca ctgatgcggt
     5101 cggccaggtc ctgaaggtca cccagcgtga gtctgtgccc ttcgctgtcg gacacgaggg
     5161 cgtgcagtgt gctggtcggc agcagcgtgt tgccggtaat ccggattcgc ttcaccagaa
     5221 aggcgtgcac gtcatgcacc ggtcggcgtt gcacatgcag catccgcagg tcgaggtccg
     5281 aggacggcgc ggactccggc tggcgcggca cctgctgcag cagttccccg gcattcggaa
     5341 gctgcgacgg caccgtggga gcctgctggg cttgcacggt accggcgaga gccagcgaga
     5401 cggtgacggc aaacaggccc ctgcgcaggg agtggaatga cttcatgtcg caggttccgt
     5461 tcgaatgcag gacggaaaat agggagcggg tgcgctgcga catgtcagcg cgctcccttg
     5521 gcaggcatgc gcacgccgcc gtcgaccact ttcagcgcca tgccggaagt gaagtccgga
     5581 cgggtgcggt ggcgctcgat cggtcgatgc agcagcttgc ggttgccggt gaaggaaccc
     5641 tcgccatgct ggatggccaa accgtacggc gtcgcgacgg aagccgggcg aatgggactc
     5701 tgcaaatagg caagcacgcc gttgtcgaca ccgaacgccg aaagttgggc tccggtgatg
     5761 ctggcggtcg tcgtggcgac cgggtcgacc agcacgtagt tggctgcatc ggcgccggtc
     5821 aggcggatcc ccgtcaccgt caccgccttg tcgtcgtcga cgttgctgtc ggagaagttg
     5881 gccgcttcgt ggctgaagcc gacctcgtcg cccgacacca cgccctcgcc cgccagcgtc
     5941 accgtggcgg tggtgttgcc gtcgaacatc ttgtcggtac cagtcgcggt caccgtgacg
     6001 ggacgcggag caatgtccgc caccagtccc attggttgct gcaacacgta gttgccggaa
     6061 tcctcgccat cgatcgtcat ggacgtggtc accgccttgc ctgtaccggc gttcgcatcg
     6121 gcgaacaggc ccgacgcggc gttcccgagg gtgacatcgt cgttaccgag cagccccgca
     6181 aggaccgccc cggaaagcgc gtcgacgcgg cttccgtcgt aggtgcgatc ggtggctttt
     6241 gtgccagtca ccgccagcgt cgccggcgta atcgtgaccc agtcgctgcc gccgaccagg
     6301 gtgtagtcgg aggccaaagt cgtgccattg ccggccaagg tgaaaccagc cgtggaagcg
     6361 aatggttttt gctccgcgac attcttgtcg gtgagggtgc cgacgcccga cagcgtcagg
     6421 cgctcgccat tgacgccgtc cagcacgcca ttgtcgccga acaggctggc gtccgcaccg
     6481 gccgtgctgt cgtagacgcg cgtgcccgac aggttcagca cgaatggcgt gatgtcggcc
     6541 gtggtggtcg ccgtggcgtt gatggtgtag ttgcccgcat cggtgccggt cttggtgatg
     6601 ccactgacgc tcacggtctt gccggtcgcg gcgttggcat cggcgaaggt cgccgtgccg
     6661 tagtcgaagt ccaccgtgtc accggtgagc acgccgctgc tgctcaaggt cgcaatgcca
     6721 gcggtgccgg cgtcgtagac cttgtcctgc
//
LOCUS       DASSNH010000052         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_974581, whole genome shotgun sequence.
ACCESSION   DASSNH010000052 DASSNH010000000
VERSION     DASSNH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974581"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..292
                     /locus_tag="VFK00_01850"
     CDS             20..292
                     /locus_tag="VFK00_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7591731.1"
                     /translation="MNKAELINAIADHADLSKADAGRALDAVTDQIKRALKKGDDVSL
                     VGFGTFVVRKRAARSGRNPRTGDTIKIKASKVPAFKAGKALKDALN"
     gene            652..726
                     /locus_tag="VFK00_01855"
     tRNA            652..726
                     /locus_tag="VFK00_01855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:684..686,aa:Val,seq:tac)
     gene            799..2721
                     /locus_tag="VFK00_01860"
     CDS             799..2721
                     /locus_tag="VFK00_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7591732.1"
                     /translation="MLQAIRNMFKSWIVIAIFGVLVVVAFLFFGIEGYFSQSNATWVA
                     KVDGHEISQQDFTTAFNNYRQQQLNSPGNTMDPADFQKPEVKEAVLKQVVNRQLLLNE
                     NDQLGIKVPDAAVREQIASYPVFQVNGKFDTSSYLAWLAQQRKSADQFEDEVRSDLAT
                     RQLPEAIIATAFATKAEAEAFLRLELQQRDFSFVDLPPPAPTPEQGNVSDAEIAGYYK
                     AHQSEFMTPEQVAVNYIDLDAATMKVTPDLSDKALEARYDKEKARFVSPPQWEVAHIL
                     IKLPPKASAADKQAALAKARKVEALAKAPGADFAKLAEEYSDDLGSKRQGGDLGWLSK
                     GDAGPEFQAALDKMKKGGISDPVLTSDGYHIIDVRDLREGHSQSFAEVRDQLAAEAKK
                     DARKDTYQDVGNKLTDMIYNDPTSLKPAAQKLGLTVQTTPLFGRDGGKSGIAANPKVV
                     KAAFSDMVLVQGNTSDPIDLGDDHMVVIHVDKHIPAAPKPLDQVRDQIHHEIVQQRVD
                     ASAKTLAEKAFGELKGGTSLDTLAKAAGQDVQQKSGVIRNAQGIDPDLVTAVFKLAHP
                     AKGAETKALVPMENGHYALVVLHSVTPGDPSTVPQEAQAFLREQMARAYGGADMQEFI
                     DILRDHAKIELAKQRL"
     gene            complement(2982..3770)
                     /locus_tag="VFK00_01865"
     CDS             complement(2982..3770)
                     /locus_tag="VFK00_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET7591733.1"
                     /translation="MGFLEGKRALVTGILSNRSIAWGIAQAMHREGADLAFTYVDEKL
                     RSRVDEAATQLGSDIVLPCDVSRDQDIETLFENLGKRWNGLDILIHSIGFAPREAIQG
                     EYLDGLNRENFRIAHDISSYSLAALAKAARPMMKGRNGAILTLTYLGAQRALASYNVM
                     GLAKASLEANVRYLAYNLGPDGTRVNAISAGPIKTLAAAGIAGFRKMLDHVEHVAPLR
                     RSVTIEEVGNAAAFLCSDLASGITGEVTYVDAGYNTLGMAGLEL"
     gene            complement(3828..>4415)
                     /locus_tag="VFK00_01870"
     CDS             complement(3828..>4415)
                     /locus_tag="VFK00_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7591734.1"
                     /translation="GAIPDIRISSTSRKHLAKLMALSCIIRDPGRFHVKLPDPVPADR
                     LQAVEVPGGVELRAIADMAGVSESKLQALNPGYLGGRIPSHSPGKLLLPANAAETLAA
                     ALANDASGSIAHADSHGRAPDPGHGPQPPAETSPLPQGTDPASAQREHVVRKGETLWS
                     IAHRYHVTIAELKRWNHLDGDGIRPGEALRVGSQP"
BASE COUNT          794 a         1374 c         1500 g          747 t
ORIGIN      
        1 aacccaagag ggagtctcca tgaacaaagc tgaattgatc aatgcaattg ccgaccacgc
       61 cgatctgtcg aaagccgacg ccggacgtgc actcgatgcc gtgacggacc agatcaagcg
      121 tgcgctcaag aagggcgacg atgtgtcgct ggtcggcttc ggtaccttcg tggtgcgcaa
      181 gcgcgccgcc cgttcgggcc gcaacccgcg caccggagac accatcaaga tcaaggcgtc
      241 caaggtgccg gcgttcaagg caggcaaggc gttgaaggat gcgctgaact gagcttcgcc
      301 ttcggccggc attccgtcgg tcgtgtgcat gttttccggc ggccggcgca tcgcgtcggc
      361 cgccgcgttt cgggaccagg aacacatcgc gcacgtggtc ccgcaagatg tgcggcacat
      421 ggatgtgccg ctcgcggaag ccgcgtcgtg gctgacgcgg gaaaacgtgt cgcggctttg
      481 ccggaattgc cctctccctc cgggagaggg tggcgcggag cgccgggtga gggttcggaa
      541 tcgcgaagcg ccgaatgtcg cgcaagcacc gtaccctcac tccgtgccct ctccggaggg
      601 agagggggag tgataatcgc gctgcgcgcg atgcggtatc gattggagcg ggggtgctta
      661 gctcagcggt agagcgtctc ctttacacgg agagggtcgg gggttcgatc ccctcagcac
      721 ccaccaatcc cccacgcgag cgaccggctt cgcttacaat tgccggttca ttcgattcga
      781 cagtcacggc ggtaccccat gctgcaagcc atccgcaaca tgttcaagag ctggatcgtc
      841 atcgcgatct tcggcgtcct ggtggtcgtt gcgttcctgt tcttcggcat cgagggctac
      901 ttcagccaaa gcaacgcgac ctgggtggcc aaggtcgatg gccacgaaat ctcgcagcag
      961 gatttcacca ccgcgttcaa caactaccgg cagcagcagt tgaattcgcc gggcaacacc
     1021 atggatcccg cggacttcca gaagccggag gtcaaggagg ccgtgctcaa gcaggtcgtc
     1081 aaccggcagc tcctgttgaa cgagaatgac cagctcggga tcaaggtgcc ggacgctgcc
     1141 gttcgcgagc agatcgcgtc gtatccggtc ttccaggtca acggaaaatt cgataccagc
     1201 tcctacctcg cgtggctcgc gcagcagcgc aagagcgccg accagttcga ggacgaggtg
     1261 cggtccgacc tcgccacccg ccagttgccc gaagcgatca tcgccactgc cttcgccacc
     1321 aaggccgaag ccgaagcctt cctgcggctg gaactgcagc aacgcgattt cagtttcgtg
     1381 gacctgccgc cgccggcgcc cacgcccgag cagggcaacg tcagcgatgc ggaaatcgcc
     1441 ggctactaca aggcgcacca atccgagttc atgacgccgg agcaggtcgc ggtcaattac
     1501 atcgatctcg atgccgcgac catgaaggtc accccggact tgagcgacaa ggccctcgag
     1561 gcgcgttacg acaaggaaaa ggcgcggttc gtgagtccgc cgcagtggga ggttgcgcac
     1621 atcctgatca agctgccgcc caaggcgagc gccgccgaca agcaggccgc gctggccaag
     1681 gccaggaagg tcgaggcgct ggccaaggcg cccggcgccg atttcgccaa gctggccgaa
     1741 gaatattccg atgacctggg ctccaagcgg cagggcggcg acctcggctg gctgtccaag
     1801 ggcgatgcgg gtccggaatt ccaggccgcg ctggacaaga tgaagaaggg tgggatttcc
     1861 gatccggtgc tgacctcgga cggttaccac atcatcgatg tgcgcgacct gcgcgaaggg
     1921 cattcgcaga gcttcgccga ggtgcgggac cagctggccg ccgaggcgaa gaaggacgcg
     1981 cgcaaggaca cctaccagga tgtcggcaac aagctcaccg acatgatcta caacgatccg
     2041 acttcgctga aaccggcggc gcagaagctc gggttgaccg tccagacgac gccgctgttc
     2101 gggcgcgacg gcggcaagag tggcatcgcc gcgaacccga aggtggtgaa ggcggcgttt
     2161 tcggacatgg tgctggtgca gggcaacacc tccgacccga tcgacctcgg cgacgaccac
     2221 atggtcgtga tccacgtgga caagcacatt ccggccgcgc ccaagccgtt ggaccaggtc
     2281 cgtgaccaga tccaccatga gatcgtccag cagcgcgtgg atgcctcggc caaaacactc
     2341 gcggaaaagg cgttcggcga gctcaaaggc ggcacatcgc tggatactct ggccaaggcg
     2401 gccggccaag acgtgcagca gaaatccggc gtgatccgca acgcgcaagg catcgatccg
     2461 gacctcgtca cggcggtatt caagctggcg cacccggcga agggcgcgga gaccaaggcg
     2521 ctggtgccga tggagaacgg gcattacgca ctggtggtgc tgcattcggt gacgcctggc
     2581 gatccctcga cggtgccgca ggaagcgcag gcattcctgc gcgagcagat ggcgcgcgcc
     2641 tacggcggcg ccgacatgca ggaattcatc gacatcctgc gcgaccacgc caagatcgag
     2701 ctcgccaagc agcgtctgta acgggcgcct gaagacgaag agacgaaacc ccacctctcg
     2761 tttcgttttc cgtctgagcg agcgcagcgc gtgtcgcgcc cgcttttgta ggttgggttg
     2821 atcccggact tgctccggga gaagcccaaa gcggtatcgg cgctcgaatg gtgccgcagg
     2881 tcgtgaacgg cgggtgggag ttgcgaaccg cgttgggctt cgctgcgctc agcgccaacc
     2941 tgcaagagca ctcgagtcgc gaggattgtt tcccgtcttc ttcacagctc caggccggcc
     3001 atccccaggg tgttgtagcc cgcatcgacg taggtcacct cgcccgtgat gccggaggcg
     3061 aggtcggagc acaggaacgc ggcggcgttg ccgacttcct cgatcgtgac gctgcggcgc
     3121 agcggtgcga cgtgctcgac gtggtcgagc atcttgcgaa agccggcgat gccggcggcg
     3181 gcgagggtct tgatcgggcc ggccgagatc gcgttgacgc gggtgccgtc ggggccgagg
     3241 ttgtaggcga ggtagcgtac gttggcctcg aggctggcct tggcgagtcc catcacgttg
     3301 tacgacgcga gcgcgcgttg ggcaccgaga taggtgaggg tgaggatcgc gccgttgcgg
     3361 cccttcatca tcggccgcgc ggccttggcc agcgccgcga ggctgtagct ggagatgtcg
     3421 tgggcgatcc ggaagttctc gcggttgagg ccgtcgaggt attcaccctg gatcgcctcg
     3481 cgcggcgcga aaccgatcga gtggatcaag atatccaggc cattccagcg cttgccgagg
     3541 ttttcgaata gcgtctcgat gtcctggtca cggctgacgt cgcacggcag cacgatgtcg
     3601 gagccgagct gggtcgccgc ttcgtccacc cggctgcgga gtttttcgtc gacgtaggtg
     3661 aaggcgaggt cggcgccttc gcggtgcatc gcctgcgcga ttccccacgc gatcgagcgg
     3721 ttgctgagga tgccggtgac gagggcgcgc ttgccttcga ggaaacccat gtccgcggta
     3781 ctccgttcgg aaagtcgaag gcgccattgt acgggcgctc cgccgcgcta cggctggcta
     3841 cccacgcgca atgcctcgcc gggacggatg ccgtcgccgt ccaggtggtt ccagcgcttc
     3901 agctcggcga tggtgacgtg gtagcggtgg gcgatcgacc atagcgtctc gcccttgcgc
     3961 acgacgtgtt cgcgctgcgc actggcgggg tcggtgccct gcggtagcgg cgaagtttcg
     4021 gccggcggtt ggggaccatg gccgggatcg ggagcccgcc cgtggctgtc ggcatgggcg
     4081 atcgatcccg atgcatcgtt cgccaacgct gccgccagcg tctcggctgc gttcgccggc
     4141 agcaggagtt ttcccgggct gtggctgggg atacggccgc cgaggtagcc tgggttcagg
     4201 gcctgaagct tgctctcgga tacgcccgcc atgtcggcga tcgcgcgcaa ttcgacgccg
     4261 cccggtacct ccaccgcctg caggcgatcc gccggcaccg gatcgggcaa tttcacgtgg
     4321 aagcgtccgg ggtcgcgaat gatgcaggag agtgccatca gcttggcgag atgcttgcgg
     4381 ctggtcgagc tgatccggat gtccgggatc gcgcc
//
LOCUS       DASSNH010000053         8458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1592446, whole genome shotgun sequence.
ACCESSION   DASSNH010000053 DASSNH010000000
VERSION     DASSNH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592446"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..601)
                     /locus_tag="VFK00_01875"
     CDS             complement(176..601)
                     /locus_tag="VFK00_01875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7591735.1"
                     /translation="MNETIRTASAPAPVGAYPHARRVGDLLFLSGIGPRTPGSNAIPG
                     NVYDGDKLVGHDIVAQARQVFANVRAVLEASDAHWEDLVDVTVFLTDMAGDFEGYNAA
                     WAEYFPDPATAPCRTTLGITALPTPIAIELKCIARAGPA"
     gene            complement(598..2049)
                     /locus_tag="VFK00_01880"
     CDS             complement(598..2049)
                     /locus_tag="VFK00_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591736.1"
                     /translation="MIRFRTTCLAILLFAGITPAASAQLPSAWRERQVEAMDKSDAIM
                     KRARNRNDLLAQYQVLRSAYSSDGSPAFRAIFGQYLSWYQSFIGDYKDAELAFSIQQK
                     REPDDHPSPLTQPGWHAVPALDYLPRLARKYRVVFLNEAHNIALTRTLTVRLLKPLRD
                     AGFDVFAVETLYHSDVAALGKRGYPIAGSGFYTREPIYAEMVRTALKLGYKVVAYEAD
                     PDHTGDAREAQQAENLWKILRGDPDAKIVVNAGYAHIQKEGKFLGAQSMAEHFMEDSG
                     ITPLSIEQTIMIPHQENAMDHPDYGAITQALHPAQPVVFIDKHATPWSLRPGYDASVI
                     FPEERFVHGRPTWLALWGARVPYAVNGTVCHNQWPCMVAARYADEGDDAIPADRMVLD
                     PVSLMTIDDIKVTSGNYSIPTGQLYLRPGHRYRITVSDEQGKIIGGTTIGVGNPSAAA
                     SPGNAPGPDVPSTPCAAGGNTIDTPCRNATTHP"
     gene            complement(2128..2781)
                     /gene="can"
                     /locus_tag="VFK00_01885"
     CDS             complement(2128..2781)
                     /gene="can"
                     /locus_tag="VFK00_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010893400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HET7591737.1"
                     /translation="MPSPQELLENNRRWSEAIRAQDPGFFTRLSKQQTPKYLWIGCSD
                     SRVPANQILGIDPGEVFVHRNISNLVLHSDMNCLSVMQFAVDVLQVEHIMITGHYGCG
                     GVAAALKGDRLGLIDNWLRYIGDTLERHQSLVDAEPPARRHDRLCELNVIEQVANACH
                     TTIVRDAWDRGQQLTVHGWCYGLSDGRIHDLGMDVGHFDALDGMYVQALARTGTAPA"
     gene            complement(2896..4362)
                     /locus_tag="VFK00_01890"
     CDS             complement(2896..4362)
                     /locus_tag="VFK00_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET7591738.1"
                     /translation="MEMLTLANLIDGELVPPAGGAYLEVFEPATGKAYARCPDSDTRD
                     VEAAVTAAERAAPAWAALPNGERAQHLHRLANAVEARLDGLAREESRDSGKPVALARR
                     VDIPRAVANLRFFAEAITQWPGESHADARAVNVTLRQPLGVVGCISPWNLPLYLFTWK
                     IAPALAAGNAVVAKPSEITPFTAFRLSQLAIEAGFPPGVLNIVQGTGPEVGGAIVGHP
                     KVKAVSFTGSTRTGAAIAAECAKTFKKASLEMGGKNPAIVFADADLSDANLDTIVRSG
                     FSNQGEICLCGSRLLVERSIHDDFRQRYLERVRALRVGDPEDAHTDLGALVSKPHFEK
                     VTACIARAREEGGKVLAGGGTVDVPGRCADGWFVAPTVIEGLPQDCATNREEIFGPVV
                     TLIPFDSEDEAVAIANGTDYGLAASVWTRDLARAHRVSERIDFGIVWVNCWMLRDLRT
                     PFGGVKHSGMGREGGVDALRFFTEPKNVCIALQERAGA"
     gene            complement(4476..5264)
                     /locus_tag="VFK00_01895"
     CDS             complement(4476..5264)
                     /locus_tag="VFK00_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7591739.1"
                     /translation="MDTNLAGRHALVCGASQGIGRACAIELAGLGANVTALARRPEVL
                     GQLVEALPRIDDSQKHTFLVADTGDTDGLQSAVQELAARRAVHILVNNSGGPPPGPVH
                     GAEIEAFLDAYRKHLVANHVLAETVIPGMQAAGYGRIVNIISTSVKEPIRGLGVSNTT
                     RWAVASWAKTLATELAPFGITVNNVLPGATRTPRIEQIVATRAQKSGNSTADTQRAME
                     AETPMQRFADPSEIAAAVAFLASPAASYITGINLPVDGGRTRSL"
     gene            complement(5286..6077)
                     /locus_tag="VFK00_01900"
     CDS             complement(5286..6077)
                     /locus_tag="VFK00_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591740.1"
                     /translation="MPSFLRTLLALLAIGSLAGCTHAIKLPLFSDSAPPPSRDMALQR
                     LKDATPCCHAWADLPFHDQLPAEPREFTIDKFSPVADIDGNRTHFLTFDLPKFKKPYR
                     VVFQTQPSARHLGNSFLLAPTVTLLNADYQPLSSTDVPLCVYINWRPSMSGGFGAVTI
                     DNPNAQYLVVTTSTQQLAATTYWAQSPTSFSNVNLPSASALASIRPEVPATSGTFDVQ
                     HGPEGTLYLGKMTPAYASAVDNGLCGRPKTGAGLLPELRQALQNL"
     gene            complement(6210..7610)
                     /gene="asnS"
                     /locus_tag="VFK00_01905"
     CDS             complement(6210..7610)
                     /gene="asnS"
                     /locus_tag="VFK00_01905"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET7591741.1"
                     /translation="MATLSVQQALAGGTPEGEAVEVRGWVRTRRDSKAGLSFVNVSDG
                     SGFATLQVVAPASLPNYASEVQRLSAGCAVVARGKLVPSQGKGQAFELQADSIEVTGW
                     VDDPETYPIQPKQHSPEFLREVAHLRPRTNLFGAISRVRHTMMTAIHRHLTAEGFFWV
                     NTPIITTSDAEGAGDMFRVSTLDLANLPRDDKGAIDFRQDFFAREAFLTVSGQLNVEA
                     YCLALSKVYTFGPTFRAENSNTPRHLAEFWMIEPEIAFADLADNADCAEKFLKAIFRA
                     VLDERADDMAFFAERVEKDAIGRLEKFIAAPFERIEYTDAIEILKKSGQKFEFPVEWG
                     VDLQTEHERWLAEQHIGRPVVVMNYPEQIKAFYMRLNDDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDHLDRRMQQFGLDPAHYQWYRDLRRYGTVPHAGFGLGFERLLVYVCGLSN
                     IRDAIPYPRTPGHAEF"
     gene            7698..8045
                     /locus_tag="VFK00_01910"
     CDS             7698..8045
                     /locus_tag="VFK00_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET7591742.1"
                     /translation="MSIELTPNASARMRDFLGKHPSAPGVRFGIKRTGCSGFGYTVDL
                     ASEVTGDDKVFDFDGIKLVVDANALPFVDGTRIDYARDGLNAGFVFHNPNATAACGCG
                     ESFTVEQQGIGGG"
     gene            8285..>8458
                     /gene="rpsF"
                     /locus_tag="VFK00_01915"
     CDS             8285..>8458
                     /gene="rpsF"
                     /locus_tag="VFK00_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7591743.1"
                     /translation="MRHYEVVFLVHPDQSEQVPGMLDRYKALVEGEGGKVHRVEDWGR
                     RPLAYPIQHLAKAH"
BASE COUNT         1351 a         2708 c         2917 g         1482 t
ORIGIN      
        1 catgcaggcc ttcgatgccg ccggcatcgg catcgcctcg gcgacctacg acatcgtcgg
       61 gctgccgccg gtccgggttc gccgcgaaaa ggacccggaa tagggatcgg ggggaaccgg
      121 cgaagccgtt tccaccgatg cggctgttgg cggacgccgc tgcacgggtt ccgacctacg
      181 cggggcctgc ccgcgcgatg cacttcagct cgatcgcaat cggcgtcggc agggcggtga
      241 tgccgagggt ggtgcggcag ggcgccgtgg ccgggtccgg gaagtactcc gcccaggcgg
      301 cgttgtagcc ctcgaagtcg cccgccatgt cggtgaggaa tacggtgacg tccaccaggt
      361 cctcccagtg cgcgtccgaa gcttccagca cggcgcgcac attggcgaac acttgccgtg
      421 cctgcgccac gatgtcgtgc ccgaccagct tgtcgccgtc gtaaacgttg ccggggatgg
      481 cgttgctgcc gggcgtgcgc gggccgatgc ccgagaggaa gagcaaatcg cccacgcgcc
      541 gtgcatgcgg gtaggcgccg accggtgcgg gcgcggatgc ggtgcggatc gtttcgttca
      601 tggatgtgtc gtggcgttgc ggcaaggcgt atcgatggta ttcccgcccg cagcgcaggg
      661 cgtggacggc acatcaggcc caggcgcgtt tcccggggaa gcggcggccg aagggttgcc
      721 caccccgata gtggtgccgc cgatgatctt tccctgctcg tcgctgacgg tgatccggta
      781 gcgatggccg ggccgcaggt aaagctggcc ggtggggata ctgtaattgc cgctggtgac
      841 cttgatgtcg tcgatggtca tcagcgaaac ggggtcgagc accatgcggt cggccggaat
      901 cgcgtcatcg ccttcgtcgg cgtaacgcgc cgcaaccatg cacggccact ggttgtggca
      961 gaccgtgcca ttcaccgcgt agggcacgcg ggcgccccac agcgccagcc aggtcgggcg
     1021 cccgtggacg aagcgctctt cgggaaagat cacgctggcg tcgtagccgg gacgcagcga
     1081 ccacggcgtg gcatgcttgt cgatgaagac gaccggttgc gccgggtgga gtgcctgggt
     1141 gatcgccccg taatccggat gatccatcgc gttctcctgg tgcgggatca tgatggtttg
     1201 ctcgatcgag agcggcgtga tgccgctgtc ctccatgaag tgctccgcca tggattgcgc
     1261 gcccaggaat tttccctcct tctggatgtg cgcgtagccg gcgttgacca cgatcttcgc
     1321 gtccggatcg cccctgagga tcttccagag gttctccgcc tgctgcgcct cgcgtgcatc
     1381 gcccgtgtgg tcgggatccg cttcgtaggc gaccaccttg tagccgagct tcagtgcggt
     1441 ccgcaccatt tccgcgtaga tcggttcgcg cgtgtagaag ccgctgccag cgatcgggta
     1501 tccgcgcttg ccgagcgccg cgacatccga gtggtagagc gtttcgaccg cgaacacgtc
     1561 gaagcccgcg tcccggagcg gcttgagcag tcgcacggtg agggtgcggg tcagcgcgat
     1621 gttgtgcgcc tcgttgagga acaccacgcg atatttcctc gcgagtcgcg ggaggtaatc
     1681 gagcgcgggc accgcgtgcc agcccggctg cgtcagcggt gatggatggt cgtcgggttc
     1741 gcgcttctgc tggatcgaaa acgcgagctc ggcatccttg tagtcgccga tgaagctttg
     1801 gtaccaggaa aggtactggc cgaagatcgc gcggaatgcc gggctgccgt cggacgaata
     1861 ggcgctgcgc agcacctggt attgcgcaag caggtcgttg cggttgcgcg cacgcttcat
     1921 gatcgcgtcc gacttgtcca tggcttcgac ctggcgctcg cgccatgccg aaggcagttg
     1981 cgcggacgcc gccggcgtga tgccggcgaa cagcaggatg gcgaggcagg tcgtgcggaa
     2041 gcggatcatg gcgtgcgcgg ctggcggcgc cgggattccg cggctatggc cgaggtcgcc
     2101 ccggagcgtc gcctcgcagc gtatcactca cgccggagcg gtgcccgtcc tcgccaaggc
     2161 ctgcacgtac atgccgtcca gcgcgtcgaa atgcccgaca tccattccga ggtcgtggat
     2221 gcgaccatcc gaaagcccgt agcaccagcc gtgcacggtg agttgctgac cgcggtccca
     2281 ggcgtcgcgc acaatggtgg tgtggcaggc gttggctacc tgttcgatca cgttgagttc
     2341 gcacagacgg tcgtggcggc gggccggagg ctcggcatcc accagggact ggtggcgttc
     2401 cagcgtatcg ccgatgtagc gcaaccagtt gtcgatcagg ccgagccggt cgcccttgag
     2461 cgcggcggcc acgccaccgc agccgtaatg cccggtgatc atgatgtgct ccacctgcaa
     2521 cacgtccacc gcgaactgca tcaccgacag gcagttcatg tccgaatgca ataccaggtt
     2581 ggaaatgttc cggtgcacga atacctcgcc ggggtcgatg ccgaggatct ggttggcggg
     2641 tacgcgcgag tcagagcagc cgatccacag gtacttcggc gtctgctgct tggacagcct
     2701 cgtgaagaat cccggatcct gcgcgcggat cgcctccgac cagcggcggt tgttctcgag
     2761 cagctcttgt ggtgacggca tggtcggtcg ttctccggat gaaaacaatg tagcgaattg
     2821 taggagcggc ccaccgccgc gatcaggcga ttcgtgtcac cgcgaatccg ttggcgcacc
     2881 aggcaaaaga cggcgctacg cgccggcgcg ctcctgcaat gcgatgcaca cgttcttggg
     2941 ctcggtgaag aatcgaagcg cgtcgacacc accctcgcgg cccatgcccg aatgcttgac
     3001 gccgccgaac ggcgtgcgca ggtcgcgcag catccagcag ttcacccaca cgatgccgaa
     3061 gtcgatccgc tcggagacgc ggtgcgcccg cgcgaggtcg cgcgtccaga ccgaagccgc
     3121 caggccgtag tccgtgccgt tggcgatcgc aacggcttcg tcttcggaat cgaacgggat
     3181 cagcgtcacc acgggcccga agatttcctc gcggttggtc gcgcaatcct ggggcaatcc
     3241 ttcgatcacc gtgggcgcca cgaaccagcc gtccgcgcaa cggccgggca cgtccacggt
     3301 cccgccgccg gcaagcacct tgccgccctc ctcgcgcgcc cgcgcgatgc acgccgtcac
     3361 cttttcgaag tgcggcttcg acaccagcgc gccgaggtcg gtgtgcgcgt cttcggggtc
     3421 tcccacgcgc agcgcacgca cccgttcgag atagcgttgc ctgaaatcgt catggatcga
     3481 acgttcgacc agcagccggg aaccgcacag gcagatttcg ccctggttgg aaaaacccga
     3541 gcgcacgatg gtgtcgaggt tcgcgtcgga cagatcagcg tccgcgaaga cgatggccgg
     3601 attcttgccg cccatctcca gcgacgcctt cttgaacgtc ttcgcgcatt ccgcggcgat
     3661 cgcggcgccc gtgcgcgtgg accctgtgaa cgacaccgcc ttgaccttgg gatgcccgac
     3721 gatcgcaccg ccgacttcgg ggccggtgcc ctgcacgatg ttcagcacgc ccggcggaaa
     3781 gccggcttcg attgccagtt gcgacaatcg gaaggcggtg aagggcgtga tttcggaggg
     3841 tttggccacg accgcgttgc cggccgccag cgccggtgcg atcttccagg tgaagagata
     3901 aagcggcagg ttccatggcg aaatgcagcc gacgacgccc agtggctggc gcagcgtgac
     3961 attgaccgcg cgggcatccg cgtgcgattc ccccggccat tgggtgatgg cctcggcgaa
     4021 gaagcgcagg ttcgcgaccg cgcgcgggat gtccacgcgg cgcgcgagcg cgaccggttt
     4081 gccggagtcg cgcgattcct cgcgggcaag cccgtcgagc ctcgcttcca ccgcattggc
     4141 cagtcggtgg aggtgttgtg cgcgttcacc attcggcagc gcggcccacg ccggcgcggc
     4201 acgttcggcc gcggtgaccg cggcttccac gtcgcgcgta tccgaatccg ggcaacgcgc
     4261 gtacgccttg ccggtcgccg gttcgaacac ctccaggtag gccccgcccg ccggcggcac
     4321 gagttcgcca tcgatcaggt tggccagcgt gagcatttcc atcgcgcaag cttagccgat
     4381 aggcgcttcc gccagcctcg aaacaccgat tccggcgcaa accgctacgc gggttatgcc
     4441 ctacctcggc accaatcgac tctcacggag cggggttaca gcgagcgcgt gcgtccgccg
     4501 tcgaccggca ggttgatgcc ggtgatgtag cttgcggcgg gcgaggcgag gaacgccacc
     4561 gcggcggcga tttccgaagg atcggcgaag cgctgcatcg gggtttcggc ttccattgcg
     4621 cgctgggtgt cggcggtgct gttgccggat ttctgcgcgc gggtcgcgac gatctgctcg
     4681 atgcgcggag tccgggtcgc gccgggcagc acgttgttga cggtgatgcc gaacggcgcg
     4741 agttcggtcg cgagggtctt ggcccagctc gccaccgccc agcgggtggt gttggacacg
     4801 cccaggcccc ggatcggttc cttcaccgag gtcgagatga tgttcacgat ccgcccgtag
     4861 cccgccgcct gcatgccggg gatcacggtc tcggcgagca cgtggttggc gacgaggtgc
     4921 ttgcgatagg cgtcgaggaa cgcctcgatt tcggcgccat gcacgggccc cggtggcggc
     4981 cccccggaat tgttgacgag tatgtgcacg gctcgccggg cggcaagttc ctgtactgcg
     5041 ctctgcagcc catcggtgtc accggtgtcg gcgacgagga acgtgtgctt ctgcgagtcg
     5101 tcgatgcggg gcagcgcctc gaccagttgc ccaagcacct cggggcggcg cgccagtgcg
     5161 gtgacattgg cgccgaggcc agccaattcg attgcacaag cgcggccgat cccctgcgag
     5221 gcgccacaca ccagcgcgtg gcggccggcg agattcgtgt ccatcgggtg ctccgcgggg
     5281 aggagtcaga gattttgcag tgcctggcgt agttcgggca acagccccgc gcctgtcttc
     5341 ggccggccac aaagtccgtt gtcgaccgcg ctggcgtagg ccggcgtcat tttcccgaga
     5401 tagagcgtgc cttcgggccc gtgctgcacg tcgaaggtgc cgctggtcgc cggcacctcc
     5461 ggccggatgc tcgcgagcgc cgaggccgac ggcaagttga cgttgctgaa gctggtcggc
     5521 gactgtgccc agtacgtggt ggccgccaat tgttgcgtgg acgtggtcac cacgaggtat
     5581 tgggcattgg ggttgtcgat ggtcaccgcg ccgaacccgc ccgacatgct ggggcgccag
     5641 ttgatgtaga cgcacagcgg cacgtcggtg ctggaaagcg gttggtaatc cgcattgagc
     5701 agggtgaccg tcggcgccag caggaagctg ttgccgaggt gccgggcgct cggctgggtc
     5761 tggaacacca cccgataggg cttcttgaac ttcggcaggt cgaaggtcaa gaaatgcgtg
     5821 cggttgccgt cgatgtccgc caccgggctg aacttgtcga tggtgaactc gcggggctca
     5881 gcgggcagtt ggtcgtggaa cggcaggtcg gcccaggcgt ggcagcaggg cgtggcgtcc
     5941 ttgaggcgct gcagggccat gtcccgcgaa ggcggcggcg cgctgtcgga gaacagcggg
     6001 agcttgatgg cgtgcgtgca gccggcgagc gagccgatcg ccagcagggc caggagggtg
     6061 cggaggaaac ttggcacgac ggaagcgttg tcagcggtcg ggaggccaga tgaacaaagc
     6121 ctgaaagcgt agtgcggaaa tctgacgatt tggtggcctg tcaagggcgg gatgcgtgac
     6181 cggctaccca gttcgacacc ctgcgctgct cagaattccg catgtcccgg ggtgcggggg
     6241 taggggatcg cgtcccggat gttggacagg ccgcagacgt acacgagcag ccgctcgaaa
     6301 ccgaggccga agcccgcgtg cggcaccgtg ccgtagcgcc gcaggtcgcg gtaccactgg
     6361 tagtgggccg ggtcgaggcc gaactgctgc atccgccgat cgagatggtc caggcgctcc
     6421 tcgcgttgcg aaccgccgat gatctcgccg atgcccggcg ccagcacgtc catcgcggcg
     6481 acggtcttgc cgtcgtcgtt caggcgcatg tagaacgcct tgatctgctc ggggtagttc
     6541 atcacgacca ccggccggcc gatgtgttgt tccgcgagcc agcgctcgtg ttcggtctgc
     6601 aggtccacgc cccattccac cgggaattcg aacttctggc cggacttctt caggatttcg
     6661 atggcatcgg tgtattcgat ccgctcgaac ggcgcggcga tgaacttctc gagccggcca
     6721 atggcgtcct tctccacgcg ttcggcgaag aacgccatgt cgtcggcgcg ctcgtccagg
     6781 accgctctga agatcgcctt caggaacttc tccgcgcaat cggcgttgtc ggcgaggtcg
     6841 gcgaacgcga tttcgggctc gatcatccag aactcggcga ggtgccgcgg cgtgttggag
     6901 ttttcggcgc ggaaggtcgg accgaaggtg tagaccttcg agagcgcgag gcaatacgcc
     6961 tccacgttca gctggcccga gaccgtgagg aacgcttcgc gggcgaagaa gtcctgcctg
     7021 aagtcgatcg cacccttgtc gtcgcggggc aggttggcga ggtccagggt cgagacccgg
     7081 aacatgtcgc cggcgccctc ggcatcggac gtggtgatga tcggggtgtt cacccagaag
     7141 aagccctccg ccgtcaggtg gcgatggatc gccgtcatca tcgtgtggcg cacccgcgag
     7201 atcgcgccga acaggttggt acggggacgc aggtgcgcga cttcgcggag gaattcgggc
     7261 gagtgctgct tgggctggat cgggtaggtt tccgggtcgt ccacccaacc ggttacctcg
     7321 atcgagtcgg cctgcagttc gaacgcctgg cccttcccct gcgacggcac cagcttgccg
     7381 cgcgcgacga cagcgcagcc cgccgagagc ctctgcactt cgctcgcata gttgggaagc
     7441 gaggccggcg cgaccacctg cagcgtcgcg aagccggaac cgtcggatac gttgacgaac
     7501 gacaatccgg ccttggagtc gcggcgcgtg cgcacccagc cgcggacctc gacggcttcg
     7561 ccctcgggcg tgccgccagc gagtgcctgt tggacgctca gggttgccat ggcttgaact
     7621 cgtgccctcg atggaccatg taatgtaagg acgaacgcgc gataataact tactagtaca
     7681 cgcaacagat attcttcatg tccatcgaat tgactccgaa cgcctccgcg cgcatgcgcg
     7741 atttcctcgg caagcacccg tccgcgccgg gcgtgcgctt cggcatcaag cgcaccggct
     7801 gctccggctt cggctacacc gtcgatctcg ccagcgaggt gaccggcgac gacaaggtgt
     7861 tcgacttcga cggcatcaag ctggtggtgg atgccaatgc cctgccgttc gtggatggca
     7921 cccgcatcga ctacgcgcgt gacggcctga atgccgggtt cgtattccac aaccccaatg
     7981 ccactgcggc ctgcggttgc ggggagagct tcacggtgga gcagcagggg attgggggcg
     8041 ggtagcgggg cccggttttc gggctctgtg cagattccaa ccgctcggac ccgttcgagc
     8101 gcgccgatgc cacccatccg ccggccccgc ttctgcttgc gccacagcgc cgttccccgt
     8161 agaatttcgc ctcttgcgtc cacggcccag gccgaacgca agccctcgcc tcaccgccgg
     8221 tcggaatggt cgggaggctg ccccgccatg gcccgcgccc tggcgacacc cgtgaggaat
     8281 cacaatgcgt cattatgaag tcgtgttcct ggtccacccg gaccagagcg agcaggtgcc
     8341 cggcatgctc gaccgctaca aggccctcgt cgagggcgaa ggcggcaagg tgcaccgcgt
     8401 cgaggactgg ggccgccgcc cgctcgccta tcccatccag catctggcca aggcccac
//
LOCUS       DASSNH010000054        18600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_455028, whole genome shotgun sequence.
ACCESSION   DASSNH010000054 DASSNH010000000
VERSION     DASSNH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 18600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18600
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455028"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VFK00_01920"
     CDS             complement(<1..1066)
                     /locus_tag="VFK00_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HET7591744.1"
                     /translation="MPRRLVALFDGTWSKAETLTNVERFWKLVAPVDGAGHEQVCKYF
                     AGVGVKAGIEHWLGGAFGLGLSGNVEDGYQWLSKTWQEGDEIWLLGFSRGAYTARSLG
                     GLIRKCGLLNPDDKGDVPRSDVDAAYALYRNNLHPNDPAMQAFRAAHSREVLIRFIGV
                     WDTVGALGIPGVGSWFPFSRSRYAFHDTDLSKIVQYAYQALALDEHRADFKPTKWTRP
                     AGTLAPGESHTVWKPEQIEVEQRWFIGAHSDVGGGEKTDGAGHSPDTLPEIPLAWMQA
                     KAAGAGLAFSEKFVPAAGAELDVPNDSYASFMGGLYKLFSKPYERILGGGIRETIDPS
                     VWMKWYEQAVYRPPSLEVAMD"
     gene            complement(1220..2695)
                     /gene="purF"
                     /locus_tag="VFK00_01925"
     CDS             complement(1220..2695)
                     /gene="purF"
                     /locus_tag="VFK00_01925"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7591745.1"
                     /translation="MCGIIGIVGKTEVATALYDALTVLQHRGQDAAGIATFDGSKLHL
                     RRGPGLVRDVFSARDMQELRGHAGIGHCRYPTAGAEDSEEAQPFYVNSPYGIAFAHNG
                     NLINTEQLRREMFENDRRHINTASDSEILLNILAHELQIQDRHALEPDELFKAVAAVH
                     ARAKGGYACVAMILGYGVLAFRDPHGIRPLVLGERDTPEGKEYACASESVAFDILGFR
                     RVRDVAPGEAVFMGFDGTLSARRCAEGAVHAPCIFEYVYLARPDSMIEDVSVYKARLR
                     MGEKLAAKILRERPDHDIDSVIPIPDTSRTAGSTLATALGVPFREGFVKNRYIGRTFI
                     MPGQGQRVRSVRRKLNPIPLEFRNKNVLLVDDSIVRGTTSRQIVQMARDAGANKVYFA
                     SAAPPVRYPNVYGIDMPAPAELVAHGRSLEDIRETIGADWLVYQDLPDLIEAVAEGNH
                     KLKHFDDSCFSGCYVTGVEPEYLRQLEFARSDEAKAQRRSA"
     gene            complement(2846..3469)
                     /locus_tag="VFK00_01930"
     CDS             complement(2846..3469)
                     /locus_tag="VFK00_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7591746.1"
                     /translation="MNTADILILAVLALSMLYGVWRGFISEVLSLACWIAAFWVAWAF
                     GDEVAAFYGGFLREPTACIVAGYLTCFLGVLIAGALLGWMVRRLLSHGGLRGGDRLLG
                     LVFGFARGVLLVTFAVLMLGFTSVPRQAAWWRQSTLLPAFEGGAGWFAERLPAEVSHY
                     LEIGGQALPALSQIPISTLQKQAGPPAASVPPGRAPGHGPDRRDVGQ"
     gene            complement(3484..4443)
                     /locus_tag="VFK00_01935"
     CDS             complement(3484..4443)
                     /locus_tag="VFK00_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7591747.1"
                     /translation="MDSGLKKRLLGAAVLIALAVIFVPMLLPSRSGTEPLSTDVPPPP
                     SSELQTRVLQVGPDSASAGSSTAASIVDPDRVASLDLNGGTHQTATVAPGATAVTPLA
                     ASSAAETEATGAAAASSTKVASAEPKPISSAPEPAEPVAGGAGAAAGTVYTVNLGIYA
                     DHGSARRLVADAKRHGFTAVATPETFRGKSVLRVRVGPFSSKAAAEAARLKLKDFEHV
                     AMSVDTASVDKQGDAPASALPASTPGGWAVQLAAFSDQSSAENLRDRLRSQGFDGYVD
                     SVKTSKGTLWRVRAGPFATREVAESARGRIADEIKIKGNVVTE"
     gene            complement(4579..5919)
                     /gene="folC"
                     /locus_tag="VFK00_01940"
     CDS             complement(4579..5919)
                     /gene="folC"
                     /locus_tag="VFK00_01940"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7591748.1"
                     /translation="MKTLDEWLAYQQTIHARGIDMGLDRVDEVWRRMGAPRPAPLVVT
                     VGGTNGKGSVVAFLEAMLRAGGMRVGSYTSPHILRYNERVRVDGVEARDASLVEAFER
                     VEAARIGDGSATIPLTYFEFGTLAALGVFARAELDVALLEVGLGGRLDAVNIVDADCA
                     VVVTVDLDHMEYLGSDREAIGREKAGIFRAGKPAIVGETNPPASLLDHAHAIGARLEV
                     LGRDFGWSPETETLVWWHRDPAQVRYVAPAEGHAGGVALHGELALEGAFQYRNAAAAI
                     AALHALGERVRWSAAAIEKDAFARTPSASPFLAGRLQHLASAPDLVVDVGHNPQAARE
                     LARWLDRHPVPGSTRAVFGALADKDIEAVATALADRVDHWYLAGLDGESPRGEPIERL
                     AARFRAAAPAASCSEHPDVTSALAAARAEASADGRIVAFGSFHVVAPVLRACPV"
     gene            complement(6048..6911)
                     /gene="accD"
                     /locus_tag="VFK00_01945"
     CDS             complement(6048..6911)
                     /gene="accD"
                     /locus_tag="VFK00_01945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET7591749.1"
                     /translation="MSWLQKLTSPNIRTEGAPVKGKVPEGVWEKCSGCGIGLYRPELE
                     RNLMVCPKCGYHHPIGARARLAAFLDEGGNELDAGMGPVDSLKFKDSKKYRDRILAAQ
                     KATGEKDALIAVQGALKGLPAIACAFEFSFMGGSMGSVVGEKFTRAAEAALRNKTPFV
                     CFTATGGARMQEGLQSLLQMAKTSAALARLRDAGLPYIAVLTHPTTGGVSASLAMLGD
                     IQIAEPRALIGFAGPRVIEQTVREKLPEGFQRSEFLLEHGAIDMICDRREIRDRIAAL
                     LAILVHRDKVA"
     gene            complement(7007..7804)
                     /gene="trpA"
                     /locus_tag="VFK00_01950"
     CDS             complement(7007..7804)
                     /gene="trpA"
                     /locus_tag="VFK00_01950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7591750.1"
                     /translation="MAAVFAALKQRSRAALIPFITAGDPFPGATVEVMHALVEAGADL
                     IELGMPFSDPMADGPVIQHADERALRNKVGVGGILRWAREFRERDAETPLVLMGYLNP
                     IAIRGIERFAREAVEAGVDGVLVVDCPIEEDATLQPLRDAGLQRILLAAPTTSDARLA
                     RAIEAAEGYLYYVSFAGVTGAAKLSTGDIAQRVGAIRERAKVPVAVGFGIKDASSAAA
                     VAAFADAVVIGSALVGSLAHANDAGDACRRARDFLAPIRSALDGTRA"
     gene            complement(7855..9066)
                     /gene="trpB"
                     /locus_tag="VFK00_01955"
     CDS             complement(7855..9066)
                     /gene="trpB"
                     /locus_tag="VFK00_01955"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7591751.1"
                     /translation="MKEAIDYSRYPDANGRFGPYGGTYVAETLIAPLGELTAAYTRLR
                     DDPAFQNEFADDLRQYVGRPSPIYHAKRLSERVGGAQILLKREDLNHTGAHKINNTVG
                     QALVARAMGKQRIIAETGAGQHGVATATVCARFGMKCVVYMGAVDIERQKINVYRMKL
                     LGAEVVPVTSGSKTLKDALNEALRDWVTNVADTFYIIGTVAGPHPYPMMVRDFNAVAG
                     REARAQMLEQFGRLPDVLTACVGGGSNAIGLFHAFLNDRDVRIIGAEAAGEGIASGHH
                     AASLAAGRPGVLHGNRTYVLCDDDGQITETHSVSAGLDYPGVGPEHAFLKDTGRAEYV
                     GVTDKEALAAFHLLCETEGILAALESSHAVAQAIKLARGLPKDKLILCNLSGRGDKDV
                     HTIAEREGFEL"
     gene            complement(9153..9770)
                     /locus_tag="VFK00_01960"
     CDS             complement(9153..9770)
                     /locus_tag="VFK00_01960"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7591752.1"
                     /translation="MKICGVTRVEDAVLACELGADAIGMVMTPSSPRCVSIERASAIR
                     DALPAFVDAVVLSHDLPADRVRQIIDGVQPDLVQFHGTEGAAFCESFGLRYLKALGMA
                     GDLDVRAVAAEHARAAGFVLDGHPPGQQGGQGRAFDWSRVPQELGRPIILAGGLNAGN
                     VADAIGAVHPWAVDLASGVESAPGIKDAGKLRAFFAAVAEAEAPL"
     gene            complement(9813..10580)
                     /gene="truA"
                     /locus_tag="VFK00_01965"
     CDS             complement(9813..10580)
                     /gene="truA"
                     /locus_tag="VFK00_01965"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017911049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7591753.1"
                     /translation="MRVALGIEYDGTDFFGWQRLGHGPSVQAEVESALSFVADHPVEV
                     TCAGRTDAGVHARCQVVHFDTHAARSERAWILGATSRLPREVAVLWARAVPDDFHARF
                     SARARRYRYAILNRAIRPALDARFLSWERVPLDAGAMHAAAQALVGERDFSSFRAVSC
                     QARHARRNVTEVSVRRDGGRVIIEIEANAFLHHMVRNIVGSLLEVGRGERPAGWMAEL
                     LAARDRNVAGVTAPPQGLTFLGPKYPSEWSLPPEVTL"
     gene            complement(10621..12846)
                     /locus_tag="VFK00_01970"
     CDS             complement(10621..12846)
                     /locus_tag="VFK00_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HET7591754.1"
                     /translation="MKLKLRIAGAALLALAMSPAWALQLGQIEVKSALNQPLVAAIPL
                     QPKNLSELDGLTVKLASADDFARAGLPLTATDRTLRFHVVTDNNGQKLILVTSSAPVS
                     DPYLDFLIEVNTRQAKQVREFVVLLNPVISAPAPAVQTAPISSPVAAPPPSHPAQLPP
                     PEAAAQPAPPPQAPAPAKPEQQQAAPPPAKPQPQPQAAARKPTPPPAPPKPQPAPQPA
                     AAAPASGTIPVERGDTLYGIAKRVTPDTGTTVNQMMMALKSANPEAFFKDNINDLKAG
                     AILRIPTREQIDQTSIAEADAAVHRQYEAWRSARPHPATVLEGTAANAAARTAPKPTV
                     AAPETDHLSLVPPSGGASGAGNRPGVAGGTGSETVAGLQQRLQNERGTLASLEQSNAD
                     LDSRMHSLEDLAGKSAKLLSFKDATIAELQRKLAAVQAGKPLAATSSAGVATAAAGNE
                     SGKTAVAKPAGKTAAATSPASATPWFERPLTWIIAAVIALALILVGLLARRRGGTEAE
                     AGVPGPLTDPEDLAPPASTAAYRASLEAQLAENPDDLGAHLALCRLYHAEGDAARFVA
                     AAEAMHAHVREPGSLEWHEVAVMGEELAPGDPLFAAPAEGPAETAVVETEVEEEPEVV
                     VPPQDFAPGEDLEMEPVSEAVEDEPAPESRPEPAPDAWDESLPVMGTITEAHPEPASA
                     ETAAVQFSDDPVDTKLDLARAYLDMGDPDGARAMLDEVLEEGSQTQKDEARRLLAEST
                     D"
     gene            complement(12972..13292)
                     /locus_tag="VFK00_01975"
     CDS             complement(12972..13292)
                     /locus_tag="VFK00_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591755.1"
                     /translation="MRLPKRLMRTGMLCVLMGIGMLLAGPSAVAAESHGDKAVQAQLH
                     DLQAKVSKAQDRNAELQAQVAKMEQQNAERQKQLQQRDQEIAALQRKLEAAGAAPSPA
                     TSGH"
     gene            complement(13443..14471)
                     /locus_tag="VFK00_01980"
     CDS             complement(13443..14471)
                     /locus_tag="VFK00_01980"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7591756.1"
                     /translation="MAKKTQYNLAMVGATGAVGETLLSILEDRAFPVAELVPLASERS
                     AGSKLKFRGKQVTVREVASYDFSGIDIAFFSAGAGVSREHAPRAADAGAVVIDNTSEF
                     RYVDSVPLVVSEVNPHAIAEYTNRNIIANPNCSTMQMLVALAPIHRAAGIERINVATY
                     QSVSGAGRSGMEELGRQTADLLNFREIEPEKFSAQIAFNVIPHIDAFQDNGYTREEMK
                     MVWETRKILEDDAIQVNPTCVRVPVFFGHSEAVHIETREKIDAATARALLEAAPGVVV
                     VDERKPGGYPTPVGAAAGKDAVFVGRIRDDISHPTGLDLWVVSDNIRKGAALNAVQVA
                     EILIREYL"
     gene            complement(14720..15817)
                     /gene="aroC"
                     /locus_tag="VFK00_01985"
     CDS             complement(14720..15817)
                     /gene="aroC"
                     /locus_tag="VFK00_01985"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7591757.1"
                     /translation="MSSNTFGKLLSVTTFGESHGPAIGCVVDGCPPGIAIASEEFEHD
                     LARRATGRSRYTSQRHEADAVEILSGVYEGTTTGTPIALLIRNTDARSKDYDELARTF
                     RPGHADYTYWQKYGIRDPRGGGRASARETTMRVAAAVIAKKWLRERHGVEVQGWLAQM
                     DDIVPDTTGPIDFGVVESNAFFWPVAAQVRELEQRIDALRKAGDSCGARVGVVATGVP
                     PGWGEPVYGKLDAELAAALMSINAAKGVEIGAGFACVAQKGSVHRDEMGLDGFASNHA
                     GGILGGISSGQDIVASIALKPASSIPTPGRSVNMEGEEVDVRTKGRHDPCVGIRATPI
                     AEAMVALVLMDQALRHRAQCGDVGEVKPRIA"
     gene            complement(15849..16370)
                     /locus_tag="VFK00_01990"
     CDS             complement(15849..16370)
                     /locus_tag="VFK00_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7591758.1"
                     /translation="MEIVTARLRLDDLRPEDAEALFAYRGDPAVSRYQGWKPESVADA
                     ARFIEAQQGAAPDVPGTWWQRAIRLRDSGELIGDLGLHFFDDAAVELGISLAPAHQGR
                     GYAREAVEVMFDFTFGGLHKPRVIALVDPRNTPSIRLLEGLGMRRSGAEADSLVFTLP
                     AREWLATPDDEDG"
     gene            complement(16373..17287)
                     /gene="prmB"
                     /locus_tag="VFK00_01995"
     CDS             complement(16373..17287)
                     /gene="prmB"
                     /locus_tag="VFK00_01995"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7591759.1"
                     /translation="MHELLTIVDLIRFAASRFNAAGLTFGHSHDNAIDEASHLVLSSL
                     HLPPDLPPAYGGARVTAEERSRVLDLIDRRIDTREPVAYLVGEAWFAGMPFKSDRRAL
                     VPRSPIAELIEGGFSPWLDGQDVGRALDLCTGSGCIGIAMAAHQPNWRVDIADISDDA
                     LALARENIALHQLERRVRAVRSDLFASLRGECYDLIVSNPPYVTDAEYAALPGEYAHE
                     PKLGLAAGADGLDIALRILRDAPDHLTEGGLLVVEVGESERALAALLPEVPFVWIEFK
                     VGPMGVFALERHDLAAHADAIRAAADAR"
     gene            17387..18217
                     /gene="asd"
                     /locus_tag="VFK00_02000"
     CDS             17387..18217
                     /gene="asd"
                     /locus_tag="VFK00_02000"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460408.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HET7591760.1"
                     /translation="MRPSTLFQHIAPQKTLSRIVRAATRWTWRPWKDFLIGRVVRSYQ
                     VDLQEAEQPDPRAYPSFNAFFTRALLEGARRAEGDDATVVSPVDGRISQIGPVEGGRI
                     VQAKHHRYTVAELLADEVAAARYRDGSFATLYLSPRDYHRVHMPIAGELVETVYLPGR
                     LFSVAPAPVASIPRLFARNERLVCHFEGEQGPFVVAMVGAMLVSGISTVWNGPEVRAR
                     AIARRDWRGRGIRLARLAEMARFEMGSTVIVLLPPPACFDPAFIADAPVQLGQRLGHL
                     "
     gene            18281..>18600
                     /locus_tag="VFK00_02005"
     CDS             18281..>18600
                     /locus_tag="VFK00_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591761.1"
                     /translation="MSLVLEHPKSGGNYLRRLVTRVTAMGRNARLRRQLADIEQRCLE
                     LPRSYRDQLAELIGRECDNVARSPNPSLYGTHTEDGVEVGGLEVGLDRARSDNVQVRM
                     RGLAL"
BASE COUNT         2882 a         6477 c         6188 g         3053 t
ORIGIN      
        1 tgtccatcgc cacctccagc gagggcggac ggtagacggc ttgttcgtac cacttcatcc
       61 acacggaagg atcgatggtc tcgcggatgc ccccgccgag gatcctctcg tagggcttgc
      121 tgaagagttt gtagaggccg cccatgaaag acgcatagga gtcgttcggc acgtcgagtt
      181 ccgcgcctgc ggcgggcacg aacttctcgc tgaaagcgag cccggcaccg gcggccttgg
      241 cctgcatcca ggccaagggg atctccggca gcgtgtcggg gctgtgcccc gcaccgtcgg
      301 ttttctcgcc gccgccgacg tccgaatgcg cgccgatgaa ccagcgctgc tcgacttcga
      361 tctgctcggg cttccagacg gtgtgcgact cgcccggcgc cagggtgccc gccggccggg
      421 tccacttggt cggcttgaaa tcggcgcggt gctcgtcgag cgccaaggcc tggtacgcgt
      481 actggacgat cttgctgagg tcggtgtcgt ggaacgcgta gcggctgcgc gagaacggga
      541 accacgagcc gacgccgggg atgccgagcg caccgacggt gtcccacacg ccgatgaagc
      601 ggatcagcac ttcgcgcgaa tgggcggcgc ggaatgcctg catcgcggga tcattggggt
      661 gcaggttgtt ccggtacagc gcgtaggccg cgtccacgtc cgacctcggg acgtcgccct
      721 tgtcgtcggg attcagaagg ccgcacttgc ggatcaaccc gccgaggctg cgcgcggtgt
      781 aggcaccgcg actgaaaccg agcagccaga tctcgtcgcc ctcttgccag gtcttcgaca
      841 accattggta gccgtcctcg acattgcccg acaggccaag gccgaatgcg ccgcccagcc
      901 aatgttcgat cccggccttg acccccacgc ccgcgaaata cttgcagacc tgttcatgcc
      961 cggcgccatc gaccggcgcg accagcttcc agaaacgctc gacgttggtg agcgtctccg
     1021 ccttgctcca ggtgccgtcg aacaacgcga cgagtcggcg tggcatgggc aaattccccg
     1081 ttcgtcgtca tcccggcgca ggccgggatc catcatcatc gatgccaaac gcacggcccg
     1141 ggcagagcat ggcggacgat gccggccctg caccggtacg ctcgacgcga acggctgtcc
     1201 accgggtcgc tcgatcccgc tatgcgctgc gccgctgcgc cttggcttcg tcagaacgag
     1261 cgaactcgag ttgccgcagg tactcgggtt cgaccccggt gacgtaacaa cccgagaagc
     1321 acgaatcgtc gaagtgcttg agcttgtggt tgccttcggc gacggcctcg atcaggtcgg
     1381 ggaggtcctg gtacaccagc cagtccgcac cgatggtttc ccggatgtcc tcgaggctgc
     1441 ggccgtgcgc aaccagttcc gccggggccg gcatgtcgat gccgtacacg ttggggtagc
     1501 gcacgggcgg agcggccgag gcgaagtaca ccttgttggc gccggcgtcg cgcgccatct
     1561 gcacgatctg gcgggacgtc gtgccgcgca cgatcgagtc gtccaccagc agcacgttct
     1621 tgttgcggaa ttcgagcggg atgggattga gcttgcggcg aaccgagcgc acccgctggc
     1681 cctgccccgg catgatgaag gtgcggccga tgtagcggtt cttgacgaag ccttcccgga
     1741 acggcacgcc cagcgcggtg gcgagcgtcg agccggcggt gcgcgaggtg tcggggatcg
     1801 ggatcaccga atcgatgtcg tggtcggggc gctcgcgcag gatcttcgcg gcgagctttt
     1861 cgcccatccg caggcgcgcc ttgtacaccg acacgtcctc gatcatcgaa tcggggcgcg
     1921 cgaggtacac gtactcgaag atgcacggcg cgtgcaccgc gccctccgcg cagcgtcgcg
     1981 ccgacagcgt gccgtcgaag cccatgaaca cggcctcgcc cggtgccacg tcgcggacgc
     2041 ggcggaaccc gaggatgtcg aaggcgaccg attcggacgc acaggcgtat tccttgccct
     2101 cgggcgtgtc gcgttcgccc agcaccagcg ggcggatgcc gtgcggatcg cggaacgcca
     2161 gcacgccgta gccgaggatc atggcgacgc aggcgtagcc gcccttggcg cgcgcgtgca
     2221 ccgcggccac cgccttgaac agttcgtcgg gctccagcgc atggcggtcc tggatctgca
     2281 gctcgtgcgc gaggatgttg agcaggatct ccgaatcgga agcggtgttg atgtggcggc
     2341 ggtcgttctc gaacatctcg cgccgcaact gctcggtgtt gatgagattg ccgttgtggg
     2401 cgaacgcgat gccgtacggc gaattgacgt agaagggctg ggcctcctcg gaatcctccg
     2461 cacccgccgt cggataacgg caatgcccga tgcccgcgtg gcccctcagt tcctgcatgt
     2521 cgcgcgccga gaacacatcg cggaccagcc ccggaccccg acgcaggtgc agcttgctcc
     2581 cgtcgaaggt ggcgattccg gccgcgtcct ggccacgatg ctgcaatacc gtcaacgcgt
     2641 catacaacgc ggtggcgact tcggtcttgc cgacgatgcc gatgattccg cacatggaac
     2701 gctatgcctc gaagaacccg aatgtcgtgc cgcggccaag ccggaacacg tttccacgac
     2761 ccgcggtcga ccccggccag gcgggattcg acggcccctt tatccgcggg gctggtgcac
     2821 actcatcaga ccgattccgt ggacgctatt gtcccacatc acgccggtcc gggccgtgtc
     2881 ccggcgcacg tcccggcggg accgaggcag ccggtggccc ggcttgcttc tggagcgtcg
     2941 atatggggat ctgcgacagc gcgggcaagg cctggccgcc gatctcgaga tagtggctca
     3001 cctctgcggg aagccgctcg gcgaaccacc ccgctccgcc ctcgaatgcc ggcagcaacg
     3061 tggactggcg ccaccatgcg gcctgccgcg gcaccgacgt gaaaccgagc atcagcaccg
     3121 cgaacgtcac cagcagcact ccccgcgcga agccgaacac cagccccagc aagcggtcgc
     3181 cgccgcgcaa cccgccgtgg ctgagcagcc ttcgaaccat ccacccgagc agcgcacctg
     3241 caatcaggac accgaggaag caggtgagat agcctgccac gatgcaggcg gtcggctccc
     3301 gcaggaatcc gccgtagaac gcggcaacct cgtcgccgaa cgcccacgcc acccagaacg
     3361 ccgcgatcca gcaggcgagc gacaacactt cgctgatgaa gccgcgccac acgccgtaca
     3421 gcatcgagag agcgagcacc gccaggatca ggatgtcggc cgtattcatg ttctccgagc
     3481 catctactcg gtaactacat tgcccttgat cttgatctcg tcggcgatcc gcccgcgcgc
     3541 gctctcggcg acctcgcgcg tggcgaacgg acccgcgcgg acgcgccaca aggtgccctt
     3601 ggacgttttc acgctgtcga cgtagccgtc gaacccttgg ctgcgcaggc gatcgcgcag
     3661 gttctcggcc gaggattggt cgctgaacgc ggcgagctgc acggcccacc cgcccggcgt
     3721 cgaagccggc agcgccgagg ccggcgcatc gccctgcttg tcgacgctgg cggtgtcgac
     3781 gctcatcgcg acgtgctcga aatccttgag cttgagccgc gcggcttccg ccgcggcctt
     3841 gctggaaaac ggcccgaccc gcacccgcag tactgacttg ccccggaacg tctccggcgt
     3901 cgccaccgcg gtgaacccgt gccgcttcgc gtccgcgacc agcctgcggg cactgccgtg
     3961 gtccgcgtaa atcccgaggt tgacggtgta gaccgtcccg gcggcggcac ccgcaccgcc
     4021 agccactggc tccgccggct ccggcgccga ggatatgggc ttcggctcag ccgacgctac
     4081 cttggtcgaa gatgccgcgg ccgcgccggt ggcttccgtt tcggccgccg acgacgcggc
     4141 gagcggcgtc acggccgtcg ccccgggagc gaccgtagcc gtctggtggg tgccgccatt
     4201 caggtcaagc gaggcaacgc gatccggatc gacgatcgaa gcagcggtgc tcgaccccgc
     4261 gctggcgctg tccggaccga cctgcaagac gcgcgtctgc aactcgctgc tcggcggcgg
     4321 cggcacgtcg gtactcaggg gctcggttcc ggagcggctg ggaagcagca tcggcacgaa
     4381 gatcaccgcc agcgcgatca gcaccgccgc acccagcaga cgttttttca ggcctgaatc
     4441 catatatacg gcaccatgaa acggggcagt gtcagcgcag acacgaaaac ggaagcgttg
     4501 agccgcgcgc gaaaacccgg tatcgagcgg cgcgcctggt cgcgtcggct ccaacgcgcc
     4561 cagagcgagt ggtcggaatt atacggggca tgcccgcagc acgggcgcca caacgtggaa
     4621 gctgccgaac gcgacgatcc gcccatcggc ggaggcttcg gcacgcgccg ccgccagcgc
     4681 ggacgtgacg tccggatgtt cgctgcacga ggcagccggc gcggccgcgc ggaaacgcgc
     4741 cgccagtcgt tcgatcggct cgccgcgcgg cgactcgccg tcgaggcccg cgaggtacca
     4801 gtgatcgacc cgatcggcaa gcgccgtcgc cacggcttcg atgtccttgt cggccagcgc
     4861 gccgaacacc gcgcgcgtgg atcccggcac cgggtgacga tcgagccagc gcgcgagttc
     4921 ccgcgccgcc tgcggattgt ggccgacgtc gacgaccagg tcgggcgccg acgcgagatg
     4981 ctgcaacctg cccgccagga acggactggc cgacggggtg cgggcaaatg cgtccttctc
     5041 gatggccgcg gcactccagc gaacccgctc gcccagtgcg tgcagcgctg cgatcgcggc
     5101 cgccgcgttg cggtactgga acgcgccctc cagcgcgagt tcgccgtgca gggcgacgcc
     5161 gccggcgtgg ccttccgccg gtgcgacgta gcgcacctgc gcgggatccc gatgccacca
     5221 gaccagcgtt tcggtttcgg gcgaccaccc gaagtcgcgg ccgagcactt ccagccgcgc
     5281 gccgatcgcg tgggcgtggt cgagcaacga tgccggcggg ttcgtctcgc ccacgatcgc
     5341 gggtttgccg gcgcggaaaa tcccggcctt ctcacgcccg atcgcctcgc gatcggaccc
     5401 gaggtactcc atgtggtcga ggtcgacggt caccaccacc gcgcaatccg cgtcgacgat
     5461 gttgacggca tcgagccgac cgcccagccc gacctccagc agcgccacgt ccagctccgc
     5521 gcgcgcgaat accccgagtg ccgccagcgt gccgaactcg aaataggtca gggggatggt
     5581 cgcggatcca tcgccgatgc gcgccgcttc gacccgttcg aaggcttcga ccagcgaggc
     5641 atccctggct tcgacgccgt ccacccgcac ccgctcgttg tagcgcagta tgtgcggcga
     5701 cgtgtacgag ccgacccgca tcccgcccgc gcgcagcatc gcttccagga acgcgacgac
     5761 cgaacctttg ccgttggtac cgccgaccgt gaccaccagc ggcgccggcc gtggcgcgcc
     5821 catccggcgc catacctcgt ccacgcggtc gagacccatg tcgatgccgc gtgcatggat
     5881 cgtctgctgg taggccagcc attcgtcgag cgtcttcatg actcaccgcg cctcgcgcca
     5941 agggccgggc atgaattcat tcgcccttcc gcggcaagca caatcgatgg actgcgcccg
     6001 ccctccccct tccgccgaaa gggagatcga ggggatggcc tttggtgtca cgccaccttg
     6061 tcgcggtgca ccaagatcgc gagcaaggcc gcgatccggt cgcggatttc gcggcggtcg
     6121 cagatcatgt caatcgcgcc gtgctcgagc aggaactccg aacgctggaa accttcgggc
     6181 agcttttcgc gtacggtctg ttcgatcacg cgcggtccgg cgaaaccgat cagcgcacgc
     6241 ggttcggcga tctggatgtc gcccagcatc gcgaggctcg cggaaacgcc gccggtggtc
     6301 ggatgcgtca gcaccgcgat gtacggcagg ccggcatcgc gcaggcgggc gagcgcggcg
     6361 gacgtcttgg ccatctgcag cagcgactgc aggccctcct gcatgcgcgc gccaccggtc
     6421 gccgtaaagc agacgaacgg cgtcttgttg cgcaacgcgg cctcggcggc gcgggtgaac
     6481 ttctcgccga ccaccgaacc catcgagccg cccatgaagg agaactcgaa tgcgcaggcg
     6541 atggccggca ggcccttcag cgcgccctgc accgcgatca acgcgtcctt ctcacctgta
     6601 gccttctgcg cggcgaggat gcggtcgcga tatttcttgg aatccttgaa cttgagcgaa
     6661 tcgacgggac ccatgcccgc gtcgagctca ttgccgccct cgtcgaggaa tgcggccagc
     6721 cgcgcgcgcg cgccgatcgg atggtggtat ccgcacttcg ggcacaccat caggttgcgc
     6781 tcgagctcgg ggcggtacag cccgatgccg cagcccgagc acttctccca cacgccttcc
     6841 ggcaccttgc ccttcacggg cgcgccctcg gtgcggatgt tcggcgacgt cagcttctgc
     6901 agccaggaca cggggcggct cctgccgttt gccagtcgcg taggatacac cggcggccaa
     6961 tgcggccgta agccgcggat cgtcggcccg gcggggccgg ggcccgtcac gcccgggtgc
     7021 cgtcgagggc gctccgtatc ggcgcgagga aatcccgcgc acgccggcag gcatcgccgg
     7081 catcgttcgc gtgggcgagc gagccgacca gcgccgagcc gatcaccacc gcatcggcga
     7141 acgctgccac tgccgcggcg ctggaggcat ccttgatccc gaacccgacg gcgaccggga
     7201 ccttcgcgcg ctcgcgaatc gcgccgaccc gctgcgcgat gtccccggtc gacaatttcg
     7261 cggcgccggt gacgccggcg aacgacacgt agtacaggta accttcggcc gcctcgatcg
     7321 cgcgcgcgag ccgcgcgtcg gaggtcgtgg gcgccgccag caggatgcgc tgcaggccgg
     7381 catcgcgcag cggttgcagg gtggcgtctt cctcgatcgg gcaatcgacg accagcacgc
     7441 catccacgcc ggcctccacg gcctcgcgcg caaagcgttc gattccgcgg atcgcgatcg
     7501 ggttgagata gcccatcaac acgagcggcg tttccgcgtc gcgctcgcgg aattcgcgcg
     7561 cccagcgcag gatgccaccg acgccgacct tgttgcgcaa ggcccgctcg tcggcgtgct
     7621 ggatcaccgg gccatcggcc atgggatcgg aaaagggcat cccgagttcg atcaggtcgg
     7681 cgccggcctc gaccagcgcg tgcatgacct cgacggtcgc ccctggaaac gggtcgccgg
     7741 cggtgatgaa ggggatcaac gccgcgcggc tgcgctgctt cagcgccgcg aagaccgccg
     7801 ctatgcggtc ctcccccgtt ccccgttccc cgttcccgct gtcgctggcc gtgttcacag
     7861 ctcgaacccc tcgcgctccg caatcgtatg cacatccttg tcgccgcggc cggaaaggtt
     7921 gcacaggatc agcttgtcct tcggcagccc gcgcgcgagc ttgatcgcct gtgcgacggc
     7981 gtggctcgat tcgagcgctg cgaggatgcc ctcggtttcg cacagcaggt ggaaggccgc
     8041 gagcgcttcc ttgtcggtga cgccgacgta ttcggcgcgg ccggtatcct taaggaacgc
     8101 gtgttcggga ccgacgccgg ggtagtccag gcccgccgac acggaatggg tttccgtgat
     8161 ctggccgtca tcgtcgcaca acacgtaggt ccggttgccg tgcagcaccc ccggtcgccc
     8221 ggccgcgagc gacgccgcgt gatggccgct cgcgatgccc tcgcccgccg cttcggcgcc
     8281 gatgatgcgc acgtcgcgat cgttgaggaa cgcgtggaac aagccgatgg cattggagcc
     8341 gccgcccacg cacgcggtga ggacatcggg cagtcgcccg aattgctcga gcatctgcgc
     8401 gcgcgcctcg cgtcccgcca ccgcgttgaa atcgcgcacc atcatcgggt agggatgcgg
     8461 ccccgcgacg gtgccgatga tgtagaaggt gtcggcgaca ttggtcaccc agtcgcgcag
     8521 ggcttcgttc aacgcatcct taagggtttt cgagccggat gtgaccggaa ccacctcggc
     8581 gcccagcagt ttcatgcggt acacgttgat cttctggcgc tcgatgtcga ccgcgcccat
     8641 gtacaccacg cacttcatgc cgaagcgcgc gcagaccgtg gcggtggcga cgccgtgctg
     8701 gccggcgccg gtctcggcga tgatgcgctg cttgcccatg gccctcgcga ccagcgcctg
     8761 gccgacggtg ttgttgatct tgtgcgcccc ggtatggttc aggtcctcgc gcttcagcag
     8821 gatctgtgcg ccaccgacgc gttcggacaa gcgcttcgcg tggtagatcg gcgagggacg
     8881 tcccacgtat tgccgcaggt catcggcgaa ttcattctgg aacgccggat cgtcgcgcag
     8941 acgcgtgtat gccgcggtca attcccccag cggcgcgatc agcgtctcgg cgacgtaggt
     9001 gccgccgtac ggaccgaagc ggccattcgc gtctggatac ctggagtagt cgatcgcttc
     9061 tttcatgcat gaaccttctt gccacggatg aataccgatc tccgcggagc acccctgcca
     9121 atcttttgtg cttcgatccg ggttgatcgt tgtcagagcg gtgcttctgc ctccgccacg
     9181 gcagcaaaaa acgcgcggag cttgccggcg tccttgatgc cgggcgccga ttcgacgccg
     9241 ctggcgagat cgaccgccca cggatgcacc gcgccgatcg catcggcgac attgcctgcg
     9301 ttcaatccgc ccgcgaggat aatcggtcgc cccagctcct gcggcacgcg cgaccagtcg
     9361 aaggccctgc cctgtccgcc ctgctggccc ggcggatggc cgtccaacac gaaaccggct
     9421 gcccgtgcgt gctcggcggc aaccgcgcgc acgtcgaggt cgcccgccat gccgagggcc
     9481 ttgaggtagc gtagcccgaa cgattcgcag aatgccgcgc cttccgtgcc gtggaactgc
     9541 acgaggtccg gctgcacgcc gtcgatgatc tggcgcacgc gatcggcggg caggtcgtgc
     9601 gacaacacca ccgcgtccac gaacgcgggc agtgcgtcgc ggattgcgct cgcacgctcg
     9661 atcgacacgc agcgagggct gctcggggtc atcaccatgc cgatcgcgtc cgcgcccagc
     9721 tcgcaggcga gcacggcgtc ctcgacgcgg gtgactccgc agattttcac gcgtatacgg
     9781 gacattacgg gatgggtcgc gggcggcgcg ccctacaggg tcacttcggg tggaagcgac
     9841 cattctgacg gatatttcgg gccgaggaac gtcaatccct gtggcggggc ggtgacgccg
     9901 gcgacgttgc ggtcccgcgc cgccagcagt tcggccatcc atccggccgg gcgctcgccc
     9961 cgaccaactt ccagcagcga gcccacgatg ttgcgcacca tgtggtgcag gaaggcgttg
    10021 gcctcgatct cgatgatgac gcgcccgcca tcgcgccgca cgctgacttc ggtgacgttg
    10081 cggcgcgcat ggcgcgcctg gcacgacacc gcgcgaaacg aggaaaaatc ccgctcgccg
    10141 accagggcct gcgccgcggc atgcatcgcc ccggcatcga ggggcacccg ttcccacgac
    10201 aggaatcgcg catcgagcgc gggccggatc gcgcggttga ggatcgcgta gcggtagcgc
    10261 cgcgcacgcg cggaaaagcg cgcgtggaaa tcgtcgggca ccgcacgcgc ccacaacacc
    10321 gcgacctcgc gcggcaagcg ggaggtcgca cccaatatcc acgcacgctc gctccgggcc
    10381 gcgtgggtgt cgaaatgcac cacctggcag cgcgcatgga cgccggcatc cgtgcggccc
    10441 gcgcaggtaa cctccacagg atgatcggcg acgaacgaca atgccgattc cacctcggcc
    10501 tgcacgctcg gtccgtgacc caaccgctgc cagccgaaga aatcggtgcc gtcgtattcg
    10561 atgcccagcg cgacgcgcat cgagccagcc ggtccgccct cgagacctgc gtcggtcaac
    10621 tcaatccgtg ctctcggcga gcagtcgccg cgcttcgtcc ttttgcgtct gcgagccttc
    10681 ttcgagcacc tcgtcgagca tcgcgcgcgc gccgtccgga tcgcccatgt cgaggtaggc
    10741 acgcgcgaga tcgagcttgg tgtcgacggg atcgtcgctg aactggacgg cggcagtttc
    10801 ggcggacgcg ggttccggat gcgcctccgt gatcgtgccc attaccggaa gcgactcgtc
    10861 ccaagcatcc ggtgccggtt ccggtcggct ctcgggggcc ggttcgtcct cgaccgcctc
    10921 gctcaccggt tccatctcca gatcctcgcc gggcgcgaaa tcctgcggtg gcacgaccac
    10981 ctccggctct tcctcgacct ccgtttcgac aaccgccgtt tcggccgggc cttccgccgg
    11041 ggcggcgaac agcggatcgc cgggcgccag ttcctcgccc atcacggcga cctcgtgcca
    11101 ttcgaggctg ccgggttccc ggacgtgcgc atgcatcgct tcggctgccg cgacgaaacg
    11161 cgcggcatcg ccctcggcgt ggtacagacg gcagagcgcg aggtgggcac ccagatcgtc
    11221 ggggttttcg gcgagttgcg cttcgagcga ggcccggtag gcggcggtgc tggccggggg
    11281 cgcaaggtcc tcgggatcgg tcaatggccc gggcacgccg gcctccgctt ccgtgccacc
    11341 gcggcggcgc gccagtagtc cgaccaggat cagggcgagc gcgatcaccg ctgcgatgat
    11401 ccatgtcagc ggccgttcga accagggcgt tgccgatgcc ggcgatgtgg ctgctgccgt
    11461 cttgccggcg ggcttggcga ccgcggtttt ccccgattca ttgccggcgg cggcggtcgc
    11521 aactcccgcg gacgatgtcg ccgcgagggg tttccccgcc tgcacggccg ccagtttccg
    11581 ctggagttcc gcaatcgtgg cgtccttgaa actcaataac ttggcggact ttccggcaag
    11641 atcttcgaga ctgtgcatcc gcgaatcgag gtcggcgttc gactgctcca ggctggccag
    11701 ggtgccgcgc tcgttctgca gccgttgctg cagacccgcg accgtttcgc tgcctgtgcc
    11761 gccagcgacg ccgggccggt tgccggcgcc gctcgcgccg ccgctcggcg gcaccagcga
    11821 caaatggtcc gtttccgggg ccgcgaccgt cggtttcggt gccgtcctgg cggcagcatt
    11881 cgcggcagtg ccttccagga cggtcgccgg atgtggccgc gcggagcgcc acgcctcgta
    11941 ctgccgatgc actgccgcgt cggcctcggc gatgctggtc tggtcgattt gctcacgggt
    12001 cgggatgcgc aggatcgcac cggccttcaa atcgttgatg ttgtccttga agaacgcttc
    12061 gggattcgcg gacttcagcg ccatcatcat ctggttgacg gtggtgccgg tgtcaggggt
    12121 gacccgcttg gcgatgccat acagggtgtc gccgcgctcc accgggatcg tgccggacgc
    12181 cggggccgca gccgcgggtt gcggcgcagg ctggggcttg ggcggagccg gcggtggcgt
    12241 cggctttcgt gccgccgcct ggggctgtgg ttgcggcttg gccggcggtg gtgctgcctg
    12301 ctgttgttcc ggtttcgctg gtgccggggc ctgcggcggc ggcgcgggct gcgcggctgc
    12361 ttcgggcggc ggcaactgcg ccggatggga cggcggcggc gcggcgaccg gcgacgaaat
    12421 gggcgccgtc tgcacggccg gcgcgggcgc cgagatcacc gggttcagca ggaccacgaa
    12481 ctcgcgcacc tgcttggcct ggcgcgtgtt cacttcgatc aggaaatcga gataggggtc
    12541 gctgaccggc gcgctgctgg tcaccaggat cagcttctgg ccgttgttgt cggtcaccac
    12601 gtggaaacgc agcgtccggt cggtcgcggt caagggaagg ccggcgcgcg cgaagtcgtc
    12661 ggccgacgcc agcttgaccg tcaatccgtc cagctcggag aggttcttcg gctgcagcgg
    12721 aatcgccgcg accagcggct ggttcaacgc ggacttgacc tcgatctgcc ccaattggag
    12781 cgcccaggcc ggggacatgg ccaaggcgag cagtgcggca cctgcgatgc ggagtttcaa
    12841 cttcatggga cccaaccctg ctgtcgaccg gggcgcgctg gactccgcct ccgtgcgcgc
    12901 gcgccccgcg caagggtatt gcagaaaccg gcaatcacca agccggcgga acccgccgaa
    12961 acctttgccc gtcagtgacc cgaggtcgcc ggtgacggcg cggcgcctgc cgcctcgagc
    13021 ttccgctgca aggccgcgat ttcctggtca cgttgctgca attgcttctg gcgctcggca
    13081 ttttgctgtt ccatcttcgc cacctgcgcc tgcagctcgg cgttgcggtc ctgcgccttc
    13141 gaaaccttcg cctgcagatc gtgcaactgc gcctgcacgg ccttgtcgcc gtggctttcc
    13201 gcagccacgg cggacggccc cgcgagcaac atgccgatgc ccatgagcac gcacagcata
    13261 cccgtccgca tcaaccgctt tggcaacctc atggcccacc tcgtcatgat tcttttaacg
    13321 tcgtcattcc ggatctcccc cacttcgtgg gctcgtacga aacgacgaat gcatttccgg
    13381 gacagcacaa caccgtgcaa cgcttctctc cgcaccctgt tccccgttcc ctgttccctg
    13441 ctctaaaggt actcgcgaat caggatttcc gcgacctgca ccgcgttgag cgccgctccc
    13501 ttgcgaatgt tgtcggacac cacccacaaa tccagccccg tggggtgcga gatgtcgtcg
    13561 cggatgcggc ccacgaacac ggcgtccttg cccgcggccg cgccgaccgg cgtcggatag
    13621 ccgccgggct tgcgctcgtc caccaccacc acccccggtg cggcttccag cagcgcgcgg
    13681 gcggtggccg catcgatctt ctcgcgcgtc tcgatgtgca cggcttcgga atggccgaag
    13741 aacaccggga cgcgcacgca ggtcggattc acctggatcg catcgtcctc gaggatcttg
    13801 cgggtctccc agaccatctt catctcttcg cgggtgtagc cgttgtcctg gaacgcatcg
    13861 atgtgcggga tcacgttgaa ggcgatctgg gcgctgaatt tttccggctc gatttcccgg
    13921 aaattgagca ggtccgccgt ctggcgcccg agctcctcca ttcccgaacg cccggcgccg
    13981 gacacggact ggtaggtcgc cacgttgatc cgctcgatgc cggcggcgcg atggatcggc
    14041 gccaacgcca ccagcatctg catcgtcgaa cagttgggat tcgcgatgat gttgcgattg
    14101 gtgtattcgg cgatggcgtg cggattgact tcgctcacca ccagcggcac ggaatcgacg
    14161 taacggaact cggaggtatt gtcgatcacc acggcgcccg cgtccgccgc gcgcggcgcg
    14221 tgttcccggc tgacgcctgc accggccgag aagaacgcta tgtcgatgcc ggaaaagtcg
    14281 tagctcgcga cctcgcgcac ggtcacctgc ttgccgcgga atttcagctt gctgccagcc
    14341 gagcgctcgc tggccagcgg aaccagttcg gccacgggga atgcccgatc ctcgaggatc
    14401 gacaacaggg tttcgccgac cgcaccggtg gcgccgacca tggcgaggtt gtattgggtt
    14461 tttttggcca ttcgttgcag ggctcgtcga tatcgggact cgggactcgg gactcgggac
    14521 tcgggactcg ggacagcata atcagaacgt cgtcattccg gggccgttgc cagcttttcg
    14581 gcaacggacc ccggaatctg ctcttcggtt actcgctcgg cgcagaagcg gattccgggt
    14641 tcggcctgcg gccgccccgg aatcacgaag aaatggacgc tcgcggaaat cgaaaaacaa
    14701 cgtaccaaca acatccaggt caggcaatcc gcggtttcac ctcgcccacg tcgccgcact
    14761 gggcgcggtg gcggagtgcc tggtccatca gtaccagcgc caccattgcc tcggcgattg
    14821 gcgtcgcgcg gatgccgacg cagggatcgt ggcggccctt ggtgcgaacg tccacctcct
    14881 caccctccat gttcacgctg cgccccggcg tggggatgct cgacgcgggc ttaagcgcaa
    14941 tcgaagccac gatgtcctgg ccgctgctga tgccgccgag gattccgccc gcgtgattgg
    15001 acgcaaagcc gtccaggccc atttcatcac ggtgtacgct gcccttctgc gcgacgcagg
    15061 cgaagccggc gccgatctcg acgcccttgg ccgcgttgat cgacatcagc gcagcggcga
    15121 gttccgcgtc gagcttgccg tacaccggct cgccccaccc gggcggcacg ccggtggcga
    15181 ccacgcccac ccgcgcgccg caggaatcgc ccgccttgcg tagcgcatcg atgcgctgtt
    15241 ccagctcgcg cacctgcgcc gcgaccggcc agaagaacgc attggattcg accacgccga
    15301 agtcgatcgg ccccgtcgtg tcggggacga tgtcgtccat ctgcgcaagc cagccctgca
    15361 cttcgacgcc gtgcctttcg cgcaaccact tcttcgcgat caccgcggcc gcgacccgca
    15421 tcgtcgtctc gcgtgccgac gcgcgtccgc cgccgcgcgg atcgcggatc ccgtatttct
    15481 gccagtaggt gtagtccgca tggccggggc ggaaggtccg cgcgagctcg tcgtagtctt
    15541 tcgaacgcgc gtcggtgttg cggatcagca gcgcgatcgg cgtaccggtg gtcgtcccct
    15601 cgtacacccc ggaaaggatc tccaccgcgt cggcctcgtg gcgttgcgag gtgtaacggg
    15661 acctgccggt ggcccggcgc gcgaggtcgt gctcgaactc ttccgaagca atcgcaatcc
    15721 ccggcggaca gccgtcgacc acgcagccga tcgcgggccc gtgggattcg ccgaaggtcg
    15781 tgaccgagag gagtttgccg aaggtgttgg aggacacggc gcgcgatgcg agcgggaacc
    15841 gcgcaagctt acccgtcttc gtcgtccggc gttgccagcc attcgcgtgc cggcagcgtg
    15901 aacaccagcg aatccgcctc ggcgccactg cgccgcatgc ccagtccttc gagcaggcgg
    15961 atgctggggg tgttgcgcgg atcgaccagc gcgatcacgc gcggcttgtg cagcccgccg
    16021 aaggtgaagt cgaacatgac ctccacggct tcgcgggcgt aaccccggcc ctggtgcgcg
    16081 ggcgcgaggc tgatgccgag ctcgacggcc gcgtcgtcaa aaaaatggag gccgagatcg
    16141 ccgatcagtt cgccggaatc gcgcaggcgg atcgcccgct gccaccacgt gccgggaacg
    16201 tcaggcgcgg ccccctgttg cgcctcgatg aagcgcgcgg catcggccac cgattcgggc
    16261 ttccagccct ggtagcggct gaccgcgggg tcgccgcgat aggcgaacaa tgcttcggca
    16321 tcctccgggc gcaagtcatc cagccgcagg cgggccgtga cgatttccat gctcagcgtg
    16381 catccgccgc ggcccggatc gcgtccgcgt gcgccgccag gtcgtggcgc tccagcgcga
    16441 acacccccat cggcccgacc ttgaactcga tccagacgaa cggcacctcc ggcagcaacg
    16501 ccgccagcgc gcgttcgctc tcgccgactt cgacgaccag caggccgcct tcggtcaggt
    16561 ggtcgggcgc gtcgcgcagg atgcgcagcg cgatgtcgag gccgtcggca cctgcggcca
    16621 atcccagctt cggctcgtgc gcgtattcgc ctggcaaagc cgcgtactcg gcatcggtga
    16681 cgtacggcgg attcgacaca atcaggtcgt agcactcgcc ccgcaggctg gcgaagaggt
    16741 cggaccgcac ggcgcgcacg cgccgctcga gctggtgcag cgcgatgttc tcgcgtgcca
    16801 gtgccagcgc atcgtcgctg atgtcggcga tgtccacccg ccagttcggc tggtgcgccg
    16861 ccatcgcgat gccgatgcaa ccggagcccg tgcaaaggtc gagcgcgcgg ccgacgtcct
    16921 ggccgtccag ccagggcgaa aaaccgccct cgatcagctc ggcgatcggg gagcgcggca
    16981 ccagtgcgcg ccggtccgac ttgaacggca tgccggcgaa ccatgcctcg ccgaccaggt
    17041 aggcgaccgg ctcgcgcgta tcgatgcggc ggtcgatcag gtccagcacc cgcgatcttt
    17101 cctcggcggt gacccgcgcg ccgccgtagg ccggcggcag gtcgggcggc aggtgcaggc
    17161 tcgaaagcac caggtgcgaa gcctcgtcga tggcgttgtc gtggctgtgg ccgaaggtga
    17221 gcccggccgc gttgaagcgg ctggcggcga agcggatcag gtcgacgatg gtgagcagtt
    17281 cgtgcatgat gcgcgggcgc cggtggatgg cgcagtatag gcgaccgctc ttggcgcgga
    17341 ggcgactttc cgagtcccgg gtccccgctc catttagact gggccgatgc gcccttccac
    17401 gctcttccag cacatcgcgc cgcaaaagac gttgagccgc atcgtgcgtg ccgcgacccg
    17461 ctggacctgg cgtccctgga aggatttcct gatcggtcgc gtggtgcgtt cctaccaggt
    17521 ggaccttcag gaagccgagc agcccgaccc ccgcgcctat ccttctttca atgcattctt
    17581 cacgcgcgcc ctgctggaag gcgcacggcg cgcggagggc gacgacgcca ccgtcgtcag
    17641 cccggtcgac ggccggatca gccagatcgg gcctgtcgaa ggcggccgca tcgtgcaggc
    17701 caagcaccac cgttacacgg tggccgagct gctggccgac gaggttgccg cggcgcgcta
    17761 ccgggacgga agcttcgcga ccctgtacct ctcgccgcgc gattaccacc gcgtgcacat
    17821 gccgatcgcg ggcgaactgg tcgaaacggt ttacctgccc ggccgcctgt tcagcgtcgc
    17881 gccggcgccg gtcgcatcca ttccgcgcct gttcgcgcgc aacgagcggc tggtatgcca
    17941 cttcgagggc gagcagggac ctttcgtcgt ggccatggtc ggcgccatgc tggtctccgg
    18001 tatttccact gtctggaacg ggcccgaggt gcgtgcacgc gcgatcgcgc gacgagactg
    18061 gcgcgggcgc ggaatacggc tggcacgatt ggcggaaatg gcgcgcttcg agatgggttc
    18121 cacggtgatc gtgttgctgc cgcccccggc atgcttcgat cccgccttca tcgccgacgc
    18181 cccggttcag ctcgggcagc ggttggggca tttgtaggcg acctgcgttc acggcacaat
    18241 cgcaggacac gggggaaaat ggtgggtcac cgtgaggagc gtgtcgttgg tccttgagca
    18301 tccgaaatcg gggggcaatt acctccggcg tctcgtcacg cgcgtcaccg ccatgggccg
    18361 gaacgcgcgc ttgcggcgcc agctcgctga catcgaacag cgatgcctcg agctcccgcg
    18421 ttcctatcgc gaccagctcg ccgagctgat cggccgcgaa tgcgacaacg tcgcgcgaag
    18481 ccccaaccct tcgctgtacg ggacgcatac cgaagacggc gtcgaggtcg gcggcctcga
    18541 ggtgggactc gaccgcgccc gctccgacaa cgtgcaggtg cgcatgcgcg gcctggcgct
//
LOCUS       DASSNH010000055         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_581317, whole genome shotgun sequence.
ACCESSION   DASSNH010000055 DASSNH010000000
VERSION     DASSNH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581317"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /gene="metB"
                     /locus_tag="VFK00_02010"
     CDS             complement(<1..1181)
                     /gene="metB"
                     /locus_tag="VFK00_02010"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7591762.1"
                     /translation="MNNIASCTRAVRAGIETDGQHGAVVPPLHLSTNFSFAGFNAKRA
                     YDYSRSGNPTRDLLADALADLERGAGAVVTASGMAAVALVLELVPAGGKVLAAHDCYG
                     GTWRLLDAWAKKGRFEVCFADLTDPSALAAGLADRPALVWVETPSNPLLRITDIRHVA
                     QAAHAADALCVVDNTFLSPALQQPLTLGADVVVHSTTKYINGHSDVVGGAVIAREPSV
                     AETLKWWGNCLGLTGAPFDSWLTLRGLRTLSARLRAHQENAAALADLLNQHPAVTRVF
                     YPGLASHPQHALAQRQQSGFGAMLSFELDGGEGAVRAFLDGLQCITLAESLGGVESLI
                     AHPATMTHAAMALEARRAAGISDALLRLSVGIEDIADLSHHLRAALARADAVSRKRVA
                     VP"
     gene            complement(1183..2217)
                     /locus_tag="VFK00_02015"
     CDS             complement(1183..2217)
                     /locus_tag="VFK00_02015"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET7591763.1"
                     /translation="MSLAVAPETVAPCAPPVSRIPRVGLPFACQRGTRAIRVTPRYLG
                     HAVTTEVRYLWTGAPAAPTVIVQGGISADRDVCATSERAAPGWWEALVGRGRAIDLDR
                     VRVLAIDWLDADDLQVPSVSSEDQADALAALLDALGIARAQACVGASYGAMVGLAFAA
                     RHGDRLGELVALAGAHRPHPYATAQRAVQRGIVRLGLDKGCVDDALALARQLALTTYR
                     SSGEIGRRFAGGAEYRGGRFRLPVEDWLEHHGRKFVARCDANRYLALSESIDLHDIDP
                     KAVRVPATLIGFASDRLVPLADLCELQQRLGAPASLEVIETPYGHDGFLKEHERLAPL
                     LREALHDCGA"
     regulatory      complement(2304..2422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2623..3168
                     /locus_tag="VFK00_02020"
     CDS             2623..3168
                     /locus_tag="VFK00_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7591764.1"
                     /translation="MASDLFLRETARCLCLAARRASRAITREFDQALRRHGLRATQFT
                     LLAALHLAGPKAIGELAELLSADRTTLTRNLALAEQHGWVTLQADREDARSRIASVTP
                     KGSRVLKAALPAWRETQQRLADLMGAQAASSLRKIAGGPCTMEVSSSQSARGRRKAVS
                     KRQPAARHQDQHPAKHRGDRR"
     gene            3165..>3506
                     /locus_tag="VFK00_02025"
     CDS             3165..>3506
                     /locus_tag="VFK00_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7591765.1"
                     /translation="MKLYWFDTVNPRKVCAVAKYLQSPVEYVHVDLGRGEHKTPAYLA
                     LNPNGKVPTLVDGRRTLWESAAIMCHLAARSDSELWPQDGRQIEVIRWLSWDLAHFYK
                     WGGTLYFEHVIK"
BASE COUNT          610 a         1215 c         1132 g          549 t
ORIGIN      
        1 ggtaccgcca cgcgttttcg cgacaccgca tccgcacgcg ccagcgcggc gcgcaagtgg
       61 tgcgacagat cggcgatgtc ctcgatgccg acggacaacc gcagcagggc gtctgaaata
      121 cccgctgcac ggcgcgcttc gagcgccatc gccgcatgcg tcatcgtggc cggatgcgcg
      181 atcaggcttt ccacgccgcc cagcgattcg gccagcgtga tgcattgcag gccgtcgagg
      241 aacgcgcgca ccgcaccctc accgccatcg agttcgaagc tcagcattgc accgaatccg
      301 gattgctggc gctgcgccag tgcatgctgc ggatgcgagg ccaagccagg atagaacacc
      361 cgcgtcaccg ccggatgctg gttcaacagg tcggcaagcg cggcggcatt ttcctgatgc
      421 gcgcgcagcc gcgccgacaa cgttcgcagt ccacgcaggg tcagccagct gtcgaacggt
      481 gcgccggtga ggccgaggca attgccccac cacttcaatg tttcggcgac gctcggttcg
      541 cgggcgatca ccgccccgcc caccacgtcg ctgtggccgt tgatgtactt ggtggtcgag
      601 tgcaccacca cgtcggcgcc caacgtcaac ggttgctgca acgccggcga caggaacgtg
      661 ttgtcgacca cgcacaatgc gtccgccgcg tgcgcggcct gcgccacgtg gcggatgtcg
      721 gtgatgcgca gcagcggatt ggatggcgtc tcgacccaca ccagcgccgg cctgtccgcg
      781 agaccggcgg cgagcgcgga cggatcggtg agatcggcga aacacacctc gaaacggccc
      841 ttcttcgccc acgcgtccag cagccgccac gtgccgccgt agcaatcgtg cgcggccagc
      901 accttgccgc cggccggcac cagttccagg accagcgcga ccgccgccat gccgctggcc
      961 gtcaccaccg cccccgcgcc gcgttcgaga tcggccaatg catcggccag caggtcgcgg
     1021 gtgggattgc cgctgcgcga atagtcgtat gcgcgctttg cgttgaagcc cgcgaatgaa
     1081 aagttggtcg aaagatgcag cggcggcacc accgcgccgt gctggccgtc ggtttcgatg
     1141 ccggcgcgca ccgcgcgcgt acaggatgcg atgttgttca tgtcatgctc cgcaatcgtg
     1201 cagggcttcg cgcaacagag gcgcgaggcg ttcgtgctcc ttcaggaagc cgtcgtggcc
     1261 gtagggcgtt tcgatcactt cgaggctggc cggcgcgccg aggcgttgct gcagttcgca
     1321 caggtccgcg agcggtacca gccggtcgct ggcgaaaccg atcaacgtgg ccggcacgcg
     1381 caccgccttc ggatcgatgt cgtgcaggtc gatggattcg gataacgcga gataccggtt
     1441 cgcgtcgcag cgcgccacga acttgcgtcc atggtgttcc agccagtcct cgaccggcag
     1501 acgaaaacgt ccgccacggt attccgcacc accggcgaag cggcggccga tttccccgct
     1561 gctgcggtag gtggtcaatg caagctggcg cgccagcgcc aaggcgtcat ccacgcaacc
     1621 cttgtcgagt ccgagcctga cgatgccgcg ctgtaccgcg cgctgcgccg tcgcatacgg
     1681 atgcgggcga tgcgcgccgg ccaacgccac cagctcgccg agccgatcgc cgtgacgcgc
     1741 cgcgaaggcg aggcccacca tggcaccgta cgacgcaccc acgcaagcct gggcacgtgc
     1801 gatgcccagc gcgtcaagca atgccgcgag cgcgtcggcc tggtcttcgc tggacaccga
     1861 cggcacttgc aggtcgtccg catccagcca gtcgattgcc agcacgcgca cgcggtcgag
     1921 gtcgatcgca cggccgcgcc cgaccagcgc ctcccaccaa ccgggtgcgg cgcgttcgga
     1981 agtcgcgcag acatcgcgat cggccgagat gccgccctgg acgatgacgg tgggcgcagc
     2041 cggcgcgccc gtccacaagt aacgcacctc ggttgtgacc gcgtgcccga gatagcgtgg
     2101 cgtgacgcgg atcgcgcgcg tgccgcgctg gcaggcaaac ggcaatccga cccgcgggat
     2161 gcgggacacc ggcggcgcgc agggcgcgac ggtttcgggt gcgacggcaa gactcatcac
     2221 ggttctcctt tcaaaaccac gacatcgggt catgcggaga accggaaaac ggccgtgcaa
     2281 tcccgcgcac ggcgggactt cgcggctcat ctctcggaca gcgcatggcg cctccgcagg
     2341 agttggcacc gtgcggaccc ttgcggttcc gcgggttgcc ccggcttcca agggcctgtc
     2401 cctcagccgg tctcgatgag caagggacgc aatctagcgg catcgaattg ccgtgtcaat
     2461 agacgtatag acgtccatat cgatgattgc actttgcagc cagcccagcc catggcagcc
     2521 gtgattcggc tcgacggcgc cgcgcgggga catgaccatt gcctgcgctc gtcgttgcac
     2581 ggcgcttgac agtcgtctta tgtgcatata cacgtgtttg gcatggcgtc agacctgttc
     2641 ctgcgcgaaa ccgcccgttg cctgtgcctt gcggcgcgac gcgcttcacg cgcgatcacc
     2701 cgcgaattcg accaggcact gcgcaggcac ggattgcgcg ctacccagtt cacgctgctg
     2761 gcggcgctgc acctcgccgg cccgaaagca atcggggaac tggccgaatt gctgtcggcc
     2821 gatcgcacca ccctcacccg caacctcgcc ctcgccgagc agcacggctg ggtcacgctg
     2881 caagccgacc gcgaagacgc gcgctcacgc atcgcgtcgg tgacgccgaa gggttcacgc
     2941 gtcttgaagg ccgcgctgcc cgcgtggcgc gagacacaac agcggctcgc cgatttgatg
     3001 ggcgcgcagg ccgcctccag cctgcgcaaa atcgccggtg gaccgtgcac gatggaggtt
     3061 tcttcatcgc aatccgcacg tggccggcgc aaggccgtgt cgaaacggca gcccgccgcg
     3121 cgtcatcagg atcaacatcc cgcgaaacac cgaggagacc gccgatgaaa ctctactggt
     3181 tcgatactgt caatccgcgc aaggtgtgcg cggtcgcgaa gtacctgcaa tcaccggtcg
     3241 aatacgtgca cgtcgacctc ggccgtggcg agcacaagac acctgcatat ctcgccctga
     3301 atcccaacgg caaagtcccg acgctggtcg acggcagacg cacgctgtgg gaatcggccg
     3361 cgatcatgtg ccacctggcc gcccgctcgg attccgagct gtggccgcag gacggtcgcc
     3421 agatcgaggt gatccgctgg ctcagttggg acctggcgca cttctacaaa tggggcggca
     3481 cgctctattt cgaacatgtc atcaag
//
LOCUS       DASSNH010000056         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_816620, whole genome shotgun sequence.
ACCESSION   DASSNH010000056 DASSNH010000000
VERSION     DASSNH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816620"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..938)
                     /gene="modA"
                     /locus_tag="VFK00_02030"
     CDS             complement(108..938)
                     /gene="modA"
                     /locus_tag="VFK00_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7591766.1"
                     /translation="MRQLESTATTAAGGLMRCIERWFIGVAIAAGPLASAAAAAASPT
                     IFAAASLQPALDQMAHEGILGAPAPRLVYAASSALARQVEDGAPSDLFISADERWMDD
                     AAAHDAVVAASRTDLLGNGLVLIAPAGAHADADLAHGAAPLLDILGPGGRLAIALPDS
                     VPAGIYAEQALTHLGMWSALQPRMAMTRDVRAALNLVVLGQCPLGIVYRSDAASEPRV
                     RVLATFPASSHDPVVYPVAIVKGHDSPAARSLLAALQSGRAREVFHRWGFDTLPRTKH
                     "
     gene            complement(967..1503)
                     /locus_tag="VFK00_02035"
     CDS             complement(967..1503)
                     /locus_tag="VFK00_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7591767.1"
                     /translation="MRRLLPWLVLLLVPVMAFGAQGFSLTSRTLQDGGSMPIQGVYTE
                     CGGGNISPELMWHDPPKGTRSYAVTMFDPDAKGGFWHWVMFDISVGAQGLNAGAGTPF
                     SGDAPGDAVGLRNDFGNLGYSGPCPPPGPPHHYIITVFALDVPRLDFLAHFDRGAALA
                     QIRQHTIAKATLTVTWGR"
     gene            complement(1511..1891)
                     /locus_tag="VFK00_02040"
     CDS             complement(1511..1891)
                     /locus_tag="VFK00_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HET7591768.1"
                     /translation="MAFKIKRTTEPVGKDDGARILIDRLWPRGQSKERLELTAWVRDI
                     APSTELRKWFGHDPAKWEEFRKRYRAELDANPEAVAEVRALLEKHANATLLFAAHDEK
                     HANAVVLAEYLRGHAPRPRRKSMA"
     gene            1960..>2262
                     /locus_tag="VFK00_02045"
     CDS             1960..>2262
                     /locus_tag="VFK00_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET7591769.1"
                     /translation="MTRRLIVRRSPIHGNGVYAAVDIPAHTVLIQYKGRLLTHAQADR
                     LYDDGADTGHTFLFTLNDKYIVDANVGGNSARWINHSCAPNCCAYIQEVDGARKDKV"
BASE COUNT          372 a          755 c          763 g          372 t
ORIGIN      
        1 cattccaggc gctggacgtg tatgtgtggc caagtttcat ttcgtcattc cgggttctgc
       61 cccaacgagc aggggcagcc ccggaatgac gagggggagg gtgatcctca gtgctttgtg
      121 cgtggcagcg tatcgaaacc ccatcggtgg aacacctcgc gcgcgcgccc ggattgcagg
      181 gccgcgagca gcgagcgtgc ggcgggactg tcgtggccct tgacgatggc gaccgggtac
      241 acgacagggt cgtggctcga ggcggggaag gtcgcgagca cgcgcacgcg cggctccgac
      301 gccgcgtcag agcgatacac gatgccaagc gggcattgcc cgagtacgac gaggttcagc
      361 gccgcgcgca cgtcgcgcgt catggccatg cgcggttgca gcgccgacca cattccgagg
      421 tgcgtcagtg cctgctccgc gtagatgccc gccggcaccg aatcgggaag ggcgatcgcg
      481 agccggccgc cgggtccgag gatatccagc aacggtgctg caccgtgcgc gaggtcggcg
      541 tccgcgtggg cgccggccgg cgcgatcagc accagcccgt tgccgaggag atccgtccgc
      601 gacgccgcca cgacggcatc atgcgccgcg gcgtcgtcca tccagcgctc atcggcagaa
      661 atgaagaggt cggagggcgc accgtcctcg acttggcggg cgagcgccga actcgcggcg
      721 tacaccagcc gcggcgcggg cgctcccaag atgccttcat gcgccatctg gtcgagcgcc
      781 ggttgcaggc tcgccgccgc gaagatcgtg ggcgatgcgg cggccgccgc cgcggatgcg
      841 agtggaccgg ccgcgatggc cacgccgatg aaccatcgct cgatgcaacg catcaatccg
      901 cccgcagccg tggttgcggt cgattctagc tggcgcatcc ggacccgctc tcgccacgga
      961 gcggactcag cggccccagg tgaccgtgag cgttgccttt gcgatcgtgt gttgccttat
     1021 ctgggccaag gccgcgccgc ggtcgaaatg cgccaggaaa tcgagtcgcg gaacgtcgag
     1081 tgcgaagacc gtgatgatgt aatggtgggg cggccccggt ggtggacacg gacccgaata
     1141 acccaagttg ccgaaatcgt tgcgcaggcc cacggcatcg ccgggagcat cgccgctgaa
     1201 aggggtcccg gcgccggcat tgaggccctg ggcgccgacc gagatgtcga acatgaccca
     1261 gtgccagaag ccgcccttgg cgtccggatc gaacatcgtg acggcgtagc tcctggttcc
     1321 cttcggaggg tcgtgccaca tcagttccgg cgaaatgttg ccacccccgc attcggtgta
     1381 gacgccctgg atcggcatgc ttccgccgtc ctggagcgtg cggctggtca gcgaaaagcc
     1441 ctgcgcgccg aacgccatca ccggcaccag caacaggacc agccatggca ggaggcgtcg
     1501 catcgcgctt tcaggccatg ctcttccggc gcgggcgcgg cgcgtgcccg cgcaggtact
     1561 cggccagcac taccgcgttg gcgtgctttt cgtcgtgcgc cgcgaacaga agcgtagcgt
     1621 tcgcgtgttt ctcgagcagc gcccgcactt cggcgaccgc ctcggggttg gcatcgagtt
     1681 cggcgcgata gcgcttgcgg aattcctccc acttggcggg atcgtggccg aaccacttgc
     1741 gcagctcggt gctcggcgcg atgtcgcgca cccacgccgt gagctcgagt ctttccttcg
     1801 actggccgcg cggccacagg cggtcgatga ggatgcgtgc cccgtcgtcc ttgcccaccg
     1861 gctccgtcgt gcgcttgatc ttgaatgcca tggcaactcc cgtttgtgcg atcgccaccc
     1921 gacacggcat cataggcgtt tggccgtgaa tgttgtgcaa tgacaagacg attgattgtt
     1981 cgccgctcgc ccatccatgg caacggcgtg tacgccgccg tggacatccc tgcccacacc
     2041 gtgctgatcc agtacaaggg ccgcctgctg acgcacgcgc aggccgaccg cctgtacgac
     2101 gacggcgccg acaccggcca cactttcctg ttcaccctga acgacaagta catcgtcgat
     2161 gccaatgtcg gcggcaacag cgcgcgctgg atcaaccatt cctgtgcccc gaattgctgc
     2221 gcgtacatcc aggaggtcga tggcgcgcgc aaggacaagg tg
//
LOCUS       DASSNH010000057         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_203523, whole genome shotgun sequence.
ACCESSION   DASSNH010000057 DASSNH010000000
VERSION     DASSNH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203523"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFK00_02050"
     CDS             <1..1015
                     /locus_tag="VFK00_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HET7591770.1"
                     /translation="YGRATNPELSTLRNIVPLPRFITSWPESIAAIPMILTRRKPVMA
                     WDASWHEASILRAMSEAGYETYWISNQQAIGEFDSPVSMYAYEADHVEWLNRASWTAP
                     GSYDGDLVRPLEDALHASGNDLFIVLHMMGNHVQYDYRYPEAFEHWRPTQVSAPGEGS
                     RIERARNSYDNSILYTDRVLARVIGVLKQSGAVSALWFESDHGELIPTPTCDKEGHGV
                     GTVPEYEIPAFVWYSDSYEQDFPQRVAALRANAGKRTLSADTFESLVDMAGVTFPGHD
                     ETWSLFSLDWRYHTRWVAQTRHIDFDTARIDKGCERVMPGPAQNRGQSRVLARPLQNG
                     VSG"
     gene            1012..1659
                     /gene="coaE"
                     /locus_tag="VFK00_02055"
     CDS             1012..1659
                     /gene="coaE"
                     /locus_tag="VFK00_02055"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534337.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7591771.1"
                     /translation="MKLDSDPGFGDGHYVVALTGGVAAGKSAVSKRFEALGIGVYDAD
                     VAAREVVAPGTPALAEIEFMFGHGVLRADASLDRQAMRERVFADPEARKKLEAIIHPR
                     VRVWLRRKVASDRGAYCMLAIPLLVENQTEYEWVDRVLLVDVPEEVQVARLMARDGVT
                     REHAQSMLAAQSTREQRLAIADDVIVNDGEEAALEAKVAALHARYLELATARRPG"
     gene            complement(1891..2847)
                     /locus_tag="VFK00_02060"
     CDS             complement(1891..2847)
                     /locus_tag="VFK00_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HET7591772.1"
                     /translation="MIEVAAGILTDADNRVLLMQRLPGKHLAGLWEFPGGKLERAETI
                     EQALARELREELGIEVRACAPLISIPWRYPEKSVLLHALRVSEWTGEPRAREGHPLQW
                     MRVDAMDVARMPPADAPIVAALRLPPYYAIAEKPGSESLFGDGNPLPRSELKSDSDPG
                     FPPGPPLVQLRMPGASREAIREVVMQWLDSEPPLRRRLLVNHDVDLAGELGVGVHLRA
                     AQLRELRERPLPPTSWVGASCHDGEELELAAHLNADFATLSPVRSTASHPDAKPLGWD
                     GFARLVADARLPVYALGGVEPVDLERARLAGAQGVAGIRAFG"
     gene            complement(2844..3326)
                     /locus_tag="VFK00_02065"
     CDS             complement(2844..3326)
                     /locus_tag="VFK00_02065"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008576390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7591773.1"
                     /translation="MISLIAALDRNRAIGRAGVMPWHLSDDLKRFKALTLGKPVLMGR
                     KTALAIGRALPGRPNLVLTRSDAAPFADQTVVHSLDEALAHAAGAELCVIGGGEVYVL
                     ALPRATHLRLTEIDTVTPHADTFFPDYDRGEWREMFREHHPADARHALAFDFADYERR
                     "
     gene            complement(3482..4276)
                     /locus_tag="VFK00_02070"
     CDS             complement(3482..4276)
                     /locus_tag="VFK00_02070"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7591774.1"
                     /translation="MRIYLDLLRDVLEHGARKDDRTGTGTLSVFGRQIRMDLGAGFPL
                     LTTKKLHIRSSVHELLWFLRGDTNIAYLKEHGVRIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRTVDQVSRVIEQIRHNPDSRRMIVSAWNVGELESMALMPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTCMIAQVCGLERGDFVHTFGDLHLYSNHLEQARE
                     QLGRTPRALPKLTLNPDVSSIFDFRYEDIHVVDYDPWPAIKATVAV"
     gene            4568..>4975
                     /locus_tag="VFK00_02075"
     CDS             4568..>4975
                     /locus_tag="VFK00_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HET7591775.1"
                     /translation="MPTVSKTRSFSTVFLIEMWERFGYYGMQALIIYYMVQRLEFQDT
                     PANLTWGAAAALIYVSPAIGGWVGDKFFGTRHTMLLGAFVLAIGYALMVVPAHSTNFL
                     FLALGVIVVGNGLFKANAGNLVRKIYEGDDSKID"
BASE COUNT          875 a         1610 c         1646 g          844 t
ORIGIN      
        1 ctatggccgc gcgaccaatc ccgaattgtc gacgctgcgg aacatcgtgc cgctgccgcg
       61 gttcattacc tcgtggccgg agtcgatcgc cgcgatcccg atgatcctga cgcgccgcaa
      121 acccgtgatg gcgtgggacg cgtcctggca cgaagcatcg atacttcgcg caatgtcgga
      181 agccggttac gagacgtact ggatttccaa ccagcaggcg atcggcgaat tcgactcgcc
      241 ggtgtccatg tacgcctacg aggccgacca cgtcgaatgg ttgaaccgtg cctcctggac
      301 cgcgccgggc agttacgatg gcgacctggt gcggccactc gaggatgcgt tgcatgcatc
      361 cggcaacgat ctattcatcg tgctgcacat gatgggcaac cacgtgcaat acgactaccg
      421 ctatcccgaa gcattcgagc actggcgacc gacgcaggtg tccgcgccgg gcgagggctc
      481 gcgcatcgag cgggcacgca acagttacga caactcgatc ctttacaccg accgcgtcct
      541 ggcccgggtg atcggtgtgc tgaagcaaag cggtgcggtg agcgcgctgt ggttcgagtc
      601 cgaccacggc gaactgatcc cgacgccgac ctgcgacaag gagggccacg gcgtcggcac
      661 cgtgcccgaa tacgaaatcc ccgcgttcgt ctggtattcg gactcgtacg aacaggattt
      721 tccgcaacgg gtcgcggccc tgcgcgccaa tgccggcaag cgcaccctct ccgccgacac
      781 cttcgaatcg ctcgtcgaca tggcgggcgt gacgtttccc ggccacgacg aaacctggag
      841 cctgttcagt ctcgactggc gctaccacac gcgctgggtc gcgcaaacgc ggcacatcga
      901 tttcgatacg gcgcggatcg acaagggctg cgaacgggtc atgccgggcc ctgcgcaaaa
      961 ccggggtcag agtcgagttt tggcgcggcc gctacagaat ggcgtttccg gatgaaactc
     1021 gactctgacc ccggttttgg ggacggccat tacgtcgtcg cgctgaccgg cggcgtcgcc
     1081 gccggcaaga gcgccgtgtc caaacggttc gaggcgctgg ggatcggcgt gtacgacgcg
     1141 gatgtcgccg cgcgcgaggt cgtggcaccg gggacgcccg ccctggctga aatcgagttc
     1201 atgttcggcc acggcgtgct gcgagccgac gcatcgctgg atcggcaggc aatgcgcgaa
     1261 cgggtgttcg ccgatccgga agcgcgcaag aagctcgagg ccatcatcca tccgcgcgtg
     1321 cgcgtatggt tgcgtcgcaa ggttgcgtcg gaccgtggcg cctattgcat gctcgcgatc
     1381 ccgctgctgg tcgagaacca aaccgaatac gaatgggtgg accgcgtgct gctggtcgat
     1441 gtaccggagg aagtgcaggt cgcgcggctg atggctcgcg acggtgtcac ccgcgaacac
     1501 gcgcaaagca tgctcgccgc gcaatccacc cgcgagcaac gcctcgcgat cgccgatgac
     1561 gtgatcgtca acgatggcga ggaagccgcg ctggaggcaa aggtggcggc gttgcacgcg
     1621 cgatacctgg aactcgccac agcccgtagg ccgggttgac cgcggcttca tcgcgggagg
     1681 ccaatgttcc ttgcgagatt gctgttgggc ttccccccgg actaggtccg ggggtcaacc
     1741 caacctacgc gtcccagagg tggcccgtac ctcacgtcca gcgcaaggag cgggtaggtt
     1801 gggttgaccg cggctccatc gcgggaagcc caacgtggtg acgcgacaac gttgggcttc
     1861 ccccggacca ggtccggggt tcaaccgcac ctacccaaat gcgcggatgc cggcgacgcc
     1921 ttgcgcgccg gccaaacgag cgcgttcgag atccaccggt tcgacgccac ccaatgcata
     1981 caccggcagc cgcgcatcgg cgacgagcct cgcgaacccg tcccagccga gcggcttcgc
     2041 gtcggggtgc gatgcggtgc ttcgcaccgg tgacagggtc gcgaagtctg cattgaggtg
     2101 tgcggcgagt tcgagttctt cgccgtcatg gcacgaggcg cccacccagg aggtcggcgg
     2161 caacggacgt tcgcgcaact cgcggagttg tgccgcacgc aggtgcacgc ccacgcccag
     2221 ttcgcccgca aggtccacgt cgtggttgac cagcaggcgt ctccgaagcg gcggctccga
     2281 atccagccat tgcatgacga cctcgcgaat cgcttcccgc gacgcaccgg gcatgcgcag
     2341 ttgaacgagc ggaggaccgg gtggaaaacc ggggtcagag tcgcttttca attccgatcg
     2401 cggcaacggg ttgccatcgc cgaaaagcga ctctgacccc ggtttttccg cgatggcgta
     2461 gtacggcggc aggcgcagtg cggcaacgat gggcgcatcg gcagggggca ttcgagcgac
     2521 atccattgca tcgacgcgca tccattgcaa tggatgccct tcccgtgcgc gcggttcacc
     2581 cgtccactcg ctcacccgca gcgcgtgcag caggaccgat ttttccgggt agcgccaggg
     2641 aatggaaatc agcggcgcac acgcgcgcac ctcgatcccg agttcctcgc gcaactcacg
     2701 cgccagcgcc tgctcgatgg tttcagcgcg ttcgagcttg ccgcccggaa actcccacaa
     2761 acctgccagg tgcttgccgg gcagcctttg catcagcagc acgcggttat ccgcgtcggt
     2821 gaggatcccg gcggcgactt cgatcaccgg cgctcgtagt ccgcgaaatc gaacgcgagg
     2881 gcatgcctgg cgtcggcagg atgatgttcg cggaacatct cccgccactc gccgcggtcg
     2941 tagtcgggga aaaacgtgtc ggcgtgcggg gtgacggtgt cgatttcggt gaggcgcagg
     3001 tgcgtggcgc gcggcagcgc cagcacatag acctcgccgc cgccgatgac gcaaagttcc
     3061 gcaccggccg cgtgggcaag ggcttcatcg agcgagtgga ccaccgtctg atcggcgaac
     3121 ggcgcagcgt ctgaacgcgt cagcaccagg ttcgggcgcc ccggcaatgc acgcccgatc
     3181 gcaagcgcgg tcttgcggcc catcagcacg ggcttgccga gtgtcagtgc cttgaagcgc
     3241 ttcaggtcgt cgctgaggtg ccacggcatc acgccggcgc ggccgatggc acggttgcgg
     3301 tcgagggctg cgatgaggga gatcatgaat ggattgccgt ggccttgggg cgactacatc
     3361 cccccgccgc ttcgcggcaa acccccttcc tgcggaaggg ggggatcaac ttccgccttc
     3421 cgcaggaagg cggagcgaag cgaacgcgcg cgcagcgcga gagggggatg gctcttgatc
     3481 ttcacacggc cacggtggcc ttgatcgccg gccacggatc gtagtccacg acgtggatgt
     3541 cctcgtagcg gaagtcgaag atcgaggata cgtcgggatt cagggtcagc ttgggcagag
     3601 cacgcggcgt gcggcccaat tgttcgcgcg cctgctcgag atggttggag tagaggtgca
     3661 ggtcgccgaa ggtgtgcacg aaatccccgc gctcgagccc gcaaacctgg gcgatcatgc
     3721 aggtgagcag cgcgtagctc gctatgttga acggcacccc aaggaagatg tcggccgagc
     3781 gctggtagag ctggcacgac agttttccgt cggcgacgta aaactggaac agcgcgtggc
     3841 agggcatcag cgccatcgat tcgagttcgc ccacgttcca ggccgacacg atcatgcggc
     3901 gcgaatccgg gttgtgccgg atctgctcga tgaccctcga gacctggtcg accgtgcgac
     3961 cgtccggcgt cggccacgcg cgccactgct tgccgtacac cgggccgagg tcgccgtttt
     4021 cgtccgccca ctcgtcccag atgcggacgc cgtgctcctt gaggtaggcg atgttggtgt
     4081 cgccgcgcag gaaccacaac aactcgtgca cgctcgaacg gatgtgcagc ttcttggtgg
     4141 tcagcagcgg gaacccggcg ccgagatcca tgcggatctg tcgcccgaac accgacagcg
     4201 tgccagtgcc ggtgcggtcg tccttgcggg cgccgtgctc gagcacgtcg cggaggaggt
     4261 cgagataaat gcgcatgctg cgggaactgg gaactgggaa ctgagaactg ggaaagcata
     4321 gcagcggcct ggcacgcgcc tcctcggcca aagcccccat cgtagccagc tcaaggagcg
     4381 cagagccggc aatcaacacg gagatgccgg ttccggcctg cggccttgaa ccggcctact
     4441 ggttgctact ccctgttccc ccgttccctg ttcccgctct tcgctccgcc gccacattca
     4501 gccacggttc gggtaggctt ggccgatcgc tctgctgcag gggtgtcccc cgaacatgaa
     4561 ttccgcaatg cccaccgttt ccaagacccg ttcgttctcg accgtgttcc tgatcgagat
     4621 gtgggagcgc ttcggctatt acggcatgca ggcgctgatc atctactaca tggtgcagcg
     4681 gctggagttc caggacaccc cggccaacct gacgtggggc gcggccgcgg cgctgatcta
     4741 cgtctcgccc gcgatcggcg gctgggtcgg cgacaagttc ttcggcaccc gccacaccat
     4801 gctgctgggc gccttcgtgc tggcgatcgg ctatgcgctg atggtggtgc cggcccacag
     4861 caccaacttc ctgttcctcg cgctcggcgt gatcgtggtc ggcaacggcc tgttcaaggc
     4921 caacgcgggc aacctggtcc gcaagatcta cgagggcgac gacagcaaga tcgac
//
LOCUS       DASSNH010000058         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_306490, whole genome shotgun sequence.
ACCESSION   DASSNH010000058 DASSNH010000000
VERSION     DASSNH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306490"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1642
                     /locus_tag="VFK00_02080"
     CDS             257..1642
                     /locus_tag="VFK00_02080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7591776.1"
                     /translation="MPAGSRCTRTPAWDDLVKALRGGLLLPGNPDYETRRRVWNGAID
                     RRPLAIARCVEAADVAATVKFAAREHLPMTVRGGGHNVAGLAVREDALMLDLGAMNRV
                     EVDAPARVARVEGGTLWRELDAATQPHDLATTGGFVSTTGVGGYTLGGGVGWLMRRCG
                     LAIDNLLEVEVVLADGRIVTASGTQHPDLFWGLRGGGAGLGVVTSFTFRLHPVGMVHA
                     GVAFYPMDTAHALLRAFRAFATDATDAATAMLVFTTAPPLPFLPVAVHGQRTVALAYC
                     WSGDTAQGTGAAAPIAETAEPLGRHEGVMPYAAWQQAFDPAAPPGHHYYWTTCQFDAF
                     DDALIAALVARAAKPADPLCEVHVHHLGGAVARVARDATAFSYRDAPFFINVIGHTAT
                     AEGFTVVRDWVRELRTAFAPHARAGMQPNFAGELSDLEANAHDTGTRSRLSALRARYD
                     PEGLLAPLRSA"
     gene            complement(1600..4113)
                     /locus_tag="VFK00_02085"
     CDS             complement(1600..4113)
                     /locus_tag="VFK00_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7591777.1"
                     /translation="MEKHGDAGLTTGTFAFADVTIDAGAHRLSRGGRDIAVEPKAFAV
                     LLQFLAHPDQLLTRDQLLDAVWGHGFVTPATLNRIIAQLRKALADDSEAPRCIQTVHG
                     LGYRFIAPLQTIPGSAVPALRFAPPPRARLPERTEPLIGRDADIEALKQMLREHRLVT
                     LTGPGGLGKTQAALETARAVAADFPDGVWLFDCTPHTDGDALVRWLADMFDIHATTDA
                     AQLIARLGELLQTRRVLLVFDNCERIAAPLGTAVANLLSAGAEVRILVTSQQRLNCAG
                     ESLYGLPPLQVPPPGEWTTDEQLACLASIAAVRLLVLRSQACASGFTLTRVNAATVAE
                     ICRRVAGLPLALELAAARLRLLSPEQLLARMDARLLNLAESNPGRPARHQTLRALIEW
                     SFALLSEKEQSLLCGLSIFVGACTLGGATAIGAALGLEDPQTLELLAGLIDKSLLTVD
                     AATNPPSYRLLDSVRLFAQRQLVESGNEMRVRTAHLAHFVQLTERINIEIRSNRFQLW
                     FERVRREWNNLHAAFDFALGQPGLADDALAIVASLCWYFRARSDYVQSAQLLDSVLRA
                     TSKDAPHRALALIALGMVSHQSQMHERAAACLREGIALATGRNDAWLAAAGRAILAFE
                     LATCGDFDRATGEVESALGVAEALDDRWLHSQALLSRGIICGLQGRHREGETWLGEAA
                     ALVASPPNDAFQQVYALINRALLRHYLGNAPGAARDWLDVLDGCVMLQNRRGASGCVE
                     GTAYLAVERGEPEKAVRFLASAARVRELTGAPLMPLWRKGQQATERRVRAMLGDAFEV
                     GQRAGAAARFEDIIDEARAFLAETAGQAERSGASNPSGS"
     gene            4275..4952
                     /locus_tag="VFK00_02090"
     CDS             4275..4952
                     /locus_tag="VFK00_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591778.1"
                     /translation="MNTRLYACAAMAVIGLSGIAATRQADACGLNLVKSANGQWKIAP
                     PAKLSAAAARMKFLAAIPTANVKAMPNPLQYLAPIAGLYEVTMTAEGNGPTGLPDGAQ
                     VDHAYVAWHADGNEIMNSGRPAADANFCMGTWVQTGARTYTLKHFMLSWGQSVDADGV
                     HVDNTFVGPASIQETITLSKDGSSYTGTFVLTQYDTNYNIILPGSIPIKGTVSGTRKT
                     INSPVTY"
BASE COUNT          854 a         1770 c         1686 g          799 t
ORIGIN      
        1 cacaggtaac aggccacgca ggcgaccacc agcaccgggg taacccagcg ggggtggacc
       61 tgcttgcggc tgggcacgtc gacgaacacg gcacatacat tagcgggttg catggggacg
      121 gcagccaccg ccctcgcgtt ccctacgaaa tcaccccatc tccatcacga ccgcagccgg
      181 ccgcacgcgt atagtgcgcc acgcatcgac ttgcggccgg gcgtctcgcg cgccagccac
      241 agacaggagc actgccatgc ccgctggatc ccgctgcacc cgcactcccg cgtgggacga
      301 tctggtcaaa gcgctccgtg gtggcctgct gctgccgggc aatcccgact acgaaaccag
      361 gcgccgcgtc tggaacggtg cgatcgaccg gcgtcccttg gcgatcgcac gctgcgtcga
      421 ggccgcagac gtcgccgcga cggtgaaatt cgccgcgcgt gaacacctgc cgatgacggt
      481 gcgcggcggc ggccacaacg tggccgggct ggcggtgcgc gaggacgccc tgatgctgga
      541 tctgggcgcg atgaaccgcg tggaagtcga tgcgccagca cgcgtggctc gcgtcgaagg
      601 tggcaccctg tggcgcgagc tggatgcggc aacacaaccg cacgatcttg ccaccaccgg
      661 cggtttcgtc tccaccaccg gcgtcggcgg ctacacgctg ggcggcggcg tcggctggct
      721 gatgcgccgc tgcggtctcg cgatcgacaa cctgctcgaa gtggaagtcg tgctggccga
      781 cggccgcatc gtcacggcca gcgggacgca gcatcccgac ctgttctggg gcctgcgcgg
      841 cggcggggcc ggcttgggcg tcgttacaag cttcactttc cggctgcatc cggtcggcat
      901 ggtccacgcg ggcgtggcgt tctatccgat ggacacggcg cacgcgttgt tgcgcgcatt
      961 ccgtgcgttc gcaacggacg cgacggacgc cgccaccgcg atgctggtgt tcaccaccgc
     1021 gccgccgttg ccgttcctgc cggttgcggt gcatgggcag cgcacggtgg cgctggcgta
     1081 ttgctggagc ggcgatacgg cgcagggcac gggcgccgcg gcgccgatcg cggagactgc
     1141 cgagccgctg ggccgccacg aaggcgtgat gccttacgcg gcatggcagc aagccttcga
     1201 tcccgctgcg ccgcccggcc atcattacta ctggaccact tgccagttcg acgcgttcga
     1261 cgacgcgctg atcgcggcgc tggttgcccg cgccgcgaag cccgccgacc cgctgtgcga
     1321 agtgcacgtg catcacctgg gcggtgcggt cgcacgcgtc gcccgcgatg ccaccgcatt
     1381 ctcgtatcgc gatgcgccgt tcttcatcaa cgtcatcggt cacaccgcca ccgcggaagg
     1441 cttcacggtt gtccgcgact gggtgcgcga gctgcgcacg gcgttcgcgc cgcatgcgcg
     1501 tgcgggcatg cagccgaatt ttgcgggtga actttccgac ctggaagcga acgcgcatga
     1561 caccggaacc cgttcccggt tgtcggcttt gcgcgcacgc tacgacccgg aaggattgct
     1621 ggcgccgctg cgctccgcct gaccggccgt ttccgcgagg aacgcgcgcg cttcatcgat
     1681 gatgtcctcg aagcgcgccg ccgcgccagc ccgctgccca acttcgaacg cgtcgccgag
     1741 catggcgcgt acccggcgct cggtcgcctg ctggcccttt cgccacagcg gcatcaacgg
     1801 tgcgccggtc aattcacgta cgcgcgcggc ggaggcgaga aaccgcaccg ccttttcggg
     1861 ttcgccgcgc tcgacggcga ggtaggccgt accctcgacg cagcccgaag cgccccgcct
     1921 gttctgcaac atgacgcagc catcgaggac gtccagccag tcccgtgccg ccccgggcgc
     1981 gttgccgagg taatgccgca gcaaggcgcg gttgatcagc gcgtagacct gctggaaggc
     2041 gtcgttgggc ggcgaggcga ccaacgcggc ggcctcgccg agccaggtct cgccctcgcg
     2101 atgacgacct tgcaatccgc aaatgatccc gcggctcagc aatgcctgag aatgcaacca
     2161 cctgtcgtcc agcgcctcgg cgacgccgag cgccgattcc acttcaccgg ttgcgcgatc
     2221 gaaatcgccg caggtcgcga gttcgaaagc caggatcgcg cgtcccgccg cggcaagcca
     2281 cgcgtcgttc ctgcccgtgg ccagcgcaat gccctcgcgc agacacgcgg cggcgcgttc
     2341 gtgcatctgg ctctggtggc tgaccatgcc cagcgcgatc aacgccaacg cacgatgcgg
     2401 cgcatccttc gaggtggcgc gcagcaccga atccagcagt tgcgcggatt gcacgtaatc
     2461 cgagcgcgcc cggaaatacc agcaaaggct ggcgacgatc gccaacgcgt cgtcggcaag
     2521 gcccggctgg cccagtgcaa aatcgaacgc cgcgtgcagg ttgttccatt cgcgcctgac
     2581 gcgttcgaac cacaactgga agcggttgct gcggatctcg atgttgatgc gctcggtgag
     2641 ctgcacgaaa tgcgcaaggt gcgcggtgcg cacccgcatt tcgttgccgc tttcgaccag
     2701 ttggcgctgc gcgaacaagc gcacactgtc gagcaggcga taggacggcg gattggtggc
     2761 ggcatcgacg gtcagcagcg acttgtcgat caggccggcc agcaactcga gggtctgcgg
     2821 atcctcgagt cccagcgccg cgccgatcgc ggtggcgccg ccgagcgtgc aggcaccgac
     2881 gaagatgctc agtccgcaca gcaacgattg ctccttttcg gagagcaacg cgaaactcca
     2941 ctcgatcagc gcacgcagcg tctggtggcg cgcgggacgg ccggggttgg attcggccag
     3001 gttgagcagc ctggcatcca tccgcgccag taactgctcg ggactcaaca ggcgcagccg
     3061 cgcggccgcc agttccagcg ccagcggcag gcccgcaaca cggcgacaga tctcggcgac
     3121 cgtggcggcg ttgacgcgcg tcagcgtgaa ccctgaggca caagcctgcg agcgcagcac
     3181 cagcaatcgc accgcagcga tcgacgccag gcatgcgagc tgctcgtccg tcgtccattc
     3241 gcctggcggc ggcacctgca ggggcggcaa tccgtacagc gattcccccg cgcagttcag
     3301 gcgctgctgg ctggtcacca ggatgcgcac ttcggcgccg gctgacagca ggttcgcgac
     3361 cgcggtgccg agtggcgcgg cgatccgttc gcaattgtcg aacaccagca gcacccgacg
     3421 cgtctgcagc agctcgccca gccgcgcgat cagttgcgcc gcgtcggtcg tggcatggat
     3481 gtcgaacatg tccgccagcc atcgcaccag cgcgtcgccg tcggtatgcg gcgtgcagtc
     3541 gaacagccat acaccgtcgg gaaagtcggc ggcgaccgca cgcgcggttt ccagcgcggc
     3601 ctgcgtcttg ccgaggccgc cggggccggt gagggtgacc agccgatgct cccgcagcat
     3661 ctgcttcagc gcctcaatgt cggcatcgcg gccgatcagc ggttcggtac gttccggcaa
     3721 tcgcgcacgc gggggcggcg cgaagcgcaa cgcgggaacc gcgcttcccg ggatggtctg
     3781 cagcggggca atgaaccggt agccgaggcc atgcacggtc tggatgcagc gcggcgcttc
     3841 gctgtcgtcg gcgagcgcct tgcgcaattg cgcgatgatg cggttgagcg tggcgggggt
     3901 gacgaaaccg tgtccccata ccgcatccag caactggtcg cgggtcagca actggtccgg
     3961 gtgcgccagg aattgcagca ggaccgcgaa agccttcggc tcgacggcga tgtcacggcc
     4021 gccacgcgac aggcgatgcg cgcccgcgtc gatggtcacg tcggcgaaag cgaacgtgcc
     4081 cgtcgttagt ccggcatctc cgtgcttttc catgccgccg cgcgctccac gcgaatcacg
     4141 tcaacgccac cgccgcactc tcgcgatttc cctaccgaaa catcatgact cgtgggtttg
     4201 ccgggcgcag tttatacctc cggatcgcgg tgcatgccgc catccgccgg gaccatcttt
     4261 ttgaggagaa cgccatgaat acccgcttgt acgcctgcgc tgcgatggcc gtgatcggcc
     4321 tgtccggtat tgccgcaacc cgccaggccg acgcctgcgg gttgaacctg gtgaagtccg
     4381 ccaatggcca gtggaagatc gcgccgcccg ccaaactctc ggcggctgcg gccaggatga
     4441 agttcctggc cgccatcccg accgcgaacg tgaaagccat gcccaatccg ctgcaatacc
     4501 tcgcacccat cgcgggcctg tacgaagtca ccatgaccgc cgagggcaac gggccgaccg
     4561 gcctgccgga cggcgcccag gtcgatcacg cctacgtggc gtggcacgcg gacggcaacg
     4621 aaatcatgaa ttcgggcagg cctgccgcag atgccaactt ctgcatgggc acatgggtgc
     4681 aaacgggcgc gcgaacctac acgctcaagc atttcatgtt gtcgtggggg cagagcgtgg
     4741 acgcggatgg cgtccatgtc gacaacacgt tcgtcgggcc agccagcatc caggagacca
     4801 tcacgttgag caaggacggg agttcgtaca ccgggacatt cgtgctcacc cagtacgaca
     4861 cgaattacaa catcatcctt cccggcagca tccccatcaa gggcaccgtg tcggggaccc
     4921 gcaagaccat caattcgccg gtgacgtatt gacgggatcg gtgcgaaaag gcgcggcgcg
     4981 tcagggcgcc gcgccttttt tcatgcgccg accagccgca cccgcgcgaa attccgcttg
     5041 cccacctgca gcacgccttc gaagcctgcc gtgaacacgc gttgcggatc gtccaccacc
     5101 tgcgcgtcg
//
LOCUS       DASSNH010000059         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_306025, whole genome shotgun sequence.
ACCESSION   DASSNH010000059 DASSNH010000000
VERSION     DASSNH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306025"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="miaA"
                     /locus_tag="VFK00_02095"
     CDS             <1..888
                     /gene="miaA"
                     /locus_tag="VFK00_02095"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7591779.1"
                     /translation="LACALAERFPVELVSVDSALVYRGLDIGAAKPDAATLARHPHAL
                     IDVRDPADPYSAALFRDDALRVMQAIAARGKLPLLVGGTGLYFRALERGLSAMPEANP
                     EVRDRLRGEAARTGWPALHARLAGRDPEAAARIRPNDAQRIQRALEAIELSGRPLSEL
                     HADRDAAERFPYRVLKLALLPDRGILRERIAARFDAMLAAGFLGEVRRLRERGDLGPE
                     LPAIRAVGYRQAWQHLDAAFDAAEFRERAIHATRQLAKRQTTWLRSELDARVFDPDRP
                     GCLRSLAGAVALFLGPEAA"
     gene            970..1245
                     /gene="hfq"
                     /locus_tag="VFK00_02100"
     CDS             970..1245
                     /gene="hfq"
                     /locus_tag="VFK00_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET7591780.1"
                     /translation="MSKSQSLQDPFLNALRRERIPVAIYLVNGIKLQGVIESFDQFVV
                     LLRNQVSQMVYKHAISTVVPSRAVKVGGDEATATDTKDDDAKPPAQD"
     gene            1251..2603
                     /gene="hflX"
                     /locus_tag="VFK00_02105"
     CDS             1251..2603
                     /gene="hflX"
                     /locus_tag="VFK00_02105"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HET7591781.1"
                     /translation="MFERERKGERALLVLPHSGNRLDARRVREFTELAQSAGAEILGS
                     VRARVDTPNPKFYIGTGKADVVQAMARELGADLVLVDHALTPVQERNLEKHLSARVVD
                     RSALILDIFAQRAHSHEGKLEVELAQLKHMATRLVRGWTHLERQRGGSIGLRGPGETQ
                     LETDRRLLQKRVEQLQKRIDRIGVQRAQQRRARLRNTLPRIALVGYTNAGKSTLFNAI
                     TRGEVFVADRLFATLDPTVRKLEDLASGPAVLADTVGFVRELPHDLVAAFRATLSEAR
                     DADLLLHVTDAADPERDVLIDVVNGVLDEIGAGEVPQLMVFNKIDLLRADVPGDQEAV
                     DGDREPRAASPRIDRDKDAAPRAVWLSAATGEGLDLLKQAIGERLAGSRVHAELRLPP
                     RAGRLRARLIEIGAVKSEHYDDEGWRLDIDFPRDLLLPLAGDVRGDDARLFRDWLDAA
                     "
BASE COUNT          477 a          991 c          990 g          456 t
ORIGIN      
        1 ctggcctgcg cgctcgccga gcgctttccc gtcgaactcg tgagcgtcga ttccgcgctc
       61 gtctatcgcg gtctcgacat cggcgcggcc aagccggatg cggcaacgct ggcgcgccat
      121 ccgcacgccc tgatcgatgt ccgcgatccg gccgacccgt attcggccgc gctgttccgc
      181 gacgacgcgt tgcgggtgat gcaggcgatc gccgcgcgcg gcaagctgcc gctgctggtc
      241 ggcggcacgg gcttgtattt ccgggcactc gagcggggcc tgtcggcaat gcccgaggca
      301 aacccggaag tccgtgatcg cctccgcggg gaagcggccc gcaccggctg gccggcgctg
      361 cacgcccgtt tggccgggcg cgatcccgaa gcggccgcgc gcatccggcc gaacgacgcc
      421 cagcgcatcc agcgtgccct ggaggcgatc gagctctcgg gccgcccatt gagcgaattg
      481 cacgcggacc gcgatgcggc ggagcgcttc ccgtatcgcg tcctgaagct cgccttgctt
      541 cccgatcgcg gcatcctgcg cgaacgcatc gccgcacgtt tcgatgcgat gctcgcggcg
      601 ggcttcctcg gcgaggtgcg gcgcctgcgc gaacgcggcg atctcgggcc cgagctgccg
      661 gcgattcgcg cggtcggcta ccggcaggca tggcagcatc tcgacgccgc gttcgatgcc
      721 gctgaattcc gcgaacgggc gatccacgcc acccggcagt tggccaagcg ccagacgacg
      781 tggttgcgga gcgagctgga cgcgcgggta ttcgatcccg accggcccgg ttgcctccgt
      841 tcgctggccg gcgcggtcgc gctgtttctc ggcccggaag ccgcctgagc aggccaatcc
      901 aggccgattt gcgctaccat cgcaaagcag ggagaggttt gccgcgaccc ggatcggaag
      961 gccataacaa tgtcaaaaag ccagtcgttg caggatccgt tcttgaatgc cttgcgccgc
     1021 gagcgcattc cggtggcgat ctacctcgtc aacggcatca agctgcaggg cgtgatcgaa
     1081 tcgttcgacc agttcgtggt gctgttgcgc aaccaggtga gccagatggt ctacaagcac
     1141 gccatctcga ccgtggtacc cagccgcgcg gtcaaggtgg gtggcgacga agcgacggcg
     1201 accgatacca aggacgatga cgccaagcca ccggcgcagg attgacggcc ctgttcgaac
     1261 gcgaaaggaa aggcgaacgt gcattgctgg tgttgccgca ctccggcaac cgcctcgatg
     1321 cgcggcgcgt gcgggaattc accgaactcg cccagtctgc cggtgcggaa atcctcggca
     1381 gcgtgcgcgc gcgcgtcgac accccgaacc cgaagttcta catcggcacc ggcaaggcgg
     1441 atgtggtgca ggcgatggcg cgcgaactcg gcgccgacct cgtgctggtc gaccacgcac
     1501 tcacgccggt gcaggaacgc aacctcgaaa aacacctgtc ggcgcgggtc gtcgatcgct
     1561 cggcactgat cctagacatc tttgcgcagc gcgcccactc gcacgaaggc aagctcgaag
     1621 tcgaacttgc ccagctgaag cacatggcga cgcggctggt gcgcggctgg acccacctgg
     1681 agcgccagcg cggcggctcg atcggtctcc gcggtccagg cgaaacccag ctcgaaaccg
     1741 accgccgttt gctgcagaaa cgcgtcgagc aattgcagaa gcgcatcgac cggatcggcg
     1801 tgcagcgcgc ccagcagcgg cgcgcgcgcc tgcgcaacac gctgccgagg atcgcgctgg
     1861 tcggctacac caacgccggc aaatcgacct tgttcaacgc gatcacgcgt ggcgaagtgt
     1921 tcgtcgcgga ccggttgttc gcgacgctcg accccacggt gcgcaagctc gaggacctcg
     1981 cgagcggccc ggcggtattg gccgataccg tgggtttcgt ccgcgagctg ccgcacgacc
     2041 tggtggcggc cttccgggcg acgctgtccg aagcacgcga cgccgacctg ctgctgcacg
     2101 tcaccgacgc ggccgatccc gaacgcgacg tgctgatcga cgtcgtcaac ggcgtgctcg
     2161 atgaaatcgg cgcgggggaa gttccgcagt tgatggtgtt caacaagatc gatttgctgc
     2221 gtgccgatgt gcccggagac caggaagcgg tcgatggtga tcgagaaccg cgcgccgcat
     2281 caccgcgcat cgatcgcgac aaggacgccg cgccgcgcgc ggtgtggctg tccgccgcta
     2341 ccggcgaagg cctcgacctc ctgaagcagg cgatcggcga acggctggcg ggttcgcggg
     2401 tgcatgcgga gttgcggctg cctccgcgcg cgggccggtt gcgcgcgcgt ttgatcgaga
     2461 tcggcgccgt gaagtccgag cattacgacg acgaaggctg gcggctcgac atcgattttc
     2521 cgcgagacct gctgctgcct ctggccggag acgtccgtgg cgacgacgcc cgcctgtttc
     2581 gcgattggct cgacgccgct tgatacggca cggaccatcc cggtcgccag caggagcgcg
     2641 ccatgcgcgc aatgggcgat ctggcaaacc gtagatgcag cagcgcggcg gactgtgtcc
     2701 gccctgcgta tagcggtgac tctttggtgt gtttccatcg cggccatggc cgctcccgca
     2761 acggcccgga gaccctgggc cgcccgccat gcgccccgca acggtctggt aaactgcaac
     2821 gtttgcgccg atcgcggcgc gcgatccgca atccgagagt gttgaaccat gccctggaag
     2881 gagcccggcg aaaaaccgcg tgaaccccgg ggcc
//
LOCUS       DASSNH010000060         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1749526, whole genome shotgun sequence.
ACCESSION   DASSNH010000060 DASSNH010000000
VERSION     DASSNH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749526"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VFK00_02110"
     CDS             complement(<1..924)
                     /locus_tag="VFK00_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7591782.1"
                     /translation="MNAIAPIEQVPTAVGAGALAERVQAKYADRITGTLTIPASAGNL
                     VSLPGDLPDELALALRSSDIQQLYSHQAEAWRAAARGENLVVITPTASGKSLCYTLPV
                     VASVMRELAKALYLFPTKALAQDQVADLIELSKAGSLGIRAATFDGDTPGDQRQAIRL
                     NGDIVISNPDMLHQGILPHHTKWAQFFESLKFIVIDEIHSYRGVFGSHLANVIRRLKR
                     ICAFYGTKPQFILCSATIGNPKEHAEALLGEPVTAITRSGAPSGEKRVLLWNPPVVNP
                     DLGLRASARSQSNRIARLAIRSGLKTLVFAKS"
     gene            complement(1106..1414)
                     /locus_tag="VFK00_02115"
     CDS             complement(1106..1414)
                     /locus_tag="VFK00_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591783.1"
                     /translation="MANRWAFQMGIRRVEVRAEWRRYGSAKALTIRNHQMFGLQPHGV
                     IAFPGDAHSLTVVQLAQAFRVPVLDAQALLAKQERRAAKATLCEGAPGASAIHNPWRA
                     "
     gene            complement(1580..2467)
                     /locus_tag="VFK00_02120"
     CDS             complement(1580..2467)
                     /locus_tag="VFK00_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET7591784.1"
                     /translation="MQNLMHGLEAVDHGQKAAIARELLADEERAPLRRPKVRWRWGLA
                     GAVAAVVVAALVLVGLDRHRGVEAATFVAQYATPHGQNRTISLPDGTSVTLGADSVLR
                     VQYRAEARSLDLVSGEAFFTVRHDASRPFVVQVGVARIEDLGTQFDVRRDSDQVVVAV
                     ASGVVSVAYSEATAPAMDARYPIAPVDVTAGREVVMATATPNRPIQHRVVDGTVGAWR
                     TGRLQFRDTPLGTVVANINRYSATPIVLDGPGVAQLRFTGTIYVDRIETWLKAVQKAL
                     PVSEQGRSDSNIVLALKGS"
     gene            complement(2653..>3279)
                     /locus_tag="VFK00_02125"
     CDS             complement(2653..>3279)
                     /locus_tag="VFK00_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7591785.1"
                     /translation="LDRWLPHQSSGSGRRALPYQLLEDAPESPRWAEPRADRDEAARS
                     ESVQKLFFEHNRALVRFLAAKLNSEAEARDVAQEAYVRLLQLDRPDAIGFLRTYLFRV
                     AENIANDHLRHRAVHERSQPTQQVLFEQLLNRPGPDRSAMGQEELQIVRQALRDLPAK
                     CRKAFVLHVFAERPLAEIAVEMQLSDRMIRYYVARAMAHCSAALDTTR"
BASE COUNT          563 a         1063 c         1053 g          600 t
ORIGIN      
        1 ggacttggca aagaccagcg tcttcagtcc cgaccggatc gccaagcggg caatgcggtt
       61 cgactgcgag cgtgccgagg cgcgaaggcc aaggtccgga ttcaccaccg gcggattcca
      121 caggagcacg cgcttctcgc cggaaggtgc gccggaccgg gtgatggcgg tgaccggctc
      181 gcccagcaag gcttcggcgt gttccttcgg gttgccgatc gttgccgagc acaggatgaa
      241 ctgtggtttc gtcccataga acgcgcagat gcgcttcagg cgccggatca cattcgcaag
      301 gtgggagccg aacacgccgc ggtagctgtg gatctcgtcg atgacgatga acttgaggct
      361 ttcgaagaac tgcgcccact tggtgtggtg gggcaggatg ccctggtgca gcatgtcggg
      421 gttcgagatg acgatgtcgc cgttaaggcg gatcgcctgg cgctggtcgc ccggggtatc
      481 gccatcaaaa gtggctgccc ggatgccgag cgatccggct ttggaaagct cgatcaggtc
      541 cgccacctgg tcctgcgcca gcgccttggt cggaaagagg tacagcgcct tggcgagctc
      601 gcgcatgacg gatgccacga ccggcagcgt gtagcacagc gatttgcccg acgcggttgg
      661 cgtgatcacc accaggttct cgccgcgtgc agcggcacgc cacgcttctg cctggtggct
      721 gtagagctgc tggatgtcgc tggacctcag agcgagcgcg agttcatcgg gcagatctcc
      781 cggaagggaa acaaggttgc cagcgctggc tgggatcgtg agcgtgcccg tgatccggtc
      841 cgcatacttg gcctggacac gttctgcaag cgcgcccgca cccacagcgg tcggtacttg
      901 ttcaatcggt gcaatggcgt tcatgccggc acgatgcccg ctgcaggtct caatagttga
      961 gacgtcgcgg tcgggcaaca tgctggtcaa actcctcgcg cgggatggcc tttagtgcag
     1021 tagggaaatt cctacaccac gccaagccgt cgcccgatac cgcgggccgc tattccaatc
     1081 cagaatgctc atgcgccggg tgttgtcagg cccgccatgg attgtggata gccgacgctc
     1141 ccggcgcgcc ttcacaaagg gtcgctttgg ccgcccgcct ctcctgcttg gcgagcaggg
     1201 cttgggcgtc caggaccggc acccgaaacg cttgtgccaa ctgcaccact gtcagtgaat
     1261 gcgcgtctcc cggaaacgca ataaccccat ggggctgaag cccgaacatc tggtggttgc
     1321 ggatcgtcag cgccttggcg cttccatacc gccgccattc cgcgcgcacc tcgacgcggc
     1381 ggatgcccat ctggaaagcc caccgattcg ccatctgctc cgccccgcgc tggctgtcgt
     1441 ggataacttc cgcgagggcc gcgcccttcg tgcccgtacc agagcagggg gaaagcatcg
     1501 tcttggcgtt gaagtcgcgt ccactacagc aagagtcgcg taaccggccg gtgtcgtgtc
     1561 cgagctccga ctctccgcct cacgaacctt tcaatgcaag cacgatgttc gaatcgctac
     1621 gcccttgttc gctgaccgga agggccttct ggaccgcctt cagccaggtt tcgatgcggt
     1681 ccacatagat cgttccggtg aagcgcaatt gcgcgacgcc agggccgtcg agcacgatcg
     1741 gcgttgcgga gtaacggttg atgttggcca ccacggtgcc gagaggcgta tcgcgaaact
     1801 gcaggcggcc cgtccgccat gctcccacgg tgccgtcgac gacgcgatgc tggatcggcc
     1861 gattgggggt ggccgtggcc atcacgactt cccggcccgc cgtcacgtcg acaggagcaa
     1921 tcggatatcg ggcgtccatt gcaggtgcgg tggcctccga ataggcgacc gataccacgc
     1981 ccgaagcaac ggccacgacc acctgatcgc tgtcacggcg cacgtcgaac tgcgtcccca
     2041 ggtcttcaat cctcgccacg ccgacctgga ccacgaaggg cctgctggcg tcgtggcgga
     2101 ccgtgaagaa ggcttccccg gatacgagat ccaatgatcg agcctcggcg cggtactgca
     2161 cgcgcaggac ggaatcggcg cccaacgtca cactggttcc gtcgggcagg ctgatggttc
     2221 ggttctgccc gtggggcgtg gcgtattgcg ccacgaacgt ggccgcttcg acgccgcgat
     2281 ggcgatccaa accgaccagc accaaggcgg cgacgacgac ggccgccact gcgcctgcga
     2341 gtccccatcg ccaccgcacc tttggcctcc gcaacggcgc ccgctcctca tccgcgagca
     2401 actcgcgcgc aatcgccgcc ttttgtccat ggtccaccgc ttccagacca tgcatcaggt
     2461 tctgcatgcg ctcgagcgca tcgagattgc gcgggtcgcc ctgcgaccag cgcaaccagt
     2521 tccggagttg gtgcgaatcc agctcctggt ggcggacgcg aaggagccac gaggctgcct
     2581 tgtgcaggcg ccgcatcgac agtgccggtc gcgatccgcg atcgtgcgcg tcttgtgtct
     2641 tttcgtccat ggtcatctgg tcgtgtccaa ggccgcactg caatgtgcca ttgcacgcgc
     2701 gacgtaatag cggatcatcc gatccgacaa ttgcatttcg acggcgatct ccgccagcgg
     2761 gcgttcggcg aacacatgca ggacgaacgc cttgcggcat ttcgcaggca ggtcgcgcag
     2821 cgcctgtcgt acgatctgca gttcctcctg gcccatcgcg gagcggtcgg gccccggtcg
     2881 gttcaagagt tgttcgaaca gtacttgttg cgtcggctgg ctgcgctcat gtaccgcgcg
     2941 atgccgcagg tgatcgttgg cgatgttctc ggcgacccgg aacaaatacg tgcgcaggaa
     3001 cccgatagcg tcaggacggt cgagctggag gagtcgcacg taggcctcct gcgccacgtc
     3061 ccgggcctcc gcttccgagt tcagcttggc ggcaaggaac cggaccagcg cgcggttgtg
     3121 ttcgaaaaac agtttctgca ccgactcgga acgggcggct tcatcgcggt ccgcgcgcgg
     3181 ctccgcccat cgcggcgact ctggcgcatc ttcgagcagt tgatagggca atgcccgtct
     3241 ccccgagccg gatgactggt gcggcagcca gcggtccaa
//
LOCUS       DASSNH010000061         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_304236, whole genome shotgun sequence.
ACCESSION   DASSNH010000061 DASSNH010000000
VERSION     DASSNH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304236"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFK00_02130"
     CDS             complement(<1..489)
                     /locus_tag="VFK00_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET7591786.1"
                     /translation="MLLAIRLAILALIVLFLLSALAVHFRSRVRLRLRKQLASHATVF
                     APYNLLMYAFSAVPGIAFPDRRGFPDVDLLRDNWKTIRDEAAGLSDQGHIRAALNDDD
                     ASFSSFIKVGWKRFYVKWYGEPLPSAQKLCPKTVELVNRIPRVKAAMFALLPPGATLN
                     PHR"
     gene            complement(530..1336)
                     /gene="thiD"
                     /locus_tag="VFK00_02135"
     CDS             complement(530..1336)
                     /gene="thiD"
                     /locus_tag="VFK00_02135"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7591787.1"
                     /translation="MAAPRPDPRVPSPACCLTIAGSDSGGGAGIQADLKTFAAAGVHG
                     ASVITALTAQNTRGVTAVHPVPAKHLRAELDAVFDDFAIAAVKTGMLGDAKTVRCVVR
                     ELARRKPRWLVVDPVMIATSGARLLDPDAVRVLIEGLVPLADVLTPNLPEAEALTGIA
                     IRGPREGQRAAERLLGFGAKAVLLKGGHGRGREAIDRWFDPRGVLEFRHPRLPGQFHG
                     TGCTLSSALAAELAKGATPRTAARRATAFVQHAMQGAFRPGDGELAVLRV"
     gene            complement(1483..2871)
                     /locus_tag="VFK00_02140"
     CDS             complement(1483..2871)
                     /locus_tag="VFK00_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7591788.1"
                     /translation="MAQQVKGKRTYALDTNVLLHDPTALFRFEEHDVFIPMTVLEELD
                     EKKQGISEVARNARQASRFLNELIERNNSHGIADGVPLAQPDGVRLRSGGASGRLYFQ
                     TRSGNGQGKADNRILAAILDFHEHRPDAAVALVSKDINLRIKATIFGIAAEDYENDRA
                     LDDFSLLYTGSLELPADFWERHPELRSWSERGHTFYELKPREDEAWQPNECLYLPGEN
                     SVELRVLESSGDKARLALLGDYTGSAHGVWGITARNREQNFALNMLMDPDIDFVTLLG
                     TAGTGKTLLALAAGLAQVMDQQRYREIITTRATVSVGEDIGFLPGTEEEKMTPWMGAL
                     TDNLEVLANPEEGGSWGRQATNDLLASRIKIRSLNFMRGRTFLSRYLIIDEAQNLTPK
                     QMKTLVTRAGPGTKIVCLGNVEQIDTPYLTETTSGLTYAVDRFKSWPHAAHITLRRGE
                     RSRLADFASETL"
     gene            complement(3003..3461)
                     /locus_tag="VFK00_02145"
     CDS             complement(3003..3461)
                     /locus_tag="VFK00_02145"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7591789.1"
                     /translation="MVKIGNKVPRLSGTLHSGDAFSLADLRDQWVVLYFYPKDNTTGC
                     TREAGDFRDLYTRFKRRGAAIVGVSRDSARSHSGFKEKLGLPFPLVADTDESWCKAFD
                     VIRKKKLYGREYLGVERSTFLIDPEGRLVREWRGVKVPGHAQAVLDAVAA"
     gene            complement(3583..>3812)
                     /locus_tag="VFK00_02150"
     CDS             complement(3583..>3812)
                     /locus_tag="VFK00_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7591790.1"
                     /translation="AGVLVELIDFFNRRKISIEQMSSMRYQAMQTGADMFQAQLTVGI
                     PAETRIAELRDEFMELCDGLNLDAIMDPVKF"
BASE COUNT          610 a         1203 c         1314 g          685 t
ORIGIN      
        1 gcgatgcggg ttgagggtgg cgccgggcgg aagcagggcg aacatcgcgg ccttcacgcg
       61 cgggatccgg ttcaccagtt ccaccgtctt cgggcagagc ttctgcgccg acggcagcgg
      121 ttcgccgtac cacttcacgt agaagcgctt ccatcccacc ttgatgaacg agctgaagct
      181 ggcgtcgtcg tcgttgagcg cggcgcggat gtggccctgg tcggaaagac ccgccgcctc
      241 atcgcggatg gtcttccagt tgtcgcgcag caggtccacg tcggggaaac cacgacggtc
      301 cgggaacgcg atgccgggga ccgcggaaaa cgcgtacatc aacaggttgt acggcgcgaa
      361 cacggtggcg tggctggcga gctgcttgcg cagcctcagc cgcaccctgc tgcggaagtg
      421 cacggccagc gcgctgagca ggaacagcac gatcagggcg aggatggcaa ggcggatggc
      481 gagcaacatg gcgcggagat ccaggggacg cgtcgatttt agcgcggagt cacacccgga
      541 ggaccgcgag ttcgccatcg ccggggcgaa atgccccctg catcgcgtgc tgcacgaagg
      601 cggtggcgcg gcgtgcggcg gtgcgcggcg tggcgccctt cgcgagttcc gcggcgaggg
      661 ctgaagacag ggtgcaaccg gtgccgtgga attgcccggg caggcgcgga tggcgaaact
      721 ccaggacgcc gcgcgggtcg aaccagcggt cgatcgcttc acgcccgcgg ccgtgtccgc
      781 ccttcaggag cacggccttc gcgccgaacc cgagcagccg ttccgccgca cgctggcctt
      841 cgcgcgggcc gcggatcgcg atgccggtca acgcttcggc ttccgggagg ttgggagtca
      901 acacgtctgc cagcggaacc aggccttcga tcaacacgcg caccgcgtcc ggatccagca
      961 ggcgggcgcc ggaagtcgcg atcatcacgg gatctacgac cagccaccgg ggtttgcgcc
     1021 gcgcgagttc gcgcacgaca caacggacgg tcttcgcgtc gcccagcatt ccggtcttga
     1081 ctgcggcgat cgcgaagtcg tcgaacaccg cgtcgagctc cgcacggagg tgcttcgccg
     1141 gcaccgggtg caccgccgtg acgccgcggg tgttctgcgc ggtcagcgcg gttatcacgc
     1201 ttgcgccatg cacgcccgcc gcggcaaagg tcttgagatc cgcctggatg cccgcgccgc
     1261 cgccactgtc cgagcccgca atggtgaggc agcaagcggg actcgggact cggggatctg
     1321 ggcgcggtgc agccatcatg ttgtctcagg gctaccgccc ctgtcgctca gagcggctgt
     1381 tgtggatagt caggccaggg aaggccgttc tgcccaggca tgggccacat tcttcatgct
     1441 ggcggattgg ttcggcgaag cagcgatcac ggacgatcgc acctataagg tttccgaagc
     1501 gaaatccgcc agccgcgaac gctcgccgcg gcgcagcgtg atgtgcgccg cgtgcggcca
     1561 gctcttgaag cggtccaccg cgtaggtgag gcccgaggtg gtttcggtca ggtacggcgt
     1621 gtcgatctgc tcgacgttgc cgaggcaaac gatcttggtg ccggggccgg cgcgggtgac
     1681 gagggtcttc atctgcttgg gcgtcaggtt ctgggcctcg tcgatgatga ggtaacggga
     1741 caggaaggta cgcccgcgca tgaagttcag cgagcggatc ttgatgcgcg aagccagcag
     1801 gtcgttggtc gcctgtcgtc cccacgagcc gccctcctcg ggattggcca gcacttccag
     1861 gttgtcggtg agtgcgccca tccacggcgt catcttttct tcttccgttc cggggaggaa
     1921 gccgatgtcc tcgccgacgg acaccgtcgc gcgggtggtg atgatctcgc ggtagcgctg
     1981 ctggtccatc acctgggcga ggcccgcggc gagcgccagc agggtcttgc cggtgccggc
     2041 ggtgccgagc agggtgacga aatcgatgtc cggatccatc agcatgttga gcgcgaagtt
     2101 ctgttcgcgg ttgcgcgcgg tgatgcccca cacgccgtgc gcgctgccgg tgtagtcgcc
     2161 gagcagcgcc agcctggcct tgtcgccgga ggactcgagc acgcgcagct cgacgctgtt
     2221 ctcgcccggc aggtacaggc attcgttggg ctgccaggcc tcgtcctcgc gcggcttcag
     2281 ttcgtagaag gtgtgcccgc gctcgctcca cgaacgcaac tccggatggc gctcccagaa
     2341 atccgcgggc aattccagcg agccggtgta cagcagcgag aagtcgtcca gcgcacggtc
     2401 gttctcgtag tcctcggcgg cgatcccgaa gatggtcgcc ttgatccgca ggttgatgtc
     2461 cttggacacc agtgccaccg cggcgtcggg acgatgctcg tggaagtcca ggatcgcggc
     2521 gaggatccgg ttgtcggcct tgccctggcc gttgccgctg cgggtctgga agtagagccg
     2581 cccgctcgcg ccgccgctgc gcaaccggac gccgtcgggt tgcgcgagcg gcaccccatc
     2641 ggcgatgccg tggctgttgt tgcgctcgat cagttcgttg aggaagcggc tggcctggcg
     2701 ggcattgcgc gcgacttccg agatgccctg tttcttctcg tcgagttcct ccaacaccgt
     2761 catcgggatg aacacgtcgt gttcctcgaa gcggaacagc gcggtcggat cgtgcagcaa
     2821 aacattggtg tcgagcgcat aggtgcgctt gcccttgacc tgttgggcca tcagtcggca
     2881 gcctcgggga gtttgaaaag ggagttggat acggatcgaa gcggatcgac ccgcgcgaga
     2941 ctcttttgat cagtgtgcat tccgccgatc cgggtcaaag gcgtcggttc cggtgcgcgc
     3001 cactaggcgg ctacggcatc caggacggct tgggcgtggc cgggcacctt gacgccccgc
     3061 cactcccgaa ccaaccggcc ctcgggatcg atcaggaagg tgctgcgctc gacgcccagg
     3121 tactcgcggc catacagctt cttcttgcgg atcacgtcga aagccttgca ccagctctcg
     3181 tcggtgtctg ccaccagcgg gaacggcagc cccagctttt ccttgaagcc ggaatgggaa
     3241 cgtgcactgt cgcgcgacac gccgacgatc gcggcaccgc ggcgcttgaa cctggtgtac
     3301 aggtcgcgga agtcgccggc ttcccgggtg cagccggtgg tgttgtcctt cggatagaag
     3361 tacagcacga cccattggtc ccgcaggtcg gcgagcgaga acgcatcgcc cgaatggagg
     3421 gtgccggaga gcctgggaac cttgttgccg attttgacca tcatggcgcc atgttgtctt
     3481 ctgcctcggc gtcattccgg cgcaggccgg aatccagctt ccactttcaa tccgtgggtc
     3541 tgggtcccgg ccagtccatg gccgggatga cgatgaatgc tcctagaact tgacgggatc
     3601 catgatcgcg tcgaggttga ggccgtcgca cagctccatg aactcgtccc gcagttcggc
     3661 gatccgtgtc tcggccggta tgccgaccgt gagttgggcc tggaacatgt cggcaccggt
     3721 ttgcatggcc tggtagcgca tcgaggacat ctgttcgatg ctgatcttgc ggcggttgaa
     3781 gaaatcgatc aactcgacca gtaccccggc ct
//
LOCUS       DASSNH010000062         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_199323, whole genome shotgun sequence.
ACCESSION   DASSNH010000062 DASSNH010000000
VERSION     DASSNH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199323"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFK00_02155"
     CDS             <1..247
                     /locus_tag="VFK00_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591791.1"
                     /translation="PIARIGGRSLGFASLSTNLQKHSDGACYTAFVGWVDPGLCPGEA
                     QQGVVNRLLESVGDRWASLRSAPTYKSIPTAPATQLS"
     gene            379..1461
                     /gene="mltG"
                     /locus_tag="VFK00_02160"
     CDS             379..1461
                     /gene="mltG"
                     /locus_tag="VFK00_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7591792.1"
                     /translation="MIRCDRCKGAASRRRQAGNVGLRLALAVILLAIAGAVAWGAFEY
                     LHFARTPLNVAAKGQTLEIARGEGFKDIVRQLRNERLTQASPLLWRALATRLGVVNRL
                     HAGEYALVPGTTPQALLEDMAAGRVLHRRVTLVDGWTFAQVRQALGKAPKLKHELAGL
                     DDAAVMAKLGDAGGSPEGRSPEGEFMPDTYDYVLGMSDMDILVRAHEAMQEFLDKEWP
                     ARDTSVPLDDPYQALILASLVEKETAVAAERPRIAGVFERRLKIGMKLDTDPSVIYGM
                     GKAYTGNIRKSDLETDTPYNTYTRAGLPPTPIALPSRAAIHAVLHPAPGDALYFVARG
                     DGTHQFSATLAEHNAAVRKYILGKKR"
     gene            1461..2093
                     /gene="tmk"
                     /locus_tag="VFK00_02165"
     CDS             1461..2093
                     /gene="tmk"
                     /locus_tag="VFK00_02165"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7591793.1"
                     /translation="MRGRMISLEGGEGAGKSTLLAGLREHFRKTAQNVLYAREPGGTA
                     LGEAIRTLVLDPAHRGITAEAELLLMFAARAQLVREVLRPALAAGHWVLCDRFTDASY
                     AYQGGGRGIEAARIAELERIATGGLAPDLTLLLDLPVAHGRARASQRGTADRIEAERD
                     DFFERVRSTYHARAQAEPGRFRVIDASLPPDEVLQSAIAAVEALRAKATA"
     gene            2090..3100
                     /gene="holB"
                     /locus_tag="VFK00_02170"
     CDS             2090..3100
                     /gene="holB"
                     /locus_tag="VFK00_02170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7591794.1"
                     /translation="MSKDDAAAAVPAIAPGDTLPPWCMPLWEGLANRAASDTLPHALL
                     LCGPPGLGKRALAETFVRARLCEAPRDGRACGSCRTCKLLAAGTHPDRMLVTLEVNEK
                     TGKLRKEIVIDQIRGLSAHLAMASQLGGWQVAVIDPADEMNPAAQNALLKTLEEPTQA
                     TLIVLVADRPWRLSATIRSRCQRIDFVVPRREEALAWLVSRRVEAAEPALAAAGGNPG
                     AAWQLATHGGMDRRQEVAKDLLALAAGRARAREVAKAWAGDEPEQRLEHAARLLEGTL
                     RARAAGGRARFDPGWDDGTLASGFARANRLRMLLNGPLRTDLALFDWLDGLAVAESSG
                     TR"
     gene            3230..4207
                     /locus_tag="VFK00_02175"
     CDS             3230..4207
                     /locus_tag="VFK00_02175"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7591795.1"
                     /translation="MEKPSDLLDPETLLDRPNASLMVLAPHPDDESLAAGGLVQRALS
                     CGARVSVVFVTDGENNPWPQRALERRLWIGAHQREGWARRRRAEADQALRELGAESAS
                     VRRLGWPDGGVTWMLLEETGAMLATMRGLLDEEQPTVLVLPDLVDRHPDHSAMHVLME
                     MAFQSMPRASKPECLGYLLHGRSRAGAPLRAVFTLHPDELARKRRAIAAHTSQTALSG
                     RRLMRSATDTESFVAGLDSHDRNRPTLPWLPPRPVRRWLSLLAVDRNGGEKVPLSTRD
                     ANLFWRDGSPAARTSRPLQPPYYLKLYCPLPSPWVFDGWGWCRFGAPRA"
     gene            4293..4634
                     /locus_tag="VFK00_02180"
     CDS             4293..4634
                     /locus_tag="VFK00_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7591796.1"
                     /translation="MGSQGILSVVIKDKHTLYQSYMSFVKGGGLFVPTNKRYNLGDEV
                     FLLMTLPEETERVATTGRVIWITPIAAQGNRPAGIGVQFAENADGDNARTKIETLLAG
                     MQNSDKPTHTM"
     gene            complement(4862..4938)
                     /locus_tag="VFK00_02185"
     tRNA            complement(4862..4938)
                     /locus_tag="VFK00_02185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4902..4904),aa:Pro,seq:cgg)
     gene            5057..>5658
                     /locus_tag="VFK00_02190"
     CDS             5057..>5658
                     /locus_tag="VFK00_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET7591797.1"
                     /translation="MALTTPRTMPGVLELLPREQIAFQRMLDTIRHGFEVYGFLPIET
                     PAIEFADILLTKGGGETERQVYFVQSTGALEQSGKPEHALRFDLTVPLARYVAEHERD
                     LAFPFRRYQMQRVYRGERAQRGRFREFYQCDIDVIGKDSLSIRYDAEIPAVIHAVFRA
                     LDIGAFTIWLNNRKLMRGFFEGLGISDGGQQMLVLREVDKL"
BASE COUNT          960 a         1902 c         1945 g          851 t
ORIGIN      
        1 accgattgct cgaatcggtg ggcgatcgtt gggcttcgct tcgctcagca ccaacctaca
       61 aaagcattcc gacggcgcct gctacacagc tttcgtaggt tgggttgatc ccggactctg
      121 tccgggagaa gcccaacagg gcgtggtcaa ccgattgctc gaatcggtgg gcgatcgttg
      181 ggcttcgctg cgctcagcac caacctacaa aagcattccg acggcgcctg ctacgcagct
      241 ttcgtaggtt ggattgatcc cggactctgt tcgcgagaag cccaacgcat gttggttacc
      301 gttgggcttc gctgcgctcg gcaccgacct gcaaaagcca cgcccgtcga atcggcgcat
      361 atcgtttaga ctgcccggat gattcgatgc gatcgatgca agggcgcagc atcgcgccgg
      421 cggcaggcgg gcaacgtggg cctgcggctc gcgctggccg tgatcctgtt ggctatcgcg
      481 ggggccgttg cgtggggcgc cttcgaatac ctgcacttcg cacgcacgcc gctgaacgtc
      541 gcggccaagg gccagacgct ggaaatagcc cgcggcgaag gcttcaagga catcgtgcgg
      601 caattgcgca atgaaaggct gacccaggcg tcgccgctgc tgtggcgcgc cctcgcgacc
      661 cgcctcggcg tggtcaaccg gctgcacgcc ggcgagtacg cgctcgtccc cggaaccacg
      721 ccgcaggcac tgctcgagga catggccgcc gggcgtgtcc tgcaccggcg ggtcacgctg
      781 gtggacggct ggacattcgc gcaggttcgg caggccctcg gcaaggcgcc gaagctgaag
      841 cacgaactcg cagggctcga cgatgccgcg gtgatggcga agctcggcga cgccggcggc
      901 tcgccggaag gccggtcgcc cgaaggcgag ttcatgcccg acacctacga ttacgtactc
      961 ggcatgtccg acatggacat cctggtgcgc gcgcacgagg cgatgcagga attcctggac
     1021 aaggaatggc ccgcgcgcga cacgtcggtg ccgctcgacg atccctacca ggcgctgatc
     1081 ctcgcctcgc tggtcgagaa ggaaaccgcg gtagcggcgg aacggccgag gatcgctggc
     1141 gtgttcgagc gccgcctcaa gatcggcatg aaactcgaca ccgatcccag cgtgatctac
     1201 ggcatgggca aggcctacac cggcaacatc cgcaagagcg acctcgaaac cgacacgccc
     1261 tacaacacct acacgcgcgc cgggttgcca ccgacgccga tcgcattgcc cagccgcgcg
     1321 gcgatccacg cggtgctgca tccggccccg ggcgacgcgc tgtatttcgt cgccaggggc
     1381 gacggcaccc accagttctc ggcgacactc gccgagcaca acgcggcggt gcggaaatac
     1441 atcctcggga agaagcgctg atgcgcgggc gcatgatctc gctcgaaggc ggcgagggcg
     1501 cgggcaagag cacgctcctg gccgggctcc gcgaacattt ccggaaaacc gcacaaaacg
     1561 tgttgtatgc acgcgagccc ggcggcaccg cattgggcga agccatccgc acgctggtgc
     1621 tcgacccggc gcaccgtggc atcacggccg aagccgaatt gctgctgatg ttcgcggcgc
     1681 gggcgcaact cgtgcgcgag gtattgcggc ccgcgctggc ggcgggacat tgggtgctgt
     1741 gcgaccgctt caccgacgcg agttatgcct accagggtgg cggtcgcggc atcgaggccg
     1801 cgcggatcgc cgagctggag cgcatcgcca ccggtggcct cgcgcccgac ctgaccctgc
     1861 tgctcgacct gccggtggcg catgggcgcg cccgcgcgag ccagcgcggc acggccgatc
     1921 gcatcgaagc cgagcgggac gattttttcg agcgcgtgcg cagcacctat cacgcccgcg
     1981 cgcaggccga gcccgggcgc ttccgggtga tcgacgcgtc actgcctccc gacgaagtgc
     2041 tgcagtccgc gatcgccgcg gtcgaagcat tgcgtgcgaa ggcaaccgca tgagcaagga
     2101 cgacgcagcc gctgcggtac ccgcgatcgc acccggcgat acattgccgc catggtgcat
     2161 gccgctgtgg gaaggcctcg cgaatcgcgc ggcctcggac accttgcccc atgcattgct
     2221 cctgtgcggg ccgccgggac tcggcaagcg cgcgctcgcc gaaacattcg tgcgcgcccg
     2281 tttgtgcgaa gcgccacgcg acggccgggc atgcgggtcg tgccgcacct gcaagctgct
     2341 ggcggcgggt acccatcccg accggatgct ggtgacgctc gaggtcaacg agaaaaccgg
     2401 caagctgcgc aaggaaatcg tgatcgacca gatccgtggc ttgtccgcgc acctggcgat
     2461 ggcgagccaa ctcggcggct ggcaggtggc cgtgatcgat ccggcggacg agatgaaccc
     2521 ggccgcccag aacgcgctgc tgaaaacgct cgaagaaccc acgcaggcca ccctgatcgt
     2581 gctggttgcc gatcgcccct ggcgattgag cgcgacgatc cggagccgct gccagcgcat
     2641 cgacttcgtg gtcccgcgcc gcgaagaagc gctggcttgg ctggtgtcgc gtagggtgga
     2701 agcagccgag ccggcattgg ctgcggccgg cggcaatccg ggtgctgcgt ggcaattggc
     2761 cacgcacggc gggatggaca ggcgacagga ggttgcgaag gacctgctgg cgctggcggc
     2821 gggacgagcc cgcgcccgcg aggtcgcgaa ggcgtgggcg ggcgacgaac cggaacagcg
     2881 cctggagcac gcggcacggt tgctggaagg gaccttgcgc gcgcgtgcgg cgggtgggcg
     2941 tgcgcgcttc gatccgggct gggacgacgg gacgctcgca agcggcttcg cacgcgccaa
     3001 ccgcctgcgc atgctgctga acggacccct gcgaaccgac ctcgcgctct tcgactggct
     3061 ggacggcctg gcggtggcgg agtcatccgg cacccggtga tagccgcgtg tgggggcagt
     3121 ggcgccgcgc tgcctgcggt cagtgcgcgg tcaagcgcgc ccggtcacaa tcgcgcccca
     3181 tcctcgcgcc atgacccagg gcgcgtgacc caaggaatgc gaattgcgcg tggaaaagcc
     3241 atcggatctg ctcgaccccg aaacgctgct ggatcgcccg aatgccagcc tgatggtgct
     3301 ggcgccgcac ccggacgatg aatcgctggc cgcgggcggc ctggtccagc gggcgctcag
     3361 ctgcggcgcc cgggtgagcg tggtgtttgt caccgacggc gagaacaacc cgtggccgca
     3421 gcgagcactg gaacggcgcc tgtggatcgg ggcacaccag cgggagggct gggcgcgccg
     3481 ccgccgcgcg gaggccgacc aggcgcttcg cgagctcggc gcggaatcgg caagcgtgcg
     3541 ccggctgggc tggcccgacg gcggcgtcac gtggatgctg ctcgaggaaa caggcgccat
     3601 gctggccacg atgcgcggac tgctggatga ggaacagccg acggtcctcg tgctgccgga
     3661 cctcgtcgac cgccatcccg accacagcgc gatgcacgtg ctgatggaaa tggcattcca
     3721 gagcatgccc cgggcgtcga aaccggagtg cctcggctac ctgctgcacg gccgctcgcg
     3781 ggcgggagcc cctctccgcg ccgtattcac gctgcatccg gacgagttgg cacgcaagcg
     3841 ccgggccatc gccgcacaca ccagccagac cgcgctcagc ggccgccgac tgatgcgctc
     3901 ggcgaccgat accgagtcct tcgtcgcagg cctcgattcc catgatcgca accggccaac
     3961 cctgccatgg ttgccgccgc gcccggttag gcgatggttg tcgctgctgg cagtggaccg
     4021 gaacggtggc gaaaaggtgc ccctgtccac ccgggatgcg aacctgttct ggcgggacgg
     4081 cagtccggcc gcacgcacct cgcgtcccct gcagccgccg tattacctaa agttgtactg
     4141 cccgctgccg tcaccgtggg tattcgatgg gtggggctgg tgccgttttg gcgcgccgcg
     4201 tgcttgaccg ccatggggcc aaggcggcaa gctgtcgcac cctgatcgac gaggttttac
     4261 ggaatatggc agcaaaggcc ggcgcgggcg ggatggggag tcagggcatc ctgtccgtgg
     4321 tcatcaagga caagcacacc ctctaccagt cctacatgtc gttcgtgaag ggcggcggcc
     4381 tgttcgtgcc caccaacaag cgctacaacc tcggcgacga agtattcctg ctgatgacgc
     4441 tgccggagga aaccgaacgc gtggcgacca ccggccgggt catctggatc accccgatcg
     4501 cggcgcaggg caaccgcccc gccggcatcg gcgtccagtt cgccgaaaac gccgatggcg
     4561 acaacgcccg caccaagatc gaaaccctgc tcgccggcat gcagaacagc gacaagccca
     4621 cgcacacgat gtgaggggcg ctccggtcgc gcgctaccgg cggtcacgga cgcgcggctc
     4681 atccccccgg ccggaacagt ttcgcgacgc cctgtgggag cgggtcatgc ccgcgaccgg
     4741 ggcggttttg ctgttatgta ggagcgggct cgcccgcgac cggcggagag ctctcgacac
     4801 agtaccaacc atcgcaagca gcccactggg ctgcctggga aggcctgcat tcgcaaagac
     4861 ttggtcgggg agacaggatt tgaacctgcg acttctacgt cccgaacgta gcgctctacc
     4921 aggctgagct acaccccgac ggctcgcacg ggcgcggggc ccgggagcga gccgccaatg
     4981 atacttggcg ccgcttcgcg cgcgcaagaa gggcgcccgc gctgctaacc ttgcgctttt
     5041 atcacgagac gcaacgatgg cgctgacgac gccccgcacc atgcccggcg tgctggaact
     5101 gctgccgcgc gagcagatcg cgttccagcg gatgctggac acgatccggc acggtttcga
     5161 ggtgtacgga ttcctgccga tcgaaacgcc cgcgatcgag ttcgcggaca tcctgctgac
     5221 caagggcggc ggcgaaaccg agcggcaggt gtatttcgtg cagtcgaccg gtgcgctcga
     5281 acagtcgggc aaaccggagc acgcgctgcg cttcgacctg acggttccgc tggcgcgcta
     5341 tgtcgccgag cacgaacgcg atctcgcgtt ccccttccga cgctaccaga tgcagcgggt
     5401 gtaccgcggc gagcgcgccc agcgcggccg cttccgcgag ttctaccagt gcgacatcga
     5461 cgtgatcggc aaggattcgc tgtcgatccg ctacgacgcc gaaatcccgg cggtgatcca
     5521 cgcggtgttc cgcgcgctgg atatcggcgc gttcacgatc tggctcaaca accgcaagct
     5581 gatgcgcggc ttcttcgagg gcctcggcat ctcggacggc gggcaacaga tgctggtgct
     5641 gcgcgaggtg gacaagct
//
LOCUS       DASSNH010000063        21723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_376125, whole genome shotgun sequence.
ACCESSION   DASSNH010000063 DASSNH010000000
VERSION     DASSNH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 21723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21723
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376125"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFK00_02195"
     CDS             <1..1195
                     /locus_tag="VFK00_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7591798.1"
                     /translation="KVIMDLVANHSSNRSPWFEAARDPRSRYHDWYTWATPATDLKAI
                     SATGGPAWHSVPGGGHYLGVFTGEMPDLNYDDPALRKQMIEVGKFWLAKGVDGFRLDA
                     AQHVYLDFETDWGSLAALKENLAWWREFHRGIDTVDPQAWLVGEVWPRNPDQLASWMG
                     SLDAVFNFPLAERLVASAQQERDRDIGATLERVEQAYRAAAHGRFDDAPFLSNHDQER
                     VMSQLDGNPDHMRIAAAMLLTLPGHPFVYYGEELGMRGEKPDPHLREPMRWYRAGQGP
                     GEPTWEGWTAGDGPAISVEAEQADPGSLLNWYRMLIGWRRDIPALRDGVARNWRDASL
                     QVAAWELDDPHGDVLVLHNLSGEPQSVHLQGRRFHKLLRHSRADTQWADGRLRLPPYG
                     SAVLQ"
     gene            1222..3591
                     /locus_tag="VFK00_02200"
     CDS             1222..3591
                     /locus_tag="VFK00_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET7591799.1"
                     /translation="MKDLLRLLAFMMLAGPGLAVVARAPSENGVAKLRSVNAQGFELA
                     LGDATVSVQLRAPGVLHVHYVPDDRSTPPGTVIDPEREPAPAFAPGVLRKRDTVTLRS
                     ERMTVTWDGATHSLSVADAEGRPLLKQTDLAGLADGSIVLQHDAADPLYGIGGYTIHE
                     SVAPGLLRTGKQVAEAGKQGHAGAPLVWSTAGYGLLVDCEGATFDLAGGSITVGDYKR
                     PDTDYYILAGTPAEVLGEVSRISGPAPLFPKWAMGFTNSQWGIDQKELLDIVKTYRAK
                     HIPIDNFTLDFDWKAWGENNYGEFRWNPEKFPDGPSGKLAKTLDGQGIHLTGIMKPRI
                     HVNTVEGRYATAHKLWIPGEKPFEDYFSHKLVKRLDFDKPETRRWFGEAALKYAFDKG
                     IVGWWNDEADEIGSDTEFLNMERSLYDAQRAASDQRVWSLNRNFWLGAQRYAYGLWSG
                     DIDTGFESMAAQRTRMLSAIEVGATDWGMDGGGFNGHPSDENFARWIEFGAFTPIFRV
                     HGTMDEKRQPWRYGPIAEAAATRAIRLRYSLIPYIYAYQHAAHASGVGLVRPLTFDWP
                     HDPKVRNDVDAWLFGQWLLVAPVVEKGQTSKSIYLPAGTWTDWFTGKVHQGARTIDYK
                     VDAKTWRDIPLFIRAGAIIPTQPVMDYVGQHPSKTVTIEVFPADHATHFDYYDDDGET
                     YAYEKGVYFLQRLTAGRDGSVVSFRADAPMGSYRPALRDYLVRIHGTAAASVQTGGAE
                     LRRYADAKALARAQGQGWTTGHDQYGAVTIVKLDAGVVRDVTLRGSAQQ"
     gene            3813..5429
                     /locus_tag="VFK00_02205"
     CDS             3813..5429
                     /locus_tag="VFK00_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HET7591800.1"
                     /translation="MKKIAKRVRCFALGIAAFALFAAPGLAVAAAAPAPQTIQLKVDA
                     RASGTPFPHFWEQMFGSGRAVLSLRDDYRKDLDAVHQATGFTYVRFHGIFDRDVGLVQ
                     RGQDGRISYNFSYVDQIYNGLLAHGMKPFVELGFMPPDLSSDPSNHHSFWYHPNVMPP
                     KNYAEWDAMIRAFAQHLIDRYGIDEVASWYFEVWNEPNIDFWGGEPKQSTYFELYDHT
                     ARTLKSVSDRLRVGGPATAQAAWVPAFLAHVHEAGIPIDFVSSHVYGDDTAENVFHTD
                     EHIPRKDMVCRAVAKVHDEILASPYPKLPLVFSEYNASYANLPNVTDTVFMGPWLANT
                     IRACAGKVAMMSYWTFSDVFEEQGVVRTPFYGGFGLIAEDRIDKPAFNAFAMLHKLGG
                     ERLPLSSDSALATRRPDGSLVLALWNYAPPVGSGATYMPGPPQGSPKHFEITFQHLAA
                     PATAAVWRLDSMHGNVIATFDKMRRPAFPSRTQIEQLRAAGRLGDPQQVGVHDGRLTF
                     DIPPQGLVVVEIPSTNTRHANAAEKPGAGT"
     gene            5447..7609
                     /locus_tag="VFK00_02210"
     CDS             5447..7609
                     /locus_tag="VFK00_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Six-hairpin glycosidase-like protein"
                     /protein_id="HET7591801.1"
                     /translation="MLATTAMQAAPVWHASLEWRGASASMSAQADGSYALTGPFGERR
                     IPAPPAKAQTASVLFDGLFAMAQEELAQDSVSVLHDPAYDHGGAMDCRCFVTGVKWPY
                     VWTRDVSYSIDLGLWRYDPARARESLLFKLSDVRAKDVPQGSYPMQDTGSGGSWPIST
                     DRVAWFLGARHLLDDRAFADKTWRALNDTLAQDRAYAFDPGLGLYRGETSFLDWREQT
                     YPAWTKDNVVFIGQSFALSTNVLHYEALRLAARMANERHDPRGAEYTAHADALKQAIN
                     AHFWRDDRGMYMSYIGGDGMPVEAYDLLGLSLAITSGVADEARARSSLSHYPVWPAGS
                     PVIWPERADQPIYHNRAIWPFVSAYALRAARKIDDPALIALEIRSMIRGPALAGSNME
                     NYAMLSQATHVDEGKLSGPVVNSPRQLWSVAGYLDMVAEGVFGLTADGKVEPKLPVSL
                     VSTLFGDRGEISLQLPHRQITLRRPDRLDGNLLVADKVETHGDRTVVMLKALEVPGLN
                     LRTDAPLYAPATPAAPDARRDGDTWNVRSIEDGDHVLYVDGKRHGSAGDAWTLRASDL
                     RYCLRLTRLGEHGIESLPSEATCMGDDARVTGSWPRAWTAPRGGRYRVWLDYTNDHGP
                     INTGITVGVKRLAIDCTGSPAQTVTIVMPHSVAEQRSTAATFETKAGARCTFALEQGF
                     NMSDLAHFVHYTGGGGGASGPLNEANVNALHVAPLPSQ"
     gene            7839..9341
                     /locus_tag="VFK00_02215"
     CDS             7839..9341
                     /locus_tag="VFK00_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7591802.1"
                     /translation="MTRKPALSFWQIWNMCFGFLGIQFGFALQNANVSRIFQTLGAGV
                     DAIPALWIAAPLTGLIVQPLIGHWSDRTWGRLGRRRPYFLVGAVLATLALLVMPNAPL
                     LWVAAGLLWIMDASFNVAMEPFRAFVGDQLPTQQRARGYAMQSFFIGVGAVVASLLPW
                     LLARFGVPNTAPEGAIPDTVKYAFYAGGAVLLGSVLWTVFTTREYPPDALLAFDDSAE
                     AAPTSDVSRAWRIGAALGTLGVLLLALILRFSLARDLYLLAGMLIAAGVLFAWLSRTR
                     SAGTVRQVMGDLYRMPEAMRRLAWVQFFSWFALFAMWIYTTPAVTAVQFGATDPHSAA
                     YNTGANWVGVLFAAYNGFAALAAILIPWMARAWGLRVSHLVNVTLGGVGLISFALIRD
                     PHWLLLSMLGVGFAWASILSLPYALLSDNLPAAKMGVYMGIFNFFIVIPQLLAASVLG
                     VLLKWFFHDQPLYALVLGGASLIVAGLCTLRVREPDAPVSAGAGAAASPG"
     gene            9446..9685
                     /locus_tag="VFK00_02220"
     CDS             9446..9685
                     /locus_tag="VFK00_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591803.1"
                     /translation="MEFHIKTLGARPDLGAINDALIVADPMAMADLDPAGGTLRVAAE
                     LSSPELLSLLTRSGYPVDWRQLEQVPSVCCGACGG"
     gene            9814..11115
                     /locus_tag="VFK00_02225"
     CDS             9814..11115
                     /locus_tag="VFK00_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7591804.1"
                     /translation="MDDDVRGPTRARLTEHTRAEIRAGLEGRRRGLALLLPFAGPAVV
                     VSTAYLDPGNFAVNIQAGARYGFSLVWVVLLASLIAMLFQALAAKLGIVTGRNLAQLC
                     RERMPRPLAMSMWAVSEAAAMATDLAEFVGGAIGVSLLLQIPMMAGMIITAIATCALL
                     LLERSGFRPLELAIGSFVVVIGLCYLAELVIQPVAWPGLLASQWPPRFADSGAVLLAC
                     GIVGSTVMPHALFLHSGLVQRRATPRTPQQRSTLLRFARIEAVVALGIAAVVNMAMVV
                     MAADAFYPARAQDAVLQSAYHTLGPLLGPAAAGVFLVALIASGISSSVVGTLAGQMVM
                     QGFAGFPLSVWLRRAVTMVPSFVVVALGIDATRALVLSQVVLSLALPVPVAALLWFTG
                     RRDLMGRYRNGLPTRVVAAVAAGAVVALNIVLLVESAASNP"
     gene            11401..11811
                     /locus_tag="VFK00_02230"
     CDS             11401..11811
                     /locus_tag="VFK00_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7591805.1"
                     /translation="MIQQQTVSLDDARRVIAAGEDKAREIGQPMNIAVVDDGGNLVAF
                     ARMDGAWKGSVDIAVNKAFTSAMFTIPTKALTKKAQPGEPFYGIHVSNHGRVMIFPGG
                     VPLKAGDQVIGAVGVSGGDNSQDQGVAEAAADAL"
     gene            11939..12457
                     /locus_tag="VFK00_02235"
     CDS             11939..12457
                     /locus_tag="VFK00_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7591806.1"
                     /translation="MRKSIFLLVFVLGLPLAAGAAQSPQSSSQDRSWLIAAHQDNLAE
                     IESGELAAAKGHDADVRAAARTLTRDHGDLDAKLRPVAKQLGAKLPTQPNAEQRDEMR
                     KFKSASGVDFDRTWTHDEADAHVKAIELTVREIQHGSLPQVKQLAQSALPVLKKHLHM
                     LQQTQSARSGNQ"
     gene            12511..14370
                     /locus_tag="VFK00_02240"
     CDS             12511..14370
                     /locus_tag="VFK00_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HET7591807.1"
                     /translation="MKMLALATAVSAALLATSMARAAVHPAKPASAGNAAAQSATGES
                     VTQLASNPADWPMPGKNYAGTRFSGLKQIDANNVRNLSVAWTFSTGVLRGHEAAPIVV
                     GDTMYVVTPWPDILYALDLSKDGAIKWVFKPYPSRASQGVACCDVVNRGVAYSDGKIF
                     MNTLDDHTIAVDAKTGKQVWNTKIGDINHGETITMAPLVVKNHVLVGVSGGELGVRGW
                     LKALDTNTGQVQWTAYSTGPDKDVLIGKDFNPPYPEDKGPNLGETSWPNKAAWMIGGG
                     PVWGWITYDPKQDLIYYGTGNPGPWNAYQRPGLNFWTCTLFARDPDTGMAKWAVQWNP
                     HDTHDYDGVNENVLFNATIDGKERELLAHPDRNGFMYVQDRTTGKIISAQPFMPGTNT
                     IERIDLVTGAPVVNPEKVPQLDKTVHDICPASTGGKDWQPSAYSPMTGLLYIPHNHLC
                     QDEEVSKVSYIEGTPFVGAKVAMKAAPGGWRGAFTAYDPVHAKVVWSDHENFPVWSGA
                     LVTAGGVAFYGTMDRWFKAVDAKTGKLLWKFRVGSGISGQPIAFKGPDGREYVAVLAG
                     VGGWSGAVVAGDLDPRNPYGALGFVGATQDLPDYTNKGGTLYVFALPKPGGSP"
     gene            14379..15260
                     /locus_tag="VFK00_02245"
     CDS             14379..15260
                     /locus_tag="VFK00_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HET7591808.1"
                     /translation="MLLLLALAAAPAFAFAQTHTQYPGAAPATASPSLRVCADPNNLP
                     FSNEAEEGFENKLARMLAHSLGKQAQFVWHEQGKEFLRDTLNAGICDVVIGLPTDDHA
                     YGVLTTDPYYRSAYVLVYRADAPYQLKSLDDPRLRTLEIGVHATGDDWVDLPGGATLA
                     HRGIVKNVHVYTLGVDDSHANPSADLVDAVAKGDIDVAIAWGPLAGYFASREPAKLKL
                     VPLTDTHTLLPFQFSISMAVRHGNGALRDKLNAFLATHRAAVGQLLARYGVPQLNIPA
                     AGAPRLQAGFARSGASE"
     gene            15286..15912
                     /locus_tag="VFK00_02250"
     CDS             15286..15912
                     /locus_tag="VFK00_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7591809.1"
                     /translation="MKTVWVALVLAFALAATAFGFAQVASPRVQASPAQQLENTPVSD
                     LHIGVPAQMKNPYAGDRDAWMQGKKLFKAMNCAGCHAPGGGGGMGPPLSDSHWIYGGL
                     PGQIYLSIEHGRPNGMPQWGTVLPSKAIWELVTYVETLPQPNPEFEPQTKLGVKPMPG
                     NTPHPATQRVPPRGPKHQKPTPKPAYPAPASTPAKAPPAAPASTTGKH"
     gene            15912..16925
                     /gene="coxB"
                     /locus_tag="VFK00_02255"
     CDS             15912..16925
                     /gene="coxB"
                     /locus_tag="VFK00_02255"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7591810.1"
                     /translation="MRRLPAILVMLAPALAHAGPMTFMVSHGAAANPITKLGWGLLAL
                     SIFVGAFIALAVLIGIWWKRGAGEYDERGWPVLSHGRNGLPWLWIGVPVSIVLLLLSA
                     IWTFYVLGVATAAPGHPKITVEVIGHQWWWEVRYSEFGYPRLSFDTANEIHIPVGVPV
                     EVHLTSADVIHSFWVPQLAGKTDTIPGQINHAWLEADKPGVYRGQCTEFCGLQHAHMA
                     LYVVAQPARQFKAWWKHQLQPAADQVTGSAGEGEDVFRTRCAICHTARGMPGSQGKLG
                     PDLTHLMGRRTIAAGTLRNTPGNLAAWVSDAPAQKPGVDMPAEDLTGPQLQALLAYLK
                     TLK"
     gene            17022..18962
                     /locus_tag="VFK00_02260"
     CDS             17022..18962
                     /locus_tag="VFK00_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7591811.1"
                     /translation="MAANPTFQTPEIVVADEELRHKLEQMWSDPPGIYGWFATTDHKK
                     IGKRYLFTAFVFLIVGGLEALGMRLQLAQPNEHLLSPELYNQFFSMHGFTMIFLYALP
                     ILSGFSNYLWPLILGTRDMAFPRLNAFTYWVFLLSGIFLYIGFWNGKGTDVGWFNYVP
                     LAERAYNPDWNVDIYSLGMIFLGISTTAGAVNFIATMFRTRAPGMSISRVPIMIWGTL
                     VSNIGILFAVPAVSLDFLMLWLDRRFGTQFFNSSAGGRPLLWQHLFWIFGHPWVYAIV
                     LPAMSIVSQGLPVYCRRPLVAHSAVVLATILTMVLGFGVWVHHMFSTGLPVIALGFFA
                     AASFVIVIPSAVSVFAWVATIWTGRPVFTTAFKFFAAEIVLFVIGGVSGFMTAAVPLD
                     WQLTDTYFVVAHIHYVLIGINVFPVIGGIYHWFPKMTGRMMNERLGWWNFWLMFIGFN
                     LGFFPMHISGLEGMPRRIYTYPANLGWGDLNLVTTIGAFVFALGVLLFVINVVRSRFA
                     GAPAPANPWDASTLEWATTSPPPSHNFTVIPQVASLDPLWEERLEGGRQSLITEGPVL
                     DDEHEIPGTTVLDAEPDVIMKMPHATLAPISLALLIAGTFVGLLMSMWWVAAGCLLAM
                     IPALLWWFWPSPEMGFAERVRP"
     gene            18959..19570
                     /locus_tag="VFK00_02265"
     CDS             18959..19570
                     /locus_tag="VFK00_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7591812.1"
                     /translation="MTAYDERTLPRPLPIGPAGWRTPGWWGVLALIGTEAAVFAYLLF
                     SYFYLQSQTTEAWPPSGLPPLRWSAPSTAVMLISVATTWWADHGMRLGSIGRLYSGLA
                     ATSLLGICYIILQCFDWRGEPFLANSGAYGSLFYAITAFHVLHAAAGVLILLAVLGWS
                     VMGYLTPRRNSAVTVVAMYWYFVVVAWIAVFVSLYLLPWISTH"
     gene            19579..19992
                     /locus_tag="VFK00_02270"
     CDS             19579..19992
                     /locus_tag="VFK00_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591813.1"
                     /translation="MRAVTRPNPHVPARIPVGWALTFAVFGAPLAWMLQLCFGYALAA
                     HACFPIDVPLVTPVWPSLWWVLVGIDLVALAIAGTGLFTAWSCHLAWRGVDPRDVGER
                     RNRFIAQWSVLTSALFSIALIFTIVMVFIEPVCNY"
     gene            20164..21369
                     /locus_tag="VFK00_02275"
     CDS             20164..21369
                     /locus_tag="VFK00_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7591814.1"
                     /translation="MSHLWTEWSWEPWLLVCLAAAVVPYVAGMLRMGARRTQILGRWR
                     APSFFGGLLLLFLALMSPLDEVADSLFSAHMLQHMLLLLVIPPLLVYGRPVITWLWAF
                     DTDSRRAVVRGWKWSKLEAMFAFLMRPLCVWTLLTAALCYWHLPGPYDAAVRHEWLHD
                     LEHLSFLAFALAFWTIVIEPYGRRRALGYGATAVFVVSSGFVMSLIGAILTFATTPLY
                     AVHLQTTQAYGLTPLEDQQLAGIIMWIPSNLVHVGALSAVFFAWFRADERRARERVAP
                     ARATSRTALHTLLVLPLVAGMLGGCAHRPSPDQVEARRGAHLIEHYGCGSCHMIPGIN
                     GADGLVGPPLIHWSRRTYIAGVLPNDPDNLAFWVQHPQKVIPGVDMPDMGLKQRDAQA
                     IAAYLDTIQ"
     gene            21515..>21723
                     /locus_tag="VFK00_02280"
     CDS             21515..>21723
                     /locus_tag="VFK00_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7591815.1"
                     /translation="MRAVTFHGKEDFRVDNVPDPRIEQPTDAIIRITSTAICGSDLHL
                     YSVLTMFLEEGDIIGHEPMGIVEEVG"
BASE COUNT         3603 a         7002 c         7259 g         3859 t
ORIGIN      
        1 caaggtgatc atggacctgg tcgccaacca ttccagcaac cggtcgccgt ggttcgaggc
       61 cgcacgcgat ccgcgcagcc gctaccacga ctggtacacg tgggcgacgc ccgcgaccga
      121 cctgaaagcg atcagcgcca ccggcgggcc ggcgtggcac tcggtgcccg gcggcggcca
      181 ctacctcggc gtattcactg gcgaaatgcc cgacctcaat tacgacgatc cggccctccg
      241 caaacagatg atcgaggtcg ggaaattctg gttggccaag ggcgtcgacg gctttcggct
      301 cgatgcagcg cagcatgtct acctcgattt cgaaaccgat tggggtagcc tggccgcgct
      361 gaaggagaac ctggcgtggt ggcgcgagtt ccatcgcggc atcgacacgg tcgatcccca
      421 ggcatggttg gtgggcgagg tatggccgcg caacccggac caactcgcgt cgtggatggg
      481 ttcgctggac gcggtgttca atttcccgct cgccgagcgc ctggtcgcaa gcgcgcagca
      541 ggaacgcgat cgcgacatcg gcgccacgct ggaacgcgtc gagcaggcct atcgcgctgc
      601 cgcgcacggc cgtttcgacg acgcgccgtt cctgtcgaac cacgaccagg aacgggtgat
      661 gagccaactc gatggcaatc cggaccacat gcggattgcg gcggcgatgc tgctgacgct
      721 gccgggtcat ccgttcgtct actacggcga ggaactcggg atgcgcggtg aaaagcccga
      781 tccgcacctg cgcgaaccga tgcgctggta ccgcgcgggc cagggtccgg gcgagccgac
      841 gtgggagggc tggacggccg gcgacggacc cgcgatctcc gtcgaagcgg aacaggccga
      901 ccccggctcg ctgctcaact ggtaccggat gctgatcggc tggcggcgcg acattcccgc
      961 cctgcgtgac ggggtcgcgc gcaactggcg tgatgccagc ctgcaggttg cggcgtggga
     1021 actcgacgat ccgcacggcg acgtgctggt gctgcacaat ctgtcgggcg aaccgcaatc
     1081 ggtccatctg caggggcggc gcttccacaa actgctgcgg cacagtcgcg cggacaccca
     1141 atgggccgat ggccggctgc ggttgccgcc ctacggcagc gcggtattgc aatgaaccaa
     1201 agatttttcg ggggactgcg aatgaaggat ttgttgcgcc tgttggcgtt catgatgctg
     1261 gccgggcccg gcctcgccgt ggtggcccgc gcgccgtccg aaaacggggt cgcgaagctg
     1321 cgcagcgtca acgcccaggg gttcgagctc gcgctgggtg acgcaaccgt ctcggtgcaa
     1381 ctgagggcgc cgggtgtgct gcacgtgcac tacgtgccgg atgatcgcag cacgccgcca
     1441 ggcacggtga tcgatccgga gcgtgaacca gcgccggcgt tcgcacccgg ggttttgcgc
     1501 aagcgcgaca cggttacgct gcgttccgag cgcatgaccg tgacctggga tggcgcgacg
     1561 cacagcttga gtgttgccga cgccgaaggc cgcccgctgc tgaaacagac cgacctggcc
     1621 gggttggccg acggcagcat cgtgctgcag catgacgccg ccgacccgct gtacggcatc
     1681 ggcggttaca cgatccacga aagcgttgcc ccgggcctgc tgcgaaccgg caagcaggtg
     1741 gccgaggccg gcaagcaggg ccatgcgggc gcgccgctcg tgtggagcac cgcaggctac
     1801 ggattgctgg tggattgcga aggcgcgacc ttcgaccttg ccggcggcag catcacggtc
     1861 ggtgactaca aacgtcccga taccgactac tacatcctcg ccggcacgcc cgcggaagtg
     1921 ctcggcgaag tgtcgaggat cagcggcccc gcgccgctgt ttcccaagtg ggcgatgggt
     1981 ttcaccaaca gccagtgggg catcgaccag aaggaactgc tcgacatcgt caagacctac
     2041 cgcgccaagc acatcccgat cgacaacttc accctggact tcgactggaa ggcctggggc
     2101 gagaacaact acggcgagtt ccgctggaat ccggagaagt ttcccgatgg ccccagcggc
     2161 aagctcgcga agacgctcga cggccagggc atccatctca ccggcatcat gaagccgcgc
     2221 atccacgtga ataccgtcga gggacgctac gcgacggcgc acaagctgtg gatccccggc
     2281 gagaagccgt tcgaggatta tttttcgcac aagctggtga agcggctgga cttcgacaag
     2341 ccggagacgc gtcgctggtt cggcgaggcg gcgctgaaat acgccttcga caagggcatc
     2401 gtcggctggt ggaacgatga agccgacgag atcggttccg acaccgagtt cctgaacatg
     2461 gaacgttcgc tctacgatgc gcagcgcgcg gccagcgacc agcgcgtgtg gtcgctcaac
     2521 cgcaacttct ggctgggcgc gcagcgctac gcctacggcc tctggtcggg cgatatcgac
     2581 acgggtttcg agagcatggc cgcccagcgc acgcgcatgc tgagtgcgat cgaagtcggg
     2641 gccaccgatt ggggcatgga cggcggcggc ttcaacggcc atccctcgga cgaaaacttt
     2701 gcgcgctgga tcgagttcgg cgcgttcacc ccgatcttcc gtgtccacgg caccatggac
     2761 gaaaagcgcc agccctggcg ttacggcccg attgccgaag ccgcggcaac ccgcgccatc
     2821 cggctgcgct attcgctgat cccctacatc tacgcctacc agcacgcggc gcacgccagc
     2881 ggcgtcgggc tggtgcgccc gctgacgttc gactggccgc acgatccgaa ggtgcgcaat
     2941 gatgtcgatg cctggctgtt cggccagtgg ttgctggtcg cgccggtggt cgagaaagga
     3001 cagacgtcca agtccatcta cctgccggcg ggcacctgga ccgactggtt caccgggaag
     3061 gtgcaccagg gtgctcgaac catcgactac aaggtggacg cgaaaacctg gcgtgacatc
     3121 ccgctgttca tccgcgcagg cgcgatcatt ccgacccagc cggtgatgga ttacgtcggg
     3181 cagcatccgt ccaagacggt gacgatcgag gtgttcccgg ccgaccacgc gacgcacttc
     3241 gactattacg acgacgatgg cgagacctac gcgtacgaaa agggtgtgta tttcctgcaa
     3301 cggctgacgg cagggcgcga tggctccgta gtctccttcc gcgccgatgc gccgatggga
     3361 agctaccggc ccgcgttgcg cgattacctg gttcggatac atggaaccgc tgcggcttcg
     3421 gtgcaaaccg gcggcgcgga actccgccgc tacgccgacg cgaaggcact cgcgcgcgcg
     3481 caggggcagg gctggaccac cggacacgac cagtacggcg cggtgacgat cgtcaagctc
     3541 gacgccgggg tcgtgcgcga cgtgacgttg cgcgggtccg cgcaacagtg atgccgcgct
     3601 gcgggcgccg tgcgcccgcg ccggcgcggg gagtgcctgc cttgctgcgc aaggcgccgg
     3661 tatgtcgcag ggcggacacg tccgcgggat gagatgcggg atgtctcgcg tgatccggcg
     3721 gggccgcggt gaagctggac gcgcgcgccg cgttgagcga gactcgccga tgatgtaaac
     3781 gcttccatcg gccacgccag cagggactcg ccatgaaaaa aatcgccaag cgtgttcgat
     3841 gttttgcact tggtatcgcg gcgtttgcgt tgttcgccgc gccgggactc gcagtggcgg
     3901 cagccgcgcc ggcgccgcaa accatccagt tgaaggtgga tgcccgggct tccggcacgc
     3961 ccttcccgca tttctgggag cagatgttcg gatcggggcg ggcggtactg tcgttgcgcg
     4021 acgactaccg caaggatctc gacgcggtgc accaggccac cggcttcaca tacgtgcgct
     4081 tccacggcat cttcgatcgc gacgtcggcc tcgtgcagcg cggccaggac ggcaggatca
     4141 gctacaactt ctcctacgtg gaccagatct acaacgggtt gctcgcgcac gggatgaaac
     4201 ccttcgtcga actcggtttc atgccgccgg acctgagttc cgatccgtcg aatcaccaca
     4261 gtttctggta ccaccccaac gtgatgccgc cgaaaaacta cgcggagtgg gatgcgatga
     4321 tccgcgcctt cgcgcagcac ctgatcgacc gttacggcat cgacgaagtc gcgagctggt
     4381 acttcgaagt gtggaacgag cccaacatcg acttctgggg cggtgagccc aagcagtcga
     4441 cctatttcga gctctacgac cacacggccc gcaccttgaa gtccgtgagc gatcggttgc
     4501 gggtgggcgg ccccgcgacg gcgcaggcgg cgtgggttcc agcgttcctg gcacacgtgc
     4561 acgaggcagg catacccatc gacttcgtca gcagccatgt ctacggcgac gacaccgccg
     4621 agaacgtgtt ccacaccgac gaacacatcc cgcgcaagga catggtgtgc cgcgcggtcg
     4681 ccaaggtcca cgacgaaatc ctggcctcgc cttatccgaa gctgccgctg gtcttcagcg
     4741 aatacaacgc ttcgtatgcc aacctgccca acgtcaccga cacggtgttc atgggcccgt
     4801 ggctggccaa taccatccgc gcctgcgccg gcaaggtggc gatgatgagc tactggacct
     4861 tctcggacgt gttcgaggag cagggcgtgg tgcgcacgcc gttctacggc gggttcggcc
     4921 tcatcgccga ggaccgcatc gacaagccgg cgttcaacgc cttcgcgatg ctgcacaagc
     4981 tcggcggcga gcggctgccg ctgtcgtccg acagcgcgct cgcgacccgc cgacccgatg
     5041 ggtcgctggt gcttgcgctg tggaactatg caccgccggt cggcagtggc gcgacgtata
     5101 tgccgggccc gccgcaaggg tcgccgaagc atttcgagat caccttccag cacctcgcgg
     5161 cgccggctac cgcggcggtg tggcggctgg attccatgca tggcaacgtg atcgccacct
     5221 tcgacaagat gcggcggccg gctttcccca gccgcaccca gatcgagcag ttgcgcgccg
     5281 ccggcaggct tggcgatccc cagcaggtgg gcgtgcacga cggcaggttg acattcgaca
     5341 ttccgccgca aggcctggtg gtcgtggaga taccgtcaac gaacacgcgg cacgcgaacg
     5401 ccgccgaaaa gccaggagca ggaacgtgat cccgaccgca ttgttgatgc tggcaacgac
     5461 cgccatgcag gctgcgcctg tctggcacgc gagcctcgaa tggcgcggcg cgtccgcttc
     5521 catgtctgcg caggccgatg gcagctacgc gctcacgggg ccgttcggtg aacgcaggat
     5581 tccggccccg cccgcgaagg cgcaaaccgc gagcgtgctg ttcgacggat tgttcgcgat
     5641 ggcgcaggag gaactcgccc aggactcggt gtcggtgctg catgatcccg cctacgacca
     5701 cggcggcgcg atggattgcc gctgcttcgt caccggggtg aagtggccgt acgtgtggac
     5761 ccgcgacgtg tcgtattcga tcgacctcgg cttgtggcga tacgacccgg cgcgcgcccg
     5821 cgaatcgctg ctgttcaagc tgtcggacgt gcgcgcgaag gacgtgccgc aaggttcgta
     5881 tcccatgcag gacaccggct ccggcggcag ttggccgatc agcacggacc gcgtggcgtg
     5941 gttcctcggt gcgcgccacc tgctcgacga ccgggcgttc gccgacaaga cctggcgcgc
     6001 cctcaacgac accctggctc aggatcgcgc atacgcattc gacccaggcc ttggcctgta
     6061 ccgcggcgaa acctcgttcc tggactggcg cgagcagacc tatcccgcgt ggacgaagga
     6121 caacgtggtg ttcatcgggc agtcgttcgc gttgtcgacc aacgtgctgc attacgaggc
     6181 gttgcggctc gcggcacgga tggcgaacga gcgccacgac ccgcgcggcg cggagtacac
     6241 ggcgcatgcc gacgcgttga agcaggcgat caacgcgcac ttctggcgcg acgatcgcgg
     6301 catgtacatg agctacatcg gtggtgacgg aatgccggtc gaagcctacg atctgctcgg
     6361 cctgtcgctc gcgatcacca gcggcgtcgc cgacgaggcg cgtgcgcgct cgtcgctttc
     6421 gcattatccc gtgtggccgg cggggagtcc ggtgatctgg cccgagcgcg ccgaccagcc
     6481 gatctaccac aaccgcgcga tctggccgtt cgtcagcgcc tacgccttgc gcgcggcgcg
     6541 caagatcgat gatcccgcgc tgatcgcgct cgagatccgt tcgatgatcc gcggtcccgc
     6601 actcgccggc tcgaacatgg agaactacgc catgttgagc caggcgacgc atgtcgacga
     6661 aggcaagctc agcggccccg tggtcaattc gccgcgccag ttgtggtcgg tcgcgggtta
     6721 cctcgacatg gtggcggagg gcgtgttcgg gctgaccgcc gacggcaagg tggaaccgaa
     6781 gctgccggtc tcgctggtgt cgacgctgtt cggggaccgc ggcgaaatca gcctgcaact
     6841 tccgcaccgg cagatcacgc tgcggcggcc tgacaggctc gacggcaacc tgctggtggc
     6901 cgacaaggtc gagacccatg gcgaccgcac cgtcgtcatg cttaaggctc tcgaggttcc
     6961 cggtttgaat ttgcgcaccg atgcgccgct gtatgcgccg gccacgccgg cggcaccgga
     7021 cgcgcggcgc gacggagaca cgtggaacgt gcgcagcatc gaagacggcg accacgtgtt
     7081 gtatgtcgat ggcaagcgtc atggatccgc cggcgacgca tggacgttgc gggcctcgga
     7141 cctgcggtat tgcctgcgcc tcacccgcct gggcgagcac ggtatcgaat cattgccgag
     7201 cgaggcgacc tgcatgggcg acgacgcccg tgtcaccgga tcctggccgc gcgcctggac
     7261 cgcgccccgt ggcggccgtt accgcgtgtg gctggactat acgaacgatc acggcccgat
     7321 caacaccggc atcaccgtgg gggtgaagcg gctcgcgatc gactgcacgg gtagtcctgc
     7381 gcagacggtg acgatcgtga tgccgcacag cgttgccgaa caacgttcca ccgccgccac
     7441 cttcgagacc aaggcgggcg ctcgctgcac cttcgcgctg gaacaaggct tcaacatgag
     7501 cgacctcgcg catttcgtgc actacaccgg cggagggggc ggcgcgagcg gtcccctcaa
     7561 cgaggcgaac gtcaacgccc tgcacgttgc gccgctgccc tcacagtgat gccctgtggc
     7621 ttgcacgggg tcttcgtctg gatttcctgc tccgccggga gaaggtggcg cgcagcgccg
     7681 gatgagggta cgggtacgca aatggtaggt tcctggatta ctcaccctgt catgcgggcg
     7741 cgtaccctcg cccgcctctc cggtgcgcta cgcgcttgcg ctgcgcgcgc ctctcccggt
     7801 gggagagggg accaagcaga aaccgatcgg gaaactccat gacccggaaa cccgcgctgt
     7861 cgttctggca gatctggaac atgtgtttcg ggttcctcgg gatccagttc gggttcgcgc
     7921 tgcagaacgc caacgtcagc cggatcttcc agacccttgg cgcgggcgtc gatgcgatcc
     7981 cggcgttatg gatcgcggcg ccgttgacag gactgatcgt ccagccgctg atcggccatt
     8041 ggtccgaccg cacgtggggg cggctggggc ggcgacggcc gtacttcctg gtcggcgcgg
     8101 tgctcgcgac gctggcgctg ctggtgatgc cgaacgcgcc gttgctgtgg gtcgcggccg
     8161 ggctgttatg gatcatggat gcctctttca acgtcgcgat ggagccgttc cgcgcgttcg
     8221 tgggcgacca gttgccgacg cagcaacgcg cccgcggcta cgcgatgcag agtttcttca
     8281 ttggcgtcgg cgccgtggtg gcatcgttgt tgccgtggct gctggcgcgc ttcggggtgc
     8341 ccaacaccgc gccggaaggt gcgattcccg acaccgtgaa gtacgccttc tatgccggcg
     8401 gcgcggtgct gctggggtcg gtgttgtgga cggtattcac cacccgagaa tatccgcccg
     8461 acgcgttgct ggcgttcgac gacagcgccg aagcagcgcc gacgagcgac gtgtcccgcg
     8521 cctggcggat cggcgccgcg ctcggtacgc tcggtgtgct gttgctggcg ctgatcctgc
     8581 gcttctcgct ggcgcgcgac ctttacctgc tggccggcat gctgatcgcc gcaggggtgc
     8641 tgttcgcgtg gctgtcccgc acccgtagcg ccggaaccgt gcgccaggtg atgggcgacc
     8701 tgtaccggat gcccgaagcg atgcggcgcc tcgcgtgggt gcagttcttt tcgtggttcg
     8761 cactgtttgc gatgtggatc tacaccacgc cggcggtgac cgcggtgcaa ttcggcgcga
     8821 ccgacccgca ctcggcggcg tacaacaccg gcgccaactg ggtcggcgtg ctgttcgcgg
     8881 cctacaacgg cttcgcggcg ctggccgcga tcctgattcc ctggatggcg cgggcctggg
     8941 gcctgcgcgt gagccatctc gtcaatgtga ctctgggcgg ggtggggctg atctcgttcg
     9001 cgctgatccg ggatccgcac tggctgctgc tgtcgatgct gggcgtcggc ttcgcctggg
     9061 catcgatcct gtcgctgccc tacgcactgc tctcggacaa cctgcccgcg gccaagatgg
     9121 gcgtctacat gggcatcttc aacttcttca tcgtgatccc gcaactgctg gctgcaagcg
     9181 tgctgggcgt gctgctgaag tggttcttcc acgaccagcc gctgtacgca ctggtgctcg
     9241 ggggcgccag cctgatcgtc gccgggttgt gcacgctgcg ggtgcgcgag ccggacgccc
     9301 cggtatccgc gggggccggg gcggcggctt cgcccgggta acgttcgcgc gtcggcgaaa
     9361 cgtgccggta cttgacggtg atcaagacgt agcgctgtgc cagcgggaca atatccacgg
     9421 ctgcacccgg cgtggcgaaa caggcatgga attccacatc aagaccctcg gcgcacggcc
     9481 ggacctgggc gcgatcaacg atgcgttgat tgtcgccgac ccgatggcga tggccgacct
     9541 cgacccggca ggcgggaccc tgcgggtcgc tgcggaattg agctcgcccg aactgctttc
     9601 gttgttgacg cggagcgggt atccggtcga ttggcggcaa ctcgagcagg tgccatccgt
     9661 gtgttgcggc gcctgcggcg ggtaaagcga agcgcccccg ggtccggccg cggggtgccc
     9721 gcccaccgac gttgcgggca ggccacggcc gcttcatgct ggagttacgc aggcgccggc
     9781 taaatcggta cctgcgagca gcgagaaccg gtcatggatg acgacgtacg cggacccacg
     9841 cgcgcccgcc tcaccgaaca cacgcgcgcg gagatccgcg ccggcctcga aggccgccgc
     9901 cgcggcctcg ccttgctgct gccgttcgcc ggtcccgccg tggtggtgtc gaccgcctac
     9961 ctcgaccccg gcaacttcgc ggtcaacatc caggccggcg cgcgctacgg tttctcgctg
    10021 gtctgggtgg tgctgctcgc cagcctgatc gcgatgctgt tccaggcgct ggcggccaag
    10081 ctcgggatcg tcaccggccg caacctggcg caactgtgcc gcgaacgcat gccgcgtccg
    10141 ctggcgatgt cgatgtgggc ggtgagcgag gccgcggcga tggccaccga cctcgccgag
    10201 ttcgtgggtg gcgcgatcgg ggtgtcgttg ctcctgcaga ttccgatgat ggcggggatg
    10261 atcatcactg cgatcgcgac ctgcgccttg ctgctgctcg agcgctcggg ctttcggcca
    10321 ctcgagcttg ccatcggcag tttcgtggtg gtgatcggcc tgtgctacct ggccgaactc
    10381 gtgatccagc cggtggcgtg gccgggtctg ctcgcgagcc agtggccgcc acggttcgcg
    10441 gattccggcg cggtcctgct ggcctgcggc atcgtgggtt ccaccgtgat gccgcacgca
    10501 ctgttcctgc actcgggcct ggtgcagcgg cgcgcgacgc cgcggacgcc gcaacagcgg
    10561 agcacgttgc tgcgctttgc gcgcatcgag gcggtggtcg cgttgggcat tgccgccgtc
    10621 gtcaacatgg cgatggtcgt catggccgcc gacgcctttt acccggcccg cgcgcaggat
    10681 gcggtgctgc aatccgctta ccacacgctc ggtccgttgc tggggccggc cgccgcgggc
    10741 gtgttcctgg tcgcattgat tgcatcgggg atatccagct cggtggtggg cacgctcgcg
    10801 ggacagatgg tgatgcaggg cttcgcaggc ttcccgctgt cggtatggct gcggcgcgcc
    10861 gtcacgatgg ttccgagctt tgtcgtggtc gcgctgggga tcgatgcgac ccgggcactc
    10921 gtgctcagcc aggtggtgtt gagcctggcg ctgcccgtgc cggtggcggc gctgttgtgg
    10981 ttcaccggcc gccgcgacct gatgggccgg taccgcaacg gattgccgac ccgggtcgtc
    11041 gccgccgtcg cggcaggcgc cgtcgtagcg ttgaacatcg tgctgctggt ggagtcggca
    11101 gcgagcaacc catgacggca cggtcgcgag cgtccgcatg ggcaggagcc cggagtccgc
    11161 tcgatttttt tcgccgggca ggcaaaatcg ccgcacttcg caaaaaagat tcctctgcgt
    11221 ccgcaacaat ttccggccgc ggcacgctcg agcgcccgcg cagcttgtcg cgcgagcgtg
    11281 gcacgcatga tgcaccccga aggccggagc attcagcccc cgcggacgtc cgcccatcgt
    11341 gcagctgcgc ctgccgccgt ggcggtcgct gtcgtgcgca atggaaaagg agttcgaatc
    11401 atgatccagc agcaaaccgt ttccctggac gatgcgcgcc gcgtcatcgc cgcgggtgag
    11461 gacaaggcgc gcgagattgg ccagcccatg aacatcgcgg tggtcgacga tggcggcaac
    11521 ctcgtggcat tcgcgcgcat ggatggcgcc tggaaaggta gcgtcgacat cgccgtaaac
    11581 aaggccttca cgtctgcgat gtttacgatt cccacaaagg cgctcaccaa aaaggctcaa
    11641 ccgggtgaac cgttctacgg aatccacgtc tccaatcacg gcagggtaat gattttcccg
    11701 ggcggcgttc cgttgaaagc gggcgatcaa gtgatcggcg ccgtgggcgt gagtggcggt
    11761 gacaattcgc aggatcaggg cgtggcagaa gccgccgcgg atgccctttg aaagacgagc
    11821 gaaaggatcg ttttggccgg agcttcatca cccgataaca aagcggctct gcttgacacc
    11881 tgaaattcac aagcgtacgt ttttttgaga ggcgtatgaa tcggcaagga ggccgattat
    11941 gcgtaaatcg atttttctgc tggtctttgt gttgggcctt ccgctcgcgg cgggggcagc
    12001 ccagtctccc cagtcgtcct cgcaggaccg aagctggctg atcgccgcgc accaggacaa
    12061 tctcgccgag atcgagtcgg gcgagctggc ggcggcgaag ggccacgatg cggacgtgcg
    12121 cgcggcggcg cggacgctga cccgcgatca cggcgatctg gacgccaagc tgcggccggt
    12181 cgccaagcag ctcggcgcaa aactgccgac gcagccgaat gccgagcagc gcgacgagat
    12241 gcgcaagttc aagagcgcat cgggcgtcga cttcgaccgc acctggaccc acgacgaggc
    12301 cgatgcccac gtcaaagcca tcgaattgac cgtgcgcgag atccagcatg gttcgctgcc
    12361 acaggtgaag cagctcgcgc agtcggcact gcccgttctg aagaagcatt tgcacatgct
    12421 gcagcagacc cagtcggcgc gcagcggcaa tcaatgagga atccacgcgc gcccggtcgt
    12481 gacgccggtc gtgcacgcaa ggaggcatcg atgaaaatgc tcgctctcgc tacggccgtt
    12541 tcggcagccc tgctcgcgac atcgatggcg cgtgcggcgg tgcatccggc caagccagcc
    12601 tctgccggca acgcagccgc gcaatcggcg acgggcgaga gcgtcacgca gttggccagc
    12661 aatccggccg actggccgat gccgggcaag aactacgctg gcacgcgctt cagtggattg
    12721 aaacagatcg acgcgaacaa cgtcaggaac ctgtcggtgg cgtggacgtt ctcgaccggg
    12781 gtgctgcgcg gccacgaggc ggcgccgatc gtggtcggcg acacgatgta cgtcgtcacc
    12841 ccgtggccgg acattctgta cgcgctcgac ctctcgaagg acggcgccat caagtgggtg
    12901 ttcaagccat acccctcgcg cgcgtcccag ggtgtcgcct gttgcgacgt ggtgaaccgt
    12961 ggcgttgcct actccgacgg caagatcttc atgaacaccc tggacgatca cacgatcgcc
    13021 gtcgacgcga agaccggcaa gcaggtctgg aacaccaaga tcggggacat caaccatggc
    13081 gaaaccatca cgatggcgcc gctggtggtg aagaaccacg tgctggtggg tgtcagtggc
    13141 ggcgaactgg gggtgcgcgg ctggctgaaa gcgctggaca ccaataccgg ccaggtgcaa
    13201 tggacggcct attcgaccgg cccggacaag gacgtattga tcgggaagga tttcaatccg
    13261 ccatatccgg aagacaaggg ccccaacctc ggcgagacga gctggccgaa caaggccgcg
    13321 tggatgatcg gcggcggacc ggtttggggc tggatcacct acgacccgaa acaggatctc
    13381 atttattacg gtaccggaaa tccggggcca tggaatgcct accagcgccc cggcctcaac
    13441 ttctggacct gcacgctatt cgcgcgcgat cccgataccg ggatggcgaa gtgggcggtg
    13501 cagtggaatc cgcacgacac ccacgattac gacggcgtca atgagaacgt gctgttcaac
    13561 gcgacgatag acggcaagga gcgcgagctg ctggcgcatc ccgaccgcaa cggcttcatg
    13621 tatgtgcagg atcgcaccac cggcaagatc atttccgcgc agcccttcat gcctggtacc
    13681 aacacgatcg agcgtatcga tctggtgact ggggcgccgg tggtgaatcc cgagaaggtg
    13741 ccgcagctcg acaagacagt gcacgacatt tgccctgcat cgaccggcgg caaggactgg
    13801 cagccgagcg cgtattcgcc gatgacgggg ctcctgtaca tcccgcacaa ccacctgtgc
    13861 caggacgagg aggtcagcaa ggtcagttac atcgaaggca cgcctttcgt gggggccaag
    13921 gtggcgatga aggcggcgcc gggcggttgg cgcggcgcat tcaccgccta cgacccggtg
    13981 cacgcgaagg tggtgtggtc tgatcacgag aattttcccg tgtggagcgg tgcgctggtc
    14041 acagcgggcg gcgtcgcctt ttacggcacg atggatcgct ggttcaaggc ggtggacgca
    14101 aaaaccggca agctgctctg gaaattccgc gtcggttccg gcatcagcgg ccaaccgatc
    14161 gcgttcaagg ggccggacgg ccgcgaatac gtcgcggtgc tggccggcgt cggcggctgg
    14221 tccggcgcgg tggtggcggg cgacctggat ccgcgcaatc cctatggtgc gctcggcttc
    14281 gtcggcgcga cccaggacct gccggactac accaacaagg gtggaaccct gtacgtgttc
    14341 gcactgccga agccgggagg ctcgccatga aggcgcggat gctgctcctc ctcgccctgg
    14401 ccgccgctcc cgccttcgcg ttcgcgcaaa cccacacgca gtatccggga gcggcgccgg
    14461 cgacggccag cccgtcgctg cgggtgtgcg ccgatcccaa caacctgccg ttctcgaacg
    14521 aagccgagga gggcttcgaa aacaagctgg cgagaatgct ggcgcattcg ttggggaagc
    14581 aggctcaatt cgtctggcac gagcagggca aggagtttct gcgggatacg ttgaatgccg
    14641 gcatctgcga tgtcgtcatc ggcctgccga ccgacgacca cgcgtacggg gtgctcacca
    14701 ccgaccccta ttaccgctcc gcctacgtgc tggtctatcg ggccgatgcg ccgtaccagc
    14761 tgaaatcgct ggacgatccg cggctgcgga cgctggagat cggcgtgcac gcgaccggcg
    14821 acgactgggt ggacttgccc ggcggtgcga cgctcgcgca tcgcgggatc gtgaagaacg
    14881 tgcacgtgta cacgctgggc gtcgacgaca gccacgcgaa tccttccgcg gacctggtcg
    14941 atgcggtcgc caagggggat atcgatgtgg ccatcgcctg gggccccctg gccggctact
    15001 tcgcttcgcg tgaaccggcg aaattgaagc tggttccgct gaccgatacg cacacgctcc
    15061 tgccgttcca gttctcgatc tcgatggcgg tccggcacgg caacggagcg ctgcgggaca
    15121 agttgaacgc gttcctcgcg acacaccgcg cagccgtcgg gcaattgctg gcgagatacg
    15181 gcgtaccgca attgaacatt cccgcagccg gagcacctcg actgcaggcc ggtttcgcgc
    15241 gcagcggagc atcggagtga ggcgggcagg gccaaggagg cggtcatgaa aacggtatgg
    15301 gtggccctcg tgctggcgtt cgcgctcgcg gcgaccgcgt tcggcttcgc gcaggtggcg
    15361 agcccgcggg tccaggcttc gccggcgcaa cagctcgaga acactccggt gtcggacctc
    15421 cacatcggcg tgccggcaca aatgaagaac ccctatgcgg gtgaccgcga tgcatggatg
    15481 cagggcaaga agctgttcaa ggccatgaat tgcgcgggct gccatgcgcc cggcggcggc
    15541 ggcggcatgg gtccgccgtt gtccgacagc cattggatct atggcggctt gccgggccag
    15601 atctacctgt cgatcgagca cggccgcccg aacggcatgc cgcaatgggg caccgtgttg
    15661 ccgtcgaagg cgatctggga actggtgacc tacgtcgaaa ccctcccgca gccgaatccg
    15721 gaattcgaac cccagaccaa gctcggggtg aaacccatgc ccggcaacac gccgcacccg
    15781 gcgacccagc gggtgccgcc acgtggcccg aaacaccaga agccgacgcc gaagccggca
    15841 tacccggcgc ccgcctcgac acccgccaag gcaccgccgg cggcccctgc ctcgaccacg
    15901 ggcaagcatt gatgcgccgg ctccccgcca tcctggtgat gcttgccccg gcgctcgcgc
    15961 atgccggccc catgaccttc atggtttcgc atggcgcggc ggccaatccc atcaccaaac
    16021 tgggttgggg cctgctcgcg ctctccattt tcgtgggcgc cttcattgca ctcgcggtgc
    16081 tgatcggcat ctggtggaag cgcggcgcgg gcgaatacga cgaacgcggc tggccggtac
    16141 tttcgcacgg gcgcaacggc cttccgtggc tgtggatcgg ggtcccggtc tccatcgtgc
    16201 tgttgctgct ctcggcgatc tggaccttct acgtgctcgg cgtggccacg gcggcgccgg
    16261 gccaccctaa gatcacggtg gaagtgatcg gccaccagtg gtggtgggaa gttcgctaca
    16321 gcgaattcgg ttaccccagg ctcagcttcg acacggccaa cgagatccat atcccggtcg
    16381 gggtgccggt cgaggtgcat ctgaccagcg cggacgtgat ccactccttc tgggttccgc
    16441 aactcgccgg caagaccgac acgattccgg gccagatcaa ccacgcatgg ctcgaagccg
    16501 acaagccggg cgtgtatcgc gggcaatgca cggagttctg tggattgcag cacgcgcaca
    16561 tggcgttgta cgtggtggcg caaccggcgc gccagttcaa ggcgtggtgg aagcaccagt
    16621 tgcagccggc tgccgaccag gtcacgggat cggcgggcga gggcgaagac gtgtttcgaa
    16681 cgcgctgtgc gatctgccac accgcgcgcg gaatgccggg aagccagggc aagctcggcc
    16741 ccgacctcac ccacctgatg ggccgccgca cgattgccgc cggcacgctt cgcaataccc
    16801 ccggcaacct cgccgcgtgg gtgagcgacg cgcccgcgca aaagccgggc gtggacatgc
    16861 ccgccgagga tttgaccggt ccgcagttgc aggcgctgct ggcctatctg aagacactga
    16921 aatgaacgtc gagtacgcaa tgggtcaggt cgcgagcacg ctcgctcctg caatagccgg
    16981 ttcgtaggag cctgcgcgca ggcgaacgac aaggaagcgc aatggccgcc aatcccacct
    17041 tccaaacgcc cgagatcgtc gtcgccgacg aggaattgcg ccacaagctg gagcagatgt
    17101 ggtccgatcc gccgggcatc tacggttggt ttgccaccac cgaccacaag aagatcggca
    17161 agcgctatct cttcaccgcg ttcgtgttcc tgatcgtggg cgggctggaa gcgctcggga
    17221 tgcgcctgca gctcgcgcag ccgaacgagc acctgctgtc gccggaactg tacaaccagt
    17281 tcttcagcat gcacgggttc acgatgatct tcctgtacgc gctgccgatc ctgtcgggct
    17341 tcagcaatta cctgtggccg ctgatcctcg gcacgcgcga catggcgttc ccgcgcctga
    17401 atgcgttcac gtactgggtg ttcctgctgt ccgggatctt cctctacatc gggttctgga
    17461 acggcaaggg caccgacgtc ggctggttca attacgtgcc gctggccgag cgcgcctaca
    17521 accccgactg gaacgtggac atctattcgc tggggatgat cttcctcggg atctcgacca
    17581 cggccggcgc ggtgaacttc atcgccacca tgttccgcac gcgtgcgccc ggcatgtcga
    17641 tcagccgcgt gccgatcatg atctggggca cgctggtgtc gaacattggc atcctgttcg
    17701 cggtgccggc ggtcagcctg gacttcctga tgctgtggct ggatcggcgc ttcggcaccc
    17761 agttcttcaa ttcatccgcg ggcggccgcc cactgctgtg gcagcacctg ttctggatct
    17821 tcgggcatcc ctgggtttat gcgatcgtgc tgcccgcgat gagcatcgtc tcccagggcc
    17881 tgccggtgta ttgccggcgg ccgctcgtcg cgcattcggc ggtggtgctg gccacgatcc
    17941 tgacgatggt gctgggcttc ggcgtgtggg tgcaccacat gttttcaacg ggcttgccgg
    18001 tgatcgcgct gggtttcttt gccgccgcga gtttcgtgat cgtgattccg tccgcggtgt
    18061 cggtgttcgc gtgggtcgcg acgatatgga ccgggcggcc ggtgttcacc accgcgttca
    18121 agttcttcgc ggccgagatc gtgctgttcg tgatcggcgg cgtatccggt ttcatgaccg
    18181 ccgcggtgcc gctggactgg cagctcaccg acacctactt cgtggtggcg cacatccatt
    18241 acgtcctgat tggcatcaac gtgttcccgg tgatcggcgg gatctaccac tggttcccca
    18301 agatgaccgg ccggatgatg aacgagcggc tcggctggtg gaatttctgg ttgatgttca
    18361 tcggcttcaa cctcggcttc ttcccgatgc acatctccgg gctcgaggga atgccgcggc
    18421 gcatctacac ctatcccgcg aacctggggt ggggcgacct gaacctcgtc accaccatcg
    18481 gcgcgttcgt attcgcgctg ggcgtgctgc tgttcgtgat caacgtggtc cgcagccgct
    18541 ttgcgggtgc acctgcacct gccaacccgt gggacgcatc aaccctcgaa tgggcgacca
    18601 cgtcgccgcc acccagccac aacttcaccg tgatcccgca ggtggcgagc ctggacccgc
    18661 tgtgggagga gcgcctcgaa ggcggccgcc agagcctgat caccgaaggg ccggttctcg
    18721 acgacgagca cgagatcccg ggcaccacgg tgctcgacgc cgagccggac gtgatcatga
    18781 agatgccgca tgcgacgctc gcgcccatat cgctggcgct gctgatcgcg ggtaccttcg
    18841 tcgggcttct gatgtcgatg tggtgggtgg cggccggttg cctgctggca atgattccgg
    18901 cgctcctgtg gtggttctgg ccgagcccgg aaatgggttt cgccgagagg gtgcggccgt
    18961 gaccgcctac gacgaacgca cgctgccgcg gccgctgccg atcgggccgg cgggttggcg
    19021 gacgccggga tggtggggcg tactcgcgct gatcggcacc gaggccgcag tgttcgcgta
    19081 cctcctgttc agctacttct acctgcaatc gcagacgacc gaagcgtggc ccccgagcgg
    19141 gctgccgcca ctgcgctggt cggcgccgag caccgccgtg atgctgatca gcgtcgccac
    19201 gacttggtgg gccgatcatg gcatgcggct cggcagcatt ggccggctgt attccgggct
    19261 ggccgcgacg tcgctgctcg gcatctgcta catcatcctt cagtgtttcg attggcgcgg
    19321 cgaaccgttc ctcgccaaca gcggcgcata tggctcgttg ttctacgcga tcaccgcctt
    19381 ccacgtactg cacgcggcgg cgggggtcct gatcctgttg gcggtgctcg gctggagcgt
    19441 gatgggttac ctcaccccgc ggcgaaactc cgcggtcacc gtggtggcca tgtactggta
    19501 tttcgtggtg gtggcctgga tcgccgtgtt cgtttcgctt tacctcctgc cgtggatttc
    19561 gacgcactga ggcatcccat gcgagccgtc acccgcccca atccccacgt gcccgcccga
    19621 attcccgtcg gctgggcgct gacgttcgcg gtattcgggg cacccctcgc gtggatgctg
    19681 caactttgct tcggttacgc gctcgcggcg catgcctgtt ttccgatcga cgtgcccctg
    19741 gtgaccccgg tatggccgag cctctggtgg gtgctggtcg gcatcgacct ggtcgcgctt
    19801 gccattgccg gcaccgggtt gttcacggcg tggagttgcc acctggcgtg gcgcggggtc
    19861 gatccccgcg atgtcggcga gcgccgcaac cgtttcatcg cgcagtggtc ggtgctgacc
    19921 agcgcgctgt tctccattgc cctcatattc acgatcgtga tggtcttcat cgagccggtt
    19981 tgcaattatt gagtggaatc atgaaaagcg tttttgacgc ggataaaagc ggatgaaagc
    20041 ggatagagca aagagaacag atctgatgat ttgctcttat cagatttgat cgacgtcaaa
    20101 agacgtttgc ttttatcaga tttgatccgc gtcaaacgta tttgcttcga aggtgacagt
    20161 ggaatgtcgc acctctggac agaatggagt tgggaaccct ggctgctggt ttgcctggcc
    20221 gcggccgtgg tgccctacgt cgcgggcatg ctgcggatgg gcgcccggcg tacgcagatc
    20281 ctcggccgct ggcgcgcacc ttcgtttttc ggcgggctgt tgctgctgtt cctcgcgttg
    20341 atgtcgccgc tggatgaggt cgccgacagc ctgttttcgg cgcacatgct gcagcacatg
    20401 ctgctgttgc tcgtgattcc gccgctgctg gtgtacggac ggccggtgat cacctggctg
    20461 tgggcattcg ataccgattc ccggcgcgcc gtggtgcgtg gctggaaatg gtcgaagctg
    20521 gaagccatgt tcgcattcct gatgcggccc ctgtgcgtgt ggacgctgct gaccgcagcg
    20581 ctttgctact ggcacttgcc gggaccgtac gacgccgcgg tgcgccacga gtggctccac
    20641 gacctcgagc acctgagctt cctcgcgttc gcattggcgt tctggacgat cgtgatcgag
    20701 ccgtacggcc gccgtcgcgc attgggctac ggcgcgacag cggtgttcgt ggtgtcatcc
    20761 ggcttcgtga tgagcctgat tggcgcgatc ctcaccttcg caacgacacc gctgtacgcg
    20821 gtacacctgc agaccaccca ggcatatgga ttgacgcccc tcgaggacca gcagctcgcg
    20881 ggcatcatca tgtggattcc ctcgaacctg gtccacgtcg gcgcgctcag cgcagtcttc
    20941 ttcgcatggt tccgtgccga cgagcgtcgc gcgcgcgagc gcgtcgcacc ggcgcgcgcg
    21001 acctcgcgca ccgccctcca tacattgctg gtattgccgc tggtggcggg aatgctcggc
    21061 ggctgtgccc atcgcccttc gcccgaccag gtcgaagcgc ggcgcggcgc gcacctgatc
    21121 gagcattatg gttgcgggtc gtgccacatg atccccggca tcaatggcgc cgacggcctg
    21181 gtcgggccgc cactgatcca ctggagccgc cggacctaca tcgcgggcgt gcttcccaat
    21241 gacccggaca acctcgcctt ctgggtccaa cacccgcaaa aggtaatccc cggcgtggac
    21301 atgccggaca tgggcctgaa gcagcgcgac gcacaagcca tcgcggccta cctcgacacc
    21361 atccaatgag tcgcggcggt gatcgtcgca tcacgaggcg tttgacgcgg atgacaacag
    21421 atgactccga cagagcaaga gcatttgctt tttagcggca cttgtccgcg ttggtcggtg
    21481 tcaaaaaata cacaaccacg gaaggtacaa agccatgcga gcagtcacgt ttcacggcaa
    21541 ggaagatttt cgggtcgaca acgttccgga tccgaggatc gagcagccca ccgacgccat
    21601 catccggatt acctcgaccg cgatctgcgg atcggacctg cacctgtaca gcgtgctgac
    21661 gatgtttttg gaagaaggcg acatcatcgg ccacgaaccg atgggcatcg tcgaggaagt
    21721 cgg
//
LOCUS       DASSNH010000064        17985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_70725, whole genome shotgun sequence.
ACCESSION   DASSNH010000064 DASSNH010000000
VERSION     DASSNH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70725"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFK00_02285"
     CDS             <1..582
                     /locus_tag="VFK00_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7591816.1"
                     /translation="RDAGGDAEVAAFDVTDAEAARAAIDELLESGPIHALVHNAGIHD
                     DAPLAGMNADQWRRVIDVSLNGFFHVTQPLLLPMARARFGRIVAVSSIAATMGNRGQV
                     NYAAAKAALHGAAKSLAREMASRNVTVNVVAPGVIEGRMTQALFTEDQIKAMIPAART
                     GTPAEVAGVVAFLCSDAASYVNGQVIGVNGGMA"
     gene            complement(658..2238)
                     /locus_tag="VFK00_02290"
     CDS             complement(658..2238)
                     /locus_tag="VFK00_02290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7591817.1"
                     /translation="MAAATTSDVLIVGGGLAGLTLALQLRERCPQLSIRVFERRNGPA
                     PAAAFKVGESTVEIGAHYFADVLGLREHLDGAQVRKFGFRFFFSDGCAHLDQVPELGI
                     SRVFPTPSWQVERGTLENFLATRARERGIAIEQGASVRSIEPGGDGAMHAVKAGDAEH
                     RARWLVDASGRAGLLRRKLDLSEDNGHRIGAAWFRVEARLKLDDWCEDPAWRGLCEPQ
                     ERWRSTNHLCGAGYWVWLIPLASGAHSVGIVADTTRHPVESFDTFERALAWLRVHQPA
                     LAGRLEDQRDALLDFRFLREVSYGCRQVFSADRWAMTGEAGMFLDPFYSPGTDFIAIA
                     NTYITGLVARDFAGEPIAKPVRRCQQMFFSFYRNMLNLYLGQYELFGDARVMTAKVLW
                     DYAYYWGVLCPLVIGGRLDDFALFGDLATELADAESLNLRMQEFFRTWHRANPAHPAP
                     PWMDQQDLAWFAQLNARLGEDLDDHMIRARLRANIDLLHNLAGTLAARARSEMPTMDL
                     GGLPVVPREPLTLFRAAA"
     gene            complement(2243..3061)
                     /locus_tag="VFK00_02295"
     CDS             complement(2243..3061)
                     /locus_tag="VFK00_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7591818.1"
                     /translation="MSDATDRPFIVVPCLNEEQAIGELLAGVLAHCADVIVIDDGSTD
                     RTADIAAALPVTLIRHDTRRGKGEALRAGFRKALELGAGGVLTMDGDGQHDPADIPRI
                     LAAARRLPGALVIGARMLGRECQPNARRRANAVADWGISWGCGRPVADTQSGQRWYPR
                     AAVELVDLPAQDFVFEAAILITASRDLDMPVVSIPIACRYDGSARHSHFRPVRDVVRI
                     TWYTIGQIVHFGSVVQSYRASHGSAPEVYDPDHRLEAETSAPLRRVGSPPPARS"
     gene            complement(3058..3858)
                     /locus_tag="VFK00_02300"
     CDS             complement(3058..3858)
                     /locus_tag="VFK00_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HET7591819.1"
                     /translation="MSKARTLAVTGIGAWIRGAPDWPSLRSILRGEHDLDADAPAKPA
                     ADPLPAAERRRAPDGVRLAVEVAQQACAMAQCDPSALPCVFASTHGELAITDYVCETL
                     ASAPQELSPTKFHNSVLNAPAGYWTIATGCTAPSGAVTAHHYSFAAGLLEAAAFACTE
                     QAPVLFACCDVASTGPLAEMTRTTLAFGMALVLTPVVPGGARLDWSLRDAQSGPLPPV
                     ELALRAVAGDNPANAHALALLAALAGRESTRLALPLSPGLFLDMEITP"
     gene            complement(3855..5051)
                     /locus_tag="VFK00_02305"
     CDS             complement(3855..5051)
                     /locus_tag="VFK00_02305"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HET7591820.1"
                     /translation="MPPHIEPLAITVRTATSALGRGTAAHLGALAEGRRGLRRNDFSC
                     APLDCWIGRVEGVEEAALPARHTHWDCRNNRLAWLGLQHDGMLDAIGAAVARHGPKRV
                     ALVLGTSTASIEATEVAYRRLEDGRFPSDLRRPEIHTPHSLAAFVADATGIAGPTLTI
                     STACSSSAKVFASAERLIRLGLADAALVGGVDSLCDSVLFGFHSLELVSRQPCRPFDR
                     QRDGISIGEAAAFALLERVEDAPGAPRLIGYGESSDAWHMSTPHPEARGALAALDDAL
                     ARAGITAEQVDYVNLHGTATVKNDEVEAKLLPRFAPRTRASSTKGFTGHTLGAAGALE
                     AVIALLAIEHSVVPPDLGCTDALPEAEPWLALDPETRDIDIALSNSFGFGGNNACLVF
                     ARGPAA"
     gene            5045..5581
                     /locus_tag="VFK00_02310"
     CDS             5045..5581
                     /locus_tag="VFK00_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591821.1"
                     /translation="MASVALRLSGWASISSARPLPQARCRTLRGAAIGRVLHMEGIMV
                     CFENPKRWLPVLLAPALLLLMAFRQVPLVDPDPIAVPHNVTQAQVEQVIGRALTARNW
                     RIVKHTPGEIDAVYDPRDFSVTIAVHYDAQRIQINYVTSDHLRYEVKNGVRYIHTNYA
                     SWIKNLVTNISNGLMMAS"
     gene            5625..6527
                     /gene="hemF"
                     /locus_tag="VFK00_02315"
     CDS             5625..6527
                     /gene="hemF"
                     /locus_tag="VFK00_02315"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HET7591822.1"
                     /translation="MQAVLETVDGFLRDLQDRICSALERVDGGRRFVEDAWTRTSGGG
                     GRTRVLKDGTVFEQAGVNYSRVTGDTLPASATAHRPELAGRSWTATGVSLVVHPKNPY
                     VPTAHANVRYFEADKEGEAPVWWFGGGFDLTPFYPFDEDCVHWHTVAHDICAPFGDEV
                     YPRYKTWCDEYFYLKHRGETRGIGGLFFDDLNDWEFERCFGFMQAVGNGFLDAYLPVV
                     ERRKDVPHGEREREFQLYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPRVRYE
                     YGYAPEPGSAEARLAEYLKPREWV"
     gene            complement(6906..7286)
                     /locus_tag="VFK00_02320"
     CDS             complement(6906..7286)
                     /locus_tag="VFK00_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7591823.1"
                     /translation="MHHSRLCSMQIDCNVADIDAAARFWAAALGRPVDMDHPGSRGNY
                     RQLAALPDEPMVQLQRVDHASRVHLDIETDDIGAEVARLEKLGAAVLKKLERWTVMQA
                     PSGQRFCVVRVQRPGFAGNANRWE"
     gene            complement(7333..9030)
                     /locus_tag="VFK00_02325"
     CDS             complement(7333..9030)
                     /locus_tag="VFK00_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET7591824.1"
                     /translation="MRRSILTVLAAVACIAAACGSPAGNGNGAPAPAEKGNTATAPTR
                     ADGTAASASQPLSAAITAQGFRAYDQAISSDAMDGRKPGTPGGERATRWIVEQFKQLG
                     LEPGNHGSWFQKVPTVSTTLESPGKVKLDVAEGGGTQAFDFGKDMVAMTLQAKPHVAI
                     KDSGIVFVGYGVNAPESDWNDYKGLDVKGKTVVLLVNDPGFGDHDPDLFKGKAMTYYG
                     RWTYKYEEAARQGAAMCLIVHTSNDAAGYPWSVVMNSWGGPQLALPPSEDPAPRLPVA
                     GWITRDAAQRLFAKAGLDFDKLAAEADHRGFKPVPLKATASITLDSKIDHGSANNVLG
                     MIKGTARPDEVVVYSAHWDHLGEDDSLKGDKIYNGAVDNGTGISALLEIAKAFKAQDP
                     APQRSVLFAAVTLEESGLLGSLYYTRHPVFPMDKTVADINMDAMDVIGPTKNLQIIGS
                     GQSQLEDMLKAVLKKQGRYAISDQTPQNGFYYRSDHFSFAKAGVPALMTQTGNDKVDG
                     GTGAGTAALADYTAHRYHTPLDNFDPAWDFSGLAADTQALYEVGRELSLSDQWPEWY"
     gene            9508..11217
                     /locus_tag="VFK00_02330"
     CDS             9508..11217
                     /locus_tag="VFK00_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HET7591825.1"
                     /translation="MKTVANLSGQGLTGTDTSTADLRTGYSIDHYTMGPASGANGFDG
                     EHVWEQGPDGAVNIEQGGDALPRAIDSAYQTANLWWRPDFGGAKVEPAGDKPCGTSTC
                     AVLEVTPKGGLPFQAWFDTGSHLLVRTWQRIGGQTTTIDFSDYRGVEGVRVPHKIVID
                     NGHGKQYLLTATVSAVAFLPAQPVATYAPPKSEMKNISIAGGAAETTFPFRLINNHIY
                     THAWVNGQGPLLFIVDTGGHNILVPSTARALGIKIQGAMAATGAGNKAMDYGLAQIAS
                     LKVGDATFSDQVVGVLDFEPNGVEGVDIKGMVGFTAFKRFVTRIDYGKHEMTLIEPDH
                     FDPQDAGTPIPFVFNGDIPEVEGTFEGIPGKFDIDTGSRSALTLTGPFAQENNLRSRH
                     PKGVTAVDGWGVGGAARGYVTRGKLLTIGPVKIPNVVTSLSLQKHGAFATPAYQGNIG
                     GGILKRFVVTFDYANHVMYLKPLPKPVADLDTYDRSGMWINAVGAGMQVMDVTADGPA
                     AQAGIKPGDVITQVNGKPATTIPVYELRRMLRDEAPGSVVHFGIQRDAQTRHAAVTLR
                     DQI"
     gene            11315..13117
                     /locus_tag="VFK00_02335"
     CDS             11315..13117
                     /locus_tag="VFK00_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET7591826.1"
                     /translation="MALACILAAPLAAAASVRTWINPLDIDYRYNYEQMKQGISYRTG
                     ADPAFVRYEGAYYLFLTLADGYWHSPDLVHWTFVKPDRWPFDGPVAPATLVADGKLFL
                     MQSAMQPRPLLYSTDPAHGHWDFWTRLLPPVPGAVWAGHEKDMKPGDLPPGPWDPGLF
                     QDDGGKVYLYWDSSNVFPIYGAQLDLAFDKQAEGEGKRVKFVGKPLALLRADPASHGW
                     ERFGQDHGDTTIAPYIEGAWMSKHDGRYYLQYGAPGTEYNVYATGVYVGNAPLGPFTY
                     APYNPVGYKPGGFVVGAGHGSTFQDAYGNWWNSGTMWVGVNWRFERRVDLFPGGFTRD
                     GQMWVDTRFGDFPQRMPDHKLKPGESTFAGWMLLSYRKAATASSQLPGYPASNTADEN
                     PRTFWVAANDNAGQTLTLDLGGPRTVRAVQVNYADYQSGRYGDAQDIVTQFRLEGSLD
                     GKRWTTIADLSHSTQDHANAYVELDRPARIRYVRYVHVHVGAQHLAISDLRVFGNADG
                     PPPPAPELVSATRLPDARDAEVAWKPVKGAVGYNVRWGIAPDRLYETWQRFGDQPTML
                     KLTALNKGVRYFVAVEAFDERGVSKLSRTLVLEP"
     gene            complement(13151..15898)
                     /gene="aceE"
                     /locus_tag="VFK00_02340"
     CDS             complement(13151..15898)
                     /gene="aceE"
                     /locus_tag="VFK00_02340"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET7591827.1"
                     /translation="MNAVAQEFMDQDPDPRETREWIESIEAVISAEGTERAHQILEKL
                     VDETRRAGGHLPFSTTTDYVNTIPPQQEAKSPGDAAMEWRIRCLIRWNAMAMVVRANR
                     KPGSLGGHIASFASSATLYDVGFNHFWRAPSEDHPGDLICYQGHASPGIYARSFLEGR
                     LSTDDLDHLRIETQPGVKGLPSYPHPWLMPDYWQVPTVSMGLGPIQAIYQAQFMKYLE
                     ARGMVPKSDRKVWCFLGDGECDEPESLGAISVAGREHLDNLVFVINCNLQRLDGPVRG
                     NGKIIQELEGVFRGAGWNVIKLIWGSYWDPLLARDSKGVLRKLMMETVDGEYQACKAF
                     GGAYTREHFFGKYPETAAMVANLSDEDIWRLNRGGHDPHKVYAAYHAAVQTEGMPTVI
                     LAKTVKGYGMGAAGESLNIAHQAKKMHQDAVKIFRDRFQIPISDKQLEGEDLESVPYY
                     HPGKDSPEVQYMQERRKELGGPLPYRRAKSTKTLPVPAIDKFEQITKGTGDREISTTM
                     AMVRGMNLLLRDKEIAPHVVPIVVDEARTFGMEGMFRQIGIYAPGGQKYRPQDADQLL
                     YYREAEDGQVLQEGISEAGGVSAWIAAGTSYSVSDQPMLPFFIFYSCFGFRRIGDLIW
                     AAGDQRTRGFLLGATAGRSFAGEGLQHADGDSMLVAGVVPNCKAYDPTYSYETAVILQ
                     DGIRRMLHEQEDVFYYVTVMNENYVHPDMPKGCEEGIIKGMYLLKAGNGEQGTGKGKS
                     KGKSGSKAARVQLLACGVVLREAITAAELLEQEFGVQADVWSCPSFVELSRDGFDCER
                     WNRLHPDPKERRVPYVEQCLDGHDGPVIVASDYVRAFGDQVRAFVPHGRRCTVLGTDG
                     YGRSDSRAHLLEFFEVNHHWIAHAAVSALADDGVLARKDVERAIKLWNLDPDKPNPLW
                     A"
     gene            complement(15895..>17985)
                     /locus_tag="VFK00_02345"
     CDS             complement(15895..>17985)
                     /locus_tag="VFK00_02345"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7591828.1"
                     /translation="FRKFAGIDVFDIEIDERDPDKLVEIIASLEPTFGGINLEDIKAP
                     ECFQVERKLRERMNIPVFHDDQHGTAIIVGAALTNALIVVGKKIGEVKVAASGAGAAG
                     IACLDMLVRLGVKPENIRVCDKDGVLYHDRPGLDRSLERYARKTKARTLREIVKGADV
                     FLGVSAGGVLTRDMVATMAARPVILALANPTPEITPEEARAAKPDAVIATGRSDYPNQ
                     VNNALCFPYIFRGALDVGATGINEQMTVACVHAIAQLARREASDVAQRAYGGRVPHFG
                     RDYLIPRPFDPRLLVQVAPAVARAAMESGVATRPLTDMRAYTEKLTQFVFRTGLAMKP
                     VFERARAKPMRVAYADGEEETILRAVQVLVDEGLVKPILIGRPDVIARRIDRIGLRLE
                     QGRDFELVNLHSDPRFDAYWQHYYELMQRRGVTPSLAKSVVRSRSTVIAALMVARGDA
                     DALICGTVGRYQRKAEYIRDIIGLDAGVATLSSMAAVANDKGLSFFLDTHMQHDPSPE
                     QIAEATLQATLRLKLLGVVPRVALIAASNFGARNTPTARKMQEVLRLLRERDPSLEVE
                     GEMQADVALDPELRARVFPNSRLTGRANVFVFPNLAAANAAFNITRSMSDGVVIGPIL
                     MGVAKPAHVLTPQATVRRVVNMSAIACVEAQIRAPHERVVKRKARAKAQGKAPKSSGT
                     IKRSARSAAHGSKR"
BASE COUNT         2864 a         5936 c         6150 g         3035 t
ORIGIN      
        1 cgcgacgcgg gtggcgacgc cgaggtcgcg gcattcgatg tcaccgatgc ggaggccgca
       61 cgcgccgcga tcgacgagct gttggagtcg ggtccgatcc atgcgctggt ccacaacgcg
      121 ggcatccacg acgatgcgcc cctggccggg atgaacgcgg atcagtggcg gcgcgtgatc
      181 gacgtgtcgc tcaatggctt cttccatgtc acccagccgc tgctgctgcc gatggcccgc
      241 gcgcgcttcg ggcgaatcgt tgcggtttcg tccattgccg cgacgatggg caaccgcggc
      301 caggtcaact acgccgcggc caaggcggcg ctgcacggcg ccgcgaagtc gctggcgcgc
      361 gagatggctt cgcgcaacgt caccgtgaac gtggtggcgc ccggggtcat cgaggggcgg
      421 atgacccagg cgctgttcac cgaagatcag atcaaggcga tgatccccgc cgcgcgcacc
      481 ggcacgcccg cggaagtcgc gggcgtggtc gcattcctgt gctccgatgc tgcgtcctac
      541 gtgaacgggc aggtcatcgg cgtgaacggc ggcatggcat agggcggagc ccgcgaagcg
      601 gcttccgccg ggccagcttc gagacgcatg cggcggaagt ggctgcgccg gtccgcccta
      661 cgcggcggcg cggaacagcg tcagcggctc gcgcggaacg acgggcaagc cccccaggtc
      721 catcgtgggc atctcgctgc gggcgcgcgc cgcaagcgtg ccggcaaggt tgtggaggag
      781 gtcgatgttg gcgcgcagcc gcgcccggat catatggtcg tccaggtcct cgccaaggcg
      841 cgcgttcaac tgcgcgaacc aagccaggtc ctgctggtcc atccagggcg gcgccggatg
      901 tgcgggattc gcacggtgcc acgtgcggaa gaactcctgc atccgcaggt tgagcgattc
      961 ggcgtcggcc agttccgtcg cgaggtcgcc gaacagggcg aagtcgtcca ggcgaccgcc
     1021 gatcacgagc gggcacagta cgccccagta atacgcgtag tcccacagca ccttggcggt
     1081 catcacccgc gcgtcgccga acagctcgta ctggccgagg tagaggttca gcatgttgcg
     1141 gtagaacgag aagaacatct gctggcagcg ccgtaccggt ttcgcgatcg gttcgccggc
     1201 gaaatcgcgc gcgaccaggc cggtaatgta ggtgttggcg atcgcgatga aatcggttcc
     1261 gggcgaatag aagggatcga gaaacattcc ggcctcgccg gtcatcgccc aacgatcggc
     1321 gctgaacacc tggcggcagc cgtaggagac ttcgcgcagg aaccggaagt cgagcagcgc
     1381 gtcgcgctgg tcttccaggc gcccggcgag cgcgggctgg tggacgcgca accaggcgag
     1441 tgcgcgctcg aaggtgtcga acgattcgac cgggtgccgg gtggtgtcgg cgacgatgcc
     1501 gaccgaatgc gccccggagg cgagcgggat cagccagacc cagtagcccg ctccgcacag
     1561 gtggttggtg gaacgccagc gttcctgcgg ttcgcacaac ccgcgccacg cagggtcttc
     1621 gcaccagtcg tccagcttca ggcgcgcctc gacccggaac catgcggcgc cgatgcggtg
     1681 gccgttgtct tcgctcaagt ccagcttgcg gcggagcagt ccggcacggc ctgaagcgtc
     1741 gaccagccag cgcgcgcgat gctcggcatc accggccttg accgcgtgca tcgcgccatc
     1801 gccgccgggt tcgatcgacc gcacgctcgc gccttgctcg atcgcgatgc cgcgctcgcg
     1861 cgcgcgcgtc gccaggaaat tctccagcgt gccacgctcg acctgccatg acggcgtcgg
     1921 gaacacgcga ctgatgccga gttccggaac ctggtccagg tgggcgcagc cgtcgctgaa
     1981 gaagaaccgg aacccgaatt tgcggacctg cgcgccgtcc aggtgctcgc gcaggccgag
     2041 cacgtcggcg aaatagtgcg cgccgatttc caccgtggat tcgcccacct tgaacgcggc
     2101 ggcgggcgcg gggccattgc gccgttcgaa cactcggatc gacagttgcg ggcagcgctc
     2161 gcgcagttgc agtgcgagcg tgaggccggc cagtccgccg ccgacgatca gcacatcgct
     2221 cgtcgtggcg gcggccatgc gatcagctcc gcgctggcgg cgggctgccg acgcggcgga
     2281 gcggcgccga cgtctcggct tcgagccggt ggtcggggtc gtacacctcg ggcgcgctgc
     2341 cgtgcgaggc gcggtagctc tgcaccacgc tgccgaagtg aacgatctgt ccaatcgtgt
     2401 accaggtgat ccgcaccacg tcgcgcaccg gccggaaatg gctgtgccgg gcgctgccgt
     2461 cgtaccggca ggcgatcggg atcgagacga ccggcatgtc caggtcgcgc gacgcggtga
     2521 tcaggatggc cgcctcgaac acgaagtcct gcgcgggaag gtcgaccagt tctacggccg
     2581 cgcgcggata ccagcgttgc ccgctttggg tgtcggcaac gggacggccg cagccccaac
     2641 tgattcccca gtccgcgacg gcgttggcgc ggcggcgggc attcggctgg cactcccgtc
     2701 ccagcatccg cgcgccgatg acgagcgctc cggggagccg ccgggccgcg gcgaggatac
     2761 gcgggatgtc ggcgggatcg tgctggccgt caccgtccat cgtgaggacg ccgccggcgc
     2821 cgagttccag cgccttgcgg aatcccgcgc gcaaggcttc gcccttgccg cgccgcgtgt
     2881 cgtggcgtat cagcgtcacc ggcaaggccg cggcgatgtc cgcggtgcgg tcggttgacc
     2941 cgtcgtcgat cacgatcacg tccgcgcagt gcgccagcac gccagccagc aattcgccga
     3001 tggcctgttc ctcgttgagg caggggacga cgatgaaagg ccggtcggtg gcgtcgctca
     3061 aggcgtgatc tccatgtcca gaaacagtcc gggcgacagt ggcaacgcca gcctggtcga
     3121 ttcccggccg gccagcgcgg ccagcagcgc cagcgcatgc gcgttggcag gattgtcgcc
     3181 tgccaccgcc cgcaacgcca gttccacggg cgggaggggg ccggattgcg cgtcgcgcag
     3241 cgaccagtcc agccgcgcgc cgcccggcac cacgggcgtc aatacgagtg ccatgccgaa
     3301 ggcaagggtg gtccgggtca tctccgcgag tggaccggtc gaagccacgt cgcagcaggc
     3361 gaacaacact ggtgcctgct cggtgcaggc gaaggccgcg gcctcgagca ggccggcggc
     3421 aaagctgtag tggtgcgcgg tcaccgcacc ggagggcgcg gtgcagccgg tggcgatggt
     3481 ccagtagccc gccggtgcgt tcagcaccga gttgtggaat ttggtaggcg acagttcctg
     3541 cggcgcgctg gcgagggttt cgcacacgta atcggttatc gcgagttcgc cgtgggtgga
     3601 ggcgaacacg cagggaagcg cggacggatc gcattgcgcc atggcgcagg cctgctgcgc
     3661 gacctcgacc gcgagccgca cgccatccgg cgcgcgccgg cgttccgctg ccggcaatgg
     3721 atccgctgcc ggcttggcag gcgcgtccgc atcgaggtcg tgttcgccgc gcaagatgct
     3781 gcgcagggat ggccagtcgg gcgcgccccg gatccatgcg ccgatgccgg tgaccgccag
     3841 cgtccgtgcc ttgctcatgc cgcgggcccc cgcgcgaaca cgaggcaggc attgttgccg
     3901 ccgaacccga aggagttgct gagcgcgatg tcgatgtcgc gggtctcggg atccagggcc
     3961 agccacggct cggcttccgg caaggcgtcg gtgcagccga gatcgggcgg aaccacgctg
     4021 tgttcgatgg cgagcaacgc gatcaccgct tccagtgcgc cggcagcgcc cagcgtgtgg
     4081 ccggtgaagc ccttggtcga gctggcgcgg gtccgcggag caaaacgcgg cagcagcttc
     4141 gcctcgacct cgtcgttctt caccgtggcg gtgccgtgca ggttgacgta gtccacttgc
     4201 tccgccgtga tgccggcgcg cgccagcgcg tcgtccagcg cggccagggc accgcgggct
     4261 tccgggtgcg gcgtggacat gtgccaggcg tcggacgact cgccgtaccc gatcaggcgc
     4321 ggcgcgcccg gcgcatcttc gacgcgttcc agcagcgcga acgccgcggc ctcgccgatc
     4381 gagatgccgt cgcgctgccg atcgaacgga cggcacggtt gccgtgaaac gagttccagc
     4441 gaatggaagc cgaacagcac gctgtcgcac aggctgtcga cgccgcccac cagcgccgcg
     4501 tcggcgaggc cgaggcggat cagccgttcg gcggaagcga acaccttggc cgacgacgag
     4561 cacgccgtcg aaatcgtcag cgtcggcccc gcgatgccgg tggcgtcggc gacgaacgcc
     4621 gccagcgagt gcggggtgtg gatttcgggg cggcgcaggt cggatggaaa gcggccgtcc
     4681 tcgagccgcc ggtaggcgac ctcggtggcc tcgatgctgg cggtcgaggt gccgaggacc
     4741 agtgccacgc gcttcgggcc atgccgcgcg acggcggcgc cgatggcatc gagcatgccg
     4801 tcgtgctgca ggccgagcca ggcgaggcgg ttgttgcggc agtcccagtg cgtgtggcgg
     4861 gcaggcaatg ccgcctcctc gacaccttcg acgcgtccga tccagcagtc gagcggcgcg
     4921 caggaaaaat cgttccggcg caatccgcgc cggccctcgg cgagcgcgcc caaatgggcg
     4981 gccgttccgc gccccagcgc ggacgtcgcg gtgcggacgg tgatggcgag cggctcgatg
     5041 tgtggtggca tccgttgcac tgcggttgag cgggtgggcc agtataagca gcgcgcggcc
     5101 gttgccgcag gcgcggtgtc gtacactgcg cggcgcggcg atcggccgcg tgttgcacat
     5161 ggaggggatc atggtgtgtt tcgaaaaccc gaagcgttgg ttgccggtgc tgctggcgcc
     5221 ggccctgctg ttgctgatgg cgttccgtca ggttccgttg gtcgacccgg acccgatcgc
     5281 ggtgccgcac aacgtcaccc aggcgcaagt cgaacaggtc atcggccgtg ccctgaccgc
     5341 caggaactgg cgcatcgtca agcacacgcc gggcgaaatc gacgccgtgt acgacccgcg
     5401 tgacttcagc gtcaccatcg ctgtgcatta cgacgcgcaa cggatccaga tcaactacgt
     5461 caccagtgac cacctgcgct acgaggtgaa aaatggcgtt cgttacatcc ataccaacta
     5521 cgcaagctgg atcaagaatc tcgtcaccaa tatctccaat gggctgatga tggcgagctg
     5581 agaacggcgg gcggcggatt cgccgcccgt ctccggaaaa cttcatgcaa gcagttctgg
     5641 aaaccgtgga tggattcctg cgtgacttgc aggaccgcat ttgcagtgcg ctggagcgcg
     5701 tcgatggcgg caggcgattc gtcgaggacg catggacgcg cacgagcggg ggcggaggcc
     5761 gcacccgggt cctgaaggac gggacggtat tcgagcaggc gggggtcaat tactcgcgcg
     5821 tcaccggcga caccttgccg gcatcggcga cggcgcatcg gccggagctc gcggggcgct
     5881 cgtggaccgc caccggggtg tcgctggtgg tccatccgaa gaatccgtat gtcccgaccg
     5941 cgcacgccaa cgtgcgctac ttcgaagccg acaaggaagg cgaggcaccg gtgtggtggt
     6001 tcggcggcgg cttcgacctc acgccgttct acccgttcga cgaggattgc gtgcactggc
     6061 atacggtcgc gcacgacatc tgcgcaccgt tcggcgacga ggtgtatccg cgctacaaga
     6121 cgtggtgcga cgagtatttc tacctgaagc accgcggcga aacccgcggt atcggcgggt
     6181 tgttcttcga cgatttgaac gactgggagt tcgagcgctg ctttggattc atgcaagccg
     6241 tcggcaacgg gttcctcgat gcctacttgc cggtcgtcga acgccgcaag gacgtgccgc
     6301 atggtgagcg cgaacgcgag ttccagttgt accgccgcgg acgctacgtc gaattcaacc
     6361 tggtgtacga ccggggcacg ctgttcggcc tgcagagcgg cgggcgcacc gagtcgatcc
     6421 tgatgtcgct gccgccgcgg gtgcgctacg aatacggcta tgcccccgaa cctggcagtg
     6481 ccgaggcgcg cctcgcggaa tacctgaagc cgagagagtg ggtgtaggtt gggctgagcg
     6541 cttcttgcga agcccaacgg ggggcgcgaa aacgttgggc ttccgccgat ggagccggcg
     6601 tcaacccaac ctacattccc ggtgcggaac ccttgggccg cggaatgccg tgcttgctcg
     6661 acgaagcctt tgtaggttgg gttgagcgct tcttgcgaag cccaacgggg ggcgcgaaaa
     6721 cgttgggcgt ccgccgatga agccggcgtc aacccaacct acattccctg tgcggaaccc
     6781 ttgggccgcg ggacgccgtg cttgctcgac gaagcctttg taggttgggc tgagcgcttc
     6841 ttgcgaagcc caacgggggc gggaaaacgt tgggcttccg ccgatggagc cggcgtcacc
     6901 ccaacctact cccatcgatt ggcgtttcca gcgaagccgg ggcgctgcac acgcacgacg
     6961 cagaagcgtt gcccgctggg cgcctgcatc accgtccagc gctccagctt tttgagaacg
     7021 gctgcgccaa gcttttccag cctcgcgact tccgcaccga tgtcgtcggt ttcgatgtcg
     7081 aggtggacgc ggcttgcgtg gtccacgcgc tggagctgca ccatcggctc gtccggcagc
     7141 gcggcgagtt gccggtagtt cccgcggctg ccgggatgat ccatgtccac cggccgcccg
     7201 agtgcggcgg cccagaagcg ggccgccgcg tcgatgtcgg cgacgttgca gtcgatctgc
     7261 atggagcaaa gccgcgaatg atgcatctca gtggcccatc atcttctcgc ggcgcgcccg
     7321 gaactcgctg tcctagtacc actccggcca ttgatcgctg agcgagagct cgcggcccac
     7381 ctcgtaaagg gcctgcgtgt ccgcggcgag ccccgagaaa tcccaggccg gatcgaaatt
     7441 gtcgagcggc gtgtggtagc ggtgcgcggt gtaatcggcg agcgcagccg tgcccgcccc
     7501 ggtgccacca tcgaccttgt cgttgccggt ctgggtcatc agcgcgggca cgccggcctt
     7561 ggcgaagctg aagtggtcgg agcggtaata gaagccgttc tggggtgtct ggtcggatat
     7621 cgcgtagcgt ccctgtttct tcaacacggc tttcaacatg tcttccagtt gcgactggcc
     7681 gctgccgatg atctgcaggt ttttcgtggg accgatcacg tccatcgcgt ccatgttgat
     7741 gtccgcgacg gtcttgtcca tcggaaacac cgggtgcctg gtgtagtaca gcgaaccgag
     7801 caggccggac tcttccagcg tcacggcggc aaacagcacg ctgcgctgcg gtgcgggatc
     7861 ctgcgccttg aatgccttgg cgatttccag aagtgccgag attcccgtgc cgttgtcgac
     7921 cgcaccgttg tagatcttgt cgcccttcag gctgtcgtcc tcgccgaggt gatcccagtg
     7981 cgcggaatac acgaccactt cgtcgggacg cgcggtgccc ttgatcattc cgagcacatt
     8041 gttggccgaa ccatggtcga tcttgctgtc cagcgtgatc gatgcggtcg ccttcaaagg
     8101 caccggcttg aagccgcggt ggtcggcctc ggccgccagc ttgtcgaaat cgaggccggc
     8161 tttcgcgaac aggcgctgcg cggcgtcccg ggtgatccag cccgccaccg gcaggcgcgg
     8221 ggcgggatct tcgcttggcg gcaaggcgag ctgcggtccg ccccaactgt tcatcaccac
     8281 cgaccacgga taccccgccg cgtcgttgga ggtgtgcacg atcaggcaca tcgccgcgcc
     8341 ctggcgtgcg gcttcctcgt acttgtaggt ccagcggccg taataggtca tggccttgcc
     8401 cttgaagagg tccggatcgt ggtcgccgaa gccgggatcg ttcaccagca acaccacggt
     8461 cttgcctttg acgtcgagac ccttgtagtc gttccagtcc gattcgggcg cattgacgcc
     8521 gtagccgacg aacacgatgc cggaatcctt gatcgccacg tgcggtttgg cctgcagcgt
     8581 catcgcgacc atgtccttgc cgaaatcgaa cgcctgggtg ccgccgccct cggcgacatc
     8641 cagcttgacc ttgccgggcg actcgagcgt ggtcgacacc gtgggcacct tctggaacca
     8701 cgagccgtgg ttgccgggct cgaggccgag ttgcttgaac tgctcgacga tccaccgggt
     8761 ggcgcgctcg ccgccgggcg tgccgggttt gcggccgtcc atggcgtccg acgaaatcgc
     8821 ctggtcgtag gcgcggaagc cttgcgcggt gatcgcggcc gagagcggct gactggcgga
     8881 tgctgcggtg ccgtctgcgc gtgtcggcgc ggttgccgta ttgccctttt cggcaggtgc
     8941 aggcgcgccg ttgccgttgc cagcgggcga accgcacgcg gcggcgatgc aggcgacggc
     9001 ggccaggacg gtgaggatgc tgcgacgcat ggcgaactcc tccgacgaag aaatcgtgtc
     9061 gagtgtaccc cgtgcatggt ccgcgcaagt tcatgtgccg gtcgagcttg acgacatgac
     9121 gagtgtcatc catagcgtcg ttaccacaag cacatgcgcg cgcgatacct gcgggccgac
     9181 aatggcggcg acccgatcaa gcgccgtcca gccgccctcg gcacgcaaac gggttgtggg
     9241 atcggcgcgc ggtcgcccga cgagggcgtg tggctgcgcg ccgatctcca cacccaccgc
     9301 cgtggcaaag gccgtcgacg ggttgcccgc gatgggtacc gttcttcatt cgatccgagg
     9361 tggaccatgc atcgttgctt cgtttccctg gcgggcttgg cgctgctggt atcgttcgga
     9421 acggcacatg ccggcacaga tccagccgcg gttctccaag cgtaccgcgc tgcgacgggg
     9481 ggtgacgcgt ggaacggcaa ggccgtgatg aagactgtcg cgaacctgtc cggacagggg
     9541 ctgaccggca cggatacgtc gaccgcggat ctgcgcaccg gctactcgat cgaccactac
     9601 acgatgggac ctgccagcgg ggccaacggc ttcgatggcg agcacgtctg ggagcagggg
     9661 ccggatggcg cggtcaacat cgagcagggc ggcgatgccc tgccgcgcgc gatcgactcg
     9721 gcataccaga ccgcgaacct gtggtggcgc ccggacttcg gcggcgccaa ggtcgagcct
     9781 gccggcgaca agccgtgcgg cacctcgacc tgcgcggtgc ttgaggtcac gcccaagggt
     9841 ggcctgccgt tccaggcatg gttcgacacc ggctcgcacc tgctggtgcg cacctggcag
     9901 cgcatcggcg gccagacgac caccatcgat ttctccgatt accgcggcgt cgagggcgtg
     9961 cgcgtcccgc acaaaatcgt gatcgacaac ggacacggca agcagtacct gctgaccgcg
    10021 acggtttccg cggtggcgtt cctgccggcg cagcctgttg cgacatacgc gccaccgaaa
    10081 tcggagatga aaaacatctc catcgccggt ggcgctgccg agacgacctt tccgttccgc
    10141 ttgatcaaca accacatcta cacccatgcc tgggtcaacg gacagggtcc gttgctgttc
    10201 atcgtcgata ccggcggcca caacatcctg gtgccgtcca ccgccagggc gctcggcatc
    10261 aagatccagg gcgcgatggc tgccacgggc gcgggcaaca aggccatgga ctacgggctg
    10321 gcgcagatcg cctcgctcaa ggtcggcgac gcgaccttca gcgaccaggt cgtcggcgtg
    10381 ctcgatttcg agccgaacgg cgtggaaggc gtggacatca agggcatggt ggggttcacc
    10441 gcgttcaagc gtttcgtgac gcggatcgac tacggcaagc acgagatgac gctgatcgaa
    10501 ccggatcatt tcgatccgca ggacgccggt acgccgattc cgttcgtgtt caacggcgat
    10561 attcccgaag tcgagggcac attcgaaggg attcccggca agttcgacat cgacaccggt
    10621 tcacgcagtg cgttgacgct gaccggcccc ttcgcgcaag aaaacaacct gcgctccagg
    10681 catcccaagg gcgtgaccgc ggtggatggc tggggcgtgg gcggggcggc gcgcggctac
    10741 gtcacgcgcg gcaagctgct gacgatcggg ccggtgaaga tcccgaacgt ggtgacgtcg
    10801 ctgtcgctgc aaaagcacgg cgcgtttgcg acgcccgcgt accagggaaa catcggcggc
    10861 gggatcctga agcgcttcgt ggtgaccttc gattacgcca accacgtgat gtacctgaag
    10921 ccgctgccga agcccgtggc cgacctcgac acctacgacc gttcgggcat gtggatcaat
    10981 gccgtcggcg ccggcatgca ggtgatggac gtgaccgccg acggcccggc cgcgcaggcg
    11041 ggcatcaagc cgggcgatgt gatcacgcag gtgaacggga aaccagcgac gacgatccct
    11101 gtctacgaac tgcgccgcat gttgcgcgac gaagcgcccg gcagcgtggt gcacttcggc
    11161 atccagcggg acgcgcaaac gcgccacgcc gccgtcacgt tgcgcgacca gatctagcgt
    11221 cgtccgcgcc acgcttgcat cggtgcgggt gtcgccacat gcttgccgca tgtggacgaa
    11281 cccgcaccct gcacggctca tctctgtttg cggcttggcg ctcgcctgca tactcgcggc
    11341 accgctcgcc gcggcagcct ccgtccgcac ctggatcaat ccgctcgaca tcgactatcg
    11401 ctacaactac gagcagatga agcagggcat ctcgtaccgc actggcgcgg accccgcgtt
    11461 cgtgcgctac gagggtgcct attacctgtt cctgaccctg gccgacggtt actggcattc
    11521 gcccgatctc gtccattgga cgttcgtgaa gccggaccgc tggccgttcg atggcccggt
    11581 tgcgccggcg acgctggtcg ccgacggcaa gttgttcctg atgcagtccg cgatgcagcc
    11641 gcggccgttg ctgtattcca ccgatccggc gcacggccac tgggatttct ggacccgcct
    11701 gctgccgccg gtgccgggtg cggtatgggc cgggcacgaa aaagacatga aaccgggcga
    11761 cctgccaccg ggaccgtggg atccgggcct gttccaggac gacggcggga aggtctatct
    11821 gtactgggac tcgtccaacg tgttccccat ctacggcgcg caactcgatt tggcgttcga
    11881 caagcaggca gagggcgaag gcaagcgcgt gaagttcgtc ggcaagccgc tcgcactgct
    11941 gcgtgccgat ccggccagcc acggctggga acgcttcggg caggaccacg gcgacaccac
    12001 catcgcgccc tacatcgaag gcgcgtggat gagcaagcac gacggccgct actacctcca
    12061 gtacggcgca cccggcaccg agtacaacgt ctatgccacc ggtgtgtacg tcggcaacgc
    12121 accgctgggg ccgttcacct acgccccgta caacccggtc ggctacaaac ccgggggctt
    12181 cgtggtcggc gcgggccacg gctccacctt ccaggatgcg tatggcaact ggtggaacag
    12241 cggcacgatg tgggtgggcg tcaactggcg attcgaacgg cgcgtcgatc tgtttcccgg
    12301 cggcttcacc cgggacgggc agatgtgggt cgatacgcgt ttcggcgatt ttccgcagcg
    12361 gatgcccgac cacaagctca agcccggcga aagcaccttc gccggctgga tgctgctgtc
    12421 gtaccgcaag gccgcgacgg cgtcctcgca attgccgggt tatccggcct cgaacacggc
    12481 cgacgagaat ccgcgcacgt tctgggtcgc ggcgaacgac aacgccggac agacgctgac
    12541 gctcgatctc ggcggccccc gcacggtgcg cgcggtgcag gtcaattacg ccgattacca
    12601 atccggccgc tacggcgacg cacaggacat cgtcacgcaa ttccggctcg agggttcgct
    12661 cgacggcaag cgctggacga cgatcgccga tctttcgcat tcgacccaag accacgccaa
    12721 cgcctatgtc gaactcgacc ggccggcgcg catccgctac gtccgctacg tgcacgtgca
    12781 cgtcggcgca cagcacctgg cgatctccga tttgcgcgtg ttcggcaacg cggacggtcc
    12841 gccgccgcct gcgccagagc tggtgtcggc cacgcgcctg cccgacgcgc gcgatgccga
    12901 ggtcgcgtgg aagccggtga agggcgcggt cggctacaac gtgcgctggg gcatcgcgcc
    12961 cgaccgtctt tacgaaactt ggcagcgctt cggggaccag ccgaccatgt tgaagctcac
    13021 cgcgctcaac aagggcgtgc gctacttcgt cgcggtcgag gccttcgacg agcgcggtgt
    13081 ctcgaaactc tcgcgcacgc tggtactcga gccctgatcc gccgcggtcg aaccagggcg
    13141 caagccggcg ttacgcccag agcggattcg gcttgtccgg atcgaggttc cagagcttga
    13201 tcgcgcgctc gacgtccttg cgcgccagca cgccgtcgtc ggccagcgcc gagaccgcgg
    13261 cgtgcgcgat ccagtggtgg ttgacctcga agaactcgag caggtgcgcg cggctgtcgc
    13321 tgcgcccgta gccgtcggtg ccgagcacgg tgcagcggcg gccgtgcggc acgaacgcgc
    13381 ggacctggtc gccgaaggcg cgcacgtagt cggacgcgac gatcaccggg ccgtcgtggc
    13441 cgtcgaggca ctgctcgacg tacggaacgc gccgctcctt cgggtccgga tgcagccggt
    13501 tccagcgctc gcagtcgaag ccgtcgcggg aaagctcgac gaagctcggg cacgaccaca
    13561 cgtccgcctg cacgccgaac tcctgctcca gcaattccgc ggctgtgatc gcctcgcgca
    13621 gcaccacgcc gcacgccagc aactgcaccc gcgccgcctt gctcccgctc ttgcccttgc
    13681 tcttgccttt cccggttccc tgttccccgt tccccgcctt caacaaatac atccccttga
    13741 tgatcccttc ctcgcagccc ttcggcatgt ccggatggac gtagttctcg ttcatcacgg
    13801 tgacgtagta gaagacgtct tcctgctcgt ggagcatccg gcggatcccg tcctgcagga
    13861 tcaccgcggt ctcgtaggag taggtcgggt cgtacgcctt gcagttcggg accacgcccg
    13921 cgaccagcat cgaatcgccg tcggcgtgct gcaggccttc gcccgcgaag ctgcgtccgg
    13981 cggtcgcgcc cagcaggaaa ccgcgggtgc gctgatcgcc ggcagcccag atcaggtcgc
    14041 cgatgcggcg gaaaccgaag cacgaataga agatgaagaa cggcagcatc ggctggtcgg
    14101 ataccgagta gctggtgccg gccgcgatcc aggccgacac gccgcccgct tcagagatgc
    14161 cttcctgcag cacctggccg tcttcggctt cgcggtagta cagcaactgg tcggcatcct
    14221 gcggacggta tttctggccg cccggcgcat agatgccgat ctggcggaac atgccctcca
    14281 tgccgaaggt gcgggcctcg tcgaccacga tgggcacgac gtgcggcgcg atttccttgt
    14341 cgcgcaacag caggttcatg ccgcgcacca tcgccatcgt ggtgctgatc tcgcggtcac
    14401 ccgtgccctt ggtgatctgt tcgaacttgt cgatggccgg caccggaagg gtcttggtcg
    14461 actttgcgcg ccggtacggc aggggaccgc cgagttcctt gcgacgctcc tgcatgtact
    14521 ggacttccgg cgaatccttg ccggggtggt aatacggcac cgactcgagg tcttcgccct
    14581 cgagctgctt gtccgaaatc gggatctgga aacggtcgcg gaagatcttc accgcgtcct
    14641 ggtgcatctt cttggcctgg tgggcgatgt tgagcgattc gcccgccgcg cccatgccgt
    14701 agcccttgac cgtcttggcg aggatcacgg tcggcatgcc ttcggtctgc accgccgcat
    14761 ggtaggcggc gtacaccttg tgcggatcgt ggccgccacg gttcagtctc cagatgtcct
    14821 cgtcggaaag gttggcgacc atcgccgcgg tttccggata cttgccgaag aaatgttcgc
    14881 gcgtgtacgc gccgccgaac gccttgcaag cctggtattc gccgtccacg gtttccatca
    14941 tcagtttccg cagcacgccc ttgctgtcgc gggcgaggag cggatcccag tacgagcccc
    15001 agatcagctt gatgacgttc cagccggcgc cgcggaacac gccttcgagt tcctggatga
    15061 tcttgccatt gccgcgcacc ggcccatcga ggcgctgcag gttgcagttg atcacgaaca
    15121 ccaggttgtc gaggtgttcg cggcccgcga ccgaaatcgc gccgagggat tcgggttcgt
    15181 cgcactcgcc gtcaccgagg aagcaccaga ccttgcggtc tgacttcggc accatgccgc
    15241 gcgcttccag gtacttcatg aactgcgcct ggtagatcgc ctggatgggc ccgaggccca
    15301 tcgacaccgt cggcacctgc cagtaatcgg gcatcagcca cggatgcgga taggacggca
    15361 ggcccttgac gcccggctgg gtctcgatgc ggaggtgatc gaggtcgtcg gtactcaggc
    15421 gtccttccag gaacgagcgc gcatagatac ccgggctggc atgcccctgg tagcagatca
    15481 ggtcgccggg gtggtcttcc gaaggcgcgc gccagaagtg gttgaagccg acgtcgtaca
    15541 gcgtcgccga tgatgcgaag ctcgcgatgt ggccgcccag cgaccccggc ttgcggttcg
    15601 cgcgcaccac catcgccata gcgttccagc ggatcaggca gcggatccgc cactccatcg
    15661 cggcgtcgcc gggcgacttg gcttcctgct gcggcggaat ggtgttcacg tagtcggtgg
    15721 tggtcgagaa cggcaggtgg ccgccggcgc gccgggtttc atcgaccagc ttttccagga
    15781 tctggtgcgc gcgctcggtg ccctcggcgc tgattaccgc ctcgatcgat tcgatccact
    15841 cgcgggtttc gcgcggatcg gggtcctggt ccatgaattc ctgtgccact gcgttcatcg
    15901 tttgctcccg tgcgcggcgc tccgcgccga tctcttgatg gttccggatg atttgggtgc
    15961 cttcccctgc gccttggcgc gggccttgcg cttgaccacg cgttcgtgcg gcgcgcggat
    16021 ctgcgcctcg acgcaggcga tcgcgctcat gttcacgacc cgccgcacgg tcgcctgcgg
    16081 ggtcagcacg tgtgccggtt tcgccacgcc catcaggatc gggccgatca cgacgccgtc
    16141 cgacatcgag cgggtgatgt tgaacgcagc gttggcggcg gcgaggttgg ggaacacgaa
    16201 tacgttggcg cggccggtca ggcgcgaatt cggaaaaacc cgtgcccgca gttccggatc
    16261 gagcgcgacg tcggcctgca tctcgccttc gacctcgagg cttggatcgc gttcccggag
    16321 cagccgcagc acttcctgca tcttgcgtgc ggtcggcgtg ttgcgggcac cgaagttgga
    16381 cgccgcgatc agcgcgaccc gcggcaccac gcccagcagc ttcaggcgca gcgtcgcctg
    16441 cagcgtcgcc tcggcgatct gctcgggcga cgggtcatgc tgcatgtggg tgtcgaggaa
    16501 gaacgacagc cccttgtcgt tggcgaccgc ggccatcgac gagagcgtcg cgacgccggc
    16561 atcgagtccg atgatgtcgc gtatgtattc ggccttgcgc tggtagcggc cgacggtgcc
    16621 gcagatcagt gcgtcggcat cgccgcgcgc gaccatcagc gccgcgatca ctgtcgagcg
    16681 cgagcgcacc acggacttgg ccagcgaagg cgtgacgccg cgccgctgca tcagctcgta
    16741 gtagtgctgc cagtaggcat cgaagcgcgg atcggagtgc aggttcacca gctcgaaatc
    16801 gcggccttgc tcgaggcgca ggccgatccg gtcgatccgg cgagcgatca cgtcgggccg
    16861 gccgatcagg atcggcttga ccaggccctc gtcgaccagc acctgcaccg cgcgcaggat
    16921 ggtttcctct tcgccgtcgg cgtaggcgac ccgcattggc ttggcgcgcg cgcgctcgaa
    16981 gaccggcttc atcgcgagcc cggtgcggaa cacgaactgg gtgagcttct cggtgtaggc
    17041 gcgcatgtcg gtgagcgggc gcgtcgcgac gcccgactcc atcgccgccc tggcgaccgc
    17101 cggggcaacc tgcaccagca gccgcgggtc gaacggacgc gggatcaggt agtcgcgccc
    17161 gaagtgcggc acgcgcccgc cgtaggcccg ctgcgcgaca tcggaggcct cgcggcgcgc
    17221 gagctgcgcg atcgcgtgca cgcaggcgac ggtcatctgc tcgttgatgc cggtggcgcc
    17281 gacgtccagc gcgccgcgga agatgtaggg aaagcacagc gcgttgttga cctggttcgg
    17341 gtaatccgaa cggccggtcg cgatgacggc gtcgggtttc gcggcgcgcg cctcctccgg
    17401 ggtgatttcc ggggtcgggt tggcgagcgc gaggatcacc gggcgggcgg ccatggtcgc
    17461 caccatgtcg cgggtcagca cgccgccggc cgagacgccg aggaagacgt cggcgccctt
    17521 gacgatttcg cgcagcgtgc gggccttggt cttgcgggcg tagcgctcca ggctccggtc
    17581 gagtcccggc cggtcgtggt acagcacgcc gtccttgtcg cagacccgga tgttctcggg
    17641 cttcacgccg aggcgcacca gcatgtccag gcaggcgatg ccggccgcgc ccgcgcccga
    17701 ggccgcgact ttcacctcgc cgattttctt gccgaccacg atcaatgcgt tggtcagcgc
    17761 cgcacccacg atgatcgcgg taccgtgttg gtcgtcgtgg aagaccggga tgttcatccg
    17821 ctcgcgcaac ttgcgctcga cctggaagca ttccggcgcc ttgatgtcct cgaggttgat
    17881 gccgccgaag gtcggttcca gcgaggcgat gatctcgacc agcttgtcgg ggtcgcgctc
    17941 gtcgatctcg atgtcgaaca cgtcgatgcc ggcgaacttg cggaa
//
LOCUS       DASSNH010000065         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_700255, whole genome shotgun sequence.
ACCESSION   DASSNH010000065 DASSNH010000000
VERSION     DASSNH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700255"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1315
                     /locus_tag="VFK00_02350"
     CDS             44..1315
                     /locus_tag="VFK00_02350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7591829.1"
                     /translation="MLVSLVAFTGVLFAQTALPPKPSTPPKPVPTIPETPIPPAPQPN
                     AQAWVLMDYANGQILASSNMDQRRAPASLTKIMTEYVVNAEIAAGKIHLDDKVHISEH
                     AWRAGGAGTDGSTSFLKLDSDVPLKDLLYGMIIQSGNDAAIALAEHVAGSEQAFAEMM
                     NAYARRLGMDNTHFINASGYPASDHYSTAHDIAILSRALIKNFPEQYKISAIKSFEWN
                     GITQHNRNTLLWTDPSVDGIKTGSTKEAGFCLAASALRNGMRLIAVTMGGPDMNAATT
                     GDEALLNWGFNFYRTHDLYKAGQVLATPRLWKGEANTLQLGIAQDASVTIPRGKYDQL
                     QATLEFPQRLIAPFKQGQQVGSLKVTFDGKPVVTEPLVVLKDAPQGGFFKRLWDAILL
                     LFHRGNGTTTTPAPTVTVSSASSVAPPASAK"
     gene            1326..1613
                     /locus_tag="VFK00_02355"
     CDS             1326..1613
                     /locus_tag="VFK00_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HET7591830.1"
                     /translation="MRDLDDIEKKDGQGFQFPGSFEITAMGNANANLRERVPEILRGL
                     GLSVLEASVRERPSSQGNYVSVSVVFNCPSREKHEAAHAALRADPDIRYTL"
     gene            1724..2404
                     /gene="lipB"
                     /locus_tag="VFK00_02360"
     CDS             1724..2404
                     /gene="lipB"
                     /locus_tag="VFK00_02360"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7591831.1"
                     /translation="MAPPLPIVRPRSLLVRHLGRQPYEPVWRAMQAFTDARGPDTPDE
                     LWLVEHDPVFTLGQAGKPEHVLAPGDIPVIHVDRGGQVTYHGPGQIVAYPLLDLRRLK
                     IGVRELVLRIEQSLIDTLAEWNIVAARREGAPGVYVGDAKIAALGLRVRHGCTFHGLA
                     FNVAMDLEPFHRINPCGYAGLAVTQMLDLGGPSRLADVEEVLIAELGRQFGLIPQDAD
                     SVLPRAAA"
     gene            2582..>3552
                     /locus_tag="VFK00_02365"
     CDS             2582..>3552
                     /locus_tag="VFK00_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7591832.1"
                     /translation="MLQRAFVVLIALVFSFGAQARGAAPAAAACPASASSAVTAPLAK
                     YKPTPAPVLKPSAAQGQAAILAARLLTRFQYEALPLDDAMSQKIFDAYFKALDGSKLF
                     FTQQDINQFAAERTKLDDAIWNGDMSIPFAIFNLYEKRAGERTAYALALLKKGFNPDT
                     NDTYTFDRDKAPWAANAAALDKLWQGRVQNDWIRLKLAGKDDAEIRKTLSRRYENYLD
                     RVHQLDSEDVFQTFMNAYATAIDPHTNYFGPRASQNFDITMKLSLEGIGAVLQRDDDY
                     TAIREIVAGGPAALSGKLKVGDRIVGVGQGANGPIVDVVGWRLDDVV"
BASE COUNT          654 a         1206 c         1122 g          570 t
ORIGIN      
        1 cctccccgga attccaaacc tgatgaacaa gttgcgtccc ctgatgctgg tatccctcgt
       61 cgccttcacc ggcgtgctgt tcgcgcagac cgcgttgccg ccgaagccct cgacgccgcc
      121 caagccggtg ccgacgattc ccgaaacgcc gatcccgccg gcgccgcagc cgaacgcgca
      181 ggcgtgggtg ctgatggatt acgccaacgg ccagatcctc gcgtccagca acatggacca
      241 gcgccgcgcg ccggcctcgc tgaccaagat catgaccgag tacgtggtca acgccgagat
      301 cgcggccggc aagatccacc tggacgacaa ggtgcacatc agcgagcacg cctggcgcgc
      361 gggcggtgcc ggcaccgacg gttcaacgag tttcctgaaa ctggattcgg acgtgccgct
      421 gaaggatctg ctgtacggca tgatcatcca atccggcaac gacgccgcga tcgcgctggc
      481 cgaacacgtc gccggttccg agcaggcctt cgcggaaatg atgaacgcct acgccaggcg
      541 gctaggcatg gacaacacgc atttcatcaa cgcctcgggt tatcccgcgt ccgaccacta
      601 cagcaccgcg cacgacatcg cgatcctgtc gcgcgcattg atcaagaatt tccccgagca
      661 gtacaagatc tcggcgatca agtcgttcga gtggaacggc atcacccagc acaaccgcaa
      721 cacgctgctg tggaccgatc cctcggtgga cggcatcaag accggctcga ccaaggaagc
      781 cggcttctgc ctcgcggcct ccgcgctgcg caacggcatg cgcctgatcg cggtgacgat
      841 gggcggtccc gacatgaacg cggcgaccac cggcgacgag gcgctgctca actggggctt
      901 caacttctat cgcacgcatg atctctacaa ggccggccag gtgctggcga cgccgcggtt
      961 gtggaagggc gaagccaaca cgctgcaact cggcatcgcg caagatgcgt cggtgacgat
     1021 cccgcgcggc aagtacgacc agttgcaggc cacgctggaa ttcccgcagc gcctgatcgc
     1081 gccgttcaag cagggccagc aggtgggttc gctcaaggtg acgttcgatg gcaagccggt
     1141 ggtcaccgaa ccgttggtgg tgctgaagga cgcgccgcaa gggggcttct tcaagcggtt
     1201 gtgggacgcg atcctgctgc tgttccatcg cggcaacggc accaccacca cgccggcgcc
     1261 gaccgtgacg gtgtcgtcgg cgtccagcgt ggcgccgccc gcctcggcga agtaaggttg
     1321 cttacatgcg tgacctcgac gacatcgaga agaaggacgg ccaaggcttc cagtttcccg
     1381 gcagcttcga aatcaccgcg atgggcaacg ccaacgcgaa cctgcgcgag cgggtgccgg
     1441 aaatcctgcg cgggctcgga ctgtcggtgc tggaagccag cgtgcgcgaa cggccttcca
     1501 gccagggcaa ttacgtgtcg gtgagcgtgg tgttcaactg cccctcgcgc gagaagcacg
     1561 aagcggcgca cgccgcgctg cgcgccgatc cggatatccg gtatacgttg tgagaagcgg
     1621 ggtttggagg cgggaagcgg gggcgcactg acaaatccgt ccagcaagtt tgaaatgtcg
     1681 cggttgatcg ccatttatac gtcagttctc cgaaatacaa ccgatggcgc ccccactacc
     1741 tatcgtccgt ccccgctctt tgctggtgcg ccacctcggc cgccaaccct acgaacccgt
     1801 ctggcgcgcg atgcaggcgt tcaccgatgc gcgcgggccg gacacgccgg acgaactgtg
     1861 gctggtcgag cacgatcccg tgttcacgct gggccaggcc ggcaagccgg aacacgtact
     1921 ggcgccgggt gacatcccgg tgatccacgt cgaccgcggc gggcaggtga cctaccacgg
     1981 ccccggccag atcgtggcct atccgctgct ggacctgcgc aggctgaaga tcggcgtgcg
     2041 cgaactggtg ctaaggatcg agcaatccct catcgatacg ctggccgaat ggaacatcgt
     2101 ggccgcgcgc cgcgaaggcg cgcccggcgt gtacgtgggc gacgccaaga tcgccgcgct
     2161 gggcctgcgc gtgcgccacg gctgcacctt ccacgggctc gccttcaacg tcgcgatgga
     2221 cctggaaccc ttccaccgca tcaatccctg cggctatgcg ggcctcgcgg tgacgcagat
     2281 gctagacttg ggaggcccgt cgcggcttgc cgacgtcgag gaagtgctga ttgccgagct
     2341 tggccgacaa ttcggactca tcccgcagga cgcggactcg gtgttgcccc gtgccgctgc
     2401 gtgacttgcg gccatatccg gcgcccggat tttccgatac tttaatgctg ggccgcgccc
     2461 agacgagcct ctccgcctgc accacgcacc cgcacgcgcc agcgaacggc tggcgcattt
     2521 cgtctttgtc cgcgcttcga gcgcaaacgg ccatcccgtt gcggccgccc aaggaaagtc
     2581 catgttgcag cgtgcttttg ttgtcctgat cgccctcgtc ttttcgtttg gtgcgcaagc
     2641 ccgcggcgcc gcgccggcgg ccgccgcatg tccggcatcg gcgtccagcg cggtcaccgc
     2701 gccgctcgcc aagtacaagc ccactccagc gccggtattg aagccgagcg cggcgcaggg
     2761 ccaggccgcg atcctcgcgg cgcgtctgct gacgcgcttc cagtacgagg cgctgccgct
     2821 cgacgacgcg atgtcgcaga agatcttcga cgcctatttc aaggcgctcg acggcagcaa
     2881 gctgttcttc acgcaacagg acatcaatca gttcgcggcc gaacgcacca agctcgatga
     2941 cgcgatctgg aacggcgaca tgtcgatacc gttcgccatc ttcaacctgt acgaaaagcg
     3001 cgcgggcgaa cgcaccgcgt atgcgctggc gctgctgaag aagggtttca atcccgacac
     3061 caacgacacc tacaccttcg accgcgacaa ggcgccgtgg gccgcgaatg ccgccgcgct
     3121 cgacaagctg tggcaggggc gcgtgcagaa cgactggatc cgcctgaagc tcgcgggcaa
     3181 ggacgacgcg gagatccgca agacgctgtc gcgccgctac gagaactacc tcgaccgcgt
     3241 gcaccagctc gacagcgaag acgtgttcca gaccttcatg aacgcgtacg cgacggcgat
     3301 cgatccgcac accaattact tcggtccgcg cgccagccag aacttcgaca tcaccatgaa
     3361 gctgtcgctg gaaggcatcg gcgcggtgct gcaacgcgat gacgactaca ccgcgatccg
     3421 cgagatcgtc gccggcggcc cggccgcact gtcgggcaag ctcaaggtcg gcgaccgcat
     3481 cgtcggcgtc ggccaaggcg cgaacggccc gatcgtcgac gtggtcggct ggcgcctgga
     3541 cgacgtggtc ga
//
LOCUS       DASSNH010000066         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_491341, whole genome shotgun sequence.
ACCESSION   DASSNH010000066 DASSNH010000000
VERSION     DASSNH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491341"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFK00_02370"
     CDS             complement(<1..607)
                     /locus_tag="VFK00_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7591833.1"
                     /translation="MSVRTLFIGMDGATFTVLDDLTQANGGKPVMPFLSRIYAEGSRA
                     KLRSTPHPLTPPAWVSLMTGRGPGHHGVFDFIRAEERGDEVYFTLYDSRDCRVETIWS
                     MASRQGKKVCALNFPFTAPPPAQLNGCIVPGFIPWRHLRRNSYPDDIYERLKSIPDFN
                     PQELAWDFDREKQSGDELSDENREKWVRYHLPREQQWFKVAQ"
     gene            complement(604..906)
                     /locus_tag="VFK00_02375"
     CDS             complement(604..906)
                     /locus_tag="VFK00_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET7591834.1"
                     /translation="MSNLRGNKPIETVVAVVGDITQDWGLESDKELGAETLLARDLGF
                     TSIDLIQFCVALDQCYGRRIGFQDLLMKDGSYIGDVSLGQFADFIAARLDAQGVPT"
     gene            complement(903..>2826)
                     /locus_tag="VFK00_02380"
     CDS             complement(903..>2826)
                     /locus_tag="VFK00_02380"
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET7591835.1"
                     /translation="RNPAARPPLLANTMPMVRLGPADRERIGELASAARASDPAASAD
                     ESRGAATEVDGGEDAAAWMGLSPGAEGQAYSKHANDSATPLLDVVMELDESHIVALCR
                     VSLEHDQFIRDHVISGPVSVDPVLTGFPCVPLAVSIELMAEAAAVLAGRTDVSVIENL
                     HAFDWMSLDDDTLDIEVRAEGIDVAAGRYRASVATPRGVAVTCEVSFSRDFRLGPVAP
                     LHEPRPWNIDTSKLYTPGRYSLFHGPVFQSLRDIEVWNDSGLDVTLGDVSLAGFFESG
                     HTPCLVLNPVLLDALSHAVPCWLIQYIGPEFHLFPSSIGRIEFYESCPAGRDGILLRA
                     RQEPADPGNDDIFALRNWQVDCTDGEGRVLMRAEAISNVFFQVPPTYHALRTHPATGM
                     LGAPIHSPVPHVELWEVPLIGKQLFTQSGGICSRVLAHALLGAEEREAWRALEDAGLP
                     RRREWLSGRAAIKEAVRHWVYRRTGQLLYLADIVVSHDPQRAPSVGGWWCGSLIDAPR
                     VSLAHNADSCVAAVSAPGQPVGVDLERTDRLENPELVVDSFAVEERELVGGAGAAQQE
                     RVLRIWCAKEAAAKCLGVGFSGQPWTFRVMAADPALEHCEIAHAAGTVAVTIARKDAA
                     LIAVGTLMAQQLEAKA"
BASE COUNT          478 a          932 c          932 g          484 t
ORIGIN      
        1 attgcgccac cttgaaccac tgctgctcgc gcggcaggtg gtagcgcacc catttttcgc
       61 gattctcgtc gctcagctcg tcaccggact gcttttcgcg atcgaaatcc caggcgagtt
      121 cctgtggatt gaaatcgggg atcgatttca ggcgctcgta aatgtcgtcg ggataggaat
      181 tgcgccgcag gtgccgccac gggatgaagc cgggcacgat gcagccgttc aactgcgcgg
      241 gcggcggcgc cgtgaacggg aagttaaggg cgcacacttt cttgccctgc cggctggcca
      301 tcgaccagat ggtctccacc cggcagtcgc gcgagtcgta cagcgtgaag taaacctcgt
      361 ccccgcgttc ctccgcgcgg atgaagtcga ataccccatg gtgcccgggt ccgcgtcccg
      421 tcatcagcga aacccatgcg ggcggggtca gcggatgcgg agtcgagcgc aacttggctc
      481 ggctgccctc ggcgtagatg cgcgagagga acggcatcac cggtttgccg ccattggcct
      541 gggtgagatc gtccagcacg gtgaaggtcg cgccgtccat gccgatgaaa agggtgcgta
      601 cgctcatgtc gggactcctt gtgcgtccag gcgggcggcg atgaagtcgg cgaactgtcc
      661 cagggacacg tcgccgatat agctgccgtc cttcatcagc agatcctgga agccgatgcg
      721 ccggccatag cattgatcga gtgcgacgca aaactggatg aggtcgatgg aggtaaaccc
      781 gaggtcacgc gccagcaatg tctcggcgcc cagttccttg tcggattcca gaccccagtc
      841 ctgcgtgatg tcgcccacga cggcgacgac cgtttcgatc ggtttgttcc ctcttagatt
      901 gctcatgcct ttgcctctag ttgttgtgcc atgagagtcc ccaccgcgat gagcgcggca
      961 tccttccttg cgatcgtgac cgcgacagtg cccgccgcat gcgcgatttc gcagtgttcg
     1021 agggccggat cggcggccat gacccgaaac gtccagggct gcccggagaa gccgacaccc
     1081 aagcacttcg ccgccgcctc cttggcgcac cagatccgca agacgcgttc ctgttgcgcc
     1141 gcgccggcgc caccgaccag ttcgcgttcc tccaccgcga acgagtccac gaccagttcc
     1201 gggttctcca agcggtcggt tcgttcaagg tccaccccga ccggttggcc gggcgccgag
     1261 acggcggcca cgcaggaatc cgcgttgtgc gccagcgaaa cccggggtgc gtcgatcagc
     1321 gaaccgcacc accaaccccc gaccgacgga gcacgctgcg gatcatggct tacgacgatg
     1381 tccgccaggt acagcagttg cccggtgcgt ctgtaaaccc agtgccggac cgcctccttg
     1441 atggcggcac ggccgctcag ccattcgcgc cgccgcggca ggccggcgtc ttccagcgcg
     1501 cgccaggcct cgcgttcttc ggcgccgagc aaggcatggg ccagcacccg cgagcagatc
     1561 ccgccggact gcgtgaacag ttgctttccg atcagcggca cctcccacaa ctcgacgtgg
     1621 ggtaccggcg agtggatcgg ggcgcccagc atgccggtcg cgggatgcgt gcgcagcgcg
     1681 tgataggtcg gcggcacctg gaagaacacg ttgctgatgg cctcggcccg catcagcacg
     1741 cgaccctctc cgtcggtgca atcgacctgc cagttgcgca gcgcgaagat gtcgtcgttg
     1801 cccggatcgg cgggttcctg gcgcgcccgg agcaggatgc cgtcgcgccc cgccgggcac
     1861 gattcgtaga attcgatgcg gcctatcgac gaggggaaca aatggaactc ggggccgatg
     1921 tactggatca gccagcacgg caccgcgtgg ctcagcgcat ccagcagcac cgggttcagt
     1981 accaggcaag gcgtgtggcc ggattcgaag aaaccggcca ggctgacgtc gccgagggtt
     2041 acatcgaggc cactgtcgtt ccagacctcg atgtcgcgca ggctctggaa caccggcccg
     2101 tggaacagcg aatagcggcc tggcgtgtag agtttcgacg tgtcgatgtt ccagggccgg
     2161 ggctcgtgca ggggcgccac cggcccgagc ctgaaatctc gcgagaatga aacttcgcag
     2221 gtgaccgcga cgccgcgcgg cgtcgccacg gacgcgcggt agcgcccggc tgcgacgtcg
     2281 atcccttcgg cgcgcacttc gatgtcgagt gtgtcgtcgt cgagcgacat ccagtcgaag
     2341 gcgtgaaggt tctcgatgac gctgacgtcg gtacgccccg cgagcaccgc ggcggcttcc
     2401 gccatcagtt cgatgctgac cgccagcggc acgcagggga agcccgtgag cacaggatcc
     2461 accgacacgg gacccgaaat cacgtggtcg cggatgaact gatcgtgttc cagcgaaaca
     2521 cggcagagcg cgacgatgtg gctttcgtcg agttccatca ccacgtccag gagcggcgtg
     2581 gcgctgtcgt tggcatgctt ggagtacgct tgtccttcgg ctccgggcga aagtcccatc
     2641 caggcggccg cgtcctcgcc accgtccact tcggtggcgg cgcccctgga ttcgtcggcg
     2701 gacgcagccg gatcggatgc ccgggccgcg gaggccagct cgccgatcct ttcgcggtcg
     2761 gccggtccga gccgcaccat cggcatcgtg ttggcgagca gcggaggccg cgccgcgggg
     2821 ttgcga
//
LOCUS       DASSNH010000067         8476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_580124, whole genome shotgun sequence.
ACCESSION   DASSNH010000067 DASSNH010000000
VERSION     DASSNH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580124"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFK00_02385"
     CDS             complement(<1..1356)
                     /locus_tag="VFK00_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HET7591836.1"
                     /translation="MGASEASDPTHDSGRLLGHPRLLWVLLVLTVWFNFAFYGFRAFL
                     APYAAETFFATLPKGQALQQANLLFAGFGTLLYAVTILGGWVADNVLGEVRSLRLSLW
                     LMIPALLGMAWPGLYGFLMALAAFVLAAGLNIPLTVLVGRNYANDDPKREAGYTLYYL
                     AINLGAFIAPIICAGWIGAHYGYRWGFATAAAGMLLGTLVFEWRQRRLPGADDRPRFH
                     RRWSTPVVVVAWLALIVPTAFLMAYTTVLEIAVYALMGLLIVYFVVRCRRRGDRVQNH
                     RYIALLLLFIALVVFWALSLLSASALNFFARDHVAPLWNGQVLGLAWSFTLFQSVNPL
                     YILVLAPFLAMLWPWLDRRRINPSTPRKFGIGLLLVALGYGALWYAIARMQLPDGKVA
                     AWTLLVCYLGSTVGELALSPIGYALVGKLAAPDEASLAMGGWFFGVGISYDISGQIAT
                     LT"
     gene            complement(1440..2294)
                     /locus_tag="VFK00_02390"
     CDS             complement(1440..2294)
                     /locus_tag="VFK00_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET7591837.1"
                     /translation="MTDSNRRELEAGIQRDLSGRMTYASYLRLDGLLAAQRPVSDPAY
                     HDEMLFIVQHHVAELWLKLMIHELRGTIVDLDADQPDQALKKLARVKRIQDQLFNEWA
                     VLETMTPHEYLKFRDALGPSSGFQSLQYRTVEFLLGNKHADMLEVFSHDTEAQERLRA
                     TLEAPSLYDAALRWLARRGHAVPQAVLDRDVRQGWKHDESLLPVFRRIYEAPDKFWPE
                     YNLCETLVDVEENFQLWRFRHMKAVERIIGHRRGTGGSSGVGFLKKALDLTFFPELLE
                     VRTEIKPA"
     gene            2760..3845
                     /gene="pdhA"
                     /locus_tag="VFK00_02395"
     CDS             2760..3845
                     /gene="pdhA"
                     /locus_tag="VFK00_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET7591838.1"
                     /translation="MPIAAKFEIEYLQYLDPEGKQVRKDLPDFAQDMDQMVELYKLMV
                     STRVFDTKSIALQRTGKLGTYASCLGHEATHVGIGSALKPEDVFAPSYREYGAQLYRG
                     VRPRDVYMYWGGDERGNDYQNEPAKHDFAWSVPIGTQCLHAAGSALAFKIRREKRVAV
                     CTIGDGGSSKADFYGAINVAGARDLPLVAAIVNNQWAISVPRTIQSGAKTLAQKGIAA
                     GLYCIQVDGNDIIAVKKAMTDAVERARAGKGGSVVECVTYRLGDHTTADDARRYRGKD
                     EVEDAWTKDPVKRLRTWLEAKKAWNKKKEDKLQAECDDWMDKEVDAYLETHAQPVTAM
                     FDYTFAELPADLLKQRDEAVALDQGSH"
     gene            3847..4827
                     /locus_tag="VFK00_02400"
     CDS             3847..4827
                     /locus_tag="VFK00_02400"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7591839.1"
                     /translation="MAAITLIEGVTQALAYEMAHDDSVVVLGEDVGVNGGVFRATQGL
                     SDKFGEWRVFDTPLDETTIAGVTVGLAAQGMKPVCEAQFEGFIYPMMEQICCHAARMR
                     NRTRGRLTCPAVFRAPWGGGIHAPEHHSEANEHLFTNVPGLRVVLPSSPARAYGLLLA
                     AIRDPDPVVFFEPKRIYRQYKEEVPDDGEALPLDVCFVLRDGTDITLVTWGAQVKECL
                     EAADALAQEGVSAEVIDVATLTPLDFDTIAESVQKTGRCVIVQEAPKTAGFGAEIAAR
                     VAEECVYDLLAPVARVSGWDVHTPLYRLEMKQLPSTARVVDAAKRTLAAS"
     gene            4975..6393
                     /locus_tag="VFK00_02405"
     CDS             4975..6393
                     /locus_tag="VFK00_02405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7591840.1"
                     /translation="MADKKTFHLPDLGEGLPDATIVEWLVKEGESVKLDAPLVSMETA
                     KAVVEVPSPFTGKLLKQHGAAGDVINTGAPLAEFELDPKGKQRAENAAGGHHAPAQPT
                     KGAGAPAPANGDKVIASDEGGEIQKGGTGDRADEGTVVGAVSSSNEVRTEQAGSVGGV
                     KAVPAVRAMARKLKVDLARVQATGAGGVVTMQDVKNAAASGTAALLPSASARGAGGEG
                     SAGVVPSSALRTPSPGGRREEQRSTVSQSGKPMRTTPPSVQATGQPEQLKGVRRNMAR
                     VMADARDRVVQTTIVDDADLHHWLGKQDITGRTVRAIVAACKAEPALNAWFDDQNLTV
                     TRHPHVDLGIAVDTPDGLFVPALRNADMLDARGMREGINRLRAQVEDRSIPASELTGY
                     TISLSNFGMFAGRYGTVVVVPPCVAIVGVGKLSHDVVAVMGGIEVHRRLPISLTFDHR
                     ACTGGEAARFLKAILDDLALPH"
     gene            complement(6735..>8476)
                     /locus_tag="VFK00_02410"
     CDS             complement(6735..>8476)
                     /locus_tag="VFK00_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET7591841.1"
                     /translation="ASGGTREIGIYLDRVFATTGERVSWPAVHYQQPRYLSAFERIEV
                     PTYSQSLLAVTPYVSSRYDNVGHDGGHSEGVDVFWKPNGQFQLTATLNPDFGQVESDD
                     LVINFGATETYFSDKRPFFTENQGIFAFGLLDDNSALVYTRRVGAPADDGSGAGDIDA
                     AVKFNGSVGTTSYGVLAAGERGDAGRTFGAVRVVHDFGSRSLGMLLTRVDRPFLDRRA
                     TVLGLDQRWQPSANLTIATNVVGSDIQQSGRSSRGLGGTFIADYVMDDGWRQQWLGMY
                     FDDRLQVNDFGYLARNDLEYAHWEVRKRITDLPPDSRYRDHDWHFRIDALDNTHGLNL
                     RRQLRIERESNLRNGTDEEIELNVNSALWDDLLTRGHGALFLPPSFSLRVERHTPRHG
                     DWAHEWEIDVETGGLSGNGRLGYELEVVPTYFISDALSLSVGLDYVYTPGWLVWQHDN
                     LVGSFDRRSLELESGLDWYIDNRSQLRVKLQAIGLDAKLRGAYRVAANGRAVASDDAI
                     DSFGVRTLGLQVRYRYELAPLSYLYVVYGRGGYEQAPILDDIPGAPQFLRDSFSLRDT
                     EQWLVKLNYRFDM"
BASE COUNT         1531 a         2746 c         2888 g         1311 t
ORIGIN      
        1 ggtcagcgtc gcgatctggc ccgagatgtc gtaggagatg ccgacgccga agaaccagcc
       61 gcccatcgcg agcgatgcct cgtcgggcgc ggcgagcttg ccgaccagcg catagccgat
      121 cggtgacagc gcgagttcgc ccaccgtcga gccgaggtag cacaccagca gcgtccaggc
      181 ggcgaccttg ccgtcgggca attgcatgcg cgcgatcgca taccacaagg cgccgtagcc
      241 cagcgccacc agcagcagcc cgatcccgaa cttgcgtggc gtggatggat tgatccggcg
      301 ccggtccagc cacggccaca gcattgccag gaagggcgcg aggaccagga tgtacagcgg
      361 gttcaccgac tggaacaggg tgaagctcca cgcgagcccc aacacctgtc cgttccacag
      421 cggggccacg tggtcgcggg cgaagaagtt cagggcgctg gcggagagca ggctcagcgc
      481 ccagaacacc accagcgcga tgaagagcag cagcagcgcg atgtagcggt ggttctgcac
      541 gcggtcgcca cgacgccggc agcggaccac gaagtacacg atcagcaggc ccatgagggc
      601 gtacaccgcg atttcgagta ccgtggtata ggccatcagg aatgccgtgg gcacgatcag
      661 cgccagccac gccacgacca ccacgggagt cgaccagcgc cgatgaaagc gcggcctgtc
      721 gtcggcgccc ggcagcctgc gctggcgcca ctcgaacacc agcgttccca gcagcatgcc
      781 cgccgccgcg gtggcgaagc cccatcgata tccgtagtgg gcaccgatcc atcccgcgca
      841 gatgatgggc gcgatgaacg cgccgaggtt gatcgccagg tagtacagcg tgtagccggc
      901 ctcgcgcttc ggatcgtcgt tcgcgtagtt gcgaccgacc agcaccgtca gcgggatgtt
      961 gagtcccgcc gccaggacga aggccgccag cgccatcagg aatccgtaca ggccgggcca
     1021 cgccatcccg agcagcgccg ggatcatcag ccacagcgaa agccgcagcg agcgcacctc
     1081 ccccagcaca ttgtcggcca cccagccgcc caggatcgtc acggcataga gcagggtgcc
     1141 gaacccggca aacagcaggt tggcctgctg cagcgcctgg cccttcggca gcgtcgcgaa
     1201 gaaggtctcc gcggcatacg gggcgaggaa tgcgcggaaa ccgtagaagg cgaaattgaa
     1261 ccagaccgtg agtaccagca gcacccacag caggcgcggg tgcccgagga ggcgcccgga
     1321 atcgtgcgtc ggatccgaag cttcactggc gcccatcggc ggctcccgca gcggctggcc
     1381 ctcccggccg ctgcggacct taaacccgcg caggccgcgg cagcaacccg cggagcgcgt
     1441 caggccggtt tgatctcggt gcggacttcg agcagttccg ggaagaacgt gaggtcgagc
     1501 gccttcttga ggaacccgac gccggacgag ccgccggtgc cgcggcgatg gccgatgatc
     1561 cgttccaccg ccttcatgtg gcggaagcgc cagagttgga agttctcctc gacgtccacc
     1621 agcgtctcgc acaggttgta ctcgggccag aacttgtccg gcgcctcgta gatgcgccgg
     1681 aataccggca acagcgactc gtcgtgcttc cagccctgcc gcacgtcgcg gtccaggacc
     1741 gcctgcggaa ccgcgtgccc gcgccgggcc agccagcgca gcgccgcatc gtacaggctc
     1801 ggcgcctcca acgtcgcgcg caggcgttcc tgcgcctcgg tatcgtgcga gaacacctcc
     1861 agcatgtccg cgtgcttgtt gccgagcagg aactccaccg tgcggtactg cagcgactgg
     1921 aaaccggagg acggccccag cgcgtcgcgg aacttgagat attcgtgcgg cgtcatcgtc
     1981 tccagcaccg cccattcgtt gaacaactgg tcctggatac gcttgacgcg ggcgagcttc
     2041 ttgagcgcct ggtcgggctg gtcggcatcg agatccacga tggtcccgcg caactcgtgg
     2101 atcatcagct tcagccacaa ctccgcgacg tggtgctgca cgatgaacag catttcgtcg
     2161 tggtaggcgg ggtcggacac cgggcgctgc gccgccagca gcccatccag ccgcaggtaa
     2221 ctcgcgtacg tcatgcgccc ggagaggtcg cgctggatgc cggcttcgag ttcgcgccgg
     2281 ttgctgtcgg tcatgaaatg ggcccgttcg gagaagcttt gtggaaagtc aggccatgga
     2341 tggccgttct ggtgtgcgtt atgcgagcgc ggcgggcgat atgttcgagg agcgaagccg
     2401 cgatcaggga cgatcggcag aaactttgtg gaaagtcagg ccatgatggc cgttctggtg
     2461 tgcgtggctc agtggcgagg ttcggagaat gctcagttta gcgaagcagc gatcagcggc
     2521 gatcgcaaaa aaacgtgctt gcccgcaaaa tcctctcggc ccatgcagcg gctggcgatg
     2581 atcgaggtca agcggcggtc ggctggcttt ctgttgcgat gcagcatcgg agcgacctgc
     2641 gaatccacgc atgtgcatgc gataataggc ggcccgcttg cgccgaaacg acggctgcga
     2701 gcgctcgcgg ctccgcgcga cgtggagcca atcgaatccg aatcgaccgg gagcaacccg
     2761 tgcccatcgc cgccaagttt gaaatcgaat acctgcaata cctcgacccg gagggcaagc
     2821 aggtacgcaa ggacctgccc gacttcgcgc aggacatgga ccagatggtc gagctgtaca
     2881 agctgatggt gtccacgcgc gtgttcgaca ccaagtcgat cgcgctgcag cgcaccggca
     2941 agctcggcac ctacgcgtca tgcctgggtc acgaagcgac ccacgtcggc atcggcagcg
     3001 ccctgaaacc cgaagacgtg ttcgcgccga gttatcgcga atacggcgcg cagttgtatc
     3061 gcggcgtgcg tccgcgcgac gtgtacatgt actggggtgg cgacgagcgc ggcaacgatt
     3121 accagaacga accggcgaaa cacgacttcg cgtggtcggt gccgatcggc acgcaatgcc
     3181 tgcatgcggc cggctcggcg ctcgcgttca agatccgcag ggagaagcgc gtcgccgtgt
     3241 gcaccatcgg cgacggcggt tcttccaagg ccgacttcta cggggcgatc aacgtggcag
     3301 gcgcgcgcga cttgccgttg gtcgcggcga tcgtcaacaa ccagtgggcg atctcggtgc
     3361 cgcgcacgat ccagtccggc gccaagacgc tcgcgcagaa gggcatcgcg gcaggcctct
     3421 actgcatcca ggtggacggc aacgacatca tcgccgtaaa gaaggcgatg accgacgcgg
     3481 tcgaacgcgc gcgcgcgggc aagggcggca gcgtggtcga gtgcgtcacg tatcgcctcg
     3541 gtgaccacac cacggccgac gacgcgcgtc gctaccgcgg caaggacgag gtcgaggacg
     3601 cctggaccaa ggatccggtc aagcgcctgc gcacctggct ggaagcgaag aaggcctgga
     3661 acaagaagaa ggaagacaag ctgcaggccg aatgcgacga ctggatggac aaggaagtgg
     3721 atgcgtacct cgaaacccac gcgcagccgg tgaccgcgat gttcgattac accttcgccg
     3781 aattgccggc agacttgttg aagcagcgcg acgaggcggt tgcgctggat caggggagcc
     3841 actgacatgg cggcgattac cctgatcgaa ggcgttaccc aggcgctggc ttatgagatg
     3901 gcgcacgacg attcggtcgt ggtgctcggc gaggacgtcg gtgtcaacgg cggcgtgttc
     3961 cgcgcgacgc aaggcttgtc ggacaagttc ggcgagtggc gcgtgttcga cacgcctttg
     4021 gacgaaacca ccattgccgg cgtcacggtc ggcctcgcgg cgcaaggcat gaagcccgtg
     4081 tgcgaagcgc agttcgaggg cttcatctat ccgatgatgg agcagatctg ctgccacgcc
     4141 gcgcgcatgc gcaaccgcac ccgcgggcgc ttgacctgcc cggcggtatt ccgcgcgccg
     4201 tggggcggcg gcatccatgc gcccgaacac cattccgaag ccaacgaaca cctgttcacg
     4261 aacgtgccgg gcctgcgggt ggtgctgccg tcgtcgcccg cgcgcgccta cggcttgctg
     4321 ctggcggcga tccgcgatcc cgacccggtg gtgttcttcg aacccaaacg catctatcgc
     4381 cagtacaagg aagaggtgcc ggacgatggc gaggcgttgc cgttggacgt gtgcttcgtg
     4441 ctgcgcgatg gcaccgacat cacgctggtg acctggggtg cgcaggtgaa ggaatgcctc
     4501 gaagccgccg acgcgctcgc gcaggaaggg gtcagcgccg aggtgatcga cgtcgccacc
     4561 ctcacgccgc tcgatttcga caccatcgcc gagtcggtgc agaagacggg tcgctgcgtg
     4621 atcgtccagg aagccccgaa gaccgccggc ttcggcgccg agattgccgc gcgcgtcgcc
     4681 gaggaatgcg tctacgacct gctggcgccg gtggcgcgcg tcagcggctg ggacgtgcac
     4741 accccgctgt atcgcctcga gatgaagcag ttgcccagca ccgcgcgcgt cgtcgatgcg
     4801 gcgaagcgga cgctggcggc gagctgaggt tttcccttct cccgtcggga gaaggtggcg
     4861 cgtagcgccg gatgagggtc cggtgcaatc atgtgcccgc cgattgatac cgaaccctca
     4921 cccctgcccc tctcccggtg ggaggggggt acaacggaca aggtgatcga aatcatggcc
     4981 gacaagaaga cattccacct ccccgacctc ggcgaaggcc tgcccgacgc caccatcgtc
     5041 gaatggctgg tgaaggaagg cgaaagcgtc aagctggacg cgccgctggt gtcgatggaa
     5101 accgccaagg ccgtggtgga agtgccgtcg ccgtttaccg gcaagctgct caagcagcac
     5161 ggcgccgcgg gcgacgtcat caacaccggc gcgccgctgg ccgaattcga gctcgacccc
     5221 aagggcaagc agcgcgccga gaacgcggct ggcggccatc atgcgcctgc acaacccacg
     5281 aaaggtgccg gtgcacccgc gcccgcgaac ggggacaagg tgatcgcgtc ggacgaaggc
     5341 ggcgagatcc agaagggcgg cactggcgat cgcgcagacg aaggcacggt ggtcggcgcg
     5401 gtctcgagca gcaacgaagt gcgtacggaa caggcgggca gcgtcggcgg cgtgaaggcc
     5461 gtgccggcgg tgcgcgcgat ggcacggaaa ctgaaggtcg atctggcgcg cgtgcaggcc
     5521 accggtgcgg gtggcgtggt caccatgcag gacgtcaaga atgccgcggc ttccggcacc
     5581 gcggctctgc tcccctctgc ctccgcgaga ggggctgggg gtgagggttc ggccggagtc
     5641 gtaccctcgt ccgcccttcg gacaccttct cccggcggga ggagggaaga gcagcgcagc
     5701 acggtttcgc agtcgggcaa gccgatgcgc acgacgccgc catcggtgca ggcgaccggc
     5761 cagcccgaac aattgaaggg cgtgcgccgc aacatggcgc gggtgatggc cgatgcgcgc
     5821 gaccgcgtgg tgcagaccac catcgtcgac gacgccgacc tgcaccactg gctgggcaag
     5881 caggacatca ccgggcgcac cgtgcgcgcg atcgtcgcgg cctgcaaggc cgagccggca
     5941 ttgaatgcgt ggttcgacga ccagaacctg acggtcaccc ggcacccgca tgtcgacctc
     6001 ggcatcgcgg tcgatacgcc ggacggcctg ttcgtgccgg cgctgcgcaa cgccgacatg
     6061 ctcgacgcgc gcggcatgcg cgaaggcatc aaccgcctgc gcgcgcaggt cgaggatcgt
     6121 tcgatcccgg cctcggaact gactggctac acgatcagcc tgtccaactt cggcatgttc
     6181 gccggccgct acggcaccgt ggtggtggtg ccgccatgcg tcgcgatcgt cggcgtcggc
     6241 aagctctcgc acgacgtggt cgcggtgatg ggcggcatcg aagtgcaccg gcgcctgccg
     6301 atttcgctga ccttcgacca ccgtgcatgc accggtggcg aggctgcgcg gttcctcaaa
     6361 gcgatcctgg acgatctcgc gctgccccac taaggccggg aatcgggaat cgacagggcg
     6421 cgatgcagga aagtttcgac tttgctcgtc atcgcggaat cgcgcggcga tatccggact
     6481 gcggaggcag gacgctggag cgaacatcgg cgcagccgat ggcccgcagg gcgagccgcg
     6541 gatgcggcaa gttttccatt gtctttgcac atcggcgagt tgcgacaagg gactgcgccg
     6601 tccacggcgt tcgggtttgc aattcccgga tcaagcccgg gacaggctct gcgagcccgg
     6661 tccgccttcg cgggaatgac gtgccacggc attgatccga cgtaccctgg ggccggggtc
     6721 gctcgatccc ggccctacat gtcgaaccgg tagttcagct tgaccagcca ctgctccgta
     6781 tcgcgcaggg aaaagctgtc gcgcaggaac tgcggcgccc ccggtatgtc gtcgaggatc
     6841 ggcgcctgct cgtatccccc gcggccgtag acgacataga ggtacgacag cggcgcgagt
     6901 tcgtagcgat atcgcacctg caaaccgagg gtgcggacac cgaagctgtc gatcgcgtcg
     6961 tcgctcgcca cggcgcggcc attcgccgcc acgcggtaag caccgcggag cttggcgtcg
     7021 aggccgatcg cctgcagctt cacgcgcaac tgcgaacggt tgtcgatgta ccagtcgagc
     7081 cccgattcca gctccagtga ccgccggtcg aaacttccga ccaggttatc gtgctgccac
     7141 accagccagc ccggcgtgta gacgtagtcc aagcccaccg agaggctcag ggcgtcgctg
     7201 atgaaatagg tgggaacgac ctcgagttcg tatccgagcc ggccgttgcc gctcaatccg
     7261 ccggtctcga cgtcgatttc ccattcgtgc gcccagtcgc catggcgcgg cgtgtgccgc
     7321 tcgacgcgca gcgagaagga gggcggcagg aacagggcac catggccgcg ggtcaggagg
     7381 tcgtcccaaa gcgcgctgtt gacgttgagt tcgatttcct cgtccgtgcc gttgcgcagg
     7441 ttgctttcgc gttcgatgcg caactggcgt cgcaggttca ggccatgcgt gttgtcgagg
     7501 gcatcgatcc ggaaatgcca gtcgtggtcg cggtagcgcg aatcgggggg caggtcggtg
     7561 atgcgcttgc gcacttccca atgcgcgtac tcgaggtcgt tgcgcgccag gtagccgaag
     7621 tcgttgacct gcagccggtc gtcgaaatac atccccagcc actgctggcg ccagccgtcg
     7681 tccatcacgt aatcggcgat gaaggtgccg ccgagtcccc ggctgcttcg cccggactgc
     7741 tggatgtcgc tgccgaccac gttggtcgca atcgtcaggt tggcactggg ttgccaacgc
     7801 tggtccaggc cgagcaccgt ggcccgccga tcgaggaacg gccggtcgac ccgggtgagc
     7861 agcatgccga ggctgcgcga gccgaaatcg tgcaccacgc gcacggcgcc gaacgtgcgc
     7921 ccggcatcgc cgcgttcgcc tgcggccaag acgccgtagc tcgtggtgcc gacgctgccg
     7981 ttgaatttca ccgccgcgtc gatgtcgccg gcgccgctgc cgtcgtcggc cggcgcgccc
     8041 acgcgacgcg tgtacaccaa cgcgctgttg tcatcgagca ggccgaacgc gaagatgccc
     8101 tggttctcgg tgaagaacgg gcgcttgtcg ctgaagtagg tttcggtggc gccgaaattg
     8161 atcaccaggt cgtcgctttc cacctggccg aagtcgggat tgagcgtcgc cgtcagctgg
     8221 aactggccgt tcggcttcca gaacacgtcc acgccttcgc tgtgcccgcc gtcgtggccg
     8281 acgttgtcgt aacgcgacga gacgtagggc gtcaccgcca gcagcgactg gctgtaggtg
     8341 ggcacctcga tgcgctcgaa cgcggagagg tagcgcggct gctggtaatg caccgcgggc
     8401 cacgacacgc gctcgccggt ggtggcgaag acccggtcga ggtaaatgcc gatttcacgg
     8461 gtaccaccgc tggccg
//
LOCUS       DASSNH010000068        25741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1081476, whole genome shotgun sequence.
ACCESSION   DASSNH010000068 DASSNH010000000
VERSION     DASSNH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 25741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25741
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081476"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFK00_02415"
     rRNA            <1..249
                     /locus_tag="VFK00_02415"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            367..481
                     /gene="rrf"
                     /locus_tag="VFK00_02420"
     rRNA            367..481
                     /gene="rrf"
                     /locus_tag="VFK00_02420"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(540..827)
                     /locus_tag="VFK00_02425"
     CDS             complement(540..827)
                     /locus_tag="VFK00_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591842.1"
                     /translation="MKHSSLAFSIFGVSLVACLAFSGTASAAPDAWGPSTCTVAYDYC
                     LWVSGNTKEYCDEQLYECQRNENVASAQGVARTDDPRGPRLQTHDSLRLAI"
     gene            complement(1407..2105)
                     /locus_tag="VFK00_02430"
     CDS             complement(1407..2105)
                     /locus_tag="VFK00_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7591843.1"
                     /translation="MGKLSRYSLIGVLCSAAMLVTGICQADIIVLTFEGVGDQAAVND
                     FYNGGTDSQGNSGTDYGINFSTTSLGLIDADAGGTGNFANEPSPSTILFFLSGGAATM
                     NVAAGFDTGFSFFYTSINNPGSIVVWSGLNGTGDILASIDLSATNANCSGDPNGQFCQ
                     FDPIGVTFDGIAHSVDFGGVANQVGFDNITLGSAIPGGGGGTGVPEPGMLAMFGFGLL
                     LVGGAVWKKRRLAN"
     gene            complement(2432..3460)
                     /gene="hemH"
                     /locus_tag="VFK00_02435"
     CDS             complement(2432..3460)
                     /gene="hemH"
                     /locus_tag="VFK00_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7591844.1"
                     /translation="MPRYFGFDAAPPSPGIADTAVLLVNLGTPSAPTADAVSEYLAEF
                     LSDPRVVELPRWLWWPVLHGYVLRTRPRRSAEAYAKVWRDDGSPLLVFSEQLARAIGS
                     EISRRTGGKVVTALAMTYGAPSVRSVIADLMRGGVRRLLVLPLFPQYSGTSCGAALDA
                     VTRVMEALRWPPSIRTITDYHTDAGYLAALVGSVQAHWSRSGRGDKLLLSFHGIPEKY
                     ANAGDPYPDQCRETARRLGDALGLAEDELLVTFQSRLGRQPWLRPYTDEVVEQLARQN
                     IDTLDVLCPGFAVDCLETLEEVALRYRATFLTQGGKQFRYIPALNASKAHATALADVA
                     MPQLAGWA"
     gene            3499..3945
                     /gene="tatA"
                     /locus_tag="VFK00_02440"
     CDS             3499..3945
                     /gene="tatA"
                     /locus_tag="VFK00_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7591845.1"
                     /translation="MCHSSLSATAAQDVEARLGRLDAFVGIRSLYGRAAPAAVHWQLA
                     ESPRRAPWRPGRIPREVAMGFDSIWHWLLLLVIVLVIFGTGKLTKIGPDLGNAMRGFK
                     KAFHGDDEDEKSKQADVGEKLQADPPAAAAPHQERRDSAESKRAPE"
     gene            3963..4184
                     /gene="tatB"
                     /locus_tag="VFK00_02445"
                     /pseudo
     CDS             3963..4184
                     /gene="tatB"
                     /locus_tag="VFK00_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
     gene            4288..5085
                     /gene="tatC"
                     /locus_tag="VFK00_02450"
     CDS             4288..5085
                     /gene="tatC"
                     /locus_tag="VFK00_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7591846.1"
                     /translation="MAAPEHGPDGDPDTDSGFDLEQGIFSHLLELRSRLLKAIACVIV
                     VLACLVPFANRLYAWLAEPLVERLPLGAHLIATQVASPFITPLKLAFYTALFISMPVI
                     VYQFWAFVSPGLYRHEKRLARPLLAAAVTLFYCGCAFAYFLVLPAAFRFLTAVTPPGV
                     EMMTDITHYLDFVMLVFFAFGLCFEIPVVQVILAAVGVVDLQKLKSWRRYAIVAAAAV
                     AAVITPPDITSMVLLLIPMCLLYELGIIAVRFLVKPKVGQDAAGQAS"
     gene            5151..6272
                     /gene="dinB"
                     /locus_tag="VFK00_02455"
     CDS             5151..6272
                     /gene="dinB"
                     /locus_tag="VFK00_02455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7591847.1"
                     /translation="MHSDPGFPLDGGVSRKIVHVDMDAFYASVEQRDDPRLRGRPVVV
                     AWKGARSVVCAASYEARRFGVHSAMPALRAERLCPHAVFVPPDFVRYKAVSRQVREIF
                     ARHTDWIEPLSLDEAYLDVSDPKSGLASATAVAEAIREAIRAETRLTASAGVAPNKFL
                     AKIASDFRKPDGLFVVRPRDVLDFLAPLPVERLPGVGKVMQRKLAELGIATVTDLRAH
                     DAVALERRFGRYGRRLHELSLGIDQRPVTPDRPTLQISSEDTFEDDLALDELTPHIER
                     LAEKTWAAHRREANAPHARIARSVVLKLKTAEFRILTRTLTPPERPESARALAEIACA
                     LRARVALPARTRYRLVGVGLAGFVDRDSYEAQAALFSAL"
     gene            complement(6359..8281)
                     /gene="lpdA"
                     /locus_tag="VFK00_02460"
     CDS             complement(6359..8281)
                     /gene="lpdA"
                     /locus_tag="VFK00_02460"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7591848.1"
                     /translation="MAQTIELKVPDIGDFQDVPVIEVMVKPGDHVEKDQSLVTLESAK
                     ATMEVPASSAGTVKEVRVKVGDNVGQGSVIAVVEAEGAASPPSPIGRGAGGEETRLRD
                     AGGIRRGSERAARDGRTGAPHHGRLSDQPEPSPAPSGRAARALATAPPLPEGEGKSAA
                     PQGAAETGRKADLECRMLVLGSGPGGYSAAFRAADLGLDTILVERYGTLGGVCLNVGC
                     IPSKALLHAAEVIDSAALMGAHGIEFGKPKIDLDKLRDFKDKVVGKLTGGLTAMARQR
                     KVRAIQGVGTFVSPHELEVVNGNDRKLIRFEQAIIAAGSQAIRLSIFPWDDERVMDST
                     RALELHDVPKTLLVVGGGIIGLEMATVYRALGSAVTVVEFMDQLMPGADADLVRPLAA
                     RLKQQGVAVHLKTKVTAARAQKNGIACDFEGGSIPEKKSFDRVLVAVGRSANGNRIGA
                     DKAGVEVTDRGLIPVDTQMRTNVGHIFAIGDLVGQPMLAHKATHEAHAAAEAAAGEKR
                     FFDARVVPSVAYTDPEVAWVGVTEREAKEKSLDTGVGKFPWVASGRALGMDRAEGFTK
                     LIYDKATHRVIGGGIVGVHAGDLISEIALAIEMGAEIGDIALTIHPHPTLGESIGMAA
                     EVAEGTITDLYIPKKK"
     gene            complement(8265..8906)
                     /locus_tag="VFK00_02465"
     CDS             complement(8265..8906)
                     /locus_tag="VFK00_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET7591849.1"
                     /translation="MSKFGLFVGALGVGLGFGLQTIFNNFVSGLIILFERNLKVGDFV
                     ELASGVHGTVRDIQIRATRITTNDNIDILVPNSEFVSAQLVNWTLREASRRLHVPFKV
                     AYGTDKAMVQKAGLEAAAAVPFTQSLDGLRAPQVWLMAFDEYSMSFALVVWLTADATK
                     RVRGVLAAYNWALHDALEKYGIEIPVPQREVRVRDWTALQDQEARNQNDGPDD"
     gene            complement(9259..10695)
                     /locus_tag="VFK00_02470"
                     /pseudo
     CDS             complement(9259..10695)
                     /locus_tag="VFK00_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
     gene            10909..11793
                     /locus_tag="VFK00_02475"
     CDS             10909..11793
                     /locus_tag="VFK00_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7591850.1"
                     /translation="MLTIRNLSKTYPNGVRALRDVSLDIPNGMFGLLGPNGAGKSTLM
                     RTIATLQEPDSGTIRLGNLDVLADKPGTRQVLGYLPQEFGVYPKVSAEAMLDHFAVLK
                     GVTRRGERRDLVAALLKQVNLYDARKRKLGGFSGGMRQRFGIAQALIGDPKLVIVDEP
                     TAGLDPEERQRFLNLLAEIGERVVVILSTHIVEDVTELCPRMAIIAEGQVRLTGEPRE
                     SIRSLEGRVWRRAIDKAALPQYRERMTVLSTRLAGGATLIHVLADAMPEQGFESVPPD
                     LEDVYFGQLHHAASSKVA"
     gene            11795..15394
                     /locus_tag="VFK00_02480"
     CDS             11795..15394
                     /locus_tag="VFK00_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET7591851.1"
                     /translation="MFREIFRFELRQQLRSPLFWLVAVALAAVAFGAACSDQIQIGGG
                     VGNIHRNAPYVVVQMLGIFSVLGLFLIPIFVVGAALRDFSTGTADMMFATPMSRIAYL
                     GGRFAAGWLISIAVLVVVAVGFWLGSQMPWLDPARLGPTPWGAYGWALGVLVLPNLLF
                     LAALLFLLATVTRSMLGAYVGVLAFFMLWTIAGVTLRGGNLDHQTAGALIDPFGMGAV
                     DLVTRYWTPADRNTRIPELTGLLLGNRALWIAVGAALLAAAFWLFKPDHEGLRWFRRR
                     KRATTPDASGPAPAANIVLPVVTLRSGFAARCRQFAKLAWFDARGVLRGVPFLIMLAF
                     GLMNLGAMLALSGDMYGTSTLPVTHQMAEVMTGSYTWLLLLVVFFYAGELVWRERESR
                     VSEVVDAFPTPDWVPLLSKIAALAAVIVVFLAAGTLACMGNQLIRGYHDLQPVLYLQF
                     IGLDLLQFLLIGILAVVFQVWAGNKFVGYALIVGWIILTIVLGQFHFADHLYRYGSAP
                     STPYSDMNGFGSFWIGTLWFRAYWGAFALALLVVAALYWPRGNAAGWRARARVAQRRL
                     GVPAAVLLVCALVGFVAIGTWNFQNTHGQYRYQTYDAGQQLAARYEKDYHKYMGVPQP
                     RITDVDVNVAMHPAARRVDVDGHYVLVNKHDRPIDELLVQVPTFSTKDYSFDLDFPAH
                     TIEHADEQQGFHLYKLDTPLAPGASMDFGFKMHVAYNGFANEPAGEQVVHNGTFINSF
                     AFPHFCYDKGRELTDNNDRRKYGLGPAERLPSREDAAAHANNLISCDADWVHFQATLS
                     TSADQVALAPGYLQKTWTEGGRRYFHYVQDTPILDFFSFQSARYQVAREKWHDVDLEI
                     YYDPQHPYNIQRMFKAMKLSLDYYTTHFGPYQFRQLRILEFPDYSSFAQSFANTIPFS
                     ESIGFIADLRKPSDIDYVTYVTAHEVGHQWWAHQAIGARVKGVTMLDESLAQYSALMV
                     MKHLYGPTRMRKFLKYELDNYLRGRGGERVEEEPLALVENQPYIHYRKASVIFYALAD
                     YIGEDKVNAALRTWLDKVRFQQPPYTDTRELIADLRAQAGPGYQDLITDFFDRITLFD
                     DRMVSATAKKLAGGKYEVTMHVHAAKYYANGKGKETRAKVDIPIEIGIFAKAKDGEEE
                     DEKPLYLAKYPVKDGDSTITMTVDGVPYQAGIDPFNELVDRVSDDNRTQVTME"
     gene            complement(15545..16357)
                     /locus_tag="VFK00_02485"
     CDS             complement(15545..16357)
                     /locus_tag="VFK00_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591852.1"
                     /translation="MDEKICVFEDVYNDTSARAQAEHEKLGAFGMFARMKLWDRPKES
                     EVTLANTEKRYEPFWMAKAARRTSYIRKVVYHVKVEDAHVTTVGLFDRKFPVDARREL
                     QLQAVEECESTTSLVHYADALGHETPEKTLVDYAGRFPFQEIADSSEPRFIVPTLTAA
                     SVLQQVKSRLSAPVQADAITSDELDITSLTLFYRPVYAFQFTWKDKTGVIEIDGLSGR
                     VNKEGSMLGGAVRRLGNRDTLFDLGADFAGIVVPGGTIVVKLIDKLSKKTTA"
     gene            complement(16465..17211)
                     /locus_tag="VFK00_02490"
     CDS             complement(16465..17211)
                     /locus_tag="VFK00_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591853.1"
                     /translation="MNPTGRLPASPLVLSALALTLCVLVSACSQQSPAPPAAPSASAQ
                     AAANEAAAQNELKLYQQMLDEHSDELAAPVGQEILQKYPGTSAAAEVQKTLPQVAARA
                     KAKGEHDRLANLWYYQTADMDGLQHTASIYASGKPRSEAARLVLRRHRKWGQSVYLFA
                     PDGSRGFVCKGTCRIVMRVDGKRETWKAYLPKTGEPAMFIEDDKRFLARLEKAKVITM
                     DIESKDHGKETLKFEVGGFEPSKFKPLPKK"
     gene            complement(17282..17680)
                     /locus_tag="VFK00_02495"
     CDS             complement(17282..17680)
                     /locus_tag="VFK00_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7591854.1"
                     /translation="MKAHFPSADEYSAEKARYPGLSVVDVRAHLAAIHESYRNDVLSN
                     VNDNCLRLAVFDGEYRWHQHPDSDELFLVVEGKLLVDLAGHDTLELDPWQCAVVPAGT
                     VHRTRTSGRTVNLAFEKQQAKTVFLDARDH"
     gene            complement(17677..18018)
                     /locus_tag="VFK00_02500"
     CDS             complement(17677..18018)
                     /locus_tag="VFK00_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7591855.1"
                     /translation="MNETFAALSPDMLDIIDVDDVDPVVVGPGCLRRDLPGPEGLRVW
                     MVEMAPGSVWPTIDEHDTGETVYVAAGELIEDDRSCGAGTYLYFRPGSRHRPRTEKGA
                     RLFGINPLGRK"
     gene            complement(18127..19110)
                     /locus_tag="VFK00_02505"
                     /pseudo
     CDS             complement(18127..19110)
                     /locus_tag="VFK00_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            19420..20370
                     /locus_tag="VFK00_02510"
     CDS             19420..20370
                     /locus_tag="VFK00_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7591856.1"
                     /translation="MSRSPLNALQTFVAAARARNLTRAAERLHLTVSALSHQIRLLEE
                     RIDCTLFVRGPRGLRLTAEGQRLLDNVAPHLEAIEDALKPLCARCDNALSLSAMPSMT
                     TSWLLPRLPVFVSRHPEVELNLDSSIDLVDFADGRYDAALRYGVGDWPGLVVESLLEE
                     WLTPVASPALLAGRRRPRLEDLGDLPLLCPEDPWQEWFDLHGGKPPRRYAASFSDSES
                     RQRAAVEGLGVALGRTTMARPLIEAGQLVALFPQLMKAKRWHYLVYPERSRKHAGFMA
                     FREWLLDEAARFRAAPATGFTASLARARNPRAASRRKRAS"
     gene            complement(20390..21016)
                     /locus_tag="VFK00_02515"
     CDS             complement(20390..21016)
                     /locus_tag="VFK00_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7591857.1"
                     /translation="MDHPTPSRRIQIRHRGLPAHAACAALLLLATGAHAASIYKCVGA
                     DGRLAFRDTPCATDARQTKLNVSGLPLMDPDAPPHAVASRTPRSSSHHRTPRASARER
                     ERKQPMSWECRAADGEVFYRHTGCPGSVPGDGVVRWQDGGDSSSGKRSRHRRNAWGSV
                     SVRGVEIPRAEACRRIHSAGAAVRDGHQRDAQVSTYDRLMGRGPCAGV"
     gene            complement(21228..21716)
                     /locus_tag="VFK00_02520"
     CDS             complement(21228..21716)
                     /locus_tag="VFK00_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7591858.1"
                     /translation="MEKHGLSAALATVLLCAAGMACAQTPPVQATQTTTTTTTVSRVP
                     GALTEEQIKADIANAGYKEVSGLEFEDGVWQAKARGGNDNWVSIKVGPTTGKVYQADA
                     PSRLNEDEVKAKLTAQGYQNVHDVEFDDGLWSADATNPQGEDVDLLVDPDDGSVVASD
                     HD"
     gene            21855..23468
                     /locus_tag="VFK00_02525"
     CDS             21855..23468
                     /locus_tag="VFK00_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7591859.1"
                     /translation="MSRQATLSFHHPAAFWFGCLAVAAGVCLHLPMYVMAAPMHFHLA
                     GMPMDGEMLLGMALIPGGVLLAAYGLMPRLEQVRLTTHQGARLRFHVADGVPLNPEHW
                     RLVVVLAIALTIDVMKPASIAFVMPGMAHEYGIGMDRAGYLALSALIGTAVGSVVWGR
                     LADVFGRRAAILLSALMFMGTAICGTMPSFRWNLAMCFMMGAAAGGMLPIAFTLMAET
                     VPARHRGWLLVALGGIGTSAGYLAASGAATLLEPAFSWRALWLLNLPTGALIVLLNRY
                     IPESPRFLASAGLHEQARAVLARFAGDAGRLVRDDAGAVAPAAFLGQARAGALALLRG
                     AHGRITVALMVCGLAWGLVNFGFVLWLPANLQRLGLDARAASALMAQSALLAIPGTAL
                     VVWLYQRWSSFRSLVLFVVLTVLALLAFAAIGVAGIRSNAMTIAATVALLVAISGVIA
                     TMIPYAAEIYPVHLRGTGSGLVAASSKAGGILGALLGVAGLFDSFLWSALVIAVPMTI
                     SGVMLWRGGVETSGHELEAIQRAFECDHP"
     gene            complement(23695..25038)
                     /locus_tag="VFK00_02530"
     CDS             complement(23695..25038)
                     /locus_tag="VFK00_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7591860.1"
                     /translation="MGCLLALCAYPAFAAPPPKVPHPAPAPAASVATPAEASSIANAT
                     SDIPSLEEIQTFTRAFELIKQAYVAPVGNRQLMDSAIRGMLAGLDPHSDFLDVRDLKD
                     LTEDTSGAYSGLGIEVAQVKHQLVIVAPLDGGPAKAAGIQPGDVIEAIDGVTVQPDAI
                     DAAITKLRGPIGSKVTLSILHPDGSKPVNVPLVRQRIRIASVHARMLAPGFAYVRISE
                     FQEDTADELTDELEKLQKEHGPLRGAVLDLRSNPGGLVTSAVGVADDFLDGGLIVSTR
                     GRLSQADLKFSATPGDLLDGAPMVVLVDNGTASAAEIVTGALKDNRRALVMGRRTFGK
                     GSVQTILPLGNGDALKLTTARYYTPDGTSIQVAGIAPDIALGNVTVKADRKAEFIDDE
                     HEGDLPNHLAGTEATAANPDTGDLAIHDYALSEALHVLQGLAMARHAPAGAASTH"
     gene            complement(25138..>25741)
                     /locus_tag="VFK00_02535"
     CDS             complement(25138..>25741)
                     /locus_tag="VFK00_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7591861.1"
                     /translation="EAQALQQQRAEHATRNAALEQQRDAQKKLLAEADTRLAQQKGKL
                     ASLKHDAEALSRLLKQLQNVFADIPAQLGKNTPFAKLRGKLAWPVAGTPREGTGLLAH
                     GIVLAAKPGADVHAVAYGRVAWADFMRGYGMLVIIDHGDGWMSLYGGNESALVSAGDW
                     VRPGQAVATVGRDPEQGGAWFGLRKDGKPVDPQGWFASRR"
BASE COUNT         4117 a         8515 c         8522 g         4587 t
ORIGIN      
        1 agtacgagag gaccggagtg gacgtaccgc tggtgttccg gttgtcatgc caatggcatt
       61 gccgggtagc tacgtacgga agcgataacc gctgaaagca tctaagcggg aagcgcgcac
      121 caagatgaga tctcccgggg cacaagcccc ctgaaggacc ctcgaagacc acgaggttga
      181 taggcgcggt gtggaagtgc agcaatgcat tgagctaacg cgtactaatg atccgtgcgg
      241 cttgaccata taaccccaag acgcttcgcc gcctcgacgc atatcgaggc ccggcaaaga
      301 gcaaagacct gtcacatccc gaccccatgc tgatgtcgcg caaccgtgcg cgcatcgcac
      361 cccttccctg gcggccatag cggtgtggcc ccacccgatc ccatcccgaa ctcggaagtg
      421 aaacgcacca gcgccgatgg tagtgtggca ttcgccatgc gagagtagga cactgccagg
      481 ggcttcaaaa gcaaaaaccc ccgtcgaaag acgggggttt tcttttgcgt gcagcttctt
      541 cagatcgcaa gtcgaagaga gtcgtgcgtc tgcagccggg ggccccgcgg atcgtcggtt
      601 ctggcgactc cctgggcgga cgcaacgttc tcgttgcgtt ggcactcgta cagttgctcg
      661 tcgcagtatt ccttggtgtt cccggaaacc cacaggcagt aatcgtaggc gacggtacag
      721 gtcgagggtc cccatgcgtc cggggcggca gacgccgtgc cactgaacgc gaggcaggcg
      781 acgagcgata caccgaagat cgaaaaagcc agtgatgagt gtttcattgc aacctcctgt
      841 tggtgacgaa agccccggac aagctcatcc tgagcccgtc gggagcgccg gccgggattg
      901 ccccggaacc gggttccatg aaccggcctc gcgagaccgg tcgcacaatg cggcgcttcc
      961 ttgccccgca ggaaggcggt gggagccgat ggcattcctt caacaaagat gatgagcctg
     1021 ccgcgccggg atggaccgtt gcgtgaccac cgtcgcggtt ccgcgctctg gctggcttgg
     1081 atggtgctgc acggcagcat gcgcgccacc gcgcttcagc tccatccagc cggttgccgc
     1141 gacgcgatgg ccgccgatgc cgaggcaggc gctgtcgttg agggcgggga tgtagcaaac
     1201 cgtgcccaga atattcgacg gtctggcgcg gatgagctac ccgatcacct cgggctgggg
     1261 cgttccacag cggctgccgt ggggccgagg ctcgtctctg gtcgcgagca ttcggatcgc
     1321 aggcaaaaaa gccgtgcggc gcaagtccgc acggccttcg atgcaaggct tcaatcagaa
     1381 gcctgattat tcaacatcgt ggtgattcag ttcgccagac gccgcttttt ccagaccgct
     1441 cccccgacga gcagcaagcc gaaaccgaac atggccagca ttccgggttc gggtaccccg
     1501 gtgccgccac cgccgccggg gatggcggaa ccgagggtga tgttgtcgaa tccgacttga
     1561 ttcgccacgc caccgaagtc gactgagtgg gctatcccgt caaaagtcac gccgatcggg
     1621 tcgaattggc agaattggcc attcggatcg cccgagcaat tggcgttcgt ggcagacagg
     1681 tcaatgctgg ccaaaatgtc gcccgtgccg ttcaggccgc tccagacgac aatggaaccc
     1741 ggattgttga tggacgtgta gaagaacgag aagccggtgt cgaagccggc cgcaacgttc
     1801 atcgtcgcgg cgccgcccga gaggaagaac aggatcgtgc tcgggctcgg ctcattggcg
     1861 aaattgccgg tgccgccagc gtcggcgtcg atcaacccca aactcgtggt cgagaaattg
     1921 atgccatagt cggtgccgga atttccctgg ctatccgtcc cgccattgta gaaatcgttg
     1981 acggcggcct gatcgccgac gccctcgaag gtcagaacaa tgatgtcggc ttggcagatg
     2041 ccggtgacca gcattgccgc cgagcaaagg acgccgatta gcgagtatcg agaaagcttg
     2101 cccatggtcg ttctcctgtt cacgcgcgag ccccgtgggg tatcgtgagc aagaaaaatg
     2161 ccaagcaaag aatcgttccg aatcatatag ttatagcgtt ctgccttggc gttcagccaa
     2221 agatgtaaag cctctcgact gaaccactgc gcccggaagc caccgcctat ctgtgactta
     2281 atcacgttca atgggttaca cacgctttat gcaattcgga gggatgtaag gaaatccgtc
     2341 gcggtccgct ggcggttgtt gggacgtcca ggcaagccgg ggcatcacaa tcgggcgtcc
     2401 ggaagggcgt ctgcgctgtt gcgccgtggg atcaagccca ccccgccagt tgcggcatcg
     2461 ccacatcggc cagcgcagtt gcatgcgcct tgctcgcgtt gagcgccggg atgtagcgga
     2521 actgcttgcc gccttgggtg aggaacgtcg cgcggtacct gagtgcgacc tcctccagcg
     2581 tttcgaggca gtctacggcg aaacccgggc aaagcacgtc cagcgtatcg atgttctggc
     2641 gtgcaagttg ttccacgact tcatcggtgt agggcctcaa ccagggctgc cggcccaggc
     2701 gggactggaa ggtaaccagc agttcgtctt ctgccaggcc caaggcatcg ccgaggcggc
     2761 gcgcggtttc ccggcactgg tccgggtagg ggtcgccggc gttcgcgtat ttttccggaa
     2821 tgccgtggaa actcagcagc agcttgtcgc cacgaccgct gcggctccag tgtgcctgca
     2881 cgctcccgac cagggcagcg aggtagccgg cgtcggtgtg gtagtcggta atggtgcgaa
     2941 tcgacggtgg ccagcgcaat gcctccatca cgcgggtgac cgcgtccagc gcggcgccgc
     3001 acgaagtccc cgaatattgg gggaacagcg gcagcaccag caggcgccgc acgccgcccc
     3061 gcatcagatc cgcgatcacg ctgcggaccg atggcgcgcc ataggtcatc gcgagggccg
     3121 tgacgacctt gccgccggtc cggcgggata tttcggatcc gatggcccgg gcgagctgct
     3181 cgctgaacac cagcagcggt gagccgtcgt cccgccagac cttggcatag gcctcggcgg
     3241 aacgcctcgg acgggtgcgc agcacgtacc cgtgcagtac cggccaccac agccagcgcg
     3301 gcaattcgac cacacgcgga tcggacagga actcggcgag gtactcggac accgcgtcgg
     3361 cggtcggcgc gctcggggtt ccgaggttca ccagcagcac ggcagtgtcc gcgattccgg
     3421 gcgatggggg tgcggcatcg aaaccgaagt agcggggcat gcagcgcgat cctggtcaca
     3481 agcgcgagag cttgcgcagt gtgccacagc agcctgtccg cgaccgccgc gcaagacgtc
     3541 gaggcgcggc ttggacggtt ggacgccttc gtggggattc gttcgctgta cggccgggcc
     3601 gcgccggctg cggtacactg gcaactggcg gaaagcccgc gccgcgcccc gtggcgtccg
     3661 ggcaggattc cgcgtgaggt agctatgggt tttgacagca tctggcattg gctgctcctg
     3721 ctcgtgatcg tgctggtgat cttcggcacc gggaagctga ccaagatcgg ccccgatctc
     3781 ggcaacgcca tgcgcggttt caagaaggcc ttccacggcg acgacgaaga cgagaagtcg
     3841 aagcaggccg atgttggcga gaagctgcag gcggatccgc cggcggcagc cgccccgcat
     3901 caggaacggc gcgactccgc agagtcgaag cgcgcgcccg aatagccgga acggcggctg
     3961 cgatgatcga gctcagcttt tcgaagctgc tgctgctggc cgtgatcgcg ctggtggtgc
     4021 tcgggccgga gaaactgcca aaggccgcgc gcatggccgg cgcgatgttg aggcgtgtgc
     4081 gcctcggctg ggagagcgtg cgttcggaag tcgagcgcga gttggagatc gaagagatcc
     4141 ggcgcaacgc gcgcgaagcc gccgagcgtg ccgagtcgct ccagcatgct gcccgcgacg
     4201 ccgcttatgc ggcgcgcaca tcgatcgagc aagcggcgac cgaggcgtcg ggcccggtcg
     4261 atgccggcaa ggcgaagggt ccgtccgatg gccgcgcctg agcatggccc ggacggggac
     4321 ccggacaccg attcgggatt cgatctcgag cagggcattt tttcgcacct gctggagctg
     4381 cgttcacgcc tgctgaaggc gatcgcctgc gtgatcgtgg tgctggcgtg cctggtgcca
     4441 ttcgccaacc ggctgtatgc ctggcttgcc gagccgctgg tcgagcgcct gccgctgggg
     4501 gcgcacctga tcgcgaccca ggtcgccagc cctttcatca ccccgctcaa actcgcgttc
     4561 tacacggcgc tgttcatcag catgccggtg atcgtctacc agttctgggc attcgtgagc
     4621 ccgggcctgt accgccacga gaagcgcctc gcccgtccgc tgctggccgc cgccgtcacg
     4681 ctgttctatt gcggctgcgc cttcgcatac ttccttgtgt tgccggcggc gttccgcttc
     4741 ctcactgcgg tgacgccgcc gggtgtcgag atgatgaccg acatcacgca ctacctcgac
     4801 ttcgtgatgc tggtgttctt cgcattcggg ctgtgcttcg agatcccggt ggtccaggtg
     4861 atcctcgcgg ccgtgggtgt ggtcgacctg cagaaactga agagctggcg tcgctacgcg
     4921 atcgttgccg ccgccgcggt tgcggccgtg ataacgccgc cggacatcac gtcgatggtg
     4981 ttgctgttga tccccatgtg cctgctgtat gagctcggca tcatcgcggt gcggttcctg
     5041 gtgaagccga aggttgggca ggacgcggcg gggcaggcct cctgatgaaa aaccggggtc
     5101 agagtgcaat ttcgtgcaag catccgggaa ttgcgcagcc ctcctcgaaa ttgcactctg
     5161 accccggttt cccgcttgac ggtggtgtct cgcgcaagat cgtgcacgtg gacatggacg
     5221 cgttctacgc gtcggtcgag cagcgcgacg atccccgctt gcgcggcagg ccggtggtgg
     5281 tggcctggaa gggcgcgcgc tcggtggtgt gcgcggcgag ttacgaggcc cgcaggttcg
     5341 gcgtgcattc ggcgatgccg gcgctccgcg ccgagcggct gtgtccgcac gcggtattcg
     5401 tgccgccgga cttcgtacgc tacaaggcgg tgtcgcgcca ggtgcgcgaa atcttcgcgc
     5461 gccacaccga ctggatcgaa ccgttgtcgc tggacgaggc gtatctcgac gtcagcgacc
     5521 ccaagtccgg gctcgcctcg gccaccgcgg tcgccgaagc gattcgcgaa gcgatccgtg
     5581 cggaaacgcg gctcaccgca tcggccggcg tcgcgcccaa caagttcctc gccaagatcg
     5641 catcggattt ccggaagccg gacggcttgt tcgtggtgcg tccgcgggac gtgctcgatt
     5701 tcctggcgcc gctgccggtc gaacgcctgc ctggcgtcgg caaggtgatg cagcggaagc
     5761 ttgccgaact cggcattgcc accgtcaccg acctgcgtgc gcatgatgcg gttgcgctgg
     5821 agcgccgttt cgggcgctac ggcaggcgtc tgcacgagct atccctcggc atcgaccaac
     5881 ggccggtgac gccggatcgt ccgacgctgc agatttccag cgaagacacc ttcgaggacg
     5941 acttggcgct ggacgaactc acgccgcaca tcgagcgact ggcggaaaaa acctgggccg
     6001 cgcatcggcg tgaggcgaac gcgccgcacg cccgcatcgc ccgcagcgtg gtgctgaagc
     6061 tgaaaaccgc cgagttccgg attctgacgc ggaccctgac cccgccggag cggccggagt
     6121 ccgcgcgtgc gttggcggag atcgcgtgcg ccctgcgcgc gcgggtcgcg ctgcccgcac
     6181 gcacccgcta ccggttggtg ggcgtcggcc tcgccggctt cgtcgatcgc gacagctacg
     6241 aggcgcaggc ggcgttgttc agcgccctct aggcccagca aagtacagac gcggatcaac
     6301 gcagacaaaa agatcaatcc gcgtaaatcc gcgtcccaac gctttgcttt cgcgacgcct
     6361 atttcttctt cggaatgtac agatcggtga tggtgccttc ggcgacttcc gccgccatgc
     6421 cgatcgattc accgagcgtc gggtgcggat ggatcgtgag cgcgatgtcg ccgatttccg
     6481 cgcccatttc tatcgccagc gcgatttcgg aaatgaggtc gccggcgtgc accccgacga
     6541 tgccgccgcc gatcacgcga tgggtcgcct tgtcgtagat cagcttggtg aagccttccg
     6601 cgcgatccat gcccagcgcg cggcccgatg ccacccaggg gaacttgccg accccggtgt
     6661 cgaggctctt ttccttcgcc tcgcgttcgg tgacgccgac ccatgcgacc tcgggatcgg
     6721 tgtatgccac cgatggcacc acccgcgcat cgaagaagcg cttctcgccc gccgccgcct
     6781 ccgccgccgc atgagcctcg tgcgtggcct tgtgcgccag cattggctgg ccgaccaggt
     6841 cgccgatcgc gaagatgtgc ccgacgttgg tgcgcatctg ggtgtcgacg gggatcaggc
     6901 cacgatccgt tacttcaacg ccggccttgt cggcgccgat cctgttgccg ttggcgctgc
     6961 ggcccaccgc gaccagcacg cggtcgaacg atttcttctc cggaatgctg ccgccttcga
     7021 agtcgcaggc gatgccgttc ttctgggccc tcgcggcggt caccttggtc ttgaggtgca
     7081 ccgcgacgcc ttgttgcttc agccgtgcgg ccagcgggcg taccaggtcc gcatccgcac
     7141 cgggcatcaa ctggtccatg aattcgacca cggtgacggc gctgccgagc gcgcgataca
     7201 ccgtcgccat ttcgaggccg atgatgccgc cgccgaccac cagcaaggtt ttcggcacgt
     7261 cgtgcagttc cagggcgcgg gtggagtcca tcacccgctc gtcgtcccac gggaagatcg
     7321 acaggcgtat cgcctgcgag ccggcggcga tgatcgcctg ctcgaagcgg atcagcttcc
     7381 tgtcattgcc gttgacgact tccagctcgt gcggcgacac gaacgtgccg acgccctgga
     7441 tcgcgcggac cttccgctgc ctggccatcg cggtgagtcc gccggtgagc ttgccgacta
     7501 ccttgtcctt gaaatcgcgc agcttgtcga gatcgatctt cggcttgccg aattcgatgc
     7561 cgtgtgcgcc catcagcgcc gccgagtcga tcacctcggc agcgtgcaac aacgctttcg
     7621 aggggatgca gccgacgttg aggcacacgc cgcccagcgt gccgtagcgt tcgaccagga
     7681 tcgtgtcgag accgaggtcg gccgcgcgga acgccgcgct gtacccgccg ggaccggatc
     7741 cgagcaccag catccggcat tcgaggtccg ccttgcgacc ggtttcagcc gcgccctgcg
     7801 gagcagcgct tttcccctct ccctccggga gaggcggagc ggtagcgagc gcgcgcgcag
     7861 cgcgacccga aggggcgggt gagggttcgg gttggtcaga taaccgtcca tggtgcgggg
     7921 cgccggtgcg gccatccctg gctgcccgtt cggagccgcg cctgattccc ccggcatcac
     7981 gcaagcgcgt ctcctcaccc ccagcccctc tcccgatggg agagggcggc gaagcggcgc
     8041 cttcggcttc gaccaccgcg atcaccgacc cctgcccgac gttgtcgccg accttgaccc
     8101 tgacttcctt gacagtcccg gcgctcgagg caggcacctc catcgtggcc ttggcggatt
     8161 cgagggtgac caagctttgg tccttctcga catggtcgcc gggcttcacc atcacctcga
     8221 tcaccggcac gtcttggaaa tcgccgatgt cgggcacctt caattcaatc gtctgggcca
     8281 tcgttttggt tccttgcttc ctgatcctgt aaggcggtcc agtcacgcac gcgtacctcg
     8341 cgctgcggga ccgggatttc gatgccgtat ttctccagcg cgtcgtgcag cgcccagttg
     8401 taggccgcca gcacgccgcg cacgcgcttg gtggcatcgg cggtcagcca taccaccagc
     8461 gcgaaactca tcgagtactc atcgaaggcc atcagccaca cctgcggtgc acgcaagccg
     8521 tcgagggact gcgtgaacgg caccgcggca gccgcttcca atccggcctt ctgcaccatc
     8581 gccttgtcgg taccgtaggc gaccttgaac ggcacgtgca accgccgcga ggcttcgcgg
     8641 agcgtccagt tcaccagttg cgcgctgacg aactcggagt tcgggacgag aatgtcgatg
     8701 ttgtcgttgg tggtgatgcg ggtcgcgcgg atctggatgt cgcggacggt gccgtggacc
     8761 ccggacgcga gttcgacgaa atcacccacc ttgagattgc gttcgaacag gatgatcagt
     8821 cccgatacga aattgttgaa gatcgtctgc agcccgaatc cgaggccgac gccgagcgcg
     8881 cccacgaaca ggccgaactt gctcatcggc acgcctgcca cggcgagcgc gaccaggacg
     8941 ccgacgatca gcaacaagta attggtgatc cgcgaagcgg tgtaggcgag ggcatggtgg
     9001 gtgctctggt tgcgcgcgcc gtagcggagg aacgcccggc cgacgatacg ggtcaggacc
     9061 cataccgcca ccaggatcag gattcccgcc agcagggtgc cgacggtgac ctggatgccg
     9121 ctggcatcga tcagccggaa gccgaggatc tcgttcaatt tcgggaaggc gccggccacg
     9181 ctgtggggcg cgtcgccgcc cgaagcggat ggcgtgtcct gcagtggcgt ggcgcgcgcc
     9241 gcggccatgg atctcctcca gcagcgcgcg ccgcatgtcg gcgagcagtt gcgcgaggta
     9301 cgtggcgaat cgcgccgccg cggcaccgtc gatcacgcga tggtcgtagg acagcgacag
     9361 cggcaggacc aggcgcggcg cgaattcctt gccgttccac acgggcttca tcgacgagcg
     9421 cgagacaccg aggatcgcga cttcgggcgc gttgatgatg ggcgtgaacg cggttccgcc
     9481 gatcccgccc agcgacgaga tcgagaaaca gccgcccgac atctcgcccg gcgacagctt
     9541 gccatcgcgc gccttcttcg cgagcgccga catttcccgg gcaatctcca gcacgccctt
     9601 cttgtcgcag tcgcggatca cgggcaccac gaggccgttc ggcgtgtcgg ccgcgaaccc
     9661 gatgtggaaa tacttcttca cgaccaggtt ttcgccggaa gcgtcgagcg aggaattgaa
     9721 gtcgggatat ttccggatcg ccgcgaccga ggccttgatg aggaacgcga gcagggtgac
     9781 cttggtgcct tccttttcgt gctccttgtt gagcttgacg cgcagcgctt cgagctcggt
     9841 gatgtcggcg tcctcgaact gggtgacgtg cggaatcatc acccagttgc gcgcgaggtt
     9901 cgggccggac agcttctgga tccgcgtcag cggctgggtt tcgatctcgc cgaatttcga
     9961 gaaatcgacc tgcggccacg gcggcaggtt cagtccgcca ccaccgccca tcgcggggcg
    10021 ggcaccgccg ctcatggcgg ccttcacgaa cttctgcacg tcctcgcggc tgatgcggcc
    10081 gccgcgctcg ctgcccctga cctgcgcgag gtcgacgccc agttcgcggg cgaaggcgcg
    10141 caccaccggg ctcgcgtacg gcaccttgcc gggcattacc gatccggcgt cgaacgggat
    10201 cggtggcgtg cgcggcgcgc cggcgttttc gccgctgcgg cgcgcggcgg cgggcgtttc
    10261 cgatgccgaa gtgtcgtcgt gttccgcctg cggcgcttct tcgcgcgaat gcgcggcggg
    10321 cggcgcttcc ttccgggacg gcgctgcctg ttcctccttc ggttgcgcgg gcttttcggc
    10381 ttggccggat tccggcgagc cgcctttccc gccgctttct gccggcgccg gtcctccccc
    10441 gccgctgccg gcgtcgtccg cctcgaccgt ggcgatcacg tcgccctgcg agacttcatc
    10501 gttcaccttg accttcacgt cgcgcacggt gcccgccagc ggcgagggta cttccatcgt
    10561 ggctttcgcc gattccagcg tgaccagacc ctggtccttg tcgacgtgat cgccgggctt
    10621 gacgatgacc tcgatcaccg ggactccctg gaaatccccg atgtcgggta ccttcacctc
    10681 gcgtgtctca gccatgcccg gctccttcgg tcctcgatct gcaagacgcc cagtttcgcg
    10741 catttgcgcg gccgcgtcat gtagcggtcc gggacggcaa gccgcggcct ccgcgcgccg
    10801 aaacctgccc ttggccgaaa gtcaccgtga cctgtggccg atggacaggc atcgcgctcg
    10861 gcgggcagac tgccaggcaa ccttgaccag agcgcccgga gccgacggat gctgaccatt
    10921 cgcaacctct ccaagactta tccgaacggc gtccgcgcgc tgcgcgacgt gtcgctggac
    10981 atccccaacg gcatgttcgg gctgctgggc ccgaacggcg cgggcaagtc cacgctgatg
    11041 cggacgatcg cgacattgca ggagcccgac tcgggcacga tccgactcgg caacctggac
    11101 gtgctggcgg acaagcccgg gacgcggcag gtgctcggtt acctgccgca ggaattcggc
    11161 gtgtacccca aggtttccgc cgaggcgatg ctggaccatt ttgcggtgct gaaaggcgtg
    11221 acccggcgtg gcgagcggcg cgacctcgtc gccgcgctcc tgaagcaggt gaacctctac
    11281 gacgcccgca agcgcaagct gggcggcttt tccggcggca tgcgccagcg cttcggaatc
    11341 gcgcaggcac tgatcgggga tcccaagctc gtgatcgtgg acgagcccac ggccggactc
    11401 gaccccgagg agcgccagcg cttcctcaac ctgttggcgg aaatcggcga gcgcgtggtg
    11461 gtgatcctct cgacccacat cgtggaagac gtgaccgagc tgtgcccgcg gatggcgatc
    11521 atcgcggaag ggcaggtgcg cctgacgggc gaaccgcgcg aaagcatccg ctcgctggaa
    11581 ggccgggtgt ggcggcgcgc gatcgacaag gccgcgcttc cccagtaccg cgaacgcatg
    11641 accgtgctgt ccacgcggct cgccggtggc gcgaccctga tccacgtgct ggccgacgcg
    11701 atgcccgagc agggtttcga aagcgttccg ccggacctcg aagacgtcta tttcggccag
    11761 ttgcaccacg ccgcctcgag caaggtggcc tgacgtgttc cgcgagatct tccgcttcga
    11821 gttgcgccag caactcagga gcccgctgtt ctggctggtc gccgttgcat tggcggctgt
    11881 ggcattcgga gcggcgtgtt ccgaccagat ccagataggt ggcggcgtcg gcaacatcca
    11941 ccgcaatgcc ccctacgtcg tggtccagat gctgggcatc ttctcggtgc tcggcctgtt
    12001 cctgatcccg atcttcgtgg tcggcgcggc gctccgcgat ttcagtacgg ggaccgccga
    12061 catgatgttc gcgacgccca tgtcgcgcat cgcctacctc ggcggccgct tcgccgctgg
    12121 ctggctgatc agcattgccg tcctggtcgt ggtggcagtc gggttctggc tgggctcgca
    12181 gatgccctgg ctggatccgg cgcggctggg acccacgcct tggggcgcct acggctgggc
    12241 gctcggcgtg ctcgtattgc ccaacctgct gttcctggcc gcgctgttgt tcctgctggc
    12301 gacggtcacg agatcgatgc tgggcgccta cgtcggggtg ctcgcgttct tcatgttgtg
    12361 gaccatcgcc ggcgtcacgc tgcgcggcgg gaacctcgac caccagacgg ccggcgcgct
    12421 gatcgatccc ttcggcatgg gcgcggtgga cctggtgacg cgttactgga cgccggccga
    12481 ccgcaacacg cggattcccg aactgaccgg cctgctgctg ggcaaccgtg cattgtggat
    12541 cgcggtgggc gcggcgctgc tcgccgccgc cttctggctg ttcaagcccg accacgaggg
    12601 attgcggtgg ttccgccgcc gcaagcgcgc caccacgccc gacgcatccg gcccggcacc
    12661 ggccgcgaac atcgtgctgc cggtggtgac gctgcgcagc ggcttcgccg cgcgctgtcg
    12721 gcaattcgcg aagctcgcat ggttcgacgc ccgcggcgtg ctgcgcggcg tcccgttcct
    12781 gatcatgctg gccttcggcc tgatgaacct gggcgccatg ctcgcgctca gcggggacat
    12841 gtacggcacc agcaccctgc cggtcaccca ccagatggcc gaggtcatga ccggcagtta
    12901 cacctggctg ctgctgctcg tggtgttctt ctatgcgggc gagctggtgt ggcgcgaacg
    12961 ggaaagccgc gtcagcgaag tggtggacgc tttcccgacg cccgactggg tcccgctgct
    13021 gtccaagatc gcggcgctcg ccgcggtgat cgtggtgttc ctcgcggccg gcacgctcgc
    13081 gtgcatgggc aaccagttga tccgcggcta ccacgacctg caaccggtgc tctacctcca
    13141 gttcatcggc ctcgacctgt tgcagttcct gctgatcggt atcctcgccg tggtgttcca
    13201 ggtctgggcc ggcaacaagt tcgtcggtta cgcgctgatc gtcggctgga tcatcttgac
    13261 gatcgtcctg gggcaattcc acttcgcgga tcacctgtac cgctacggca gcgccccgag
    13321 cacgccctat tcggacatga acggcttcgg ttcgttctgg atcggaaccc tgtggttccg
    13381 cgcctattgg ggcgccttcg cactcgcgct gctggtggtg gcggcgctgt actggccgcg
    13441 cggcaatgct gccggctggc gggcgcgcgc ccgcgtcgcg cagcggcgcc tgggcgtgcc
    13501 cgcggcggtc ttgctggtct gcgccctggt cggcttcgtg gcgatcggca cgtggaactt
    13561 ccaaaacacc catgggcagt accgctacca gacctacgac gcgggccagc agctcgcagc
    13621 ccgatacgag aaggactacc acaagtacat gggcgtgccc cagccacgca tcaccgatgt
    13681 cgacgtgaat gtggcgatgc accccgcggc gcgtcgcgtc gatgtcgacg gccattacgt
    13741 gctggtgaac aagcacgacc ggcccatcga cgaactgctg gtgcaagtcc cgaccttttc
    13801 aacgaaggac tactcgttcg acctcgactt cccggcgcac acgatcgagc acgccgacga
    13861 gcagcagggg ttccatctgt acaagctgga caccccgctg gcgcccggcg cgtcgatgga
    13921 tttcgggttc aagatgcacg tcgcctacaa cggcttcgcc aacgaacccg ccggcgaaca
    13981 ggtcgtgcac aacggtacct tcatcaacag tttcgcgttc ccgcatttct gctacgacaa
    14041 gggccgcgag ctgaccgaca acaacgaccg ccgcaagtac gggctcggtc ccgccgagcg
    14101 cttgcccagc cgcgaggatg ccgcggcgca cgcgaacaac ctgatctcct gcgatgccga
    14161 ctgggtgcat ttccaggcga cgctttcgac cagcgcggac caggtcgcgc tggcgccggg
    14221 gtatctccag aaaacctgga ccgagggcgg ccggcgctat ttccattacg tgcaggacac
    14281 gccgatcctg gacttcttct cgttccagtc ggcccgctac caggtcgcgc gggaaaagtg
    14341 gcacgacgtg gacctggaga tctattacga cccgcaacat ccctacaaca tccagcggat
    14401 gttcaaggcg atgaagctgt cgttggacta ctacaccacg catttcggtc cgtaccagtt
    14461 ccgccagctg cggatcctcg agttccccga ctactccagc ttcgcgcagt cgttcgccaa
    14521 caccattccg ttctccgaat cgatcggctt catcgccgac ctgcgcaagc cttccgacat
    14581 cgactacgtc acctacgtga ccgcgcacga ggtcggccac caatggtggg cgcaccaggc
    14641 gattggcgcc cgggtcaagg gcgtcacgat gctggacgag tcgctggccc agtattcggc
    14701 gctgatggtg atgaagcacc tgtacgggcc gaccaggatg cgcaagtttt tgaagtacga
    14761 actcgacaac tacttgcgcg gccgcggggg cgagcgcgtc gaggaggagc cgctggcact
    14821 ggtcgagaac cagccctaca tccactatcg caaggcttcg gtgatcttct acgcgctggc
    14881 cgactacatc ggcgaggaca aggtcaacgc ggcattgcgc acatggctgg acaaggtccg
    14941 gttccagcaa ccgccctaca ccgacacccg cgagctgatc gcggacctgc gtgcccaggc
    15001 cggccccggg taccaggacc tcatcaccga cttcttcgac aggatcaccc tgttcgacga
    15061 ccgcatggtg tcggcgaccg cgaagaagct ggccggcggc aaatacgaag tcacgatgca
    15121 cgtgcatgcc gccaagtact acgccaacgg caagggcaag gagacccgcg cgaaggtcga
    15181 catcccgatc gagatcggca tattcgccaa ggcgaaggac ggcgaggaag aggacgagaa
    15241 gccgctgtac ctcgcgaaat atccggtcaa ggacggtgac agcaccatca ccatgacggt
    15301 ggatggcgtg ccctaccagg ccgggatcga tcccttcaac gaacttgtcg accgtgtctc
    15361 cgacgacaac cgcacgcagg tgacgatgga gtagggcgaa aagcagcgct cgcgacatgc
    15421 cacgaaggca tgtcgcgctg cttcgagcgc ccgcacaagg atgtgcgggc aggcgcccgg
    15481 caccacggat ggttgctcgt ttcgcgggat tcgaccacgg cggcggacgg tggtccgccc
    15541 cgcgtcaggc cgtggtcttc ttcgaaagct tgtcgatcag cttgacaacg atcgtcccgc
    15601 ccggcaccac gatcccggcg aaatccgcgc cgaggtcgaa tagcgtgtcg cggttcccga
    15661 gccggcggac cgcgccaccg agcatgctgc cttccttgtt cacgcggccc gacaggccgt
    15721 cgatttcaat cacgccggtc ttgtccttcc aggtgaactg gaacgcgtac accggccggt
    15781 agaacagagt cagcgaggtg atgtcgagtt catcgctggt gatcgcatcc gcctgcaccg
    15841 gcgccgacag gcgcgatttc acctgctgca ataccgaagc ggccgtcagg gtcggcacga
    15901 tgaatcgcgg ttcgctgctg tcggcgattt cctggaacgg aaagcgaccg gcgtaatcga
    15961 ccagcgtctt ttccggcgtc tcgtggccga gcgcgtcggc atagtggacg agcgaggtgg
    16021 tgctttcgca ttcctcgacc gcctgcagtt gcaactcgcg gcgggcatcg accgggaact
    16081 tgcggtcgaa cagtcccacc gtggtgacgt gcgcgtcttc gaccttgacg tggtagacaa
    16141 ccttgcggat gtacgacgtg cgccgcgcgg ccttggccat ccagaagggc tcgtagcgct
    16201 tctcggtgtt ggccagcgtg acctcggact ccttggggcg gtcccacagc ttcatgcgcg
    16261 cgaacatgcc gaaggcgccg agcttctcgt gctcggcctg cgcgcgtgcc gaggtgtcgt
    16321 tgtacacatc ctcgaagacg cagatcttct cgtccatgtc ccctccgcaa ggcgccgcgc
    16381 gcctgtggtc gtccgcgcat catgcgcgag cggtccgccg gtttcaatgg gctccgggcc
    16441 gggctggcgc gcccggcccg tggctcattt cttcggcagc ggcttgaact tcgacggctc
    16501 gaagccgccg acctcgaact tcagggtttc cttgccgtgg tccttcgact cgatgtccat
    16561 cgtgatgacc ttggccttct cgagcctcgc gaggaagcgc ttgtcgtcct cgatgaacat
    16621 cgccggctcg ccggtcttgg gcaggtaggc cttccaggtt tcgcgcttgc catcgacgcg
    16681 catcacgatc ctgcacgtgc ccttgcacac gaagcccctg ctgccgtctg gcgcgaacag
    16741 gtagacgctc tggccccatt tccggtggcg gcgaagcacc agcctcgcgg cctcgctgcg
    16801 cggcttgccc gacgcataga tgctcgcggt gtgttgcagg ccatccatgt cggcggtctg
    16861 gtagtaccac aggttcgcga gacgatcgtg ctcgcccttg gccttcgccc gcgccgcgac
    16921 ttgcgggagg gtcttttgca cttccgcggc ggcggaggtg ccggggtact tctggaggat
    16981 ctcctggccg accggcgcag ccagctcgtc gctgtgctca tccagcatct gctggtacaa
    17041 cttgagttca ttctgcgccg cggcttcgtt ggccgcggcc tgtgcggagg ccgatggtgc
    17101 ggcgggcggt gccgggcttt gctgcgagca tgccgaaacc agcacgcaca gcgtcagggc
    17161 aagagcggac agcaccagag gcgacgcggg aaggcggccg gtcggattca tgctccgcag
    17221 tatacccgtc gagtccgcat ggccggcgtc gacggaacgg cgggcgctcc agcagcatcg
    17281 gtcagtggtc gcgggcgtcc aggaacacgg ttttggcctg ctgcttctcg aacgcaagat
    17341 tgaccgtgcg accggaggta cgcgtccggt ggaccgtacc cgccggcacc accgcgcact
    17401 gccagggatc cagctccaaa gtgtcgtggc cggccaggtc gaccagcagc tttccctcca
    17461 ccaccaggaa cagttcgtcc gaatccggat gctggtgcca gcgatactcg ccgtcgaata
    17521 ccgccagtcg caggcagttg tcgttgacgt tcgacagcac gtcgtttcga tacgactcgt
    17581 ggatggcggc caggtgggcc cgcacgtcaa cgaccgacag accgggataa cgggcttttt
    17641 ctgccgaata ctcgtcggca gacggaaagt gcgctttcat tttcttccca gcggattgat
    17701 gccgaacaac cgcgccccct tttcggtgcg cggccggtgg cggctcccgg gcctgaagta
    17761 gagataggtg cccgcgccgc aactccggtc gtcctcgatg agctcgcctg cggcgacgta
    17821 aacggtttct cccgtgtcgt gttcgtcgat cgtcggccag acgctgccgg gcgccatttc
    17881 caccatccac acgcgcaggc cttcgggacc cggcaggtcg cgacgcaggc aacccggccc
    17941 gaccacgacc ggatccacgt cgtccacgtc gatgatgtcg agcatgtcgg ggctcaatgc
    18001 cgcaaaggtt tcgttcatcg caatgactcc tcaacaaccg cttcgcacat gtcgccgcgc
    18061 ggaccatgcg aagccgaccc atggccgcgc gcgcttgctg cgaccttgct gcagttgccc
    18121 caacagcatg ccgcccagga tcaggatgcc gcccaccaac atgctgatcg tcaacgcttc
    18181 gcccagcgcg agccacgccc acagcacgcc gaagatcggc accagatagg tcacagtcgt
    18241 ggcacgcggg gcgccgacgc gctggatcag gcggtaatag atcgcatagg caagtccggt
    18301 gcacaccacg cccagtgcaa cgaggctgag ccatgcctgc gctggaacgt gcgcggacgg
    18361 ccattgccac gctgcaaacg gtgcgagcaa cacggcgccc caggctagcg tggccgccgc
    18421 gaccgcaggc gccggcaagt cggagaaatg acgcttcacc aggttcgcgg agaccccgta
    18481 gaacactgct ccgagcgttc ctgccgcgac cgcggcgccc acgtccgcgc cttccacgtc
    18541 gccgctcgcc agcacggcca ccccgacgag gccgatcgcc agccccgtga ggcggcgcgt
    18601 gccgatcttt tcgccgaaca gcgcgaacgc cgcgagtgcc gcgaacggca ccgccatgct
    18661 gttgacgatt gcactgatgc cggccggtgc gcgttccgcg ctccacgcga aaagcaggaa
    18721 cggcaggagg gtattgacca ggccgatcgc ggcgatcttg aaccagccgg ttgcggcaag
    18781 ctggctgcgc gcgcgccaca gaaagggcga cagcaccagt gcgcccagcg cgaggcgcac
    18841 gtccaccagc gaaacggggc cgaacttcgg cgctgcgatc ctgaggaaca ggaacgaacc
    18901 gccccagatg gccgcgagca gcagcagctc gaccggcgtg cgccagtcgt ggcctgaatg
    18961 tccggcgacg cgcgcgccgc gcccaccacg cgggcggcgc gtcggccgcg atgattcgtc
    19021 gcggtccagc aaacgcaggg tcgcgacgtc cgtcacgtgc ccgcccatga acagcatgcc
    19081 gcccgacagc tttgccaatt ccctcaacat gtcacacctc ctaccaatac aaacgagggc
    19141 gccagtcccg gtggggactg ttttcgagat gcgcgaaggt tttttgcccg gctttgtccg
    19201 atccgaaagc cgcatcgccg ggtgcgttcc cgcacgcggt tgcctgtcca tccatgttcc
    19261 ggcgacgggg tcgggtaccg ccaaggtgct ttaacaattc cctcaacatg tgacacctcc
    19321 atccgtgttg catttaagcc ctgtggaccg caaaaataga gccatcgagg cttccgcgga
    19381 agcgacgatt tttcaagcga gacttgagga atatcgaaca tgagccgtag cccgctgaat
    19441 gccctgcaga ccttcgtcgc cgccgcgcgt gcgcgcaatc tcacgcgtgc cgccgagcgc
    19501 ctgcacctga ccgtcagcgc gttgagccac cagatccggc tgctggaaga acgcatcgac
    19561 tgcacgctgt tcgtgcgcgg gccgcgcggc ctgaggctca cggccgaagg ccagcggctg
    19621 ctcgacaacg tcgcgccgca cctcgaggcg atcgaggacg cgctgaagcc gctgtgcgcg
    19681 cgctgcgaca acgcgctgtc gctgtcggcc atgccgtcga tgaccacgag ctggctgttg
    19741 ccgcgcttgc ccgttttcgt gtccaggcat cccgaagtcg agttgaacct cgattcgtcg
    19801 atcgatcttg tggatttcgc cgacgggcgg tacgacgccg ccttgcgtta cggggttggc
    19861 gactggccgg gcctggtggt cgagtcactg ctcgaggaat ggttgacgcc ggtcgcgagt
    19921 cccgcgctcc tcgccgggcg caggcgacca cggttggagg atctcgggga tctgccgctg
    19981 ttgtgtccgg aggacccgtg gcaggaatgg ttcgatctgc acggcggcaa gccgccacgg
    20041 cgttacgccg cctccttcag cgattccgaa tcacgccagc gcgccgccgt cgaggggctg
    20101 ggcgtcgcgc tggggcgcac cacgatggcc cggccgctga tcgaagcggg gcaattggtg
    20161 gcgctgttcc cacagttgat gaaggcgaag cggtggcact acctcgtgta cccggagcgc
    20221 tcacgcaagc acgccggatt catggccttc cgggagtggc tgctggatga agccgctcgc
    20281 ttccgggccg cgcccgcgac cgggttcacg gcgtcgctcg cccgtgcacg caacccgcgt
    20341 gcggcgagcc gacgcaagcg cgcttcgtaa aacacggcgc ctcaacccgt cacacgcccg
    20401 cgcaggggcc gcgccccatc agtcgatcgt aggtggagac ctgcgcatcg cgctggtggc
    20461 cgtcgcgcac cgcggcgcca gcggagtgga tgcgccggca agcctcggcg cgcgggatct
    20521 ccacgccacg caccgacacg gagccccagg cgttgcggcg gtgacgcgac cgcttgccgg
    20581 aggatgaatc gccgccgtcc tgccatcgca ccacgccgtc gcccggcacc gagcccgggc
    20641 agccggtgtg ccggtagaaa acttcgccgt cggcggcgcg gcattcccac gacatcggct
    20701 gtttgcgttc gcgttccctg gccgatgcgc ggggggtgcg gtgatgggag gaggatcgcg
    20761 gcgtgcgcga cgccacggcg tgtggcggtg cgtccggatc catcagtggc agcccggaca
    20821 cgttcaactt cgtttgccgc gcgtccgtcg cgcagggcgt gtcgcgaaac gcgagccggc
    20881 catccgcgcc aacgcatttg tagatcgaag ccgcgtgcgc gccggtggcg agcaggagca
    20941 acgccgcaca agctgcatgc gcgggcaacc cgcgatgccg gatctggatg cggcgagatg
    21001 gtgttggatg atccatggcg gcttccctca ctgtcgtgaa ggaatggtcg gctcgagccg
    21061 accgccgcgc cagcgggcac cgccgcccga acgtgtaggg aaatgccgat ggccatgcat
    21121 ggttgcgtgc gggcgaccag gtagaccacc accgaggccg gcgcacgacg aaaaaagccg
    21181 gcgcggtacc acgccggctt taggagcctt gatccgtgcc aggcgcgtca atcgtgatcc
    21241 gaggcgacca cgctgccgtc gtcgggatcg accaggagat ccacgtcttc gccttgcgga
    21301 ttggtggcgt cggcgctcca gaggccgtcg tcgaactcga cgtcatggac gttctggtag
    21361 ccctgtgccg tcagcttggc cttcacttca tcctcgttga gacgcgacgg cgcatcggcc
    21421 tggtagacct tgccggtggt cgggcccacc ttgatgctca cccagttgtc gttaccgccg
    21481 cgcgccttgg cctgccaaac gccgtcttcg aactccaggc ccgaaacttc cttgtacccg
    21541 gcattggcaa tatcggcctt gatctgctcc tcggtgagcg cgccgggcac cctcgaaacc
    21601 gtggtcgtcg tggtggtggt ctgggtggcc tgcaccggcg gggtctgcgc gcaggccatg
    21661 cccgccgcgc acaacaggac cgttgccagt gccgccgata atccgtgctt ctccatgatg
    21721 gtattcctcc gtttggaagt gcgattcagc ttggatggag ccggatcaac ggccggtgaa
    21781 tcccgccgcc acccgtcagc gatcgttcat ttgccgtcgc ctgctacgct gcgccaattt
    21841 cggaagcctt ttgaatgagc cggcaggcga ccctttcgtt ccaccacccc gccgccttct
    21901 ggttcggctg tcttgcggtc gcggctggcg tgtgcctgca cctgccgatg tacgtgatgg
    21961 ccgcaccgat gcacttccac ctggccggca tgccgatgga tggggagatg ctgctgggca
    22021 tggccctgat cccgggcggc gtgctgctcg cggcgtacgg tttgatgcca cgcctcgaac
    22081 aggtgcggct gacgacccac caaggcgcac ggctgcgttt ccacgtcgcc gacggcgtgc
    22141 cgctcaaccc cgagcactgg cggctggtgg tggtgctggc catcgcattg acgattgacg
    22201 tgatgaagcc cgcgtcgatc gcattcgtga tgccggggat ggcgcacgag tacggcatcg
    22261 gcatggatcg cgcggggtat cttgcgttat cggcgctgat cggcaccgcc gtcggttcgg
    22321 tggtgtgggg ccggctcgcc gacgtgttcg ggcggcgcgc ggcgatcctg ctctcggcgc
    22381 tgatgttcat gggcaccgcg atctgcggca cgatgccttc gtttcgctgg aacctcgcga
    22441 tgtgcttcat gatgggggcg gcggccggcg gcatgctgcc gatcgctttc acgctgatgg
    22501 ccgaaaccgt gcctgcccgg caccgcggct ggctgttggt cgcactcggc ggcatcggaa
    22561 cctcggcggg ttatcttgct gcgtcgggcg cggccacctt gttggagccc gcattcagtt
    22621 ggcgtgcgct gtggctgttg aacctgccga ccggcgcgct gatcgtgctg ctgaaccgtt
    22681 acatcccgga gtcgccgcgg ttcctcgcga gcgcgggact gcacgagcag gcgcgcgcgg
    22741 ttctcgcgcg ctttgcgggg gatgcaggca ggctggtgcg agacgatgcc ggggcggtcg
    22801 cacccgcggc attccttggc caggcgaggg ctggcgcgct cgcactgtta cggggcgccc
    22861 acggacgcat cacggtggcg ctgatggtct gcggactcgc gtggggactg gtcaacttcg
    22921 gtttcgtgct gtggctgccc gcgaacctgc aacggctggg gctggatgcc cgtgccgcga
    22981 gcgcgttgat ggcgcaatcc gccttgctcg cgatccccgg caccgcgctg gtggtctggc
    23041 tctaccagcg ctggagcagc ttccggtcgc tggtgctgtt cgtggtgctg acggtgctgg
    23101 cgctgctggc gttcgcggcg atcggggtcg ccggcatccg cagcaatgcg atgacgattg
    23161 ccgcgacggt ggcgctgctg gtcgcgatca gcggcgtgat cgcgaccatg attccctatg
    23221 cggccgagat ctacccggtg cacctgcgcg ggaccggctc gggactcgtt gccgcgagtt
    23281 ccaaggccgg cggcatcctg ggcgcgctgc tgggcgttgc cggcctcttc gacagcttcc
    23341 tgtggtcggc gctggtgatc gcggtgccca tgacgatctc cggcgtgatg ctctggcgcg
    23401 gcggtgtcga aaccagcggc cacgaactgg aagcgatcca gcgggcattt gaatgcgatc
    23461 atccctgatc gctgcatcgt tgcaggtcgg accaatcgca accattgcgg cgtgatggag
    23521 accagaccga tcttccattt ggccggacga tccacagcag ccgctcagcg tggaattccg
    23581 tccaaaaagg tgtccggcgg gtagggcgga acccgcgaag cggattccgc cgaatcggtt
    23641 cacggctcgc gcggcggacg gtgccgtcct gcagcgccgg aatgggcggc gcagtcaatg
    23701 tgtgctggct gcccccgccg gcgcgtgccg cgccatcgcg agtccctgga gcacgtgcaa
    23761 cgcctccgac agcgcgtagt cgtggatcgc gagatcgccc gtatccgggt tcgcggccgt
    23821 ggcctcggtg cctgccagat ggttgggcag gtcgccctcg tgctcgtcgt cgatgaactc
    23881 cgccttgcga tcggccttga cggtgacatt gccgagcgcg atgtccgggg cgatgccggc
    23941 gacctggatc gacgttccgt cgggcgtgta gtagcgcgcg gtggtgagct tcagggcgtc
    24001 gccgttgccg agcggcagga tcgtctgcac cgagcccttg ccgaaggttc gccgccccat
    24061 caccagcgcg cgacggttgt ccttcaaggc gccggtgacg atttccgccg cggacgcggt
    24121 gccgttgtcg accagcacca ccatcggcgc gccgtccagg agatcgcccg gggttgcgct
    24181 gaacttgagg tccgcctgcg agagccgccc gcgggtgctg acgatcaggc cgccgtcgag
    24241 gaaatcgtcg gcgacgccga ctgcgctggt cacgaggccg ccgggattgg aacgcaggtc
    24301 gagtaccgcg ccgcgcagcg gtccatgttc cttctgcagc ttttcgagct cgtcggtgag
    24361 ttcgtcggcg gtgtcttcct ggaattcgct gatgcgcacg taggcgaatc cgggcgccag
    24421 catccgcgca tgcacgctgg cgatccggat gcgttggcga accagcggga cgttcaccgg
    24481 cttgctgccg tcggggtgca ggatcgacag cgtgaccttc gagccgatcg gcccgcgcag
    24541 cttggtgatg gccgcgtcga tggcatcggg ttgcaccgtc acgccatcga tcgcctcgat
    24601 aacatcgccg ggctggatgc cggcagcctt ggccgggccg ccatccagcg gcgccacgat
    24661 caccaactgg tgcttgacct gcgcgacctc gatgccgagt cccgaatatg cgcccgaggt
    24721 gtcctcggtc aggtccttga gatcccgcac gtcgaggaaa tcgctgtgcg ggtcgaggcc
    24781 cgccagcatt ccccggatcg ccgaatccat caattgccgg ttgccgaccg gtgcgacata
    24841 agcctgcttg atcaactcga aggcgcgggt gaaggtctgg atttcctcca ggctcgggat
    24901 gtcgctggtt gcgttggcga tcgaggaagc ctcggccggc gtggctacgc tggcggcagg
    24961 cgcgggtgca ggatgcggca ccttgggcgg tggcgcggcg aaggcggggt atgcgcacag
    25021 cgccagcagg caccccaacc gaatttgcca gtttcgaatc attgcgcacc gagggtcgac
    25081 aggggatcag ggaccgcgcg gacgggcggt ggttcattgt aggcgcttcg gcgccggtca
    25141 gcgccgggac gcgaaccagc cctgcggatc gaccggcttc ccgtccttgc gcagcccgaa
    25201 ccacgcgccg ccctgttccg ggtcgcggcc gacggtcgcg accgcttggc ccggccgcac
    25261 ccagtcgccg gcggaaacca gtgccgactc gttgccgccg tacaggctca tccagccgtc
    25321 accatggtcg atgatcacca gcatcccgta gccgcgcatg aagtcggccc aggccacccg
    25381 gccataggcg actgcgtgca catccgcgcc gggcttggcg gcgagcacga tgccgtgggc
    25441 cagcagcccg gtgccctcgc gcggcgtgcc cgccaccggc catgcgagct tgccccgaag
    25501 tttcgcgaac ggggtgttct tgccgagctg cgccgggatg tcggcgaaga cgttctggag
    25561 ttgcttgagc aagcgggaga gcgcctccgc atcgtgcttc agggaggcga gcttgccctt
    25621 ctgctgcgcc aatcgtgtgt ccgcttcggc gagcagcttc ttttgcgcgt cccgttgctg
    25681 ttcgagcgcg gcattcctgg tggcgtgctc ggcgcgctgc tgttgcaggg cctgggcttc
    25741 c
//
LOCUS       DASSNH010000069        12697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1369983, whole genome shotgun sequence.
ACCESSION   DASSNH010000069 DASSNH010000000
VERSION     DASSNH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369983"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VFK00_02540"
     CDS             <1..1199
                     /locus_tag="VFK00_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7591862.1"
                     /translation="AGLRKAGRNVIDIGMAPTPLVYFGSFHLRTGCCVAVTGSHNPPD
                     YNGFKIVVGGETLSGKAITGLHDRIAEDRLFVAPTPGTLSERDISQDYVDRIAGDVQA
                     DRRLKVVVDAGNGVAGGLGPRVLEAIGVDVEELYCEIDGTFPNHHPDPSEPHNLQDLV
                     RMVQRLGADIGLAFDGDGDRLGVVTRDGEIVFPDRVLMLFAQDVLVRNPGATIMYDVK
                     CTGHLQPLILRAGGSPLMWKTGHSLIKSKMRETGAQLAGEMSGHFFFRERWYGFDDGV
                     YAAARLLEILADDPEGRTPEEIFATLPKGVSTPELHVQMQEGQNHPFIARFREAAHFE
                     GARLTTIDGLRADWPDGWGLVRASNTTPVLVLRFDADNPDALKRIQEAFRAQILACDS
                     TLTLPF"
     gene            complement(1370..2101)
                     /locus_tag="VFK00_02545"
     CDS             complement(1370..2101)
                     /locus_tag="VFK00_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591863.1"
                     /translation="MRSVHFRSFAFAIFAALSLALAACVSGVNGSVDVAAGSSVSDAT
                     TVNGSVNVGARAKAHKASTVNGSVHLADGAQVGEAETVNGGITLGENATAGSVTTVNG
                     KVSMEKGATVSGDVEAVNGTLTLAAGSEVKGSLTNVNSQISVDGAHVGQGITTVNGDI
                     EVTGNAVVDGGIRVKKPNENGVFGIHFDSDNVPRIVIGPGATVNGPLTFERPVHLYIS
                     DQAHVAGPITGAEATKYSGATPPSS"
     gene            2341..3090
                     /gene="gpmA"
                     /locus_tag="VFK00_02550"
     CDS             2341..3090
                     /gene="gpmA"
                     /locus_tag="VFK00_02550"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET7591864.1"
                     /translation="MHTLVMIRHGQSLWNQENRFTGWVDVDLSEQGWREAREAGELLK
                     REGYGFDIAHTSVLTRAVHTLWGVQDAMDLAWLPVVTDWRLNERHYGALAGLNKAEVA
                     ARHGDEQVHVWRRSYDVPPPPLDPSENTFAHDPRYAKLDPAQIPLTECLKDTVARVLP
                     YWHEVLAPAIRSGQRVLVSAHGNSMRALVKYLDGISDADIPGLNIPNGVPLVYRFDNA
                     LTPVEHFYLGDADAIAAKAAAIANQGRANAG"
     gene            complement(3099..4862)
                     /locus_tag="VFK00_02555"
     CDS             complement(3099..4862)
                     /locus_tag="VFK00_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7591865.1"
                     /translation="MTPLRLLPRVVHVAAVLVRYRVDDLIDEQHALRPLRWLRVFLPR
                     PRKPIAALPRGARLRAALTELGPIWVKAGQVLSTRRDLIPEDVAEELAHLQDRVTPFP
                     GAEARAIVEESLGKPIGELYASFDETPLASASVAQVHAARLHPRAPTSRPPPGASRHH
                     PLPQAGEGNAERPTAAAFVGDREVIVKVLRPNIRKNIRQNLELLDALAWLVQRWHPQA
                     DKIRPRDMFNEVARTLHNELNLQNEGANASLLRRNFEGSDDLYVPEVFWSHTCEKVLT
                     MERVYGIPADDVAALDKAGIDRRVLAEKGIRLFYEQVFRDNFFHADAHPGNIWVDPAR
                     SREPRFIALDFGIMGSLPEEDQYWLAENFIAMFERDYHRIAELHIAAGWMPRSIRVDE
                     LEAAIRTVCEPYFTRPLSEISLAEVVAKLFSVARKFELTLQPQLILLQKTLLNVEGLG
                     RQVDPGIDIWSVAQPVLERILADRYSARHALREMRKRIPEWLRIAPLIPEKIHDALEA
                     LARGNGPLQFGERELEHLRMLQREVQRRILAAAFGGALLVGAGLVFALGDRYGIWPPI
                     VLAVIGVASFVFAWPWSRAKE"
     gene            complement(4859..5515)
                     /locus_tag="VFK00_02560"
     CDS             complement(4859..5515)
                     /locus_tag="VFK00_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET7591866.1"
                     /translation="MPEREPNAWIPHPLRKLGGLALTLALNRLVALDPETRQAIGRLE
                     GRRIGVYLRGPELAFALAARNSTLQIEPPPEAGLDGSASDFEVKATPGSLLAMVLGRD
                     GDARPPGKVEIAGDAELARQVQKLAREYRPDFEAAFTAVFGEVVGTTIARALRDATKW
                     AGEGARHFRDDTIDWLRDEARVTVPHPEMNAFLDDVDDLRERTERLAARMARLRNDKP
                     "
     regulatory      5674..5823
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            5854..6411
                     /locus_tag="VFK00_02565"
     CDS             5854..6411
                     /locus_tag="VFK00_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET7591867.1"
                     /translation="MEAFLVSLGAVALAEIGDKTQLLSLVLAAKFRKPWPICGGILVA
                     SLLSHAIAAELGAWLAHWMTPEVQRWLIGLSFLAVAAWALLPEKEEPADAPPRRGHGV
                     FVVSVIAFFVAELGDRTQVATVVLGAHYQPLWQVIAGSTAGMLVANVPVVFLGARFAH
                     KLPLRAARLAAAALFAALGVWILLR"
     gene            complement(6415..6879)
                     /locus_tag="VFK00_02570"
     CDS             complement(6415..6879)
                     /locus_tag="VFK00_02570"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET7591868.1"
                     /translation="MLHVILYQPQIPPNTGNAIRLCANAGTSLHLIRPLGFELDHAKL
                     RRAGLDYHEFVNVMAHDDFESCLEALQQPRVFAFTSRARHRYTDTRFADGDALLFGRE
                     TNGLPEAVLQTIPEPRRLRLPMQPDNRSINLSNAVAIAVYEAWRQLGFPGGG"
     gene            complement(7209..7907)
                     /locus_tag="VFK00_02575"
     CDS             complement(7209..7907)
                     /locus_tag="VFK00_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7591869.1"
                     /translation="MQRCLVTGANRGLGLAFVSELLERGARVMACCRDPAHAQVLDAL
                     RAAHADELVIHRLDVADAQAIEALPAVAARHLQRIDLLVNNAGVLVSGERFGNVKAES
                     LAESFAVNASAPLLITQALAPLLALGNKPRVLCVTSQIASIAQSARFRTLSYAMSKAA
                     LNMAVKRLAAELTPRGIVVLAVHPGWLKTEMGGKGATLSPADSAHALLGLVERAGNEE
                     AGTFLAYDGAALPW"
     gene            8134..10980
                     /locus_tag="VFK00_02580"
     CDS             8134..10980
                     /locus_tag="VFK00_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7591870.1"
                     /translation="MRSHWLAWFAVLGFSLIAGAAQAAAAAPKLDIDIPYTRFTLPNG
                     LTVVVSEDHKAPIVAVSVWYHVGSGYEPEGKTGFAHLFEHLMFQGSEHHKGEYFQPFE
                     LVGATGMNGTTWLDRTNYFETVPTTALDMALWMESDRMGHLLGAIGQPELDEQRGVVQ
                     NEKRQGENQPYGRAFEDIQANAFPANHPYHHDTIGSMADLNAASLDTVKQWFKDYYGA
                     ANTTLVLVGDITPAQAREKAMEYFGDIPAGPRVPRPQAWVTARDTSTSGTMTDNVAQV
                     RIYREWNTPGLDAEGNDRLMLDLAAQVLGGSKTSRLYQRLVYKDKLADDVSVGNMTFE
                     LANMTLLQVDVKKGVDPKKVEAAIADEWSKFLKDGPTADELERVKATYQASTIRGLER
                     IGGFSGKAVTLAEGQVYRGDPGAWKKDYEVTMAATAAEVKAAADKWLSKGDYTLTVVP
                     GKVEAEDAATIAGRAAIPGRPSDQLTPKADYKVTEGDIDRSKGVPTVTAFPDLTFPEL
                     QHGKLKNGIEVVLAERHSIPVVQVEALFDAGYAADQGRKLGTSSFTMGMLDEGTRDLD
                     SVEIAKRKQRLGAEIASGCDLDICNAWMSALDANLADSLALYADIVRNPAFRDADVAR
                     LRGQWLAGIAQEKTQPTALAQRLLPPILYGKGNAYAIPFTGSGTEASIKSLTTDDMRG
                     FVHDYIRPDNMKILVAGDTTLDAIIPQLDKVFGDWKAPASPVPAKNLAKVEYPAKSSV
                     YLVDRPGAQQSVIIAGLVAPSTKAPNNLEIGTMNGAFGGTFTSRLNMNLREGKHWAYG
                     AFSFSPSAIGQRPFLMYAPVQTDKTAPSAEEVLKEARAVVGDKPLTDKEIQKIKDNDI
                     RKMPGQYETAQAVLNAMEGIVKYDRPDDYVQTLKGRIEAQQDGEVNAAAKEVIHPARL
                     TWVIVGDLSKIEKPVRALDLGEVHVIDADGNPVK"
     gene            11201..11962
                     /gene="ubiE"
                     /locus_tag="VFK00_02585"
     CDS             11201..11962
                     /gene="ubiE"
                     /locus_tag="VFK00_02585"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET7591871.1"
                     /translation="MRETRIPGATTHFGYREVPVAEKQRLVGEVFSSVAGKYDVMNDL
                     MSFGVHRLWKRFFVATSGVRRGDRVLDLAGGTGDIAALLLPRVGKQGDVVVADINAAM
                     LRAGRDRLLDRGLLRGLRFAQVNAEALPFPDASFDAVTIAFGLRNVTDKDKALAEMHR
                     VLKPGGRVLILEFSKVNQDWLKPLYDFHSFQVLPRIGKLVAHDRDSYRYLAESIRKHP
                     DQATLKAMLEAAGFRNVEVRNLSAGIVAIHRGYRI"
     gene            11972..12241
                     /locus_tag="VFK00_02590"
     CDS             11972..12241
                     /locus_tag="VFK00_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591872.1"
                     /translation="MQSEPRSPLELNAAMRIVSAIAAASVLVGLVVAPYTDRLRMASN
                     AVFCVVLACWAIHGDRTRRNRMLTALLLLIFLAIFALRVREHGFP"
     gene            12268..>12697
                     /locus_tag="VFK00_02595"
     CDS             12268..>12697
                     /locus_tag="VFK00_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7591873.1"
                     /translation="MRELYPEIEPYRTGRFPVSDRHTLYLEECGNPDGLPVVFLHGGP
                     GAGLARYHRRFFDPARWRVVLFDQRGAGRSTPFADLEDNTTQELVDDIEKIRAHLGIV
                     RWMVFGGSWGSTLALAYAEAHPERVFALVLRGIFLGRPEEI"
BASE COUNT         2117 a         4230 c         4236 g         2114 t
ORIGIN      
        1 tcgccggcct gcgcaaggcc gggcgcaacg tcatcgacat cggcatggcg ccgacgccgc
       61 tggtgtattt cggcagcttc cacctgcgca ccggttgctg cgtcgcggtc accggcagtc
      121 acaacccgcc cgactacaac ggcttcaaga tcgtggtcgg cggcgaaacg ctctcgggca
      181 aggcgatcac cggcctccac gaccgtatcg ccgaggatcg cctgttcgtc gcacccacgc
      241 cgggcacatt gtccgaacgc gacatcagcc aggactacgt cgatcgcatc gccggcgacg
      301 tgcaagccga tcgacgcctg aaagtcgtgg tcgatgccgg caacggcgtc gccggcgggc
      361 tcggcccgcg cgtgctcgaa gccatcggcg tcgatgtcga ggagctgtac tgcgagatcg
      421 acggcacctt ccccaaccac catccggatc cgagcgaacc gcacaacctg caggacctgg
      481 tgcgcatggt gcagcgcctg ggcgcggaca tcggcctcgc cttcgacggc gacggcgacc
      541 gcctcggcgt cgtgaccagg gacggcgaga tcgtctttcc cgatcgcgta ttgatgctgt
      601 tcgcgcagga cgtgctggtg cgcaatcccg gcgccacgat catgtacgac gtgaagtgca
      661 ccggccacct gcagccgctg atcctgcggg ccggcggcag tccgctgatg tggaagaccg
      721 gccactcgct gatcaagtcc aagatgcggg aaaccggcgc gcagctcgcc ggcgagatga
      781 gcgggcactt cttcttccgc gagcgctggt acggcttcga cgacggcgtc tacgccgctg
      841 cgcgcctgct ggaaatcctc gccgacgatc cggaaggccg tacgccggag gagatcttcg
      901 cgaccctgcc caagggggtg tccacgcccg agctgcacgt ccagatgcag gaaggccaga
      961 accatccctt catcgcgcgc ttccgtgaag ccgcgcattt cgagggcgca cggctcacga
     1021 cgatcgacgg gctgcgtgcc gactggccgg acggctgggg cctggtgcgc gcgtccaaca
     1081 cgacgcccgt gctggtgctc cgcttcgacg ccgacaatcc ggatgcgctg aagcgcatcc
     1141 aggaagcgtt ccgcgcgcaa atccttgcgt gcgacagcac gctcacgctg ccgttctgac
     1201 ttcgacgttt ttgctcttgc ccgccatccc ggcccaggcc gggatccatg ctccttcgcc
     1261 aacccgggga cgtggacatt tgcgcgagcg ccggcccgaa gaagattcct tcagcgccgc
     1321 aattcgctag acacgcttgc aagcggcgcg gtacgagaca agccctgcgt cacgacgacg
     1381 gcggcgttgc acccgagtac ttcgtagcct ccgcaccggt gatcgggccg gcgacgtgcg
     1441 cctggtcgct gatgtagaga tggaccggtc gttcgaaggt gagcggtcca ttcacggtgg
     1501 cgccggggcc gatcacgatc ctcggcacgt tgtcgctgtc gaagtggatg ccgaacacgc
     1561 cgttttcgtt cggcttcttg acccggatgc caccatcgac cacggcgttg ccggtgacct
     1621 cgatgtcgcc gttgacggtc gtgatgccct ggccgacgtg ggcgccgtcc acggagattt
     1681 ggctgttgac gttggtcagc gatcccttca cctcggatcc ggcggcgagg gtgagcgtgc
     1741 cattgaccgc ctcgacgtct cccgagaccg tggcgccctt ttccatgctc accttgccat
     1801 tgacggtggt gacgctgcct gcggtggcgt tctcaccgag cgtgatgccg ccgttgaccg
     1861 tttcggcctc gccgacctgg gcgccatccg ccaggtggac cgaaccattg accgtggatg
     1921 ccttgtgggc cttggccctg gcgccgacgt tcaccgaccc gttcacggtg gtcgcgtccg
     1981 agacgctgga gcccgcggcg acatcgaccg agccgttcac gccgctcacg caggcggcga
     2041 gcgccagcga cagggctgcg aagatggcga aagcgaagct acgaaaatgt acggatcgca
     2101 tggaagcctc ctgcggcgtg cgcgtagtcc ggtaacgatc gtgcccgact attgcggtgt
     2161 tgacaatagg tcgcacgtca tccgtgactt tcgggtcatc agcgcgtggt cttcccggtg
     2221 cccggtgccc tgcgcaaggc gcgacccgag ctcgaagaac caatcatcgg ctgctctggc
     2281 cgtggccgcc tcctgccgcg ctttcgctag aatggaccga tgttccgaat gggcgatgcc
     2341 atgcacaccc tcgtgatgat ccgccatggc cagtcgctgt ggaaccagga aaaccgtttc
     2401 accggttggg tcgatgtcga tctttccgag cagggctggc gcgaagcccg cgaagccggt
     2461 gaactgctga agcgggaagg ctacggcttc gacatcgccc acacctcggt gctgacgcgc
     2521 gcggtgcaca ccttgtgggg cgtccaggat gcaatggacc tcgcctggtt gccggtggtc
     2581 accgattggc gcttgaacga acgccactac ggtgcgttgg ccgggctcaa caaggccgag
     2641 gtcgccgcca ggcacgggga cgaacaggtg cacgtctggc gccgcagcta cgacgtgccg
     2701 ccgccgccgc tcgatccgtc cgagaatacc ttcgcgcacg atccgcgcta cgccaagctc
     2761 gatcccgcgc agattccgtt gacggagtgc ctgaaggaca ccgtggcgcg ggtgctgccg
     2821 tactggcacg aggtgctggc acctgcgatc cgatccgggc agcgggtgct ggtgtccgcg
     2881 cacggcaatt cgatgcgggc gctggtgaag tacctcgacg ggatttcgga cgccgacatt
     2941 cccgggctca acatcccgaa cggcgtgccg ctggtgtacc gcttcgacaa cgcgctgaca
     3001 ccggtcgaac acttctatct gggcgacgcc gatgcgatcg cggccaaggc ggccgcgatc
     3061 gccaaccagg gacgggccaa cgcgggctaa accgggtttt attccttcgc gcgactccac
     3121 ggccaggcga agacgaagct cgcgacgccg atcaccgcga gcacgatcgg cggccagatg
     3181 ccgtagcggt cgcccagcgc gaacaccagg cccgcgccca cgagcagcgc gccgccgaac
     3241 gccgccgcca ggatccgtcg ctggacctcg cgttgcagca tgcgcaggtg ttcgagttcg
     3301 cgttcgccga actgcagcgg cccgttgccc cgggccagcg cttccagtgc gtcgtggatc
     3361 ttctccggaa tcagcggcgc gatgcgcagc cactcgggga ttcgcttgcg catttccctc
     3421 agcgcatggc gggcgctgta gcggtcggcg aggatccgct ccaggaccgg ttgcgccacc
     3481 gaccagatgt cgatgcccgg atcgacctgg cggcccagcc cttcgacgtt cagcagggtc
     3541 ttttgcagga ggatcaattg cggctgcagg gtcagttcga acttgcgcgc cacgctgaac
     3601 aatttcgcca ccacctcggc gagcgagatt tccgacagcg gacgcgtgaa gtagggctcg
     3661 cagaccgtgc ggatcgccgc ctcaagttcg tcgaccctga tggatcgggg catccagccg
     3721 gcggcaatgt ggagttccgc gatgcggtga tagtcacgct cgaacatcgc gatgaagttt
     3781 tccgcgagcc agtactggtc ctcttcgggc aacgagccca tgatcccgaa atcgagcgcg
     3841 atgaagcgcg gctcgcgcga gcgcgcggga tcgacccaga tgttgcccgg gtgcgcgtcg
     3901 gcatggaaga agttgtcgcg gaatacctgc tcgtagaaca gccgtatgcc tttttcggct
     3961 agtacccggc gatcgatgcc ggccttgtcc agcgcggcga catcgtcggc gggaatgccg
     4021 tagacgcgct ccatcgtcag caccttctcg caggtatgcg accagaacac ctcgggcacg
     4081 tacaggtcgt cggaaccctc gaagttgcgg cgcagcaggc tggcgttggc gccctcgttc
     4141 tggaggttca gttcgttgtg cagggtgcgg gcgacttcgt tgaacatgtc gcgcggacgg
     4201 atcttgtcgg cctgcggatg ccagcgctgc accagccagg ccagcgcatc cagcagctcg
     4261 aggttctgcc ggatgttctt gcggatgttc gggcgcagga ccttgacgat gacttcgcgg
     4321 tccccgacga atgccgcggc cgtgggccgc tccgcattcc cctcgcccgc ttgcgggagg
     4381 ggatgatggc gcgaggcgcc cggtgggggc ctgctcgtgg gggctctcgg gtgcagccgt
     4441 gcagcgtgca cctgtgcgac cgaggccgag gcgagcggcg tctcgtcgaa gctcgcgtac
     4501 agctcgccga tcggcttgcc gagcgactcc tcgacgatcg cgcgcgcctc ggcgccggga
     4561 aacggcgtga cccgatcctg gaggtgcgcg agttcttcgg cgacgtcctc gggaatcagg
     4621 tcgcggcggg tcgaaagcac ttgccccgcc ttgacccaga tcggaccgag ctccgtcagc
     4681 gcggcacgca gccgcgcacc gcgcggcagc gccgcgatcg gtttgcgcgg gcgcggcagg
     4741 aacacgcgca gccagcgcag cggccgcaac gcatgttgct cgtcgatcag gtcgtcgacg
     4801 cggtaacgca ccagtaccgc ggcgacgtgc accacgcgcg gcaacaggcg cagcggggtc
     4861 atggcttgtc gttccgcaag cgcgccatgc gcgccgccaa ccgttcggtg cgttcccgca
     4921 agtcgtcgac atcgtccagg aaggcgttca tttcggggtg cgggacggtg acgcgggctt
     4981 cgtcacgcag ccagtcgatg gtgtcgtcgc ggaaatggcg cgcgccctcg ccggcccatt
     5041 tcgtggcatc gcgcagcgca cgcgcgatcg tggtaccaac gacttcgccg aataccgccg
     5101 tgaacgccgc ttcgaaatcg ggccggtact cgcgcgcgag tttctggacc tggcgcgcga
     5161 gctcggcgtc gcccgcgatt tccaccttgc cgggcggacg agcatcgccg tcgcgcccca
     5221 gcaccatcgc gagcaacgac cccggcgtcg ccttgacctc gaagtcgctg gcgctgccgt
     5281 ccagtcccgc ttccggcggc ggctcgattt ggagcgtgct gttgcgcgcc gcgagcgcga
     5341 aggcgagctc cggcccgcgc aggtagacgc cgatacgacg gccttccagg cgcccgatgg
     5401 cctgccgcgt ttcggggtcc agcgccacca gccggttcag cgcgagagtg agggcgaggc
     5461 caccaagctt gcgaagcggg tggggaatcc aggcgttcgg ttcgcgttcg ggcatcgcgt
     5521 cattgtatgc gatcgtccct gatcgccgcg ttggccgtgt cacctcatcc ggcatgcgtt
     5581 gcgttcatgc ggcaccagaa cgggcatcct gccctgactt tccacgacag cggctcacgt
     5641 tgccaagcat ctctgcaacc gctaccctgc gcgcactccg tcggggagta gccgcttccg
     5701 gcatgacgcc ggaaggcttg cgtcaacaca ctcggccccc aggccgtggc gcaggcagtc
     5761 cgcgaggcag gcgagaccga cagtcgcgat gggcgtgtcc gggccggaac gcgcgtcggg
     5821 attgtcgcgc gtcaccggcc ccgggagttc cgattggaag ccttcctcgt ttcgctgggc
     5881 gccgtcgcgc tcgccgagat cggcgacaag acccaactgt tgtcgctggt gctggcggcc
     5941 aagttccgca agccgtggcc gatatgcggt ggcatcctgg tcgcgtcgct gctcagccac
     6001 gcgatcgccg ccgaactcgg cgcctggctc gcgcactgga tgacgccgga agtgcagcga
     6061 tggttgatcg ggctctcgtt cctggcggtg gcggcgtggg cgctgctccc tgaaaaggag
     6121 gagccggcag atgctccgcc gcggcgcggg cacggcgtgt tcgtcgtatc ggtcatcgct
     6181 tttttcgtcg ccgaacttgg cgaccgcacc caggtcgcga cggtagtgct cggggcgcac
     6241 taccagcccc tgtggcaggt catcgccggg agcactgcgg gaatgctcgt cgccaacgtg
     6301 ccggtcgtgt ttctcggggc gcgtttcgcg cacaagcttc cattgcgcgc cgcgcgactt
     6361 gcagccgccg ccttgttcgc cgcgctcggc gtgtggatcc tgttgcgttg aagcctagcc
     6421 tcctccggga aacccgagct gtcgccatgc ttcgtacacc gcgatcgcga ccgcattgga
     6481 caggttgatg ctgcggttgt ccggttgcat cggcaggcgc agccgccgtg gctcgggaat
     6541 cgtctgcagc accgcttccg gtagtccgtt cgtctcgcga ccgaacagaa gggcgtcgcc
     6601 atcggcgaaa cgcgtgtcgg tgtaccgatg ccgcgcacgg ctggtgaacg cgaagacgcg
     6661 tggttgctgc agggcctcga ggcacgactc gaaatcatcg tgcgccatca cgttcacgaa
     6721 ctcgtggtag tcgagcccgg cgcggcgcag cttggcgtgg tcgagctcga aaccgagcgg
     6781 ccggatcagg tgcagcgacg tgcccgcatt cgcgcacagg cggatcgcgt tcccggtgtt
     6841 ggggggaatc tggggttggt acaggatgac gtgcagcacc ggggcattat cgccgcccgc
     6901 gcgcgctcct cgtgcgccgc ggacacaggc gggaagtccc ccgtggcctg gtcagtgcag
     6961 gctcatcagg atgatgccgc acaagaccgc gagcgccgcc atcgcgaaca tgccgcgcat
     7021 gagtactgat tcgactttca tgtccctgtc ccctgtcgcc ggagttcccg gcacgggatt
     7081 cgaagcaatg cccatgccac ggagccggcc gcgcggcgcc tgatttgccg aacccggcct
     7141 gcggcgggcc cggaatgtga cgcagttcac gctgccggcg ggccgcaagg gagccggacg
     7201 ggcgccagct accagggcag cgctgcgccg tcgtaggcca ggaacgtgcc cgcttcctcg
     7261 tttccggcac gctccacgag cccgagcagc gcatgggcgg agtccgcagg cgaaagcgtc
     7321 gcgcccttgc cgcccatctc ggtcttcagc cagccgggat ggacggcaag caccacgatc
     7381 cctcgcggcg tcagctccgc cgcgagccgt ttcaccgcca tgttgagggc ggccttgctc
     7441 atcgcgtagc tcagggtgcg aaaacgggcc gactgcgcga tcgaggcgat ctgtgaggtg
     7501 acgcagagca cgcgcggctt gttgccgagc gccagcaacg gcgccaacgc ctgggtgatc
     7561 aggagcggcg cgctggcatt gaccgcgaaa ctctccgcca gcgactccgc cttgacgttg
     7621 ccgaagcgct cgcccgacac cagcaccccg gcgttgttca ccaacaggtc gatgcgttgc
     7681 aggtgccttg ccgccactgc aggcagcgcc tcgatcgcct gtgcgtcggc gacatcgagc
     7741 ctgtggatta cgagttcgtc ggcgtgggct gcgcgcaggg catcgagcac ctgggcgtgc
     7801 gccgggtcac ggcagcaggc catgacgcgc gcaccgcgtt ccagcagttc gctgacgaag
     7861 gccaatccga gtccgcgatt ggcgccggtg acgaggcagc gttgcatggc ggcttccctg
     7921 gcttcctcca aaacaaggcc atggacggcc ggggtggagt tgacaactgg cgaaccggca
     7981 cgctcactca accgcgcgac gaagcagcga gcacggatgc gagcaaatca aagtgtaacc
     8041 cgggccgcgc cgaccgtgcc ttgtggccga ttgctttgtg gaatggctcc ggctacggtt
     8101 tcgtgacaat caatggtccg gggaggactc cacatgcgct cgcactggtt ggcgtggttc
     8161 gcagtgctcg gtttttcact catcgctgga gcggcccagg ccgccgctgc cgctccgaag
     8221 ctcgacatcg acatccccta cacccgcttc acgctgccga acggcctcac cgtcgtggtc
     8281 agcgaggacc acaaggcgcc gatcgtcgcg gtcagcgtgt ggtaccacgt cggctccggc
     8341 tacgagcccg aaggcaagac cggcttcgcg cacctgttcg agcacctgat gttccagggc
     8401 tcggagcacc acaagggtga atacttccag ccattcgaac tggtcggtgc caccggcatg
     8461 aacggcacga cctggctcga ccgcaccaac tacttcgaaa ccgtgccgac cacggcgctc
     8521 gacatggcgc tgtggatgga atccgaccgc atgggccacc tgctcggcgc gatcggccag
     8581 ccggaactcg acgagcagcg cggcgtggtg cagaacgaaa agcgccaggg cgaaaaccag
     8641 ccttacggcc gcgccttcga ggacatccag gcgaacgcct tcccggccaa ccatccctac
     8701 caccacgaca ccatcggttc gatggcggat ctcaatgccg cttcgctcga caccgtgaaa
     8761 cagtggttca aggactacta cggcgcggcc aacaccacgc tcgtgctggt gggcgacatc
     8821 accccggcgc aggcccgcga aaaggccatg gagtacttcg gcgacatccc ggccggcccg
     8881 cgcgtgccgc ggccgcaggc atgggtcacc gcgcgtgaca cctcgacgtc gggcacgatg
     8941 accgacaacg tcgcgcaggt tcgcatctac cgcgaatgga acacgccggg gctggatgcg
     9001 gagggcaacg atcgcctgat gctggatctc gccgcgcagg tgcttggcgg cagcaagacc
     9061 tcgcggctgt accagcggct ggtctacaag gacaagctgg ccgacgacgt gtcggtcggc
     9121 aacatgacct tcgaactcgc aaacatgacc ttgctgcagg tcgacgtgaa gaagggcgtc
     9181 gatccgaaga aagtcgaggc cgcgatcgcc gacgaatggt cgaaattcct gaaggacggg
     9241 ccgaccgccg acgaactgga acgcgtcaag gccacctacc aggcgtccac catccgcggc
     9301 ctggaacgga tcggcggatt ctcgggcaag gcggtgacgc tggccgaagg ccaggtctat
     9361 cgcggcgatc ccggcgcgtg gaagaaggat tacgaagtca cgatggctgc aaccgcggcc
     9421 gaagtgaagg cggccgccga caagtggctg tccaagggcg actacacgtt gaccgtggtg
     9481 ccgggcaagg tcgaggcgga ggacgccgcg acgatcgccg gccgcgccgc gatcccgggc
     9541 cgtccctccg accagctcac gcccaaggcc gactacaagg tcacggaggg cgacatcgac
     9601 cgcagcaagg gcgtgccgac ggtgacggcg ttccccgacc tcaccttccc ggaactgcag
     9661 cacggcaagc tcaagaacgg catcgaggtg gtgctggccg agcggcactc gatccccgtg
     9721 gtgcaggtcg aggcgctgtt cgacgcgggt tacgccgcgg accagggccg caagctcggc
     9781 acctccagtt tcacgatggg catgctggac gaaggcacca gggacctcga ctccgtcgag
     9841 atcgccaagc gcaagcagcg cctcggcgcc gagatcgcca gcgggtgcga cctcgacatc
     9901 tgcaacgcgt ggatgagcgc gctggacgcg aacctcgccg actcgctggc gttgtacgcg
     9961 gacatcgtgc gcaacccggc cttccgggat gccgacgtcg cccgcctgcg cggccagtgg
    10021 ctcgccggca tcgcgcagga gaagacccag ccgaccgcgc tggctcagcg actgctgccg
    10081 ccgatcctgt acggcaaggg caacgcctac gcgatcccgt tcaccggctc cggtaccgag
    10141 gcctcgatca agtcgctgac cacggatgac atgcgcgggt tcgtccacga ctacatccgc
    10201 ccggacaaca tgaagatcct ggtggcgggc gacaccacgc tggatgcgat catcccgcag
    10261 ctcgataagg tgttcggcga ctggaaggcg ccggcgtcgc cggtcccggc caagaacctc
    10321 gccaaggtcg agtacccggc gaagtccagc gtgtacctgg tcgatcgacc cggcgcgcag
    10381 cagtcggtga tcatcgcggg actggtcgca ccatccacca aggcgcccaa caacctcgag
    10441 attggcacca tgaacggtgc gttcggcggc accttcacct cgcggctcaa catgaacctg
    10501 cgcgagggca agcactgggc ctacggcgcc ttcagcttca gccccagcgc gatcggccag
    10561 cggccgttcc tgatgtacgc gccggtgcag accgacaaga ccgcaccgtc cgccgaggaa
    10621 gtgctgaagg aagcccgtgc ggtggtgggc gacaagccgc tcaccgacaa ggagatccag
    10681 aagatcaagg acaacgacat ccgcaagatg ccgggccagt acgaaaccgc gcaggcggtg
    10741 ttgaacgcga tggagggaat cgtcaagtac gaccgtcccg acgactacgt gcagacgttg
    10801 aagggccgga tcgaggcgca gcaggatggc gaagtgaatg ccgcggcgaa ggaagtgatc
    10861 catccggcac gcctgacctg ggtgatcgtc ggcgatcttt ccaagatcga gaagccggtg
    10921 cgtgcgctcg acctcgggga agtccacgtg atcgatgcgg acggcaaccc cgtgaaatga
    10981 atccggcccg ccggagcgca gtccactcgc ccgccaggtg ccgtgcacct ggcgggtttt
    11041 tcttccccct tcggaacgac ggcggagcga agcgaacgcg cacgtagtgc gatgaggggg
    11101 atggcttttg catcattggc gctgcgcttc attgcatggc ttggccccgc cgctggtgtc
    11161 gatgcccggc catcgcctgt gcctgcgtta ccatgcgggc atgagagaaa cgcgtatccc
    11221 cggcgccaca acccacttcg gctaccgcga agtgcccgtc gccgaaaaac agaggctggt
    11281 cggcgaagtg ttctcgtccg tcgccggcaa atacgacgtc atgaacgacc tgatgtcgtt
    11341 cggcgtgcat cgcttgtgga agcgattctt cgtcgcgacg tcgggcgtgc gtcgcggcga
    11401 ccgcgtgctg gacctcgccg gcggcaccgg cgatatcgcg gccctgctgc tgccgcgcgt
    11461 cggcaagcag ggcgacgtcg tcgttgccga catcaacgcc gcgatgctgc gcgcgggacg
    11521 cgatcgcctg ctggatcgcg gcctgctccg gggcctgcga ttcgcgcagg tgaacgccga
    11581 ggcgctgccg tttccggatg cgtccttcga tgcggtgacg atcgcgttcg gactgcgcaa
    11641 cgtcaccgac aaggacaagg cactcgcgga aatgcaccgc gtgctgaagc ccggcggccg
    11701 ggttttgatc ctcgaattct cgaaggtcaa ccaggactgg ctgaaaccgc tgtacgactt
    11761 ccattcgttc caggtgctgc cgcggatcgg caagctggtc gcgcacgatc gggacagcta
    11821 ccgttacctc gccgaatcga tcaggaaaca tcccgaccag gcgacgctca aggcgatgct
    11881 ggaagccgcg ggttttcgca acgtcgaagt gcgcaatctt tcggccggga tcgtggcgat
    11941 ccatcgcggc taccggatct gaatcgccgc catgcaatcc gaacctcgct caccgctcga
    12001 actgaatgcc gcgatgcgca tcgtttctgc gattgcagcc gcttccgttc tcgtcggtct
    12061 tgtggtggcg ccgtacaccg acaggctacg aatggccagc aatgccgttt tttgcgtggt
    12121 tctggcctgc tgggcgatcc acggggatcg cacgcggcgc aatcgcatgc tgacggcgct
    12181 gctgttgctg atcttcctgg cgattttcgc gttgcgggtt cgggagcacg gctttccgtg
    12241 acatcgtagg caaacggcga gggactcgtg cgagagctgt accccgaaat cgagccgtac
    12301 cgtaccgggc gttttccggt ctccgaccgg catacgttgt atctcgagga atgcggcaat
    12361 cccgacggcc tgccggtggt gttcctgcac ggcgggcccg gcgcggggct cgcgcgttac
    12421 caccggcgat tcttcgatcc cgcgcgctgg cgcgtcgtgc tgttcgacca gcgcggtgcg
    12481 ggacggtcca cgccgttcgc ggacctcgag gacaacacca cccaggaact cgtggacgat
    12541 atcgagaaga tccgcgcaca cctcggcatc gttcgctgga tggtgttcgg cggttcgtgg
    12601 ggttcgaccc tcgcgctggc ctatgcggag gcgcacccgg agcgcgtgtt cgcgctggta
    12661 ttgcgcggga ttttcctcgg ccggccggag gaaatcc
//
LOCUS       DASSNH010000070         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_104621, whole genome shotgun sequence.
ACCESSION   DASSNH010000070 DASSNH010000000
VERSION     DASSNH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104621"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFK00_02600"
     CDS             complement(<1..489)
                     /locus_tag="VFK00_02600"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HET7591874.1"
                     /translation="MALTPPFNLQRWIDEHRHLLKPPVGNKCIVDGDFIIMIVGGPNS
                     RSDYHWDEGPEFFYQLEGEMVLRVQDDGQRRDLPIRAGEMFYLPPRTPHSPQRMRDSI
                     GLVIERRRLSHEKDGLLWFCEKCNHKLYEEYFTLGSIESDFPPVFERYYGSLEARTCK
                     QCG"
     gene            674..1225
                     /locus_tag="VFK00_02605"
     CDS             674..1225
                     /locus_tag="VFK00_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7591875.1"
                     /translation="MSADTAMLAVDIQQSFLRAPYWRDDDVPAFRAATLRVLHAAAER
                     GWPVVQILHQESNDGPFGPAAGLVQPLEWMPENVAVTFRKGVHNAFTDTGLEVWLRDR
                     GIRRVVVIGIRTEQCCETTTRVASDLGFDVDFVTEATLTFPMVHAASGRRYDVDEIKQ
                     RTELVLDGRFARIRGVDALLADD"
     gene            1173..2165
                     /locus_tag="VFK00_02610"
     CDS             1173..2165
                     /locus_tag="VFK00_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7591876.1"
                     /translation="MDALPASAASMPCSPTTDVWFVLPPGVVLLDFAGAAEAFRIAAS
                     FGARFRLRTTAAIDSGQMVDTSIGPGLSGVEPLPERVEPGALLVVCGVAGDAAAHDSH
                     GTQAVTSWLRRLTPLPRLACVCSGALIAARAGLLEGRRCTTHHTLIAALRRIAPHAEV
                     LDSRVFVEDGNICTSAGITAGIDLALHLIAQMTSPRLSVEVAREMVVYLRRAPDDPAL
                     SPWLEGRNHMDARVHQVQDLLARAPQRDWTLAQLAGLGHMSVRTLTRRFRDSAGMSVH
                     DYHARLRVALARQALASGSSVEAAAESAGIGSARQLRRLWKTHADATPGTHRRS"
     gene            complement(2394..>2536)
                     /locus_tag="VFK00_02615"
     CDS             complement(2394..>2536)
                     /locus_tag="VFK00_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591877.1"
                     /translation="TRRLVQLTVDDNDATSKLMDMLLAKKRASDRRNWLEEKGDLASL
                     EV"
BASE COUNT          402 a          831 c          835 g          468 t
ORIGIN      
        1 cccgcattgc ttgcaggtgc gcgcttccag agaaccgtaa tagcgctcga acaccggcgg
       61 aaaatcgctt tcgatgctgc cgagggtgaa gtattcctcg tagagcttgt ggttgcattt
      121 ttcgcagaac cacaaaaggc cgtccttttc gtgggacagg cgtcgccgct cgatcacgag
      181 gccgatcgag tcgcgcatcc gttgcggcga gtgcggcgtg cgcggcggca gataaaacat
      241 ctcgccggcg cggatcggca ggtcgcggcg ctggccgtcg tcctgcacgc gcaacaccat
      301 ttcgccttcc agttggtaga agaattccgg gccctcgtcc cagtgatagt cgctgcgcga
      361 attcggaccg cccacgatca tgatgatgaa gtcgccgtcg acgatgcact tgttgccgac
      421 cggcggtttc agcagatggc ggtgttcatc gatccagcgt tgcaggttga acggcggcgt
      481 cagcgccatg gtccgattct cgcgggagcc aagtcgccac catagcgccg ccgcgaccac
      541 gcgcaaagcg ggagcgccgc tgccccgcgc cggcgcgcat ggcccgatcc gtccgcatgc
      601 ggtccgggtt ggtgctcgcc ggcggcattc cggctgccca caatgacgcc tcccgatccg
      661 atggaatttg tccatgtctg ccgataccgc gatgctcgcc gtcgacatcc agcaatcctt
      721 cctgcgcgct ccatattggc gcgacgatga cgtaccggct ttccgcgccg ccacgctgcg
      781 ggtgctgcac gctgccgcgg aacgcggctg gccggtggtg cagatcctgc accaggaaag
      841 caatgatggc ccgttcggcc ctgctgcggg gttggtacag ccgcttgagt ggatgccgga
      901 aaatgtggcg gtgaccttcc gcaagggcgt gcacaacgcg tttaccgaca ccggcctgga
      961 agtctggttg cgcgatcgcg gcattcgtcg cgtcgtcgtg attggaatcc gcaccgagca
     1021 gtgttgcgaa accaccacgc gcgtcgcttc cgacctcggc ttcgatgtcg atttcgtgac
     1081 cgaggcaacg ctgacctttc cgatggtcca cgccgccagt ggtcgccgct acgatgtgga
     1141 cgagatcaag cagcgcacgg aactggtgct cgatggacgc tttgcccgca tccgcggcgt
     1201 cgatgccctg ctcgccgacg actgacgtct ggttcgtgtt gccgcccggc gtggtcctgc
     1261 tggatttcgc cggcgcggcc gaggcgttcc gcatcgcggc cagcttcgga gcacgctttc
     1321 gcctgcgaac gacggcggcg atcgattcgg gccaaatggt cgacacttcg atcggtcccg
     1381 gtttgtccgg ggtcgaaccc ttgccggagc gggtcgagcc cggcgccctg ctggtcgtgt
     1441 gcggcgtggc gggtgacgcc gccgcgcacg attcgcacgg aacccaggcg gtcacctcct
     1501 ggttgcggcg gctcacgccg ctaccgcgcc tggcctgcgt gtgttcggga gcgctgatcg
     1561 ccgcacgcgc cggactgctg gaaggccggc gctgcaccac ccaccacacg ttgatcgcgg
     1621 cgctgcgcag aatcgcgccc catgccgagg tgctggacag ccgcgtgttc gtggaggacg
     1681 gaaacatctg caccagcgcc ggcatcaccg ccggcatcga cctggcgttg cacctgatcg
     1741 cgcaaatgac gtcgccgcgg ctttcggtcg aagtcgcgcg ggagatggtc gtgtacctgc
     1801 ggcgcgcgcc cgacgatcct gcgctgtcgc cctggctcga aggccgcaac cacatggacg
     1861 cgcgcgtcca tcaggtgcag gatctcctgg cacgcgcacc gcagcgcgac tggacgctgg
     1921 cgcaactggc cgggctcggc cacatgagcg taaggacatt gacgcgacgt tttcgcgact
     1981 cagcgggcat gtcggtgcac gactaccacg cgcgcctgcg cgtcgcatta gcgcggcagg
     2041 cgctggcgag cggaagcagc gtcgaggccg ccgcggagag tgcaggaatc ggatcggcac
     2101 gccaattgag gcgattgtgg aagactcacg ccgacgctac gccaggcacg catcggcgct
     2161 cgtagagaga atggtcgcaa cgtcgccagc gaaacaggat ttgcctgtca ttccggcgaa
     2221 agcccgaatc cgggtttctg cgtgcaagta aagcaagcgc gaaatggatg acaggccgcg
     2281 acggcggccc gttgagccat cggcttcgcc ggtgtccgct ccggcatcct gccatcgcgg
     2341 cccggctttc actgggatgg cgatgggttt gtgttcccgt gtccctggct tcctcacact
     2401 tcgagcgacg ccagatcacc cttttcttcg agccagttgc gtcggtcgga tgcgcgtttc
     2461 ttggccagca gcatgtccat cagtttcgac gtcgcatcgt tgtcgtcgac ggtcagttgc
     2521 accagccgcc gcgtgt
//
LOCUS       DASSNH010000071         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1581230, whole genome shotgun sequence.
ACCESSION   DASSNH010000071 DASSNH010000000
VERSION     DASSNH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581230"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1183
                     /locus_tag="VFK00_02620"
     CDS             248..1183
                     /locus_tag="VFK00_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET7591878.1"
                     /translation="MPAALICGSMAYDTIMVFDDQFKNHILPDKVHMLNVSFLVPRMR
                     REFGGCAGNIAYNLKLLGGEPIPMATVGQDFGPYREWFEGLDIRLDRVREVPGTFTAQ
                     AFITTDRDNNQITAFHPGAMMESHLNHAGEVAEAAIGIVAPDGREGMLQHAVEFAARG
                     TPFIFDPGQALPLFSGEEFRAFIEQADYVAVNSYEASLLSERTGWSEGEIAERVKAYI
                     VTHGAEGSTIHAGGKSHDIPPAKPREVVDPTGCGDAYRAGLIHGILAGMDWPNVGRVA
                     SLLGAIKIAHPGTQNHRFDPDGFADEFKAQFGHEL"
     gene            1310..1750
                     /locus_tag="VFK00_02625"
     CDS             1310..1750
                     /locus_tag="VFK00_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591879.1"
                     /translation="MSRAPMLALVSLLAGAPLLYAGNASAANEITCRMTFELHGWSAF
                     YKKADGSGQVSCSNGQHMQVNLRSRGGGLSFGKSNIDGVGKFSGIYDISEILGTYVVG
                     EAHAGAANSARGTVMTKGNVSLALSGTGKGWNLGVDFGKFTISK"
     gene            complement(1871..>2209)
                     /locus_tag="VFK00_02630"
     CDS             complement(1871..>2209)
                     /locus_tag="VFK00_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET7591880.1"
                     /translation="LLRAARAFLDPMLNLGLITRQRAHDILVDDVGISEAFAREELDR
                     FMFRSPGQATAYFYGYSRILELRAHTEIALGDKFDLQQFNDFLLAQGLLPPDQLAKAV
                     ETKFIPSHQK"
BASE COUNT          423 a          720 c          730 g          336 t
ORIGIN      
        1 accgaggtcg atcgaaaggt cgttggagaa gatgccgcgg aacagtttga acatcgcgag
       61 cccgagacgt atggcgggta ggcagaacgc gccagtctag tcagcagggg gatgccgcgc
      121 aaggacggaa tcctcaaaat cacgcgccgc catgcgccat tcgggggccc ggcggcgggt
      181 gcgctcgaaa ccgcccggcg caaccgttac gatcacgccc ttgtgcctgc cgaaagggaa
      241 ccaaccgatg cccgctgcgc tgatctgcgg ctccatggcc tacgacacca tcatggtgtt
      301 cgacgaccag ttcaagaacc acatcctgcc ggacaaggtg cacatgttga acgtgtcgtt
      361 cctggtcccg cggatgcgcc gcgagttcgg cggatgcgcc ggcaacatcg cctacaactt
      421 gaagctcctc gggggcgagc cgatcccgat ggcgacggtg ggccaggact tcgggcccta
      481 ccgcgaatgg ttcgaggggc tcgatatccg cctcgaccgg gtccgggaag tgccgggcac
      541 cttcacggcg caggctttca tcaccaccga cagggacaac aaccagatca ccgccttcca
      601 cccgggcgcg atgatggaga gccacctgaa ccacgcgggc gaggtcgcgg aagccgcgat
      661 cggcatcgtg gccccggacg ggcgcgaggg gatgctccag cacgcggtcg agttcgccgc
      721 ccgcggcacc cccttcatct tcgatccggg gcaggcgctg ccgctgttct ccggcgagga
      781 attccgcgcc ttcatcgaac aggccgacta cgtggccgtg aacagctacg aggcgagcct
      841 gctcagcgag cgcaccggct ggtccgaagg cgagatcgcc gaaagggtca aggcctacat
      901 cgtgacccac ggcgccgaag gctccaccat ccacgctggc ggcaagtccc atgacattcc
      961 acccgcgaag ccacgcgagg tggtcgatcc gaccggctgc ggcgacgcct accgggcggg
     1021 gctgatccat ggaatccttg cgggcatgga ctggccgaac gtcggccggg tggcctcgtt
     1081 gctcggggcg atcaagatcg cccacccggg cacccagaac catcgcttcg atccggacgg
     1141 attcgccgac gagttcaagg cacagttcgg ccacgagctg taggcgcatc gtcaaagctg
     1201 aacgcggcac cgcgaaacct gaacgtgcgt ggcggcgtca catgacggag acggggcggc
     1261 gcgcaagaat gcctccaact cgtctcacgc gaccagaagg agaagcccca tgtcacgcgc
     1321 acccatgctc gccctcgtct cactgctcgc cggcgcgcca ctgctgtatg ccggaaacgc
     1381 cagcgccgcc aacgaaatca cctgccggat gaccttcgaa ctccatggct ggtcggcgtt
     1441 ctacaagaag gccgacggaa gcggccaggt gagctgcagc aatggccagc acatgcaggt
     1501 caacctgcgc agccgcggtg gcggcctcag cttcggcaaa tccaacatcg acggcgtcgg
     1561 caagttctcc ggcatctacg acatcagcga gatcctcggc acctacgtgg tcggcgaggc
     1621 ccatgcgggc gcggcgaatt ccgcgcgcgg taccgtgatg accaagggca acgtgtccct
     1681 ggccctgagc ggcaccggca agggctggaa tctcggcgtc gatttcggga aattcaccat
     1741 cagcaaatag gcggacaacc cgccgatact gcggggcggt gtccgcccgc aggcccgcaa
     1801 tcgtgcagga gcggccgatg gccgcgatcg cccgatgttg cgacggtcgc gggcatgggc
     1861 cgccctactt tcatttctgg tgggacggga tgaatttggt ctcgactgcc ttggccagtt
     1921 gatccggcgg aagcaagccc tgcgcgagca ggaaatcgtt gaattgctgc aggtcgaact
     1981 tgtcgcccag cgcgatctcg gtgtgcgcgc gcagctccag gatccgtgag tagccgtaga
     2041 aatatgcggt ggcctgtccc ggcgagcgga acatgaagcg gtcgagttcc tcgcgcgcga
     2101 aggcttcgga gatgccgacg tcatcgacca ggatgtcgtg cgcgcgctgg cgcgtgatga
     2161 ggccgaggtt caacatcggg tcgaggaacg cgcgcgccgc gcgcagcag
//
LOCUS       DASSNH010000072         7000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_671929, whole genome shotgun sequence.
ACCESSION   DASSNH010000072 DASSNH010000000
VERSION     DASSNH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671929"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFK00_02635"
     CDS             complement(<1..295)
                     /locus_tag="VFK00_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET7591881.1"
                     /translation="MRTSWYRMPRLLLSLALPAAIAACAPAPIYKTGGPSSVMATPQQ
                     VATSPANFRNLEVVWGGEVIGVHNLADHSEVEILAYPLDSSQRPRLKEPPTGRF"
     gene            complement(373..1215)
                     /gene="rlmJ"
                     /locus_tag="VFK00_02640"
     CDS             complement(373..1215)
                     /gene="rlmJ"
                     /locus_tag="VFK00_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HET7591882.1"
                     /translation="MNYRHAFHAGNFADVFKHAVLLALLDALTAKDKPLCYFDSHAGR
                     GAYRLDDAEALKTGEWRDGIGRLFGNTQAPAPLQRYVAAVRTCNPDGVLRTYPGSPLL
                     AARALRAGDRLVLCEMQDDEAAALRALFRDDGRAHVHRRDGYAALNALLPPGERRGLV
                     LIDPPFEAQEGEFGAIEATLAKAHARWPNGVYAVWYPIKSHRAIAPFHRHLSQGPFER
                     VLVAELMVLPDDSPLRLNGCGMLVANPPWQVDEALAGLLPALRAVLAQSPRASQRVRW
                     LCGT"
     gene            1350..1586
                     /locus_tag="VFK00_02645"
     CDS             1350..1586
                     /locus_tag="VFK00_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HET7591883.1"
                     /translation="MNTRGQEHVEAIRKSTITCPFCGHRDVETMPENACVFFHPCAGC
                     GKLLRPKPGDCCVFCSYGDVPCPPVQRQRRCCDR"
     gene            complement(1601..2347)
                     /gene="msrA"
                     /locus_tag="VFK00_02650"
     CDS             complement(1601..2347)
                     /gene="msrA"
                     /locus_tag="VFK00_02650"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7591884.1"
                     /translation="MKTPMISVTLRTLFVLLVIPAVASCAPLTDAGAAAHTAVPVPKV
                     DAPLASRSGNATAVLAGGCFWGMQWVFEHVPGVVNVTSGYSGGAATTAAYGQVSEGDT
                     GHAEAVRITYDPSRVTYGQLLRVYFSVATDPTELDRQGPDTGTQYRGVVFYADPEQQE
                     VAKAYIAQLGAAHVFARPIVTQLVPLKAFYPAENYHQDYARLHPDALYIAINDAPKVA
                     ALQRELPDLYRAQPVVWHDATSNVITVDLH"
     gene            complement(2432..3598)
                     /locus_tag="VFK00_02655"
     CDS             complement(2432..3598)
                     /locus_tag="VFK00_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET7591885.1"
                     /translation="MSPITPDAASRPAPAPLHPFRPLFFCAALFASLGMLAWGAFWHM
                     GWLPPTTLDPLSWHAHGMLYGFGSALVGGFLLTASANWTGIPTTTRRGLLLLTATWVI
                     ARVLLLTPLPLWVGALFDVGYLFGLVFLVARVLWLSRNKRNAFLIGILLVFTGLDIAF
                     HAGALAGNGVVSTRALVGAVDLLMVLMLAIGGRVIPFFTSRKLPGHRSWTHHYLIWAV
                     NGGGAMVLLWDISGVPGMARGALMLAVALFALARLVGWRGWRTWREPMLWVLHLGYLW
                     LAVGLIVRGLALVGVYAMPEIDTLHGITVGALGTLSLAMMTRVAQGHSGVPIRSNAAL
                     TVMFVLPTIAAAVRLCHNQPGGWVWAAALWFAAYVIYLVAIGPLLLRGRVAAAA"
     gene            3748..4164
                     /locus_tag="VFK00_02660"
     CDS             3748..4164
                     /locus_tag="VFK00_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7591886.1"
                     /translation="MRVMVIVKASPESEGGGMPNTEELAAMGKFNEQLVEAGVMLMGE
                     GLHPSSKGARVRFEGKQRTVIDGPFSESKELVAGFWLWQVRSMDEAIEWIKRAPFDGG
                     MEIELRPVFEMDDFGDAVTPEIREREERLRGKIAEA"
     gene            4221..5540
                     /locus_tag="VFK00_02665"
     CDS             4221..5540
                     /locus_tag="VFK00_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7591887.1"
                     /translation="MTTADAHRTIEAVWKIEAPRLIAGLARMLRDVGLAEDLAQEALV
                     TALERWPGDGVPDNPGAWLMTTAKRRAIDQLRHDRMRDDKQAAIGHELQPDAMAHAPD
                     NADALDDDIGDDLLRLIFTACHPVLTRDMQVALTLRMLGGLTTPEIARAFLVPEATVA
                     QRIVRAKRTLAEKKVPFAVPRGEELAARLASVLEVVYLVFNEGYAATAGGDWARPALC
                     EEALRLGRVLAGLAPREPEVHGLVALMEIQASRLHARTAADGSPVLLLEQDRARWDRL
                     LIRRGLASLERAEQLGGADGNYALQAAIAACHARALHAEDTDWTRIASLYARLAEVAP
                     SPVVELNRAVAVSMAEGPEAGLRLVDGLRQERVLRDYHLLPSVRGDLLFKLGRLDEAR
                     AEFERAATLTGNDRESELLRQRAAACGDGMTAVHLPLQGAGGEGDGP"
     gene            5722..6078
                     /locus_tag="VFK00_02670"
     CDS             5722..6078
                     /locus_tag="VFK00_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7591888.1"
                     /translation="MAYMLFVAEPRGQRAERTEEEGKAVYQRMLDYAEDLKARGVLRA
                     YSSLRNDSEGRRLQVRGGKRSLRDGPFAETREMIGGFFLVDCATREEAVELAALCPAA
                     EWATMEVRETGPCYLR"
     gene            complement(6122..6574)
                     /locus_tag="VFK00_02675"
     CDS             complement(6122..6574)
                     /locus_tag="VFK00_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7591889.1"
                     /translation="MSRIATHLALAMLLAAPWRVAMPSSDSQQQQVLAAERAFAASMA
                     NRDLPAFAHHVSGEAIFFTDTGVLRGKQEVIAAWSRLFEGKTAPFSWEPDRVEVLQSG
                     TLALSTGLVRDPDGKVIGRFNSIWRREAPGVWRVVFDKGGPAEPWDRD"
     gene            6771..>7000
                     /locus_tag="VFK00_02680"
     CDS             6771..>7000
                     /locus_tag="VFK00_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591890.1"
                     /translation="MRFQAFATAEALMHWHCRLYLFVLLGLAASPPLAAEPASASCDA
                     PAYRQFDFFVGDWDTYDVQAPGRVIARNTVKV"
BASE COUNT         1210 a         2286 c         2437 g         1067 t
ORIGIN      
        1 ggaagcgtcc ggtcgggggt tccttcaggc gcggtcgctg cgaggaatcc agcgggtagg
       61 ccaggatctc gacctcgctg tggtcggcca aattgtggac cccgatgacc tcgccacccc
      121 agacgacttc caggttccgg aaattggcgg gcgacgtggc gacttgctgc ggcgtggcca
      181 tcacgctgga cgggccgccc gtcttgtaga tcggcgccgg tgcgcaggcc gcgatcgccg
      241 cgggtaatgc gagcgacagc agcagccggg gcatccggta ccaggacgtt cgcatggcca
      301 ttctccgact cggcgcctat gtcgaaggat agtcagaaac ggtctgtgcg ggcgcaccag
      361 ccgccaccgc attcatgtgc cgcacagcca ccgcacgcgc tgcgaagcgc gcggcgattg
      421 cgccagcacg gcgcgcagcg caggcaggag cccggcgagg gcttcatcga cttgccacgg
      481 cggattcgcg accagcatcc cgcagccatt cagacgcagc ggcgaatcgt ccggcagcac
      541 catcagttcc gcgaccagca cccgctcgaa cgggccctgg gacaggtgcc ggtggaacgg
      601 cgctatcgcg cggtgcgact tgatcggata ccacaccgcg tacacgccgt tcggccagcg
      661 cgcgtgcgct ttcgcgagcg tcgcctcgat ggcgccgaat tcgccttcct gcgcttcgaa
      721 cgggggatcg atcaacacca ggccgcgacg ttcccctggt ggaagaagcg cgttcaatgc
      781 ggcgtagccg tcgcgccggt ggacatgggc ccgcccgtcg tcgcggaaaa gcgcgcgcaa
      841 cgcggcggcc tcgtcgtcct gcatctcgca cagcaccagc cggtcgccag cgcgcagcgc
      901 ccgggccgcc agcaggggcg aaccgggata ggtgcgcagc acgccgtccg gattgcacgt
      961 gcgcaccgcg gcgacgtagc gctgcaaggg tgcgggcgct tgcgtgttcc cgaacagccg
     1021 cccgatgccg tcacgccatt cgccggtctt cagcgcctcc gcatcgtcca gccgatacgc
     1081 gcctcggccc gcgtggctgt cgaaatagca caagggcttg tccttggcgg tcagcgcatc
     1141 cagcagcgcc agcaggactg cgtgcttgaa gacgtccgcg aagttgccgg cgtggaaggc
     1201 atggcggtag ttcacgcgcg ggttccgtgt gtcgccgatt cgggcattcg gcgcaaatca
     1261 cctgaaagcc ggttcagggt cgagtgacac gtcgaccatg cgggcgcacg cgcgtggtcc
     1321 tggcaaatcg accggcgtag catggggtcg tgaacacgcg cgggcaggaa cacgtcgaag
     1381 cgatccggaa atccacgatc acgtgcccat tctgcggaca tcgggatgtg gaaaccatgc
     1441 cggagaacgc gtgcgtgttc tttcacccat gcgccggctg cggcaagctg ctgcgcccga
     1501 agccgggaga ttgttgcgtg ttctgctcgt acggcgacgt gccgtgtccg ccggtgcagc
     1561 ggcagcgccg ctgctgcgat cgttgatgcc gccacgagtc tcagtgcagg tcgaccgtga
     1621 tcacgttcga agtggcgtcg tgccagacca ccggctgcgc gcgatacagg tccggcaatt
     1681 cgcgctgcag cgccgcgacc ttgggcgcgt cgttgatcgc gatgtaaagc gcgtcggggt
     1741 gcaggcgcgc gtaatcctgg tggtagttct cggcgggata gaacgccttc aacggcacca
     1801 gctgcgtcac gatcggccgc gcgaacacgt gcgccgcccc gagttgtgcg atgtaggcct
     1861 tcgctacttc ttgttgttcc gggtcggcat agaagaccac gccccggtac tgcgtgccgg
     1921 tgtccgggcc ctgccggtcg agttcggttg gatcggtcgc caccgagaaa tacacacgca
     1981 acagttgtcc gtaagtgacc ctggaagggt cgtaggtgat ccgtaccgct tcggcgtggc
     2041 cggtatcgcc ttcgctgacc tgcccgtatg ccgcggtcgt ggctgcgccg cccgaatagc
     2101 cggaggtcac gttgaccacg ccgggcacgt gttcgaacac ccattgcatg ccccagaagc
     2161 agccgccggc cagcacggcg gtggcgttgc cggaacggct tgccaggggc gcgtcgacct
     2221 tggggacagg cactgccgtg tgcgcggccg cgcccgcgtc cgtgagcggg gcgcacgatg
     2281 ccacggcggg gatcaccagc aacacgaaca gtgtccgcaa agttaccgaa atcattggcg
     2341 ttttcatgtc cgtatcccga acgcctccgg aaaatgatgc cggccgaatg ccgggccaat
     2401 gatacgcctg ccggtggcgg tggcggcaaa atcaagccgc ggcggcgacg cgcccgcgca
     2461 gtagcagcgg cccgatcgcg accaggtaga tcacgtacgc ggcgaaccac aacgctgccg
     2521 cccataccca accgccgggc tggttgtggc aaaggcgtac ggcagcggcg atggtcggca
     2581 gcacgaacat gacggtcagg gccgcgttgg accggatcgg cacgccgctg tggccttgtg
     2641 cgacgcgcgt catcatcgcc agcgacaggg tgcccagtgc gccgacggtg atcccgtgca
     2701 gggtgtcgat ctcgggcatc gcatagacgc cgaccagcgc cagcccgcgc acgatcaggc
     2761 ccaccgccag ccacaggtaa cccaggtgca gcacccacag catcggttcg cgccaggtgc
     2821 gccagccgcg ccagccaacg aggcgcgcca gcgcgaacag ggcgacggcg agcatcagcg
     2881 cgccgcgcgc catgccgggc acgccactga tgtcccacaa cagcaccatc gcgccaccgc
     2941 cgttgacggc ccagatcagg tagtggtggg tccaggaccg gtggccgggc agcttgcggc
     3001 tggtgaagaa cggaatcacc cgcccgccga tcgccagcat cagcaccatc agcaggtcca
     3061 ccgcgccgac cagggcgcgc gtactgacca cgccgttgcc ggcgagcgcg cccgcatgga
     3121 acgcgatgtc gagccccgtg aaaaccagca ggatgccaat caggaaggcg ttgcgcttgt
     3181 tgcgcgacaa ccacagcacg cgcgcgacca ggaacaccag gccgaacagg taaccgacgt
     3241 cgaacagggc ccccacccac aagggcagcg gtgtcagcaa aagcacgcgc gcgatcaccc
     3301 aggttgccgt gagcagcaac aatccgcgcc gcgtcgtggt cggtattccg gtccagttgg
     3361 cagatgcggt cagcaggaat ccgccgacca atgcgctgcc gaacccgtac agcatgccgt
     3421 gggcgtgcca cgagagcgga tccagcgtgg tgggcggcag ccagcccatg tgccagaacg
     3481 caccccacgc cagcatcccg agactggcga acagcgccgc gcagaaaaac aggggacgaa
     3541 acgggtgcag gggcgccggt gcaggccggc tggcggcgtc gggggtaatg ggggacatgt
     3601 cggtcgagcc tcgtgcaatc ctctcatgat gggatcgtgg tgtcctggct accctgatgc
     3661 cggtcaaaat ccgcgatttg tcgatttccc ggccgccgat tcgtcgcttt cctgaaaccg
     3721 gcgcgttgcc ggtgctggag acaagtcatg cgcgtgatgg tgatcgtgaa ggcgagcccg
     3781 gagtcggaag gcggcggcat gccgaatacc gaggaactgg ctgccatggg caagttcaac
     3841 gaacaactgg tggaagccgg cgtgatgctg atgggcgaag gcctgcatcc gagttcgaaa
     3901 ggggcgcggg tgcgcttcga aggcaagcag cgcaccgtga tcgatgggcc tttttccgag
     3961 agcaaggagc tggtcgccgg cttctggttg tggcaggtgc gttcgatgga cgaggcgatc
     4021 gaatggatca agcgggcgcc gttcgacggc ggcatggaaa tcgaattgcg gccggtgttc
     4081 gagatggacg atttcggcga tgccgtcacg cccgaaatcc gcgagcggga agagcggttg
     4141 cgcgggaaga tcgccgaggc ctgaacgcgt ccgcatcggg cgcttgcgcc ggaggcgtgg
     4201 cgatggtgtg atcggccgcg atgaccacgg ccgatgccca tcgcaccatc gaagcggtct
     4261 ggaagatcga ggcgccgcgc ctgatcgcgg gactcgcgcg catgctgcgc gacgtcgggc
     4321 tggcggagga cctggcgcag gaagccctgg tcacggcgct ggaacgctgg cccggcgacg
     4381 gtgtccccga caaccccggc gcgtggctga tgaccacggc gaagcgccgc gcgatcgacc
     4441 agttgcgcca cgaccggatg cgcgacgaca agcaggccgc gatcgggcac gaactccaac
     4501 ctgacgccat ggcgcacgca ccggacaacg cggacgcgct ggatgacgac atcggcgacg
     4561 acctgctgcg gctgatcttc accgcctgtc atccggtgct cacccgcgac atgcaggtgg
     4621 cgttgaccct gcggatgctg ggcggcctca cgaccccgga aatcgcccgc gcgttcctgg
     4681 tgcccgaagc cacggtggcg cagcggatcg tgcgggccaa gcgcacgctt gcggaaaaga
     4741 aggttccgtt cgcggtgccg cgcggcgagg aactcgcggc gcgcctcgcg agcgtgctgg
     4801 aagtcgtgta cctcgtcttc aacgaaggct acgccgcgac ggccggtggc gactgggcgc
     4861 ggccggcatt gtgcgaggaa gccttgcggc tcggccgggt gctggcgggg ctcgcgccgc
     4921 gcgaacccga agtgcacggc ctcgtcgcgc tgatggaaat ccaggcctcc cgcctgcacg
     4981 cgcgcaccgc cgcggacggc agcccggtcc tgctgctgga gcaggaccgc gcccgctggg
     5041 atcgcttgct gatccggcgc ggcctggcat cgctggaacg cgcggagcaa ctcggcggcg
     5101 ccgatggcaa ctacgcgctg caggccgcga tcgcggcttg ccacgcccgg gccttgcacg
     5161 cggaagacac cgactggacg cggatcgcgt cgctgtacgc gcgcctcgcg gaagtcgcgc
     5221 cctcgccggt agtggaattg aaccgcgcag tcgcggtctc catggccgag ggcccggaag
     5281 ccgggctgcg gctggtcgac ggtctccggc aggagcgggt gctgcgcgac taccatctgc
     5341 ttcccagcgt acgcggcgac ctcctgttca agctgggacg cctggacgaa gcgcgggcgg
     5401 aattcgagcg ggcggccacg ctgaccggca acgaccgcga atcggaattg ttgcgccagc
     5461 gcgccgcagc gtgcggcgac ggaatgaccg ctgtccacct gccccttcaa ggggcaggcg
     5521 gggaggggga tgggccatga acaatcgaat cccgcatttc gtttggagcc cggacgcttc
     5581 cccaggcggg ttttcatgcg cagggcggac accgtccgcc acacttcgtg accggcgggc
     5641 aatgcccgcc ctgccgagcg gaccacgtag gttggtgccg agcgcagcga agcccaaccg
     5701 ggcatttcca gggagagatc gatggcctac atgttgttcg tagccgaacc gcgcggccag
     5761 cgcgccgagc gcaccgagga agagggcaag gcggtgtacc agcgcatgct ggattacgcc
     5821 gaggacctga aagcacgcgg cgtgcttcgc gcctactcgt ccttgcggaa cgattccgaa
     5881 ggccggcgcc tgcaagtccg cggcggcaag cgcagcctgc gtgacggccc cttcgccgaa
     5941 acgcgggaaa tgatcggtgg attcttcctg gtcgattgcg ccacccgcga ggaagccgtc
     6001 gagctcgccg cgctttgccc ggccgccgag tgggcgacca tggaagtgcg cgaaacgggg
     6061 ccgtgttatt tgcgctgacg gcggcaacac ttccacggga cgtgcgtcac gggccagcga
     6121 atcagtccct gtcccatggt tccgccggac cgcctttgtc gaagacgacg cgccagacgc
     6181 ccggtgcttc ccggcgccat atcgagttga aacggccgat caccttgccg tccggatcgc
     6241 ggaccagtcc ggtgctcaac gccagggtgc cggattgcag cacttccaca cggtccggct
     6301 cccacgagaa tggtgcggtc ttgccttcga agagccgcga ccacgccgca atcacttcct
     6361 gcttgccgcg cagtacgcca gtgtcagtga aaaagatggc ttccccggaa acgtgatggg
     6421 cgaacgcagg cagatctcga ttggccatgg acgcggcgaa cgcgcgttcc gcggcaagca
     6481 cctgttgctg ctgcgaatcg gatgacggca tggcaacgcg ccacggtgcg gccaacagca
     6541 tcgccaatgc gaggtgcgtt gcgatgcgtg acatgggact gcgacggcga aggccggtag
     6601 ggttcatggc gcattccgcg ggtggcggtg ggcggagcat acggcacgag ccagcgctgc
     6661 atcttgatgg atcgactggt cggcgggttt gcggacaaat caggacgagc tttgtggccg
     6721 cggtcgacac gcggagggcg ttggttacac ttcggcggca agtcgaagtc gtgcgttttc
     6781 aggcattcgc gaccgcggag gcgctcatgc attggcattg ccgactgtac ctgttcgtcc
     6841 tgctcggcct ggcggcatcg ccgccgcttg cggccgagcc tgcgtcagcg tcgtgcgatg
     6901 cgccggccta ccgccagttc gatttcttcg tgggtgactg ggatacctac gacgtgcagg
     6961 cacccggcag ggtcatcgcg cgcaacaccg tgaaagtcat
//
LOCUS       DASSNH010000073         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1685358, whole genome shotgun sequence.
ACCESSION   DASSNH010000073 DASSNH010000000
VERSION     DASSNH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685358"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFK00_02685"
     CDS             complement(<1..344)
                     /locus_tag="VFK00_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7591891.1"
                     /translation="MSRDYKQTINLPRTAFAMKADLARREPAMLADWESTRRYAELQD
                     RTGKRERAYILHDGPPYANGKIHLGHAVNKILKDIVVRSKLMAGFRSPYVPGWDCHGL
                     PIEIAVEKKYGKV"
     gene            complement(341..1297)
                     /locus_tag="VFK00_02690"
     CDS             complement(341..1297)
                     /locus_tag="VFK00_02690"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET7591892.1"
                     /translation="MLKVHRDIAGPCLAPGGCVLTIGAFDGLHRGHAALLAKVRERAT
                     KLGLVAAAVSFEPLPRSYFSPAPVKRLSSVREKLCGFVDAGIDQLLLLRFNAELVAMP
                     ADDFVREVLVARMGAREIWVGADFRFGHNRVGDVALLEAMQGEGPYEVRVLDAISTGD
                     DERVSASRVRGALGFGDFAHAAVLLGRPFTMSGRVVRGQGIGRDLGYPTANIRLGTRV
                     SPVSGIFAVRVRVGDRWWPGVASLGVRPTVAGGGEPLLEAHLFDFDGDLYGQRIEVEF
                     VAKLRDEEKFADLDALRAQMDRDAAEARRILADISTVSGAFA"
     gene            complement(1449..1940)
                     /gene="folK"
                     /locus_tag="VFK00_02695"
     CDS             complement(1449..1940)
                     /gene="folK"
                     /locus_tag="VFK00_02695"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7591893.1"
                     /translation="MPGPFAAYVALGSNLDDPEAQVEGGFAALAALPGTRLRARSRLY
                     RTPPWGVVDQPAFVNAAAALETSLAPRALLEALLAIEAGAGRVRGVRNGPRTLDLDLL
                     AYGDCELREAGLTLPHPRLHERAFVLLPLADVAPGFDVPGQGRVADLLANVDTTGCVA
                     IDG"
     gene            complement(1944..>2129)
                     /locus_tag="VFK00_02700"
     CDS             complement(1944..>2129)
                     /locus_tag="VFK00_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591894.1"
                     /translation="LRESGEWWTRAQELSADELSASLGPKPAAGHAGEPAAKPKRRRR
                     RGGRRRPAGEARPTSQD"
BASE COUNT          329 a          783 c          706 g          311 t
ORIGIN      
        1 accttgccgt acttcttctc gaccgcgatc tcgatcggca acccgtggca atcccagccc
       61 ggcacgtacg gcgagcggaa gcccgccatc agcttcgagc gcaccacgat gtccttcagg
      121 atcttgttga ccgcgtgccc caggtggatc ttgccattcg cataaggggg gccgtcgtgc
      181 aggatgtacg cgcgctcgcg cttgccggtg cggtcctgca gttcggcgta acgccgcgtg
      241 ctttcccaat ccgccagcat cgccggctcg cgcctggcga gatcggcctt catcgcgaag
      301 gcggtgcgtg gaagattgat ggtctgcttg tagtcgcggc tcacgcgaac gctccggaaa
      361 cggtggatat gtcggcaagg atgcgccgcg cttcggcggc atcgcgatcc atctgcgcac
      421 gcagcgcgtc caggtcggcg aatttttcct cgtcgcgcag cttggcgacg aactcgactt
      481 cgatgcgctg gccgtagagg tcgccatcga agtcgaacag gtgtgcttcc agcagcggct
      541 cgcccccgcc cgcgaccgtc gggcgcacgc cgaggctcgc gacgcccggc caccagcggt
      601 cgccgacgcg cacgcgcacc gcgaagatcc cggacaccgg cgacacccgc gtgcccagcc
      661 ggatgttggc ggtcggataa ccgaggtcac ggccgatgcc ctggccgcgc accacccggc
      721 cgctcatcgt gaaggggcgc cccagcaaca ccgccgcgtg cgcgaaatcc ccgaagccca
      781 gcgccccgcg cacccgcgag gccgacacgc gctcgtcgtc gccggtcgag atcgcgtcca
      841 gcacgcgcac ctcgtaggga ccttcgcctt gcatggcttc gagcagcgcc acgtcgccca
      901 cgcggttgtg gccgaagcgg aaatcggcgc cgacccagat ttctcgcgcc cccatccgcg
      961 cgaccagcac ctcgcgcacg aaatcgtccg cgggcatcgc caccagttcg gcgttgaagc
     1021 gcaacagcag caattggtcg atgccggcat cgacgaaccc gcacagcttt tcgcggaccg
     1081 aggacaggcg cttgaccggc gcgggcgaaa aatagctgcg cggcagcggt tcgaaactca
     1141 ccgccgcggc gacgaggccg agcttcgtcg cgcgttcgcg caccttcgcc agcaacgcgg
     1201 cgtgtccgcg gtgcagtccg tcgaacgcgc cgatcgtaag cacgcagccg ccgggggcca
     1261 ggcatggccc tgcgatgtca cgatgaacct tgagcatcga aaaagtatat tggagcgggg
     1321 tttgctcgtg cgatcgtccc tgatcgcgcg acttccggat gtgctcgccg cgaagccgct
     1381 ccgcgtcgcc gacacgccaa cggcgctcca cgacccggct tttttcacac cacccctccg
     1441 atcgaagctc acccgtcgat ggcgacgcag ccagtcgtgt cgacgttggc cagcagatcg
     1501 gcaacgcggc cctgccccgg cacgtcgaac ccgggcgcga cgtcggccaa cggcaacagc
     1561 acgaaggccc gctcgtgcag ccgcgggtgc ggcagggtca gtcccgcttc gcgcaactcg
     1621 cagtcgccgt acgccagcag gtccaggtcg agcgttcggg ggccgttgcg gacgccacgt
     1681 acccgtcccg cgccggcctc gatcgcgagt agcgcctcga gcagcgcgcg gggcgcgagc
     1741 gaggtttcga gcgccgccgc cgcattgacg aaggcgggct ggtcgacgac gccccacggc
     1801 ggcgtgcgat acaggcgcga gcgggcgcgc agccgcgtgc ctggcaacgc cgccagcgcg
     1861 gcgaaaccgc cttcgacctg cgcttcggga tcgtccaggt tgctgcccag cgccacgtac
     1921 gcggcaaacg gccccggcat gcttcagtcc tgcgaggtcg gccgcgcttc ccccgcgggg
     1981 cggcgccggc cgccgcgccg ccgccggcgt ttcggcttgg cggccggttc gccggcatga
     2041 cccgcggccg gcttggggcc gagcgacgcg gaaagttcgt cggccgaaag ttcctgcgcc
     2101 cgggtccacc attcgcccga ttcgcgcag
//
LOCUS       DASSNH010000074         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_831339, whole genome shotgun sequence.
ACCESSION   DASSNH010000074 DASSNH010000000
VERSION     DASSNH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831339"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFK00_02705"
     CDS             complement(<1..501)
                     /locus_tag="VFK00_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591895.1"
                     /translation="MAFPDFFAEVPAIRLHDGLAQLLGASDDGMIEYHYADAVRLAGH
                     SCPTVAGGWLSARAALRHLYPDSMPERGGISVYLDDAEDAGVTGVIGQVLTLVTGAAV
                     ANGFKGLGGRYARNHLLHYAQGDVAGVRLRRNDTGDEVEIEIDASSIPPDPMQRLLIQ
                     LVLSGQA"
     gene            complement(501..761)
                     /locus_tag="VFK00_02710"
     CDS             complement(501..761)
                     /locus_tag="VFK00_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HET7591896.1"
                     /translation="MNATADTPTYQAYPFDARAIAHRFRHSAIFGALDALEPGEAMRF
                     VNDHDPIPLLRQMQQRYGDHIAVIYRQRGGEGPVVIDFVVAQ"
     gene            complement(831..1916)
                     /locus_tag="VFK00_02715"
     CDS             complement(831..1916)
                     /locus_tag="VFK00_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7591897.1"
                     /translation="MSVNARRQRYKTIARALSAAAAATALAGCSTLGYYAHLAAGEMA
                     VLRARQPIPDVIADPDTPPALRQRLHLALRARAFASDVLKLPRNRSYTTYADIHRPYV
                     MWNVFATPALSLLPIEHCFLFAGCVAYQGFYRHDRAEALADRLAKGGDDVWIGGVPAY
                     STLGHFADPLLSTMDRWSDDEMVGTIFHELAHQEVYVKGDTAFNESFATFVQREGLRQ
                     WHADNHLPPPDPAIADRQRQFTELVLATREKLQALYTSGASDAEKRAGKAQAFADLRR
                     NYAHLRDNEWHGYQGYDHWFDAPINNAKLAPFGLYDRGVPAFAALFERCGGNWTRFYA
                     AVRKLGGDPAKQRDAFLKSASATSAGN"
     gene            2071..>2345
                     /locus_tag="VFK00_02720"
     CDS             2071..>2345
                     /locus_tag="VFK00_02720"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295487.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HET7591898.1"
                     /translation="MLLLIDNYDSFTFNLAQYLGELGQDVKVLRNDAVDVAGIRALAP
                     ERIVVSPGPGRPEHAGVTLALIREFAGSIPILGVCLGHQAIGQAFGG"
BASE COUNT          375 a          760 c          821 g          389 t
ORIGIN      
        1 ggcctgcccg gaaagcacga gctggatcag caggcgctgc attggatcgg gcggtatcga
       61 gctcgcatcg atttcgattt cgacttcatc gccggtgtcg ttgcggcgta aacgcacgcc
      121 tgcgacgtcg ccttgcgcgt agtgcagcag gtggttgcgt gcgtaccggc cgcccaaccc
      181 cttgaagccg ttggcgaccg cggcaccggt gacgagcgtc agcacctggc ctatcacgcc
      241 ggtgacgccg gcgtcttcgg cgtcgtccag gtacacgctg atgccgccgc gctcgggcat
      301 gctgtcggga tacaggtgac gcagcgcggc gcgcgcgctg agccacccgc cggcgacggt
      361 ggggcaggag tgcccggcca gcctcaccgc gtcggcgtag tggtactcga tcatgccgtc
      421 gtcactggcc cccagcagtt gcgcgagccc gtcgtgcaac cggattgccg gcacttcggc
      481 gaagaaatcc gggaacgcca ttactgcgcc accacgaaat cgatgactac gggaccctca
      541 ccgccgcgct gccggtagat caccgcgatg tggtcgccgt agcgctgctg catctggcgt
      601 agcagcggga tcggatcgtg gtcgttgacg aaacgcatcg cctcgccggg ctccagcgca
      661 tccagcgcgc cgaagatggc cgaatgtcgg aaacggtgcg caatggcgcg ggcgtcgaat
      721 ggataggctt ggtaggtcgg cgtgtcggca gtcgcgttca tgcggagtcc gggagcggag
      781 gaagccgcgc caagcatgcg ccgcgcgggc gccggaaatc ttgacctgcg tcagttgccg
      841 gctgacgtgg cggaggcgct tttgaggaac gcgtcgcgct gcttcgccgg gtcgccgccg
      901 agcttccgca cggcggcgta gaagcgcgtc cagttcccgc cgcaccgctc gaacagggcg
      961 gcaaacgcgg gcacgccccg gtcgtagagg ccaaacggcg ccagcttggc gttgttgatg
     1021 ggcgcgtcga accaatgatc gtagccctga taaccatgcc attcgttgtc gcgcaggtgc
     1081 gcatagttgc gacgcaggtc agcgaacgcc tgcgccttgc cggcacgctt ttccgcatcg
     1141 gacgcgccgc tcgtgtacag ggcctgcagc ttctcgcgcg tggcgagcac cagttcggtg
     1201 aactgccttt ggcggtcggc gatggcggga tccggcggcg gcaggtggtt gtcggcgtgc
     1261 cactgccgaa ggccctcgcg ctgcacgaag gtcgcgaacg actcgttgaa ggcggtgtcg
     1321 cccttgacgt agacctcctg gtgcgcgagc tcgtggaaga tcgtcccgac catctcgtcg
     1381 tcgctccacc ggtccatcgt gctcagcagc gggtcggcga aatggccgag cgtcgaatat
     1441 gccggcaccc cgccaatcca tacgtcgtcg ccgcccttgg ccaggcggtc cgccagtgcc
     1501 tcggcgcggt cgtggcggta gaagccctgg taggcgacgc agccggcgaa caggaaacag
     1561 tgctcgatcg gcagcagcga caacgccggc gtcgcgaaca cgttccacat cacgtacggc
     1621 cggtggatgt cggcgtaggt cgtgtaactc cggttgcgcg gcagcttcaa cacgtcggag
     1681 gcgaacgcgc gggcacgcag cgcgaggtgc aaccgctggc gcagcgcggg aggcgtgtcc
     1741 ggatcggcga tcacgtccgg gatcggctgg cgggcacgca gcaccgccat ctccccggca
     1801 gcgagatggg cgtagtagcc gagggtcgaa cagccggcga gcgcggtggc tgccgcggcc
     1861 gccgacagcg cgcgcgcgat cgtcttgtat cgttgtcggc gcgcgttcac actcacgttg
     1921 ttcggaggat cgggacgagg tttgtggaag ttcattgcgt gaacacgcag catcatgctc
     1981 ccacaccccc cacccttgct tcgcaagggt cgcccccttg actcaagggg gccgggttcc
     2041 tctacgtcga agttgaacgg accggaagac atgctgctgc tcatcgacaa ctatgacagc
     2101 ttcacgttca acctcgcaca gtacctgggc gaactcgggc aggacgtgaa ggtgctgcgc
     2161 aatgacgccg tggacgtcgc cggtatccgg gcgctcgcgc ccgagcggat cgtggtgtcg
     2221 ccgggcccgg gcaggccgga acatgccggg gtgaccctgg cgctgatccg cgaattcgcg
     2281 ggctcgattc cgatcctcgg cgtgtgcctc ggccaccagg cgatcggaca ggcgttcggc
     2341 ggcaa
//
LOCUS       DASSNH010000075         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1684485, whole genome shotgun sequence.
ACCESSION   DASSNH010000075 DASSNH010000000
VERSION     DASSNH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684485"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /gene="glgX"
                     /locus_tag="VFK00_02725"
     CDS             complement(<1..1245)
                     /gene="glgX"
                     /locus_tag="VFK00_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7591899.1"
                     /translation="MPNNNQPRVLPGAPYPLGASWDGRGVNFALFSANAEKVELCLFD
                     RVDRPESARVVLPDRSGNVWHGYLPEARPGTLYGYRVYGPYDPAHGHRFNHHKLLLDP
                     YARLIRGRLRWSDAHFGYRVGSPREDLSFDRRDNGRTMPKCQVVDPAFSWGVHRPPTH
                     SWSDTVIYETHLRGYTMRHPALPNGLRGTVAGFGSAPVIDYLKSLGITAVELLPVQAY
                     VHDRHLVDKGLTNYWGYNTIGFFAPEPGYLSTGQLHEFKTMVARLHDAGIEVILDVVY
                     NHTGEGNQLGPTLCFRGIDNASYYRLQDDPRYYCDDTGTGNSLDLTHPRVLQMVMDSL
                     RYWAGEMQVDGFRFDLATTLARGPNGFDPHCSFLDAVAQDPLLARTKLIAEPWDVGPG
                     GYQVGRFGANWSEWNDRYRDSVR"
     gene            complement(1483..3684)
                     /gene="glgB"
                     /locus_tag="VFK00_02730"
     CDS             complement(1483..3684)
                     /gene="glgB"
                     /locus_tag="VFK00_02730"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET7591900.1"
                     /translation="MTPEQQQPLGLGAAQIESIVSGNCGDPFASLGPHPLSDGRTVVR
                     VFMPDTSAVAVVGPRGGKLAECTRIHSEGLWVGTLRRPGRYRLRAQTRDGAVEFEDAY
                     RFGEVLGEMDVYLLAEGTHLRNYQRLGAHPREMDGVAGTAFAVWAPSARRVSVVGDFC
                     NWDGRRLPMRKRHECGVWELFVPGVGAGALYKFEIKGADGTLLPLKADPYALRAEHPP
                     RTASIVHVLHDAEPHDDAWMARREAAQATAAPISIYEVQLGSWRRNEGNRYLSYGELA
                     DQLIPYARDLGFTHLELMPVSEYPFDGSWGYQPVGMYAPTSRFGTPDDFVRFVERCHD
                     AGLGVLLDWVPGHFPTDPHGLGWFDGTHLYEHADPRQGFHQDWNTLIFNYGRREVRNY
                     LLGNALFWLQQYHADGLRVDAVASMLYLDYSRKAGEWVPNRSGGRENLEAIDFLKAMN
                     EHAFGDCPGATTIAEESTAWPGVSRPTYDGGLGFGYKWNMGWMHDTLTYMQEDPVHRG
                     WHHDKLTFGLVYAFTENFILPLSHDEVVHGKGSLLGKMPGDRWQRFANLRAYFGFMWG
                     HPGKKLLFMGGEFAQEGEWNHDRSLDWHLLAQPEHRGMQNLVRDLNHAYRELPALHRR
                     DCEATGFEWVEMNDRAQSVLAFLRLGAEGDRPALVVCNFTPVPRHGYRVGVPCGGFWR
                     ERINTDAADYGGSGTGNLGGVHGEEIAWHGRSHSLVVTLPPLATVIFESAG"
     gene            complement(4101..>4304)
                     /locus_tag="VFK00_02735"
     CDS             complement(4101..>4304)
                     /locus_tag="VFK00_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591901.1"
                     /translation="ENIAGVESYPADPATARHWLDLFTLEKACYEIVYEANNRPNWLL
                     IPVRGVRQLMDGHATASTAGPPP"
BASE COUNT          691 a         1376 c         1480 g          757 t
ORIGIN      
        1 gcgcacgctg tcgcggtagc gatcgttcca ttcggaccag ttggcgccga agcggccgac
       61 ctggtagccg cccggtccga cgtcccaggg ttccgcgatc agcttggtcc gtgcgagcaa
      121 cggatcctgt gcgaccgcgt cgaggaaact gcaatgcgga tcgaacccgt ttggcccgcg
      181 tgcgagcgtg gtcgcgaggt cgaaacggaa gccgtccacc tgcatttcgc cggcccagta
      241 gcgcagcgag tccatcacca tctgcaggac gcgggggtgc gtcaggtcga ggctgttgcc
      301 ggtgccggta tcgtcgcagt agtagcgggg atcgtcttgg agccggtaat agctggcgtt
      361 gtcgatgccg cggaaacaca aggtcggccc gagttggttg ccctcgccgg tatggttgta
      421 gacgacgtcc aggatcactt cgatgcccgc gtcgtgcagc ctcgccacca tcgtcttgaa
      481 ctcgtggagc tggccggtgg acagataccc gggttccggc gcgaagaagc cgatggtgtt
      541 gtagccccag tagttggtga gccccttgtc gacgaggtgg cggtcgtgga catacgcctg
      601 taccggcagc agctcgacgg cggtgatccc gaggctcttc aggtagtcga tgacgggtgc
      661 gctgccgaag cccgcgacgg tgccgcgaag tccgttcggc aatgcggggt gccgcatcgt
      721 gtagccgcgc aggtgcgttt cgtagatgac cgtgtcgctc caggagtgcg tgggcggccg
      781 gtgcacgccc caactgaacg cggggtccac gacctggcat ttcggcatcg tgcggccgtt
      841 gtcgcggcgg tcgaacgaga ggtcctcgcg cggcgagccg acccggtagc cgaaatgcgc
      901 atcggaccag cgcagccggc cgcggatcag cctggcatac gggtcgagca gcaacttgtg
      961 gtggttgaag cggtggccgt gggcgggatc gtagggaccg tacacgcggt agccgtacag
     1021 cgtccccggc cgcgcttcgg gcaggtatcc gtgccagacg ttcccactcc ggtccggcag
     1081 cacgacgcgc gcgctttccg ggcggtccac gcgatcgaac aggcacagct cgaccttctc
     1141 ggcattcgcg gaaaacagcg cgaaattgac accgcgaccg tcccagctcg cgccgagcgg
     1201 atagggagcg cccggcagga cgcgaggttg attgttgttg ggcatgatca aggatagctg
     1261 cgacgcaata gcaaaccctc tcccccgagc agctctatcg cttgggggag agggcagggt
     1321 gagggggcga ggcggtcagg tggcgatgcc cctcacccca gccctgcccc aaaaggtgaa
     1381 tccatttggg ggaggggagc gattcattcc gccgtgatcc acgttcgtcc gcgttggatt
     1441 gtgacctgct ttcattcgcg cgggtctgca agaattctca tttcagccgg cggactcgaa
     1501 gatcacggtc gccagcgggg gcagggtcac gaccagcgaa tggctccggc cgtgccatgc
     1561 aatttcttcg ccatggacgc cgcccaagtt gcccgtgcca cttccgccat agtcggccgc
     1621 gtcggtgttg atgcgttcgc gccagaaacc gccgcacgga acaccgacac gatacccgtg
     1681 gcggggcacc ggtgtgaagt tgcacacgac gagggcaggg cggtcgccct cggcgccgag
     1741 gcgcaggaac gccagcacgc tctgcgcgcg gtcgttcatc tcgacccatt cgaaaccggt
     1801 cgcttcgcaa tcgcgccggt gcagcgccgg cagttcgcgg tacgcgtggt tcaggtcgcg
     1861 cacaaggttc tgcatgccgc gatgctccgg ctgcgccagc aggtgccagt cgaggctgcg
     1921 gtcgtggttc cactcgcctt cctgggcgaa ttcgccgccc atgaacagca attttttccc
     1981 gggatggccc cacatgaatc caaagtaggc gcgcaggttc gcgaagcgct gccagcggtc
     2041 gcccggcatc ttgccaagca gcgacccctt gccgtgcacc acttcgtcgt gcgacagcgg
     2101 caggatgaag ttttccgtga acgcgtagac cagcccgaac gtcagcttgt cgtggtgcca
     2161 gccgcggtgt accgggtcct cttgcatgta ggtgagggtg tcgtgcatcc agcccatgtt
     2221 ccatttgtag ccgaagccga gcccgccgtc gtaggtcggc cgcgagacac ccggccacgc
     2281 cgtcgattcc tcggcgatgg tggtcgcgcc gggacagtcg ccgaacgcgt gctcgttcat
     2341 ggctttcagg aaatcgatcg cttcgaggtt ctcgcgcccg ccggagcgat tgggtaccca
     2401 ttcgccggcc ttgcggctgt agtcgaggta gagcatcgat gcgaccgcgt ccacccgcag
     2461 gccgtcggcg tggtattgct gcagccagaa cagggcgttg ccgagcaggt agttgcgcac
     2521 ctcgcggcgg ccgtagttga agatcagcgt gttccagtcc tgatggaacc cctggcgcgg
     2581 atcggcgtgc tcgtagaggt gggtgccgtc gaaccagcca aggccgtgcg gatccgtcgg
     2641 gaagtggccg ggcacccagt ccagcagcac gccgagtccg gcatcgtggc atcgctcgac
     2701 gaagcgcacg aagtcatcgg gcgtgccgaa gcggctggtc ggcgcgtaca tcccgaccgg
     2761 ctggtagccc cacgagccgt cgaacgggta ttcgctgacc ggcatcagtt cgaggtgggt
     2821 gaagccgagg tccctggcgt acggaatcag ttggtccgcc agctcgccgt acgagagata
     2881 gcggttgcct tcgttgcgcc gccacgagcc gagctggacc tcgtagatgg aaatgggcgc
     2941 ggcggttgcc tgcgcggctt cgcgccgtgc catccaggca tcgtcgtgcg gctccgcgtc
     3001 gtgcaggacg tgcacgatcg acgcggtgcg cggcggatgt tccgcccgca gtgcgtaggg
     3061 atcggccttg agcgggagca gcgtgccgtc cgcgcccttg atctcgaact tgtagagcgc
     3121 gcccgcgccg acgccgggca cgaacagttc ccatacaccg cactcgtggc gcttgcgcat
     3181 cggcaggcgc cggccgtccc agttgcagaa gtcgccgacc acgctgacgc ggcgcgcgct
     3241 gggcgcccac accgcgaacg cggtacccgc tacgccatcc atctccctcg ggtgcgcgcc
     3301 gaggcgctgg tagttgcgca ggtgcgtgcc ctcggccagg aggtacacgt ccatttcgcc
     3361 gagcacttcg ccgaaccggt aggcatcctc gaattcgacc gcgccgtcgc gggtttgcgc
     3421 gcgcagccgg tagcgtcccg gacgccgcag cgtacccacc cacaatccct ccgaatggat
     3481 gcgcgtgcac tcggcaagct tgcctccgcg cggcccgacc accgccacgg ccgatgtatc
     3541 cggcatgaac acccgcacca ctgtcctgcc atcggaaagc gggtgcgggc cgagcgaggc
     3601 gaagggatcc ccgcaattgc cgctgacgat ggattcgatc tgggcggcgc cgaggccgag
     3661 cggttgttgt tgctcaggag tcatttcgac aaatcaagtc caatggtttt tcgcttctcc
     3721 cctcgggaga aagtggcgcg cagcgccgga tgagggtgag gggcggcgtt tgcgtgatgc
     3781 cggtggcatc gcgcgacgct ccgaacgggc ggccaaggat ggccgcaccg gcgcccacac
     3841 cagggacggt tgcttgcgtg cttcatacgc atcttgcgct gcgcgcgcct ttcccgacgg
     3901 gagaggaact cttttccctt ctccgctcgg gagaaggtgg cgcgcagcgc cgggtgaggg
     3961 tacggacaca cgactggccg gttgccgcga ggatgttgcg gtggagcgct ggcgcaccct
     4021 gacccgcccc ttcggggcag cctctcccga ggggagagga attctttcgc gttggagaag
     4081 aaccgtttca gccaggaaag tcatggtggt ggcccagccg tgcttgccgt cgcatgtccg
     4141 tccatcagtt gccgcacgcc gcgaaccggg atcagcagcc agttcggccg gttgttggcc
     4201 tcgtacacga tctcgtagca ggccttctcc agcgtgaaca ggtcgagcca gtggcgtgcg
     4261 gtcgcgggat cggccggata gctttcgacg ccggcgatgt tctc
//
LOCUS       DASSNH010000076         7735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_570254, whole genome shotgun sequence.
ACCESSION   DASSNH010000076 DASSNH010000000
VERSION     DASSNH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570254"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /gene="serC"
                     /locus_tag="VFK00_02740"
     CDS             complement(<1..646)
                     /gene="serC"
                     /locus_tag="VFK00_02740"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET7591902.1"
                     /translation="MSRGWNFSAGPAAMPEEVLGRAQRELVEWGGARASVMEVSHRGK
                     HFMDMAASAEADLRELLAVPGNYKVLFLPGGATQQFAQIPMNIAADGQRADYVVTGHW
                     GEKAVGEAAPYARAHVAASSKADGYTSIPERATWDLDPGAAYVHITTNETIHGVEMRD
                     IPDVGAVPLAADMSSDILSHPLDVSKFGLVYAGAQKNIGPSGLVVMIAREDLLER"
     gene            complement(683..1435)
                     /locus_tag="VFK00_02745"
     CDS             complement(683..1435)
                     /locus_tag="VFK00_02745"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7591903.1"
                     /translation="MRIVCFHPARDPVMCGEGPVRVGSALDDTVVLDGAGVEPHHLTI
                     VADARGLVLKVRAGCQRVYVNARAVREQALLHCGDTITLGANKFLVTSNGPPAPADAA
                     CDPGAAMGDVVLRVVSGAGSGQALPVASELQLGAGSRHFGELPYTCRVAQAGGRLVFE
                     SRSNLPRINGWRRNHVALAPNDQIVLGEHRLLVEAPGLQYARHVTSLPPPLQQVQETP
                     ERDEPPPTEIWWLIGAAAVLAAIIAMFLYFRW"
     gene            1640..1858
                     /gene="infA"
                     /locus_tag="VFK00_02750"
     CDS             1640..1858
                     /gene="infA"
                     /locus_tag="VFK00_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7591904.1"
                     /translation="MAKDDVIEMEGTVQETLPNTMFRVQLENGHVVTAHISGRMRKHY
                     IRILTGDKVKVEMTPYDLTKGRITYRMR"
     gene            complement(1873..4626)
                     /locus_tag="VFK00_02755"
     CDS             complement(1873..4626)
                     /locus_tag="VFK00_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7591905.1"
                     /translation="MRRGLFPAVHSPLTKRLLPLAYALGAMVAFILLLTWAAFQIQIT
                     LAGFLNGESVWSKAQKQSAVDLLNYATTGDPAAYRDFERNFVVLDTYRTARDKIQSGY
                     FDYDQVEEELRRTKAMPVAIPGVIFMLQHFGNAPYMKDAVRVWRSTDDSIAQLHAIAG
                     ELHQAYASGPIPRDEIIRQRRRINVLNDYIQPRSNDFSLFVAHGAVSIGRALYVGVLT
                     SAAVAFMLWLLLARRTLARIRGSEERYRLLFDSAPDAIVMVDERSGRILDANRTAGAW
                     MGREPQALMGGSYADLFETSALRDGLPGDGELRDAHGRTRPIETQSSMAVWGEQLVRQ
                     AIIRDITERVESDRERRIAAEALASIAEGVFIADATRRIVSANAAATQITGFTPEHLI
                     GMRLDDTRRMRDGGPLPATIWDEIIATHHWTGEVESRRRDGTTFTEKLSISTIRDAEH
                     RVLHYVAVFSDISAAKEDEYRLQHLAAHDPLTGLVNRAEFQRRCDVAIELAARDRRSA
                     VVLFIDLDAFKFVNDSYSHAVGDSLLKLVADRIRHQLSGDAITGRIGGDEFTVLLPRL
                     TLREDAAALADRLVSALSEPFNVEGYEIVLGASIGIAGFPLDGSDAPTLIANADAAMY
                     AAKIEERNAWRFYMPTMQADSRHRLMLATELRQALQKEEFRLLYQPSVELQTGRIVAA
                     EALLRWQHPERGEVAPGEFIPIAESIGMIHRIDEWVMQAVCRQIRTWDAAGVPPIRVA
                     INVSARWFGHPGFIESIRRALQANGVPPQRIALEITEGAMLRLGEETEHTMRALHALG
                     IGVAIDDFGTGYSSMAYLKLPAVAYLKIDRSFVSGLPGNANDAAIATAMVAMAGSLGL
                     VTIAEGVETEAQHEFLARAGSTEGQGYLYSYPLPPSAVERMLRPKGQNVGAKLKLVPP
                     CRG"
     gene            complement(4701..5480)
                     /locus_tag="VFK00_02760"
     CDS             complement(4701..5480)
                     /locus_tag="VFK00_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET7591906.1"
                     /translation="MNVRFERSGPLTELGSYPEWAQDLVGRCDVVKKTVVDHELWDVM
                     RDEPTRQCTANFMVGIWPVIERFPGYMAHSLLKTSYGRSTGDDLARRWLVRNIRVEQN
                     HAEYWLMWAEGAGVPRKQVLSGVVPHGTQVLANWCEEISSRGSLAASIAATNYAVEGA
                     TGEWSQILYDSESYAEQFPAATRKASLRWLQLHAAYDDTHPWEALEIVCTLMGTSPAA
                     ADVAHIEECVKRSYVSMRMLADRCVQYTGEMEAGESYRAVA"
     gene            complement(6071..6991)
                     /locus_tag="VFK00_02765"
     CDS             complement(6071..6991)
                     /locus_tag="VFK00_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HET7591907.1"
                     /translation="MAQPYPIGTPGKPWGEAEKAEWRSRQHKQRSYADDVLAAIERLR
                     SRFDVEQYGKLDYEPDAYPLFALKTHGWRDASPIMLVTGGVHGYETSGVHGALEFAER
                     HAADYAGRANILVVPCVSPWGYERIHRWNPYALDPNRNFRADSPAPESAALIRFLMPY
                     RDRVWMHIDLHETTDTDESEFRPALAARDGVAYEPGTVPDGFYLCADSANPQPGFQQA
                     VIAAVAKVTHIAPADDKGEIIGSPVVAPGVIEYALERLGLCAGVTAARYRTTTEVYPD
                     STRATPEQCNQAQIAAACAAIDFALTHPEA"
     gene            complement(7178..>7735)
                     /locus_tag="VFK00_02770"
     CDS             complement(7178..>7735)
                     /locus_tag="VFK00_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET7591908.1"
                     /translation="ATAGTVVQECGAGVIDIRHRDGRLAFAAPPLRRDGPVAADLADR
                     IVAGLRVAPEKVRAMQWVDNGPGWIGVLLDSARSALELEIDKATLAGLDVGVIAPHPA
                     GGPADFEVRAFFTGDGMLEDPVTGSLNASLAQWLIGDGTAPPRYRARQGTVLGRSGIV
                     DIEQEGDRIWVGGACITCIEGSVRL"
BASE COUNT         1254 a         2601 c         2495 g         1385 t
ORIGIN      
        1 ggcgctccag caggtcctcg cgcgcgatca tcaccaccag gcccgagggc ccgatgttct
       61 tctgtgcgcc cgcgtagacc aacccgaact tcgacacatc caacggatgc gacaggatgt
      121 ccgaggacat gtccgccgcc agcggcaccg cgccgacgtc ggggatgtcg cgcatctcga
      181 cgccgtggat ggtttcgttg gtggtgatgt ggacgtaggc ggcaccggga tcgaggtccc
      241 atgtcgcgcg ctccggtatg gacgtatagc cgtccgcctt cgaactggct gcgacatgcg
      301 ccctggcgta cggggccgct tcgccgacgg ccttttcgcc ccagtgcccg gtcaccacgt
      361 agtccgcgcg ctgaccgtcg gccgcgatgt tcatggggat ctgcgcgaac tgctgggtcg
      421 cgccgccggg caggaacaac accttgtagt tccctggaac ggcgagcaac tcgcggaggt
      481 ccgcctcggc gctggcggcc atgtccatga agtgcttgcc gcgatggctc acttccatca
      541 ccgaggcgcg tgccccgccc cattccacga gctcccgttg cgcgcgcccc aggacttctt
      601 cgggcatcgc ggccggcccg gcgctgaaat tccatcctcg tgacattgcc agacgttcct
      661 cgctcatcac atccgcaaaa cgctaccagc gaaaatacag aaacatcgcg atgatcgccg
      721 ccagcaccgc tgcggcgccg atcagccacc agatttcagt cggcggcggc tcgtcgcgct
      781 ccggggtttc ctgcacttgt tgcaggggtg gcggaaggga cgtcacatgt cgcgcatact
      841 ggagtcccgg cgcttcgacc agcagccgat gctcgcccag cacgatctgg tcgttgggtg
      901 ccagcgcgac atggttgcga cgccagccat tgatgcgcgg caggttgctg cgcgactcga
      961 agaccagcct gccccccgcc tgcgcgacgc gacaggtata cggcagttca ccgaagtggc
     1021 ggctgccggc gccgagctgc agttccgacg cgacgggcag cgcctgcccg gagccggcgc
     1081 cggaaacgac gcgcaggacg acatcgccca tcgctgcgcc aggatcgcag gccgcatcgg
     1141 ccggcgccgg cggaccattc gaggtcacca ggaacttgtt ggcgcccagc gtgatggtgt
     1201 cgccgcaatg cagcagggcc tgttcccgca ccgcgcgcgc attcacgtag acccgctggc
     1261 acccggcccg gaccttcaac accagcccgc gtgcatcggc gacgatcgtg aggtggtgcg
     1321 gttcgacgcc cgcaccgtcg agcaccacgg tatcgtcgag agcgctgccg acgcgcaccg
     1381 gaccttcccc gcacatcacg gggtcgcggg ctggatggaa gcagacgatg cgcacgccgg
     1441 gtaccttgcc gaatcgcgcg caatgtagca tgccgcaccg cagcaagagc cccggatccg
     1501 tgcgcttcgg atgctgcagc gtgcagcgct ccgctttgct cgaccatgcc cgtaccgggg
     1561 ctcgctgccg gtcgcgcttt cgcgcgtcgc cgcatcgcgt tacaatggaa agctattcgc
     1621 ctcgcgcatt gcgccaccca tggccaaaga cgacgtcatc gaaatggaag ggacggtgca
     1681 ggaaaccctg cccaacacca tgttccgggt ccaattggaa aatggccacg ttgtcaccgc
     1741 gcacatatcc ggcaggatgc gcaagcacta catccggatc ctcaccggcg acaaggtcaa
     1801 ggtggaaatg acgccctacg acctcaccaa ggggcgcatc acctaccgca tgcgctgacg
     1861 cgcggtcgcc gctcaaccgc ggcagggtgg caccagtttc aacttcgcac cgacgttctg
     1921 ccccttcggg cgcaacatgc gctcgaccgc ggacggcggg agcgggtacg aatagagata
     1981 gccctgcccc tcggtgctgc cggcgcgcgc caggaattcg tgctgggctt cggtctcgac
     2041 cccttcggcg atcgtgacga gtccgaggct gccggccatc gccaccatgg ccgtggcgat
     2101 cgccgcgtcg ttggcattgc cgggcagtcc gctgacgaag gaccggtcga tcttgaggta
     2161 cgcgaccgcg ggcagcttga gatacgccat cgacgaatag ccggtgccga aatcgtcgat
     2221 ggccacgccg atgccgagcg cgtgcagggc gcgcatcgtg tgctcggttt cctcgcccag
     2281 ccgcaacatg gcgccctcgg tgatttccag cgcgatccgc tggggtggaa caccgttcgc
     2341 ctggagggcc cgccggatgc tttcgatgaa gccggggtgg ccgaaccagc gggccgacac
     2401 gttgatcgcg acccggatcg gcggcacgcc cgccgcgtcc caggtgcgga tctgccggca
     2461 caccgcctgc atgacccact cgtcgatccg gtggatcatg ccgatgctct cggcgatcgg
     2521 gatgaattcc ccgggcgcca cctcgccgcg ctcggggtgc tgccagcgca gcagcgcctc
     2581 ggccgccacg atccgcccgg tctgcaattc cacgctcggc tggtacagca gccggaattc
     2641 ctctttctgc agcgcctggc ggagttcggt cgcgagcatc agccggtggc gcgagtccgc
     2701 ctgcatcgtc ggcatgtaga agcgccaggc gttgcgctcc tcgatcttgg ctgcgtacat
     2761 cgccgcgtcc gcgttggcga tcagggtcgg cgcgtcgctg ccatccagcg ggaatcccgc
     2821 gatgccgatg ctggcgccga gcacgatttc gtagccttcc acgttgaacg gttcggaaag
     2881 cgcggatacc aggcgatccg cgagggccgc cgcgtcttcg cgcagcgtca gccgcggcag
     2941 caggaccgtg aactcgtcgc cgccgatgcg gccggtgatc gcatcgccgc tcaactggtg
     3001 ccggatgcgg tccgcgacca gcttcagcag gctgtcgccg acggcatggc tgtagctgtc
     3061 attgacgaac ttgaaggcat cgaggtcgat gaacagcact accgccgagc gccggtcgcg
     3121 cgccgccagt tcgatcgcca catcgcagcg gcgctggaac tccgcgcggt tcacgaggcc
     3181 ggtcagcggg tcgtgtgccg cgaggtgctg caggcggtat tcgtcttcct tggcggccga
     3241 gatgtcgctg aacaccgcca cgtaatgcag cacgcggtgt tcggcatcgc ggatcgtgct
     3301 gatgctcagc ttttcggtga aggtggtgcc gtcgcggcgg cgactctcga cttcgccggt
     3361 ccagtggtgg gtggcgatga tctcgtccca gatcgtggcc gggagcggcc cgccatcgcg
     3421 catccggcgc gtgtcgtcga ggcgcatgcc gatcaggtgt tcgggggtga agccggtgat
     3481 ctgggtggcc gccgcgttgg ccgaaacgat ccgtcgggtg gcatccgcga tgaacacgcc
     3541 ctcggcgatg ctggccagcg cctctgccgc gatcctgcgc tcacggtcgc tctccacccg
     3601 ctcggtgatg tcgcggatga tcgcctgccg cacgagctgt tcgccccaca ccgccatgct
     3661 gctttgcgtt tcgatcggcc gggtccggcc gtgggcgtcg cgcaactcgc cgtcgcccgg
     3721 caagccgtcg cgcaatgcgc tggtttcgaa caggtcggcg taagagccgc ccatcagcgc
     3781 ctgcggttcg cgacccatcc atgccccggc ggtgcggttc gcatcgagga tgcggccgct
     3841 tcgctcgtcc accatcacga tcgcgtccgg cgcgctgtcg aacagcagcc gatagcgttc
     3901 ctcgctgccg cggatgcgcg cgagcgtgcg ccgcgccagc agcagccaga gcatgaaggc
     3961 gactgcggcc gatgtgagca cgccgacgta cagggccctg cctatcgaca ctgccccgtg
     4021 cgcgacaaac agcgaaaagt cattgctgcg cggctggatg tagtcgttga ggacgttgat
     4081 ccgccggcgc tgccggatga tctcgtcgcg ggggatcggg ccgctggcat acgcctggtg
     4141 gagttcgccg gctatggcat ggagctgcgc aatcgaatcg tccgtcgagc gccacacccg
     4201 caccgcgtcc ttcatgtagg gcgcgttgcc gaagtgctgc agcatgaaga tcacgcccgg
     4261 gatcgccacc ggcatcgcct tcgtgcggcg caattcctct tcgacctggt cgtaatcgaa
     4321 gtagcccgac tggatcttgt cgcgcgccgt ccggtaggta tcgagcacga cgaagttgcg
     4381 ctcgaaatcg cgatacgccg ccggatcgcc cgtggtcgcg tagttcagca ggtcgaccgc
     4441 cgactgtttt tgcgccttgg accagacgct ctcgccgttg aggaagccgg cgagagtgat
     4501 ctggatctgg aacgctgccc aggtgagcaa caggatgaac gccaccatgg cgccaagcgc
     4561 gtaggctagc ggcaacagtc gcttcgtcag cgggctgtgg acggcgggga aaaggccgcg
     4621 gcgcatttat tcgggatgct cagctcatgg gggctataac gctccttcct tagcaagcaa
     4681 aattcgtacc aaacagccgg tcaggcgacc gcccggtagc tttctccggc ttccatttcg
     4741 cctgtgtatt gtacacaacg atccgccagc atccgcatgc tcacgtagct gcgcttgacg
     4801 cactcctcga tgtgcgcgac gtcggctgcc gccggcgacg ttcccatgag cgtgcagacg
     4861 atttccagcg cttcccaggg atgggtgtcg tcataggcgg catggagctg cagccagcgc
     4921 aggctcgcct tgcgggtcgc ggccgggaat tgctccgcgt agctctcgct gtcatagagg
     4981 atctgcgacc attcgccggt cgcgccttcc acggcatagt tggtggcagc aatgctcgcc
     5041 gcgagcgagc cacggctgct gatctcctcg caccagttcg ccagcacctg ggttccgtgc
     5101 ggcaccacgc cgctcaacac ctgcttgcgc ggcacgcccg cgccttccgc ccacatcaac
     5161 cagtattccg cgtggttctg ctcgacgcgg atgttgcgaa ccagccagcg cctcgccagg
     5221 tcgtcgcctg tgctgcggcc atagctcgtc ttcaggaggc tgtgcgccat gtatccgggg
     5281 aaccgctcga tcaccggcca gatgccgacc atgaagttgg cggtgcactg gcgggtgggt
     5341 tcatcacgca tcacgtccca cagttcgtgg tcgacgacgg tcttcttgac gacgtcgcac
     5401 cgccctacca ggtcttgggc ccattcgggg tagctgccca gttcggtcag tggaccacta
     5461 cgttcaaacc gcacattcat gttgttctcc tcgggtcagt cgagttcttc gagttgcgtt
     5521 gcgatgggtg gagcgaacga aggctcggct ggaaccctgc cgcacctccc tgcaccgtga
     5581 ttgaatcggc cagctcctgg ataccgacgc gtatggaggt ttacgccctt cgaaaaacac
     5641 cctgcgaagc gtcgcgtaac cccgtgacat ccttgaacta cttccctgcg taattcccca
     5701 cagatcaaat cccggcgagc ttagtttact ctttgttaat ttctgcgcca ccacttttcg
     5761 tcattttttt gacgccgacc ttgatctggg tcaaaaaccg ccgccgccgc ggatccgttt
     5821 tacttaacgc caggaatcac attagttacg caacacattg atcaggcttg ttcaaagtta
     5881 taggcatggg gcgagtccct gcccgggcgg gagcacccga tcgaaggcgc aaatcggtaa
     5941 aaaaagttgg gtcgtccgtc ggaatgccgc tgtgacgcgc tcgccggttg ctccgtggcg
     6001 gcctctaggg tcgctttccg ccacttgcgg gcttccgccg gaccgcgggt tgaaacggcc
     6061 aggtggacga tcaggcctct ggatgcgtca gtgcgaaatc gattgcggcg caggcggcgg
     6121 cgatctgcgc ctgattgcac tgttcgggtg tcgcgcgcgt gctgtccggg tagacctcgg
     6181 tggtcgtcct gtaacgcgcg gcggtgacgc ccgcgcacag tccgagtcgt tccagcgcgt
     6241 actcgatcac gccgggtgcc accaccggcg acccgatgat ctcgcccttg tcgtcggctg
     6301 gtgcgatgtg cgtgaccttc gcgacggccg cgatgacggc ctgctggaat ccgggctgcg
     6361 gattcgcgct gtccgcgcaa aggtagaaac catccggcac cgtgcccggt tcataggcca
     6421 cgccgtcgcg cgccgccaag gccggacgaa attccgattc atcggtgtcg gtggtctcgt
     6481 gcaggtcgat gtgcatccat acgcgatcgc gatacggcat gaggaagcgg atcagcgccg
     6541 ccgattccgg tgccgggctg tcggcgcgaa agttgcggtt cggatcgagc gcgtacggat
     6601 tccaacgatg gatgcgctcg taaccccagg ggcttacgca cggcaccacc aggatgttgg
     6661 cgcggcctgc gtaatccgcc gcatggcgtt cggcgaattc cagggcgcca tgcacgccgc
     6721 tggtttcata gccgtggacc ccgccggtca ccagcatgat gggcgaagcg tcccgccagc
     6781 cgtgggtctt gagcgcgaac agcggatacg catcgggttc gtaatcgagt ttgccgtact
     6841 gctcgacatc gaatcgcgaa cgcaggcgtt cgatcgccgc gagcacgtcg tcggcgtagc
     6901 tgcgctgctt gtgctgccgc gagcgccact cggccttctc ggcctcgccc cagggctttc
     6961 ccggtgtacc aatcggatac ggctgcgcca tcgtgctgtg cacctcacca acaagatcga
     7021 caagcgaatt actctaccat tgcggagagg cgcaccttcg cgcgcatggc gcgctccaac
     7081 gacggtcgcg ctggttgcag gaacggccga tggccgcgat caacgttgcg gttacgcctg
     7141 tcacgtgcgt gccgagctcc tgcgacaagc gtcgtggcta caaccgcacg ctgccttcga
     7201 tgcaggtgat gcaggctccg ccgacccaga tgcgatcgcc ctcctgctcg atgtcgacga
     7261 ttccggagcg gccgaggacc gttccctggc gcgcgcgata tcgcggcggt gcggtgccat
     7321 cgccgatcag ccattgcgcc agcgaggcgt tgaggctgcc ggtgaccgga tcctcgagca
     7381 tgccatcacc ggtgaaaaag gcgcgcacct cgaaatccgc tgggcccccc gccgggtgcg
     7441 gcgcaatgac gccgacgtcg aggcccgcga gcgtggcctt gtcgatttcg agttcgaggg
     7501 cgctgcgcgc ggaatcgagc aacacgccga tccagcccgg tccattgtcg acccattgca
     7561 tggcgcgaac cttttccggt gccacgcgca ggcccgcgac gatgcgatcg gccagatcgg
     7621 cggcaaccgg gccgtcgcgc cgcaacggcg gcgcagcgaa cgccagccgc ccatcgcggt
     7681 ggcgaatgtc gatgacaccc gcgccgcatt cctggaccac ggtgcccgcg gtggc
//
LOCUS       DASSNH010000077         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_195434, whole genome shotgun sequence.
ACCESSION   DASSNH010000077 DASSNH010000000
VERSION     DASSNH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195434"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..2065
                     /gene="metG"
                     /locus_tag="VFK00_02775"
     CDS             5..2065
                     /gene="metG"
                     /locus_tag="VFK00_02775"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7591909.1"
                     /translation="MHLGHIVEHVQSDIWVRAMRMAGHTVHYVCADDAHGTSIMLRAE
                     REGITPQTLIEGVWKQHTADLHDFGVRYDHYSTTHSAANRELTYQIWRSLEKNGHLES
                     KPVKQLFDPERQMFLPDRFIKGECPVCHSKDQYGDACEVCGSTYTPMDLIDPYSVVSG
                     AKPIEKESLHFFVKLADFESLLEAWLEERRAAGGLQAEVVNKLQEWFAEGLRSWDVSR
                     DAPYFGFEIPDQPGKYFYVWVDAPVGYLAAFKELCEAKRGGLTPLDFERFTRLDADDT
                     ELVHFIGKDIIYFHTLFWPAMLHGAGLRMPTAVNVHGFLTVDGAKMSKSRGTFVNART
                     YLDHLDADYLRYYLASMLGPTLADIDLDLKAFEERVNSHLVGKWVNIASRCAGFVHKL
                     FDGRLAVSLPQAERARYAAAAQKLASCAALYEARDYAAAVRGIIEVADEANAYVAEHA
                     PWVLAKDETRRAELHAVLTLALNYFRLLAIWLAPAVPATAARAFAFLGDTPVRFDAAL
                     TPLLDHRIQPFKALATRIDPNQIAAMIEASKESLHVNQTPSPPSSPLDGGGSKASSIA
                     PGSGSGVAGPDVVSIDDFAKLDLRVARVDLCEFVEGSDKLLRFELDAGDLGKRQIFSG
                     IKAAYPEPGKLVGRNVVFIANLAPRKMRFGVSEGMILSAGTGGNTLFLLDADAGAEPG
                     MPVG"
     gene            2207..>2387
                     /locus_tag="VFK00_02780"
     CDS             2207..>2387
                     /locus_tag="VFK00_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HET7591910.1"
                     /translation="MFAVLFVACAATPWPRPADATPASTPAPRHVFVIVLENQSYRVT
                     FGPDSPAPYLARELPK"
BASE COUNT          412 a          799 c          758 g          418 t
ORIGIN      
        1 cgcgatccac ctggggcaca tcgtcgagca cgtgcagagc gacatctggg tgcgcgcgat
       61 gcggatggcc gggcacacgg tgcactacgt ctgtgccgac gacgcgcacg gcacctcgat
      121 catgctgcgc gccgagcgcg agggcatcac gccgcagacg ctgatcgaag gcgtgtggaa
      181 gcagcacact gccgacctgc acgatttcgg ggtgcgctac gaccattact ccacgaccca
      241 cagcgccgcc aaccgcgaac tgacttacca gatctggcgc tcgctcgaaa agaacggcca
      301 cctcgagtcc aagccggtca agcagttgtt cgatcccgag cggcagatgt tcctgcccga
      361 ccgcttcatc aagggcgaat gcccggtctg ccattcgaag gatcagtacg gcgacgcctg
      421 cgaggtgtgc ggcagcacct acacgccgat ggacctgatc gatccgtatt cggtggtgtc
      481 cggggccaag ccgatcgaga aggagtcgct gcacttcttc gtgaaactcg ccgacttcga
      541 atcgttgctg gaggcctggc tggaggaacg ccgcgcagcg ggcggcttgc aggcggaagt
      601 cgtcaacaag ctgcaggaat ggttcgccga ggggctccgc agctgggacg tgtcgcgcga
      661 cgcgccgtac ttcggtttcg agatccccga ccagcccggc aaatacttct acgtgtgggt
      721 ggacgcgccg gtcggctacc tcgccgcctt caaggaactg tgcgaagcga aacgcggcgg
      781 gctgacgccg ctcgatttcg aacgcttcac gcgactggac gccgacgaca ccgaattggt
      841 gcatttcatc ggcaaggaca tcatctattt ccatacgttg ttctggccgg cgatgctgca
      901 cggcgcgggg ctgcgaatgc cgaccgcggt gaacgtgcac ggcttcctga ccgtggacgg
      961 cgccaagatg tcgaagtcgc gcggcacgtt cgtcaacgcg cgcacctatc tcgatcatct
     1021 cgacgccgac tacctgcgct actacctcgc ctcgatgctg ggtccgacgc tggcggacat
     1081 cgacctcgac ttgaaagcct tcgaggagcg cgtcaattcg cacctcgtcg ggaagtgggt
     1141 gaacatcgcc agccgctgcg cgggcttcgt gcacaagttg ttcgatggca ggctcgcggt
     1201 ttcgctgccc caagcagagc gcgcccgcta cgccgcggcg gcgcagaagc ttgcttcctg
     1261 cgcggcgctc tacgaagcgc gcgattacgc cgccgcggtg cgcgggatca tcgaagtcgc
     1321 cgacgaagcc aacgcctatg tcgccgagca tgcgccgtgg gtgttggcca aggacgaaac
     1381 ccggcgcgcc gaactgcacg cggtgctgac gctcgcgctc aattatttcc ggctgctggc
     1441 aatctggctg gcgccggccg tgccggcgac cgcggcgcgt gccttcgcgt tcctcggcga
     1501 cacgccggtg cgtttcgacg cggcgctcac gccgttgctc gatcaccgga tccagccctt
     1561 caaggcgctc gccacccgca tcgaccccaa ccagatcgcc gccatgatcg aagcttcgaa
     1621 ggaatcgctg cacgtgaatc aaaccccatc cccgcccagc tctccccttg acggaggggg
     1681 ctcgaaagcg agctccatcg cgcccggctc cgggtccggg gtcgcgggtc cggatgtcgt
     1741 ctccatcgac gacttcgcca agctcgacct gcgggtcgcc agggtcgacc tctgcgaatt
     1801 cgtggagggg tccgacaagc tgctccgctt cgaactggac gccggcgacc tcggcaagcg
     1861 ccagatcttc tcgggcatca aggctgccta tcccgaaccc ggaaaactgg tcggccgcaa
     1921 cgtggtgttc atcgccaacc tggcgccgcg caagatgcgt ttcggggtga gcgagggcat
     1981 gatcctttcg gcaggaacag gcggcaacac attgtttttg ctggacgccg acgccggcgc
     2041 agaaccggga atgccggtcg gatagagacc gcgccgcacg acattcgtgc ttccgcgcat
     2101 tttgctatcg tggcggattc caactgcggg gaggcttcgc acgatgggca ataccctggg
     2161 catcggtcgc gggccgtttg agtttcccgg tcgcacggca acgttgatgt tcgctgtcct
     2221 tttcgttgcg tgcgctgcaa cgccatggcc acgcccggcg gatgccacgc ccgcatcgac
     2281 gcccgcgccc cggcacgtct tcgtgatcgt gctggagaac cagtcgtacc gcgtcacctt
     2341 cggaccggat tcgccggcgc cctacctcgc gcgcgaattg ccgaaac
//
LOCUS       DASSNH010000078        15272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_206029, whole genome shotgun sequence.
ACCESSION   DASSNH010000078 DASSNH010000000
VERSION     DASSNH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206029"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="uvrC"
                     /locus_tag="VFK00_02785"
     CDS             complement(<1..885)
                     /gene="uvrC"
                     /locus_tag="VFK00_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7591911.1"
                     /translation="MESDAAAFDGKAFVRTLTTSPGVYRMFDADDALLYVGKARNLKK
                     RVGSYFLKPQLEPRIASMVSQIARIEVTLTRTEGEALLLEAQLIKSLKPRYNIMLRDD
                     KSYPYIFVSDDPFPRIVFHRGAKREKGHYFGPYPSAHAVRESIALMQRLFRIRNCENS
                     YYRNRTRPCLQYQIGRCTAPCVDFIGQQEYAEDVRHAVMFLEGRNTRVLDELGAAMDA
                     ASARQDYERAARLRDQVATLRSLQAHHYVRGASADMDVFACRVEAGVACVSVLFFRNG
                     ISLGSRDHFPKLSFDSTPA"
     gene            complement(970..2427)
                     /locus_tag="VFK00_02790"
     CDS             complement(970..2427)
                     /locus_tag="VFK00_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591912.1"
                     /translation="MIPGDVAFAPHATSRPPAAEFPESLRWVNRSNRVRLADWRGQIV
                     LVLFWNSASASSINLLGELRQLEKRLPGAFVALCVHTPRYALQQSDVCVLKAAHRNRL
                     RMAVANDHDWRAWQQFAIGAWPTTLLIDPEGGLAARLGGEGRCQEIEDAIVQLRDEQA
                     LHGSAAPDEVPRETRPEPGGALTFPAHAVATETRLFVSDTGHHRILECSHDGRVLRQF
                     GSGTPGSWDGQLAACGLQLPQGLAVDRHALYVADTGNHCVRRIRLDTGDVETVLGSGR
                     PGYSNVEEQGGGLRASINAPHALVASDDVLYVAATGQHQVLRADLRHQRVETLAGDGR
                     SDVRDGIGGQSSLSQPMALALVSGQLMIADAGGNAIRRLRFADLALTTLAGSSPWEPG
                     NRDGVGDKARFAYPCGLAAGENRLYVADTMNDRLCTLDPYSGQLATMAVEWVLHEPQG
                     LSYAAGSLWVADRNDHAILRIDPARGTCERVPVDE"
     gene            complement(2424..2936)
                     /locus_tag="VFK00_02795"
     CDS             complement(2424..2936)
                     /locus_tag="VFK00_02795"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET7591913.1"
                     /translation="MTQPPPGILFVCLGNICRSPTAEYVAKAEFARRGLRIPISSRGL
                     GDWHVGQGADPRAVAAARTEGYDLTPHRARQFHDEDFNKFATVLAVDHATLAELRRRV
                     LNDMPVPERFLVAAGLAAPASRDRGDVPDPYTGTADDFRAVLDLIRRGVDALAERLQT
                     GAGLQRGVGQ"
     gene            complement(2933..3715)
                     /gene="kdsB"
                     /locus_tag="VFK00_02800"
     CDS             complement(2933..3715)
                     /gene="kdsB"
                     /locus_tag="VFK00_02800"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET7591914.1"
                     /translation="MSGNADFIVAIPARFGSTRLPGKPLRLLAGEPLIVHVARRARAA
                     GAAGVIVATDDRRIGDALAGSGIEVCMTRADHVSGSDRLAECARIQGWADDALVVNLQ
                     GDEPFAPAGGIRAVADALREEDAPMTTLASPIKTTAEWFDPNAVKVVCDLRGRALYFS
                     RAPVPWARDALARDRTVVPPGLAVLRHIGIYGYRAGFLKAFAELPPTPLERSEALEQL
                     RALEHGHSILVRVTPEAFPPGIDTPDDLARAEHALRMTEAAP"
     gene            complement(3712..3921)
                     /locus_tag="VFK00_02805"
     CDS             complement(3712..3921)
                     /locus_tag="VFK00_02805"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HET7591915.1"
                     /translation="MALQMRSGCERCRAALAPDGSAYICVYECTFCAGCADALRYVCP
                     NCGGELVPRPRPNPRPAGEDGQAGA"
     gene            complement(3921..5582)
                     /locus_tag="VFK00_02810"
     CDS             complement(3921..5582)
                     /locus_tag="VFK00_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591916.1"
                     /translation="MDSYQRLIEDLPARLPPGRSRRGGEKATREAVAVLPLANPEQAG
                     RELEQILDGMLATTWAGGDRIAALEHLRMPLVNLCAGFEVRSTAESHPMSEAAAAGAD
                     MAQRLQRKLVWGYALGLHELCAPAGKVPRFRTRTAALAVVRALAHADQALLWAYRRYQ
                     SPPAGVWRRIHALHAFACELGVADEIVDDALGGVPLTARAVYAQLLLLAMSNPYRFSA
                     RELQEARLVVRCVAGQLGLEPAGTKGIGVDTDSDTGPGCIPEERLHAGPGVQAVDVAP
                     VQRVFDERIALLPPGVDPIDLPQPGGGRFTTSVAFLGRLRAGWEAAPRGHIRLAARHV
                     LDFVVGMHALHYALAGNMEFATFVRAVHGDAIMVGEHELASAWLAASDASRPRIFRGE
                     VMDQSEGGYRMRVHAADGLRMRVGELVGLAPVDEEADERDWMVGVIRWLRHEGDEDLL
                     GIELLRRGARAAGMRPVTADGEALAPLRAVELLDGEDADRLSLLVTHRLARHVVAAEV
                     ALPALATDWNSHASIGTWRTATTEALAPACFRVTLERSVASERMA"
     gene            5694..7142
                     /locus_tag="VFK00_02815"
     CDS             5694..7142
                     /locus_tag="VFK00_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7591917.1"
                     /translation="MHGPRQSPPTAFRPLAEQALSRAAGAPLSTGNRVDLLIDGRANF
                     DAWLEAIASSRHNILFGNYIFRNDEVGREFVQALAERARAGVRVHVLHDWLGCLGQSR
                     ARFWQPLREAGGEVRVYNPFDPLSPFGWVNRDHRKLLTVDGRIGFVSGVCVSALWLGD
                     TARGRDPWRDTGIAVHGPAVTDLENAFADTWKMLGEPIEPGDAPADEAAAAAGDVALR
                     VIATQPSTAGTYRLDQLIASLARERLWLTDAYFIGVAPYLSALTNAAQDGVDVRLLVP
                     GASDIPMVAALSRTGYRALLQAGIRIYEWNGPMLHAKTAVADDRWARVGSTNLNVASW
                     LGNCEIDVAIEHTGFAELLAGQYESDLEHATEIVLSRRRARPAKPPPRHGFHGHGSAG
                     RSAVGAMRMATTLGAALGNRRVLSDTESRPLLGGALFLLLIAAVAALWPWALAWPLGA
                     LAAWIGISLAVRWARLQWRRRARRHATPTLKK"
     gene            complement(7126..7530)
                     /locus_tag="VFK00_02820"
     CDS             complement(7126..7530)
                     /locus_tag="VFK00_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7591918.1"
                     /translation="MRFAVIAAGTAFATVLLAGCASSPGTKQPPSMRWIAPPAAGADH
                     LPSIDITYKNQHPPHYPMSAVVAHHQGRVVLAVFVDAQSRVSKVEVERSSGYPELDAA
                     AAAAAQDWRFAAGVKDGEPYASVVLVPVTFSM"
     gene            7627..8955
                     /gene="xseA"
                     /locus_tag="VFK00_02825"
     CDS             7627..8955
                     /gene="xseA"
                     /locus_tag="VFK00_02825"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7591919.1"
                     /translation="MLTPSALNRLVRELIEGALPMVWIEGEICNFVRAGSGHWYFSLK
                     DAAAEVRCAMFARENARLRFRAGNGTRVLARARVSLYEPRGSFQLIVEDMEDAGEGAL
                     RRAFELLKAKLQAEGLFDPERKRPLPAFPRRLGVITSASGAAVRDVLSVARRRFVLTE
                     VEVLPVLVQGTEAPAQVAAMLRKAGSAGRYDVLLVTRGGGSLEDLAAFNDEGVARALA
                     ASPVPVVSAVGHEIDFSICDFVADMRAPTPSAAAELILPDGDALAKTLAHLEARSRHA
                     WSRHLQAAMQRLDHGCTRLNAQHPGRRLALGRERMLALRERMLRCEALGRERAAMRVG
                     KAYDRLFSRHPAARLEQLDRRGSELSRRLAAAWQHAAERRRQRLVELARALKAVSPLD
                     VLKRGYAILFDESGTVLRSVRDTRAGAALRARLADGELPLRVETPPDTPA"
     gene            8999..10444
                     /locus_tag="VFK00_02830"
     CDS             8999..10444
                     /locus_tag="VFK00_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7591920.1"
                     /translation="MSETPRLKSHYPYYLANEPVQANADLEVLDKYSGEVATRVAVAD
                     AQAIDAAITAAATAAAPMAAMKPYQRREVLEHCVTRMRERKDELAYALCVEAGKPIRD
                     SQGEAERLIDTFRIAADESTRIEGEIVNLEISERTRGYRGFVKRVPLGPCSFITPFNF
                     PLNLVAHKVAPAIAAGCPFVLKPSERTPIGALIIGEVLAETDLPAGAFSILPCRIEDA
                     DPFIEDERLKLLSFTGGQIGWELKARAGKKKVVLELGGNAACIVDADQKPRLDFVVSR
                     LISGSFYQSGQSCIGVQRILVHASLYEDVKKRFVAAATELKTGDPKDPETFLGPMIDE
                     AAAKRMEDWIAEAKKAGAAVLCGGERNGNMLDATVLENVPSAASINRMEAFGPVALLQ
                     PFDDFDAALGVVNDSEYGLQAGLFTDNLGHAMRAWDALVEGGVIVGDIPSFRVDNMPY
                     GGVKLSGFGREGIRYAIDDMSERRLLVIRDP"
     gene            10538..12307
                     /locus_tag="VFK00_02835"
     CDS             10538..12307
                     /locus_tag="VFK00_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET7591921.1"
                     /translation="MTDTADRRRPIRQLAALGALWPFMRRHRALAFGWLFFLALSSAA
                     TLSLPVAVRHMIDRGFASHDSALVNETFLVLFVVALVLAIATAARYFCISLLGERAIA
                     ALRDLLYSRIIRLDVAFYEKNRVGELISRLGTDTEVVQTLIGSSISIALRSAVTVIGA
                     AALMVWTSPRLAGLTGLVIPVVIVPIALYGRRLAKLSRTNQDRIADTSAIANETLNSV
                     YAVKAYARERIESSRYADAVQRALHAAQQRIAARAVLTGGVIVLIFGAITLVLWAGAR
                     DVLAGSMDAGLLSQFVLYAVMAAGSVGALGGVWGDVLRAAGSMGRISELLAEHPSIAD
                     PAQPTPLPKPARGEIAFEHVEFHYPSRPDTLALHDFSLRIRRGETVALVGPSGAGKST
                     VFNLLLRFHDPQRGRITLDGVDLRSLELDALRGALALVPQETVIFGASAADNIRFGRA
                     DADDASVHAAARAAEAFEFLDGQPEGFDTQLGERGVRLSGGQRQRIAIARAILKDAPV
                     LLLDEATSSLDAQSEAAIQAALERAGRGRTTLVIAHRLATVLRADRIVVMDAGRIVAD
                     GNHETLLREGGLYAELARLQFAA"
     gene            complement(12340..12570)
                     /locus_tag="VFK00_02840"
     CDS             complement(12340..12570)
                     /locus_tag="VFK00_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7591922.1"
                     /translation="MAVGTVKWFNDAKGFGFITPDGGGADVFVHFSAIRAQGFRSLKE
                     GQRVQFEVVQGPKGMQAAEVTAEGATAPAAPA"
     gene            complement(12805..13575)
                     /locus_tag="VFK00_02845"
     CDS             complement(12805..13575)
                     /locus_tag="VFK00_02845"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HET7591923.1"
                     /translation="MNTPSHPPLPDLAIIGGTGVYAFPGLGHPVRHEVATRWGAPSSA
                     IAAGEVEGRTVAFLARHGETHAIAPHRINYRANIAALHELGCRQVVGINAVGGIRDDM
                     APRVLAIPDQLIDYTCGRAASFSDADGEAVLHVEFGDPYSRSLRRALLSAAAAAGIEV
                     VDGGCYGATQGPRLETRAEIERMRRDGCDLVGMTGMPEAALARELGMEYACLAVVANW
                     AAGCGPQPDVPIELAEIHAHLAAASGRVPAIVRALLAD"
     gene            complement(13669..14238)
                     /locus_tag="VFK00_02850"
     CDS             complement(13669..14238)
                     /locus_tag="VFK00_02850"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HET7591924.1"
                     /translation="MSAPAYPLGAVLPRAEQLHRRDALEAAIARMGAGITAELDGERA
                     VFLTVMNGGLFFAAPLALAIECDLEFDYVHATRYGEATVGGGVEWLHRPRVALAGRTV
                     ILVDDILDEGLTLAALRAHCLAAGARRVLIAVLCVKRHDRRVPDLVADFAGVEVPDRY
                     VFGYGLDYHGQGRNLPDIYALEEAGVADG"
     gene            complement(14235..15254)
                     /gene="nagZ"
                     /locus_tag="VFK00_02855"
     CDS             complement(14235..15254)
                     /gene="nagZ"
                     /locus_tag="VFK00_02855"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7591925.1"
                     /translation="MLIIGIEGTELTNGDRVRLAAPEVSGAILFTRNYRDHDQLAGLV
                     ASIRAARGDPFLLSVDHEGGPVQRFRPGFTRLPPLARIGELFHQDRDAAIAMAEMHAI
                     VMATEVRACDIDLSFAPVVDLARGNRVIADRAFDPDPAVVGELAAAYVRGMRLAGMAA
                     TVKHYPGHGSIPEDTHLEAAIDPRPLAEIRETDLVPFVDGFAAGAEAVMVAHVTYPAV
                     DARAAGYSRKWIEEVLRGDLGFRGVVFSDDVGMAAAEAAGGIGARVTAHLDAGCDLVL
                     VCAPAMVDDAIESVRGRTPCLAENVAALCGSVAAGWGALQADPQHARYVAALDDLAAH
                     SEPRA"
BASE COUNT         2423 a         5352 c         5136 g         2361 t
ORIGIN      
        1 cgcgggcgtg gaatcgaaag acagtttggg gaaatggtcg cgcgagccga ggctgatgcc
       61 gttgcggaaa aacagcaccg acacgcacgc cacgcccgcc tccacccggc acgcgaacac
      121 gtccatgtcg gcgctcgcgc cacgcacgta atggtgcgcc tgcaggctgc gcaaggtcgc
      181 gacctggtcg cgaaggcgag cggcgcgctc gtaatcctgg cgggcgctcg cggcgtccat
      241 cgcggcgccg agttcatcga gcacgcgggt gttgcggccc tcgaggaaca tcaccgcgtg
      301 gcgcacatcc tccgcatatt cctgctgtcc gatgaaatcg acgcaaggcg cggtgcaccg
      361 gccgatctgg tattgcaggc acggccgggt acggttgcgg taatagctgt tctcgcaatt
      421 gcggatacgg aacagccgct gcatcagcgc gatgctttcg cgcaccgcgt gcgcgctcgg
      481 atacggaccg aagtaatggc ccttttcgcg cttggcgccg cggtggaaca cgatgcgcgg
      541 aaacggatca tccgacacga agatgtacgg ataactcttg tcgtcgcgca acatgatgtt
      601 gtagcgcggc ttcagggact tgatgagctg ggcttccagc agcagcgcct cgccctcggt
      661 gcgcgtcagc gtgacttcga tgcgcgcgat ctgcgacacc atcgaggcga tccgcggttc
      721 gagctgcggc ttcaggaaat agctgcccac ccgcttcttg aggttgcgcg ccttgcccac
      781 gtacagcagc gcgtcgtccg catcgaacat gcggtacacg ccgggcgagg tggtgagggt
      841 gcgaacgaag gccttgccat cgaaggccgc tgcatcggac tccatcgcgg aattgtatcc
      901 gcggcggccg gcgcggcttt gcccctcgtc aaacgcgcgg cacgcgaaga atcggccccg
      961 acgcggcgat cattcgtcga ccggcacccg ctcgcaggtg ccgcgcgcgg ggtcgatccg
     1021 gaggatcgcg tggtcgttgc ggtccgcgac ccacagcgag ccggcggcgt acgacagtcc
     1081 ctgcggctcg tgcagcaccc actcgactgc catcgtcgcg agctggccgc tgtagggatc
     1141 gagggtgcat agccggtcgt tcatggtgtc ggccacgtac aaacggtttt cgccggcggc
     1201 cagaccgcac ggatacgcga agcgggcctt gtcgccgaca ccgtcgcgat tgccgggctc
     1261 ccagggggag gatcccgcca acgtggtcag cgcgaggtcg gcgaaccgca gtctgcggat
     1321 cgcattcccg ccggcatccg cgatcatcaa ttgacccgag accaacgcca atgccatcgg
     1381 ttgcgacagc gacgactgcc cgccgatgcc gtcgcgcacg tcgctgcgcc cgtcgccggc
     1441 gagcgtttcc acccgctggt ggcgaaggtc cgcccgcagg acctggtgct gccccgtggc
     1501 cgcgacgtac agcacgtcgt cgctcgccac gagggcgtgc ggcgcattga tcgaggcgcg
     1561 caatccgccc ccctgctcct cgacgttgct gtagcccggc cggccggatc cgaggaccgt
     1621 ttcgacatct cccgtgtcca gccggatgcg tcggacgcaa tggttgccgg tgtccgccac
     1681 gtacaacgca tggcggtcga ccgccaggcc ctgcggcagc tgcaagccgc aggccgccag
     1741 ttggccatcc cagcttccgg gcgtgcccga tccgaactgc cgcagcaccc gcccgtcgtg
     1801 gctgcattcg aggatgcgat ggtgcccggt gtcgctgacg aacaagcgtg tttccgttgc
     1861 gaccgcgtgt gcggggaatg tgagcgcacc gcccggttcg ggtcgcgtct cccgcgggac
     1921 ctcgtccggc gctgcgctgc catgcagggc ctgttcgtcg cgcaactgca cgatcgcgtc
     1981 ctcgatttcc tggcagcgtc cctccccgcc aaggcgcgcg gcaaggccgc cttcgggatc
     2041 gatcagcaag gtggtcggcc acgctccgat cgcgaactgc tgccacgcac gccagtcgtg
     2101 gtcgttggca actgccatgc gcaggcggtt gcgatgggcg gccttcagga cgcagacgtc
     2161 ggattgctgc aacgcatagc gtggcgtgtg cacgcacaac gcgacgaacg cgccgggaag
     2221 tcttttttcg agttgccgca actccccgag caagttgatg ctgctggcac tcgcgctgtt
     2281 ccagaacagc accagcacga tctgcccgcg ccagtcggcc aagcggacgc ggttcgaccg
     2341 attgacccag cgcaagctct cgggaaactc cgccgcgggc ggacgcgatg tcgcgtgcgg
     2401 ggcgaaggcg acgtcgccgg ggatcattgg cccacccccc gctgcaggcc tgcaccggtt
     2461 tgcaaccgct cggccagggc gtccacgccg cgccggatca ggtccagcac cgcgcggaaa
     2521 tcatcggcgg ttccggtgta cgggtcgggc acgtcgccac ggtcgcgcga tgccggcgcg
     2581 gcgaggcccg cggcaacgag gaagcgttcg ggcacgggca tgtcgttcaa cacccgccgc
     2641 cgcaactcgg cgagggtcgc gtgatcgacc gcgagcaccg tcgcgaattt gttgaagtcc
     2701 tcgtcgtgga actggcgtgc ccgatgcggc gtgaggtcgt agccttcggt gcgcgcggcc
     2761 gcgaccgccc gcgggtccgc gccctggccg acgtgccagt cgcccaggcc acgcgaggaa
     2821 atggggatgc gcagcccgcg cctggcgaat tcggccttcg ccacgtactc ggccgtcggc
     2881 gagcggcaga tgttgccgag gcagacgaac aggatcccgg gcggcggctg cgtcatggcg
     2941 ctgcctcggt catgcgcagc gcgtgctcgg cacgcgcgag gtcgtccggc gtatcgatgc
     3001 caggggggaa cgcttcgggc gtcacccgca ccaggatcga atggccgtgt tccagcgcgc
     3061 gcaactgttc gagcgcctcg ctccgctcga gcggggtggg cggcaattcc gcgaacgcct
     3121 tgaggaagcc tgcgcggtat ccgtagatgc cgatatggcg cagcaccgcg agtcccgggg
     3181 ggaccacggt gcgatcgcgc gccagtgcat cgcgcgccca cggcaccggt gcgcgcgaga
     3241 agtacaacgc gcggccgcgc aggtcgcaga ccaccttgac ggcattcgga tcgaaccact
     3301 ccgccgtcgt tttgatgggc gaggcgagcg tcgtcatcgg cgcgtcttcc tcgcgcaagg
     3361 catcggcgac cgcacggatg ccgcccgccg gcgcgaacgg ttcgtcgccc tgcaggttga
     3421 ccaccagcgc gtcgtcggcc caaccctgga tccgtgcgca ttcggccagg cgatcgctgc
     3481 cgctcacgtg gtcggcccgc gtcatgcaga cttcgattcc gctgccggcg agcgcgtcgc
     3541 cgatgcgccg gtcatcggtg gcgacgatca cacccgctgc gcccgctgcg cgcgcgcgcc
     3601 gtgcgacgtg gacgatcagg ggctcgcctg ccagcaaccg caacggcttg cccggcaggc
     3661 gcgtggagcc gaagcgtgcc ggaatcgcga cgatgaagtc cgcattgccg ctcacgcgcc
     3721 agcctgcccg tcctcgcctg ccgggcgcgg gttcggccgt ggccgcggaa ccagttctcc
     3781 gccacagttg gggcagacat accgcaaggc gtcggcgcag ccggcgcaga aggtgcactc
     3841 gtacacgcag atgtaggcgc tgccgtccgg cgccagcgcc gcccggcagc gctcgcaccc
     3901 actgcgcatc tgaagtgcca tcacgccatc ctttccgacg ccacggaacg ctcgagggta
     3961 acgcgaaaac acgcgggcgc cagtgcttcg gtcgtggccg tgcgccaggt tccgatcgac
     4021 gcgtgcgagt tccagtcggt ggccagcgcc ggcaacgcca cttcggccgc gacgacgtgg
     4081 cgggcgaggc gatgcgtcac cagcaaggac aatcggtcgg cgtcctctcc atccagcaac
     4141 tccacggcgc gcaacggcgc cagcgcctcc ccatcggcgg tcaccggccg catgcccgcg
     4201 gcgcgcgcac cccggcgcag caattcgata cccagcaagt cttcatcgcc ttcgtgccgc
     4261 aaccaacgga tcacgcccac catccagtcg cgttcgtcgg cctcctcgtc gaccggggcg
     4321 aggccgacca gttcgccgac ccgcatccgc aacccgtccg cggcatgcac gcgcatccgg
     4381 tagccgccct cgctctggtc catgacttcg ccacgaaaga tacgcgggcg cgaggcatcc
     4441 gaagccgcga gccaggccga ggcgagttcg tgctcgccca ccatgatcgc gtcgccgtgc
     4501 accgcccgca cgaaggtcgc gaactccatg ttgccggcga gcgcgtaatg cagtgcgtgc
     4561 atccccacca cgaaatcgag cacatgccgg gccgcgaggc ggatatggcc gcgcggcgcg
     4621 gcttcccagc cggcgcgcaa ccgcccgaga aatgccacgc tggtggtgaa gcggccgccc
     4681 cccggttgcg gcaggtcgat gggatccacg cccggtggca gcagcgcgat cctttcgtcg
     4741 aacacccgtt gtaccggcgc gacgtccacc gcctgcacgc cgggaccggc gtgcaggcgc
     4801 tcctcgggta tgcagcctgg cccggtatca ctgtcggtgt cgacgccaat ccccttggtt
     4861 cccgcgggct ccaggccgag ttgccccgcc acgcaacgga ccaccagccg cgcctcctgg
     4921 agctcgcgcg cggaaaaccg gtacggattg ctcatggcga gcagcaacaa ctgcgcatac
     4981 acggcgcgtg ccgtcagcgg cacgccgccc agcgcatcgt ccacgatttc gtcggcgacc
     5041 cctagttcgc aggcaaatgc atgcagcgcg tggattcgcc gccacactcc cgccggcgga
     5101 gattgatagc ggcgatatgc ccacagcagc gcctggtccg catgcgcgag cgcgcgcacg
     5161 acggcgagtg ccgcggttcg cgtcctgaac ctcggcacct ttccagccgg cgcgcacaat
     5221 tcatgcaatc ccaacgcgta gccccaaacc agcttccgct gcaggcgctg cgccatgtcc
     5281 gcccctgccg ccgccgcctc ggacatcggg tgcgactcgg cggtggatcg aacctcgaac
     5341 ccggcgcaca ggttcaccag cggcatgcgc aggtgttcga gcgcggcgat ccggtcgccg
     5401 cccgcccagg tcgtcgccag catgccatcg aggatctgtt cgagctcgcg gccggcttgc
     5461 tccgggttcg cgagcggcaa taccgcgaca gcctcgcgcg tggctttctc gccgccgcgg
     5521 cgggaccgcc ccggcggcag gcgcgcaggc aggtcttcga tcaatcgttg ataactatcc
     5581 atccggactt cgattccctc ggtgcagggc cggctcgcgg agccgacact acttgccatt
     5641 ttagccttga acctgaaacc aaggccgccg aacatggacg cgtgaaaacc gtcatgcacg
     5701 ggccgcgcca atcgccgcct acggcgttcc gcccgctcgc cgagcaagcc ctgtcgcgcg
     5761 cggcgggcgc gccgctgtcc accggcaatc gcgtggatct gctgatcgac gggcgtgcga
     5821 atttcgacgc ctggctcgaa gccatcgcaa gttccaggca caacatactg ttcggaaact
     5881 atattttccg gaacgacgaa gtcggacggg aattcgtgca ggcgctggcg gaacgggccc
     5941 gcgccggggt gcgggtgcac gtgctgcacg actggctcgg gtgcctcggc caatcgaggg
     6001 ccaggttctg gcaaccgtta cgggaagcgg gcggcgaggt gcgggtctac aacccgttcg
     6061 atcccttgag tccattcggc tgggtcaacc gcgatcaccg caagttgctg acggtcgacg
     6121 gccgcatcgg cttcgtctcc ggcgtctgcg tcagcgccct gtggctgggc gatacggcac
     6181 gcggccgcga cccttggcgc gacaccggca tcgccgtgca cggcccggcg gtcacggacc
     6241 tcgagaacgc cttcgccgac acctggaaga tgctcggcga gccgatcgaa ccaggcgacg
     6301 cgcccgccga cgaggccgcc gccgctgcgg gcgatgtcgc gctccgggtc atcgcgaccc
     6361 agccttccac tgccggcacc taccgcctgg accagttgat cgcgtcgctg gcgcgggagc
     6421 gcctgtggct gaccgacgcc tatttcatcg gcgttgcgcc ctacctcagc gcgctgacca
     6481 atgcggcgca ggacggcgtc gatgtgcgac tgctggtgcc cggcgcgtcc gacattccca
     6541 tggtcgccgc cctgtcgcgc accggttacc gggcgctgct gcaagccggg atccgaatct
     6601 acgaatggaa cggtccgatg ctgcacgcca agaccgccgt ggccgacgat cgctgggccc
     6661 gggtgggttc gaccaatctc aatgtcgcga gctggctcgg caattgcgag atcgacgtgg
     6721 cgatcgagca caccggcttt gccgaactgc tggccgggca atacgaaagc gatctcgaac
     6781 acgccaccga aatcgtcctg tcgcgtcggc gcgcccgccc ggccaagccg cccccgcggc
     6841 acgggttcca tggccacggc agtgccggcc gcagtgcggt cggggcgatg cggatggcga
     6901 cgaccctcgg cgccgcgctg ggaaaccgtc gcgtgttgag cgacactgaa agccgcccgc
     6961 tgctgggcgg tgccctgttc ctgctgctga tcgccgcggt ggctgcgttg tggccgtggg
     7021 cgctggcctg gcccctgggc gcgctggccg cctggatcgg catcagcctg gcggtccggt
     7081 gggcgcggct gcaatggcgt cgccgcgcca ggcggcacgc cacgcctaca ttgaaaaagt
     7141 aaccggtacc aggacgacgc tggcgtaagg ctctccgtcc ttcacgcccg ccgcgaatcg
     7201 ccaatcctgc gcggcagcgg cggcagcggc atccagttcc ggatagccgc tcgatctctc
     7261 cacttccacc ttgctgaccc tgctctgcgc atcgacgaac accgcgagca caaccctccc
     7321 ttgatgatgc gcgaccaccg cggacatcgg atagtgcggc gggtgttggt tcttgtacgt
     7381 gatgtcgatg ctgggcaggt gatccgcacc ggctgctggc ggcgcaatcc aacgcatgct
     7441 gggaggttgt ttcgtcccgg gtgatgaagc acagcctgcg agcagcacgg tcgcgaaggc
     7501 agtaccggct gcaatcactg caaaccgcat ggattcgctc ccggcatgcc cgagcccacg
     7561 tggcgatgga cctatagtac gcaaatggcc gcaaaatctc cctccgctga ttcacccgga
     7621 cggcacgtgc tgacgccgtc tgcccttaac cggctggtac gcgagctgat cgagggcgcg
     7681 ctgccgatgg tctggatcga gggcgagatc tgcaacttcg ttcgcgccgg atcggggcac
     7741 tggtatttct cgctgaagga tgccgcagcc gaggtgcgct gcgcgatgtt cgcgcgcgaa
     7801 aacgcgcggc tccggttccg ggccggcaac ggcacgcggg tactggcacg cgcccgggtg
     7861 agcctctacg agccgcgcgg cagtttccag ttgatcgtgg aggacatgga agacgcgggc
     7921 gaaggtgcgc tgcgccgcgc gttcgaattg ctgaaggcca agctgcaggc cgaagggctg
     7981 ttcgatccgg agcgcaaacg gccgctaccg gcattcccgc gccgcctcgg ggtcatcacc
     8041 tccgcgagtg gcgcggcggt gcgcgacgtg ctgtccgtgg cgcggcgccg attcgtgttg
     8101 acggaagtcg aggtgctgcc ggtgctggtg caggggaccg aagccccggc gcaagtcgcg
     8161 gcgatgttgc gcaaggcggg gagcgccggg cgctacgacg tgctgctggt gacccgcggc
     8221 ggcgggtcgc tcgaggacct cgcagccttc aacgacgaag gcgtggcgcg tgcgctcgcc
     8281 gcgagccccg tgccggtggt ctcggcggtg ggccacgaga tcgatttctc catctgcgac
     8341 ttcgtggcgg acatgcgcgc accgacgcca tctgcggcgg cggagctgat cctcccggat
     8401 ggcgacgcgc tcgccaagac gctggcgcat ctcgaggcgc gttcccgcca cgcgtggagc
     8461 cgccatctcc aggcggcgat gcagcggctc gaccacggct gcacgcgctt gaatgcgcaa
     8521 catccggggc ggcgcctcgc gctgggacgc gagcggatgc tggcgctgcg cgagcggatg
     8581 cttcgctgcg aagcgctcgg ccgcgaacgc gcagccatgc gcgtgggcaa ggcatacgac
     8641 cggctgttct cccggcatcc ggccgcgcgc ctcgagcaac tcgatcggcg cggctccgag
     8701 ctttcgcggc gcctcgcggc ggcgtggcag catgcggccg aacgacgccg gcagcggctg
     8761 gtggaactcg cgcgggcact caaagcggtc agccccctgg atgtcctgaa gcggggctat
     8821 gccatcctgt tcgacgaatc cggcacggtg ctgcgttccg tgcgcgacac acgcgcgggc
     8881 gccgcacttc gcgcccgcct cgccgacgga gaactgccgc tcagggtgga aacgccaccc
     8941 gacacgcccg cttgacggga cgcgacgtaa gtttcccttt tcatccaggt cgcccgccat
     9001 gtccgagacg cccagactga agtcgcacta tccgtattac ctcgcgaacg agccggtgca
     9061 ggccaatgcc gatctcgagg tcctggacaa atactcgggc gaagtcgcga cacgcgtggc
     9121 cgtggccgac gcccaggcca tcgatgcggc aatcaccgcc gctgccacag ccgccgcacc
     9181 gatggccgca atgaagccct accaacgacg cgaggtgctc gagcactgcg tcacccgcat
     9241 gcgcgagcgc aaggacgaac tcgcctacgc gctctgtgtc gaggcgggca agccgatcag
     9301 ggattcgcag ggcgaagccg agcgcctgat cgacaccttc cggatcgccg ccgacgagtc
     9361 cacccgcatc gagggcgaaa tcgtcaacct ggaaatttcc gaacgcacca ggggctaccg
     9421 cggcttcgtc aagcgcgtgc cgctgggccc gtgttcgttc atcacgccgt tcaatttccc
     9481 gctgaacctg gtcgcgcaca aggtcgcgcc cgcgatcgcc gcgggctgtc cgttcgtcct
     9541 gaagccctcc gagcgcacgc cgatcggcgc gctgatcatc ggcgaggtgc tggccgaaac
     9601 cgatctcccg gcgggcgcgt tttcgatcct gccgtgtcgc atcgaggatg ccgatccgtt
     9661 catcgaggac gagcggctga aactcctctc gttcaccggc ggccagatcg gctgggaact
     9721 gaaggcacgc gccggcaaga agaaggtggt gctggaactc ggcgggaatg ccgcttgcat
     9781 cgtcgatgcc gatcagaagc cacggctgga tttcgtggtc tcgcggctga tctccggcag
     9841 tttctaccaa tcagggcaaa gctgcatcgg ggtgcaaagg atcctcgtgc acgcgtcgtt
     9901 gtacgaggat gtgaagaagc gcttcgtcgc cgccgcgacg gaattgaaga ccggcgatcc
     9961 caaggatccc gaaaccttcc tcggaccgat gatcgacgag gctgccgcga agcggatgga
    10021 ggactggatc gccgaagcga agaaggccgg cgcggcagtc ctgtgcggcg gcgagcgcaa
    10081 cggcaacatg ctggatgcga cggtgctgga aaacgtcccg agcgccgcgt cgatcaaccg
    10141 catggaggcg ttcgggccgg tcgcgctgct ccagccgttc gacgatttcg atgccgcgct
    10201 cggcgtggtc aacgattccg agtacggcct gcaggcgggc ctgttcacag acaacctcgg
    10261 ccacgcgatg cgcgcatggg acgcgctggt cgaaggtggc gtgatcgtgg gcgacatccc
    10321 gagtttccgg gttgacaaca tgccgtacgg cggcgtcaag ctttccgggt tcgggcgtga
    10381 aggcatccgc tacgcgatcg acgacatgag cgaacgccgc ctgctggtga tccgtgatcc
    10441 gtaggacgga atcggcacag ccgcttccgc cgcacgtagc gggccgccga ttcggccgcc
    10501 ggattgaacc gcgatgccac gcccccatct cccgccgatg accgacaccg ccgaccgccg
    10561 gcgccccatc cgccagctcg ccgcgctggg cgcgctttgg cccttcatgc gccggcaccg
    10621 tgccctggcc ttcggctggc tgttcttcct ggcactgtcc tccgcggcca ccctgtcgct
    10681 gccggtcgcc gtgcgccaca tgatcgaccg cggtttcgcc agccacgatt cggcgctggt
    10741 caacgagacc tttctggtgc tgttcgtggt cgcgctggtg ctggccatcg cgaccgcggc
    10801 gcgttatttc tgcatcagcc tgctcggcga gcgcgcgatc gccgcgcttc gcgacctgct
    10861 gtattcgcgg atcatccgcc tcgacgtcgc gttctacgaa aagaaccggg tcggcgagct
    10921 gatctcgcgg ctcggcaccg acaccgaggt cgtgcagacg ctgatcggat ccagcatctc
    10981 gatcgcactg cgtagcgccg taaccgtgat cggggcagcc gcgctgatgg tctggaccag
    11041 cccccggctc gcgggcctga ccggcctcgt gatcccggtg gtgatcgtgc cgatcgcgct
    11101 gtacgggcgc aggctggcga aattgtcgcg gaccaaccag gatcgcatcg ccgacaccag
    11161 cgcgatcgcc aacgagacct tgaattcggt gtacgcggtg aaggcctatg cacgtgagcg
    11221 catcgaaagt tcacgctacg cggatgccgt ccagcgcgcg ctgcacgcgg cgcagcagcg
    11281 catcgccgca cgcgcggtcc tgaccggcgg cgtgatcgtg ctgatcttcg gtgcgatcac
    11341 cctggtactg tgggccggcg cgcgcgacgt gctcgcgggc agcatggacg caggcctgct
    11401 cagccagttc gtgctgtacg cggtgatggc agcaggttcc gtcggcgcgc tcggcggcgt
    11461 gtggggcgac gtgctgcgcg cggccggctc gatgggacgg atcagcgagc tgttggccga
    11521 gcacccgtcg atcgccgatc cggcacagcc cacgccgcta cccaagcccg cccggggcga
    11581 gatcgcgttc gaacacgtgg aattccacta tccgagccgg ccagacacct tggcgctgca
    11641 cgatttcagc ctgcggatcc ggcgcgggga aacggtcgcg ctggtcggcc cgtcgggcgc
    11701 cggcaagagc acggtgttca acctgctgct gcgtttccac gacccgcaac gcggacgcat
    11761 caccctggat ggcgtggacc tgcgctcgct ggaactggat gcactgcgcg gagccctggc
    11821 gctggtgcca caggaaaccg tgatattcgg cgccagcgcc gccgacaaca tccgcttcgg
    11881 gcgcgccgat gccgacgatg cctccgtgca tgcggcggcg cgcgccgccg aggccttcga
    11941 attcctcgac gggcagcccg agggattcga cacgcaactg ggcgaacgcg gagtccggct
    12001 gtcgggcggg cagcgccagc ggatcgcgat cgcgcgggcg atcctcaagg atgcgccggt
    12061 gctgctgctg gacgaggcca cttcgagcct ggatgcacaa tcggaagccg cgatccaggc
    12121 tgcgctcgag cgcgccggac gcggccgcac cacgctggtg atcgcccacc gcctggcgac
    12181 ggtattgcgc gccgaccgca tcgtcgtgat ggatgccggg cgcatcgtgg ccgacggcaa
    12241 ccacgagacg ctgctccgcg agggcgggct gtacgcggaa ctggcgcggc tgcagttcgc
    12301 ggcatagcgg cgccgcggga tgtccgacgt tttcccggct caggcggggg ccgccggagc
    12361 cgtggcgcct tcggcggtca cctcggcagc ctgcatgccc ttggggccct gcacgacctc
    12421 gaactggacg cgctggcctt ccttgaggct gcggaatccc tgcgcacgaa tcgccgaaaa
    12481 atgcacgaac acgtccgccc caccgccatc aggcgtgatg aacccgaaac ccttggcgtc
    12541 gttgaaccac ttcacggtac ccactgccat ttcgaaaacc cctttcgcaa cggtccatat
    12601 cacggcgctc tcccgccgtg gcgtgagtat caatcggacc catgccgtgt gcaagcaccc
    12661 cgatcgccgt cgactgtgac gcagccctcg gtggccggct gcggcatgca gggccgggct
    12721 cgagccggct tcgcccgccg cccgccgcga tcacctggcg gaggcttgcc caccttgccg
    12781 gccaaacctg gccgcgaatg caggtcagtc cgcgagcagc gcgcgcacga tcgccggaac
    12841 ccgcccggac gctgccgcga gatgtgcatg gatctcggcg agctcgatcg gcacgtcggg
    12901 ctggggaccg caaccggcgg cccagttcgc gaccaccgcc aagcaggcgt attccatgcc
    12961 gagctcacgt gccagagcgg cctccggcat tccggtcatt ccgacgaggt cgcaaccgtc
    13021 gcggcgcatc cgctcgatct cggcgcgggt ttccaggcgc ggcccctggg tcgccccgta
    13081 gcaaccgcca tccaccactt cgatcccggc ggccgctgca gcggacaaaa gcgcgcgccg
    13141 cagggaccgc gagtagggat cgccgaactc gacgtgcaac accgcctcgc catccgcgtc
    13201 ggagaaactg gcagccctgc cgcaggtgta gtcgatcaac tggtccggga tcgcaagcac
    13261 ccttggggcc atgtcgtcgc ggatgccgcc gacggcgtta atgccgacga cctgccggca
    13321 ccccagctcg tgcaaggcgg caatattcgc gcggtagttg atccggtgcg gcgcgatggc
    13381 gtgggtctcg ccgtgcctcg ccaggaatgc gaccgtccgc ccttccacct cgccggcggc
    13441 gatcgccgaa gagggggcgc cccagcgggt cgcgacctcg tggcgcaccg gatgtccgag
    13501 tccgggaaag gcgtatacgc cggtgccgcc gatgatggcg agatcgggaa gcgggggatg
    13561 cgatggcgta ttcatcggga gtacggcgat ggggaacggg tcgtgggcgc gcgctgatgc
    13621 accgcttgcg gacgtcgcaa aaccggcgtg tccgtgctgg cattcccgct acccatccgc
    13681 cacccccgct tcctcgaggg cgtagatgtc cggcaggttg cgcccctgcc cgtggtaatc
    13741 gagtccgtag ccgaacacgt agcgatcggg cacttccacc cctgcaaaat ccgcgacgag
    13801 atccggtacc cggcgatcgt ggcgtttgac gcacaacacc gcgatgagca cgcgcctggc
    13861 gcccgcggca aggcaatgcg cgcgcagtgc tgcgagcgtc aagccctcgt cgaggatgtc
    13921 gtcgaccagg atgaccgtgc gccctgcgag cgccacgcgc ggccggtgca gccattcgac
    13981 cccgcctccg acggtggctt cgccgtaacg agtcgcgtgc acgtaatcga actcgagatc
    14041 gcattcgatc gcgagcgcga gcggcgccgc gaagaacagg ccaccattca tcacggtgag
    14101 gaacaccgcc cgctcgccgt cgagctcggc cgtgatgccc gcgcccatcc gcgctatcgc
    14161 cgcctccaac gcatcccggc gatgcagctg ctcggcccgc ggcagcaccg cgccgagcgg
    14221 gtacgccggc gcgctcacgc gcgaggctcc gaatgggcgg cgagatcatc gagcgccgcg
    14281 acgtaacgcg cgtgctgggg gtcggcctgc aatgcacccc aacccgccgc gaccgaaccg
    14341 cacaaggcgg cgacgttttc ggccaaacaa ggcgtacggc cacgtaccga ttcgatcgca
    14401 tcgtcgacca tcgccggcgc acacaccaac accaggtcgc aaccggcatc gaggtgcgcg
    14461 gtgacccgtg cgccgatgcc gcctgcggct tccgccgcgg ccatcccgac gtcgtcgctg
    14521 aacaccacgc cgcggaatcc gaggtcgccg cgcaatactt cctcgatcca ttttcgggag
    14581 tagcctgcgg cgcgcgcatc caccgctggg taggtcacgt gcgcgaccat caccgcttcc
    14641 gcacccgccg cgaagccgtc gacgaacggc accaaatcgg tctcgcggat ctccgccagg
    14701 ggacgtgggt cgatcgcggc ttcgagatgc gtgtcctcgg ggatcgatcc gtggccggga
    14761 taatgcttca cggtggccgc catcccggcg agccgcatgc cgcgcacgta cgcggccgcg
    14821 agttcgccga ccaccgccgg gtcagggtcg aaggcacgat ccgcgatcac ccggttgccg
    14881 cgtgcgagat ccacgaccgg tgcaaacgag aggtcgatgt cgcaggcacg cacctccgtc
    14941 gccatcacga tcgcatgcat ttcggccatc gcgatcgcgg cgtcgcgatc ctggtggaac
    15001 aactcgccga tccgcgccag cggtgggagg cgcgtgaaac cgggcctgaa tcgttgcacc
    15061 ggaccgcctt cgtggtcgac gctcaacagg aagggatcgc cgcgcgcggc acggatcgaa
    15121 gcgaccaacc cggcgagctg gtcgtggtcc cggtaattgc gggtgaacag gatcgcgccg
    15181 ctgacctccg gcgcagccag ccgcacgcga tcgccgttcg tcagttcggt gccttcgatc
    15241 ccgatgatca gcatgcgcaa cgatcccgtc gt
//
LOCUS       DASSNH010000079         9662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1577529, whole genome shotgun sequence.
ACCESSION   DASSNH010000079 DASSNH010000000
VERSION     DASSNH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577529"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VFK00_02860"
     CDS             complement(<1..1206)
                     /locus_tag="VFK00_02860"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HET7591926.1"
                     /translation="MKRRTAPPPGTRVVASALDPLPFNLLCITLLCVVIAHAPHMPIW
                     YTAALAAILAARWWQRRRQRRRVGAWLRIVMLVAIPAVVIAVYGSPLGQQPGAAIVCG
                     LLVLKVLESESKRDARMAVGFACFILMSALLFDQALGFTIVVGLMLLPALATLRALEP
                     GLPHGAWLRAFRPGTVMLAASLPAALLGFLLIPRLATPLWGSPGNGAGTTGISDQMAP
                     GDLQHLLTDDAPAMRIGFDGAPPPEDQRYFRGVVLWDFDGRAWTPGVMAMRPWPPSPV
                     RTLGPATHYTVTLLPSHQHWLFALDVPIEAPEGANMGPDHTLWSVQPVDHAMRYWIAS
                     APGYRLAVEGLAPQVRAAALALPDGFDPRARALAASWRAHAGGNDAALVRDALELFHD
                     GGFVYDLDAP"
     gene            complement(1203..2165)
                     /locus_tag="VFK00_02865"
     CDS             complement(1203..2165)
                     /locus_tag="VFK00_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7591927.1"
                     /translation="MDAVARRRAPGIAARVRRWSRSLMRGRKPEPLPVRLDSHRIYIV
                     PTRAGFGFAILLVTMLVGALNYQNNAALLLTCMLGAALASSMLLTWRELHDLNLRSLH
                     ADHGFCDARLPLHLAFADDRHARHGLRISIDKLEQPCPLEPGNSVATVTMPTEHRGWM
                     PLPRLQVSSTLPFGLFRAWSWITPAQRVLVYPRILRDVRPPVRHDDPKERITGGDEFA
                     SLRDYRAGDPIRHVAWKASARHEHLLVREFDRAAPGQPVRLDWNALSGLDRETRISRL
                     TGWVCDAYAAAHPWILVLEDRGTMGPAADAAHYHRCLTALAELP"
     gene            complement(2170..3135)
                     /locus_tag="VFK00_02870"
     CDS             complement(2170..3135)
                     /locus_tag="VFK00_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7591928.1"
                     /translation="MDQNVAAAIEAIPAPADGLPRQRLDAALAQVNDVLVGKNRQVRM
                     AFACLLAGGHLLLEDVPGVGKTTLAHALAATLGLSFRRIQFTSDLLPSDIVGVSVFDR
                     DSGEFRFQRGPIFAQLLLADEINRATPKTQSALLESMAEGQVTVDGASRALQQPFFVI
                     ATQNPLDMAGTYPLPDSQLDRFMLRIALDYPDAEAERRLLTGEDRSRMVGRLAPRLAE
                     GDIHGLRLQARAATASPALLDYVQALLAASRRHHDIRTGLSPRAGLALLACARAWALL
                     GDRDFVLPEDVQAVFVGVAGHRLIPERNASRDALAQALLESVPVE"
     gene            complement(3205..3789)
                     /locus_tag="VFK00_02875"
     CDS             complement(3205..3789)
                     /locus_tag="VFK00_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HET7591929.1"
                     /translation="MSAPRIVLASTSRYRAELLRRLMDDFEQSAPEVDEQALPGETPA
                     TRAGRLAAAKASAVAAGAGEALVIGSDQVAALEGRVLHKPGTIENAREQLRASSGNVV
                     DFFTGLCVIASRSGQAWKALDHTRVAFRDLEDAEIDRYLARERPLDCAGSFKSEGAGI
                     ALFERIESEDPTALVGLPLIALSRLLRQAGAAVP"
     gene            complement(3786..4238)
                     /locus_tag="VFK00_02880"
     CDS             complement(3786..4238)
                     /locus_tag="VFK00_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591930.1"
                     /translation="MPTTLLAIVLPAGAALLACAAIIYVLLRQVRHQRALRTLYLEAD
                     GLEHDLKECRSRLARAHASMSMGPNQPAAGEAEARVAVDAALRELLAHRLWLRDEADG
                     ANQRELDAAVSAIGKARGALGRQLRELDSAQRALETAVREKIEDLSQQ"
     gene            4457..4999
                     /locus_tag="VFK00_02885"
     CDS             4457..4999
                     /locus_tag="VFK00_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET7591931.1"
                     /translation="MSASLPASVDAERMVAGRRSFEGTLPVAELPRLVEALADDRGDI
                     DYRLDFEQGKLGGPQLHVRLRAGLMLECQRTLETFEWPAAVDARLGLLADEAEAAALP
                     ADCDPLLLEGGALSPRRVIEDELLLALPLVPVKPGGEALQGQWSAAAEGRQDAAPEEP
                     VTHPFAELRDMLKTREQKHR"
     gene            5025..5219
                     /gene="rpmF"
                     /locus_tag="VFK00_02890"
     CDS             5025..5219
                     /gene="rpmF"
                     /locus_tag="VFK00_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7591932.1"
                     /translation="MAVAKSRKSPSRRGMRRSHDALDKVQLATDPTSGEIHRRHHITK
                     DGYYRGRKVIEDKQAIVDED"
     gene            5299..6282
                     /locus_tag="VFK00_02895"
     CDS             5299..6282
                     /locus_tag="VFK00_02895"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7591933.1"
                     /translation="MGSRTLTYARILATGSALPERVVTNDDLAKIVDTSDEWIRTRTG
                     IRERRIAADGETTGDLALRAAQQALADAGVKASDLDMVVLGTTTPDIIFPATACLIQN
                     RLGANGCMAFDVNAACSGFMYALGVANNFIRTGQVKRALVMGAETLSRMIDWSERETC
                     VLFGDGAGAVVLEASEEPGVIATCLHADGGYKHLLYNPVGVSAGFKDEKNHGVRITMS
                     GREVFKIAVKTLDALVDETLQAANMHADQIDWLIPHQANLRIIEATAKRLDMSMEQVI
                     VTVDKHANTSSGSVPLALDAAVRSGKIRRGQNVLLEAFGGGFTWASAMVRY"
     gene            6375..7352
                     /gene="fabD"
                     /locus_tag="VFK00_02900"
     CDS             6375..7352
                     /gene="fabD"
                     /locus_tag="VFK00_02900"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7591934.1"
                     /translation="MNTSVSNPAPSSPLPALAFVFPGQGSQSVGMLAELAAEFPEVRA
                     TFDEASEGAGIDLWALSQNGPEDELGRTENTQPALLAASVATWRVWLAQGGPVPSQMA
                     GHSLGEYSALVCANALQLREAAALVAERGRLMQAAVPAGTGSMAAVLGADDELILEVC
                     REVSGAEIVTPANFNSPGQLVIAGHAGAVERATQRLAERGVRKVIKLAVSVPSHTPLM
                     RDAAHQLAAKMAELPWQVPIIPVLQNADAKGHVGIEFIRAALERQLYMPVLWTDIVQS
                     LVRSGVTHILECGPGKVLSGLCRRIDKSLDARSLGTPTELRAALAELKP"
     gene            7349..8092
                     /gene="fabG"
                     /locus_tag="VFK00_02905"
     CDS             7349..8092
                     /gene="fabG"
                     /locus_tag="VFK00_02905"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HET7591935.1"
                     /translation="MSGLLEGEVALVTGASRGIGAAIADELAAGGAKVIGTATSESGA
                     QAVTQRLSGSGGEGRVLDVTDGKAVEALIDAIVKDHGGLSILVNNAGITRDQLLMRMK
                     DEDWQAILDTNLTSVYRTSKAVMRTMMKARKGRIINIASVVGVTGNPGQTNYAAAKAG
                     IIAFSKSLAREVGSRGITVNVVAPGFIDTDMTRALGEDQRKALEASIALGRLGAATDI
                     AHAVAFLASPAANYITGETLHVNGGMYMA"
     gene            complement(8208..8951)
                     /locus_tag="VFK00_02910"
     CDS             complement(8208..8951)
                     /locus_tag="VFK00_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET7591936.1"
                     /translation="MSLDWLHPLSWPAIQAAVVGTIGLLLLALRRYRTGVFLAVLAAA
                     WIWLCATPSFATWLHQGLQDGYPPRAAAAYPEAQAIVVLGGSDLLRSSRPGRANDGSV
                     SPSRVGFGLALYQAERAPIVLLSGGGGAAIKMARTLSREGVPAGALQTETRSGNTHEN
                     ALYSAAILQREHKQRILLVTSAMDMPRASASFERQGLTVIPAPVPYDPDWLGVTQHDW
                     WPRHRALYLTSRCLHEIVGLWAYKLLGWA"
     gene            9339..9578
                     /gene="acpP"
                     /locus_tag="VFK00_02915"
     CDS             9339..9578
                     /gene="acpP"
                     /locus_tag="VFK00_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7591937.1"
                     /translation="MSSIEERVKKIVVDQLGVKEEDVTPNASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAVDYVKAHVKS"
BASE COUNT         1611 a         3176 c         3405 g         1470 t
ORIGIN      
        1 cggtgcatcg aggtcataga cgaatccgcc gtcgtggaac agctccaggg cgtcgcgcac
       61 gagcgccgcg tcgttgccgc cggcgtgcgc gcgccagctc gccgccagcg cacgcgcgcg
      121 agggtcgaat ccgtccggca gggcgagggc tgcggcgcgc acctgcgggg caagcccttc
      181 gacggcaagc cggtacccgg gtgccgaggc gatccagtag cgcatcgcgt ggtcgacggg
      241 ctgcaccgac cacagcgtgt ggtccgggcc catgttcgcg ccctccgggg cttcgatcgg
      301 cacgtccagt gcgaacagcc agtgctggtg gctcgggagc agggtgacgg tgtagtgcgt
      361 cgcgggcccg agggtgcgga cgggcgaagg tggccaaggc cgcatggcca tcacgcccgg
      421 ggtccaggcg cgtccatcga aatcccacag caccacgccg cggaaatagc gctggtcctc
      481 cggtggcggc gcgccgtcga agccgatgcg cattgcgggc gcgtcgtcgg tcaggaggtg
      541 ctgcaggtcg ccgggcgcca tctgatcgga gatgccggtg gtccccgcgc cgttcccggg
      601 cgagccccac agcggcgttg ccaggcgcgg gatcaacagg aatccgagca gcgccgccgg
      661 caggctggcg gccagcatca cggttccggg ccggaacgcg cgcagccagg cgccatgcgg
      721 caggccgggt tcgagcgcgc gcaaggtcgc gagtgccggc aggagcatca gtccgaccac
      781 gatggtgaag cccagtgcct ggtcgaacag caacgcgctc atcaggatga agcaggcgaa
      841 accgaccgcc atccgtgcgt cgcgtttgga ttcgctttcc agcaccttca acaccagcag
      901 cccgcaaacg atggcggcgc ccggctgctg gccgagcggc gagccgtaca ccgcgatcac
      961 caccgccggg atcgccacca gcatcacgat cctgagccac gcgccgacgc gccggcgctg
     1021 cctgcggcgt tgccaccagc gcgccgcgag gatcgccgcc agtgccgcgg tgtaccagat
     1081 cggcatgtgg ggcgcatgcg cgatgaccac gcacagcaag gtgatgcaca gcaggttgaa
     1141 cgggagcggg tcgagcgcgg aggcgaccac gcgcgttccg ggcggcggcg cggtgcggcg
     1201 cttcacggca gctccgcgag tgccgtcagg cagcggtgat agtgcgcggc gtcggccgcc
     1261 ggccccatcg tgccgcggtc ttcgaggacc aggatccacg gatgcgccgc ggcgtaggcg
     1321 tcgcaaaccc agccggtgag ccgcgagatc cgcgtttcgc ggtcgaggcc cgacaatgcg
     1381 ttccagtcca ggcgcaccgg ttggcccggc gccgcgcggt cgaactcgcg caccagcagg
     1441 tgctcgtggc gagcgctggc tttccaggcg acgtggcgga tcggatcgcc ggcacggtaa
     1501 tcgcggagac tcgcgaactc gtcgccgccg gtgatgcgtt ccttcggatc gtcgtggcgc
     1561 accggcgggc gcacgtcgcg caggatccgc ggatacacca gcacgcgttg cgccggggtg
     1621 atccagctcc acgcgcgaaa caggccgaac ggcagggtgc tcgacacctg caggcgcggc
     1681 aacggcatcc agccgcggtg ctccgtcggc atggtcaccg tggcgacgct gttgccgggt
     1741 tccagggggc agggctgctc cagcttgtcg atggagattc gcaatccgtg gcgggcgtgg
     1801 cggtcgtcgg cgaaggcgag gtgcaacggc agccgggcgt cgcagaagcc gtggtccgcg
     1861 tgcagcgacc gcaggttgag gtcgtgcagt tccctccagg tgagcagcat gctgctcgcg
     1921 agcgccgccc ccagcatgca ggtcaggagc agcgccgcgt tgttctggta gttcagcgcg
     1981 ccgaccagca tcgtgaccag caggatcgcg aagccgaagc cggcgcgcgt ggggacgata
     2041 tagatccggt ggctgtcgag tcgcaccggc agcggttcgg gcttgcgccc gcgcatcagg
     2101 ctgcgcgacc accggcgtac ccgggcggcg atgccgggcg ctcggcggcg ggcgacggca
     2161 tccatgtcgc tactcgaccg gcacggattc cagcagcgcc tgggccagcg cgtcgcgcga
     2221 ggcgttccgc tccggtatca ggcggtggcc ggcgacgccc acgaacaccg cctgcacgtc
     2281 ttccggcagc acgaagtcac gatcaccgag caacgcccag gcgcgggcgc aggccagcag
     2341 cgcgagcccc gcgcgaggcg acagtccggt gcggatgtcg tggtgcctgc ggctggcggc
     2401 gagcagggcc tgcacgtagt cgagcagggc aggactcgcc gtcgccgcac gcgcctgcag
     2461 gcgcaggcca tggatgtcgc cttcggccag ccgcggcgcg aggcgcccga ccattcgcga
     2521 gcggtcctcg ccggtcagca gccggcgttc ggcttcggcg tcgggatagt ccagcgcgat
     2581 ccggagcatg aaacggtcga gctgcgagtc gggcagggga taggtgcccg ccatgtccag
     2641 cggattctgg gtggcgatca cgaagaatgg ctgctgcaag gcacggctcg cgccgtccac
     2701 ggtcacctgg ccttcggcca tcgactccag cagcgcgctc tgggtcttcg gcgtcgcgcg
     2761 gttgatctcg tcggccagca ggagctgcgc gaagatcggc ccgcgctgga accggaactc
     2821 gccgctgtcg cgatcgaaca cgctgacccc gacgatgtcg ctgggcagaa ggtcggaagt
     2881 gaactggatc cgccggaagc tgagcccgag ggtcgccgcc agcgcgtgcg caagcgtggt
     2941 cttgccgacg ccgggtacgt cttccagcag caggtgcccg ccggccagca ggcatgcgaa
     3001 cgccatccgg acctgccggt tcttgccgac cagcacgtcg ttgacctgcg ccagcgcggc
     3061 gtctaggcgc tggcgcggca aaccgtccgc gggtgcgggg atggcttcaa ttgccgcagc
     3121 gacgttctgg tccatgtgcc gaaagcctcg ggtgcttgcg cagtgtacga tgccgaatgt
     3181 cgccaaaatg cgaaccctgg cgcttcatgg aacggcggct ccggcctgcc gcagcaggcg
     3241 cgacagggcg atcagcggca aaccgaccag cgctgtcggg tcctcgcttt cgatgcgttc
     3301 gaacagggcg atccccgcgc cttccgattt gaagctgccg gcgcaatcga gcggtcgttc
     3361 gcgggcgaga tagcggtcga tctcggcgtc ctcgaggtcg cggaaggcga cccgggtatg
     3421 gtcgagcgcc ttccaggctt gcccgctcct gctggcgatc acgcacaggc ccgtgaaaaa
     3481 atccacgaca tttccgctac ttgcgcgcag ttgttcgcgt gcattttcga tcgtcccggg
     3541 cttgtggagg acgcgccctt cgagcgcggc gacctggtcc gaaccgatca ccagtgcttc
     3601 gccagctccg gccgcgaccg cggaagcctt ggctgccgcc agccggcctg cccgcgtggc
     3661 cggggtctcc ccgggcaacg cctgttcatc gacctccggt gccgactgct cgaaatcgtc
     3721 catcagccgt cgcaacagct cggcccgata gcgcgaagtc gatgccagca cgatccgcgg
     3781 ggcactcatt gctgggaaag atcctcgatc ttctcgcgca ccgcggtttc cagggcgcgc
     3841 tgcgcggaat ccagctcgcg aagctggcgc cccaacgctc cgcgcgcctt gccgatcgcg
     3901 ctgaccgcgg cgtccagctc gcgctggttg gcgccgtcgg cctcgtcgcg cagccacagg
     3961 cggtgcgcca gcagttcgcg aagcgcggca tcgaccgcga cccgggcctc ggcctcgccg
     4021 gccgccggct ggttcggccc catcgacatc gaggcatgtg cccgcgcgag tcgcgagcgg
     4081 cattccttga gatcgtgctc gagaccgtcc gcctccaggt acagggtgcg caaggcgcgc
     4141 tggtggcgta cctggcgaag cagcacgtaa atgatcgccg cgcaagcgag caacgccgcc
     4201 cccgcgggca ggacgatggc gagcagcgtg gtgggcaagg gaatcaagtg cgcggggggc
     4261 gtcgtggatg gaatagctgc aagcatgata cgggccaaat ggatggcagg ggagccgctc
     4321 gccgtgatgc cgaaggacaa tcgggaaaac ggccgctcac cgcgctcgag caacccgaaa
     4381 cattccgttc cacggttccc gtttcgccgc gctcgtggtt gactttgtgc actgcggaat
     4441 ctagcattcc gcgcttatgt ccgcgtccct gccagcgagt gtggacgccg aacgcatggt
     4501 ggccgggcgg cggtctttcg agggtacctt gccggttgcg gaattgccgc gactggtgga
     4561 ggcgctggcg gacgatcgcg gcgacatcga ctaccggttg gacttcgagc agggcaaact
     4621 gggcggtccg caattgcacg tgcggctgcg ggcgggcctg atgctggaat gccagcgtac
     4681 gctcgagacg ttcgaatggc cggccgcggt ggatgcacgg ctgggactgc tggcggacga
     4741 ggccgaagcc gcggcgttgc ccgccgattg cgatccgttg ttgctggaag gcggcgcgtt
     4801 gtcgccgcgg cgggtcatcg aggatgaatt gttgctggcg ttgccgctgg tgcccgtcaa
     4861 gcccggcggc gaagcattgc agggccaatg gagtgccgcc gcggaaggcc ggcaggacgc
     4921 ggcgccggaa gagcctgtca cgcacccgtt cgcggaattg cgcgacatgc tgaagacccg
     4981 cgaacagaaa caccgttgaa gtcgaagcat tcaggagagc catcatggcc gttgccaaga
     5041 gccgcaaatc accgtcccgt cgcggcatgc gccgttcgca cgacgcactg gacaaggtgc
     5101 agctcgcgac cgaccccacg tcgggcgaga tccatcgtcg ccaccacatc accaaggacg
     5161 gctactaccg tggccgcaag gtgatcgagg acaagcaggc gatcgtcgac gaggactgag
     5221 gtccttgtgc acgtggctgc aaaagggcgc gcggcgcgat caggcgccgc cgcgtttttt
     5281 tgcgcgcgca atttttgaat ggggagccgt accttgacct acgcccgcat cctcgcgacc
     5341 ggcagcgcct tgcccgagcg cgtggtcacc aatgacgatc tcgcgaagat cgtcgacacc
     5401 agcgacgagt ggatccgcac ccgcaccggc atccgcgaac gccgcatcgc cgccgacggc
     5461 gagaccacgg gcgacctggc cttgcgtgcg gcgcaacagg cgctggccga cgccggcgtc
     5521 aaggcatcgg acctcgacat ggtggtgctc ggcaccacca ccccggacat catcttcccg
     5581 gcgaccgcat gcctgatcca gaaccggctc ggcgcgaacg gctgcatggc gttcgacgtg
     5641 aacgccgcgt gctccggctt catgtacgcg ctcggggtcg ccaacaattt cattcgcacg
     5701 ggacaggtga agcgcgcgct ggtgatgggc gcggaaacgc tgagccgcat gatcgactgg
     5761 agcgagcgcg agacctgcgt gctgttcggc gatggcgccg gtgcggtggt gctggaagct
     5821 tccgaggagc cgggcgtgat cgccacctgc ctgcacgccg acggcggcta caagcacctc
     5881 ctgtacaacc cggtgggcgt atccgccggt ttcaaggacg agaagaacca cggcgtcagg
     5941 atcaccatga gcggccgcga ggtcttcaag atcgcggtga agaccctcga tgcgctggtg
     6001 gacgaaaccc tgcaggcggc gaacatgcac gccgaccaga tcgactggct gatcccgcac
     6061 caggccaacc tcaggatcat cgaagccacc gccaagcgcc tcgacatgtc gatggagcag
     6121 gtgatcgtca ccgtcgacaa gcacgccaac acatcgtccg gatcggtgcc gctggcgctg
     6181 gacgctgccg tgcgctcggg caagatcagg cgcggacaga atgtgttgct ggaagccttc
     6241 ggcggcggct tcacctgggc ctcggcgatg gtgaggtatt gaaaggacag ggaacaggga
     6301 acagggagag cgacccgcgg cgcttcgcgg ctcatccggt accagcttct tttgaaagtc
     6361 agagctacca cgcaatgaac acgtcggttt ccaaccctgc tccgtcctcc ccgctccccg
     6421 ctctggcctt cgtcttcccc ggccagggtt cgcagtcggt cggcatgctg gccgagctcg
     6481 ctgccgagtt cccggaagtt cgcgcgacct tcgacgaggc atccgagggc gcgggcatcg
     6541 acctttgggc gttgtcgcag aacggtccgg aagacgaact cggcaggacc gaaaacaccc
     6601 agcccgcgct gctggcggcg agcgtcgcca cctggcgggt gtggttggcc cagggcggtc
     6661 cggtacccag ccagatggcc ggccacagcc tcggcgaata ctcggcgctg gtgtgcgcga
     6721 acgcgttgca attgcgcgaa gcggcggcgc tggtcgccga gcggggccgg ctgatgcagg
     6781 cggcggttcc cgccggcacg ggttccatgg cagcggtgct gggcgccgac gacgaactga
     6841 tcctggaagt gtgccgggaa gtttccggtg cggaaatcgt caccccggcg aatttcaaca
     6901 gcccggggca gttggtgatc gcggggcatg cgggcgccgt tgaacgcgcc acccagcggc
     6961 tggccgagcg cggcgtgcgc aaggtcatca agctcgccgt gtcggtgcct tcgcacacgc
     7021 cgttgatgcg cgatgccgcg caccagctcg cggcgaaaat ggccgaactc ccctggcagg
     7081 tgccaatcat ccccgtcctg cagaacgccg acgccaaggg ccacgtcggc atcgagttca
     7141 tccgcgcggc gctggagcgc cagttgtaca tgccggtgtt gtggaccgac atcgtgcagt
     7201 cgctggtccg cagcggcgtg acgcacatcc tcgaatgcgg gcctggcaag gtgctttcgg
     7261 ggttgtgcag gcgcatcgac aaatcactcg acgcccggtc gctcggcacg cccaccgagc
     7321 tgcgcgcggc gcttgcggag ttgaagccat gagcggattg ctggaaggcg aagtcgcgct
     7381 ggtgacgggc gcctcgcgcg gcatcggtgc cgcgatcgcg gacgagctgg ccgcaggggg
     7441 cgctaaggtg atcggcaccg cgacgtccga atcaggcgcg caggcggtca cgcagcggct
     7501 ctcggggtcg ggtggcgaag ggcgcgtgct cgacgtgacc gacggcaagg cggtggaagc
     7561 cttgatcgat gccatcgtca aggaccacgg cggcctctcg atcctggtga acaatgccgg
     7621 catcacgcgc gaccagttgc tgatgcgcat gaaggacgag gactggcagg ccatcctcga
     7681 caccaatctc acctcggtct atcgcacctc gaaggcggtg atgcgcacga tgatgaaggc
     7741 ccgcaagggg cgcatcatca acatcgcctc ggtggtcggc gtcaccggca atccggggca
     7801 gaccaactac gccgccgcca aggcggggat catcgcgttc tccaaatcac tggcacgcga
     7861 agtcggatcg cgcggcatca ccgtcaacgt ggtcgcgccg ggattcatcg acacggacat
     7921 gacccgcgcg ctcggcgaag accagcgcaa ggcgctcgag gcctccatcg cgctcggccg
     7981 cctcggtgca gccaccgaca ttgcgcacgc ggtggccttc ctggcgtcgc ctgccgcgaa
     8041 ttacattacc ggcgagacct tgcacgtcaa cggcggcatg tacatggctt agcttttgcg
     8101 atcgcccctg atcgcggctt cgaccgatca gccagcgagc gggactgtct gcacgtcttg
     8161 aagtccagaa cggccattca tggcctgact tttcaagaga gccggattca cgcccagccg
     8221 agcagcttgt aggcccacaa ccccacgatc tcgtgcaggc agcggctggt gagatacagc
     8281 gcgcgatgcc gcggccacca gtcgtgctgc gttacgccca gccaatccgg atcgtagggc
     8341 accggtgccg gaatcaccgt gaggccttgg cgttcgaagc tggcggaggc gcgcggcatg
     8401 tccatcgccg aggtcaccag caggatgcgc tgcttgtgct cgcgttgcag gatcgccgcg
     8461 gagtacagcg cgttctcgtg ggtgttgccg ctgcgcgttt cggtttgcag cgcgcctgcc
     8521 ggcacgcctt cgcggctgag cgtccgggcc atcttgatcg ctgcgccacc cccgcccgag
     8581 agaagcacga tgggcgcgcg ctccgcctgg tacaacgcga gcccgaagcc gacccggctt
     8641 ggcgacacgg acccgtcgtt cgcccgtccg ggtcgggacg atcgcaacaa gtcggagccg
     8701 ccaagcacga cgatcgcctg cgcttccgga taggccgccg cggcccgcgg cggatacccg
     8761 tcttgcaggc cctggtgcag ccaggttgcg aacgaggggg tggcgcacag ccagatccac
     8821 gctgctgcca gcactgccag gaaaacgccg gtgcgatagc gcctcagggc gagcagcagc
     8881 aagccgatgg tgccgacgac ggcggcctgg atcgcgggcc aacttagcgg atgcagccag
     8941 tcaaggctca cggcaagggc gtttccgatt ccgggactgt gtgcaccgat ggttcccgtg
     9001 gatagctggg tggatgggag cgaggttagc agcgggcgag cgttgcctgc acggctgcat
     9061 cgccggatcc gcggcgggcg tgaattccgg ggcgtcggcg cgaagatggg cggccatcgg
     9121 gcgtggttct attgaccgcc cgaaccgggt aatatggcgt ctttcgtgcc gcggccggag
     9181 cgtggaaggc cgtcgcggaa ttccacgtcg aatgtcgtgg tccgactcga acttgtaccc
     9241 tgcggatcca gaaggtcacg cccagaaggt cacgcatggg gtagtgggga ggggcagggc
     9301 ccgtcctcga atgcatttgt cccgggaggc actgtcacat gagcagcatc gaagagcgcg
     9361 tcaagaagat cgtggtcgat cagttggggg tcaaggaaga agacgtcacg ccaaacgctt
     9421 cgttcgttga cgatctgggc gcggactcgc tggacacggt ggaactggtg atggccctcg
     9481 aagaggaatt cgaaaccgaa atccccgacg aggaagccga gaagatcacc accgtgcagc
     9541 aggcggtgga ctacgtcaag gcgcacgtca agtcctgacc gcgattgccg catgcctcgc
     9601 ggcagcgcaa tcgacgtcat cgcggcacat cgggagctgc gccttggtgc gcagctttcg
     9661 ct
//
LOCUS       DASSNH010000080         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_670102, whole genome shotgun sequence.
ACCESSION   DASSNH010000080 DASSNH010000000
VERSION     DASSNH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670102"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFK00_02920"
     CDS             complement(<1..822)
                     /locus_tag="VFK00_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7591938.1"
                     /translation="MLRIRLLPVLVAGTIFTSSALAAPPPANTHPELHEIAGAVKASN
                     LHATDVALVGFGTRHTLSTTSSKTRGIGPARRWVAGQFETISKGCDGCLEIVTPSQVF
                     TGSRMPKAGAEVMDVVAIQRGTTDPDRVIVMTAHLDSRRSDVMDATGDSPGANDDAAG
                     VAALIETARVLSKYKFPATLVYSADSGEEQGLYGGKIIAQYAVDHGWKVEANLNNDMV
                     GNSHGGNGAYDPHTIRVFSEGTKSNETLEQAAYRRYHGGGVDSPSRNIARYMAALA"
     gene            complement(890..2464)
                     /locus_tag="VFK00_02925"
     CDS             complement(890..2464)
                     /locus_tag="VFK00_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7591939.1"
                     /translation="MNTGTTSDPSWRWLALWCALLLPLAILFPPIPIDETRYLTAAWE
                     MFNSGQWLVPTVNGAWYSDKSPLLFWLIAGGWKIFGVHTWVARVEALIVASCMLLALR
                     RLAARLGMDARGAGDAMWMLAGCLGFTVFSTAIMFDLLLSLCVLGALHALLDLDAGRW
                     TRGIVALGLTIGLGILAKGPVMLLHIVAVALLAPLWSDTARLHKARWYFALLGGFLIG
                     VAIALCWLLPAAWYAGPTYWQPLLDKVTGRIAKSFAHRRAWWWYLPLVPVLLLPWLLT
                     LRAPATAWKNLWRGRFGHFLGCWWVVPFVAFCAISGKQIHYLLPLLPAWALAGAWLLQ
                     QPGGRLRPLLFVLLALLAAALIAVAPWHAAKSFGYPPQPAIAGFALIGLIVAAGAWRG
                     WACDARGMALSGTALVSAAMLAGALALLPSLNVEPEAAFVKQALHEHVPIATVEWNNG
                     LLGYTGRLHQPVPWIPRDQIRAWSRAHPDGVLLTSEAHDELKSATPFKAWPYFLSGNH
                     RIAAWHAAEVLDDSPH"
     gene            complement(2645..>2751)
                     /locus_tag="VFK00_02930"
     CDS             complement(2645..>2751)
                     /locus_tag="VFK00_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET7591940.1"
                     /translation="ILAVEEEHAEDMSTLLEQLGHKGEPAKARPSRGA"
BASE COUNT          530 a          938 c          879 g          404 t
ORIGIN      
        1 ggccagcgcc gccatgtaac gcgcgatgtt gcgcgagggc gaatccacgc cgccgccgtg
       61 gtagcggcgg tacgcggcct gctccagcgt ttcgttgctc ttggtgcctt cggagaacac
      121 gcggatcgtg tgcggatcgt acgcgccgtt gccgccgtgc gagttgccga ccatgtcgtt
      181 gttgagattg gcctcgacct tccagccatg atcgacggcg tactgggcga tgatcttgcc
      241 gccgtagagg ccctgttcct cgccggaatc ggccgagtac accagcgtcg ccgggaactt
      301 gtacttcgag agcacccgcg cggtttcgat cagcgcggcg acgcctgcgg cgtcgtcatt
      361 cgcgcccggc gaatcgccgg tcgcgtccat cacgtcgctg cggcgggaat cgaggtgcgc
      421 ggtcatcacg atcacgcgat cgggatcggt ggtgccgcgc tggatcgcca ccacgtccat
      481 cacctcggcg ccagccttgg gcatccggga accggtgaat acctgcgatg gcgtgacgat
      541 ttcgaggcag ccatcgcagc ccttggagat cgtttcgaac tgccccgcca cccagcgccg
      601 cgccggaccg atgccgcggg ttttcgagga ggtcgtcgaa agcgtgtggc gggtgccgaa
      661 accgaccagg gcgacatcgg tggcgtgcag gttggatgcc ttcaccgcgc cggcgatttc
      721 gtgcaattcg ggatgggtat tggcaggcgg tggcgccgcg agcgccgaac tggtgaagat
      781 cgtcccggca accaagacgg gcagcaaacg aatgcgcaac atggcaatcc ctcgtcaacg
      841 gtaccgcccg agggtagtcc gtcggcttcg tgattgcatg tggccaaggt cagtgcgggg
      901 aatcgtcaag cacctccgcc gcgtgccagg ccgcgatgcg atggttgccg gacaggaaat
      961 acggccaggc cttgaacggt gtggcggact tcagttcgtc atgggcttcc gaggtcaaca
     1021 ggacgccgtc aggatgcgcg cgagaccagg cacgaatctg gtcccgcgga atccacggca
     1081 ccggttgatg cagacgaccg gtgtaaccca gcaagccgtt gttccattcg accgtggcaa
     1141 tcgggacatg ctcgtgcagc gcttgtttca cgaacgcagc ctcgggttcg acattcaacg
     1201 acggcaataa cgccagcgca ccagccagca tcgcggccga aaccagggcg gtgccagaca
     1261 aagccatgcc gcgcgcatcg cacgcccagc cgcgccacgc gcctgccgcg acgatcaacc
     1321 cgatcaatgc aaaccccgct atcgcaggtt gtggtggata cccaaaggat ttcgccgcgt
     1381 gccacggcgc cactgcgatg agtgcggcgg cgagcagcgc aagcaggaca aacagcaacg
     1441 ggcgaaggcg accgccgggc tgctgcagca accacgcgcc ggccagcgcc cacgccggca
     1501 gcagcggcag aagatagtgg atctgcttgc cgctgatcgc acagaacgcc acgaacggca
     1561 ccacccacca gcaccccagg aaatggccga agcggccgcg ccacaaattt ttccaggccg
     1621 ttgccggcgc gcgcagggtc aacagccaag gcagcaacag caccgggacc agcggcaggt
     1681 accaccacca cgcccgccgg tgcgcgaagc ttttggcgat gcggccggtg accttgtcca
     1741 gcaacggttg ccagtaggtc ggccccgcgt accaggccgc cggcagcagc cagcacagcg
     1801 cgatcgcgac gccgatcagg aaaccgccga gcagcgcgaa gtaccagcgc gccttgtgca
     1861 accgcgcggt atcgctccac agtggcgcca gcagcgcgac cgccacgatg tgcagcagca
     1921 tcaccggacc cttggcaagg atgcccagcc cgatcgtcag ccccagcgca acgatgccgc
     1981 gcgtccaacg gcctgcatcc aggtcgagca gtgcgtgcag cgcgccaagt acgcacagcg
     2041 acaacagcag atcgaacatg atcgccgtgc tgaacaccgt gaaaccgagg cagccggcga
     2101 gcatccacat ggcgtcgcct gcgccgcgcg catccatccc gaggcgcgcg gccagccgcc
     2161 gcagcgccag caacatgcac gaggcaacga tcaacgcctc gacgcgcgcg acccaggtgt
     2221 gcacgccgaa aatcttccag ccacccgcga tcagccagaa cagcagcggc gacttgtcgg
     2281 aataccacgc accgttgacg gtcggcacca gccactgacc ggagttgaac atctcccacg
     2341 cggcggtcag ataacgcgtt tcgtcgatcg gaatgggcgg aaacaggatc gccagcggca
     2401 gcagcaatgc acaccaaagt gccagccagc gccacgatgg atcagaagtc gttccggtat
     2461 tcatgcgctg cgcagcataa cggacagtgc cgcgctcgtg cgcccctcac ggatcaacgc
     2521 ggtccggcag gcacgccggg gcgcgacccc gtcgccacat cgtcaaggca gcgaaacaac
     2581 gccggacgcg ggtcaacggc ttgctgatcc gcgctggccg gcatcgcatg catccgcttg
     2641 ccgctcaggc accccttgac ggtcgcgcct tggcgggctc gcccttgtgg ccgagctgtt
     2701 ccagcagcgt gctcatgtct tcggcgtgct cttcttccac cgccaggatt t
//
LOCUS       DASSNH010000081         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_828129, whole genome shotgun sequence.
ACCESSION   DASSNH010000081 DASSNH010000000
VERSION     DASSNH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828129"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VFK00_02935"
     CDS             complement(<1..1237)
                     /locus_tag="VFK00_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916210.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET7591941.1"
                     /translation="MNPQPNTLLVNPTITSRHSARFPLSLLNLSAALDESGSSRIVDG
                     NLDRNVVDATLRALERERFDALGISVMGGPHVAPAVAISKAVHERHPRLPIIWGGYFP
                     TLHTDAVLAEPYVDYAVRGQGERTLPELVASLCGHGPGLHEIAGLSWKGAEGVIHNPA
                     RPFSPNRLTPLLPYDKLGDPRRYLARTYLGRHTVAHQAALGCRFRCTFCGVAAMFGGA
                     TALPPLERFEQELHYLKHTIGADSIQYFDHNFFDREADMLPLLEVMARFELPWWCFAR
                     TDALLNLSGQAWKLVQKSRLRMAFMGAETPDRQMLKTIRKGTRPDQALAVAELCGRMG
                     VIPELSFMVAPPEDTEEETERTFEFIRQLKRVNPACEIIVYVYTPLPERSMHAKDRGK
                     GRAPALLDARGEPVVFPATA"
     gene            complement(1234..1935)
                     /locus_tag="VFK00_02940"
     CDS             complement(1234..1935)
                     /locus_tag="VFK00_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7591942.1"
                     /translation="MSRIEMLEPRDAYALWAASYPPHAHNPVMRAEERAMQALMPANL
                     QGQAVLDAGCGSGRYMLHALRRDAKHVTGVDLSIEMLARAGAELAAYRDAGQVELVQG
                     DLLKLPLPDAAADLTICGLTIGHLDRLHPALSELRRVTRPGGAILCSDVHPTGHALGW
                     ARDFKSDGRHLAARHTPHFYSAWHAACAALHLDIARVLEPMLDPADIPIDAHFDPLAL
                     EVPVALVFLLRRRLS"
     gene            complement(1987..2920)
                     /locus_tag="VFK00_02945"
                     /pseudo
     CDS             complement(1987..2920)
                     /locus_tag="VFK00_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT          463 a         1001 c         1001 g          455 t
ORIGIN      
        1 ccgcggtcgc gggaaacacg accggttcgc cgcgggcgtc gagcaacgcc ggcgcccgcc
       61 ccttgccgcg atccttcgca tgcatgctgc gttcgggaag cggcgtgtag acgtagacga
      121 tgatttcgca ggccggattg acgcgtttca actggcggat gaactcgaag gtgcgttcgg
      181 tttcctcttc ggtgtcttcc ggcggtgcga ccatgaacga cagctccggg atcacgccca
      241 tccggccgca cagctcggcg accgcgagcg cctggtccgg ccgcgttccc ttgcggattg
      301 tcttgagcat ttgtcggtcc ggggtttccg cgcccatgaa cgccatgcgc aaccggctct
      361 tctgcaccag tttccaagcc tgcccggaca ggttcagcaa cgcgtcggtg cgcgcgaagc
      421 accaccaggg cagctcgaag cgggccatga cttccagcag cgggagcatg tcggcttcgc
      481 ggtcgaagaa gttgtggtcg aagtactgga tcgaatcggc cccgatcgtg tgtttcaggt
      541 agtgcaattc ctgctcgaac cgttcgagcg gcggcagcgc ggtcgcgccg ccgaacatcg
      601 cggccacgcc gcagaacgtg cagcggaagc ggcagccgag cgccgcctgg tgcgcgacgg
      661 tgtgccgccc gaggtaggtg cgcgcaaggt aacggcgcgg gtcgccgagc ttgtcgtacg
      721 gcagcaacgg cgtgagccga ttgggcgaaa acggccgcgc cggattgtgg atgacgcctt
      781 cggcaccctt ccatgacagg cccgcgattt catgcaaccc cggcccgtgc ccgcacaacg
      841 atgcgacgag ttccggcaat gtccgttcgc cctgtccgcg gaccgcgtag tcgacgtagg
      901 gctcggccag cacggcgtcg gtgtggagcg tcggaaaata gccgccccag atgatcggca
      961 agcggggatg gcgctcgtgg acggccttgg agatcgcgac tgccggtgcg acgtgcggcc
     1021 cgcccatcac gctgatgccg agcgcgtcga agcgctcgcg ctccagcgcg cgcaaggttg
     1081 catcgaccac gttgcgatcc aggttgccgt ccacgatccg gctgcttccg ctttcatcca
     1141 gcgccgcgga caggttcagc agcgacaacg ggaaccgtgc actgtggcgc gaggtgatgg
     1201 tcgggttgac cagaagcgtg ttgggctgtg ggttcatgaa agccggcgcc gcagcaggaa
     1261 caccagggcc accggcacct cgagcgccag cggatcgaaa tgggcatcga tcgggatatc
     1321 cgcgggatcg agcatgggtt ccagcacacg cgcgatgtcc aggtgcaagg ccgcgcaggc
     1381 ggcgtgccat gcgctgtaga aatgcggcgt atgcctcgcg gccaggtgcc gcccgtcgga
     1441 tttgaaatcc cgcgcccagc cgagcgcgtg tccggtcggg tgcacgtcgc tgcacaagat
     1501 cgcgccgccc ggccgtgtga cccgccgcaa ctccgacagc gccgggtgca gccggtccag
     1561 gtgcccgatc gtcaagccgc agatggtcag gtccgcggca gcatccggca gcggcagctt
     1621 gagcaggtcg ccctgcacca gttccacctg gcccgcatca cggtaggcag ccagttccgc
     1681 gcccgcgcgc gcgagcatct cgatcgagag atcgacgccg gtgacgtgct tcgcgtcgcg
     1741 ccgcaaagcg tggagcatgt aacgtccgct gccgcagccc gcgtcgagca cggcctggcc
     1801 ctgcaggttc gccggcatca gcgcctgcat cgcgcgttcc tcggcgcgca tcaccgggtt
     1861 gtgcgcgtgc ggcggatagc tggccgccca cagtgcgtag gcgtcccggg gctcgagcat
     1921 ttcgatcctc gacatcaggc gtgcccttcc gcaagtgcgg gctcgtacgt gcgcgccgcg
     1981 cggcgccacc ggttggagcc cgggctccat ctcgaccagt gcaggatcgg caagcacctt
     2041 cgcgagcagc ttcggctcgc cgtccagcgt cacggggacg gcttccacgc cggctgcttc
     2101 gaaccagccc cagaaatccg gatcggcaat gcgcggcgcg ccatcgcgca ccaccgcgcg
     2161 gatccggttg cgcccgatgc cgacgacgtt gccatgagcg gcgccgccgc catcggcgac
     2221 gaccagcagg tcggcccgat cgccggccgc gaggctgccg tgctgcggca gccgcaggat
     2281 gcgtgcggcg tcggtggtca ccagcgccag cgcatcgcgt gcatcgagtg ctgtcatgtc
     2341 gacggcactc cgcagttcct cgagcaggtc gcgcgcaccg ctgactcgcg aatcggttcc
     2401 cagcagcagc cgtccggcgg cctgcaggcg ccgcgggtcc agcgtttgcc ccaggagatt
     2461 gcgattgctg gtcgggcacc agaccaccgc ggcgccgctt tcgatcacgc ggtcgatgtc
     2521 acgcgcgcgc aatccgacac cgtgcaccag cacgctgttc ggcgcgaggc agccaagcgc
     2581 gtccagttgc gcgagttccg cctgtgcggt tcgatcggtg ccttcggcca gatggatcat
     2641 ccacggccga tcggacggcg tggcgtcgaa actctgccac accggcggtc cgtaaccagg
     2701 ccagccgagg gcgtagctcc agccgtaatc acgcaacagc gccaccggaa aatccggtgc
     2761 atcgagcgcc gggtgccacg ggtcgtggtg caccacgcag gtcgttccgg ccagcaggtt
     2821 cttcaacgcg ccgtgccgca tccgcaaggt cttcggaacg gccagcgcgg cgaccacggc
     2881 ggggtcgcgg aaatgcgact ggaacgccgc gatccatgcg
//
LOCUS       DASSNH010000082         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1445090, whole genome shotgun sequence.
ACCESSION   DASSNH010000082 DASSNH010000000
VERSION     DASSNH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445090"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..559
                     /locus_tag="VFK00_02950"
     CDS             149..559
                     /locus_tag="VFK00_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7591943.1"
                     /translation="MKTLSHGSDLLGRLFLVLLFVISGFGKIGGYAGTAAYMAANGVP
                     GLLLPLVIVLEIGGGILVVLGWHTRIASLLLAGFTILALLLFHMPVTAENQIVFLAEL
                     GVAGGFLVLAAHGAGAWSLDAWLVRRTPAKAVAA"
     gene            complement(787..2280)
                     /locus_tag="VFK00_02955"
     CDS             complement(787..2280)
                     /locus_tag="VFK00_02955"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET7591944.1"
                     /translation="MTNNSSKGGSTTGAGAPAPSDRNSLTIGPNGPIVLHDVHFLEQM
                     AHFNREKVPERQPHAKGAGAFGVFETTEDVSGYTKAALFQSGAQTDMLARFSTVAGEQ
                     GSPDTWRDVRGFSLKFYTDEGNYDLVGNNTPIFFVRDPMKFPHFIRSQKRLPDSGLRD
                     NHMQWDFWTSNPETAHQVTYLMGVRGLPRTWRHMNGYGSHTFMWTNKAGEKFWVKYHF
                     HTEQGMEFFSNAEAAEVAGRDADFHRRDLFNAIARGEHPSWILSVQVMPYAKAKDYRF
                     NPFDLTKVWPHKDYPLIRVGRMTLNRNPENFFAQIEQAAFSPGNTVPGIGLSPDKMLL
                     GRAFAYNDAQRNRIGTNFHQLPVNQPKVPVDTYLFDGQMAYHHSGNAPVHAPNSGGRP
                     WSDETGAVEDGWEADGEMVRSAYTLHAEDDDFGQPGTLVREVFGDAERNALVEQVAGS
                     LLGGVRSPVLERAFEYWKNIDADVGSRIERKVREGSATGPAEGMGEA"
     gene            2522..>3583
                     /locus_tag="VFK00_02960"
     CDS             2522..>3583
                     /locus_tag="VFK00_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7591945.1"
                     /translation="MPYGWISTAILFLPFAAALSTALIGRGNRRRTTWMMTGTAAICL
                     ALSALLFPAIGDGDVLRTVFAWAPALGLDFTLRIDGLAWLFLILVCGIGLLVGIYATY
                     YMPREYPLVRFFSLLLGFMGAMLGIVLSGNVIQLVFFWELTSLFSFLLIGYWHHGKSA
                     RDAARTALIVTVAGGLCLLAGVLVLAHVAGSYDIDAILAAGDHVRESPLYLPALVLIL
                     LGAFTKSAQVPFHFWLPQAMSAPTPVSAYLHSATMVKAGVFLLLRFWPVLAGTEPWFW
                     IVGGVGLATMLVGAWVATFQHDMKAVLAYSTISHLGLITMLAGLGSTLGVVAAIFHTV
                     NHATFKASLFMAAGAVDHET"
BASE COUNT          500 a         1158 c         1187 g          738 t
ORIGIN      
        1 gcgggcgatg ccgcgtcctg gaccgtcggt cccgatgccc tcgacatccg ggccgccgcg
       61 cccagcgaag tgctgttgtt cgacctcgcg ccggcgcaat gaaaccggcg gccacctact
      121 tcaatccgtc ccacaaccgg aggttcacat gaaaaccctc tcccatggtt ccgacctgct
      181 cggccggctg ttcctggttc tgctgttcgt gatttcgggc ttcggcaaga tcggcggcta
      241 tgccggcacg gcggcctaca tggccgcaaa cggcgtgccg ggcctgctgt tgccgctcgt
      301 gatcgtgctc gaaatcggcg gcggcatcct tgtcgtgctc ggctggcaca cccggatcgc
      361 gtcgctgctg ctggccgggt tcacgatcct cgccctgctg ctgttccaca tgccggtcac
      421 cgccgaaaac cagatcgtgt tcctggccga gctgggcgtg gcgggtggct tcctggttct
      481 ggcagcgcat ggcgccggcg cctggagcct ggatgcgtgg cttgtccgcc gcacgccggc
      541 gaaagcggta gccgcctgag ccatgtgacg gctccacgca cgttgtaacg actgcgggtc
      601 cgcggtgatg gctgcggacc cgcaacttcc aggattcgag ttttcaggcg ccggttgggg
      661 aaaccttttt ttgatcgtca ttccgacgaa agccggaacc catgttgatc ttcttccaat
      721 gaaaccaaga tcaaaatgga ccccggcttg cgccggggtg acgagtcggg attgatcagc
      781 gcttcctcag gcctcgccca tcccctccgc tggtcccgtt gcgctgcctt ccctgacctt
      841 ccgttcgatc cgactgccga cgtcggcgtc gatgttcttc cagtactcga acgcacgttc
      901 cagcaccggg ctgcgcacgc cgccgagcag gctgccggcg acctgctcga ccagcgcgtt
      961 gcgctcggca tcaccgaaca cttcgcgcac caaggtaccg ggctggccga aatcgtcgtc
     1021 ctcggcgtgc agcgtgtagg cgctgcggac catttcgcca tcggcttccc agccgtcctc
     1081 caccgcgccg gtttcgtcgg accacggccg tccgccgctg ttcggcgcgt gcaccggcgc
     1141 attgccgctg tggtggtatg ccatctggcc atcgaacagg tacgtatcca ccggcacctt
     1201 cggctggttg accggcaact ggtggaagtt ggtgccgatc cggttgcgct gcgcgtcgtt
     1261 gtaggcgaag gcgcggccga gcagcatctt gtccggcgac agtccgatgc ccggtacggt
     1321 gttgccgggc gagaacgcgg cctgctcgat ctgcgcgaag aagttctcgg gattgcggtt
     1381 cagcgtcatc cggccgacgc ggatcagcgg gtagtccttg tgcggccaga ccttggtcag
     1441 gtcgaacgga ttgaagcggt aatccttggc cttcgcgtac ggcatcacct gcaccgacag
     1501 gatccagctc gggtgctcgc cacgcgcgat ggcattgaac aagtcgcggc ggtggaagtc
     1561 ggcatccctg ccggccacct cggcggcctc ggcgttgctg aagaattcca tgccctgttc
     1621 ggtgtggaag tggtacttca cccagaactt ctcgcccgcc ttgttggtcc acatgaaggt
     1681 gtgggagccg tagccattca tgtgccgcca ggtgcgcggc aggccacgca cgcccatcag
     1741 gtaggtgacc tggtgggcgg tttccgggtt gctggtccag aagtcccact gcatgtggtt
     1801 gtcgcgcagg cccgaatcgg gcaaccgttt ctggctacgg atgaaatgcg gaaatttcat
     1861 cgggtcacgc acgaagaaga tgggcgtgtt gttgccgacc aggtcgtaat tgccttcgtc
     1921 ggtgtagaac ttcagcgaaa agcctcgcac gtcgcgccag gtgtcggggc tgccctgctc
     1981 gccggcgacg gtggagaagc gcgccagcat gtcggtctgc gcgccggact ggaacagcgc
     2041 cgccttcgtg tacccggata cgtcttcggt ggtctcgaac acgccgaagg cgccggcgcc
     2101 ctttgcgtgc ggctggcgtt cgggcacctt ctcgcggttg aagtgcgcca tctgttcgag
     2161 gaagtgcacg tcgtgcagca cgatcgggcc gttcgggcca atggtcagtg aattgcggtc
     2221 gctgggcgcg ggcgcgccgg cgccggtggt cgagccaccc ttgctgctgt tgttcgtcat
     2281 gacttgctcc gttcgagcag atgccaagac atggtgcagc cgccacggtt aaggtttcat
     2341 gcaagccatc gggggacgga ttcgggacgc ggtatcgacc caatggccgc cggcatgtcc
     2401 gtgtggatcc cgcggcgtcg gccactgtcc ggccgctcga cctgcccttg tagactcgaa
     2461 gtcccgatcg cgtcgcgtcg tcgccgtgcg cccgggagca ttggcctgga tcgtccggat
     2521 gatgccttac ggctggatct caaccgccat cctgtttttg ccgttcgcgg ccgcgctgtc
     2581 cacggcgctg atcggccgcg gcaaccgccg gcggacgacg tggatgatga ccggcaccgc
     2641 cgccatttgc ctggcccttt cggcgctgct gtttcccgcg attggcgacg gcgacgtgct
     2701 tcgcacggtt ttcgcctggg cgccggcgct cggcctcgat ttcacgctgc ggatcgacgg
     2761 actcgcctgg ctgttcctga tcctggtttg cggcatcgga ttgctggtcg gcatctacgc
     2821 gacctattac atgccgcgcg agtacccgct ggtccggttt ttttcgctgc tgctgggttt
     2881 catgggcgcg atgctgggca tcgtgctgtc cggcaacgtg atccagcttg tgttcttctg
     2941 ggaactgacc agcctgttct cgttcctgtt gatcggttac tggcaccacg gcaagtcggc
     3001 gcgcgacgcc gcgcgcacgg cactgatcgt caccgtcgcg ggcggactgt gcctgctggc
     3061 cggcgtgctg gtgctcgcgc acgtcgcggg gagttacgac atcgatgcga tcctcgccgc
     3121 gggcgaccac gtccgcgaga gcccgctgta cctgccggcg ctggtgctga tcctgctagg
     3181 cgcgttcacc aagagcgcgc aggtgccgtt ccacttctgg ctgccgcagg cgatgtcggc
     3241 ccccacgccg gtgtcggcgt atctgcactc ggcaacgatg gtgaaggccg gtgtgttcct
     3301 gctgctgcgg ttctggccgg tgctggcggg cacagagccg tggttctgga tcgtgggggg
     3361 ggtcggtctc gcgacgatgc tggtgggcgc ctgggtcgcg acgttccagc acgacatgaa
     3421 agcggtgctg gcgtattcca cgatcagcca cctcggcctg atcacgatgc tggccgggct
     3481 cggcagcacg ctcggcgtgg tggcggcgat cttccacacc gtcaaccacg cgactttcaa
     3541 ggcttcgttg ttcatggccg cgggcgcggt ggaccacgaa acc
//
LOCUS       DASSNH010000083         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_923183, whole genome shotgun sequence.
ACCESSION   DASSNH010000083 DASSNH010000000
VERSION     DASSNH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923183"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /gene="glmS"
                     /locus_tag="VFK00_02965"
     CDS             complement(<1..1653)
                     /gene="glmS"
                     /locus_tag="VFK00_02965"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7591946.1"
                     /translation="MCGIVAGCTQRDVVPILLAGLHALEYRGYDSAGLAVLEEGRLTR
                     LRTPGKVAALDALWQSSPAHGRTGIAHTRWATHGVPNEANAHPHVSRDTVAVVHNGII
                     ENHVELRAELSAAGYAFSSETDTEVIAHLVHKLVADGKGLHDAVFEATRRLRGAYAIA
                     VMHADVPGHVVGARRGSPLCVGIGIGEHFLGSDVQALIQVTNRIVYLDEGDTVEITAD
                     CLAIRDAAGRPVIRRVRESDLSRDAVQRGEFRHYMLKEIHEQPTAVANTLEGRIANGH
                     VLPNIFGVDAERLLQQVRHVHVVACGTSYHAGMVARYWLEGIAGIPCTVEVASEYRYR
                     HVVVMPDTLFLAISQSGETADTLAALRDGRSRGYVGSFAICNVPESSLVREADLSLMT
                     RAGPEIGVASTKAFTTQLSALALLVLELARIQGIAEGKLAELTAELESLPRKLDEALK
                     LEPVIAKLAEAFVDKHHALFLGRGAHYPVAMEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDEDMPIIAVAPNNQLLEKLKSNLEEVRARGGQLYVFADAG"
     gene            1877..>3252
                     /locus_tag="VFK00_02970"
     CDS             1877..>3252
                     /locus_tag="VFK00_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HET7591947.1"
                     /translation="MFRLFRQRALHWQLLLVLVEFGILTAGVYAAVVLRYWGDADTQS
                     AFNQVLHWRAPLVAGVVILAMASLGLYQIHLRAGWLGRLSRQGIAFVLGGVALTVLYY
                     AYPDAYLGRGVLALALVLGYLAVALWRVLFLGFVDADLFKRRVVMLGAGERAAVVARK
                     MRRRTDQRGFKVLGYVPVGSDPISVPATSLLHPEGPLCRWAVGLGVDEIVVGPDDRRG
                     TLPVEALLECKQRGMAVTELAEFFEREAGTIKMDLTNPSWLVFSDGFNTSPVRRTIKR
                     TFDMAVSLLVLMVAWPLMLLTALAIRLESGRGAPILYRQERIGEYGKRFHVIKYRSMQ
                     TDAECDGVARWAAKDDHRVTRVGRFIRKTRLDELPQLWNVLRGDMSIIGPRPERPQFV
                     DDFNTRIRYYPLRHCVKPGLTGWAQLRYPYGSSLEDAEEKLKFDLFYVKNHNFVFDLA
                     ILVQTVEVV"
BASE COUNT          517 a         1022 c         1102 g          611 t
ORIGIN      
        1 gcccgcatcg gcgaagacgt acaactggcc gccgcgcgcg cggacttcct ccaggttcga
       61 cttcagcttt tcgagcaact ggttgttggg cgccaccgcg atgatcggca tgtcttcgtc
      121 gaccagcgcc agcggaccat gcttcagttc gccggccggg tatgcctcgg cgtggatata
      181 ggagatttcc ttgagcttga gcgcgccttc catcgcgacc gggtagtggg ctccgcgccc
      241 gaggaacagc gcgtggtgct tgtcgacgaa cgcttcggcg agtttggcga tcacgggctc
      301 gagtttgagc gcctcgtcga gcttgcgcgg caagctttcg agttcggcgg tcaactcggc
      361 gagcttgccc tcggcaatcc cctggatacg tgcgagttcc agtaccagca gcgccagggc
      421 gctgagctgg gtcgtgaagg ccttggtcga cgccacgccg atttcggggc cggcgcgggt
      481 catcagggaa aggtcggctt cacgcaccag gctcgactcg ggcacgttgc agatcgcgaa
      541 cgacccgacg taaccacgcg accggccgtc acgcaaggca gccagcgtat cggcagtttc
      601 gccagactgg gaaatggcga ggaacaaggt gtccggcatc accaccacgt ggcggtagcg
      661 gtattcgctg gccacctcga cggtgcaggg aatgccggcg atgccttcca gccagtaccg
      721 ggccaccatc ccggcgtggt agctggtgcc gcaggcgacg acgtggacgt gccgcacctg
      781 ctgcagcagc cgctcggcgt ccacgccgaa gatattggga agcacgtggc cgttggcgat
      841 gcggccttcc agggtgttgg cgacggcggt cggctgctcg tggatttcct tcagcatgta
      901 atgccggaac tcgccgcgct ggacggcatc ccgcgagagg tcgctttcgc gcacgcggcg
      961 gatcaccggt cgacccgctg cgtcccggat cgccaggcaa tcggcggtga tttcgaccgt
     1021 atcgccttcg tcgaggtaca cgatgcggtt ggtcacctgg atcagggcct gcacgtccga
     1081 tccgaggaag tgttcgccga taccgatgcc gacgcacagc gggctgccgc gtcgcgcgcc
     1141 gaccacgtgc cccggcacgt cggcatgcat taccgcgatc gcataggcgc cgcgcagccg
     1201 gcgggtagcc tcgaacacgg cgtcgtgcag gcctttgccg tcggccacca gcttgtgcac
     1261 caggtgcgcg atgacttcgg tgtcggtctc cgacgagaag gcgtagcccg ccgctgacag
     1321 ctcggcgcgg agttccacgt ggttctcgat gatcccgttg tggacgaccg cgacggtatc
     1381 ccgcgaaacg tgcggatgcg cgttggcttc gttcgggacc ccgtgggtgg cccagcgggt
     1441 atgcgcgatg ccggtgcgcc cgtgggcggg ggaggactgc cacagcgcat ccagcgccgc
     1501 gaccttgccc ggggtccgaa ggcgggtcag gcgaccctcc tccaggactg ccaggccggc
     1561 cgagtcgtac ccccggtact cgagggcgtg cagccctgcg agcaggatcg ggacgacgtc
     1621 acgttgggtg cagccggcga cgattccgca catggtgagc ttcgatttcg ttgggccagc
     1681 aaagatacac ggaagcgcac cttgccagca gcccgttcat ttttcccgca aggcatgttt
     1741 cttgcaggaa ctttcaaccg ttttcttatc gaactttgaa ctgcgtcacg gttgtggtat
     1801 aaatcccgtc ccactcgcga accgggctga ccaccgacgc agggacaagg catacaacaa
     1861 ggacgggggc atcgacatgt ttcgattgtt ccgacagaga gcactgcatt ggcagttgtt
     1921 gttggtattg gtcgagttcg gaatcctcac cgccggcgtg tatgcggcgg tggtgctgcg
     1981 ctactggggc gacgctgaca cccagtcggc gttcaatcag gtattgcatt ggcgcgcgcc
     2041 gctggtagcc ggcgtcgtga tcctggcgat ggcgtcgctc gggctctacc agatccacct
     2101 gcgggctggc tggctgggcc ggctcagccg gcagggcatt gcgttcgtgc tcggtggggt
     2161 cgcgctgacc gtcctttact acgcctaccc ggatgcctat ctgggacgtg gcgtgctcgc
     2221 gctcgcgctg gtgctcggat acctggcggt ggcgctgtgg cgcgtgttgt tcctcggctt
     2281 cgtcgatgcc gacctgttca agcgccgcgt ggtgatgctc ggcgccggcg agcgcgccgc
     2341 cgtcgtggcg cgcaagatgc gccgccggac cgaccaacgc ggtttcaagg tgctgggcta
     2401 cgttcccgtc gggtcggacc cgatcagcgt gccggcgact tccttgctgc acccggaagg
     2461 tcccctgtgc aggtgggccg tgggcctggg cgtggacgaa atcgtggtgg gacccgatga
     2521 ccgtcgcggc acgctgcccg tcgaggcatt gctcgagtgc aagcagcgcg ggatggccgt
     2581 gaccgaattg gccgagttct tcgagcgcga ggccggcacc atcaagatgg acctcaccaa
     2641 tccgtcgtgg ctggtgttct ccgacggctt caatacgtcg ccagtgcgcc gcacgatcaa
     2701 gcgcacgttc gacatggccg tctccctgct ggtgttgatg gtggcatggc cgctgatgct
     2761 gctgactgcg ctagcaataa ggctggaatc cggccgcggc gcaccgatcc tctatcggca
     2821 ggagcggata ggcgaatacg gcaagcggtt ccatgtcatc aagtaccgca gcatgcagac
     2881 cgacgcggaa tgcgacggcg ttgcgcgctg ggcggccaag gacgaccacc gcgtcacccg
     2941 ggtgggtcga ttcatccgga agacccgcct cgacgagttg ccgcagttgt ggaacgtgct
     3001 gcggggcgac atgagcatca tcggcccgcg ccccgagcgc ccgcagttcg ttgacgattt
     3061 caacacacgc attcggtact atccgttgcg gcactgcgtg aaacccggcc tcaccggctg
     3121 ggcccagctt cgctatccct atggctcgtc gctcgaggat gccgaggaga agctgaagtt
     3181 cgacctgttc tacgtcaaga accacaactt cgtgttcgat ctggccatcc tggtgcagac
     3241 ggtggaggtg gt
//
LOCUS       DASSNH010000084         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_437958, whole genome shotgun sequence.
ACCESSION   DASSNH010000084 DASSNH010000000
VERSION     DASSNH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437958"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFK00_02975"
     CDS             <1..482
                     /locus_tag="VFK00_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591948.1"
                     /translation="KAMQRKPGSLRNKLMVASALVATAVLATPAAMARGYHHNDDDTA
                     KVVGALVVGAVIGGVLASSSHHDNYYRGGYAYPARTYYPSNYYGSYPAYSYNSYPAYS
                     YNTYPAYPYGYYAPAPRYRSGISIGVVYSNHGDRSHYYRGHSSRDYRGGHGHYYHGH"
     gene            complement(628..1686)
                     /locus_tag="VFK00_02980"
     CDS             complement(628..1686)
                     /locus_tag="VFK00_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7591949.1"
                     /translation="MQHDIAPQPLEEASALPARFYVGAESTERDRRHVFAHGWQLLAR
                     AGQLRESGDHVVGDIAGVPLLLVRGDDGTLRALHNVCRHRAGPLALCDGRAAKRLRCH
                     YHGWSYALDGRLLSATEMDGARDFDVASIRLPEARVAEWRGLVFVALDPRTSFAETVA
                     EVDARLGDRPIADYAFHRHYEYDIACDWKNYIDNYLEGYHVPHIHPELNRLLDYRDYV
                     VETGRWHSLQHSPLASDAALYGDGEALYWHIWPNTLLNVLPDRMQTNRVMPLAPGRCR
                     VTFDYFYPAGTADLESRHASDHAFSDLVQRQDVDMCEIVQRGLASGSYHAGRLNPRRE
                     NGVHHFHELLREAYRAVE"
     gene            1839..3116
                     /gene="egtB"
                     /locus_tag="VFK00_02985"
     CDS             1839..3116
                     /gene="egtB"
                     /locus_tag="VFK00_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET7591950.1"
                     /translation="MPALCLPGADRLGSDLVSPYAHVRGRTTALCAPLAPEDTVPQSM
                     PDASPAKWHLAHTTWFFEEFVLGHFSRGYRRFREGWDYLFNSYYQTVGPMHARPQRGL
                     LTRPTLDEVLDYRAHVDEAMRQLLMERGDDPDLHARVTLGLNHEQQHQELLLTDIQHL
                     FSMNPLQPVYRESPAVPASPPLDLRFIAGRHGIVETGQAGEGFAYDNEGPRHRELLQP
                     HAIANRCVTNSEFHAFIDDGGYGNPVLWLSEGWDTVRREGWKHPLYWDDDLETAFTLA
                     GRRAIDPAAPVCHVGFFEADAFARWAGARLPTEAEWETLARDLQVEGNFADAGVLHPL
                     PAGPADGARGGPLQMFGDVWEWTASPYVGYPGYRPAAGAIGEYNGKFMCGQWVLRGGS
                     CATPAGHVRASYRNFFFPAARWQFSGLRLAKDA"
     gene            3113..4108
                     /gene="egtD"
                     /locus_tag="VFK00_02990"
     CDS             3113..4108
                     /gene="egtD"
                     /locus_tag="VFK00_02990"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET7591951.1"
                     /translation="MNLPGPVARKIHLDDRRPRRDDILADAVRGLAADPKRLPSKYFY
                     DAQGSKLFERICEQPEYYPTRVELEIMREHVGDIAAALGPEVRLVEYGSGSGLKTRLL
                     LRHLDSPVAYVPVEISRTAVSNSVAALGSEFPGIEMLPVCADFTRPITLPRAARAPCQ
                     TVVYFPGSTLGNFEPEQALRLLRQMRSEAGQGGGKLPGGVLIGIDLVKDKASLEAAYN
                     DAAGVTAAFTLNLLARLNREIGADFDLDGFRHRARWHPLAERIETHIVSTRAQDVHID
                     GRTFHFGEGEAMLVELSCKYTLESFARLAARAGLRVAKVWTDPARQFSVQWLVAE"
     gene            4209..>4822
                     /locus_tag="VFK00_02995"
     CDS             4209..>4822
                     /locus_tag="VFK00_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET7591952.1"
                     /translation="MRCIRWGLAWLALAMCGTAFAAAGNPYARDPDQPVDQAYTAKIA
                     KYTTQPDFNTPLTDYLPASQTVPTPEAVLGDVAGAPNMLPYAEDVYRYFRMLAKATPR
                     VQVFTIGKTEEGREMIAVAIADESLMKDLDGDKARLAQLADPREPGMDDAKAAALIKQ
                     TAPVYYITGTIHSTETGAPTALMELAYRLAVDDAPYIQYIRSHM"
BASE COUNT          802 a         1616 c         1606 g          798 t
ORIGIN      
        1 cgaaagccat gcaacgtaaa cccggttcgc ttcgcaacaa gctgatggtc gcctccgcgc
       61 tggtcgcgac tgcggtattg gccacgccgg ccgccatggc acgcggctac caccacaacg
      121 acgatgacac cgcgaaagtg gtcggcgcgc tggtggtggg cgccgtgatc ggcggcgtgc
      181 tggcgagctc cagccaccac gacaactatt accggggcgg gtacgcctat ccggcgcgga
      241 cctactatcc gtcgaactac tacggaagct atcccgcgta ctcgtacaac agctacccgg
      301 cgtattccta caacacctat ccggcgtatc cgtacggcta ctatgcgccg gcaccacggt
      361 accgcagcgg catcagcatc ggcgtggtgt actccaacca cggcgaccgt tcgcactatt
      421 atcgcgggca ctcctcgcgc gattaccgcg gtggccacgg ccactactat cacgggcact
      481 gacaccggat cgtcccgcgt aggctccccg gcccggttgc cctgcaaccg ggctttttca
      541 tatccgcgtc ggctcccgtt gtagaagccg ccatgcgcgc aataagggca tccggtgcgg
      601 gaccgccgaa acaggcgctg cgcccgacta ttcgacggct ctatacgcct cgcgcaacag
      661 ctcgtggaaa tggtgcacgc cgttttcgcg cctcggattc aggcggccgg cgtggtacga
      721 gcccgacgcg aggccgcgct gcacgatttc gcacatgtcg acgtcctggc gctggacgag
      781 gtcgctgaag gcgtggtcgc tggcgtggcg cgattccagg tcggcggtgc ccgccggata
      841 gaaataatcg aacgtgacgc ggcaacgtcc cggcgccagc ggcatcaccc ggttggtctg
      901 catccggtcc ggcagcacgt tgaggagcgt gttgggccag atgtgccaat acagcgcttc
      961 gccgtcgccg tacaacgcgg cgtcgctcgc gagcgggctg tgctgcagcg aatgccagcg
     1021 ccccgtttcg accacgtagt cgcggtagtc cagcagccgg ttcaattccg ggtggatgtg
     1081 cggcacgtgg tagccctcga ggtagttgtc gatgtagttc ttccagtcgc aggcgatgtc
     1141 gtactcatag tggcgatgga aggcatagtc cgcgatgggc cgatcgccca gccgcgcgtc
     1201 gacctccgcg acggtttccg cgaacgacgt ccgtggatcc agcgcaacga acaccagccc
     1261 gcgccactcg gccacgcggg cttcgggaag gcggatggag gcgacgtcga agtcgcgcgc
     1321 tccgtccatt tcggtcgcgc tcaacaggcg cccgtcgagt gcataactcc agccgtggta
     1381 gtggcagcgc agccgtttcg cggcgcggcc gtcgcagagc gcgagcggtc cggcacgatg
     1441 cctgcacacg ttgtgcagcg cgcgcagcgt gccgtcgtcg ccgcgcacga gcagcagcgg
     1501 tacgcccgcg atgtcgccga cgacgtggtc gccggattcc ctgagttggc ccgcgcgtgc
     1561 gagcaattgc cagccatgcg cgaaaacgtg gcggcggtcg cgctcggtac tctccgcgcc
     1621 gacgtaaaaa cgcgcgggca gcgcggaggc ttcctcgagc ggctgtggcg cgatgtcatg
     1681 ctgcatggtc gggttccgca gggaagaagg gcgatgccgc atggcggcaa cgtgctgggc
     1741 gggctcgatg gccgcatcgg ctgaacatgt ggagtggcag cgttaacatg gtccgtagta
     1801 gggtaccaac ggatcgcgcc tcgcgaggaa acatccgcat gcccgcactt tgcctcccgg
     1861 gggccgatcg cctcggatcc gacctggttt cgccctacgc gcacgttcgc ggccggacca
     1921 ccgccctgtg cgccccgctg gcgcccgagg acacggtccc gcagtccatg cccgacgcca
     1981 gccccgcgaa gtggcacctc gcgcatacga cgtggttctt cgaggagttc gtgctcggcc
     2041 acttctcgcg gggttaccgc cgcttccgcg aaggctggga ctatctcttc aattcctatt
     2101 accagacggt cggcccgatg cacgcgcgcc cgcagcgggg actcctgacc cgcccgacgc
     2161 tggacgaagt gctcgactac cgcgcgcatg tcgacgaggc gatgcggcag ttgctgatgg
     2221 agcgcggcga cgatccggac ctgcacgcgc gggtcacgct gggcctcaat cacgagcagc
     2281 agcaccagga attgctgctc accgacatcc agcacctgtt ttcgatgaat ccgttgcagc
     2341 cggtgtaccg ggaatcgccg gcagtgccag cgtccccgcc gctggatttg cgcttcatcg
     2401 ccggccgcca cggcatcgtc gagaccgggc aagccggcga gggcttcgcc tacgacaacg
     2461 aaggcccgcg gcatcgtgag ttgctgcagc cgcatgcgat cgcgaaccgt tgcgtgacga
     2521 actcggaatt ccatgcgttc atcgatgacg gaggctacgg caatccggtg ctgtggctct
     2581 cggagggttg ggacacggtg cggcgcgaag gctggaagca tccgctgtac tgggacgacg
     2641 acctggaaac cgcgttcacc ctggcggggc ggcgcgcgat cgaccccgcc gcacccgtgt
     2701 gccatgtcgg tttcttcgaa gccgatgcct ttgcccgctg ggccggtgcg cggttgccga
     2761 cggaagccga atgggagacc ctcgcccggg atcttcaggt cgagggcaat ttcgccgatg
     2821 ccggcgtgct gcatccgttg ccggcgggac ccgccgatgg cgcgcgcggc ggtccgttgc
     2881 agatgttcgg cgacgtgtgg gaatggacgg ccagcccgta cgtcggatat ccgggctacc
     2941 ggccagctgc cggcgcgatt ggtgaataca atggcaagtt catgtgcgga cagtgggtat
     3001 tgcgcggcgg ttcgtgcgcg accccggctg gccacgtccg cgcgagttac cgcaactttt
     3061 tctttcccgc ggcacgctgg cagttcagcg gcctgcggct ggcgaaggac gcatgaatct
     3121 cccaggcccg gttgcccgaa agatccacct cgacgatcgg cgtccccgcc gggacgatat
     3181 tcttgccgat gccgtgcgcg gcctggccgc ggatccgaag cggttgccgt cgaagtattt
     3241 ctacgacgcg cagggctcga agctgttcga gcgcatctgc gaacaaccgg agtactaccc
     3301 gacccgcgtc gagctcgaga tcatgcgcga gcacgtcggc gacatcgccg ccgcgctcgg
     3361 gcccgaggtc cggctggtcg aatatggcag cggcagcggg ctcaagaccc ggctgctgct
     3421 gcggcacctc gattcgccgg tggcctacgt gccggtcgag atttcgcgca cggcggtgtc
     3481 caacagcgtg gccgcgctgg gcagcgaatt cccgggcatc gagatgctgc cggtctgcgc
     3541 ggacttcacg cggccgatca ccttgccgcg ggcggcgcgt gcgccctgcc agacggtcgt
     3601 ctatttcccg ggttcgacgc tcggcaattt cgaacccgaa caggcgttgc ggctgttgcg
     3661 gcagatgcgc agtgaagccg ggcagggcgg cggcaagctg cccggcggcg tcctcatcgg
     3721 catcgacctc gtcaaggaca aggcctcgct cgaggccgcc tacaacgatg ccgccggcgt
     3781 caccgcggcg ttcaccctca acctgctggc gcgcctcaat cgcgaaatcg gcgccgactt
     3841 cgacctcgat ggtttccgcc atagggcgcg ctggcatccg ctggccgaac gcatcgagac
     3901 ccatatcgtc agcacccgtg cccaggacgt ccacatcgat ggccgcacgt tccacttcgg
     3961 ggaaggcgag gcgatgctgg tcgaactgag ttgcaagtac accctggaga gtttcgcccg
     4021 gctggcggca cgcgccggac tgcgggtggc gaaggtgtgg accgatccgg cccggcagtt
     4081 cagcgtgcag tggctggtcg cggaatgaga cgaacgcgcg gggcgggtcc ctccatgcct
     4141 ttcgacaccc tcgccacggc ctgaatttgg ctacgcttgc cgcaatcgga acaagcggga
     4201 gcggggatat gcgttgcatt cgatggggat tggcgtggct ggcgttggcc atgtgcggca
     4261 cggcgttcgc cgccgccggc aatccctacg cgcgcgaccc cgaccagccg gtcgaccagg
     4321 cgtataccgc aaagatcgcg aaatacacca cgcagccgga cttcaacacc ccgctgaccg
     4381 attacctgcc ggcgtcgcag accgtaccga cgcccgaggc ggtgctggga gacgtcgccg
     4441 gcgcgcccaa catgctgccg tacgcggaag acgtgtaccg ctatttccgg atgctggcca
     4501 aggccacgcc acgggtgcag gtcttcacga tcggcaagac cgaggaaggc cgcgagatga
     4561 tcgcggtggc aatcgccgac gaatcgctga tgaaggacct ggacggcgac aaggcccgcc
     4621 tcgcgcagtt ggccgatccg cgcgagccgg gaatggacga cgccaaggcc gcggcgctga
     4681 tcaagcagac cgctccggtc tattacatca ccgggacgat ccattcaacc gagaccggcg
     4741 cgcccaccgc gctgatggag ctcgcgtacc gcctcgccgt cgacgacgcg ccgtacatcc
     4801 agtacatccg ctcgcacatg at
//
LOCUS       DASSNH010000085         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_800974, whole genome shotgun sequence.
ACCESSION   DASSNH010000085 DASSNH010000000
VERSION     DASSNH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800974"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1217)
                     /locus_tag="VFK00_03000"
     CDS             complement(231..1217)
                     /locus_tag="VFK00_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7591953.1"
                     /translation="MAKDPAPNVLLARVGRRLKILGIAIIAVAVAALAVYLIAPRVVL
                     HGAIAWQTWRDRLHRETVQVGETRWVYLAGGKKDGPPLVLLHGYGGSYANWLLAARYL
                     TGNFRLIIPDLPGWGESTRLPDADYGYAAQVDRLHGFVDALDLGQIALAGHSMGGAIA
                     GLYAAKYPKDVAALVLIDSSGVRFKKNAFVRELEAGKSPFDIDDRAGFRHLETMLFDD
                     PPSVPPRIQDVFIERSQQNRAFRDKVLREITAPDARFALEPKLADITAPTLAIWCMQD
                     AIIDPSALDAIRKGLTHSPDIGVTQLHGCGHMSIMEKSRDVARAITHFVLLP"
     gene            complement(1224..3107)
                     /locus_tag="VFK00_03005"
     CDS             complement(1224..3107)
                     /locus_tag="VFK00_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7591954.1"
                     /translation="MRLLSTLALGAFVLPVAAQAADPHSYAQPRDVLVKHLELNLDVD
                     FAHEQLDGVATLTLDWKNPGAQELVLDTSALQIASVEALDAQGHAHTLPFKLAEPVKA
                     MGSKLTIEAPKHPHEVRIAYKTSPDASGLQWLDPAQTAGKHHPFLFSQSESVHARSWI
                     PLQDSPAVRFTWHAQVHAPKDLRVVMSAPNDPKHPLDGSFEFTQAHPVPSYLMAIAAG
                     DLDVRETGPRSAVYAEPSVVDKAAHEFEDTEKMIETAESLYGPYRWGRYDILVLPPSF
                     PYGGMENPNMTFATPTVLVGDKSLVSLVAHELAHSWSGNLVTAASWRDIWLNEGITTY
                     VQGRITEALYGERQATEETLLAIRNLEKTIGKMPGNAQRLAPEPRKIEADDELSDVAY
                     DKGSWFMRTLEQKFGRQDFDRYLEGYFNHFAWHSITTEQMLAYLKANLIGKYPGKMDW
                     NEVEAWVYGTGIPKDAPIPDSPRFDAIDSERTEFLDGKLAADKLDAKDWNTQEWMYFL
                     DRLPDQPPLDKMQALDAAWHLTGTPNAEIGMRWYVHAIRAGDKAVWPAAAEHMTRIGR
                     LYLAMPLYKAFVKTPDGFAYAEQVYAKARDGYHPLTRMAVERLFEKTRAGEDKDHAGA
                     GGT"
     gene            3240..3923
                     /locus_tag="VFK00_03010"
     CDS             3240..3923
                     /locus_tag="VFK00_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7591955.1"
                     /translation="MYTLYVANKNYSSWSLRPWVLLRERGIGFEEELVQFEAGSSWKS
                     FRKFSPSGKVPCLVDGATVVWDSLAIAEYVAEGRPGVWPADKEARAFARSAAAEMHSG
                     FAALREQCTMNCGIRVQLRRIDESLSRDLARVNELWNEGLRRFGGPFLAGAEFTAADA
                     FYAPVAFRVQTYSPPLDATANAYAQRLRALPSMRDWYASALAETWRDAAHEAEVANWG
                     TITEDLRAA"
     gene            complement(3936..4577)
                     /locus_tag="VFK00_03015"
     CDS             complement(3936..4577)
                     /locus_tag="VFK00_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7591956.1"
                     /translation="MLTLYDYPASQNAWKVRLLLHHLDRPYRTEIVSIFEGKGRSDDY
                     LAISPTGTVPAIRLEDGRALAESNAILAYLADGTPYLPSGPFERAKVQQWLHFEQERV
                     ESVIGSLRYWTLTGKLAQRPPALVEMKRTGALHTLGILERELAARPFLANDRYSIADM
                     SVFAYASRAEEAGITLDPYPHFRAWIARVEAQPGFLAAMHPYSDDPHSAGELP"
     gene            complement(4663..>5222)
                     /locus_tag="VFK00_03020"
     CDS             complement(4663..>5222)
                     /locus_tag="VFK00_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET7591957.1"
                     /translation="VLRGIFLGRPEEIRWFYEDGGASRILPDKWQRYVSAIPPAERGQ
                     MLEAYWKRLTSGDEAVRVAAARAWGSWEGSALTLAESPETEAEFSATGVAVSLARIEA
                     HYFRNQVFLEPGQLLRDIDRIPHIPGVIVHGRYDVICPAKNAFDLHQAWPEAEFHIVL
                     SGHAASEPAIVDKLVEATDRFAGRF"
BASE COUNT          812 a         1735 c         1780 g          895 t
ORIGIN      
        1 tcccggcccc ggcaccacgc cggtgacgtt gatggcctgt agggtgggga tcgaggacgg
       61 ggaagcgggc gtcgcctggc tggacgcctg cgctgcggcc ggtggttgcg ccgccgcggc
      121 agcgcaggcg aagaacaaca gcaggatggc gggaacacgg ggcatgcagc aaagactttt
      181 ccgccaccgg ggagttccgt ggcgcgacga gggcgtgcct cgcaccgggc tcacggcaac
      241 agcacgaaat gcgtgatcgc gcgcgccacg tcgcgcgact tttccatgat cgacatgtgg
      301 ccgcaaccat gcaattgggt gacgccgatg tcgggcgaat gggtgagtcc cttgcggatc
      361 gcatccagcg ccgacggatc gatgatggcg tcctgcatgc accagatcgc gagcgtgggc
      421 gcggtgatgt cggcgagctt cggctcgagc gcgaagcgcg catcgggcgc ggtgatctcg
      481 cgcagcacct tgtcgcggaa tgcacggttc tgctgcgagc gctcgatgaa aacgtcctgg
      541 atccgcggcg gcaccgaggg cggatcgtcg aacagcatcg tttcgaggtg ccggaatccg
      601 gcacgatcgt cgatgtcgaa cgggctcttg ccggcttcca gctcgcgcac gaacgcgttc
      661 ttcttgaacc ggacgccgga gctgtcgatc agcaccagcg ccgcgacatc cttcgggtat
      721 tttgcggcgt acagtcccgc gatcgcgccg cccatcgaat gcccggccag ggctatctgc
      781 cccaggtcca gcgcatcgac gaacccgtgc aggcgatcca cctgcgcggc atagccgtag
      841 tcggcatccg gcaggcgggt ggattcgccc cagcccggca ggtcggggat gatcaaccgg
      901 aaattgccgg tgaggtagcg cgccgccagc aaccagttgg cgtaggaacc gccgtagccg
      961 tgcagcaaca ccagcggcgg gccgtccttc ttgccgcccg cgaggtatac ccagcgggtc
     1021 tcgccgacct gcaccgtctc cctgtgcagt cggtcgcgcc acgtctgcca cgcgatcgcg
     1081 ccatgcagca ccacgcgcgg cgcgatcagg tacacggcaa gcgccgccac cgccaccgcg
     1141 atgatcgcga tgccgaggat cttgaggcgc cgcccgacgc gcgccagcag cacgttgggt
     1201 gcgggatcct tcgccatgcc gcgtcaggtt ccgcccgcgc cggcgtggtc cttgtcctcg
     1261 cccgcgcgcg tcttctcgaa cagccgctcg accgccatcc gcgtgagcgg gtggtagcca
     1321 tccctggcct tggcatagac ctgctcggca tacgcgaaac cgtcgggcgt tttgacgaat
     1381 gccttgtaca agggcatggc caggtagagc cgtccgatcc gcgtcatgtg ctcggccgcc
     1441 gcgggccaca ccgccttgtc gccggccctg atcgcatgca cgtaccagcg catcccgatc
     1501 tcggcgttgg gcgtccccgt caggtgccac gcggcgtcga gggcctgcat cttgtcgagc
     1561 ggcggctggt cgggcaggcg gtcgaggaag tacatccatt cctgggtgtt ccagtccttc
     1621 gcgtccagct tgtcggcggc cagcttgcca tcgaggaatt ccgtacgctc gctgtcgatc
     1681 gcatcgaacc ggggcgagtc ggggatcggc gcgtccttcg ggatgccggt gccgtacacc
     1741 cacgcctcga cctcgttcca gtccatcttg ccggggtatt tcccgatcag gttcgccttg
     1801 aggtacgcca gcatctgttc ggtcgtgatg ctgtgccagg cgaagtggtt gaaatagcct
     1861 tcgaggtacc ggtcgaaatc ctgccgcccg aacttctgct cgagcgtccg catgaaccag
     1921 gagcccttgt cgtaagcgac gtcggagagc tcgtcgtcgg cttcgatctt gcgcggctcg
     1981 ggcgcgaggc gctgcgcgtt cccgggcatc ttgccgatgg tcttctcgag gttgcggatc
     2041 gccagcaggg tttcttcggt ggcctggcgc tcgccgtaca gggcttccgt gatccggccc
     2101 tgcacgtagg tggtgatgcc ctcgttcagc cagatgtcgc gccacgacgc cgcggtcacc
     2161 aggttgccgg accacgaatg cgcgagttcg tgcgccacca gcgacaccag cgacttgtcg
     2221 cccaccagca cggtcggcgt cgcgaacgtc atgttcgggt tttccatgcc gccgtagggg
     2281 aacgagggcg gcagcaccag gatgtcgtag cggccccagc ggtacggacc gtacagcgac
     2341 tcggcggttt cgatcatctt ctcggtgtct tcgaactcgt gcgcggcctt gtcgacgacc
     2401 gacggttcgg cgtacaccgc gctgcgcggg ccggtctcgc gcacgtccag gtcaccggcg
     2461 gcgatcgcca tcaggtacga aggcacgggg tgggcctggg tgaattcgaa ggaaccatcg
     2521 agcgggtgct tcggatcgtt gggcgcgctc atcaccaccc gcaggtcctt cggcgcgtgg
     2581 acctgcgcgt gccacgtgaa gcggacggcc ggagagtcct gcagcggaat ccacgagcgc
     2641 gcgtgcaccg attcggattg ggagaacagg aacgggtggt gcttgccggc ggtctgcgcc
     2701 gggtcgagcc attgcaggcc cgaggcatcg ggcgaggtct tgtacgcgat ccggacttcg
     2761 tgcgggtgct tcggcgcctc gatcgtgagc ttcgacccca tcgccttgac cggctcggcc
     2821 agcttgaacg gcagggtgtg cgcatggccc tgcgcatcca gcgcttccac ggaggcgatc
     2881 tgcagcgcac tggtgtccag caccagttcc tgcgccccgg gattcttcca gtccagcgtg
     2941 agggtcgcga cgccgtcgag ctgctcgtgg gcgaagtcga cgtcgaggtt caattccagg
     3001 tgcttgacca gcacgtcgcg cggctgcgcg tacgagtgcg gatccgcggc ctgcgcggcg
     3061 accggcagca cgaacgcgcc cagggcgagg gtggaaagaa ggcgcatcgc ggcgctcctg
     3121 tcgatcagcg aaacggtcag tatgccatcc gaccgcggcg cctgcgcatg ccgccaccgg
     3181 gtgcggcgag ttccccggtg cggcgtagaa tccggcgctt cgccaccggg agcggtccca
     3241 tgtacaccct ctacgtcgcc aacaagaact attcctcgtg gtcgctgcgc ccgtgggtgc
     3301 tgctgcgcga gcgcggcatc gggttcgagg aggagttggt gcagttcgaa gccggttcga
     3361 gctggaagtc gttccgcaag ttttcgccca gcggcaaggt gccctgcctc gtcgatggcg
     3421 ccacggtggt gtgggactcg ctggcgatcg ccgaatacgt cgccgaaggc cgccccggcg
     3481 tctggccggc cgacaaggag gcgcgggctt tcgcgcgctc ggccgccgcg gagatgcatt
     3541 cgggtttcgc agcgcttcgc gagcagtgca ccatgaattg cggcatccgg gtgcaactgc
     3601 gccggatcga cgagtccctg tcgcgcgacc tcgcacgcgt caacgagctg tggaacgagg
     3661 gactgcggcg cttcggcgga ccgttcctcg ccggcgccga attcaccgcg gccgatgcgt
     3721 tctacgcgcc ggtcgccttc cgggtgcaga cctattcgcc gccgctcgat gccacggcga
     3781 acgcctacgc gcagcgcctg cgggcgctcc cgtcgatgcg ggactggtac gcctcggccc
     3841 tcgcggaaac ctggcgcgac gcagcccacg aagccgaagt ggcgaactgg ggcacgatca
     3901 ccgaggacct gcgcgccgct tgaagcggcc cgccatcacg gcagttcgcc cgcgctgtgc
     3961 gggtcgtcgc tgtacgggtg catcgccgcc aggaagccgg gttgtgcttc cacccgtgcg
     4021 atccaggcgc gaaaatgcgg atacggatcc agcgtgattc ccgcttcttc cgcgcggctg
     4081 gcgtacgcga acaccgacat gtcggcgatg ctgtagcggt cgttggcgag gaacggacgg
     4141 gcggcgagtt cccgctccag gatcccgagc gtgtgcagcg cacccgtgcg tttcatctcc
     4201 accagcgcgg gaggtcgctg agccagcttg ccggtgaggg tccagtagcg cagcgagccg
     4261 atcaccgatt ccacgcgctc ctgttcgaag tgcagccact gctggacctt ggcccgctcg
     4321 aacggaccgg acggcagata tggcgtgccg tcggcaagat aagcgaggat cgcgttggat
     4381 tccgcgagcg cacgcccgtc ctccagccgg atcgcgggca cggtgccggt gggactgatg
     4441 gcgagatagt cgtcgctgcg tcctttgcct tcgaaaatgc tgacgatctc cgtccggtag
     4501 gggcgatcga ggtggtgcag cagaagccgc accttccagg cgttttgcga agcggggtag
     4561 tcgtacaggg tcagcatggc gggctcctgg tggcgagcgc gcagtctcca tcccggcggc
     4621 tcggtgcgcc atccgattct tgcgccgaaa tccctttcgc cgtcaaaaac gaccagcgaa
     4681 gcgatcggtc gcttcgacca gcttgtcgac gatcgccggc tccgacgcgg catgcccgga
     4741 cagcacgatg tggaactccg cctccggcca ggcctggtgc aggtcgaacg cgttcttggc
     4801 ggggcagatg acgtcgtagc gaccatgcac gatcacgccg ggaatgtggg gaatgcggtc
     4861 gatgtcgcgc agcaactggc ccggttccag gaacacctgg ttgcggaaat agtgcgcctc
     4921 gatccgcgcc aggctgaccg cgactcctgt cgccgaaaat tcggcttcgg tctcggggct
     4981 ctcggccaac gtgagcgccg acccttccca cgaaccccag gcgcgcgccg ccgccacgcg
     5041 aacagcctcg tcgccggacg tcaggcgctt ccagtaggcc tccagcatct gcccgcgttc
     5101 cgcaggcgga atcgcggata cataacgctg ccacttgtcc ggaaggatcc gcgacgcgcc
     5161 gccatcttcg tagaaccagc ggatttcctc cggccggccg aggaaaatcc cgcgcaatac
     5221 ca
//
LOCUS       DASSNH010000086         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_799538, whole genome shotgun sequence.
ACCESSION   DASSNH010000086 DASSNH010000000
VERSION     DASSNH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799538"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFK00_03025"
     CDS             complement(<1..223)
                     /locus_tag="VFK00_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7591958.1"
                     /translation="MSRSILAAALLATSACSFAAVGPNIPAVQPDHLNDYWVMTNTSL
                     NVDVPNSGVNLDKPTCSAVTYMIGSDGVTR"
     gene            complement(277..1740)
                     /gene="pyk"
                     /locus_tag="VFK00_03030"
     CDS             complement(277..1740)
                     /gene="pyk"
                     /locus_tag="VFK00_03030"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7591959.1"
                     /translation="MSVSRRTRIVATLGPATDAPGMLERTLGEGVDVIRLNLSHGKPE
                     DHLRRAREALAAAAKLGREVAVLADLQGPKIRVERFAEGPVQLKPGAAFTLDCRPDAP
                     AGDESRVGVSYAGLPDDVHEGDHLLLDDGLIELEVINVEGSQIHCRVVIGGKLSDRKG
                     LNRRGGGLSLDALSDKDRADIKLAAELKADFLAVSFVRSAADIHEARKLLREAGGNAW
                     LVAKIERAEALSALEEIIDASDAVMVARGDLGVEIGDAELPGLQKKIIRESLARNRVV
                     ITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSQESAAGKHPAAAVAAMSRIC
                     LGAERQFEPADEISVLTHRLDRTDQAIAMAATFLATQLPIRAIVALTESGTTAQWLSR
                     YRTSVPIYALTPSSQTRCRMLLLRDVKPMAFDGEGAYPALVVRDAVRQLFESGDLAVG
                     DRVLLTHGDHTGQGGGTNTMKLLSVGEDGIAESLRDL"
     gene            complement(1919..2461)
                     /gene="ppa"
                     /locus_tag="VFK00_03035"
     CDS             complement(1919..2461)
                     /gene="ppa"
                     /locus_tag="VFK00_03035"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7591960.1"
                     /translation="MDLVPAGRDVPDDINVVIEIPKDAEPVKYEVDKASGAIFVDRVL
                     TTPMRYPCNYGYIPNTLSGDGDPADALVLLPMPLTPGSVIRCRAVGLLNMDDEAGVDT
                     KLVVAPGEKVFAGYAHIRDIGDVPPHWRERIAYFFEHYKDLEAGKWVKVSGWADATAA
                     REEINAAVERYRQAPEKPNF"
BASE COUNT          383 a          809 c          828 g          442 t
ORIGIN      
        1 cgcgcgtcac gccgtccgag ccgatcatgt acgtgacggc ggagcaggtg ggcttgtcca
       61 ggttcacccc gctgttgggc acgtcgacgt tcagcgacgt gttggtcatc acccagtaat
      121 cgttgaggtg gtcgggctgc accgccggga tgttgggccc gaccgcggcg aagctgcacg
      181 cgctggtagc cagcagggcg gcggcaagga tggatctgct catgagtgga acaccacgaa
      241 tcgatgaatg ttggttttcg cgtcgccggg tccgcttcac aaatcgcgca agctctcggc
      301 tatcccgtcc tcgccaacgc tgagcagctt catggtgttg gtgccgccac cctggccggt
      361 gtggtcgccg tgggtcagca gcacccggtc gccgacggcg agatcgccgc tttcgaacaa
      421 ctggcgcacc gcgtcacgca ccaccagcgc cggataggcg ccctcgccgt cgaaggccat
      481 cggtttgacg tcgcgcaaca gcagcatccg gcagcgcgtt tgcgaactgg gggtcagggc
      541 gtagatcggc accgaggtgc ggtagcgtga cagccattgc gcggtcgtgc cggattcggt
      601 cagcgcgacg atcgcgcgta tcggcaactg ggtggcgagg aacgtcgcgg ccatcgcgat
      661 cgcctggtcg gtacggtcga ggcgatgcgt gagcacggaa atttcgtcgg cgggctcgaa
      721 ctggcgttcc gcaccgaggc agatccggct catcgcggcg acggcggcgg cagggtgctt
      781 gccggctgcg gattcctgcg acagcatcac ggcatcggtg ccgtcgatca ctgcgttggc
      841 cacatccagc acctcggcac gcgtcgggat cggcgattcg accatcgact gaagcatctg
      901 cgtcgcggtg atcaccaccc ggttgcgcgc cagcgactct cggatgatct tcttctgcag
      961 gcccggcaac tcggcgtcgc cgatttcgac gccgagatcg ccgcgcgcga ccatcacggc
     1021 gtccgaggcg tcgatgattt cctcgagtgc ggagagggct tcggcgcgct cgatcttggc
     1081 gaccagccag gcgttgccgc cggcctcgcg cagcagcttg cgcgcttcat ggatgtcggc
     1141 cgccgagcgc acgaatgaga cagcgaggaa gtccgccttc agttccgcgg ccagcttgat
     1201 gtcggcacgg tccttgtccg aaagcgcgtc cagcgaaagc ccgccgcccc tgcgattcag
     1261 gcctttgcga tcggacagct tgccgccgat cacgacccgg cagtggatct gggagccttc
     1321 cacgttgatc acttcgagct cgatcaaccc gtcgtccagc agcaggtggt cgccctcgtg
     1381 cacatcgtcg ggaagcccgg catagctgac ccccacccgc gattcgtcgc ctgcgggcgc
     1441 gtccggccgg cagtcgaggg tgaaggcggc gccgggcttc aactgcaccg gaccttcggc
     1501 gaagcgctcg acacggatct tggggccttg caggtctgcc aggaccgcga cttcgcggcc
     1561 gagcttggcc gccgcggcca gcgcttcgcg ggcgcgccga agatggtctt ccggcttgcc
     1621 gtgcgaaagg ttgaggcgga tgacgtcgac gccttcgccg agcgtgcgct ccagcatgcc
     1681 gggggcgtcg gtggcggggc cgagggtggc gacgatgcgc gtgcggcggg aaacgctcat
     1741 ccgactacgc tagcacattt gcttgtgcga tcgtccctga tcgctgcccc atggaacctt
     1801 cctgcgcata tcgtgtggct cactttggaa cagtacctcg ggcatccatg ccctgacttc
     1861 tcacaacaac cgcttcgaac gcgacggcgc ggtccccaac cgcgggtctt ggttccaatc
     1921 agaagttcgg cttttcgggt gcctgccggt aacgctcaac cgctgcgttg atttcctcgc
     1981 gggcggccgt ggcatcggcc cagccggaga ccttcaccca cttgcccgcc tcgaggtcct
     2041 tgtagtgctc gaagaagtag gcgatgcgct cgcgccagtg cggcggcaca tcgccgatgt
     2101 cgcggatatg ggcatagccg gcgaacacct tttcgccggg cgcgaccacc agtttggtat
     2161 ccaccccggc ttcgtcgtcc atgttgagca agcccactgc ccggcagcgg atcaccgaac
     2221 ccggcgtcag cggcatcggc agcagcacca gcgcgtcggc aggatcgccg tcccccgaca
     2281 gcgtgttcgg gatgtagccg tagttgcacg ggtagcgcat cggcgtggtc agcacgcgat
     2341 ccacgaagat cgcgccggag gccttgtcca cctcgtactt gacgggttcg gcgtccttgg
     2401 ggatttcgat gacgacgttg atgtcgtccg gcacgtcgcg gccggcgggg acgagatcca
     2461 gg
//
LOCUS       DASSNH010000087         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1713458, whole genome shotgun sequence.
ACCESSION   DASSNH010000087 DASSNH010000000
VERSION     DASSNH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713458"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      192..360
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            477..1076
                     /locus_tag="VFK00_03040"
     CDS             477..1076
                     /locus_tag="VFK00_03040"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7591961.1"
                     /translation="MFTGIVQATGRVADAEKRGGDLRLIIEAPDFGCADIPPGDSISV
                     SGCCLTVTEAASGTLAFDVSNETLSLTTLGGLRAGDRVNLEPALRLSDRLGGHLVSGH
                     VDGVGTIVAIEADARSQRWTIEVPAALARYIAPKGSICVDGASLTVNAVAGKRFEVNL
                     IPHTVAATTFGERRMGDRVNLEVDLLARYLERLLANPEH"
     gene            1080..2231
                     /gene="ribB"
                     /locus_tag="VFK00_03045"
     CDS             1080..2231
                     /gene="ribB"
                     /locus_tag="VFK00_03045"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET7591962.1"
                     /translation="MAFATIPELLDEIAAGRMVVVLDDEDRENEGDLIMAADAVRPED
                     INFMAREGRGLICLALDPEHGRRLGLQPMVRENASPLHTNFAVSIEAARGVTTGISAH
                     DRAVTIRTAVAPDAKPSDLIQPGHVFPLLAQPGGVLTRAGHTEAAVDLAALAGRTPAG
                     VLVEVLREDGAMARRPDLETFAAKHGLKVGSVADLIRHRLATEKTVERVFDGEVATEF
                     GAFRLVAWRDAIRGDLHFALVRGAIGDGAPVLTRVQVRDTLSDVLHIEREDFGLAMTA
                     ALRRIAAEGRGVVVVLAGEEDPAALLLRLAGDAVAVPPPTGAGAQQEWRQLGLGAQIL
                     ADLGVRRLRVLGTPRKLVGLAGFDLEVVEYVGEPGPGTGGRGPEEGQVV"
     gene            2343..2825
                     /gene="ribH"
                     /locus_tag="VFK00_03050"
     CDS             2343..2825
                     /gene="ribH"
                     /locus_tag="VFK00_03050"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7591963.1"
                     /translation="MSNQRATPTEDLRSPSGARYAVLASRWNGEIVDALVDGASRAFA
                     EHGVEDGSIDVLRVPGAWELPVLARQLAASGRYAAIVALGCVVRGETRHYEHVADECA
                     RGLMRVALDSGVPVLNGVLAVEDEADAWARAGGARGNKGADVARAAIEMASTMIRSST
                     "
     gene            2822..3274
                     /gene="nusB"
                     /locus_tag="VFK00_03055"
     CDS             2822..3274
                     /gene="nusB"
                     /locus_tag="VFK00_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005910971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7591964.1"
                     /translation="MTPPGRRDGIDPAARTRARRRAVQALYAWELGGNPMRKVIDDFR
                     SEQDMQIADLEYFEDLLRGVDAHRAELDAGLAPLLDRDIARVDPIERAVLRVSAYELK
                     YRLDVPYRVVLNEAVEVAKRFGSEQGHTYVNGVLDKLAHAWRAVETGS"
BASE COUNT          514 a         1146 c         1234 g          539 t
ORIGIN      
        1 ctggcacggc cgctgttcgc gctgccaccg ctgcgcgcga tggactcgcg ctggcggttg
       61 cggacggtcg aagaacgcag ggtcggtgcg gacctgcggc tgctcctgcg cccggatccg
      121 ggcggcgaag ccgacgccca ccagcggccg cccgcggcgg tgtagacttg ccgcgccggc
      181 cagtccggct acgacgtctt cagggcgggg cgaaattccc caccggcggt gggtccgcaa
      241 ggacaagccc gcgagcgctc gcgacaccgt cgcggggtca gcagatccgg tcgaatgccg
      301 gagccgacgg tcggcggcag agggttgccg ccggagagtc cggataaagc gaagacggtc
      361 cggtgtccgt acggggcgcc ggcctgatgc cttggggcgt tctcgctgcc acttttgcgg
      421 agaacgttca tgaagctttc cgtttcccat tccacttttc gctggacgcc gtgctgatgt
      481 tcacgggcat cgtgcaggcg accggtcgcg tcgcggacgc ggaaaagcgc ggcggggatt
      541 tgcgcctgat catcgaggct ccggatttcg ggtgcgcaga cattccgcct ggcgacagca
      601 tcagcgtgtc ggggtgctgc ctcaccgtga cggaggccgc cagtggcaca ctcgcattcg
      661 acgtatccaa cgaaaccctt tcactgacca cgctgggcgg tctgcgcgcg ggcgatcgcg
      721 taaacctcga acccgcgctg cggctctccg atcggctggg cgggcacctg gtttcgggtc
      781 acgtcgatgg cgtcggcacc atcgtggcga tcgaagccga cgcgcgttcg caacgctgga
      841 ccatcgaggt gcccgcggca cttgcccgct acatcgcgcc caagggatca atctgcgtcg
      901 acggggccag cctgaccgtc aatgccgtcg cggggaagcg gttcgaggtc aacctgatcc
      961 cgcacaccgt tgccgcgacg actttcggcg aacgtcgcat gggcgatcgg gtgaacctcg
     1021 aagtcgacct gttggcgcgt tacctcgaac gattgctggc gaatccggag cactgaacca
     1081 tggccttcgc aaccattccc gaactgctcg atgagatcgc cgccggccgc atggtggtgg
     1141 tgcttgacga cgaggaccgc gagaacgagg gcgacctcat catggccgcc gacgcggtgc
     1201 ggcccgagga catcaacttc atggcgcgcg aagggcgcgg gttgatctgc ctggcgctcg
     1261 atccggaaca cggccgcagg ctcgggctcc agccgatggt gcgcgagaac gcctcgccgc
     1321 tgcacaccaa cttcgcggtg tcgatcgaag ccgcacgtgg cgtcaccacc ggcatttccg
     1381 cacacgaccg cgccgtgacg atccgcaccg cggtggcgcc ggatgccaaa ccatccgacc
     1441 tgatccagcc cggccacgtg ttcccgttgc tggcccagcc gggcggcgtg ctgacccgcg
     1501 ccgggcatac ggaggccgcg gtcgacctgg cggcactggc tggccgcacg ccggccgggg
     1561 ttctggtcga ggtcctgcgc gaggacggcg ccatggcccg gcgtcccgat ctcgaaacgt
     1621 ttgccgcgaa acacggactc aaggtcggca gcgtcgccga cctcatccgc caccgcctcg
     1681 ccaccgagaa aaccgtcgag cgcgtattcg atggcgaggt cgccaccgaa ttcggtgcat
     1741 tccgcctggt ggcgtggcgt gacgcgatcc gcggcgacct gcatttcgcg ctggtgcggg
     1801 gcgccatcgg cgacggcgcc ccggtgctga cgcgcgtgca ggtccgcgac accttgtcgg
     1861 acgtgctgca catcgaacgc gaggacttcg gcctggcgat gaccgcggcg ttgcggcgga
     1921 tcgcggccga gggccgcggc gtggtcgtgg tgctcgcggg cgaggaggat cccgcggcat
     1981 tgttgttgcg cctggccggc gatgcggtcg cggtcccgcc gccgaccggc gccggtgcgc
     2041 aacaggaatg gcggcaactc ggcctgggtg cgcagatcct tgccgacctc ggggtgcggc
     2101 gcctgcgcgt gctcggcacg ccgcgcaagc tggtggggct ggccggtttc gacctcgagg
     2161 tggtggagta cgtgggagag ccgggaccgg ggaccggggg ccggggaccg gaggaagggc
     2221 aagtcgttta ggacgcatcg ccatgcacta ttcgtccgcc agaatacgca tcccgctact
     2281 gttacgcttt tccggtcccc ggtccccggt ccccggccct ccctgtccca gcccgcatcc
     2341 cgatgtccaa ccagcgcgcc acgcccactg aagacttgcg ttcgccgtcc ggcgcccgct
     2401 acgccgtgct cgcgagtcgc tggaacggcg agatcgtgga tgcgctggtc gatggggcaa
     2461 gccgcgcttt cgcggaacac ggcgtggaag atggatcgat cgatgtgctg cgggtgcccg
     2521 gcgcgtggga attgccggtg cttgcgcggc agctcgcggc gagtggccgt tacgccgcga
     2581 tcgtcgcgct gggatgcgtg gtgcgcggag agacccgcca ctacgaacac gtggccgacg
     2641 aatgcgcgcg cgggttgatg cgcgtcgcgc tcgacagcgg ggtgccggta ttgaacggtg
     2701 tccttgcggt cgaggacgaa gccgacgcgt gggcgcgcgc cggcggggcg cgcggcaaca
     2761 agggtgcgga cgtggccagg gccgcgatcg agatggcttc gacgatgatc aggtcatcga
     2821 catgacgccg cccggccgcc gcgacggcat cgatcccgcc gcacgcacgc gggcgcgccg
     2881 gcgtgcggtg caggccctgt acgcgtggga gctcgggggc aacccgatgc gcaaggtcat
     2941 cgacgacttc cggtccgagc aggacatgca gatcgccgac ctcgaatatt tcgaggacct
     3001 gctgcggggc gtggatgcgc atcgcgccga gctggatgcc gggctggcac cactgctcga
     3061 tcgcgacatc gcgcgggtcg atccgatcga gcgggcagtg cttagggtgt cggcctacga
     3121 attgaagtac cggctcgacg tgccgtatcg cgtggtcctg aacgaggcgg tcgaagtggc
     3181 caagcgcttc ggttccgagc agggccacac ctacgtcaac ggcgtgctgg acaagttggc
     3241 gcacgcctgg cgcgccgtag aaaccggaag ctgaatcgtg gatgcccccg gccgaccggc
     3301 attcgtcgcc gcgccggaaa tccggaccgg gcgcctgcgc ctgcgcgcgc atcgcgccgg
     3361 cgatttcgat gcgtgccgcg cgatctggtc cgatcccgaa gtcgtgcgcc acatcatcgg
     3421 caagccctcc acg
//
LOCUS       DASSNH010000088         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1678460, whole genome shotgun sequence.
ACCESSION   DASSNH010000088 DASSNH010000000
VERSION     DASSNH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678460"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFK00_03060"
     CDS             <1..138
                     /locus_tag="VFK00_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET7591965.1"
                     /translation="SGDDLFVHFRAIQGDGFKSLQEGQKVTFIAVKGPKGMQADQVQA
                     A"
     gene            443..2587
                     /locus_tag="VFK00_03065"
     CDS             443..2587
                     /locus_tag="VFK00_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET7591966.1"
                     /translation="MNPGLSPDQRAELVLKAMTQDEKFRLVRTEFGDGDHKPAGALGS
                     AGYQPAITRLGLPAIQESDAGLGVAKPLDLGAIALPSGLATAASFDPALAYAGGAMIG
                     NEAHRRGFNVMLAGGADLVRDPRNGRNFEYAGEDPLLAGVIVGNAVAGIQSRHLVSTV
                     KHYALNDLETARNTLSANIDRAAARESDLLAFEIAIETGHPGAVMCAYNRINSRYACE
                     NDWLLDQVLKHDWHYQGFVMSDWGAVHGATKSALAGLDQESAGETFDKEVYFDKPLRA
                     AVASGEVPQSRIDDMARRILRSLFAAGVIDHPAKPGPIDFDADRKVARRVEEAGAVLL
                     RNRGSLLPLSAAQSVAVIGGHADKGVLTGGGSSAVQSPGGNAVPGARPMNWPGPRIYQ
                     PSAPLAAIEKRAHGKVGFADGEGIAAAAQLAARSDVAVVFVEQWAAESFDLPTLALPG
                     NQDALVEAVAKANPHTIVVLETNGPVAMPWLAQVGAVLEAWYPGAAGGEAIARLLYGE
                     VAPSGRLPVSWPRDESQLPRPQIPGAGLAALGLPPQGRPAQDVDYDIEGADVGYRWYQ
                     RKHLEPLFPFGYGLAYTHFAYSGFEPHVEQGRVTASFTVTNRGEREGVDVPQLYVTLP
                     GADEVRRLAGWCRVSLKPGQSAHLTVTADPRLLVNFDSHGQRWQRPAGVYLLQLGHSA
                     TSFQGQGKATLTAASWPADAAPTGTPQPCANP"
BASE COUNT          472 a          947 c          897 g          418 t
ORIGIN      
        1 agtggtgacg acttgttcgt ccacttccgc gccatccagg gcgacggttt caagtcgctg
       61 caggaaggcc agaaggtgac cttcatcgcc gtcaagggcc cgaagggcat gcaggcggat
      121 caggtgcagg cggcataagc ctccgtaccc ggcttgaggg aaagcccgct tcggcgggct
      181 ttttcatgtc cggcctccga cgagcgccgg acgcgtcgcg cccatcctgt cctgccgggc
      241 gcagacggga tcgagtgccc ccgcaaacca aaaatgatct aaggttcgca atgcgaggac
      301 cgggctgctc gaacgagaaa tgaaactgcc gtctttcatc aaggcatccg tgttgcacgc
      361 gccactcgcc gcgctcgcat gcctcgtagc cacggcaagt ttcgcggcaa cggcagcggg
      421 ccgggatgca gcacacccat ggatgaatcc cggcctctcg cccgaccagc gcgccgagct
      481 ggtgttgaag gcaatgacgc aggacgaaaa gttccggctg gttcgcaccg aattcggcga
      541 tggtgaccac aagccagcgg gcgcgctcgg ctcagccggt taccagcccg cgatcactcg
      601 cctgggcctg cccgcgatcc aggaaagcga cgccggactc ggcgttgcca agccgctcga
      661 cctcggtgcg atcgccctgc cctcggggct cgcgaccgcg gccagcttcg atccggcgct
      721 cgcttacgcc ggcggcgcga tgatcggcaa tgaagcgcat cgccgcggct tcaacgtgat
      781 gctggcgggc ggtgcagacc tggtgcgcga cccacgcaac ggccgcaatt tcgaatacgc
      841 aggcgaagat ccgctgctgg ccggcgtgat cgtgggcaac gccgttgccg gcatccagag
      901 ccggcacctc gtctccaccg tcaagcatta cgcgctcaac gacctggaaa ccgcccgcaa
      961 cacgctgtcc gccaacatcg atcgtgccgc ggcgcgcgag tccgacctgc tggccttcga
     1021 aatcgccatc gaaaccggtc atccaggcgc ggtgatgtgc gcctacaacc gcatcaattc
     1081 ccggtacgcc tgcgaaaacg actggctgct cgaccaggtg ctgaagcacg actggcacta
     1141 ccagggcttc gtgatgtcgg actggggcgc cgtgcacggc gccacgaaat ccgcgctggc
     1201 cggactggac caggaatcgg ccggagaaac attcgacaag gaggtgtact tcgacaagcc
     1261 gttgcgagcc gcggtggctt ccggcgaggt gccgcaatcg cgcatcgacg acatggcgcg
     1321 gcgcatcctg cgcagcctgt tcgcggccgg cgtgatcgac catccggcca aacccggacc
     1381 catcgacttc gatgccgacc gcaaggtggc ccggcgcgtc gaggaagccg gtgcggtgct
     1441 attgcgaaac cgcggttcgc tgctgccgct gtccgccgcg caatccgtgg cggtgatcgg
     1501 cggccatgcc gacaagggcg tgctgaccgg gggcggatcc tccgcggtgc agtcgcccgg
     1561 gggcaacgca gtgccggggg caaggcccat gaattggccg gggccgcgca tctaccagcc
     1621 ttccgcgccg ctcgcggcca ttgaaaagcg cgcgcacggc aaggttggct tcgccgatgg
     1681 cgagggcatc gcggccgccg cgcaactggc tgcccgctcg gacgtcgcgg tggtattcgt
     1741 cgaacaatgg gcggcggaaa gtttcgacct gccaacgctc gccctgcccg gcaaccagga
     1801 cgcactggtc gaagccgtgg cgaaggcgaa cccgcacacg atcgtcgtcc tggaaaccaa
     1861 tggcccggtt gcaatgccgt ggctcgccca ggtcggggcg gtgctggaag cgtggtatcc
     1921 cggcgcggcc ggcggcgagg caatcgcgcg gctgctgtac ggcgaagtcg ccccaagcgg
     1981 acgcctgccg gtgagctggc cgcgcgacga atcgcaactg cctcgcccgc aaatccccgg
     2041 cgccggtctc gctgcccttg gcctgccgcc gcaaggcagg ccggcgcagg acgtggacta
     2101 cgacatcgaa ggcgctgacg tcggttaccg ctggtaccag cgcaagcatc tggaaccgct
     2161 gttcccgttc ggctacggcc tcgcctacac gcactttgca tacagcggct tcgaaccgca
     2221 cgtcgagcag ggccgcgtca cggcctcgtt caccgtcacc aaccgcggcg aacgcgaagg
     2281 cgtggacgtg ccgcaactct acgtgaccct gcccggtgcg gatgaagtgc ggcgactggc
     2341 aggctggtgc cgcgtcagcc tcaagcccgg ccagtccgcg cacttgacgg tcaccgccga
     2401 tccgcggttg ctcgtgaact tcgacagcca tggccagcgc tggcagcgac cggccggcgt
     2461 ctatctattg cagttgggcc attcggcaac ctcgttccag ggtcagggaa aggcgacgct
     2521 gaccgccgcc tcctggcccg ccgatgcggc gccaaccggc acgccgcaac cctgcgccaa
     2581 cccctagcgt cgggccgagg caacctcgag ccggtgcagg cgaattcgtt gccgtcaacc
     2641 ggaaagacaa catgcccgtc tcgttcaccc gccgcgagtt catcaagtcc agctccctgt
     2701 tcgggctttc cgtcgccctg atgggtccgg cgag
//
LOCUS       DASSNH010000089        12216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1297118, whole genome shotgun sequence.
ACCESSION   DASSNH010000089 DASSNH010000000
VERSION     DASSNH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297118"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="bla"
                     /locus_tag="VFK00_03070"
     CDS             <1..727
                     /gene="bla"
                     /locus_tag="VFK00_03070"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HET7591967.1"
                     /translation="GVFLVTAPTGDVLIDGGVPGEAALIEANIRSLGIDLRDIKWILN
                     SHAHSDHAGGIARLARDTGAQVIAGAGDAALLERGGHDDPQYGDRFPFPPVHVARAAA
                     DGERLHLGDLVLTAHSTPGHTQGNTTWTWMSCEGTRCLHLVDVGSLSAPGFRLVGKPN
                     SPAIVGEFEHSFSVVAALPCDIPLAPHPGMVDFWTRVAERGQGHPDALVDPAGCRAYA
                     NGARESFEAQLAKQRAEAAPATK"
     gene            complement(801..2114)
                     /locus_tag="VFK00_03075"
     CDS             complement(801..2114)
                     /locus_tag="VFK00_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7591968.1"
                     /translation="MNTASPVARTSAPKSLFANRDFGLLFGGSSVSAFGDQFTLVALP
                     WLVLKLTGNPAALGLVLAVMAFPRAAFMLIGGAVVDRMSPRRVLLGARAANAACIVVL
                     AALVLIGDIQMWMVYLLALGIGLSTAFAYPAGSAILPQLVPLEQLRPANAAFMGMRQL
                     SMIGGPVLAGFVIALGAHHQVSTQSLPDGHGLGLAFGIDAASFVFSLVSLAMIRIHGD
                     THPPGKKTGVFASIAAGVRYVWADVPLRAFVIYASFVGVFVGGPIQVGLPVLADTRLN
                     LGAASLGILMTAQGVGMFIGSFLSGYAQRAARGHLGVMVLCFDGMTGLVLAALTLVHT
                     TYFGAALLFCTGLLGGVVMIAIFTWIQQRVSQPMMGRTMSILMFSFMGLAPLSAAAAG
                     ALLKVISLTALFGIAGLTLTAIALCCLASPQMRSIRAMHSEAAKA"
     gene            2421..2789
                     /locus_tag="VFK00_03080"
     CDS             2421..2789
                     /locus_tag="VFK00_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7591969.1"
                     /translation="MNIEQIAQRLVELCRKGEYEQAQRELYAQNAVSIEMPGLPDGAL
                     GDVEGLEAIYEKGRQFSERVEAVHATEASDPMIAGNWFSVAFSMDMTFKGRGRVQMQE
                     IGVYHVRDGKIDREQFFYDM"
     gene            2912..3685
                     /locus_tag="VFK00_03085"
     CDS             2912..3685
                     /locus_tag="VFK00_03085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7591970.1"
                     /translation="MKTSNAIAASATLNDPRWQRVLARDATADGEFFYSVRTSGVYCR
                     PSCASRRARPEHVAFHPTAEDAQHAGFRPCKRCKPDRLAVDAHVAADDIRYAVAQSSL
                     GQVLVATSQGGVCAILLGDDSGAPKSGLRERFPVARLGEDTAGLEAIAAQVVGLIEAP
                     VAAQFDLPLDARGTPFQRRVWQALRDINAGSTASYAQIAARTGAPKSARAVAQACSAN
                     PLAVAIPCHRVVRGDGALSGYRWGVQRKRVLLQREGYAA"
     gene            3682..4395
                     /locus_tag="VFK00_03090"
     CDS             3682..4395
                     /locus_tag="VFK00_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7591971.1"
                     /translation="MTSATKPPPAPVDWLRVEADLDADGAAVTEKLLAPEGCRELAGL
                     YAEDDRFRSRVVMARHGFGRGEYKYFDHPLPRSVADLRKALYPPLARIANRWNEALRL
                     DVRYPSTHAEYLDRCHAAGQCKPTPLLLSYGEGDYNCLHQDLYGEHVFPLQLTVLLSQ
                     PGKDFEGGEFVLTEQRPRMQSRVEVVPLRQGDAVIFPVHHRPVQGARGVYRVNLRHGV
                     SRVHSGKRHTLGIIFHDAA"
     gene            complement(4516..5949)
                     /locus_tag="VFK00_03095"
     CDS             complement(4516..5949)
                     /locus_tag="VFK00_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7591972.1"
                     /translation="MECRVAMGLLLGVGVAVAGFAQTPAAQDWPMFGGNVQSTSANPQ
                     PTGITAANVGKLTRRQVELPGTVDASAIYLHGVAIHGARHDAIFVTTTYGKTLATDAT
                     SGAILWTYTPASYSKLAGTRQITNSTPVADPDRQFIYAASPDGYIQKLAIGDGHAVWR
                     TAITRLAHREKMDSPLKFFRGHVIAVTAGYIGDAPPYQGHVAILDGKSGQLLHVWNSL
                     CSDRTVLMEPSSCPQSDSAIWGRAGAVIDPDSGHIFVATGNADWDGKTNWGDSLVELD
                     ADATRMLGNYTPANTGHLNEDDLDLGSTSPVLLGNGLIAQGGKDSKIVLLSLEAIAGT
                     TPHRGGELQTVATPDGASLFTQPAVWKHAGQTWMFVGDERGTEAWQLQGGKLRRMWSN
                     GTDGTSPFEADGLLYVYAPNGGLNVYEAASGTRITTLACGAGHWNSPIVVDGRIILPE
                     GNANDHATRGVLDILSVVAQPARGAGN"
     gene            6056..6664
                     /gene="lexA"
                     /locus_tag="VFK00_03100"
     CDS             6056..6664
                     /gene="lexA"
                     /locus_tag="VFK00_03100"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET7591973.1"
                     /translation="MDELTDRQREILDFIARRIGEGLPPTLVEIAGAFGFNQPRAAWK
                     HLQALQNKGCIELLPGRVRGIRLVADAPRAKPAGLPLVGRVAAGRPILAQEHIERHVA
                     VDARLFSPRAHFLLRVEGDSMQGVGILDGDLVAVHRTPEARDGQIVVARIEDEVTVKR
                     LRIRRQRVQLHAENPAYPPIEVDPRRTTFAIEGLYVGVIRVD"
     gene            6666..7280
                     /gene="imuA"
                     /locus_tag="VFK00_03105"
                     /pseudo
     CDS             6666..7280
                     /gene="imuA"
                     /locus_tag="VFK00_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467237.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
     gene            7461..8873
                     /locus_tag="VFK00_03110"
     CDS             7461..8873
                     /locus_tag="VFK00_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET7591974.1"
                     /translation="MRWACILFPQLALDDVLRRRADRDAPLVLIDPAQSRPLVRAVNR
                     RAGAAGVKVGQSLAVARALCDGLVVADHDDAAAERCREFLAAWAYAYSSEVSRDFPDA
                     LLLEVEASFRLFGGWPNLQARLRLELDELGFRHRIALAPNPHAARVLAGVHDGIAIPD
                     AILRNALGALPVEQAGLEPECAMGLRRMGLRKLRQIFALPRPELGRRIGKDALAHLDR
                     LTGALAAPLTFYRPPDAFDACIELPFNTASSESLLFPLRRLVRDFASFLAGRDGGVQC
                     FTLTFEHEHDARTRIPIGLLSPERDPSLLFDIARERLDRTSLCAPVCALRLQADELPP
                     FAPSRRDLFDPRTQQAEPWEALRERLRSRLGDEAVHGIALHADHRPERAWQVEHGKPR
                     VDEPPRHPRPAWLLQRPIPLRPAVRQILAGPERIESGWWDGQDVRRDYYVVETGAGQR
                     AWVFRPAGETGAFMLHGWFA"
     gene            9065..12166
                     /locus_tag="VFK00_03115"
     CDS             9065..12166
                     /locus_tag="VFK00_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET7591975.1"
                     /translation="MDRRILPPYAELHCLSAFSFQRGASVARELFERAKRLGYRALAI
                     TDECSLAGIVRALEASRKTGVRLIVGSEMQVQDGPKLVLLAQTQAGYSRLCSLITLAR
                     RRADKGEYRSLREDFAEGCNDDLLALWVPGADIDETHGAWVRDLFPERAWLAVELHRG
                     PDDATRLAQLRDAGARLGLPLVAAGDAHMHVRSRRALQDVLTAVRHRTTIAEAGHRLY
                     PNGERHLRTPEALAAIYPHALLEETLRIADLCTFRLEELRYEYPHELVPAGHTPTSWL
                     RELTEAGARRRWKGGVPDKVRALIEHELALIAELRYESYFLTVHDIVQFARSSGILCQ
                     GRGSAANSAVCYALAVTEIDPARMNMLFERFLSKERNEPPDIDIDFEHERREEVIQYV
                     FDRYGRDRAALTAVASSYRGRGAIRDVAKALGLPADLVDRLARCMDHWGGAESLGATL
                     RERGFDPDSGTLRKVVALTVQLIGMPRHLSQHPGGFVMSEHPLHTLVPVENAAMEDRT
                     VIQWDKDDLDVLGLLKVDCLALGMLTAVRKTLELLCASGRRDMKRLADIPSKDPDVYA
                     MIGRADTIGVFQIESRAQMAMLPRLRPKCFYDLVIEVAIVRPGPIVGDMVHPYLRRRN
                     GEEKVTYPSDALQAVLERTLGVPLFQEQVMQIAIAAAGYSPGEADELRRSMAAWKRHG
                     GLEPHRDRLAAGMRARGYSQAYADHLFEQIKGFGSYGFPESHAASFALITWVSCWLKF
                     HEPAAFACGVINSQPMGFYSPDQLLQDARRHGIRVLPVDVRYSGWDCSLEDRSPGSAQ
                     PAIRMGFRMIRGFREDAAAAIVAARAARAFTDVADLCARASLDVRQRSLLADAAALRG
                     LAGHRHRARWTVAGVETSPPLFIDAPATKETAITLPLPTRAEEVRTDYAMTGTTLGAH
                     PLSFLRATLSRRRCRRSAELKRCPNGQQVRTAGLVTLRQRPQTASGVIFMTLEDEDGM
                     TNVVVWNDLAQRQRRELLESRLLAVEGRLENRHGVQHLIAERLHDWTRLLGALDTRSR
                     DFH"
BASE COUNT         1979 a         4035 c         4194 g         2008 t
ORIGIN      
        1 cggcgtcttc ctcgtcaccg caccgaccgg cgatgttctg atcgacggcg gcgtgcccgg
       61 ggaggctgcg ctgatcgaag cgaacatccg aagtctcggc atcgacctgc gcgacatcaa
      121 atggatcctc aattcgcacg cgcattccga ccatgcgggc ggcatcgcgc gactcgcgcg
      181 cgacacgggc gcgcaggtga tcgcgggtgc cggggatgcc gcattgctgg agcgcggcgg
      241 tcacgatgat ccgcagtatg gcgaccggtt tccgttcccg ccggtgcacg tggcgcgcgc
      301 ggcagcggat ggtgaacgct tgcacctggg cgacctggtg ctgacggcgc attcgacgcc
      361 cggccatacc caaggcaaca ccacgtggac atggatgtca tgcgagggca cgcgctgcct
      421 gcacctggtc gacgtcggca gcctgtcggc gcctggcttc cgattggtcg gaaagccgaa
      481 ttccccggcg atcgtcgggg aattcgaaca cagcttttcc gtggtcgcgg ccctgccttg
      541 cgacatcccg ctcgctccgc atcccggcat ggtggatttc tggacgcgcg tggccgagcg
      601 tgggcagggc catcccgatg cactggtcga tcccgccgga tgccgcgctt acgcgaacgg
      661 cgcgcgcgaa agtttcgagg cgcaattggc gaagcagcgc gcggaggcag ctcctgcaac
      721 caagtgatcg ccggggagga ctttgttgtc tacatcggcg ggttatgccg ccgcgcggcc
      781 aatccgacct gcttcaacgt tcaagccttc gccgcctccg agtgcatcgc ccggatgctc
      841 cgcatctgcg ggctcgcgag gcagcacagc gcgatcgcgg tgagggtgag gccggcgatg
      901 ccgaacaacg cagtcagcga gatcactttc agcagcgcgc cggcggcggc ggccgagagc
      961 ggcgcgaggc ccatgaacga gaacatcagg atgctcatgg tgcggcccat catcggctgc
     1021 gagacgcgct gctggatcca ggtgaagatc gcgatcatca ccacgccacc gaggagtccg
     1081 gtgcagaaca gcaaggccgc gccgaagtag gtggtgtgca ccagcgtcag cgcggcaagc
     1141 accaggcccg tcattccatc gaaacacagc accatcacgc ccaggtgccc gcgcgccgca
     1201 cgctgggcgt agcctgaaag aaagctgccg atgaacatcc cgacgccctg cgcggtcatc
     1261 aggatgccca gcgacgccgc acccaagttc agtcgagtgt ccgccagcac cggaaggccc
     1321 acctggatcg gcccgcccac gaacaccccg acgaagctcg cgtagatcac gaatgcgcgc
     1381 agcggcacgt ccgcccacac gtagcgcacg cccgcggcga tgctcgcgaa cacgcccgtt
     1441 ttcttcccgg gcgggtgggt gtcgccgtgg atgcggatca tggccagcga aaccagcgag
     1501 aacacgaaac tcgccgcatc gatgccgaag gccagcccga gtccgtgtcc gtcaggcagt
     1561 gattgcgtgg aaacctgatg gtgcgcgccc agcgcgatca cgaaaccggc gagcaccggt
     1621 ccgccgatca tgctgagctg gcgcatgccc atgaacgccg cgttcgccgg gcgcaactgc
     1681 tcgagcggca ccagttgcgg caggatcgcg gaaccggcgg gatacgcgaa cgcggtggac
     1741 aggccgatgc cgagcgccag caggtagacc atccacattt gtatgtcgcc gatcagcacc
     1801 agcgcggcga gcaccacgat gcaggcggcg ttggccgcac gcgcgccgag cagcacgcgc
     1861 cgcggcgaca tccggtccac caccgcgcca ccgatcagca tgaaagctgc gcgcgggaac
     1921 gccatcaccg ccagcaccag gcccagcgcg gcggggttgc cggtgagttt caggaccagc
     1981 catggcagcg ccaccagcgt gaactgatcg ccgaacgccg aaaccgaact gccgccgaac
     2041 agcaggccaa aatcgcggtt ggcaaaaagg gatttaggcg cggacgtccg tgccacgggg
     2101 gaggcggtgt tcacggaggt cctcgccgaa atacgccgtg cagtttaccg tcgcggaaac
     2161 ctcaccgcgg ccgtggatga tcgcgccgcg ccgggcaccc cgcacctaca ctctgttcgc
     2221 cggcgctccc ggttgcgacg gacgcggtgg aagtgactcg aaccatccga tcaccgaacg
     2281 cggccaagta cgccatccac tggaagacgc cccgtggcgc aggtgaaccg gattggctcc
     2341 gcatcgctgc aatcgacgct ccgcggcggt gtggcgccgg cggggcctgc tcctacaatc
     2401 atcatttaca ggagtacatc atgaacatcg aacagattgc ccaacgcctg gtcgagttgt
     2461 gccgcaaggg cgaatacgaa caggcgcagc gcgagttgta cgcgcagaac gcggtcagca
     2521 tcgaaatgcc gggcttgccg gatggcgcgc ttggcgatgt cgaagggctg gaggccatct
     2581 acgagaaggg ccgccagttt tccgagaggg tggaggcggt gcacgccacc gaggcgagcg
     2641 atccgatgat cgcgggcaac tggttcagcg tggccttttc catggacatg accttcaagg
     2701 gtcgcggccg cgtgcagatg caggaaatcg gcgtgtacca cgtgcgcgac ggcaagatcg
     2761 accgcgagca gtttttctac gacatgtgaa ggaacggttg caaagcggtt gtgtttgcaa
     2821 tccattcgca ggcgccgaac gcaagaatcg gagtgtgccg gcggccgcct cgccctagat
     2881 tgcattccac gatccacgct ggaggaatgc aatgaagacg agcaatgcca tcgcggcttc
     2941 cgcgacactg aacgatccgc gctggcagcg cgtgctggcg cgcgatgcca cggccgatgg
     3001 cgagttcttt tattcggtca gaaccagcgg cgtgtactgc cggccctctt gtgcgtcacg
     3061 gcgtgcgagg cccgagcacg tcgcgtttca tcccaccgcc gaggacgcgc agcacgccgg
     3121 atttcgtcca tgcaaacgct gcaagcccga ccggctcgcg gtggatgcgc acgttgcagc
     3181 cgatgacatc cgctatgcgg tcgcgcaaag ttcgctggga caggttctcg tcgccaccag
     3241 ccaaggcggg gtctgcgcga tcctgctcgg tgacgattcc ggcgcgccga aaagcggatt
     3301 gcgcgagcga ttccccgttg ccaggttggg cgaggacacg gccggactcg aggcgatcgc
     3361 ggcgcaggtg gtgggattga tcgaagcgcc cgtggccgcg caattcgact tgccgctgga
     3421 cgcccgaggt acgccgttcc agcggcgcgt atggcaggca ctgcgcgaca taaatgccgg
     3481 ctcgacggcg agttatgcgc agatcgccgc gcgcactggt gcgccgaaat cggcgcgtgc
     3541 ggtggcgcag gcctgctctg ccaatccgct ggcggtggca attccctgcc atcgcgtggt
     3601 gcgcggcgac ggcgcgctgt cgggttaccg ctggggcgtg cagcgcaagc gcgtgctgct
     3661 gcaacgcgaa ggttacgcag catgacctcc gcgacaaaac cacctccggc gccagtggac
     3721 tggctgcgcg tcgaagcgga tctggatgcc gatggcgcag cggtgaccga aaagctgctc
     3781 gcgccggaag gctgccgcga actggccggc ctgtatgccg aggacgatcg gttccgcagc
     3841 agggtggtga tggcacggca tgggttcggc cgcggcgaat acaagtattt cgatcatccg
     3901 ctgccgcgca gtgtcgccga tttgcgcaaa gcgctgtatc cgccgctggc gcgcatcgcc
     3961 aatcgctgga atgaagcgtt gcgcctggac gtgcgttacc cctcgaccca cgccgaatat
     4021 ctcgatcgtt gccacgctgc cggccagtgc aaaccgacgc cgctgctgct gagttatggc
     4081 gagggcgatt acaactgcct gcaccaggat ctgtacggcg aacacgtttt tccgctgcag
     4141 ctcacggtgc tgctgtcgca accgggaaaa gacttcgaag gcggcgaatt cgtgctgacc
     4201 gaacagcgtc ctcgcatgca gtcacgcgtc gaagtggtgc cgttgcgaca gggcgacgcg
     4261 gtgattttcc cggtccatca ccggccggtg cagggcgcgc gcggcgtgta ccgggtcaac
     4321 ctgcgtcacg gcgtcagccg cgtccacagt ggcaagcgcc acacgctcgg catcatcttc
     4381 catgacgcgg cgtgaccggt tcccgtgcag gttttggttc gccgtcagac gaaccgcctc
     4441 gacgttgttg ccgtcgggat cgagcgggaa agcagcgtag caagtgggac cgtagcattc
     4501 cgccagcggg gccactcaat ttccagcgcc tcgcgcgggc tgcgctacga cagacaggat
     4561 gtccagtacc ccgcgggtgg cgtggtcgtt ggcgttgcct tcgggcagga tgatcctgcc
     4621 gtccaccacg atcggactgt tccagtggcc cgcgccgcag gcgagcgtgg tgatgcgcgt
     4681 gcctgaggcc gcctcgtaca cgttcagtcc gccgttgggt gcatagacgt atagcaggcc
     4741 gtccgcttcg aacgggctgg tgccgtcggt gccgttgctc cacatccggc gcagcttgcc
     4801 gccctgcaat tgccacgctt cggtgccgcg ctcgtcgccg acgaacatcc aggtctgccc
     4861 cgcgtgtttc cataccgccg gctgggtaaa gaggctggcg ccatcggggg tggcgacggt
     4921 ttgcagttcg ccgcccctgt gcggcgtggt cccggcaatg gcttccaggc tgagcagcac
     4981 gatcttgctg tccttgccgc cctgcgcgat caggccgttg ccaagcagca ccggcgaggt
     5041 cgagcccaag tccaggtcgt cctcgttcag atgaccggtg ttggcgggcg tgtagttgcc
     5101 cagcatccgg gtggcatcgg cgtcgagttc gaccagcgaa tcgccccaat tggtcttgcc
     5161 gtcccagtcg gcgttgccgg tcgcgacgaa gatatggccg ctgtcgggat cgatcaccgc
     5221 gccggcgcgt ccccagatcg cggaatcgct ttgcgggcac gaactcggtt ccatcaacac
     5281 ggtgcgatcg ctgcacagcg aattccacac gtggaggagt tggccggatt tgccgtcgag
     5341 gatcgcgacg tggccctggt agggtggcgc gtcgccgatg taccccgccg tcacggcaat
     5401 cacgtggccg cggaagaact tcagcggcga atccatcttt tcgcggtgcg ccaacctggt
     5461 gatggcggtg cgccagacgg catggccgtc gccgatcgcg agcttctgga tatagccgtc
     5521 gggcgacgcg gcatagatga attgccggtc ggggtcggct accggggtgg agttggtgat
     5581 ctggcgggtg cccgccagct tcgaatagct ggccggcgtg taggtccaca ggatcgcgcc
     5641 gctcgtcgcg tcggtcgcca gcgtcttgcc gtaggtggtg gtgacgaaga tcgcgtcgtg
     5701 gcgggcgccg tggatggcga cgccgtgcag gtagatggca ctggcgtcca cggtcccggg
     5761 cagctcgacc tgccggcgcg tgagcttgcc gacgttggcc gcggtgattc cggtgggctg
     5821 ggggttggcg ctggtgctct ggacgttgcc gccgaacatc ggccaatcct gcgcggccgg
     5881 tgtctgggcg aagccggcga cagccacgcc cacgccaagc agcaagccca tcgcgacccg
     5941 gcattccatc ccggaagtat gtcacctgcc ggtgtaacga agcggtgaag aggcgcggca
     6001 gtgccgccgt acccgccact ggcgttcttg ccgaaaggta cttatagttg tacccatgga
     6061 cgaactcacc gaccgccagc gcgagatcct cgacttcatc gcccgccgga tcggtgaggg
     6121 gctgccgccg acgctggtgg agatcgccgg ggcgttcggc ttcaaccagc cgcgggccgc
     6181 gtggaagcac ttgcaggcgc tgcagaacaa gggctgcatc gagcttctgc ccggccgcgt
     6241 acgaggtatc cggctggtgg cggatgcgcc ccgtgccaag cccgccggcc tgccgctggt
     6301 cggccgggtc gccgccggac gcccgatcct cgcccaggag catatcgaac gccatgtcgc
     6361 agtcgatgcc cgcctgttct cgccgcgcgc gcatttcctg ctacgggtgg agggcgattc
     6421 gatgcaaggc gtcggcatcc tcgatggcga cctggtcgcg gtgcaccgca cccccgaagc
     6481 gcgcgacgga cagatcgtgg tggcgcggat cgaggacgag gtcaccgtca agcgcctgcg
     6541 catccgtcgc cagcgggtgc aactgcatgc tgagaatccg gcctatccgc cgatcgaggt
     6601 cgatccgcgt cgcacgacct tcgcgatcga ggggctgtac gtcggcgtaa tccgggtgga
     6661 ttgagatgtc cgaagctgcc ctgcagccgt tgttcgaagg aggccggctc tggcgcgggc
     6721 agggcaacgc cttgccgcgt tcggcgctgc cgaccggatt tgcggcgctg gatgccgtac
     6781 tgcccggcgg cggttggccg gcagcggccc tgagcgaggt cctgctgccc gccgacggcg
     6841 tcggggaatt gcagttgctg tggccgctgc tggcgcggct gtcggagcaa ggcggccgga
     6901 tcgtgttggt cgcgccgcct tacattccgt tcgcgccggc ctgggccgcg gcgggcatcg
     6961 cgctgccgca gttgcaaatc gtgcaaacgg ccgacgaccg caacgcgatg tgggtcgccg
     7021 aacaatgcct gcgttcggcc gcgtgcgcgg cggtggtgtg ctggccgcgg cagggcgatg
     7081 cccgcatcct gagacgcctg cagctcgccg ctgaaaccgg ccagtgccag ggtttcgcga
     7141 tgcggcccgc cggcgcagcc gcgcaacctt cgatggcggc gcttcggatc ctggtcgaag
     7201 ccgatccccc gcaatggcgg gtactcaagt gccgcggcgg caatccgccg gcgcgcgcgg
     7261 tgccgcttcc gtctcgtcat tccggggctg cccgcgttgg cgggcagagc ccggaatcct
     7321 gccttacgta cattggaggc aaaaattgga ttccgggttc catcgccaat ggcgatggcc
     7381 ccggaatgac gacaatcggg gggatggtgg ggtcagaggg agacggacgt tttgcggcgt
     7441 ctgcctcgtg agacgccgtg atgcgctggg cctgcatcct ctttccgcaa ctcgcgctcg
     7501 acgacgtcct gcgccgccgc gccgatcgcg atgcgccgct ggtattgatc gatcccgcgc
     7561 agtcgcgtcc gctggtgcgt gcggtgaacc gccgggcggg tgcggcgggc gtcaaagtgg
     7621 gccagtcgct ggccgtggcg cgcgcgttgt gcgatggact cgtcgtcgcc gatcatgacg
     7681 atgccgcggc ggagcgttgc cgggaattcc tggcggcctg ggcgtacgcc tacagctccg
     7741 aggtcagccg cgatttcccg gatgcgttgt tgctggaagt cgaggcgagc tttcgcctgt
     7801 tcggcggctg gcccaacctg caggcacgct tgcggctgga gctggacgaa ctgggattcc
     7861 ggcaccgcat cgcgctcgca cccaacccgc atgccgcgcg cgtgctggcc ggcgtgcacg
     7921 atggaatcgc gatccccgat gcgatcctgc gcaatgccct gggtgccttg ccggtggagc
     7981 aggcgggcct ggaacccgaa tgcgcgatgg gcctgcgccg gatgggcttg cgcaaactgc
     8041 gtcagatatt cgccttgccg cgcccggaac tcggccggcg gatcggcaag gatgcactgg
     8101 cgcacctcga tcgtctcacc ggcgccctcg ccgcgcccct cactttttac cggccgccgg
     8161 atgcgttcga cgcgtgcatc gaactgccgt tcaacaccgc atccagcgaa tcgctgctgt
     8221 ttccgctgcg gcgcctggtc cgggatttcg cgtcgttcct cgccggccgc gacggcggcg
     8281 tgcagtgctt cacgctcacg ttcgaacacg agcacgacgc ccgcacccgg attcccatcg
     8341 gtctgctctc gccggaacgc gatccgtccc tgctgttcga catcgcacgg gaacgtctcg
     8401 atcgcaccag cctatgtgcg ccggtatgtg cgctgcgtct gcaggcggat gaactgccac
     8461 cgttcgcgcc ctcgcggcgc gacctgttcg acccgcgcac gcagcaggcg gaaccgtggg
     8521 aggcgctgcg cgaacgcctg cgttcgcggc tcggcgacga agcggtgcac ggaatcgccc
     8581 tgcacgcgga ccatcgtccc gaacgtgcat ggcaggtcga acacggcaaa ccacgtgtgg
     8641 acgaaccgcc gcggcatccg cgccccgcct ggctgttgca gcggccgatc cccctgcgtc
     8701 ccgcggtgcg ccagatcctc gccggtcccg aacgcatcga aagcggctgg tgggacggcc
     8761 aggacgtgcg ccgcgattac tacgtcgtcg aaaccggcgc cggccagcgc gcctgggtgt
     8821 tccgtcccgc cggcgagacc ggcgcgttca tgctgcatgg gtggtttgcg tgagaggccg
     8881 gggcttggga cccgagacct tcaagccctc cccctcaggg ggagggttgg gtgggggtgg
     8941 gttgacgaag ccctacgacg caaagaacca acccatcccc accccagccc tccccgtgaa
     9001 ggggagggag aacagcggag tcggggcgga attcggaaca gcagcagcaa gcgcaatcca
     9061 cactatggac cggcgaatcc ttccacccta cgccgaactg cactgccttt cggctttcag
     9121 tttccagcgc ggcgcatcgg tcgctcggga attgttcgag cgcgccaaac gcctgggtta
     9181 ccgtgcgctc gccatcaccg acgaatgctc gctggccggg atcgtgcgcg cgctggaagc
     9241 ttcgcgcaaa accggcgtgc gcctgatcgt cggcagtgaa atgcaagtcc aggacgggcc
     9301 gaagctggtg ctgctggcgc agacgcaagc gggatattcg agattgtgca gcctcatcac
     9361 gttggcgcgc cggcgtgccg acaagggcga gtaccgttcg ttgcgcgaag acttcgccga
     9421 aggctgcaac gacgatttgc tcgcgttgtg ggtgccgggg gccgacatcg acgaaacgca
     9481 tggcgcctgg gtgcgtgacc tttttccgga gcgtgcctgg ctagcggtgg aactccatcg
     9541 cggtcccgac gatgcgacgc ggctcgcgca actgcgcgat gccggcgcgc gcctcggcct
     9601 gccgctggtc gccgcgggcg acgcgcacat gcacgtgcgc agccgccgcg cgctgcagga
     9661 cgtgctgacc gcggtccgcc acaggaccac catcgccgaa gcggggcacc gcctgtatcc
     9721 gaatggcgaa cggcacctgc gcacgcccga ggcgctcgcc gcgatttatc cgcacgcgtt
     9781 gctcgaggaa accttgcgca tcgcggacct ctgcacgttc cgcctcgagg agctccgcta
     9841 cgaatacccg cacgaactgg tgccggcggg acacacgccc acgagctggt tgcgcgaact
     9901 caccgaagcc ggcgcacgca ggcgctggaa gggcggcgtg ccggacaagg tgcgcgcgct
     9961 gatcgaacac gaactcgcgt tgatcgccga gcttcgctac gaatcgtatt tcctcaccgt
    10021 gcacgacatc gtgcagttcg cgcgctcgag cgggatcctg tgccaggggc gcggctcggc
    10081 cgcgaattcc gcggtgtgct acgcgctcgc cgtcaccgag atcgacccgg cgcggatgaa
    10141 catgttgttc gagcggttcc tgtcgaagga acgcaacgaa ccgcccgaca tcgacatcga
    10201 tttcgaacac gaacgccgcg aggaagtcat ccagtacgtg ttcgatcgct acggccgcga
    10261 tcgcgccgcg ctgaccgcgg tcgcgagcag ttaccgcggc cgcggcgcga tccgcgacgt
    10321 cgccaaggcg ctgggcctgc cggcggacct ggtcgaccgg ctggcgcgtt gcatggatca
    10381 ctggggcggt gcggagtcgc tgggtgcgac cctgcgcgag cgcggcttcg atccggactc
    10441 aggcacgctg cgcaaggtgg tggcgctcac cgtgcagctc atcggtatgc cgcggcacct
    10501 gtcgcagcat cccggtggtt tcgtgatgtc cgagcatccg ctgcacacgc tggtgccggt
    10561 cgagaacgcg gcgatggaag accgcaccgt gatccagtgg gacaaggacg acctggacgt
    10621 cctcggcctg ctgaaggtcg attgcctcgc gctcggaatg ctgacggcag tgcgcaagac
    10681 gctcgagctg ttgtgcgcat ccggccgccg cgacatgaag cggctggcgg acattccgtc
    10741 gaaggatccg gacgtgtacg cgatgatcgg ccgcgccgac accatcggcg tgttccagat
    10801 cgaatcgcgc gcgcaaatgg cgatgctgcc gcgcctcagg ccgaagtgct tctatgacct
    10861 cgtgatcgag gtggcgatcg tgcgccccgg tccgatcgtg ggcgacatgg tccacccgta
    10921 cctgcgccgc cgcaatggcg aagagaaggt cacctatccc tccgatgcgt tgcaggcggt
    10981 attggagcgc accctcggcg tgccgctgtt ccaggaacag gtgatgcaga tcgcgatcgc
    11041 ggcggcgggc tattcgccgg gcgaagccga cgagttgcgc cgttcgatgg cggcgtggaa
    11101 acgccacggc gggctcgagc cgcatcgtga ccggctcgcc gccggcatgc gcgcgcgcgg
    11161 ctacagccaa gcctatgccg accatttgtt cgagcagatc aaggggttcg gcagctacgg
    11221 atttcccgaa tcgcacgccg cgagtttcgc gctgatcacc tgggtcagct gctggctgaa
    11281 attccacgaa cccgcggcgt tcgcctgcgg ggtcatcaac tcgcagccga tggggttcta
    11341 ttcgccggac cagttgttgc aggacgcacg ccgccatggc atccgggtgc tgccggtcga
    11401 tgtccgctac agcggctggg attgctcgct ggaggaccgc agcccgggct cggcacaacc
    11461 ggcgatccgg atggggttcc ggatgatccg cggtttccgc gaagatgccg ccgccgcgat
    11521 cgtggcggcg cgcgccgcgc gcgcgttcac cgatgtcgcc gacctgtgcg cgcgtgcatc
    11581 attggacgtc cgccagcgca gcttgctcgc cgacgcggcg gcattgcgcg ggctggccgg
    11641 ccaccgccac cgggcgcgct ggacggtcgc cggcgtggag acctcaccgc cgctgttcat
    11701 cgatgcaccg gccacgaagg aaaccgcgat cacgctgccg ctgccgacgc gcgccgagga
    11761 agtccgcacc gattacgcga tgaccggcac cacgctcggt gcgcatccgc tgtctttcct
    11821 gcgcgcgaca ttgtcgcggc gccgttgtcg gcgctccgcg gaattgaaac gttgtccgaa
    11881 cgggcagcag gtgcgcacgg cgggcctggt caccctgcgg cagcgccccc agaccgcgag
    11941 cggcgtgatc ttcatgaccc tggaagacga ggacggcatg accaacgtcg tcgtgtggaa
    12001 cgacctcgcg caacgccagc gtcgcgaact gctggaatcg cgcctgctcg cggtggaggg
    12061 tcggctggag aaccgccacg gcgtgcagca cctgatcgcc gaacgcctgc acgactggac
    12121 gcggttgttg ggcgcgctgg atacgcgctc gcgcgatttc cactgacgat atttcccctc
    12181 tcccctcggg agagggtgcc ccgtcagggg cgggtg
//
LOCUS       DASSNH010000090         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1679298, whole genome shotgun sequence.
ACCESSION   DASSNH010000090 DASSNH010000000
VERSION     DASSNH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679298"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFK00_03120"
     CDS             <1..814
                     /locus_tag="VFK00_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591976.1"
                     /translation="PDQQQRDLALAERQLLSDDWHGLADRIETALKQPGCSAPNWMPV
                     FASAFGYGDLIENLGARVNVCDPLNGINYRTRVSVALATGHPQRALDVLAMYAKASGD
                     GPMPTAPQAIALAMLGRIGEAQATLASMEPRGDGYYIAQLSVGKAAGEDPVKLRASLQ
                     DVDRKNSKLQMEGITDAVAAALSGNRAQANRRAGALDARPAGGLLLAIVVTECQCGAP
                     FDLDATPRFKTLLAESGLRWPPPQTIKYPARAPANGAKLIPAPTTAKDTAHD"
     gene            807..932
                     /locus_tag="VFK00_03125"
     CDS             807..932
                     /locus_tag="VFK00_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591977.1"
                     /translation="MTDLRQRTKERKLVQWTVAYAAGAFALLQGVDMVAATATSG"
     gene            1398..2633
                     /locus_tag="VFK00_03130"
     CDS             1398..2633
                     /locus_tag="VFK00_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7591978.1"
                     /translation="MNAIAVVSPAPVEPLPGERASGSPEQGIAICLSGGGYRAMLFHT
                     GVIWRLAELGLLGPATHNFRKADGSLQPAGTLQRISSVSGGSLLAGMLGLKWKDLAVA
                     EPGLVDRYVELVVAPIRKLAGVSLAGKDTQGVMHMLWNIVSPGSVNDHIIAAYKKHLF
                     GDATLQDLPDSPRFVINASNLQSGALWRFMKPYMRDWRVGEVKRPTISLAQAVAASSA
                     FPPVLAPAPLSLEDTDYTPNSGGKGRDDLQRAPFTTRPVLADGGVYDNLGLETAYKRY
                     QTLFVSNAGAPFGIQPEVHSDWVRLGQRCIDVMDNQVLSLRKRLLVGDLVAGVRNGAF
                     WDIEQDIAVHGCAQALVCPGDATTRLAQVGTDLSTKDAAIQERLINWGYAVSDAAVRG
                     HFDGGLPAPRGFPYPGGVG"
     gene            2687..4456
                     /locus_tag="VFK00_03135"
     CDS             2687..4456
                     /locus_tag="VFK00_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591979.1"
                     /translation="MARRAVHGNRPDVQTEPAPTRGESVPLRSLTILAQDPAVRRDGK
                     ILLATAQITAEPLMDGPCGARIKVVDYDASRDRLYLPMKGGYQQGEECLDPFAGKNPA
                     RFLDDPRFHQQNVYAIAMRTLGVFESALGRRTCFGFQGHQLHIAPHAFAGPNAYYQRE
                     NRCLCFGYFPGPDGQFVHTCLSHDVVAHEMTHALLDGLRPRFIDPSSPDQAGFHEGFA
                     DVVALLSVLSDRAIVEQVLIRRKVLRDRRALIATSELTASALKASTLLGLAEQVGTSI
                     PNGAATALRRSVELKASPRWLQDPDCEEPHRRGEILGAAVMDAFLAVWVARVASLGTV
                     SRGQCDGDRVVEEGVAAANHLLTMCIRALDYAPSTDLQYVDYLSALLTADKETVPDDS
                     KYHYRQIIRNSFRAWGIEPVSHPDGDGCWESFDRRVSYRNLRSESLRRDSDEAFRFIW
                     QNFDALQLAREAYTEVQAVHPVARVAPDGLIVRETVIDYLQILTVRGHELRALGLRRP
                     EELPTSKEVRLYGGGVLVLDEFGRLKYSIGNNVANVARQQPRLDYLAKMGFFEYRSDQ
                     DFSRGFFARLHLARARDRWTPGG"
     gene            4459..>5049
                     /locus_tag="VFK00_03140"
     CDS             4459..>5049
                     /locus_tag="VFK00_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7591980.1"
                     /translation="MAPASVRVRMYHVGFGDCFLLTFRYPGRTKPRHVLIDFGTTAAP
                     SGSPAKLLARIAEDIRKTVGRDPFAVVATHRHRDHIAGFDPGSNGKGPGAVIAALKPQ
                     FVVQPWTEQPDLAVDAKAPPGFKGLAARRETLASLQAIARHAVEEALPKLQRSFRHTA
                     TAEQLAFIGEDNISNEGAVRNLMGMAPNDYVYAGKTT"
BASE COUNT          861 a         1639 c         1666 g          883 t
ORIGIN      
        1 cccggatcag cagcagcgcg atctggcgct ggccgagcgg caattgctga gcgacgactg
       61 gcatggtctg gccgatcgca ttgaaaccgc actcaagcag cctgggtgca gcgcaccgaa
      121 ctggatgccg gtattcgcca gtgcgttcgg ctatggcgat ctgatcgaga acctcggtgc
      181 gcgcgtcaat gtctgcgacc cgctgaacgg tatcaactat cgtacccggg tgtcagtggc
      241 actggcgacc ggacatccgc agcgcgcgct ggatgtactg gcgatgtacg cgaaggcaag
      301 tggcgatggt cccatgccaa cggcaccaca ggccatcgca ctggccatgc tcgggcggat
      361 cggggaagcg caggccacgc tggcatcgat ggaaccgcgc ggcgatggtt actacatcgc
      421 acaactgagc gtgggtaagg ccgccgggga agatccggtg aagctgcgtg ccagcctgca
      481 ggacgtcgat cgcaaaaact cgaagcttca aatggagggc atcaccgacg cggtggcagc
      541 cgcgttgtcc ggtaaccgcg cccaagccaa tcgacgcgct ggggcgctag acgcgcgacc
      601 ggcgggcggc ctgctgcttg ccatcgtggt caccgaatgc cagtgcggcg cgccattcga
      661 cctcgacgcc acaccgcgtt tcaaaacgct gctcgccgaa tccggactgc gttggccgcc
      721 accacaaacc atcaaatacc ccgcccgcgc tccggcgaac ggagccaagc tcattcctgc
      781 gccaacaaca gcaaaggaca ccgcgcatga ctgatcttcg gcaacgcacg aaggaacgca
      841 agctggtgca gtggacagtg gcttatgcgg cgggcgcgtt cgcgttgctg caaggcgtgg
      901 acatggtggc cgccaccgcg acgtctggat gaaatctacc taggagccaa agttaacgca
      961 ccacatccga tagtcgcact cgtcatcgcg ttgctggttc ttggtgcagt acttttgatg
     1021 ctcgtgccgc ttgcgcgatg gtgcgttcgc tgtgcgcgtc cagatcgagt tcaatcagag
     1081 tcagcctgac ttgcgatctg tgtacacagc ggcgaccgag tattggggag tagtcgtatg
     1141 cggatctagc gcttctacgc cgagtcaggg accaaaggcc gctatcgggc gacttggtaa
     1201 agccggcagc cgtccgcttt tggcccgatg gccgccatcc gaaccagtga tgaccggcgt
     1261 ctgcgagcgc ctacgggcac ccgcggcagc aggtctcgtg attcctgagg ctccgctaca
     1321 gcgcgccttc gaaaagttgg cgcgcacgcc gttccaaatg gctatgatct gcctcgtgac
     1381 gggcgagggg attggccatg aatgcgatcg ccgttgtttc accggctccg gtcgagccgc
     1441 ttccgggcga gcgggcttct ggatcacccg agcaggggat cgccatatgc ctttccggcg
     1501 gtggctaccg cgccatgctg ttccacacgg gcgtcatctg gcggctggcg gaactcggcc
     1561 tgctgggccc tgccacgcac aacttccgga aggccgacgg ttcgctgcag ccggccggca
     1621 cgctgcaacg gatctccagc gtctcgggcg gttcgttgct cgcgggcatg ctcgggctga
     1681 aatggaagga tctcgcggtg gcggagcccg ggctggtcga ccgctacgtc gaattggtgg
     1741 ttgcgccgat ccgcaagctt gccggcgtct cgcttgccgg caaggacacc cagggcgtaa
     1801 tgcacatgct ctggaacatc gtttcgccgg gttcggtgaa cgaccacatc atcgcggcct
     1861 acaagaagca cctgttcggt gacgcgacgc ttcaggattt gcctgattcg ccgcggttcg
     1921 tcatcaacgc ttcgaacctc cagtcgggtg ccttgtggcg cttcatgaaa ccgtacatgc
     1981 gcgattggcg cgtgggcgag gtgaagcggc cgaccatttc gctggcgcaa gccgtcgccg
     2041 catcgtccgc gtttccaccc gtcctcgcgc ccgcaccgct gtcactcgag gacacggact
     2101 acacgccgaa ctcgggcgga aagggccgcg acgatttgca gcgcgcaccg ttcaccacca
     2161 ggccggtgct ggccgacggc ggcgtgtacg acaacctggg cctggaaacg gcctacaagc
     2221 gctaccagac gctgttcgtc agcaatgccg gtgcgccttt cggcatccaa cccgaggtcc
     2281 actcggattg ggtccgcctg gggcagcgct gcatcgatgt gatggacaat caggtactca
     2341 gcttgcgcaa gcgtttgctg gtcggcgacc tcgtggcggg cgtgcgcaac ggtgcgttct
     2401 gggacatcga acaggatatt gccgtgcacg ggtgtgcgca ggcgctggtg tgtccgggcg
     2461 atgccaccac gcgcctggcg caagtcggca cggacctttc gaccaaggac gctgccatcc
     2521 aggagcgctt gatcaattgg ggctatgccg tctcggatgc ggcggttcgc gggcacttcg
     2581 acggcgggct gccggcgccg cgcggatttc cgtaccccgg cggcgtgggg tagattcgaa
     2641 atcgcaagag cgggatcgtg attcgacacc tgtagtggag acgtccatgg ctcgccgcgc
     2701 tgtccatggc aatcgaccgg atgtgcagac ggagccggcg cccacgcgag gcgagtcggt
     2761 gccgctgcgc tcgttgacga tcctggcgca ggatccggcg gtccggcgcg acggcaagat
     2821 cctcttggcc acggcgcaga tcacggccga accgctgatg gacgggcctt gcggcgcacg
     2881 catcaaggtg gtcgattacg acgcctcgcg cgatcgcctt tatctgccca tgaaaggtgg
     2941 ttaccagcag ggcgaggagt gcctcgatcc ctttgccggc aagaacccgg cacggttcct
     3001 cgacgatccc cgcttccacc agcagaacgt ctacgcgatc gcgatgcgca ccctcggtgt
     3061 gttcgaaagc gcgttgggcc ggcgcacctg cttcggcttc cagggccacc agctccacat
     3121 cgcgccgcat gcttttgccg gtcccaacgc ctactaccag cgcgagaacc gttgcctgtg
     3181 cttcgggtac ttcccggggc cggatgggca gttcgtgcat acctgtttgt cccacgacgt
     3241 cgtcgcgcac gaaatgacgc acgcgctgct cgatggcctg cgtccgcgtt tcatcgatcc
     3301 ctcgtcgccc gaccaggccg ggttccacga aggattcgcc gacgtggtcg cgctgctgtc
     3361 ggtgctgtcg gaccgcgcca tcgtcgagca ggtcctcatc cggcgaaagg tgttgcgcga
     3421 ccggcgtgcc ctgatcgcga ccagcgagtt gaccgcatcc gcgctcaagg cttcgacgct
     3481 gctggggctg gcggaacagg tgggcacgtc cattcccaac ggcgcggcca cggcgctgcg
     3541 gcgctcggtg gaactgaagg cctcgccgcg ctggctccag gatccggact gcgaagaacc
     3601 gcaccgtcgc ggcgaaatcc tgggcgccgc ggtgatggac gcgttcctcg cagtgtgggt
     3661 ggcccgcgtc gcgagcctgg gtaccgtctc gcgcgggcag tgcgatggcg atcgcgtcgt
     3721 cgaagagggc gtcgcggcgg ccaaccacct gctgacgatg tgcattcggg cgctcgacta
     3781 cgcgccctcc acggatctcc agtacgtcga ttacctgagc gcgctcctca ccgccgacaa
     3841 ggaaacggtc ccggacgatt cgaagtacca ctatcggcag atcatccgga actccttccg
     3901 cgcgtggggc atcgaaccgg tgagccaccc cgacggtgac ggatgctggg aatccttcga
     3961 ccggcgggtg tcgtatcgca acctccgttc cgaatcgttg cgccgcgatt ccgacgaggc
     4021 gttccggttc atctggcaga acttcgacgc gctgcagctg gcgcgcgagg cgtataccga
     4081 agtgcaggcg gtgcatccgg tggcgcgggt ggcgcccgac gggctgatcg tccgcgagac
     4141 cgtgatcgat tacctgcaga tcctcacggt gcgcgggcat gagttgcgtg ccctcggact
     4201 gcggcgtccc gaggaacttc ccaccagcaa ggaggtgcgg ctgtacggcg gcggtgtgct
     4261 ggtgctcgat gaattcgggc ggctgaaata cagcatcggc aacaacgtgg cgaacgtcgc
     4321 ccgccagcag ccgcgcctgg attacctggc caagatgggc ttcttcgagt accgcagcga
     4381 ccaggatttc agccgcggtt tcttcgcacg cctgcacctg gcgcgtgccc gcgaccgctg
     4441 gaccccggga ggttgagcat ggctccggcg agcgtgcggg tgcgcatgta ccacgtcggc
     4501 ttcggcgact gcttcctgtt gacgttccgc tatccggggc gaacgaagcc gcgccacgtg
     4561 ctgatcgact tcggcaccac agctgcgccg agcggcagcc cggcgaaact gctggccagg
     4621 atcgcggagg acatccgcaa gacggtgggc agggatccct tcgccgtggt cgcgacccac
     4681 cgccaccgcg accacatcgc cggcttcgat cccggttcga acggcaaggg accgggcgcc
     4741 gtcatcgccg cgctcaagcc ccaattcgtg gtgcaaccct ggacggagca gccggatctc
     4801 gccgtggatg ccaaggcgcc tccgggattc aagggattgg cggcccgtcg ggaaaccctg
     4861 gcatcgctcc aggccatcgc ccggcatgcc gtggaagagg cgcttcctaa gctgcaacgc
     4921 tcgttccggc acaccgcgac cgccgagcag ttggcgttca tcggcgaaga caacatttcc
     4981 aacgagggcg ccgtccgcaa cctgatgggg atggcgccca acgattacgt gtacgccggg
     5041 aagaccacc
//
LOCUS       DASSNH010000091        12051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1693984, whole genome shotgun sequence.
ACCESSION   DASSNH010000091 DASSNH010000000
VERSION     DASSNH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12051
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693984"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..666
                     /gene="mraZ"
                     /locus_tag="VFK00_03145"
     CDS             220..666
                     /gene="mraZ"
                     /locus_tag="VFK00_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET7591981.1"
                     /translation="MFQGETAISVDEKGRLAIPTAYREPIARVCNSRLVITYNPFEPG
                     CLWVFPQPEWERVRDQVNALSSVKRVHRDLQLKLVGAAAQVELDGAARVLLPASQRAA
                     SGIEKKAVLLGMGNKFELWSEQAHLAKIHQTIGEEEVSEEMVGLRL"
     gene            765..1706
                     /gene="rsmH"
                     /locus_tag="VFK00_03150"
     CDS             765..1706
                     /gene="rsmH"
                     /locus_tag="VFK00_03150"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7591982.1"
                     /translation="MTGSHGLMHIPVMRDAVLAGLALKPDGRYLDGTFGRGGHARAIL
                     ERLGADGRLLLMDRDPEAIACATREFGADPRVAIRHANFSEMDDWEDTRAGLDGILLD
                     LGVSSPQLDDAGRGFSFQSDAPLDMRMDPTTGESAAAFLARAGEREIADVLFRFGEER
                     MSRRIARAIVARRDEAPLATTRELAELVERALGRRDRKHPATRTFQALRLQVNDELGS
                     LERALPAAAATLKPGGRLAVISFHSLEDRIVKQFIRGPQPRPVRRGLPPPPMATRPPL
                     RAVGRKLVADPAEVARNPRARSAVLRISEKPPSDGAA"
     gene            1703..1975
                     /gene="ftsL"
                     /locus_tag="VFK00_03155"
     CDS             1703..1975
                     /gene="ftsL"
                     /locus_tag="VFK00_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET7591983.1"
                     /translation="MRTVGALAVGVLLLAVIASGIAVVWARHQDRAAFVELSKLQNQR
                     DALNVEFGRLELEQATWASPSRIETIARGQLGMISPPPASVEMIHE"
     gene            1972..3774
                     /locus_tag="VFK00_03160"
     CDS             1972..3774
                     /locus_tag="VFK00_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7591984.1"
                     /translation="MKPRRPPETISRPQRRRRPDPRKRLALVVGVLALASIGLIARAV
                     DLQVVHQAFLQQQGDARFLREVEIPVSRGTIYDRNGAPLAVSTPMLSLWANPEQVLQH
                     SERIPELAKALGVDASDLARKFQERSDREFVYLRRLMPPEAAQAVLALDIPGVNGQRE
                     YKRYYPDGAATAHVLGFTNIDDRGQEGLELAYDGWLAGKPGEKRVIRDRLGRVVENVE
                     QIRAPVPGHDLALSIDSRIQYLAYSALSEAVLKRHAGSGSMVIMDPRNGEILAMVNVP
                     SYNPNDLDSSTAAQRRNRAVTDVTEPGSTMKPFTVTTALESGKWTPTTMLDTGPGWFM
                     IDGHTVRDDSNNGRIDVSHVITYSSNVGASKISLTLTPQEMSGVLHQFGFGASTGSGF
                     PGEVSGYIPAPRTWGDFVKATIAFGYGLNVTALQLAQAYSAIADGGVRHTPTFIKGDD
                     NPGVRVEPLKISDEVMAMLRTVVSPEGTAPQAAIADYSVAGKTGTAHLAIAGGYSRNR
                     YAALFCGIVPASNPRLVGVVIIRDAGGHGGEYFGGIVSAPVFREVMTGALRLLDVPPD
                     NVQQWYAGGPVTPAPPPAPPEAVATARRGQGTTP"
     gene            3771..5282
                     /locus_tag="VFK00_03165"
     CDS             3771..5282
                     /locus_tag="VFK00_03165"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7591985.1"
                     /translation="MSAMTLAELLDGIASAADGGIVVSGLTQDSHAVRAGDAFVALQG
                     ARTHGMAFAPDAVARGAVAILAEVGTGNREQGTAASAPALQPRSPIPVPAVWIEGLRT
                     HLGAIASRFHGDPSASLHVVGVTGTNGKTSTVQLLAQAFTLLGHRAATIGTLGAGLHG
                     ALHEGERTTPDVIRVHGLLAEFRDAGATHVAMEVSSHALDQHRVDGVRFDAAVFTNLT
                     RDHLDYHHTMEAYGAAKARLFAWPGLRAAVINVDDAFGRELAARLPTRVHTLRCATGA
                     GDAELRARDVHTHSDGIDFELVTPWGSGAVRSRLLGRFNVANLLAVAGVLGALDVPFA
                     QMLPALESLQPVAGRMSRIGGGALPLVVVDYSHTPDALEQALLTLRAHCEGKLICVFG
                     CGGERDAGKRPEMGGIAERLADLAIVTDDNPRGEDGDAIVAQILAGMGSPERAIVERD
                     RAHAIDLALARASEGDVVLLAGKGHEAYQESAGVKRAFDDLQVARAALEARTC"
     gene            5279..6646
                     /gene="murF"
                     /locus_tag="VFK00_03170"
     CDS             5279..6646
                     /gene="murF"
                     /locus_tag="VFK00_03170"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7591986.1"
                     /translation="MNLRLSEIAVWTRGSLRGADASVAAISTDSRTLAAGDLFVALVG
                     ERHDGHDHVAEAAARGAAAALVSRPVDTDLPQVVVADTLHALGDLASAVRARRDVTVV
                     GITGSNGKTSVKTLTAQILARHGHTHSNAASFNNEIGVPLTLLAMPDDTEYAVLEMGA
                     GKPGDIDYLAAIVRPRIGLVNNIGPAHLERMHSLEGIAETKGAIYRALPPDGVAVINA
                     DDAFAAYFAGLAGARRVLRVGLEKPADITAEDLKLGPDASGFTLVTPVGRAAVRLPLP
                     GHHNIANALAATALAQAAGAALDDIVAGLQSAESIGGRLLRHASPEGWVLFDDSYNAN
                     PGSALAAVATLALQPGERWLVLGDMAELGAGAGDLHAKIGAAAREQGIARVYAVGALA
                     ARAAEAFGQGGSVHPDQSALVAALRGDLHAGASVLVKGSHSSHMERVVAALLANDEGG
                     DRHVA"
     gene            6636..7718
                     /gene="mraY"
                     /locus_tag="VFK00_03175"
     CDS             6636..7718
                     /gene="mraY"
                     /locus_tag="VFK00_03175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7591987.1"
                     /translation="MLLELAHWLEQYFGPFRLFDYITFRTIMAALTGLFFSLFFGPAM
                     IRRLTALKAGQVVRTDGPQTHLAKAGTPTMGGALILVAIVVGTILWGDLHSRYLWVVL
                     VVTLACGLIGFYDDYRKLVLKDPHGLIARWKYLAQSIVGLGAALFLFHTADVPAATAL
                     YVPLFKHVVIPLGAGFVVMAYLWIVGFSNAVNLTDGLDGLAIMPAVLVTAALGAFAYL
                     AGNAVFSAYLGIPSIPGAGELAVFCGALTGAGLGFLWFNTYPAQVFMGDVGALAIGGA
                     IGCVAVIVRQEIILLVMGGLFVVETASVMLQVGSFKLTGRRMFRMAPIHHHFELKGWP
                     EPRVIVRFWIISVVLVLVGLATLKVR"
     gene            7834..9117
                     /gene="ftsW"
                     /locus_tag="VFK00_03180"
     CDS             7834..9117
                     /gene="ftsW"
                     /locus_tag="VFK00_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET7591988.1"
                     /translation="MSLFAPSQATRRGGPRGRHDPWLLLAIVGLAAFGIVMVASSSIA
                     VADGHHIGEFYYLQRHVTFLALGGAIALWIALRIELNTLERWSGWLMVFTFALLVLVF
                     IPHIGMRINGARRWINLGISGFQPVELAKLTVVLYVASYLVRHRDSVETKFLGAVKPV
                     AVAGFTAVLLLAQPDFGSAALIGVVTIGMLWLGGARMRNLVIMGLPLVPLGSWLALST
                     DYRVKRLTSFLDPWKDPFNDGFQLTQALIAVGRGKWFGVGLGGSVQKLFYLPEAHTDF
                     ILAVIAEELGFVGVLFVLALFALLVGRGLQLGLRGVEVGQHRAGYVASGISLMIGLQA
                     LVSVGVNLGVLPTKGLTLPLISSGGSSTLMTCIMLGVLVRAGYEIHRAEDARRMATRR
                     APGPITDDIPIEKPAAELPLAARKRIEPRLDEVRA"
     gene            9114..10199
                     /gene="murG"
                     /locus_tag="VFK00_03185"
     CDS             9114..10199
                     /gene="murG"
                     /locus_tag="VFK00_03185"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET7591989.1"
                     /translation="MSDARAPVLIMAGGTGGHIFPGLAVAAALGAREVPVVWLGARGG
                     MENRIVPEHRIEMRTVPVAGLRGKGAAVRLAAPWMLLRAILAAWRVLRDIRPRSVLSL
                     GGYAAGPGGIAAWLQRRPLLVHEQNRVPGFTNRILAKFARKVMGGFPDAFAPGVHSEW
                     TGNPVRSEITALPGPAARFADRAGVPRLLVLGGSLGARTLNLGLPEALSLIPRDRRPE
                     VWHQCGEAHLETTRTAYAGVDVHAKVAPFILDMAAAYAWADLVVCRAGALTLAELAAA
                     GLGAILMPFPYAVDDHQTKNAEGFVAAGAAELVQDRDFDPARFAALLERLLGDAAKRL
                     AMAKAARVLAKPDAADVIAQRCLEVAA"
     gene            10196..11635
                     /gene="murC"
                     /locus_tag="VFK00_03190"
     CDS             10196..11635
                     /gene="murC"
                     /locus_tag="VFK00_03190"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7591990.1"
                     /translation="MTPHRLPPHGDIMQGFRHVHFIGIGGAGMSGIAEVLHTLGYDVS
                     GSDRAQSPVTERLVKMGVDVAIGHAAEHIRNVDAVVVSSAIKPDNPELIAARERRIPV
                     VPRAEMLGELMRFRRGIAVAGTHGKTTTTSLVASVLAEAGYDPTFVIGGQLLSAGAHA
                     RLGAGDYLVAEADESDGSFLMLSPVAAIVTNIDADHLEHYHGDYAELKQAFVDFLHRL
                     PFYGLAVLCVDDPDVAELAQRTPRSTLTYAIDRAADVRGANLRAEGPCMRFDLQLPGQ
                     PPLPVTLNLPGRHNVLNALAAATLGWHLGIEPFAIARALERFEGVGRRFHINGELAVD
                     DGRVMLVDDYGHHPREIAAVMEAARAGWPERRVVIVFQPHRYTRTRDLMDDFAAVLCE
                     ADVLVLTDVYPAGEMPIANADGRALARAVRARGKVDPVFIDHPRELRETLPALLHDGD
                     LVLLLGAGDIGAAAAELARGGDLKTDGGV"
     gene            11773..>12051
                     /locus_tag="VFK00_03195"
     CDS             11773..>12051
                     /locus_tag="VFK00_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7591991.1"
                     /translation="MGGSSAEREVSLDSGRGVLEALKSRGVDAHAIDGIPALLDAVRA
                     GHFARVFNILHGQHGGGEDGVLQGALDSLHVPYTGSGVLGSALSMDKVR"
BASE COUNT         1801 a         4046 c         4261 g         1943 t
ORIGIN      
        1 gcatcacggc ggccaatccg ggcaaatcca aaaaacgtcg tgatgacaaa tcttttggat
       61 tcaaaggcct tgccaacgta gctcacaagg cacctgaatt ccagtcacaa cctcgccttt
      121 ccctttgatc tacaagcaaa aattgcttga cagtctcatc ccctgcgact aaggtgggct
      181 cacgtgtcgt tttgtgggaa tacgtgggaa ttctgaccga tgtttcaggg cgaaaccgcg
      241 atcagcgtgg acgaaaaagg gcggctggcg attccgaccg cctaccgcga gccgatcgcg
      301 cgcgtctgca acagccggct ggtgatcacc tacaacccgt tcgagccggg atgcctgtgg
      361 gtgttcccgc agccggagtg ggagcgcgtg cgcgaccagg tcaacgcatt gtccagcgtc
      421 aagcgcgtgc accgcgacct gcaactgaag ctggtgggcg cagccgcgca ggtcgagctc
      481 gatggcgccg cgcgggtact gctcccggcc agccagcgcg ctgcatccgg gatcgagaag
      541 aaggccgtgc tgctggggat gggcaacaag ttcgaactat ggagtgaaca ggctcacctc
      601 gccaagatcc accagacgat cggcgaggag gaagtcagcg aggaaatggt gggattgagg
      661 ttgtgaggat cgcgtccggc gaacggcggg cagcgccggc tcggacgatg cgggtggcac
      721 atgcgcgcaa gcggcgtgcg cgacggatgg acgggacgcg ggcaatgacg ggcagccacg
      781 ggctcatgca catcccggtg atgcgggacg cggtgctggc cggactcgcg ctgaagccgg
      841 atgggcgtta cctcgatggc acgttcgggc gtggcggcca tgcgcgcgcg atcctcgaac
      901 ggctcggcgc ggacgggcgc ctgctgctga tggaccgcga cccggaagcg atcgcctgcg
      961 caacgcgtga attcggcgcc gacccgcggg tggcgatccg gcacgccaat ttctccgaaa
     1021 tggacgactg ggaagacacg cgcgcggggc tcgacggaat cctgctggac ctcggcgtgt
     1081 cttcgccgca gctcgacgac gcggggcgcg ggttctcgtt ccagtccgac gcgccactcg
     1141 acatgcgcat ggatccgacc accggtgaaa gcgcggcggc gttcctcgcg cgggccggcg
     1201 agcgcgagat cgccgacgtg ctgttccgct tcggcgaaga gcgcatgagc cgccgcatcg
     1261 cccgtgcgat cgtcgcccgc cgcgacgagg cgccgctcgc gaccacccgc gagctcgccg
     1321 aactggtcga gcgcgcgctg ggacggcgcg accgcaagca tccggcgacg cgcaccttcc
     1381 aggcgctgcg gctgcaggtc aacgacgagc tgggctcgct cgagcgcgcg ctgccggcgg
     1441 cggcggcgac cctgaaaccc ggcggtcggc tggcggtgat cagtttccat tcgctggagg
     1501 accgcatcgt caagcaattc atccgcggcc cgcagccgag gccggtgcga cgcggtttgc
     1561 cgccgcctcc gatggccacg cgcccgccgc tgcgggcggt cggccgcaag ctcgtcgccg
     1621 atcccgcgga agtcgcgcgc aatccgcgcg cgcggtcggc ggttctcagg atctccgaaa
     1681 aaccgccgtc ggatggtgcc gcatgaggac cgtcggagcc ctcgcggtgg gggtgctgct
     1741 gctggcggtg atcgcgagcg gcatcgcggt ggtgtgggcg cgccaccagg accgcgctgc
     1801 cttcgtcgaa ctttcgaagc tgcagaacca gcgcgacgcc ttgaacgtgg agttcgggcg
     1861 cctcgaactc gagcaggcga cctgggcgag cccctcgcgc atcgagacga ttgcgcgcgg
     1921 gcaactcggc atgatcagtc cgccccccgc gagcgtggag atgatccacg aatgaagccg
     1981 cgccgtccgc ccgaaacgat ttcgcggcca cagcgccgcc gccgccccga tccgcgcaag
     2041 cggctcgcgc tggtggtcgg cgtgctcgcg ctcgcctcga tcggcttgat cgcgcgcgcg
     2101 gtggacctgc aggtggtgca ccaggcgttc ctgcagcagc agggtgacgc gcgcttcctg
     2161 cgcgaggtcg agattcccgt ctcccgcggc acgatctacg accgcaacgg cgctccgttg
     2221 gcggtgtcca cgccgatgct ctcgctgtgg gccaatccgg agcaggtgtt gcagcacagc
     2281 gagcgcatcc ccgaacttgc gaaggcactg ggggtcgacg ccagcgatct cgcgcgcaag
     2341 ttccaggaac gcagcgaccg tgagttcgtc tacctgcggc ggctgatgcc gccggaggcc
     2401 gcgcaggcgg tgctggcgct ggacatcccc ggggtcaacg gccagcgcga atacaagcgc
     2461 tattacccgg acggtgccgc aaccgcgcac gtgctcggct tcaccaacat cgacgaccgc
     2521 ggccaggaag ggctggaact cgcctacgac ggctggctgg ccggcaagcc cggcgagaaa
     2581 cgcgtgatcc gcgaccgcct gggccgcgtg gtcgaaaacg tcgagcagat ccgtgcgccg
     2641 gtgccgggcc acgatctggc cctcagcatc gacagtcgca tccagtacct cgcttactcg
     2701 gcgctttccg aggccgtcct gaagcgccat gcggggtccg gctcgatggt gatcatggat
     2761 ccgcgcaacg gcgagatcct cgcaatggtc aacgtgccct cgtacaaccc caacgacctc
     2821 gacagcagca ccgccgcgca acgccgcaac cgcgcggtca ccgacgtgac cgagccgggc
     2881 tcgacgatga agccgttcac ggtcaccacc gcgctggagt ccggcaagtg gacgccgaca
     2941 accatgctcg atacgggacc gggctggttc atgatcgatg gccacaccgt ccgcgacgac
     3001 agcaacaacg gtcgcatcga cgtcagccac gtcatcacct attcgagcaa tgtcggtgct
     3061 tccaagattt cgttgaccct gacgccgcag gagatgagcg gcgtgctgca tcagttcggg
     3121 ttcggcgcga gcaccggcag tggttttccg ggggaagtgt cgggttacat cccggcgccg
     3181 cgcacatggg gcgatttcgt caaggcaacg atcgcgttcg gctatgggct caacgtgacg
     3241 gcgttgcaac tcgcgcaggc gtattcagcg atcgcggacg gcggcgtccg gcacacgccg
     3301 accttcatca agggcgacga caaccccggc gtcagggtcg aacccctgaa gatttccgac
     3361 gaggtgatgg cgatgttgcg cacggtggtg agcccggagg gcaccgcgcc gcaggccgcg
     3421 atcgccgatt acagcgtcgc cggcaagacc ggcaccgcgc atctggcgat cgcgggcggc
     3481 tactcgcgaa accgttacgc ggcgctgttc tgcgggatcg tgcccgccag caatccgcgc
     3541 ctggtcgggg tggtgatcat ccgggacgcg ggcggccatg gtggcgaata tttcggcggc
     3601 atcgtgtccg cgccggtatt ccgcgaagtg atgaccggcg cgctgcgcct gctcgacgtc
     3661 ccgccggaca acgtgcagca atggtatgca ggcgggcccg tgacgcccgc gccgccgcct
     3721 gcgccacccg aagcggtggc gaccgcgcgc cgcgggcagg ggacgacgcc atgagcgcga
     3781 tgaccctggc cgaactcctg gacggcatcg cgtccgcggc agacggcggc atcgtggttt
     3841 ccgggctcac ccaggactcg cacgccgtgc gcgccggcga cgcgttcgtg gcgctgcaag
     3901 gcgcgcgcac gcacggaatg gcttttgcgc cggatgcggt ggcgcgcggt gccgttgcca
     3961 tacttgcaga agtgggaaca gggaacaggg aacagggaac cgcggcatcc gcgccggctt
     4021 tgcagccccg ttccccgatc cccgttcccg ctgtatggat cgaaggcctg cgcacccacc
     4081 tcggcgcgat cgcgtcgcgt ttccacggcg atccttcggc gtcgctgcac gtggtcggtg
     4141 tcaccggcac caacggcaag acttcgacgg tgcagttgct ggcgcaggcg ttcacgctgc
     4201 tcggccatcg cgccgccacg atcggcaccc tcggcgcggg gctgcacggt gcgctccacg
     4261 agggcgagcg caccacgccg gacgtgatcc gggtgcatgg cctgctcgcc gaattccgcg
     4321 atgccggcgc cacgcacgtc gcgatggaag tttcctcgca cgcgctggac cagcatcgcg
     4381 tcgatggcgt gcgtttcgac gccgcggtgt tcaccaacct cacgcgcgac cacctcgact
     4441 accaccacac gatggaagcc tacggggcgg ccaaggcgag gctgttcgcg tggccgggct
     4501 tgcgcgcagc ggtcatcaac gtggacgacg cgttcggccg cgaactggcc gcaaggctgc
     4561 cgacccgcgt gcacacattg cgttgtgcca ccggggccgg tgacgccgaa ctccgtgcgc
     4621 gcgacgtgca cacccattcc gacggcatcg atttcgagct cgtgacgccc tggggcagcg
     4681 gcgccgtgcg cagccgcctg ctcggtcgct tcaacgtcgc caacctgctc gcggtcgcgg
     4741 gcgtgctggg tgctctcgac gttccgttcg cccaaatgct gccagcgctg gaatcgctgc
     4801 agccggtggc cggccgcatg agccggatcg gcggcggtgc actgccgctg gtcgtcgtcg
     4861 actattcgca tacgccggac gcgctggagc aggcgttgct cacgctgcgc gcgcattgcg
     4921 aaggaaaact catctgcgta ttcggttgcg gcggcgagcg cgacgcgggc aagcggcccg
     4981 aaatgggcgg gatcgccgag cggctcgcgg atctcgcgat cgtcaccgat gacaatccgc
     5041 gcggcgagga tggcgatgcg atcgtcgccc agatccttgc cggcatgggt tcgccggagc
     5101 gcgcgatcgt cgagcgcgat cgtgcgcacg cgatcgatct cgcgctcgct cgtgcaagcg
     5161 aaggcgacgt ggtgttgctc gcgggcaagg ggcacgaggc gtaccaggaa agcgccggcg
     5221 tgaagcgggc gttcgacgac ttgcaggtgg cgcgtgccgc cctggaggcg cgcacgtgct
     5281 gaacctgcgt ctctcggaaa tcgccgtctg gacccgcggg agcctgcgcg gcgcggacgc
     5341 gtccgtcgcc gcgatttcca ccgattcccg gacgctcgcg gcgggcgacc tgttcgtcgc
     5401 gctggtgggc gaacgccacg acggccacga ccacgtcgcc gaagcggcgg cgcggggcgc
     5461 cgcggcggcg ctggtatcgc ggccggtcga taccgacctg ccgcaggtcg tggtcgccga
     5521 cacactgcac gcgctcggcg acctcgcgag cgcggtgcgc gcgcgacgcg acgtcaccgt
     5581 ggtcggcatc accggttcga acggcaagac cagtgtgaaa acgctgaccg cgcagatcct
     5641 cgcgcggcat ggccataccc acagcaatgc cgccagcttc aacaacgaga tcggcgtgcc
     5701 gttgacgctg ctcgcgatgc ccgacgacac cgaatacgcg gtactggaaa tgggcgccgg
     5761 caagccgggc gacatcgatt acctcgccgc gatcgtccgg ccccggatcg gcctggtcaa
     5821 caacatcggg cccgcgcacc tcgagcggat gcattcgctg gaaggcatcg ccgaaaccaa
     5881 gggcgcgatc taccgggcgc tgccgcccga cggtgtggcc gtgatcaatg ccgatgacgc
     5941 gttcgcggcc tatttcgccg ggcttgcggg tgcgcgccgc gtgctgcgtg tcggcctcga
     6001 aaagcccgcc gacatcacgg ccgaagactt gaagctcggt ccggacgcga gcggattcac
     6061 gttggtcacg ccggtcggcc gcgccgcagt gcggctcccg ctgcccgggc accacaacat
     6121 cgccaatgcg ctggcagcaa ccgcgctcgc gcaggccgcc ggcgccgcgc tggacgacat
     6181 cgtggccggg ctgcaatccg cggaatcgat cggcggccgg ttgttgcgac atgcatcgcc
     6241 cgaaggctgg gtgctgttcg acgacagtta caacgccaat ccgggctccg cgttggcggc
     6301 ggtcgcgacg ctcgccctgc aaccgggcga acgctggctg gtgctcggcg acatggccga
     6361 actcggcgcg ggcgccggcg acctgcacgc caagatcggc gcggccgcgc gcgagcaggg
     6421 catcgcgcgg gtgtacgccg tcggcgcgtt ggccgcgcgt gcggccgagg cattcggcca
     6481 aggcggttcc gtgcaccccg accagtctgc gctcgttgcg gcgttgcgcg gcgacctgca
     6541 cgcgggcgca agcgtgctgg tcaagggctc gcattcgtcg cacatggaga gggtggtcgc
     6601 cgcgttgctc gcgaacgacg aggggggcga ccggcatgtt gcttgagctc gcccattggc
     6661 tggagcagta cttcggtccg ttccggctgt tcgactacat caccttccgc acgatcatgg
     6721 cggcgttgac ggggctgttt ttttcgttgt tcttcggacc cgcgatgatc cgccggctga
     6781 ccgcgctgaa ggccggacaa gtcgtgcgca cggatggccc gcagacgcac ctggccaagg
     6841 caggcacgcc gacgatgggc ggggcgctga tccttgtcgc gatcgtggtg ggcaccatcc
     6901 tgtggggcga cctgcacagc cgctatttgt gggtcgtgct ggtcgtgacg ctcgcctgcg
     6961 ggctgatcgg tttctacgac gattaccgca agctggtatt gaaggacccg cacgggctga
     7021 tcgcgcgctg gaaatacctc gcgcaatcga tcgtgggcct cggcgcggcg ctgttccttt
     7081 tccatacggc ggacgtgccg gcggcgacgg cgctgtacgt gccgctcttc aagcatgtcg
     7141 tcattccgct cggggccggc ttcgtcgtga tggcgtacct gtggatcgtc ggcttctcga
     7201 acgccgtgaa cctgaccgac gggctcgacg gactcgcgat catgccggcg gtgctggtga
     7261 cggcggcgct cggcgcgttc gcctacctcg cgggcaacgc ggtgttctcc gcctatctcg
     7321 ggatcccctc gatcccgggc gcgggcgaac tcgcggtctt ctgtggtgcg ctgaccggcg
     7381 cgggcctcgg cttcctgtgg ttcaacacct atcccgcgca ggtgttcatg ggcgacgtcg
     7441 gtgcgctcgc gatcggcggc gcgatcggct gcgtcgcggt gatcgtgcgc caggaaatca
     7501 tcctgctggt gatgggtggg ctgttcgtgg tcgaaaccgc ttcggtgatg ctgcaggtcg
     7561 gcagcttcaa gctgaccggc cggcgcatgt tccggatggc acccatccac caccatttcg
     7621 aattgaaagg ctggcccgag ccgcgcgtga tcgtgcgctt ctggatcatt tcggttgtgc
     7681 tggtgctggt gggtttggcg acgttgaagg tgcggtgatg aaagaactgc aaaagcggga
     7741 acggggaacc gggaacaggg aggatggttc ccgcgcaaat tctattcccc gttccccgtt
     7801 ccccgttccc gccctccagg ggcccgacgc atgatgtcct tgttcgcgcc ctcccaagcc
     7861 acccgccgcg gcggtccgcg cggtcggcac gatccgtggt tgctgctggc gatcgtcgga
     7921 ttggccgcgt tcggaatcgt gatggtggct tcgagttcga tcgcggtggc cgacggccac
     7981 cacatcggcg agttctatta cctccagcgg cacgtcacct tcctcgcgct gggcggggcg
     8041 attgcgctgt ggatcgcgct caggatcgaa ctgaacacgc tggagcgctg gtcgggctgg
     8101 ttgatggtgt tcacgttcgc actgctggtc ctggtgttca ttccgcacat cggcatgcgc
     8161 atcaacggcg cgcggcgctg gatcaatctc gggatctccg ggttccagcc ggtcgaactc
     8221 gccaagttga cggtggtgct gtacgtcgcg agctacctgg tgcgccaccg cgacagcgtg
     8281 gaaacgaagt tcctgggtgc ggtcaagccg gtggcggtcg cgggattcac cgcggtgctg
     8341 ctgctcgcgc agccggactt cggctccgcg gcgctgatcg gcgtggtgac gatcggcatg
     8401 ctgtggctgg gaggcgcgcg gatgcgcaac ctcgtgatca tggggctgcc gctggtgccg
     8461 ctgggctcgt ggctggcgct gtcgaccgac taccgcgtga aacgcctgac ctcgttcctg
     8521 gatccatgga aggacccgtt caacgatgga ttccagttga cgcaggcctt gatcgcggtc
     8581 ggacgcggca agtggttcgg cgtcggcctc ggcggcagcg tccagaagct tttctacctg
     8641 cccgaagcgc ataccgattt catcctcgcg gtgatcgcgg aagaactcgg cttcgtcggc
     8701 gtgctgttcg tgctcgcatt gttcgcgttg ctcgtgggcc gcggcctgca actcggcctg
     8761 cgcggcgtgg aagtcggcca gcaccgcgcg ggctacgtcg catccgggat ttcgctgatg
     8821 atcgggctgc aggcgctggt gtcggtgggc gtgaacctcg gcgtcctgcc gaccaagggc
     8881 ctgaccctgc cgctgatcag ttcgggcggc tccagcacct tgatgacctg catcatgctg
     8941 ggcgtgctgg tgcgcgcggg ctacgaaatc catcgcgccg aggatgcgcg gcggatggcg
     9001 acccgccggg cgccggggcc gatcaccgac gacattccca tcgagaagcc ggctgccgag
     9061 cttccgctcg ccgcgcgcaa gcgcatcgag ccccgcctgg acgaggtgcg cgcatgagcg
     9121 acgcgcgggc gcccgtgctg atcatggccg gcgggaccgg cggccacatc ttccccggcc
     9181 tcgcggtggc ggcggcactg ggcgcgcgcg aagttccggt ggtctggctc ggcgcacgcg
     9241 gcgggatgga aaaccgcatc gtgcccgaac accggatcga aatgcgcacc gtgccggtcg
     9301 cgggcctgcg cggaaaggga gcggcggtgc gtctcgcggc gccctggatg ctgttgcgtg
     9361 cgatcctcgc cgcgtggcgg gtcctgcgcg acatccggcc gcgcagcgtc ttgtcgctgg
     9421 ggggctacgc cgccgggcca ggcggcatcg ccgcgtggtt gcagcgccgg ccgttgctgg
     9481 tgcacgaaca gaaccgcgtg cccggattca ccaaccgcat cctcgcgaaa ttcgcgcgca
     9541 aggtgatggg cggctttccc gacgcattcg cgccgggcgt ccactcggag tggaccggca
     9601 acccggtgcg tagcgaaatc accgcgttgc ctgggcctgc ggcgcgcttc gcggatcgcg
     9661 cgggcgtccc gcgcctgctg gtgctgggcg gcagcctcgg cgcgcgcacc ttgaacctgg
     9721 gcttgcccga ggcgttgtcg ctgatcccgc gcgaccgccg ccccgaggtg tggcaccagt
     9781 gcggcgaggc gcacctcgag accacccgca ccgcgtatgc gggcgtggac gtacacgcga
     9841 aggtggcccc cttcattttg gacatggcgg cggcgtatgc gtgggcggac ctggtggtat
     9901 gccgcgccgg tgccctgacg ctcgcggaac tcgcggccgc gggactcggc gcgatcctca
     9961 tgccgttccc gtacgcggtc gacgaccacc agacgaaaaa cgccgaaggc ttcgtcgccg
    10021 ccggcgccgc ggagttggtg caggaccgtg acttcgatcc tgcgcggttc gcggcgctgc
    10081 tggagcgcct gctcggcgac gccgccaagc ggctcgcgat ggcgaaggcc gcacgcgtcc
    10141 tcgcgaaacc ggatgccgcc gacgtgatcg cgcagcgttg cctggaggtg gccgcatgac
    10201 gccgcaccgc ctgccgccgc acggggacat catgcagggc ttccgccacg tgcatttcat
    10261 cggcatcggc ggcgccggca tgagcggcat cgccgaggtg ctgcacacgc tgggctatga
    10321 cgtctccggt tcggaccgtg cgcaatcgcc ggtcaccgag cggctggtga agatgggcgt
    10381 ggatgtcgcg atcggccacg ccgccgaaca catcaggaat gtcgacgcgg tcgtggtgtc
    10441 gagcgcgatc aagccggaca acccggaact catcgctgcg cgcgagcgcc gcatcccggt
    10501 ggtgccgcgc gcggaaatgc tgggcgagct gatgcgcttc cggcgcggca tcgcggtggc
    10561 cggcacccat ggcaagacca cgaccacgag cctcgtcgcg agcgtgctcg ccgaagccgg
    10621 ctacgacccg accttcgtga tcggcggaca actcctgtcc gccggcgcgc acgcgcggct
    10681 cggcgcgggc gattacctgg tcgccgaggc cgacgaatcc gacggctcgt tcctgatgct
    10741 ttcgccggtc gcggcgatcg tcaccaacat cgatgccgac cacctcgagc actaccacgg
    10801 cgactacgcc gagttgaagc aggcgttcgt cgatttcctg catcgcctgc cgttctacgg
    10861 cctcgcggtg ctgtgcgtgg acgacccgga cgtcgccgaa ctcgcccagc gcacgccacg
    10921 ctcgacgcta acctacgcga tcgatcgcgc ggccgacgtg cgcggtgcca atctgcgcgc
    10981 ggaaggtccc tgcatgcgct tcgacctgca gttgcccggc cagccgccct tgccggtcac
    11041 gctgaacctg ccggggcgcc acaacgtcct caacgcgctg gccgcggcga cgctcggttg
    11101 gcacctcggc atcgagccgt tcgcgatcgc ccgcgcgctg gaacgcttcg agggcgtcgg
    11161 tcggcgcttc cacatcaacg gcgagctggc ggtcgacgac ggtcgggtga tgctggtcga
    11221 cgactacggc caccacccgc gcgaaatcgc ggcggtgatg gaggccgcgc gcgcgggctg
    11281 gcccgaacgc cgggtggtga tcgtgttcca gccgcaccgc tacacccgca cccgcgacct
    11341 gatggacgac ttcgcggcgg tgttgtgcga ggccgacgtg ctggtgttga ccgacgtgta
    11401 cccggcgggc gaaatgccga tcgcaaatgc tgacggccgt gcactggcgc gggcggtgcg
    11461 cgcgcggggc aaggtcgatc cggtgttcat cgaccacccg cgcgaactgc gcgaaaccct
    11521 gccggccctt ctgcacgacg gcgacctggt gctgttgctg ggggccggcg atatcggtgc
    11581 ggcggcggcg gagctggcgc ggggcgggga tctgaagacg gacggtggcg tgtgatggcc
    11641 gtgcagcctg acaagcggga acagggaaca gggaacaggg aacaggaggc gctgggcgct
    11701 tccgcttccc cgctccccct tccccgttcc cgcttcaccg atccccgctc cttcggccgc
    11761 gtagccgtcg tgatgggcgg cagttccgcc gagcgggagg tctcgctgga ctcggggcgc
    11821 ggcgtgctcg aggcgctcaa gagccgcggc gtggacgcgc atgcgatcga cggcatcccg
    11881 gcgctgctgg acgcggtgcg cgccgggcat ttcgcgcggg tgttcaacat cctgcacggc
    11941 cagcacggcg gtggcgagga cggcgtgctg cagggcgcac tggattccct gcacgttccc
    12001 tacaccggct cgggcgtgct cggctcggcg ctgtcgatgg acaaggtgcg c
//
LOCUS       DASSNH010000092        10914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1186031, whole genome shotgun sequence.
ACCESSION   DASSNH010000092 DASSNH010000000
VERSION     DASSNH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186031"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /gene="nadE"
                     /locus_tag="VFK00_03200"
     CDS             complement(<1..1167)
                     /gene="nadE"
                     /locus_tag="VFK00_03200"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET7591992.1"
                     /translation="MATLHLAFAQCDFPVGAIAANAERVRTLMRDARAGGADLLVCPE
                     LSLSGYPPEDLLLRPSFLAACRSELDTLAVEAEGLAALVGFPSSEGVLYNAAALLQEG
                     RVAQTARKSVLPNYGVFDDKRYFEPARAATVATLKDARIGVLICEDVWQPESVAAAAR
                     AGAELVVVVNASPYDVAKQARREAMLTARAKETGCAMAYLNMVGGQDEVVYDGASLLV
                     NGDGTVAARAPAFVDALLWADFDAASRRWTARDWPTAPDASPEATLYAALVRGVRDYA
                     GKNGFEGVLLGLSGGIDSALTLAIAVDALGAENVTAVMLPSRYTSELSLREAHAQAQT
                     LGVEYFDLPIPGMVDAATAALAPACGDLGDVTAQNLQARSRGVLLMAMSNQTGKL"
     gene            complement(1240..2115)
                     /gene="sucD"
                     /locus_tag="VFK00_03205"
     CDS             complement(1240..2115)
                     /gene="sucD"
                     /locus_tag="VFK00_03205"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET7591993.1"
                     /translation="MSVLVNKNTKVICQGFTGHQGTFHSEQALEYGTKLVGGVTPGKG
                     GSEHIGLPVFNTVREAVDDTGADASVIFVPPPFAADAILEAAASGIRVIVAITEGIPV
                     LDMLRVKNVLKDYPDTVLIGPNCPGIITPGECKIGIMPGHIHKPGKVAIVSRSGTLTY
                     EAVYQTTNVGLGQSTVIGIGGDPINGMNFIDCLTLFQNDPQTEGIIMVGEIGGSAEEE
                     AAEFIEEYVGKPVVAFIAGASAPPGKRMGHAGAIISGGKGTAAAKFAALEKAGVTTVK
                     SPADLGATLARKMKH"
     gene            complement(2200..3366)
                     /gene="sucC"
                     /locus_tag="VFK00_03210"
     CDS             complement(2200..3366)
                     /gene="sucC"
                     /locus_tag="VFK00_03210"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET7591994.1"
                     /translation="MNFHEYQAKQLFAEFGIAVPPGKVAATPAEAVEAARALGGEQWM
                     VKAQIHAGGRGKSGGVKFCKSLDEVKSAAAGMLGKDMATYQSAGRALPVNLVLVTEAT
                     DIAKELYLSTLIDRGEKAVAFIASARGGVDIEQVARETPDDIHTVTVNFTEGLQPYQC
                     RELGFAMGLSGKQVNQLTKIMLGLYRLLNAKDLSLIELNPLAIVKSGDLVALDGKVNS
                     DDNAEFRHPELAAMRDVSQEDKAEAEAAAEHLNYVTMDGSIGCMVNGAGLAMATMDVI
                     KLAGGEPANFLDVGGGATKERVTAAFKLILSSPKVECILVNIFGGIVRCDLIAEGIIA
                     AVKEVGLKIPVVVRLEGTNVEKGRELLSGSGLKITPATDLNDAAQKAVAAAKAA"
     gene            complement(3540..4034)
                     /gene="lspA"
                     /locus_tag="VFK00_03215"
     CDS             complement(3540..4034)
                     /gene="lspA"
                     /locus_tag="VFK00_03215"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7591995.1"
                     /translation="MNKLAKPNALAWLVVSAATIALDQVTKAVARDTLELYLPQPVIP
                     HVLNWTLAFNRGAAFSFLAGGSGWQTWLFGLLAVAVSIGLIAWLAFTRRGDWRTALPI
                     ALIVGGALGNLIDRVVHGRVTDFIEVYWRTWSWPTFNVADSAISVGAVLLIVFGLFAG
                     RQQR"
     gene            complement(4091..4699)
                     /locus_tag="VFK00_03220"
     CDS             complement(4091..4699)
                     /locus_tag="VFK00_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET7591996.1"
                     /translation="MHAFLALGDSYTIGEGVPGDRTWPSLLAARLRSEDIEIGEPRIV
                     ATTGWTTDELQAAMDAAALTPPHALVTLSIGVNNQYRGRPVDEYRGQFGALLGRAVAL
                     AGGAPTRVVVVSIPDWGGTPFAAREGREAASIARDIDAFNAAAHAEADDAGAHWVDVT
                     MVSRAPAARSELAADGLHPSSAQYARWVEVILPVARKALRGD"
     gene            4923..5984
                     /gene="rsgA"
                     /locus_tag="VFK00_03225"
     CDS             4923..5984
                     /gene="rsgA"
                     /locus_tag="VFK00_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7591997.1"
                     /translation="MATASQQSASALQRIGWQGDLPAISGHACIARVSAQHRAGYELD
                     DGDHTFNAQPAPKFLKRNLGPEERPAVGDFVLVEPGTPPHITEVLARRSALTRAAAGE
                     RYARQVIATNVDVVLVLTGLDGDFNPARIERYLALIEGSGARAAVVLTKRDQREPAEV
                     DEAVEALRKRLPPGTPIHPVNGKDAESVAVLDSLLAPRTTAALVGSSGSGKSTLTNSL
                     LGEARVATAPTRASDSHGRHTTTHRALLRLPGGACLIDTPGMRELKLTGDEDLELFAD
                     IDELTEQCRFADCSHGNEPGCAVREALENGELDPARWRHYRKLHDEREDQAARFEDRL
                     RGTHEPIRTHRRRDWQERE"
     gene            complement(6022..8031)
                     /locus_tag="VFK00_03230"
     CDS             complement(6022..8031)
                     /locus_tag="VFK00_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7591998.1"
                     /translation="MTRALRGIVGVLLFGFAAAAVAAGQISFADLARHKQFKTVKISP
                     DGTNLAVTAVLPSGQTVLFLKNIETGKGVSLAPRGNDDVLDFWWVSPKYVVYDVAQHL
                     GGWDRPIPTGELYSVEADGQGGRMIYGYRMAGGQVGSHIQRASPERGTADFLARIDGE
                     EHDILVTVSSWEHSRALGSLSDVYRMDVRNGHKVHVATAPMRDAEFLADHHGHVRFAM
                     GDDRAGNRVIYMRPVDGGDWQLLQQASDDRDWPIAFSADDQTVWFTCPGKPNGFGICK
                     FDPATRAMTQVWSNPHVMATGLAHGLATGSTIGVEFTDGRPSISVFDNKAPAVQAIVG
                     LMQQYPGEEVSFVSGTDDGSKAIALVQADADPGTFFLYERASNTFTPVLRRADWIHPD
                     QLGRAMPFTFKTRDGLTEQGYVTYPPGKDNAKNLPMVVFVHGGPFGVRDWWEYDPYVQ
                     AMATRGYAVLQVNYRGSGGYGRAFEKAGWRQWGGAMQDDVTDATRWAIAQGIADANRI
                     CIYGGSYGGYAALEGAVEVPDLYKCAVGYVGVYDLPMLFKRGDAQQTLYGEAYMQRTI
                     GTDMTALAQRSPVYQLDNLKAKVMLVVGGRDTRVPPGQGLELHAALQKRGIAHAWLYK
                     RDEWHGFYDQGNIAELFEKVDAFLAANIGPGVESGVVAGAPAGKP"
     gene            complement(8163..>10914)
                     /gene="ileS"
                     /locus_tag="VFK00_03235"
     CDS             complement(8163..>10914)
                     /gene="ileS"
                     /locus_tag="VFK00_03235"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7591999.1"
                     /translation="GHAVNKILKDIVVRSKLMAGFRSPYVPGWDCHGLPIEIAVEKKY
                     GKVGHKIDAEAFRQHCRDYAAQQIDLQRKDFKRLGVLGDWEHPYRTMDFRYEADILRA
                     LAGVYANGHVTRGFKPVHWCFDCGSALAEAEIEYQDKQSPAIDVAYDAIDPQALAAKF
                     GVTIDDGTIVSVPIWTTTPWTLPASMAVTLGPEFDYVLVEGPARGGKRALLVVAEALA
                     EKVLARYRTESLPPPAGGGARRAHGGALGHAKGAALENLRLKHPFYDREIPLVLGDHV
                     SAEDGTGAVHTAPGHGAEDFAVGKQYGIVDATPANVLNPVGANGVYLPGTPLFEGQHI
                     WKANDAIIELLREHGVLLASERITHSYPHCWRHHTPVAFRTTPQWFIAMDAKGLRASA
                     LQAVHNDVKFYPAWGEERIAGMVEGRPDWCISRQRTWGVPIALCVHKVTGEPHPRSAE
                     LLEQVAQRVEQSGVDGWYGLDLAELLGDEARDYEKVHDILDVWFDSGVTHFCVLDQRP
                     QLHRNEGDEVMYLEGSDQHRGWFQSSLLTSCAMHGRAPFDEVLTHGFTVDAQGRKMSK
                     SLGNGIEPQDVMNRYGADILRLWIASADYRNEMALSEEILKRVADSYRRIRNTCRFLL
                     GNLEGFDPAADLLPVDRCLLLDQWAIRSARDVQDAVVAAYARYDYPEVVQRVQNFCTN
                     EMGALYLDITKDRLYTMPTASHGRRSAQSAMFRILEAMVRWLAPLFAFTSEEIWQAMP
                     RQMHMPTEIRGGFTLRDASVLYETWYQGLREAQDSPEQRRWWGDLLAIRDTAARVLEG
                     MRKDGKIGSSLDATLTIHADPAIAERYKQVADELRFFFIVSDLRLDIGDAPADAVLTE
                     LEGANVWVSAAVSEAPKCVRCWHHRDDVGRDAGHPELCGRCVVNVAAFEGRGAGEARR
                     WF"
BASE COUNT         1741 a         3827 c         3540 g         1806 t
ORIGIN      
        1 cagcttgccg gtctggttgg acatcgccat cagcagcacg ccgcggctgc gcgcctgcag
       61 gttctgcgcc gtgacatcgc cgaggtcgcc gcaggccggg gcgagggccg cggtggccgc
      121 atcgaccatc ccgggaatgg gcaggtcgaa atattcgacg ccgagcgttt gcgcctgggc
      181 gtgcgcctcg cgcagcgaaa gttcggatgt gtagcgcgac ggcagcatca ccgcggtcac
      241 gttctccgcg ccgagggcgt cgacggcgat tgcgagtgtc agcgccgaat cgatgccacc
      301 cgacaggccc agcaggacgc cctcgaaccc gttcttgccg gcatagtcgc gcacgccgcg
      361 caccagcgcc gcgtagagcg tggcttccgg cgaggcgtcg ggcgccgtcg gccagtcccg
      421 cgcggtccag cggcgcgatg cggcgtcgaa atcagcccac agcagcgcgt cgacgaatgc
      481 cggtgcgcgc gccgcgaccg tgccgtcgcc gttcaccagc agcgaggcgc cgtcgtacac
      541 cacctcgtcc tggccgccca ccatgttgag gtacgccatc gcgcagccgg tttccttcgc
      601 acgcgcagtc agcatcgcct cgcggcgcgc ctgcttggca acgtcgtagg gcgaggcatt
      661 gaccacgacc accagctcgg cgcccgcccg cgccgcggcg gcaaccgatt cgggctgcca
      721 gacgtcctcg cagatcaata cgccgatcct cgcatccttc agggtcgcga ccgtggcggc
      781 gcgtgccggt tcgaaatagc gcttgtcgtc gaacacgccg taattgggca gcacgctctt
      841 ccttgcggtc tgcgcaaccc gcccctcttg caacagcgcg gcggcgttgt aaagcacgcc
      901 ctcgctgctg ggaaagccga ccagcgcggc gagcccttcg gcttccaccg ccagcgtgtc
      961 gagctcggac cggcaagccg cgaggaagct cgggcgcagc agcaggtctt ccggcggata
     1021 gccgctcaag ctcagttccg ggcacaccag gagatcggcg cctcccgcgc gcgcgtcgcg
     1081 catcaacgtg cgaacgcgtt cggcgttggc cgcgatcgcg ccgaccggaa agtcgcattg
     1141 ggcaaaggca agatgcaggg tcgccatgga gcgttccagg aaacgcgaag gccgcgacgg
     1201 gcgcggcctt cattttaccg ttgtgcgtac tgaaccggct cagtgcttca tcttccgggc
     1261 cagcgtggcg ccgaggtcgg ccggggactt gaccgtggtg accccggcct tctccagcgc
     1321 cgcgaacttg gccgcggccg tgcccttgcc gccggagatg atcgcgccgg cgtggcccat
     1381 gcgcttgccg ggtggggccg aggcgccggc gatgaatgcg accaccggct tgccgacata
     1441 ctcctcgatg aactccgcgg cttcttcctc ggcgctgccg ccgatctcgc cgaccatgat
     1501 gatgccttcc gtctgcggat cgttctggaa cagcgtcagg caatcgatga agttcatgcc
     1561 gttgatcggg tcgccgccga tgccgatgac cgtggattgg ccgagcccca cgttggtggt
     1621 ctggtaaacc gcctcgtagg tcagcgtgcc ggagcgggac acgatcgcga ccttgcctgg
     1681 cttgtggata tggcccggca tgatgccgat cttgcactcg cccggggtga tgatgcccgg
     1741 gcagttcggc ccgatcagga cggtgtcggg atagtccttc agcacgttct tgacgcgcaa
     1801 catgtcgagc accggaatgc cttcggtgat cgcgacgatc acgcggatgc cggacgcggc
     1861 cgcttcgagg atcgcgtcgg ccgcgaacgg cggcggcacg aagatcaccg aggcgtcggc
     1921 gccggtatcg tcgacggctt cgcgcaccgt gttgaagacc ggcaggccga tgtgctcgct
     1981 gccgcccttg cccggggtga cgccgccgac cagcttggtg ccgtattcga gcgcctgttc
     2041 ggaatggaag gtgccctggt ggccggtgaa gccctggcag atgaccttgg tgttcttgtt
     2101 gacgagcacg gacatgtttt tgatcctgtt ttttgtagga gcgcgccatg cgcgcgatcc
     2161 acatgcagca ttcggtcgcg gccgtgggcc gctcctacat catgctgctt tcgcggcggc
     2221 gacggccttc tgcgcggcgt cgttcaaatc ggtggcgggc gtgatcttga ggcccgaacc
     2281 actcagcagt tcccggccct tttcgacgtt ggtcccttcg aggcgcacca ccacgggaat
     2341 cttcaagccc acttccttca ccgccgcgat gatgccctcg gcgatcagat cgcagcggac
     2401 gatgccgccg aagatgttga cgaggatgca ctcgaccttg ggcgaggaca ggatcagctt
     2461 gaacgccgcg gtcacgcgct ccttggtcgc gccgccgccg acgtccagga agttcgccgg
     2521 ttcgccgccg gccagcttga tcacgtccat ggtcgccatc gcgagcccgg cgccgttgac
     2581 catgcagccg atcgatccgt ccatcgtgac gtaattgagg tgctcggccg cggcctcggc
     2641 ttccgccttg tcttcctgcg aaacgtcgcg catcgcggcg agttccggat gccggaattc
     2701 ggcgttgtcg tcggaattga ccttgccgtc cagcgccacc aggtcgccgg acttcacgat
     2761 cgccagcgga ttcagctcga tcagcgagag gtccttggca ttgagcagcc ggtacaagcc
     2821 cagcatgatc ttggtgagct ggttgacctg cttgccggaa agccccatcg cgaagccgag
     2881 ttcgcggcac tggtagggct gcaggccctc ggtgaaattc accgtgacgg tatggatgtc
     2941 gtccggcgtc tcgcgcgcga cctgctcgat gtcgacgccg ccgcgcgccg aggcaatgaa
     3001 cgcgaccgcc ttctcgccgc ggtcgatgag cgtcgagagg tacagctcct tggcgatgtc
     3061 ggtggcctcg gtgaccagca ccaggttcac cggaagcgcg cggccggccg actggtaggt
     3121 cgccatgtcc ttgccgagca tgcccgccgc ggccgacttc acctcgtcga gcgacttgca
     3181 gaacttgacg ccgcccgact tgccgcggcc accggcgtgg atctgcgcct tcaccatcca
     3241 ctgctcgccg cccagtgcgc gggccgcctc gactgcctcg gcgggcgtcg ccgcgacctt
     3301 gccggggggc accgcgatgc cgaattcggc gaacaactgc ttggcctggt actcatgaaa
     3361 attcatggaa gaccctcgtg attcctggaa ggtgccggcg acggccgatg ccaccgccac
     3421 gtgccgcgga aacaggacca cgcaggcacg acagccgcct attgttgcgg cggatggcga
     3481 gcttgtccaa tgcgaccgtc cccgatcgcg cttcgcgccg cggcacgcag cgtcttgcgt
     3541 cagcgctgct gcctgccggc gaacaggccg aagacgatca gcagcaccgc gccgacgctg
     3601 atcgccgaat cggccacgtt gaaggtgggc cacgaccacg tgcgccagta gacctcgatg
     3661 aagtccgtga cgcggccgtg cacgacgcga tcgatcaggt tgcccagtgc gccgccgacg
     3721 atcagcgcga tcggcagggc cgtgcgccag tcgcctcggc gggtgaatgc cagccacgcg
     3781 atcaacccga tgctgacggc caccgcgagc aggccgaaca gccaagtctg ccaaccggag
     3841 ccgccggcga ggaaactgaa agccgcgccc cggttgaaag ccagcgtcca gttgaggaca
     3901 tgcgggatca ccggctgcgg caggtacagt tccagcgtat cccgcgcgac ggccttggtg
     3961 acctggtcga gcgcgatcgt ggcggcggaa acgaccagcc acgccagcgc gttgggcttg
     4021 gcaagcttgt tcaatcggaa ttccttgcgg cacgcatcgc tgaagcgcgc atgatagcgg
     4081 cgcgtgcgcg tcagtccccg cgcaaagcct ttctcgcaac cggcaggatc acctcgaccc
     4141 agcgcgcata ctgcgccgac gatggatgca ggccatcggc cgcgagctcg gagcgtgctg
     4201 cgggtgcgcg tgaaaccatg gtgacatcga cccagtgcgc gcccgcatcg tccgcctccg
     4261 catgcgcggc ggcgttgaag gcatcgatgt cgcgtgcgat cgatgcggct tcgcggcctt
     4321 cgcgcgccgc gaacggcgtc ccgccccaat ccggaatcga cacgaccacc acgcgcgtcg
     4381 gggcgccgcc tgcaagggcg acggcgcgcc ccagcaaggc gccgaactgc ccgcggtact
     4441 cgtccaccgg gcgcccgcgg tactggttgt tgacgccgat cgagagcgtc accagcgcgt
     4501 ggggcggcgt caatgcggcg gcgtccatcg ccgcctgcaa ttcatcggtg gtccaaccag
     4561 tggtcgccac gatccgcggc tcgccgattt cgatgtcttc cgaccgcagg cgcgccgcga
     4621 gcagcgaggg ccaggtccgg tcccccggaa cgccttcgcc gatcgtgtag gaatcgccca
     4681 acgccaggaa tgcatgcatg gttgattgtt ccagactgct gtagtgcgaa ggccggtcat
     4741 gcacgcccgt catggtcctg tctcgtcgag cgtgcttcct gattcgtcca aacgcggaga
     4801 ggggcaagca cggatgatct caacatgaac gacgtggctg caaacgggcg cgggatcggg
     4861 catgctagcg ggccgaggcg ctcccaaaga ccttccgaac tcccgaccgg cgattctccg
     4921 ccatggcaac tgcttcgcag caatcggcat ccgcgctcca gcgcatcgga tggcaagggg
     4981 atcttcccgc gatttccggc catgcttgca ttgcgcgggt gtccgcgcag catcgcgccg
     5041 ggtacgaact ggacgatggc gaccacacct tcaacgcgca accggcgccg aagttcctga
     5101 agcgcaacct cggtcccgag gagcgtcccg cggtcggtga cttcgtgctg gtggagccag
     5161 gcacgccacc gcacatcacg gaggttctgg cacgccgcag cgcgttgacg cgcgccgcgg
     5221 ccggcgagcg ctacgcccgc caggtcatcg ccaccaatgt cgacgtcgtg ctggtcctga
     5281 cgggcctcga cggcgacttc aatcccgccc ggatcgaacg ctacctggct ctgatcgaag
     5341 gctccggcgc gcgcgccgcg gtggtgctga ccaagcgcga ccagcgggag ccggccgagg
     5401 tggacgaggc ggtcgaagcc ctgcgcaagc gactgccccc tggcacgccg atccatccgg
     5461 tcaacggcaa ggatgccgaa agcgtcgcgg tgctcgacag ccttctcgcg ccccgcacga
     5521 ccgcggcgct ggtggggtcc tccggctccg gcaaatccac gctcaccaat tccctgctcg
     5581 gcgaagcgcg cgtagcgacg gcgccgaccc gcgcaagcga cagccacggc cggcacacga
     5641 ccacccatcg cgccctgctg cgcttgccgg gcggcgcctg cctgatcgac acccccggga
     5701 tgcgcgagct caagctgacc ggcgacgagg acctcgagct gttcgccgac atcgacgagc
     5761 tgacggaaca atgccgcttc gccgactgca gccacggcaa cgagcccggc tgcgcggtgc
     5821 gcgaagcgct cgagaacggc gagctcgacc ccgcccgctg gcgccactac cgcaagctgc
     5881 acgacgagcg cgaggaccag gcggcgcgct tcgaggaccg cctgcgcggc acccacgaac
     5941 ccatccgcac ccaccgtcgc cgcgactggc aggaacgcga gtagcggccg ccgcggccgc
     6001 ggccgcggcg cctccgagcc gtcagggctt gccggcgggc gctcccgcga ctacgccgga
     6061 ctccacgccc ggtccgatgt tggcggccag gaacgcatcg accttttcga acagctcggc
     6121 gatgttgccc tggtcgtaga agccgtgcca ctcgtcgcgc ttgtacagcc acgcgtgcgc
     6181 gataccgcgc ttctgcaggg ccgcgtgcaa ctcgagcccc tgccccggcg gcacccgcgt
     6241 gtcgcgcccc cctacgacca gcatcacctt ggccttcagg ttgtccaact ggtagaccgg
     6301 cgagcgctgg gccagcgccg tcatgtcggt gccgatcgtg cgctgcatgt aagcctcgcc
     6361 gtagagcgtc tgctgggcat cgccccgctt gaacagcatg ggcaagtcgt acacaccgac
     6421 gtagccgacc gcgcacttgt acaggtcggg tacctcgacc gcgccctcga gggccgcata
     6481 gccgccatag cttcccccat agatgcagat gcggttggca tcggcgatgc cctgcgcgat
     6541 cgcccagcgg gtggcgtcgg tgacatcgtc ctgcatcgca ccgccccact gccgccagcc
     6601 ggccttctcg aacgctcggc cgtagccgcc ggagccacgg tagttgacct gcaacaccgc
     6661 gtagccgcgc gtcgccatcg cctgcacgta gggatcgtat tcccaccagt cccggacgcc
     6721 gaacgggccg ccatggacga acaccaccat cggcaggttc tttgcattgt ccttgccggg
     6781 cggataggtg acgtagccct gctcggtcag gccgtcgcgg gtcttgaagg tgaacggcat
     6841 ggcgcgcccg agctggtcgg gatggatcca gtcggcacgc cgcagcaccg gcgtgaacgt
     6901 gttcgacgcg cgctcgtaca ggaaaaaggt gcctggatcg gcgtcggcct ggaccagcgc
     6961 gatggccttg ctgccatcgt ccgtgcccga cacgaaactc acttcctcgc ccgggtactg
     7021 ctgcatcaaa ccgacgatgg cctggaccgc cggcgccttg ttgtcgaaca ccgaaatcga
     7081 cggccggcca tcggtgaact cgacaccgat cgtggatccc gtggccaagc catgggccag
     7141 gcccgtggcc atcacgtgcg gattgctcca cacctgggtc atcgcgcggg tcgccggatc
     7201 gaacttgcag atgccgaacc cgttcggctt gccgggacag gtgaaccaca ccgtctggtc
     7261 atcggcactg aacgcgatcg gccagtcccg gtcgtcgctc gcctgctgca gcagttgcca
     7321 gtcgccgccg tcgacggggc gcatgtagat cacgcggttg cccgcgcggt cgtcgcccat
     7381 ggcgaagcgc acgtggccgt ggtggtcggc gaggaactcc gcgtcgcgca tcggcgcggt
     7441 ggcgacgtgt accttgtgac cattgcgcac gtccatccgg tagacgtcgc tcaggctgcc
     7501 gagcgcccgg gagtgctccc aactgctcac ggtcaccagg atgtcgtgct cctcgccatc
     7561 gatgcgcgct aggaagtcgg ccgtgccccg ttccgggctg gcacgctgga tatgcgaacc
     7621 gacctggccg ccagccatgc gatagccata gatcatcctg ccgccttggc catccgcctc
     7681 gacgctgtac aactcccccg tggggatcgg cctgtcccaa ccgcccaggt gctgcgccac
     7741 gtcatagacc acgtacttcg gcgataccca ccagaagtcc agcacgtcgt cgttgccgcg
     7801 cggcgcgagg ctgacgccct tcccggtctc tatatttttc aggaacaata ccgtctggcc
     7861 ggacgggagc acggccgtca cggccaaatt ggtgccgtcc ggcgaaatct tgaccgtctt
     7921 gaactgcttg tgccgcgcga gatccgcgaa tgaaatctgg ccggcggcca ccgccgcagc
     7981 cgcgaagccg aacagcaaca cccccacgat gccacgcagt gcccgcgtca tgtcgcgcct
     8041 cccaatgatc gctccccatc cggtccggct gcgccgtgac cgccctggcc gatcttaaac
     8101 ccgaaaggaa aagctgcgca cacgctcccc gacgcgcgcc cccgcggtgt accgtccgcg
     8161 gcctagaacc agcgccgtgc ctcgcccgca cctcggcctt cgaacgccgc gacgttcacg
     8221 acgcagcgac cgcacaattc cgggtgcccg gcgtccctac ccacatcatc gcggtggtgc
     8281 cagcagcgca cgcacttggg cgcttcgctg acggccgccg acacccacac gttggcgcct
     8341 tcgagctctg tcagcaccgc gtcggcgggc gcgtctccga tgtcgaggcg caggtccgac
     8401 acgatgaaga agaaccgcag ttcgtccgca acctgcttgt agcgctccgc gatcgcggga
     8461 tcggcgtgga tcgtgagcgt cgcatccagc gaggagccaa tcttcccgtc cttgcgcatg
     8521 ccttccagca cccgcgcggc ggtatcccgg atcgccagca agtcgcccca ccagcgccgc
     8581 tgctcggggc tgtcctgggc ttcgcgcagg ccttggtacc aggtttcgta cagcaccgat
     8641 gcgtcgcgca gggtgaagcc accccggatt tccgtgggca tgtgcatctg gcgcggcatc
     8701 gcctgccaga tttcttccga agtgaacgcg aacagcggcg ccagccagcg caccatcgcc
     8761 tcgaggatcc ggaacatcgc cgattgcgcg ctgcgccggc catgactggc ggtcggcatc
     8821 gtgtacaggc ggtccttggt gatgtcgagg tacagcgcgc ccatttcgtt ggtgcagaaa
     8881 ttctgcacgc gctgcaccac ttccggatag tcgtagcggg cgtaggcggc caccaccgcg
     8941 tcctgcacgt cgcgcgcact gcggatcgcc cattggtcga gcagcaggca gcggtcgacg
     9001 gggagcaggt ccgccgcggg atcgaaaccc tccaggttgc cgagcaggaa tcggcaggtg
     9061 ttgcggatgc ggcggtagct gtcggcgacc cgcttcagga tctcttccga cagcgccatc
     9121 tcgttgcggt agtcggccga cgcgatccac aggcgcagga tgtcggcgcc gtagcggttc
     9181 atcacgtcct gcggctcgat gccgttgccg agcgacttcg acatcttgcg gccctgcgca
     9241 tcgaccgtga agccgtgcgt cagcacctcg tcgaacggcg cgcggccgtg catcgcgcaa
     9301 ctcgtcaaca gcgacgactg gaaccagcca cgatgctggt ccgagccttc gaggtacatc
     9361 acctcgtcgc cttcgttgcg atgcagttgg ggacgctggt ccagcacgca gaagtgcgtg
     9421 acgcccgaat cgaaccatac gtccaggatg tcgtggacct tctcgtaatc ccgggcctcg
     9481 tcaccgagca gttcggccag gtcgagtccg taccagccgt cgaccccgga ctgctcgacg
     9541 cgctgcgcga cctgttccag caattcggca ctgcgcggat gcggctcgcc cgtgaccttg
     9601 tggacgcaca acgcgatcgg cacgccccag gtgcgctggc ggctgatgca ccagtccggc
     9661 cggccttcca ccatgccggc gatccgctcc tcgccccacg cggggtaaaa cttcacgtcg
     9721 ttgtgcacgg cttgcagcgc gctggcgcgc aggcctttcg cgtccatcgc gatgaaccac
     9781 tgcggcgtgg tgcggaacgc gaccggcgtg tggtgtcgcc agcagtgcgg atagctgtgc
     9841 gtgatccttt ccgatgccag cagcacgccg tgctcgcgca acaactcgat gatcgcgtcg
     9901 ttggccttcc agatgtgctg gccttcgaag aggggcgtgc ccggcaggta cacgccgttg
     9961 gcgccaaccg ggttcaatac gttggccggg gtcgcgtcca cgatgccgta ttgcttgcct
    10021 accgcgaagt cttcggcacc atggccgggc gccgtgtgca ccgcaccggt gccgtcttcg
    10081 gcggaaacgt ggtcgcccag taccaacgga atttcgcggt cgtagaacgg gtgcttcagg
    10141 cgcaggttct cgagcgcggc acctttcgca tgaccgagcg cacccccatg tgcccttcgg
    10201 gcacctcccc ccgcaggcgg gggaagggat tcagttcggt agcgcgccag caccttttcc
    10261 gccagcgctt cggcgacgac cagcaacgcg cgcttgccgc ctcgcgccgg gccttcgacc
    10321 agcacgtagt cgaattccgg cccgagcgtc accgccatgc tggcgggcag tgtccagggc
    10381 gtcgtggtcc agatcggcac cgagacgatc gtgccgtcgt cgatggtgac tccgaacttc
    10441 gcggccagcg cctgcggatc gatcgcgtcg taggcgacgt cgatcgcggg cgactgcttg
    10501 tcctggtact cgatctcggc ttcggccagc gccgaaccac agtcgaagca ccagtgcacc
    10561 ggcttgaagc cacgcgtcac gtggccgttc gcgtacacgc ctgcgagcgc gcgcaggatg
    10621 tcggcctcgt accggaaatc catcgtgcgg taagggtgtt cccagtcacc cagcaccccc
    10681 aggcgcttga aatccttgcg ctgcaggtcg atctgctggg cggcgtagtc gcggcaatgc
    10741 tggcggaagg cttccgcgtc gatcttgtgg ccgaccttgc cgtacttctt ctcgaccgcg
    10801 atctcgatcg gcaacccgtg gcaatcccag cccggcacgt acggcgagcg gaagcccgcc
    10861 atcagcttcg agcgcaccac gatgtccttc aggatcttgt tgaccgcgtg cccc
//
LOCUS       DASSNH010000093        28360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_256924, whole genome shotgun sequence.
ACCESSION   DASSNH010000093 DASSNH010000000
VERSION     DASSNH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 28360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28360
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256924"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VFK00_03240"
     CDS             <1..1530
                     /locus_tag="VFK00_03240"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592000.1"
                     /translation="LQRTAADWLATAQAQLLRGDAAAAQRTLAHALVEHPDSVDLRRA
                     QAGACQRLGRIAEAESLLREVLAADPGDAAAAFTLARLLKEQGRTAAAAAAMRACLAD
                     PRHSRDPDLAINAIELLDDCDRKFDAAAIALAAIADNPDEPRLHAYASMLQMQVGEFE
                     QARRHYLAALERDQRAWEWHMPIGLSSAQRYASAEHPDFALFRDGLRREDLSAKARAE
                     LHFALGKAHDDVGAYENATRHLREGNAIVHRLTKWSRKDWRRMVEARLAAAPMAHRVD
                     PRDDFTPVFVVGMPRSGTTLVAELLSRHPKVCNRGELPWIANLAARPGLNGNPARVEL
                     ERAAATYIAHSRQDDGGDARWFVDKQPMNFCYLDLVLAMFPHAKIIHCQRGARDTALS
                     LWMQCFLKDVQGYSYDFGDIALVMRDHAKLMAHWVSRHSASIRTLRYEELASDPARVI
                     RDFAGWIGLPPRTDAAPVDPKSHISTASLWQARQPVHTRSVGRWRHYAAFIPELSRIP
                     G"
     gene            complement(1527..3185)
                     /locus_tag="VFK00_03245"
     CDS             complement(1527..3185)
                     /locus_tag="VFK00_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592001.1"
                     /translation="MGVADRMFEPPAPDLAKINARLQRLPPPLGAQLQLASDALAAGD
                     LPAAQPALRNALAAAPGQPDVLRLYGLLLARIGNLRAAFANFEAVLRAAPDDAIGYWQ
                     YAQVREQSGDVASALRLRELSVQRLPGSPLALADLGEHLARHRRPEQAVAYLERAANL
                     APDYAPAQLKLGDALVACGQVERGAAALRKAIALQPAFAAAWLDLVDIKTLPVTDEEV
                     SAMQALLRRVGIDDGERTAIQFALAKACEDRGLHAEAYGLFVAANARRKGEMGHWDAG
                     RFLARAEAAEQVFAAPYATAEDPHLGEQVVFIVGMPRSGSTLVEQVLASHAEVQGMGE
                     LGELAQVLAEESTRRQRRYPEWVPQATPQDWQRLGQRYLELIAPHRDGRPRFTDKMPN
                     NWQALGAIRAMLPCAHIVICRRDPLETCWSCFKQFFARGWEFTCDFDELATFWKAFDR
                     EASAWAARDPARVRELPYEALTQDAEAQTRALLEFCGLPFDPACLAPNAASRSVHTLS
                     AAQVRAPILPRAATAAAYGALLDPLRSALGLPPAGKVPGGGRAV"
     gene            complement(3189..5039)
                     /locus_tag="VFK00_03250"
     CDS             complement(3189..5039)
                     /locus_tag="VFK00_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592002.1"
                     /translation="MTESAAAAIVAALEAGEPAQAERLCSEQLQRNPDAPDLLVLLAL
                     ARWQLGMRSESLEVYGRLVRQHPGELVHWRNYATALREAGELEAAEAASANAVRLAPD
                     DPELLDWHGLLQMDLRKPAEARDTLLRAFGKSPDSPVIRIHAARACSACRDARAEHLL
                     RPWREWLPLDAPLQSELAEALKEQGDVLAATELLEDLVRRLPAEWRLRLRLASLYERA
                     NRLEEAGALLDRIEAEGVVTDALPREMREMAHQRARLAVRRRDHAAARELLERSGPHD
                     DHHCAHWFELGQICDKLGDTTAAMAALERAHAIQIADIRANHPGWFEPGARTLPNRNP
                     RVSASDYAAWPALRAPAAAESPVFVVGFPRSGTTLLEQMLDAHPRLQSMDERPFFNML
                     ATELENTTGAVVPRDLGKLDQGDCDELRKGYVILACGKVPRRWDARLVDKNPMNMLWL
                     PMIHRMFPAGKFILAVRHPCDVLLSCYMQNFMSSSLAVVCENLETLAQAYVDSMGHWL
                     YHVDLFKPDVFVSRYEDLVADSEAQTRRIAEFLELGDAQAMLGFDRRAREKGFIATPS
                     YTQVIEPINRKGMGRWQRYRRYFEPVLPILQPMLEHWGYATDSTAAAMEP"
     gene            complement(5133..8216)
                     /locus_tag="VFK00_03255"
     CDS             complement(5133..8216)
                     /locus_tag="VFK00_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592003.1"
                     /translation="MRLDRSELSKAVQTALSMGAVAAVGVAGTAFAQNTAAPQNDQQQ
                     PQTLQTIVVTGSHIRRVDLETSNPVVAVTAQQIQQTGKLTLGDVVQNLPVITGGVNNP
                     SVNNSGATGETLVGLRGLGASRTLILVDGQRVTGTFGPADLNAIPAAAVERIEILTDG
                     ASAIYGSDAIGGVINFILKDNYQGAQFQLNYGISDHDDGVRKGASFMFGQTSDKGSIL
                     AGIDYNKFDPILQSQRKFTENALSLTATNTYGGTATAPAGLTYLAQPGSGYTSFVGGS
                     SFALRNRIFLPPGALPDSNPLSQCSSLSLNSGTFASGADPSATTADYHCFGNDPATGE
                     NDRYNYASVNYLLTPQERTNAFFKGTYHLTDNIDFYGTVIHNKTSSGSQLAPAVIGTG
                     PLGFDTGFQISKDNYYNPFGVDFTSNGATWTGRLVPAGPRIFSGQIVTDQVFTGFRGN
                     LNFGDRTWTWDVGYNYSHLSRVNTNRGLPNQSILNQEAGPSFLNADGVVQCGTAAAPI
                     SLDTCTPFDPFNLNNPNTAAVIQAVSSPALNNTWNVDRIYHADVSGGLFDLPAGTMQL
                     AAGVLYRKIYTNNVIDSNLLLNPATGTCVLGTSCSAHLQGGYNVKEAYAELYIPVLKD
                     MPFFHALNVTLGDRYSKYSTFGSTSNWKVGVEWRPIDDLLLRGTVSTVFRAPGVSDVF
                     AAPGSSAPILTSDPCDHITTAGNPACQYVPTNGTFVNKLVAAHQQIKALTSGSQYAGF
                     PLGPESGKSFDAGVVYSPHFVPGLTVQADLWRIYLNNVIVPGVGAQTVLDACFNGLEV
                     FCPLITRTNATGFEGQISQILQPTANLGRLDVKGVDFAANYRLPAFSFGQFNVGLNAT
                     YLSQFKIQTAPGIEGVNQVRNGPGMFGGFGTGLGSSCPFPAGGVCFFPRVRAQGTLGW
                     QLGPWDAQWTMRYSSRFKSGSENPAEGDTALASFPVNGPAGPLVLHYGAFVYNDVTVG
                     YNIEPINTRIDVGVDNVFDKQPPLLYANNVLNANTSPEDFDVIGRYYWGRLTVKF"
     gene            complement(8512..9405)
                     /locus_tag="VFK00_03260"
     CDS             complement(8512..9405)
                     /locus_tag="VFK00_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592004.1"
                     /translation="MQTAPAKDKASAPVWQDKNATLSVTAQGRAGTAVPTGSAASVDG
                     QTRSAAAGVRLTSKPGLTAHANVREIRWSPVTSSCAEPIAGAPADPACFNGIVSDGVQ
                     RGEIGAGFAGHGLKLDLSVGQSQSGTGATSALSRRVALPRVLPAEGGADVAAPLWFRN
                     STATSISARGQVDVAPDTSVNLGASVGRVRFLPGSGLAEDDTLDQTTLSLGIEHGAVR
                     GAIVGHVLEPNLPGAALDRNQRWSGVDLGISVRLPWRGELNFGAQNVWTSGRSPLLFG
                     PANNATPDQGRVPYVQYHQDL"
     gene            9894..12779
                     /locus_tag="VFK00_03265"
     CDS             9894..12779
                     /locus_tag="VFK00_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7592005.1"
                     /translation="MAGDVHHLAVSTSQPSTVAGLLKASGTLWAATDVGGIEAAARVL
                     FGELVPGTRVQRCPAEAAVAPSASRLHIPLPDREWLVVDAGSDEPGNRPLLSAAAEMI
                     GARITALGRQNSLTESVEQLGRAERLQRALYAIADQASAANADITTMFQALHQIVGTL
                     MYAQNFYIAFYDARTDSVRFPYYADTVDADPPKPATDFPMTEILHGPTWYVIRDGKPL
                     MGSLPSIGAQVHGPFRGIGAECVDWLGVPLLRGSEVAGCVVVQSYDDAHHYDEQDKAL
                     LIYVAQHIQTALERRMAHAELEHRVGERTEALREANRVLQQQVLERQRGERLQAALFR
                     IAELASTTDSIDNFYAAVHRVVGGLLYARNFYIALLSEDGAELTFPYSVDERDRKIQS
                     RKLANGLTEYVLRHGTALLANAPEIAELRASGEVAQQGPDSVCWLGVPLVCADHTVGA
                     LAVQSYSPDHRYTLRDQELLTFVSYHIANALERVRATESLRHAYANLEQRVGERTRAL
                     ALANRDLRAQIAERERIEARLKYETLHDSLTGLPNRSLLMQRLEHALERFHADPSKAF
                     AVLFMDLDRFKVINDSVGHLIGDDLLFQAGGRIRACVKSEDVVARLGGDEFGVLLEGV
                     SDASQACRIAKRIIDDLNAPFRLAAKELFTSTSIGITLATSHYRHPEELLRDADSAMY
                     RAKADGRHRYAVFDEGLRREAVSLMEVENDLRRSLTRGEFVPYYQPIVDLADGSVLGY
                     EALMRWRHPENGLLLPAEFLGVAEDTGASETIDWQIYEQVCRDANSLAAGDGVFVSIN
                     LSPRHFRNPELDRRLLRLLADHDVSPVRIRVEVTERAMLENPPEAKRILQTLRGAGIG
                     IALDDFGTGYSSLSYLHQYPVQAIKIDRSFITGLDAGSEGGAAVIRTILAMANLLSMQ
                     VIAEGVETAAQRDLLIKMGCRVAQGFLYSSAQPLEAWVSDTVPEAPA"
     gene            12776..13564
                     /gene="mtgA"
                     /locus_tag="VFK00_03270"
     CDS             12776..13564
                     /gene="mtgA"
                     /locus_tag="VFK00_03270"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HET7592006.1"
                     /translation="MSRAAGTRAAGRRSFARRWLRRLLWLLVALVAVTWLMVLALRFV
                     PPPTSAYMLERKVSETWRGNHDFRVRYHWVPFERMAPVAPLAMVAGEDQTFPFNHGFD
                     IASIRSAIDVAQSGGRLRGASTITQQTARNLFLWSGGGFFRKGLEAWFTVLIDITWPK
                     RRVLEVYANIAELGNGIYGVEAAAQTYFHEPASRLTKRQAALLAAVLPDPRDWHAARP
                     SAYVQRRAAWIEQQMRQLGGVSYVLDKHPPRPRVKEAKNRFRKR"
     gene            13605..14033
                     /locus_tag="VFK00_03275"
     CDS             13605..14033
                     /locus_tag="VFK00_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7592007.1"
                     /translation="MRQVKHLLEGKGNAVYTITPEAPVLDAIRLMAERSVGALPVMRG
                     EALAGIVTERDYARKVILKGHSSRETPVSDIMTPAPTSVAPVTTVDECMRLCTELRVR
                     HLPVLENNKLVGIVSIGDLVKAVIDDQAAEIDHLQRYIAG"
     gene            complement(14172..14669)
                     /locus_tag="VFK00_03280"
     CDS             complement(14172..14669)
                     /locus_tag="VFK00_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7592008.1"
                     /translation="MKQFAIMIAMLGGTAVILIIIGALVWSTLPKDGREAQVKQAGDR
                     ITPVGAVYAGDTGRAAMQAAKAEATKAAASQVAYGGTTDGKVVFDKLCTTCHTTGAAG
                     APKITDKAAWAPRLKEGIDTLVKHAIDGYTGPDGNHMPAKGGNPALTDEQVEATVKWM
                     VSQVQ"
     gene            14884..16446
                     /locus_tag="VFK00_03285"
     CDS             14884..16446
                     /locus_tag="VFK00_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7592009.1"
                     /translation="MPEHYDIIVIGSGPGGASLAHRLAPSGLKILILERGDWLKRAAA
                     NWDPREVFINNIYQADETWYDKSGEAFHPGLHYFVGGNSKMYGAALFRLRERDFGELR
                     HADGLSPEWPLKYDVFEPYYDEAERLFHVHGAAGEDPTEPPRSRPYAWPPVSHEPRMQ
                     ELSDTFSRHHLHPFHMPLGILLEEQDGRVLRRSPCIRCGAFDGYPCLTNGKADAEVIC
                     IDPMLGAHDNVTLLTGAYVSRLDTDAGGHAVSTVQVERGGAKESYSANVVVVACGALS
                     TALLLLRSANDKHPDGLANGSGQLGRNYIRHNQSVLMAITRQPNDTVFQKTVGVSDFY
                     FGADDFEFPLGLIQMTGKSHPDTIRGEALPAWLEWLPEKPFEYLARHSLDFWLSSEDL
                     PRPENRIRYEGGRVVVEQTDNNMQGHRRLRAKLESLLGPMDAHPFMLERNYYLGKDVP
                     IGGTAHQAGTARFGEDPATSVLDLDCKAHELDNLYLADASFFPSIGAVNPTLTIIANA
                     LRVADRIAERMK"
     gene            16543..17055
                     /locus_tag="VFK00_03290"
     CDS             16543..17055
                     /locus_tag="VFK00_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7592010.1"
                     /translation="MWLDLYVIPILIRVCLVVLFPFSALDKIVFWKDALAQADSSFLP
                     KSSGPPLLATAIVVEAIAPFCIVVGWHDRIAALVLALFCVVTAILYHPFWKFADFWKP
                     GDSIARSHFWDFLKNFGLVGGLLLVVFATRLAPAGVIAHRPLSSTAVYTAAGTAPASA
                     VPGRTHDMQR"
     gene            17159..17599
                     /locus_tag="VFK00_03295"
     CDS             17159..17599
                     /locus_tag="VFK00_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592011.1"
                     /translation="MAHGHSNEAPTLAYWHVWVDDDGVSRQERRSLGRFEQQSMGGAA
                     PQWNAVQPAAPSKAIFTVLPVGWSGEWHENPAPQWIVPMSGRWFVETMDGHRVEMGGG
                     ELSFGGDQGCTADAEGRKGHRSGTVGDEPAVLMLVQMDDGCCRQ"
     gene            17711..18616
                     /locus_tag="VFK00_03300"
     CDS             17711..18616
                     /locus_tag="VFK00_03300"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7592012.1"
                     /translation="MSDYEILDPRFRAMVLPNAPLEKLADGFRWLEGPVWFADLRCLL
                     FSDIPNDRIMRWTASGGVSVFREPSRFANGHTRDREGRLIGCLHGGRAVVRTEHDGTV
                     TTLADRYQGKRLNSPNDVVVKSDGSIWFSDPPYGIQTDYEGGRRESELPANLYRLDPA
                     NGSLHLACGDIAGPNGLCFSPDEKRLYVTDTGIQFDPDSPRCILALDVAEDGKTLSDP
                     RDFHAVSPGHADGIRCDEDGNVWAAAGDGVHCIARDGTLLGKIKVPATVSNFCFGDRH
                     YSRLFLCASQALYAIWVNRRGAQRP"
     gene            18625..19533
                     /locus_tag="VFK00_03305"
     CDS             18625..19533
                     /locus_tag="VFK00_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7592013.1"
                     /translation="MQRLLRIERNTRDLERAIAFHRDALGFSVIDADASPPAWTQLPG
                     LDAAPSRCARLGLGEQEIEFTEFPDAAPYPHGSTSCDIGFQHCAIVVSDMEAAYRRLV
                     VEGTVPITQGGPQQLPPATGSVTALKFRDPDGHPLELIWFPEGTGDPCWKKPRSGNPA
                     TLGIDHTAISVADTGRSVLFHELVGLHVGARGVNRGIEQQRLDDLAGVEVDVIAMQAA
                     AHTPHLELLGYRTPRGRVAPPAPITAVVADRVVWQAEAVDVLLEAATDGGFADGVVAS
                     GRVRGTTIALLHDPDGHRMVLAGAAA"
     gene            19641..>28360
                     /locus_tag="VFK00_03310"
     CDS             19641..>28360
                     /locus_tag="VFK00_03310"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET7592014.1"
                     /translation="MRRAARRVAHAHARALPYEAPDDEAPPTPANILARQHGQLTSAA
                     PFDLGRALAAYDQALARAYSVARTAATDNRRVEPAAEWLIDNVYLVRNEIREVREALP
                     AKVWRRLPRQAVEGGVVVPRMLRVLRASIAQLDGNIEPDAIEDYLEAYQRRASLDLVE
                     LWTLPVLARVALIESLAAGANTIARRLEAYSNAEFWAEHLITVAARAPNDMLGSVAEM
                     ARANLFVSPAFAAEFYRLLEGKHPSLKLALGLAEQQLEQQGTSVARVIDSESRAQAAD
                     QVSIANRINSLRRITDYNWSDLIERVGTVDRILAGDPAGAYMRMDFTTRGRYRHAVES
                     LAQRSGRGEPDIARRAVELAGAERAPGSDPRTRHVGYFLVGAGRDAFARELGVRRSAE
                     YRIAARLRRLPSTTYLGSIFITTAIITAVVASVHHWYLAPLPIAADTALLVAVFVAAS
                     QPAVTLANWVLGMLVPAQLLPRMSFSGGIPDECRTLVVVPWLLGSTSTLAEQLDALEI
                     RYLGNRDPNLRYALLTDFPDAPERDMAGDATLLAAASAGIERLNAKHALPGSTRFYLF
                     HRPREWNARQSAWMGFERKRGKLGDLNRVLRGARPARFFSKVVGDLAALQGTRYVITL
                     DADTQLPPEAARKLVETMAHPLNRPWFSKGSRRVVAGYGLLQPRISVTYGAEGPSRFA
                     RLYSDATGLDPYTRAVSDVYQDLFGEASFVGKGVYDVDAFSRSVGTRFPNDLILSHDL
                     LEGSYARAGLVSDIELIEHQPNRFSVDVRRRHRWTRGDWQIVQWLLPIVPGPRRRGLR
                     NTLKAHHRWKILDNLRRGVVPLAMLALLLGGWNHSNHGAYWSLVVVAFLLGPPLLISA
                     VQVLRNLWQRVPPRRWRNTWRNIRHELLRDVFAIAVLPFETWLNLDAILLSGWRLIFT
                     RRHLLEWTPMTDAERGAASGLPGYTRLMWMAPSVAVIAGFALAFENPVVLPPALPFLV
                     LWFVAPLLAWWISRTPQHPALPLDARQRLFLHAVARRVWHWFDTFVNEDEHWLPPDNV
                     QVFPVEQVARRTSPTNIGMALLATLSACDFGYITRDGLLRRTGATLETLGKLQRHRGH
                     FYNWYGTATLEPLSPMYVSSVDSGNLMGCLLVLANALQHTDSDPLVPPNLASGLRDTA
                     QLLGELLPTMEPTAPHAMLALSATLAELDALLQDAIANASVLPVLHGLLARIESESGK
                     LGQIVNDNAIGGEFAEWVGVLARQATAACEELDELAPWLGLGDSGSDDEQARAWLVEL
                     EHNPDTARARTLAAGVVGRLEAEGAADSCAALRELQATLNRRRDDALALAAQCREFAA
                     MDLSFLWNERRRQFTIGYDVARDKRDASHYDLLASEARILSYVAIAQGQVPPTHWFQL
                     GRLLTVAAGRPTLVSWSGSMFEYLMPLLLMPNYRHTLIADTCGAAVARQIQYGAQQQV
                     PWGVSESAYNATDANLVYQYRAFGVPGLGLKRGLGEDLVIAPYATTMALMIDPAAACA
                     NLQRLAEVGALTRYGFWEALDYTAGRVSENEDFALVKSWMAHHHGMSLLAMSSLLNRQ
                     PMQQRFMREPMFRAYELLLREKIPEARPRHPATLDVEQPRQTRARVHAQTREILDMDT
                     PLPEVHLLSNGNYHVMLTQAGAGYSRWRELALTRWHGDAASDDEGLFVYLRDVDSGQV
                     WSATRQPIGDAGGTCSATFSQARAEFHRNDFGIATEMVVAVSAEDDIELRRLRLTNRT
                     LKPRTLEITSYAEIVLAPATADAAHPVFSKLFLETESAPELDALLATRRARGPDDHHP
                     WMLHQITTRGGTIGEGAFETDREAFLGRLHGPAHPQAIAGDVPLAGHAGAVLDPIFSL
                     RRRIRILPGHTAGVDVLTGVAPDRDAALALARKYHERHLHQRVFQLAWTHEQVALQQL
                     NMTAVEAQRYERIASALLYGPIAARRDTGGALPARSALWKHGISGDLPIVLARIANAS
                     QIDLVRHLIQAHGYWQMRGLHADLLIWNEELSGYRQELQEHILYLIESSAEARGGLGR
                     GRMFAWRIEHLAAPDRALMLAMARCVFSGDAGRLRDQITRLLPPGEPSRPVARTPRHK
                     PQAGSYALQLPSEDLRLSNGFGGFRNDGSEYSIWLDGDAATPAPWCNVLANADFGTVL
                     SETGGAYTWKENAHEYRLSPWYNDPLRDLSGEAAWLRDEATGEYWSLTPQPTPDGDPH
                     RVCHGFGSSRFEHVHADLYSEWTVCVAAEAPVKLSLLKLVNKSDRVRVISLTSYVEWT
                     LTENRARSTGHVRTWIDRGSGALFARNPFEDTFADRVAFHALAARPVTHCADRRAFIG
                     RNRGLAAPAAMHQALDGTVGAGLDACSGQRTVFELAPGAEIETVVLLGAAAGEHEARE
                     LVRAFADVDAAYAELERVRGDWHHAVSQLHVHTPDAATDLMVNGWLVYQVLASRIFAR
                     SGYYQSGGAWGFRDQLQDVMAVMHTRPDLAREQLLRCARHQFREGDVQHWWHPPGGKG
                     VRTRISDDLLWLPWCTAEYVRVTGDGTVLDETAPFIEGRRLAADEESAFEDVHVTDEE
                     ASLYEHCLRAIRASLKSGSHGLPLIGGGDWNDGMNRLGLEGRGESVWLGMFLHDVLTR
                     FAPLAEARGDAGSVRQFGAAAAELAAMLEHNAWDGDWYLRAWNDDGIAIGSSQSEECR
                     IDSLPQSWSVLAGIGDAARRERAMDAVLERLVDEEAGIVKLFAPPFDKAPLDPGYIKG
                     YVPGVRENGGQYTHAAIWVAMALAKLGRADDAWRIARMLNPIRHSEATEAARRYRLEP
                     YVVAADVYSAPGHLGRGGWSWYTGSAGWMYRLLTESLLGLRREGERLTFAPLVPDDWR
                     RWSADWRHGGTRYHIEFVRQPGDTGVADVSVDGALQSGHAIDLVDDGDNHEVLVRFAK
                     MEGARATEDSEQRATSRDASG"
BASE COUNT         4637 a         9700 c         9527 g         4496 t
ORIGIN      
        1 ttgcagcgca ccgcggcgga ctggctggcg acggcgcagg cgcaactgct gcgtggcgat
       61 gccgccgcgg cgcaacgaac cctggcccac gcattggtcg agcatccgga ttcggtcgat
      121 ctgcgtcgcg cgcaagcggg cgcctgccag cgactgggcc gcattgccga agcggaatcg
      181 ctgctgcgcg aagtgctcgc tgccgatccc ggcgacgccg cggcggcatt cacgctcgcg
      241 cgcctgctga aggagcaggg acggacggcc gccgcggcgg cggcgatgcg cgcatgcctc
      301 gccgatccgc ggcacagccg ggacccggat ctcgccatca acgcgatcga gttgctggac
      361 gactgcgacc gcaagttcga cgccgccgcg atcgccctcg ccgcgatcgc ggacaatccg
      421 gacgaacccc gcctgcacgc ctacgcgagc atgctgcaaa tgcaggtcgg cgagttcgag
      481 caggcgcgcc ggcattacct ggccgcgctg gagcgcgacc agcgggcctg ggaatggcac
      541 atgccgatcg gcctttcctc ggcgcaacgc tatgcgagcg ccgaacatcc ggatttcgcg
      601 ctcttccgtg acggcctgcg gcgcgaggat ctttccgcca aggcacgcgc ggaactgcac
      661 ttcgcgctcg gcaaggcaca cgatgatgtc ggtgcttacg aaaacgccac gcgccatttg
      721 cgcgaaggca acgcgatcgt gcaccgcctg acgaagtggt cgcgcaagga ctggcggcgc
      781 atggtcgagg cccggctggc ggcggcaccg atggcccatc gcgtcgaccc gcgggacgac
      841 ttcacgccgg tattcgtggt ggggatgccg cgttccggaa ccaccctggt cgccgaattg
      901 ctgtcgcgcc atcccaaggt ctgcaaccgc ggcgagttgc cctggatcgc gaacctcgcg
      961 gcgcgccctg gcctgaacgg caatcccgcg cgcgtcgagc tggaacgagc cgccgcaacc
     1021 tacatcgcgc attcgcggca ggatgacggg ggcgatgcac ggtggttcgt ggacaagcag
     1081 cccatgaact tctgctacct cgacctggtg ctggcgatgt ttccgcacgc gaaaatcatc
     1141 cattgccagc gcggcgcgcg cgataccgcg ctgtccctgt ggatgcagtg cttcctgaag
     1201 gatgtgcagg gctacagcta cgacttcggc gacattgctc tggtgatgcg cgatcacgcc
     1261 aagctgatgg cgcactgggt ctcgcgccat tccgcgtcga tccgaaccct ccgctacgag
     1321 gaactggcgt cggatcccgc gcgcgtcatc cgggacttcg ccgggtggat cggcctgccg
     1381 ccgcgcacgg atgcggcgcc ggtcgatccg aagtcgcaca tcagcaccgc cagcctctgg
     1441 caggcgcgcc agccggtgca cacacgctcg gtcggacgtt ggcggcatta cgcagcgttc
     1501 atccccgagc tgtcgcgcat tcccggctag actgcccgac cgccccccgg tactttcccg
     1561 gccgggggta aacccagcgc cgagcgcagc ggatccagca gcgcaccgta cgcggccgcg
     1621 gtagccgcgc gtgggagtat gggggcgcgc acctgcgccg cgctgagggt gtgcacgctg
     1681 cgggacgcgg cgttgggcgc gaggcaggcc gggtcgaatg gcaggccgca gaattcgagc
     1741 agcgcgcggg tctgcgcctc cgcgtcctgc gtcagcgctt cgtacggcag ttcgcgcacc
     1801 cgggccgggt cgcgcgccgc ccatgccgaa gcttcgcgat cgaatgcctt ccagaacgtg
     1861 gcgagctcat cgaagtcaca ggtgaactcc cagccccgtg cgaagaactg cttgaagcac
     1921 gaccaacagg tctcgagcgg gtcgcgccgg cagatcacga tgtgcgcgca cggcagcatc
     1981 gcccggatcg cgccgagtgc ctgccagttg ttcggcatct tgtcggtgaa gcgcgggcgg
     2041 ccgtcgcgat ggggcgcgat gagctccagg tagcgttggc cgagcctttg ccaatcctgc
     2101 ggggtcgcct gcggcaccca ctccgggtag cggcgttgcc tgcgcgtgga ttcctcggcc
     2161 aggacttgcg ccagttcgcc gagttcgccc atgccctgga cctcggcgtg cgaggccagt
     2221 acctgctcga ccagcgtgct gccggaacgc ggcatcccca cgatgaaaac cacctgctcg
     2281 ccgaggtgcg gatcttccgc ggtcgcgtac ggcgccgcga acacctgctc tgcagcctct
     2341 gcgcgcgcca ggaatcgtcc ggcatcccag tgccccatct ctcccttgcg cctcgcgttg
     2401 gcggccacga acaacccgta cgcttcggcg tggaggccgc ggtcctcgca tgccttcgcg
     2461 agcgcaaact ggattgcagt gcgctcgccg tcgtcgatgc cgacgcgacg caacaacgcc
     2521 tgcatggccg agacttcttc gtccgtgacg ggcagcgtct tgatgtcgac gaggtcgagc
     2581 catgcggcgg caaacgcggg ttgcagcgcg atcgccttgc gcagcgcggc cgcgcctcgt
     2641 tcgacctgcc cgcaggccac gagcgcatcg ccgagcttca gttgtgccgg cgcgtaatcc
     2701 ggcgcgaggt tggcggcacg ttccaggtac gccacggcct gttcgggacg ccgatggcgc
     2761 gcgaggtgtt cgccgaggtc cgccagcgcc aggggcgagc cgggcaggcg ctgcacggac
     2821 agctcgcgca agcgcagtgc ggaggccaca tcaccggact gctcgcggac ctgcgcgtat
     2881 tgccaatagc cgatcgcatc gtccggcgcc gcgcgaagca cggcttcgaa attcgcgaac
     2941 gcggcacgaa gattgccgat ccgcgccagc agcaagccat agaggcgcag cacatccggc
     3001 tggcccggcg cagccgcgag cgcatttcgc agcgccggct gcgcggccgg caaatcgccg
     3061 gccgccaatg catcactggc aagctggagc tgcgcaccca gcggtggcgg caggcgttgc
     3121 aggcgcgcgt tgattttcgc gaggtcgggc gctggcggtt cgaacatccg gtcggcaaca
     3181 cccaccgctc aaggctccat tgccgcggcg gtcgaatccg tcgcgtaacc ccagtgctcg
     3241 agcatcggct gcaggatcgg cagcaccggc tcgaagtacc ggcgatagcg ttgccagcgg
     3301 cccatgccct tgcggttgat cggctcgatc acctgggtgt agctgggggt ggcgatgaat
     3361 cccttctcgc gtgcgcgtcg gtcgaagccg agcatggcct gggcgtcccc gagctccagg
     3421 aactcggcga tgcggcgcgt ctgggcctcc gaatccgcca cgaggtcctc gtagcgcgag
     3481 acgaacacgt ccggcttgaa caagtcgacg tggtagagcc agtgccccat ggaatcgacg
     3541 tacgcctggg cgagcgtctc caggttctcg cacacgactg cgaggctgct ggacatgaag
     3601 ttctgcatgt agcagctcag caacacgtcg cacgggtgcc ggacggcaag gatgaatttc
     3661 ccggcgggaa acatgcggtg gatcatcggc agccacagca tgttcatcgg attcttgtcc
     3721 accagccgcg cgtcccagcg gcgcggcacc ttgccgcacg ccaggatcac gtaacccttt
     3781 cgcaactcgt cgcaatcgcc ctggtccagc ttgccgaggt cgcgcgggac cacggcaccg
     3841 gtggtatttt cgagttcggt ggccagcatg ttgaagaacg gccgctcgtc catcgactgc
     3901 aggcgcggat gggcatccag catttgttcc agcagggtgg tgccggagcg cggaaagccc
     3961 accacgaaga ccggcgactc ggccgcagcc ggagccctga gcgccggcca cgccgcatag
     4021 tcggaggcag agacgcgcgg gttccggttg ggcagcgtcc tggcgcccgg ctcgaaccag
     4081 cccggatggt ttgccctgat gtcggcaatc tggatcgcgt gggcacgctc gagcgccgcc
     4141 atcgccgcgg tggtgtcgcc gagcttgtcg cagatctgcc ccagttcgaa ccagtgtgcg
     4201 caatggtggt cgtcgtgggg gcccgagcgc tccaggagct cgcgcgccgc ggcatggtcg
     4261 cgccgccgca cggcgaggcg tgcccgctgg tgggccatct cccgcatctc gcggggcagc
     4321 gcgtccgtca ccacgccttc ggcttcgatc cggtccagca aagcgcctgc ctcctcgagg
     4381 cggttcgccc gctcgtacaa cgacgccagc cgcaggcgca accgccactc cgcgggcaac
     4441 cgacggacaa ggtcctccag cagctccgtc gccgccagca cgtcgccctg ctccttcagc
     4501 gcttccgcca gctcggactg gagaggagca tcgagcggca gccactcccg ccacggccgc
     4561 agcaggtgct cggcccgcgc gtcgcggcag gccgagcaag cgcgcgccgc atggatacgg
     4621 atgaccgggg aatccggcga cttcccgaat gcgcgcaaca acgtgtcgcg cgcttcggca
     4681 ggcttgcgca aatccatctg cagcaggccg tgccagtcca gcagttccgg atcgtcgggc
     4741 gccagccgta ccgcgttcgc cgatgccgcc tccgcggctt cgagctctcc ggcctcgcgc
     4801 aaggcggtcg catagttccg ccagtggacg agttcgccgg ggtgctgacg aaccaggcgc
     4861 ccatagacct cgagcgactc gctgcgcatt cccaactgcc accgcgccag cgccagcagc
     4921 accagcaggt cgggcgcatc ggggttccgc tgcagttgct cgctgcagag acgctcggct
     4981 tgcgccggtt cgccggcctc caacgcagcg acgatcgcgg cggccgcgga ttccgtcatc
     5041 gtggacacgc gaaaaagaaa agggccgcac ggaggatacc cgtgcggccc ggaagggtga
     5101 agcttggtcg agcctgatca ggccatgccg gatcagaact tgacggtcag gcgtccccag
     5161 tagtaacggc cgatgacgtc gaagtcctcc ggggaggtgt tcgcgttcag cacgttgttg
     5221 gcatacagca gcggcggctg cttgtcgaac acgttgtcca cgcccacgtc gatgcgggtg
     5281 ttgatcggtt cgatgttgta gccgaccgtc acgtcgttgt acacgaacgc gccgtagtgc
     5341 agcaccagcg gaccggcagg cccgttcacc gggaacgatg ccagcgcggt gtcgccctcg
     5401 gccggattct cggaacccga cttgaacctg ctggagtagc gcatggtcca ctgtgcatcc
     5461 cacggaccca actgccagcc gagggtccct tgcgcgcgga cccgcgggaa gaagcacacg
     5521 ccgcccgccg ggaacgggca ggagctgccc agcccggtgc cgaacccgcc gaacatgccg
     5581 gggccgttgc gcacctggtt gacgccttcg atcccgggtg ccgtctggat cttgaactgg
     5641 ctcaggtagg tcgcgttcag gccgacgttg aactggccga aggagaacgc aggcagccgg
     5701 tagttggcgg cgaagtccac gcccttgacg tctagccggc ccaggttggc ggtcggctgg
     5761 aggatctggc tgatctggcc ctcaaacccc gtggcgttgg tacgggtgat caacgggcag
     5821 aaaacctcca ggccgttgaa gcacgcgtcg agcacggttt gcgcaccgac acccggaacg
     5881 atcacgttgt tgaggtagat gcgccacagg tccgcctgca ccgtcaggcc cggcacgaaa
     5941 tgcggcgaat agaccacgcc cgcatcaaac gacttgccgc tctccggccc cagcgggaag
     6001 ccggcatact gcgaacccga cgtcaacgcc ttgatctgct gatgcgccgc gacgagcttg
     6061 ttgacgaagg tgccgttggt tgggacgtat tggcacgctg gattgccggc cgtggtgatg
     6121 tggtcgcacg gatccgaggt caggatcggc gccgaactgc ctggcgcggc gaacacgtcg
     6181 ctgacaccgg gggcacggaa gaccgtcgag accgtgccac gcaggagcag gtcgtcgatc
     6241 ggacgccact cgacgcccac cttccagttg ctggtgctgc cgaaggtgct gtacttggag
     6301 tagcgatcgc ccagagtcac gttcagggca tggaagaacg gcatgtcctt gaggaccggg
     6361 atgtacaact ccgcgtacgc ttccttgacg ttgtagccgc cctgcaggtg cgcgctgcag
     6421 gaggtgccca gcacgcaggt gccggtcgcg ggattcaaca acaggttcga gtcaatgacg
     6481 ttgttggtgt agatcttgcg gtacaagaca ccggcggcca actgcatggt gcccgccggc
     6541 aggtcgaaca accctccgct gacatcggcg tggtagatcc ggtcgacgtt ccaggtgttg
     6601 ttcaacgccg gggacgatac cgcctggatc accgcagccg tattgggatt gttgaggttg
     6661 aacggatcga acggcgtgca ggtgtcgagc gagatcgggg ctgctgccgt accgcactgc
     6721 accacaccgt ccgcattgag gaacgacgga ccagcctcct ggttcaggat gctctggttc
     6781 ggcaggccgc ggttggtgtt gaccctcgag aggtggctgt agttgtagcc cacgtcccac
     6841 gtccaggtgc ggtcaccgaa gttgaggttg ccgcggaagc ccgtgaacac ctggtcggtg
     6901 acgatctgcc cggagaagat gcggggaccc gcgggaacca ggcggccggt ccaggtggca
     6961 ccattcgagg tgaagtccac tccgaagggg ttgtagtagt tgtccttgga aatctggaag
     7021 ccggtgtcga agccgagcgg accggtgccg atcaccgccg gcgcgagctg gctgcccgaa
     7081 ctggtcttgt tgtggatgac cgtgccgtag aaatcgatgt tgtcggtgag gtggtaggtg
     7141 cccttgaaga acgcgttggt gcgctcctgc ggggtcaaca ggtagttgac cgacgcgtag
     7201 ttgtaacggt cgttctcgcc ggtggcggga tcgttaccaa aacagtggta gtcggcggtg
     7261 gtcgcactgg gatccgcgcc ggaggcgaac gtgccgctgt tcagggacag gctgctgcac
     7321 tgcgacagcg ggttgctgtc ggggagtgcg cccggcggca ggaaaatgcg gttgcgcaac
     7381 gcgaacgacg agccgccgac gaacgaggtg tagcccgaac cgggctgcgc caggtaggtc
     7441 aggccggcag gcgccgtggc cgtgccgccg taggtgttgg tcgccgtcag cgacagggcg
     7501 ttttcggtga acttgcgctg cgactgcagg atcgggtcga acttgttgta gtcgatgccg
     7561 gccaggatgc tgcccttgtc ggacgtctgg ccgaacatga agcttgcgcc cttgcgcacg
     7621 ccgtcatcgt gatcggagat gccgtagttg agctggaact gggcgccctg gtagttgtcc
     7681 ttcaggatga aattgatgac gccgccgatc gcatccgacc cataaatggc cgacgcgccg
     7741 tcggtcagga tttcgattcg ttccaccgcg gcggccggaa tcgcgttcaa atccgcggga
     7801 ccgaaggtgc cggtgacacg ttggccgtcg accaggatca gggtgcggct cgcgcccagg
     7861 ccgcgcaggc cgacgagagt ctcgccagtg gcgccgctgt tgttgacgct ggggttgttc
     7921 acgccgccgg tgatgaccgg aaggttctgc acgacgtcgc cgagcgtcag cttgccggtc
     7981 tgctggatct gctgcgccgt gacggcaacc accgggttgg aggtttccag gtccacgcgg
     8041 cgaatgtgcg aacccgtgac cacgatagtc tgcagggtct gcggttgttg ctgatcgttc
     8101 tgcggggcag ccgtgttctg cgcgaaggca gttccggcaa cgcccacggc ggcgacggca
     8161 cccattgaaa gggccgtctg cacggccttg gacagctcac tgcggtcgag tctcattgca
     8221 ctctccagac gtcaaaaaaa tgttcgaaca gttcgggaca aattccccat caggtggccc
     8281 ggtccacgcc ctctagcttt ttttgccggc gatctgcccg cttgggcagg cgccgatgcc
     8341 gcgatgctac cgcacttcgt aaaaagggtg ttaacgccgc ttggcacccc ggtcagtgac
     8401 cattcagtgc gagttccccc tggcactgaa aacaggcaga cactgtccgt cgaaggcacg
     8461 cggcgggcca tgcgcgtgtg ccccgaagtc cggacgttga accttaaccg atcaaagatc
     8521 ctggtgatac tgcacgtacg gcacccggcc ctggtcgggg gtggcattgt tcgccggccc
     8581 gaacagcagc ggcgatcggc cggaggtcca cacgttctgg gcaccgaaat tcagctcacc
     8641 ccgccaaggc aatcgcacgc tgatgccgag atcgacgccg ctccagcgct ggttccggtc
     8701 cagcgccgcc ccgggcaggt tcggttcgag cacgtgcccc acgattgcgc cgcgcaccgc
     8761 gccgtgctcg atgccgaggc tgagcgtggt ctggtcgagc gtgtcgtcct cggccagccc
     8821 ggaacccggc aggaaccgga cgcgtccgac actggcgccg aggttcacgc tggtatcggg
     8881 cgcaacatcc acctggccgc gcgcgctgat gctggtcgcg gtgctgttgc ggaaccacag
     8941 cggggccgcg acatcggcgc cgccttcggc cggcagcacc cgcggcagtg ccacccggcg
     9001 tgacagcgcg gacgtggcgc cggtcccgct ttgcgattgg ccgaccgaca gatccagctt
     9061 gaggccgtgg ccggcgaacc cggccccgat ttctccgcgc tgcacgccgt cgctcacgat
     9121 gccgttgaaa caggccggat cggccggtgc gcctgcgatg ggttccgcgc aactggacgt
     9181 caccggcgac cagcggatct cacggacatt ggcgtgggcg gtcaggcccg gcttggaagt
     9241 gaggcggacg cccgcggcag cggaacgggt ctgcccatcc actgacgcgg cagatcccgt
     9301 gggcaccgca gtgccggcac ggccttgcgc cgtgaccgac agcgtggcgt tcttgtcctg
     9361 ccagaccggc gcactggcct tgtccttggc gggcgcggtc tgcacgggcg gaataacggg
     9421 catcggcggc gtttgcgcaa gcgccaaccc cggcagggcc aagcccaagc cggctagcaa
     9481 cgcattggca agtatccgac gcatctccga agacccaatt cccgtagccg cagcccagcc
     9541 gacgcgacgg cgccgacttt gggctctagt gtacgccacc tgattacact ttgctaacaa
     9601 ttgctgcacc gcccggtgcg gcatttatcg agggttttcc caaagcggtg gcaaagcccc
     9661 ttgccgcgga gaacgcggat cggtgccctt cgtccgtgtt cgcccgatcc gtgccgggaa
     9721 tcgccttttc ccactgccaa aagggccatg cttcacgcag gcatcagcaa gtcgcgtgcg
     9781 gcggccgccc ggctaccgcg cgcaagtcgc cgagactggc gttttcccca cgccagcacg
     9841 acttttgcat gacgtcgcgg ttacactctg aactgcgagg ggttggacca tccatggcag
     9901 gcgacgttca ccatctggcc gtttccacga gccagccgtc caccgtagct ggcttattga
     9961 aggccagcgg cacgctgtgg gcggcgacgg acgtcggcgg gatcgaagcg gcggcgcgcg
    10021 tcctgtttgg cgaactggtc ccgggaacgc gtgtccagcg gtgtccggcg gaagccgccg
    10081 tagcgccttc cgcgtcgcgc ctgcacattc cgctaccgga ccgcgagtgg ctggtggtcg
    10141 atgccggcag cgacgagccc ggcaaccgcc ccttgctttc ggcggcggcg gagatgatcg
    10201 gcgcccgcat cacggcgctc ggcaggcaga actcgctcac agaatcggtc gaacaactgg
    10261 gccgcgccga gcgcctgcaa cgcgccttgt atgcgatcgc cgaccaggcc agcgcggcca
    10321 acgccgacat cacgacgatg ttccaggcgt tgcaccagat cgtcggcacg ctgatgtacg
    10381 cgcagaactt ctacatcgcg ttctacgacg cccgcaccga cagcgtccgg tttccgtatt
    10441 acgccgatac ggtcgatgcc gatccgccca agccggccac cgatttcccg atgaccgaga
    10501 tcctgcacgg tccgacctgg tacgtgatcc gtgatggcaa accgctgatg ggctcgctgc
    10561 cttcgatcgg ggcgcaggtg cacggcccgt ttcgcggcat cggcgccgaa tgcgtcgact
    10621 ggctgggcgt gccgttgttg cgcggcagcg aagtggccgg ctgcgtggtg gtgcagagct
    10681 acgacgatgc ccatcactac gacgaacagg acaaggcgct gctgatctac gtcgcccagc
    10741 acatccagac cgcgctggaa cggcggatgg cgcacgcgga gctggagcat cgcgtcggcg
    10801 agcgcaccga ggcgctgcgc gaagccaacc gcgtgctgca acagcaggtg ctggagcggc
    10861 agcgcgggga acgcctgcag gcggcgctgt tccggatcgc ggagcttgcc agcaccaccg
    10921 acagcatcga caacttctat gcggcggtcc accgggtggt cggcgggctg ctgtacgctc
    10981 gcaatttcta catcgcgctg ttgtccgaag acggcgccga gttgaccttt ccgtattccg
    11041 tggacgagcg cgatcgcaag atccagtcgc gcaagctcgc caacgggctg accgagtacg
    11101 tgttgcggca cggcaccgcg ctgctcgcca acgcgcccga gatcgcagag ctgcgcgcca
    11161 gcggcgaagt ggcgcaacaa gggccggatt cggtgtgctg gctgggcgtg ccgctggtgt
    11221 gcgccgacca cacggtgggc gcgctggcgg tgcaaagcta ttcgccggac catcgttaca
    11281 ccttgcgcga ccaggaactg ctgaccttcg tctcctacca catcgccaac gcgctggaac
    11341 gggtgcgggc gaccgaatca ctccggcacg cctacgccaa cctcgagcag cgggtcggcg
    11401 aacgcacccg cgcgctggcg ctcgccaacc gcgacctgcg cgcgcagatc gccgaacgcg
    11461 agcgcatcga agcgcgcctc aaatacgaaa cgctgcacga ttcgctcacc ggcctgccca
    11521 accgcagcct gctgatgcag cggctcgaac atgcgctcga acgcttccac gccgacccca
    11581 gcaaggcctt cgcggtgctg ttcatggacc tcgaccgctt caaggtcatc aacgattcgg
    11641 tggggcacct gatcggcgac gacctgctgt tccaggccgg gggacggatc cgcgcctgcg
    11701 tcaagtcgga agatgtcgtg gcgcgattgg gcggcgacga attcggcgtg ctgctggagg
    11761 gcgtctccga cgccagccag gcgtgccgca tcgccaagcg catcatcgac gacttgaacg
    11821 cgccgttccg gctcgcggcc aaggagcttt tcacctccac ctcgatcggc atcacgctcg
    11881 cgacgtcgca ctaccgccac cccgaggagt tgctccggga cgccgactcg gcgatgtacc
    11941 gcgccaaggc cgacggccgg caccgctacg ccgtgttcga cgaagggctg cggcgcgagg
    12001 ccgtgtccct gatggaggtc gagaacgacc tgcgccgcag cctgacgcgc ggcgagttcg
    12061 tgccttacta ccagccgatc gtggacctgg ccgacggttc ggtgctcggc tacgaagccc
    12121 tgatgcgctg gcgacatccc gagaacggac tgctgctgcc ggccgagttc ctcggcgtcg
    12181 ccgaggatac gggcgcgtcc gaaaccatag actggcagat ctacgaacag gtttgccgcg
    12241 atgccaattc gctggcggcc ggggatggcg tgttcgtcag catcaacctg tcgccgcggc
    12301 atttccgcaa tcccgaactg gaccggcggc tgctcaggct gctcgccgac catgacgtgt
    12361 cgcccgtgcg gatacgggtc gaggtcaccg aacgggcgat gctcgagaat ccgcccgaag
    12421 ccaagcggat cctgcagacg ctgcgcggcg ccgggatcgg catcgcgctg gacgatttcg
    12481 gcaccggcta ttcctcgctg tcgtatctgc accagtatcc ggtgcaggcg atcaagatcg
    12541 accgttcctt catcaccggg ctcgatgccg gtagcgaagg cggcgccgcg gtgatccgca
    12601 ccatcctcgc gatggcaaac ctgctgtcga tgcaggtgat cgccgagggc gtggaaacgg
    12661 ccgcgcaacg cgacctgctg atcaagatgg gctgccgggt cgcgcagggc ttcctgtact
    12721 cgtccgcgca accgctggag gcctgggtca gcgacacggt gcccgaagcc ccggcatgag
    12781 ccgagcggca gggacccgcg ccgccggtcg gcgctcgttc gcgcgccgct ggctgcgacg
    12841 gctgctgtgg cttctcgtgg cgcttgtcgc ggtcacctgg ctgatggtgc tggcgctgcg
    12901 tttcgtcccg ccacccacca gcgcctacat gctcgagcgc aaggtaagcg aaacgtggcg
    12961 cggcaaccac gacttcaggg tccgctatca ctgggtgccg ttcgagcgca tggcgccagt
    13021 ggcgccgctc gcgatggtcg cgggcgagga ccagacgttc cccttcaacc acggtttcga
    13081 catcgcctcg atccgctccg cgatcgacgt ggcgcaatcg ggtggccggc tgcgcggcgc
    13141 cagcacgatc acccagcaga ccgcccgcaa cctgttcctg tggtccggcg gcggcttttt
    13201 ccgcaagggg ctcgaagcct ggttcacggt gttgatcgac atcacctggc ccaagcgccg
    13261 cgtgctcgag gtgtatgcga acatcgccga gctgggcaac ggcatttacg gcgtcgaggc
    13321 cgcggcgcaa acgtatttcc acgagcccgc atcgcggctg acgaagcgtc aggccgcgct
    13381 gctggcggcg gtcctgccgg atccacgcga ctggcacgcc gcacgtcctt cggcctacgt
    13441 gcagcggcgc gcggcgtgga tcgagcagca gatgcggcag ttgggtggcg tgtcctacgt
    13501 gctcgacaag cacccgccga ggcccagggt caaagaggcg aagaaccggt tccgcaagcg
    13561 ctagactggt tgcggcacat cgcccgccac ccaaggagat tcccatgcga caggtcaaac
    13621 atctgttgga aggcaagggc aacgcggtct acacgatcac gccggaggcg ccggtgctcg
    13681 acgccatccg cctgatggcc gagcgcagtg tcggtgcgtt gccggtgatg cgcggcgagg
    13741 cgctggctgg catcgtcacc gagcgcgatt acgcgcgcaa agtgatcctg aagggccatt
    13801 catcgcggga aacgccggtc tcggacatca tgaccccggc gccgaccagc gtggcaccgg
    13861 tgaccaccgt cgatgaatgc atgcgtctgt gcaccgaact gcgtgtccgc cacctgccgg
    13921 tcctggagaa caacaagctg gtgggaatcg tatcgatcgg cgacctcgtc aaggcggtga
    13981 tcgacgacca ggccgccgag atagaccact tgcagcgcta catcgcgggg tgagccgggg
    14041 ctttcgtgag atgccagtgg catctcacgc cggcgaacgc ccgcacaggg aggtgcgggc
    14101 cggcttcacg caccatggat ggctgctcgc cgaaaacctg atcgcgcgac gtccgacatc
    14161 gcgaagtcgc ttcactggac ctgcgacacc atccacttga ccgtggcttc gacctgttcg
    14221 tcggtgagcg ccgggttgcc gcccttggcg ggcatgtggt tgccgtcagg cccggtgtag
    14281 ccgtcgatcg cgtgcttgac gagggtgtcg atgccttcct tgaggcgcgg cgcccacgcc
    14341 gccttgtcgg tgatcttggg cgcacccgcg gcacccgtcg tatggcacgt cgtgcaaagc
    14401 ttgtcgaaga ccaccttgcc gtcggtggtg ccgccgtagg cgacctgcga agccgcggcc
    14461 ttggtggcct cggccttggc ggcctgcatc gcggcgcggc cggtgtcgcc ggcgtatacc
    14521 gcgccgaccg gcgtgatccg gtcgcctgcc tgtttcacct gggcctcccg gccgtccttc
    14581 ggcagcgtcg accacaccag cgcgccgatg atgatcagga tgaccgccgt gcctccgagc
    14641 atcgcgatca tgatcgcgaa ttgtttcatg aaggtacggt cggacggggt gagatgcgtg
    14701 ctcactggca gactccggta aatggaccga cggcatcccc gggcccccgt ggatgccgca
    14761 tatccgcaaa agtatagccg cccggtggcg cggccaccaa atgtcgcgcc cattgacctg
    14821 atgtgcacgc cgcggccggt acaactcccc actttccgtg cgcggtagca tcgttacgcg
    14881 ggcatgcccg aacactacga catcatcgtg atcggttccg gtccgggcgg tgcgagcctt
    14941 gcgcaccggc tggcgccgtc cggtttgaag atcctgatcc tcgagcgcgg cgactggctg
    15001 aagcgcgccg ccgccaactg ggatccgcgc gaggtgttca tcaacaacat ctaccaggcg
    15061 gacgaaacct ggtacgacaa gtccggcgag gcgttccatc ccggcctgca ctatttcgtc
    15121 ggcggcaatt ccaagatgta cggcgccgcg ttgttccggt tgcgcgaacg cgacttcggg
    15181 gaactccggc acgccgacgg cctgtcgccg gaatggccgt tgaagtacga cgtgttcgag
    15241 ccctactacg acgaggccga aaggctgttc cacgtccacg gcgcggccgg cgaggatccc
    15301 accgagccgc cgcgcagcag gccctatgcc tggccgccgg tgtcgcatga accgcggatg
    15361 caggagctgt ccgacacctt cagccggcac cacctgcacc cgttccacat gccgctcggc
    15421 atcctgctgg aggagcagga cggccgcgtg ttgcgccgca gcccatgcat ccgctgcggc
    15481 gcgttcgatg gttatccgtg cctgaccaac ggcaaggccg atgccgaggt gatctgcatc
    15541 gatccgatgc tcggcgccca tgacaacgtg acgctgctga ccggcgcgta cgtctccagg
    15601 ctcgacaccg atgccggggg ccacgcggtg agcacggtgc aggtcgagcg tggcggcgcg
    15661 aaggaaagtt attcggcaaa cgtcgtggtg gtcgcctgcg gcgcgctctc gaccgcgctg
    15721 ctgctgctgc gctcagcaaa cgacaagcat cccgacgggc tggccaatgg ttccggccag
    15781 ctcggccgca actacatccg gcacaaccag tcggtgttga tggcgatcac ccgccaaccc
    15841 aacgacacgg tgttccagaa gaccgtgggc gtgagcgact tctacttcgg ggccgacgac
    15901 ttcgaattcc cgctcggcct gatccagatg accggcaagt cgcacccgga cacgatccgc
    15961 ggcgaagcgc tgccggcatg gctggagtgg cttccggaga agccgttcga atacctcgcc
    16021 aggcattcac tggatttctg gctgtcgagc gaggacctgc cgaggccgga aaaccgcatc
    16081 cgctacgagg gcgggagggt ggtggtcgaa caaaccgaca acaacatgca gggacaccgg
    16141 cggctgcggg cgaaactcga aagcctgctg ggaccgatgg acgcgcaccc cttcatgctc
    16201 gagcgcaact actacctcgg gaaggacgtc ccgatcggcg gcaccgcgca ccaggcgggc
    16261 accgcgcgct tcggcgaaga cccggcgaca tccgtgctcg atctcgactg caaggcccac
    16321 gaactcgaca acctgtacct cgccgatgcc agcttctttc cctccatcgg cgcagtgaac
    16381 cccaccctca ccatcatcgc caacgcgctg cgggtggcgg accgtatcgc ggagcggatg
    16441 aagtgaacgc ggtgtgacct cacgcgagga gccgtaccct cacccggcgc ttcgcgccac
    16501 cctctccgat gggagaggga caatccaacc gaggagcgcc ccatgtggct ggacctctac
    16561 gtcatcccga tcctgattcg cgtgtgcctg gtggtgctgt tcccgttcag cgcgctcgac
    16621 aagatcgtgt tctggaagga cgccctcgcg caggcggact cgtcgttcct gccgaagtcc
    16681 agcggaccgc ccttgctggc caccgccatc gtggtggaag ccattgcgcc gttctgcatc
    16741 gtcgtcggct ggcatgaccg gatcgcggca ctggtcctcg cgcttttttg cgtggtcacc
    16801 gcgatcctct accacccgtt ctggaagttc gccgatttct ggaagccggg cgacagcatt
    16861 gcacgcagcc acttctggga tttcctcaag aacttcgggc tggtcggcgg gctgttgctg
    16921 gtggtgttcg cgacacgact cgcgccggcg ggcgtgatcg cgcaccggcc gctttcctcg
    16981 accgcggtgt acacggcggc gggcaccgcg ccggcatccg ccgttccagg ccgcacgcac
    17041 gacatgcaaa ggtagggcga aaaccggggt cagagtgcac tttctcgttg cgacaccaag
    17101 cagcgcgatt gcttccacga aggtgcattc tgacccccgt tttccatcgg agaatcccat
    17161 ggcacacggc cattccaacg aagcacccac tctggcgtac tggcacgtgt gggtggacga
    17221 cgacggtgtc agcaggcagg agcgacgctc actcggccgc ttcgagcagc agagcatggg
    17281 cggcgcggcg ccgcaatgga acgcggtgca gcccgccgcg cccagcaagg cgattttcac
    17341 ggtgcttccg gtcggctgga gcggcgaatg gcacgagaat ccggcgccgc agtggatcgt
    17401 tccaatgagc ggtcgctggt tcgttgaaac gatggatggc catcgcgtgg aaatgggcgg
    17461 gggcgaactc tcgttcggcg gcgaccaggg ttgcaccgcc gacgccgaag gccgcaaggg
    17521 acaccgctcc ggcacggtcg gcgatgagcc cgcggtgctg atgctcgtcc agatggatga
    17581 cggatgttgc agacagtaaa caccaaagcc ttcgacgcgg ataagagcag aaaaagcgga
    17641 tagaaacccg atccgtgttt atccgcgtcc aatgcttctc cgccttcgtc ccggccttcc
    17701 caaaacaaca atgagcgatt acgaaatcct cgatccgcgt ttccgcgcaa tggtgctgcc
    17761 caatgcacct ctggagaaac tcgccgacgg ctttcgatgg ctggagggac cggtgtggtt
    17821 cgcggacctg cggtgcctgt tgttcagcga cattccgaac gaccgcatca tgcgctggac
    17881 ggcgagcggc ggggtgtcgg tattccgcga gccctcccga ttcgccaatg gtcacacgcg
    17941 cgaccgcgag ggacgcctga tcgggtgcct gcacggtggc cgcgccgtcg tgcgcaccga
    18001 gcacgacggc accgtcacca ccctcgccga ccgctaccag ggcaagcgcc tcaactcgcc
    18061 gaatgacgtg gtggtgaaat ccgacggcag catctggttt tccgatccgc cctacggcat
    18121 ccagaccgac tacgagggcg gccgccgcga atccgaactg ccggccaacc tttaccgcct
    18181 cgatcccgca aacggctcgc tccaccttgc gtgcggcgac atcgcgggcc ccaatggatt
    18241 gtgtttctcg cccgacgaaa agcgcttgta cgtcaccgac accggcatcc agttcgatcc
    18301 cgattcgccg cgctgcatcc tggcgttaga tgtcgccgaa gacggcaaga ctctctccga
    18361 tccacgcgat ttccacgcgg tttcgcccgg ccatgccgac ggcatccgct gcgacgagga
    18421 cggcaatgtg tgggcggccg ctggcgacgg cgtgcactgc attgcacgcg acggcacttt
    18481 gctcggcaag atcaaggttc cggcgacggt gtcgaacttc tgcttcggcg accgccacta
    18541 cagccgcctg ttcctgtgcg catcgcaggc gctgtacgcg atctgggtca accggcgggg
    18601 cgcccagcgg ccgtgaggca cggcgtgcaa cgcctgctgc gcatcgagcg caacacgcgt
    18661 gacctcgagc gcgcgatcgc attccaccgc gacgcgctgg gcttctcggt gatcgacgcg
    18721 gatgcgtcac cgccggcctg gacgcagctc cccggcctgg atgcggcacc ttcgcggtgc
    18781 gcgcggcttg ggctcggcga gcaggaaatc gagttcactg aattcccgga cgccgcgccg
    18841 tatccgcacg gcagcacctc ctgcgatatc gggttccagc actgcgcgat cgtcgtgtcc
    18901 gacatggagg ccgcgtaccg gcggctcgtc gtcgaaggca cggttccgat cacgcagggt
    18961 ggtccgcagc aacttccgcc tgccaccgga tcggtcacgg cattgaagtt ccgcgacccg
    19021 gatggccacc cgctggaact gatctggttt ccggaaggca ccggcgaccc gtgctggaaa
    19081 aagccgcgca gcgggaatcc ggcgacgctc ggcatcgacc acaccgcgat cagcgtcgcc
    19141 gacaccggcc gcagcgtgct gttccacgaa ctcgtcggcc tgcacgtggg ggcacgcggc
    19201 gtgaaccgcg gcattgaaca acagcggctc gacgacctgg ccggtgtcga agtcgacgta
    19261 atcgccatgc aggcggccgc ccatacgccc cacctggaac tgctgggcta ccgcaccccg
    19321 cgcggtcgcg tggcgccgcc cgcgcccatc accgccgtgg tggcagaccg cgtggtctgg
    19381 caggcggaag ccgtggacgt gctgctcgag gcggccaccg atggcggttt cgccgacggg
    19441 gtggtcgcga gcggccgcgt ccgtggcacg accatcgcgc tgctgcacga tcccgacggc
    19501 caccggatgg tcctggccgg cgcggctgcc tgaacgcccc ggcaacgccg ggttcgctac
    19561 catcgaagac tccgcccgcg gagtacctcc atccggtgcc acacagtcat cgccccgaag
    19621 catggatccg cagattcagg atgcggcgcg ctgcccgccg tgtcgcgcac gcccatgccc
    19681 gggccttgcc gtacgaggca cccgacgacg aagccccacc cactcctgcc aacatcctcg
    19741 cgcggcagca cggccagctc accagcgccg cgccgttcga tctcggcagg gcgctggcgg
    19801 cgtacgacca ggccctcgcg cgggcctatt cggtcgcacg caccgccgcc accgacaatc
    19861 gccgcgtcga gccggccgcc gaatggctga tcgacaacgt ctacctggtc cgcaacgaga
    19921 tccgcgaagt ccgcgaagcg ctgcccgcca aggtatggcg gcggctgccg cggcaggcgg
    19981 tcgagggcgg cgtggtcgtt ccccgcatgt tgcgggtact gcgtgcatcc atcgcgcagc
    20041 tcgacggcaa catcgaaccc gacgccatcg aagactacct cgaggcatac cagcggcgcg
    20101 cttcgctcga cctcgtcgag ttgtggacgc tgccggtgct cgcgcgggtc gcgctgatcg
    20161 agagcctcgc cgccggcgcg aacacgatcg cccggcgtct cgaggcctac tcgaacgcgg
    20221 agttctgggc cgaacacctg atcacggtgg cggcacgcgc gcccaacgac atgctgggca
    20281 gcgtcgcgga gatggccaga gcgaacctgt tcgtctcgcc cgcgttcgcg gcggagttct
    20341 accggttgct ggaaggcaag cacccgtcgc tgaaactcgc gctggggctc gccgaacagc
    20401 aactcgagca acaaggcacc agcgtcgcgc gggtgatcga cagcgagagc cgcgcacagg
    20461 ccgccgacca ggtgtcgatc gccaatcgca tcaacagcct gcggcggatc accgactaca
    20521 actggtcgga cctgatcgag cgggtcggga cggtcgaccg gatcctggcg ggcgaccccg
    20581 ccggcgcgta catgcggatg gacttcacga cccgcgggcg ctaccgccac gcggtggaat
    20641 cgctggccca gcgcagcggc cgcggcgaac ccgacatcgc gcggcgcgcg gtcgagctgg
    20701 cgggcgcgga gcgcgccccc ggttcggatc cgcgcactcg tcacgtgggt tacttcctcg
    20761 tcggcgccgg ccgtgacgcg ttcgcacgcg agctcggcgt gcggcggtcc gccgaatacc
    20821 ggatcgcggc acggctcagg cgcttgccgt cgacaaccta cctcggcagc atcttcatca
    20881 ccaccgcgat catcacggcg gtggtcgcct cggtgcacca ctggtacctc gctccgctgc
    20941 cgatcgccgc cgacaccgcg ctgctggtgg cggtgttcgt cgccgccagc cagccggcag
    21001 tgaccctggc gaattgggtg ctcgggatgc tggtgcccgc gcaactgttg ccgcggatgt
    21061 ccttcagcgg cggcatcccc gatgagtgcc ggacgctcgt ggtggtgcct tggctgttgg
    21121 gcagcaccag cacgcttgcc gaacaactcg acgcactgga aatccgctac ctcggcaacc
    21181 gcgacccgaa cctccgctac gcgctgctga cggattttcc cgatgcgccg gagcgcgaca
    21241 tggccggcga cgccacgctg ctcgcggcag ccagcgccgg gatcgagcgg ctcaacgcga
    21301 agcacgcgtt gccgggcagc acccgtttct atctgttcca ccgcccgcgc gaatggaacg
    21361 cgcggcagtc cgcgtggatg ggcttcgaac gcaagcgggg caagctcggg gacctgaatc
    21421 gcgtgctgcg cggtgcacgg cccgcccgtt tcttcagcaa ggtggtgggc gacctcgcgg
    21481 cgctgcaggg cacccgctac gtgatcacgc tggacgccga cacccagttg ccgcccgagg
    21541 ccgcgcgcaa gctggtcgaa accatggccc acccgctgaa ccggccctgg ttcagcaagg
    21601 ggtcgcgccg cgtggtcgcc ggctacgggc tgctgcagcc gaggatctcg gtgacctacg
    21661 gcgccgaggg ccccagccgc ttcgcgcggc tgtacagcga tgcgacgggt ctcgatccct
    21721 acacccgcgc ggtatcggac gtgtaccagg acctgttcgg cgaggcctcg ttcgtcggca
    21781 agggcgtcta cgacgtcgac gccttcagcc gttcggtcgg cacgcgtttt ccgaatgacc
    21841 tgatcctgtc gcacgacctg ctggaaggca gctacgcgcg cgccggcctg gtcagcgaca
    21901 tcgagctgat cgaacaccag ccaaaccgct tcagcgtgga cgtgcgccgc cgccaccgct
    21961 ggacccgcgg cgactggcag atcgtgcaat ggctgctgcc gatcgtgccg ggcccgcgcc
    22021 ggcgtggcct gcgcaacacc ctgaaggccc accatcgctg gaagatcctg gacaacctgc
    22081 gccgcggcgt ggtgcccttg gcgatgctgg cgctgttgct gggcggctgg aaccacagca
    22141 accacggcgc ctactggagc ctggtggtgg tcgcgttcct gctggggccg ccgctgctga
    22201 tctcggccgt ccaggtgctg cgcaatttgt ggcaacgggt gccaccgcgg cgctggcgca
    22261 acacctggcg caacatccgc cacgaactgt tgcgcgacgt attcgcgatc gcggtgctgc
    22321 cgttcgagac ctggctcaac ctggatgcca tcctgctcag cggctggcgg ctgatcttca
    22381 cccgcaggca cctgctggag tggacgccga tgaccgacgc cgaacgcggc gcggcgtcgg
    22441 gcctcccggg ctacacgcga ctgatgtgga tggcgccatc ggtcgccgtg atcgccggct
    22501 tcgcgctcgc gttcgagaac ccggtcgtgc tgccgccggc gctgccgttc ctcgtgttgt
    22561 ggttcgtcgc gcccttgctc gcgtggtgga tcagccgcac cccgcaacat ccggcgctgc
    22621 cgctggacgc caggcagcgc ctgttcctgc acgcggtggc gcgccgcgtc tggcactggt
    22681 tcgacacctt cgtgaacgag gacgagcact ggctgccgcc cgacaacgtc caggtcttcc
    22741 cggtcgaaca ggtcgcgcga cgcacctcgc cgaccaacat cgggatggca ctgctggcca
    22801 ccctgtcggc ttgcgatttc ggatacatca cccgcgacgg cttgttgcgc cgcaccggcg
    22861 cgaccctcga aacgctcggc aagctccagc gccaccgcgg ccacttctac aactggtacg
    22921 gcaccgcgac gctggagccg ctgtcgccga tgtacgtgtc gagcgttgac tcgggcaacc
    22981 tcatgggctg cctgctggtg ctcgccaacg ccctccagca caccgacagc gatccgctgg
    23041 tgccgccgaa tctcgccagc ggcctgcgtg acaccgccca actcctgggc gaactgctcc
    23101 ccaccatgga gccgacggcg ccgcacgcca tgctggcctt gtcggcgacg ctcgccgaac
    23161 tcgacgcact gctgcaggac gcgatcgcca acgccagcgt gctgccggtc ctgcatggat
    23221 tgctggcgcg gatcgaaagc gagtccggca agctcggcca gatcgtcaac gacaacgcca
    23281 tcggcggcga attcgcggaa tgggtggggg tgctggctcg gcaggcgacc gccgcctgcg
    23341 aggaactcga cgagctggcg ccgtggctcg ggctcggcga ctcgggcagc gacgacgaac
    23401 aggcacgcgc gtggctggtg gagctcgagc acaaccccga caccgcccgc gcccggacgc
    23461 tcgccgccgg cgtcgtcggg cggctggaag ccgaaggcgc tgccgattcc tgcgccgcgc
    23521 tgcgcgaact gcaggcgacc ctgaaccggc gccgcgatga cgcgctggcc ctggccgcgc
    23581 aatgccggga attcgcggcc atggacctct cgttcctgtg gaacgaacgg cgcaggcaat
    23641 tcacgatcgg ctacgacgtc gcgcgcgaca agcgcgacgc cagccactac gacctgcttg
    23701 cgtccgaagc ccggatcctt tcctacgtcg cgatcgcgca aggacaggtg ccgccgaccc
    23761 attggttcca gctcggccgc ctgctcacgg tggccgcggg ccggccgacg ctggtgtcgt
    23821 ggagcggctc gatgttcgag tacctgatgc cgctgctgct gatgcccaac taccgccaca
    23881 ccctgatcgc cgacacctgc ggcgcggcgg tcgcgcggca gatccagtac ggcgcgcaac
    23941 aacaggtgcc ctggggcgtt tccgaatccg cctacaacgc gaccgacgcc aacctcgtct
    24001 accagtaccg cgcgttcggg gtgccggggc tgggcctgaa gcgcggcctc ggcgaggacc
    24061 tggtgatcgc gccgtacgcc acgacgatgg cgctgatgat cgatccggcc gcggcctgcg
    24121 ccaacctcca gcggctcgcg gaggtcggcg cactgacccg ctacggcttc tgggaggcgc
    24181 tcgactacac cgcaggacgg gtcagcgaga acgaggactt cgcactggtg aagagctgga
    24241 tggcccatca ccacggcatg agcctgctcg cgatgtcctc gctgctgaac cgccagccga
    24301 tgcagcagcg cttcatgcgc gaaccgatgt tccgcgccta cgagctcttg ctgcgcgaga
    24361 agatcccgga ggcacgcccc cggcatccgg cgacgctcga cgtcgagcag ccgcgccaga
    24421 cccgcgcccg cgtccacgcg cagacgcgcg aaatcctgga catggacacg ccgctcccgg
    24481 aagtgcacct gctctccaac ggcaactacc acgtgatgct gacccaggcc ggggcgggct
    24541 acagccgctg gcgcgaactg gcgctgaccc gctggcacgg cgacgcggcg agcgacgacg
    24601 agggcctgtt cgtgtacctg cgcgacgtcg actccgggca ggtgtggtcg gcgacgcgcc
    24661 agcccatcgg ggacgccggc ggcacctgca gcgccacgtt ttcgcaggcg cgcgccgaat
    24721 tccaccgcaa cgatttcggc atcgccaccg aaatggtggt cgcggtgtcg gccgaggacg
    24781 acatcgagct gcgccgcctg cgcctgacca accgcacgct gaagccgcgc acactcgaaa
    24841 tcaccagcta tgcggagatc gtgctggcgc cagccacggc cgatgcggca cacccggtat
    24901 tcagcaagct gttcctcgag accgaatcgg cgccggagct ggatgcgctg ctggcgacgc
    24961 gccgcgcgcg cggccccgac gaccaccatc cgtggatgct gcaccagata accacccgcg
    25021 gcggcacgat cggcgaaggc gcgttcgaga cggaccgcga ggctttcctc ggccggctgc
    25081 acggtccggc ccatccgcag gcgattgcgg gcgacgttcc gctggcgggc catgcgggtg
    25141 cggtactcga tcccatcttc tcgttgcgcc gccgcatccg gatcctgccg ggccacaccg
    25201 ccggcgtgga cgtcctcacc ggggtggcgc ccgaccgcga tgccgcgctc gcgctggcgc
    25261 gcaaatacca cgaacggcac ctgcaccagc gcgtgttcca gctcgcatgg acccacgaac
    25321 aggtggcgtt gcagcaactc aacatgacgg cggtcgaggc acaacgctac gagcggatcg
    25381 cgagcgcgct gctgtacgga ccgatcgcgg cgcgccgcga caccggcggc gcgctccccg
    25441 cgcgttcggc gctgtggaag cacggcatct cgggtgacct gccgatcgtg ctggcgcgga
    25501 tcgccaatgc cagccagatc gacctcgtac ggcatctgat ccaggcccac ggctactggc
    25561 agatgcgcgg cctgcacgcg gacctcctga tctggaacga ggaactgtcg ggctaccggc
    25621 aggagctgca ggagcacatc ctttatttga tcgagtccag cgccgaggcg cgcggggggc
    25681 tcgggcgcgg gcggatgttc gcctggcgca tcgagcacct ggcggccccc gatcgcgcat
    25741 tgatgctggc catggcgcgc tgcgtgtttt cgggcgacgc cggacgcctg cgcgaccaga
    25801 tcacgcgcct gctgccgccg ggggaaccgt ccaggccggt ggcgcgcacg ccgcggcaca
    25861 aaccgcaagc cggcagctac gcgctgcaac tgccttcgga ggaccttcgc ctttccaacg
    25921 gattcggcgg cttccgcaac gacggcagcg agtactcgat ctggctcgac ggggacgcgg
    25981 cgacacccgc gccctggtgc aacgtgctcg cgaacgccga cttcggcacg gtgctcagcg
    26041 aaaccggcgg cgcctacacc tggaaggaga acgcccacga ataccgcctg agtccctggt
    26101 acaacgatcc attgcgcgac ctgagcggcg aagccgcatg gctgcgcgac gaagccaccg
    26161 gcgaatactg gtcgttgacg ccgcaaccga cccccgacgg cgacccccac cgtgtgtgcc
    26221 atggcttcgg gtcgagccgc ttcgagcacg tgcacgcgga cctctacagc gaatggaccg
    26281 tctgcgttgc cgcggaggcg ccggtcaagc tgtcgctgct gaagctcgtc aacaagagcg
    26341 accgggtccg ggtgatatcg ctgaccagct acgtcgagtg gaccctgacc gaaaaccgcg
    26401 cgcgcagcac gggccacgtg cggacctgga tcgaccgggg ctctggcgcg ctgttcgcgc
    26461 gcaatccgtt cgaagacacg tttgccgacc gcgtcgcgtt ccatgcattg gccgcgcggc
    26521 cggtgacgca ttgcgccgac cgccgcgcct tcatcggccg caaccgcggc ctcgccgcgc
    26581 ccgcggcgat gcaccaggcc ctcgacggca cggtgggtgc gggcctcgat gcgtgttcgg
    26641 ggcagcgcac cgtgttcgaa ctcgctccgg gcgcggaaat cgaaaccgtg gtgctgctcg
    26701 gcgctgccgc gggcgaacac gaggcgcgcg aactggtgcg cgccttcgcg gacgtcgacg
    26761 ccgcgtacgc cgagctcgag cgggtgcgcg gcgactggca ccacgcggtc tcgcaactgc
    26821 acgtgcacac ccccgacgcc gccaccgacc tgatggtgaa cggctggctg gtctaccagg
    26881 tactggcctc gcggatcttc gcccgcagcg gctactacca gtcaggtggc gcatggggct
    26941 tccgcgacca gttgcaggac gtgatggcgg tgatgcatac gcgccccgac ctcgcgcgcg
    27001 agcaattgct gcgctgtgcg cgacaccagt tccgcgaggg cgacgtgcag cactggtggc
    27061 acccgcccgg cggcaagggt gtgcgcaccc gcatttccga cgacctgttg tggttgccgt
    27121 ggtgcaccgc cgaatacgtg cgcgtcacgg gtgacgggac ggtgctcgac gagaccgccc
    27181 cgttcatcga gggccgtcgg ctggcggccg acgaggagtc cgccttcgag gacgtgcacg
    27241 tcaccgacga agaggccagc ctgtacgaac actgcctgcg ggcgatccgc gcgagcctga
    27301 agtccggcag ccacggcctg ccactgatcg gcggcggcga ttggaatgac ggcatgaacc
    27361 ggctgggcct cgaaggccgc ggcgaaagcg tgtggctcgg catgttcctg cacgacgtgc
    27421 tgacacgctt cgcgcccctg gccgaggcgc gcggcgatgc cggcagcgtc cgtcaattcg
    27481 gggcagccgc cgccgaactc gcggcgatgc tcgagcacaa cgcgtgggac ggcgactggt
    27541 acctgcgcgc ctggaacgac gacggcatcg cgatcggatc cagccaaagc gaagaatgcc
    27601 ggatcgattc cctgccccag agctggtcgg tgctggcggg catcggtgac gcggcgcggc
    27661 gcgagcgcgc gatggacgcg gtgctggagc ggctcgtcga cgaggaggcc ggcatcgtga
    27721 aactgttcgc gccgcccttc gacaaggccc cgctcgatcc cggctacatc aagggctacg
    27781 tgccgggcgt ccgcgaaaac ggtggccagt acacccacgc cgcgatatgg gtggcgatgg
    27841 cgctggcgaa gctcggccgg gcggacgatg cctggcggat cgcgcgaatg ctgaacccga
    27901 tccgccacag cgaggcgacc gaggcggcgc ggcgctaccg gctggaaccg tacgtggtcg
    27961 ccgccgacgt gtactcggca cccggccacc tcggccgcgg tggctggagc tggtacacgg
    28021 gatcggccgg ctggatgtac cggctgctca ccgaatcgct gctcggcctg cgccgcgaag
    28081 gcgaacggct gacgttcgcg ccgctggtgc cggatgattg gcggcgctgg tccgccgact
    28141 ggcgccacgg aggcacgcgc taccacatcg aattcgtgcg ccagccaggc gacaccggcg
    28201 tcgccgacgt cagcgtggac ggtgccttgc agtccgggca cgccatcgac ctggtcgacg
    28261 acggcgacaa ccatgaagtg ctggtgcggt tcgcgaagat ggaaggcgcg cgtgcgaccg
    28321 aagacagcga gcagcgcgcg acgtccaggg acgccagtgg
//
LOCUS       DASSNH010000094         7368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1776176, whole genome shotgun sequence.
ACCESSION   DASSNH010000094 DASSNH010000000
VERSION     DASSNH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776176"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..2023
                     /locus_tag="VFK00_03315"
     CDS             38..2023
                     /locus_tag="VFK00_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HET7592015.1"
                     /translation="MFKNRAGLRSRVLTLAVAGALGVAGTAVAAAPAGGQFSVLTKAT
                     QLGRGNTVQSAVPLNKSINISVVLKLRNGDQLKSFLSKAGPGRAMGPAQLQSHLPTGA
                     QAQAVAAFLKRAGFSNVEISSNRMIVNATGNAAAIKSAFQTSLVSVRTRDGRTAYANN
                     SAIRIPAALQGSVQAVLGLQTVHKMKTMPRVLEKPGVFGHLPTEFPDIYNASSLPAAT
                     DVDVAVWGWGGMQETVDDFNDFMSVSGLDSGPVTVVCTDYNGYDAGGVVTNDPTCNNF
                     DQGSSEWDMDSQSILGMTGGTKSMTFYAAYGGYNFSIVNALNEIVTPTVGEPLAQVVN
                     ASFGECERYQDANQGGDGSMQAMNALFQVGAAQGQTFSISTGDSGADECGDGQKDSAS
                     FPASSPWVVAVAGTTLRATTTTWARENVWGGSGGSPSSANEAQPWQAPLTYGAFAGQR
                     GPDVAFDGNPSSGELVLIGGQYYQIGGTSLSAPLFAGAWARILQGNPDLGFAAPHLYT
                     LPASAIHDVRSGNNHGWLAKRGWDWASGLGSLDVSAAAQALTGGGGGGDCTSGLQNGV
                     PVTGLSADEDGQLNFCVEIPAGASNLVVTTTGSNGDADLYVSFGTPPTLSTYDCRDYN
                     VGSNGTCSFSSPQAGTYYIMINAYQAYTGLTLEATWQ"
     gene            complement(2232..2684)
                     /locus_tag="VFK00_03320"
     CDS             complement(2232..2684)
                     /locus_tag="VFK00_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592016.1"
                     /translation="MGLCDERLHSGLIAEFQDDGNLDGFVHCHGACHGVAAMKLRCLA
                     EHAEVAGGGHGRTCNTKRRDNGRRKNPATQSCTILEHGLFLHGNVKGHRTRGRCIARC
                     YAINNIGKSSIGPGHLRFRPARATITTNGLHAMVPASPSDRRASHARS"
     gene            2604..4106
                     /locus_tag="VFK00_03325"
     CDS             2604..4106
                     /locus_tag="VFK00_03325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HET7592017.1"
                     /translation="MNKPIQVSVVLKLRDEAGMKAFIAQSHPSGSRHVMSPAQLQNHL
                     PTHAQAAAVADFLRGAGFGNVRISSNRMIVNAIGDAASVQSAFQTSLVNVRTHDGRTA
                     FANSSAIRIPATLKGSVQAVLGLQTVHTAHTMAQPNAGLGRFITGHYPVEFADIYGAS
                     SLSTATDVDVAVWGWGGMQETVDDLDYFMSTTGLSAGPVTVVCTDYNGYNAGGVVSND
                     PTCNNVDQGSIEWDMDSQSILGMTGGVKSLTFYAAYGGYNGSVTNSLNEIVTPTVGEP
                     LAQVVNASFGECERFQDSAQGGDGSMQAMNALFQVATAQGQTFSVSTGDHGSDECGDG
                     GEDSASFPSSSPWVVAASGTTLLATKKRYLLETVWGGSGGSPSSANLAQPWQAPLTYG
                     TYAGQRGPDIAFDGNPNSGEIVYVSAYGGYVQVGGTSLSAPLFAGAWARILQGNPTLG
                     FAAPHLYALPASVFHDVKLGSNGYSAQAGWDWATGRGSFNVGAAAAALQP"
     gene            complement(4200..5024)
                     /locus_tag="VFK00_03330"
     CDS             complement(4200..5024)
                     /locus_tag="VFK00_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7592018.1"
                     /translation="MEMNHIVFIVMDSCRYDTLMMADTPNIRRLGTPQKRYSYASWTA
                     PSHYTFTMGMMPHSSPREVLASEVYKDEYQQWTERIGGGGMEFKNFLPELSLPKVLSK
                     LGYCTVAKVSMPVLNKHTLISQHFDDYKLMGNHNDFAGMVEEMEFPDDQPQYYFLNLG
                     ETHYPYMLDGKDMPHVSGVHGALKSMGNGMPGAPVQVSDKSVLERLMGLQKQQVKCAE
                     YIDGLIGRLMKMAPRNTHFIITADHGEMFGEDGYFGHGPIMHEKCFEVPFLEGRLR"
     gene            5854..>7368
                     /locus_tag="VFK00_03335"
     CDS             5854..>7368
                     /locus_tag="VFK00_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592019.1"
                     /translation="MTGFVTFALAPAGYRDCAIAIAACRAGAVGIVNGELNPDRSALA
                     RELDEVSRRAKGPYGIKLDAMDAALEGALRAHAAAGLRWVVLDCALVAAVPGLVSDLR
                     GQGVRVLGEVTTPDWPGQPLDGVLDGLWIKGNESGGFVGEEASFILIQKWLKRTHLPL
                     YVRGGVTPHSAAACAAIGIAGGVLDSQLLLFDDLGLFETLNPLLRSLSGSETVAVGDG
                     ERGEYFRVLVRPGNPAAREFVAAGESECAEGLRPLLAGRTDWTRPTRSLLPIGQDVAF
                     AADWRVRYGSLHALVAAIDDAVADHLRAAVACHPIRENGPLAQALGIRVPVVQGPMTR
                     VSDTAEFALSVADGGALPMVALALMKEAALDSLLARTAELLEDRPWGIGLLGFAPQSL
                     LDEQVAVALKYKPDYAIIAGGRPDQAVRLDQAGIPSFLHVPSARLIPMFMQEGARRFI
                     FEGRECGGHIGPLSSFVLWSAMVDALLAELSGGKYEPSELQLLFAGGIHDAVSSA"
BASE COUNT         1220 a         2319 c         2519 g         1310 t
ORIGIN      
        1 tcgatgccct tcgaaacccc gtggagagga acaactcatg ttcaaaaatc gtgcaggttt
       61 gcgtagccgg gttcttacac ttgccgttgc gggcgcattg ggcgtcgcgg gcacggcagt
      121 tgccgccgca ccggccggcg ggcaattcag cgtcctcacc aaggcgacgc agctcggccg
      181 tggcaacacc gtgcagagcg ccgttccatt gaacaagtcc atcaacatct cggtggtcct
      241 gaaactgcgc aacggcgatc agttgaagtc gttcctgagc aaggccggcc ctggccgcgc
      301 gatgggcccc gcgcagttgc agagccacct gccgaccggc gcccaggcgc aggcggttgc
      361 cgctttcctg aagcgcgcag ggttcagcaa cgtcgagatc tcttcgaacc ggatgatcgt
      421 caatgccacc ggcaacgcgg cggcgatcaa gtccgcgttc cagaccagcc ttgtttccgt
      481 ccgtacccgt gacggccgca cggcctacgc caacaattcg gcgatccgga tcccggcggc
      541 cctgcagggg agcgtgcagg cggtactcgg cctgcagaca gtccacaaga tgaagacgat
      601 gccgcgggta ctcgagaagc ccggcgtctt cggccatctc ccgacggaat tcccggacat
      661 ctacaacgcc tcgagcctgc cggcggcgac cgacgtggac gtggcggtct ggggttgggg
      721 cggcatgcag gagacggtcg acgacttcaa cgacttcatg tctgtcagcg gcctcgactc
      781 gggtccggtg acggtggttt gcaccgacta caacggctat gacgccggtg gcgtggtcac
      841 caacgatccc acctgcaaca acttcgacca gggttcgtcg gagtgggaca tggacagcca
      901 gagcatcctc gggatgaccg gcggcaccaa gagcatgacc ttctacgccg cgtacggcgg
      961 ctacaacttt tcgatcgtca atgcactcaa cgagatcgtg acgccgaccg tgggtgaacc
     1021 gctggcgcag gtggtcaatg cctcgttcgg cgagtgcgag cgctaccagg atgcgaacca
     1081 gggcggtgac ggttcgatgc aggcgatgaa cgccctgttc caggtgggtg cggcgcaagg
     1141 tcagaccttc agcatctcga ccggcgactc gggtgccgac gaatgcgggg acgggcagaa
     1201 ggattccgcc agcttcccgg ccagctcgcc gtgggtggtt gccgtggccg gcacgacgct
     1261 gcgcgccacg acgacgacct gggcccgtga gaacgtgtgg ggcggttcgg gtggaagccc
     1321 gagttcggcc aacgaggcac agccttggca ggccccgctg acgtacggtg cgtttgcggg
     1381 acagcggggt ccggacgtgg cattcgatgg caacccgagt tcgggcgaac tggtgctgat
     1441 cggcggccag tactaccaga tcggcggcac cagtctctcg gcgccgttgt tcgcgggtgc
     1501 ctgggcgcgg atcctccagg gcaaccccga cctcggcttc gcggcgccgc acctgtacac
     1561 gttgccggcg agcgcgatcc acgacgtacg ctccggcaac aaccacggtt ggctggcaaa
     1621 gcgtggttgg gactgggcct ccggcctcgg cagcctcgac gtctcggcag ccgcccaggc
     1681 tttgaccggt ggcggtggcg gcggcgattg cacgagcgga ctgcagaacg gcgtgccggt
     1741 caccggcctc tcggctgacg aggacgggca gttgaatttc tgcgtggaga tcccggccgg
     1801 cgcgagcaac ctggtcgtca cgaccacggg cagcaatggc gatgccgatc tgtacgtgag
     1861 cttcgggacg ccgcccacgc tgtccaccta cgactgccgg gactacaacg tcggcagcaa
     1921 cggcacctgc agcttctcca gtccgcaagc tggtacgtac tacatcatga tcaacgccta
     1981 ccaggcatac accgggctca cgctcgaagc gacatggcag tgacgaaagg tgcgttgagc
     2041 acatgaggtg caacggaagc ccggcgcaat gccgggcttc ttctcggagg gaggaggcga
     2101 gacgggacca tccggacgcg tgacggcggc gtccatatgg gatttcgtgg tggtcggcgg
     2161 gcagcacgcc aggaacgcag gtttccgttt ccccgctgcg tcgtggcagc ccgagggcgg
     2221 tcgccgactt ttcacgaacg cgcatgcgag gcccggcggt cgctcggcga ggccggcacc
     2281 atggcatgca ggccgttcgt ggtgatggtg gcgcgggccg gccggaagcg gaggtgaccg
     2341 ggtccgatgg acgatttgcc gatgttgttg atggcgtagc agcgagcgat gcatcgacct
     2401 cgagttcgat gccccttgac attcccgtgg aggaacaacc catgttcaag aatcgtgcag
     2461 ctttgcgtag ccggattctt tcggcggccg ttgtcgcggc gcttggtgtt gcaggtacgg
     2521 ccgtggccgc ccccggcaac ttcagcatgc tcagcaaggc aacgcagctt catcgcggcg
     2581 acaccgtgac aagcgccgtg gcagtgaaca aacccatcca ggtttccgtc gtcctgaaac
     2641 tccgcgatga ggccggaatg aaggcgttca tcgcacagtc ccatccttcc ggatcgcggc
     2701 acgtaatgag cccagcccaa ttgcagaacc acctgccgac gcatgcccag gcagcggccg
     2761 tggccgactt cctgcgcggc gcgggcttcg gcaacgtgcg gatttcctcc aaccgcatga
     2821 tcgtgaatgc aatcggcgat gccgcgtccg tgcaatcggc gttccagaca tctctggtga
     2881 acgtgcgcac gcacgacgga cggaccgcat tcgccaacag ttcggcgatc cgcattccgg
     2941 cgacactgaa gggatcggtt caggcggtgc ttggcctgca gaccgtgcac acggcgcaca
     3001 cgatggcgca accgaacgcg ggcctgggcc gcttcatcac cgggcattat ccggtggagt
     3061 tcgcggacat ctacggcgct tcgagcctgt ccaccgcgac cgatgtcgac gtggcggtgt
     3121 ggggctgggg cggaatgcag gagaccgtcg acgacctcga ctacttcatg tccaccaccg
     3181 gactgagtgc cggtccggtc accgtggttt gcaccgacta caacggctac aacgccggtg
     3241 gcgtggtcag caacgatccg acctgcaaca acgtcgacca gggttcgatc gagtgggaca
     3301 tggacagcca gagcatcctc ggcatgacgg gaggcgtgaa gagcctgacg ttctatgccg
     3361 cgtacggcgg ctacaacggt tcggtcacca attcgctcaa cgaaatcgtc acgccgaccg
     3421 tcggcgagcc gctggcgcag gtggtcaacg cttcgttcgg cgagtgcgag cgtttccagg
     3481 acagcgccca gggcggcgac ggttcgatgc aggccatgaa tgccctgttc caggtcgcga
     3541 cggcccaggg acaaaccttc agcgtctcga ccggcgacca cggttcggat gaatgcggag
     3601 atggcgggga ggactcggcc agcttcccgt ccagctcgcc atgggtggtc gcagccagtg
     3661 gtaccacgct gttggcgacc aagaagcggt atcttctcga gaccgtgtgg ggtggctcgg
     3721 gcggcagccc cagctccgcc aacctcgcac agccctggca ggcgccgctg acttacggca
     3781 cctacgccgg tcaacgcggc ccggacattg cgttcgacgg caacccgaat tccggcgaga
     3841 tcgtctatgt gtcggcttac ggtggttacg tgcaggtggg tggcaccagc ctgtcggccc
     3901 ccctgttcgc gggcgcatgg gcgcgcatcc tgcagggcaa tccgaccctc gggtttgcgg
     3961 ccccgcacct gtacgcgttg ccggccagcg tgttccacga cgtgaagctc ggcagcaatg
     4021 gctacagcgc ccaggcgggc tgggactggg ccaccggccg cggcagcttc aacgtcggcg
     4081 cggccgcggc ggcgctgcag ccgtagtcca gatctgttcg accgcaacaa ccgcaccggg
     4141 gattctgcgc ccggtgcggt ttttctttga gcactagtcg gttgctcgcc ggggcagctt
     4201 cagcgaagac ggccttccag gaacggtacc tcgaaacact tctcgtgcat gatcgggcca
     4261 tgcccgaaat agccatcctc gccgaacatt tcgccgtggt cggcggtgat gatgaaatgc
     4321 gtgttgcggg gcgccatctt catcagcctg ccgatcagtc cgtcgatgta ttcggcgcac
     4381 ttcacctgct gcttctgcag ccccatcagc cgttccagta cggacttgtc gctgacctgg
     4441 accggggcac ccggcatgcc gttgcccatg gatttcagcg caccgtgcac gccggagaca
     4501 tgcggcatgt ccttgccgtc cagcatgtac gggtaatgcg tttcacccag gttgaggaag
     4561 tagtactgcg gctggtcgtc agggaattcc atttcctcga ccatgccggc aaagtcgttg
     4621 tggttcccca tcagcttgta gtcgtcgaaa tgctggctga tcagggtgtg cttgttcagt
     4681 accggcatcg acaccttggc gaccgtgcag tagccgagct tgctgaggac cttcggcagt
     4741 gaaagctcgg gcaggaagtt cttgaactcc atgccgcctc ccccgatgcg ctccgtccat
     4801 tgctggtatt cgtccttgta gacctcggag gcgagcacct cgcgcgggct cgagtgcggc
     4861 atcatgccca tcgtgaaggt gtagtgcgag ggcgcggtcc aggacgcgta ggaatagcgt
     4921 ttctggggcg tgccgaggcg gcggatgttg ggcgtgtcgg ccatcatcaa cgtgtcgtat
     4981 cggcagctgt ccatcacgat gaaaacgatg tggttcattt ccatgtcggc ctcccgtacg
     5041 cggccatggt gaggggtggt tctgtattgg tgcgatcagt cgcgatcggc aggagggggg
     5101 agcagcgctt cgttcggcgg gttcgacggg gagtgccggc tgctccggcg ctccatgcgc
     5161 ggcgtcgatg ccggtggcgt cctcgagccc gccgatccga acccggcgag gcttcccgca
     5221 tgcattggat attccccgga cccccgtgtg agcgcgtgga acgcgcggcc ccgatctgca
     5281 ggcaagtgaa acgctcgaaa cggaacgcgg actatatccc aaagtccctc gcccgccccg
     5341 ggcgatggtg cgccagagct ccgcccgtca atccgggatc gctccctcgg aagtccggat
     5401 ccgggggttg agcataccgc aagcgatggt tcgcggcgcc gccgaacgcg ggggcggatt
     5461 ggccggaaga ttgcatacgg ttcgtgccgg ttcgcggttg ctcgagtgcc ctggtccgga
     5521 cggctctggc gtatgcagtg aagacgcata cccggctcgg ggttgccctg ccgcctcccg
     5581 ggagaggggc aaggacaccc cgtggccaac gaaggccggc aggacgctgc gccggcgggc
     5641 cccaaaaacg tgatgaagtt ccttgtcaga tcgtttcctg tagcatagaa tgaggccccc
     5701 cgggggcgac gtctatgggc aagctggatg cttgtggtgg aataggcgaa cggcgtcggt
     5761 tgccggtccg tggcgtggag ggatcggccg ggatcgttac cttccgtaca tgggcggcca
     5821 tgtcggaacg gcgggggcta cgggggtagt ggcatgacgg gatttgtcac atttgcgctg
     5881 gccccggcgg gataccgcga ctgcgcgatt gcgatcgcgg cgtgccgggc aggggccgtg
     5941 ggtatcgtca atggcgagtt gaaccccgac cgctcggcgc tggcccgcga actggacgag
     6001 gtctcccggc gcgccaaggg cccctacggc atcaagctcg acgcgatgga tgccgccctg
     6061 gagggcgcgt tgcgcgccca cgcagcagcc ggcctgcgat gggtcgtcct cgattgcgcc
     6121 cttgtcgcgg ccgtccccgg cctggtttcg gacttgcgcg ggcagggcgt gcgggtgctg
     6181 ggcgaagtca cgaccccgga ttggcccggc cagccgttag acggcgttct cgacggattg
     6241 tggatcaagg ggaacgaatc cggcgggttc gtcggcgaag aagccagctt catcctcatc
     6301 cagaagtggt tgaagcgcac gcacctgccg ctgtacgtcc gtggaggcgt tactccgcac
     6361 tcggccgccg cttgcgcggc catcggcatc gccggggggg tgctggattc gcaattgctg
     6421 ctgttcgacg atctcggcct cttcgaaacg ttgaacccct tgctgcggag cctgtcgggc
     6481 agcgaaaccg ttgcggtcgg cgatggcgaa cgcggcgagt acttcagggt gctggtgcgg
     6541 cccggcaacc cggccgcgcg cgagttcgtc gccgccggcg aatccgaatg cgcagaaggc
     6601 ctgcggcccc tgctggccgg ccggaccgat tggacccggc cgacccgttc gctgctcccg
     6661 atcggccagg atgtggcctt cgcggccgac tggcgcgtgc ggtatggaag cctgcatgcc
     6721 ctcgtcgcgg cgatcgatga cgcggtggcg gaccacctgc gggctgccgt cgcatgccat
     6781 ccaattcgcg aaaatggtcc gctggcgcag gcattgggca tccgtgttcc ggtcgtgcag
     6841 ggtccgatga cgcgcgtctc ggacactgcg gagttcgcgt tgtccgtggc ggacggcggg
     6901 gcattgccca tggtcgcgct ggcattgatg aaagaggccg cgctcgactc gctattggcc
     6961 cgcacggcgg agctgctgga agatcgcccg tggggcatcg gcctgctggg atttgcgccg
     7021 caatcgctgc tcgatgagca ggtcgctgtc gcgctcaagt acaagcccga ttacgcgatc
     7081 atcgccggcg gacgccccga ccaggcggta cgcctggacc aggcgggcat tcccagcttc
     7141 ctgcacgtac ccagcgcccg gctgatcccg atgttcatgc aggagggtgc gcgccgtttc
     7201 atcttcgagg gccgcgagtg cggcgggcac atcggtccgc tgagcagctt cgtgctgtgg
     7261 agcgcgatgg tcgatgcgct gctggcggag ctgtccggcg gcaagtacga gccgtccgaa
     7321 ctccagttgt tgttcgcggg cggcatccac gatgcggtgt cgtcggca
//
LOCUS       DASSNH010000095        20276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_767220, whole genome shotgun sequence.
ACCESSION   DASSNH010000095 DASSNH010000000
VERSION     DASSNH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767220"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1069
                     /gene="rpoA"
                     /locus_tag="VFK00_03340"
     CDS             71..1069
                     /gene="rpoA"
                     /locus_tag="VFK00_03340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7592020.1"
                     /translation="MAVSSTQVLRPRGLKVEPVGPNHARVVVEPLERGYGHTLGNALR
                     RVLLSSISGAAIVEAEIDGVLHEYTTLEGLQEDVIEVLLNLKGVALRLNGVDESTLTL
                     SKKGKGVVTAGDITTDHTVEIINPEHVICHLTKDVSLNMRLKARRGIGYQPAVTRVNP
                     DEEARPIGRLQIDASFCPVRRVAYSVDAARVEQRTNLDKLVLDVETNGTVAVDEAVRQ
                     AAQTLHEQLSVFGDFKSRDMTESEKDKTGVSPLLLRPIDDLELTVRSANCLKAESIYY
                     IGDLVQKTEVELLKTPNLGKKSLTEIKDVLGAKGLALGTKLENWPPAGISSGMQLG"
     gene            1496..1879
                     /gene="rplQ"
                     /locus_tag="VFK00_03345"
     CDS             1496..1879
                     /gene="rplQ"
                     /locus_tag="VFK00_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7592021.1"
                     /translation="MRHLKSGRKFNRTSSHREAMYKNMTASLIKHELIRTTLPKAKDL
                     RRVAEPLITLAKVDGVANRRLAFARLRDKQAVGKLFVELGPRYRERAGGYLRIIKCGN
                     RVGDNAPMAYVELVDRPQAPAATEE"
     gene            2302..2853
                     /locus_tag="VFK00_03350"
     CDS             2302..2853
                     /locus_tag="VFK00_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HET7592022.1"
                     /translation="MNPFRWSFRAAFLLGFLVCVALLGYALYAERQLNLVPCNLCILQ
                     RVAFVWMGLWFLVGGLHAPRAGGRWAYVTLVLVGAAFGVAVAARQLWLQSLPADQIPS
                     CGAPYSMLLQQVHAGSLPFGRFVTLMFSGSGDCAQVTWRFLGLSMAGWTLIWYVLLAI
                     WALVPRPRRNDLRNAPPLRAMGR"
     gene            2856..4256
                     /locus_tag="VFK00_03355"
     CDS             2856..4256
                     /locus_tag="VFK00_03355"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HET7592023.1"
                     /translation="MAAPEGPSHATLRPVREAQPWQVDSWQARPAAQMPVYDDPAELQ
                     RALERLGQLPPLVTSWEILALKNLVAEAQAGKRFLLQGGDCAETFADCNNEVIANRLK
                     VLLQMSLVLVHGLRMPVLRVGRFAGQYAKPRSADTETIDGVTLPAYRGDLVNAPEFTA
                     AARRPDPMRMVEGHARSAMTMNFVRALVDGGFADLHHPEYWNLAWVERSPLAAEYRRM
                     VDGIGSAVRFMETLAGPIVGFMKVDFFTSHEALLLPYEQAMTRQVPRQWGWFDLSTHF
                     PWIGMRTAALDGAHVEFCRGIRNPIAIKVGPAMHADQLRQLIDILNPDDEAGRLTLVH
                     RMGAGGISQHLPPLIDAVKADGRRVLWLCDPMHGNTEKLGSGVKTRRFANIRSELEQA
                     FEIHAAAGTRLGGVHLELTGEDVTECMGGARDLSESDLARAYKSTVDPRLNYEQALEL
                     AMLVVRKSDGKNPRGS"
     gene            complement(4307..4837)
                     /locus_tag="VFK00_03360"
     CDS             complement(4307..4837)
                     /locus_tag="VFK00_03360"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HET7592024.1"
                     /translation="MPARPRQILPDVLEPDLRVVFCGTAPGTRSAMEGAYYAHPGNYF
                     WRTLFEVGLTPVRLAPPEYRRVLEFGIGLTDVAKHHFGSDADLPMSAFDAASLRRKLA
                     RYKPRIVAFTSKNAARAGLAGGSRHLACGEQDERIGQSRVFVLSSPSGQARGFWNIEP
                     WRELARAVAERQDASG"
     gene            4978..6003
                     /locus_tag="VFK00_03365"
     CDS             4978..6003
                     /locus_tag="VFK00_03365"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET7592025.1"
                     /translation="MDPSAAGGIHILPIHYQAPGLYAGFWRRVAAWLIDMLVIGTFHW
                     LLVFSFGTWLLVPWAMLGGAHGAAAARLLDFGLQPLGIVIFWLYFAAFESSSVQATPG
                     KLALGLIATDEYGRRIGFARATGRYFGKFVSALILGIGFLLAGWTARKQALHDLMAGC
                     CVVRKDGLAAWRSGLPSDAGAATASAAPMHAAQPAHTGMPGWAIALIVIAACFFLVPV
                     AAIVTAIAIPAYQSYTVRAEVSEGLRVTERARALVAEYIGQRGALPDSNIDLGLPRPE
                     AIRARYVSSVRVTGGRVVVTYGNQADSLIRGGHVVISPQGNAAMLQWRCSSPDIDARY
                     LPRQCKD"
     gene            complement(6056..7903)
                     /gene="typA"
                     /locus_tag="VFK00_03370"
     CDS             complement(6056..7903)
                     /gene="typA"
                     /locus_tag="VFK00_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7592026.1"
                     /translation="MSIQKLRNIAIVAHVDHGKTTLVDQLLKQSGTLSERAAVPDRVM
                     DSNDIERERGITILSKNTAIRWSDPAGEQWRINIVDTPGHADFGGEVERVLSMVDSVL
                     ILVDAMDGPMPQTRFVTQKAFQMGFKPIVVVNKVDRPGARPDWVVNQTFDLFDRLGAS
                     EEQLDFTVVYASALQGYAGLDPEVRSGDMTPLFDAIVRHVQAPQVDPDGPFQMRVSSL
                     DYSSYVGVIGVGRVQRGKVRTNMPVVIVDREGKRRNGKVLQVLGFMGLERREVPEAQA
                     GDIIAVSGIEDLGISDTICDPAGVEALPVLTVDEPTISMTFQVNNSPFAGNKDLSGGK
                     FLTSRQIRERLARETLHNVALRVEDTADPDKFKVSGRGELHLSILIETMRREGYELAV
                     SRPEVIVREVDGVLQEPWEQLVVDLEEQYQGGVMEKLGARRAQIRNMEPDGKGRVRLE
                     YLIPARGLIGFQTEFRTLTAGTGLMFHVFDHYGPKSEGAIGKRPYGVMISNGTGPAPA
                     YALYGLQDRGRMLIDAGVQIYEGQLVGIHAKDNDLTVNALRAKQLTNVRAAMKDDALT
                     LTPYVKLSLEQALEFIDDDELVEVTPKEIRLRKKHLTENDRKRASRQAA"
     gene            8064..8576
                     /locus_tag="VFK00_03375"
     CDS             8064..8576
                     /locus_tag="VFK00_03375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7592027.1"
                     /translation="MTDLNATFQTSKGPIHVRLFADEAPVTVASFVNLAQRGYYDGLD
                     FHRVIPDFMIQGGCPLGTGTGGPGYQFEDECRADLKHDAPGKLSMANAGPRTNGSQFF
                     ITHVPTPWLDGKHTIFGEVLSAGDQNVVNQIRQGDTIEKVTIEGDAAPLLAAQADRIA
                     QWNAALDARS"
     gene            complement(8628..8912)
                     /locus_tag="VFK00_03380"
     CDS             complement(8628..8912)
                     /locus_tag="VFK00_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7592028.1"
                     /translation="MLHWLWIFLIGLVVGIIAKWLMPGKDPGGFIITAIIGIVGSLIG
                     GWIASAVFGVGAGATAEGAGIGGLWGLVFGVIGAIVLLIIYRLIFRPKRA"
     gene            9078..10319
                     /locus_tag="VFK00_03385"
     CDS             9078..10319
                     /locus_tag="VFK00_03385"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7592029.1"
                     /translation="MPRLAARTRRAKPSAIMIIADTARQLKAEGKDVVNFSIGVPNFL
                     PGEHVYAAVREWLPADSGGYGTNRGSPALLDAIVAHYRDSGLDGYARNNVTVGIGAKQ
                     VLYNLCEALLDEGDEIVFPAPYWTSYVDIADILGAKATVLPCPPEQHYKLTPAQLDKA
                     LASRPKVFLFNNPSNPTGMVYTREEIAALADVLVKHPDTWIITDDIYNRLVFDGIGYH
                     NFVQFKPELRERTFIMDSLSKTYGMPGWRVGFVAGPEAGVKALVTLNSNHITCLPEVV
                     TAAAVAALAGPQDVPMAKREEFSRRRDEVVGALTAIPGVKCPRPQGAFYAFPDISVAF
                     GKYHKGRKIASDVDFCKVLLEEKFVATVPGSAFGEPRSLRISYTCPMTQVRKGLDRIR
                     EFFGELSEESAVAGPASAVGA"
     gene            10402..11382
                     /locus_tag="VFK00_03390"
     CDS             10402..11382
                     /locus_tag="VFK00_03390"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7592030.1"
                     /translation="MKPPVRVAVTGAAGQIGYALLFRIAAGDMLGPDQPVILHLLEIT
                     PALPALNGVVMELDDCAFPLLAGVVATDDANVAFKDIDYALLVGARPRGAGMERKDLL
                     SANGAIFGPQGKALNAHAKRDVKVLVVGNPANTNALIAQANAPDLDPKCFTAMVRLDH
                     NRAKAQLAAKAGAHNTDVRKMIIWGNHSSTQYPDIHHATVKGKPALSLVDQAWYEKEF
                     IPTVQQRGAAIIKARGASSAASAASAAIDHMHTWAHGTAEGDWVSMGIPSDGSYGIEP
                     GVIYGYPVTVKDGKYTIVQDLEIDGFSRARMDATDKELREERAAIGDLLG"
     gene            11523..13781
                     /locus_tag="VFK00_03395"
     CDS             11523..13781
                     /locus_tag="VFK00_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592031.1"
                     /translation="MGSRGFWQELKRRHVYRVAAAYVVVGWVLIQVVTQVFPIFHLPD
                     WIDQAIVLLILIGFPIALVLAWAFDAVPRGNVTAYGPADSDGPSLPLRRRSRRAGLIV
                     GAIGVLVAVIAGGAYWHFGRDGSRAVPATVAATGRAARGPDGAQRNQGSGNADSTAYS
                     VASGLHDASAANAAPIAPQPIPSKSIAVLPFENLSTDKGNAYFADGIQDLILTKLADI
                     GGLKVVSRTATENDSSHPDDLSTIGHRLGVATFLEGTVQKAGNQVLINVQLIDAKTDN
                     HIWARSYQRDVTNIFGVEGEVAGKVADALNAKLTAAESAAVAHVPTTNPQAYDDYLRG
                     LHYDHEAGKGDWTTFLPQAIAAYGKAVAGDPNFALAWAALSNARSDAYFWAGDHSKAN
                     LLAADTSARRALQLDPRLADAHIAMAGVERLLQHDLVAAHSQMQRAVELRPNDADALA
                     NLAISDANLGIAGNGKLLQRAIALDPTDPFNYYQAGVGLAAAGDYAGARQAERRALAI
                     DPQFVLAYLALSRIEISDSQNVEAATKILEHMAPGTPVNVSVVIWRIKLLLFRRQYDA
                     ARALAGKFAGKFASGPGAVDMTFARANIEWLAGKVDPARGLYRTAIGLVTRPGSEISG
                     ADHARLGVAYARLGNADAAIKEGNEALTMAVKSHQHDAPQRVKYDLASARLALGHESA
                     AVEALTEVLSMDPSDIRAPWLLFLARMQLAPTWDPIRNDPRFQALLKEYAATAASTSN
                     APTAAGARGTNG"
     gene            13865..14140
                     /locus_tag="VFK00_03400"
     CDS             13865..14140
                     /locus_tag="VFK00_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592032.1"
                     /translation="MAATHVVVGWLVIQVVTVIFPLLQFPPWPARDIVLLVLVLVGFP
                     IVLVLAWGLQTPRDERGASASVRRRSLRTGFTRGGVGVLIAMLAGAV"
     gene            complement(14896..15840)
                     /locus_tag="VFK00_03405"
     CDS             complement(14896..15840)
                     /locus_tag="VFK00_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7592033.1"
                     /translation="MLSATPPQGAHVMEDGTQSHSPLSSAKSFVQEFLGDKPFQLAAA
                     LSYFTLLSMVPLFLLLTGVAGLVFGREAAREQLLAKVGSMVGQQQADVVRALLSQSGN
                     HQASVISTIIGTAILVFGATTVFGHLQIALNQIWDVKAVPSRSAIWRLLGSRLLGLAI
                     VLGMAFILLVSMAASAALAGAHGYLSGLFPGAGTVLRVVYALVSLAIISGVIAMLFKY
                     VPDAVIDWRDVIVGALITGVLFTIGKYALGVYLAHLRYSSAGAAGSLILLVVWVYYSA
                     LILFLGAEITQFYTCRYGKPITPRAHARRAQQKLKTTS"
     gene            15977..16867
                     /gene="prpB"
                     /locus_tag="VFK00_03410"
     CDS             15977..16867
                     /gene="prpB"
                     /locus_tag="VFK00_03410"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HET7592034.1"
                     /translation="MGASSSAGARFRAALAAETPLQVAGAITAYAGLMARRVGYRALY
                     LSGGGVAANSLGMPDLGISTMEDVLTDARRIVDATRLPLLVDIDTGWGGAFNIARTIR
                     SFERIGVAAVHMEDQVGQKRCGHRPGKEVVPTGEMVDRVKAAVDAKIDRDFVLMARTD
                     AAAVEGIDAAIERAVAYVEAGADMIFPEAMKTLDDYRKFRAAVKVPILANLTEFGATP
                     FFTVDELRSANVDIALYCCGAYRAMNKAALGFYETVRREGTQKSVVPAMQTREELYDF
                     LGYHAYEKKLDELFAGGSKD"
     gene            16874..17452
                     /locus_tag="VFK00_03415"
     CDS             16874..17452
                     /locus_tag="VFK00_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592035.1"
                     /translation="MSDSTPPQDDPQHESARRGVRWDALAAIIASLVGLLALIVAGYT
                     AYIERQQVRAQVWPYLQAGYSGTFHRYIWINRGVGPAVVKSVQVLVDGKPQQNWKTVQ
                     QALRIPSFQYHQSGLSGTVVSPGQTLQWLQFEEKDEYEDFRRAAERASLQTKVCYCST
                     LGDCWMNDSAKKDPRVSVEQCPALPESQQFDD"
     gene            17459..18082
                     /locus_tag="VFK00_03420"
     CDS             17459..18082
                     /locus_tag="VFK00_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592036.1"
                     /translation="MSTPPSNSDHPVPRQRRRREVEWDAVAAIIASLVGFLALLVAGY
                     TAYVERYTAEIQNKQVQAQVWPWLEAGNNDDTGSIEVVNKGVGPAIVRSAQIFVDGKP
                     QRDWQHVLAALGAAQHGFSQMTLNPNVLAPGEKVAAIRFKNKDDYARFRAAAVAHLTL
                     INICYCSSLGDCWRYSDQHLVGYKSLAKLVTPVAQCPKLPRDAVFIN"
     gene            18123..19274
                     /gene="prpC"
                     /locus_tag="VFK00_03425"
     CDS             18123..19274
                     /gene="prpC"
                     /locus_tag="VFK00_03425"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HET7592037.1"
                     /translation="MSDNAQVLPKAKKSVALSGVAAGNTALCTVGRSGNDLHYRGYDI
                     KDLATRATFEEVAYLLVHGHLPTWAELNAYRAKLKRLRGLPQPVRAALELLPASTHPM
                     DVMRTGCSVLGTVLPEAPDHNVIGARDIADHLMASFGSMLLYWYHYSHNGRRIEVETD
                     DESLAGHFLHLLHGRAPGELHANALDKSLVLYAEHEFNASTFTARVIAGTGSDRYSAI
                     TGAVGALRGPKHGGANEVAMEIISRYRTGDEADADIRARVERKEIIIGFGHPVYTVSD
                     PRNEIIKEISRKLCTEGGNPRLFEVSERIERVMGEVKKMFPNLDWYSASVYHMLGVPT
                     AMFTPLFVIARTSGWSAHVIEQRQDGKIIRPSANYTGPEDQAYVPMEKR"
     gene            complement(19549..20172)
                     /locus_tag="VFK00_03430"
     CDS             complement(19549..20172)
                     /locus_tag="VFK00_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7592038.1"
                     /translation="MKLYSFSGSCALASHIVLEWIGKPYTVQLMQKEELSRPAYRRLN
                     PNGQVPTLEEDDGWILFENAAILNYLADKYPDAKLGGDDTPRGRAEVNRWLALINSDM
                     HPAFKPLFGTTAFLGDAAAIEKTKENARKRLRGYFELVDAQLGKHDWLAGTRSIADPY
                     LFVLVRWSRAVKVDLSGLENVKKFEQRMRADPAVQNALKGERLDQAA"
BASE COUNT         3526 a         6664 c         6627 g         3459 t
ORIGIN      
        1 accgtgccgg tgttgaacca tcggagcccg aatcctgcgc ggctccacca gtgatcttcc
       61 ggagtagccc atggcagtct cttccaccca ggttttgcgc ccgcgcggtc tcaaggtcga
      121 gccggttgga cccaatcatg cacgcgtcgt cgtcgagccg ctcgagcgcg gctacggcca
      181 cacgctcggc aatgcgctcc ggcgcgtgct gctgtcctcg atttccggcg cggcgatcgt
      241 cgaagccgaa atcgatggcg tgctgcatga atacaccacg ctcgaagggc tgcaggaaga
      301 cgtgatcgag gtgctgctga acctgaaggg cgtcgcgttg cgcctgaatg gcgtcgacga
      361 atcgaccctc accctgtcga agaagggcaa gggcgtggtc accgcgggcg acatcacgac
      421 cgaccacacg gtcgagatca tcaatcccga gcacgtgatc tgccacctga ccaaggacgt
      481 gtcgctgaac atgcgcctga aggcccgccg cggcatcggc taccagccgg ccgtgacccg
      541 ggtgaacccg gacgaggaag cccgacccat cggccgcctg cagatcgatg cttcgttctg
      601 cccggtccgc cgcgtcgcgt attctgtcga cgccgcgcgc gtcgagcagc gcaccaacct
      661 cgacaagctg gtgctggacg tcgagaccaa tggcaccgtc gcggtcgacg aggctgtgcg
      721 ccaggccgcg cagaccctgc acgaacaact gtcggtgttc ggtgacttca agagccgcga
      781 catgaccgag tccgagaagg acaagaccgg cgtgtccccg ctgctgctgc gcccgatcga
      841 cgatctcgag ctcaccgtgc gttcggcgaa ctgcctcaag gccgagagca tctactacat
      901 cggcgacctg gtgcagaaga ccgaggtcga gttgctgaag accccgaacc tcggcaagaa
      961 gtcgctcacc gagatcaagg acgtgctggg tgcgaagggc ctcgcgctcg gcaccaagct
     1021 cgagaactgg ccgccggcgg gaatttcgtc ggggatgcag ctgggttgag gtaggagatg
     1081 ccttcgtgcc catatctccc gtgtgtttat ttgtgcgatc ttccctgatc gctgcgtcac
     1141 gaacggctgg gtaatcttca acggtttctc ggtcggcaac accagaacgg gcatgttttt
     1201 gcgatcgtcc ctgatcgcgg catcacctcc gattgagtaa ttttcaagga tttcccagtc
     1261 ggaaacatca gaacgggctt cctgccctga cttctcacat gtttgtgcga tcgtccctga
     1321 tcgccgcgtc aagaaccatc gagtcccttt agacgattcg atggttggca gcaccagaac
     1381 ggccatccat ggcctgactt ttcacaacag ctcgcttcga cgagcctcaa tagcggaatt
     1441 ccgcgggact ccggacatag gcgccggctt cccataacga taaagagaga aaaccatgcg
     1501 ccacctcaaa tccggacgca agttcaaccg gaccagctcc caccgcgagg ccatgtacaa
     1561 gaacatgacg gcctcgctga tcaagcacga gctgatccgc accacgctgc ccaaggccaa
     1621 ggacctgcgc cgcgttgccg agccgctgat cacgctggcc aaggtcgacg gcgttgccaa
     1681 tcgccgcctc gcattcgcgc gactgcgcga caagcaggcg gtcggcaagc tgttcgtgga
     1741 actggggccc cgctaccgcg aacgggcggg cggctacctc cgcatcatca agtgcggcaa
     1801 ccgcgtcggc gacaacgcac cgatggcgta tgtcgaactg gtcgatcgtc cgcaggcgcc
     1861 ggccgccacc gaagagtgac cgcccaaatc gcttcacgca aaaaaaaccc cggcttgccg
     1921 gggtttttgt tttcgcgagc tcgcaaaacg gcccaagccc gcgggtttga ggcggcatgc
     1981 tccggtgcgc ttgttcctgc tgtgcctttt tcggtccgta acggtctgcc gggtccgtcg
     2041 cttcgctcct tgacctgtct ccctgggtcg gcctgcatgg actggtcgca ggtcggggct
     2101 tggcatgtgt ccggcaaccg gtgcatcatc ggcgggtgga ctcgaccatc gcaacggatg
     2161 cggaggggcg gacgagccgc ggatcggctg cccggaacga tccacgggct cgtcgaccgg
     2221 ctgttggccc gagctgattc ctgctgacaa tcgccccgac ccgcgctaac gttgtggttc
     2281 tggtccgcag cagccacccc aatgaaccct tttcgctggt ctttccgcgc cgccttcctg
     2341 ctcggcttcc tggtttgcgt ggcgttgctg ggctacgccc tgtatgccga gcggcaactc
     2401 aacctggtgc catgcaacct ctgcatcctg cagcgggtgg cgttcgtctg gatggggttg
     2461 tggttcctcg tcggtgggtt gcacgcgccc cgtgccggcg gccgttgggc ttacgtcacg
     2521 ctggtgctgg tcggcgcggc gttcggcgtc gcggtcgcgg cgcgccagtt gtggttgcaa
     2581 tcgttgccgg ccgaccagat tcccagttgc ggcgcgccgt acagcatgct gttgcagcaa
     2641 gtccacgccg gctcgctgcc gttcggccgg ttcgtgacgc tgatgttcag cggctcgggt
     2701 gactgcgcgc aggtcacctg gcgcttcctc ggcctgtcga tggccggctg gaccctgatc
     2761 tggtacgtgc ttctcgcgat ctgggccctg gttccgcgtc cgcgccgcaa cgacttgcgg
     2821 aatgcaccgc cgttgcgggc gatgggaagg tgagcatggc cgctcccgaa gggccttcgc
     2881 acgccacgct gcgtccggtg cgcgaggcgc agccgtggca agtcgacagt tggcaggccc
     2941 ggcccgccgc gcagatgccg gtctacgacg atccggcgga attgcagcgg gcgctcgagc
     3001 ggctcggcca gttgccgccg ctggtgactt cctgggaaat ccttgcgctc aagaatctcg
     3061 tggccgaggc ccaggccggc aagcgcttcc tgttgcaggg tggcgattgc gccgagacct
     3121 tcgccgactg caacaacgag gtgatcgcca atcgcctgaa ggtgctgctg caaatgagcc
     3181 tggtgctggt gcacgggctg cggatgccgg tgctgcgggt gggccgtttc gccggccaat
     3241 acgccaagcc gcgctcggcc gacaccgaaa cgatcgatgg cgtgaccctg ccggcgtatc
     3301 gcggcgatct cgtcaatgca ccggaattca cggccgcggc gcggcgtccc gatcccatgc
     3361 ggatggtcga ggggcacgcc cgctcggcga tgaccatgaa cttcgtgcgc gcgctcgtcg
     3421 atggcggctt cgcggacctg caccatcccg agtactggaa cctcgcgtgg gtggagcgct
     3481 cgccgctcgc ggccgaatac cggcggatgg tggatggcat cggcagcgcc gtgcggttca
     3541 tggaaaccct cgcgggcccg atcgtcggtt tcatgaaggt ggatttcttc acctcgcacg
     3601 aagcgctgct gttgccctac gagcaggcga tgacgcggca ggtgccgcgc cagtggggct
     3661 ggttcgacct gtcgacgcat ttcccttgga tcggcatgcg caccgccgca ctcgatggcg
     3721 cgcacgtgga gttctgccgc ggcatccgca accccatcgc gatcaaggtc ggccccgcga
     3781 tgcacgccga ccagttgcgg cagttgatag acatcctcaa tcccgacgat gaagccggtc
     3841 gcctgaccct cgtccaccgc atgggcgcgg gtggcatcag ccagcacctg ccgccgctga
     3901 tcgacgccgt gaaagcggac ggacgacgcg tgctgtggct gtgcgacccg atgcacggta
     3961 acaccgaaaa gctcggcagt ggcgtcaaga cgcgccgttt cgccaacatt cgcagcgaac
     4021 tcgagcaggc gttcgagatc cacgccgcgg ctggcacccg gctcggcggc gtgcacctcg
     4081 aactgaccgg cgaggacgtg accgaatgca tgggcggcgc tcgcgatctg tccgaatccg
     4141 atctcgcgcg cgcctacaag tccacggtcg atccgcggct caattacgaa caggcgctgg
     4201 agctggcgat gctggtcgta cgcaagtccg acgggaagaa tccgcgcggc tcctgaagcg
     4261 gcgccagcgg gacgcgggat gcggccgttg gcttacggcc cttggctcag ccggaagcat
     4321 cctgacgttc ggcgacggcc cgggccaatt cccgccacgg ctcgatgttc cagaagccgc
     4381 gtgcctgccc cgatggcgag gacagcacga atacccggct ttgcccgatc cgttcgtcct
     4441 gttcgccgca ggcgagatgg cgcgagccgc ctgccagtcc cgcccgcgcc gcattcttgc
     4501 tggtgaaggc gacgatgcgc ggcttgtagc gcgcgagctt gcggcgcaag ctcgcagcgt
     4561 cgaaagcgct catcggcagg tccgcatccg acccgaagtg gtgcttggcg acgtcggtca
     4621 ggccgatccc gaactcgagc acccggcgat attccggcgg cgcgaggcgg accggcgtca
     4681 ggccgacctc gaacaaggtt cgccagaaat agttgccggg atgggcgtaa taggcgccct
     4741 ccatcgccga gcgcgtgccg ggcgcggtgc cgcagaacac cacgcgcaag tccggttcca
     4801 ggacgtcggg caggatctgg cgagggcgcg ctggcaccgc gggattgtga cacgcagggc
     4861 gcgaagcatg cggaatccgg atcgcgtgac gagcgtctcc ggacccaagc ttccggcaga
     4921 tgacggcatg ccgtttgccg tgcgaccatc accgccgtct tccccgatgc gcgacccatg
     4981 gacccctccg ctgccggcgg catccacata ctgcccatcc actaccaggc tccgggcctg
     5041 tacgccgggt tctggcgcag ggtcgcggcg tggctgatcg acatgctggt catcggcacg
     5101 ttccactggc tgctggtgtt ctcgttcggc acctggctgc tggtgccctg ggccatgctg
     5161 ggcggcgccc acggggcggc ggcggcgcgg ctgctggact tcggactgca gcccctcggc
     5221 atcgtgatct tctggttgta tttcgcggca ttcgaatcgt cgagcgtgca ggcaacgccc
     5281 ggcaagctgg cgctggggct catcgccacc gacgaatacg ggcgacggat aggttttgcc
     5341 cgcgccaccg gccgctactt cggcaagttc gtatcggcgc tcatcctcgg catcggcttc
     5401 ctgctggccg gctggactgc gcgcaagcag gcgctgcacg atttgatggc cggctgctgc
     5461 gtggttcgaa aggacggcct cgccgcgtgg cgaagcggac ttccgtccga tgcgggcgcg
     5521 gccaccgcgt ccgccgcacc gatgcacgcg gcgcagccgg cacacaccgg gatgcccggg
     5581 tgggcgatcg cactgatcgt gatcgcggcg tgcttcttcc tggtccccgt cgccgcgatc
     5641 gtcaccgcga tcgcgattcc cgcgtaccag agctacaccg tgcgtgcgga agtatccgag
     5701 ggactgcggg tgaccgaacg cgcgcgggcg ctggtcgccg aatacatcgg ccagcggggc
     5761 gcgctgcccg acagcaatat cgacctcggc ttgccgcggc cggaagccat ccgcgcgcgc
     5821 tatgtcagca gcgtccgcgt gaccggtggc cgggtggtcg tgacctatgg caaccaggcc
     5881 gattccctga tccgcggcgg ccacgtcgtg atctcgccgc agggcaatgc agcgatgttg
     5941 caatggcgct gcagcagccc ggatatcgac gcgcggtacc tgccgcggca gtgcaaggac
     6001 tgagccgcgc gcacaaccgg cgtgcggcga ctacccgagt cccaagtccc ggcggttacg
     6061 ccgcctgccg tgacgcgcgc ttgcggtcgt tctcggtcag gtgcttcttg cgcagccgga
     6121 tctccttcgg agtcacttcc accagttcgt cgtcgtcgat gaactccagc gcctgctcga
     6181 gcgaaagctt cacatacggc gtgagcgtca gcgcgtcgtc cttcatcgcg gcgcggacat
     6241 tggtgagctg cttggccctg agggcgttga ccgtgaggtc gttgtccttg gcatggatcc
     6301 cgaccaactg gccttcgtag atctgcacgc ccgcatcgat cagcatgcgg ccgcgatcct
     6361 gcaggccata cagcgcgtac gccggcgccg gtccggtgcc attcgagatc atcaccccgt
     6421 aggggcgctt gccgatggcg ccttcggact tcgggccgta gtggtcgaac acgtggaaca
     6481 tcaggccggt gccggcggtg agggtgcgga actcggtctg gaaaccgatc agtccccgcg
     6541 ccgggatcag gtattccaga cgcacccggc ccttgccgtc cggctccatg ttccggatct
     6601 gcgcgcgacg ggcgcccagc ttttccatca cgccgccctg gtactgctcc tcgaggtcga
     6661 cgaccagttg ctcccagggt tcctgcagga cgccgtcgac ctcgcgcacg atcacttcgg
     6721 gacgggacac cgcgagttca tagccttcgc ggcgcatcgt ctcgatcagg atcgagaggt
     6781 gcagctcgcc gcgccccgac accttgaact tgtccgggtc ggccgtgtcc tcgacccgca
     6841 gcgcgacgtt gtgcagcgtc tcgcgcgcga ggcgttctcg gatctgccgc gaggtgagga
     6901 acttgccgcc cgagaggtcc ttgttgccgg cgaacggtga attgttgacc tggaaggtca
     6961 tcgagatggt gggttcgtcc accgtcagca ccggcagtgc ctcgacgccc gcgggatcgc
     7021 aaatggtgtc ggaaatgccg agatcctcga tgcccgagac cgcgatgatg tcgccggcct
     7081 gcgcctccgg cacttcgcgg cgttcgagtc ccatgaagcc gagcacctgc aggaccttgc
     7141 cgttgcgccg cttgccttcg cggtcgacga tcacgaccgg catgttggtg cggaccttgc
     7201 cgcgctgcac gcgcccgacg ccgatcacgc cgacatagct cgagtagtcg agcgaggaaa
     7261 cccgcatttg gaacggaccg tcggggtcga cctgcggcgc ctgcacgtgc cggacaatgg
     7321 catcgaacag cggcgtcatg tcgcccgagc gaacctcggg gtcgagcccc gcgtagccct
     7381 gcagcgccga cgcgtacacc accgtgaagt cgagctgctc ttcgctcgcg ccgagccggt
     7441 cgaacaagtc gaaggtctgg ttgaccaccc agtccgggcg agcgcccggg cggtcgacct
     7501 tgttgacgac cacgatcggc ttgaagccca tctggaacgc cttctgggtc acgaagcgcg
     7561 tctgcggcat cgggccgtcc atcgcatcga cgaggatcag caccgagtcg accatgctca
     7621 acacgcgctc gacttcgccg ccgaagtcgg catgtccggg cgtgtcgacg atgttgatgc
     7681 gccattgctc gccggccgga tcgctccagc ggatcgcggt gttcttcgaa aggatcgtga
     7741 tgccgcgttc gcgctcgatg tcgttcgagt ccatcacgcg gtcgggcacc gccgcgcgtt
     7801 ccgacaaggt gccggactgc ttcaggagct gatcgaccag cgtggtcttg ccgtgatcga
     7861 cgtgggcgac gatcgcgatg ttgcggagtt tctggatgga cataggtaca cgtgacggcc
     7921 gcgcagcggc ggtcggcggg aaatggggag gagaatgggg cacgccctgc cgtgcagacc
     7981 cgcaattata taccgggcgg ccccggcggt gcttccagcg gcggcgactg ttaagctagc
     8041 agattgcctg cagggagctg cccatgaccg acctcaacgc caccttccag acctccaaag
     8101 gcccgatcca cgttcggctg ttcgccgacg aggcgcccgt gaccgtcgcc agtttcgtca
     8161 acctcgccca gcgcggctac tacgacggcc tcgacttcca tcgcgtgatc cccgatttca
     8221 tgatccaggg cggctgcccg ctgggcaccg gcaccggcgg tcccggttac cagttcgagg
     8281 acgaatgccg cgccgacctc aagcacgatg cgcccggcaa gctgtcgatg gccaacgcgg
     8341 gcccgcgcac caacggcagc cagttcttca tcacccacgt gccgacgcca tggctggatg
     8401 gcaaacacac catcttcggc gaagtgctgt ccgccggcga ccagaacgtg gtcaaccaga
     8461 tccgccaggg cgacacgatc gagaaggtca cgatcgaggg cgacgcggca ccgttgctcg
     8521 ccgcgcaggc cgatcgcatc gcgcagtgga acgctgcgct cgacgcgcgt tcgtgacgcc
     8581 atcgaccatg cgagcgaaga gggcggccac gagggccgcc cttccgatca cgcgcgcttc
     8641 ggcctgaata tcaggcggta gatgatcagc aggacaatcg cgccgatcac cccgaagacc
     8701 aggccccata agccgccaat gcccgcacct tcggcggtcg cgccggcacc gacgccgaac
     8761 accgcgctgg cgatccagcc gccgatgagc gagccgacga tgccgatgat cgcggtgatg
     8821 atgaagccgc cgggatcctt gccgggcatg agccacttgg cgatgatgcc gacgacaagc
     8881 ccgatgagga aaatccagag ccaatgcagc atgtggtttc tcctttgtag gtgaggggac
     8941 acccaaacgc cattcgtgca tggcgtgggc attgtgcacc gtcgcgaagt gttctcgacg
     9001 tgaacgtccg cgggtgccgt gatgcgtcgc agcatcgctg tggtaaaatg tggcgttgca
     9061 ctcgcgagga attttccatg cccagactcg ccgcgcgcac ccgccgcgcc aaacccagcg
     9121 cgatcatgat cattgccgac accgcccgcc agttgaaggc ggagggcaag gacgtcgtca
     9181 atttctcgat cggcgtgccg aacttcctgc cgggcgagca cgtctacgcg gcggtccgcg
     9241 aatggttgcc cgccgacagc ggcggctacg gcaccaaccg cggcagcccg gcgttgctcg
     9301 atgcgatcgt cgctcattac cgtgacagcg gtctggacgg ctacgcgcgc aacaacgtca
     9361 cggtaggcat cggggccaag caggtgctct acaacctgtg cgaggcgctg ctggacgaag
     9421 gcgacgagat cgtgttcccg gcgccgtact ggacgagcta cgtcgacatc gccgacatcc
     9481 tgggcgcgaa ggccacggtc ctgccatgcc cgccggagca gcactacaag cttaccccgg
     9541 cgcagctcga caaggcgttg gcgtccaggc ccaaggtctt cctgttcaac aacccgtcga
     9601 atccgaccgg catggtctac acgcgcgagg agatcgccgc gctggccgac gtgttggtga
     9661 agcatcccga cacctggatc atcaccgacg acatctacaa ccgcctggtg ttcgacggca
     9721 tcggctacca caacttcgtg cagttcaagc ccgaactgcg cgaacgcacc ttcatcatgg
     9781 actcgctgtc caagacctac ggcatgcccg gttggcgcgt aggtttcgtc gccggccccg
     9841 aagccggcgt gaaggcgctg gtgacgctca actccaacca catcacctgc ctgccggaag
     9901 tcgtcaccgc tgccgcggtc gcggcgctcg cggggccaca ggacgtgccg atggccaagc
     9961 gcgaggaatt ctcccggcgc cgcgatgaag tggtcggtgc actgacggcg atccccggcg
    10021 tgaaatgccc gcgtccgcag ggtgcgttct acgcattccc cgacatctcg gtcgcgttcg
    10081 gcaaatacca caagggccgc aagatcgcca gcgacgtcga tttctgcaag gtgctgctcg
    10141 aagagaaatt cgtcgcgacg gtgccgggtt cggcattcgg cgagccgcgt tccttgcgga
    10201 tttcctacac ctgcccgatg acgcaggtcc gcaagggcct ggaccgcatc cgcgaattct
    10261 tcggcgaact gagtgaagaa tccgccgttg cagggccggc cagcgccgta ggcgcgtaac
    10321 cggccggtca tccatacgcc atcggcgggg cacgccgttt tacggccaac ccgcctacga
    10381 catgtcttac ggagttcgag aatgaagcca cctgttcgcg ttgcagtcac cggcgccgcc
    10441 ggccagatcg gctatgcgct gttgttccgg atcgccgcgg gcgacatgtt gggccccgac
    10501 cagccggtga tactgcacct gctcgaaatc acgcctgcgc tgccggcgct gaatggagtg
    10561 gtgatggaac tcgacgactg cgcgtttccg ctgctggcgg gcgtggtcgc gaccgatgac
    10621 gcgaatgtcg ccttcaagga catcgactac gcgctgctgg tcggtgcccg cccgcgcggc
    10681 gccggcatgg agcgcaagga cctgctctcg gcgaacggcg caatattcgg gccgcagggc
    10741 aaggcgttga acgcgcacgc caagcgcgac gtcaaggtgc tggtggtcgg caacccggcc
    10801 aacaccaacg cgctgatcgc gcaggccaac gcgccggacc tcgatccgaa gtgcttcacc
    10861 gcgatggtca ggctcgacca caaccgcgcc aaggcccagc ttgccgccaa ggccggcgcg
    10921 cacaacaccg acgtccgtaa aatgatcatc tggggcaatc actcctcgac ccagtacccc
    10981 gacatccacc acgcgacggt gaagggcaag ccagcgctgt cgctggtcga ccaggcgtgg
    11041 tacgagaagg aattcatccc gaccgtgcaa cagcgaggcg ccgcgatcat caaggcgcgc
    11101 ggtgcgtcgt cggcggcatc cgccgcgtcg gcggcgatcg accacatgca tacctgggcg
    11161 cacggcacgg ctgaaggcga ctgggtatcc atgggcattc cgtccgacgg ttcctacggc
    11221 atcgagcccg gcgtgatcta cggctacccg gtcacggtca aggatggcaa gtacaccatc
    11281 gtgcaggatc tcgagatcga tggattctcg cgcgcacgca tggacgccac cgacaaggaa
    11341 ctgcgcgaag agcgcgcagc gatcggcgat ctgctcggct gaagcgcgac acctcctgtt
    11401 gacccgcaag ggcggtgctg tgccgccctt cgcgcttcgc ctgaaagtgc tgccgtaccg
    11461 tgccagaatg acggcgcatc gcaggcaggg accgccgcgc accccgacat cggagccggc
    11521 gcatgggcag cagggggttc tggcaggaac tgaagcgccg ccacgtctat cgcgtggccg
    11581 cggcctatgt ggtcgtcggc tgggtgctga tccaggtggt cacgcaggtg tttccgatct
    11641 tccatctgcc cgactggatc gatcaggcca tcgtgctgct gatcctgatc ggctttccga
    11701 tcgcattggt cctggcgtgg gcattcgatg cggtgccacg agggaacgtg acggcgtacg
    11761 gaccggcgga cagcgacggc ccgagcctac cgttgcgccg tcgctcgcgc cgcgccggcc
    11821 tcattgtcgg tgcgatcggc gtattggtcg ccgtgatcgc gggtggtgcg tattggcatt
    11881 tcgggcgaga tggatcgcgt gccgtgccgg caaccgtggc agcgactggg cgcgcagcgc
    11941 gtggcccgga tggagcgcag cgcaatcagg gatcggggaa cgcggattcc accgcttact
    12001 cggttgcatc cgggctgcat gacgcgtctg ccgcgaatgc agcgccgatc gccccccaac
    12061 ccattccctc caaatccatc gcggtgctgc cgttcgagaa tctttccacc gacaagggca
    12121 acgcctactt cgccgatggc attcaggacc tgatcctgac caagctggcc gatattggtg
    12181 gcctcaaggt ggtctcgcgc acggccaccg agaatgactc cagccacccc gatgacctga
    12241 gcaccatcgg gcaccggctc ggcgtcgcga cgttcctcga aggcaccgtg cagaaagcgg
    12301 gcaatcaggt gttgatcaat gtgcagttga tcgatgcgaa aaccgacaac cacatctggg
    12361 cgaggagtta ccagcgcgac gtcaccaaca tcttcggggt cgagggcgag gtggccggga
    12421 aggtcgccga cgcgctgaac gccaagctta ctgctgccga gagtgccgcc gtggcgcacg
    12481 tgcccaccac caacccgcag gcctacgatg actacctgcg cggcctgcat tacgaccacg
    12541 aggcgggaaa gggcgactgg acgaccttcc tgccgcaagc cattgctgcc tacgggaagg
    12601 ccgtcgccgg ggatcccaat tttgcgctgg catgggcggc gctatcgaac gcgcgcagcg
    12661 atgcgtattt ctgggccggg gatcactcca aagcgaacct gcttgcggca gatacatccg
    12721 cccgacgggc cttgcaactc gatccaaggt tggccgatgc ccacattgcc atggccggcg
    12781 tcgaaaggct cctccagcac gatctggtcg ccgcacatag ccagatgcag cgggcggtcg
    12841 agcttcgtcc gaacgacgcg gacgccctcg cgaacctggc catctccgac gccaacctcg
    12901 gcattgccgg gaacggcaag ctgctgcagc gtgcaatcgc gctcgatccc accgaccctt
    12961 tcaattacta ccaggcggga gtggggcttg cggcggcggg cgactatgcc ggagcgcggc
    13021 aggcagagcg gagggcactg gcgatcgacc cgcaattcgt tctcgcctat cttgccttga
    13081 gccggatcga aatctccgac agccagaacg tcgaggccgc cacgaaaatt ctcgaacaca
    13141 tggcgcccgg cacgccagtg aacgtctcgg tcgtgatctg gcgcatcaag ttgctgctgt
    13201 tccggcgcca atacgatgcc gcgcgcgcac tggccggaaa atttgccgga aaattcgcaa
    13261 gcggtccggg ggcagtggac atgacttttg cccgggccaa tatcgaatgg ctggcaggca
    13321 aggtggaccc tgcccgtgga ctctaccgca cagcgatcgg cctggtgacc cggccgggtt
    13381 cggaaatcag tggggcggac cacgctcgac tcggtgttgc gtacgcgcgg ctcgggaacg
    13441 cggacgccgc catcaaggaa ggcaatgaag cgctcacgat ggccgtgaag tcgcaccagc
    13501 atgacgcgcc gcagagggtc aaatacgacc tggccagcgc ccggctcgcg ttgggccacg
    13561 agtccgcagc cgtcgaggcg ctcacggaag ttttgtcaat ggacccaagc gatatccggg
    13621 ccccttggct gttgtttctt gcgcggatgc aactcgcccc aacctgggat ccaatccgaa
    13681 acgacccccg cttccaggcg ctgctgaaag agtacgcggc gacggcggca tcgacgtcca
    13741 acgcgccgac ggcagccggc gcgcgcggta cgaacggctg accgccgggt tggccggcga
    13801 ggggcggctg gcggcgccga gaccgggatg cctcaaggaa ctgcaacgtc accacctgtt
    13861 ccgggtggcg gcgacccatg tagtggtcgg ctggctggtc atccaggtcg tcacggtcat
    13921 cttcccgttg ctgcagtttc ccccatggcc ggcgcgcgac atcgtgttgc tggtgctggt
    13981 gctggtcggg ttcccgatcg tgctggtgct cgcatggggg ttgcagacgc cgcgtgacga
    14041 acggggcgca tcggccagcg tgcgccggcg ttcgctcagg acgggtttca ctcgcggggg
    14101 cgtcggtgta ttgatcgcga tgctcgcagg cgcggtgtga tgtgtggtgg gtatccgggt
    14161 cccatcgaac acaccttgcg cgcgatgcgc cggcggaacc ggtggcggcc tcctcgcccc
    14221 tcggcaaccc tcgatcagtc ctgattcgag cagtgcttcc gctcgccgtg caggtcgccg
    14281 gcaccgcgag cgagtacttg ggcgagggca tcagcgaaga gttgttgaat gcgctctcgc
    14341 gcactccggg gctggaggtg atcggtcgca cgtcctcgtt ccggttcggc aacagcaccc
    14401 tcggcgccgc gaaatcgggc ggcaactcgg cgtgcgcaat ctcctctcgg gcgacgtacg
    14461 ccgttccggc aacaagctgc gcatcgacat ggaactggac gacaccgtca ccggcgggaa
    14521 gtgcgtggac cgctagactc gccaagaggc gaaacccagt tcgtcgagtc cgcagctcgc
    14581 ggtctgccgg acacatgccg gcgagaccgc gtcggcgcgc gcggctttgc aaaatctgcc
    14641 ggcggatggg gccaccgtta cgtggatcgc acccatccgg cggcgatcta tgcggcgctc
    14701 ggcgacgggg acggtgcgat ggcccagctc gaagaagcgt accgcgaccg ctccgcgcac
    14761 atggacggcg tctggatgga cccgtggtat aagccggctg cacggcgatg cgcgcttcga
    14821 ggcgtcgccc ggatagcggc aggcgctcgt cctgtcgctt cagcggcatc ccggcaggtt
    14881 ttcgcagcac cggggtcagg aggtcgtctt gagcttttgc tgcgcacgcc gcgcgtgcgc
    14941 ccgcggcgtg atcggcttgc cgtagcggca cgtgtagaac tgcgtgattt ccgcgcccag
    15001 gaacaggatc agcgcgctgt aataaaccca cactaccagc aggatcagag agccggccgc
    15061 acccgcggag ctgtagcgca ggtgggcgag ataaacgccc agcgcatact tgccgatcgt
    15121 gaacagcacc ccggtgatca gcgcgccgac gatcacgtcg cgccaatcga tgaccgcgtc
    15181 cggcacgtac ttgaacagca tcgcgatcac gccggaaatg attgccagcg acaccagcgc
    15241 atagaccacc cgcagcaccg tcccggcacc ggggaacagg ccggacaggt agccgtgcgc
    15301 acccgcgagt gcggcgctcg cagccatcga caccagcagg atgaaggcca tgcccaagac
    15361 gatggcgagc ccgagcagtc gcgagccgag cagccgccag atcgcgcttc ggctcggtac
    15421 tgccttgacg tcccagatct ggttgagcgc aatctgcagg tggccgaata cggtggtggc
    15481 gccgaacacc aggatcgcgg tgccgatgat ggtcgagatg acgctggcct gatggttgcc
    15541 gctctgcgac agcaacgcgc ggaccacatc cgcctgctgc tggcccacca tcgaaccgac
    15601 cttggcgagc agttgctcgc gcgcggcctc cctgccgaaa accagccccg ccacgcccgt
    15661 aagcaacagg aacagcggca ccatcgacaa caacgtgaaa tagctcagtg ccgccgcaag
    15721 ctggaacggc ttgtcgccga ggaattcctg cacgaacgac ttcgcgctcg acagtgggga
    15781 atgcgattgg gtcccgtctt ccatgacgtg ggcgccctgc ggtggagtcg ccgatagcat
    15841 ggcagcaatt gcgtggaatt ttcgtgcatc cggcgaggcg gttcgggtcc ggttcgggtt
    15901 ggaggtgccc gcggtcaacg cggcaacggt cccgcgccgg cggatcggtt aaaatcgcgg
    15961 ttttgcggga ggcagcatgg gcgcatcctc atccgccggc gcacgctttc gcgccgcgct
    16021 tgccgccgaa acaccgctgc aggtcgccgg cgcgatcacc gcctacgccg gcctgatggc
    16081 caggcgggtg ggctaccgcg ccttgtacct ctcgggcggt ggcgtcgccg ccaattcgct
    16141 cggcatgccc gacctcggca tttcgaccat ggaagacgtg ctgaccgacg cgcgccggat
    16201 cgtggatgcg acgcggttgc cgttgctcgt ggacatcgat accggttggg gcggagcctt
    16261 caacatcgcg cgaaccatcc gctccttcga gcgcatcggc gttgccgcgg tgcacatgga
    16321 ggaccaggtc ggacagaagc gttgcggtca tcgtcccggc aaggaagtgg tcccgacggg
    16381 ggaaatggtc gatcgcgtga aggcggcggt ggacgcgaaa atcgatcgtg atttcgttct
    16441 catggcgcgc accgatgccg ccgcggtcga aggcatcgat gccgcgatcg agcgtgcggt
    16501 ggcctacgtc gaggccggcg cggacatgat tttcccggaa gccatgaaga cgctggacga
    16561 ctaccgcaag ttcagggccg cggtgaaggt gccgatcctc gccaacctca ccgagttcgg
    16621 cgccacgccg ttcttcaccg tcgacgaact gcgttcggcc aacgtggaca tcgcgttgta
    16681 ctgttgcggc gcctatcgcg cgatgaacaa ggccgcgctc ggtttctacg agaccgtgcg
    16741 ccgcgaaggc acccagaaat ccgtggtgcc cgcgatgcag acgcgcgagg aactgtacga
    16801 tttcctcggc taccacgcct acgaaaagaa gctcgacgaa ctgttcgcgg gcggcagcaa
    16861 ggactgatcg ggcatgtcgg actcgacgcc gccccaggac gatccgcagc acgaaagtgc
    16921 gaggcgtggc gtccgctggg atgccctggc cgcgatcatc gcgtcgttgg tcggcctgct
    16981 tgcattgatc gtcgcgggct acaccgccta catcgaacgc cagcaggtgc gcgcgcaggt
    17041 atggccgtat ctgcaagcgg gatacagcgg tacgttccat cgctacatct ggatcaacag
    17101 gggcgtggga cctgcggtcg tgaagagcgt gcaggtgctg gtcgatggca agccgcagca
    17161 gaattggaaa acggtgcagc aggcgctccg gatcccctcg ttccagtatc accaatcggg
    17221 tttgagtggc accgtggttt cgcctggcca gaccctccag tggctccagt tcgaggaaaa
    17281 agacgaatac gaagatttcc gtcgcgcggc cgagcgggcc agcctgcaga ccaaggtttg
    17341 ctattgctcg acgctcggcg attgctggat gaatgactcg gcaaaaaagg acccgcgggt
    17401 gagcgtcgag caatgccccg cgcttccgga atcgcaacag ttcgacgatt gaacccgcat
    17461 gtccacgccg ccttccaact ccgatcaccc ggtcccacgg cagcgcaggc gccgtgaggt
    17521 cgagtgggac gcggttgcgg cgatcatcgc gtcactggtg ggctttctcg ccttgcttgt
    17581 cgccgggtac accgcttacg tcgagcgcta caccgcggaa atccagaaca agcaggtgca
    17641 ggcgcaggta tggccgtggc tggaggccgg caacaacgac gacacgggtt ccatcgaggt
    17701 cgtcaacaag ggcgtgggtc ccgccatcgt gcgcagcgcc cagatcttcg tggacgggaa
    17761 gccgcagcgg gattggcagc acgtgctggc ggccctgggc gcggcgcagc atggattttc
    17821 gcaaatgacg ctcaatccga acgttctggc gcccggcgag aaggtggccg cgatccggtt
    17881 caagaacaag gatgactacg cgcggttcag ggctgccgcc gtcgcgcacc tcacgttgat
    17941 caacatctgc tactgctcga gcctgggcga ctgctggcgg tacagcgacc agcacctggt
    18001 cggatacaaa tcgctggcca agctggtgac gccggttgcg caatgcccga agttgccgcg
    18061 cgacgccgtt ttcatcaatt gagtaccact cgtacggatt tgcaacatac ggagagacat
    18121 ccatgagcga caatgcacaa gttcttccga aggccaagaa gtcggtggcg ctttccggcg
    18181 tcgccgccgg caacaccgcc ttgtgcaccg tcggccgcag cggcaacgat ttgcactacc
    18241 gcggctacga catcaaggac ctggcgaccc gcgccacctt cgaggaggtt gcctacctcc
    18301 tggtgcacgg ccatttgccg acctgggcgg aattgaacgc ctatcgtgcg aaactgaagc
    18361 gcctgcgcgg cctgccgcag ccggtcaggg cggcgctcga actgctgccg gcgtccaccc
    18421 atccgatgga cgtgatgcgc accggatgtt cggtgctcgg caccgtgctg cccgaagccc
    18481 ccgaccacaa cgtcatcggt gcccgcgaca ttgccgacca cttgatggcg agcttcggtt
    18541 cgatgttgct gtactggtac cactattcgc acaatggccg tcgcatcgaa gtcgaaaccg
    18601 atgacgagtc tcttgcgggg cacttcctgc acctgctcca tggcagggcg cccggtgaac
    18661 tccatgccaa tgcgctggac aagtcgctgg tgctgtacgc cgagcacgaa ttcaatgcca
    18721 gcaccttcac cgcgcgcgtg atcgccggga ccggctcgga caggtactcg gcaatcaccg
    18781 gtgcggtcgg cgcgttgcgc ggtcccaagc acggcggcgc caacgaggtc gcgatggaaa
    18841 tcatctcgcg ctaccggacg ggcgacgagg ccgatgccga catccgcgcg cgggtggagc
    18901 gcaaggaaat catcatcggc ttcggccatc cggtgtacac cgtgtcggat ccgcgcaacg
    18961 agatcatcaa ggaaatctcg cgcaagctgt gcaccgaagg cggcaatccg cgcctgttcg
    19021 aggtatccga gcgcatcgag cgggtgatgg gcgaggtcaa gaagatgttc ccgaacctcg
    19081 actggtactc ggcgagcgtc taccacatgc tgggcgtgcc gaccgcgatg ttcacgccgc
    19141 tgttcgtgat cgcgcgcacc tcgggctgga gtgcgcacgt catcgagcag cgccaggacg
    19201 gcaagatcat ccgcccgagc gcgaactaca cgggaccgga ggaccaggcg tacgtgccga
    19261 tggaaaagcg ctgagaacaa tcgagcgcag gccgggcgga gaggtgtttg acgcgtcggc
    19321 cgaaaaccca ccccaacgaa gccgctcttg gaggttggcg ctgagcgcag cgaagcccaa
    19381 tgcgttcgcc attgttgggc ttctcccgga ccagtccggg atcagcccaa cctacaagag
    19441 ccggtgctga ccgcagtcct ccaagtaggg gtcaaaaccg cacagatcgc gcgttgatcg
    19501 acctttgcgt agaggcaagg ttcgccgatg cccgcgttga cgcagcgatc acgccgcctg
    19561 gtcgagccgc tcgcccttca gcgcgttctg caccgccgga tccgcgcgca tccgttgctc
    19621 gaacttcttc acgttctcca ggccggaaag gtcgaccttg acggcccgcg accagcgcac
    19681 cagcacgaac agatacgggt cggcgatcga acgcgtcccg gccagccagt cgtgcttgcc
    19741 gagttgcgcg tccaccaact cgaaataccc gcgcagccgc ttgcgggcgt tttccttggt
    19801 cttctcgatc gcggccgcgt caccaaggaa cgccgtcgtg ccgaaaagcg gcttgaacgc
    19861 gggatgcatg tcggagttga tcaacgccag ccagcggttc acttcggcgc ggccgcgcgg
    19921 ggtgtcgtcg ccgccgagct tcgcgtccgg atacttgtcg gccaggtaat tgaggatcgc
    19981 cgcgttctcg aaaaggatcc agccgtcgtc ctcttccagc gtcgggacct gcccgttggg
    20041 attgagccgg cgatatgccg gcctggagag ctcctccttc tgcatgagct gcaccgtgta
    20101 cggcttcccg atccattcca ggacgatgtg gctggcgagc gcgcaggaac cggagaaact
    20161 gtagagcttc atcgaagcct ccttgtggca ttcgggggat cgcgacggcg cctcgtggcc
    20221 ggggcggcgc gtgtacggaa gtggaaacgc accaactgct cttgttgggc acgcgt
//
LOCUS       DASSNH010000096        18548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_135569, whole genome shotgun sequence.
ACCESSION   DASSNH010000096 DASSNH010000000
VERSION     DASSNH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 18548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18548
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135569"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VFK00_03435"
     CDS             <1..808
                     /locus_tag="VFK00_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7592039.1"
                     /translation="HEVVGIVEQVGEGVEAVAPGAHVGIPWLGGTCGECAYCRAGREN
                     LCDFPEFTGYTRPGGFASHVVARAAYCIRLPDDVDAVATAPLLCAGLIGWRCLQRAGD
                     ALRVGLYGFGAAAHIIAQVAVHQGREVFAFTRPGDTVAQSFALSLDCAWAGGSDVSPP
                     RQLDAAIIFAPVGSLVPAALKAVRKGGRVVCGGIHMSDIPAFPYRLLWEEREVVSVAN
                     LTREDARGFFSTAACLGIKTTTKSYPLERANDALADLRNGGFSGAAVLVP"
     gene            1222..1887
                     /locus_tag="VFK00_03440"
     CDS             1222..1887
                     /locus_tag="VFK00_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7592040.1"
                     /translation="MNLLLIEDSERLRATLARGLRAEGYVVDVAGDGVEASAFLKAYT
                     YDFLVLDLGLPRLDGYGVLRSLPAGGERPRVLVLSARDRVRDRVAALNAGADDYLVKP
                     FAFDEVLARLRALGRRPSEAKADVLEHAGVRLEPQARRARANGEVLNLTPREFALLEL
                     LLRHRGRVFTRAAILERIAGSDSEASDRGIEVVVFGVRRKLSAAGCGELIENRRGAGY
                     LIP"
     gene            1884..3302
                     /locus_tag="VFK00_03445"
     CDS             1884..3302
                     /locus_tag="VFK00_03445"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7592041.1"
                     /translation="MNSRMAGNARKARMQTVSLRARLTALLAIAVLVALGAAAWIVDW
                     RADSEMQQRFDASLLARAQALAALARLENGRVVLDAREGAPGVLPSESGASWYALECG
                     GVAVSQTPVAPPPITVAGDPAFVDARLGNRTVLRVVGLRFAPTTGNRRVATGAPACTL
                     RYGGDRGPLDDILATLDFILLGSLLGACVVVMLATPWLVRRGLRPIAVLDHAMAGIGP
                     DTPGARLPQSGTTELVPLVARFNEVLGRMDAGLARERQFASGLAHEFRTRLAELRALV
                     EVETRYPSGRDTRELLAEAGSIGAELEAIVGALLQLTRIQSGLEETRPERVLLQPLLA
                     RVHERHQEAALARGMRIDSHVAAEPGFAIDTDPTLLEIVLDNLLGNAVAYAPGGSVVS
                     LGADRESVTVGNAAPSLRPDDLAQFGQRFWRKGMPGAGHAGLGLALAGAAARALQMRL
                     AFELHGGVLQASVRWNPAPANE"
     gene            complement(3422..4066)
                     /locus_tag="VFK00_03450"
     CDS             complement(3422..4066)
                     /locus_tag="VFK00_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7592042.1"
                     /translation="MKACTHMRRLTTLFLVCICALAPMRAARAASGPFGIDHRIAYDN
                     SGIWARHNQKVLVGATIVTIVGGSFALGDQDKLGDTFWRSLDSMAVSSAVSQVAKWTF
                     QRERPSQTSDPNEFFKGIRYESFPSGEVTAIAGAVTPFVVAYGHDHPAVYALELLPLY
                     DAAGRVKTRGHWQSDVLAGWAIGTAAGIWAEHRNAPLILGWLPGGFEVGFIHRF"
     gene            complement(4279..5862)
                     /locus_tag="VFK00_03455"
     CDS             complement(4279..5862)
                     /locus_tag="VFK00_03455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7592043.1"
                     /translation="MPRWAATPVAARRLGIACWCALLLPLAILLPPIPIDETRYLTAA
                     WEMFNYGQWLVPTVNGAWYSDKSPLLFWLIAGGWKVVGVHTWVARVETLAIAVAIVVT
                     LRRVAQRLGMDARGAETAMWLLAGCAGFAVFATAIMFDLLLTLCVLAALHGLLDLDRG
                     KWKRGVGVLGTALGLGILAKGPVMLLHVLPVAVLAPAWSGAARAHMARWYVALATGLL
                     IGIAIALAWLLPAAWYAGPGYWQPLVDKVSGRLARSFAHSRPFWWYLPLVPLLLLPWL
                     LALRAPRAAWAVVWRGRFGRFATCWWVVPFVGFSAISGKQIHYLLPLLPAWALMGAWL
                     LQQRGARLRPTLFVVLALAVAAALAWLPFRAASTFGWTARPALAWFALVGIVVAAGAW
                     RAWGHDPKGLALSATALAATAMLAGALAFRPALDVAPEAAFVKQELVAHVPITHVGWH
                     NGLFAYTGRLRQPLPWIPHAAVRAWCRTHPDGILLTSNSHLAPPGISPYRAWPYALSG
                     DHRIAAWRAADVLATGASQ"
     gene            complement(5865..6983)
                     /locus_tag="VFK00_03460"
     CDS             complement(5865..6983)
                     /locus_tag="VFK00_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7592044.1"
                     /translation="MFSYDPLVVRFGTLEDMVLLTPLLRLLHRRWRRPCRVMGSGRWL
                     EPLLDGRPDVQAVLTVSNLERPYWFDGTQHDAVQRLRMQPPGAVYVCDDPATVKVRRL
                     LRHAGVLADQCRFANPDCPLRDGEHWIDRWQRFAEMTPPAFVPGNFRADARGPAKPWL
                     VSNAGDRADLAEWLDRRGLADAKIILLQPGSGRARSRGGDDSSADAECWPSANWVALI
                     HALLADPGDFSVIVCGAPPDTALPRDLASSVGSPRVQVVGRDLPLRRFMALCGHAHAM
                     ISVDSGRAQVAAASGCPLIVLYGSRQPARWCPRSASGSPVLHLGGPPARSRVDEVTLD
                     EVLAAWRALRGSSPASASPERPGPRRGCSGQPVRNVPH"
     gene            complement(7287..7994)
                     /gene="phoU"
                     /locus_tag="VFK00_03465"
     CDS             complement(7287..7994)
                     /gene="phoU"
                     /locus_tag="VFK00_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7592045.1"
                     /translation="MPITPHDHLVKSYDDEMSRLTTEIAAMGELSARQLEAAMDALHE
                     RDGEVAQSVADNDDAIDEQERQISSDVLRLLALRQPMARDLRDVLAALRIASDIERIG
                     DHAENVARRSIALNQSEPVPLASGLTALATAAAGMLREVLRAYTARDAQAARAAWARD
                     AELDALYTGLFRELLTYMMEDPRSITACTHLLFIAKNLERIGDHATNIAEEVWFAVRG
                     ESLKEARTERKRRADEA"
     gene            complement(8043..8849)
                     /gene="pstB"
                     /locus_tag="VFK00_03470"
     CDS             complement(8043..8849)
                     /gene="pstB"
                     /locus_tag="VFK00_03470"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET7592046.1"
                     /translation="MPSAPSSLAAVASAAVPPRPKLAVRELDFFYGDFRALKDISMDI
                     PEKKVTAIIGPSGCGKSTLLRVFDRIYAVYPGLRASGEVLLDGENILDSKYPLHRLRS
                     KVGMVFQKAVPFPMTIFENVAWGIRHHERLSKPDMAKRVEQALRRAALWDEVKDKLKR
                     SALGLSGGQQQRLCIARAIALRPDVLLLDEPTSALDPIATAKIEQLIEELKNDYTLVI
                     VTHNMQQAARVSDRTAFMYLGELVEFDDTERIFTNPGERQTEDYISGRFG"
     gene            complement(8875..9738)
                     /gene="pstA"
                     /locus_tag="VFK00_03475"
     CDS             complement(8875..9738)
                     /gene="pstA"
                     /locus_tag="VFK00_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7592047.1"
                     /translation="MDIHVRRRAVNAVAMALSGFAAAVGLAYLVWILWVTLKNGLGAI
                     DLELFTKITAYGAEGGLSNAIVGSLVMDMIALAICAPIGVLAGTFLAEYANRTRLGAI
                     IRFLNDILLSAPSIVLGLFVYMVVVLPMSNLTDGAVSFSGLAGAIALALIGLPVIVRT
                     TDEMLQLVPGTLREAALSLGVAQWKLTVQVLMRAATSGIVTGVLLALARLAGETAPLL
                     FTAFGNNFMAFHPLDKMGSVPVAIYQYTNDPNPALHAIAWAGALLITLFVLLLSLSAR
                     WYLHRHKVIHD"
     gene            complement(9744..10715)
                     /gene="pstC"
                     /locus_tag="VFK00_03480"
     CDS             complement(9744..10715)
                     /gene="pstC"
                     /locus_tag="VFK00_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7592048.1"
                     /translation="MSAIHVPIANQNAARRSRADVRHDRWFRWLLKACALLVLAALLG
                     AALSTLWGGRDVLFGHGLHFLTSDAWDPVTNDYGAWAPIFGTLVTSVIALILAVPTAF
                     GIALFLTEIAPTWLRGPVATAIELLASIPSIIYGMWGLFVFVPFMARIEPGLSDTLGR
                     IPMLGKLFQGPPIGLGLLTAGIVLAVMILPFICSVMREVFQTVPNRLKESSYALGSTT
                     WEVAWDIVLPHTRSAVIGGILLGLGRALGETMAVTFVLGNAYDITTSLLMPGTSISSS
                     IANEFNEAIGMHRSALVALGFLLFVVTFVVLLVSRLMLRRLARREGA"
     gene            complement(10856..11875)
                     /gene="pstS"
                     /locus_tag="VFK00_03485"
     CDS             complement(10856..11875)
                     /gene="pstS"
                     /locus_tag="VFK00_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7592049.1"
                     /translation="MHRNKLRLLALAMSLALGIGAAHATDVTGAGSSFVYPVISKWSA
                     DYAEKTGNRINYQSIGSGGGIAQIKAGTVDFGASDKPLDEATLDKLGLGQFPDVIGGI
                     VPGFNLQGVAPGKLVLDGAALADIFLGKVTHWDDPAIARLNPGLKLPDAKITVVHRSD
                     GSGTTFNFTNYLSKVSPEWKSKVGEGTAVNWPDGIGGKGNEGVSAYIQRIPNSIGYVE
                     YAYAVQNKLGYARMRNAAGQVVKPDAASFQAAAATADWPHAKNFDLLMTNAPGAKAWP
                     ITATSWVIMYKQPKNAARWKVALDFFRHAYTDGKSAAAALDYVPLPDSLVEQIEAYWK
                     AEFRQ"
     gene            12134..13321
                     /locus_tag="VFK00_03490"
     CDS             12134..13321
                     /locus_tag="VFK00_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7592050.1"
                     /translation="MRTKMFAVAIATGLTVSSFGAMAQSTSTDAQQLADLKAQLAALQ
                     AKVEALQERTDAQSGIHVSTQQNIEALQQVPTKSASSWADNTTVGGTMFVDFSNIDQA
                     SNGSKTDAAGTGLDVKRFYLSIDHQFNDVWSADLTTDFNYVSNDGETNLFVKKAYVQG
                     AFSKLATLRIGSAGTPWIPFVEDFYGYRFVENTLVDRMKFGNSADWGLHLLGDSGTFD
                     YQVSMVNGGGYKHPNRSDSVDFEARVGFTPIKGFAVAIGGYTGHLGEDTTTSPALHDA
                     RRYDAMVAWNANGLRLGAEWFRANDWNNVATPFSDAADGYSLWGSYDFSAASVFARYD
                     RVEPSKHLDPDLEDTYYNFGVAFPITTGIRVAVAYKNERLRNGMSGDVESREVGAWGE
                     VKW"
     gene            complement(13469..14512)
                     /gene="phoR"
                     /locus_tag="VFK00_03495"
     CDS             complement(13469..14512)
                     /gene="phoR"
                     /locus_tag="VFK00_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HET7592051.1"
                     /translation="MNTDRARVRRLATQLRDLRTVACALSDALVLLDADSRVCWCNPA
                     AAQLLGIEWPRDRGRSAAERFGEGEAGAWLRVGQGEGDDIASPVDAAVRLQVSLIPYG
                     EHRHLLIARDISRVTRLEQVRRDFVANVSHELRTPLTVIHGYLELLDPEDVPALATVL
                     NEMRAQSQRMRQIVEDLLELSRLEMQQHVPEERVEMAPMLETLAHEAKALSQDRHRIV
                     LEDGAGIDLLGSPRDLHSAFSNLVSNAVRYTPDGGTITIRWRRTPAGAEYSVIDTGYG
                     IPAEHLSRLTERFYRVSSSRSRATGGTGLGLSIVKHVLNLHDAQLRVESESGKGSTFT
                     CVFGKARLLRSGK"
     gene            complement(14509..15198)
                     /gene="phoB"
                     /locus_tag="VFK00_03500"
     CDS             complement(14509..15198)
                     /gene="phoB"
                     /locus_tag="VFK00_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002806631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HET7592052.1"
                     /translation="MHKRILVVEDEASIRDMVAFALRKADMEVIHAADARAALVAISD
                     SPPDLILLDWMLPGMTGLELARRLRAEERTAEVPIIMLTARGEEMDRVSGLEAGVDDY
                     VVKPFSTRELIARIKAVLRRTQADGGTGSIEIGGLRIDGPAHRVYAGDAQIEIGPTEY
                     RLLHFFMSHAERAYSRAQLLDQVWGASVYVEERTVDVHIRRLRKTLEPFGLDTLIQTV
                     RGTGYRFSASP"
     gene            complement(15880..15954)
                     /locus_tag="VFK00_03505"
     tRNA            complement(15880..15954)
                     /locus_tag="VFK00_03505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15920..15922),aa:Val,seq:cac)
     gene            16122..16442
                     /locus_tag="VFK00_03510"
     CDS             16122..16442
                     /locus_tag="VFK00_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HET7592053.1"
                     /translation="MEHIVHALVNYEITPWKAVGFLGAVMFASRWFVQFYATRKHKRV
                     TMPLSFWMLSICGSVLVLAYFIFGKNDSVGIVQNLFPAFVAVYNLVVHLRHPGSGTPG
                     DAPA"
     gene            16577..16870
                     /locus_tag="VFK00_03515"
     CDS             16577..16870
                     /locus_tag="VFK00_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592054.1"
                     /translation="MNAQVGTQGGTNFAPRDVAPVSLGDWILTLIILAIPIVNIVMLF
                     VWGFSSGTNPSKQNYCRAALIMTAVLVVLWVLFAATFGAALFHMARSMGGASL"
     gene            complement(16965..>18548)
                     /locus_tag="VFK00_03520"
     CDS             complement(16965..>18548)
                     /locus_tag="VFK00_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET7592055.1"
                     /translation="VVGDLFGAGKMFLPQVVKSARVMKKAVAYLLPFMEEEKKKSGDT
                     QKSNGKIVMATVKGDVHDIGKNIVGVVLACNNFEVVDLGVMVPAQKILDTAIAEHADM
                     IGVSGLITPSLEEMAHVAKEMKRQNFRIPLLIGGATTSRAHTAIKIEPNYETACVWVK
                     DASRAVGVAQSLVSKELVDNFLTKVRNEYAEVRERHRNRGPAKRLVSLKDARANGSKF
                     DWKNYTPPVPNKPGITVIGDIPIRELSKVTDWTPFFQAWELHGHYPAILGDPVVGPQA
                     TELFDDAQKILKQIIDQKWLRAKAVIGFWPVSRTGDDLELAHGDETVMLHHLRQQADK
                     PVERPNLCLADFIAPKEAGIQDWIGGFAVTAGLGIEEHLERFRKENDDYSAIILKALA
                     DRMAEALAEWMHREVRTNYWGYAPHEDLSNEDLIAEKWRGIRPAPGYPACPDHTEKAT
                     LFRLLDATNNANIELTEGFSMYPAAAVSGWYFSHPDSQYFVVGKLTKDQVEDYAKRKG
                     WTLAEAERWLSANLDYDPD"
BASE COUNT         3266 a         6009 c         6038 g         3235 t
ORIGIN      
        1 gcacgaagtc gtcgggatcg tggagcaggt gggggaaggc gtggaggctg tcgcgccggg
       61 agcgcacgtc ggcattccgt ggctcggcgg cacctgcggt gaatgcgcat actgcagggc
      121 cgggcgcgag aatctatgcg actttccgga attcaccgga tacacgcgac ccgggggatt
      181 cgccagccat gtggtcgcgc gtgccgccta ttgcatccgt ttgcccgatg acgtggatgc
      241 tgtcgccacg gcgccactgt tgtgcgcggg tctcatcggt tggcgatgcc ttcagcgcgc
      301 cggcgatgct ttgcgtgtcg gactctacgg attcggcgct gcagcccaca tcatcgccca
      361 ggtggcggtt caccaggggc gcgaggtgtt cgccttcacc cgaccgggag atactgttgc
      421 gcagtcgttc gcgttgtcgc ttgattgtgc atgggcgggc ggctcggacg tatctccgcc
      481 aaggcaactg gacgcggcaa tcatcttcgc gccggttggg tcgcttgttc ccgccgcatt
      541 gaaggcggtc cgcaaaggcg gacgggtggt atgcggcggg atccacatgt cggacattcc
      601 cgcttttccg tatcgcctgt tgtgggagga gcgggaggtc gtttccgtcg cgaacctcac
      661 tcgcgaggac gcacgtgggt tcttctccac cgcagcgtgc ttgggcatca agaccaccac
      721 caaatcttat ccgctggagc gggcgaacga cgcgctggcg gatctgcgta atggcgggtt
      781 cagtggcgcc gccgtgctgg tgccgtgatt ttcggcggct tcggtgaaga cgcgcgattc
      841 cgtggcggca gcaccaatgg tatggctctc cagcagccga cgcgcggtcg aacaccatta
      901 ggccggattt ttcattgact acccgatagc atggtcagct tgctaacgtt gtgatggcgg
      961 gattcgcccg gacgcagaac tcggaacgac tcccgcgagc ggccgctttc gtcgatccac
     1021 tggcgggccc gattcatcat cgttggtcat cagtgctccc atccggcgcg agtctggaag
     1081 atccacgtcc aagcacgttc acgcgcgctg ccaagcttgt tgattcttgc gaactccatc
     1141 gcgatccatc ggattgcaat attctccagg ctcggtgcga gaccaaatcg cctttttgtc
     1201 cccagcaagt cgatagtcgg cgtgaacctg ctgctcatcg aggattccga acgcctgcgc
     1261 gcgaccttgg cgcgagggtt gcgcgcggaa ggttacgtgg tcgacgtggc aggcgacggc
     1321 gtcgaggcat ccgccttcct gaaggcctac acgtatgatt tcctcgtgct cgacctcggg
     1381 ctgccgcgtc tggacggata cggcgtcctg cgctccctgc cggcgggggg cgaacgcccc
     1441 cgcgtgctgg tgctctcggc gcgcgaccgg gtgcgggacc gcgtcgcggc cttgaatgcg
     1501 ggagccgacg attacctggt caagccgttt gcgttcgacg aagtgctggc acggctgcgc
     1561 gcgctgggaa ggcgcccctc cgaggcgaag gccgacgtgc tggaacacgc gggcgtacgg
     1621 ctcgagccgc aggcacgccg cgcccgggcg aatggcgagg tgttgaacct gaccccacgc
     1681 gagttcgcac tgctggagct gctgttgcgt catcggggtc gcgtgttcac gcgcgcggcc
     1741 atcctggagc gcatcgcggg cagcgacagt gaagcgtccg accgcggcat cgaagtcgtg
     1801 gtgttcgggg tgcgccgcaa gctctccgct gctggttgcg gtgagctcat cgagaaccgc
     1861 cgcggcgccg gctaccttat tccatgaatt cccggatggc cggcaacgca cgcaaggccc
     1921 gcatgcagac cgtttcgcta cgcgcgcgac tgacggctct gctcgcgatc gccgtgctgg
     1981 tggcgctggg tgccgctgcc tggatcgtgg actggcgcgc cgacagcgaa atgcagcagc
     2041 gcttcgatgc ctcgctgctg gcacgcgcgc aggcgctggc ggccctggcg cggctcgaga
     2101 atggcagggt agtgctggac gcgcgcgagg gggcacccgg ggtgctaccc agcgagagcg
     2161 gcgccagttg gtatgcgctc gagtgcggcg gggtggccgt gtcgcagacg ccggtcgcgc
     2221 cgccgcccat caccgtcgcc ggcgacccgg ccttcgtcga cgcgcgcttg ggcaaccgta
     2281 cggtgttgcg cgtcgtcgga ttgcgattcg caccgacaac agggaaccgc cgcgtcgcaa
     2341 ccggtgcccc agcgtgtacg ctgcgttacg gaggtgatcg cggcccgctg gatgacatcc
     2401 tcgccaccct cgatttcatc ctgctgggca gcctgctggg cgcctgcgtg gtggtgatgc
     2461 tggcgacgcc gtggctggtg cggcgcgggc tgcgcccgat tgcggttctg gaccatgcga
     2521 tggcgggcat cggtccagat acgccgggcg cgcgcctgcc gcagagcggc accaccgagc
     2581 tggtgccgct ggtggcgcgt ttcaacgaag tgctggggcg catggatgcc ggcctcgccc
     2641 gtgagcgcca gtttgcgtct ggactggccc acgagttccg cacccggctc gcggaactgc
     2701 gcgccttggt cgaggtcgag acccgctatc cgagtggccg cgatacgcgc gagctattgg
     2761 ccgaagccgg cagcatcggc gcggaactgg aggcgatcgt cggcgcgctg ctgcaattga
     2821 cgcgcatcca gtcggggctg gaagagacgc ggcccgaacg cgtgctcctg caaccgctgc
     2881 tggcacgagt gcatgagcgc catcaggagg ctgcgctcgc gcgcgggatg cgtattgatt
     2941 cgcatgttgc cgccgaaccg gggtttgcga tcgacaccga tcccacgttg ctggagatcg
     3001 tgctcgacaa cctgctcggc aacgcggtgg cgtacgcacc gggcggcagc gtcgtctcgc
     3061 tgggcgcgga ccgcgaaagc gtgacagtag gcaacgcggc gccatcgttg cggccggacg
     3121 acctcgccca attcggacag cggttctggc gcaagggcat gccgggagcg ggtcacgcgg
     3181 gactcgggct cgcgctggcc ggcgccgcgg cgcgtgcgct gcagatgcgg ctggcgttcg
     3241 aactccatgg aggcgtactg caggcatcgg tgcgctggaa cccggcacct gccaacgagt
     3301 aggccgcgca gccaggctgt gaacccgaca ggtcgtccgc agcccaaggg caacgactga
     3361 tcggagggga gaagcccgca catgtgtgtc ggaagttctc gcgtttcgag acggcgacga
     3421 ttcagaacct atggatgaat ccgacctcga atccgccggg tagccagccg aggatcaacg
     3481 gcgcgttgcg atgttcggcc cagatgccgg ccgcggtgcc gatcgcccat ccggcaagga
     3541 cgtcgctttg ccagtgtccg cgcgtcttga cccgccccgc ggcgtcatac agcggcaaca
     3601 gttccagtgc gtacaccgcg gggtggtcat ggccgtacgc caccacgaac ggggtcaccg
     3661 cgccggcaat cgcggtgacc tcgccgctcg gaaaactctc gtagcggatg cctttgaaga
     3721 attcattcgg gtcggacgtt tgcgatggac gctcgcgctg gaacgtccat ttcgcgactt
     3781 gcgaaacggc agacgatacc gccatggaat ccagcgagcg ccagaacgtg tcgcccagct
     3841 tgtcttggtc gccgagtgcg aacgagccgc ccacgatggt cacgatcgtg gcgcccacca
     3901 acaccttctg gttgtggcgg gcccaaatgc cgctgttgtc gtaggcgatg cgatgatcga
     3961 tcccgaaggg gccgctcgcg gcccgtgccg cgcgcatcgg cgctagtgca cagatgcaga
     4021 ccaggaacaa cgtggtgagt cgccgcatgt gcgtacaggc cttcatgcct gccatccggg
     4081 agcgatgccg gatattttcg gtccccattg cttagcatct gcttgggcaa tgctttcgga
     4141 aacctttccg acgaaggcgg cacgcgtaca tcggtgatgc gcgccggtcc gcgcgtatcg
     4201 tggagcggca acggatggaa cgtggtgcag cccgccaggc aggcgcaagc cgccagcatc
     4261 gacgctcacc gccggctttc attgcgacgc ccccgtggcc agcacatcgg cagcgcgcca
     4321 tgcagcgatg cgatggtcac ccgagagggc ataaggccaa gcgcgatagg ggctgatgcc
     4381 gggtggcgcc agatggctgt tactggtcag caggatgccg tcgggatggg tgcggcacca
     4441 tgcccgcacc gctgcgtgtg gtatccacgg caacggttgc cgaagacgcc cggtgtaagc
     4501 gaacagtccg ttgtgccatc cgacgtgcgt gatcggcaca tgcgcgacga gttcctgttt
     4561 cacgaatgcg gcctcgggtg caacgtccaa agccggccgg aaggccaacg ctccggcaag
     4621 catggccgtc gctgcaagtg ccgtcgcgga cagcgccaat ccctttgggt cgtggcccca
     4681 cgcgcgccac gcgcctgccg cgacgacgat gccgaccagc gcgaaccacg ccaacgccgg
     4741 cctcgccgtc cagccgaacg tgctcgccgc ccggaacggc agccacgcca gcgcggcagc
     4801 gaccgccaac gccagcacca cgaaaagcgt cggtcgcagt cgcgcaccgc gctgctgcaa
     4861 cagccatgcg cccatcagcg cccacgcagg cagcaggggc aggagataat ggatctgctt
     4921 gccgctgatg gctgaaaagc cgacgaacgg caccacccac cagcatgtgg cgaatcgtcc
     4981 gaaccgcccg cgccacacga ccgcccacgc cgcgcgtggg gcgcgcagcg ccagcagcca
     5041 cggcaggagc agcaacggga ccagcggaag gtaccaccag aacggacggc tgtgcgcgaa
     5101 gctgcgtgca aggcgaccgc tcaccttgtc gacgagcggt tgccaatagc cgggccccgc
     5161 gtaccacgcg gccggcagca gccatgccaa ggcgatcgcg atgccgatca gcagtcccgt
     5221 cgcgagcgcg acgtaccagc gcgccatgtg cgcgcgggct gcgccgctcc acgccggtgc
     5281 cagcaccgcc acgggcagca catgcagcaa catgaccggg cccttcgcga ggatgcccag
     5341 cccgagtgcc gtgccgagca cgccgacgcc gcgcttccac ttgccgcgat caaggtccag
     5401 cagcccgtgc agggcggcga gcacgcagag cgtcagcagc aagtcgaaca tgatcgccgt
     5461 cgcgaacacg gcaaagccgg cgcagcccgc cagcagccac atcgcagtct ccgcgccgcg
     5521 tgcgtccatg cccagtcgct gcgccacgcg ccgcaacgtc accacgatcg cgaccgcaat
     5581 cgccagcgtc tcgacgcgtg ccacccacgt gtgtacgcca accactttcc agccacccgc
     5641 gatcagccag aacaagagcg gcgacttgtc cgaataccac gcgccgttga ccgtcggcac
     5701 cagccactgg ccgtaattga acatctccca tgcggcggtg aggtagcgcg tctcgtcgat
     5761 cgggatcggg ggaagcaaaa tcgcgagcgg cagcaacaag gcgcaccagc aggcgatgcc
     5821 gagccggcgc gcagcgaccg gcgtcgctgc ccagcggggc acgttcaatg cggcacgttc
     5881 cgcaccggct gcccggagca gccccgtcgc ggtcccggcc gctccggcga tgccgatgca
     5941 gggctcgacc cgcgaagcgc ccgccaagcg gcgagtactt catcgagcgt gacctcgtcg
     6001 acgcggctcc gtgccggcgg gccgcccagg tgcaagacag gactgccgga cgcactgcgc
     6061 gggcaccacc gcgccggctg gcgggagccg tacaacacga tcaacgggca acccgacgca
     6121 gccgcgacct gcgcacgccc cgaatccacc gaaatcatgg cgtgggcgtg cccgcagagc
     6181 gccatgaaac gtcgcagcgg caggtcgcgg ccgaccacct ggacgcgtgg cgatccgacg
     6241 gagctcgcca ggtcccgcgg cagcgctgta tccggcggcg caccacagac gatgacggag
     6301 aagtcgccgg gatcggcgag caaggcgtgg atcagtgcca cccagttggc agacggccag
     6361 cactcggcat cggcggaaga gtcgtcccct ccacggcttc gggcacgccc gctccccggc
     6421 tgcagcaaga tgatcttcgc gtcggccaaa ccccgccggt ccagccattc cgcgagatcc
     6481 gcccgatcgc ccgcgttcga taccaaccac ggcttggcgg ggccgcgtgc gtcggcgcgg
     6541 aaatttcccg gaacgaacgc cggcggcgtc atttccgcaa accgttgcca gcggtcgatc
     6601 cagtgttcgc cgtcccgcag cgggcaatct ggattcgcga accggcactg atccgcgagc
     6661 acgccggcat gccgcaggag ccggcgcacc ttgaccgtgg cgggatcatc acaaacgtac
     6721 accgcgcccg gcggctgcat cctcaggcgc tgcacggcat cgtgttgcgt gccgtcgaac
     6781 cagtacgggc gttcgaggtt cgagacggtg agcaccgcct gcacatcggg acggccgtcc
     6841 agcagcggct ccagccagcg gcccgacccc atcacccgac agggccgccg ccaacgccga
     6901 tgcagcaaac gcagcagggg cgtcagcagc accatgtcct ccagcgtgcc gaagcggaca
     6961 acgagcgggt cgtacgagaa catgcaccat ccgaaccttg gtcgaccgtc gcgagtgtcc
     7021 agagccaaac ttatgattcg cttagtcgat gcggcgcccg tccgggatcg tcaccccttc
     7081 gccgtcgtcc cggctgccat tgaagaccag gcggttctcg cagcggagcc cttcacaagg
     7141 gccgcagtac gaagaggggc gcgcgagaag cgggtgtcgg aggcgaaccc gcgaatggaa
     7201 gatgcccaac atctcgcccg cgctgtgaaa ggcccgccga gtacttcaac gaaggcgcgg
     7261 cgaaaagcca cacctgccag ccgtcgtcac gcttcgtctg ctcgccgctt gcgctccgta
     7321 cgagcttcct tgagagattc cccgcgcacc gcgaaccaga cttcctcggc gatgttggtg
     7381 gcatggtcgc caatgcgttc caggtttttg gcgatgaaca gcaggtgcgt gcacgccgtg
     7441 atgctgcgcg ggtcttccat catgtaagtc agcagctcgc gaaacaggcc cgtgtagagc
     7501 gcatccagtt ccgcatcgcg cgcccacgcc gcgcgcgccg cttgcgcatc gcgcgccgtg
     7561 tacgcgcgca gcacctcgcg gagcatcccg gcggcggccg tggccaatgc cgtcaagccg
     7621 cttgccaacg gcacaggttc cgactggttc aaggcgatcg aacgtctggc gacgttttcc
     7681 gcatgatccc cgatccgctc gatatcggaa gcaatgcgca gggcggcgag aacatcccgc
     7741 agatcgcgcg ccattggctg gcgcaacgcc agcaggcgca acacgtcact gctgatttgg
     7801 cgttcctgct cgtcgatcgc atcgtcgttg tcggcgaccg attgcgcgac ctcgccatcg
     7861 cgttcgtgca gcgcatccat ggcggcttcg agttgcctcg cagagagctc gcccatcgcg
     7921 gcaatctcgg tcgtcaggcg ggacatttcg tcgtcgtagc ttttcacgag gtggtcatga
     7981 ggtgtaatgg gcatagccgt gtctcgcagc caggtgaaag tggagtcgcc ggtcgcctga
     8041 tctcatccga atcttcccga gatgtagtct tcggtttgcc gctcgccggg attggtgaag
     8101 atcctttcgg tgtcatcgaa ttcgaccagc tcgcccaggt acatgaacgc ggtccgatcg
     8161 gacacgcgcg cggcctgctg catgttgtgg gtgacgatca ccagcgtgta gtcgttcttc
     8221 agttcctcga tcaattgttc gatcttcgcg gtcgcaatgg gatcgagtgc cgaggtcggt
     8281 tcgtccagca gcaacacatc cggcctcagt gcgatcgcgc gtgcgatgca caaccgctgc
     8341 tgctggcccc ccgaaaggcc gagcgcgctg cgcttcagct tgtccttgac ctcgtcccac
     8401 agcgcggcgc gacgcaacgc ctgctccacg cgctttgcca tatccggttt cgacaaccgc
     8461 tcatgatggc gaatgcccca cgcgacgttc tcgaaaatcg tcatcgggaa cggcaccgcc
     8521 ttctggaaca ccatgccaac cttgctgcgc aggcgatgca acggatattt cgagtcgagg
     8581 atgttctcgc catccagcaa cacctcgcca ctggcgcgca gcccgggata caccgcgtaa
     8641 atgcggtcga acacgcgcag cagcgtggac ttgccgcatc cggaaggacc gatgatcgcg
     8701 gtgaccttct tttccgggat gtccatgctg atgtctttca acgcgcggaa gtcgccgtag
     8761 aaaaaatcga gctcgcgtac cgcgagtttt ggccgcggtg gaacagcggc cgaggcaacc
     8821 gccgcaagac tggatggtgc gctgggcatg gtggcgtccg tgatcgtgga tggttcagtc
     8881 atggatgacc ttgtggcgat gcaggtacca gcgtgcagac aggctgagca ggagcacgaa
     8941 cagcgtgatc agcagcgcgc cggcccaggc gatggcgtgc agcgccgggt tgggatcgtt
     9001 ggtgtattgg tagatcgcca ccgggacgct tcccatcttg tcgagcggat ggaacgccat
     9061 gaagttgttg ccgaaggcag tgaacaggag cggcgcggtt tcgccggcga ggcgtgccag
     9121 cgccagcagc acgccggtga caatgcccga agtcgcggcg cgcatcagca cctgcacggt
     9181 cagcttccac tgcgcgacgc ccagcgacag cgccgcttcg cgcagcgtgc cgggcaccag
     9241 ttgcagcatt tcgtcggtgg ttcgcacgat cacgggcaaa ccgatcagcg ccagcgcgat
     9301 cgcgccggcg aggccggaga acgacacggc gccatccgtg aggttcgaca tcggcagtac
     9361 cacgaccatg tacacgaaca ggccgagcac gatcgacggc gccgacagca ggatgtcgtt
     9421 caggaaacgg atgatggcgc caagcctggt gcggttcgcg tattcggcga ggaaagtgcc
     9481 ggccaacacg ccgatcggcg cgcagattgc aagcgcgatc atgtccatca ccaggctgcc
     9541 gacaatggca ttcgagaggc caccctcggc tccatacgcg gtaatcttcg tgaacaactc
     9601 caggtcgatg gcgccgaggc cgttcttcag cgtcacccac aggatccaga cgaggtatgc
     9661 caggcccacc gcggcggcaa agccgctgag cgccatggcc accgcgttga cggcacgccg
     9721 gcgcacgtgg atgtccatgc ccatcatgcg ccctcgcgtc gcgccaaccg gcgcaacatc
     9781 aggcgcgaaa ccagcaacac cacgaacgtc accacgaaca gcaggaagcc gagcgcgacc
     9841 agtgccgatc ggtgcatgcc gatcgcctcg ttgaattcgt tcgcgatgga agaggaaatc
     9901 gaggttcccg gcatcaacag tgaagtcgtg atgtcgtacg cgttgcccag cacgaacgtc
     9961 accgccatgg tttctcccag cgcccgcccg aggccgagaa ggatgccgcc gatcaccgcc
    10021 gagcgcgtgt ggggcagcac gatgtcccac gccacttccc atgtcgtcga gcccagtgca
    10081 taggaggatt ccttgagccg gttcgggacg gtctggaaca cctcgcgcat caccgagcag
    10141 atgaacggca ggatcatcac cgccagcacg atccccgcgg tcagcagtcc gagcccgatc
    10201 ggcggcccct ggaacagctt tccgagcatc ggaattcggc ccagcgtgtc ggagagaccg
    10261 ggctcgatgc gcgccatgaa cggcacgaac acgaacaggc cccacattcc gtagatgatc
    10321 gacgggatgc tcgccagaag ctctatggcc gtagcaaccg ggccgcgcag ccaggtcggc
    10381 gcgatctcgg taaggaacag cgcgatcccg aacgccgtgg gtaccgcgag gatgagggcg
    10441 atcaccgaag tcaccagcgt gccgaagatg ggcgcccacg cgccataatc gttcgtgacc
    10501 ggatcccacg catcactggt caggaagtgc agcccgtggc cgaaaagcac gtcgcgccca
    10561 ccccacaggg tggagagcgc cgcacccagc agcgctgcga gcaccaacag cgcgcacgcc
    10621 ttcagcagcc agcggaacca tcggtcgtgg cggacgtcgg cacggctgcg gcgggcagcg
    10681 ttttggttgg cgatggggac gtggatcgct gacatggggt tttgtgaaga gccgagtctt
    10741 tgcctgctcc cttcgggaga acgtggccag gaggggcgga tgagggttcg cgcggagcgg
    10801 aatcttccgc ctgagccgaa ccctcacccc aaccctctcc cgaggggagg gggacttact
    10861 gcctgaactc ggccttccag taggcttcga tctgttccac cagactgtcc ggaagcggca
    10921 cgtagtccag ggccgctgcc gcagacttgc catcggtata ggcgtggcgg aagaaatcca
    10981 gtgcgacctt ccagcgtgcg gcgttcttcg gttgcttgta catgatgacc catgaggtgg
    11041 cggtgatcgg ccaggccttg gcgcccggcg cgttggtcat cagcaggtcg aaattcttgg
    11101 cgtgcggcca gtcggcggtg gcggcggcgg cctggaagct cgcggcatcc ggcttcacca
    11161 cctggccggc ggcattcctc atgcgcgcat agcccaactt gttctgtacg gcgtaggcgt
    11221 actccacgta accgatcgaa ttcgggatcc gctggatgta cgccgagacg ccctcgttgc
    11281 ccttgccgcc gatgccgtcc ggccagttca ccgcggtgcc ttcaccgacc ttggacttcc
    11341 actcgggact gaccttggag aggtaattgg tgaagttgaa ggtggtgccg gagccatccg
    11401 aacggtgcac cacggtgatc tttgcgtccg gaagcttcag gccagggttg agcctcgcaa
    11461 tcgcggggtc gtcccagtgt gtgaccttgc ccaggaaaat gtccgcgagc gccgctccgt
    11521 ccagcaccag cttgccgggc gccacgccct gcaggttgaa gcccggcacg atgccgccga
    11581 tcacatcggg aaactggccg aggccgagct tgtccagggt ggcttcgtcg agcggcttgt
    11641 cgctggcacc gaaatcaacg gtgccggcct tgatctgggc gatgccgccg cccgagccta
    11701 tcgactggta attgatgcgg ttgccggttt tctccgcata gtcggcggac cacttcgaga
    11761 tcaccggata cacgaagctg gacccggcgc cggtgacgtc ggtggcgtgc gccgcgccga
    11821 tgccgagcgc gagtgacatg gcgagggcaa gcaggcgaag cttattgcga tgcatggtgg
    11881 tcacgcgagg tctcctgggt gcgtcgtggt cgatggcgtc aattacaaat gccccgtgtt
    11941 gccgttcgat gagaaaaata tttcggttgg atgacacccg ggtgacccag atcaaatccg
    12001 cgatgccccc atgcagtgtc atgcacctgt cacacgccgg gaacaactcc gcaacgatcg
    12061 cggccgaaca tgtccggcaa ccccgcggac aaacgccatg cgggaatgcc ttccgacgat
    12121 aggagatgca tacgtgcgta ccaagatgtt cgcggtggca atcgccaccg gcttgacggt
    12181 ttccagtttc ggggcgatgg cccaatccac gtccaccgac gcgcagcaac tggccgacct
    12241 caaggcacaa cttgcggccc tgcaggccaa ggtcgaagcg ttgcaggaac gcaccgacgc
    12301 gcagtccggc atccatgtca gcacccagca gaacatcgag gcactgcagc aagtgccgac
    12361 caagtccgcc tcttcgtggg ccgacaacac caccgtcggc ggcacgatgt tcgtcgattt
    12421 ctccaacatc gaccaggcca gcaacggcag caaaaccgat gcggccggca cggggctcga
    12481 cgtcaagcgc ttctatcttt cgattgacca ccagttcaac gacgtctggt cggccgacct
    12541 gaccactgat ttcaattacg tcagcaatga cggcgaaacc aatctgtttg tgaagaaggc
    12601 ctacgtgcag ggcgcgttct cgaaactcgc gacattgcgc attggctcgg ccggcacacc
    12661 ctggattccg ttcgtggaag atttctacgg ctaccgattc gtcgagaaca ccctggtcga
    12721 tcgcatgaag ttcgggaatt ccgcggactg gggcctgcac ctgctgggcg acagcggcac
    12781 gttcgactac caggtgtcga tggtcaacgg cggcggctac aagcatccga accgctccga
    12841 cagcgtcgat ttcgaggctc gtgtcggctt cacgccgatc aagggcttcg cggtcgccat
    12901 cggcggctat acgggtcatc tcggcgagga caccacgact tcgcccgcgc tgcacgacgc
    12961 gcgtcgctac gacgcgatgg tcgcctggaa cgcgaacggt ttgcgcctgg gcgcggaatg
    13021 gttcagggcg aacgactgga acaatgtcgc cacgccgttc agcgacgcgg cggatggcta
    13081 ctcgctgtgg ggcagctacg acttcagtgc cgcatccgta ttcgcgcgtt acgaccgcgt
    13141 cgaaccgagc aagcatctcg atccggacct ggaagacacc tactacaact tcggcgtggc
    13201 attcccgatc accacgggca tacgcgtggc ggttgcctac aagaacgagc gcctgcgcaa
    13261 cggcatgagt ggcgacgtcg aatcgcgcga ggtcggcgcg tggggcgaag tgaagtggtg
    13321 acgaggcctg ctccggacgg actcgaggtg cgtggcggcg cgcgtcggcg agcccaagga
    13381 ttgaatacag ccatccgtgc cgctcgcgtt ggcgcacgct tcatgcttca tgtccctgcg
    13441 gatgctcgat gcgaattcgc ggtacaggtc acttcccaga ccgcaacagc cgcgctttac
    13501 cgaatacaca tgtgaatgtc gaacccttgc cggattcgct ttcgacccgc agctgcgcgt
    13561 cgtgcagatt cagcacgtgc ttgacgatcg aaagtccgag gccggtaccg ccggttgcgc
    13621 gcgagcggct ggacgagacg cggtagaagc gttcggtgag tctggagagg tgctcggcgg
    13681 gaatgccgta gccggtgtcg atcaccgagt attctgcgcc ggccggcgtg cgtcgccagc
    13741 ggatcgtgat ggtgccgccg tccggtgtgt agcgcaccgc attgctgacc aggttggaaa
    13801 atgcactgtg caggtccctg ggcgaaccca gcaggtcgat cccggctccg tcttccagca
    13861 cgatccggtg ccggtcctgg ctcagggcct tcgcttcgtg agccaaggtt tccagcatcg
    13921 gcgccatctc gactcgctcc tcggggacgt gctgctgcat ttccagtcgc gacagttcga
    13981 gcaagtcctc gacgatctgg cgcatgcgct gcgactgggc ccgcatctcg ttcagcacgg
    14041 ttgccagcgc cggcacgtcc tccggatcga gtaattccag gtaaccgtgg atcacggtca
    14101 gcggcgtgcg cagttcgtgc gatacgttgg cgacgaaatc gcggcgcacc tgttcgagcc
    14161 gggtgacccg gctgatgtcg cgcgcaatca gcaggtggcg atgctcgccg tacggaatca
    14221 gcgagacttg cagccgcacg gcggcatcga cgggcgaagc aatatcgtca ccctcacctt
    14281 gtcctacccg caaccacgcg ccggcctcgc cttcaccgaa acgttcggcc gcggaccggc
    14341 cgcgatcgcg gggccattcg attccaagca gttgcgccgc cgcgggattg caccagcaca
    14401 cccgggaatc ggcatccagc agcaccagcg cgtccgacaa ggcacacgcg accgtgcgca
    14461 ggtcgcgcag ttgcgtcgcg agacgccgca cacgcgcgcg atcggtgttc atggcgaagc
    14521 cgagaatcgg taaccggtac cgcgcacggt ctggatcaag gtgtctagtc cgaacggctc
    14581 cagcgtcttg cgcaggcggc ggatgtgcac gtccacggtg cgctcctcca cgtacacgct
    14641 tgcgccccat acctggtcca gcaactgcgc gcgcgaatag gcgcgttcgg cgtggctcat
    14701 gaagaagtgc aacaggcggt attcggtggg gcctatctcg atctgcgcat cacccgcata
    14761 cacccgatgc gcgggaccgt cgatgcgcaa accaccgatc tcgatcgaac ccgtgccgcc
    14821 gtcggcctgg gtacgccgca gtaccgcctt gatcctcgcg atcaactcgc gcgtcgagaa
    14881 tggtttcacc acataatcgt ccacgccggc ttccaggccg cttaccctgt ccatttcctc
    14941 gccgcgcgcc gtcagcatga tgatcggcac ctcggcggtg cgctcctcgg cacgcaaacg
    15001 ccgcgccagt tccagaccgg tcatgccggg cagcatccag tccagcagga tcagatcggg
    15061 cggggagtca ctgatcgcga ccagcgccgc ccgagcgtcc gctgcatgga tcacttccat
    15121 gtcggccttg cgcagggcaa acgcgaccat gtcgcggatc gaagcttcgt cttcgacgac
    15181 cagaatgcgc ttgtgcatgc gggatggccg gcacggtgac gcgactattg cagcgttcca
    15241 ttatgacagt ctcgtgacaa gggtctcgac tgcaaatctc gggggcaatg gggctgaagc
    15301 agcgtcatcc ttgcaggagc aaagcgaaag ttcggacgta ccgccttgaa tgtcagctga
    15361 gggtcgcctg cggccgacag gaaaattccc acatctccga tcggtgctca cctatatccg
    15421 tggaaattcg ggacggtgac cattgtcgcg ccggttgtcc cggcacgcgg atggacccac
    15481 gaagccgact gcacactggc cagcgccgcc gcataggcgg tgcttccatt tcgagaggat
    15541 gcaaaaatcc atgtttctcg acttgggggt acgcttggaa atcttcgttt tcctgatacc
    15601 tgcgtcattt gtccccgcgc gacgttgggc gactggctac ttcggatcgc aagttgcctg
    15661 gcgcagcttg ggcgtccggg cagagttggc gcggcgaaac gtcaactgcg gaggctgcag
    15721 gggaacaacg gagacgagtg ggcggggggc aatccggggt caccaccaag acataccgcg
    15781 aatgcagcga aacagccaga atcgcgggga gatttgggga cactcggaaa ccagaaaggc
    15841 ccgattttcg agatctggcc attcgtaagc aattgaattt ggtgggcggt gcagggatcg
    15901 aacctgcgac acctgccgtg tgaaggcagt gctctaccgc tgagctaacc gcccgagttc
    15961 gtgcgcgcgc ggcgccattt cgggaatcgc cccgcgccgg gggtgggacg caagccgcga
    16021 ccgcgggaga acggggaagt ctacggtgcg ccccggtcgc ggtcaatcgg cagtggactg
    16081 gttgctacaa ttcgccgtta tccgcaaccg gacccctcgc catggaacac atcgttcacg
    16141 ccctcgtcaa ctacgagatc acgccctgga aggcggtcgg ctttctcggg gcggtgatgt
    16201 tcgcgagccg atggttcgtc cagttctacg ccacccgcaa gcacaagcgg gtaacgatgc
    16261 ccctgtcgtt ctggatgctg tcgatttgcg gaagcgtctt ggtgctggcg tatttcattt
    16321 tcgggaaaaa cgattcggtc ggtatcgtgc agaacctgtt tccggcgttc gtcgccgtct
    16381 acaacctggt tgttcacctg cggcatccgg gcagcggcac gccgggggac gcacctgcct
    16441 agctgcagtg tcgtccttgc gctaggatcg cgtcggcgtc cgaatctccg ggcgccgggg
    16501 agagtggcgc gggcgccgag gcgtggcgac ccgtttttcg agccgcaagg ctggttccat
    16561 tgcatgggga aaagacatga acgcgcaagt tggcacccag ggcggcacaa acttcgctcc
    16621 acgagacgtg gcaccggttt cgctggggga ctggatcctt acgctgataa tcctggcgat
    16681 tcccatagtg aacatcgtga tgctgttcgt gtggggattt tccagcggca cgaatcccag
    16741 caagcagaac tattgccggg ccgccctgat catgaccgcg gtactggtgg tgctctgggt
    16801 gttgttcgcg gcaacgttcg gagccgcgct gttccacatg gcccgatcga tgggcggcgc
    16861 ctcgctgtag tcccggccgc gtcacccgtg gagcgggcaa cgtcggtcgc ctgaactggc
    16921 gcggacgctg tccgctccat cttcttcggt ctgccgccag cggctcagtc cggatcgtag
    16981 tcgaggttgg ccgaaagcca gcgctcggct tcggccagtg tccagccttt gcgcttggcg
    17041 tagtcctcga cctggtcctt cgtgagcttt ccgaccacga aatactggct gtcgggatgg
    17101 ctgaaatacc agcccgaaac cgcagcggcg gggtacatcg aaaagccttc ggtgagttcg
    17161 atgtttgcat tgttcgtcgc atcgagcagc cggaagagcg tggccttctc ggtatggtcg
    17221 ggacaggcgg gatagccggg agccggacgg atgccgcgcc atttttcggc aatgaggtcc
    17281 tcgttgctca agtcctcgtg gggtgcgtag ccccagtagt tcgtgcgtac ttcgcggtgc
    17341 atccattccg cgagcgcttc ggccatgcgg tcggccagcg ccttcaagat gatcgccgag
    17401 tagtcgtcgt tctctttccg gaagcgttcg aggtgctcct cgatgccgag tccggcggtc
    17461 accgcgaaac cgccgatcca gtcctggatg cccgcttcct tcggcgcgat gaagtcggca
    17521 aggcaaaggt tgggacgttc gacgggcttg tcggcttgct ggcggaggtg atggagcatc
    17581 accgtttcgt cgccatgggc cagttccaga tcgtcgcctg tccgcgacac cggccagaac
    17641 ccgatcaccg ccttcgcgcg tagccacttc tggtcgatga tttgcttcag gatcttctgc
    17701 gcgtcatcga acagttccgt ggcctgtggg cccaccaccg gatcgccaag gatcgcgggg
    17761 taatggccgt gcagttccca cgcctggaag aacggcgtcc agtcggtgac cttcgatagt
    17821 tcgcggatcg gaatgtcgcc gatcaccgtg atgccgggct tgttcggcac gggcggcgtg
    17881 tagtttttcc aatcgaactt cgagccgttc gcgcgcgcgt ccttcagtga caccagccgc
    17941 ttggccgggc cgcggttccg atggcgctca cgcacctctg cgtattcgtt gcggaccttg
    18001 gttaggaaat tgtcgaccag ttccttgctg accagcgact gcgccacgcc gaccgcccgc
    18061 gaggcgtcct ttacccacac gcaggcggtt tcgtagttgg gttcgatctt gatcgcggtg
    18121 tgcgcgcgcg aagttgtcgc gccgccgatc agcagcggaa tcctgaagtt ctgccgcttc
    18181 atctccttgg cgacgtgcgc catttcctcg agcgacggcg tgatcagccc cgacacgccg
    18241 atcatgtcgg cgtgctcggc gatcgcggtg tcgagtatct tctgcgccgg caccattacg
    18301 ccgagatcga ccacctcgaa attgttgcaa gccagtacca cgccgacgat gttcttgccg
    18361 atgtcgtgca cgtcgccctt gacggtcgcc atcacgatct tgccgttgga cttctgggta
    18421 tcgccgctct tcttcttttc ctcttccatg aagggcagca ggtaggccac ggcctttttc
    18481 atcacgcgcg cggatttgac gacctggggc aggaacatct tgcccgcgcc gaacaggtcg
    18541 ccgaccac
//
LOCUS       DASSNH010000097         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1030003, whole genome shotgun sequence.
ACCESSION   DASSNH010000097 DASSNH010000000
VERSION     DASSNH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030003"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..777)
                     /gene="hemJ"
                     /locus_tag="VFK00_03525"
     CDS             complement(355..777)
                     /gene="hemJ"
                     /locus_tag="VFK00_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HET7592056.1"
                     /translation="MLALWLKAFHIIGVVTWFAGLFYLPRLFVYHAEASEPAVRDRLK
                     TMERRLLVMTHIGGGLALAFGIATLTAESFYLQAAWLHVKLALVLLLVIYHAALVKLW
                     RDFARDRCRWTSRTLRLFNEAPALLLLAIVILAVVKPF"
     gene            928..1266
                     /locus_tag="VFK00_03530"
     CDS             928..1266
                     /locus_tag="VFK00_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HET7592057.1"
                     /translation="METIGWEDFAKVELRVGRIIDARVFPEARKPAYVLQVDFGPEIG
                     VRKSSAQITGLYRPEELVGKLVVGVVNFPKKQIGPVMSECLVTGFHDADGEVALCVPD
                     KPVPLGSKLA"
     gene            1431..>2188
                     /locus_tag="VFK00_03535"
     CDS             1431..>2188
                     /locus_tag="VFK00_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HET7592058.1"
                     /translation="MTPDKILETIEHDGIEFVDLRFADMRGVEHHVSFPAHAIDADTL
                     EDGKMFDGSSIAGWKGINESDMVLMPDPATAFVDPFAAHKQLNIMCDVLEPSTMQAYS
                     RDPRSIARRAEAFLKSSGVADTAYFGPEPEFFIFDSVRWQNDMGRVFYEIGSEEAAWS
                     SRYKFEEGNHGHRPVVKGGYFPVPPVDSLADLRAEMCKVLESLGQAVEVHHHEVGNAG
                     QCEIGVRFNTLLHKADDLQTMKYVIRNVAHANGKT"
BASE COUNT          411 a          686 c          719 g          372 t
ORIGIN      
        1 gatggcggcg aaatgccgcc tgcagttccg catcgggatt ctgccgggcc aacagcgatg
       61 ccatgaggac tccccgctac ggcgaaacta tagcaagccg gcgcgccggc tgcgcacgat
      121 ttgagaacgg tgtctcatta ggaatggcgg ttgtggaagc cgaaatggaa cggaccgtcc
      181 tggttggacg aaccaggagc gggcacgggg aacagggaac gaggcgggga atggggatac
      241 ggcttttgtt ccctgtcacc gcttctcaaa cccatctctt cttttcccgc ttcacttcgg
      301 ggccgggtct tgcgagtcca tcgctttgcg atgcagcgat cacggatgat cgcactagaa
      361 aggcttcacg acggcgagga tcacgatcgc gagcagcagc agggccggcg cctcgttgaa
      421 caagcgcagc gtgcgcgagg tccagcggca gcggtcgcgc gcgaagtccc gccacagctt
      481 caccagggct gcgtggtaga tgacgagcag cagcaccagc gcgagcttca cgtgcagcca
      541 cgcggcctgg aggtagaacg attcggcggt gagggtggcg atcccgaacg cgagcgcgag
      601 tcctccgccg atgtgggtca tcaccagcag gcgccgctcc atcgttttga ggcgatcacg
      661 gaccgctggc tcgctggcct cggcatggta gacgaacagc cgcggcaggt agaacagccc
      721 ggcgaaccag gtcaccaccc cgatgatgtg gaacgctttc agccacaacg ccaacatggc
      781 ggtctccaac cgggggattt ttgcacgggc gtggacgccc gcatcggagg ggaaacggac
      841 gcgcgccgcc ggcgccccct gctgcgggtt gtcgccttct acgccgtgcg cgcggcggcc
      901 ggtagtatgc ctgcccggag gtggtgcatg gaaacgattg gctgggaaga cttcgccaag
      961 gtcgaactgc gggtcggccg gatcatcgat gcgcgggtgt tccccgaggc gcgcaaacct
     1021 gcctacgtgc tgcaggtcga tttcggaccg gaaatcggtg tccggaagtc gagtgcccag
     1081 atcaccggct tgtatcggcc cgaggagctc gtcggaaagc tggtggtggg cgtggtgaat
     1141 ttcccgaaga aacagattgg cccggtgatg tcggaatgcc tcgtcaccgg cttccacgat
     1201 gcggatggcg aagttgcctt gtgcgttccg gacaagccgg tgccgctggg atcgaagctt
     1261 gcctgatccg gttgcggccg atcacccgcg tttcaccatt ttggtgcaac gacggttcca
     1321 gtctggtgca tccggatgag cgggtacaat cgaacccttt gaaatccgcc acctcgccgt
     1381 tggcaccgga cgtgcaacgg ccacgcgtcc gccccgaccc agagcccaca atgacccccg
     1441 acaagatcct ggaaaccatc gagcacgacg gcatcgagtt cgtcgacctt cgcttcgccg
     1501 acatgcgcgg cgtcgaacac cacgtcagct tccccgccca cgcgatcgac gccgataccc
     1561 tcgaggatgg caagatgttc gacggctcgt cgatcgcggg ctggaagggc atcaatgaat
     1621 cggacatggt cctgatgccg gacccggcca ccgcgttcgt cgacccgttc gccgcgcaca
     1681 agcagttgaa catcatgtgc gacgtgctgg agccgtccac gatgcaggcg tattcgcgcg
     1741 atccgcgttc gatcgcgcgc cgggccgagg cgttcctgaa atcctcgggc gtcgcggaca
     1801 ccgcgtactt cggcccggaa ccggagttct tcatcttcga ttcggtccgc tggcaaaacg
     1861 acatgggccg ggtgttctac gagatcggtt ccgaagaggc cgcgtggagc tcgcgctaca
     1921 agttcgagga aggcaatcac ggccaccgcc cggtcgtgaa gggcggctat ttcccggtgc
     1981 cgccggtcga ttcgctcgcc gacctgcgcg cggaaatgtg caaggtgctg gagtcgctcg
     2041 gccaggccgt cgaggtgcac caccacgaag tcggcaacgc cggccagtgc gagatcggcg
     2101 tgcgcttcaa cacgctgctg cacaaggccg acgacctgca gaccatgaag tacgtgatcc
     2161 gcaacgtcgc ccacgccaac ggcaagac
//
LOCUS       DASSNH010000098         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_253611, whole genome shotgun sequence.
ACCESSION   DASSNH010000098 DASSNH010000000
VERSION     DASSNH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253611"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFK00_03540"
     CDS             complement(<1..789)
                     /locus_tag="VFK00_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7592059.1"
                     /translation="MKALSELIAAFRGNELKLPALFDALSARGPIPEDTYQRERALLE
                     RLLKDKDFNPQVAQALLAKLESLHVAVPAGVDPADEDVTALFAAAPRAQGEATVVYPA
                     STTTGREDVTQVLPSAETPGEGAVAGGNTQDTGGSSTGGSSSWKRIADAAQGEYATVG
                     MLLKGRFLLEREIGRGGMGVVFVARDERKVEARDRDPYVAVKVLNDEFRRHPDSLIAL
                     QRESRRSQQLAHDNIVRVYDFDKDGTVVFMTMEYIDGSDLKTLIR"
     gene            complement(845..1525)
                     /locus_tag="VFK00_03545"
     CDS             complement(845..1525)
                     /locus_tag="VFK00_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HET7592060.1"
                     /translation="MNAYGFRGAAVLAAVCALGLMAGCAQTGGTRPAGGSDVASSEAP
                     HECNPGVGAAIGAIAGSFFGKGKGHIAGAAIGAGIGAFACMAYNYHARKIRDARAVEA
                     QYRRERGALPASNTVEAYQSELVPGQAVQPGGKATMQSRITVLQGTRGANPQLQEQLT
                     LFSPEGKELTSVTKDATAVDGTGEYETNFDFNLPKGIQEGRYTMQSALYMNGQKVRTN
                     KVPMLVVA"
     gene            complement(1538..>2497)
                     /locus_tag="VFK00_03550"
     CDS             complement(1538..>2497)
                     /locus_tag="VFK00_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7592061.1"
                     /translation="VVFELLTGKHPFDKQSAEVAFKEGRKPPKVPGLTKRQYKTLCDA
                     VAFTSDRRLKSAEQLVQGLREIGWRERVGRPLLYAALLTLALVGAGWAVMNQLHARQV
                     AQVAARFEPASPQHYADEAQAVAALASLDDNERKQLILEQGDLVQRFLIGRVDAYWDP
                     ARGRRDYASARHVFDLLGRLHLFLPQLELKRVAMEKEKYDLDHPRPVATEVGAHPESE
                     PASAGTAGLAAAAAAQKAADAAAEKAAAEQAAARAQAERMQKAVAGILSDGERNYSQQ
                     KYSAAIANARAALQINPDDPGAKRLLKRAQQAQQRAMSNITIN"
BASE COUNT          370 a          896 c          764 g          467 t
ORIGIN      
        1 gcggatcagc gtcttcaggt ccgatccgtc gatgtactcc atcgtcatga agacgacggt
       61 cccgtccttg tcgaagtcgt acacccgcac gatgttgtcg tgcgccagct gctgcgacct
      121 gcgcgactcg cgctgcaacg cgatcagcga atcgggatgc cggcggaact cgtcgttcaa
      181 caccttcacc gccacatacg gatcgcggtc gcgcgcctcc accttgcgct cgtcgcgcgc
      241 cacgaacacc acgcccatcc cgccacggcc gatctcgcgc tccagcagga agcggccttt
      301 cagcagcatg cccacggtcg cgtattcgcc ttgcgcggca tccgcaatgc gcttccagct
      361 cgacgagccg cccgtcgaag acccgccggt atcctgcgta ttgccgcctg cgacggcacc
      421 ttcacccgga gtctcggcgg acggcagcac ctgggtgaca tcttcccggc ccgtcgtggt
      481 cgaagcggga tacaccaccg tggcttcgcc ttgcgcacgc ggtgccgcgg caaacagggc
      541 ggtgacgtcc tcgtcggccg gatccacccc cgcgggcacc gctacgtgca ggctttccag
      601 tttcgccagc aacgcctgcg cgacctgcgg gttgaaatcc ttgtccttga gcaggcgttc
      661 cagcagcgca cgctcgcgct ggtaggtgtc ctccggaatc ggtccgcgcg cgctcagcgc
      721 gtcgaacaac gcgggaagtt tcaactcgtt gccgcggaag gccgcgatca gttcggaaag
      781 cgctttcatg caggactctt gttcattccg ccgtcgtgcg cggaggtcgg ggacacacct
      841 ggactcaggc gaccaccagc atcggcacct tgttggtacg caccttctgg ccgttcatgt
      901 acagcgccga ttgcatcgtg tagcggcctt cctggatgcc cttcggcagg ttgaagtcga
      961 aattggtttc gtactcgccg gtgccgtcga ccgcggtcgc atccttcgtg acactggtga
     1021 gttccttgcc ttccggcgag aacagcgtca gctgttcctg caactgcggg ttggcgccgc
     1081 gggtgccctg caacacggtg atgcgcgatt gcatcgtggc cttgccgccc ggctgcaccg
     1141 cctggcccgg gaccagttcg ctctggtagg cctcgaccgt gttgctggcc gggagcgccc
     1201 cgcgttcgcg ccggtattgc gcttccaccg cgcgcgcatc cctgatcttg cgggcgtgat
     1261 agttataggc catgcaggcg aacgcgccga tgccggcacc gatagccgca ccggcgatgt
     1321 ggcccttgcc cttgccgaag aacgaaccgg cgatcgcgcc gatcgcggcg ccgacacccg
     1381 ggttgcattc gtgcggggct tcgctgcttg ccacgtcgga accgccggcg ggcctcgtcc
     1441 cgccggtctg cgcacaaccc gccatcaagc ccagcgcgca caccgcagcc aggacagctg
     1501 cgccacgaaa accgtatgcg ttcatgacca ccctccctca gttgatcgtg atgttgctca
     1561 tggcccgctg ctgtgcctgc tgggcacgct tcaggagccg ctttgcaccc ggatcatccg
     1621 ggttgatctg gagcgccgcc ctggcgttcg cgattgccgc ggaatatttc tgctgtgaat
     1681 aattgcgttc gccgtcgctc aggatgcctg cgaccgcctt ttgcatccgc tccgcctgcg
     1741 ccctggcagc cgcctgctcc gccgccgcct tctccgcggc agcgtcagcg gccttctgtg
     1801 ccgcggcagc ggccgcgagc cccgccgtgc cggcgcttgc gggctccgac tccggatgcg
     1861 cgcccacttc ggtcgcaaca ggcctcggat gatcgaggtc gtacttctcc ttttccatcg
     1921 cgacgcgctt gagttcgagc tgcggcagga acagatgcaa ccggccgagc aagtcgaaca
     1981 cgtgccgcgc ggaagcgtaa tcgcgccggc ccctggccgg atcccaatag gcatccaccc
     2041 ggccgatcag gaagcgctgc accaggtcgc cctgctcgag gatcaattgt ttgcgctcgt
     2101 tgtcgtcgag gctcgccagc gcggcgaccg cctgcgcctc gtccgcgtag tgctgcggac
     2161 ttgccggctc gaaacgcgcg gcaacctgcg ccacctgtcg cgcatgcaac tggttcatca
     2221 cggcccagcc tgcgccgaca agcgccaagg tgaggagggc cgcgtaaagc agcggacgac
     2281 cgacgcgttc gcgccagccg atctcgcgca atccctgtac cagctgttcg gcgctcttca
     2341 ggcgcctgtc ggaggtgaac gccaccgcat cgcacagggt cttgtattgg cgcttggtca
     2401 gccccggcac cttcggcggc ttgcgccctt ccttgaacgc cacctccgcg ctctgcttgt
     2461 cgaacgggtg cttgcccgtc agcaattcga acaccac
//
LOCUS       DASSNH010000099         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_546187, whole genome shotgun sequence.
ACCESSION   DASSNH010000099 DASSNH010000000
VERSION     DASSNH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546187"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VFK00_03555"
     CDS             <1..1380
                     /locus_tag="VFK00_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET7592062.1"
                     /translation="AETIEQKNGKYFYRFGKQLLPVKTRDITIRYRDDDGPMAERTFT
                     AYFTRHGPIVRSDNGKWIAEALMNRPVPALEQSWLRTKATDLASYMKVAELKANSSNN
                     TLFADDKGEIAFLTPQFIPKRDNSFDYLEPVDGSNPATAWQGMTPLEDMPNSIDPPNG
                     WVFNTNNWPYSAAGQYSPKRTDFPQYMDSFGENARGIHATLMLTGAKDFTKAKVITDA
                     FDSYLPAFARLVPILVKDYDALPASDAQKAELAGPIAVLRSWDYRWGLASIPTTLAIT
                     WGDKLWNEVSDAAIASGLDAHTTDLREIDYIAEKATPKQRLDALAATVDRLEKDFGYW
                     GVPWGEINRYQRLDDSIEPHFDDSKSSIGVPFTASRWGSLAASNAHRWPSTNKYYGRS
                     GNSFVAVVEFGPKVSARAIHVGGQSGNLDSPHFTDQAQRYVSGDLRTVYFWPEQLKGH
                     TTRVYHPGE"
     gene            1434..2855
                     /locus_tag="VFK00_03560"
     CDS             1434..2855
                     /locus_tag="VFK00_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7592063.1"
                     /translation="MKRAFVLLVALLASASVAHAEPKDWIAYKQPVIAFTHATVVDGT
                     GAAPKHDQTLVIDKGRITALGPAASTRVPANATVIDARGKTLLPGFVMMHEHLFYTTR
                     TDPGFYATPMPYSFPPLYLAGGETTIRTGGSLMPYTDLNVRDAIHSGKRIGPDIDVTG
                     PYLNGVESFTENMHTLTGPADATGTVDYWAAEGVDSFKAYDHITRAELKAAIDAAHAH
                     HDKITGHLCSVTFREAADLGIDNLEHAFFVASDFVKDKKPDECPQDVRASIASLDVDS
                     PEVTSLIRLLVEKHVALTSTLGVFETFVPGRPALPRVALDMLNPALRELYEATWKKVQ
                     AMPAPRAKMMQQEFAKATRLEKKFVEAGGVLMGGSDPTGYGGVIPGFSSKRQMELLVQ
                     ADGFAFPQAVKIMSLNAAKFQGRDEDIGSLAVGKRADIVVIDGDPAKDVTAIEHMPLV
                     FKDGVGYDTAAIFKSMQGQIGMN"
     gene            complement(2877..>3709)
                     /locus_tag="VFK00_03565"
     CDS             complement(2877..>3709)
                     /locus_tag="VFK00_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7592064.1"
                     /translation="QAEAWSRIASFIRGQGAAAGMQLAHAGRKASTDAPWRGGGPLDA
                     GQGGWTPVAPSAIPFDEGYPVPAALEASGIAKAIDDFRAAARRAREAGFEVIEIHGAH
                     GYLIHQFLSPLSNTRTDQYGGSLENRARLLREVIAAVRETWPSPMPLWVRLSATDWAK
                     GGWDIEECVQLARMLRADGVDVIDCSSGGTVPHPNVPTGPGYQVPFAARIRREAGIPT
                     STVGLITEPRQAEEILQRGDADVIEMAREFLRDPYFPRRAAKELGETVRVPEQYDRAW
                     "
BASE COUNT          705 a         1276 c         1147 g          581 t
ORIGIN      
        1 gccgaaacca tcgaacagaa gaatggcaag tatttctacc ggttcggcaa gcaactgctg
       61 ccggtcaaga cccgcgacat cacgatccgc tatcgcgacg atgacggccc gatggccgag
      121 cgcaccttca cggcgtactt cacccggcac ggcccgatcg tccgcagcga taacggcaag
      181 tggatcgcgg aagccctgat gaaccgaccc gttccggcgc tggagcagag ctggctgcgc
      241 accaaggcga cggaccttgc gagctacatg aaggtggccg agctcaaggc caattcctcc
      301 aacaacaccc tgttcgccga cgacaaaggc gagatcgcgt tcctcacccc gcagttcatt
      361 cccaagcgcg acaacagttt cgattacctc gaacctgtcg acggcagcaa tcccgccacc
      421 gcctggcagg gcatgacgcc gctcgaggac atgccgaaca gcatcgatcc acccaacggc
      481 tgggtgttca acaccaacaa ctggccctac tccgccgccg gccagtacag cccgaagcgc
      541 acggactttc cgcaatacat ggattcgttc ggcgaaaacg cgcgcggcat ccacgcgacc
      601 ctgatgctta cgggcgcgaa ggacttcacc aaggcgaaag tcattaccga cgcgttcgat
      661 tcctacctgc ccgcgttcgc ccggttggtt ccgattctgg tgaaagacta tgacgcgctg
      721 cccgcgagcg acgcgcagaa ggccgagctc gccgggccga tcgcggtgct gcggagctgg
      781 gactaccgct ggggtctcgc gtcgattccg accacgcttg ccatcacctg gggcgacaag
      841 ctctggaacg aggtgagcga cgcggcgatc gccagcgggc tcgatgcgca caccaccgac
      901 ctgcgcgaga tcgattacat cgcggaaaag gccacgccga agcagcgcct cgacgccctg
      961 gccgcgaccg tcgatcggct ggagaaggat ttcggctact ggggcgtgcc gtggggcgag
     1021 atcaatcgct accagcggct ggatgattcg atcgagccgc atttcgatga ctccaagtcc
     1081 agcatcgggg tgccattcac cgcgtcgcgc tggggttcgc tggcggcctc gaacgcccac
     1141 cgctggccca gcaccaacaa gtactacggc cgcagcggca acagcttcgt cgcggtggtc
     1201 gagttcggcc ccaaggtgag cgcgcgcgcg atccacgtcg gcggccagag cggcaacctt
     1261 gattccccgc acttcaccga ccaggcgcag cgctacgtgt cgggcgatct gcgcaccgtg
     1321 tacttctggc ccgagcagct caagggccac acgacgcggg tgtaccatcc cggcgagtga
     1381 ccagaatgcc ggccgcgcgt cgcggccggc aaccatcgcg aagggggacc gccgtgaaac
     1441 gagcgttcgt tctgctggtc gcgctgcttg catccgcatc ggtcgcgcac gcggagccga
     1501 aggactggat cgcctacaag cagccggtca tcgccttcac ccacgccact gtcgtcgatg
     1561 gcactggcgc cgcgccgaaa cacgaccaga cgctggtgat cgacaagggt cgcatcaccg
     1621 cgctcgggcc ggccgcgagc acacgggtac cagcaaacgc caccgtgata gacgcgcgcg
     1681 gcaagacatt gctgccgggc ttcgtgatga tgcacgagca tctgttctac accacgcgaa
     1741 ccgatccagg cttctacgcc acgccaatgc catacagttt cccgccgttg tacctcgccg
     1801 gcggcgaaac gacgatccga accggcggtt cgctgatgcc gtacacggac ctcaacgtgc
     1861 gcgatgcgat ccattccggc aaacgcatcg gacccgacat cgacgtcacc ggcccctacc
     1921 tcaatggcgt cgaatccttc accgaaaaca tgcacaccct gaccggcccg gccgatgcca
     1981 cgggcaccgt tgactactgg gccgccgagg gcgtcgactc gttcaaggcc tacgaccaca
     2041 tcacccgcgc ggaactcaaa gccgccatcg acgccgccca cgcgcaccac gacaagatca
     2101 ccgggcacct gtgctcggtc acgttccgcg aagcggcgga cctcggcatc gacaacctcg
     2161 aacacgcgtt cttcgtcgct tccgatttcg tgaaggacaa gaagccggac gagtgtccgc
     2221 aggatgtccg cgcctccatc gcgtcactgg acgtggattc gcccgaggtg acgtcattga
     2281 tcaggctgct ggtcgaaaag cacgtggcgc ttacttcgac gctcggggta ttcgaaacct
     2341 tcgtgcccgg ccggcccgcc ttgccgcggg tggccttgga catgctgaac cccgcactgc
     2401 gcgagctcta cgaagccacc tggaagaaag tgcaggcgat gcccgctccc cgcgcaaaga
     2461 tgatgcagca agagttcgcc aaggccaccc ggctcgaaaa gaagttcgtc gaagccggcg
     2521 gcgtgttgat gggcggcagc gatccgaccg gctacggcgg ggtgattccg ggcttttcct
     2581 ccaagcggca gatggaactg ctggtgcagg cggacggctt cgccttcccg caagcggtga
     2641 agatcatgtc gctcaatgcg gcgaagttcc agggacgcga cgaggacatc ggttccctcg
     2701 cagtcggcaa gcgcgccgac atcgtggtga tcgacggcga cccggcgaag gacgtcacag
     2761 ccatcgaaca catgccgctg gtgttcaagg acggcgtggg ttacgacacc gccgcgatct
     2821 tcaagtccat gcagggccag atcggcatga attgatcgtc cggcgggtgg atgcactcac
     2881 cacgcacggt cgtattgctc cggcacgcgc accgtttcgc ccagctcctt tgccgcacga
     2941 cgagggaagt acggatcgcg caggaattcg cgggccatct cgatcacgtc ggcatcgcca
     3001 cgctgaagga tttcctcggc ctgccgtggc tcggtgatca gcccgacggt tgacgtcggg
     3061 atgccggctt cacggcggat ccgcgcagcg aatggcacct ggtaaccggg gcccgtcgga
     3121 acgttcggat gcggcaccgt gccgcccgag gaacagtcga tcacgtccac gccatcggca
     3181 cggagcatgc gcgcaagctg cacgcattcc tcgatatccc agccgccctt cgcccagtcg
     3241 gtggccgaca gtcggaccca caacggcatc ggcgagggcc aggtttccct caccgcggca
     3301 atcacttccc gcagcagccg cgcgcgattt tccagcgaac cgccatattg gtcggtgcgc
     3361 gtgttcgaaa gcggcgagag gaactgatgg atcaggtagc cgtgggcgcc atggatttcg
     3421 atcacttcga aaccggcctc gcgtgcacgg cgcgcggcgg cgcggaagtc gtcgatggcc
     3481 ttcgcgatgc ccgatgcttc cagcgcggcc ggcacgggat agccttcatc gaacgggatc
     3541 gcgctcggcg cgaccggcgt ccagccgccc tgccctgcgt ccagcggacc gccgccgcgc
     3601 cacggcgcat cggtgctggc cttgcgcccc gcgtgcgcga gctgcatgcc cgcagcggcg
     3661 ccctgcccgc ggatgaacga tgcgatccgc gaccaggctt ccgcctgcg
//
LOCUS       DASSNH010000100         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_140743, whole genome shotgun sequence.
ACCESSION   DASSNH010000100 DASSNH010000000
VERSION     DASSNH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140743"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..523
                     /locus_tag="VFK00_03570"
     CDS             50..523
                     /locus_tag="VFK00_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7592065.1"
                     /translation="MNRTTTPPDTAFQLCRLIGRLRAEFVGAVEQEMARQHVDLTFMQ
                     FVALKLLGHEKPMTPVELARALHYNPGALTRLLDKLEEHGYLRRVPDAVDRRALRLEL
                     TTLGKALRKRVIGYCDAVAERIFAPVSPNERERLHGTLTKVLDHVQSGRESSHRD"
     gene            570..2060
                     /locus_tag="VFK00_03575"
     CDS             570..2060
                     /locus_tag="VFK00_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7592066.1"
                     /translation="MTLSGFRHRSGLAVFLIAGVLGGCASTGGLHPEAAPKAPQDLHA
                     EQSLAGVKVDAAAWPGKDWWKAIGDPQLDKLVDEAIAGNPGMTLADARVRAALATANA
                     ADASRKPTLNGGASVAGARVPPIMPPIANGHFGVIRYGYLSFSWNFDLWGGKRAAWEA
                     AVGNARAAEVDSRAARLKLSADVVQAYFNLAGAYQQRDLAKDELQRAQDFLGLTKKRV
                     ASGVDSRFQLARIQGETASARAQLEAADNAVHIDGLVLAALLGAGPDRALSIQRPKLP
                     AIPELALPSDLPAELLGRRPDVVAARWRVEASSQDIKAAKAKFLPNIGISSLVGLIAP
                     SSMNLLSLRNRFYTVSPALSLPIFEGGALRANLAGKNAAHDIAVAQYNQTLVHAINQV
                     AGQVDDLRSLGVQVADAETARSSAADAYKLAMQRYRAGVGNFLEALSVRQELITAEQQ
                     LAKLQTRRSDAWAKLNEALGGGFQPADDSPSLAATPDTRSEHKAHR"
     gene            2057..>3285
                     /locus_tag="VFK00_03580"
     CDS             2057..>3285
                     /locus_tag="VFK00_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrA/EmrK family multidrug efflux transporter
                     periplasmic adaptor subunit"
                     /protein_id="HET7592067.1"
                     /translation="MTSPINPNPVTEEDVAARQKAKRQRKRRWLLRGLVAVIVVVAII
                     WAIYHFMVGRWYESTDDAYVDGNVVQITPQVPGTVISIGAEDNDYVRRGQTLVKLDSS
                     SADVALDEAEAALAQAVRKVRGLYSTVHSSKADVAASEAAVEQARADYRRRKGLAASG
                     AISAEELAHAHDALTTAESALAAAQAQLATTSALVSDTEIASHPDVKAAAAKVRAAWL
                     DDDRTTIVAPVSGYVAKRTVQVGQRVQPGTPLMAVVPLHQVWVDANFKETQLREMRIG
                     QPVTLTSDVYGGDVTYHGHIAGLGIGTGSAFSLLPAQNASGNWIKIVQRLPVRIDLDQ
                     KELDKHPLRIGLSMNVEVNLHDQSGPMLSQQPPQHPVFSTDVYANDTTKVDALIARII
                     HANSGKAAAQVAGHPAVR"
BASE COUNT          585 a         1121 c         1102 g          477 t
ORIGIN      
        1 gagttataat tgccttgaca attaaagccg aaacagtatc atccacggca tgaatcgcac
       61 cacgactcca cccgacaccg ccttccagtt gtgccggctg atcggccggc tacgggcgga
      121 attcgtcggc gcggtcgagc aggaaatggc gcgccagcac gtcgatctca ccttcatgca
      181 attcgtggca ctcaagctgc tcggccacga gaagccgatg acgccggtcg aactggcgcg
      241 ggcgctgcac tacaatccgg gggcgcttac ccgcctgctc gacaagctcg aggagcatgg
      301 ttacctgcgg cgggttcctg acgccgtcga tcggcgtgcc ttgcggctcg aactgaccac
      361 gctgggcaag gcgctccgca agcgcgtgat cggctactgc gacgcggtgg ccgagcgtat
      421 ctttgccccg gtctcgccga acgagcgcga gcggctgcac ggcaccctca cgaaggtgct
      481 ggaccacgtt cagtccgggc gcgaatcttc ccaccgggat tgaatcgccg gccccggccc
      541 caacctctgc cactcttccg gctcaagcca tgacactttc gggtttccga caccgtagcg
      601 gacttgctgt tttcctgatc gccggcgtgc tcggcggctg cgccagcacc ggtggcctgc
      661 atccggaggc ggccccgaag gcgccgcagg acctgcacgc ggagcagagc ctcgccggtg
      721 tgaaagtcga cgccgcggcg tggccgggca aggattggtg gaaggcgatc ggcgacccgc
      781 agctcgacaa gctggtcgac gaggcgatcg cggggaaccc cgggatgacc ttggccgatg
      841 cgagggtgcg tgccgcactg gccactgcca acgcggccga cgcctcccgc aagccgaccc
      901 tgaacggcgg cgccagcgtg gccggggccc gggtgccgcc gatcatgccg ccgatcgcga
      961 acggccattt cggcgtcatc cgctacggct acctcagctt cagctggaac ttcgacctct
     1021 ggggcggcaa gcgcgccgcg tgggaagccg ccgtcggcaa tgcgcgcgcg gccgaagtcg
     1081 acagccgggc ggcgcggctg aagctgtccg ccgacgtggt gcaagcctat ttcaacctgg
     1141 ccggggcata ccagcagcgc gacctcgcga aggacgagtt gcagcgcgca caggatttcc
     1201 ttggcctcac gaagaaacgt gtcgccagcg gggtcgacag ccgcttccag ctcgcccgga
     1261 tccagggcga aacggcgagc gcgcgggcgc aactagaagc ggccgacaat gcggtgcaca
     1321 tcgatggcct ggtgttggcc gcattgctcg gagcgggccc cgatcgcgcg ctctcgatcc
     1381 agcgcccgaa gctgccggcg atcccggagc tcgcgctgcc gagcgacctg ccggccgaac
     1441 tgctgggccg gcgtcccgac gtggtcgccg cacgttggcg tgtcgaggca tccagccagg
     1501 acatcaaggc cgccaaagcc aagttcctgc cgaacatcgg aatcagctcg ctggtggggc
     1561 tgatcgcgcc gtcctcgatg aacctgctct cgctgcgcaa ccgcttctac acggtctcgc
     1621 ccgcactcag cctgccgatt ttcgagggcg gagcgctgcg cgcaaacctt gccggcaaga
     1681 acgcggcgca cgacatcgcg gtcgcgcagt acaaccagac attggtacac gcgatcaacc
     1741 aggtggcggg gcaggtcgac gacctgcgtt cgctcggggt gcaggtcgcg gacgcggaga
     1801 cggcgcgcag cagcgccgcc gatgcctaca agctcgcgat gcagcgctat cgcgccggcg
     1861 tgggcaactt ccttgaagcg ctgagcgtgc gccaggagtt gatcaccgcc gagcaacaac
     1921 tggcgaagct gcagacgcgc cgaagcgatg cgtgggcgaa gctcaacgaa gccctgggcg
     1981 gcggcttcca gccggcggac gatagtccgt cactggccgc gacgcccgac acccgatccg
     2041 aacacaaggc acatcgatga cctccccgat caatccgaac ccggttaccg aagaagacgt
     2101 cgccgcccgg cagaaggcga agcggcagcg caagcgccgc tggttgttgc gcggcctggt
     2161 ggccgtgatc gtcgtcgtcg cgatcatctg ggccatctac cacttcatgg tcgggcgctg
     2221 gtacgaaagc accgacgatg cgtacgtcga tggcaacgtg gtgcagatca cgccgcaggt
     2281 gccgggcacc gtgatcagca tcggtgccga ggacaacgac tacgtgcggc ggggacagac
     2341 cctggtgaag ctcgactcgt ccagcgcgga cgtggcgctc gatgaagccg aagccgcgct
     2401 cgcccaggcc gtgcgcaagg tccgcggcct gtacagcacg gtgcacagct ccaaggcgga
     2461 cgtcgcggcc agcgaagcgg cggtcgagca ggcccgcgcc gactaccggc gccgcaaggg
     2521 cctggccgcg tcgggagcga tctccgccga ggagctcgcc catgcccacg acgcgctgac
     2581 caccgcggaa agtgcgttgg ccgccgccca ggcgcagctc gcgaccacca gcgcgctggt
     2641 atcggacacc gagattgcct cgcatccgga cgtcaaggcc gccgcagcca aggtgcgtgc
     2701 agcctggctc gacgacgacc gcacgacgat cgtcgcacct gtttccggct acgtcgccaa
     2761 gcgcaccgtg caggtcggcc agcgcgtgca gccgggaacg ccgctgatgg ccgtggtgcc
     2821 gttgcatcag gtgtgggtcg atgccaactt caaggaaacc cagttgcgcg agatgcgcat
     2881 cgggcaaccg gtgacgttga cctcggacgt gtacggcggc gacgtaacct accacggcca
     2941 catcgcggga ctcggcatcg gcaccggcag cgcgttctcg ctgttgccgg cgcagaacgc
     3001 gtccggcaac tggatcaaga tcgtgcagcg cctgccggtg cgcatcgacc tcgaccagaa
     3061 ggaactcgac aagcatccgt tgcggatcgg gttgtcgatg aacgtggagg tgaacctcca
     3121 cgatcagtcc ggtccgatgc tgtcgcagca gccgccgcag cacccggtat tcagcaccga
     3181 cgtgtacgcg aacgacacga ccaaggtcga tgccttgatc gcccggatca tccatgccaa
     3241 cagcggcaag gctgcggcgc aggtggcggg gcaccccgcc gtgcg
//
LOCUS       DASSNH010000101        11149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_640076, whole genome shotgun sequence.
ACCESSION   DASSNH010000101 DASSNH010000000
VERSION     DASSNH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640076"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFK00_03585"
     CDS             <1..843
                     /locus_tag="VFK00_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592068.1"
                     /translation="KLQRSFRHTATAEQLAFIGEDNISNEGAVRNLMGMAPNDYVYAG
                     KTTKLARFLPGVKVTVLGPPTVKQHAGVRKQRATDPDEFWSLQGKALGVGGKAGSIDM
                     TLFPGFGQTTGGGAPLWARWVIKSLRSAHGDQLLELVRSLDTAMNNTSVVLLFECGGH
                     RFLFPGDAQIENWEYALGQKKFTELLRNVTVYKVGHHGSLNATPKTLWEEWYPKGAKP
                     LALDQRMTSFMSTKSGKHGSVASGTEVPRTKLVSALKKWTRLHSTQDLEEALYVDESF
                     EVRG"
     gene            855..2192
                     /locus_tag="VFK00_03590"
     CDS             855..2192
                     /locus_tag="VFK00_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592069.1"
                     /translation="MARTDIRCVRYAFHPVGQGLFASGALGNCPSRWMGREKGDVYCL
                     WVYDCGTASAKALLENALDRFAAATAAKKALLDLVVLSHFDKDHISGVVDLLGRFEVD
                     VLLLPFVPLAQRIVLAFHEGIAIDDPLFAFFINPVGFLNGAGVRGVRRVVFVPPANGE
                     GPGPVEAGNNSPDRPPPDRWPEHEDAYRLDFDSDPFPDGVFGVADEPKGKIGPATMLR
                     PGSALRAASVWEFVPYNDETLALQPGESWLKKVGTLRDELLGEKQREQRAKLLSHLKD
                     EYDEEFGTGSKGRNQISLFLYAGPVSASAPDGCRGNYGWAFKADPPFIASGELISGWR
                     AADRPGKRAAVLYTGDGYLDSSTRVDALLRFMGNARIDRTAVLQVMHHGAEANWTPGV
                     AAAIGPWFSVFSSDPNHRGFGHPHAEVLRDFWLCGPVQVKDDSDAEIHVWWEW"
     gene            complement(2232..2855)
                     /locus_tag="VFK00_03595"
     CDS             complement(2232..2855)
                     /locus_tag="VFK00_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7592070.1"
                     /translation="MTREGTGAVLLAAGCANRYGAPKQLIPVAGMPMVRHVARIAMQC
                     IERTVVVTGAYRVEVEACVSDLDLQLAFTSDWQRGMGGSIACGVERLLRLDPEVHACL
                     IMLADQPLITAADLAALVDARREHPEHIIASGYAGETCGAPCLFPRRCFEELLALQGP
                     AGARSILRAHRNEVHPVANPRADIDIDTPEDRRQLPSDVATGYTPIN"
     gene            complement(2852..3937)
                     /locus_tag="VFK00_03600"
     CDS             complement(2852..3937)
                     /locus_tag="VFK00_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7592071.1"
                     /translation="MNSPQEFRRLFAAANRLRASDAAGALATIVRTAGSTYRRPGTSM
                     LVGADGSVTCPLAGGCPQHDLVLRARRTIEADVPEIARYDRDAGFDVLLEAGCGGELE
                     VLIEPLSRDQDFAFLDAIATLHERRARGFMATIFCRNGTVLSPRPQRLLAGDGVAWQD
                     IGDPSLCERVAEIGGDLRGERAAARRLAVGRASMEVLFESLRPVHRLVIFGANEGAVA
                     LARVAASLGWSCVVADPSPDRAADAMDGVDVVAATPETIRRSIAFDRDTSVAAMTHNL
                     ERDVRLLQALAGVPLAYLGVIGSRERVALIRARLPVAMDLHAPAGLDIGSETPEEIAL
                     AVAAEIIACSHARTAGSLAASALPIHP"
     gene            complement(3945..6059)
                     /locus_tag="VFK00_03605"
     CDS             complement(3945..6059)
                     /locus_tag="VFK00_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7592072.1"
                     /translation="MNADAANAPLEIRTIGTPLERIEGHAKVAGTAPYAYEHALADPL
                     YVCPLQSTIARGRVASMAIDAAKPLHGVVAIITHENAPRLAWPEDESNRNRELWILQS
                     PEIGFRGQFIGAVIAESLEVALQAANLVRVRYDEEPHDAELRADRPDLYAPEKVNAGF
                     ATDTEEGDPESAFESAAVQIDHTYTTPMEHNNPMETHTTVARWDGGRFTLHDSTQGAH
                     SLRKTLAPLFDLQPEDFHVIAPHVGGGFGSKGVAHAHDALACLAARAVPGRAVKVTLT
                     RQQMFALAGYRTPTIQRFRLGADAQGRLTAIIHDVVEQTSRIKEFAEQTAAPTRIMYA
                     APNRRTTHRLAALDVPVPSWMRAPGETPGMYAGEVAMDELALACGIDPIELRIRNEPE
                     VDPESGKPWSGRDLVGCLREGARRFGWQDRDAEPRTRREDGWWIGMGVASSTYPRHSM
                     PGSVATIRRGSDGRYAVRIGAVDIGTGTWTALTQIAADALACPVEAIKLEIGDTNHPN
                     ATVEGGSSGISSWGPAVVAAARKLRQEHGEDPAVGAEATAGMPENPDEERFAMHSFGA
                     QFAEVHVHADTGEIRVARMLGVFSAGRIINPRLARSQFIGGMTMGLSMALLENSVLDT
                     RFGHVVTHDFAEYHIAANADVPEIEAIWLDESDPHSNPMGSRGIGEIGIVGAAAAVAN
                     AAWHATGIRVRDLPITADKLLG"
     gene            complement(6056..7048)
                     /locus_tag="VFK00_03610"
     CDS             complement(6056..7048)
                     /locus_tag="VFK00_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7592073.1"
                     /translation="MNPFAYQRADDAASAVAAVAGDPEAMFLGGGTNLVDHMRLGIAK
                     PDRLVDVTRLPFDRIAAIPGGGVRIGANVRNSDLAADRMIRARYPVLSQALLSGASGQ
                     LRNLATTGGNLLQRTRCVYFQDVTTPCNKREPGTGCSAIGGYTRYHAILGASDQCIAV
                     HPSDMAVAMAALDATVIVLGRGGERRIPVIDFHRLPGDAPDKDTVLQHGELITAVELP
                     ALPFATHSTYRKVRDRASFAFALVSVAAALDVDAGKVRNVRIALGGVAHKPWRASRAE
                     AALGGRPATIEQFERAADAELAAAQPQEGNAFKVKMARNTLVAVLRDLAGAASE"
     gene            complement(7045..7557)
                     /locus_tag="VFK00_03615"
     CDS             complement(7045..7557)
                     /locus_tag="VFK00_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7592074.1"
                     /translation="MEERITLRVDGVERSLTVDTRVTLLDALRNLLGNMSPKKGCDHG
                     QCGACTVLLDGRRATTCLALAVAHDGATIVTAAGLAADGLHPMQRAFVQHDALQCGYC
                     TPGQVCSAVGMLEEAAEGQPSYVTADLDGDVELTAAEIRERMSGNLCRCGAYVNIVAA
                     IREVAREQAP"
     gene            complement(7731..8477)
                     /locus_tag="VFK00_03620"
     CDS             complement(7731..8477)
                     /locus_tag="VFK00_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7592075.1"
                     /translation="MIEHFQLRLADSHMLAPTVRHLAFERADGTPFKFTPGQFIQIHF
                     HYEDGKPTKRSYSVGTPADDSGHVRQLQLAVSYVEGGAATKLFDGLEAGGEIEASGPY
                     GRFCLMDDDANRRYLLVATGTGITPYRAMLPRIEKLITEQGRRFVLLYGARNEQELLY
                     GEEFEAFAQKHPEFTFHPCLSRAARAAPRSNDRRGHVQDMLSELGVDPEHDIAYLCGN
                     PDMIDATFTELKELGMPIKHIRREKYVSSR"
     gene            8621..9598
                     /locus_tag="VFK00_03625"
     CDS             8621..9598
                     /locus_tag="VFK00_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7592076.1"
                     /translation="MPATTPSDAIPALEVEALRKTYGNGVQALKGVSLTVRQGDFFAL
                     LGPNGAGKSTLIGILSSLVNPTAGDARVFGISIHDDRGGVMRQIGLVPQEINFNQFET
                     PFDICVNVAGFYGIPRSVAKRRAEKYLNELRLWDKAFQATRHLSGGMKRRLMIARAMM
                     NEPKLLILDEPTAGVDIEIRRSMWQFITGINRTGTTVILTTHYLEEAEQLCRNIAIID
                     HGEIIEDTSMRRLLRKLDVETFVLDVTGIEGALPEVEGVRLTRVDELTLEAELPSGRD
                     LNSLFAALTAHGITVTSMRTKTNRLEELFVRLVERGNGDARNAKGRAQA"
     gene            9646..10425
                     /locus_tag="VFK00_03630"
     CDS             9646..10425
                     /locus_tag="VFK00_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7592077.1"
                     /translation="MGGVGRANMVALYTIARREAHRVLRIWTQTLVPPAITMTLYFVI
                     FGKVIGSRVGTMDGFPYMQYIVPGLVMMSIINNCYSNITSSFFGSKFGRYVEELLASP
                     MPSWVVLTGYVAGAVMRGVLVGIIVMAIALFFTSLHIAHPLVAVTTVILGATIFSLAG
                     FINAIFAKRFDDIAIVPTFILQPLSYLGGVFYSVKLLGSPWQEVSLVNPILYFVNAFR
                     FGILGVSDVPVWGAYVVMLAFVIVLGAVALWLMNKGVGLRS"
     gene            10538..>11149
                     /locus_tag="VFK00_03635"
     CDS             10538..>11149
                     /locus_tag="VFK00_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592078.1"
                     /translation="MKGVIAAAVALVCITWCTHVRAQVFKCTAANGAVSYQDHACGHH
                     ESQTVMKVPGQSPPNYVPPAPASSPQPASTPPLPTPIYVPPPSPLPAMYACVGAVNGE
                     PYLTRSPPPPYLAPLGVMGYPPRTLSQAYGAPGGAGMSAPELSKPPIGGPPVATGMTE
                     VQDYCLPATRAQVCGFVQRDYDENHRKLRMAMPREQPPYEQRER"
BASE COUNT         1785 a         3677 c         3801 g         1886 t
ORIGIN      
        1 aagctgcaac gctcgttccg gcacaccgcg accgccgagc agttggcgtt catcggcgaa
       61 gacaacattt ccaacgaggg cgccgtccgc aacctgatgg ggatggcgcc caacgattac
      121 gtgtacgccg ggaagaccac caaactggca cggttcctgc ccggcgtgaa ggtgacggtg
      181 ctgggtccgc cgacggtgaa acagcatgcc ggcgtacgca agcagcgcgc caccgatccc
      241 gacgaattct ggagcctgca aggcaaagcc ttgggcgtcg gcggcaaggc tggctcgatc
      301 gacatgacct tgttcccggg cttcggacaa acgaccggtg gaggcgcgcc gttgtgggcg
      361 cgctgggtca tcaagtcctt gcgcagcgcg catggcgacc agctgctgga actggtgcgc
      421 tcgctcgaca ccgccatgaa caacaccagc gtggtcctgc ttttcgagtg cggcgggcac
      481 cggttcctgt ttcccggcga tgcgcagatc gagaactggg agtacgcact cgggcagaag
      541 aagttcaccg agctgctcag gaacgtcacg gtctacaagg tcgggcacca cggaagcctc
      601 aacgcgacgc cgaagacgct ctgggaggag tggtacccga agggcgccaa gccgctcgcc
      661 ctggatcagc gcatgacgtc cttcatgtcc accaagtccg gcaagcacgg gagtgtcgcc
      721 agcggcaccg aggtcccgcg caccaagctc gtatccgcac tcaagaagtg gacgcggctg
      781 cattccacgc aggacctcga agaagcgctg tacgtggatg aaagcttcga ggtccggggt
      841 tgaacggagg gcgcatggcc cgtactgaca tccgctgcgt tcgatacgca ttccatccgg
      901 tggggcaggg gcttttcgca tccggcgccc tcgggaattg cccctccagg tggatgggcc
      961 gtgaaaaagg cgatgtgtat tgcctgtggg tctatgactg cggcaccgcg tccgccaagg
     1021 cactgctcga gaacgcactc gatcggttcg cggcagcgac ggctgctaag aaggcactcc
     1081 tggacctggt ggtactctcc catttcgaca aggatcacat ctcgggcgtg gtcgacttgc
     1141 tgggacgctt cgaggtcgac gtcctcctcc ttcccttcgt gccgcttgcg cagcggatcg
     1201 ttcttgcatt ccacgagggc atcgccatcg atgacccgct gttcgcgttc ttcatcaatc
     1261 ccgtgggatt cctcaacgga gccggtgttc ggggcgtccg ccgcgtcgtg ttcgttccac
     1321 ccgcgaatgg cgagggtcca gggccggtgg aagccggcaa caacagtccg gatcgacccc
     1381 ctccggatcg gtggccggaa cacgaggacg cttatcggtt ggacttcgat tccgatccct
     1441 tcccggacgg tgtcttcggt gtcgccgacg agccaaaggg caagatcgga cccgccacga
     1501 tgcttaggcc gggcagcgcg ctgcgtgcag cgtcggtttg ggaattcgtg ccgtacaacg
     1561 acgagacact ggcgctgcaa cccggggaga gctggctaaa aaaggtaggg acgttgcgcg
     1621 acgagcttct cggcgaaaaa caaagggagc agcgcgcgaa gctcctttca cacctcaagg
     1681 acgagtacga cgaggagttc ggcaccggtt caaaagggcg aaaccagata tcgctgttcc
     1741 tctatgcagg ccccgtcagt gcctcggctc cggacgggtg tcgtggcaac tatgggtggg
     1801 cattcaaggc cgatccgccg ttcattgctt ccggtgaact gataagcgga tggcgcgccg
     1861 cggatcgtcc gggaaaacgc gcggccgtgc tttacacggg cgacggctac ctggactcga
     1921 gcactcgcgt ggacgcgttg ctgcggttca tgggtaatgc acgcatcgac cgtacggccg
     1981 tgttgcaggt gatgcaccac ggggccgagg ccaactggac ccccggcgtc gccgcggcca
     2041 tcggtccgtg gttcagcgtg ttcagcagcg acccgaacca tcggggattt gggcatccgc
     2101 atgccgaggt gttgcgcgac ttctggcttt gcggtccggt gcaggtcaag gatgattccg
     2161 acgccgaaat ccatgtgtgg tgggagtggt gatggaagcc ctgcgcgtcg ctgcgcgcat
     2221 ggtgtgcgct gtcaattgat tggggtgtag ccggtcgcga cgtcgctggg caactgtcgg
     2281 cgatcctccg gcgtgtcgat gtcgatgtcg gcgcgcggat tggctacggg gtggacctcg
     2341 ttgcgatggg cgcgcaggat ggagcgcgcg ccggcagggc cttgcaaggc caggagttcc
     2401 tcgaagcagc ggcggggaaa caggcaaggt gcgccacatg tctcgccggc gtagccggag
     2461 gcgatgatgt gctccggatg ctcccggcgc gcatcgacca gtgccgcgag atcggcggcg
     2521 gtgatgaggg gctggtcggc gagcatgatc aggcaggcgt gaacctcggg gtccagccga
     2581 agtagacgtt cgacaccgca ggcgatcgag ccgcccattc cgcgttgcca gtcgctggtg
     2641 aaggcaagct ggagatcgag atcgctgacg cacgcttcga cctcgacgcg gtaagcgccg
     2701 gtcacgacca ccgttcgctc gatgcattgc atcgcaatcc gggcgacgtg ccgcaccatc
     2761 ggcattcccg cgaccgggat gagctgtttg ggcgcgccgt aacggttggc gcagccggcc
     2821 gccagcagca ccgcgccggt cccttcgcgt gtcatgggtg gatgggcagc gcggacgccg
     2881 caaggctgcc ggcggtgcgc gcgtgcgagc acgcgatgat ttccgcggcc acggcgagcg
     2941 caatttcctc cggcgtctcg gaaccgatgt cgaggcctgc cggcgcgtgc aggtccatcg
     3001 ccacgggcag ccgcgcccgg atcagcgcca cgcgctcgcg cgagccgatc acgccgaggt
     3061 atgccagcgg aacgccggcc aacgcctgca gcagccgcac gtcgcgttcg aggttgtggg
     3121 tcatcgccgc gaccgaggtg tcgcgatcaa acgcgatcga tcggcgtatc gtctccggcg
     3181 tggccgccac gacatccacg ccgtccatgg cgtcggctgc gcggtccggc gacggatcgg
     3241 ccaccacgca cgaccacccg aggctcgcgg ccacccgcgc caacgcgacg gcgccttcgt
     3301 tggcgccgaa gatcacgagc ctgtgcaccg ggcgcaagga ttcgaacaac acttccattg
     3361 aagcccggcc gacggcgagt cgccgggcgg ctgcgcgttc gccgcgcaga tcgccgccga
     3421 tttcggcgac gcgttcgcac agcgatggat caccgatgtc ctgccatgca acgccgtcgc
     3481 ctgcgagcag ccgttgcggg cggggcgaca gcaccgtgcc gttgcggcag aaaatcgtcg
     3541 ccatgaaacc gcgggcgcgg cgctcgtgca gcgtggcgat cgcatccagg aaggcgaagt
     3601 cctggtcgcg cgatagcggt tcgatcaaca cctccaattc gccaccgcaa ccggcttcga
     3661 gcagcacgtc gaaacccgca tcgcggtcgt agcgggcgat ctcgggcacg tcggcttcga
     3721 tcgttcgccg cgcgcgcagc accaggtcgt gctgcgggca tccgcctgcg agcggacaag
     3781 tgacgctgcc gtccgcgccg accagcatgc tcgtgcccgg ccgccggtag gtggagccgg
     3841 cggtgcggac gatcgtcgcg agcgccccgg cggcatcgct cgccctcaat cggttcgcgg
     3901 ccgcgaacag ccgccggaat tcctgcggcg aattcatggg ttcatcagcc gagcagcttg
     3961 tccgcggtga tgggcaggtc gcgcacgcgg atgccggtgg cgtgccaggc ggcgttggcg
     4021 accgccgcgg cggcgccgac gatgccgatc tccccgatcc cgcgcgaacc catggggttg
     4081 gagtgcggat cgctttcgtc cagccagatc gcctcgatct ccggcacgtc cgcgttggcg
     4141 gcgatgtggt actcggcgaa gtcgtgcgtc accacgtgcc cgaagcgggt gtccagcacg
     4201 ctgttttcga gtagcgccat cgacagcccc atcgtcatgc cgccgatgaa ctgcgagcgt
     4261 gcgaggcgtg gattgatgat ccgcccggcc gagaacacgc cgagcatccg ggcgacgcgg
     4321 atctcaccgg tgtcggcgtg cacgtgcact tccgcgaact gcgccccgaa cgaatgcatg
     4381 gcgaagcgct cctcgtcggg gttctccggc atgcccgcgg tcgcctccgc gccaacggcg
     4441 ggatcctcgc cgtgctcttg gcgaagcttg cgcgccgcgg cgaccaccgc cggtccccag
     4501 gaactgatgc cgctcgagcc gccttccacg gtggcgttgg gatggttggt gtcgccgatc
     4561 tccagcttga tcgcctcgac cgggcaggcc agcgcgtcgg cggcgatctg ggtcagcgcg
     4621 gtccaggtgc cggtaccgat gtccaccgca ccgatccgta ccgcgtagcg cccgtccgat
     4681 ccgcggcgga tggtcgcgac cgagcccggc atcgaatggc gcggataggt cgaactcgcg
     4741 acgcccatgc cgatccacca gccgtcttcc cggcgcgtgc gcggctcggc atcgcgatcc
     4801 tgccagccga accggcgggc accttcgcgg aggcaaccga ccaggtcgcg acccgaccat
     4861 ggcttgcccg attcgggatc gacctccggt tcgttgcgga tgcgcagttc gatcggatcg
     4921 atgccgcagg cgagcgccag ttcgtccatg gccacttcgc ccgcgtacat cccgggcgtt
     4981 tcgccgggcg cgcgcatcca cgaggggacc ggcacgtcga gcgcggcgag ccgatgggtg
     5041 gtgcggcggt tcggcgcggc gtacatgatc cgggtcggcg ccgcggtctg ttccgcgaat
     5101 tccttgatgc gcgaggtctg ctcgaccacg tcgtggatga tcgcggtgag gcgcccctgc
     5161 gcgtccgcgc cgagccggaa ccgctggatc gtgggcgtgc ggtagccggc cagcgcgaac
     5221 atctgttggc gcgtcagcgt caccttcacg gcgcggccgg gcaccgcgcg ggcggcgagg
     5281 caggccagtg catcgtgcgc gtgtgcgacg cccttggatc cgaaaccgcc gccgacgtgt
     5341 ggcgcgatga cgtggaagtc ctcgggctgc aggtcgaaca acggcgccag cgtcttgcgc
     5401 aacgaatgcg cgccctgcgt cgagtcgtgg agggtgaacc ggccgccgtc ccaacgcgcg
     5461 acggtggtgt gcgtctccat cggattgttg tgttccatcg gggtggtgta ggtatggtcg
     5521 atctgcaccg ccgcggattc gaacgccgat tccggatcgc cctcctcggt atcggtggcg
     5581 aagccggcgt tcaccttttc cggcgcatat aaatccggac ggtccgcgcg aagctcggca
     5641 tcgtgcggct cctcgtcgta gcgcacccgc accaggttcg cggcctgcaa cgcgacttcc
     5701 agactttcgg cgatgaccgc gccgatgaac tggccgcgga agccgatctc cggcgactgc
     5761 aggatccaca gttcccgatt ccgattcgac tcgtcctcgg gccaggcgag ccgcggtgca
     5821 ttctcgtgcg tgatgatcgc gaccacgccg tgcagcggct tggccgcgtc gatggccatg
     5881 gatgcgaccc ggccgcgggc gatggtcgac tgcagagggc acacgtacaa cggatcggcg
     5941 agcgcgtgtt cgtacgcgta cggcgcggtg ccggcgacct tggcgtgtcc ctcgatgcgc
     6001 tccagcggtg tgccgatggt gcgtatttcc agcggggcgt tggctgcgtc ggcgttcatt
     6061 ccgatgccgc cccggcgagg tcccgcagta cggccaccag cgtattgcgg gccatcttca
     6121 ccttgaaggc attgccttcc tgcggctgcg ccgcggcgag ctccgcgtcc gccgcgcgct
     6181 cgaactgctc gatcgttgcc ggccggcctc ccagcgccgc ttcggcccgg ctcgcccgcc
     6241 acggcttgtg agcaacgccg cccagcgcga tccgcacatt gcgtaccttg ccggcatcga
     6301 cgtcgagcgc cgccgcgacc gacaccagcg cgaaggcgaa ggaagcgcga tcgcgcacct
     6361 tgcggtacgt ggaatgcgtc gcgaacggca gggccggcag ttcgacggcg gtgatcaatt
     6421 cgccgtgctg gagcaccgtg tccttgtcgg gcgcgtcgcc cggcaggcga tggaaatcga
     6481 tgaccgggat ccgtcgctcg ccgccgcgcc ccagcacgat gaccgtggca tccagcgccg
     6541 ccatcgcgac cgccatgtcc gacgggtgca cggcgatgca ctggtcggag gcgccgagga
     6601 tcgcgtggta gcgagtgtag ccgccgatcg ccgaacatcc ggtgccgggc tcgcgcttgt
     6661 tgcagggcgt ggtcacgtcc tggaagtaca cgcaacgcgt gcgttgcagc aggttgccgc
     6721 cggtggtggc gaggttgcgg agttgccccg aggcccccga aagcagcgcc tgcgacaaca
     6781 ccggatagcg ggcgcggatc atccggtcgg cggcgaggtc gctgttgcgg acgttcgcgc
     6841 cgatgcgcac gccgccgccc ggaatcgccg cgatgcgatc gaagggcaga cgcgtcacat
     6901 cgaccaggcg atcgggcttc gcgatgccca ggcgcatgtg gtccaccagg ttggtgccgc
     6961 cgcccaggaa catcgcctcc ggatcgcccg caactgccgc gaccgcactg gccgcatcgt
     7021 ccgcgcgctg gtacgcgaac gggttcacgg cgcctgctcc cgcgcgacct cccggatcgc
     7081 ggcgacgatg ttgacgtagg cgccgcagcg gcacaggttg ccgctcatgc gctcgcggat
     7141 ctcggcggcg gtgagttcca cgtcgccatc caggtcggcc gtcacgtagc tcggttggcc
     7201 ttccgccgcc tcctcgagca tgccgaccgc cgagcagacc tgccccggcg tgcaatagcc
     7261 gcactgcaag gcatcgtgct ggacgaatgc acgctgcatc ggatgcaggc cgtccgccgc
     7321 gagacctgcc gcggtgacga tcgtcgcgcc gtcatgcgcc acggcgaggg cgaggcaggt
     7381 cgtcgccctg cgcccatcca gcaggaccgt gcaggcgccg cactggccat ggtcgcagcc
     7441 ctttttgggc gacatgttcc cgagcagatt gcgcagcgcg tccaggagcg tgacccgggt
     7501 gtcgacggtg agcgaacgct cgacaccgtc cacccgcagc gtgattcgct cttccatgaa
     7561 tctgctccgc ttgccggcgc gcctgcgccc tcacggaaca accgaccccc gaacgtccgg
     7621 cgcgcaaaag acaagcgtgg atttgcaccc gtgaacccca tgtgaagcca cgttacccag
     7681 cgtgtggcga gccgtccctc cgctcacctg catcccggcg gaagtcgaag ctaccgcgag
     7741 gaaacgtact tctcgcggcg gatgtgcttg atcggcatcc cgagctcctt caattcggtg
     7801 aaggtggcgt cgatcatgtc ggggttgccg cacaggtacg cgatgtcgtg ctcggggtcc
     7861 acgccgagtt cggacaacat gtcctggacg tggccgcggc gatcgttcga ccgcggcgcc
     7921 gcgcgcgcgg cgcgcgacag gcaggggtgg aacgtgaact ccgggtgctt ctgcgcgaat
     7981 gcctcgaact cctcgccgta caggagttcc tgctcgttgc gcgcgccata cagcagcacg
     8041 aagcggcggc cctgttcggt gatgagcttc tcgatgcgcg gcagcatcgc gcggtagggc
     8101 gtgatcccgg tgccggtggc gaccaggaga taacgccggt tggcatcgtc gtccatgagg
     8161 cagaagcggc cgtagggccc gctggcctcg atctcgccgc cggcctcgag cccgtcgaac
     8221 agcttggttg cggcgccgcc ctcgacgtac gagacggcca actgcagttg ccgcacgtgg
     8281 cccgagtcgt cggccggggt gccgaccgaa tagctgcgct tggtgggctt gccgtcttcg
     8341 taatggaaat ggatctggat gaactggccg ggcgtgaact tgaacggagt gccgtcggcg
     8401 cgctcgaacg ccaggtggcg gacggtgggc gcgagcatgt ggctgtcggc tagtcgtagc
     8461 tggaaatgtt cgatcatgtg cgcgggcgca acggcaagcg tgcgccgagt atggggttgc
     8521 gggtccgggc agccctgcca ttcggcatgg ggtcccgcgg gcgaaacttc tataataggg
     8581 gatggccgca gattccgttg ctccgaacgc cactcccccg atgcctgcca ccactccctc
     8641 cgacgccatc ccggcgctcg aagtggaagc cttgcgcaag acctacggca acggcgtgca
     8701 ggcgctgaag ggtgtgtcgc tgaccgtccg ccagggcgac ttcttcgcgt tgctggggcc
     8761 gaacggcgcc ggcaaatcca cgctgatcgg catcctttcc tcgttggtga acccgaccgc
     8821 gggcgatgca agggtattcg gcatctccat acacgacgac cgcggcggcg tcatgcgaca
     8881 gattgggttg gtgccgcagg aaatcaactt caaccagttc gagacgccgt tcgacatctg
     8941 cgtcaacgtg gccgggttct acggcatccc gcgctcggtc gccaagcgcc gcgccgagaa
     9001 atacctgaac gagctgcggc tgtgggacaa ggcgttccag gccacgcgcc acctttcggg
     9061 cggcatgaag cgccggctga tgatcgcgcg ggcgatgatg aacgagccga agctgctgat
     9121 cctcgacgaa ccgaccgcgg gcgtggacat cgagatccgg cgctcgatgt ggcagttcat
     9181 caccggcatc aaccgcaccg gcacgaccgt gatcctgacc acccattacc tcgaggaagc
     9241 cgagcagctc tgccgcaaca tcgcgatcat cgaccacggt gagatcatcg aggacacctc
     9301 gatgcgcagg ctgctgcgca agctcgacgt ggaaaccttc gtgctggacg tcaccggaat
     9361 cgaaggcgcg ctccccgaag tcgagggcgt gcgtttgacc cgcgtggatg agttgacgct
     9421 ggaggcggag ctgccgagcg gacgggattt gaattcgctg ttcgcggcgc tcaccgcgca
     9481 cggcatcacg gtgacctcga tgcgcaccaa gaccaatcgg ctggaagaac tgttcgtgcg
     9541 gctggtcgag cgcggcaacg gcgatgcgcg aaacgccaag ggcagggcgc aggcatgaac
     9601 gacccccgcg aaattgccgg atcatccatc gtggcgcggc cggcgatggg cggggtcggg
     9661 cgcgccaaca tggtcgcgct gtacacgatc gcgcgccgcg aagcgcaccg cgtattgcgg
     9721 atctggaccc agacgctggt gccgcccgcg atcaccatga ccttgtattt cgtgatcttc
     9781 ggcaaggtga tcggcagccg cgtcggcacg atggacggat tcccgtacat gcagtacatc
     9841 gtgccgggcc tggtgatgat gagcatcatc aacaactgct actccaacat cacctcgagc
     9901 ttcttcggtt ccaagttcgg gcgctatgtc gaggagctgc tggcgtcgcc gatgccgagc
     9961 tgggtggtgc tgaccggtta tgtcgccggc gccgtgatgc gcggggtgct cgtgggcatc
    10021 atcgtgatgg cgatcgcgct gtttttcacg tccctgcaca tcgcacatcc gctggtcgcg
    10081 gtcaccaccg tgatcctcgg cgccacgatc ttctcgctgg ccggtttcat caacgcgatt
    10141 ttcgccaagc ggttcgacga tatcgcgatc gtcccgacct tcatcctgca gccgctgagt
    10201 tacctgggcg gagtgttcta ttcggtcaag ctgctcggct cgccctggca ggaagtctcg
    10261 ctggtcaacc cgattctgta tttcgtgaat gcgtttcgct tcggtattct cggtgtttcg
    10321 gacgtgccgg tgtggggcgc ctacgtcgtg atgctggcct tcgtcatcgt gctcggcgcg
    10381 gtcgcgctgt ggctgatgaa caagggcgtg gggctgcgct cgtagcgctg cagcgtccgg
    10441 gtcgcgacca tggatcgctc ctacagcgcc ccgccgcaga ccgcggtctc cggtgccgtc
    10501 gcgcgatcag cgccgcagtt gcgcgtaaga tcgcagcatg aaaggtgtga tcgccgccgc
    10561 cgttgcattg gtctgcatca cctggtgcac gcacgtacgc gcacaggtgt tcaagtgcac
    10621 cgcggcgaat ggcgcggtga gctaccagga ccatgcttgc gggcaccacg aatcgcagac
    10681 ggtgatgaag gtgccgggcc agtcgccgcc gaactacgtg ccgcctgcgc cggccagctc
    10741 gccgcagccg gcatcgacgc cgccactgcc cacaccgatc tacgttccgc ctccgtcgcc
    10801 gttgccggcg atgtacgcgt gcgtgggtgc agtgaacggc gagccatacc tgacgcggtc
    10861 accgcccccg ccgtatctcg cacccctggg cgtgatgggc tatccgccgc ggacgctttc
    10921 ccaggcctac ggcgccccgg gcggcgcggg catgtccgcg ccggagcttt cgaagcctcc
    10981 gatcggtgga cctcccgttg cgaccggcat gaccgaagtg caggactact gcctgccggc
    11041 tacccgtgcg caggtctgcg gattcgtgca gcgagattac gacgagaacc accgcaagct
    11101 gcgcatggcc atgccgcgcg agcagccgcc gtacgaacag cgcgagcgc
//
LOCUS       DASSNH010000102         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1537162, whole genome shotgun sequence.
ACCESSION   DASSNH010000102 DASSNH010000000
VERSION     DASSNH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537162"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFK00_03640"
     CDS             <1..1333
                     /locus_tag="VFK00_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592079.1"
                     /translation="KLMASTSYSTLDSVENVDATPLLALGDQANGQPYGTLETLTIPQ
                     TKATEEIRLTSPSVEHVEWLVGVFATHETGDNKQDIYASDYYTGTPQASPFGIPIGND
                     VQPSTYDAYAVYGSATWHATDRFDLEAGLRYSHDRQHYTEIGVGALFGSPTMITLLDK
                     RSSDSSTTFSLTPQFHISDRTMLYARAASGFLPGGPNVVTVAIPGVPSTFSPTKLTDY
                     ELGLKSTSLDHRLTVDLAAYYINWTKIPLVTFVNPFTFLGNGGEARSNGLEASIQWIP
                     AGGLKLSANAAYNKSTLSKDAPYPSNGKRGDPLPYAPKLTLSLNGDYDFALGGGWHGY
                     VGASYQYIDERSTDYAFSYPVPGVLPPLPSSPTVPGYNTINLRAGFDHDEWSIDAYIK
                     NLTNQRGIVQGSTFQNYVPIAGALNPVTGKMEDNVTIITPRLFGISVSRNF"
     gene            1421..3454
                     /locus_tag="VFK00_03645"
     CDS             1421..3454
                     /locus_tag="VFK00_03645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7592080.1"
                     /translation="MLKRLVSLLFAACICVPCLAAAQTPPPTGPVVPVPASSTPAPTN
                     AAPPAAATATPPTTTPPLDAQDLRTFFDGLVPYMLKRNDIAGGVVAVVKDGQLVFAQG
                     YGYSNVDKRTPVVADQTLFRIGSVSKLFTWTAVLQLVAQGKIDLDTDVNKYLDFRIPE
                     QYGKPITMRDLMTMTPGFAETIKDLFVASPNEVYPLGRYLKNHIPPRIFPPGKVVAYS
                     NYGAALGGYIVQRVSGEPFDTYVEDHILKPLEMSNSTFKQPLPKAMQGDLATGYVTAS
                     GETIVPFEPIVPYPAGSFTSTATDMANFMIAELQGGQFQGASILPPATLQMMHSHHYS
                     AAPGMNGYDFGFYQENRNGLRIIGHAGDTLAFHTDLHLLLDRDVGVYMSFNSAGKAPN
                     GTAESVRTDLFRAFLDRYFPYTLPKQPAVATAKHDAAQVAGWYRASRREDSALRLLFM
                     LGQARVSALPNGDITVNMLKSPSGAVKHWREIGPLLYQQVDGQSRLRFVANPDGSIKW
                     WVTDDFPPVEVFQPVHGLEQHGWFAPLTEAAIAIYILTLLIWIGGAIARRHFRARLVL
                     ASGQFRLRLAARIGVILQLGVVAGWLGLLAWFSQPLSLFKGSFDTWLILLYILGVLGI
                     LGGIAIVLDALWRIVRGPGGLLCRVGEAVLALCAIYGIWAIIAYGLVNFNLHY"
     gene            3477..4862
                     /locus_tag="VFK00_03650"
     CDS             3477..4862
                     /locus_tag="VFK00_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7592081.1"
                     /translation="MECSQADPPAPDSTGAGATVGGYVQQLDRRLKLRHLLVYGMVFI
                     VPIAPVAVYGFVAHASDGMVPLVYLVGIVAMFFTAMSYKQLSAEFPFAGSVYAYVRRG
                     INPFLGFVAGWMILADYLLVPAVIYIFVSNWIGGLAPGVPHWVWIVALLAVNTGINLL
                     GVRLQSQAHFILLALELVALAIFVALAIRFVFVLGHGAGGFSWAPLYQPGHLSLGFIA
                     TATTIAALSFLGFDAISTLAEESQRPRRDVGTATVLTLLLLGALFVLQTWLAALAHPD
                     YANLDDNLGFFQIAREVGGAALFVLFIVVKALATGVASALASQSAISRILFAMGRDRA
                     LPCGRFLSKVNPRFKTPSNAILFVAALSLVLSLTVPITVLLHLVNFGALTSFLLLNLS
                     VPIYFWKRRGERTRPVRRLVLPVCGLIVVGFIFTGFDRTTFIFGGAWLLAGLLMATFR
                     RQSPLPLEASP"
     gene            4859..>5561
                     /locus_tag="VFK00_03655"
     CDS             4859..>5561
                     /locus_tag="VFK00_03655"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7592082.1"
                     /translation="MKRMWQLLVTLVLACGLGAVGAQVPQPAPRKQATVPATPTPQPH
                     ALTAQDVSTFFSGMLPYMLERGDIAGGAVVVVKDGKVLFARGYGDADLEHHTPVSPAT
                     TLFRIGSVSKLFTWTAVMQLVEQHKLDLDRDINTYLDFRIPPRYGKPITLRELMTHTA
                     GFEDTTQDLLPATPAGVDLGRYLKAHIPARIFPPGKIVAYSNYSAGLAGYIVERVAGE
                     PLDEYIRQHILQPLDM"
BASE COUNT          935 a         1905 c         1697 g         1024 t
ORIGIN      
        1 gaagctgatg gcatccacca gctacagcac cctcgacagc gtcgagaacg tggacgcgac
       61 gccactcctt gccctcggcg accaggccaa tgggcagccg tacggcaccc tggaaacatt
      121 gacgattccc cagaccaagg cgaccgagga aattcgcctg acgtcgccct ctgtggaaca
      181 tgtggaatgg ctcgtgggtg tttttgcgac ccatgaaacc ggcgacaaca agcaggacat
      241 ctatgcatcc gattactaca ccgggacacc gcaggcctcg ccgttcggca ttccgatcgg
      301 caacgacgtg cagccgagca cgtatgacgc ctatgccgtc tacgggagcg cgacctggca
      361 cgccaccgat cgcttcgacc tggaagccgg cctgcgctac agccacgatc gccagcacta
      421 caccgaaatc ggcgtgggtg cgctgttcgg ctcgccgacg atgatcaccc tgctcgacaa
      481 gcgttcctcc gacagcagca ccacgttctc gctcacgccg cagttccaca tcagcgacag
      541 gacgatgctg tatgcgcgtg cggcatcagg cttcctgccg ggcgggccca acgtggtcac
      601 ggtcgcgatc cccggggtac cgtcgacctt cagcccgacc aagctgaccg attacgagtt
      661 gggcctgaaa tcgacctcgc tggatcaccg gctgacggtg gacctggcgg cgtactacat
      721 caactggacc aagatcccgc tggtcacctt cgtcaatccg ttcacgttcc tcggcaacgg
      781 cggcgaggcc aggagcaacg gactggaagc ctcgatccag tggatcccgg cgggcggtct
      841 caaactgtcg gccaacgccg cctacaacaa atccacgttg agcaaggacg ccccgtaccc
      901 gtccaatggc aagcgcggcg atccgctgcc ctacgcgccg aaactcaccc tcagcctcaa
      961 cggggattac gacttcgccc tgggcggcgg ctggcacggc tacgtgggcg cgagctacca
     1021 gtacattgac gaacgctcga ccgactacgc gttcagttac ccggtcccgg gtgtcctgcc
     1081 accgctcccg tcgagtccga ccgttccggg ctacaacacc atcaacctgc gtgccggctt
     1141 cgaccacgat gagtggagca tcgatgccta catcaagaac ctgaccaacc agcgcggcat
     1201 cgtgcagggc agcacgttcc agaactacgt gccgatcgcc ggagcgctca atccggtcac
     1261 ggggaaaatg gaagacaacg tcacgatcat cacgccccgc ctgttcggca tctcggtcag
     1321 caggaatttc tgagcgcgcg tgcgtcttca tccagccgcg gcggtggagc ccggccgcag
     1381 tgatgtcgca tccaattcac cctttattcg gggaaacgcc atgctgaagc gtctcgtctc
     1441 gctgctgttc gccgcttgca tttgcgtgcc gtgcctggcc gctgcgcaaa cgcctccccc
     1501 caccgggccg gtcgtacccg tgccggcgtc gtcgacgccc gcgcccacca atgctgcgcc
     1561 acccgcggcc gctacagcca cacctccgac gaccacgccg ccgctggacg cgcaggacct
     1621 gcgcacgttc ttcgatggct tggtgccgta catgctgaag cgcaatgaca tcgcgggcgg
     1681 cgtggtggcg gtcgtgaagg atggccagct cgtcttcgcg caaggctacg gctactcgaa
     1741 cgtcgacaag cgcactcccg tcgtggcgga ccagaccctg ttccggattg gctcggtgtc
     1801 caagctgttc acctggaccg cggtgctgca actggtcgcc cagggcaaga tcgatctcga
     1861 taccgacgtc aacaagtacc tggatttcag gattcccgag caatacggca agccgatcac
     1921 gatgcgcgac ctgatgacca tgacgcccgg cttcgccgag acgatcaagg acctgttcgt
     1981 cgcgtcaccg aacgaggttt acccgctcgg gcgctatctc aagaaccata tcccgccgag
     2041 gatcttcccg ccggggaagg tggtggcgta ttcgaactac ggcgccgcgc tgggcggcta
     2101 catcgtgcag cgcgtttcgg gcgagccgtt cgatacctac gtcgaagacc acatcctgaa
     2161 accgctggag atgtcgaact cgacattcaa gcagccgctg ccgaaagcca tgcaaggcga
     2221 tctcgccacc ggctacgtca ccgccagcgg cgagacgatc gtgccgttcg agccgatcgt
     2281 gccgtatccg gcaggctcgt tcacctccac tgccacggac atggcgaact tcatgatcgc
     2341 ggaattgcag ggcggccagt tccagggcgc ctcgatcctg ccgcccgcga ccctgcaaat
     2401 gatgcactcg caccactaca gcgccgcgcc cggcatgaac ggctatgact tcggtttcta
     2461 ccaggaaaac cgcaacggcc tgcgcatcat cggccacgcg ggcgacaccc tcgccttcca
     2521 taccgacctg cacctgctgc tcgacaggga cgtcggcgtg tacatgtcat tcaacagcgc
     2581 cggcaaggca cccaacggca ctgccgagag cgtacgcacc gacctgttcc gcgcgttcct
     2641 cgatcgctac ttcccctaca cattgccgaa gcagccggcc gtcgcgacgg ccaagcacga
     2701 cgccgcacaa gtcgccggct ggtaccgggc gagccggcgc gaagacagcg cgctgcgact
     2761 gctgttcatg ctcgggcagg cgcgggtatc cgcgctgccc aatggcgaca ttaccgtcaa
     2821 catgctgaaa agcccgtccg gtgcggtcaa gcattggcgt gaaatcgggc cgctcctcta
     2881 ccagcaggtg gacggccagt cgcgcctgcg ctttgtcgcg aacccggacg gttcgatcaa
     2941 gtggtgggtc accgacgact ttccccctgt cgaggtgttc cagccggtgc acggcctcga
     3001 acagcacgga tggttcgcgc cactcaccga agccgcgatc gcgatctaca tcctgaccct
     3061 gctgatctgg atcgggggcg ccatcgcgcg ccgccacttc cgcgcgaggc tggtgctggc
     3121 ttccggccaa ttccggttgc ggctggccgc gcggatcggc gtgatcctgc aactcggtgt
     3181 cgtggcgggc tggctgggac tgctggcctg gttttcgcag ccattgtcgt tgttcaaagg
     3241 cagcttcgat acctggctga tcctgctgta catcctcggc gtgttgggga tcctcggcgg
     3301 catcgcgatc gtcctggatg cgctgtggcg gatcgtgcgc ggcccgggcg gcctgctgtg
     3361 ccgggtcggc gaagcggtgc tcgccctgtg cgcgatctac ggcatctggg cgatcattgc
     3421 ctatggcctc gtcaacttca atctccatta ctgacgggaa ccgccggatc gtgcagatgg
     3481 aatgcagcca ggctgatccg cccgcgccgg attccaccgg cgcgggcgcg accgtcggcg
     3541 gctacgtgca gcaactggac cgccgcctga agttgcggca cctgctggtg tacggaatgg
     3601 tgttcatcgt tccgatcgcg ccggtcgcgg tgtacggctt cgtcgcgcac gccagcgatg
     3661 gcatggtgcc actggtgtac ctggtcggca ttgtcgcgat gttcttcacc gcgatgagct
     3721 acaagcagtt gagcgcggag ttcccgttcg cgggatcggt gtatgcctac gtgcggcgcg
     3781 gcatcaaccc gttcctcggc ttcgtcgccg gctggatgat cctggccgat tacctgctgg
     3841 ttccggccgt gatctacatc ttcgtctcga actggatcgg cggcctcgcg cccggcgtgc
     3901 cgcactgggt gtggatcgtg gcgctgctgg cggtcaacac cggcatcaac ctgctgggcg
     3961 tgcgcctgca atcgcaggcc catttcatcc tgctcgcgct cgaactggtg gcgctggcga
     4021 tcttcgtggc gctcgccatc cgtttcgttt tcgtgctcgg ccacggcgcc ggcggattct
     4081 cctgggctcc gttgtaccag cccggccact tgagcctggg tttcatcgcg accgccacaa
     4141 ccatcgcggc ccttagcttc ctcggcttcg atgcaatcag cacgctcgcc gaggaatcgc
     4201 aacggccgcg acgcgacgtc ggcacggcca cggtattgac gctgctgctg ctcggcgcgc
     4261 tattcgtgct gcaaacctgg cttgcggcgc tcgcgcatcc ggactacgcc aacctggacg
     4321 acaatctcgg gttcttccag atcgcgcgcg aggtcggcgg cgccgcgctg ttcgtgctgt
     4381 tcatcgtcgt gaaggcgctg gcgaccggcg tcgccagcgc actcgcctcg cagtcggcga
     4441 tctcgcggat cctgttcgcg atgggccgcg atcgcgccct gccttgcggc cggttcctgt
     4501 cgaaggtcaa cccgcgcttc aagacgccct cgaacgccat cctcttcgtc gccgcgttgt
     4561 cgctggtgct ctcgctgacg gtgccgatca cggtgctgct gcacctggtg aatttcggcg
     4621 cactgacgtc gttcctgctg ctgaacctgt cggtgccgat ttatttctgg aagcgacgcg
     4681 gtgagcgtac ccgccccgtg cgccggctcg tgctgcccgt ttgcgggctg atcgtcgtcg
     4741 gattcatttt caccggtttc gatcggacga ccttcatttt tggcggcgcg tggctcctgg
     4801 ccggactgct gatggccacg ttcaggcgcc aaagcccact tccgctcgag gcctcgccat
     4861 gaagcgcatg tggcaactcc tggtgacact cgtgctggct tgcggtctcg gtgcggtcgg
     4921 cgcgcaggtg ccgcaacctg cgccgcgcaa gcaagcgact gttccggcaa cgcccacgcc
     4981 gcagccgcac gcgctgaccg cgcaggatgt ttccacgttc ttcagcggaa tgctgccgta
     5041 catgctggag cgcggcgaca tcgcgggggg cgcggtcgtc gtggtgaagg atggcaaggt
     5101 attgttcgcg cgaggctatg gcgacgccga cctggaacac cacacgccgg tgtcgccggc
     5161 caccacgctg ttccgcattg gctcggtgtc caagctgttc acctggacag ccgtgatgca
     5221 acttgtcgag cagcacaagc tcgacctcga ccgcgacatc aacacctatc tcgatttcag
     5281 gatcccgccg cggtacggca agccgatcac cctgcgcgag ctgatgacgc acacggccgg
     5341 cttcgaggac acgacgcagg acctgctgcc ggcgacgccc gcaggcgtgg acctcgggcg
     5401 gtatctcaag gcacacatac cggcccgcat cttcccgccc ggcaagatcg tcgcgtactc
     5461 caattacagc gccggactcg ccggctacat cgtggagcgc gtggcggggg aaccgctcga
     5521 cgaatacatc cggcagcaca tcctccagcc gctggacatg c
//
LOCUS       DASSNH010000103        16548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_397379, whole genome shotgun sequence.
ACCESSION   DASSNH010000103 DASSNH010000000
VERSION     DASSNH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 16548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16548
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397379"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="tal"
                     /locus_tag="VFK00_03660"
     CDS             complement(<1..1048)
                     /gene="tal"
                     /locus_tag="VFK00_03660"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7592083.1"
                     /translation="MNPTKQLHDLGQSIWLDNITRGMLDDGTLQGYIDEYHVTGLTSN
                     PSIFDKAISGGSDYDDAIGELLAEGKSGQDLFFELALQDLRRAADLFKPAFHASDGKD
                     GFVSLEVSPLLADDTRGSIESARVLHDKAQRDNLFIKIPGTPAGLPAIEESIYRGIPI
                     NVTLLFSREQTLAASEAWMRGIERRIGEGLDPDVDSVVSLFVSRWDVAVADKVPADLK
                     DRLGIAISQRTWKAWQEFREAPRWKKLIAAGAHPQRLLWASTGTKDPKAPDTLYVEAL
                     AAPDTINTMPDKTLLAFADHGKLHGPMADDGGDCEKVIAEFNAAGIDDAVLAGQLQKE
                     GAEKFVKSWNDLLGK"
     gene            complement(1054..1752)
                     /locus_tag="VFK00_03665"
     CDS             complement(1054..1752)
                     /locus_tag="VFK00_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET7592084.1"
                     /translation="MSAQPDLVFLFDVDNTLLDNDAVYADIDRHLKQVFGAEAAKAYW
                     GIYEDLRAELGYADYLGAVQKFRLVCGDEPRAQQLAAFLLDYNFAQRLFPGALDAIRH
                     LSRFGPCVVLSDGDVVLQPRKISVSGIADAVEGRVLIYVHKERMLEDVSRRHPARHYV
                     MVEDKLRVLDAMKRRWRDRLTTVFVRQGHYANDPAARREYPAAQIELAGIAELRTLQA
                     EAFLSPMPSATATE"
     gene            complement(1749..3137)
                     /gene="zwf"
                     /locus_tag="VFK00_03670"
     CDS             complement(1749..3137)
                     /gene="zwf"
                     /locus_tag="VFK00_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7592085.1"
                     /translation="MTAATSDAFVFFGATGDLAYKQVFPALQALIQEDHFDLPIIGVA
                     RSDWNLERLRERARASLRDHGGIDEKAARKLCRLLRFVNGDYNAPDTFTELRKALGEA
                     KHPLHYLAIPPSMFETVVKGLDASGCAEGARVVVEKPFGRDLKSAQALNRTLHRIFPE
                     DAIFRIDHYLGKEAVQNLLYFRFANSFLEPVWNRTYIHSVQITMAEAFGVEGRGGFYE
                     EVGALRDVVQNHMLQVLVLLAMDAPTGRNGESMRSEKQRLLRAIRPLDPREVVRGQFD
                     GYRKEHGVARNSRVETFAALKLHIDTWRWAGVPFYIRAGKEMPVTCTEVFVDLKCPPL
                     NVFDKITGEQSNYFRFRLSPDVVISTGARVKKPGEAMRGENVELVARSQPRRVRLPYT
                     RLLGDALRGDAELFGRDDALEAAWRIIDPALNDAEPVERYRSRSWGPGAAEKLIAPDG
                     GWHNPVVKKSGR"
     gene            complement(3150..6911)
                     /locus_tag="VFK00_03675"
     CDS             complement(3150..6911)
                     /locus_tag="VFK00_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET7592086.1"
                     /translation="MLAEAQSLATNVHLLGNDEYAVMLAASGAGYSHWKGRAVTRWQA
                     DQVGGEQGAYVYVRDIDGAALWSATPQPTGGAVRYWFDEAMARFMRRDGALTTTLEVA
                     VDPDHAVEVRGVGLRNDGTVERQVDVTSYLELVLGSAPTDAAHPAFSKMFVQTTIEEG
                     VLLAWRRKRDASDPGIWAAHALVVDGDTGGEREHETDRACFIGRDGSLSDPAALQPGA
                     RLSGTTGTVLDPIFSLRVGLTVGAGETRRLAFVTAAAESREEVLQLIARYADAGACAE
                     IFARAQREPRSRGAGDLTPAQAALCQRLAGALVYDDPTLRPAREVIAKGKGGAPVLWA
                     AGISGDLPIVVARIAQPEETGLARDLLGAQAFWRDRQLPADLVLLDVAKAAEADAVEA
                     ALKEIAPVGEQQDKARGSVFVLRADRLDPGLLDGLMASARIVLDGRDGGLASQSKCAA
                     VASGAGDSAPPAAGKPRVRGGRPAAMPKPAELDFWNGLGGFTKDGREYVVALQRGDTT
                     PVPWSHVVANPGFGFLVTATGGGYTWAINSQQNQITPWSNDAVCDPPGDCLLLRDADD
                     GESWSATAAPVRAGDATYVARFGPGYARFDANVHGIESELVQCVAATDPVKLSRLRLH
                     NRGRRPRRIAVAAVANWRLGPIGHDPRPTTQVEADAARGAVFARNAARAEFADETAFV
                     ACCAEGAQPTSDRNLRSAAVVVVELAPGADRELVFLLGEGSDRAAAERLVDRYRECDF
                     DGVLAETGKTWNSVLGPLQVETPQHSLDLLVNRCLPYQVLACRLWARTGFYQASGAFG
                     FRDQLQDVGALCIARPDLAREHILLTASRQFEEGDAQHWWLPPSGKGVRTRIVDDRLW
                     LPFTVAHYVATTGDEAILATDVPFLHGDVLATGQSDAFFQPQRSAVSDTLFEHCARAI
                     DTSLATGVHGLPLIGTGDWNDGMNRVGAGGKGESVWMAWFLCKVIADFAPCAEARGDP
                     RAARWRDHVRALQLAAETKAWDGAWYRRAFYDDGTPLGTSADTECHVESMAQSWAVIS
                     GAGDPDRARAAMRAVNEQLVRPRDGLVALFTEPFDQTPHDPGYIKGYPPGLRENGGQY
                     THGSIWSLVAFAMLGDGDKAGELLEIFDPIRKSATPESMARYKVEPYVECADVYSVAP
                     HLGRGGWTWYTGSAGWLYRAIVEWVLGIRVRSDRLLLEPCIPRQWKGYAFTYRRGGTE
                     YRVEVENPGHVCGGIASVAVDGVRLQDPAVGIELQDDGQTHHVHATLQT"
     gene            7070..7882
                     /locus_tag="VFK00_03680"
     CDS             7070..7882
                     /locus_tag="VFK00_03680"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HET7592087.1"
                     /translation="MAETEAQPAAEAAAQGASAPATTSPDATPRLTCYRVDPRAVPII
                     PGRRERMWMDQTADHYAYRCLPLSMANASGWELTSPFAFEVTWDGNQAIEGITARAQG
                     LDPQLLRAFITSHFGHGILTFHTGWLFRTSPGWGLWVRGAPNYAKDGIHPLDGMVETD
                     WLPFPFTMNWRFTRPCTVRFGTGDPFCFITLCPHAVLDDITPTRANIEDDPGLKADYT
                     EWAASRAEFNKLLHDGDPATVARKWQRGYFQGKGTGGEAPFHVSKRRLKPVE"
     gene            complement(7944..9737)
                     /locus_tag="VFK00_03685"
     CDS             complement(7944..9737)
                     /locus_tag="VFK00_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HET7592088.1"
                     /translation="MGSDDVLPLPRQTRAFIVLVAMVQALLLYAAWSAQEHLWGWLSL
                     RGTQVYWYTLILAVPTMMTLSVRRLDDRRFWQHAAAVAIIYALLASWAAWSVTGAPGI
                     NAESVLLPFGITLGGALFVALPFLQCRQEQGHWRAPYADLFDHAWQNGLTLAVALLFT
                     GLCFVVLRLWAELFALVHIRFFRDLFFDTTSFVFLATGLFAGLGILIGRTQHHPLRIA
                     RQILFAVCRGLLPLLALIALMFLASLPFTGLSPLWATRSAAALLLGLLLLLVAFVNAV
                     WQDGVDTRPYPRVLRWLIDAALVLSPIYATLALYALALRIGQYGWTDQRFWAVLVAVV
                     LACHAVGYAAAAIHRGSTWLGWLPRVNVALAGIVVLLAVLANSPALDPYRISVASQVG
                     RLHAGRVAPAKLDVAELRFDNGRRGYRAAVALQQDPKMRADRSALAALTRTIARQQQE
                     RPLTDAEQKRLSVTTVAALRAQIAVAPGSEAPDEDWYRNLLSAKYGHSCRLRGADCVL
                     LSADLRGDGQSEILLCDLGMSELVTCQLWMRDVHGQWQWRSEGSLQLSPDDSAKGAAV
                     QRALRAGKLEVEWPHWPDVKVAGVKGRIMSN"
     gene            9863..10768
                     /gene="gnd"
                     /locus_tag="VFK00_03690"
     CDS             9863..10768
                     /gene="gnd"
                     /locus_tag="VFK00_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET7592089.1"
                     /translation="MQLGMIGLGKMGNFMAQRLMKGGHDVVGFDPSDKARKALVDAGG
                     EAVDSLAALVRALEPPRAVWVMVPAGKIVDETIAGLAAVLGEGDVVIDGGNSNYKDDQ
                     RHATQLGEGGIHFVDCGTSGGVWGLAEGYSMMVGGDAQVVDRLRPIFEVLAPGKDKGW
                     GHVGPVGSGHFVKMVHNGIEYGLMQAYAEGFSILKHKQEFELDLQQIAEIWRYGSVVR
                     SWLLDLTADALKKDPDMKDIAPYVSDSGEGRWTVAEAIDLDVPAPIITASLIERLRSR
                     DTDSFSDKLLSAMRNQFGGHAMKKE"
     gene            10774..11484
                     /gene="pgl"
                     /locus_tag="VFK00_03695"
     CDS             10774..11484
                     /gene="pgl"
                     /locus_tag="VFK00_03695"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7592090.1"
                     /translation="MPPALPAQVVRWHEFPDDVSLLADAQSRILGAARNAIARRQAFD
                     IVLAGGNTPRKLYRALRRADADWARWHVWFGDERCVPADDPGRNSRMARSGWLDHVAL
                     PAANVHVIPAERGAREAAALYARELRAADGFDLVLLGLGEDGHTASLFPGRDWGVHED
                     SPDALAVFDAPKPPPERVSLSTHRLSNASNVLFLVEGAGKRDAVAAWKQGEAIPAASI
                     APEAGVDVLLDAAASGTA"
     gene            complement(11661..12221)
                     /gene="orn"
                     /locus_tag="VFK00_03700"
     CDS             complement(11661..12221)
                     /gene="orn"
                     /locus_tag="VFK00_03700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET7592091.1"
                     /translation="MSGSASENLIWIDLEMTGLCPDSDSIIEIATVVTDRDLHVLAEG
                     PHTAIRHEEARLLAMDDWNRNQHGKSGLWQRVLESGVDHAQAEAETLEFLDAWVTPGK
                     SPMCGNSICQDRRFLHRQMPSLERFFHYRNLDVSTLKELARRWAPEIYKGFSKDSAHT
                     ALSDIRDSIDELRYYRAHMGELAGPR"
     gene            12305..13783
                     /gene="sbcB"
                     /locus_tag="VFK00_03705"
     CDS             12305..13783
                     /gene="sbcB"
                     /locus_tag="VFK00_03705"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HET7592092.1"
                     /translation="MPAPTLLWYDYETTGTDPARDRPVQFAAIRTDTDLRPLGDPDVR
                     YCRPAPDVLPHPEACLVTGIAPQDAEREGLVEAEFAAAVHERMVEPGTCSTGYNSIRF
                     DDEVNRNLFYRNFFDPYEHVWKNGNSRWDIMDLARACYALRPAGIEWPVREDGAPSFK
                     LEHLAAANHLGQRRAHDALSDVEATIALARLLRDRQPRLYDWCFALRDKNTASPKLAA
                     ALPTLQPLLHVSGRYAPARGCLAMVVPLAEHPTRAGTFIVADLDVDPDGWIDLDPTDL
                     AERMFTRRVDLPEDIDRPPLKEIHANRSPFLAPIETLRNVDVVRICLDPPRCLRHLER
                     IRASVGLAERVRSAFAGRVAAWTDRNDPELAMYSGFPSPADKARCDRVRATPPAQLHS
                     TDFGFGDPRYPELLFRYRARNWPETLDAGERERWSAFVRDKLTCDNGLGRLTLQQYFD
                     VIARLRAEVPPGPKQALLDRLQDWGETVRQEFELREGTGQLV"
     gene            13826..14512
                     /locus_tag="VFK00_03710"
     CDS             13826..14512
                     /locus_tag="VFK00_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HET7592093.1"
                     /translation="MPQAYFSPATFRFLRALDRNNNRTWFKAHQPGYEREVREPFLQL
                     IADLQVPVAKISTHYRADPRKIGGSLFRVQRDTRFSHDKAPYKPWAGARLFHERRREI
                     AAPSFYLHIAPGDCFIGAGLWHPENHTLRNVRNFIADNPAAWQRAAHGRAFRSRYAFR
                     GESLIRPPHGFPPDHPLIEDLKRKSFAVGTGFDDTLACSPKLLPFVSDHFRRLAPLVD
                     YLCAAQNLEF"
     gene            14910..15308
                     /locus_tag="VFK00_03715"
     CDS             14910..15308
                     /locus_tag="VFK00_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592094.1"
                     /translation="MLGTYQVAPEASQAIVAAESRSAQRENMPYIAVSQRLNEGRAQF
                     QHADWFAKAGHPGHDLVLLEAVLLYEQLYGTAPPAKALEVRAPMFVPGSKFAPPAPVS
                     LPLLPEVALAGGYDYSSTEMANAIELAGSH"
     gene            complement(15490..16449)
                     /gene="epmA"
                     /locus_tag="VFK00_03720"
     CDS             complement(15490..16449)
                     /gene="epmA"
                     /locus_tag="VFK00_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HET7592095.1"
                     /translation="MTALAAKCEAIRLRSRLNALIRDFFAHRGVAEVETPILSAAGNT
                     EPNIAGFVTGFAGPIAAGPRTRYLRTSAEYPMKRLLASGFGDCYELGRVFRNGEAGSS
                     HNPEFTMLEWYRVGFEHRRLALETAQLVRVALALVQRDAPVVTTSYRDWFRDHLRLDP
                     FEAGEGALRAPLAEFRIESSGLGRDDWLDLLVTHRLQPALPGGGITIVYDFPASQCSL
                     ARIAGNPPVAERFEIYLGQHELANGYHELNDAAEQRARFEHENLRRREHGLDELPLDE
                     NLLAVLDHLPDCAGVALGVDRLLMQLLGTDDIRDVIAFPFAEA"
BASE COUNT         2724 a         5735 c         5467 g         2622 t
ORIGIN      
        1 gcttgcccag caggtcattc caggacttga cgaacttttc ggcgccttcc ttctgcaact
       61 gcccggcgag caccgcgtcg tcgatgccgg ccgcgttgaa ttcggcgatc actttttcgc
      121 agtcgccacc gtcgtccgcc atcggtccgt gcagcttgcc gtggtcggcg aacgcgagca
      181 gcgtcttgtc gggcatcgtg ttgatggtgt cgggcgcggc aagcgcctcg acgtacagcg
      241 tgtcgggggc cttcggatcc ttggtgccgg tgctggccca cagcaggcgc tgcggatgcg
      301 cgccagcggc gatcagcttc ttccagcgcg gcgcttcgcg gaattcctgc cacgccttcc
      361 aggtgcgctg gctgatcgcg atgccgagcc ggtccttgag gtcggccggc accttgtcgg
      421 ccaccgcgac gtcccagcgg ctgacgaaca gcgataccac cgaatcgacg tcgggatcga
      481 gcccctcccc gatgcggcgc tcgattccgc gcatccaggc ctcggacgcc gcgagcgtct
      541 gctcgcgcga gaacagcaag gtcacgttta tcgggatgcc gcggtagatc gactcctcga
      601 tcgccggaag cccggctggc gtgcccggga tcttgatgaa caggttgtca cgctgcgcct
      661 tgtcgtgcag cacgcgcgcg gattcgatgg atccccgggt atcgtcggcc agcagcggcg
      721 aaacctcgag cgacacgaat ccgtccttgc catcgctggc atggaacgcc ggcttgaaga
      781 ggtccgcggc gcggcgcagg tcctgcagcg ccagttcgaa gaacaggtcc tggcccgact
      841 tcccttcggc gagcagttcg ccgatcgcgt cgtcgtaatc gctgccgccc gagatcgcct
      901 tgtcgaagat cgacggattg gaggtgaggc ccgtgacgtg gtactcgtcg atgtagccct
      961 gcagcgtgcc gtcgtcgagc atgccgcggg tgatgttgtc gagccagatg ctctggccga
     1021 ggtcgtgcag ttgcttggtc gggttcatgg gattcactcc gtggcggttg ccgatggcat
     1081 gggggaaagg aacgcctccg cctgcagcgt gcgcagctca gcgatgccgg caagctcgat
     1141 ttgcgcggcc gggtattccc ggcgcgcggc gggatcgttg gcgtaatggc cttggcggac
     1201 gaagaccgtg gtcaggcgat cgcgccatcg ccgcttcatc gcgtcgagga cccgcagctt
     1261 gtcctcgacc atcacgtagt gcctggccgg atggcggcgg gacacgtcct ccagcatgcg
     1321 ttccttgtgg acgtagatca ggacccggcc ttcgaccgcg tcggcgatgc cggatacgct
     1381 gatcttgcgc ggctgcagca ccacgtcgcc gtcggacagc accacgcagg gcccgaaccg
     1441 cgagagatgc ctgatcgcat ccagcgcgcc gggaaacagg cgctgcgcga agttgtagtc
     1501 gagcaggaac gcggcgagct gctgggcccg cggttcgtcg ccgcacacca gccggaattt
     1561 ctggacggca ccgaggtaat cggcgtagcc cagttccgcg cgcaggtcct cgtagatgcc
     1621 ccagtacgcc ttcgcggcct ctgcaccgaa tacctgcttt aggtgccggt cgatgtcggc
     1681 atacacggca tcgttgtcga gcagtgtgtt gtcgacgtcg aagaggaata ccaggtcggg
     1741 ctgcgcactc atcgaccgct cttcttcaca accgggttgt gccatccgcc atccggtgca
     1801 atcaacttct ccgccgcgcc gggaccccag ctgcgggatc gataacgctc cacgggctct
     1861 gcatcgttca aggctggatc gatgatgcgc catgcggctt ccagggcgtc gtcgcggccg
     1921 aacagttccg cgtcgccacg cagcgcatca cccaggagtc gggtgtacgg cagccgcacg
     1981 cgccggggtt gcgaacgcgc gaccaactcg acgttttcgc cgcgcatggc ctcgcctggc
     2041 ttcttgacgc gagcgccggt ggaaatcacc acgtcgggac tcagccggaa gcggaaatag
     2101 ttggactgct ccccggttat cttgtcgaac acgttcaacg gggggcattt gagatccacg
     2161 aacacctcgg tgcaggtcac cggcatttcc ttgccggcac ggatatagaa cggtacgccg
     2221 gcccagcgcc acgtatcgat gtgcagcttc agtgccgcga acgtctcgac cctggagttg
     2281 cgtgcgacgc catgttcctt tcgatatccg tcgaactggc cgcgcacgac ttccctggga
     2341 tcgagcggac ggatcgcgcg cagcagccgc tgcttttcgc tgcgcatgga ttctccgttg
     2401 cggccggtcg gcgcatccat cgcgagcaac accagcacct gcagcatgtg gttttgcacc
     2461 acgtcacgca gcgcgccgac ttcctcgtag aacccgccac gcccctccac gccgaatgcc
     2521 tcggccatgg tgatctggac gctgtggatg taggtgcgat tccacaccgg ttccaggaag
     2581 ctgttcgcga agcggaagta caacaggttc tgcaccgcct ccttgccgag ataatggtcg
     2641 atgcggaaga tcgcgtcttc cgggaatatc cgatgcaacg tgcggttcag tgcctgcgcg
     2701 gatttgaggt cgcggccgaa cggcttctcc accaccaccc gcgcgccctc cgcgcagccc
     2761 gaagcgtcca atcccttgac caccgtttcg aacatgctgg gcgggatcgc gaggtaatgc
     2821 agcggatgct tcgcttcacc cagcgccttg cgcaattcgg tgaaggtgtc cggagcgttg
     2881 tagtcgccat tgacgaaccg cagcagccgg cacaacttgc gcgcggcctt ctcgtcgatg
     2941 ccaccgtggt cgcgcaggct ggcgcgggca cgctcgcgca ggcgctccag gttccagtcc
     3001 gaccgcgcca cgccgatgat cggcaggtcg aaatgatctt cctggatcaa cgcctgcagt
     3061 gccgggaaaa cctgcttgta ggcgaggtcg ccggtggcgc cgaagaacac gaatgcgtcg
     3121 gacgttgccg cggtcatcga tgggaatcct caggtctgca aagtggcgtg gacgtggtgg
     3181 gtttgcccgt cgtcctgcag ctcgatgccc acggcgggat cctgcaaccg tacaccatcg
     3241 accgccacgc tggcgatgcc gccgcaaacg tggccggggt tttccacctc gacccggtat
     3301 tccgtcccgc cgcgccggta cgtgaatgcg taccccttcc attgccgcgg aatgcagggc
     3361 tcgagcaaca ggcggtcgct ccggacgcgg atgccgagga cccattccac gatcgcgcgg
     3421 tacagccagc cggccgaacc cgtgtaccag gtccagccac cgcggccgag gtggggggcg
     3481 accgaataga cgtccgcgca ctcgacgtag ggttcgacct tgtagcgcgc catcgactcc
     3541 ggcgttgccg acttgcggat cggatcgaag atctccagca gctcgccggc cttgtcgcca
     3601 tcgccgagca tcgcgaacgc gaccagcgac cagatcgagc catgggtgta ctgcccgccg
     3661 ttctcgcgca ggccgggcgg atagcccttg atgtaacccg ggtcgtgcgg cgtctggtcg
     3721 aacggttcgg tgaacagcgc gaccagcccg tcgcgcgggc gcacgagttg ctcgttgacc
     3781 gcacgcatcg ccgcccgcgc gcggtccgga tcgccggcgc cgctgatcac cgcccaggac
     3841 tgcgccatgg attcgacatg gcattcggtg tccgccgagg ttcccagtgg cgtgccgtcg
     3901 tcgtagaacg cgcggcgata ccaggcgccg tcccaggcct tggtttcggc ggcgagttgc
     3961 agcgcgcgga cgtggtcgcg ccagcgggcg gcgcgcggat cgccacgcgc ttcggcgcaa
     4021 ggcgcgaagt cggcgatgac cttgcacagg aaccacgcca tccacacgct ctcccctttg
     4081 ccgccggcgc cgacccggtt catgccgtcg ttccagtcgc cggtgccgat cagcggcagg
     4141 ccgtgcacgc cggtggcgag gctggtgtcg atcgcgcgcg cgcagtgctc gaacagggtg
     4201 tccgaaacgg ccgagcgctg cggctggaaa aacgcgtcgc tctgtccggt cgcgagcacg
     4261 tcgccgtgca ggaacggtac gtcggtggcg aggatcgctt cgtcgccggt ggtcgcgaca
     4321 tagtgcgcca ccgtgaacgg cagccacagc cggtcgtcca cgatccgcgt gcgcacgccc
     4381 ttgccgctcg gcggcaacca ccagtgctgg gcgtcgcctt cctcgaactg ccgcgaggcc
     4441 gtcaacagga tgtgttcgcg cgcaaggtcc ggccgcgcga tgcacagcgc accgacgtcc
     4501 tgcaactggt cgcggaaacc gaaggccccg ctcgcctggt agaacccggt gcgtgcccac
     4561 agccggcagg cgagcacctg gtacggcagg caacggttga ccagcaggtc gagcgaatgc
     4621 tgcggcgttt cgacctgcag cggcccgagc acgctgttcc aggttttgcc ggtctccgcg
     4681 agcaccccat cgaaatcgca ttcgcggtaa cggtcgacga gccgctccgc cgccgcgcga
     4741 tcgctgccct cgcccagcag gaacaccagc tcgcgatcgg cgccgggtgc gagttcgacc
     4801 accacgaccg cggcactgcg caggttgcgg tcgctcgtcg gctgcgcgcc ctcggcgcag
     4861 cacgcgacga acgccgtttc gtcggcgaat tcggcacgcg cggcgttgcg cgcgaagacc
     4921 gcgccgcgtg ccgcgtcggc ttcgacctgg gtggtcggcc gcggatcgtg gccgatgggc
     4981 ccgagccgcc agttggcgac ggccgcgacc gcgatccggc gcggccggcg tcctcggttg
     5041 tgcagccgca ggcgcgacag cttgaccggg tcggtcgccg ccacgcactg cacgagctcc
     5101 gattcgatgc catggacatt ggcatcgaag cgcgcgtagc ccgggccgaa ccgcgccacg
     5161 taggtcgcgt cgcccgcccg caccggcgcc gcggtcgcgc tccaggactc gccgtcgtcg
     5221 gcgtcgcgaa gcagcaggca atcgcccggc ggatcgcaca ccgcgtcgtt cgaccacggc
     5281 gtgatctggt tctgctggct gttgatcgcc cacgtgtagc cgccgccggt cgcggtcacc
     5341 aggaagccga aaccgggatt cgcgaccaca tgcgaccagg gcaccggcgt cgtgtcgcca
     5401 cgctggagcg cgaccacgta ctcgcggccg tctttcgtga aaccgccgag gccgttccag
     5461 aaatccagtt cggcaggctt cggcatcgcg gcggggcggc cgccgcgcac ccgcggtttt
     5521 cccgccgccg gcggcgccga atccccggcg ccggatgcca cggcggcaca cttcgactga
     5581 ctcgcgagcc cgccgtcgcg gccatcgaga acgatcctcg ccgacgccat gagcccgtcg
     5641 agcaatcccg ggtcgagacg gtccgcgcgc agcacgaaca ccgatccgcg cgccttgtcc
     5701 tgttgttccc cgacgggtgc gatctccttg agcgcggctt cgaccgcgtc ggcctccgcg
     5761 gccttggcga cgtcgaggag caccaggtcg gcaggcaact ggcgatcgcg ccagaacgcc
     5821 tgcgcgccca gcaggtccct tgccagcccg gtttcttcag gttgggcgat ccgcgcaacc
     5881 acgatcggca ggtcgccgga aatgcccgcc gcccacaaca ccggcgcgcc gcccttgccc
     5941 ttggcgatca cctcgcgggc aggacgcaat gtcggatcgt cgtagacaag cgcgcccgcc
     6001 agacgctggc acagcgcggc ctgggcgggg gtgagatcac cagcgccgcg cgagcgcggt
     6061 tcccgctgcg cgcgcgcgaa tatttcagcg cacgccccgg cgtccgcgta acgcgcgatc
     6121 agttgcaaca cttcctcgcg cgattccgcg gccgcggtga cgaacgccag ccggcgcgtc
     6181 tcgccggcgc ccacggtcaa gccaacgcgg aggctgaaga tcgggtcgag gacggtgccg
     6241 gtcgtccccg acaagcgtgc gccgggttgc agcgccgcgg ggtcggacag cgagccgtcg
     6301 cggccgatga agcaggcgcg atcggtttcg tgttcgcgtt cgcccccggt gtccccgtcg
     6361 acgaccaggg cgtgcgcggc ccagatgccg gggtcggagg cgtcgcgctt gcgccgccac
     6421 gccaacagca cgccttcctc gatcgtggtc tgcacgaaca tcttggaaaa cgccgggtgc
     6481 gctgcatcgg tcggcgcgga acccaggacc agctccaggt aagacgtcac gtcgacctgg
     6541 cgttcgaccg ttccgtcgtt gcgcaggccg accccgcgga cttccaccgc gtggtccgga
     6601 tcgaccgcga cctcgagcgt ggtggtcagc gcgccatcgc ggcgcatgaa gcgcgccatc
     6661 gcctcgtcga accagtaccg caccgcaccg ccggtcggtt gcggcgtcgc cgaccacagc
     6721 gcggccccgt cgatatcgcg gacatacacg tacgccccct gctcgccgcc gacctggtcg
     6781 gcttgccagc gggtgaccgc gcggcccttc caatgactgt agccggcgcc ggacgccgcc
     6841 agcatcacgg catattcgtc attgccaagc agatggacat tcgtcgcgag cgactgggct
     6901 tcggcaagca tggtcgaggg ctcccgcttc ctccgcgtca acgcgcgatt atcgcgccgc
     6961 ccacatcaat cgttcgtgaa acgcgagcct tgaccgttcc cgcgaaacgc cggacgatgg
     7021 ggcgcccggc atcgccgcca tggcgagccg cggtcacgga ggagcctcga tggccgaaac
     7081 cgaagcgcaa ccggccgcgg aggcggcggc ccagggcgca tccgcgccgg cgacgacatc
     7141 gcccgatgcc accccgcggc tgacgtgcta tcgcgtcgat ccgcgcgcgg tgccgatcat
     7201 cccggggcgc cgcgagcgca tgtggatgga ccagaccgca gaccattacg cgtaccggtg
     7261 cctgcctttg tcgatggcca acgccagtgg ctgggagttg acgtcgccgt tcgccttcga
     7321 ggtcacctgg gatggcaacc aggccatcga gggcatcacc gcgcgcgcgc agggcctcga
     7381 tccccagctg ctgcgcgcgt tcatcacctc gcacttcggc cacggcatcc tcaccttcca
     7441 caccggctgg ctgttccgca cctcgccggg ctggggcctg tgggtgcgcg gcgcgccgaa
     7501 ctacgcgaag gacggcatcc accccctgga cgggatggtc gagacggact ggctgccatt
     7561 ccccttcacg atgaactggc gcttcacccg gccctgcacc gtgcgtttcg ggaccggcga
     7621 cccgttctgc ttcatcacct tgtgccccca cgcagtgctc gacgacatta cgccgacgcg
     7681 cgccaatatc gaggacgatc ccggcctcaa ggcagactac acggaatggg ccgcgtcgcg
     7741 cgccgagttc aacaagttgc tgcacgacgg cgatcccgcg accgtcgcgc gcaagtggca
     7801 gcgcggctat ttccagggca agggcaccgg cggcgaagcg ccgttccacg tcagcaagcg
     7861 gcgcttgaag cctgtcgaat gaaaactcga tgtcgttcgg cgaaagaggc ggcacccgcg
     7921 gctgcggctg cgtgatcaca atctcagttc gacatgatcc ggcctttcac cccggccacc
     7981 ttgacgtcgg gccaatgagg ccactcgact tccagcttgc ccgcgcgcag cgctcgctgc
     8041 acggcggccc cttttgcgga atcgtcgggt gacaattgca gcgagccctc cgaacgccat
     8101 tgccattgcc cgtgcacgtc gcgcatccat agctggcacg tgaccaactc cgacatgcct
     8161 agatcgcaca acaaaatctc gctttggccg tcgccccgca gatcggcaga cagaagcacg
     8221 cagtccgcgc cgcgcagccg gcaggaatgg ccgtacttgg cggacaacag gttccggtac
     8281 caatcctcgt ccggggcctc gcttccgggc gcaacggcga tctgcgcgcg caacgcggca
     8341 actgtcgtaa cggacaagcg cttctgctcg gcatccgtca acgggcgctc ctgttgctgg
     8401 cgggcaatgg tgcgcgtcaa tgccgccagc gccgaccgat cggcccgcat cttcgggtcc
     8461 tgctgcagcg caactgccgc gcggtaaccg cggcggccgt tgtcgaagcg cagctccgcg
     8521 acgtcgagtt tggccggggc gacgcggccc gcatgcaggc ggccgacctg gctggccacg
     8581 ctgatgcggt acggatccag ggcgggcgaa ttcgcgagca ccgcgagcag caccacgatg
     8641 cccgcgagtg cgacattgac ccgcggtagc cagccgagcc atgtgcttcc ccggtgtatg
     8701 gccgccgcgg cataacccac cgcgtgacag gccagcacca ccgccacgag caccgcccag
     8761 aaccgctggt cggtccagcc gtactggccg atgcggagcg ccagcgcgta caacgccagc
     8821 gtggcgtaaa tcggcgacag gaccagcgcg gcgtcgatca accagcgcaa aacccgcgga
     8881 taaggcctgg tgtcgacgcc gtcctgccac acggcattga cgaacgccac cagcagcagc
     8941 aacaacccga gcaacagcgc cgccgccgag cgtgtcgccc acaacggcga aaggcccgtg
     9001 aacggcaggc tggcgagaaa catcagcgca atcagcgcca gcaagggcag caatccgcgg
     9061 cagacagcaa acaggatttg ccgggcgatc cgcagcggat ggtgctgggt gcggccgatc
     9121 aggatgccaa ggccggcgaa caaaccggtg gcaaggaaca cgaacgacgt ggtgtcgaag
     9181 aacaggtcgc ggaagaaccg gatgtgcacc agtgcgaaca gttccgccca cagcctcagt
     9241 accacgaagc acaggcccgt gaacagcagc gctacggcca gggtcaatcc gttctgccac
     9301 gcatggtcga acaggtccgc gtacggcgcg cgccagtggc cctgctcctg ccggcattgc
     9361 aggaaaggca gcgcgacgaa cagcgcgccg cccagcgtga tgccgaacgg cagcagcacc
     9421 gactccgcgt tgatgccggg tgcgccggtg acgctccacg cggcccaact cgccagcagc
     9481 gcgtaaatga tcgcgaccgc ggccgcgtgt tgccagaagc gccggtcgtc caatcgccgc
     9541 accgacaggg tcatcatcgt gggaacggcg aggatcagcg tgtaccagta cacctgcgtt
     9601 ccgcgcaagg agagccagcc ccacaggtgt tcctgtgcgg accaggccgc gtacaacagc
     9661 aacgcctgga ccatcgcgac cagcacgatg aaggcgcggg tctggcgcgg aagcggcaac
     9721 acgtcgtcgc tgcccatggc ggtcctccgg gctggccgcc gcagtatcgc agaaccgcgc
     9781 ctgcaggcga tggcccgcac ggggtaccat cgtccattgg ccgtacaccg gcgccccaac
     9841 cacgaagcct cgggagaaca acatgcaact cggaatgatc ggcctgggca agatgggcaa
     9901 cttcatggcc cagcgcctga tgaagggcgg ccacgacgtg gtcggcttcg atccaagcga
     9961 caaggcgcgc aaggcgctgg tcgatgccgg cggcgaggcg gtcgattcgc tcgcggcgct
    10021 tgtccgtgcg ctcgagccac cgcgcgcggt gtgggtgatg gtcccggccg gcaagatcgt
    10081 ggacgaaacc atcgcgggtc tcgcggccgt gctgggcgaa ggcgacgtgg tgatcgacgg
    10141 cggcaattcc aattacaagg acgaccagcg ccacgcgacg caactcggcg aaggcggcat
    10201 ccatttcgtg gactgcggta ccagcggcgg cgtgtggggg ctcgcggagg gctacagcat
    10261 gatggtcggc ggcgacgccc aggtggtcga tcgcctgcgt ccgatcttcg aggtgctggc
    10321 gcccggcaag gacaagggct ggggccatgt cggccccgtg ggctcgggcc atttcgtgaa
    10381 gatggtccac aacgggatcg aatacggcct gatgcaggcc tacgccgaag gcttctcgat
    10441 cctgaagcac aaacaggagt tcgaactgga tctgcagcag atcgccgaga tctggcgcta
    10501 cggctcggtg gtgcgttcgt ggttgctgga cttgaccgct gacgcgctga agaaggatcc
    10561 ggacatgaag gacatcgcgc cgtacgtctc cgactcgggc gaaggccgct ggaccgtcgc
    10621 cgaagccatc gacctcgacg tgcctgcgcc gatcatcacc gcttcgctga tcgagcgttt
    10681 gcgttcgcgc gatacggatt ccttctcgga caagttgttg tcggcgatgc gcaaccagtt
    10741 cggcggccat gcgatgaaga aggaatgatt tcggtgccac ctgccctgcc cgcgcaggtc
    10801 gtccgctggc acgagttccc cgatgacgtt tcgctgctgg ccgacgcgca atcccggatc
    10861 ctgggcgcgg cgcgcaacgc gatcgcccgc cgccaggcgt tcgacatcgt gctggccggc
    10921 ggcaacaccc cacgcaagct ttaccgcgcg ctgcgccgcg ccgatgccga ctgggcgcgg
    10981 tggcatgtgt ggttcggtga cgaacgctgc gttccggctg acgatcccgg ccgcaacagc
    11041 cggatggcgc gcagcggatg gctcgaccac gtcgcgcttc ccgccgccaa cgtgcacgtg
    11101 atccccgccg agcgcggtgc ccgcgaagcc gccgcgctgt atgcccgcga gttgcgggca
    11161 gccgatggat tcgacctcgt gctgctgggc ctcggcgagg acggccacac cgccagcctg
    11221 ttccccggcc gcgactgggg cgtccacgag gactcgcccg acgccctcgc cgtgttcgac
    11281 gcgcccaagc caccgcccga acgcgtgtcg ctgagtaccc atcgcctgtc caacgccagc
    11341 aacgtattgt tcctggtgga aggggccgga aaacgcgatg ccgtcgcggc atggaaacag
    11401 ggcgaagcga ttcccgcggc gtcgatcgcg ccggaagcgg gcgtggacgt gctgcttgat
    11461 gccgctgcct ccggcacggc gtagccggat ggagccatgc gggttgccgc tgttggcgcc
    11521 gagcagcgaa gcccaacgac gcttgacggc tgttgggctt ctcccggaac aagtccggga
    11581 tcaacccaac ctgccgaagc cgcaaagcag ccgaaccgtg ctctcgtagg ttggcgctga
    11641 gcgcagcgaa gcccaacgag tcaccgcggc cccgccagct cgcccatgtg ggcgcggtag
    11701 taacgcagtt cgtcgatgga atcgcggatg tccgacagcg cggtatgcgc ggaatccttg
    11761 ctgaagccct tgtagatctc gggcgcccag cggcgcgcga gttccttcag cgtggacacg
    11821 tccaggttgc ggtaatggaa gaaccgctcc agcgagggca tctgccggtg caggaagcga
    11881 cggtcctggc aaatcgagtt gccacacatg ggcgacttgc ccggggtcac ccaggcgtcc
    11941 aggaactcca gcgtctcggc ttcggcctgc gcgtggtcga cgccagattc gagcacgcgc
    12001 tgccacagcc cggatttgcc gtgctggttg cggttccagt cgtccatggc gagcagccgc
    12061 gcttcctcgt ggcggatcgc cgtgtgcggc ccctcggcga ggacatgaag gtcgcggtcg
    12121 gtaaccacgg tggcaatttc gatgatcgaa tcgctgtcgg gacagagccc cgtcatttcc
    12181 agatcgatcc agatcaggtt ctcgctcgcg cttccgctca tcgtgtcctc cagccgtgca
    12241 gtctagcaac accgccgggc gcgcacggat tgtcacgcgt acggcgctcc cctacgctgc
    12301 cccgatgcct gccccgaccc tgctgtggta cgactacgaa accaccggca ccgatccggc
    12361 gcgcgatcgt ccggtccagt tcgccgcgat caggaccgac accgatttgc gtccgctggg
    12421 cgatcccgac gtgcggtact gccgccccgc gcccgacgtg ctgccccacc ccgaggcgtg
    12481 cctcgtcacc ggcatcgcgc cgcaggatgc cgaacgcgag gggctcgtcg aagcggagtt
    12541 cgccgccgcg gtgcacgaac ggatggtcga acccggcacc tgctcgaccg gctacaactc
    12601 gatccgcttc gacgacgagg tcaaccgcaa cctgttctac cgcaacttct tcgatcccta
    12661 cgagcacgtg tggaagaacg gcaactcgcg ctgggacatc atggacctcg cgcgcgcctg
    12721 ctacgccctg cgcccggcgg ggatcgaatg gccggtgcgc gaggacggcg cgccatcgtt
    12781 caagctggag cacctggcgg ccgccaacca cctcgggcag cggcgcgcac acgatgcgct
    12841 gtcggacgtc gaggcgacca tcgcgctggc gcgtttgctg cgcgaccgcc agccgcgctt
    12901 gtacgactgg tgcttcgccc tgcgcgacaa gaataccgcg tcgccgaaac tggcggcggc
    12961 cctgcccacg ctgcagccgc tgctgcacgt atcaggccgc tacgcgccgg cacgcggctg
    13021 cctcgccatg gtcgtacccc tcgccgaaca cccgacgcgc gccggcacgt tcatcgtcgc
    13081 cgacctcgat gtcgatcccg acggctggat cgacctcgat cccacggatc ttgccgagcg
    13141 gatgttcacc cggcgggtcg atctgcccga ggacatcgat cggccgccgc tcaaggaaat
    13201 acacgccaac cgatcgccct tcctggcgcc gatcgagacg ctgcgcaacg tggacgtcgt
    13261 ccgcatctgc ctcgatccgc cacgctgcct gcggcacctc gagcggattc gcgcgagtgt
    13321 cgggctggcc gaaagggtgc gcagcgcctt cgccggccgg gtcgcggcgt ggacggaccg
    13381 caacgatccc gagctggcga tgtattccgg cttcccctcg ccggcggaca aggcccgttg
    13441 cgaccgggtg cgcgcaacgc cgcccgcgca gctgcactcg acagacttcg ggttcggcga
    13501 cccgcgctat cccgaactgc tgttccgcta ccgcgcccgc aactggcccg aaaccctgga
    13561 tgcgggcgag cgcgagcgct ggtccgcatt cgtgcgcgac aaattgacct gcgacaacgg
    13621 cctcggccgc ctgacgctgc aacaatattt cgacgtcatt gcgcgcctgc gcgccgaagt
    13681 gccgcccggc ccgaagcagg ccctgctgga ccggctgcag gactggggcg aaaccgtgcg
    13741 gcaggaattc gagcttcgcg aaggcaccgg ccaactggtg tgaagctcgt tgccagccgc
    13801 cgttgatggg gaatccgttc ggaccatgcc gcaagcctac ttctcgcccg caaccttccg
    13861 gttcctgcgc gcgctcgatc gcaacaacaa ccgcacatgg ttcaaggcgc accagccggg
    13921 ctacgagcgc gaggtccgcg agccgttcct gcaactgatc gccgatttgc aggtaccggt
    13981 cgcgaagatc agcacgcact accgtgccga tccgcgcaag atcgggggtt cgctgttccg
    14041 cgtgcagcgc gacacccgtt tcagccacga caaggcgccg tacaagccct gggccggcgc
    14101 acggctgttc cacgagcgtc gccgtgagat cgccgcgcct tcgttctacc tgcacatcgc
    14161 ccccggcgac tgcttcatcg gcgcaggcct gtggcacccg gaaaaccaca cattgcgcaa
    14221 tgtccgcaac ttcattgcag acaacccggc ggcatggcaa cgcgccgccc acggccgcgc
    14281 cttccggagc cggtacgcgt tccggggcga atcgctgata cgaccgccgc acggctttcc
    14341 tccggaccat ccgctgatcg aggacttgaa gcgcaagagc ttcgccgtcg gcaccggctt
    14401 cgacgacacg ctggcctgct cgccgaagct gctcccgttc gtatcggatc atttcaggcg
    14461 gctggcgccg ctggtcgact acctctgcgc cgcgcagaac ctggagttct gacccatgcg
    14521 acatgccatg cgcgcaaccg tccttgccgt cctgctcgcc tgcttgtgcg ggacggcggg
    14581 tgccgccgga cagaggccgc tgcaaatcct gttcgtcggc aacagcctca cctatgtcgg
    14641 caacctgccg gcggtcctgc aagcgctcgc tgccgacaac ggcaagtcac tgcaagccga
    14701 catgctggtc aagggcggcg cgacgctgac gcagtggctg gacggcggcg cggtggactc
    14761 tgccctgggc gccaggcact acgacaaggt ggtgcttcag gaacgcggct atgacttcgc
    14821 ctgcggcttc ggaccgcaag tttgcgagga tgcgcggcag gcgctgcgcg ctcgcagaca
    14881 ttgcacggaa ggcaggcgcg cagccgatct tgctgggaac ctaccaggtg gcgccggagg
    14941 cctcgcaggc aatcgtcgcg gccgaatcca ggtcggccca aagggagaac atgccgtaca
    15001 tcgcggtgtc gcagcgcctg aacgaggggc gcgcgcagtt ccagcatgcg gactggttcg
    15061 ccaaggccgg gcatcccggc cacgacctgg tcctcctgga agcggttctg ctttacgagc
    15121 aactgtacgg caccgcacca ccggcgaagg cactggaggt gcgcgcaccg atgttcgttc
    15181 ctggcagcaa gttcgcacca ccggcgccgg tatccctgcc attgctgccc gaggtggcgc
    15241 tggccggcgg ctacgattat tcgagcacgg agatggcgaa cgcgatcgaa ctggcgggta
    15301 gccactgacg cgccaaaatg tggagcgcgt cttttcgcgt gcggaaggag gagcaagcgc
    15361 ttcctaaatt gatgtcgcag cgcagtcgtt gcaagagcgc gcgatgcccg cgatgggcgc
    15421 taccgcaacg tcgatcgcga ccatggggcg ctcctaagcc ggcgaccggc cgcgtgcttg
    15481 cgccgcggtt cacgcctccg cgaatggaaa cgcgatcaca tcccgaatgt cgtcggtgcc
    15541 gagcaattgc atcagcagcc ggtcgacacc gagtgcgacg cctgcgcagt cgggcagatg
    15601 gtcgagtacc gccagcaggt tttcgtccag cggaagctcg tcgaggccgt gttcgcggcg
    15661 acgcaggttt tcgtgctcga agcgcgcgcg ctgctccgcg gcgtcgttca actcgtggta
    15721 gccattcgcg agttcgtgct gcccgaggta gatttcgaag cgctccgcca ccggcgggtt
    15781 gccggcaatg cgggcaagcg agcattgcga ggcgggaaag tcgtagacga tggtgatgcc
    15841 gccgcccggc agcgctggct gcaggcggtg cgtcaccagc aggtccagcc agtcgtcgcg
    15901 gccgaggccc gaggattcga tgcggaattc cgcgagcggc gcgcgcagcg cgccctcccc
    15961 cgcctcgaac ggatcgagcc gcaggtggtc gcggaaccag tcgcgatagc tcgtcgtcac
    16021 caccggcgcg tcgcgctgga ccagcgccag cgccacacgc accagttgcg cggtttcgag
    16081 cgcgagccgc cggtgctcga agccgacccg gtaccactca agcatcgtga attccggatt
    16141 gtgcgaggaa cccgcctcgc cgttgcggaa cacgcgaccg agttcatagc aatcgccgaa
    16201 gcccgacgcg agcaggcgtt tcatcgggta ttcggccgag gtccgcaggt agcgcgtgcg
    16261 cggaccggcc gcgatgggtc cggcaaaccc ggtgacgaac cccgcgatgt tgggttcggt
    16321 gttgccggcc gccgagagga tcggcgtttc gacttccgcg acgccgcgat gcgcgaagaa
    16381 atcgcggatc agcgcgttga gccgcgaccg caggcggatc gcctcgcact tcgcggcgag
    16441 cgccgtcatg tggtccaggc agcgttctgc aggatcaccc tgccgccttc gcaatcggcg
    16501 taggtgcggc cgacccgctc ccaatagggg ttggcattcg gcactcga
//
LOCUS       DASSNH010000104         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1644167, whole genome shotgun sequence.
ACCESSION   DASSNH010000104 DASSNH010000000
VERSION     DASSNH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644167"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="VFK00_03725"
     CDS             <1..1849
                     /locus_tag="VFK00_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7592096.1"
                     /translation="LGQMLTHRFIDKAEYDQAIAAPDDAAPHEPPVEVDAPYLAEMVR
                     QQALQKLGNQAQTDGYVIRTTLDSRMQSLANHALRSKLEEYDHRHGYRGPEAHVALPQ
                     PATPASLQKALAGYNDVSGLYPAIVTEVGDDDAKVYVQGGATIKLSVADMKWAMGFAR
                     KAKTGGKGAASILRPGDIIRVREVADADASAGAPAKPAKDGKAAIPDKKHWELQQIPA
                     AQSAMVVLSPDDGAVKALVGGFNFQLSKFNRAVQTQRQPGSSFKPFIYSAAFSRGYTP
                     SSIINDAPIALPDPSKPGGLWTPQNDDNKFRGPMRLREALAQSVNLVAIRLLDAVGVR
                     YVREYATRFGFPLDAIPANLSMALGTASVSPMAMARAYAVFANGGFLVNPYYIAEIDD
                     RTGKAIYKADPLQACRTCEARLLDEGKTTAAPAQPAAAAGDPGAMADTANALSQGESN
                     KPLLAPRVVDPRNDYLMTSLLQSVIYGGGTGAAAAKQLGRDDLAGKTGSTNDHHDGWF
                     CGFNADLVATVWVGFDDYRSLGHGEFGARTALPIWMAFMGPALQGTPEARLPMPPGIV
                     TATIDRTTGLPAPPGDGNTMTERFKIEDLDRLRTEAKNQQNQAPAYDIF"
     gene            2163..2801
                     /locus_tag="VFK00_03730"
     CDS             2163..2801
                     /locus_tag="VFK00_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592097.1"
                     /translation="MHKTAGYHRIHAEGRGHQLRRRIALEAARLISEHGIRDFRMAKR
                     KAAQHIGVSDEGFLPRNREIEDALREHQRLFRAEEQPHALRTRREAARQAMRFLDRFE
                     PRLVGAVLEGTADTHSPVSLHVFNDSPETVSGFLHDHGIAFETRARTLRIDRVRNAEF
                     PVLLFSADDVPMDVTVFPRDALRQAPLDPVSERPQQRASLAQVEALLMETMA"
     gene            complement(3029..>3340)
                     /locus_tag="VFK00_03735"
     CDS             complement(3029..>3340)
                     /locus_tag="VFK00_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET7592098.1"
                     /translation="VLNELGVDDPLLDVALKLEEAALKDDYFVERKLYPNVDFYSGII
                     YKALRIPTEMFTVMFAIARTAGWIAHWIEQHEMPGGRIGRPRQVYVGATPRDYVAPDK
                     R"
BASE COUNT          613 a         1154 c         1038 g          535 t
ORIGIN      
        1 gctggggcag atgctgacgc accgtttcat cgacaaggcc gaatacgacc aggcgatcgc
       61 cgcgcccgac gacgccgcac cacacgaacc gccggtcgag gtcgacgctc cgtatctcgc
      121 cgaaatggtg cgccagcagg cgctgcagaa gctcggcaac caggcgcaga ccgacggcta
      181 cgtgatccgc accaccctcg acagccggat gcagtcgctg gcgaaccacg cactgcgcag
      241 caagctcgag gaatacgacc accgccacgg ctatcgcggt ccggaggcgc acgtcgcact
      301 gccgcaaccg gccacgcctg cctcgctgca gaaggcgctg gccggctaca acgacgtcag
      361 cggtctgtat cccgccatcg tcaccgaggt gggcgacgac gatgcgaagg tgtacgtgca
      421 gggcggggca accatcaagc tgtccgtcgc cgacatgaaa tgggccatgg gttttgcacg
      481 caaggccaag accggcggca agggcgcagc ctcgatcctc cggccgggcg acatcatccg
      541 cgtgcgcgaa gtggcggatg cggacgcgtc cgccggcgcg cccgccaaac ccgcgaagga
      601 tggcaaggcc gccatccccg acaagaagca ctgggaactc cagcagattc ccgccgcgca
      661 gtccgcgatg gtggtgctgt cgccggacga cggcgcggtg aaggcactgg tcggcggctt
      721 caatttccag ttgagcaagt tcaaccgcgc ggtgcagaca cagcgccagc cgggctccag
      781 cttcaagccc ttcatctatt cggcggcttt cagccgcggc tacacgccgt cttcgatcat
      841 caacgatgcg ccgatcgcgc tgcccgatcc ctccaagccg ggcgggctgt ggacgccgca
      901 gaacgacgac aacaagttcc gcggaccgat gcgcctgcgc gaggcgctgg cgcaatcggt
      961 gaacctggtg gcgatccgcc tgctggacgc agtcggcgtg cgctacgtgc gcgagtatgc
     1021 gacgcgattc ggtttcccgc tggacgcgat tcctgccaac ctctcgatgg cgctcggcac
     1081 cgcgtccgta tcgcccatgg cgatggcgcg tgcctacgcc gtgttcgcca acggcggttt
     1141 cctggtcaat ccctactaca tcgccgaaat cgacgaccgc accggcaagg ccatctacaa
     1201 ggccgatccg ctgcaggcct gtcgcacctg cgaagcgcgc ctgcttgatg aaggcaagac
     1261 cacggcggcg ccggcacaac ccgcggcggc ggcaggcgat ccgggcgcga tggccgacac
     1321 cgccaacgcg ttgagccagg gcgagtcgaa caaaccgttg ctcgcaccgc gggtcgtcga
     1381 tccccgcaac gattatctga tgacctcgtt gttgcagagc gtgatctacg gcggcggcac
     1441 cggtgccgcg gcggccaaac aactcggccg cgatgacctt gccggcaaga ccggttccac
     1501 caacgaccac cacgatggct ggttctgcgg cttcaacgcc gacctcgtgg ccacggtctg
     1561 ggtgggattc gacgactatc gttcgctcgg gcacggcgag ttcggtgcgc gcaccgccct
     1621 gccgatctgg atggcgttca tgggcccggc gctgcagggc acgcccgaag cgcggctgcc
     1681 gatgccgcct ggcatcgtca ctgcgaccat cgatcgcacc accggcctgc ccgcgccccc
     1741 gggcgacggc aacaccatga ctgagaggtt caagatcgag gatctcgacc ggctgcgcac
     1801 cgaggcgaag aaccagcaga accaggcgcc tgcgtatgac attttttagc gatcgtccat
     1861 gatcgctgcc tcgtcggagc gactacgcaa cgtggtcact ccaagccatg gaagaccaga
     1921 gcggccatcc atggcctgac acactcatgc gatcgtccat gatcgctgcg ccactctgcg
     1981 acatcgcaac ctggacgttt caaagccgga atcttccaga acggccctcc atggcctgac
     2041 ttctcaccaa aaaccgcctt gttcgcagtt cccttcgcga ttgttttcgc gcgccgcacc
     2101 ccctttgctt tttgtggccg ctttttgcga cactggaccc aacgcggcta cagggtgttt
     2161 ccatgcacaa gaccgccggg taccaccgta tccatgccga gggccgcggc catcaattgc
     2221 gccgccgcat cgccctcgaa gccgcacgcc tgatcagcga acacggcatc cgcgatttcc
     2281 ggatggccaa acgcaaggcc gcacagcaca tcggcgtcag cgacgagggt ttcctgccgc
     2341 gcaatcggga gatcgaggac gcattgcgcg aacatcagcg gctgttccgc gccgaggagc
     2401 aaccgcacgc gctgcgaacc cgtcgcgaag ccgcacggca ggccatgcgt ttcctggatc
     2461 ggttcgaacc ccggctggtc ggcgcggtgc tggaaggcac cgccgacacc cattcgcctg
     2521 tgagcctgca tgtattcaac gactcgcccg aaaccgtcag cggtttcctg cacgaccacg
     2581 gcatcgcgtt cgagacgcgc gcacgcacgc tgcggatcga ccgcgtgcgc aatgcagaat
     2641 tccccgtgct gctgttcagc gccgacgacg tgcctatgga cgtgaccgtg tttccgcgcg
     2701 acgcgttgcg gcaagcgccg ctcgatcccg tcagcgaacg tccgcagcaa cgtgcgtcac
     2761 tggcccaggt cgaagccttg ctgatggaga cgatggcgta ggacgggagc agccgcgcag
     2821 cggcgtatcc cgcggctggg ataggcgaat ggcgggatgc gctgcgctcc tcccgcccta
     2881 cgcgctggct gtagagcggt aaaggctaaa gttggcggct gtgacgcctg cgaagggcaa
     2941 agagcaaaag cggattcgca gttcctcttc accctttgca gcgccgaagg cgcgactttg
     3001 cagctcgcgg agcgagcggc ttcccgcgtc agcgcttgtc cggcgccacg taatcgcgtg
     3061 gcgtggcacc gacgtacacc tgccgcggcc ggccgatgcg gccgccgggc atttcgtgct
     3121 gttcgatcca gtgcgcgatc cagccggcgg tccgcgcgat cgcgaacatc accgtgaaca
     3181 tctcggtcgg aatccgcaac gccttgtaga tgatgcccga gtagaaatcg acgttcgggt
     3241 acagcttgcg ctcgacgaag tagtcgtcct tcaacgccgc ctcttccagc ttcaaggcca
     3301 catccagcaa cggatcgtcc acgcccagtt cgttcagcac
//
LOCUS       DASSNH010000105         5603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1200508, whole genome shotgun sequence.
ACCESSION   DASSNH010000105 DASSNH010000000
VERSION     DASSNH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200508"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VFK00_03740"
     CDS             complement(<1..984)
                     /locus_tag="VFK00_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET7592099.1"
                     /translation="MSREDYRRKRDLAKSGEPDFDSPSAAEARAIFVVQLHHASRRHF
                     DFRLQVGDALKSWAVPKGPSFDPGVKRLAVEVEDHPVAYAGFEGDIEEGYGKGHVDLF
                     DRGVWSSDGDPEQQLEKGHLHFELFGERLKGSWHLVRSHRKERQPAWFLIKGKDRYAG
                     KREADDLLDEKMVESTRRAARTTGERALARKVARKSAARRRTRGAPAGKPAPSLKPAG
                     LNHARKAAADPGFFKPELARLRDKAPEGDKWLHEVKWDGYRILTAIADGEVNLWSRNA
                     LPWNERAPEIVDAIGDLGLKSARLDGELIALDNGLSDFNLLQKTLSGEASAP"
     gene            complement(1001..1186)
                     /locus_tag="VFK00_03745"
     CDS             complement(1001..1186)
                     /locus_tag="VFK00_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008577726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592100.1"
                     /translation="MTKLDKPIRREIEVDGKAYTLTIDPLGMKLVEKGHRNGVALTWK
                     NMLSGDAALAAALQASV"
     gene            1308..1727
                     /locus_tag="VFK00_03750"
     CDS             1308..1727
                     /locus_tag="VFK00_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592101.1"
                     /translation="MRELDDFADLREPRDFDLPEEDLRVEDFFADDLDADFRVEDFFE
                     VPEDFLADDFFAEDFRAADFLAEDLFDGDFFDEDLFDEDFFDADLPDEDEFDFEDFFA
                     PLEPVRPLPDFLPPPDCLFTVAQARRSASLSETPRSS"
     gene            complement(1861..2331)
                     /locus_tag="VFK00_03755"
     CDS             complement(1861..2331)
                     /locus_tag="VFK00_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7592102.1"
                     /translation="MDAIKLLKAGHYQIRDLLEDLCDADMEARQVRSDLLAQAVEALD
                     VHSRIENEILYPAFAGVAEREGDVRLVQDAAEENHVIADLLLPDLLETDISSEFFGGR
                     AKVLCQLVDHHIRQEEADLFPRIKKLMPGPERNALGERLLQRKQELRDGVPSRA"
     gene            2501..3394
                     /locus_tag="VFK00_03760"
     CDS             2501..3394
                     /locus_tag="VFK00_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7592103.1"
                     /translation="MPRPIWTGTISFGLLNIPIRLMTAERRVDLHFRMLDNRDQSPVR
                     YERVNAETGEEVPWKDVVKAFEYDKGNYVVMEKEDFKKAAPDSAESVDIEAFIDPTEI
                     GPEYYEKPYYLVPAKKAEKGYVLLRETLNRSGLAGLARVVIRTREYLSLVLPRGDALV
                     LILVRFPQELVPADEYDFPQADPGSYRINDKELDMAKSLVESMVSKWDPKEYRDEFRS
                     RLQKVIEARVRSKGGKVKVEEEEAPAADTATNVVDFMSLLKKSIDSNKRTPAKTKSTT
                     TKKAAAKKRGGSSKGKKKAAK"
     gene            3623..4270
                     /locus_tag="VFK00_03765"
     CDS             3623..4270
                     /locus_tag="VFK00_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7592104.1"
                     /translation="MAQLFPRHFGFYFLLGIAVAVGIGLAWVYLWRAQMAYPAGLNDP
                     VEQPVPFSHKHHVGDDGIDCRYCHQSVEQSAFAGIPPLSTCMTCHSQLYTHQPVLKPL
                     LAAYRSGVPLHWKRLYKLPDFVYFDHGIHIAKGVGCASCHGEVDQMPLTARVRSLTMQ
                     WCLRCHRDPARYVRPRDEVFNLHWHAQDQLALGAELVRKYHIDTRRMTDCSLCHR"
     gene            4258..>5603
                     /locus_tag="VFK00_03770"
     CDS             4258..>5603
                     /locus_tag="VFK00_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HET7592105.1"
                     /translation="MSPVKPTPSRPQAAFDPDAAREQLAAKRGPVYWRSLEELADSEG
                     FHRWLRQQNPRLGEALLMDRRGFLKLLGASLALAGLAACSHPPQTEIVPYVHKPVGQV
                     DGLPRYFATVLTREGHAQGVLVADHQGRPTKAEGNPQHPASLGGTDIFAQAAILQLWD
                     PDRSQSIRHRGITSTWDDFIAKALAMSARFEDNGGDGLHVLTGPVTSPTLTAQLDALG
                     QRFPKSRWHVHRPGGRNAYDGTRLAFGQPLSTRLHMDRARVVVALDADPLSDPAAGTR
                     YGHDYAITRSPEQNDGAMSRLYAVEPTPSLTGLMADHRLPLGFREVGEFARQLAGKIG
                     VTMPPGAAPAAGEQTRWLDAIAADLQAAHGAALILVGAAQPPWLHALGHAMNAALGNV
                     GRTIDYSAPVETLPGEDDGLAGLVSAMRAGRVDTLLALEVNPVYDAPAELEFADAL"
BASE COUNT          931 a         1818 c         1841 g         1013 t
ORIGIN      
        1 cggcgcgctg gcctcgccgg acagcgtctt ctgcaacagg ttgaagtcac tcaagccgtt
       61 gtccagcgcg atcagttcgc cgtccagccg ggccgacttc aatccgaggt cgccgatcgc
      121 gtccacgatt tccggcgccc gctcgttcca ggggagcgcg ttgcgcgacc acaggttgac
      181 ctcgccgtcg gcgatggcgg tgaggatccg gtatccgtcc cacttcacct cgtgcagcca
      241 cttgtcgcct tcgggcgcct tgtcgcgcag ccgggcgagt tccggcttga agaagcccgg
      301 gtccgcggcc gccttgcgcg cgtggttgag ccccgccggt ttcagcgaag gcgcgggttt
      361 gcccgcaggg gcgccgcggg tccgcctgcg cgcggcgctc ttccttgcaa ccttgcgcgc
      421 cagtgcacgc tctccggtgg ttcgcgcggc gcggcgagtg ctctcgacca tcttctcgtc
      481 cagcaggtcg tcggcttcgc gcttgccggc gtagcggtcc ttgcccttga tcaggaacca
      541 ggcgggctgg cgctccttgc ggtggctccg caccaggtgc caggaaccct tgagtctttc
      601 gccgaacagt tcgaagtgca ggtggccctt ttcgagctgt tgctccggat cgccgtcact
      661 cgaccagacg ccgcggtcga aaagatccac gtggcccttg ccatagcctt cctcgatgtc
      721 gccctcgaac ccggcatagg cgaccggatg gtcctccact tccaccgcga ggcgcttgac
      781 cccgggatcg aaactcgggc ccttgggaac ggcccagctc ttcaacgcat cgcccacctg
      841 caggcggaag tcgaagtgcc gccgggacgc atggtgcaac tgcaccacga agatcgcgcg
      901 cgcctcggcg gcggacgggc tgtcgaagtc cggttcgccg gacttcgcga ggtcgcgctt
      961 gcgccgatag tcctcccggc tcatcgctgg ccgggcaaca tcagaccgac gcctggagtg
     1021 ccgccgcgag tgccgcgtcg ccgctcaaca tgttcttcca ggtgagcgcg actccgttcc
     1081 tgtgtccttt ttccaccagc ttcatgccga gtggatcgat cgtcagcgtg taggccttgc
     1141 cgtcgacctc gatttcgcgc cggatgggct tgtcgagctt ggtcatcgtc gttgctccat
     1201 gtcgtgtccg gcaggcggac gccggtgggt tcctacggta tgcggatccg cggggcgggt
     1261 tcgccaccgc gggtccatgg ctggatcatc gccgggacga accggacttg cgcgagctgg
     1321 acgactttgc ggacttgcgc gagccgcggg atttcgacct gcccgaggag gacttgcgcg
     1381 tcgaggattt cttcgcagat gacctggatg ccgatttccg cgtcgaggat ttcttcgagg
     1441 tgcccgagga cttccttgcc gacgatttct tcgcggaaga cttccgtgct gcagatttcc
     1501 ttgcggaaga cctcttcgat ggcgatttct tcgacgagga ccttttcgac gaggactttt
     1561 tcgacgcgga cttgccggac gaagatgagt tcgacttcga ggacttcttc gcgccactgg
     1621 agcccgtgcg tccgctaccc gacttcttgc caccgccgga ttgcttgttc acggtagccc
     1681 aggcgcgccg ttccgcttcc ctttccgaga cgccacggtc ctcgtagccc tcctcgatgt
     1741 gctcggcctt ccgcttctgc ttgtcggtat agctggactt gtctccacgt ggcatgactg
     1801 cgtctccttg cgtgcgttgt gggagtgggt gtgccctgcg tcatggtcgc gggtcgcggc
     1861 tcacgcccgg gacggaaccc catcccggag ttcctgtttg cgttgcagga gccgttcgcc
     1921 gagcgcgttg cgctccgggc cgggcatcaa cttcttgatg cgcgggaaca gatcggcttc
     1981 ctcctgccga atgtggtggt cgaccagttg gcacaagacc ttggcacgac cgccgaaaaa
     2041 ctcggatgaa atgtccgtct cgaggaggtc cggcaacagg aggtctgcga tgacgtggtt
     2101 ctcctcggcg gcgtcctgca cgagtctgac gtccccctcg cgctcggcaa ccccggcgaa
     2161 tgccgggtag agaatttcat tctcgatgcg cgaatggaca tccaacgctt cgacggcctg
     2221 cgcgaggagg tccgaacgca cctgcctggc ctccatgtcg gcgtcgcaga gatcttctag
     2281 caagtccctg atctggtagt ggccagcctt cagcaacttg atggcgtcca tgacgtcagc
     2341 attccatgcc ctccagccca gcctaggcgc aaatggatga atgcggagtg tatggcaagg
     2401 gtccggatgt gccacgcgcc gcgcactgac acgggcttca cccggcgcgg cgcaaactgc
     2461 tcgtccggcc ggaaacaggc gcccctcggg ggatacgaac atgccacgcc ccatctggac
     2521 aggaacgatt tccttcgggc ttctcaacat cccgatccgg ctgatgacag ccgagcggcg
     2581 ggtggatctc catttccgga tgctcgacaa ccgcgaccag tcgccggtcc gatacgagcg
     2641 cgtgaatgcg gaaaccggcg aagaggtgcc ctggaaggac gtcgtcaagg ccttcgagta
     2701 cgacaagggc aattacgtcg tgatggaaaa ggaggacttc aagaaggcgg cgcccgattc
     2761 ggccgagtcg gtcgacatcg aagccttcat cgatccgacg gagatcggcc ccgaatacta
     2821 cgaaaagccc tattatctcg tgccggcgaa gaaggccgag aagggctatg tgctactcag
     2881 ggaaaccctg aaccgctccg ggctcgcggg cctggcgcgg gtggtgatcc gcacccgcga
     2941 atacctgtcg ctggtgttgc cgcggggcga cgcactggtg ttgatcctgg tgcggtttcc
     3001 gcaggagctc gtgccggccg acgaatacga ctttccgcag gcggatcccg gttcctaccg
     3061 catcaacgac aaggaactcg acatggcgaa gtcgctggtc gaatcgatgg tgtcgaaatg
     3121 ggacccgaag gaataccgcg acgagttccg gtcacgcttg cagaaggtca tcgaggcgcg
     3181 ggtgcggtcg aagggcggca aggtgaaggt cgaggaagag gaggcgcccg cagccgacac
     3241 cgcgaccaac gtcgtcgact tcatgtcgct gctgaagaag agcatcgact cgaacaagcg
     3301 gacccccgcc aagaccaagt ccacgacgac gaagaaggcg gcagccaaga agcgcggcgg
     3361 cagctcgaaa ggcaagaaga aggcggcgaa atgaagagtg ggcgaagtcg gcgcagcgca
     3421 tcgaggcttt cccggcaccg cggtcgcgca ttcctcattg ggtaaacggg gcatcgtgcg
     3481 cgccttcgcg tgaaagacgc gcgaacgctt tgcgcaaacg tctgcaccgc gtgaaatcgg
     3541 cggatttccg cttgcggcat gacgatccgg tgcgttagct tgccttgcgt ggcacgaggg
     3601 acgctcgatc aagggatgat cgatggcgca gttgtttccg agacatttcg gcttttattt
     3661 cctgctcggc atcgccgtcg cggtcggcat cggactagcc tgggtttacc tgtggcgcgc
     3721 gcagatggcg tatcccgcag ggctcaatga cccggtggag cagccggtcc ccttcagcca
     3781 caaacaccat gtcggcgacg acggcatcga ttgccgctat tgccaccagt ctgtcgagca
     3841 gtccgcgttc gcgggcattc cgccgctctc gacctgcatg acctgccact cgcagttgta
     3901 cacgcaccag cccgtgctga agccgctgct ggccgcatat cgcagcggcg tgccgttgca
     3961 ctggaaacgc ctctacaagc tgcccgactt cgtctacttc gaccacggca tccacatcgc
     4021 caagggagtc ggttgcgcga gttgccacgg cgaggtcgac cagatgccgc tgacggcacg
     4081 ggtgaggagc ctgacgatgc aatggtgcct gcgctgccac cgcgatcccg cgcgatacgt
     4141 tcgcccgcgc gacgaggtgt tcaacctgca ctggcatgcg caggaccagc tcgcccttgg
     4201 cgcggagctg gtgcggaagt accacatcga tacgaggcgc atgacggact gttcgctatg
     4261 tcaccggtga agccgacgcc atcccggccg caggccgcgt tcgatccgga cgcggcgcgc
     4321 gagcagctcg ccgcgaagcg cggaccggtg tactggcgca gcctggagga gctcgccgac
     4381 agcgagggtt tccatcgctg gttgcgccag cagaatccca ggctcggcga ggcgctgctg
     4441 atggaccggc gcggtttcct gaagctcctg ggtgcctcgc tggcgctcgc ggggctcgcg
     4501 gcctgcagcc acccgccgca aaccgagatc gtgccgtacg tccacaagcc ggtgggccag
     4561 gtcgatgggc tgccgcggta tttcgcgacc gtgctgacgc gcgaaggcca tgcgcagggg
     4621 gtgctggtcg cggatcacca ggggcgaccg accaaggccg agggcaatcc gcagcatccc
     4681 gccagcctcg gcggcaccga catcttcgcg caggcggcga tcctgcaact gtgggatccg
     4741 gatcgttcgc aatcgatccg gcatcgcggg atcacttcca cgtgggacga tttcatcgcg
     4801 aaggcactcg cgatgtccgc gcgcttcgag gacaacggcg gagatggatt gcacgtcctg
     4861 accggaccgg tcacgtcgcc gacgctgacc gcgcaactcg atgcgctcgg acagcggttt
     4921 ccgaagtcgc gctggcacgt gcatcggccg ggcgggcgca acgcgtacga cggcacacgg
     4981 ctcgcgttcg gacaaccctt gtcgacgcgg ctgcacatgg accgggcgcg ggtggtggtg
     5041 gcgctggatg ccgaccctct gtcggatccc gcggccggta cgcgctacgg gcacgactac
     5101 gccatcaccc gctcgcccga acagaacgac ggcgcgatgt cgcgcctgta cgcggtcgag
     5161 ccgacgcctt ccctgaccgg gttgatggcc gaccatcggc tgccgctggg atttcgggag
     5221 gtcggcgaat tcgcgcggca actcgccgga aagatcggtg tcacgatgcc ccccggtgcc
     5281 gcgcccgcag caggagaaca gacgcgctgg ctcgatgcaa tcgccgcgga cctgcaagcc
     5341 gcccacggcg cggcgctgat cctcgtgggc gctgcgcaac caccgtggct gcacgcgctg
     5401 ggtcacgcga tgaacgcggc gctcggcaac gtgggaagga ccatcgacta cagcgcgccg
     5461 gtcgaaacgc ttcccgggga agacgacggc ctcgccggtc tcgtgtcggc aatgcgtgcg
     5521 ggcagggtcg acaccttgct ggcactggag gtcaatccgg tgtacgacgc gccggcggag
     5581 ctcgaatttg cggacgcgtt gaa
//
LOCUS       DASSNH010000106         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1153239, whole genome shotgun sequence.
ACCESSION   DASSNH010000106 DASSNH010000000
VERSION     DASSNH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153239"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..713
                     /locus_tag="VFK00_03775"
     CDS             204..713
                     /locus_tag="VFK00_03775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7592106.1"
                     /translation="MTLNATMQTSRGPIHLRLFEDKAPVTVASFVNLVQRGFYDGLSF
                     HRVIPDFMVQGGCPEGSGRGGPGYRFEDETRADLKHDKPGVLSMANAGPGTNGSQFFI
                     THVATPWLDGKHTVFGGVVSADDQKVVDAIRGGDTIEKVTIEGDASSLLDAQASRVAE
                     WNKVLDARR"
     gene            864..2102
                     /locus_tag="VFK00_03780"
     CDS             864..2102
                     /locus_tag="VFK00_03780"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7592107.1"
                     /translation="MLTLASRMSRAKPSAIMVIADKAKQLRAAGRDIVNFSIGVPNFL
                     PGEHVYDAVREALKHDTGAYGSNRGREDLLDAFLAHMKATGLDGYKRSNIAVGIGAKQ
                     VLYNLCEALLDEGDEIVFPTPYWTSYVDIAEILGARINLLPCPPEQNYKLTPAQLDRA
                     LADKPKVFLFNNPSNPTGMVYTREEIAALADVMAKHPDTWIIADDIYNRMVFDGVGYH
                     NVVQFRPELRERTAIVDSLSKTYGMPGWRVGFVAAPEPLANAVVVLNSNHITNIPEIV
                     TAAAVAALTGPQDITKEKCAEFARRRDEVLGALLSIPGVKCPRPQGAFYAFPDISVAF
                     GKSHNGTLIDNDVDFCAALLDAKGVATVPGSAFGEPRALRISYTCPRAQLESGLQRIK
                     EFFAELVEVAPVARVGAAGA"
     gene            2208..>2474
                     /locus_tag="VFK00_03785"
     CDS             2208..>2474
                     /locus_tag="VFK00_03785"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7592108.1"
                     /translation="MKAPVRIAVTGAAGQIGYALLFRIAAGDMLGKDQPVILHLLEIT
                     PALPALQGVVMELDDCAFPLLQGIVATDDANVAFKDVDYALLVGA"
BASE COUNT          454 a          821 c          800 g          399 t
ORIGIN      
        1 aagcggccgt accttcaaga ggaaagatga tcggcgggcc gatcaggccg ccgcgactgg
       61 acacattata accgggcgcg cgctccggcg cttgcgatgc tgaacaggct tgttaagcta
      121 gccggcttcc ccacggaaat gctgaagaag ctgctgcgct gggcagctgg cgcttcggag
      181 tttccccaac ccaaggagcg gccatgaccc tgaatgccac catgcagacc tcgcgcggtc
      241 ccattcatct gcggctgttc gaggacaagg cgccggtcac ggtcgccagc ttcgtgaacc
      301 tcgtccagcg cggattctac gatgggctga gcttccatcg cgtgatcccg gatttcatgg
      361 tccagggcgg ctgccccgaa ggctcgggtc gcggcggccc gggttaccgg ttcgaggatg
      421 aaacgcgtgc ggacctgaaa cacgacaagc cgggcgtgct ctcgatggcc aatgccgggc
      481 ccggcaccaa cggcagccag ttcttcatca cccacgtggc gacgccgtgg ctggatggca
      541 agcacacggt gttcggcgga gtggtttcag cggacgacca gaaagtggtg gatgcgatcc
      601 gcggcggcga cacgatcgag aaggtcacga tcgaaggcga tgcctcgtcg ctgctcgacg
      661 cgcaggcttc gcgcgttgcc gaatggaaca aggtgctgga cgcgcgccgc tgacgcgcgt
      721 cggaaaacgg ggcaaggtcg cgcgcgcgat ccgcgcgggc gccgcatgat agaattgtac
      781 gttgtcaatc cgtccgcgat tggctgatgc aagcccgcca tgccgagcac ccgctccgca
      841 atccgtcaga aacgaggaat gtcatgctga cactcgcctc ccgcatgagc cgcgccaagc
      901 ccagcgcgat catggtgatc gccgacaagg ccaagcagtt gagagccgcc gggcgcgaca
      961 tcgtgaactt ctcgatcggc gtgccgaatt tcctgcccgg cgagcacgtg tacgacgccg
     1021 tgcgcgaggc gctgaagcac gataccggcg cctacggttc caaccgcggc cgcgaagacc
     1081 tgctcgacgc gttcctggcg cacatgaagg cgaccggcct ggacggctac aagcgttcca
     1141 acatcgcggt cggcatcggc gccaagcagg tgctctacaa cctgtgcgag gcactgctgg
     1201 acgagggcga cgagatcgtc ttcccgacgc cgtactggac ttcgtacgtg gacatcgcgg
     1261 agatcctggg ggcgcgcatc aatctgctgc cctgtccccc ggagcagaac tacaagctca
     1321 cgcctgcgca actggaccgc gcgctggcgg acaagcccaa ggtattcctg ttcaacaatc
     1381 cgtccaatcc gaccggcatg gtctacacgc gcgaggaaat cgccgcgctg gccgacgtga
     1441 tggcgaagca cccggacacc tggatcatcg ccgacgacat ctacaaccgc atggtgttcg
     1501 acggagtcgg ttaccacaac gtggtgcagt tccgccccga actgcgcgag cggaccgcga
     1561 tcgtggattc gctttccaag acctacggca tgcccggctg gcgcgtgggc ttcgtcgccg
     1621 cgcccgaacc gctggcgaac gcggtggtgg tactcaattc caaccacatc accaacatcc
     1681 cggaaatcgt caccgccgcc gcggtcgccg cgctgaccgg cccgcaggac atcaccaagg
     1741 aaaaatgcgc ggaattcgcc cgccgccgcg acgaggtgct gggggcgctg ctctcgatcc
     1801 cgggcgtgaa atgcccgcgc ccgcagggtg cgttctatgc gttcccggac atctcggttg
     1861 cgttcggcaa gtcgcacaac ggcacgttga tcgacaacga cgtggatttc tgcgccgcat
     1921 tgctggacgc caagggcgtc gccacggttc cgggctccgc cttcggggaa ccgcgtgcgt
     1981 tgcgcatctc ctacacctgt ccgcgcgcgc agctggaatc gggccttcaa cgcatcaagg
     2041 aattcttcgc ggaactggtc gaagtcgccc ccgtggcacg ggtcggcgcc gcaggggcgt
     2101 aatccgccgc cggcgattcg gcggggtgcg ccgccgtgcg gccagcccgc cctgcaaaag
     2161 cgcgtacgcg cgcaatcacg gatccaacaa ctgttcgagg tcaacgaatg aaagcacccg
     2221 ttcgcatcgc cgtcaccggc gccgccggcc agatcggcta cgcgctgctg ttccgcatcg
     2281 ccgccggcga catgctgggc aaggaccagc cggtcatcct gcacctgctg gaaatcacgc
     2341 ccgcgctgcc cgcgctgcag ggcgtggtga tggaactgga tgactgcgca ttcccgctgc
     2401 tgcagggcat cgtggcgacg gacgacgcga acgtcgcgtt caaggacgtg gattacgcgt
     2461 tgctggtcgg cgcg
//
LOCUS       DASSNH010000107         6361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1404927, whole genome shotgun sequence.
ACCESSION   DASSNH010000107 DASSNH010000000
VERSION     DASSNH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404927"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFK00_03790"
     CDS             complement(<1..323)
                     /locus_tag="VFK00_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592109.1"
                     /translation="MTKQTASIFVRFATSVLAGTLCAGAMAATPSAPAALVNPLIGSR
                     NGGNTFPGATLPFGMLQWSPENTRGKHGRTAAPGGYQYDATRIRGFSLTHLSGPGCAG
                     ASGDIP"
     gene            complement(588..1775)
                     /locus_tag="VFK00_03795"
     CDS             complement(588..1775)
                     /locus_tag="VFK00_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HET7592110.1"
                     /translation="MSDFPDFWSPDFLRAHIRETMAFYHPHCLDPAGGCFHYYSDDGA
                     IYDRSHRHLVSSARFVFDYAMYARRFGGAEYLDATRHCVAFIRDAHRNPATGGYVWTL
                     RDGHPEDTTNHCYGAAFVLVAYSQARMAGLIEAAAWMEEHWNLLEQRYWDADAGLYRD
                     EADADWNFSDYRGQNANMHMCEAMLTAFEASGEARWLDRAYTLADNMTRRQAAKAGGL
                     VWEHYDRAWEIDWEYHKDNPKDLFRPWGFQPGHQTEWAKLLLIMGRHRDADWLLPTAR
                     HLFDTAVARAWDPEYGGLCYGFAPDGSICDDDKYHWVQAESLACAALLAMRTGEEKYR
                     DWYRRLWEYAWTHFVDHVHGGWYRILTRDGRKYSDEKSPAGKTDYHNMGACYEVLGVA
                     ARA"
     gene            complement(1768..2742)
                     /locus_tag="VFK00_03800"
     CDS             complement(1768..2742)
                     /locus_tag="VFK00_03800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET7592111.1"
                     /translation="MNNVCCFGEALIDFHGTPSGAAPVFTAHPGGAPANVAVAVARLG
                     GPAAFVGMLGSDIFGDLLLRELVAAGADVAHARRTDAANTALAFVSHAPNGERDFSFY
                     RPPAADLLFRNEDFDPAIFAAGTIFHGGSCSMTEPDSAATTLQGMTRAREAGALASYD
                     MNLRPPLWPRGEDPAPTIWRALLLADFVKLSAEELAFLATSCGGEDAVLARLWAACAR
                     LVVITDGERTTRWLTPHASGEQGTFRVHAIDTTGAGDAFVGGCLYQLAKAGVDAAALS
                     AFAADAARLESMLRFASACGALAVTRAGSFVAMPALAEVEAFLEQQHE"
     gene            complement(2739..3944)
                     /locus_tag="VFK00_03805"
     CDS             complement(2739..3944)
                     /locus_tag="VFK00_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HET7592112.1"
                     /translation="MTTIFFMWGFITALNDVLIPHLKSVFTLNYAEVMLVQSTFFGAY
                     FIMSLPSGKVLARWGYRVSIIGGLIVTGIGALLFIPGAQLQSYPLFLFAFFVLASGIT
                     LLQVAANPYVSLLGPEHTASSRLNLAQALNSLGTTIAPKLGGLLILSSAVLGAAELAK
                     LSPAAQTAYKLQQAHSVQGPYIGIAIVLFLLAIVVWLFRMPQLEDTAEADDSRHRFAD
                     ALKLPRVWLGMLAIFVYVGAEVSIGSFMVNYLSLPEIGNMSEAAATGYVSLYWGGAMV
                     GRFIGSALLTRIGARKLLAFNATIASLLVITTMLTLGHVALWSVVAIGLFNSIMFPNI
                     FTLGIEKFGPLTGKVSSLLVMAIVGGAIIPLAQGALADHIGVHHAYVLPLVCYLYIIY
                     YGLRGSRVA"
     gene            4251..5702
                     /locus_tag="VFK00_03810"
     CDS             4251..5702
                     /locus_tag="VFK00_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET7592113.1"
                     /translation="MPDRRGFLKWIGAAAAAGTLGLPVFSGRAATPSSPRFASRRPPP
                     AHRKFTSPAVERELARVKGLIGDPKLAWLFENCYPNTLDTTVQTGTLDGKPDTFIVTG
                     DIDAMWLRDSSAQVWPYLPLAPHDPALRRLYRGLIHRHARCIAIDPYANAFMADPRAG
                     TNLPWAQHDQTDMKPGVAERKWEIDSPCWTIRLAHGYWRATGDRTPFDDDWRESMRSV
                     VATFRTQQRKHGPGPYRFQRETASPTDTLALGGYGFPAKPVGLIFSMFRPSDDACRYP
                     LFVPGNWFAVVSLRQLAAMMRQLFADGSFARDCEALADEVEAALRQYAPMQEPSGSAF
                     WAYEVDGYGNQLFMDDANVPSLLSLPYLGCCTRDNPAYRRTRALAWSDRNPYFFGGKA
                     VQGIGGPHEGLRMIWPMSIMIRALTSDDSREILQCLYWLKTTDAGTGFMHEAFDQDDP
                     SHYTRAWFAWANSLFGELIVALATHKPELLKRV"
     gene            5868..>6361
                     /locus_tag="VFK00_03815"
     CDS             5868..>6361
                     /locus_tag="VFK00_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET7592114.1"
                     /translation="MVTRRRFLQGAMALALLERIDPRAALAKGQQAGPTEAGPPPAGA
                     NKSQFVDAFVGTGGHGHTFPGATVPWGMVQLSPDTNDFGWDACSGYHQGDGSIMGFSH
                     THLSGTGASDMLDVLVMPAQGQVLLDAGARGLPDELYYSRYDAAARGSHLAPDVVANP
                     GKGYR"
BASE COUNT         1073 a         2047 c         2179 g         1062 t
ORIGIN      
        1 gggatgtccc cgctcgcccc cgcgcagccg gggccggaga gatgcgtcag gctgaagccg
       61 cggatgcggg tcgcgtcgta ctggtagccg cccggtgcgg cggtgcggcc gtgcttgccc
      121 ctggtgttct ccgggctcca ctgcagcatc ccgaacggca gcgttgcgcc cggaaaggta
      181 ttgccgccgt tgcggctgcc gatcagcggg tttacgagcg ccgcgggcgc ggacggcgtg
      241 gcggccatgg cgccggcgca gagtgttccc gccaacacgg atgtggcgaa gcgaacgaag
      301 atgcttgcag tctgtttggt catgacaagt acttcccggt gacgtcattc gatgccagcc
      361 cgacttttca ctcgcggggt tcgcttcgtg cgggcatccc cccgatgctt cgcatccgcg
      421 cttcgcgcgt cgctacgcat cgaccccctt cgttccgaag ggggtgaaac atcacgcgtg
      481 gcctccccct tcgcttgcga agggcggagc gtagcgaacg cgcgcatagc gcgagagggg
      541 gatggctctt cagcgcgtgg tggcatttcg ttccgctgca cgagggatca ggctcgtgct
      601 gccacgccga gcacttcgta acacgccccc atattgtggt aatcggtctt gccggcgggg
      661 cttttttcgt cgctgtactt gcggccgtcg cgggtgagga tgcggtacca gccgccgtga
      721 acgtgatcga cgaagtgggt ccacgcgtat tcccacaggc gccggtacca gtcccggtat
      781 ttttcttcgc cggtgcgcat tgccagcagc gccgcgcagg ccagcgattc ggcctgcacc
      841 cagtggtact tgtcgtcgtc gcagatcgac ccgtccggtg cgaacccgta acacaacccg
      901 ccgtactccg gatcccatgc gcgcgccacc gcggtgtcga acaggtgcct tgcggtcggg
      961 agcagccagt cggcatcgcg atggcggccc atgatcagca ggagcttggc ccattcggtt
     1021 tggtggccgg gctggaagcc ccaggggcgg aacaggtcct tcgggttgtc cttgtggtac
     1081 tcccagtcga tttcccacgc ccggtcgtag tgttcccaca ccaggccacc ggccttggca
     1141 gcctggcgcc gggtcatgtt gtcggcgagc gtgtacgcac gatcgagcca acgcgcttcg
     1201 ccgctggcct cgaatgcggt cagcatcgcc tcgcacatgt gcatgttggc gttctggccg
     1261 cggtagtcgg agaagttcca gtccgcgtcg gcttcgtcgc ggtacaggcc ggcgtcggca
     1321 tcccagtagc gctgctccag aaggttccag tgctcttcca tccacgcggc cgcttcgatg
     1381 aggccagcca tgcgcgcctg tgaataggcg accagcacga aagcggcgcc gtagcaatgg
     1441 ttggtggtgt cttccggatg gccgtcgcgc aaggtccaca cgtacccgcc ggtcgcggga
     1501 ttccggtgtg cgtcgcggat gaaggcgacg cagtggcggg tcgcgtcgag gtattcggcg
     1561 ccaccgaacc ggcgcgcgta catcgcgtag tcgaacacga agcgcgcgct cgagaccagg
     1621 tggcggtgcg agcggtcgta gatcgcgccg tcgtccgagt agtagtggaa gcagccgccg
     1681 gccgggtcga gacagtgcgg gtggtagaag gccatcgtct cgcggatgtg cgcgcgcagg
     1741 aaatccggcg accagaaatc gggaaagtca ctcatgctgt tgctccagga aggcctcgac
     1801 ttcggcgagc gcgggcatgg ccacgaacga tccggcgcgg gtgacggcta gcgcgccgca
     1861 ggccgaggcg aaccgcagca tggattcgag tcgggcggcg tccgcggcaa acgccgacag
     1921 cgccgcggca tcgacgcctg ccttcgcgag ctggtacagg cacccgccca cgaaagcatc
     1981 gccagcgccg gtggtatcaa tggcatgcac ccggaacgtg ccttgctcgc cgctcgcgtg
     2041 cggggtgagc cagcgcgtgg tgcgctcgcc atcggtaatg acgaccagtc tggcgcaggc
     2101 ggcccacagc cgcgcgagca cggcgtcctc gccgccacag gatgtggcga ggaacgcgag
     2161 ttcctcggca ctcaacttga cgaaatcggc gagcagcagt gcgcgccaga tcgtgggcgc
     2221 aggatcttcc ccgcgcggcc acagcggcgg gcgcaggttc atgtcgtagc tggccagcgc
     2281 gccggcctcg cgggcacgtg tcatcccttg cagggtcgtc gctgcggagt cgggctcggt
     2341 catgctgcac gaaccgccat ggaagatcgt gcccgcggcg aaaatcgctg gatcgaaatc
     2401 ttcgttccgg aacagcaggt ccgccgcggg cgggcggtag aaactgaaat cgcgttcgcc
     2461 attgggggcg tgcgacacga acgccagtgc ggtgttggcg gcgtccgtgc gacgcgcgtg
     2521 ggcgacgtcc gcgccggctg ccacgagctc gcgcagcagc aggtcgccga atatgtcgct
     2581 gcccaacatg cccacgaacg ctgcgggacc gccaaggcgc gccacggcca ctgcgacgtt
     2641 ggccggcgcc ccgcccgggt gggcggtgaa caccggagcc gcgccggacg gcgtgccgtg
     2701 gaagtcgatc agcgcctcgc cgaagcagca gacgttgttc atgcgacccg cgagccccgg
     2761 agcccgtagt agatgatgta gaggtagcaa accagcggca gcacgtaggc gtggtgcacg
     2821 ccgatgtgat cggccagcgc cccctgcgcg agcgggatga tcgccccgcc cacgatcgcc
     2881 atcaccagca ggctggatac cttgccggtg agcggcccga acttctcgat gccgagggtg
     2941 aagatgttgg ggaacatgat cgagttgaac aggccgatcg cgacgacgct ccacaatgcg
     3001 acatgtccca gcgtcagcat ggtggtgatc accagtagcg atgcgatcgt ggcgttgaag
     3061 gccagcaatt tgcgcgcgcc gatgcgtgtc agcagggccg agccgatgaa ccgcccgacc
     3121 atggcgccgc cccaatacag cgagacgtag ccggtcgcgg ccgcttcgct catgttcccg
     3181 atctcgggca acgaaaggta gttgaccatg aaactgccga tcgagacttc cgcgcccacg
     3241 tacacgaaga tcgcgagcat ccccagccat acccgcggca gcttcagcgc atcggcgaaa
     3301 cggtgcctgg aatcgtcggc ctcggccgtg tcttcgagtt gcggcatccg gaacagccac
     3361 accacgatcg ccagcaggaa cagcacgatt gcgatgccga tgtaggggcc ctgcacgctg
     3421 tgcgcctgct gcaacttgta ggcggtctgc gcggctggcg aaagtttcgc gagttccgct
     3481 gcgccaagca ccgcgctgga gaggatcaaa aggccgccca gcttcggggc gatggtggtg
     3541 cccagcgagt tcagtgcctg cgcgaggttc aggcggctcg aggcggtgtg ctccggcccc
     3601 agcaggctga cgtaagggtt tgcggcgacc tgcagcaggg tgatgccgct ggcgagcacg
     3661 aagaacgcga acaggaacag cggatacgac tgcagttgtg ctcccggaat gaagagcagc
     3721 gcgccgatgc cggtgacgat cagcccgccg atgatgctga cgcggtaacc ccagcgcgcc
     3781 agcaccttgc ccgagggcag cgacatgatg aagtacgcgc cgaagaaagt ggactggacg
     3841 agcatcactt cggcgtagtt gagcgtgaac accgacttga ggtgggggat cagcacgtcg
     3901 ttgagcgcgg tgatgaagcc ccacatgaag aagatcgtgg tcatcaccga cagggcgacg
     3961 cgggtgctgc tcacgcgttc gggcgcgccc ggcaggcggt cggtgacggg gttcggattc
     4021 atcggaatgg attcctgcag ggcggatttg ccgatgatgc cagcgcccgc agtgcatcga
     4081 tacaggccga acctcgcctg catccgcttt attacggtcg ccttcgcgat ggagcggcca
     4141 cggttcgcag ccgaatcggc cgggggggcc gctacagtgt gttcattggc ggcaggccag
     4201 gtggcgcgcc gtgggcgacc tcttgccacg ccgaacaggg aagcgagtgc atgcccgatc
     4261 gtcgcggttt cctgaaatgg atcggagcgg cggcggcggc cgggacactc ggcctgcccg
     4321 tgttctccgg tcgggcggcg acgccgtcgt caccgcgatt cgccagcagg cgcccaccgc
     4381 ccgcgcaccg caagttcacg agtccagccg tggaacggga gcttgcacgc gtcaaggggc
     4441 tgatcggcga tccgaagctc gcgtggctgt tcgagaactg ctacccgaac acgctcgata
     4501 ccacggtgca aaccggaacg ctggacggca agcccgacac cttcatcgtc accggcgaca
     4561 tcgacgcgat gtggctgcgc gactcctccg cgcaggtctg gccgtacttg ccgctggcgc
     4621 cgcacgatcc cgcgctgcgg aggttgtatc gcggtttgat ccatcgtcac gcgcgctgca
     4681 tcgcgatcga tccctacgcc aacgccttca tggccgatcc acgcgccggg acgaacctgc
     4741 cctgggcgca gcacgaccag accgacatga agccgggcgt cgccgagcgc aaatgggaaa
     4801 tcgattcgcc gtgctggacc atccggctcg cgcacggcta ttggcgtgcg accggcgatc
     4861 gcacgccctt cgacgacgac tggcgcgagt cgatgcgctc cgtcgtcgcg accttccgca
     4921 cgcagcagcg caagcacggc cccggcccgt accgtttcca gcgcgaaacg gcgtcaccca
     4981 ccgacaccct cgcgctcggc ggctacggat tcccggcaaa gccggtcggc ctgatctttt
     5041 cgatgttccg gccgtccgac gatgcctgcc gatatccatt gttcgtgccg ggcaactggt
     5101 ttgcagtggt gtcactgcgg caactggcgg cgatgatgcg ccagctgttc gccgatgggt
     5161 cgttcgcgcg cgactgcgag gcgttggcgg atgaagtgga agcggccttg cggcaatacg
     5221 cgccgatgca agagccatcc gggagcgcgt tttgggcgta cgaggtggac ggctacggca
     5281 accaattgtt catggacgat gccaacgtgc cgagcctgct ctcgctcccg tacctcggct
     5341 gctgcacccg cgacaatccg gcgtatcggc gcacccgcgc gctggcgtgg agcgaccgca
     5401 atccgtattt cttcggcggc aaggcggtcc aggggattgg cggcccgcac gagggcttgc
     5461 ggatgatctg gccgatgtcg atcatgatcc gcgcgttgac cagcgacgat tcccgcgaaa
     5521 tcctccagtg cctgtattgg ctgaagacca ccgatgccgg caccggcttc atgcacgagg
     5581 cgttcgatca ggacgatccg tcgcactaca cgcgcgcgtg gttcgcctgg gccaacagcc
     5641 tgttcggaga actgatcgtg gcactggcca cgcataaacc ggaattgctg aaacgtgtgt
     5701 gaaatgtccc tctcccttcg ggagagggtg ccgcctgctg agcgcccagc gctgggggaa
     5761 gggtacgatg tgaaagagtg ctgcaacttc gccgaaccct cacccgccct tcgggcaccc
     5821 tctcccgaag ggagaggggt tgattatcga tttcgaagga gcacgacatg gtgacacgca
     5881 gacggttcct gcagggcgcg atggcgctcg cattgctgga gcgcatcgat ccacgcgcag
     5941 cactcgcgaa ggggcagcag gcgggtccca ccgaggcggg accgccgccg gccggtgcca
     6001 acaagtcgca attcgtcgac gcgttcgtcg gcaccggcgg acatgggcac acgtttcccg
     6061 gggcgacggt gccgtggggc atggtgcagc tttcgcccga caccaacgat ttcggctggg
     6121 atgcctgctc cggttaccac cagggcgatg gttcgatcat gggcttttcg cacacgcacc
     6181 tttccggcac gggcgccagc gacatgctgg acgtgctggt gatgccggcg caggggcagg
     6241 ttttgctcga cgccggtgcg cgcgggttgc cggacgaact ctattactcg cggtacgatg
     6301 ccgcggcgcg cggatcgcac ctcgcgccgg acgtggtggc gaatcccggc aagggttacc
     6361 g
//
LOCUS       DASSNH010000108        11112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_641865, whole genome shotgun sequence.
ACCESSION   DASSNH010000108 DASSNH010000000
VERSION     DASSNH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641865"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFK00_03820"
     CDS             complement(<1..906)
                     /locus_tag="VFK00_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET7592115.1"
                     /translation="MAKAKTAYVCSDCGAEYPKWQGQCEACGAWNTLSAFVVQPAAKV
                     DANAGRRAGYAGADASRVKPLGDVAGEVEKRHLIRIGELDRVLGGGLVEGSVVLVGGD
                     PGIGKSTLLLQTLAALGERLAGLYVSGEESLAQLAARGHRLGLELAPLRALAETCIER
                     ILEHAQNEKPRVLVIDSIQTIWTETLPAAPGSVSQVRESAARLVRFAKETGTSVFLVG
                     HVTKEGGIAGPRVLEHMVDAVLYFEGESGSRFRVLRAFKNRFGAVNELGVFAMGDKGL
                     REVPNPSSIFLTGHQGATPGSAVMVT"
     gene            complement(906..1988)
                     /gene="alr"
                     /locus_tag="VFK00_03825"
     CDS             complement(906..1988)
                     /gene="alr"
                     /locus_tag="VFK00_03825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7592116.1"
                     /translation="MTRSATATIHLDALRANLTRVRSLAGDAKVMAVVKADAYGHGLE
                     RCARALAGADAFGVASIADGLRLRAAGHRQRIVVLSGPDAASDLAEMRRLRLEAVIHH
                     ESQLAMLEAEHGGEPLKVWLKLDTGMHRLGFSPERAPELYARLRALPAVDRGIVLMTH
                     FASSDEFDNELTARQTQRFFDATGDLPCERTLSNSAGLLGWPDARADWVRAGGLLYGL
                     SVVEGQTGADFGFASAMTLATRLVAVHRIARGERVGYGATWECPEDMPVGVAAIGYGD
                     GYPRSARSGTPVLVGDAAAAVIGRVSMDLLALDLRNAPTARVGDAVTLWGPELPVETI
                     AQHAGTIAYELTCGVTRRVLFAEDGT"
     gene            complement(2095..3486)
                     /gene="dnaB"
                     /locus_tag="VFK00_03830"
     CDS             complement(2095..3486)
                     /gene="dnaB"
                     /locus_tag="VFK00_03830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7592117.1"
                     /translation="MSAVFPEPKSAPRVDVLRVPPHSIEAEQSVLGGLMLVGEAWDQV
                     ADRVVEEDFYRREHRLIFRAIGELAMGGKPCDAVTLGEWFERNGETATIGGAAYLAEL
                     ANNTPSAANIKAYADIVRDKSVLRKLIDAGTRIAGDGFNPEGRNTPEILEAAEQLVFK
                     IAEAGARGRKQAVSVRQSVKEAVELLAKRYANRGQLNGLTTGFKDLDDLTSGLQEQDL
                     VIVAGRPSMGKTAFALNIAEAVAMRAKQPVLVFSMEMSASQLAFRLISSLGRISQKEL
                     RSGELAEEEWPRVTQAASLLSESKIFIDDTPALSPGELRSRARRMMREHGLGLVVIDY
                     LQLMQVPGNKENRATEISEISRNLKAMAKELNVPVIALSQLNRALEQRNDKRPVMSDL
                     RESGAIEQDADLILFIYRDEVYNKESNHKGIAEIIIGKQRNGPIDTVKLTFLGQYTKF
                     ENYAPEAYVGSLE"
     gene            complement(3701..4960)
                     /locus_tag="VFK00_03835"
     CDS             complement(3701..4960)
                     /locus_tag="VFK00_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7592118.1"
                     /translation="MDATHSDVLVLGGGVIGLSCAVALLRRGASVRVLERGAPGCGAS
                     HGNCGTLTPSHAIPLTVPGMPWKALHWMLRRDAPLYVNPRPDWARWRWLLGFARRCNL
                     GFAERAAIARAAILQRSWSLLPRMLVEENIDCEYAPAGNLMVYRDSRTLLADRKEIAW
                     LHRLGIAAAALRGSEVEVMEPALLPGVVGGILHADDAQLRPDKLVDGLARRVRERGGA
                     LEMQTTIDGFATAGARVERVHTSRGDFSADRVVLALGAWTPGIAARLDLPLPLQPGKG
                     YSITMSRPEPCPRHALVLREASVCVTAWGSGFRLGSTMEFSGYDERLNRTRLDALRRG
                     ARAYLRTPLGAELHEEWWGWRPMSVDEIPLIGPVARWNNLLLATGHGMLGVSMSAATA
                     ELVASMVAGETPVLDPLPYAPARFAGL"
     gene            5188..5478
                     /locus_tag="VFK00_03840"
     CDS             5188..5478
                     /locus_tag="VFK00_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592119.1"
                     /translation="MNTNTDTAFCSVPEILLMLRAPGAGWLATLLSALDEAGRDPDFN
                     AYHRAILARYLDEQTVPAAVAETARQRTAQFEETLTAQTRAADLLPERLAAC"
     gene            5611..6144
                     /locus_tag="VFK00_03845"
     CDS             5611..6144
                     /locus_tag="VFK00_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7592120.1"
                     /translation="MERRPLIAAGHRGRVLAGLLAALLLGACATVGPAPNPPQVPATV
                     ANNVLFRAIGLVGTPYHWGGNTPETGFDCSGLVAYVFRTAAGIALPRTSRAMAALDAP
                     TIPRKDLEPGDLLFFGRRHHVNHVAIYVGHGRFVNAPDRGGTVRLDELAGYYWRDHYL
                     FAKRVLTPRVRAALAAD"
     gene            6208..6612
                     /locus_tag="VFK00_03850"
     CDS             6208..6612
                     /locus_tag="VFK00_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7592121.1"
                     /translation="MQNFTLLIGRLGLALIFILSGIGKLEAYQATAKLLVAHALPPGL
                     LPLVIFAELGGGLAVLCGLLTRWAAVGLFVYTLLAAVLFHSNFADHGEWINFMKNVAI
                     AGGFLVLAAHGPGDLSIDAWRKRRKQRQKIFF"
     gene            6820..9012
                     /locus_tag="VFK00_03855"
     CDS             6820..9012
                     /locus_tag="VFK00_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592122.1"
                     /translation="MADGRGLLEELKRRHVWRVAIAYAVAGWLLVQIATQVFPFFGIP
                     DWAVRLVVILVVIGFPAAVIWAWIYELTPEGVRRTEPLDSPEARPAHEHRRIGHKLNA
                     VIVAVMVLAIALLGWRLYAARHAVPPRHAQVATARPATPAPINAPTPAAAQPIPAKSI
                     AVLPFENLSNDKNNDYFVAGMQDLILTKLADIGDLKVIARTSTARYASHPVDLQSVAR
                     QLGVATVLEGSVQKQGKQVLINVQLIDATTQAHLWAQDYTRTLDNVFGVEGEVAGKVS
                     AALDAKLSSTEAARMSAVPTRNEAAYDLFLRAEYEATRTQIVGNPTLIGAAIRLYRQA
                     IEKDPGFALAYARLSYVESAHAALGGEDDAAQSDARARADAAKALKLAPNLAAAQLAL
                     GYNLYWSRRDYEGALEAFATALALRPNDSGALEGQGLVQRRMGRFDEAIGSFQHAFTQ
                     DPRNSVLAQDVGDAYMMVSRYPDAERWLSRALTLNPGNIPGKEALAHSILFGSGDIPR
                     ALAAAAGDASRLKVGTRVPLLICQRQYREALDLLESTPLDIPPNVSATFLRYVVVGKP
                     QSEAWLFRLLGEPDRARPLYGRVLPLLRTQLKAQEDLDLARVWSRIAEAELGLGRTRE
                     GLEAIEKSQAIVSTSRDRAYAPRLMQINAAQYAQADRANLAVPLLAKALATPGIGSDY
                     SPVMLWLDPVWDPIRRNGRFRALLAQYAGYKPAVTYDTPSAAHGAAPL"
     gene            complement(9260..9757)
                     /locus_tag="VFK00_03860"
     CDS             complement(9260..9757)
                     /locus_tag="VFK00_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7592123.1"
                     /translation="MRAEKDSVVSFHYSVRDAADAASEPLDDSSKRGEPMLALLGHGQ
                     LVPGVEKALTGRGAGDKFEVDIPPADAYGEYQEGLLQRVPKKYFQDARKLKPGMTTVL
                     QTRDGGRRMVTVHKVGMSSIDVDHNHPLAGKTLHFHIEITDVRAAAPEELQHGHAHAG
                     DGHSH"
     gene            complement(9849..11099)
                     /locus_tag="VFK00_03865"
     CDS             complement(9849..11099)
                     /locus_tag="VFK00_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7592124.1"
                     /translation="MRVLDRTQRHTVAATFLGWTLDAFDYFLLVMVMPAVAKSFGVDV
                     PEVAFALLLTLAFRPLGALIFGRLADQYGRRRILMIDVTLYSVLGLACAFAPSLLALL
                     VLRSLFGIAMGGEWGTGASLAMESIPARSRGIVSGILQSGYPCGYLLCALAYGLLFDS
                     IGWRGMFALGILPALLVLYLRRSVPESPVFEQRTQAGVRRGVVEAMRGRWKLLAYMIV
                     TMAVFNAFSHGSQDVYPTFLKLQMHFDTHTTSLLTIILNLGALVGGLVFGQWSETIGR
                     RRAMSIACVLALPAIPLWSYGGSVLLLAIGAFWIQVAVQGAWGVVPGYLNELSPDDVR
                     GTLPGFAYQLGNLLVSWTAFAMAAIAHAHGGNYAIVQSIWIAVVAVLLAFLTWFGPES
                     RGARFGVTEEAGDATMRGSSAVAD"
BASE COUNT         1885 a         3895 c         3590 g         1742 t
ORIGIN      
        1 ggtcaccatc accgcgctcc cgggcgtcgc accctgatgg ccggtgagga aaatcgacga
       61 cggattcggt acctcgcgaa ggcccttgtc gcccatcgcg aacacgccaa gttcgttgac
      121 cgcgccgaag cggttcttga aggcgcgcaa cacccggaac cgcgaacccg attcgccctc
      181 gaaatacagc accgcgtcga ccatgtgttc gagtacacgg ggaccggcga tgcccccctc
      241 cttggtgacg tgtccgacca ggaacacgct ggttccggtt tccttggcga aacgcacgag
      301 gcgcgccgcc gattcccgca cctgcgacac cgaacccggc gccgcgggca gggtttcggt
      361 ccagatggtc tggatcgaat cgataaccag cacgcgcggt ttttcattct gcgcgtgttc
      421 gaggatccgt tcgatgcagg tttccgcgag cgcacgcaac ggcgcgagtt ccaggcccag
      481 ccggtgtccc cgcgcggcga gctgcgccag cgattcctca ccactgacgt acaacccggc
      541 gagccgctcg cccagcgccg ccagcgtctg caacaacaac gtggacttgc cgatgccggg
      601 atcgccgccc accagcacca ccgagccttc gaccaggccg ccgccaagta cccggtccag
      661 ctcgccgatc cggatcagat ggcgcttttc gacctcgccc gccacatccc ccagcggctt
      721 gacccgcgat gcatccgcac cggcgtagcc ggccctgcgt ccggcattcg catcaacctt
      781 ggccgccggc tgcaccacga acgccgacag cgtgttccac gcgccgcagg cctcgcactg
      841 gccctgccac ttcggatatt cggctccgca atccgaacaa acgtaggcgg tcttcgcctt
      901 ggccatcagg tgccatcctc ggcaaacaat acgcgccgcg tcacgccaca ggtgagttcg
      961 taggcgatgg tgccggcgtg ctgcgcgatg gtttcgaccg gcaattccgg tccccacagg
     1021 gtcacggcgt cacccacccg cgcagtgggg gcgttgcgca aatccagcgc cagcaggtcc
     1081 atggacacgc ggccgatcac ggcggcggcc gcatcaccga caagcaccgg tgtcccggaa
     1141 cgtgcgctgc gcggatagcc gtcgccgtag ccgatcgcgg cgacgccgac cggcatgtcc
     1201 tcgggacact cccaggtcgc gccgtagccg acgcgctcgc cgcgcgcgat tcgatggacg
     1261 gcgacgaggc gcgtcgcgag cgtcatcgca gacgcgaaac cgaaatcggc cccggtctgg
     1321 ccttccacca cggacaggcc gtacagcagg ccgcctgcgc gcacccagtc ggcgcgagcg
     1381 tcgggccagc cgagcaggcc tgcggaattg gagagtgtgc gctcgcaggg caaatccccg
     1441 gtggcgtcga aaaacctctg cgtctggcgc gcggtgagct cgttgtcgaa ctcgtccgaa
     1501 gacgcgaaat gcgtcatcaa cacgatgccg cgatcgaccg ccggaagcgc acgcagccgt
     1561 gcgtacagtt cgggcgcgcg ctctggcgaa aacccgagcc gatgcatccc ggtgtcgagc
     1621 ttcagccaca ccttgagcgg ttcgccgccg tgctcggctt ccagcatcgc cagctgcgat
     1681 tcgtggtgga tcacggcttc gagccgcagc cggcgcattt cggccaggtc gctggcagcg
     1741 tcgggtcccg acagcaccac gatccgctgg cggtgccccg ccgcgcgcag gcgcagccca
     1801 tccgcgatcg acgcgacgcc gaacgcatcc gcgcccgcca atgcccgtgc acaacgctcc
     1861 aggccgtggc cgtaggcgtc ggccttcacg accgccatga ctttcgcgtc cccggcaagc
     1921 gagcgcaccc gcgtgaggtt ggcgcgcagg gcatccagat ggatcgtggc ggtggcggag
     1981 cgggtcacgg aaaaccgggg tccggaaaac cggggtcaga gtgcaatttc gggcaggaac
     2041 tgccagtgca ccaagccctc cgacgaaatt gcactctgac cccggttttc gccttcactc
     2101 caggctcccg acgtacgcct ccggcgcgta gttctcgaac ttggtgtact ggccgaggaa
     2161 ggtcagcttc acggtgtcga tggggccgtt gcgctgcttg ccgatgatga tctcggcgat
     2221 gcccttgtgg ttggattcct tgttgtagac ctcgtcgcgg tagatgaaca ggatcaggtc
     2281 ggcatcctgt tcgatcgcgc ccgactcgcg caggtcgctc atcaccgggc gcttgtcgtt
     2341 gcgctgttcg agcgcgcggt tcaactgcga cagcgcgatc accggcacgt tgagttcctt
     2401 ggccatagcc ttgaggttgc gcgagatttc ggaaatctcg gtcgcgcggt tttccttgtt
     2461 gccgggcacc tgcatcagtt gcaggtaatc gatcacgacg aggccgaggc cgtgctcgcg
     2521 catcatccgg cgcgcacgcg aacgcagctc gccgggcgac agcgccgggg tgtcgtcgat
     2581 gaagatcttc gattccgaca gcagcgaggc tgcctgcgtc acgcgcggcc attcctcctc
     2641 ggcgagttcg cccgagcgca gctccttctg gctgatgcgg cccagcgagg agatcaggcg
     2701 gaacgccagt tgcgaggccg acatttccat cgagaacacc agaaccggct gcttggcgcg
     2761 catcgccacg gcttcggcga tgttgagcgc gaaggcggtc ttgcccatcg aggggcgtcc
     2821 ggcgacgatc accaggtcct gctcctgcag gcccgaggtc aggtcgtcga ggtccttgaa
     2881 gccggtggtc aggccgttca actggccgcg gttcgcatag cgcttggcca gcagctccac
     2941 cgcctccttg accgactggc gcaccgatac cgcctgcttg cgtccgcgcg cgccggcctc
     3001 ggcgatcttg aacacgagtt gttcggcggc ttcgaggatt tccggggtgt tgcggccttc
     3061 cggattgaac ccgtcgccgg cgatccgggt gccggcgtcg atcagtttgc gcaacaccga
     3121 cttgtcgcgg acgatgtcgg cgtaggcctt gatgttggca gcgctgggcg tgttgttggc
     3181 gagttccgcc aggtacgccg cgccgccgat cgtggcggtc tcgccgttgc gttcgaacca
     3241 ctcgcccagc gtcaccgcat cgcagggctt gcctcccatc gcgagttcgc cgatcgcgcg
     3301 gaagatcagg cggtgctcgc ggcggtagaa gtcctcctcc accacgcggt cggcgacctg
     3361 gtcccaggct tcgccgacca gcatcagccc gcccagcacc gactgctcgg cctcgatcga
     3421 atgcggcggc acccgcaaca cgtcgacgcg cggcgcggac ttgggttcgg ggaagacggc
     3481 actcatcggt gcgcggcggt cagcggcttc ggcatgccgg ggatcatacg cgcgcaggtc
     3541 tcaccgcacg atacgacaag tctgtggaaa tcctgtgcaa gattcgcggg acggcggaaa
     3601 gagcattgga cgcggatcga cgcggatcaa cacagacaaa aggtgtgccc ctgatccggg
     3661 cttgtccggt ttgatccgcg tcgaagggct tttgtcgaag tcacaaaccc gcgaagcgcg
     3721 ccggcgcgta gggaagcgga tcgagcaccg gcgtctcgcc ggcaaccatc gaggcgacga
     3781 gttccgcggt ggctgcgctc atgctcacgc caagcatccc gtgaccggtc gcgagcagca
     3841 ggttgttcca acgcgcgacc ggtccgatca gcggtatctc gtcgaccgac atcggccgcc
     3901 atccccacca ttcctcgtgc agttcggcgc ccagcggggt gcgcaggtac gcgcgcgcgc
     3961 cgcggcgcaa ggcatccagc cgcgtgcggt tcaagcgttc gtcgtacccc gagaattcca
     4021 tggtgctgcc gagccggaaa ccggagcccc aggcggtgac gcagacgctc gcctcgcgca
     4081 gcaccagcgc gtgtcgcgga cagggctccg gccgcgacat cgtgatcgaa tagcccttgc
     4141 ccggctggag gggcagcggc aagtcgagcc gcgcggcaat cccgggcgtc catgcgccga
     4201 gcgccagcac cacccggtcc gccgaaaaat caccacgcga ggtgtgcacg cgctcgaccc
     4261 gcgcacccgc ggtcgcgaat ccgtcgatcg tggtttgcat ctcgagcgcg ccgccgcgct
     4321 cgcgaaccct gcgcgccagt ccgtcgacca gcttgtcggg tcgcaactgc gcgtcgtccg
     4381 cgtgcaggat gccgccgacc acgcccggca gcagcgccgg ttccataacc tcgacctcac
     4441 tgccgcgcaa cgccgccgcc gcgattccca gccggtgcag ccaggcgatt tccttgcggt
     4501 ccgcgaggag ggtcctggag tcgcggtaca ccatcaggtt gccggcgggc gcgtattcgc
     4561 agtcgatgtt ttcctcgacc agcatgcgcg gcagcaacga ccacgaacgt tgcaggatcg
     4621 ccgcccgcgc gatcgccgcg cgttcggcga atcccaggtt gcaacgcctg gcgaaaccga
     4681 gcagccagcg ccagcgcgcc cagtccgggc ggggattgac gtacagcggg gcgtccctgc
     4741 gcagcatcca gtggagcgcc ttccagggca tgcccgggac ggtcaggggg atcgcgtggc
     4801 tgggcgtcaa ggtgccgcag ttgccgtgcg aggcgccgca acccggggcg ccgcgctcca
     4861 gcacccgcac cgacgcgccg cggcgcaaaa gggccactgc acaggacaaa ccgatcaccc
     4921 cgccgccgag gaccagtacg tcgctgtgtg tcgcatccat cccgaccatt ctagcgtcgg
     4981 ggcggacgcc gggcagccag gcagtcgtcg caggccggcc acgcatctgc ccgggcgcgt
     5041 cactttcagc cgctcccgca gcggatcccg cgcgtcgaaa gcccgcaccc cgcgggcatc
     5101 cacggcagcc gggaggcgcc gcaaggctcg cccgcatccc gtttggcatc attcctgctt
     5161 ggtatcccgt gaacagaggg aatccaaatg aacaccaaca ccgacacggc tttctgcagc
     5221 gttccggaaa tcctgctcat gctccgggcg cccggcgccg gctggctcgc gaccttgctg
     5281 tccgcgctgg acgaagccgg ccgcgacccg gatttcaatg cctatcatcg cgccattctt
     5341 gcgcgttacc tcgacgaaca gacggtgccc gcggcagtcg cggaaaccgc gcggcaacgt
     5401 acggcgcagt tcgaggaaac actgacggcg cagacgcgcg ccgccgacct gctgcccgag
     5461 cgcctcgcgg cctgctgagc cggccacccc gcgggcgctg gacgccgccc gcgccatggg
     5521 ttaccgtctg ggccgacctt gcgggagagc cctcgtccac ccgatgcctg cacgtgcctg
     5581 ccaagacccg ggccgttcca tgcggtccgc gtggagcgcc gtccgttgat cgccgcaggg
     5641 catcgcggcc gcgtactggc cggcctgctt gcggcgttgc tgctgggcgc ctgcgccacg
     5701 gtcgggcccg cgccgaatcc accgcaggta cccgcgaccg tcgcgaacaa cgtgctgttc
     5761 cgcgccatcg gcctggtcgg aaccccgtac cactggggcg gcaatacgcc ggaaaccgga
     5821 ttcgactgtt cgggcctggt cgcttatgtc tttcgcaccg cggccggcat cgcgctgccg
     5881 cgcacctcgc gcgcgatggc cgcactggat gcccccacca tcccgaggaa ggacctcgag
     5941 cccggcgacc tgctgttctt cggccggcgc caccacgtca accacgtcgc catctacgtc
     6001 ggccatggcc gcttcgtgaa cgctcccgac cggggcggca cggtgcggtt ggatgagctc
     6061 gccggctatt actggcgcga ccactacctg ttcgccaagc gggtgctgac gccgcgtgtc
     6121 cgcgcggcac tcgccgcgga ttgatccgcg cccggccgcg cccattgcac gggcaaccgg
     6181 gcgggcggaa aaccaacgga acccacgatg cagaacttca ctttgctgat cggccgactc
     6241 ggactggcat tgatcttcat cctgtccggc atcggcaagc tcgaagccta ccaggccacg
     6301 gccaagctgc tggtggcgca tgcgctgccg ccgggactgt tgccgctggt gatcttcgcg
     6361 gaactgggcg gcggcctcgc cgtgctgtgc gggctgctga cgcgctgggc ggcggtgggc
     6421 ctgttcgtct acaccctgct cgccgccgtg ctgttccaca gcaacttcgc cgaccacggc
     6481 gaatggatca atttcatgaa gaacgtggcg atcgccggcg ggttcctggt cctggccgcg
     6541 cacggtccgg gcgacctgtc gatcgacgcc tggcgcaagc gccgcaagca acgccagaag
     6601 atctttttct gaaaggctct cttgcgatcg tccctgatcg ccgcatcgtt catccgatct
     6661 gccaacgaca cccgttccgg tccctcaaca ccggaacggc catccatggc ctgactgttc
     6721 acaacagccg cgttgcacga aacaaaatcg ccgcttgaac ggcgatacac tgatgccgtc
     6781 ggaagccggc cggttgccgg cgggaacggc ggtgcaccag tggcggacgg tcgcggcctg
     6841 ctcgaagagc tgaagcggcg ccacgtgtgg cgggtggcga tcgcctacgc ggtcgcgggt
     6901 tggctgctgg tgcagatcgc gacgcaggtt ttccccttct tcggcattcc cgactgggcc
     6961 gtgcgcctgg tggtgatcct ggtcgtgatc ggcttcccgg ccgcggtgat ctgggcctgg
     7021 atttacgaac tgacgccgga aggcgtgcgt cgcaccgaac ccctcgattc gccggaagcg
     7081 cgtcccgcgc acgaacaccg ccggatcggg cacaagctca acgccgtcat cgtggcggtg
     7141 atggtgctcg cgatcgcgtt gctgggctgg cggttatacg cggcgcggca cgcggtaccg
     7201 ccacgacacg cgcaggtcgc gacggcgagg ccagcgacac ccgcgccgat caatgcgccc
     7261 acacctgccg ccgcccagcc gattcccgcc aaatccatcg ccgtactgcc gttcgagaat
     7321 ctctccaacg acaagaacaa cgattatttc gtcgccggca tgcaggacct gatcctgacg
     7381 aagctggccg acatcggcga tctcaaggtg atcgcgcgta cctccaccgc gagatacgcc
     7441 agccaccctg tcgacctgca atccgtcgcc cggcaattgg gggtcgcgac cgtcctcgaa
     7501 ggcagcgtgc aaaaacaggg caagcaggtg ttgatcaacg tccagttgat cgatgccacc
     7561 acgcaggcgc acctctgggc gcaggattac acgcgcaccc tcgacaacgt gtttggcgtc
     7621 gagggcgagg tggccgggaa ggtgtccgcg gcgctcgatg cgaagctttc ctcgacggaa
     7681 gccgcgcgca tgtctgcggt accgacccgg aacgaagccg cctatgacct gttcctgcgc
     7741 gccgaatacg aggcaacccg gacccagatc gtcggcaacc ccacgctgat cggagccgcg
     7801 atccggctgt accggcaggc catcgagaag gatcccggct tcgcgctcgc ctacgcgcgg
     7861 ctatcctacg tcgaaagtgc acacgcagcg ttgggcggcg aggatgatgc cgcgcaatcg
     7921 gatgcacgcg cacgcgccga cgccgcaaag gcactcaagc tggcgccgaa tctcgccgcc
     7981 gcgcaactcg cgttggggta caacctctat tggagccggc gcgattacga gggcgcgctc
     8041 gaggcttttg ccacggcgct cgcgcttcgc cccaacgata gcggtgcgct cgaaggacaa
     8101 gggctggttc aacgccgcat gggacgtttc gatgaggcga ttggctcctt ccaacacgca
     8161 ttcacccagg atccacgcaa ctcggtgctc gcccaggatg tcggcgacgc ctacatgatg
     8221 gtcagccgtt atcccgatgc cgaacgctgg ctgtcgcgtg cattgacgct gaaccccggc
     8281 aacattcccg gcaaggaggc tttggcccac agcatcctgt tcggcagcgg cgacattccg
     8341 cgcgcactgg ccgcggccgc gggcgatgcg agccgactga aagtgggaac gcgcgtgccc
     8401 ctgttgatct gccaaaggca gtaccgggaa gccctggacc tgctcgagag cacgccactg
     8461 gacatcccgc ccaacgtttc cgccacgttt cttcgttacg tggtcgtcgg caagccgcag
     8521 agcgaagcgt ggttgttccg gttgcttggc gagccggacc gagccaggcc cctgtatggc
     8581 cgcgtcctgc ccttgctcag gacccaactc aaggcgcaag aggatctcga cctggcaagg
     8641 gtgtggagcc gcatcgccga agccgaactg ggcctgggcc gcacccggga aggtctcgaa
     8701 gccatcgaaa agtcccaggc cattgtttcg accagcaggg accgcgccta tgcgccgaga
     8761 ctgatgcaaa tcaacgccgc gcaatacgcc caagccgacc gcgccaacct cgccgtgccg
     8821 ctgctcgcca aggcgctggc cacgcccgga atcggcagcg actactcacc cgtgatgttg
     8881 tggctggatc cggtctggga cccgatccgc cgtaacggac gcttccgggc gctgctggcg
     8941 cagtacgccg gatacaaacc ggcggtcact tatgacaccc catcggccgc gcacggagcc
     9001 gcgcctttgt aggttgggct gatcccgggt cgaagcccgg cggcgcagtc gcgtggcgat
     9061 gtcgagttcc ggagatgtag gagcgggcca cgcccgcgac caaggaaaac gcgacgcggc
     9121 ccgcgctacc gaaccacgcg acatgttggt cgggttgatg ccgggctccg cccgggagaa
     9181 ccccaacgtt gcagccaccg cgccgatgtt gggcttcctc cggatgaatc cggagtcaac
     9241 ccaacctgca cgaaggtgtt cagtgcgaat gcccgtcacc ggcatgcgcg tgcccgtgct
     9301 ggagctcctc cggcgcggcg gcgcgcacgt cggtgatctc gatgtggaaa tgcagggtct
     9361 tgccggccag cggatggttg tggtcgacgt cgatcgaact catgccgacc ttgtgcacgg
     9421 tgaccatgcg ccggccgccg tcgcgggtct gcaagacagt ggtcatgccg ggcttcagct
     9481 tccgggcatc ctggaaatac ttcttgggca cccgttgcag caggccctcc tggtattcgc
     9541 cgtaggcgtc ggcgggcggg atatcgacct cgaacttgtc gccggctccg cggccggtca
     9601 gtgccttctc gacccccggc accaattggc catggcccag cagcgcgagc atcggctcgc
     9661 cgcgcttgct cgaatcgtcg agcggctcgg atgccgcatc cgccgcgtcg cggacgctgt
     9721 aatggaaact gaccacggaa tctttttcgg cacgcatgac aatctcggga tggaggacgt
     9781 tggagcccgc ccgcgtcggg tcggggctgc ccgcgcaggg cgcgggcgga gtcgtggagg
     9841 gatgacgcct agtccgcgac ggcggacgaa ccgcgcatgg tagcgtcgcc cgcttcctcc
     9901 gtcacgccga aacgcgcgcc gcgcgactcc ggaccgaacc aggtcaggaa cgccagcagc
     9961 accgccacca ccgcgatcca tattgattga acgatcgcgt agttgccgcc gtgcgcatgc
    10021 gcaatcgcgg ccatcgcgaa cgccgtccac gacaccagca ggttgccgag ctggtaggcg
    10081 aaacccggca aggtgccgcg cacgtcgtcg ggcgaaagtt cgttcaggta accgggcacc
    10141 acgccccatg cgccctgcac cgccacctgg atccagaacg cgccgatcgc cagcagcaac
    10201 accgaaccgc cgtagctcca caacgggatc gcgggcaagg ccagcacgca ggcgatcgac
    10261 atcgcgcggc ggcggccgat cgtctccgac cattggccga acaccagccc cccgaccagc
    10321 gcaccgagat tgaggatgat cgtcagcagg ctggtggtgt gggtgtcgaa atgcatctgc
    10381 agcttcagga acgtcggata cacgtcctgc gagccatggc tgaaggcatt gaataccgcc
    10441 atcgtcacga tcatgtacgc gagcagtttc caacgcccgc gcatcgcttc caccacgccg
    10501 cgccggacgc cggcctgcgt gcgctgctcg aacaccggcg actccggcac gctgcggcgc
    10561 aggtacagca ccagcagcgc cggcaggatg cccagcgcga acatgccgcg ccagccgatg
    10621 ctgtcgaaca gcagcccata ggccagcgcg cacaggaggt agccgcacgg ataccccgat
    10681 tgcaggatgc ccgagacgat gccgcgcgag cgcgcgggga tcgattccat cgccagcgac
    10741 gcgccggtgc cccactcgcc gcccatcgcg atcccgaaca gcgagcgcag caccagcaac
    10801 gcgagcagcg atggcgcgaa tgcgcaggca aggcccagca cggagtacaa cgtgacatcg
    10861 atcatcagga tccgccggcg cccgtattga tcggccagcc gtccgaagat caacgcgccc
    10921 aacggccgga atgccagcgt cagcaacagc gcgaacgcga cttccggcac atcgacgccg
    10981 aacgacttcg ccaccgccgg catcaccatc accagcagga agtaatcgaa ggcatccagg
    11041 gtccagccga ggaacgtggc cgcgacggta tggcgctggg tgcggtcgag cacgcgcagg
    11101 gcggcgagtg cg
//
LOCUS       DASSNH010000109         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_54491, whole genome shotgun sequence.
ACCESSION   DASSNH010000109 DASSNH010000000
VERSION     DASSNH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54491"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2377)
                     /locus_tag="VFK00_03870"
     CDS             complement(131..2377)
                     /locus_tag="VFK00_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HET7592125.1"
                     /translation="MKCRAPVSLGLLALLACAPAPGLCATDPSFLLTATAEDFGAYFP
                     SYLANGYFSTMTSVRGTEPDRAYMVAFMDYTESDISRPAAIPGWSEIDYDPGTGWMNS
                     TRLDPEIFSGYSQTLDMHDGTLATHYRFTYAGKGTDVQVTTFVSQADPHLAATRLSIT
                     PQFDGKVRLRFPFRLWSGYQPRFPIASMTGDQMIDAVIASGQDLNDKPVPTPDRAPVW
                     YPGTTVVTGDGGDTQQRTLWLDGRAVNGLEMAEAAAIELPGDLKIESVKLEKTTGKLS
                     LDVVAEVRKGTRYTFTKYIAASREDWGGDARTDLELATKARATGFDKLLARHVAAWHD
                     LWKADIVVDRDEAVQRALHSDLYYLLSNTTVGTAWAMGACALTPNYAGHVFWDSDSWV
                     FPALLLLHPERAKSIVMFRHRTMQGARDRAKQYGVEGLMYPWESDPQKGVDVTPHFAY
                     GVFREIHVNADIAIAQWQYYLATGDTRWLKQYGWPVIQGVATFWKSRVTYDKAKDRYE
                     ILHVTSPDEAYDDVPNDSFTNAAAAKALRIATRAAALVGATADPEWTEIANKMYIPFD
                     AKAQRHYDFDPSVPHDKITWMGSSLAWLMYPNLDLAMSPDVRRNDFDFQLQALRTHGD
                     NPNEMMMVMLTVGAAELGDADGAGNWIERNLVGFLKPPFNVRSETATNNAGYILATSA
                     GFLQSFIYGLTGLRIDDKGLDAEYAPVLPPDWKSVTLKNVSFRGEHYDYTIRRGEDGK
                     ARLVRREL"
     gene            complement(2571..>3653)
                     /locus_tag="VFK00_03875"
     CDS             complement(2571..>3653)
                     /locus_tag="VFK00_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592126.1"
                     /translation="VVVANPMGFVDFNNNYNIVEAGTGTNKAFYLSDSWDVGPWLLQA
                     AGRVENIDVNQRTCNQSKIDLDGNPLTLYDNTTPVCNGTWDYEHYKKTHPSFTAGANY
                     RFSDHMSAYIRGNTGGHFDDFDNGIRGVGGNFAPMQKIENMEAGFKYGSSTMFIDVSI
                     YQRLFTGLQYTPTGPNGQPIVGAPISTYGANSHGLNLNASWSPIENFNLHLVAAYMNG
                     HYSHYDGCAAYTDINGNPQCAPINGAPLQRQPKLRYAFTPSYTLPTGWGSITGYLTYS
                     HVGQHYQDISALQPLGTYQTLDAGIVADVGTNWQFSLRGTNLTNEIGLTEGNSRKFGV
                     NTGIGGVIMARPLFGREVNFQAKYLF"
BASE COUNT          598 a         1139 c         1219 g          697 t
ORIGIN      
        1 tgcggtggcg gtgaggagga agctcggatc cgtggcggcc aacgccgtcg ggacgacggc
       61 ggcaagcacc agcagggcac taccagccag accccccttc gtgtgcctct ttcgagccga
      121 ccgcatcccg tcaaagctcc ctgcggacca gccttgcctt gccatcctcg ccgcgccgga
      181 tggtgtagtc gtaatgctca ccccggaaac tgacgttctt cagcgtcacc gatttccagt
      241 ccggcggcag caccggcgcg tattccgcat ccagtccctt gtcgtcgatg cgaagcccgg
      301 tcaggccata gatgaagctc tgcaggaacc ccgccgaggt cgcgaggatg tagccggcgt
      361 tgttggtcgc ggtttcgctg cggacgttga acggcggctt caggaagccc accaggttgc
      421 gctcgatcca gttgccggcc ccgtcggcat cgcccagctc ggcggcgccg accgtgagca
      481 tcaccatcat catttcgttc gggttgtcgc cgtgggtcct cagcgcctgc aactggaagt
      541 cgaaatcgtt gcggcgcacg tcgggcgaca tcgccaggtc caggttcgga tacatcagcc
      601 aggccagcga cgagcccatc caggtgatct tgtcgtgcgg caccgaaggg tcgaagtcgt
      661 aatggcgctg cgccttcgca tcgaagggga tgtacatctt gttcgcgatt tcggtccact
      721 ccggatcggc cgtcgccccg accagcgcgg cggcccgcgt cgcgatccgg agcgccttgg
      781 ccgccgcggc gttggtgaac gaatcgttcg gaacgtcgtc gtaggcttcg tcgggcgagg
      841 tgacgtgcag gatttcgtag cgatccttcg ccttgtcata cgtcacgcgg ctcttccaga
      901 aggtcgcgac gccttggatc acgggccagc cgtactgctt cagccagcgg gtgtcgccgg
      961 tcgcgaggta gtactgccat tgggcgatcg cgatatccgc gttgacgtgg atttcgcgaa
     1021 acacgccgta ggcgaagtgc ggcgtcacgt cgacgccttt ctgcggatcc gattcccacg
     1081 gatacatcag cccttcgacg ccgtactgct tcgcgcggtc gcgtgccccc tgcatggtgc
     1141 ggtggcggaa catcacgatc gacttggcgc gctcggggtg caacagcagc agcgccggga
     1201 atacccagga atcggaatcc cagaacacgt ggccggcgta gttcggcgtc agtgcgcagg
     1261 cgcccatcgc ccaggccgtg ccgaccgtgg tgttggacag gaggtagtag aggtcggaat
     1321 gcaacgcgcg ctgcacagcc tcgtcgcggt cgacgacgat gtcggccttc cagaggtcgt
     1381 gccacgcagc cacgtgacgc gcgagcagct tgtcgaagcc ggtcgcgcgc gccttcgtgg
     1441 ccagctcgag atcggtcctg gcatcgccac cccaatcctc ccgcgaggcc gcgatgtatt
     1501 tggtgaacgt gtaacgcgtg cccttgcgca cttcggccac gacatccagc gacaacttgc
     1561 cggtcgtttt ctccagcttc acgctttcga tcttcagatc gccgggaagc tcgatggccg
     1621 cggcttctgc catctcgagg ccattcaccg cccgaccgtc gagccagagc gttcgttgct
     1681 gcgtatcccc gccatcgccg gtgacgacgg tcgtgcccgg ataccacacc ggcgcccggt
     1741 ccggcgtcgg caccggcttg tcgttcaagt cctggccgct cgcgatcacg gcgtcgatca
     1801 tctggtcgcc ggtcatgctg gcgatgggaa agcgtggctg gtagccggac cagagtcgga
     1861 atggaaaccg cagccgcacc ttgccgtcga actgcggcgt gatggagagc cgggtcgccg
     1921 cgaggtgcgg atcggcctgg ctgacgaagg tggtgacctg cacgtccgtc cccttgccgg
     1981 cgtaggtgaa tcggtagtgc gtggcgagcg tgccgtcatg catgtccagc gtctgcgaat
     2041 accccgagaa gatttcggga tcgaggcgcg tggaattcat ccagccggtg cccgggtcgt
     2101 aatcgatttc gctccagccg ggaatcgcgg ccggccgtga aatgtcgctc tcggtgtaat
     2161 ccatgaaggc gaccatgtag gcacggtcgg gttcggtgcc gcgcaccgag gtcatggtcg
     2221 aaaaatagcc gttggcgagg tacgacggga aatacgcgcc gaagtcctcc gcggttgcgg
     2281 tcagcaggaa acttggatcg gttgcacaca ggccgggcgc cggcgcgcac gccaggaggg
     2341 cgagcaggcc gagggatacg ggcgcgcggc atttcatcgc aatactcctg atggtcgggc
     2401 ccatgccggc atcaacagtg gtgacgggac ggagggcgga cgcgtgcgca gcgtcccgat
     2461 ccttgccctg cacgaacgaa ggatgccgcg accgcgctgg ccgcagtcat ccccggacgg
     2521 cccggatcga tcatgcgcga tccgggccgc cggttggcat cgaaggcccg tcagaagagg
     2581 tacttcgcct ggaagttcac ctcgcgtccg aacaacggcc gcgccatgat cactcctccg
     2641 atgccggtgt tgacgccgaa cttccgcgaa ttgccttcgg tcaggccgat ctcgttggtc
     2701 aggttggtgc cgcgcaggct gaactgccag ttcgtaccga cgtcggccac gatgcccgca
     2761 tccagcgtct ggtaggtgcc cagcggctgc agggccgaga tgtcctggta atgctggccg
     2821 acgtggctgt aggtgagata gcccgtgatg ctgccccaac cggtcggcag cgtataactc
     2881 ggcgtgaacg cgtaacgcag tttcggctgg cgctgcagcg gtgcgccgtt gatgggggcg
     2941 cactgcgggt tgccgttgat gtccgtgtac gccgcgcagc cgtcgtagtg gctgtagtgg
     3001 ccgttcatgt aggcggcgac caggtgcagg ttgaagttct cgatcggcga ccacgaggca
     3061 ttgagattca gcccgtggga attcgccccg tacgtcgaga tgggcgctcc cacgatcggc
     3121 tggccattag gacccgtcgg tgtgtactgc aggccggtga acagccgttg gtagatgctg
     3181 acatcgatga acatcgtcga cgacccgtac ttgaagccgg cctccatgtt ctcgatcttc
     3241 tgcatcggcg cgaagttgcc accgacgccg cggatgccat tgtcgaaatc gtcgaagtgc
     3301 ccaccggtgt tcccgcggat gtaggcactc atgtggtcgc tgaaccggta gttcgcgccg
     3361 gccgtgaacg agggatgggt cttcttgtag tgctcgtaat cccacgtgcc gttgcagacc
     3421 ggcgtcgtgt tgtcgtacag cgtgagcgga ttgccgtcga ggtcgatctt ggactggttg
     3481 caggtgcgct ggttgacgtc gatgttctcg acgcggccgg ctgcctgcag cagccatggg
     3541 ccgacgtccc acgagtcgga caggtagaac gccttgttgg tgcccgtgcc ggcctcgacg
     3601 atgttgtagt tgttgttgaa gtcgacgaag cccatgggat tcgcgaccac gac
//
LOCUS       DASSNH010000110         5917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_416814, whole genome shotgun sequence.
ACCESSION   DASSNH010000110 DASSNH010000000
VERSION     DASSNH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416814"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VFK00_03880"
     CDS             complement(<1..1038)
                     /locus_tag="VFK00_03880"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7592127.1"
                     /translation="MKNLIGLLLLVFFVVGAARAQTAPVTATPSQHPVTTTQTGHFDI
                     EAATNAYMAELSPEQRARSDAYFEGGYWLILWDLLVALGVAWLLLGTRLSARMRNLAE
                     RITRWRWLQTAIFVIQYIVLTTLLTLPWNIYEGYFREHKYGLSNLTLGGWFGEQAKGL
                     IVGLILGTIALVVIYAVIRKATRTWWLWGAGVAIVFAIFVATIAPVYISPLFNTYKSL
                     PDSPLKVQILSMARANGIPATDVYWFDASRQSKRISANVSGMFGTTRISLNDNLLKRS
                     SPDEVKAVLGHEMGHYVLNHVYKGIVFFGIVIVLAFAFVRWGFGWAERRWGERWDVRG
                     VGDVAGLPLLAA"
     gene            1242..1703
                     /locus_tag="VFK00_03885"
     CDS             1242..1703
                     /locus_tag="VFK00_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET7592128.1"
                     /translation="MADGKASKSGKPAAKRIAGKAAGGFTKEEREAMRDRAREAKSGG
                     GESDVLAAIAKMPEPDRGMATRLHAIVKGSAPGLVPKTWYGMPAYADQDGKVVCFFRN
                     ASKFKDRYATFGFNDKAALDDGNMWPTAFALTKLTAAEEKQIAALVKKAAG"
     gene            complement(1740..2639)
                     /locus_tag="VFK00_03890"
     CDS             complement(1740..2639)
                     /locus_tag="VFK00_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592129.1"
                     /translation="MTTRSRGPSAGIDWLKHGITVGFRHPKPIFGGAALLLLVLLLPS
                     LITLPVQFLSASAAAPQRAAILLGIMAISMLIGLLIVPLYAGYLQVIDAAERGLPARA
                     RDIFGPYRQGEALPLIGYGLALILVYILMIGIVIAAAGSGIASWYVQVLSAQANHLPP
                     PGLPPGFGIAMALLTVMGLFIMGFYSISLGQIALRRRGVFNAIGDGLAGALKNLLPLL
                     GLAFGLMVAWVVAVIGIVIVGVVLVLLGKLVGAWLMVVLLVPLYIALILLMFAVMFGV
                     MYYLWRDVCGGDTESGAARAIIA"
     gene            2856..3383
                     /locus_tag="VFK00_03895"
     CDS             2856..3383
                     /locus_tag="VFK00_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592130.1"
                     /translation="MIYLCEREIGFDATEIGFPSVMGCRAIVLVTGGGLFGYHLNGTL
                     SQGKRTAFANFINGHVQGATAKRMLYAASAGAGLPQDHQELRDIAAALGYGGAIYWAS
                     LPGAGSLYVHYTDVNHNTCSITQRAWSDAVDNVIDNKAPYLGNDRAMANGAAPGNMFT
                     NLSTAGLQAVYPTQI"
     gene            complement(3478..3813)
                     /locus_tag="VFK00_03900"
     CDS             complement(3478..3813)
                     /locus_tag="VFK00_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HET7592131.1"
                     /translation="MSSNTSPDEARRRFLKTTLVAAAAVATGSALRQLRAADSEPHLT
                     DADPTAKAMDYVEDATTSKNALYKPGSTCANCQLYTGGAGGYGNCQLFPGKLVNASGW
                     CTSYTRKAP"
     gene            3966..5345
                     /locus_tag="VFK00_03905"
     CDS             3966..5345
                     /locus_tag="VFK00_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET7592132.1"
                     /translation="METSHVVCCERPRAARIVALVGVLLALSIVVPAARAVPAFARQM
                     HQPCTACHIGGFGPQLTPFGRQFKRLAYALHVGTDTKVPLSMMLVETFTKTLKAQTEP
                     PADGFGTNDNTEMQQASVFLAGRLANNLGVLAQATYSQNGHVLGWDNIDLRYGRNFTL
                     AGKPAIWGLSLNNNPSVSDVFNTAPAWQFPYLAPDLAPGAPAAPILMGGFGQQVVGLT
                     YYMQIDNRLDLEAGGYRSLSPAFLRRVNADFDGRIAGTAPYARAAYTWNLPSGNVELG
                     AFGFHAARGLAGTDALGDAVALPGPTDKFTDFGIDASWQLLNGSQHIVTFNGLYLAER
                     QHLDATFAAGGSSNRRDTLQALNINASYWYRNTWGATLGGFVNNGSSDLLLYGNEGSP
                     NTQGGIVELNWNPFGHAGSWGQPYANVRVGLQYTWYTRFSGLVSNVDGAGRHASDNDT
                     LYLYTWLAF"
     gene            complement(5376..5813)
                     /gene="trxC"
                     /locus_tag="VFK00_03910"
     CDS             complement(5376..5813)
                     /gene="trxC"
                     /locus_tag="VFK00_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HET7592133.1"
                     /translation="MSESLHVVCPHCQAVNRIPAERLGAAPVCGKCHRPLFEGKPVEV
                     DEAAFQRHLARDDIPVLVDFWAPWCGPCRMMAPHYAQAAAQLEPRVRLLKVDTQAQQA
                     LGARHDIRSIPTLALFRGGREVARQAGATTGAGIVQWARAHGA"
BASE COUNT         1076 a         1963 c         1964 g          914 t
ORIGIN      
        1 cgcggcgagc aacggcagcc cggccacgtc gccgacgccg cgcacgtccc agcgttcgcc
       61 ccagcggcgc tctgcccagc cgaagcccca gcgcacgaag gcgaacgcaa gcacgatgac
      121 gatgccgaag aacacgatgc ccttgtagac gtggttgagc acgtagtggc ccatctcgtg
      181 gccgagcacg gccttgacct cgtcgggcga actgcgcttg agcaaattgt cgttgaggct
      241 gatgcgggtg gtgccgaaca tgccgctgac gttggcgctg atgcgcttgc tctggcggga
      301 tgcatcgaac cagtacacgt cggtggcggg aatgccgttg gcccgcgcca tggacaggat
      361 ctgcactttc agcgggctgt cgggcagcga cttgtaggtg ttgaacagcg gcgagatgta
      421 gaccggcgcg atggtcgcga caaagatcgc gaacacgatc gccactccgg cgccccacag
      481 ccaccaggta cgcgtggcct tgcggatcac cgcatagatg acgaccagcg cgatggtgcc
      541 gaggatcagc ccgacgatca gtcccttcgc ctgctcgccg aaccagccgc ccagggtgag
      601 attcgacaag ccgtacttgt gttcgcggaa gtagccttcg tagatattcc atggcagcgt
      661 cagcagcgtg gtcagcacga tgtactggat cacgaagatc gccgtctgca accagcgcca
      721 gcgggtgatc cgttcggcga ggttgcgcat gcgcgccgac agccgcgtgc cgagcagcaa
      781 ccacgccacg ccgagcgcca ccagcagatc ccacaggatc agccagtagc cgccctcgaa
      841 atacgcatcg gacctggccc gctgttcagg cgacaattcg gccatgtagg cgttggtggc
      901 ggcctcgatg tcgaagtggc ccgtctgcgt cgtggtgacc ggatgttgcg atggcgtggc
      961 agtgacgggt gcagtttgcg cccgcgccgc acccacgacg aaaaagacca gcaacagcaa
     1021 accgatcagg tttttcatgg aagcccccat acccgaaaga agccgcaatc tacccgaacc
     1081 ggagttcgtg cggaaaccgg ggcagggcca ttgcttcgta agccgctggt ttcctcacgc
     1141 aaacgatccc gtcctggctt cgccgtcgcg caggttccct tgttgcgccg caaccgccgc
     1201 gcgcggcact atccgaaccc cgcgaatgga aggaacagat catggccgac ggaaaagcaa
     1261 gcaagtccgg caagccggcc gcgaaacgca tcgcgggcaa agctgcgggt ggattcacga
     1321 aggaagaacg cgaggcgatg cgcgaccgcg cgcgcgaagc gaagtcgggc ggcggcgaaa
     1381 gcgacgtgct cgcggcgatt gcgaaaatgc cggaacccga tcgcggcatg gccacgcggc
     1441 tgcacgcgat cgtcaagggc agcgcgcccg ggctcgtccc caagacctgg tacggcatgc
     1501 ccgcgtatgc ggatcaggac ggcaaggtcg tctgcttctt ccgcaacgcg tcgaagttca
     1561 aggatcgcta cgcgaccttc ggtttcaacg acaaggcggc gctcgacgac ggcaacatgt
     1621 ggccgaccgc ttttgcgctc acgaagctga ccgccgccga agaaaaacag atcgccgcgc
     1681 tggtgaagaa ggcggcgggg tgaccgtcgc cgcctccgca tgagggcgat cgactcggct
     1741 caggcaatga tcgcccgcgc tgcgccggat tcggtgtcgc cgccgcacac gtcgcgccac
     1801 aggtagtaca tcaccccgaa catgacggcg aacatcagca ggatcagcgc gatgtaaagt
     1861 ggaaccagca acaccaccat cagccaggca ccgacgagct tgccgagcag cacgagcacc
     1921 acccccacga tcacgatgcc gatcaccgcg acgacccagg cgaccatcag gccgaacgcg
     1981 agcccgagca gcggcagcag gttcttcagc gcgcccgcca ggccgtcgcc gatggcgttg
     2041 aacacgccgc ggcggcgcag cgcgatctgg ccgaggctga tcgaataaaa ccccatgatg
     2101 aaaagaccca tcaccgtgag caacgccatg gcgatgccga agcccggggg caatcccggc
     2161 ggtggcagat ggttggcctg cgcggagagg acttgcacgt accagctggc gatgccgctg
     2221 cccgccgccg cgatgacaat cccgatcatg aggatataga ccaggatcaa cgccaggccg
     2281 taaccgatca ggggcaaggc ttcgccctgg cggtacggcc cgaagatgtc gcgtgcgcgc
     2341 gcaggcaacc cgcgttcggc cgcgtcgatc acctgcaggt aacccgcata cagcggcacg
     2401 atcagcaagc cgatcagcat ggagatcgcc atgatcccga gcaggatggc tgcgcgctgc
     2461 ggggcggccg cgcttgccga aagaaactgg acgggcagcg tgatcagcga gggcagcagc
     2521 aacacgagca gcagcagtgc cgcgcctccg aagatgggct tcggatggcg gaaacccacg
     2581 gtgatgccat gcttcagcca gtcgatgccg gccgatggtc ccctggatcg tgtcgtcatg
     2641 gtgcggcttc cccgatgatc ggcgcgcccc cgcgccatgc ggttcgagca tagcgcgcga
     2701 ggagaaccgg cagaagcggt tcgaggaagc ccccccggct tgcgcgcgca tgcgggatgc
     2761 ggcgcgacgg tttcggtcac agccgttcga agggatgcta taaacgcatc cggctgcggc
     2821 ggcctcgcga tccggggcac ggggagtgtc atgtcatgat ctatctgtgc gagcgtgaga
     2881 tcgggttcga cgcgaccgag atcgggtttc cttcggtcat gggttgccgc gcgatcgtgc
     2941 tggtgaccgg cggcggcctg ttcgggtatc acttgaacgg caccctgagc caggggaaga
     3001 gaacggcgtt cgccaatttc atcaacggcc acgtgcaagg cgccaccgcg aaaaggatgc
     3061 tgtatgcggc gtccgcgggc gcggggttgc cgcaggacca ccaggagctc agggacatcg
     3121 cggcggcgct cggctacggc ggagcgatct actgggccag ccttccgggc gcgggttcgc
     3181 tgtacgtgca ttacacggac gtcaaccaca acacctgctc gatcacgcag cgagcctgga
     3241 gcgatgccgt ggacaacgtc atcgacaaca aggcgcctta cctcggcaat gatcgcgcga
     3301 tggccaatgg tgcggcgccg ggcaacatgt tcaccaacct gagtaccgcc ggattgcagg
     3361 cggtgtaccc gacgcagatc tgagccgcgc aacgccggtt cggggtacgc ccggcgcggt
     3421 ggaagcaatg tcgccgttgc gcgctccccg cgcgacttca gtttcgctct cgtgcgctca
     3481 cggtgccttg cgcgtgtacg aggtgcacca gccgcttgcg ttcaccagct tgccggggaa
     3541 caactggcaa ttgccgtagc cgcccgcgcc gccggtatag agctggcaat tggcgcaggt
     3601 gctgccgggc ttgtagagcg cgttcttcga agtcgtcgcg tcctcgacgt aatccatggc
     3661 cttggcggtg gggtccgcgt cggtgaggtg cggttcgctg tcggcggcac ggagctgccg
     3721 cagcgcgctt cccgtcgcca ccgccgcggc ggcgaccagg gtcgttttca ggaagcgccg
     3781 gcgcgcttca tcaggcgatg tgttgctgga catgggaggt ctcccgctgg cacccccaat
     3841 gtgccgcaaa gatcccgccc cggattgacc catgtcaaag ggcgccgctt ttttcagggc
     3901 aatgctgcac accgcgcgag ggcaagttcc gagttggcgt cacggtccat cctggaggac
     3961 gcgtcatgga aaccagccac gtcgtttgtt gcgagaggcc gcgggcagcg cgcatcgtcg
     4021 cattggtggg tgtattgctt gccttgtcga tcgtcgtgcc ggccgccagg gcggtgccgg
     4081 ccttcgcacg ccagatgcac cagccgtgca cggcctgcca catcggcggc ttcggtccgc
     4141 agctcacgcc attcgggcgc cagttcaagc gcctcgccta cgcgctccat gtcggcaccg
     4201 ataccaaggt gccgctgtcc atgatgctgg tggaaacgtt caccaaaacc ctgaaggcgc
     4261 agaccgagcc gccggcggac ggcttcggca ccaacgacaa caccgaaatg cagcaggcgt
     4321 cggtgttcct tgccggccgc ctcgcaaaca acctcggggt gctggcgcag gcgacctatt
     4381 cccagaacgg ccacgtgctg ggatgggaca acatcgatct tcgctacggg cgcaacttca
     4441 ccctggcagg caagcccgcg atctggggcc tgtcgctcaa caacaacccc agcgtgagcg
     4501 atgtgttcaa taccgcgccc gcgtggcagt tcccgtatct ggcgccggac ctggcgcccg
     4561 gcgcacccgc cgcgccgatc ctgatgggcg gcttcgggca gcaggtggtc gggctcacct
     4621 actacatgca gatcgacaac cgcttggacc tcgaagccgg cggttaccgc tcgctgtcgc
     4681 cggccttcct gcgccgcgtg aatgcggatt tcgacggacg catcgccggc accgcgccct
     4741 acgcgcgtgc ggcctacacg tggaacctgc cgagcgggaa cgtggaactg ggcgccttcg
     4801 gcttccacgc cgcgcgcggc ctggcgggca ccgatgcgct cggcgacgcg gtggcgctgc
     4861 ccgggcccac cgacaagttc accgacttcg gcatcgatgc gagttggcaa ctgctcaacg
     4921 gcagccagca catcgtgacg ttcaacggct tgtacctggc cgagcgccag catctcgacg
     4981 ccacctttgc cgcaggcgga tcttccaacc ggcgcgacac gctgcaggcg ctgaacatca
     5041 acgcgagcta ctggtaccgc aacacctggg gcgcgacgct cggcggcttc gtcaacaacg
     5101 gcagcagcga cctgctgctc tatggcaacg agggcagccc caatacccag ggcggcatcg
     5161 tcgagctgaa ctggaatccg ttcgggcacg caggctcctg gggacagcct tacgccaatg
     5221 tccgcgtcgg cttgcagtac acgtggtaca cgcggttcag cggcctggtt tccaacgtgg
     5281 acggcgcggg acgccacgct tccgacaacg acacgctgta cctgtacacg tggctcgcct
     5341 tctagcgcgg ggctgacgct ccgcgaccgg gcacctcaag cgccatgcgc gcgtgcccac
     5401 tggacgatgc cggcgccggt cgtggcgccg gcctggcgcg ccacttcgcg gccgccgcgg
     5461 aacaacgcga gcgtcgggat gctgcggatg tcgtggcgtg cgccgagcgc ctgctgcgcc
     5521 tgcgtgtcaa ccttgagcag gcgcacgcgc ggttccagtt gcgcggcggc ctgcgcgtag
     5581 tgcggcgcca tcatccggca cggaccgcac cacggcgccc agaaatccac cagcacggga
     5641 atgtcgtcgc gcgcgaggtg cctctggaac gcggcttcgt ccacctcgac cggcttgcct
     5701 tcgaacagcg gtcggtggca tttgccgcac accggcgccg cgccgaggcg ctcggccgga
     5761 atccggttca ccgcctggca atgcgggcag acgacgtgca gggattcgct catcggaaca
     5821 cctccgggat cgtgcggtcg cgcttttgac ctgccgccga tatggaggcg gaaatgcggc
     5881 agggaagggc ggcgggtccg gcggcgcgcg cacgcgc
//
LOCUS       DASSNH010000111        12151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_16770, whole genome shotgun sequence.
ACCESSION   DASSNH010000111 DASSNH010000000
VERSION     DASSNH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16770"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFK00_03915"
     CDS             complement(<1..708)
                     /locus_tag="VFK00_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HET7592134.1"
                     /translation="MVAQPSTEHAGSGANDAEARVCALLVQRGRLKHDDLARTERLLA
                     EAPETHLTALLARLGLVSERDLAEAWSETLGLPLLSVRDAPDAPPETALTVRFMKQYH
                     VVPVGETDTALQLATADPGDAYIAHAVQLAAGRPVALVVALRAEIDDLIERWYGTGRS
                     ALGTIVEGLEAGDAAASGDDDIEHLRDLASEAPVIRLVNLLIQRAVEQRASDIHIEPF
                     ENRLKVRYRVDGVLHEVE"
     gene            complement(1017..1430)
                     /locus_tag="VFK00_03920"
     CDS             complement(1017..1430)
                     /locus_tag="VFK00_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592135.1"
                     /translation="MHRIYPWTGVAIAALLAGVAPVVLAQTNPASEDHPQSGAVTHTP
                     PFQTPPPQDNTPEQNPDQTSMPPRTPQSQTGQEMGQHDMRGTVESVSQDGIVKVRTDE
                     GTLKVHFPQARQHLKKGDTITLHLSYTIDSPPSSM"
     gene            complement(1638..2213)
                     /locus_tag="VFK00_03925"
     CDS             complement(1638..2213)
                     /locus_tag="VFK00_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592136.1"
                     /translation="MTQTAQISINTNLRPVESLGDDGYMNAYDVVNATQNKDAVKNWF
                     FTTLFATSNTKGVPYVSPALAAAVAALLNDINSGVLSFDFSLTMVFVFHDGSKVTFHY
                     NPQDGTLSYVVGQSRDGHGNLIPDNYQAITGGGRDVYDFTNPYPSYDAHNFQELLQDY
                     GIQPTGSTTAVLECVSVGGDQPTCHYVTVPR"
     gene            complement(2658..4040)
                     /locus_tag="VFK00_03930"
     CDS             complement(2658..4040)
                     /locus_tag="VFK00_03930"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HET7592137.1"
                     /translation="MAVSVFDIFKVGIGPSSSHTIGPMRAAARFVERWLADTGDLARV
                     ARVTVELHGSLAMTGRGHGTDKAVLCGLEGNWPDRIDPDAIPPLLERIRSDKRVRLGA
                     THEIAFDEKTDLVWNKRQKLPFHVNGMRFSAFDAEGKAIVSRDYYSVGGGFVVNADEA
                     AADRIVADTTPLPFPFRSGDELMALCDRHGMGIAQLMLENEKAWRSEAEIRAGLLEIW
                     KAMQDCVARGLRSPGELPGGLHVKRRAPAMYDELKERPEAALKDPLTVLDWVNLYALA
                     VNEENAAGGRVVTAPTNGAAGIVPAVLHYYDRFCPKSNEQGVIDFLLTAGAIGILYKE
                     NASISGAEVGCQGEVGVACSMAAGALVAALGGTHHQVENAAEIGMEHNLGLTCDPIGG
                     LVQIPCIERNAMGSVKAINAQRMAMKNDGKHHVSLDKVIRTMRDTGRDMKDKYKETSR
                     GGLAVNVVEC"
     gene            4232..4456
                     /locus_tag="VFK00_03935"
     CDS             4232..4456
                     /locus_tag="VFK00_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET7592138.1"
                     /translation="MLTLYQRDDCKLCDEAVALLARVRAPEFQSVWIDGDAGLEARYG
                     VRVPVLRDEASGRELGWPFDAEALRLFLDA"
     gene            complement(4499..5074)
                     /locus_tag="VFK00_03940"
     CDS             complement(4499..5074)
                     /locus_tag="VFK00_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7592139.1"
                     /translation="MKLLLAGMCTALVLGFAAPGMAKTWQVDMAHSSLTFTNTYQDVE
                     YTGRFRRFDAKIAYDPGDLAKAKFDVAVDIGSLDTRNEERDHTALGGDFFDAAKFPKA
                     YFATTTFRKGADGKVVALGELNLRGVVKPVQLAVTFTPHGDTATLDVTARLKRLDFGI
                     GTGQWADPSMIGNDVTVHGHLQLRATTTPDQ"
     gene            complement(5071..5643)
                     /locus_tag="VFK00_03945"
     CDS             complement(5071..5643)
                     /locus_tag="VFK00_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HET7592140.1"
                     /translation="MPLRSTEAGWGALVRAFHWLVAALILAQAVIGLSMIQMGLTPAK
                     VRVFALHKSIGMTILALVLLRIAWRLGERRPDDPAGMPRWQRGAARAVHLALYVLILA
                     IPLSGWWFNSASNAPLVWFGLFDIPSLTGGYDPVWKPRALLAHQTLFWILVALLVAHV
                     GAALWHHFRQRDEVLRRMVLGPRPRNGGGR"
     gene            complement(5624..6298)
                     /locus_tag="VFK00_03950"
     CDS             complement(5624..6298)
                     /locus_tag="VFK00_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7592141.1"
                     /translation="MSDETEVSVRRCLTSLLWLVLLAAGEAAAHPLDYRIDTVHSQVL
                     FSADHDGYSRPVGRLAIARGWLRFDPDDWDASKVVADIDLASADLGDGGWNAAVTGRN
                     FLDAAQFPSAHFQSTAVRKTGEHGGTLDGELTLRGVTLPVTVDFTVNRVGVTLFGFET
                     IAGFSGSANLDRTKFGMTAFPKSVGIEVAIRLEIEAKLDRSAEFDYRQAAEKAARSKA
                     HAAAQH"
     gene            6378..9971
                     /locus_tag="VFK00_03955"
     CDS             6378..9971
                     /locus_tag="VFK00_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7592142.1"
                     /translation="MLTALILAGALGTAAPAAAAGVPPTPIFRSYGVADGLPSANAYA
                     ITQDHAGFLWIGTHDGLVRYDSREFRVFRHEPGQPASLPANDVSALLVDRDGRLWGGG
                     EGTGLNRYEPATGGFRHWLHAADRPGSLSGNDVMAIAQDDAGAIWVGVYAGGLDKLDA
                     DGKGFTHLRHREDDPSSIVSNNVTALAPAADGGLWVGTDAGLDRLDANGRIHHVRLPG
                     FSTQPTIWQVRVASTGVDAATSAGLFHLDGKGGASLLGTARETFASLRDPDGSLWIAQ
                     RGGLARIDADGSTRFEQPTEGVTGSLPGRLPDGLFRDHEGGLWVALVDGGLAYLPPQW
                     RAFSMFRHIPGDAASLARDRVRALALAGDGTLWIGGAALLDRIDLADGKVSHTDIPGL
                     ARMSISALAVDPAGRLWIGSSRGLFLRDQGRLLPIAEGDPGLRHGVWRLLAARDGAIY
                     CAGVGTGVFRVDLGDRAIRPVAPPGKGENAQEIVQLREAGDGVIWAASKAGLAWLAPG
                     AAGFAFVPGIARGKVLGFAFGPGATVWVARDDRLQQYRLDHGRAKLVSETGHDEGWPD
                     TGVLALETGPDGRVYALGTRNLLVYGPRDHQLHAFTTADGLANTDFTSSVLLRAGDEL
                     FAGGPAGLVGVRVGELPQHLATPKVALESVTVHRDGRAQKLDPAGGIDLDWRDRELTV
                     TTQVLSFVDPARNRYRFRLQGFDHGWVETGSRNVREFSVLEPGDYRLLVSGRIGDGPW
                     SAPFEAFSLRVAAAPWTTPWAWALYVLAAVALLAWFVWMLRRRLQQQHRLALSEERQL
                     IAEQANAAKSRFLANMAHEIRTPLTGVLGMTELLLHTGLDQRQHRYADAIRRSGALLL
                     RHVNDALDVARIEAGRLELGVAPFDPAAVLREVAEADAGLAAQKGLRIDVEIAPDAPS
                     AVRGDAQRVQQVLFNLAHNALKFTAHGGIRLKLERVDDDIVYSVIDRGRGMTPEECAR
                     VFQRFEQADQGRLQRGSGLGLAISRELVNLMGGRIGVQSVPGYGSTFSVRLPLPIVAV
                     AQGDRAASAAGATSAAHRDTQPVATREAAESTTSPGHRVLLVEDDPVTGEALAGLIEA
                     FGHQVTLAPQALAALSEIDAGGSFDAMVFDFDLPGMDGCELAALLRQRGVATPIVALT
                     ASAHGDEEERARAAGMDAFLRKPVLPGPLHEALDAVLAETSVAGERPNTLL"
     gene            complement(10063..>12151)
                     /locus_tag="VFK00_03960"
     CDS             complement(10063..>12151)
                     /locus_tag="VFK00_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592143.1"
                     /translation="AYVAAAFALIQVLEVVADSYAWPHAVMHAAFGVLALGFAIALVL
                     AWYHGERGEDRISGPELLLIALVLAIGGGLLWHFGRSGSPATAPGNAVAAARGPDAAQ
                     RNPASAVPTSAAGAAAPGLRTTPVRAAQPIPAKSIAVLPFENLSNDKDNAYFVAGMQD
                     LILTKLADIGDLKVIARTSTEKYQSRPDDLEAIGRQLGVATILEGSVQKAGDEVLINV
                     QLIDAKTDAHVWAQDYQRKLADIFGVEGEVAGKIAVTLDAKLSPAETARLDAVPTTDQ
                     AAYDLFLRADYDANRGDTNYDTASWKAAIPLYRQAIARDPGFALAYARLSYVESILGW
                     FGGGGEDPGQLTAQASTDAKQALKLAPDFAASRIAMGYSDYYGARDYDGALDAFDAAL
                     AVRPNDADALAARGYVERRQGRFGDAITSLTGALAHDPRNGSLAYEIGYTCMMAARYA
                     DAEPWFERALALDPTNLNAKVWRSQAILLDSGDIAKALAAVAGDAPALEIQRVSLLSM
                     QRKDHDAIALLESVPDSPDVFSVGIGSKALLLADLYRLSGDTARARPLFSRALVTART
                     QLERQQNLKLAGAWQHVASAELGLGRTAAGLDAIGRAQAIVAATKDQTNGPVMMKRDA
                     ALYAEARRPDRAVPLLAKALAKRGISYVYSPLLLWLDPTWDPIRRDPGFRALLAKYAT
                     YKPAVHQGSSATP"
BASE COUNT         1907 a         4085 c         4083 g         2076 t
ORIGIN      
        1 ttcgacctcg tgcagcacgc cgtccacgcg gtagcggacc ttcagccggt tctcgaacgg
       61 ttcgatgtgg atgtccgagg cgcgctgctc gaccgcgcgc tggatcagga ggttcacgag
      121 ccggatcacc ggcgcttcgg aagcgaggtc gcgcaggtgc tcgatgtcat cgtcgcccga
      181 cgctgccgca tcgcccgctt ccaggccctc gacgatggtg ccgagggcgc ttcgccccgt
      241 gccataccag cgctcgatga gatcgtcgat ttccgcgcgc aacgccacga ctagggccac
      301 gggccggccc gcggcgagct gcaccgcgtg cgcgatgtag gcatcgccgg gatcggcggt
      361 tgcaagctgc agcgccgtgt ccgtttcgcc gaccggcacc acgtggtact gcttcatgaa
      421 gcgcacggtc agcgcggttt cgggcggcgc atccggcgcg tccctcacgg agagcagcgg
      481 cagcccgagg gtctcggacc aggcttccgc gagatcgcgt tcggacacca ggccgagccg
      541 cgccagcagc gccgtcaggt gcgtttccgg ggcttcggcg agcagccgct cggtgcgcgc
      601 caggtcatcg tgtttcaacc gtccgcgctg gaccagcagc gcgcatacgc gtgcttcggc
      661 atcgttcgcc ccggaacccg cgtgttccgt gctgggctgt gccaccatgg gacgttcccg
      721 caccgtcggc cgacaaggcg ccattggtag cacacccgcc cgggcaatgc gagcgccgga
      781 ccgaccccta ttgcgtcttc accaatcagg tgaccaaagg ctttttgcgc ggatgaacgc
      841 ggataaatgg tcttcaccgg tgttcatccg cgttgatccg cgtcggaatt catcttttcc
      901 actgccgacc acgccacgcc caggcccacg atgcccgtga atacggccgt ctgcggcagc
      961 gcctcgccgt cgcggaatcc acaacccggg tggggctcgc cgcagcgctc gccgccctac
     1021 atcgacgatg gcggcgagtc gatcgtgtag gacaggtgca aggtgatcgt gtcgcctttc
     1081 ttcaggtgct ggcgcgcttg cgggaagtgg accttcaggg tgccctcgtc ggtccggacc
     1141 ttcacgatgc cgtcctgact gaccgactcg accgtgccgc gcatgtcgtg ctgccccatc
     1201 tcctgccccg tctgggactg gggcgttcgt ggcggcatcg acgtttggtc ggggttctgc
     1261 tccggggtgt tgtcctgcgg aggcggcgtc tggaacggtg gtgtgtgggt gaccgcgccc
     1321 gactggggat gatcctcgct ggccggattg gtctgcgcga ggacgacagg tgccacgccc
     1381 gcaagcagcg cggcaatcgc gacgcccgtc cacgggtata tgcgatgcat gggaacctcc
     1441 ttgtgtgcct gaacggtcgc ggccggccgg gagagggccg gccgcgaacc accgctttgg
     1501 tggcgattcc actccatgcc ttcgctacga cttggtcaag tgactgcgcc gtggcgttca
     1561 gcggggctga acaatcgagc cagcgattat tcagcttcgc attgaagcga gcagcagatt
     1621 tgataaagcg accatcatca tctaggtacc gttacataat gacaggtcgg ctgatcgccc
     1681 ccgacactca cgcattcaag aaccgccgtg gtgctgcccg tcggttggat tccgtaatcc
     1741 tgcagcagtt cttgaaaatt gtgcgcatcg tagctcggat aggggttagt gaagtcgtag
     1801 acatctcgcc cgccgccggt gattgcctga taattatcag gaatcagatt tccatgccca
     1861 tcccgagatt gcccgaccac atagctcaat gtgccgtcct gggggttata gtggaaagtc
     1921 accttacttc catcgtgaaa cacgaacacc atcgtaagcg agaaatcgaa cgacaagacc
     1981 ccggaattga tgtcattgag taaagctgcc accgcagcag cgagcgcagg cgacacgtac
     2041 ggaacaccct tagtgtttga tgtcgcaaac agggtggtga agaaccagtt tttgacagca
     2101 tctttgtttt gggttgcatt caccacgtca taggcattca tgtagccatc atcaccgaga
     2161 ctttcgacgg gcctgagatt ggtgttgatg gatatttggg cggtctgggt catagagtgc
     2221 ccattgagcg catatacgtg ttgaaatgcc tcgaacgagt tctggtccct ggcagatggc
     2281 tgcgcataga aatattcctt ttgtccaccg gggttaccta ggtatggcgt agccgttggc
     2341 cggacgtttt gttttctgta acatcccaag tacaagtggt ccgaatcaaa atccccaaca
     2401 tactgatacc cttggtcgca gtttggcaaa agcgcctcta tctggggcgc ggtacatcca
     2461 ttacaggtct tgccatgctg aacgacttga gcgtgcaaag tcgcggaaaa gaacagtccc
     2521 actgcgaccg cgagtgcagt cgatcgatac gaatgcttca cgagtagcct cccttggcgt
     2581 ccctagccgg ggcatttttg accgtacgcc cgccctccgc cggtgtcaag ttcattcatc
     2641 tgagtcacaa gcttccgtca acactcgacc acgttgaccg cgagtccgcc gcgcgaggtt
     2701 tccttgtact tgtccttcat gtcgcggccg gtgtcgcgca tggtcctgat gaccttgtcg
     2761 agcgacacgt ggtgcttgcc gtcattcttc atcgccatcc gctgggcatt gatggctttc
     2821 accgaaccca ttgcgttgcg ttctatgcag gggatctgca ccaggccacc gatcggatcg
     2881 caggtgagcc cgaggttgtg ttccatgccg atctcggcgg cattctcgac ctggtggtgg
     2941 gtgccgccga gcgccgcgac cagcgcgccg gccgccatgg aacacgcgac gccgacctcg
     3001 ccctggcaac cgacctcggc gccgctgatg gaggcatttt ccttgtagag gatcccgatc
     3061 gcgccggcgg tgagcaggaa atcgatcacg ccctgctcgt tcgacttcgg gcagaagcga
     3121 tcgtagtagt gcaacacggc gggcacgatg ccggccgcgc cattggtcgg ggcggtgacc
     3181 acgcgtccgc cggcggcgtt ttcctcgttg acggcgagcg cgtagaggtt cacccagtcc
     3241 aacaccgtca acggatcctt gagcgccgct tccgggcgct ccttcaactc gtcgtacatg
     3301 gccggcgcgc ggcgcttcac gtgcaatccg cccggcagtt cgcccggcga gcgcaggccg
     3361 cgcgcgacgc agtcctgcat cgccttccag atctcgagca agcccgcacg gatctccgcc
     3421 tcgctgcgcc aggccttttc gttttccagc atcagttgcg cgatgcccat gccgtggcgg
     3481 tcgcacaagg ccatcaactc gtcacccgaa cggaacggaa acggcagcgg cgtggtgtcg
     3541 gcgacgatgc ggtcggccgc ggcttcgtcg gcgttgacca cgaagccgcc gccgaccgag
     3601 tagtagtcgc gactgacgat cgccttccct tccgcgtcga acgcgctgaa gcgcatgccg
     3661 ttcacgtgga acggcagctt ctgtcgcttg ttccagacga ggtcggtctt ctcgtcgaag
     3721 gcgatctcgt gggttgcacc gaggcgaacg cgcttgtccg agcggatgcg ttcgagcagc
     3781 ggcggtatcg cgtcgggatc gatgcggtcg ggccagttgc cttcgaggcc gcacagcacg
     3841 gccttgtcgg tgccatggcc gcgtcccgtc attgcgagcg agccatgcaa ttccaccgtc
     3901 acgcgagcga cgcgggcgag gtcgccggtg tcggccagcc aacgctcgac gaaacgcgcc
     3961 gccgcgcgca tcggcccgat cgtgtgcgag gacgagggcc cgatgccgac cttgaagatg
     4021 tcgaagacgc tgacggccat gggtgcgaag tggggacggc gagcccgtta ttctacgctg
     4081 cggccggtga aaagccgggg cgggcgatgg gatggaaggg tccagaatgt gcgcccgtat
     4141 cagctttcag gccaaagcca ccgcggcttc ccacgacttc tgccggtgcg ccccactacc
     4201 cgcccccgat ccccgctttc gaatctgtgc catgctcacc ctctaccaac gcgacgactg
     4261 caagctgtgc gacgaagccg tcgcgctgct ggcccgcgtg cgcgcaccgg aattccagag
     4321 cgtgtggatc gacggcgacg ccgggctcga ggcgcgctac ggtgtacgcg tgccggtgct
     4381 gcgcgacgaa gcgtcgggcc gggaactcgg ctggccgttc gacgcggagg cgttgcgcct
     4441 cttcctggac gcgtaggcgg gcatcgccgc cggcgtcggg caggctggca gtggctgcct
     4501 actggtccgg agtcgtcgtc gcgcgcaact gcaggtggcc gtgcacggtg acgtcgttgc
     4561 cgatcatcga cgggtcggcc cactgcccgg tgccgattcc gaagtcgagg cgtttcagtc
     4621 gcgccgtgac atccagcgtc gcggtgtcgc cgtgcggcgt gaacgtcacc gccaactgga
     4681 ccggcttgac gacaccccgc aggttcagtt caccgagggc gaccaccttg ccgtccgcac
     4741 ccttccggaa ggtggtggtc gcgaaatacg ccttcgggaa tttcgcggca tcgaagaagt
     4801 ccccaccgag cgccgtatgg tcgcgttctt cgttccgggt gtcgaggctg ccgatgtcga
     4861 cggcgacgtc gaatttcgcc ttcgcgaggt cgccgggatc gtaggcgatc ttcgcatcga
     4921 agcggcggaa gcgtccggtg tattcgacgt cctggtaagt gttggtgaac gtcaggctgc
     4981 tgtgcgccat gtcgacctgc cacgtcttcg ccatgccggg cgcggcaaag cccagcacca
     5041 acgccgtgca cattcctgcg agcagcaact tcatcgtccg cctccgtttc gtgggcgggg
     5101 gcccagtacc atccgccgca gcacctcgtc gcgctgccgg aagtggtgcc acaacgccgc
     5161 acctacgtgg gcaaccagta gcgcaacgag tatccagaat agcgtctggt gcgccagcag
     5221 cgcgcgcggc ttccacaccg ggtcgtagcc gcccgtgagg ctcgggatat cgaaaagccc
     5281 gaaccacacc agcggtgcgt tcgacgccga attgaaccac cacccggaca gcggaatcgc
     5341 gaggatgagc acgtacaggg cgaggtgcac cgcgcgtgcc gcgccgcgct gccagcgcgg
     5401 catcccggcc ggatcgtcgg gtcgccgttc gccgagccgc catgcgatgc gcagcaacac
     5461 cagcgcaagg atcgtcatgc cgatcgactt gtgcagggcg aacacgcgca ccttggcggg
     5521 cgtcagcccc atctggatca tgctgaggcc gatcaccgcc tgcgcgagga tcagcgccgc
     5581 gaccagccaa tggaatgccc gcaccagcgc gccccacccc gcttcagtgc tgcgcagcgg
     5641 catgcgcctt gctcctcgcc gccttctcgg ccgcctgccg gtagtcgaac tcggcgctgc
     5701 ggtccagctt ggcttcgatc tcgagccgga tcgcgacctc gatgccgacc gatttcggga
     5761 atgcggtcat cccgaacttc gtgcgatcga ggttcgcgct gcccgagaac cccgcgatcg
     5821 tctcgaatcc gaacagggtc acgccgaccc ggttcacggt gaaatccacc gtcaccggca
     5881 gcgtgacgcc gcgcagggtc agctcgccgt cgagggttcc gccgtgctcg ccggtcttgc
     5941 gcaccgcagt gctctggaaa tgcgccgagg gaaactgcgc ggcatccagg aaattgcgcc
     6001 cggtgaccgc ggcgttccag ccaccgtccc ccaggtccgc cgacgcgagg tcgatatcgg
     6061 cgacgacctt cgatgcgtcc cagtcgtcgg ggtcgaaccg cagccagccg cgcgcgatcg
     6121 caagtcttcc gaccggacgt gaatagccat cgtggtcggc actgaagagc acctgcgagt
     6181 gcaccgtgtc gatccggtag tcgagcgggt gcgccgcggc ctcgccggca gcgagcaaca
     6241 cgagccacaa cagcgaagtc aggcatcgac gcacagacac ctccgtttcg tcggacatac
     6301 tcgcatgcaa taggtgccgc gcgcatcccg gatggtttta cggcatcatc ggagaccggt
     6361 ccgcggggaa aggggacatg ctgactgcac tgatcctggc cggcgcattg gggaccgccg
     6421 ctcctgcagc ggcggcaggc gtgccgccta cgccgatttt ccgcagctat ggcgtggccg
     6481 atggcctgcc atccgccaac gcgtacgcga tcacccaaga tcacgcgggg ttcctctgga
     6541 tcggcaccca tgacggcctc gtgcgctacg acagccggga attccgggtg ttccgccacg
     6601 agcccgggca gcccgcatcg ctccccgcca acgacgtctc ggccctgctg gtcgaccggg
     6661 acgggcgact gtggggcggc ggcgaaggca ccggcctcaa ccgctacgag cccgcgacag
     6721 gcggcttccg gcactggctg cacgcggcgg accgccccgg cagcctctcc ggcaacgacg
     6781 tgatggcgat cgcccaggac gatgccggcg cgatctgggt cggcgtctac gcaggtggcc
     6841 tggacaagct ggacgccgac ggaaaaggct tcacccacct gcgccaccgc gaagacgacc
     6901 cgtcgagcat cgtttccaac aacgtcaccg cgctcgcgcc cgccgccgat ggcggcctgt
     6961 gggtcgggac cgacgcggga ctcgaccggc tcgatgcgaa tggccgcatc catcacgtca
     7021 ggcttccggg attctcgacc caacccacca tctggcaggt gcgggtggcc tcgacgggag
     7081 tcgatgccgc gaccagcgcc gggcttttcc acctcgacgg gaaaggcggc gccagcctgc
     7141 tcggcaccgc gcgcgaaaca ttcgccagcc tgcgcgaccc ggacggcagc ctgtggatcg
     7201 cgcaacgcgg cggcctcgcc cggatcgacg ccgacggcag cacgcgcttc gagcagccca
     7261 ccgagggcgt cacggggagc cttcccggcc gcctgcccga tggcttgttc cgcgaccatg
     7321 aaggcggact gtgggtggcg ctggtggacg gcggactggc ctatcttccg ccgcagtggc
     7381 gcgccttcag catgttccgc cacatccccg gggacgccgc tagcctcgcg cgcgaccgcg
     7441 tgcgcgcgct ggcgctggct ggcgatggca ccctctggat cggcggcgcc gccctgctcg
     7501 atcgcatcga cctcgccgat ggcaaggtgt cgcacactga cataccgggg ctggcccgca
     7561 tgtccatctc ggcgctcgcc gtggaccccg ccggacgtct gtggatcggc agcagccggg
     7621 ggttgttcct gcgggaccag ggacggcttc tgcccatcgc cgagggcgat cccggcctgc
     7681 gccacggcgt gtggcgactg ctggcagcgc gcgacggggc catctattgc gccggcgtgg
     7741 gcaccggcgt ttttcgcgtg gacctcggcg atcgtgcaat ccgtccggtc gcgccaccgg
     7801 gcaaaggcga gaacgcgcag gaaatcgtcc agcttcgcga agccggcgac ggggtgatct
     7861 gggcggccag caaggcgggg ctcgcgtggc tcgcgcccgg ggccgcgggc ttcgccttcg
     7921 tgcccgggat cgcccgcggc aaggtgctgg gcttcgcctt cgggcccggg gcgaccgtct
     7981 gggtcgcgcg tgacgatcgg ctgcagcaat accgactgga ccacgggcgt gcgaagctgg
     8041 tgtcggaaac cgggcacgac gaaggctggc ccgataccgg cgtcctcgcg ctcgaaacgg
     8101 gtccggacgg ccgcgtctat gcgctgggga cccgcaacct gctggtgtac gggccgcgcg
     8161 accaccagct ccacgcgttc accaccgccg acgggctcgc gaacaccgac ttcaccagca
     8221 gcgtgctgtt gcgtgccggc gacgaattgt ttgccggcgg gcccgcgggc ctggtcggcg
     8281 tgcgggtcgg cgaattgccg cagcatctcg cgacgccgaa ggtcgcgctg gagtcggtga
     8341 cggtgcatcg cgacggccgc gcgcagaagc tcgacccggc cggcgggatc gacctcgact
     8401 ggcgcgatcg cgagctcacg gtcacgacac aggtcctttc gttcgtggat ccggcacgca
     8461 accgctaccg gttccgcctg cagggcttcg accatggttg ggtggaaacc ggcagccgca
     8521 acgtgcgcga gttctcggtg ctggaacccg gcgactaccg gttgctggtc agcggccgca
     8581 tcggcgacgg cccgtggagc gcaccgttcg aggccttttc gctgcgcgtg gcggcggcac
     8641 cctggaccac gccctgggcg tgggcgctgt acgtgctcgc ggccgtggcg ctgctcgcat
     8701 ggttcgtgtg gatgttgcgc cggcgcctgc agcaacagca ccggttggcg ctgagcgagg
     8761 agcgccagct catcgccgag caggccaacg cggccaagtc gcgcttcctc gccaacatgg
     8821 cccatgagat ccgcaccccg ttgaccggcg tgctcggcat gaccgaactg ctcctgcaca
     8881 ccggactcga ccagcgccag catcgctacg cggatgccat ccggcgctcg ggcgcgttgc
     8941 tgcttcggca cgtcaacgat gccctcgacg tcgcgcgcat cgaggcgggc cggctggaac
     9001 tcggcgttgc gccgttcgat ccggcggcgg tcctgcgcga agtggccgaa gccgatgcgg
     9061 gactcgcggc gcagaagggg cttcgcatcg acgtcgagat cgcgccggat gccccttcgg
     9121 cggtacgcgg cgatgcccag cgggttcagc aggtcctgtt caacctggcg cacaacgcgc
     9181 tgaaattcac ggcccacggc ggcatccggt tgaagctgga gcgtgtggac gacgacatcg
     9241 tgtacagcgt gatcgaccgc ggccgcggca tgacgcccga ggaatgcgcg cgggtcttcc
     9301 agcgcttcga gcaggcggac caggggcggc tgcagcgcgg cagcggcctc ggcctcgcga
     9361 tcagccgcga gctggtcaat ctgatgggcg ggcgcatcgg cgtgcagtcg gtccccggct
     9421 acggcagcac cttcagcgtg cgactgccgc tgccgatcgt ggcggtggcg cagggcgatc
     9481 gtgcggcttc ggccgccggc gccacgtcgg cggcacaccg cgacacgcag ccggttgcga
     9541 caagagaggc tgcggaaagc acgacttcgc cgggacatcg cgtattgttg gtcgaggacg
     9601 atccggtgac gggcgaagcg ctcgccggcc tgatcgaggc gttcggacac caggtgacgc
     9661 tcgccccgca ggcgctggcg gcgctttcgg aaatcgacgc cggcggcagc ttcgacgcga
     9721 tggtgttcga cttcgacctg ccggggatgg acggctgcga actcgcggcg ctgttgcgcc
     9781 agcgcggtgt ggccacgccc atcgtcgcac tcaccgccag tgcgcacggc gacgaggaag
     9841 aacgcgcgcg cgcggcgggc atggacgcgt tcctgcgcaa gccggtgctg cctggtccgc
     9901 tgcacgaggc cctggacgcg gtgttggccg agacctccgt tgccggcgag cggccgaata
     9961 cgttgttgta ggagcgcgcc atgcgcgaag cgcttgctcc ttcttttgcg cgcgacaaac
    10021 ggttaggaca agccgggcgg ttgtcgcggc cagcggccgc tcctacgggg tagcggatga
    10081 tccctgatgc accgcgggtt tgtacgtggc gtacttcgcc agcagcgcgc ggaaacccgg
    10141 gtcgcgccgg atcggatccc aggtcgggtc gagccacagc agcaggggcg aatagacata
    10201 gctgatgccg cgtttcgcca gggctttcgc cagcagcggc accgcgcgat ccgggcgccg
    10261 ggcctccgcg tacaaggcgg catcgcgctt catcatcacc gggccgttgg tctggtcctt
    10321 cgtcgcggcg acgatcgcct gcgccctccc gatcgcatcg agtcctgccg cggtcctgcc
    10381 gaggccgagt tcggcgctcg cgacgtgttg ccaggcgccg gcgagcttca ggttttgctg
    10441 ccgctcgagc tgcgtccgcg ccgtcacgag cgccctgctg aacaacggac gcgccctggc
    10501 cgtgtcgccc gaaaggcggt agaggtcggc cagcagcagc gccttcgatc cgatgccgac
    10561 cgaaaagacg tcggggctgt cgggaacgct ctccagcagc gcgatcgcgt cgtggtcctt
    10621 gcgctgcatg ctcagcagcg acacccgctg gatttcgagc gcaggcgcat cgccggcgac
    10681 cgcggcgagc gccttcgcga tgtcgccgct gtcgagcagg atggcctgcg agcgccacac
    10741 cttggcgttg agattggtgg gatcgagtgc cagcgcgcgc tcgaaccacg gctcggcgtc
    10801 ggcatagcgc gcggccatca tgcaggtgta gccgatttcg taggcgagcg agccattgcg
    10861 cgggtcgtgc gccagcgccc ccgtgagcga cgtgatcgcg tcgccgaagc gcccctgccg
    10921 gcgttcgacg tagccgcgcg cggccagcgc atcggcatcg ttcggccgca ccgcgagcgc
    10981 ggcatcgaat gcgtccagcg cgccgtcgta gtcgcgggcg ccataatagt cgctgtagcc
    11041 catcgcgatc cgcgaggccg cgaaatccgg cgcgagtttc agcgcctgct tcgcatcggt
    11101 gcttgcctgc gcggtgagtt gccccggatc ctcgccgccg ccgccgaacc agccgaggat
    11161 gctctcgacg taggacagcc gggcatacgc gagggcgaag cccgggtcgc gcgcgatcgc
    11221 ctgccggtac agcgggatcg cggctttcca gctcgcggtg tcgtagttcg tgtcgccgcg
    11281 gttggcgtcg tagtccgcgc gcaggaacaa gtcgtaggcg gcctggtcgg tggtcggcac
    11341 cgcgtcgagg cgcgcggttt ccgcgggact gagtttcgca tccagcgtca ccgcgatctt
    11401 cccggcgacc tcgccctcga ctccgaaaat gtcggcgagc ttgcgctggt aatcctgcgc
    11461 ccacacgtgc gcgtcggttt ttgcatcgat caactgcacg ttgatcagca cttcgtcgcc
    11521 ggccttctgc acgctgcctt cgaggatcgt ggccaccccg agttgccggc cgatggcctc
    11581 caggtcgtcg ggacggctct ggtatttttc ggtcgaggtg cgcgcgatga ccttgagatc
    11641 gccgatgtcg gcgagcttgg tcaggatcag gtcctgcatg ccggcgacga aataggcgtt
    11701 gtccttgtcg ttcgagaggt tctcgaatgg caacaccgcg atcgatttgg cggggatcgg
    11761 ctgggccgcg cggaccggcg tcgtgcgcag tccgggcgca gccgcgccgg cggcggaagt
    11821 cggaacggcg gatgccggat tccgctgcgc tgcatccggg ccacgggcag ccgctaccgc
    11881 attccccggt gcggtcgccg gcgatccgct gcgcccgaag tgccacagga ggccgccgcc
    11941 gatcgcgagc accagcgcga tcagcagcaa ttccggaccc gagatccggt cttcgccgcg
    12001 ctcgccgtga taccacgcga gcaccagcgc gatcgcgaaa cccaaggcca gcacgccgaa
    12061 cgcggcgtgc atcaccgcgt gcggccaggc atagctgtcc gccacgactt cgagcacctg
    12121 gatcagcgcg aacgcggcgg cgacgtacgc a
//
LOCUS       DASSNH010000112         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_797645, whole genome shotgun sequence.
ACCESSION   DASSNH010000112 DASSNH010000000
VERSION     DASSNH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797645"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..519)
                     /locus_tag="VFK00_03965"
     CDS             complement(232..519)
                     /locus_tag="VFK00_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592144.1"
                     /translation="MEPRRWLPYQRLQSRHLALALAAARVIAWIGMLLIAAGIAYGIA
                     VGVKGTAAPAPLTLGTVLGLIGYGLAALVVSGALAALVGIEESQRRQAERE"
     gene            complement(708..>3012)
                     /locus_tag="VFK00_03970"
     CDS             complement(708..>3012)
                     /locus_tag="VFK00_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET7592145.1"
                     /translation="KQTAPVYYITGTIHSTETGAPTALMELAYRLAVDDAPYIQYIRS
                     HMITLITPVVEVDGRDRMVDLYRWHRAHPGENYPHLLYWGHYVAHDNNRDAMGVTLDL
                     TRNVLNTYVDWHAQVLHDLHESVPFLYDNTVGDGPYNAWIDPILTSEWQQIGWNNVEQ
                     LTKLGLPGVFTHGDFDTWSPGYLMFIAAMHNGISRLYETFGNGGADTEERILEPDEYA
                     RTWYKPNPPLPKVLWSQRDNNNYEQSGLLTALYYFAQHREHFLENFWLKSKRSVEKPQ
                     LAGPAAYVLTADERDKGAQMHLLHALHLQHVEISRAGAPFTVKVAGDTGSNDGGKAKG
                     KSSGKSSARAEPPRVASVKRTFPAGSWIIRMDQPYSRIADALLDRQYWSPDDPQKHPY
                     DDTGWSFGDLFGVDVARVVDTSVLKVPMKEVQGDFDQPAFTLASLGVTERGRMPRIAL
                     MHTWLSTQTEGWWRMALDKLHVPYTYISTQDVSREPDLRGKYDVILFGPVGYASSQMI
                     IDGLPMYGNPMPWEKTELTPNLGVIDSTPDVRPGLGQTGVANLKRFVAEGGLLVASED
                     TARFAIDIGLAPGVSVAKAGDLRVVGSVLRAELVDKASPVAAEYDDSFALYSSEGMSF
                     NVADQTIGNRGLSTARDYQRPTGRGGPDDADIPEGRPYQAPPELPDVKPWQAVPLNAE
                     QARNNPFLIPGADRPKVIARWADAGDLLISGLLENGDGMAGRAAVVDARYGKGHVLLF
                     ANNPVWRGETIGSYRMFFNAITHYDAL"
BASE COUNT          501 a          963 c          992 g          556 t
ORIGIN      
        1 cgacataact gactccttac tactcgattg gttgagaaac agcgcacttg cgtgcgcaac
       61 ttgaagcact gactagcgag agtgaagaca gcgatgcgat cgttgacacg gtcagcaacc
      121 ggccccaaac aaaccgcccc cgacggtttg gctaactcag cgggcgcgat cctacagcac
      181 tttcataccg aaatgccagt gcttatgccc aataatcgtt tttagccgga atcattcgcg
      241 ttctgcctgc cgccgctggc tttcctcgat cccgaccagg gccgccaagg ccccactgac
      301 caccagcgcg gcgaggccat agccgatcag gccaaggacg gttcccaagg tcagcggcgc
      361 cggagccgcc gtccccttga cgcccacggc gatcccgtag gcgatcccgg ccgcgatcag
      421 caacatgccg atccaggcaa tcacccgggc ggcggccagc gcaagcgcca gatggcggct
      481 ttgcagcctt tggtagggca gccagcggcg tggttccatg aggggatttt cctattccag
      541 ggccgcatcc ggagcggctg ttgcgaacag tcaggccatg gatggccctt tggcattcgt
      601 gggatgccgc catcagggat gatcgcaaag cgcggtcgta gggcggacat cgtccgccaa
      661 ttcccaggcc aagggcggca atgtcgccct acctggcgcc cgcagcccta caacgcatcg
      721 taatgcgtga tggcgttgaa gaacatccgg tagctgccga tggtttcgcc acgccagacc
      781 gggttgttgg cgaacagcag cacgtggccc ttgccgtagc gggcgtcgac caccgccgcc
      841 cggccggcca tgccgtcgcc gttttccagc aggccgctga tcagcaggtc gccggcgtcc
      901 gcccagcgcg cgatcacctt cggccgatcg gccccgggga tcaggaacgg attgttgcgg
      961 gcctgttcgg cgttcaacgg cacggcctgc cacggcttca cgtccggcag ttcgggcggc
     1021 gcctggtatg gccggccttc gggtatgtcg gcatcgtccg ggccgccgcg gccggtggga
     1081 cgctggtagt ccctggcggt ggaaaggcca cggttgccaa tggtctggtc ggccacgttg
     1141 aaggacatgc cttcggaact gtagagcgcg aaggaatcgt cgtattccgc ggcgacgggg
     1201 ctcgccttgt cgaccagctc cgcgcgcaaa acgctgccga ccacgcgcag gtcaccggcc
     1261 ttggcgaccg aaacgcctgg cgcgagaccg atgtcgatcg cgaagcgcgc agtgtcttcc
     1321 gaggccacca gcaggccgcc ttcggccacg aaacgcttga ggttcgcgac accggtctgg
     1381 ccgaggcccg gccggacatc gggggtcgaa tcgatcaccc ctaggttcgg cgtgagttcg
     1441 gtcttttccc acggcatcgg gttgccgtac atgggcaggc catcgatgat catctgcgag
     1501 ctggcgtagc cgaccggccc gaacaggatc acgtcgtact tgccgcgcag gtccggctcg
     1561 cgcgacacat cctgggtgct gatgtaggtg tagggcacgt gcagcttgtc gagcgccatc
     1621 cgccaccagc cctcggtctg cgtcgaaagc caggtgtgca tcagcgcgat ccgcggcatc
     1681 cgcccgcgtt cggtgacgcc cagggacgcc agcgtgaatg ccggctggtc gaaatcaccc
     1741 tgcacttcct tcatcggcac cttcagcacg gaggtgtcga cgacccgtgc gacgtccacg
     1801 ccgaacaagt cgccaaagga ccagccggtg tcgtcgtagg ggtgcttctg cggatcgtcg
     1861 ggcgaccagt actgccgatc cagcaaggca tcggcgatcc gcgagtacgg ctggtccatc
     1921 cggatgatcc agctccccgc cgggaacgtg cgcttcaccg aggcgaccct cgggggctcc
     1981 gcccgcgcgg atgacttgcc gctcgacttg ccttttgcct tgccgccgtc gttgctcccg
     2041 gtatcgccgg cgaccttgac cgtgaacggt gcgccggcgc ggctgatctc gacatgctgc
     2101 aggtgcagag cgtgcagcag gtgcatctgc gcccccttgt cccgctcgtc ggcggtgagg
     2161 acgtaagcgg cgggtcccgc gagctgcggc ttctcgaccg aacgtttgga cttcagccag
     2221 aagttctcga ggaaatgttc gcggtgctgg gcgaagtagt aaagcgccgt cagcaggccc
     2281 gactgctcgt agttgttgtt gtcgcgctgc gaccacagca ccttgggcag cggcggattc
     2341 ggcttgtacc aggtgcgcgc gtattcgtcg ggctcgagga tccgttcctc ggtgtcggcg
     2401 ccgccgttgc cgaaggtttc gtacagacgg ctgatgccgt tgtgcatcgc cgcgatgaac
     2461 atcaggtacc cggggctcca ggtgtcgaag tcgccgtggg tgaagacccc gggcaggccc
     2521 agcttggtca actgctcgac gttgttccag ccgatctgct gccattcgct ggtgaggatc
     2581 gggtcgatcc acgcgttgta gggcccatcg ccgaccgtgt tgtcgtagag gaacggcacc
     2641 gactcgtgca ggtcgtgcag gacctgcgca tgccagtcga cataggtgtt caaaacgttc
     2701 ctcgtgaggt cgagtgtcac gcccatcgcg tcgcgattgt tgtcgtgggc gacgtaatgg
     2761 ccccagtaaa ggaggtgcgg gtagttctct ccgggatgcg cacggtgcca tctgtacaga
     2821 tcgaccatcc ggtcgcggcc gtccacttcg accaccggcg tgatcagggt gatcatgtgc
     2881 gagcggatgt actggatgta cggcgcgtcg tcgacggcga ggcggtacgc gagctccatc
     2941 agcgcggtgg gcgcgccggt ctcggttgaa tggatcgtcc cggtgatgta atagaccgga
     3001 gcggtctgct tg
//
LOCUS       DASSNH010000113        13005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_511007, whole genome shotgun sequence.
ACCESSION   DASSNH010000113 DASSNH010000000
VERSION     DASSNH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 13005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13005
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511007"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFK00_03975"
     CDS             complement(<1..476)
                     /locus_tag="VFK00_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HET7592146.1"
                     /translation="MDRHLRRVTPHVVRRQAIRRKTRHCHPHFYARCAHWSFHAEAPA
                     IAFRWARERWVLGGGAFLLVLLTALGIPAWASVIRHKAVQPVTKETLALQLPPAPKEA
                     PRAAVASWKSVEVQPGETLSEIFQEQGLSSADLASVMASGKDTGALKTLHPGDQLE"
     gene            673..1887
                     /gene="tyrS"
                     /locus_tag="VFK00_03980"
     CDS             673..1887
                     /gene="tyrS"
                     /locus_tag="VFK00_03980"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7592147.1"
                     /translation="MTDHQPSLALIHRGAVDVLKDDELEARLALDRPLRIKAGFDPTA
                     PDLHLGHTVLLNKMRQFQDLGHTAIFLIGDFTGMIGDPTGKNVTRKPLTPGEVQANAD
                     TYARQVFKVLDRERTELRFNSEWFGKMTAADMVKLAAQHTVARMLERDDFSKRFKSEQ
                     PIAIHEFLYPLVQGYDSVALQCDVELGGTDQRFNLLMGRGLQEHFGQPPQIVLTMPLL
                     EGIDGVQKMSKSLGNYIGIDEPAIDMVTKTMKIGDELMWRWFELLSFEVSLDELASLK
                     RDVAGGALNPRDAKLRLARELAARFHGAAEAEKAIAGWHAVVRGEGDTSSLPLNEIAV
                     SVDGLRLPALLTAAGLTSSNSEANRKLKERAVRIDAVVADDPQRTFGPGFEGVLQVGK
                     RNFARVRLRAAG"
     gene            complement(1954..2631)
                     /locus_tag="VFK00_03985"
     CDS             complement(1954..2631)
                     /locus_tag="VFK00_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592148.1"
                     /translation="MNIRLHTSLAMALIGLSTIAATHPAKACGVNLVRSANGGWTIAP
                     PATMPASAARMMYLATAQQPTTKTIPNPLQSVERIAGLYEITMTAEGNGPTGLPDGTP
                     VDHAYVSLLADGNEIMNSGRPAGDGDFCMGTWAQTGVRTYMVNHFMLAWAQTVDADGT
                     QIDNTFVGPGHLQETITLSRDGSSYTGTFVLTQYDPNYNIITPGGIPIQGTVTGTRKT
                     IDSPVTY"
     gene            2790..5309
                     /locus_tag="VFK00_03990"
     CDS             2790..5309
                     /locus_tag="VFK00_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7592149.1"
                     /translation="MESAEHTEHVADASTFAFGDVVIDAGAHRLLRAGKEIAVEPKAF
                     SVLLEFLAHPGQLLSRDQLLDAVWGHSFVTPATLNRLIVQLRRALADDAEHPRCIQTV
                     HGLGYRFIAPLEPLKQKPVPTLRFAPPARARVPERTEPLIGRDADIETLKRMLREHRL
                     VTLTGPGGLGKTQAALETARAIATDFPDGVWLFDCTPQTDGDALARSMADMFEIHATA
                     DAAQLIARLGELLQTRRVLLVFDNCERVAEPLGKVVANLLSACADLRVLVTSQQRLNC
                     AGESLYPLPPLELPPECEWTTDEQIASLSSIPAVRLLLLRSQACASGFTLMRSNAATT
                     AEVCRRVAGLPLALELAAARLRLLSPEQLLARMDARLLNLAESNPGRPPRHQTLRALI
                     EWSFALLSEKEQSLLCGLSIFVGGCTLDGATAIGAALELEDPQTLELLGGLIDKSLLT
                     VDAATNPPSYRLLDSVRLFALRQLASSGNEVRVRTAHLEHFVQLTERIYTEIRSSRFQ
                     PWFERVRREWNNLLAAFDFALGQAGLADDALAIVANLCWYFRARSDYVQSAQLLDSAL
                     RAASQQASHRALALIALGMVSHQSQMHERAASCLHEGIALAADRHDPWLAAAGRAILA
                     FELANCGDFERAAEAVDSALAVASSLDDRWLRSQALLSRGIVYGLQGRHREGEAWLGE
                     AAECVTSPPNDAFQHFYALINRALLRHYLGSESQAARDWLDVLDGCVMLQNRRGASGC
                     VEGAAYLTIQRGEPAKAVRFLASAARVRELTDAPLMPLWRKGQEATEREARDMLGGAF
                     EVAWREGASARFEDIIGEARALLAEITGQPGRSGASNPSGS"
     gene            complement(5267..6652)
                     /locus_tag="VFK00_03995"
     CDS             complement(5267..6652)
                     /locus_tag="VFK00_03995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7592150.1"
                     /translation="MVSHSSRTQNFPWRDLQGPLRGTLLLPVDAGYDEARRVWNGAID
                     RRPLAIACCRDAEDVGAAVKFALRERVPMTVRGGGHNVAGLAVRDDALMLDLRSLNRV
                     EVDPRSRTARVEGGALWRDVDAASQPHGLATTGGFVSTTGVGGYTLGGGVGWLMRRCG
                     LAIDNLLKAEVVLADGSIVTASEDQHADLFWGLRGGGAGLGVVTRFAFRLHPVSEVHA
                     GIVFYPVEAGPALLRAFRVFTPDAADALTVMLVFTTAPPLPFLPPEAHGRRAVALAYC
                     WNGDPGHGAQAAASIARAAEPLGRHEGMMPYVAWQQMFDPAAPAGHHYYWTTSLFDAF
                     DDGLIEALVPRAVQPADALCEVHVHHLGGAAARVARDATAFSHRAAPFFINVIGHTDA
                     AERFTAIRDWVCDLRAAFAAYSRTGMQPNFAGEPADLKAQAHDAATQSRLQGLRAHYD
                     PEGLLAPLRPG"
     gene            6805..7485
                     /locus_tag="VFK00_04000"
     CDS             6805..7485
                     /locus_tag="VFK00_04000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7592151.1"
                     /translation="MFIDVPSRRRAHPRWVTPFLVVTCVACYLWLMLSSPATREVVLG
                     DWGTVPAHLFRRGVFLEGWLWLRPLTSLFMHADWLHLVGNMLFLVIFGLPAERALGSR
                     RFLALFLVGGAFANLVGAWTLSAQLRPIVGCSGAVSAILGAYLALFPRAHLGLVLPLG
                     LYLQFIRVPAALLIGMWVLLQLLFTWAGPGFGTVVWWAHIAGFGFGILFAVVSRGAVE
                     RRLRERMY"
     gene            complement(7482..8222)
                     /locus_tag="VFK00_04005"
     CDS             complement(7482..8222)
                     /locus_tag="VFK00_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET7592152.1"
                     /translation="MPHLLLVQTGEAPESIHARFGGFADWFRDAMRVPPAQLRVVRVD
                     EGAALPAPDSVAGAVITGSAAMVTERADWSERTAAWIRDAMDADTPLFGVCYGHQLMA
                     HALGGTVGWLPAGREMGTRIMTRHFRDGGDWLDDLPETFPAHATHRQAVLEPPRGAAV
                     LVSSNLDPHQLLRYAPHAVSTQFHPEFSAAAMRAYIDARAAVLRDEGVDVDALRGDVR
                     ETTAARALLERFAQAALSRRRDEEQALL"
     gene            complement(8272..10071)
                     /locus_tag="VFK00_04010"
     CDS             complement(8272..10071)
                     /locus_tag="VFK00_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET7592153.1"
                     /translation="MAAIPNSTATLGRNGRLELGEVLAALVADGLLAAEDAKRIRGSS
                     RAGRGAVELHPLVVISSGKPENRADRGKPLTVESLTVWLADKAGLPYLKIDPMKIDAA
                     AVTQAVSQAYAQRHRILPVAVNHGTVTFASAEPFDDHWAADLSQMLRREVKRVVANPL
                     DIHRYLMEFYGVQRSIAKAREAKQDAMDGSAILNFEQLLELGKAGEIGADDRHVVHIV
                     DWLLQYAFEQRASDIHLEPRREAALMRFRIDGVMHKVFELPPAVMTAVTARIKILARL
                     DVAEKRRPQDGRIKTRSAGGREVELRISSMPTAFGEKIVMRIFDPDIVVKQFSQLGFS
                     GEEEKTWREMVERPHGIVLVTGPTGSGKTTTLYSTLKHLARPELNVCTIEDPIEMVAP
                     EFNQSQVQPAIDFDFAAGVRTLLRQDPDIIMVGEVRDLETAQMAVQASLTGHLVLSTL
                     HTNDAPSAITRLLDLGVPHYLIQSTLSGVVAQRLVRTLCPHCKRKAKQDPQTWSVLTR
                     GWVIPAPAEVYEPVGCLECRKTGFLGRTGVYEMMPVTPRLRGMITAQLDLDGFTRFAL
                     EDGLQPLRISAATQVRRGLTTVEEVLSVLPAAG"
     gene            10167..11057
                     /gene="glyQ"
                     /locus_tag="VFK00_04015"
     CDS             10167..11057
                     /gene="glyQ"
                     /locus_tag="VFK00_04015"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7592154.1"
                     /translation="MAGPTFQQVIQTLNRYWAEQGCVLLQPLDLEVGAGTFHPATFLR
                     ALGPEPWAATYVQPCRRPTDGRYGENPNRLQQYYQYQVVLKPNPDDVLERYFGSLREL
                     GIDPLVHDLRLVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQAGGLECKPVTAEI
                     TYGLERLAMYLQNVDSVFDLVWTEAPHGIVTYGDVFRRNEVEQSAYNFEHADTTELFH
                     RFDACEAEANKLVALHLPLPAYEQVMKASHAFNLLDARRAISVTERQRYILRVRTLAQ
                     AVAKEWVAPREATNAEAAHG"
     gene            11050..>13005
                     /gene="glyS"
                     /locus_tag="VFK00_04020"
     CDS             11050..>13005
                     /gene="glyS"
                     /locus_tag="VFK00_04020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7592155.1"
                     /translation="MAERSLLVEIGVEELPIHAVDDLAQAFARSMCEGLVKRGIAADV
                     EHARAFATPRRLAVWVPDVAFTQPEQRSESFGPYVNIALDAEGQPTRALLGFANKAGV
                     EWTQLERGTDAKGERFVHRRVSPGRTTAELVPEIAGEAAAALPVAKPMRWGDHESSFV
                     RPVHWLVMLHGDQVIPGELLGLKAGRQSRGHRFMHPHPVHLADADSYADSLRAANVLA
                     DPAERRQRIRDEVARVAPAGCTPRLDEPLLDELANLSEWPAAIACTFDREYLDVPHEA
                     LVTTMRTNQKFVPVFDAEGRLTEHFIGIANLESKDPAQVRHGYERVIRPRFADAKYFF
                     DEDLKTPLGSYRKLLEDVVWQRALGSLWDKTVRVAELASAIAARLFAQGIDVDAAQAA
                     HAAGLSRCDLLTRMVGEFPELQGVMGRHYAAAQGERADVADALDEFYRPRNAGDGIAQ
                     GTLAQCLSLADKLDTLAGIFAVGMKPTGNKDPFALRRAALGLARTLIEGGLDVDVEAL
                     LPEHAALIRADMRKAAIALKRDQSGGRVAHLEAGDVVTLRIAEVYPFILDRLRGYYTE
                     QGFTPQQFEAVAAVRPASLLDFDRRLRAVAQFARQPEAEQLAAANKRVANILRKEGID
                     AEAAARHELDAELLCEPAGQALAQALDE"
BASE COUNT         2129 a         4258 c         4402 g         2216 t
ORIGIN      
        1 aactcgagct ggtcgcccgg gtgaagggtc ttgagcgcgc cggtatcctt gccgctggcc
       61 atgacgctgg cgaggtcggc ggaagacagt ccctgctcct ggaaaatctc cgacaaggtt
      121 tcgccgggct ggacctccac gcttttccag gacgcgacgg cagcgcgcgg cgcttccttg
      181 ggcgccggcg gcaattgcaa tgccagcgtt tccttggtca ccggttgcac cgccttgtgg
      241 cggatcacgc tggcccaagc cggaatgccc agcgccgtca gcaacacgag caaaaaggcg
      301 ccgccgccga gcacccagcg ttcccgcgcc cagcggaacg cgatcgccgg cgcttcggca
      361 tggaacgacc aatgcgcgca gcgtgcatag aagtggggat ggcaatgacg ggtcttgcgg
      421 cggatcgcct ggcggcgcac gacatggggg gtaacacgac ggagatggcg atccatcgga
      481 gtgtccggaa gcgggcggag aggcggaacg ggtcgtacca tagccgcctt gtgaaggacg
      541 cgtcaatgcc ttgtttcaca aagatttgca cgatctttcg tgattaacac ataattaacc
      601 ccggggtcac tttcgacgtt tgaacccgtc ccggtttttc ggccgacgga acccctttca
      661 gcaggactgt tcatgaccga tcaccaacca tcgctggcct tgatccaccg cggcgcggtc
      721 gacgtgctga aggacgacga gctcgaggca cgccttgccc tcgatcgtcc gctgcggatc
      781 aaggccggct tcgatccgac ggcgccggat ctgcatctcg ggcatacggt cctgctcaac
      841 aagatgcgcc agttccagga cctcgggcac accgcgatct tcctgatcgg cgacttcacc
      901 gggatgatcg gcgatccgac cggcaagaac gtcacccgca aaccgctgac gcccggggag
      961 gtgcaggcga acgccgacac ttatgcgagg caggtgttca aggtcctcga tcgcgagcgt
     1021 accgagcttc gcttcaactc cgaatggttc ggcaagatga cggcggccga catggtcaag
     1081 ctggccgcgc agcacaccgt ggcgcggatg ctcgagcgcg acgatttctc gaagcgcttc
     1141 aagtcggagc agccgatcgc gatccacgag ttcctgtatc cgctggtgca gggctacgac
     1201 tcggtggcgc tgcaatgcga cgtcgagctc ggcggcaccg accagcgctt caacctgctg
     1261 atggggcgtg gcctgcagga acacttcggc cagccgccgc agatcgtgct gacgatgccg
     1321 cttctcgaag gcatcgacgg cgtgcagaag atgtcgaagt cgctcggcaa ttacatcggc
     1381 atcgacgaac ccgcgatcga catggtcacc aagaccatga agatcggcga cgagctgatg
     1441 tggcgctggt tcgagttgct gagcttcgag gtctcgctgg acgaactggc gtcgctgaag
     1501 cgcgacgtcg ccggcggcgc gttgaatccg cgcgacgcca agctgcgcct ggcgcgcgag
     1561 ctggccgcgc gcttccacgg cgccgccgaa gccgagaagg ccatcgccgg ctggcacgcg
     1621 gtcgtgcgcg gcgaaggcga cacctcgtcc ctgcccttga acgaaatcgc ggtgtccgtc
     1681 gacggcctgc ggctgcccgc gttgctcacc gcggccggcc tgacgtccag caattcggaa
     1741 gccaaccgca agttgaagga acgcgccgtg cgcatcgacg cggtggtggc cgacgacccg
     1801 cagcgcacgt tcggaccggg ctttgaaggc gtgctgcagg tcggcaagcg caatttcgcg
     1861 cgcgtccggc tgcgcgccgc cggatgatcc ggacggacga ggcgcggcag gccgccgcgc
     1921 ctcagggtcc tctttcgctt cgcgctgtcg tgatcagtac gtgaccggtg aatcgatggt
     1981 cttgcgcgtt cccgtcacgg ttccctggat ggggatgccg ccaggagtga tgatgttgta
     2041 gttagggtcg tactgggtga gcacgaacgt gccggtgtac gaactgccat ccctgctcaa
     2101 cgtgatggtt tcctgcaggt ggcccgggcc gacgaatgtg ttgtcgatct gggtgccgtc
     2161 ggcgtccacg gtttgcgccc aggccagcat gaagtggttg accatgtagg tccgcacgcc
     2221 ggtttgtgcc catgtgccca tgcagaagtc cccatcgcct gccggccttc ccgagttcat
     2281 gatttcattg ccgtccgcga gcaaggacac gtaggcgtga tcgaccgggg tgccatccgg
     2341 cagccccgtc ggaccgttgc cttcggcggt catggtgatc tcgtacaggc cggcgatccg
     2401 ctcgacggat tgcaggggat tcgggatggt cttcgtcgtt ggctgctgcg ctgtggccag
     2461 gtacatcatc ctggcggctg aagcgggcat cgttgccggt ggagcgatcg tccacccgcc
     2521 attggcggac ctgaccaggt tcacgccgca ggccttggcc gggtgggttg cggcgatcgt
     2581 cgacagcccg atcaaggcca tcgcgaggga tgtgtgcaaa cggatgttca tggcgctctc
     2641 ctctggggct tgtccggcgg ccggcggcat gccccgctga ccgtcggtat aaaatgcacc
     2701 ctggaaggac ccgagtcatg atgtttcgat agggaaatca cgagagtgcg tagtggcgtt
     2761 gacgtggtcc ggacggagcg cgcagcggca tggaaagtgc ggaacacacg gagcacgtgg
     2821 cggacgcgag tacgttcgcc ttcggggacg tagtcatcga tgcaggtgcg caccggttgc
     2881 tgcgcgctgg caaggaaatc gcggtcgaac cgaaggcatt ctcggtcctg ctggaattcc
     2941 tcgcgcatcc tggccaattg ctcagccgcg accaattgct ggacgcggtg tggggccaca
     3001 gcttcgtcac gcccgcaacc ctcaaccggt tgatcgtgca attgcgcagg gcgctggccg
     3061 acgacgccga acatccacgg tgcatccaga ccgtgcatgg cctcggctat cgcttcatcg
     3121 caccgctgga gcccctcaag cagaaaccgg ttccgacgct ccggtttgcg ccgccggcgc
     3181 gtgcacgggt gcccgagcgc accgagccgc tgattggacg cgacgccgac atcgagacgc
     3241 tgaagcggat gttgcgcgaa catcggctcg tcaccctcac cggcccaggt ggcctcggca
     3301 agacgcaggc ggcgcttgaa accgcgcgag cgattgccac ggattttccc gacggcgtgt
     3361 ggctgttcga ttgcacgcca cagaccgacg gcgacgcatt ggcgcggtca atggcggaca
     3421 tgttcgaaat ccatgcaacg gcagacgcag cgcaacttat cgcccggctg ggcgaactct
     3481 tgcagacacg ccgcgtgttg ctggtgttcg acaattgcga acgcgttgcc gagccgctgg
     3541 gcaaggtggt cgcgaacctg ctttcggcct gcgcggacct gcgtgtcctg gtaaccagtc
     3601 agcagcgcct gaactgcgcg ggagaatcgc tgtatccgct gccgcccctg gagttgccgc
     3661 cggagtgcga atggaccacg gatgagcaaa ttgcttcgtt gtcgtcgatc cccgcagtac
     3721 gattgctgtt gcttcgatcg caggcctgcg cctccggttt cacgctgatg cgcagcaacg
     3781 ccgccacgac cgcggaggtc tgccgccgcg tcgccggctt gccgctggcg ctcgagttgg
     3841 ccgcggcacg cctgcgcctg ttgagcccgg aacagttgct ggcgcgcatg gatgcccggc
     3901 tgctcaacct ggccgagtcc aaccccggcc gccctccgcg ccaccagacg ctgcgtgcct
     3961 tgatcgagtg gagcttcgcg ctgctctcgg agaaggagca atcgttgctg tgcgggttga
     4021 gcatcttcgt gggcggttgc acgctcgacg gcgccaccgc gatcggcgcg gcattggaac
     4081 tcgaggatcc gcagaccctg gagttgctgg gcggcctgat cgacaagtcg ctgctcaccg
     4141 tcgatgccgc taccaatccg ccttcctacc gcttgctcga cagcgtacgg ttgtttgcac
     4201 tgcgccagct ggcttccagc ggaaacgaag tacgtgtgcg caccgcgcac ctcgagcatt
     4261 tcgtgcagct caccgagcgc atctacacgg aaatccgcag cagtcgtttc cagccgtggt
     4321 tcgaacgcgt aaggcgcgaa tggaacaacc tgctcgcggc gttcgatttt gcacttggcc
     4381 aagccggtct agccgacgac gcgctggcga tcgtcgccaa cctctgctgg tacttccgtg
     4441 cgcgctccga ttacgtgcaa tccgcgcaat tgctggattc ggcgctgcgc gccgcatcgc
     4501 agcaggcgtc ccatcgcgca ttggcgttga ttgccctggg catggtcagc caccagagcc
     4561 agatgcatga acgcgcggca tcgtgtttac acgagggcat cgcgctggcc gcggacaggc
     4621 atgacccttg gcttgccgcg gcggggcggg cgatcctggc tttcgaactc gccaactgcg
     4681 gtgacttcga gcgtgcggcc gaagcagtgg attcggcgct cgccgtcgcc tcctcgttgg
     4741 acgaccgttg gttgcggtcg caggcactgc tgagccgcgg catcgtctat ggactgcaag
     4801 ggcgccatcg agaaggcgag gcatggctcg gtgaagccgc cgaatgtgtc acatcgccgc
     4861 ccaacgatgc cttccagcat ttttatgccc tgatcaatcg tgccttgctg cgtcactatc
     4921 tcggcagcga gtcccaagct gcacgggatt ggctggacgt tctcgatggc tgcgtgatgt
     4981 tgcagaacag gcgtggggct tcgggctgcg tcgagggtgc ggcctacctc accattcaac
     5041 gcggcgaacc ggcaaaagcc gtgcgttttc tcgcctcggc ggcacgcgtg cgcgaattga
     5101 ccgatgcccc gctgatgccg ttgtggcgaa agggccagga ggcgaccgag cgcgaggcgc
     5161 gcgacatgct cggcggggcg ttcgaggtcg cgtggcggga aggcgcctcg gcgcgcttcg
     5221 aggacatcat cggcgaggcg cgtgccctgc ttgccgaaat aaccggtcag ccggggcgca
     5281 gcggagccag caacccttcc ggatcgtagt gggcgcgcaa cccctgcaac cttgattgcg
     5341 tcgccgcgtc gtgtgcttgc gctttcaaat cggccggctc gcccgcaaaa ttcggctgca
     5401 tccccgtgcg tgagtacgct gcgaacgccg cgcgtagatc gcatacccag tcgcggatgg
     5461 ccgtgaaacg ttccgcggcg tcggtgtggc cgatgacatt gatgaagaat ggcgcggcgc
     5521 gatgagaaaa cgcggtggcg tcgcgggcga cgcgcgcggc cgcgccgccg agatggtgta
     5581 cgtgcacttc gcacagtgca tcggccggtt gcacggcgcg cggaaccagc gcttcgatca
     5641 gcccatcatc gaaggcgtcg aacagggaag tggtccagta atagtggtga cccgccggcg
     5701 cggcaggatc gaacatctgt tgccacgcca cgtaaggcat catgccttcg tggcggccca
     5761 acggttcggc ggcccgcgcg atcgatgccg cagcttgcgc gccgtgcccg ggatcaccat
     5821 tccagcaata cgccagcgcc actgcgcgcc gcccgtgtgc ttcgggcggc aggaacggca
     5881 atggcggcgc ggtggtgaac accagcatca cggtcaatgc atccgctgca tccggcgtga
     5941 acacgcgaaa cgcgcgcaac agcgcgggtc cggcctcgac cggatagaac acgatgccgg
     6001 catgcacctc gctgaccgga tgcagccgaa aggcgaagcg agtgaccacg cccaagcctg
     6061 ccccgccgcc acgcaaaccc caaaacaggt cggcgtgctg gtcttcgctg gccgtaacga
     6121 tgcttccgtc ggccagcacg acttcggctt tgagcaagtt gtcgatcgcc aggccgcagc
     6181 ggcgcatcag ccagccgacg ccgccgccca gcgtgtagcc gccgacgccg gtggtcgaaa
     6241 cgaagccacc ggtggtggca aggccgtgcg gctggctggc cgcatcgacg tcgcgccaca
     6301 acgcgccgcc ctcgacgcgc gcggtccgcg accgtggatc cacctccacg cggttcagcg
     6361 agcgcaggtc gagcatcaag gcgtcatcac gcacggccag tccggccacg ttatggccac
     6421 cgccacgcac cgtcatcggc acacgctcgc gcagggcgaa cttgacggcc gcgccgacgt
     6481 cctcggcatc ccggcagcaa gcgatggcca ggggacggcg atcgatcgca ccgttccaga
     6541 cgcgacgggc ttcgtcatag ccggcatcga cgggaagcag cagcgtgccg cgcagtggcc
     6601 cctggaggtc tcgccacggg aaattttgag tgcggctgga atgggaaacc atgcgcgccc
     6661 tccttcgccg ggacgatggg cggctgggga cgcactatag ccccggcccc ggaaaacagt
     6721 catgacgggt ggatagggat ttcgtaaggc ctgcaaggcg ggcggaacgt ggctaccgcc
     6781 ccggcgcgcg gctaaggtac gcgcatgttc atcgacgtgc ccagccggcg ccgagcgcac
     6841 ccccgttggg tcacgccctt cctggtggtg acctgcgtcg cctgctacct gtggctgatg
     6901 ctgtcctcgc cggcgacgcg cgaggtggtg ctgggcgact ggggcaccgt acccgcgcac
     6961 ctgttccggc gaggcgtgtt cctcgagggc tggctgtggt tgcgtccgct cacctcgctg
     7021 ttcatgcacg cggactggct gcacctggtc ggcaacatgc tgttcctggt gatcttcggc
     7081 ttgccggccg agcgtgcgct cggctcgaga cgcttcctgg cgttgttcct ggttggcggc
     7141 gccttcgcga acctggtcgg tgcctggacg ttgtctgcgc aattgcggcc gatcgtcggc
     7201 tgcagcggcg ctgtgtcggc gatcctgggc gcctacctgg cattgtttcc acgcgcgcat
     7261 ctgggactgg tgttgccgct ggggctgtac ctccagttca tccgggtgcc ggcagcactg
     7321 ctgatcggga tgtgggtgct gctgcagctt ttgttcacct gggccggacc gggcttcggc
     7381 acggtggtgt ggtgggcgca catcgccggc ttcggcttcg gcatcctgtt cgcagtggtt
     7441 tcgcgcgggg cggtggaacg gcgcctccgc gaaagaatgt actagaggag tgcctgctct
     7501 tcgtcacgcc gccgcgacag cgccgcctgc gcgaaccgtt ccagcaaggc gcgtgccgcg
     7561 gtggtttcac ggacgtcgcc gcgcagcgcg tccacatcga cgccttcgtc gcgcagcaca
     7621 gcggcgcgcg catcgatata ggcgcgcatc gccgcggcgc tgaattcggg gtggaactgg
     7681 gtcgataccg catgcggcgc atagcgcagc aactggtgcg gatcgaggtt cgaggacacg
     7741 agcactgcgg cgccacgcgg cggctccagc accgcctgcc ggtgggtcgc atgcgcggga
     7801 aacgtctcgg gcaaatcatc gagccagtcg ccgccgtcac ggaaatgccg tgtcatgatt
     7861 cgagttccca tctcgcggcc cgccggcaac cagccaaccg tgccgcccag cgcgtgcgcc
     7921 atcaactggt ggccgtagca cacgccgaac aagggcgtgt cggcatccat ggcatcccgg
     7981 atccaggcgg ccgtgcgctc gctccagtcc gcgcgctcgg tgaccatcgc tgccgagccg
     8041 gtgatgaccg cgccggcaac cgaatccggt gcgggcagcg cggcgccttc gtcgacccgc
     8101 accacccgaa gctgggcggg cgggacgcgc atcgcgtccc ggaaccagtc ggcgaatccg
     8161 ccgaagcggg cgtggatgga ttccggcgct tcgccggtct ggaccaggag caggtggggc
     8221 atgttcactg ccgaacaggg agtacgcgca gcatagcgcg tgggcgctgc atcagcccgc
     8281 ggcgggcagt accgacagca cttcctcgac ggtggtcaag ccgcggcgaa cctgggtcgc
     8341 cgcggatatc cgcagcggct gcaggccatc ctccagcgcg aagcgggtga agccatcgag
     8401 atcgagttgt gcggtgatca tgccgcgcag gcgtggcgtg accggcatca tctcgtaaac
     8461 gccggtgcgt ccgaggaagc cggtcttgcg gcattcgagg cagccgaccg gttcgtacac
     8521 ttcggcgggc gcggggatca cccagccgcg ggtcagcacc gaccacgtct gcggatcctg
     8581 cttcgccttc cgcttgcagt gcgggcagag ggtgcgcacg agccgctgcg cgaccacgcc
     8641 cgaaagcgtg gactggatca ggtagtgcgg cacgcccaag tcgagcaggc gggtgatcgc
     8701 cgacggtgcg tcgttggtgt gcagcgtcga gagcaccaga tgcccggtga gcgatgcctg
     8761 caccgccatt tgcgcggtct cgaggtcgcg cacctcgccg accatgatga tgtcgggatc
     8821 ctgcctgagc agggtgcgta cgcccgccgc gaagtcgaag tcgatcgccg gttgcacctg
     8881 cgactggttg aactccggcg ccaccatttc gatcgggtcc tcgatggtgc acacgttgag
     8941 ttcggggcgc gcgaggtgct tcaacgtcga atagagcgtg gtggtcttgc ccgaaccggt
     9001 gggcccggtg accagcacga tgccgtgcgg gcgttccacc atctcccgcc aggttttttc
     9061 ttcctcgcca gagaacccca actgcgagaa ctgcttgacc acgatgtccg gatcgaagat
     9121 ccgcatcacg atcttttcgc cgaacgcggt cggcatcgag gagatgcgca actccacctc
     9181 gcgtccgccc gccgagcgtg tcttgatgcg gccatcctgg gggcggcgct tttcggcgac
     9241 gtccagccgc gccaggatct tgattcgggc ggtcaccgcg gtcatcaccg cgggcggcag
     9301 ctcgaacacc ttgtgcatca cgccgtcgat ccggaagcgc atcagcgcgg cttcgcggcg
     9361 cggctcaagg tggatgtcgg aagcgcgctg ctcgaacgcg tactgcagca gccagtcgac
     9421 gatatggacg acgtggcggt cgtcggcgcc gatttcgccg gccttgccga gctcgagcaa
     9481 ttgctcgaag ttcaggatcg ccgagccatc catcgcatcc tgcttggcct cgcgcgcctt
     9541 ggcgatcgag cgctggacgc cgtagaactc catcaggtag cggtggatgt ccagcgggtt
     9601 cgcgaccacg cgcttgacct cgcgcctcag catctgcgac aggtcggcgg cccaatggtc
     9661 gtcgaagggt tcggcgctgg cgaaggtgac cgtgccgtgg ttgaccgcga ccggcaggat
     9721 ccggtggcgc tgcgcgtagg cctggctcac ggcctgggtc accgccgccg cgtcgatctt
     9781 cataggatcg atcttgaggt acggcaggcc cgccttgtcg gccagccaca ccgtcagcga
     9841 ctcgaccgtc agcggcttgc cgcggtcggc gcggttctcg ggtttgccgc tcgagatcac
     9901 gaccagcgga tgcagttcga cggcaccgcg ccccgcacgg ctgctgcccc ggatgcgctt
     9961 ggcgtcttct gccgccaaca gcccgtcggc gaccagcgcg gccagcacct cgcccagttc
    10021 caggcgcccg ttccggccga gggtcgctgt cgaattggga attgccgcca tgccgtgccc
    10081 cgcctgcgga tggacggccc gaaaccgcgg tggatcgggc cccgcggcta tactaacgcg
    10141 ctttgtgcgg cgcaccgggg acgcagttgg caggtccgac tttccagcag gtcatccaga
    10201 ccctgaaccg ctattgggcg gaacaggggt gcgttcttct gcagccgctg gacctcgagg
    10261 tcggggcggg caccttccac cccgccacct tcctgcgcgc gctgggcccg gagccctggg
    10321 ccgcgaccta cgtgcagccg tgccgccggc ccaccgacgg ccgctatggc gagaatccca
    10381 accgcctcca gcagtactac cagtaccagg tcgtcctgaa gccgaatccc gatgacgtgc
    10441 tggaacgcta cttcggcagc ctgcgcgagc tcgggatcga tccgctggtg cacgacctgc
    10501 ggctggtgga ggacaactgg gagtcgccca cgctgggtgc ctggggcctc ggctgggaag
    10561 tgtggttgaa cgggatggaa gtcacccagt tcacctattt ccagcaggcc ggcggcctcg
    10621 agtgcaagcc ggtgacggcc gagatcacct acggcctcga acgactcgcg atgtacctgc
    10681 aaaacgtcga cagcgtgttc gacctggtat ggaccgaagc gccgcacggc atcgtcacct
    10741 acggcgacgt gttccgccgc aacgaggtgg agcagagcgc ctacaacttc gagcacgcgg
    10801 atacgaccga gctgttccac cgcttcgatg cctgcgaggc cgaagccaac aagctggtgg
    10861 cgctgcacct gccgctgccg gcctacgagc aggtgatgaa ggccagccat gccttcaacc
    10921 tcctcgatgc ccgccgcgcg atttcggtca ccgagcgcca gcgctacatc ctgcgggtgc
    10981 ggaccttggc gcaagcggtc gcgaaggaat gggtcgcgcc gcgcgaggca acgaacgcgg
    11041 aggccgcaca tggctgagcg ttcgctgctg gtcgagatcg gcgtcgagga actgcccatc
    11101 catgcggtgg acgatcttgc gcaggcgttc gcgcgatcaa tgtgcgaggg cctcgtcaag
    11161 cgcggcatcg cggccgacgt ggaacacgcg cgcgccttcg cgacgccccg tcgcctggcg
    11221 gtttgggtgc cggacgtggc cttcacccag cccgagcagc gcagcgaaag cttcggcccc
    11281 tacgtcaaca ttgcgctgga cgccgaaggc cagcccacca gggcgttgct cggattcgcg
    11341 aacaaggccg gtgtggaatg gacgcaactg gaacgtggga cggacgccaa gggggaacgt
    11401 ttcgtgcatc gccgggtgtc gccggggcgg accaccgccg agctggtgcc ggagatcgcc
    11461 ggtgaagccg ctgccgcctt gccggtcgcc aagcccatgc gctggggcga ccacgagtcg
    11521 tccttcgtgc ggcccgtgca ctggctggtg atgttgcacg gcgaccaggt catcccgggc
    11581 gagttgctgg gcctgaaagc cggtcgccag tcgcgcggac atcgattcat gcacccgcat
    11641 ccggttcacc tcgccgacgc cgacagctac gccgattccc tgcgcgcggc taacgtgctg
    11701 gccgatccag ccgagcgccg ccagcggatc cgcgacgaag tcgcgcgcgt cgcgcctgcc
    11761 ggttgcacgc cgcgcctcga cgagccactg ctcgacgaac tggccaacct cagcgaatgg
    11821 ccggccgcga tagcctgcac gttcgaccgg gagtacctcg acgtgccgca cgaggcgctg
    11881 gtcaccacca tgcgcaccaa ccagaagttc gtgccggtgt tcgatgcgga aggaaggctg
    11941 accgagcatt tcatcggcat cgccaacctc gaatcgaaag atcccgcgca ggtccgccac
    12001 ggctacgagc gggtgatccg tccgcgcttc gccgatgcaa agtacttctt cgacgaggac
    12061 ctgaagacgc cgctggggtc gtatcggaag cttctcgaag acgtggtgtg gcagcgtgcg
    12121 ttgggcagcc tgtgggacaa gaccgtacgc gtggcggaac tcgccagcgc gatcgcggcg
    12181 cgcctgttcg cacagggcat cgatgtggat gcggcacagg cggcccacgc ggccggcctc
    12241 tcgcgctgcg acctgttgac gcggatggtg ggtgagttcc cggagttgca gggcgtgatg
    12301 gggcgccact atgcggcggc gcagggcgaa cgggccgacg tcgccgatgc actggacgag
    12361 ttctaccgcc cgcgcaatgc cggcgacggc atcgcgcagg gcacgctcgc gcagtgcctg
    12421 tcgcttgcgg acaaactgga cacgctcgcc ggcatcttcg cggtcggcat gaagccgacc
    12481 ggcaacaagg acccgttcgc gttgcgccgt gccgccctgg ggctggcacg gacgctgatc
    12541 gagggcgggc tcgatgtcga tgtggaagcg cttttgccgg aacacgcagc gctgatccgc
    12601 gccgacatgc ggaaagcagc aattgccctg aagcgagatc aatctggcgg acgagttgct
    12661 cacctcgagg ctggggatgt cgtcactctg cgtattgcgg aagtgtatcc cttcatcctc
    12721 gatcgcctgc gcggctatta caccgagcag ggtttcacgc cccagcagtt cgaagcggtg
    12781 gccgcggtgc ggcccgcgag cctgcttgat ttcgaccgtc gcctgcgcgc ggttgcgcag
    12841 tttgcccggc aacccgaagc cgaacagctc gccgccgcca acaagcgcgt cgccaacatc
    12901 ctgcgcaagg aaggcatcga cgcggaagcc gcggcacggc atgaactgga cgcggagctg
    12961 ttgtgcgaac cggccgggca ggccttggcg caggcgctgg atgaa
//
LOCUS       DASSNH010000114        23260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_141934, whole genome shotgun sequence.
ACCESSION   DASSNH010000114 DASSNH010000000
VERSION     DASSNH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 23260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23260
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141934"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /gene="leuS"
                     /locus_tag="VFK00_04025"
     CDS             complement(<1..1821)
                     /gene="leuS"
                     /locus_tag="VFK00_04025"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7592156.1"
                     /translation="MQEPRDRSAYDPGTVEAAAQKFWADTRAFEVEEDASREKYYCLS
                     MLPYPSGALHMGHVRNYTIGDVISRFQRMQGNNVLQPMGWDAFGLPAENAAIKRGIPP
                     AKWTRENIAHMRGQLKAMGFAIDWSREFATCDPDYYVHEQRMFVRLFKQGMVYRKNSV
                     VNWDPVDQTVLANEQVVDGRGWRSGAVVEKREIPQWFLKITAYADELLDGLDTLPGWP
                     DAVKTMQRNWLGRSEGLEIRFDVEGGGSLEVFTTRPDTLMGVTYLAVAAEHPLAQEAA
                     RNNPALQPFIDECRHGGVSEAELETREKTGVATGLFALHPVTGERVPVWIANFVLMGY
                     GTGALMAVPGHDQRDWEFATKYDLPIRMVVVSDAVRDAIRELGRDAAENIDAMSAALG
                     QSKPIDVAEPRPVEDVLEEFEDRIITEGPFLEHGTLVNSVDYDGLDYRQAFDALAERF
                     EREGRGRRQVNWRLRDWGVSRQRYWGCPIPIIHCPKCGAVPVPEDQLPVKLPEDVAFS
                     GVQSPLKSDPEWRKTTCPECGGAAERETDTFDTFMESSWYYARYCSPGAATQVDARAN
                     YWLPVDQYIGGIEHAILHLLYFRFYHKLMRDAGLVHSDEPA"
     gene            2049..3425
                     /locus_tag="VFK00_04030"
     CDS             2049..3425
                     /locus_tag="VFK00_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7592157.1"
                     /translation="MRIEGIRRLPLTLAIAALTITTAQAAEAPVANAAPAPELTVIHC
                     AHLIDTVAGKMLGATSIVIDGQRIKEVVPGSASRDGAKVIDMPANDTCLPGLVDAHTH
                     LTSQFSKSTYSDQFRLNPADYAIQSTVFARRTLLAGFTTVRNLGESDNSSIALRNAIN
                     KGEVPGPRIFTAGKFIGTTGGHADPTDGYRWDLQGDPTPKDGIIDSPADAWKAVRQHY
                     KDGADLIKIMPSGGVLDESSSSENPQMTLAEIKAVVAAAHDYGFTVAAHAHGAEAIRR
                     AVIGGVGSIEHGTFMDDEDMKLMKQHGTWYVPTIIAGEFVMGKAKEGWYPPQVARKAL
                     EVGPKIMATAGKAYKAGVRIAFGTDAGVYPHGGNAQEFVYMVQAGMPPMYVIQAATTH
                     AATLLKHQDDFGSITTGKYADVVAVPGNPLDDISLMKKVDFVMKAGTVYKQQGKPTDA
                     DLESSLGM"
     gene            3451..4356
                     /gene="trxA"
                     /locus_tag="VFK00_04035"
     CDS             3451..4356
                     /gene="trxA"
                     /locus_tag="VFK00_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7592158.1"
                     /translation="MNFNPKPDDAAPADSFVFEAGESNFEADVLQASLQTPVLVDFWA
                     TWCGPCKTLGPILEKLADEYAGGFRLAKIDCDKEQQLAGMFGVRSIPTVVLIQGGQIV
                     DAFSGALPESQVREFLKRHGVEPASRIEAPAQAVEDGAAMPAETPQAAIARIEQALAA
                     DPDNAGLKLDLALARARGGDTANAQSTLDTLPVDLAEDDRAKALAALLAMHQSLAAIP
                     PAGELLARVERDPRDFAALDGLGVRQLLGGDAADAMQRWLAILAADRSWNDGLARKRL
                     VDAFRIVHDEALVSTTRRKMSSLLF"
     gene            4365..4832
                     /locus_tag="VFK00_04040"
     CDS             4365..4832
                     /locus_tag="VFK00_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HET7592159.1"
                     /translation="MRARTLRRLMNLWPPFLANGIRVLEISDDWRRARVALKLRPWNR
                     NHVGSQFGGNLFAMTDPFWMVLALHRLGPDYYVWDKAASIEFVAPAREDVFAGFHLDD
                     DAVALLRHEAASGDKVLHWFDTEVATGSGETIARVRKQLYVRLKPRARSARPS"
     gene            4997..7192
                     /locus_tag="VFK00_04045"
     CDS             4997..7192
                     /locus_tag="VFK00_04045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HET7592160.1"
                     /translation="MNDATPHQLEVPGYRLVRPIGTSGVASVYEATQRSLERQVAIKL
                     WRPLDDNALGRFEQLLRTNARLSHPNIVGIHQIGRTEDGRLFHSMPFLLSIGLSAHNL
                     RFKPLKVAGLLRALLDALGHAHRRGIVHGGIKASNVLMDQRGHVRLADFGIARCAAEL
                     GLRHASAAAYLSPEQARGEPPGQRSDLYSIGILAFELLTGAPPFQGGDAVSTAIAHVE
                     QPIPRLPPLVGGWQAWIDKALAKSPEQRFQGAQEMADALSAIDGRRRIRPVAVPVGSR
                     LRAPTWAIAGAAVGAALVLAVGAWATWRSFPRNDAATLPGPVVIAAKAATASLPASAP
                     PAASTAPSTQDRAYALVKEADALRTRGRLFAPAGNNAADKYLAALALDPGNPAATAGI
                     DAMLATLRQRLAAAWSDGNLDRTAAMLKRCDLLASHAGPVARRDWRTDREDFAEHVGD
                     AVVAAADKRDIVRLAALKPLAQALPATYPAGFDLAVAERRAATPVAGDRMHDERGPTL
                     VYVPASGGAPAFAIERVEVTRGDYAAFVRATHRPDSRCLEAYNPFSRLRHLSWQDPGF
                     KQGDDHPVVCVSWNDAVAYAAWLSKETGQAYMLPSSGEWLRAAQGLPGATPCQLGNVD
                     DTSRQSAMDNDRLSCNDGAARTAPVGHYLPSGAGVYDLYGNVSEWVADGSSGERVFRG
                     LSWRDGGQGKPLARRGTADSDVGYTNVGFRVVRVIDDAHPAPPVARASR"
     gene            7725..9368
                     /locus_tag="VFK00_04050"
     CDS             7725..9368
                     /locus_tag="VFK00_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HET7592161.1"
                     /translation="MKANRLAVALASLLLCSAPAFAAPPASASSSAQSDSSGGQHESL
                     IKRLSQPMSSTTHGTVTVEGKTISYEAVAGTLVLDGTGAKESTPEVAMSYFAYFKQGV
                     DPSNRPITFIYNGGPGSSTMWLHMGAWGPKRVVTNDHTHTPAAPYQLVNNDYSLLDAS
                     DLVFIDMPGTGFGRLLPQGKDDDARAKDRKDLAKQIWGIDGDADSFAHFITQFLTKYD
                     RWNSPKYLFGESYGTTRSAVLSNILQTEYNVDLNGVMLLSQILNFGLSIDGADMNPGI
                     DMSYVLGLPTFAATAWYHHKLPKFNNGSLAPLLKEVTQFATTDYEQALSAGSTLSAAK
                     KQQIAGKLHDYTGLPVDYLLKADLRVTGGMFEHELLDDSDTTTGRLDTRFSGATMDPL
                     GKESDYDPQSAAISAAYVAAFNSYVRNDLKFGEGMKYRPSVYGADDFRWDWKHRAPGQ
                     RYSRGGTPNVMLDLAVAMKYNPDMKVRLFGGFYDLATPYYTAIYEMQHLPIPEKLQKN
                     ISYGFFPSGHMVYAHTPSLKKLHDDAVQFIDASDNLGNK"
     gene            complement(9458..10102)
                     /locus_tag="VFK00_04055"
     CDS             complement(9458..10102)
                     /locus_tag="VFK00_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592162.1"
                     /translation="MELDEMRLAWQELGAKLEQQRTLNLRLIRNQGLDKLRRGLRPLV
                     WGQSLQLLIGVALATWAVTFWVSHRQVLHLLVCGLLMQGFGLLMILSAARVLELVRRV
                     DHGAPVTVIQHQLAQLRKWRVKVEAPIDAIVGSFIWIPAFVMAFASSGFDPWAPGLGR
                     WAVLSGAASLGLVAMGIWLALRLGYGRRLENHAAGSSVLAAEDALDEIARFEQE"
     gene            complement(10089..10643)
                     /locus_tag="VFK00_04060"
     CDS             complement(10089..10643)
                     /locus_tag="VFK00_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7592163.1"
                     /translation="MADQQRQRRFEVLLREHQRIVFKIAAAYATGAPDRDDLAQEIAA
                     QLWRSFGRFDERRAKFSTWMYRVALNVAISHARHAHRAGAGHAEPFEEPHVASVASTD
                     EAPADGLERAERIAALYAFIDGLDPLNRALVLLYLEDRSYAEIAEVLGISVTNVATKL
                     NRIRQKLRPQATAAATEEVDYGTR"
     gene            10769..11452
                     /locus_tag="VFK00_04065"
     CDS             10769..11452
                     /locus_tag="VFK00_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET7592164.1"
                     /translation="MPALHVKRYLITGLLAFIPVWITWVVFEFVFTLLAGVGAPLVNG
                     IIGLIGLGAPVAAQVLHRDWVVSILAFVLTLLALYVLGWLSTKVLGRRVLGAVDALLE
                     RIPLVHTIYGGVKKLMTLMRKKPGGTQRVVLIDFPSPELKSIGFVTRTFVDAAGCEVA
                     AVYVPTTPNPTGGYLELVPTERLVATDWTIDQAMAFLVSGGAVGPEQLPAVSPAPAPL
                     RAPVETTGA"
     gene            11449..12039
                     /gene="thpR"
                     /locus_tag="VFK00_04070"
     CDS             11449..12039
                     /gene="thpR"
                     /locus_tag="VFK00_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7592165.1"
                     /translation="MSGPAAPAATGETARCFLALLPDSASRDALLRFRQALEASRSGA
                     ARGVRWLDAASLHVTLRFLGDSTRAQVEFLKHMLAALARQLPAFDARRCAIWPNRARP
                     RLLVLELDAPGELRALAEESEGLARKAGFEAEPRAFRAHLTLARLRPGCTLEVPSVPL
                     PRLAFESLSLMQSELQATGARYQPLATVPLSGSRKC"
     gene            12264..13646
                     /locus_tag="VFK00_04075"
     CDS             12264..13646
                     /locus_tag="VFK00_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7592166.1"
                     /translation="MTFESLGLAPALLRALAEQGYANPTPIQVAAIPLVLNGHDLMAG
                     AQTGTGKTAAFALPLLQKLCVDQANRDSKQPRKPRALILTPTRELAAQVHDSVRSYGK
                     HLRVYSAAIFGGVGMGGQVMSLRRSLDIIVATPGRLIDHMDRRNVDLSCIDTLVLDEA
                     DRMLDMGFAPALKKILGVLPKQQRQTLLFSATFVPEIKALAAQFMHDPREVQMAQPNA
                     VAVGVHHRVHPVDAEKKRDLLLHLLAADSRRQTLVFARTKHGSDKLCKYLDQHGFRVA
                     AIHGNKSQGQRTRALADFKNGKTTVLVATDIAARGLDINQLPAVVNYDLPMVAQDYVH
                     RIGRTGRAGSEGLALSLVSREEEGLLRDIRRLLKQELAIEPVEGFEPTWQPRGERDDV
                     RSAKAGPRPGKPRHARQVRRDGIAKPGSRWKQASGRTRGKPSNTAAHAEGNVAKRGRG
                     KRPGWQSGSR"
     gene            13849..16002
                     /locus_tag="VFK00_04080"
     CDS             13849..16002
                     /locus_tag="VFK00_04080"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HET7592167.1"
                     /translation="MHKHLLTPIALAIGAALAVAGCSNNAQNQNGVAPNGAASAPVPA
                     QTAAAPAAGSTVAIMPAQPIAFDLDDQYNPCNDFAEYVNAKWNKANPIPADETRWGAF
                     GILHERSLQQQKQILETAAQDAKDGKGNELQQKLGHLYAAGMDTATINKLGYDPIKPQ
                     LAAIDALKTPADIADFITTSYDNGDSYVFNFGSSADFKDATKQIGYVFQDGLGLPTRD
                     YYLSPKYKDIRAAYLKYISKSLQLVGTPAADAKKQADEVLKFETELAKASLAPVELRD
                     PKNQYHFVTVAEADKVSPHFSWDKFFKGQGVDIGKGFSLSQPKFIAEFDKLLAHAPAS
                     QWQAYLKFHLISSASPALSQAFQDNQFDFYGKTLQGQLEQKDRWKRVIGAVNGAMGMG
                     LGELYVAKYFPPESKQRAEELVTNVRAALKEHIENLDWMSEATKKKALDKWSLFLPKI
                     GYPDKGEWRDWSGLTIQPDKWYADLEAASKYNYHYDIGKIGKATDRKEWHMTPQTINA
                     YYSPSTNTINFPAAILQPPFFYAKGDDALNYGGIGFVIGHESSHGFDDEGSQFDGHGN
                     LVNWWTPQDKKQFDERTAALVKQYDSYTPIEGKPDLHVNGKLTLGEDIGDLGGLNIAY
                     TALQTALKQNPQEADQKIDGMTQNQRFFLSSARVWEGTSRDKTSELLLNVDPHAPAKI
                     RAFASAANMPQFAQAFQCKPGDKMMHTGDKLVKIW"
     gene            16306..18624
                     /locus_tag="VFK00_04085"
     CDS             16306..18624
                     /locus_tag="VFK00_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HET7592168.1"
                     /translation="MPGIKHRKHAAARCKPPLALSTLMTGIAFALPSLAASGPAAPAG
                     LAGAAQLPTIHVRGTRNPYKIDVVDSPKFTQSLLDTPQTINVIGKEIIEQQGATTLTE
                     ALRNSPGVGTFYVGENGSTSTGDAIYMRGFDTSGSIFVDGVRDLGSISRDVFDVEQVE
                     VTKGPDGTEYGRTAPTGAINMVSKQPRSKDQVEASVGYGSANRRRATADWNQVIDRHT
                     AFRLNVMGQKSGVAGRDEVREDRWGIAPSLAFGLGTPTTVFVDFLHVTQDNVPDGGVP
                     TIGLPGYATPDPSRPFLADAPKVDPSNFYGTDADHDDVEADMFTVIASHDFDNGVALH
                     DTLRWGRTHEDYLLTSFMGRADRLLTPDPADLATWTIARSNPTFKDQTNRILTNQTNL
                     TAHLGSGAVEHDLSAGIELTREEIRTGGTGALDDSAWTPANLYRPDPHAATGLVIGDT
                     GAWSRGRTDTAAAYFFDTMHFGERWLVTAGARVDHYTTGFHSVEPCESSRRAPSCNGS
                     PAGTPLPSVDADLGGNLFGYKLGIVYKPVADGSIYANFAAAAEPPGGNNLELSSSASS
                     ADNPAFDPQHARTVEVGTKWDVFDGQLRLTAAAYRTVVTNLVEQDPVDLQYYQIGRQR
                     VQGIELGAVGRISQNWAVSAGYTLMDAAVTRGAAVAEDGSDALPYTPRSAFTAWSSWR
                     LPHHVTLAAGARYSGEMRRGTDGAVGTPAFTEAYWVLDAMASYAINRRMRLQLNVYNL
                     FDRHYVAAINKSGYRYTPGMPRSALLTLNIHY"
     gene            18630..19307
                     /locus_tag="VFK00_04090"
     CDS             18630..19307
                     /locus_tag="VFK00_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HET7592169.1"
                     /translation="MLLHIEHVLDADTVTRMRAHLDAAEWTDGRETVGPQGALVKRNL
                     QLPDASPLRAQLGREVLAACARHADFHAATLPVKILPPRFNRYADGGSYGAHVDGSVM
                     ALADGSQLRSDIACTVFLAEPDDYDGGELVVEDTYGEHEVKLAAGDAIVYPASSLHRV
                     EPVTRGARLAAFFWVQSLVRDEAQRRTLLQLDSATRRLAAAAADADAVKQLAGVYHNL
                     LRQWAET"
     gene            19459..22290
                     /gene="rapA"
                     /locus_tag="VFK00_04095"
     CDS             19459..22290
                     /gene="rapA"
                     /locus_tag="VFK00_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HET7592170.1"
                     /translation="MDYTPGQRWLSEAEPELGLGTVLRTDERAVQVLFAKSGVVRNYS
                     AHGAPLTRATFRVGQRVAGRGVSFVVERIDLREGLLVYSGEKRELGEGQLDDEQSLSH
                     ADARLISARIDRPHHYELRKQALLARAAARRSEAWGILSARVDLLPHQLGVVATCMER
                     EHPRALLADEAGLGKTIEAGMLMARMLATGRAARVLILLPDALVVQWFVELRRRFQLE
                     FAILDEERCNAVATADPAGNPFEAEQLVIVSLAWLAADPQRTAQAVEAGWDLLVVDEA
                     HHLEWTPEAASPEYLAVEALAAISPSVVLLTATPEQLGRRAHFARLRLLDPARFDDLD
                     RYLGEADRYAALSPVAERIAAGDPLDEAARTDLCNRFPHDPALHALVAGYPESAEELL
                     SALIDRHGTGRVMYRSRREGVGGFPARQLEIGDAAELAPGARADLLAEFEDDMHATPV
                     AAQRALDYSNDPRIAWLLALLEGHPEDKFLLLASTRAKVEALDAALRTRTGTGVALFH
                     EGLTLLQRDRAAAWFAQPDGARLLLCSEIGSEGRNFQFTHRLVLWDLPLDPDLVEQRI
                     GRLDRIGQRHAVVLHVPMLPGTAQEALERWHADGTRVLRESPADGRELLRRFCCDVEA
                     VARAHADGGESEDPELDSLVATTAAVHAELSQSIRDGRDRLLELATRHSGGAALLDAM
                     RKADRDEQTHALIQQCFEPFGVHVEDLGGDAFLLDPEFLSTDALPGFVDGPRRATFDR
                     VLALAREDLDLPRMDHPLVLGAFELLLTGEFGNAAFLVDPALPSRSVLLEALYVVECV
                     ADVRLDAPRFLPPTPISVCVDSRLASRDYTPSPPSLARARERPLDISRYRRYLSQLVP
                     PMLDKATALARERAAEVTGEAAARAGATLDARVERLRALAAINATVRPEEIAGAEATR
                     EALLAALGRARVRLDAVRMAVSPDFLTLR"
     gene            complement(22515..>23260)
                     /gene="secF"
                     /locus_tag="VFK00_04100"
     CDS             complement(22515..>23260)
                     /gene="secF"
                     /locus_tag="VFK00_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7592171.1"
                     /translation="AVVQSMGGTREISIRLQPPKQADKAHKQSPDEVAAEVSKRVLAA
                     LAAAHPGVKLAHPPDFVGPEVGQELKTSGIYAAIVVIIGIMIYLWARFEKRFAFAAVV
                     SEIHDTVITVGIIALVQREFDMTELASILAVIGYSINDKVVVFDRVRELFRLTRKADT
                     PQILNRAINNTLSRTIITSLFTAITMATLWLFGGSAVHGFAITMLIGILVGTLSSIFF
                     ASPMLLWFGVTKKDLMPVTRENPELARRP"
BASE COUNT         4045 a         8138 c         7460 g         3617 t
ORIGIN      
        1 cgcgggttcg tccgaatgca ccagccccgc gtcgcgcatc agcttgtgat agaagcggaa
       61 atacaggagg tgcaggatcg cgtgctcgat gccgccgatg tactggtcga ccggcagcca
      121 gtaattcgcg cgtgcatcga cctgggtggc ggcacccggc gagcagtagc gggcgtagta
      181 ccaactcgat tccatgaagg tgtcgaaggt gtccgtttca cgctcggccg cgccaccgca
      241 ctccggacac gtcgtcttcc gccattcggg gtcggatttc agcggcgact gcactccgga
      301 aaacgcgacg tcctcgggaa gcttcaccgg caactggtct tccggcaccg gcaccgcgcc
      361 gcacttcggg cagtggatga tcgggatcgg gcaaccccag tagcgttgcc gcgaaacgcc
      421 ccagtcgcgc aatcgccagt tcacctggcg ccggccacga ccttcgcgct cgaagcgctc
      481 cgccagcgcg tcgaatgcct gccgataatc caggccgtcg tagtcgactg aattgaccag
      541 cgtgccgtgc tcgaggaaag gaccctcggt gatgatgcgg tcctcgaact cttccagcac
      601 gtcttcgacc ggccgcggct cggccacgtc gatcggcttg gactggccga gcgccgcgct
      661 catcgcatcg atgttctcgg ccgcgtcgcg cccgagctcg cggatcgcat cccgcaccgc
      721 gtcgctgacg accaccatcc ggatcggcag gtcgtacttg gtggcgaact cccagtcgcg
      781 ctggtcgtgc ccaggcaccg ccatcaaggc gccagtgccg tatcccatca ggacgaaatt
      841 ggcgatccac accgggaccc gctcgccggt caccggatga agcgcgaaca gcccggtcgc
      901 gacgcccgtc ttctccctgg tctcgagctc ggcttccgaa acgccgccgt gacggcattc
      961 gtcgatgaac ggctgcagcg cgggattgtt gcgcgccgct tcctgcgcca gggggtgctc
     1021 ggccgcgacc gccaggtagg tcacccccat caaggtgtcc ggccgcgtgg tgaagacctc
     1081 gagagaaccg ccgccctcca cgtcgaagcg gatttccagt ccttccgagc gcccgagcca
     1141 gttccgctgc atggtcttga cggcatccgg ccagccgggg agcgtgtcaa gcccatcaag
     1201 caattcatcg gcgtacgccg tgatcttcag gaaccactgc ggaatctcgc gcttctcgac
     1261 caccgcaccg gaacgccagc cgcggccatc taccacctgt tcgttggcca gcaccgtctg
     1321 gtcgaccgga tcccagttca ccacgctgtt cttgcggtac accatgccct gcttgaacag
     1381 ccgcacgaac atccgctgct cgtgcacgta gtagtcggga tcgcaggtgg cgaattcgcg
     1441 cgaccagtcg atcgcgaagc ccatcgcctt caactgcccg cgcatgtgcg cgatgttctc
     1501 gcgcgtccat ttcgcgggcg ggatgccgcg cttgatcgcc gcgttctcgg ccggcagccc
     1561 gaatgcatcc cagcccatcg gctgcagcac gttgttgccc tgcatccgct ggaagcggct
     1621 gatcacgtcg ccgatcgtgt agttgcgcac gtggcccatg tggagcgcgc ccgacgggta
     1681 cggcagcatg gaaaggcaat agtatttctc gcgcgaggcg tcttcctcca cctcgaaggc
     1741 gcgggtgtcc gcccagaact tctgcgcggc cgcctcgacg gtgccggggt cataggcgct
     1801 gcggtctcgg ggttcctgca tggctgggcg gcgaagcggt ctgatcgggg atggatcagc
     1861 ctaccgcagt gcagcaaaag cgccaagcgg cactcgagcg gcccttcatt cgtttaaggc
     1921 gtgacgccga acgcgcgcag gcgaagtacg ggcgtttcac cgcgcgctgg atgctgcaac
     1981 cgagcgcgtg cgacagccgc tcgcggcgtg gctatgatgc cggcttccat ccgacccgag
     2041 gggacaccat gcgcatcgaa ggcatccgca ggctgccgct gacgctcgcg attgctgcgt
     2101 tgacgatcac caccgcacag gcggccgagg ctcccgtcgc gaatgcggcg cccgcgcccg
     2161 agctgacggt gatccactgc gcgcacctga tcgacacggt ggcaggcaag atgctcggcg
     2221 ccaccagcat cgtcatcgac ggccagcgca tcaaggaagt agtgccgggt tcggcaagcc
     2281 gcgacggcgc gaaggtgatc gacatgccgg cgaacgacac ctgcctgccc gggctggtcg
     2341 acgcgcacac gcatttgacc agccagttca gcaagagtac ctacagcgac cagttccgcc
     2401 tgaacccggc cgactacgcg atccagagca cggtgttcgc gcggcgcacg ctgctggcgg
     2461 gtttcaccac cgtgcgcaac ctcggcgaaa gcgacaattc ctcgatcgcg ctgcgcaatg
     2521 caatcaacaa gggcgaagtg ccggggccgc gcattttcac cgccggcaag ttcatcggca
     2581 cgaccggcgg ccacgctgat ccgaccgatg gttaccgctg ggacctccag ggggacccga
     2641 cacccaagga cggcatcatc gattcgccgg cggatgcgtg gaaggcggtg cgccagcatt
     2701 acaaggacgg cgcggacctc atcaagatca tgccttccgg cggcgtgctg gatgaaagct
     2761 ccagctcgga aaatccgcag atgacactgg ccgagatcaa ggcggtcgtc gccgccgccc
     2821 acgactacgg cttcaccgtg gccgcgcacg cacacggcgc ggaggcgatc cgccgcgcgg
     2881 tgatcggagg cgtgggttcg atcgagcacg ggaccttcat ggacgacgag gacatgaagc
     2941 tgatgaagca gcacggcacc tggtacgtgc ccacgatcat cgccggcgag ttcgtgatgg
     3001 ggaaagcgaa ggaaggctgg tatccgccgc aggtcgcgcg caaggcgctc gaagtcggcc
     3061 cgaagatcat ggccacggcg ggcaaggcct acaaggccgg agtcaggatc gcgttcggca
     3121 ccgacgctgg cgtgtacccg cacggcggga acgcgcagga attcgtgtac atggtgcagg
     3181 ccggcatgcc gccgatgtac gtgatccagg ccgcgaccac tcacgccgcg acgctgctga
     3241 agcaccagga tgatttcggc agtatcacca ccggcaagta cgcggacgtg gtggcggtgc
     3301 cggggaatcc gctggacgac atttcattga tgaagaaggt ggacttcgtg atgaaggcgg
     3361 gcaccgtgta caagcagcag ggcaagccga ccgacgcgga cctggagtcc agccttggaa
     3421 tgtaatgcca gcgcctccag ttatccggcc atgaacttca atcccaaacc cgatgatgcc
     3481 gcgcctgccg acagcttcgt gttcgaggcc ggcgaatcca atttcgaagc cgacgtcctg
     3541 caggcttccc tgcaaactcc ggtgctggtg gatttctggg cgacctggtg cgggccgtgc
     3601 aagacactcg ggccgatcct ggaaaaactg gccgatgaat acgcgggcgg ctttcgcctc
     3661 gccaagatcg actgcgacaa ggagcagcag ctcgccggga tgttcggcgt gcgcagcatc
     3721 ccgaccgtgg tgctgatcca gggtggccag atcgtggatg cgttctccgg tgcgttgccg
     3781 gaatcgcagg tgcgcgaatt cctgaagcgc catggcgtcg aacccgcctc ccgcatcgag
     3841 gcgccggcgc aggctgtcga agatggcgcc gcgatgccgg cggaaacccc gcaggccgct
     3901 atcgcacgga tcgagcaagc gctggcggcc gatcccgaca acgccggctt gaagctcgac
     3961 ctggcgctgg cgcgtgcgcg cggcggcgac accgccaacg cacagtccac actggatacc
     4021 ctgcccgtcg atctggccga agacgatcgc gccaaggccc tggcggcgtt gcttgcgatg
     4081 catcagtcgc tcgccgcgat tccgcctgcc ggcgaattgc tggcgcgcgt ggagcgcgat
     4141 ccccgtgact ttgcggcgct ggatggcctg ggcgtgcgcc agttgctggg cggagatgcg
     4201 gccgacgcca tgcagcgctg gctggcgatc ctcgctgccg atcgaagctg gaatgacgga
     4261 ctggcccgca agcgactcgt tgatgcgttc cggatcgtgc acgacgaagc gctggtttcg
     4321 accacccgcc gcaagatgtc gtcattgctc ttctgacacc gcggatgcgc gcgcggaccc
     4381 tgcggcggtt gatgaacctg tggccgccgt tcctcgcgaa cggcatccgg gtcctcgaga
     4441 tcagcgacga ctggcgccgc gcccgggtcg cgctcaagct gcgtccctgg aaccggaacc
     4501 atgtcggcag tcagttcggc ggcaacctgt tcgcgatgac cgatccattc tggatggtgc
     4561 tggcgctgca tcgcctgggc ccggactatt atgtgtggga caaggccgcc agcatcgagt
     4621 tcgtggcgcc tgcacgcgaa gacgtgtttg ccgggttcca cctcgatgac gacgccgtgg
     4681 cgctgcttcg ccacgaagcc gcatcgggcg acaaggtgct gcactggttc gacaccgaag
     4741 tcgccaccgg ctccggcgaa acgatcgcac gggtgcgaaa gcagctctac gtccggctga
     4801 agccgcgggc acgcagcgcg cgcccgtcct gaccggcttg ggcactgcgg gcagggcacg
     4861 cggcgactgg catttttcgt gcatgcgggg aaccggaggt gcgcccgggc attgaccgaa
     4921 gcagtgggat ggcaacgccc gtgacggggc gcgcatggat cgccgtccac ggctagcgta
     4981 cagtgtcagc ggaaagatga acgacgccac gccacaccag ctcgaagttc cgggataccg
     5041 gttggtccgg ccgatcggca ccagcggcgt ggccagcgtg tacgaagcga cgcagcgctc
     5101 gctcgagcgc caggtggcga tcaaactgtg gcggccgctg gacgacaacg cgctcggtcg
     5161 cttcgaacaa ctgctccgga ccaacgcgcg gctgtcgcac cccaacatcg tcggcatcca
     5221 ccagatcggc cgcaccgagg acgggcggct gttccactcg atgccgttcc tgttgagcat
     5281 cggtctttcc gcgcacaacc tgcgcttcaa gccgttgaag gtcgccggac tgctgcgcgc
     5341 gctgctcgac gcgctcggcc acgcgcatcg ccgcggcatc gtgcacggcg gcatcaaggc
     5401 ttccaacgtg ctgatggatc agcgcggcca cgtacgcctg gccgatttcg gcattgcgcg
     5461 ctgcgcagcc gaactcggtc ttcggcacgc atccgcggcg gcctacctga gccccgaaca
     5521 ggcgcgcggc gaaccgccgg gccagcgctc cgacctgtac agcatcggca tcctggcgtt
     5581 cgaattgctg accggcgcac caccgttcca gggcggcgat gcggtgtcga ccgccatcgc
     5641 acacgtcgag cagccgatac cgaggttgcc accgctggtc ggcggctggc aggcatggat
     5701 cgacaaggcg ctcgccaagt cacccgaaca gcgtttccag ggcgcccagg aaatggcgga
     5761 cgcgttgtcc gcgatcgatg ggcgccgcag gatccggccg gtggcggtgc cggtcggctc
     5821 gcggctgcgc gcccccacct gggcgatcgc cggggcagcg gtgggcgcgg cgctggtatt
     5881 ggcggtaggt gcgtgggcaa cctggcgctc ttttccgcgc aacgatgccg cgaccctgcc
     5941 cggacccgtg gtgatcgcgg cgaaagccgc gactgcgtcg ttgcctgcgt cggcgccccc
     6001 ggcggccagc accgcaccgt cgacccaaga ccgagcctac gcattggtca aggaggcaga
     6061 tgccttgcgt acccgcggcc gcctgttcgc gcctgccggc aacaacgccg ccgacaagta
     6121 cctcgccgct ttggccctcg accccggcaa cccggcggcg accgccggca tcgatgcaat
     6181 gctggcgacg ctgcgccaac gcctcgctgc cgcgtggagc gacggcaacc tggaccggac
     6241 ggccgcgatg ttgaagcgtt gcgacctgtt ggcgtcgcac gcgggtcccg tcgcacgccg
     6301 cgactggcgc accgaccgcg aggatttcgc ggagcacgtc ggcgatgcgg tggtcgccgc
     6361 ggccgacaag cgcgatattg tccggctcgc cgcactgaaa cccctggcgc aggcattgcc
     6421 cgcgacctac cctgccggat tcgacctggc cgtggccgaa cggcgggcgg ccaccccggt
     6481 cgccggcgac cggatgcacg atgagcgcgg tcccacgctc gtttacgtgc cggcgtccgg
     6541 cggggcgccg gcgttcgcga tcgagcgcgt ggaagtcacc cgcggcgact acgcggcgtt
     6601 cgtacgcgcc acccatcgtc ccgattcgcg ctgcctcgag gcctacaacc cgttctcgcg
     6661 gctgcgccat ctgagctggc aggatcccgg cttcaagcaa ggcgacgatc atcccgtcgt
     6721 gtgcgtgagc tggaacgatg ccgttgcgta cgcggcgtgg ctttcgaagg aaaccgggca
     6781 ggcctacatg ttgccgagca gcggcgaatg gctgcgcgcg gcgcaagggc tccccggggc
     6841 cacgccctgc cagctcggca acgtcgacga caccagccgc caaagcgcga tggacaacga
     6901 tcgcctgtcc tgcaacgacg gcgccgcgcg gacggcgccg gtcgggcact acctgccgag
     6961 cggtgcgggc gtgtacgacc tgtacggcaa tgtcagcgaa tgggtggccg acggcagcag
     7021 cggagagcgg gtgttccgag gtctttcctg gcgcgatggc ggccagggca agccgctcgc
     7081 acgccgcggc accgccgaca gcgacgtcgg ttacaccaat gtcggattcc gcgtcgtgcg
     7141 cgtgatcgac gacgcgcacc ccgcgccccc agtggcacgc gcaagccgat aagcaagtca
     7201 cgtcggaggc tcttgcaggt tggggccggg cgcagcgaag cccaacaagc ccagtccatc
     7261 gtgttcgcca tcctcgtcgt gttcgcccga gattgcggtt gggcctctcc cggagcaagt
     7321 ccgggatcaa cccaacctac gtcagccgct tctgtaggtt ggcgccgagc gcagcgaagc
     7381 gccacaaacc cagtccatcg ttcgccaccc cgtcgtgttt tcgcccgaca ttgccgttgg
     7441 cagccgcttc tgtaggttgg cgctgagcgc agcgaagccc aacaatcgcg ccgcccagcg
     7501 cgctcgccgc cctcgttgtg tcctcgcccg acattgccgt tgggcttctc ccgaacaggt
     7561 ccgggatcaa cccaaccccg cgaagcggct gcgtgttccg gcatccgcaa gcatgacttg
     7621 tggcgtatga cgatgggtac gccggcgcgc atactcgggc gtccccgaac gcctgcgcgg
     7681 tcgggttccc ttcgacaact cgatcagatc agccgggagc gtccatgaaa gccaaccgtc
     7741 tcgccgttgc cctcgcctcg ctgctgctgt gcagcgcccc cgcattcgcc gcgcctccgg
     7801 ccagtgccag cagttcggcg caaagcgact ccagcggcgg gcagcatgaa agcctgatca
     7861 agcgcctgtc gcaaccgatg tcgtccacga cccacggcac cgtcaccgtc gaaggcaaga
     7921 cgatcagcta cgaagcggtg gccggaacgc tggtgctcga cggcaccggc gcgaaggagt
     7981 ccacgcccga agtggcgatg agctactttg cctacttcaa gcagggcgtc gatccgtcca
     8041 accgtccgat caccttcatc tacaacggcg gccccggttc gtcgacgatg tggctgcaca
     8101 tgggcgcctg gggtccgaag cgcgtcgtca ccaacgacca cacccacacc cctgcggcgc
     8161 cgtatcaact ggtcaacaac gactacagcc tgctggatgc cagcgacctg gtgttcatcg
     8221 acatgccggg caccggcttc ggccgcctgt tgccgcaggg caaggacgac gacgcccgcg
     8281 ccaaggatcg caaggacctc gccaagcaga tctggggcat cgacggcgac gccgactcgt
     8341 tcgcgcactt catcacccag ttcctgacca agtacgaccg ctggaattcg ccgaaatacc
     8401 tcttcggcga aagctacggc accacgcgct cggcggtgct ctcgaacatc ctgcagaccg
     8461 aatacaacgt cgacttgaac ggcgtgatgc tgctgtcgca gatcctcaac ttcgggctca
     8521 gcatcgacgg cgccgacatg aaccccggca tcgacatgtc gtatgtgctc ggcctgccga
     8581 ccttcgccgc gacggcgtgg taccaccaca agctgccgaa gttcaacaat gggagcctgg
     8641 caccgctcct caaggaagtg acccagttcg cgacgacgga ctacgagcag gcgctgtcgg
     8701 ccggctccac cctgagcgcc gcgaagaaac agcagatcgc cgggaagctg cacgactata
     8761 ccgggctccc ggttgattac ctgctgaagg ccgacctccg cgtgaccggc ggcatgttcg
     8821 agcacgagct gctcgacgac tccgacacca caaccggccg gctcgacacc cgcttctcgg
     8881 gcgcgaccat ggacccgctc ggcaaggagt cggattacga tccgcaatcg gcggcgatca
     8941 gcgccgccta cgtggccgcg ttcaacagct acgtgcgcaa cgacctgaag ttcggcgaag
     9001 gcatgaagta ccgtccctcc gtgtacggtg ccgatgactt ccgctgggac tggaagcacc
     9061 gcgcgccggg ccagcggtat tcgcgcggcg gcacgccgaa cgtgatgctc gacctcgccg
     9121 tggcgatgaa gtacaacccg gacatgaagg tccggctgtt cggcggcttc tacgaccttg
     9181 cgacgccgta ctacaccgcc atctacgaaa tgcagcacct gccgatcccc gagaagctgc
     9241 agaagaacat cagctacggc ttcttcccgt ccggccacat ggtgtacgcg catacgccgt
     9301 cgctgaagaa gctgcacgac gacgccgtgc agttcatcga cgcgagcgac aatctcggga
     9361 acaagtaggc ccggcgctgc ccgccatcga tcgctcggcg gacgatgtcc gcctgcggat
     9421 ccatcaagcc ggcgcgacgc aagtcgcgcc ggctttttca ttcctgttcg aagcgcgcga
     9481 tttcatccag cgcgtcctcc gccgcgagca cgctgctccc cgccgcatgg ttttccaacc
     9541 ggcgaccgta accgagccgg agtgccagcc atatgcccat ggctaccagg cccaggctgg
     9601 ccgcgccgct gagtaccgcc caccgcccca agccgggcgc ccacggatcg aatccggacg
     9661 acgcgaacgc catcacgaat gcagggatcc agatgaagct gcccacgatg gcgtcgatcg
     9721 gtgcctcgac cttcacgcgc catttgcgca gttgcgccaa ttgatgctgg atgacggtca
     9781 ccggcgcacc atggtccacg cggcgcacca attccagtac ccgcgcggcc gacaggatca
     9841 tcagcagccc gaagccctgc atcagcaagc cgcaaaccag caggtgcagc acctgcctgt
     9901 gcgacaccca gaaggttacc gcccaggttg ccagcgctac gccgatcaac aactgcaggg
     9961 actgtcccca caccagtggt cgcagcccgc gacgcagttt gtcgagcccc tgattccgga
    10021 tcaatcgcag gttcaacgtg cgctgctgct ccagtttcgc ccccagctcc tgccacgcca
    10081 acctcatttc atcgagttcc atagtccact tcctcggttg cagctgccgt tgcctgcggg
    10141 cgcagcttct gcctgatgcg gttgagtttg gtcgcgacgt tggtcacgct tatcccgagc
    10201 acctccgcga tttccgcgta actgcgatcc tcgagataaa gcagcaccag cgcgcggttg
    10261 agcggatcca gcccgtcgat gaacgcgtac agcgcggcaa tgcgctccgc acgttccagg
    10321 ccatcggcgg gcgcctcgtc ggtggacgcg acggacgcga catgcggctc ttcgaagggt
    10381 tcggcgtgtc cggcacccgc gcgatgggcg tgccgcgcat gcgagatcgc cacgttcaag
    10441 gcaacccgat acatccatgt cgagaacttc gcgcgccgct cgtcgaagcg cccgaacgat
    10501 cgccagagtt gcgcggcgat ttcctgggcg agatcgtcgc gatcgggcgc accggtcgcg
    10561 tacgcagccg cgatcttgaa cacgatccgc tggtgttcgc gcaacagtac ttcgaatcgc
    10621 cgttgccgct gctggtccgc catcgtccga ttccgccccg gctccgatca ccgtttcccc
    10681 atgattcgcg aacccgcgac aaagatcaca ccagcgggcg cgtacggctc cactacaatg
    10741 cccgaaccgt ggcgtacccg tcttgtcgat gcccgccctc cacgtcaagc gctacctcat
    10801 cacgggcctc ctggccttca tcccggtgtg gatcacctgg gtggtgttcg aattcgtgtt
    10861 caccctgctc gcgggcgttg gggcaccgct ggtgaacggg atcatcgggc tgattggcct
    10921 cggcgcgccg gtggcggcgc aggtactgca ccgcgactgg gtggtatcga tcctcgcctt
    10981 cgtcctcacc ctgctcgcgt tgtacgtgct gggctggctg tccaccaagg tgctcgggcg
    11041 ccgcgtgctc ggcgcggtcg atgccttgct ggaacgcatt ccactcgtgc acacgatcta
    11101 cggcggcgtc aagaaactga tgacgctgat gcgcaagaaa ccgggcggca cccagcgggt
    11161 ggtgctgatc gacttcccgt cgcccgagct gaagtcgatc ggcttcgtga cccgcacctt
    11221 cgtggacgcg gccgggtgcg aggtcgcggc ggtctacgtg ccgacgacac ccaaccccac
    11281 cggcggctat ctggaactcg tacccaccga acgcctcgtc gcgaccgact ggaccattga
    11341 ccaggcgatg gcgttcctgg tctccggtgg tgccgtcggt cccgaacaac tgcccgccgt
    11401 ttcgcctgcc ccggcgccgc ttcgcgcacc ggtggagacc accggggcat gagcgggcca
    11461 gccgctcccg ccgccaccgg cgaaaccgcc cgctgctttc tcgcgctgct tcccgattcc
    11521 gccagccgcg acgcgctgct gcgcttccga caggccctcg aagcctcgcg gagcggcgcc
    11581 gcgcgcggcg tccgctggct ggatgccgcg tcgctgcacg tgacgctgcg tttcctcggc
    11641 gattcgacac gtgcccaggt cgaattcctc aagcacatgc tggcggcact cgcgcggcaa
    11701 ctgccagcat tcgacgcgcg gcgctgcgcc atctggccca accgcgcgcg gccgcgcctg
    11761 ctggtgctgg aactcgacgc gcccggggag ctgcgcgcgc tggcggagga atccgaaggg
    11821 ctggcgcgca aggccggctt cgaagccgaa ccccgtgcat ttcgcgcgca cctgacgctg
    11881 gcgcgcctgc ggccgggttg taccctggag gtcccttcgg tgccgttgcc caggctcgca
    11941 ttcgaatcac tgtcgctgat gcagagcgag ctccaggcaa ccggcgcccg ctaccagccg
    12001 ctcgcgaccg ttccgctgtc cggttcgcgg aaatgttgat gcgaggcggc gcgaaacccg
    12061 ctacactgcg ccccgtttga cgcgccgtac cccgaacttc ccttgcggat cgtcgtgacc
    12121 gggccgcctc tttggccccg atccgatccc ccgccactcg cacgtcgcgc acacccggcc
    12181 tcgcatccgc gttcgccgca cacaagttgc aacactcgca gcgcggttct tgggaacgct
    12241 ccgggtcaca caggagtttc cccatgacgt tcgaatcgct cgggctggcg cccgcgctgc
    12301 tgcgtgccct cgccgaacag ggctacgcga atcccactcc gatccaggtc gcggcgatcc
    12361 cgctggtgct gaacgggcac gacctgatgg ccggcgcgca gacaggtacc ggcaagaccg
    12421 ccgcgttcgc cctgccgctg ttgcagaagc tgtgcgtgga ccaggccaac cgcgacagca
    12481 agcagccgcg caagccgcgc gcgctgatcc tcacccctac ccgcgagctc gccgcgcagg
    12541 tgcacgacag cgtgcgcagc tacggcaagc acctgcgtgt ctacagcgcc gcgatcttcg
    12601 gcggcgtcgg gatgggtgga caggtcatga gccttcgccg aagcctcgac atcatcgtgg
    12661 ccacgccggg tcggctcatc gatcacatgg accgccgcaa cgtcgacctg tcgtgcatcg
    12721 acacgctggt gctggacgag gccgaccgca tgctcgacat gggctttgcc cccgcgttga
    12781 agaaaattct cggcgttctg cccaagcaac aacgacagac gctgctgttc tcggccacct
    12841 tcgtaccgga gatcaaggcg ttggcggcgc aattcatgca cgacccgcgc gaggtgcaga
    12901 tggcgcagcc gaacgcggtg gcggtcggtg tccaccatcg cgtgcacccg gtggatgccg
    12961 agaagaaacg cgacttgctg ctgcacctgc tggccgccga cagccgccgg cagacactgg
    13021 ttttcgcgcg caccaagcac ggctccgaca agttgtgcaa atacctcgac cagcacggct
    13081 ttcgcgtcgc cgcgatccac ggcaacaaga gccagggcca gcgcacccgc gcactcgccg
    13141 atttcaagaa cggcaagacc accgtcctgg tcgccaccga catcgcggcg cgtggactgg
    13201 acatcaacca gttgccggcc gtcgtgaact acgacctgcc gatggtcgcg caggactacg
    13261 tgcaccggat cggccgcacc ggccgcgccg gcagcgaggg cctcgcgttg tcgctggtca
    13321 gccgcgagga ggaaggactg ctgcgtgaca tccgcaggct gctgaaacag gagcttgcga
    13381 tcgaacccgt ggagggtttc gaaccgacat ggcagccccg cggcgagcgc gacgatgtcc
    13441 ggtcggccaa ggcggggccg cgaccaggca aaccgcgcca tgcgcggcag gtccggcgcg
    13501 acggcatcgc caagcccgga tcgcgctgga aacaggcgtc cgggcgcacg cgcggcaagc
    13561 cgtcgaacac agcggcgcac gccgagggca acgtcgccaa gcgtggccgc ggcaaacgcc
    13621 ccgggtggca gtcgggttcg cgctgacgcc gccgctcgcc gccagccgcc tgcaccgcga
    13681 ggcgcgccgc cgcactgtac ggcgtgcgcg ccttcggtcg gtgcaataca ggcatgactt
    13741 ccgacatggt ggtgcgccat cgtgattcga tgctggcaca atcgccgcct caagcggtgc
    13801 gcgcggcgcg tctccgcaac cccgtcacgc actgacagag gacccatcat gcacaagcat
    13861 ctgctgaccc cgatcgcgct ggccatcggc gcggcgctgg ccgtggctgg ctgcagcaac
    13921 aacgcccaga accagaacgg cgtcgcgccc aacggcgcgg catccgcacc agtccccgcg
    13981 caaacggccg cggcaccagc ggcaggttcc acggtcgcga tcatgcccgc gcagccgatc
    14041 gcgttcgacc tcgacgacca gtacaacccc tgcaacgact tcgccgaata cgtcaatgcg
    14101 aagtggaaca aggccaatcc gatcccggcc gacgaaaccc gctggggcgc cttcggcatc
    14161 ctccacgaac gcagcctgca gcaacaaaag cagatcctcg aaacggccgc acaggatgcc
    14221 aaggacggca agggcaacga actccagcag aagctcggcc acctgtatgc cgcggggatg
    14281 gacaccgcca cgatcaacaa gctgggctac gacccgatca agccgcagct cgcggcgatc
    14341 gacgcgctca agaccccggc cgacatcgcc gacttcatca ccaccagcta cgacaacggc
    14401 gacagctatg tcttcaactt cggctcgagc gcggacttca aggacgcgac caaacaaatt
    14461 ggctacgtgt tccaggacgg ccttggcctg cccacccgcg actactacct gtcgccgaag
    14521 tacaaggaca tccgcgctgc ctacctgaaa tacatctcca aatcgctgca gctcgtcggc
    14581 accccggcgg ccgacgcgaa aaagcaggcg gacgaagtct tgaagttcga aaccgaactc
    14641 gccaaggctt cgctggctcc ggtcgaactg cgcgacccga aaaaccagta ccacttcgtc
    14701 accgtggcgg aggccgacaa ggtcagcccg cacttcagtt gggacaagtt cttcaagggc
    14761 cagggcgtgg acatcgggaa gggcttctcg ctgtcgcagc cgaagttcat cgccgagttc
    14821 gacaagctgc tcgcgcatgc gccggcatcg caatggcagg cctacctcaa attccacctg
    14881 atcagcagcg cgtcgccggc cctgagccag gcattccagg acaaccagtt cgacttctac
    14941 ggcaagaccc tgcagggcca gctcgagcag aaggaccggt ggaagcgggt gatcggcgcc
    15001 gtcaacgggg cgatgggcat gggcctgggc gagctttacg tcgccaagta cttcccgccc
    15061 gagtccaagc agcgtgcgga ggaactggtc accaatgtcc gcgcggcgct gaaggaacac
    15121 atcgagaacc tcgactggat gagcgaggcg accaagaaga aggcgctcga caagtggtcg
    15181 ctgttcctgc ccaagatcgg ctatcccgac aagggcgagt ggcgcgactg gtcggggctg
    15241 acgatccagc cggacaagtg gtacgccgac ctcgaggcgg cgtcgaagta caactaccac
    15301 tacgacatcg gcaagatcgg caaggcgacg gatcgcaagg aatggcacat gacgccgcag
    15361 acgatcaatg cctattactc gccttccacc aacaccatca acttcccggc ggcgatcctg
    15421 cagccgccgt tcttctacgc caagggcgac gacgcgctga actatggcgg catcggattc
    15481 gtgatcgggc acgaatccag ccacggcttc gacgacgaag gcagccagtt cgacggccac
    15541 ggcaatctcg tgaactggtg gacgccccag gacaagaagc agttcgatga gcgcaccgcg
    15601 gcgctggtca agcagtacga cagctacacg ccgatcgagg gcaagcctga cctgcacgtc
    15661 aacggcaagc tgacgctggg cgaggacatc ggcgacctgg gcgggctcaa catcgcctac
    15721 accgcattgc agaccgcgct gaaacagaac ccccaggaag cggaccagaa gatcgacggc
    15781 atgacccaga atcagcgctt cttcctcagt tccgcgcggg tttgggaagg cacttcccgc
    15841 gacaagacct cggaactgct gttgaatgtc gatccgcacg cccccgccaa gatccgcgcg
    15901 ttcgcgtcgg ccgccaacat gccgcaattc gcgcaggcct tccagtgcaa gcccggcgac
    15961 aagatgatgc atacgggcga caagctggtg aagatctggt aggagggatt cggacagcat
    16021 aaatcgcttc acgctcgaac ttcagacgcc gcacgcaagt gcggcgtctt tttgtgcgcg
    16081 agctggaagc gatcgccgga gcaattccgc tgcgtgtcca gctccgacat acatcagact
    16141 cgtattgcga atcgttcgca tttgccttag tatccgccgt gcttcccgcg cagttctcag
    16201 gcgcgggcca aaacatcggt cgccgatgtg cggcttcgcg ctcctgtgcc cacaccgggc
    16261 ctgtgcgatc gcccgcccgg cgcacaggaa caaggcccaa cctccatgcc aggcatcaag
    16321 caccgcaagc acgctgccgc ccgctgcaag cctccgctcg ccctctccac cctgatgacc
    16381 ggcatcgcgt tcgccctgcc ctcgctggcg gccagcggtc ccgcagcgcc ggcgggcttg
    16441 gccggggcgg cacagctacc gaccatccac gttcgaggaa cccgcaaccc ctacaagatc
    16501 gacgtggtgg attcgcccaa gttcacccag tcgctgctgg atacgccaca gacgatcaac
    16561 gtcatcggca aggaaatcat cgagcagcag ggcgccacga cgctcaccga ggcgctgcgc
    16621 aacagtcccg gggtcggcac gttctacgtc ggcgaaaatg gcagcacctc gaccggcgat
    16681 gcaatctaca tgcgcggttt cgatacctcc ggcagcatct tcgtggacgg cgtgcgcgac
    16741 ctcggatcga tctcgcgcga cgtgttcgac gtcgaacagg tggaagtcac caagggcccg
    16801 gacggaaccg aatacggccg caccgcgcca accggcgcga tcaacatggt cagcaagcag
    16861 ccgcgttcga aggaccaggt ggaggcgtcc gtcggctacg gcagcgccaa ccgtcgccgc
    16921 gccaccgcgg actggaacca ggtgatcgac cggcacacgg cattccgcct caacgtgatg
    16981 ggccagaaga gcggcgtcgc cggccgcgac gaggtgcggg aggaccgctg gggcatcgcg
    17041 ccttcgctgg cattcggtct gggcaccccc accactgtct tcgtcgattt cctgcacgtc
    17101 acgcaggaca acgtgccgga cggcggggtg ccgacgatcg ggctgccggg ttatgcgacg
    17161 cccgatccgt cgcgtccgtt cctggcggat gcgccgaagg tcgatccatc caacttctac
    17221 ggcaccgacg ccgaccacga cgatgtcgag gcggacatgt tcaccgtgat cgccagccac
    17281 gacttcgaca acggcgtggc gttgcacgac acgctccgct ggggccgcac ccacgaggat
    17341 tacctcctca cctccttcat gggccgtgcc gatcgcctcc tgactccgga tcccgccgat
    17401 cttgcgacct ggacgatcgc gcgcagcaat ccgaccttca aggaccagac caatcgcatc
    17461 ctcaccaacc agaccaacct caccgcgcat ctcggcagtg gagcggtcga gcacgacctc
    17521 agcgccggca tcgagctgac ccgcgaggag atccgcaccg gtggcaccgg ggccctggac
    17581 gacagtgcat ggacgccggc gaacctgtat cgcccggatc cgcacgctgc caccggcctc
    17641 gtcatcggcg acaccggcgc ctggagccgg gggcgcaccg acaccgccgc ggcgtatttc
    17701 ttcgacacca tgcacttcgg cgagcgttgg ctcgtcactg ccggtgcgcg cgtcgaccac
    17761 tacaccaccg ggttccacag cgtcgaaccc tgcgaaagct cgcgacgcgc cccgtcgtgc
    17821 aacgggagcc cggctggcac accgctgcca tcggtagacg cggacctcgg cggcaacctg
    17881 ttcggctaca agctcggcat cgtgtacaag ccggtggcgg acgggagcat ctatgcgaac
    17941 ttcgcggccg cggccgaacc ccccggcggc aacaacctgg agttgagttc gtcggccagc
    18001 agcgcggaca acccggcctt cgacccgcag cacgcccgca ccgtcgaagt cggcaccaag
    18061 tgggacgtgt tcgacggcca gttgcggctg accgccgcag cgtatcgaac cgtcgtgacc
    18121 aacctcgtcg aacaggatcc cgtcgatctc cagtactacc agatcggccg ccagcgcgtg
    18181 cagggcatcg agctcggcgc ggtcggacgg atctcgcaaa actgggcggt cagcgccggc
    18241 tacaccctga tggacgcggc ggtcacgcgc ggcgcggccg tggccgaaga tggatcggac
    18301 gcgttgccgt acacgccacg gagcgcattc acggcctggt cgtcgtggcg actgccccac
    18361 catgtcacgc tcgccgccgg cgcgcgctac tccggcgaaa tgcggcgggg caccgacggc
    18421 gccgtcggca cgcccgcctt caccgaggcc tactgggtgt tggatgcgat ggcgagttac
    18481 gccatcaacc ggcggatgcg gctgcagttg aacgtctaca atctgttcga caggcactac
    18541 gtcgccgcga tcaacaagag cggctaccgc tacacgcccg gcatgccgcg ttcggccttg
    18601 ctgacattga acatccatta ctgaccccca tgctgctgca catcgagcac gtcctggacg
    18661 ccgatacggt cacgcggatg cgcgcgcacc tcgacgcggc cgaatggacc gacggccgtg
    18721 aaaccgtggg gccgcaaggc gcgctcgtga agcgcaacct gcagttgccg gatgcatcgc
    18781 cgctgcgcgc gcaactcggc cgtgaagtcc tcgcggcctg cgcgcgtcac gccgacttcc
    18841 acgcggcaac gttgccggtg aagatcctgc cgccacgttt caaccgctac gccgacggcg
    18901 gcagctacgg cgcgcacgtc gatgggtcgg tgatggcgct cgccgacggc agccagttgc
    18961 gcagcgacat cgcctgcacg gtgttcctcg ccgagcccga tgattacgac ggcggcgaac
    19021 tggtcgtcga agacacctac ggcgagcatg aggtcaagct ggccgcgggc gacgccatcg
    19081 tgtacccggc gagcagcctc caccgcgtgg agccggtgac ccgcggtgcg cgcctcgcag
    19141 ccttcttctg ggtccagagc ctggtgcgcg acgaagcgca gcggcggacc ctgctgcaac
    19201 tggacagcgc cacccgccgg cttgccgccg ccgcggccga tgccgacgcg gtcaagcagc
    19261 tcgccggcgt ctatcacaac ctgttgaggc agtgggccga gacgtagtcg gggctgacgc
    19321 cggctccacc gggggaagcc caacttcggt ggtgcgattc atcgggcttc ttccgggcaa
    19381 ccccggaatc agcccaacct gcggcagagg ggccgtcgac gcgcgataat agccagcttt
    19441 ccgcactgcg aagcaacgat ggactacacg cccgggcaac gctggctttc ggaagcggag
    19501 cccgaactcg gcctcggcac cgtccttcgc accgacgagc gcgcggtgca ggtcctgttc
    19561 gcgaagtccg gagtggtgcg caactactcg gcacacggcg ccccgctgac gcgcgcgacg
    19621 ttccgggtcg ggcaacgggt agccggtcgc ggcgtctcct tcgtcgtcga acgcatcgat
    19681 ttgcgcgaag gtctcctcgt gtactcgggc gagaagcgcg aactcgggga aggccaactc
    19741 gacgacgaac agtcgctgtc gcacgcggac gcgcggttga tctcggcgcg gatcgacagg
    19801 ccgcaccact acgagctgcg caagcaggca ttgctggcac gcgccgccgc gcggcggtcc
    19861 gaggcctggg gcatcctgtc ggcccgggtt gatttgctgc cccatcagct cggggtggtg
    19921 gcgacctgca tggaacgcga gcatccgcgc gccctgctcg ccgacgaggc cgggctcggc
    19981 aaaaccatcg aagccggcat gctgatggcg cgcatgctcg cgaccggccg cgccgcgcgc
    20041 gtgttgatcc tgttgcccga cgcactggtc gtgcagtggt tcgtcgaact gcgccgccgc
    20101 ttccaactgg aattcgcgat cctcgacgag gaacgctgca atgccgtcgc caccgccgat
    20161 ccggccggca atccgttcga agccgagcaa ctcgtgatcg tgtcgctggc gtggctggcc
    20221 gccgatccgc agcgcaccgc gcaggcggtc gaggcaggct gggacctgct ggtggtggac
    20281 gaggcgcacc acctcgaatg gacaccggaa gcggcgagtc ccgaatacct cgccgtggaa
    20341 gcgctcgccg ccatctcacc gagcgtggtg ttgctgaccg cgacacccga gcagttggga
    20401 cggcgcgcgc acttcgcacg gctgcgcctg ctcgatccgg cgcgcttcga cgacctcgac
    20461 cgttacctcg gcgaagccga ccgctatgcg gcgctgtcgc ccgtcgcgga acgcattgcg
    20521 gccggcgatc cgctggacga ggctgcgcgc accgacctgt gcaatcggtt cccgcatgat
    20581 cccgccctgc acgcgctggt cgccgggtac ccagagtcgg cggaggaact gttgtcggca
    20641 ctgatcgacc gacacggcac cgggcgggtg atgtatcgca gccgccgcga aggcgtcggc
    20701 gggtttcccg cccgccaact cgagatcggt gacgcggcgg aactggcgcc cggggcgcgc
    20761 gccgacctgc tcgccgaatt cgaggacgac atgcacgcga cgccagtcgc ggcccagcgc
    20821 gcgctcgact attcgaacga cccgcggatc gcctggctgc tggccttgct cgaggggcac
    20881 cccgaagaca agttcctgct gctcgccagc acccgcgcca aggtggaggc gctcgacgcg
    20941 gccctgcgaa cgcgcaccgg aaccggtgtg gcgctgttcc acgaaggcct gacgttgttg
    21001 cagcgcgatc gcgccgccgc atggttcgcc cagcccgacg gcgcgcgcct gctgctgtgt
    21061 tcggagatcg gctccgaggg gcgcaacttc cagttcacgc accggctcgt cctgtgggac
    21121 ctgccgctcg accccgacct cgtcgagcag cggatcggcc gcctcgaccg catcggccag
    21181 cgccacgccg tggtgttgca cgtacccatg ctgccgggca ccgcgcagga ggcgctggag
    21241 cgctggcacg ccgatggcac ccgcgtcctg cgtgaatcgc ccgccgacgg acgcgagctg
    21301 ctgcgccgct tctgctgcga cgtggaggcg gtcgcgcgtg cgcatgcgga tggcggcgaa
    21361 tccgaggatc cggagctgga cagtctggtc gccacgaccg ccgccgtcca cgcggagctt
    21421 tcacagtcga tccgggacgg gcgcgaccgc ctgctggagc tagccacccg gcattccggt
    21481 ggtgccgcac tcctcgatgc gatgcgcaag gccgatcgcg acgagcagac ccacgccctg
    21541 atccagcaat gcttcgagcc gttcggcgtg cacgtcgagg acctcggcgg cgacgcgttc
    21601 ctgctggacc ccgaattcct gtccaccgat gccctgccgg ggttcgttga cggtccgcgc
    21661 cgggcgacgt tcgatcgtgt gctggcactc gcccgcgaag acctcgacct gccgcggatg
    21721 gaccaccccc tggtcctcgg cgcattcgag ctgctgctga ccggtgaatt cgggaatgcc
    21781 gcgttcctgg tcgatcccgc attgccgtcg cgcagcgtcc tgctggaggc gctgtacgtc
    21841 gtcgaatgcg tcgccgacgt gcggctggac gcgccgcggt tcctgccgcc gacaccgatc
    21901 agcgtctgcg tcgattcacg gctcgcttcg cgcgactaca cgccgtctcc gccgtcgctc
    21961 gcgcgggccc gcgaacgccc gctcgacatt tcgcgctacc ggcgctacct gtcgcaactg
    22021 gtgccaccga tgctcgacaa ggccaccgcg ctcgcccgcg aacgcgcggc cgaggtgacc
    22081 ggtgaagccg ccgcgcgggc cggtgccacg ctggatgcgc gggtggagcg gttgcgcgca
    22141 ctcgccgcga tcaacgccac ggtgcggccg gaggaaatcg cgggtgccga agccacgcgc
    22201 gaggccttgc tggccgccct cggtcgcgcg cgcgtacggc tcgacgcggt ccgcatggcc
    22261 gtcagccctg acttcctgac gctgcgctga gcggaggcgg attcgaagcg ccatgcgggc
    22321 ggcgcccgca atagggtcgc gccaagccgc tcgcgccgat caagcgctcc tgcaagaaac
    22381 ggtgcagccg cggtgaaaaa acgcaggagc gggccatgcc cgcgatccgg gctgcgcgcc
    22441 gatccgaccg cgcgccaccc cggtcgcgcc catgggacgc tgccacacca agggcatcgg
    22501 cgagcgctac accatcacgg ccggcgcgcg agttccgggt tttcgcgggt caccggcatc
    22561 aggtccttct tggtgacgcc gaaccacagc agcatggggc tcgcgaagaa gatcgacgac
    22621 agcgtgccca ccaggatgcc gatcagcatc gtgatcgcga atccgtgcac cgcggaaccg
    22681 ccgaacagcc acagcgtcgc catggtgatc gcggtgaaca gcgaggtgat gatcgtgcgc
    22741 gacaaggtgt tgttgatcgc gcggttgagg atctgcgggg tatcggcctt gcgggtgagc
    22801 cggaacaact cgcgcacgcg gtcgaacacc accaccttgt cgttgatgga atagccgatc
    22861 accgcaagga tcgaggccag ctccgtcatg tcgaactcgc gctgcaccaa cgcgatgatc
    22921 ccgaccgtga tgacggtgtc gtggatttca ctgaccaccg cggcgaacgc gaaccgtttt
    22981 tcgaatcggg cccacaggta gatcatgatc ccgatgatca cgactatggc cgcgtagatg
    23041 ccgctggtct tcaactcctg tcccacctcg ggaccgacga agtccggcgg gtgggcgagc
    23101 ttcacgcctg gatgcgcagc cgcgagcgcc gccagcacgc gcttggaaac ctccgccgcc
    23161 acctcgtccg ggctttgctt gtgcgccttg tccgcctgct tcggcggctg caggcggatc
    23221 gagatctcgc gggtgccgcc catgctctgc accacggcat
//
LOCUS       DASSNH010000115        17717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_112745, whole genome shotgun sequence.
ACCESSION   DASSNH010000115 DASSNH010000000
VERSION     DASSNH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 17717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17717
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112745"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFK00_04105"
     CDS             <1..1071
                     /locus_tag="VFK00_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET7592172.1"
                     /translation="LLFFACAAAAAQPPAAAQASSQATPASPSSIPTLQAINVTGVVP
                     GPGLWKVSRGDHVMWILGVVPTLPAGIEWRSAQVAQAIAGSQAVLKSPGVKLKVDTNW
                     FGKLFLLVPVYRAQRNPDGKTLHDVLPPAIYARWYYARERYFGNDYSVERYRPIIAAA
                     KLMQKALKANNLRGDGEIKDTVEALARQHGVALVDTRTTLEIRHPHDAVQAFVAAGPD
                     GIACLGLVLDMLDKRIPDVRARANAWATGDIATLRRVPESAYRDTCKSAITGAGFAKS
                     LGIENLPAQIEEHWLGAADAALAANTQSFAVLPMPALLDPGGFLAALQARGYTVTAPD
                     EAPVDEAAPAASVAPAASTSVH"
     gene            1085..1672
                     /locus_tag="VFK00_04110"
     CDS             1085..1672
                     /locus_tag="VFK00_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7592173.1"
                     /translation="MLASFNKPFDVLCQFTDRSVPPRRTLAEFGLPPDVYPAGRLDRD
                     SEGLLLLTDDGALAHRITDPRHASPKTYLVQVEGEPDDAALDKLRSGVVLKDGVTRPA
                     QARVLPYAPDWLWPREPPVRFRKSVPDAWLELTIHEGRNRQVRRMTAAVGLPTLRLVR
                     IRIGEHALDGLLPGRFRIHEPAPARANRSTRGARG"
     gene            1669..2412
                     /locus_tag="VFK00_04115"
     CDS             1669..2412
                     /locus_tag="VFK00_04115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7592174.1"
                     /translation="MSFKDHFSGHSNLYARARPRYPGALFAWLAEQAPAHGCAWDAGC
                     GNGQASVALARHFERVVATDPSDQQIGQAVPDPRVEYRAEPAEHTSLAAHSADAVTVA
                     QALHWFNLGAFVREVRRVARPGALFAAWSYANCSVAPAVDAIVADIYDRVLGPYWPPE
                     RRLVDEGYASLALPFVPVAAPAFEMHVDWSAAQFLAYLSSWSAAQRHRKATGVDAIAA
                     VADNVCAAWGDAAQPRPVRWKLAVRAGRV"
     gene            complement(2432..3223)
                     /locus_tag="VFK00_04120"
     CDS             complement(2432..3223)
                     /locus_tag="VFK00_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7592175.1"
                     /translation="MHLIDIGVNLTHESFRHDFDAVLERARGAGVDRMIVTGASRAGS
                     EHALELARAHPGTLRATAGVHPHHAGDYGEDTDALMRALARNPEVVAVGETGLDYFRD
                     LSPRPAQRAAFEKQLAIAIDLGKPLFLHQRDAHDDFFALLKSARDRVPAAVVHCFTDD
                     RRALFDYLDLDCHIGITGWICDERRGTHLRELVRSIPADRLLLETDAPYLLPRDIRPP
                     PTDRRNEPMYLAHICEAVARERGEAAETTAANATAATRAFFGLPM"
     gene            3338..6001
                     /gene="hrpB"
                     /locus_tag="VFK00_04125"
     CDS             3338..6001
                     /gene="hrpB"
                     /locus_tag="VFK00_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET7592176.1"
                     /translation="MSSDADVFPITPLLPAIRDSLRRHPRLVLEAPPGAGKTTQVPPA
                     LLEEPWLAGGTIVMLEPRRIAARAAAEFMAAQRGEAVGRTVGYRIRFESRVSDATRIL
                     VVTEGILTRMLQDDPGLDGIGAILFDEFHERHLPGDLGAALALEVQANLRPDLRLVAM
                     SATLEGERVAAWLAAPVLRGEGRAFPVRIEYPAPRREEAMESQVRRAVQQALGETDGD
                     VLVFLPGRREIARAQRMLVDGLLPSPPGKGDRGPSAQHCGEGARFERHPAEPEPSSGA
                     ARHLLPGREGLRQRHIEIVPLHGELGPAEQHAALAPSDPGTRRVILATNLAESSVTVP
                     GVRAVVDTGLAREPRFDPNSGFTRLATVNISRASADQRAGRAGRVAPGVAYRLWPESR
                     RLDSSRTPEIAQAELSGLALELAAWGVGADVEALSWLDPPPAGALAQARELLQGLGAL
                     ARDLRITGLGRDMLQVGTDPRMAAAALRAPESLRPLVADLLALLESRSPLPRFEDDLR
                     ARIDALHRFRDRGMRAGGHADSAALAAIEQTARGWRKRLGLRHGASGTPDALAVGNLL
                     LHAFPDRVARRDEKDPRRYRLANGRGARLREDSSLYGEPWLVIAEARFDARDSLVLAA
                     APFDPERLRLDHAARIAHERILRTNANDVLEAFEEARFGAIVLECRAVPVNPEDTLAA
                     LLATIRSRGIAALPWSDTALRLRARVGWLRQAMPDAGLPDVSDAALLDSLEAWLATAL
                     RGKRKLERLSAAELSNTFANLLDWSQRKLVDEAAPDALQVPSGMTRKLDYAAASRTAE
                     GIPSPVLAVKLQELFGLAETPRVARGRVPITLHLLSPAGRPIQVTQDLRGFWERTYPE
                     VKKELKGRYPKHPWPDDPWSAIPTHRAKRRS"
     gene            complement(6028..6747)
                     /locus_tag="VFK00_04130"
     CDS             complement(6028..6747)
                     /locus_tag="VFK00_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET7592177.1"
                     /translation="MTRIFAALIVATGLVIGGWFIGTGFAQSRQERFVTVKGLSEHDV
                     EADIALWRVEFVETGNQLADVQASIQRDAAAVTAFLSRHKLGDDAIAFRDVQVADRAA
                     RMYGNDQYTTRFVVTGTIMVRSRDVRAVAAAAQDTGALIAQGIVLGNADGGGGTGPNY
                     LFDGINALKPSMIAEATKNARTAAGQFARDSGSELGGIRRANQGVFEILPRDKAPMLE
                     ADRQIEKTVRVVATLEYTLKD"
     gene            complement(6835..8322)
                     /locus_tag="VFK00_04135"
     CDS             complement(6835..8322)
                     /locus_tag="VFK00_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET7592178.1"
                     /translation="MLRTRLLTALAAGALLTATAWAAAPPANHHPELRSIAAAVKASN
                     LHATDVALVGFGTRHTLSTTTSKTRGIGAARRWVAAQFQDISRGCNGCIEVVKPAQSF
                     TGPRMPKDGAKVMDIVAIQRGTTDPDRVIVMTAHLDSRRSDVMDATGDSPGANDDAAG
                     VAALIETARVLSKYKFPATLVYSADSGEEQGLYGGKIIAQYAVDHGWKVEANLNNDMV
                     GNSHGGNGAHDPHTIRVFSEGTKSNETLEQAAYRRYHGGGVDSPSRNIARYMAALAEQ
                     YLDDFRVRMVYRTDRWGRSGDQVPFLEQGFPAVRLSESNENYDHQHQNVRVENGVHYG
                     DLVSGVDFDYLANVTRLDAITMASLAMAPPPPADLTVKGAVAYDTTLSWQPSPGAASY
                     NAWWRETTAPQWQHLQKAGDADSVVLKDIILDDYFFGVSAVSKDGWESPAEFPGFSGS
                     FARSPKQGPDGNPVDQRYPGASEKYGGHAVGPASWQQDGGQHRKP"
     gene            complement(8352..9971)
                     /locus_tag="VFK00_04140"
     CDS             complement(8352..9971)
                     /locus_tag="VFK00_04140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7592179.1"
                     /translation="MNGTAINPQRIGSVGGWRWLALWFVLLLPLAILFPPIPIDETRY
                     LTAAWEMFNSGQWLVPTVNGAWYSDKSPLLFWLIAGGWKIFGVHTWVARVEALIVALC
                     MLLALRRLAARLGMTARGAADAMWILAGCLGFTVFATAIMFDLLLSLCVLGALHALLD
                     LDEGKWTRGVVALGITVGLGILAKGPVMLLHIVSVALLAPLWSGTARAHKLRWYLALL
                     AGLAIGIAIMLAWLLPAAAYAGPTYWQPLVDKVTGRIANSFAHRRPWWWYLPLVPVLL
                     LPWVLTLRAPRTAWAALWRGRFGRFLWSWWVVPFVAFCAISGKQVHYLLPLLPAWGVA
                     GAWLLQQPGGRLRSLSFGLLALLAAALVAVAPWHAAKLFGYPPQPAIALFALVGLVVA
                     AGAWRGWARDARGMALSATALVSAGMLAAALAYLPALDVRPEAAFVRNALLADSAIAF
                     VQPGEGLFGYAGRLHRPVPSVPLDGVQSWCKAHPGGILLTRERHEEPAAGTPFMAWPY
                     LLSGSRRIAAWRMPDDAARCVSVRAFVPPAG"
     gene            complement(10050..10631)
                     /locus_tag="VFK00_04145"
     CDS             complement(10050..10631)
                     /locus_tag="VFK00_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7592180.1"
                     /translation="MAATKPFLSDIETIRQRAREHIEKGAVTHGYTADRDTVIKVLNE
                     ALATELVCVLRYKYHYYMASGIHAQSVAAEFLEHAGEEQQHADKIAERITQLDGAPNL
                     NPEGLTSRAHSDYVEGDDLVDMIKEDLVAERIAIDSYREIIQYLGNDDPTTRRIMEEV
                     LAQEEEHAEDMKTLLEELGKEGEPPKPRDLKRK"
     gene            complement(10708..12585)
                     /gene="thiC"
                     /locus_tag="VFK00_04150"
     CDS             complement(10708..12585)
                     /gene="thiC"
                     /locus_tag="VFK00_04150"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET7592181.1"
                     /translation="MNAQPSDLLREAQQLSATVTSPIRGSRKTYATGSRDDIRVPMRE
                     VVLTETPAMFGVEANAPFTVYDTSGPYTDPAFKADLVAGLPPLRAAWIEERADTEVLP
                     NRTSKFGRRHAEAALLADVRFPNLPKPRRAKAGANVTQMHYARQGIVTPEMEFIAIRE
                     NQKLDALRGSALAQQHPGQSFGAAIPREITPEFVRDEVARGRAIIPCNVNHPETEPMI
                     IGRNFLTKINANIGTSAVTSSIAEEVEKMVWAIRWGGDTVMDLSTGKYIHETREWIMR
                     NSPVPIGTVPIYQALEKVGGAAEELTWELFRDTLIEQAEQGVDYFTIHAGVLLRFVPL
                     TAKRVTGIVSRGGSIMAKWCLAHHRESFLYTHFEEMCEIMKAYDVAFSLGDGLRPGCI
                     ADANDEAQFAELDTLGELTHIAWKHDVQVMIEGPGHVPMHLIKENMERQLDKCHEAPF
                     YTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKEDVREGIVAY
                     KIAAHAADLGKGHPGAQARDNALSKARFEFRWEDQFNLGLDPEKAKEFHDETLPKDAA
                     KTAHFCSMCGPKFCSMKITQDVRDYAREHGVDETEALDEGMAEKAAEFREHGGEVYRS
                     A"
     regulatory      complement(12705..12808)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13147..13782)
                     /locus_tag="VFK00_04155"
     CDS             complement(13147..13782)
                     /locus_tag="VFK00_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7592182.1"
                     /translation="MNASPERCAGPGRPKDLEKRAAILEAAKRLFPQHGFEGTSMDAV
                     ASEAGVSKLTVYSHFGGKEALFVETVRCKCEGMLPGLIFEVDVHAPVRSQLLGIARAF
                     FDLMMSDGALGMHRTLVASSQQSPKLAKLFWEAGPMQVQAGLASMLQKQVDARQLDIA
                     DTRRAACQFFALLKGEHHARMCFGCGKPDHEEAEAHLEATVDMFLRAYGRR"
     gene            14134..14799
                     /locus_tag="VFK00_04160"
     CDS             14134..14799
                     /locus_tag="VFK00_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HET7592183.1"
                     /translation="MNLNPPSEVARRNMVENQVRAWEVLDARVLDVLVALPREAFVPK
                     PYEALAYADVTLPIGYDEVMLKPVVEGRSLQALLPLASERVLEIGTGTGYFAACLSRL
                     AAHVTSIDIRADFVEAARQRLLDLAIDNVECVHADAFSGFAPDAPFDAIAVTGAVAEL
                     PPIFGEWLEPDGRMFVFQGFAPAMHAVLLERAADGGWIEDRRFETDVPYLTGAGPRPQ
                     FHL"
     gene            14906..16318
                     /locus_tag="VFK00_04165"
     CDS             14906..16318
                     /locus_tag="VFK00_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HET7592184.1"
                     /translation="MTQSPVSRHRFVPRALAIALVAALGLAGTATAHAENLMDAYRQA
                     LQSDPVLQQAEAQSRIGEAGADISRSALLPQLNGQVSFNDSHGISSGTQVFYDQNGNP
                     TLEDTTVHTGGRSRTESVGLNQVLFDLGQFSTWRASKASARAAEAQYTAAQQDLILRV
                     AQAYFTVLSDEEQLKYAEANEKALQKQLDQAQAKYDVGFAAITDVVDAKAQHDTAVAN
                     VINVKNQVFNDRQALAQITGQPPGTLETLTDKLPLAPPQPDDMQEWVNTALASNPSLQ
                     AQREQKEAAQHDVTAAYAGHLPTLDASVSYSRNPSWGGVGFTSSGAPRGDSRSSGTTV
                     GLVLTVPLFSGGGIHARVKQAIAQRDQAGDVLEQDRRTVVSNTRSAFNAIEAGISSVE
                     AQKAAVESAKTALDASEAGYQIGTRTIVDLLLAQQTYFQAQSAYSQARHALVVNRLNL
                     DYAAGTLSVDDLEAVNRLLQ"
     gene            complement(16373..17668)
                     /gene="waaA"
                     /locus_tag="VFK00_04170"
     CDS             complement(16373..17668)
                     /gene="waaA"
                     /locus_tag="VFK00_04170"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HET7592185.1"
                     /translation="MLRFLYTLAMYLVTPVIVARLVVHGVRYRSYLGRWRERFGRFPD
                     PGLHDCIWVHAVSVGEVNAAIPLIHSLRKRYAPRPMVVTTVTPTGSERVHNLFGDEVF
                     HVYLPYDLPAAVSRFLDRIRPALAVVMETEIWPNLFHQCGRRGIPLAVVNARLSERSL
                     RRYRPIRALVRQALGNVALVAAQSRADAVRYRTLGAQPSRVHVSGNLKYDMPLPKGAR
                     QHGEDLRDKWGDMRPVWIAASTHEGEELAAFKAHLTVLARMPDALLLVAPRHPERFRM
                     VEHAARNLGFKVATHSVDDVDEETQCLVIDAMGVMMRYFAAADLAFVGGSLVSIGGHN
                     VLEPAALSKPVVVGPNTFNFEEITRTLIEAGGARRVAAAAELGPMVLDLLRNGDELAR
                     MGAAARAVCARERGAARRTMVWLGRIVARARYASSARTD"
BASE COUNT         2803 a         5977 c         6096 g         2841 t
ORIGIN      
        1 ctgttgttct tcgcctgcgc tgccgcggcg gcgcaaccac cggccgcagc gcaggcgtcc
       61 agccaggcga cgcccgcttc cccgtcctcg atccccaccc tacaggccat caacgtcacc
      121 ggcgtggtgc cggggccggg actctggaag gtgagccgcg gggaccacgt gatgtggatc
      181 ctcggcgtgg tgccgacgct ccccgcgggc atcgaatggc gctccgcgca ggtcgcgcag
      241 gcgatcgccg gctcgcaggc ggtgctgaag tcgccgggcg tgaagctcaa ggtcgacacc
      301 aactggttcg gcaagctgtt cctgctggtg ccggtgtatc gcgcccagcg caatcccgac
      361 ggcaagacct tgcacgacgt cctgcccccc gcgatctacg cacgctggta ttacgcgcgt
      421 gaacgctatt tcgggaacga ttattcggtg gagcgttatc gcccgatcat cgcggccgcc
      481 aaattgatgc agaaggcgtt gaaggcgaac aacctgcgcg gcgacggcga aatcaaggac
      541 accgtggaag ccttggccag gcagcacggc gtcgcgctgg tcgacacgcg taccacgctg
      601 gagatccgcc atccgcacga tgcggtccag gcgttcgtcg cggccgggcc ggacggcatc
      661 gcgtgtctcg gacttgtgct ggacatgctg gacaagcgga tcccggacgt gcgcgcccgc
      721 gccaatgcgt gggcaacggg cgatattgcg accctgcgcc gggttccgga gagcgcctac
      781 cgcgacacct gcaagtcggc gatcaccggc gcgggcttcg cgaaatcgct cggcatcgag
      841 aacctgcccg cgcagatcga agagcactgg ctcggcgccg cagacgccgc actcgccgcg
      901 aacacgcaaa gcttcgcggt gttgccgatg ccggccctgc tcgatcccgg cgggttcctc
      961 gcggcgctgc aggcgcgggg ttacacggtc acggcgcccg acgaggcgcc cgtggacgaa
     1021 gcggcaccgg ccgcgagcgt cgcgcccgcc gcctcgacat cggtgcattg agcgaccgcc
     1081 tcgaatgctc gcgtccttca acaagccgtt cgacgtactg tgccagttca ccgatcgcag
     1141 cgtgccgccg cggcggacgc tggcggagtt cgggctgcca ccggacgtgt accccgcggg
     1201 gcggctggac cgggattcgg aaggcttgtt gctgcttacc gacgacggtg cgctggccca
     1261 ccggatcacc gatccgcggc acgcatcgcc caagacctac ctggtgcagg tcgaaggcga
     1321 gccggacgat gcagcgttgg acaagctgcg ctcgggcgtg gtgttgaagg acggcgtgac
     1381 gcgcccggcg caggcgcgcg tgctgccgta cgcgccggac tggctttggc cacgcgaacc
     1441 gccggtgcgg ttccgcaaat cggttcccga tgcctggctc gaattgacga tccatgaagg
     1501 ccgcaatagg caggtccgcc ggatgaccgc ggcggtggga ctgcccacgc tgcggttggt
     1561 ccgcatccgg atcggagaac acgcactgga tggcctgctg ccggggagat tccgcatcca
     1621 tgaaccggcg ccggcgcggg cgaaccggtc cacgaggggc gcgcggggat gagcttcaag
     1681 gatcatttct ccggccattc gaacctgtac gcgcgggcgc gcccccgcta tcccggtgcg
     1741 ctgttcgcat ggctcgccga gcaggcaccc gcgcacggat gcgcctggga tgcgggctgc
     1801 ggcaacgggc aggcgagtgt cgcgctggcc cggcatttcg agcgcgtggt cgccactgat
     1861 ccgagcgacc agcagatcgg gcaggccgtc cccgatccgc gcgtggaata ccgcgcggaa
     1921 cccgctgaac acacttctct cgccgcgcac tcggccgatg cggtgaccgt ggcgcaggcg
     1981 ctgcattggt tcaacctggg tgcgttcgta cgcgaggtgc ggcgcgtggc caggcccggt
     2041 gcgctgttcg cggcgtggtc gtatgcgaat tgcagcgtgg cgcccgcggt cgatgcgatc
     2101 gtcgcggaca tttacgatcg cgtgctgggg ccatactggc ctccggagcg gaggctggtc
     2161 gacgaaggtt acgcgtcgct ggcgctgccg ttcgttccgg tcgcggcccc cgccttcgag
     2221 atgcacgtgg actggagcgc agcgcagttc ctcgcctacc tctcgtcatg gtccgccgcg
     2281 caacgccacc gcaaggcgac cggcgtcgat gccattgccg cggtcgccga caacgtttgt
     2341 gcagcgtggg gcgatgcagc gcaaccgcgg cccgtacgct ggaaactggc ggtgcgggcg
     2401 ggacgcgtgt aggagcggcc atgggccgtt cctacatcgg caggccgaaa aacgcgcggg
     2461 ttgcggccgt cgcattcgcc gcggtcgtct cggccgcttc gccgcgctcg cgggcgaccg
     2521 cttcgcagat gtgcgcgagg tacatcggtt cgttgcgacg atcggtgggc ggtggccgga
     2581 tgtcgcgcgg caacaggtag ggtgcatcgg tttccagcag caggcgatcg gcaggaatcg
     2641 agcgcaccag ttcgcgcagg tgggtgccgc ggcgctcgtc gcagatccag ccggtgatcc
     2701 cgatgtggca atcgaggtcg aggtagtcga acagtgcacg tcgatcgtcg gtgaaacagt
     2761 ggaccacggc cgccggcacg cggtcccgtg cagacttcag caacgcgaaa aagtcatcgt
     2821 gcgcatcgcg ctggtgcagg aacagcggct tgccgaggtc gatcgcgatc gcgagctgct
     2881 tctcgaaggc ggcgcgctgc gcgggacggg gcgaaaggtc ccggaaatag tcgagcccgg
     2941 tttcgccgac cgcgaccacc tccggattgc gcgcgagcgc gcgcatcagc gcatcggtgt
     3001 cctcgccata gtcgccggcg tggtgcgggt gcacgcccgc ggtcgcgcgc agcgtgccgg
     3061 gatgtgcgcg cgcaagctcc agcgcatgtt cgctgccggc gcgtgacgcg ccggtcacga
     3121 tcatccggtc cacgcccgcc ccgcgggcac gctccagcac cgcatcgaaa tcgtggcgga
     3181 aggattcgtg ggtgaggttg acgccgatgt cgatcaggtg catgggtctg aagaagcggg
     3241 aacagggaac agggaacggc ggatgacgag ctgggcagcg taacaaaccg gggtgggcga
     3301 cgggaaagcg gccggtgccg acgcctacaa tgtggcgatg tcatcggacg cagatgtatt
     3361 cccgatcacg ccgctgctac cggcgatccg cgactcgctg cgcaggcatc cgcggctggt
     3421 gcttgaagcg ccgccgggcg cggggaagac cacgcaggtg ccgcccgcac tgctggagga
     3481 gccatggctc gccggcggca cgatcgtgat gctggagccg cgccggatcg cggcgcgcgc
     3541 cgcggcggaa ttcatggcgg cgcagcgcgg cgaagcggtg ggtcgcaccg tcggttatcg
     3601 gatccgcttc gagtcgcgcg tgtcggatgc aacgcgcatc ctggtggtca ccgaaggcat
     3661 cctgacgcgc atgctgcagg acgacccggg gctcgacggc attggcgcga tcctgttcga
     3721 cgagttccac gagcgccacc tccccggcga tctgggcgcg gcgctggcgc tggaggtgca
     3781 ggcgaatttg cgacccgacc ttcgtctcgt ggcgatgtcg gcgacactgg agggtgagcg
     3841 cgtggcggcg tggctggcgg cgcccgtgct gcgcggcgaa ggccgtgcgt ttccggtgcg
     3901 aatcgaatat ccggcgccgc gtcgcgagga ggccatggaa tcgcaggtac gccgcgcggt
     3961 gcagcaggcg ctcggggaaa ccgatggcga cgtgctggtg ttcctcccgg gtcggcgcga
     4021 gatcgcacgg gcgcaaagga tgctcgtcga tggactgctc ccttctcccc ccgggaaagg
     4081 cgaccgtgga ccgagtgcgc agcactgcgg ggaaggcgcg cgcttcgaac gacacccggc
     4141 ggaacccgaa ccctcatccg gcgctgcgcg ccaccttctc ccggggcgag aaggattgcg
     4201 gcaacggcac atcgaaatcg tgccgttgca cggcgagctc ggaccggccg agcaacacgc
     4261 tgcactggca ccctccgatc caggcacccg ccgggtgatc ctcgccacca atctcgcgga
     4321 atcgtcggtg accgtgccgg gggtgcgtgc ggtggtcgat acggggctgg cgcgggagcc
     4381 gcgcttcgat ccgaactcag gtttcacccg gctcgccacc gtcaacattt cgcgggcttc
     4441 cgccgaccag cgcgccggac gcgcggggcg cgttgcgccg ggggtcgcct atcgcctgtg
     4501 gccggaaagc cggcggctcg attcgtcacg tacaccggaa atcgcccagg ccgaattgtc
     4561 cggcctcgcg ctcgaactcg cggcgtgggg tgtcggtgcc gacgtcgagg cgctttcgtg
     4621 gctggacccg ccgcctgcgg gcgcgctcgc gcaagcgcgc gaactcctgc agggactcgg
     4681 tgcgctggcc agggacctgc gcatcaccgg actgggccgc gacatgctgc aggtcggcac
     4741 ggatccacgg atggccgctg cggcgttgcg cgcgccggaa tctttgcggc cgctggtcgc
     4801 ggatctactg gcgctgctgg aatcgcgctc cccgttgccg cgattcgagg atgacctgcg
     4861 cgcccgcatc gatgcgctgc atcgcttccg cgatcgcggg atgcgcgcgg ggggccacgc
     4921 ggattcggcg gcgctggcgg cgatcgagca gaccgcgcgc ggctggcgca agcgcctcgg
     4981 cctgcgccac ggcgccagcg gcacgccgga tgcactcgcg gtcggcaacc ttttgctgca
     5041 cgcatttccc gaccgcgtcg cccggcgcga cgagaaggat cctcgccgtt accgcctggc
     5101 caacgggcgt ggcgcacggc tgcgcgagga ttcttcgttg tacggcgagc cctggctggt
     5161 gattgccgaa gcgcgtttcg atgcacgcga cagcctggtg ctcgcagcgg caccgttcga
     5221 tcccgagcgc ctgcggctcg accatgcggc gcggatcgcg catgagcgca tcctccgcac
     5281 gaatgcgaac gacgtgctcg aagccttcga ggaagcccgc ttcggcgcca tcgtgctgga
     5341 gtgccgtgcg gtgccggtga atccggagga tacccttgcg gcgctgctgg cgacgatccg
     5401 gtcgcgcggc atcgctgcgt tgccgtggag cgacaccgcg ttgcggctgc gcgctcgcgt
     5461 cgggtggctg cggcaggcaa tgcccgacgc cggactgccg gatgtctcgg atgccgccct
     5521 gctcgattcg ctggaggcct ggctcgccac cgcgctccgc ggcaaacgga aactggaacg
     5581 gctgtcagcc gcggaattgt cgaacacgtt cgcgaacctg ctcgactggt cgcagcgcaa
     5641 gctcgtggac gaggcggcac ccgacgccct gcaggtgcca agtggcatga cccgcaaact
     5701 cgattacgcc gcggcttccc gaaccgcgga aggaatcccg tcaccggtcc tggcggtcaa
     5761 gctgcaggag ctgttcggcc tcgcggaaac cccgcgcgtc gcccgcggcc gggtgcccat
     5821 cacgttgcac ctcctgtcac ctgccggtcg cccgatccag gtgacccagg acctgcgcgg
     5881 cttctgggaa cgcacctatc ccgaggtgaa gaaggaattg aagggcaggt acccgaaaca
     5941 cccgtggccg gacgatccct ggtcggccat tccgacccac cgcgccaagc gacgttcgtg
     6001 aagcgcgacg atacccgtcg ggaggcttca atccttcagc gtgtactcga gcgtggccac
     6061 gacccgtacg gtcttttcga tctggcggtc ggcttcgagc atgggggcct tgtcgcgtgg
     6121 caggatctcg aacacgccct ggttggcgcg ccggatgccg ccgagctcgc tgccggaatc
     6181 gcgggcgaac tgcccggccg cggtacgtgc attcttcgtg gcttcggcga tcatcgacgg
     6241 cttcagcgcg ttgatgccgt cgaacaggta attcggaccg gtgccgccgc cgccatccgc
     6301 attgccgagc acgatgccct gggcaatcag cgccccggtg tcctgggcag ccgccgcgac
     6361 cgcgcgcacg tcccggctcc ggaccatgat cgtgccggtg acgacgaagc gtgtcgtgta
     6421 ctggtcgttg ccgtacatcc gcgccgcacg gtcggccacc tggacgtcgc ggaacgcgat
     6481 cgcgtcgtcg cccagcttgt gccgcgagag gaacgcggtg accgccgcgg cgtcacgctg
     6541 gatgctggcc tgcacgtccg cgagctggtt gccggtttcc acgaattcga cccgccatag
     6601 cgcgatgtcg gcctcgacgt cgtgttcgga cagtcccttc accgtgacga agcgctcctg
     6661 ccgcgattgc gcgaagcccg tgccgatgaa ccagccaccg atgacgaggc cggtggcaac
     6721 gatcagggcg gcgaagatgc gggtcatggc gggcagcggt cggttcggtc gggtgcagtc
     6781 tgccacggcg accgcggata cgcatcgcgg gttcgaggcc gaccggtggc gccgtcaggg
     6841 cttgcggtgc tggccgccat cctgctgcca ggaagccggt ccgaccgcgt gtccgccgta
     6901 cttttcggat gccccggggt aacgctggtc gacggggttg ccgtcggggc cctgcttcgg
     6961 cgaacgcgcg aagctgccgg agaagcccgg gaactcggcc gggctttccc agccatcctt
     7021 gctgaccgcc gacaccccga agaagtagtc gtcgaggatg atgtccttca acaccaccga
     7081 atcggcatcg cctgccttct gcaagtgttg ccattgcggc gcggtggtct cgcgccacca
     7141 cgcgttgtag ctcgcggcgc ccggcgacgg ctgccacgac agcgtggtgt cgtacgcgac
     7201 cgcgcccttc acggtgaggt cggccggtgg tggcggcgcc atcgcgagcg aggccatcgt
     7261 gatcgcatcg agccgtgtga cgttggcgag gtagtcgaag tccacgccgg atacgaggtc
     7321 gccgtagtgc acgccgttct cgacgcgcac gttctggtgc tggtggtcgt agttctcgtt
     7381 cgattccgaa agccgcaccg ccggaaaacc ctgttcgagg aacggcacct ggtcgcccga
     7441 gcggccccag cgatcagtgc gatacaccat gcgcacgcgg aagtcgtcga ggtactgctc
     7501 ggccagtgcc gccatgtagc gcgcgatgtt gcgcgagggc gaatccacgc cgccaccgtg
     7561 gtagcggcgg tacgcggcct gctccagcgt ctcgttgctc ttggtgcctt cggagaacac
     7621 ccggatcgtg tgcggatcgt gggcgccgtt gccgccgtgc gagttgccga ccatgtcgtt
     7681 gttgaggttg gcctcgacct tccagccatg atcgaccgcg tactgcgcga tgatcttgcc
     7741 gccgtacagg ccctgttcct cgccggaatc ggcggagtac accagggtcg cggggaactt
     7801 gtacttcgac agcacccgcg cggtttcgat cagtgcggcg acgccggccg catcgtcatt
     7861 ggcgccgggg gagtcgccgg tcgcgtccat cacgtcgctg cgccgcgagt cgaggtgcgc
     7921 ggtcatcacg atcacccgat ccggatcggt ggtgccgcgc tggatcgcga cgatgtccat
     7981 caccttcgca ccgtccttcg gcatgcgtgg gccggtgaac gactgcgcgg gcttcacgac
     8041 ctcgatgcag ccgttgcagc ctcgggaaat atcctggaac tgcgccgcga cccagcggcg
     8101 cgcggcgccg atgccgcggg tcttcgaggt cgtggtcgaa agcgtgtggc gcgtgccgaa
     8161 gccgaccagg gcgacatcgg tggcgtgcag gttggacgcc ttcaccgccg ccgcgatcga
     8221 ccgcagctcg ggatgatggt tcgccggcgg cgccgcagcc caggccgtag cggtcagcaa
     8281 cgcgcccgca gcgagcgcgg tcaacaagcg ggtacgcagc atggctgtcc ctccggatcg
     8341 atccggagag cctaacccgc gggcgggacg aatgcacgca ccgacacgca gcgcgcggca
     8401 tcgtcgggca tgcgccaggc ggcgatgcgc cggctgcccg acagcagata cggccaagcc
     8461 atgaaaggcg tgcccgccgc gggttcctcg tgccgctccc gggtcagcag gatgccgccg
     8521 ggatgcgctt tgcaccacga ctgcacgccg tcgagcggga ccgacggaac cggtcgatgc
     8581 aagcgtccgg cgtagccgaa caggccctcg ccgggctgca cgaacgcaat cgcactatcg
     8641 gccagcagcg cgttccgcac gaacgcggcc tccgggcgca cgtccagcgc cggcaggtac
     8701 gcaagcgccg cggccagcat tcccgctgac accagcgcgg tggcggacag cgccatcccg
     8761 cgcgcatcgc gtgcccaccc gcgccatgcg cctgccgcga cgaccaaccc gaccagcgcg
     8821 aacaaggcga tggcgggttg cggcggataa ccgaagagtt tcgccgcgtg ccacggcgcc
     8881 accgcgacca gtgcggcggc cagcagtgca agcaggccga acgacaatga ccgcaagcgg
     8941 cctcccggct gctgcagcaa ccacgcaccg gccacacccc acgccggcaa cagtggcagg
     9001 aggtagtgga cctgcttgcc gctgatcgca cagaaggcga cgaatggaac cacccaccag
     9061 ctccacagga accgtccgaa ccggccgcgc cacagcgccg cccaggctgt ccgcggcgca
     9121 cgcagggtca acacccacgg cagcagcaac accggtacca gcggcaggta ccaccaccac
     9181 ggccgccggt gggcgaagct gttcgcgatg cgcccggtga ccttgtccac cagcggctgc
     9241 caataggtcg gtccggcgta cgcagccgcc ggcagcagcc atgcgagcat gatcgcgatg
     9301 ccgatcgcga ggcccgccag cagcgccagg taccagcgca gcttgtgcgc ccgcgcggtg
     9361 ccgctccaaa gcggggccaa caacgcgacg gacacgatgt gcagcagcat cactggtccc
     9421 ttcgcgagga tgccgaggcc gaccgtgatg ccgagcgcca ccacgccgcg tgtccacttt
     9481 ccctcgtcga ggtcgagcag cgcgtgcaag gcgccgagca cgcaaagcga cagcagcagg
     9541 tcgaacatga tcgcggtggc gaacaccgtg aaaccgaggc agccggcgag tatccacatc
     9601 gcatcggcgg cgccgcgcgc cgtcatgccg aggcgcgcgg ccagccgccg cagcgccagc
     9661 agcatgcaca aggcgacgat cagcgcctcg acacgcgcaa cccaggtgtg cacgccgaaa
     9721 atcttccaac cgcccgcgat cagccagaac aggagcggcg acttgtccga gtaccacgcc
     9781 ccgttcacgg tcggaaccag ccactgcccg gaattgaaca tttcccaggc ggcggtgaga
     9841 tagcgggttt cgtcgatcgg gatcggcggg aacaggatgg cgagcggcag cagcagcacg
     9901 aaccacaatg ccagccagcg ccaaccgccg acgcttccga tgcgctgcgg attgatggca
     9961 gtcccgttca tcgcgcgcaa gcatagccga gccggaaaca agatcgccgg cgtcggccgg
    10021 cgatcttgtg ggttcggcgc acggccccgc tatttgcgct tcagatcgcg cggcttcggc
    10081 ggctcgcctt ccttgccgag ttcctcgagc aaggtcttca tgtcctcggc gtgctcttcc
    10141 tcctgcgcca ggacttcttc catgattcgg cgggtggtgg gatcgtcgtt gccgaggtac
    10201 tggatgatct cgcgataact gtcgatcgcg atccgctcgg ccacgaggtc ttccttgatc
    10261 atgtcgacga ggtcgtcgcc ctcgacgtaa tcggaatgcg cgcgcgaggt caggccttcg
    10321 ggattgaggt tgggcgcacc gtcgagctgg gtgatgcgct cggcaatctt gtccgcgtgc
    10381 tgctgctcct cgcccgcgtg ttccaggaat tccgccgcga cgctctgcgc gtggatgccc
    10441 gaagccatgt agtagtggta cttgtaacgc agcacgcaca ccagttccgt cgcgagcgcc
    10501 tcgttgagca ccttgatcac ggtgtcgcga tcggccgtgt agccatgggt gaccgcaccc
    10561 ttctcgatgt gctcgcgggc gcgctggcga atggtctcga tgtcggacag aaaaggtttc
    10621 gtggcggcca tgcgggtctc cttgggatca tgtcatcacg actgcgtcac gccagtgtac
    10681 gcccgcgatg ttgcggccag gtgaaagtca cgcggaccga tatacctctc cgccgtgctc
    10741 gcggaactcg gcggcctttt cggccatgcc ttcgtcgagc gcctcggtct catcgacgcc
    10801 gtgttcgcgc gcgtagtcgc gcacgtcctg ggtgatcttc atcgagcaga acttcgggcc
    10861 gcacatcgaa cagaaatgcg cggtcttggc ggcgtccttg ggcagggttt cgtcgtggaa
    10921 ttccttggcc ttctcgggat cgaggccgag gttgaactgg tcttcccagc gaaactcgaa
    10981 gcgcgccttc gaaagcgcgt tgtcgcgcgc ctgtgcgcca ggatggccct tgccgagatc
    11041 ggccgcgtgc gctgcgatct tgtaggcgac gatgccttcg cgcacgtcct ccttgtctgg
    11101 aagccccaag tgctccttgg gcgtcacgta gcaaagcatc gcggtgccgt accagccgat
    11161 catcgcggcg ccgattgccg aggtgatgtg gtcgtagccg ggcgcgatgt cggtggtgag
    11221 cggccccagc gtatagaacg gtgcttcgtg gcacttgtcg agctgccgct ccatgttctc
    11281 cttgatgagg tgcatcggca catggcccgg accttcgatc atcacctgca cgtcgtgctt
    11341 ccaggcgatg tgggtcagct cgcccagcgt gtcgagttcg gcgaactgcg cctcgtcgtt
    11401 ggcgtcggcg atgcagccgg gccgcaggcc gtcgcccagc gagaaggcga cgtcgtacgc
    11461 cttcatgatt tcgcacattt cctcgaaatg cgtgtagagg aaggattccc tgtggtgcgc
    11521 gagacaccac ttggccatga tcgatccgcc gcgcgagacg atgccggtga cgcgcttggc
    11581 ggtcagcggc acgaagcgca gcagcacgcc cgcatggatc gtgaagtaat cgacgccctg
    11641 ttcggcctgc tcgatcagcg tgtcgcggaa cagctcccac gtcagttcct cggctgcgcc
    11701 gccgaccttt tccagcgcct ggtaaatcgg cacggtgccg atcggcaccg gcgaattgcg
    11761 catgatccac tcgcgggttt cgtggatgta cttgccggtc gaaagatcca tcacggtgtc
    11821 gccgccccag cggatcgccc acaccatctt ttcgacttcc tcggcaatgg aagacgtgac
    11881 cgccgaggtg ccgatgttgg cgttgatctt ggtgaggaag ttgcggccga tgatcatcgg
    11941 ctcggtttcg ggatggttga cgttgcaggg gatgatcgcg cggccgcgtg ccacttcgtc
    12001 gcgcacgaat tccggcgtga tctcgcgcgg gatcgcggcg ccgaaggatt ggcccgggtg
    12061 ctgctgcgcc agcgcgctgc cgcgcaacgc atcgagtttc tggttctcgc ggatcgcgat
    12121 gaattccatt tccggggtga cgatgccttg ccgcgcgtag tgcatctggg tgacgttggc
    12181 gccggccttg gcgcggcgcg gtttcggcag gttcgggaaa cgcacgtcgg cgagcagcgc
    12241 cgcttccgca tggcggcggc cgaatttgga ggtgcgattc ggcagcacct cggtgtcggc
    12301 gcgctcttcg atccatgctg cgcggagcgg cggcagcccg gcaacgaggt cggccttgaa
    12361 ggcgggatcc gtgtatggcc cggacgtgtc gtagacggtg aacggtgcgt tggcttccac
    12421 tccgaacatc gccggcgttt ccgtcagcac gacttcgcgc atcggcacgc ggatgtcgtc
    12481 gcggctgccc gtggcgtacg tcttgcggga accccggatc ggcgaggtga cggttgcgga
    12541 aagctgctgc gcttcccgca ggaggtcgga aggctgggca ttcatcggcg tcgctccgga
    12601 ttcagggagg gagcgcgaat cctgcatcgc tgcacgcgcg cctacgcgtg cttcttcgca
    12661 gagcgatccg cgcttgaata cgggcggcgc cggtcgcagg ggcacgccca tgcggcgaag
    12721 ctccctacac cggtgcgaac cggatcaggt tccaagggtt tctctcagcc gatgcggtcc
    12781 atcgcgagcg gcacccccgc ttcaagccac cattcaagca cgtttctcgt gcgatcgtcc
    12841 atgatcctgt ttctcatgcg atcgtccatg atcgctgcct cgatgggtgc aggcgcggcc
    12901 cgacgatccg aagtccggaa acaccacaac ggccatccat ggcctgactt ttcgcaacag
    12961 ccgctccgca tgctgcattc cgagcggcgc ttgttgaaga tagtcgtgaa gtcgaaaagc
    13021 ttcggacatg gatcggcgcg gatcaggcgg gtctttcttg atgatccgcg tgcatccgcg
    13081 taaacccgcg tcaaaaagat gcttggatcc gcggtagtgc cgcgaccggg agcgggcccg
    13141 cgcgggttac cgccgcccgt acgcccggag gaacatgtcc accgtggctt ccagatgcgc
    13201 ctcggcctct tcatggtccg gcttgccgca gccgaagcac atgcgcgcgt ggtgctcgcc
    13261 cttgagcagg gcgaagaact ggcaggcggc ccgccgggtg tcggcgatgt cgagttggcg
    13321 tgcgtccacc tgcttttgca gcatcgacgc gagcccggcc tgtacctgca tcgggccggc
    13381 ctcccagaac agcttggcga gcttgggcga ttgctgcgag ctcgcgacca gggtgcggtg
    13441 catgcccagc gcaccgtcgc tcatcatcag gtcgaagaag gcgcgcgcaa tgccgagcaa
    13501 ctggctgcgc accggtgcat ggacgtccac ctcgaaaatc aggcccggca gcatgccttc
    13561 gcacttgcag cgcacggtct cgacgaacaa ggcttccttg ccgccgaaat ggctgtacac
    13621 cgtcagcttc gacacgcccg cctcggatgc caccgcgtcc atgctggtgc cctcgaaccc
    13681 gtgctgcggg aacaggcgct tggccgcctc caggatcgcg gcgcgctttt cgaggtcctt
    13741 gggccggccg ggtccggcgc agcgttcggg agaggcgttc atcaggggtg tcgattcatc
    13801 aaagcaggtc caggcgttat tatacgcgcg agtacatatt tcttcccgcg gccttccacc
    13861 gggccctgtc cgaggcgtcc gtggcgctgt ccgcggacac gccacgggcg ccggcgcgtg
    13921 cgccatcgcc ttgatatgcg ggggaaaacg cgccgtggat tgctggcccg gaacgtgcaa
    13981 tgggaacaac gcgggcgggg tgaccgctgt caccgcggtc tggtgacttc cggcacttcg
    14041 tcgtctcggg ggccgccggc aacctgatcg cgttgcccgc gccacgacgc ccgcctaagc
    14101 ttgtacggtt ttgggcagca ggactctgca gatatgaact tgaatccgcc ctccgaagtc
    14161 gcccgacgca acatggtcga aaaccaggtc cgcgcatggg aagtactcga tgcgcgcgtg
    14221 ctggacgtgc tggtcgcgct gccgcgcgag gcgttcgtcc ccaagcccta cgaggcgctc
    14281 gcgtatgccg acgtcacgct gcccatcggc tacgacgagg tgatgctgaa gccggtcgtc
    14341 gagggccgct cgctgcaggc gctgctgccg ctggccagcg agcgcgtgct cgagatcggc
    14401 accggcaccg gctatttcgc ggcctgcctt tcacggctcg ccgcgcacgt gaccagcatc
    14461 gacatccggg ccgacttcgt ggaggccgcg cgccagcgcc tgctggacct cgccatcgac
    14521 aacgtcgagt gcgtgcacgc cgatgccttt tccggattcg cgcccgacgc gcccttcgat
    14581 gcgatcgccg tgaccggcgc ggtcgccgag ttgccgccga tcttcggcga atggctggag
    14641 ccggacggcc gcatgttcgt gttccagggc tttgcgccgg cgatgcacgc ggtgctgctg
    14701 gaacgcgccg ccgacggcgg ctggatcgag gaccgccgat tcgaaaccga cgtcccgtac
    14761 ctgaccgggg cagggcccag gccgcagttc cacctgtagg agcggcccat ggccgcgatg
    14821 gagcgttccg tcgcgcgcat gacccgttcc cacgaaaacg attgaaaccg gattacacgc
    14881 gccgacgcgc caaggattct cccgcatgac ccaaagcccc gtgtcccgcc accgcttcgt
    14941 gccccgcgcc ctcgcgattg ccctcgtggc ggcgctcggc ctcgccggga ccgcgacagc
    15001 gcacgccgag aacctcatgg acgcgtatcg ccaggcgctg cagtcggacc ccgtgctgca
    15061 gcaggccgag gcgcagagcc gaatcggcga agcgggcgcg gacatttcgc gctcggcact
    15121 gctgccgcag ctcaatggcc aggtctcgtt caacgacagc cacggcatct cgagcgggac
    15181 ccaggttttc tacgaccaga acggcaatcc cacgctcgag gacaccacgg tgcatacggg
    15241 cggccgctcc cggaccgagt ccgtcgggct caaccaggtg ctgttcgacc tcgggcagtt
    15301 ctccacatgg cgcgccagca aggccagcgc gagggccgcc gaggcgcaat acacggccgc
    15361 gcagcaggac ctgatcctca gggtggcgca ggcgtacttc acggtgctca gcgacgagga
    15421 gcagctcaaa tacgccgagg ccaacgagaa ggcgctccag aagcagctcg accaggccca
    15481 ggccaagtac gacgtgggct tcgcggcgat caccgacgtg gtggacgcca aggcacagca
    15541 cgacaccgcg gtcgccaacg tcatcaacgt caagaaccag gtcttcaacg accgccaggc
    15601 actcgcgcag atcacggggc agccgcccgg cacgctcgag acgttgaccg acaagttgcc
    15661 gttggcgccg ccgcagcccg acgacatgca ggagtgggtc aacacggcgc tggccagcaa
    15721 tccgtcgctg caggcgcaac gcgagcagaa ggaagccgcg cagcacgacg tcacggcggc
    15781 ctacgccggc caccttccga ccctggatgc gtcggtcagc tattcgcgca acccgtcgtg
    15841 gggtggcgtc ggtttcacga gctccggcgc gccgcgcggt gacagtcgca gcagcggcac
    15901 caccgtggga ctggtgctga ccgtgccgct gttctccggt ggcgggatcc atgcgagggt
    15961 caagcaggcc atcgcgcagc gcgaccaggc tggcgacgtg ctggaacagg atcggcgcac
    16021 ggtggtcagc aacacccgca gcgccttcaa cgcgatcgag gccgggatct cttcggtcga
    16081 agcgcaaaag gccgcggtgg aatccgccaa gaccgccctg gacgcgagcg aggccggcta
    16141 ccagatcggt acccgcacca tcgtcgacct gctgctcgcc cagcagacct acttccaggc
    16201 gcaaagcgcc tattcgcaag cgcgccatgc gctggtggtc aaccggctga acctcgatta
    16261 tgccgccggc acgctgtcgg tggacgacct cgaagccgtg aaccggctgt tgcagtaacg
    16321 gaaggcttgc ccgcgcggcc gtcgtcggcc ggtcgccgcg tgcggagcag cctcagtccg
    16381 tccgcgcgct cgaggcgtag cgtgcgcgcg cgacgatccg gccgagccac accatggtcc
    16441 tgcgcgcggc gccccgctcg cgggcgcaca ccgcgcgtgc cgccgccccc atgcgcgcga
    16501 gctcgtcgcc attgcgcagc aggtccagga ccatcggacc cagttccgcc gccgccgcga
    16561 cccggcgcgc gccgccggcc tcgatcaggg tgcgggtgat ttcctcgaag ttgaaggtgt
    16621 tgggtccgac caccaccggc ttcgacaggg cagcgggttc cagcacgttg tgtccaccga
    16681 tcgagaccag cgagccgccg acgaaggcga ggtcggccgc ggcgaagtag cgcatcatca
    16741 cgcccatcgc gtcgatcacg aggcactgcg tctcctcgtc cacgtcgtcc acgctatgcg
    16801 tggccacctt gaagcccagg ttgcgggcgg cgtgttcgac catccggaag cgctccggat
    16861 ggcgcggtgc gaccagcagc agcgcgtcgg gcatccgcgc gagcacggtg aggtgcgcct
    16921 tgaacgccgc cagttcctcg ccttcgtggg tgctggccgc gatccacacg ggacgcatgt
    16981 cgccccattt gtcccgcagg tcctcgccat gctgccgcgc gcccttgggg agcggcatgt
    17041 cgtacttcag gttgccggat acgtgcacgc gcgagggttg cgcacccagg gtgcggtagc
    17101 ggaccgcatc cgcgcgcgac tgcgcggcga ccagcgcgac gttgccgagc gcctgccgca
    17161 ccagggcgcg gatcggccgg tagcggcgca gcgatcgttc cgacagccgc gcattcacca
    17221 ccgccagcgg aatcccgcgg cggccacact ggtggaacag gttcggccag atctcggttt
    17281 ccatcaccac cgcgagcgcg ggacggatgc ggtcgaggaa cctggagacc gcggccggca
    17341 ggtcgtaggg cagatagacg tggaacacct cgtcgccgaa aaggttgtgg acccgttcgg
    17401 agccggtggg ggtcacggtg gtgacgacca tcggccgcgg cgcgtagcgc ttgcgcagcg
    17461 agtggatcag cgggatcgcc gcgttgacct cgccgaccga caccgcgtgg acccagatgc
    17521 agtcgtgcag ccccggatcg ggaaagcggc cgaaacgctc gcgccagcgc ccgagatagc
    17581 tccggtagcg gacgccgtgc accaccaggc gggccacgat gaccggcgtg acgagataca
    17641 tcgcgagcgt atacaggaat cgcagcatgg cgcggttcga agccggcagc gggcgagtat
    17701 aactttggtg cgcgccg
//
LOCUS       DASSNH010000116         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1527608, whole genome shotgun sequence.
ACCESSION   DASSNH010000116 DASSNH010000000
VERSION     DASSNH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527608"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /gene="gloB"
                     /locus_tag="VFK00_04175"
     CDS             complement(<1..678)
                     /gene="gloB"
                     /locus_tag="VFK00_04175"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET7592186.1"
                     /translation="MSSLRALPALRDNYIWMLSDPASGAALVVDPGEADPVLRALAED
                     GRTLAAILLTHHHADHIGGTAELKSLFPDATIYAPIDDRIHLAATRVGDGDVIQLSAP
                     LARFEVIEVHGHTRSHIAYQGEGLVFCGDTLFSLGCGRMFEGTPEEMLASLDRLAGLP
                     ADTQVCCGHEYTLANASFAISVEPDNPALQARMRDARAQRERGEPTVPARLADERAAN
                     PFLRVDAI"
     gene            828..1547
                     /locus_tag="VFK00_04180"
     CDS             828..1547
                     /locus_tag="VFK00_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7592187.1"
                     /translation="MPTPRSDVHDPALAAIGADAAAVCARRLTRIAGIHALVLDGIAL
                     PEPRVEGVACWTTLERDTAGNGWRGPLRMDVHELPFGDESFCAVLVRFAEHAAIAPED
                     LSRELARVLANHGTLLVSGLHPRSWWRDGLPPRRWERALRAAGLDVLPAIRCGAPWPR
                     AAGADGMPTWLVRGMGGAYVIEAHRRIVTAIPLRKSAAGRRAVDQKAFLPNARRKSLH
                     GLLRSAGSALRAARRVSRTRA"
     gene            1544..2002
                     /gene="rnhA"
                     /locus_tag="VFK00_04185"
     CDS             1544..2002
                     /gene="rnhA"
                     /locus_tag="VFK00_04185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HET7592188.1"
                     /translation="MTANGSHVVKVFTDGACLGNPGPGGWAALLRWRGTEKLLAGGEA
                     ATTNNRMELMAAIQALEALKRECEVLLTTDSQYVRQGVEQWLARWQANGWRTAGKQAV
                     KNQDLWERLAAASARHRVRWAWVRGHSGHPENERVDRAARAEAEQLRELA"
     gene            1999..2736
                     /gene="dnaQ"
                     /locus_tag="VFK00_04190"
     CDS             1999..2736
                     /gene="dnaQ"
                     /locus_tag="VFK00_04190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HET7592189.1"
                     /translation="MTRKVILDTETTGLEVERGHRVIEIGCVELVDRRPSGRTFQRYL
                     NPERLIEAGAVAVHGITDEFLRDKPKFADIVEDFLAFVDGAELLIHNAAFDTAFLDDE
                     LKRAGAGYGRLADRVTIVDTLELARRKYPGQKNTLDALCKRLGVDNSGRKVHGALLDA
                     HLLADAWIAMTAGQAALALESGPSGPAAGAVVAGVAIGELLARPRVLRASALEAAAHA
                     ARLDALGKASGDASLWRRAESVDEACA"
     gene            complement(2881..3591)
                     /locus_tag="VFK00_04195"
     CDS             complement(2881..3591)
                     /locus_tag="VFK00_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET7592190.1"
                     /translation="MIEFGHATHAGLRREHNEDTYWADADLGLFLVADGLGGHEHGEL
                     ASALARDAVVAATRGGKGLSDAALRANDEILAYPHPDGATVAPMGTTLAALRLGGDYG
                     FEIAWVGDSRIYLWSQGALKQLSRDDSVVQELVEQGTITADEARSHPQRNQITQALGI
                     TPPETLKIQPLAGRCSAGMQFLLCTDGLTEELDDTRIAAVLSRTDLAAQECVDHLLLE
                     ALDAGGRDNITVVLVRIR"
     gene            complement(3635..>3917)
                     /locus_tag="VFK00_04200"
     CDS             complement(3635..>3917)
                     /locus_tag="VFK00_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592191.1"
                     /translation="CDNVARSPNPSLYGTHTEDGVEVGGLEVGLDRARSDNVQVRMRG
                     LALWLALVYHETRNAAPTPENEELHRQVLRIMRELKVFSSRLESGGSGA"
BASE COUNT          582 a         1310 c         1404 g          621 t
ORIGIN      
        1 gatggcgtcc acgcgcagga atggattcgc ggcgcgctcg tcggcgagcc tcgcgggcac
       61 ggtgggctcg ccgcgttctc gttgggcgcg cgcgtcccgc atgcgcgcct gcaaggcagg
      121 gttgtccggt tccacgctga tcgcgaagct ggcattcgcc agcgtgtatt cgtgcccgca
      181 acacacctgc gtgtcggccg gcaggccggc gagccggtcc agcgacgcga gcatctcctc
      241 gggcgtgcct tcgaacatcc ggccgcagcc gaggctgaac aaggtgtctc cgcagaaaac
      301 caggccttcg ccctggtagg cgatgtgact gcgggtatgg ccgtgcacct cgatgacttc
      361 gaagcgcgcc agtggtgcgg aaagctggat cacgtcgccg tcgccgacac gcgtggcggc
      421 gagatggatt cgatcgtcga tcggcgcgta gatcgtcgcg tcgggaaaca gcgacttcag
      481 ttccgctgtc ccgccgatgt ggtcggcgtg gtggtgggtg agcaggatcg ccgcgagtgt
      541 gcgaccgtct tccgcgagcg cgcgcaggac cggatcggct tcgccggggt cgacgaccag
      601 cgccgcgccg gaggccggat cggaaagcat ccagatgtaa ttgtcgcgca gggcgggcag
      661 ggcacgcagg gacgacatgg caggttcggt gaaaacccaa ccttaacagt cgtgccggcg
      721 acgttcgtta ggtcaggctt aacgggaggc tgcggcttat cgtttgctca tgaatccgtc
      781 acaatgcgga ctagatcccg cttccgatgc tgcgattgcc ccccacgatg ccgacaccca
      841 gatccgacgt gcacgatccg gccttggcgg ccatcggtgc cgacgcggcg gcggtctgtg
      901 cgcgccggct gacgcggatc gccggcatcc acgccttggt gctggacggc attgcgctgc
      961 cggagccgcg ggtggaaggc gtggcatgct ggaccaccct cgaacgcgac accgccggca
     1021 acgggtggcg cggaccgctc cggatggacg tgcacgagct tcccttcggc gatgaatcct
     1081 tctgtgcggt gctggtgcgc ttcgccgaac acgcggcgat cgcgccggag gacctctcgc
     1141 gcgaactcgc gcgggtcctc gccaaccacg gcaccttgct cgtttccggt ttgcacccgc
     1201 gttcctggtg gcgggacggc ttgccgccgc gtcgctggga gcgcgcactg cgcgccgccg
     1261 ggctcgacgt gctcccggcc atacgttgcg gtgctccctg gccgcgcgcg gcgggcgcgg
     1321 acggcatgcc gacgtggctg gtgcggggca tgggcggcgc ctacgtgatc gaggcgcatc
     1381 ggcggatcgt cacggcgatt ccgctgcgca agtccgcggc cggccggcgc gccgtggacc
     1441 agaaggcgtt cctgccgaac gcccgccgca agtcgctgca cggcctgctg cgctccgcgg
     1501 gttcggcgtt gcgggccgcc cggcgagtgt cccggacgcg tgcatgacgg cgaacggttc
     1561 gcatgtcgtc aaagtgttca ccgacggcgc ctgcctcggc aatccgggtc ccggcggctg
     1621 ggccgcgctg ctgcgctggc gcggcaccga gaagctgctc gccggcgggg aagccgccac
     1681 caccaacaac cggatggagc tgatggccgc gatccaggcg ctcgaggcgt tgaagcgcga
     1741 atgcgaggtg ctccttacca ccgactcgca gtacgtccgg cagggcgtcg agcagtggct
     1801 ggcccgctgg caagccaatg gctggcgaac cgccggcaag caagcggtca agaaccagga
     1861 cctgtgggag cgcctggccg ctgcctcggc ccgccaccgg gtacgctggg cgtgggtgcg
     1921 cgggcacagc ggacatccgg aaaacgagcg ggtcgatcgc gccgcccgcg ccgaagcgga
     1981 acaactcagg gagctcgcgt gacgcgcaaa gtcattctgg ataccgagac gaccggcctc
     2041 gaggtcgaac gcggccatcg cgtgatcgag atcggttgcg tcgagcttgt cgatcgtcgc
     2101 ccgagcgggc ggacgttcca gcgctacctg aatcccgagc gcctgatcga agcgggcgcg
     2161 gttgcggtgc acggcatcac cgacgagttc ctgcgcgaca agccgaagtt cgcggacatc
     2221 gtcgaggact tcctggcatt cgtcgatggc gccgagctcc tgatccacaa tgccgcattc
     2281 gacaccgcgt tcctggacga cgagctgaag cgcgccggcg cggggtacgg caggctcgcc
     2341 gaccgggtga cgatcgtcga caccttggag ctcgcgcgcc ggaagtatcc gggccagaag
     2401 aacacgctcg atgcgctgtg caagcgcctc ggcgtcgaca atagcggccg caaggtccac
     2461 ggcgccttgc tcgacgcgca cctgctggcc gacgcgtgga tcgcgatgac cgcgggccag
     2521 gcggcactgg cgctcgaaag cggtccctcg ggccctgcgg ctggcgcggt ggtggcgggc
     2581 gtggcgatcg gcgaactgct ggcacgtccg cgcgtgttgc gtgcctcggc gctcgaagcg
     2641 gcggcacatg ccgcgcggct ggatgcgctc ggcaaggcca gtggcgacgc cagcctgtgg
     2701 cggcgcgccg aaagtgtcga tgaggcttgc gcgtagggcg gaatcggcag ccgcttccgc
     2761 caagcatcga atgtcgaatc gggagaaacc ctgcgcgggt tacgccctgt tttcaaagcg
     2821 ggtgcaggcg gacgcgcggc cgggaagcaa gtgccttgaa gcgtcacggc cgccgtgcgg
     2881 tcagcggatc cgcaccagca cgaccgtgat gttgtcccgc cccccggcgt cgagcgcctc
     2941 cagcagcagg tgatcgacgc attcctgcgc ggcgagatcg gtgcgcgaca gcaccgcggc
     3001 gatgcgcgtg tcgtccaact cctcggtcag gccatcggtg cacagcagga actgcatgcc
     3061 tgccgaacag cgccccgcca acggctggat cttcaaggtt tcgggcggag tgatgccgag
     3121 tgcctgggtg atctggttgc gctgcggatg gctgcgcgcc tcgtcggcgg tgatggtgcc
     3181 ctgctcgacg agttcctgga ccacggagtc gtcgcgcgaa agctgtttca gcgcgccctg
     3241 cgaccacagg tagatgcggc tgtcgccgac ccaggcgatt tcgaaaccgt agtcgccgcc
     3301 gagccggagc gccgccagcg tcgtgcccat cggcgcgacc gtggcgccgt ccgggtgcgg
     3361 gtaggccaga atctcgtcat tggcgcgcag cgccgcgtca ctcagaccct tgccgccgcg
     3421 cgttgcggcc acgaccgcgt cccgcgccaa ggcgctcgcg agttcgccgt gctcgtgccc
     3481 gcccagtccg tcggcgacca ggaacagccc gaggtccgca tcggcccagt aggtgtcttc
     3541 gttgtgctcc cggcgcaagc cggcgtgcgt cgcgtgtccg aattcgatca tggcacggtg
     3601 gcgtcggtcg cacggcccgg cgatggttgc ggtatcaagc tccgcttccg cccgattcga
     3661 gtcgcgagga aaacaccttc agctcgcgca tgatgcgcaa cacctgccga tgaagttcct
     3721 cgttttccgg cgtcggcgcg gcgttgcggg tttcgtggta caccagcgca agccacagcg
     3781 ccaggccgcg catgcgcacc tgcacgttgt cggagcgggc gcggtcgagt cccacctcga
     3841 ggccgccgac ctcgacgccg tcttcggtat gcgtcccgta cagcgaaggg ttggggcttc
     3901 gcgcgacgtt gtcgcat
//
LOCUS       DASSNH010000117         6743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1135194, whole genome shotgun sequence.
ACCESSION   DASSNH010000117 DASSNH010000000
VERSION     DASSNH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135194"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(848..1390)
                     /locus_tag="VFK00_04205"
     CDS             complement(848..1390)
                     /locus_tag="VFK00_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7592192.1"
                     /translation="MTRALSHTFVATLAIGMTAAALAGSIEPGDHLEMAAVLKAQVTP
                     TQAVKIAEAGGGRAYGYGMEATRRGHWYEVEVLRGDAKLELRIDATSGKVIGSRPAHG
                     EDARGAHALDGNKLTFGEAIAQAERVGNGPALEANAAGHGNKAHVEVDVIQDHGKRIA
                     HYHVSMLGGQVKTTLTGTDA"
     gene            1528..2193
                     /locus_tag="VFK00_04210"
     CDS             1528..2193
                     /locus_tag="VFK00_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7592193.1"
                     /translation="MKLLLVEDSERLRDNLARGLRAAGFTVDVAVDGVEANGYLAGYA
                     YDFMVLDLGLPRLDGFGVLRALPVGGERPRVLVLSARDQVGDRVEALNAGADDYLVKP
                     FAFEEVVARLNALARRPAETHADVLEHAGVQLDAQAHRATANGAVLQLTPREFALLEL
                     LLRHRGRVFPRADILERIAGSESEASDRSIEVVVFGVRRKLADAGCASLIENRRGAGY
                     LIP"
     gene            2229..3575
                     /locus_tag="VFK00_04215"
     CDS             2229..3575
                     /locus_tag="VFK00_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HET7592194.1"
                     /translation="MTLLLALAVLVAFGAAAKIVDWRADTEMQQRFDASLLSRAQSLA
                     ALTQIENGRVAVDADNAGVNVFPGNADTSWYELRCGDSTVARTSEVPPSMSAGAEPVF
                     ADAQLPGGRLLRVVALRFVPATEGHRTTAANAPSCQLRYALDRGPLEDLLNTLDVILL
                     GSLLGACVVVMLATPWLVRRGLRPIAVLDDAMTGIGPDTPGARLLASTSTELAPLVVR
                     INEVLARMDEGLARERQFASGLAHEFRTRLAELRTLVDVETRYPSGRDARSLLCEAGS
                     IGEELEATVTALLQLTRIQSGLEQSQAESVPLRPLLARVRARHQDAARARGVGIEPGD
                     ACKPDFTIKADPVLLEIVLDNLLGNAVAYVPAGGIVSLGASENGIEVCNAAPALHPED
                     LVNFGRRFWRKDAQGAGHAGLGLALAAAAAKALRMTLSFELNEGVLRANVRWCTKD"
     gene            complement(3590..6652)
                     /locus_tag="VFK00_04220"
     CDS             complement(3590..6652)
                     /locus_tag="VFK00_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7592195.1"
                     /translation="MNIATWFQVHRRSLLFLVVILALGGLVSLFNLPVALFPDVSFPR
                     IRVSIDAGDRPADQMAVAVTRPVAEAIRQVRGVRNVRSTTSRGSAEINVDFDWGADMA
                     RAFLEVNAAASQVLPDLPSGTRIQAIRMDPTADEPVIAYSLRSKTLTPTQLYDIAQYQ
                     LRPLISGVPGVATVDVQGRGTAELHVNVDPAKLRAQGLGMADVVRAVGSAADISALGR
                     LADHYKLFLVLANNQPASVQALDDVVVRADPRGVVRVGDIATVTRGVKPEWVMTGANG
                     KPAILMQVFQQPAANSIAMVDAVKAKLASYTPQMPKGVEIAAWYDQTQLVRESAGSVR
                     DAILIGIVLAGLVLFVFLRNTRVILIAMLVVPATLAITVLLLKILHMSFNMMTLGGMA
                     AAVGLLIDDVVVMTEHIMRRVVSESGHVRERIMSAAHEFTRPQTGSSLATIVIFLPLA
                     FLTGVTGAFFKALSLTMASALVISWLLTLIVVPLLAQWLIGATHARERPPSHLWKRFM
                     GFYESVLLRVLEWPALLLLGLIPLLVLGFFAYKSVGSGFMPPMDEGGFTLDYRSLPGT
                     SLAETDRLLQKVDGILAADPYVDTWSRRTGLQLGGGLTEANSGDLFVRLKNVARPPTE
                     QVMEHIREQVAAKVPGLDVDVSQLLEDMIGDLTAVPQPIAVKLFSDNVTELDATANKV
                     AAQLGKIPGVVSVRNGINPAGDGLEVKVDPVQAALLGLDPRSVAGQVATAMAGMVAAE
                     LPEGPKMVGVRVWIPPRDRTTIEQLATLPIDDGHGHVFPLSRVASVTIQSGQPEIDHE
                     NLKRMLTVTGRISGRDLGSTMRDVTRVLSRRGELPPGMYYELGGLYQQQQIAFHGLTI
                     VMLTAIALVFTLLLFLYESFRAAVVVLVQPILAICGVFIGLWVTGTELDISSMMGMTM
                     IVGIVTELSIFFFSELADIQARADEDNPPPLRKALLQAGRGRTRAILMSTIAAILTLA
                     PLALAIGRGSQMQQPLAIAIIAGMLTAVPLVLVVMPVLYSLAMRRHGT"
BASE COUNT         1141 a         2173 c         2249 g         1180 t
ORIGIN      
        1 tcaagctcct cgttgcccgt cgagtctcgc cggcgcacag gtgcatcgcc ggctgcgcgt
       61 cttcgcaagc gtcggacgcg gtgcacgaag ccgatcgtga agccgtgtgg aagccagccc
      121 gggaccagcg gcaatggccg gtgcggccca ggtgccgacg ccccctaccg ggtgtcgagg
      181 cggttagtcc gtcgctctgc ccgtgtccac gcgtcctgac gtgcgtcacg gcgacgcagg
      241 gcggcggtgg ttccagcgaa tatacttcca gctgatcgcg atcgtggcgg cgacgaacgg
      301 tgtcaccgct ccggcaatgg cggtgacttc accgctggaa aagctctggt gtcgcgtgcc
      361 cttgaagaat tcgtccggat tggacgtctg cgagggacgc tcgcgctgga agatccattt
      421 gccgccttgt gcgaaccccg gccgacactg ccattgaatc cagcgagcgc cagagcgtgt
      481 cgcccagctt gtcttggtcg ccgaacgcgc gcgagccgcc cacgaccgtc agtgtcgctg
      541 tgcccatcag caccttccgg ttgctgcgcg ccagataggg ctgttgtcat aggcgatgcg
      601 atggttgatg ccgagcggac caccggcccg cgccacgcgc agcggtgccg ctgcgcagac
      661 gccgatcaac aggagcgttg caagcctccg catgaggatt ccggggtgcg tgccattcgt
      721 cgcccgcgtg cgataccggg tactttgtag ccagacgctt ggcgaacggt tcagcgcagc
      781 tttcaccaac cttgcaaggt tgccgcgagc ggcggcgccg cccgcggcag ccgccggctt
      841 ccgacgctca ggcatccgtg ccggtcagcg tggtcttgac ttggcctccg agcatcgaca
      901 cgtggtaatg cgcgatgcgc ttgccatggt cttggatgac gtcgacttcc acgtgggcct
      961 tgttgccgtg gcccgccgca ttagcttcca gcgcgggacc attgccaacc cgttcagcct
     1021 gcgcgatggc ttcaccgaat gtcagcttgt tgccgtcgag cgcgtgtgcg ccgcgggcat
     1081 cctcgccgtg ggccgggcgg ctgccgatca ccttgccgga cgtggcgtcg atacgcagtt
     1141 cgagcttggc atcaccgcgc aacacttcca cttcatacca atgtccgcgg cgcgtggctt
     1201 ccatgccgta gccataagcg cggccgccgc cggcctccgc gatcttcacg gcttgggtcg
     1261 gcgtaacctg cgccttgagc acggctgcca tttctaggtg atcgccgggt tcgatgctgc
     1321 ccgccaaggc tgcagcggtc atgccgatgg cgagggtggc gacgaaagtg tgggacaatg
     1381 cgcgtgtcat ggagaatctc cgtggggtgg agaattcagt ctgtggcacg agcctttgcg
     1441 cgcacttcgc gcgggcttag cgctcgctta gtagtggccg tgcgcgcgag gtgttagctt
     1501 tcccgcatta catgacgggc gccgcacatg aaactcctgc tggtcgagga ctccgaacgt
     1561 ttgcgtgaca acctcgcacg cggcctgcgc gcggccggat tcacggtgga tgtcgccgtg
     1621 gatggcgtgg aagccaacgg gtatctggcc ggttacgcgt acgatttcat ggtgctggat
     1681 cttggactcc cgcggctgga cggtttcggc gtgttgcgcg cgctgccggt gggcggtgaa
     1741 cgtccgcggg tgctcgtgct gtcggcgcgc gatcaggtcg gtgatcgcgt ggaagcattg
     1801 aatgccggcg ccgacgacta tctcgtcaag ccgtttgcgt tcgaggaagt ggtcgcgcgg
     1861 ctgaacgcgc tggcgcggcg tcccgcggaa acacatgcgg acgtgctgga gcacgcaggc
     1921 gtgcagttgg acgcgcaggc gcaccgcgcc accgcgaatg gcgccgtgtt gcagctcacg
     1981 ccacgcgaat tcgcgttgct tgaattgttg ctgcggcatc gcggacgggt tttcccgcgc
     2041 gccgacatcc tggaacgcat tgccggcagc gaaagcgagg cttccgatcg cagcatcgag
     2101 gtggtcgtgt tcggcgtgcg ccgcaagctg gccgacgcgg gttgcgcgag cctgatcgaa
     2161 aaccggcgcg gtgccgggta cctcattccg tgagtccgca cgcgaaaaca ttttcgcttc
     2221 gcacccgctt gacgctgctg ctggcgctgg ccgtgctggt ggcgtttggt gcggcggcca
     2281 aaatcgtgga ctggcgcgcc gacaccgaga tgcagcagcg tttcgatgcc tcgctgttgt
     2341 cacgcgcgca gtccctggcg gcgctgaccc agatcgagaa cggccgcgtt gccgtcgacg
     2401 cggacaatgc cggggtcaat gtttttcccg gcaacgcgga taccagctgg tacgaattgc
     2461 gctgcggcga cagcaccgtt gcgcgcactt cggaagtgcc cccgtcgatg agcgccgggg
     2521 cggagccggt attcgccgac gcgcaacttc cgggagggcg cctcctgcgc gtggtcgcct
     2581 tgcgatttgt gccggcaacg gaaggtcatc ggacgacggc cgccaatgca ccctcatgcc
     2641 aattgcgtta cgcgctcgac cgcggaccac tggaagacct actgaatacg ctcgacgtga
     2701 tcctgctggg cagcctgctc ggcgcctgcg tggtggtgat gctggcaacg ccctggctgg
     2761 tccggcgcgg gctgcgcccc atcgcggtgc tggatgacgc gatgaccggc atcggaccgg
     2821 acacgccggg tgcgcgcctg ctggcgagca ccagcaccga actcgcgcca ctggtcgtgc
     2881 gcatcaacga agtgctggcg cgcatggacg aaggactcgc gcgggaacgg caattcgcgt
     2941 ccgggctggc gcacgaattc cggacccggc tcgccgaatt gcgcacgttg gtggatgtcg
     3001 agacgcgtta tccgagtgga cgcgatgccc ggagcctcct ctgtgaagcc ggcagcatcg
     3061 gcgaggaact ggaagcaacc gtgaccgcgc tgctgcaact gacgcgcatc cagtcgggac
     3121 tggaacagtc gcaagccgaa agcgtgccgc tgcggccatt gttggcgcgg gtgcgcgcgc
     3181 gccaccagga tgctgcgcgc gcacgcggcg tgggcatcga acccggggac gcgtgcaaac
     3241 cggatttcac gatcaaggcc gatcccgtgc tgctggaaat cgtgctggac aacctgctcg
     3301 gaaacgcggt ggcgtacgtg ccggcgggtg gcatcgtcag cctgggcgcc agcgaaaacg
     3361 gcatcgaggt atgcaacgcc gcacccgcgc tgcatcccga agacctggtc aatttcggcc
     3421 ggcgcttctg gcgcaaggat gcgcagggcg cgggtcacgc ggggcttggc cttgcattag
     3481 ccgccgcagc cgccaaggcg ttgcgcatga cgttgtcgtt cgaattgaac gaaggcgtac
     3541 tacgcgcgaa cgtgcggtgg tgcacgaagg attaatcgcc acaggtcggt caggtgccgt
     3601 gacggcgcat cgccagcgaa taaagcaccg gcatcaccac cagcaccagc ggcaccgccg
     3661 tcagcatgcc cgcaatgatc gcgatcgcca gcggctgttg catctgcgag ccgcgtccga
     3721 tggccagtgc caacggggcg agcgtgagga tcgccgcgat ggtggacatc aggatcgcgc
     3781 gggtgcgccc gcggccggct tgaagcaaag ccttgcgcaa gggtggtgga ttgtcttcgt
     3841 ccgcccgagc ctggatgtcg gcgagctccg agaagaagaa aatcgacaac tcggtgacga
     3901 tgccgacgat catcgtcatg cccatcatcg atgaaatgtc caactcagtg ccggtgaccc
     3961 acaaaccgat gaataccccg cagatggcga ggatcggctg gaccagcacc accaccgccg
     4021 cgcgaaaact ttcgtacagg aacaagagca gcgtgaacac cagcgcgatc gcggtcagca
     4081 tcacgatggt gaggccgtgg aacgcaatct gctgttgctg atacaagccg cccagttcgt
     4141 agtacatccc cggcgggagt tcgccgcgcc ggctgagtac cctggtcacg tcgcgcatcg
     4201 tcgaacccag atcgcgtccg ctgatgcgcc cggtgaccgt gagcatgcgc ttcaggttct
     4261 cgtggtcgat ttccggctgc ccgctttgta tggtcacgct ggcgactcgc gacaggggaa
     4321 agacatggcc gtggccgtca tcgatgggaa gcgtggcgag ctgttcaatg gtcgtccggt
     4381 cgcgcggggg tatccacacg cgcacgccga ccatcttcgg gccttcgggg agttcggcgg
     4441 cgaccattcc ggccattgcc gtcgcgacct ggccggcgac gcttcgtgga tcgagtccca
     4501 gcagcgcggc ctgcaccgga tcgaccttca cttccagtcc gtcgccggcc gggttgatgc
     4561 cgttgcggac gctgaccacg ccagggatct tgccgagttg cgcagcgacc ttgttcgcgg
     4621 tggcatcgag ctcggtaaca ttgtcggaaa acagtttcac cgcaatcggc tgcggcaccg
     4681 cggtcaggtc gccgatcatg tcctcgagta actgcgagac gtccacgtcc agtcccggca
     4741 ccttggccgc gacctgttcg cggatgtgtt ccatcacctg ttcggtcggc ggccgcgcaa
     4801 cgttcttcag ccgtacgaac aggtcgccgc tgttggcttc ggtgaggcca ccgcccagtt
     4861 gcaggccggt gcgccgcgac caggtatcga cgtagggatc cgcggcgagg atgccatcga
     4921 ccttttgcaa caggcggtcg gtttccgcca gtgaggtgcc cggcagcgaa cggtagtcga
     4981 gggtgaatcc gccttcatcc atcggcggca tgaaacccga gcccaccgat ttgtaggcga
     5041 agaatcccaa caccagcagc gggatcagcc cgagcagcag cagcgcgggc cattccagca
     5101 cccgcagcag cacgctttcg tagaatccca tgaagcgttt ccacaggtgt gacggcggac
     5161 gctcccgggc gtgcgtcgcg ccgatcagcc attgcgccag cagcggcacg acgatcagcg
     5221 tcaacagcca ggaaatgacc agcgcgcttg ccatggtcaa cgacagtgcc ttgaaaaaag
     5281 cgccggtgac acccgtcagg aacgccagcg gcaggaagat cacgatggtg gcaagcgacg
     5341 aaccggtttg cggccgggtg aactcgtgcg cggccgacat gatgcgttcg cggacatgac
     5401 cggattcgct cacgacccgt cgcatgatgt gttcggtcat taccaccacg tcgtcgatca
     5461 gcaggccgac cgcggcggcc atgccgccca gcgtcatcat gttgaagctc atgtgcagga
     5521 tcttgagcag cagcaccgtg atcgcgaggg tggccggcac caccagcatc gcgatcagga
     5581 tcacccgcgt gttgcgcaga aacacgaaca acaccagccc cgcgagcacg atgccgatca
     5641 ggatcgcgtc gcgcacgctg cccgccgatt cccgtaccag ctgggtctgg tcgtaccacg
     5701 ccgcgatctc cacgcccttg ggcatctgcg gtgtataaga cgccagtttc gccttcaccg
     5761 catcgaccat cgcgatgctg ttggcagccg gctgctggaa cacctgcatc aggatcgcgg
     5821 gtttgccgtt ggcaccggtc atcacccact cgggtttgac gccgcgcgtc acggttgcaa
     5881 tgtcgccgac ccggactacg ccgcgcggat ccgcacgcac caccacatcg tcaagcgcct
     5941 gcacgctggc cggttggttg ttggccagaa ccaggaacag cttgtagtga tcggccaggc
     6001 ggccgagtgc cgagatgtcc gccgcgctgc cgaccgcacg cacgacgtcg gccatgccca
     6061 ggccctgcgc gcgcagtttg gccggatcga cattgacgtg gagctcggcg gtgccgcggc
     6121 cttgcacgtc aaccgtcgcg acgcccggca cgcccgagat cagcgggcgc aactggtatt
     6181 gggcgatgtc gtacaactgc gttggcgtca gcgttttcga acgcagactg taggcgatca
     6241 ccggctcgtc cgcggtcggg tccatgcgta tggcctggat tcgcgtgccg gatggaagat
     6301 ccggcagcac ctggcttgcc gcggcgttga cttcgaggaa cgcgcgcgcc atgtctgcgc
     6361 cccagtcgaa atccacgttg atttcggcac tcccgcggct ggtggtcgaa cggacattgc
     6421 gcacgccgcg cacttggcgg atcgcctctg ccaccgggcg tgtcacggcg acggccatct
     6481 ggtctgcggg gcggtcgccg gcgtcgatcg acacgcgtat ccgcggaaac gaaacgtcgg
     6541 ggaacagcgc aaccggcaga ttgaacagcg ataccaagcc gcccaacgcc aggatcacca
     6601 ccaggaacag cagcgaccgg cggtgcacct ggaaccaagt ggcgatgttc atcgtgcggc
     6661 ggttccgtgc ggcgcgctcg tggccggtgc cgatgcgcgc acgggatcgc cgtcggaaac
     6721 ctcgtaactg cccaatgtga tca
//
LOCUS       DASSNH010000118         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_18460, whole genome shotgun sequence.
ACCESSION   DASSNH010000118 DASSNH010000000
VERSION     DASSNH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18460"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFK00_04225"
     CDS             complement(<1..543)
                     /locus_tag="VFK00_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7592196.1"
                     /translation="MNERVDLLIVGGGLVGASLALALDAAGRSVTLVEAMTSRGSGAA
                     GDDDRNLVLARASVNALRSLGVWPRLEAAAGTIRHLEISRGGEFGRVRLSAEDAGVDA
                     LGRVVPGRVLASALEARLAEARHVQRLVPAALESLTPTDSGWLARIAIGGEVREIEAR
                     LVVGADGSASMVRRQVGIEAQ"
     gene            695..1309
                     /locus_tag="VFK00_04230"
     CDS             695..1309
                     /locus_tag="VFK00_04230"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HET7592197.1"
                     /translation="MSQALPSRAPQSTIFTPGTLVLCGMVAFTVIYRLLVHFAAGALP
                     WNFTPVEATALFGGAYFADRRLGVAIPLIAMIIADCVIAFTLPTAWVRDWLGTLPFVY
                     ICIVMTAVGGFALRGKVSAPRVAIGAVTSAVLFFLVTNFATWLTARPDAGAACTGTLT
                     ACYVAGIPFFKGTLYGALFWSALLFGGFALLGRRWNVLRLTAAH"
     gene            1331..1891
                     /locus_tag="VFK00_04235"
     CDS             1331..1891
                     /locus_tag="VFK00_04235"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7592198.1"
                     /translation="MGNRLSKIYTRTGDDGTTGLGDGSRTPKDSARVESYGTVDELNS
                     AIGVLLACEGVPDDVREHLVRIQHALFDLGGELCVPGMELVHDADVTRLEEILDDYNA
                     ELPRLKEFILPGGGPAAAQCHVARTVCRRAERRVVTLAREESVRPEVVRYLNRLSDLL
                     FVFARVLARASGHGEVLWQHERRKQS"
     gene            1921..2121
                     /locus_tag="VFK00_04240"
     CDS             1921..2121
                     /locus_tag="VFK00_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592199.1"
                     /translation="MRMVKGFLAIASILFLLIALLHALRFQEWVVSAHYGVGAVLFWL
                     ATVLVPLALSLWGLASFRRIGR"
     gene            2124..3041
                     /locus_tag="VFK00_04245"
     CDS             2124..3041
                     /locus_tag="VFK00_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET7592200.1"
                     /translation="MHIYTHPACLEHDPGYGHAESPARLRAVLQALDDPCFSTLERVE
                     APRATREQLQRVHDADYVDSILSGAPAQGFVRLDPDTVMSPGSTEAASRAAGAVCAAT
                     DAVLRGETRRAFCAVRPPGHHATRDQAMGFCLFNNVAVAAAHALAVHGIERVAIVDFD
                     VHHGNGSQDIFATDPRVLYASTHQVPLYPGTGAAAEHGAGNIFNAPLPPEAGSGEFRA
                     TWEKRLLPELDLFRPQLLLVSAGFDADARDPLAQLRLVPDDYAWITRRLVELADRHAD
                     GRIVSALEGGYDLDALREDVAAHATALMG"
     gene            3085..3456
                     /locus_tag="VFK00_04250"
     CDS             3085..3456
                     /locus_tag="VFK00_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7592201.1"
                     /translation="MAARTGMLVPYAHVADVERSIGFYAGLGFRVKNTVVPDGQTAPV
                     WAWLDSEKANLMVGLASGPIDSGQQAVLFYLYYDDIKQTHAELTALGYAVGGIKYPFY
                     MPGGEFRLEDPDGYVLMLAQI"
     gene            complement(3644..>5312)
                     /locus_tag="VFK00_04255"
     CDS             complement(3644..>5312)
                     /locus_tag="VFK00_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET7592202.1"
                     /translation="QRENQTMATITFQNLFRMYDKLSGMTGTADTEAFEFQSIYNLDV
                     VVIPTNKPMVRKDNADLVFLKPEPKFKAVLEDIRECRERGQPVLVGTASIEVSELVSG
                     LLKKAGVAHEVLNAKQHEREAKIIEQAGAPGAVTIATNMAGRGTDIVLGGSLDAALAD
                     LPPDATDADKERVKAEWKKRHEQVVAAGGLHIIGTERHESRRVDNQLRGRSGRQGDPG
                     SSRFYLSLQDNLMRIFGGAGLVRWMQRFGMKEDDALEDRLVSRQIEKAQRKVEAHNFD
                     IRKQLLEFDDTANDQRKVIYEQRRELLESDDVADTVTDIRRDVFESLASRYVPPESID
                     DQWDLAGLQATLESDFGMKLDLRKWLETDQEADAAAIHQHVIEAADTMFAAKEQQVGA
                     EVMRALEKHVMLTVVDSAWKDHLASMDYLRQGIYLRSYAQVDPKKEYKREAFRLFEDM
                     LERIKTELVQTLARVRIRSEEEVAALEAEQRRAAEARAMEFQHAQSTGYDAPPQPGAE
                     AGQPQQGAEGAVATATPMVRSGPKIGRNDPCPCGSGKKYKHCHGALT"
BASE COUNT          760 a         1845 c         1746 g          961 t
ORIGIN      
        1 ctgcgcctcg atgccgacct gccggcgcac catcgaagcg ctgccgtcgg ccccgaccac
       61 caggcgcgcc tcgatttccc gcacctcgcc gccgatcgcg atgcgcgcca gccaacccga
      121 atccgtggga gtgagcgatt ccagtgccgc cggcaccagc cgctgcacgt gccgggcttc
      181 ggccaagcgc gcttccaatg cggaggcgag tacccgtccg ggcaccacgc gcccgagcgc
      241 atcgacaccc gcatcctcgg cggacaggcg cacccgcccg aactcgccgc cgcggctgat
      301 ctcgaggtgg cggatggttc cggccgccgc ttcgagccgc ggccacaccc cgagcgatcg
      361 cagcgcgttc accgacgcac gcgccagcac caggttgcgg tcatcgtcgc ctgctgcacc
      421 gctgccgcgg gacgtcatcg cttcgaccag cgtcacgctg cgaccggcgg catcgagtgc
      481 cagcgccagg ctcgcgccga cgaggccgcc cccgacgatc agcaggtcca cccgctcgtt
      541 catcgcatga tgatacccgg ttgatgcggg tcagcccgcg ctccgcgtac gttgcggtaa
      601 gctagtgcaa tcagccgtcc ggcacgcgcc gcatctttcc tgcgcgcccc tccccggcgg
      661 cttcgccacc gcatcgaaca agaggatgga ttccatgtcg caagccctgc cgtcccgcgc
      721 cccgcaatcg accatcttca cccccggcac gctggtgttg tgcggcatgg tcgccttcac
      781 cgtcatctac cggctgctgg tgcattttgc ggcgggcgcg ctgccatgga acttcacccc
      841 ggtggaagcc acggcgctgt tcggcggcgc ctattttgcc gaccgccgcc tcggcgtcgc
      901 gattccgctg atcgcgatga tcatcgcgga ttgcgtcatc gccttcaccc tccccacggc
      961 gtgggtgcgc gactggctgg gcacgttgcc attcgtgtac atctgcatcg tgatgaccgc
     1021 cgtcggcgga ttcgccctgc gcggcaaggt cagcgcgccg agggttgcca tcggcgccgt
     1081 cacgagcgcg gtgctgttct tcctcgtgac caactttgca acctggctca ccgcccgccc
     1141 cgatgccggc gcggcgtgca ccggtaccct caccgcctgc tacgtcgccg gcattccgtt
     1201 cttcaagggc acgctgtacg gcgcactctt ctggagcgcg ttgctgttcg gcggcttcgc
     1261 cttgctcggg cggcgctgga acgtgctgcg gctgaccgcg gcgcactgat cgcccggcgg
     1321 tcgcgttgcc gtgggcaacc gcctttcgaa gatctacacc cgcaccggtg atgacgggac
     1381 cacgggcctc ggcgatggtt cccgcacacc caaggattcg gcgcgggtcg aatcgtacgg
     1441 cacggtagac gaactgaatt ccgcgatcgg cgtactgctc gcctgcgagg gcgtgcccga
     1501 cgacgtgcgc gagcacctcg tccgcatcca gcacgcgctg ttcgatctcg gcggcgaact
     1561 ctgcgtgccc gggatggagc tggtccacga cgccgatgtc acccggctcg aagagatcct
     1621 cgacgattac aacgccgaac tgccgcggct caaggaattc atcctgcccg gcggcggccc
     1681 ggcggccgcc cagtgccacg tcgcgcgcac cgtctgccga cgcgccgagc ggcgggtggt
     1741 gacgctggcg cgcgaagaga gcgtgcggcc cgaagtcgtg cgttatttga atcgcctgtc
     1801 cgacctgctg ttcgtgttcg cgcgcgtgtt ggcccgcgcc agcgggcacg gcgaggtcct
     1861 gtggcagcac gagcgcagaa agcaatcctg aatcgctggc cggtccgcga ggagaacggt
     1921 atgcgcatgg tgaaggggtt cctggcgatc gcttccatcc ttttcctgtt gattgcactg
     1981 ctgcacgcat tgcgattcca ggaatgggtc gtatccgcgc actacggcgt gggagccgtg
     2041 ctgttctggc tggcgacggt gctcgtgccg cttgccctga gcctgtgggg gcttgcgtcc
     2101 ttccgcagaa tcggtcgatg accctgcaca tctacaccca tcccgcctgc ctcgaacacg
     2161 accccggcta cggccatgcc gagtcgcccg cccgcctgcg cgcggtactg caagccctgg
     2221 acgacccctg cttctcgaca ctggaacgcg tcgaagcgcc acgcgcgacg cgcgagcaac
     2281 tccagcgcgt gcacgacgcc gactacgtgg acagcatcct ttcgggcgcg cccgcgcaag
     2341 ggttcgtgcg actcgatccc gacaccgtga tgtcaccagg gtccacggaa gccgcgtcgc
     2401 gagcggccgg tgccgtgtgc gcagcgacgg acgccgtgct gcgcggcgaa acgcggcgcg
     2461 cattctgcgc cgtgcgcccg cccggccatc atgcgacccg cgaccaggcc atggggttct
     2521 gcctgttcaa caacgttgcg gtcgccgcgg cgcatgcact ggccgttcac ggcatcgaac
     2581 gcgtggccat cgtcgatttc gacgtgcacc acggcaacgg cagccaggac atattcgcga
     2641 ccgatccgcg cgtcctgtac gcgtccacgc accaggtgcc gctgtacccc ggcacgggcg
     2701 ccgcggccga gcacggtgcc ggcaacatct tcaacgcacc gttgccgccc gaggccggtt
     2761 ccggcgaatt tcgtgcgacc tgggaaaagc gcctgctgcc cgagctcgac ctgttccgcc
     2821 cgcaactgct gctggtttcc gccggcttcg acgccgacgc acgcgacccc ctggcgcaac
     2881 tacgcctcgt ccccgacgac tatgcgtgga tcaccaggcg cctcgtcgaa ctcgccgatc
     2941 ggcatgccga tggacggatc gtctccgcgc tcgaaggcgg gtatgacctc gatgcgctgc
     3001 gcgaggatgt ggctgcgcac gccacggcat tgatgggttg aaccatactg gcgcgtgccg
     3061 ctgatcccaa gcgtcggagg tcacatggca gctcgaacag gaatgctggt gccgtacgcc
     3121 cacgtcgctg acgtcgaacg cagcatcggg ttctatgccg gcctcgggtt cagggtgaaa
     3181 aacaccgtcg ttcccgacgg acagacggcg cctgtgtggg cctggctcga cagcgaaaag
     3241 gccaatctga tggtgggact cgcgtcgggg cccatcgatt cgggtcagca agccgtgctg
     3301 ttctatctgt attacgacga catcaagcaa acgcatgccg agctgacggc gctgggctat
     3361 gcggtgggcg ggatcaagta cccgttctac atgccgggtg gggagttcag gctcgaggac
     3421 cccgatgggt acgtgttgat gctggcgcag atttaggggc ggcttgcgcg ctgcgcgcgg
     3481 cccggaagga cgattgttgg ttgggacgtt tcacgggcgc gtaccctcac ctgcccttcg
     3541 ggcaccctct cccgggggga gagggaagag ccatccccgg cgcttcgcgc gccccgtggg
     3601 caagcgaatg ggtgtggatc ccggcctgcg ccgggatgac ggcctacgtc agggcgccgt
     3661 ggcagtgctt gtatttcttg ccggagccgc aggggcaggg atcgttgcgg ccgatcttgg
     3721 ggcccgagcg caccatcggg gtggctgtgg cgacggcgcc ttcggcgcct tgctgcggct
     3781 gcccggcttc cgcgcccggt tgcggcgggg cgtcgtagcc ggtcgattgc gcgtgctgga
     3841 actccatggc gcgggcctcg gcggcgcggc gctgctcggc ttccagcgcg gcaacttcct
     3901 cttcgctgcg gatgcgcacg cgcgccagcg tctggacgag ttcggtcttg atgcgctcga
     3961 gcatgtcctc gaacagacgg aacgcctcgc gcttgtattc cttcttcgga tcgacctgcg
     4021 cgtaggaccg aaggtaaatg ccctgccgca ggtaatccat gctcgcgagg tggtccttcc
     4081 acgcgctgtc gaccacggtc agcatcacgt gcttttcgag cgcgcgcatg acctcggcgc
     4141 cgacctgctg ctccttggcg gcgaacatgg tgtcggcggc ctcgatcacg tgctggtgga
     4201 tcgctgcggc gtcggcttcc tggtccgtct ccagccactt cctgaggtcg agcttcatgc
     4261 cgaagtcgga ctcgagcgtc gcctgcagcc ccgccaggtc ccactggtcg tcgatgctct
     4321 ccggcggcac atagcggctt gccagcgact cgaacacgtc gcggcggatg tcggtcaccg
     4381 tgtcggcaac gtcgtcggat tcgagcagtt ccctgcgctg ctcgtagatc accttgcgct
     4441 ggtcgttggc ggtgtcgtcg aactccagca actgcttgcg gatatcgaag ttgtgggcct
     4501 cgaccttgcg ctgcgccttc tcgatctgcc ggctgacgag gcggtcctcc agcgcgtcgt
     4561 cctccttcat gccgaagcgc tgcatccagc ggaccaggcc ggcaccgccg aagatgcgca
     4621 tcaggttgtc ctgcagcgac aggtagaagc gcgaggagcc cgggtcgccc tggcgcccgg
     4681 agcggccgcg caactggttg tcgacacgcc gcgattcgtg gcgctcggtg ccgatgatgt
     4741 gcaagccgcc ggcggccacc acctgctcgt ggcgcttctt ccactcggcc ttgacccttt
     4801 ccttgtcggc atcggtggca tcgggcggga ggtccgccag cgccgcgtcg aggctgccgc
     4861 cgagcacgat gtcggtgccg cggcccgcca tgttggtcgc gatggtgacg gcacccgggg
     4921 cgccggcctg ctcgatgatc ttggcctcgc gttcgtgctg cttggcgttc agtacctcgt
     4981 gggcgacgcc agccttcttg agcaggcccg acaccaattc ggagacttcg atcgaagcgg
     5041 tgccgaccag caccggctgg ccgcgctcgc gacattcgcg gatgtcttcc aggaccgcct
     5101 tgaacttggg ttcgggcttc aggaacacga ggtccgcatt gtccttgcgg accatcggct
     5161 tgttggtcgg aatcaccacc acgtccaggt tgtagatcga ctggaactcg aacgcttccg
     5221 tgtccgccgt gccggtcatg cccgacagct tgtcgtacat gcggaacagg ttctggaagg
     5281 tgatggtggc catcgtctgg ttttcgcgct gg
//
LOCUS       DASSNH010000119         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_7909, whole genome shotgun sequence.
ACCESSION   DASSNH010000119 DASSNH010000000
VERSION     DASSNH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7909"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFK00_04260"
     CDS             complement(<1..254)
                     /locus_tag="VFK00_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET7592203.1"
                     /translation="MSRWRPPPARSTAIITRAGFDALKSELDHLWRELRPEVVKALAA
                     AAAEGDRSENAEYTYRKKQLGEIDRRVRYLSKRIPSLKVA"
     gene            462..1946
                     /locus_tag="VFK00_04265"
     CDS             462..1946
                     /locus_tag="VFK00_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7592204.1"
                     /translation="MMRVVQMLGRSGWLLCAGALGLLAGCAQAPTHGAGSPAELNALY
                     SQLDQASAQYDQALMQARRGENAQSSKTLEAALDAMRTASDTCVNTSGCDPQRFLSAY
                     DRALRLKDGSFLGTENDQDVLDAQDQTAAGADSGVLATLPQAQRTVQLLHGHTLSDLV
                     ATNGSVQAALQVWLTRLRPNLIRAYVDYQYLRYQMWPAFHKAGLPEAILFGVMAKESG
                     GRVHAMSRSGAAGPLQFMPATGARFGLDTINGFDQRFDPTMAAQAAASYFDEQLGRLN
                     DDLALVLAAYNGGEGRVGRLTAGVQDASFWNPDVYFSLSAETREYVPMVLAAAWLFLH
                     PERYNLRFPKVDGAPGHVDLARAASLNELSVCLGNDDDAHYGWYRTLRNLNPQLDASD
                     VQPEGTRISLPRFLEGAYQRSCVSGKWVALADQLQNAQLPERVAADPPAPSHYTVRRG
                     DTLSAISQRFHCDGVRELARSNHLRSASSLQIGKRLRLEGCSRR"
     gene            2126..2216
                     /locus_tag="VFK00_04270"
     tRNA            2126..2216
                     /locus_tag="VFK00_04270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2160..2162,aa:Ser,seq:cga)
     gene            2278..3948
                     /locus_tag="VFK00_04275"
     CDS             2278..3948
                     /locus_tag="VFK00_04275"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7592205.1"
                     /translation="MRELRWTIVLLAGLAVPAAGSAQAAASSASKPATSGSATYYASI
                     AELQQGMRGGSFDSADLVRSFIQRIDELDQAGPRVNSVIELNPDALSIAKQRDEGREP
                     SPRRNLWGIPVLLKANIDTGDKMPTTAGSLALLGAPAPRDATVAAKLRDAGAVILGKT
                     NLSEWANFRSTNSSSGWSGRGGLTRNPYVLDRSACGSSSGSAAAVAAGFTTVAIGTET
                     NGSIICPAAANGIVGIKPTVGLVSRAGIVPISSTQDTAGPMARDVRDAAIVLSALAGS
                     DPRDPATADADRHATDYTKFLDADALRGKRIGVVRALADDGPGAAKLLDQAIAVLRSR
                     GATVVDPLKIPHLHDYGKAEMTVLLYEFKHGINAYLATRRGLSVHSLADLIAWDKAHA
                     AEEMPWFGQELFIEAEAKGPLDDKAYRDALAKLQKLAGPEGIDAAMDANHLDALLVPA
                     QPPSWPIDLVNGDASLPSGYGPAAVAGYPSITVPSGLVHGLPVGMLFFGRKWSEPTLV
                     GIAYAYEQATHARRRPQFLPTLPAPEGIDPYTGKSAASASPSQADTSH"
     gene            complement(3992..4690)
                     /locus_tag="VFK00_04280"
     CDS             complement(3992..4690)
                     /locus_tag="VFK00_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7592206.1"
                     /translation="MKILMVLTSHGQLGKTDKSTGLWLEELAAPYYVFKDAKAEVTLA
                     SPAGGQPPVDPSSEKPDNESDATRRFRADTEAIQALANTRKLADIEAGAYDAVFFPGG
                     HGPLWDLAEDDDSRRLIETLYAAGKPVAAICHGPAALRHARGPYGLPLVRGKAVTGFS
                     NSEEQAAGLADAVPFLIEDMLKEAGGEYSKAANGQSHVVVAGNLVTGQNPASSAEAAK
                     AVLQQLRGQTAKAA"
     gene            4816..>5138
                     /locus_tag="VFK00_04285"
     CDS             4816..>5138
                     /locus_tag="VFK00_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HET7592207.1"
                     /translation="MGLISAPISHGELIDKLTILEIKSERMADAAKLANVRDELQLLS
                     SLWQADAASRTDIAAERAELKRINEALWEIEDEIRVKERDQAFDARFIELARAVYHTN
                     DRRAAV"
BASE COUNT          828 a         1701 c         1747 g          862 t
ORIGIN      
        1 tcggccactt tcagcgacgg gatgcgcttg gagagatagc gcacccggcg gtcgatttcg
       61 ccgagctgct tcttgcggta ggtgtattcg gcattctcgg agcggtcgcc ttcggcggct
      121 gccgcggcca gcgccttcac cacctccggg cgcaattccc gccacagatg atcgagctcg
      181 gatttcagcg cgtcgaaccc cgcgcgcgtg atgatcgcgg tcgaacgcgc ggggggcgga
      241 cgccagcggc tcatgcgacc cggtccatgc cggctgcgaa ccggccgact tccgccacct
      301 ggtggggcac ggcggcggtg tcgcccgtgc cgatcaggat gttgcccaag gccgccccca
      361 ttcgtgtacg cggtcacgcg attgtacgtg gcacgctccg gggtgtgcgc tgctaccttg
      421 agtcgccctg tgcttcccgt tagggtcggc gggaattcga aatgatgcgg gtggtgcaga
      481 tgttggggcg atcggggtgg ctgttgtgcg cgggggctct ggggttgctg gcgggttgcg
      541 cgcaggcgcc tacccacggc gccggcagtc cggccgaact gaacgcgctg tactcgcagc
      601 tcgaccaggc cagcgcccag tatgaccagg cgctgatgca ggcacgacgc ggcgagaacg
      661 cacagtcgag caagaccctc gaagcagcac tggatgccat gcgcaccgcg tcggacactt
      721 gcgtcaatac gtccggatgc gatccccagc gattcctgtc ggcctacgac cgggccctgc
      781 gcctcaagga cggcagcttc ctcggcaccg agaacgacca ggacgtgctc gacgcccagg
      841 accagaccgc ggccggcgcg gattccggcg tgctggccac gttgccgcag gcgcagcgca
      901 cggtgcaact gctgcacggc cacacgctct ccgacctggt ggccaccaat gggtcggtgc
      961 aggcggcgct gcaagtgtgg ttgacccgcc tgcgccccaa cctgatccgc gcctacgtgg
     1021 actaccagta cctgcgctac cagatgtggc cggcgttcca caaggcgggg ctccccgagg
     1081 ccatcctgtt cggagtcatg gcgaaggagt ccggcggcag ggtccacgcg atgtcgcgct
     1141 ccggcgcggc gggtcccttg cagttcatgc cggccaccgg tgcgcgcttc ggcctcgaca
     1201 cgatcaatgg tttcgaccag cgcttcgatc cgaccatggc ggcgcaggcg gctgcgtcgt
     1261 acttcgacga gcagctcggc aggctcaacg acgatctcgc cttggtgctg gccgcctaca
     1321 acggtggcga gggcagggtg gggcggctga ccgccggcgt gcaggacgcg agcttctgga
     1381 atccggacgt ctatttctcg ttgtcggcgg agacccgcga atacgtgccg atggtgctgg
     1441 ccgcggcatg gctgttcctg cacccggaac gctacaacct gcgctttccg aaggtggacg
     1501 gggcgcccgg ccatgtcgat ctcgccaggg ccgcgtcgct caacgaactg tccgtgtgcc
     1561 ttggcaacga cgacgacgcg cattacggct ggtatcgcac cctgcgcaac ctcaatccac
     1621 aactcgacgc gtcggacgtg caacccgagg gcactcgcat cagcctgccc aggttcctgg
     1681 agggcgccta tcagcgctcg tgcgtcagcg ggaaatgggt cgcattggcg gaccagttgc
     1741 agaacgccca gttgccggaa cgggtggccg ccgatccgcc ggcgcccagc cattacaccg
     1801 tgcgccgcgg cgacacgctg tccgccatct cgcagcggtt ccattgcgac ggcgtccgcg
     1861 aactcgcccg gagcaaccac ctccgttcgg cctccagcct ccagatcggg aagcggctgc
     1921 ggctggaggg gtgcagtcgg cgctgacgcc cggcgagtcg tcccccgggg ctggtttcca
     1981 gtgcgggctt ggggatccga accggagcga gtggcgcgcc gcttcgtatc gccccttcgc
     2041 acttagcccg gatgcgcagc tcgtattggc agcacgccgg accgccgtta tcatttgcgg
     2101 tccgctcacg ctttcgcgga cacccggaga ggtggcagag tggtcgaatg cgccggattc
     2161 gaaatccggt ttacggtatt gccgtaacgt gggttcgaat cccaccctct ccgccagaaa
     2221 aaaggaacgg ggccgctggc cccattcttg tttctgacga acctgaagga aggcaccatg
     2281 cgcgaattgc ggtggacgat cgttctcctt gcgggccttg cggtgccggc ggcgggatca
     2341 gcgcaagcag cggcatcttc ggccagcaaa ccggcaacct ccggatcggc aacgtactac
     2401 gcgagcatcg ccgagttgca gcaggggatg cgcggcggct cgttcgattc ggcggacctg
     2461 gtccgtagct tcatccagcg catcgacgag ctcgaccagg ccgggccgcg cgtgaactcc
     2521 gtgatcgagc tgaatccgga tgccctgtcg atcgcgaaac agcgcgacga agggcgcgag
     2581 ccgagtccac gtcgcaatct ttggggcatt ccggtactgc tgaaggccaa catcgacacc
     2641 ggcgacaaaa tgccgaccac cgcgggttcg ctggccttgc tcggcgcccc ggcgccccgc
     2701 gacgccacgg ttgcggcgaa actgcgcgac gcgggcgccg tgatcctcgg caagaccaac
     2761 ctgagcgagt gggccaactt ccgttccacg aactccagca gcggctggag tggtcgcggg
     2821 ggccttacgc gcaatccgta cgtgctcgat cgctccgcct gcgggtcgag ttccggatcg
     2881 gccgcggcgg tcgcggcggg cttcaccacg gttgcgatcg ggaccgaaac caatggctcg
     2941 atcatctgcc cggcggccgc caacggcatc gtcggcatca agccgacggt gggcctggtg
     3001 agccgcgcgg ggatcgtgcc gatcagctcg acccaggaca cggccggacc gatggcgcgc
     3061 gacgtgcgtg atgccgcgat cgtgctgtcg gcactcgcgg gcagcgatcc gcgtgacccg
     3121 gcgacggcgg atgcggaccg ccatgcgacc gactatacga agttcctgga cgccgatgcg
     3181 ttgcgcggca agcgcatcgg cgtggtgcgc gccctcgccg acgacgggcc cggggccgcg
     3241 aagctgctgg accaggccat cgcggtgctg cggtcgcgcg gcgcaacggt ggtcgatccc
     3301 ctcaagatcc cgcacctgca cgattacggc aaggccgaga tgaccgtgct gttgtacgaa
     3361 ttcaagcacg gcatcaacgc atatctcgcc acgcgcaggg gtttgtcggt gcattcgctg
     3421 gcggatctga tcgcctggga caaggcgcac gcggcagagg aaatgccctg gttcggccag
     3481 gaactcttca tcgaggccga ggccaagggt ccgctggatg acaaggccta tcgggacgcg
     3541 ctggccaaat tgcagaagct ggccggaccg gaaggcatcg acgccgccat ggacgccaat
     3601 cacctggatg ccttgctggt gccggcgcag cccccgtcgt ggccgataga cctggtgaac
     3661 ggcgatgctt cattaccgtc tggctacggt cccgccgcgg tggccggata tccgtccatc
     3721 acggtaccgt cgggactcgt gcacgggttg cccgtgggga tgctgttctt cgggcgcaag
     3781 tggagcgaac cgaccctggt cgggatcgcc tatgcgtacg aacaggccac ccacgcccgg
     3841 cgccggccgc agttcctgcc gacgctgccg gcgcccgagg gcatcgaccc gtacaccggg
     3901 aagtcggctg cttccgccag cccctcgcaa gccgatacat cgcattgaat cgcggccggg
     3961 cacgcggatg gcgtgcctga tcaagcgaat ctcacgcagc cttggccgtc tggccgcgca
     4021 gttgttgcaa caccgccttc gccgcctccg cggaggacgc gggattttgc ccggtgacga
     4081 ggttgccggc gacgacgacg tgcgactgtc cgttggccgc cttggagtac tcgccaccgg
     4141 cctccttcag catgtcctcg atcaggaacg gcacggcatc ggcgaggccg gctgcttgtt
     4201 cctcggagtt ggagaagccg gtgacggcct tgccgcgcac gagcggcaat ccgtagggtc
     4261 cgcgcgcgtg acgcagcgcg gccgggccgt ggcagatcgc ggcgaccggc ttcccggccg
     4321 cgtaaagcgt ttcgatcaac cgccgagaat cgtcatcttc cgccaggtcc cacagcgggc
     4381 cgtggccgcc ggggaagaac accgcgtcgt aggcgccggc ctcgatgtcc gccaacttgc
     4441 gggtgttggc gagcgcctgg atggcttcgg tgtccgcacg gaaccgccgg gtggcatccg
     4501 attcgttgtc gggcttttcg ctactggggt cgacgggcgg ctgcccaccg gcgggcgacg
     4561 ccagggttac ttcggccttg gcgtccttga acacgtaata gggcgcggcc agttcttcca
     4621 gccacaggcc ggtcgacttg tcggtcttgc caagctggcc atgcgaggtc agcaccatca
     4681 ggatcttcat ggtcgtactc cggttcaccc gttgcgaggg gcttcactgc acaatgggaa
     4741 cggtcccggc gaatgcaagg gagggtgccg ttggggcggt gttacgctgc gcgtaacttt
     4801 tcagcgagtt ggcccatggg actgatttcc gcaccgatct cccatggtga gttgatcgac
     4861 aagctcacca ttctcgaaat caagtctgaa cgcatggcgg acgcagccaa gctcgccaat
     4921 gtccgcgacg aactgcagtt gctgtcgtcc ctgtggcagg cagacgccgc gtcgcggacg
     4981 gacatcgccg cggaacgggc cgagctgaag cgcatcaacg aagcgctttg ggagatcgag
     5041 gatgaaatcc gcgtcaagga gcgcgaccag gcgttcgatg cgcggttcat cgaactggcg
     5101 cgcgccgtct accacaccaa tgacaggcgc gccgcggt
//
LOCUS       DASSNH010000120         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_889038, whole genome shotgun sequence.
ACCESSION   DASSNH010000120 DASSNH010000000
VERSION     DASSNH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889038"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="VFK00_04290"
     CDS             <1..1957
                     /locus_tag="VFK00_04290"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET7592208.1"
                     /translation="KAAIRELQGKGFALPDYPDEPKNDAERETKARYDKVKGSAVNPV
                     LRQGNSDRRAPPPVKDYARKHPHRMGKWSGDSKTHVAHMEANDFYGTEKSATLARAGS
                     LRIVLEGADGESRILKDQIKVKEGEIVDAAVMSRRALATFIDAQIASARGSGVLFSVH
                     FKATMMKVSDPIMFGVVVEEFFRDVLALHGDALKQAGFNPNNGLGDLYACLAKLDDDQ
                     QHAIKADIEAQYAQRPQLAMVDSDKGITNLHVPSDVIIDASMPAMIRDSGKMWNADGE
                     RQDCKAVIPDRSYAGIYEVMIEDCKANGAFDPATMGSVPNVGLMADKAEEYGSHDKTF
                     QIPVDGAVRVVDQDGQAIFEHEVEAGDIWRMCQTKDAPVADWVKLAVSRARLSNTPAV
                     FWLDRNRAHDAEIIKKVERYLEDHDTSGLHIQILAPEDAMRHSLERIRDGEDTISVTG
                     NVLRDYLTDLFPIMELGTSAKMLSIVPLMAGGGLFETGAGGSAPKHVQQFVAEDYLRW
                     DSLGEFLALGASLEHLARTYDNARAKVLAAALDQAIGRFLETNKSPARKIGEIDNRGS
                     HFYLAMYWARALADQKDDAGLAKPFGKLADELEAGEACIVAELNGVQGEPVDLGGYYR
                     PDMAKVRAAMRPSKTFNDALEALRREV"
     gene            2113..2742
                     /locus_tag="VFK00_04295"
     CDS             2113..2742
                     /locus_tag="VFK00_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592209.1"
                     /translation="MAAWGTITMGRMAIGRMDEWPAWQRWGALVLAWALYIGGFWLLF
                     ATHGLAVRGLALLVLLAGLLLLVYASKAVLNERVRSIDRWQMRAALPAFLVYILLALY
                     VLPLVDQITTPWLKAAAALSPMAPLLFVAWVMVRYVNRCDEMERRQQLEAAGVAVLVV
                     GLLGMSLGLLSAAKLVVIDGTFALLMVLPALAVVYGLACVLAKWRHRAR"
     gene            2739..2939
                     /locus_tag="VFK00_04300"
     CDS             2739..2939
                     /locus_tag="VFK00_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7592210.1"
                     /translation="MKNRVRELREAHGWSQGALAELLDVSRQTINAIENGKYDPSLPL
                     AFKLARLFKQRIESMFEPDEGR"
     gene            2940..>4390
                     /locus_tag="VFK00_04305"
     CDS             2940..>4390
                     /locus_tag="VFK00_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7592211.1"
                     /translation="MKMNALKLNARVLLRIAAIVIAAAVIGWQWWGRDHARAPAADEA
                     GSLAATASAPPVPPAVAPAFTAPRKPATPEKLAMGKLTLTACQLKQPHSAVTTPAFCA
                     KFPVPENRADPGSRTIELKLAVIKSDSAVPARDLVVYLAGGPGQSAIETYPEMAPAFA
                     PLLKHHDLLLLDQRGTGGSHPLTCPDVEKELDKFTDAAPDPARMTEMIGKCAAEVEKT
                     ADPRYYTTTDAVADLEAVRQALGAPELDLVGVSYGTRMAQHYAGAHPDAVRSIVLDGV
                     VPNQLVLGESFAEALEHSLKLQSEACNAAPACKKRFGNWLATLHQLHAKLETAPAQQV
                     TFADPYSYKPVTRKLSADTLAGVVRFFSYSAATAALLPLAVDEAAHGNYAPLMGQAKL
                     LTSGVSQSMQGGMQFSVVCSEDASLLEPRPQDKDTILGSSMIDDLLAACKAWPHAATP
                     KDFHAPFRSSIPTLLISGERDPVTPPSNANEVL"
BASE COUNT          778 a         1424 c         1507 g          681 t
ORIGIN      
        1 gaaggcggcg atcagggaat tgcagggcaa gggcttcgcg ctgcccgatt atcccgacga
       61 gccgaagaac gatgccgaac gggagacgaa ggcgcgctac gacaaggtca agggcagcgc
      121 ggtcaatccc gtgctgcgcc agggcaactc ggaccgccgt gcgccgcccc cggtgaagga
      181 ttacgcgcgc aagcacccgc acaggatggg caagtggagt ggcgattcca agacccacgt
      241 cgcgcacatg gaggcgaacg atttctacgg caccgagaaa tccgcaaccc ttgccagggc
      301 cggcagcctc aggatcgtgc tcgagggtgc cgatggcgaa tcgcgcatcc tgaaggatca
      361 gatcaaggtc aaggagggcg agatcgtcga tgccgcggtg atgtcgcgac gcgcactggc
      421 cacgttcatc gacgcgcaga tcgccagcgc gcgtggatcg ggcgttctgt tttcggtgca
      481 cttcaaggcc accatgatga aggtctcgga ccccatcatg ttcggtgtgg tggtggagga
      541 attcttccgc gacgtgctgg cgctgcacgg cgatgcgctg aagcaggccg gcttcaatcc
      601 gaacaacggg ttgggcgacc tctacgcctg cctcgcgaag ctcgacgacg accagcagca
      661 cgcgatcaag gccgacatcg aggcgcagta cgcccagcgt ccgcagctcg cgatggtcga
      721 ttccgacaag ggcatcacca acctgcacgt gccgtcggac gtgatcatcg acgcctcgat
      781 gccggcgatg atccgcgact cgggcaagat gtggaacgcc gacggtgagc gccaggactg
      841 caaggcggtg atccccgacc gcagctacgc cggcatctac gaggtgatga tcgaggactg
      901 caaggccaac ggcgcgttcg acccggccac gatgggcagc gtgccgaacg tgggcctcat
      961 ggccgacaag gccgaggaat acggctcgca cgacaagacc ttccagatcc ccgtcgatgg
     1021 ggcggtgcgg gtcgtcgacc aggatggcca ggcgatcttc gaacacgagg tcgaggccgg
     1081 tgacatctgg cgcatgtgcc agaccaagga cgcgcccgtc gcggactggg tgaagctggc
     1141 ggtgtcgcgc gcgcgcctgt cgaacacgcc ggcggtgttc tggctcgaca ggaatcgggc
     1201 gcacgatgcc gagatcatca agaaggtcga gcgttacctg gaggaccatg acacctcggg
     1261 cctgcacatc cagatcctcg cgcccgaaga cgcgatgcgg cattcgctcg agcgcatccg
     1321 cgacggcgag gacacgatct cggtcaccgg caacgtgttg cgcgactacc tcacggacct
     1381 gtttccgatc atggaactcg gcaccagcgc caagatgctg tcgatcgtgc cattgatggc
     1441 gggcggcggc ctgttcgaaa ccggcgcggg cggctcggcc cccaagcacg tccagcagtt
     1501 cgtggccgag gattacctgc gctgggattc gctgggcgag ttcctcgcgc tgggcgcctc
     1561 gctggaacat ctcgcccgca cgtacgacaa cgcgcgcgcc aaggtgctgg cggcggcgct
     1621 cgaccaggcc atcgggcgct tcctcgagac caacaagtcg cccgcgcgaa agatcggcga
     1681 gatcgacaac cgcggcagcc acttctatct cgcgatgtac tgggcgcgtg cgctggccga
     1741 ccagaaggat gacgcgggac tcgcgaagcc cttcggcaag ctcgccgacg aactcgaagc
     1801 tggcgaagcc tgcatcgtcg ccgaattgaa cggcgtacag ggcgagccgg tggacctcgg
     1861 cggttactac cgcccggaca tggcgaaggt gcgggcggcg atgcgcccca gcaagacctt
     1921 caacgacgcg ctggaagcct tgcgccgcga ggtgtgagcc gaaccgagga cggagcgggt
     1981 gccggttgcc gtccggcttc cgaaacgcgc gtccctcgca cgtgcggcag catgtcaagt
     2041 tagcttgaca tcgtaaaaac ctgccgttag agtcctctgt caagtacact tgacaaggcg
     2101 gggacgacgg ccatggccgc atggggaacc atcacgatgg gacgaatggc gatcggacgg
     2161 atggacgaat ggccggcctg gcagcgctgg ggcgcgctgg tgctggcgtg ggccctgtac
     2221 atcggcgggt tctggttgct gttcgccacc catgggctcg cggtgcgtgg actggcgctg
     2281 ctggtcctgt tggccgggtt gctgctgctc gtgtacgcct ccaaggcggt cctgaacgaa
     2341 cgcgtgcgca gcatcgatcg ctggcagatg cgggcggcgc tgccggcgtt cctggtctac
     2401 atcctgctgg cgctgtacgt cctgccgctg gtggaccaga tcaccacgcc ctggctgaaa
     2461 gcggccgcgg cgttgtcgcc gatggcgccg ctgctgttcg tggcgtgggt gatggtccgc
     2521 tacgtcaacc gttgcgacga gatggagcgg cgccagcaac tggaagccgc gggcgtcgcg
     2581 gtcctcgtgg tgggcctgct gggcatgtcg ctggggctgc tgtcggccgc gaagctggtc
     2641 gtgatcgacg gaacgttcgc gttgctgatg gtgctgccgg cgctggcggt ggtgtacggg
     2701 ctcgcctgcg tgctggcgaa gtggcggcat cgtgcgcgat gaagaatcgc gtccgggagt
     2761 tgcgtgaggc gcacggctgg tcgcagggtg cattggccga actcctggac gtgtcgcgcc
     2821 agaccatcaa cgcgatcgag aacggcaagt acgaccccag cctgccgctg gcgttcaagc
     2881 tggcgcggct gttcaagcaa cgcatcgaat ccatgttcga accggacgag ggacgctgaa
     2941 tgaaaatgaa cgcgctgaag ttgaatgcgc gcgtgctctt gcgcatcgcc gccatcgtga
     3001 tcgcggcggc ggtaatcggc tggcagtggt gggggaggga tcatgcgcgg gcgcccgcgg
     3061 cggacgaggc cggttcgctc gcggccaccg catcggcacc acccgtaccg ccggcggttg
     3121 cacccgcgtt cactgccccg cgaaaacccg cgactcccga aaagctggcg atgggcaagc
     3181 tcacgctgac ggcgtgccag ttgaaacagc cgcacagcgc ggtgaccacg cccgccttct
     3241 gtgcaaaatt tccggtgccg gagaatcgcg ccgaccccgg ctcgcgcacg atcgagctca
     3301 agctcgcggt catcaagagc gactcggcgg tgccggccag ggatctcgtc gtgtatctcg
     3361 ccggggggcc cgggcagtcc gccatcgaga cctatccgga aatggcgccg gcgttcgcgc
     3421 cgctgctgaa gcaccacgac ttgctgctgc tggaccagcg cggcaccggc ggctcgcacc
     3481 cgctgacttg ccccgacgtc gagaaggaac tggacaagtt cacggatgcg gcgccggacc
     3541 ccgcacgcat gaccgaaatg atcggcaagt gcgcggccga ggtcgagaag accgccgacc
     3601 cgcgctacta caccaccacc gacgccgtcg cggacctcga agccgtccgc caggcgctcg
     3661 gcgcgccgga gctggacctc gtcggcgttt cctacggcac gcgcatggcc cagcactacg
     3721 ccggcgcgca tcccgatgcc gtgcgcagca tcgtgctcga cggcgtggtg ccgaaccagc
     3781 tcgtgctcgg cgaatccttc gccgaagccc tggagcattc gctgaagctg cagtcggaag
     3841 cctgcaacgc ggcgcccgcg tgcaaaaaac ggttcgggaa ctggctcgcg accctgcacc
     3901 agttgcacgc gaagctcgag acggcgccgg cgcaacaggt cacattcgcc gatccctaca
     3961 gctacaagcc cgtcacccgc aagttgagcg cggacacgct cgccggcgtg gtgcgcttct
     4021 tctcctattc ggccgcgacc gcggcgctgc tgccgctggc ggtcgacgag gccgcgcacg
     4081 gcaattacgc gccgctgatg gggcaggcga aactcctgac cagcggcgtg tcgcagtcga
     4141 tgcagggcgg catgcagttc tcggtcgtct gcagcgagga cgcctcgctg ctggagcccc
     4201 gcccgcagga caaggacacc atcctcggct cctcgatgat cgacgacctg ctcgcggcct
     4261 gcaaggcatg gccgcatgcg gcgacgccga aggacttcca cgcgccgttc cggtcgtcga
     4321 tcccgacgct gctgatctcg ggcgaacgcg atccggtgac gccgccgtcc aacgccaacg
     4381 aagtgttgaa
//
LOCUS       DASSNH010000121        15269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_24365, whole genome shotgun sequence.
ACCESSION   DASSNH010000121 DASSNH010000000
VERSION     DASSNH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 15269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15269
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24365"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..405)
                     /gene="cyoD"
                     /locus_tag="VFK00_04310"
     CDS             complement(31..405)
                     /gene="cyoD"
                     /locus_tag="VFK00_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HET7592212.1"
                     /translation="MHEHQGSLRGYLTGFVLAAILTVIPFWLVMGDVIANKQLLILVI
                     LALAVVQIFVHIVYFLHLDTRSEAGWNMMAFIFTAVLVIIVLGASIWVMYNENMNMMP
                     MSPQYQQQHPISHQPTAEHPPQ"
     gene            complement(453..1079)
                     /gene="cyoC"
                     /locus_tag="VFK00_04315"
     CDS             complement(453..1079)
                     /gene="cyoC"
                     /locus_tag="VFK00_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HET7592213.1"
                     /translation="MNTTAVDTAPPAPAHFYALTESPPRNGTLLGFWIYLMSDCFIFA
                     SLFACYAVLGTEYAGGPTGAEVLDLPGLAVNTALLLVSSVVFGFAMLEMQRLRKGAML
                     GWLSIAGLLAAVFVALEVREFLVMIAEGAGPTRSAFLSSFFTLVATHGLHVTLGILWL
                     IVLMVQSIRFGLSEDNVRRMRCLSMFWHFLDLVWVGVFSYVYLVGVVR"
     gene            complement(1076..3064)
                     /gene="cyoB"
                     /locus_tag="VFK00_04320"
     CDS             complement(1076..3064)
                     /gene="cyoB"
                     /locus_tag="VFK00_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HET7592214.1"
                     /translation="MFGRLTLDSLPFLANNAEGRILLVTLAVVVIIGVVILGAITKYR
                     KWGYLWREWFTTVDHKKLGIMYMILGTVMLLRGFSDAIMMRAQQAYAFGPEMGYLPPQ
                     HYDQIFSAHGAIMIFFVGMAYIVGLMNYIMPLQIGSRDVAFPYLNNLSFWFTFGGAIL
                     FMVSLFIGDFSMAGWLGYPPVSELQQGPGVDYYIWGLQLSGVGTLLTGINFVVTILKM
                     RAPGMTLMKMPVFTWTALATSGLIIATFPVLTATLFLLGLDRYVGTHFFTDGFGGNLM
                     MYINLIWIWGHPEVYILILPLFGVFSEIVPTFSGKPLFAYSSMVYATIAIMILSYLVW
                     LHHFFTMGSGADVNSFFSLTTMMIAIPTGVKIFNWLFTMYRGRVRYTVPMLWAMGFLF
                     TFTIGGMTGVMLSVTPADFLLHNSLFLIAHFHNVIIGGVVFGVFAAVNFWFPKAFGFK
                     LDEFWGKASFWFWLVGFWVAFGPLYVLGLMGVTRRLSHFDDPSLQPYFIVAAFGAFLI
                     ALGILTFVIQIYVSILRRKQCTVGGDPWDGRTLEWSTSSPPPEYNFAFIPVVHHRDAW
                     ATMKNLGFRRPQGGYNDIHMPKNTATGVVIGALSFACAFGLTWYIWWLAALSLLGVIV
                     VSIAHSFNYHQHHHIPAEEVSRSESGSNHVTAAQGASA"
     gene            complement(3128..4120)
                     /gene="cyoA"
                     /locus_tag="VFK00_04325"
     CDS             complement(3128..4120)
                     /gene="cyoA"
                     /locus_tag="VFK00_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HET7592215.1"
                     /translation="MAMKRLRSVLLLPLLLLGGCNLVLLDPSGDVARQQSDIMIITTI
                     IIALIIVPVMIAIGVVAWRYRASNKDAKYDPHWDHSPQLELWVWAGPLLIIIAVGAVS
                     WIGTHQTDPYRAIDRIGSGKPVPADVKPLEVDVVSLRWKWLFLYPQYGIATVNELAAP
                     VDRPIKFKLTGDTMMDSFFIPALVGQVYTMPGMQTILHGVINKPGEYKGFSANYSGTG
                     FTDMRFTFHGLGTQDFDAWVGKVRAAGGDLDFPAYEKLREPSRAAPVQYYAKYAPTLY
                     DRILNMCVRPGEICRSEMMAADAHGGKAMQDMHAHDAMPAADAPKPAAPAADAH"
     gene            4328..5653
                     /locus_tag="VFK00_04330"
     CDS             4328..5653
                     /locus_tag="VFK00_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7592216.1"
                     /translation="MSASETLTHPAPGRPQSDSHDEHVTPGDLAVGVIVGRISQSFDV
                     FVFGLGCVLAFPEAFFPFASQLDGMFYAFAVFALGFVARPLGSMFFLVLQRHFGAATK
                     LTIALFMLGMATAGIAFMPGYVTLGWWSILALVLLRFGQGFGLGGSWNGLASLTALMA
                     PRERRGWYAMLAQLGEPIGFILAAGLFAYIWSSLTPEDFYAWGWRYPFFVAFAVNVVA
                     LFARLRMVVAPRYAEQMQHRDLEPSPIRETFRSQGVNIALGAFAPLASYALFYLVTIF
                     GLSWALLFTQMSTTGFLLLQIIGALVAIPCMLLSGRIADRFGRRTTLAVCGVLIAIYS
                     GWTALLLSGGMVNGYLFILVGFALLGFSHAQAAGAVNSGFPSEFRYTGAMITSDLGWL
                     IGAGFAPLVVLALAQFLGTDYVGLYLLSGAVGTLGALWLSARRGRLPND"
     gene            5999..11704
                     /locus_tag="VFK00_04335"
     CDS             5999..11704
                     /locus_tag="VFK00_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7592217.1"
                     /translation="MNARIKNRTLPESAAPSGDAEAPMHALLTAMQAVQRGDFSVRVS
                     GDWDGMEGRLAACFNDIIEANQRMARELERIGHGVGKQGKTRQRMSLAGRSGAWEDME
                     RSVNDLIDDLLWPTTEVTRAIAAVAKGDLTRTMALEFEGRPLEGAFLRTATIVNTMIE
                     QLSVFSSEVTRVAREVGTDGKLGGQAKVRGVSGVWRELTESVNQMASNLTAQVRNISE
                     VTIAVANGDLSRKITVDVRGEILQLKEAINTMVDQLRSFAAEVTRVAREVGTDGKLGG
                     QAVVPGVAGTWKDLTDSVNAMATNLTSQVRNIAEVTTAVARGDLSRKITVHVRGEILE
                     LKNTINTMVDQLNGFSAEVTRVAREVGTEGKLGGQAEVKGVSGVWKELTDNVNSMATN
                     LTNQVRNIAEVATAIARGDLSRKITVDVRGELLDLKETLNTMVEQLRSFAAEVTRVAR
                     EVGTEGNLGGQADVPGVAGTWKDLTDHVNSMASNLTAQVRNIAEVTTAVARGDLSRKI
                     TVDVRGEILELKNTINTMVDQLNSFAAEVTRVAREVGTEGELGGQADVPGVAGTWKDL
                     TDSVNAMATNLTSQVRNIAEVTIAVANGDLSRKITVDVRGEILQLKEAINTMVDQLNG
                     FAAEVTRVAREVGTEGKLGGQANVVGVSGVWEELTDNVNSMATNLTTQVRNIAEVTTA
                     VARGDLSRKIAVDVKGEILELKDTINTMVDQLNSFAAEVTRMAREVGTEGKLGGQAIV
                     PGVAGTWKDLTDNVNSMASNLTTQVRNIAEVATAVARGDLSRKIAVDAKGEILELKNT
                     INTMVDQLNSFAAEVTRMAREVGTEGKLGGQAIVPGVAGTWKDLTDNVNSMASNLTTQ
                     VRGIARVVTAVATGDLSKKLTVEAKGEIASLADTINSMTDTLAIFADQVTTVAREVGV
                     EGRLGGQAKVPGTAGIWKDLTANVNRLAANLTTQVRAIAEVATAVTQGDLTRSIQVDV
                     RGEVADLKNNINAMIGNLRATTERNREQDWLKTNLAKFSDMMQGQRDLITLGHMLLTE
                     LAPLVNAQQGLIYVVDSESGAPCLRRLAGYADANLASTEHRLAIGEGLIGQCAAQRQT
                     IVLKDLPNEAVQIRSGLIRTRPRSVIVLPVLFETEVKAVIELASLADFTETHRAFLEQ
                     LTGSVGIVLNTISATMRTERLLTQSQQLAGDLQAQQTELQQTNEELALKAKLLAEQNV
                     EVERKNQEIEHARRAVEEKAAELATTSRYKSEFLANMSHELRTPLNSILILGQQLADN
                     PDENLSDRQVEFARTIHAAGTDLLNLISDILDLSKIESGTVTVESEEIAFADLRGMIE
                     RSFTHEAERRKLAFSVEFDDALGRAINTDPKRLLQVINNLLSNAFKFTEKGSVRLRAR
                     VADEGWTGGHPILDKAPVVVAIAVEDTGIGISPDKQKIIFEAFQQADAGTARKYGGTG
                     LGLAISRELSALLGGEIRLHSQPGAGSTFTLYIPLNYMGPPVGYADEAKPAAALAVHP
                     LHSPEQVEDDRAVVAPGEPTLLIIEDDPRYAAVLRDLARGTGLRALVATSGADGLHLA
                     RQYQPTAVCLDIFLPDMLGWTVLARLKQTPETRHIPVQIVTVEAERHHGMERGAFSYL
                     TKPATTDAIEASLDRITQFALRPVKKLLVVEDDNTARDSIVTLIDHEDVEITSAGTGK
                     DALEKIHSGVFDCVVLDLRLPDMSGFELLDRLKADAGTREVPIVVFTGKDLSPEEEQR
                     LLKVARSIIVKGVHSPERLLDETALFLHRVVSKLPPAKREMLETLYESDSSLVNKKVL
                     VVDDDVRNIFAISSILERRDMQVLTAENGRDAVALVEAQEDIAMVLMDIMMPGMDGYE
                     TIRAIRARPELRSLPIIALTAKAMKGDREKCIEAGASDYIAKPVHTDQLLSLLRQWLH
                     R"
     gene            11704..13446
                     /locus_tag="VFK00_04340"
     CDS             11704..13446
                     /locus_tag="VFK00_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592218.1"
                     /translation="MNDAFAHGEPANVLLVDDNPARLLSYRTILEPLGERLIEAASGR
                     EALRTVMDTEFAVIVLDVNMPGMDGFETASLIHQHPRFEKTPIIFASAINVSDMDRLQ
                     GYKLGAVDYVMVPIIPEILRGKVAVLAELFRKRRELQRVNGELADTNARLARSNEALR
                     IEREREVHKLNEALTDSNVKLATSNRELKQEVDERRRIEERLREADRRKDEFLATLAH
                     ELRNPLAPIQSALNVYRLGHDVGRADDGTLYQIFERQTGLLVHMVDDLLDVSRITRGR
                     LQLRLERALLADILTAAVETAQPLIDEGQQRLELDLPSQPVHLNADPHRLTQVFGNLL
                     NNACKFSDPGGAITVRAKLGEGTVAIEIHDTGIGLTEQQVRFIFDLFAQADTSLERPH
                     SGLGIGLTLSQRLIELHGGEIHASSEGLGSGSTFTVTLPVAVAGPASAGGSAPAAEEA
                     AVGSLRIMVVDDNRDSADMLAMSLRIMGHQARAVYSPLAVMDACDEFEPELMIMDIGM
                     PELNGLVLAERLRAHAWRGRAPRLVALTGWGQAEDRKRSLAAGFADHLVKPINLATIE
                     RVCRDTWAAACEAT"
     gene            complement(13550..14341)
                     /locus_tag="VFK00_04345"
     CDS             complement(13550..14341)
                     /locus_tag="VFK00_04345"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET7592219.1"
                     /translation="MATVPRPVFATPKLAFVASTSDNARAALDELQGRYGTVAPEDAD
                     VIVALGGDGFMLRTLHAELDRGLPVYGMKFGRVGFLMNRYLPDELPARIAAARPAVLH
                     PLKMHVTQRDGNEVEALAFNEISLLRQTNQAAHIEVALNGHVKLANLICDGLLVATPA
                     GSTAYNLSAHGPILPLDANVLALTPISPFRPRRWRGALLPFATEVGLRTLEPDHRPVS
                     ATADFHEVRGAVKIRVHQARKRSITLLFDPERSLEQRILDEQFEL"
     gene            complement(14553..>15269)
                     /locus_tag="VFK00_04350"
     CDS             complement(14553..>15269)
                     /locus_tag="VFK00_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7592220.1"
                     /translation="AALEQDGFVLYYQPIVSLQGDEGEFFEVLVRMQGPTEEVLPAHF
                     LPIAERNDRMSKIDRWVIGKAIDALAKTLGKPHPSTLFVKLSTLTLHDETLADWVDEK
                     LASAGVKASQLVFEVPEAKVMTCLKQAHDFVERWRKSGGAFELEQFGSGLNSFQSLKH
                     IDADYLKLDRALTSDLAHRSENELKVRELCSQAHAAGKRTVVEWIEEASSMPALFSAG
                     VDFAQGNFLASPVPEMRYVT"
BASE COUNT         2808 a         4916 c         5123 g         2422 t
ORIGIN      
        1 ctacaacagc gcccaacagt ccccggggtt ttactgcggc gggtgctcgg cggtcggctg
       61 gtggctgatc gggtgctgct gctggtactg cggcgacatc ggcatcatgt tcatgttctc
      121 gttgtacatg acccagatgg atgcgccgag cacgatgatc accagcaccg cggtgaagat
      181 gaaggccatc atgttccagc cggcttccga gcgcgtgtcg aggtgcagga aatacacgat
      241 gtgcacgaag atctgcacga ccgccagcgc caggatgacg aggatcagca attgcttgtt
      301 ggcgatcacg tcgcccatca cgagccagaa cgggatcacc gtcaggatcg cggccagcac
      361 gaacccggtc aggtagccgc gcaggctgcc ctggtgttcg tgcatcggat cgcggccgtc
      421 gtgcggcacc gcgtgaccgg gcgaaggctg gttcatcgca ccactcccac cagatagacg
      481 tagctgaaga ccccgaccca gaccaggtcg aggaaatgcc agaacatgct gaggcaacgc
      541 atccggcgca cgttgtcctc gctgaggccg aagcggatcg actgcaccat cagcacgatg
      601 agccacagga tgccgagcgt cacgtgcagg ccgtgcgtcg ccaccagcgt gaagaaggac
      661 gacaggaacg cgctgcgggt cgggccggcg ccctcggcga tcatcaccag gaactcgcgg
      721 acttccagcg cgacgaacac cgccgcgagc aacccggcga tcgacagcca gcccagcatc
      781 gcgcccttcc tcaggcgctg catctccagc atcgcgaagc cgaacaccac cgacgatacc
      841 agcagcagcg cggtgttcac cgcgagtccg ggcaggtcca gcacttccgc gccggtggga
      901 ccgcctgcgt actcggtgcc gagcaccgcg tagcaggcga acagcgacgc gaagatgaag
      961 cagtcgctca tcaggtagat ccagaacccg agcagggtcc cgttgcgcgg cggcgactcc
     1021 gtcagggcgt agaagtgcgc gggcgccgga ggtgcggtgt cgacggcggt ggtattcatg
     1081 cgctggcgcc ttgtgctgcc gtcacgtggt tgctgccgga ctcggaacgg ctcacttctt
     1141 cggcgggaat gtgatggtgc tggtggtaat tgaagctgtg ggcgatgctc acgacgatca
     1201 cgcccagcag cgacagcgcc gccagccacc agatgtacca ggtcagcccg aacgcacagg
     1261 cgaacgacag cgcgccgatc accacgcccg tcgcggtgtt cttcggcatg tggatgtcgt
     1321 tgtagccgcc ttgcggacgc ctgaagccga ggttcttcat ggtcgcccac gcgtcgcggt
     1381 ggtgcacgac cgggatgaac gcgaagttgt attcgggtgg cggtgaactg gtggaccact
     1441 cgagcgtgcg gccgtcccac ggatcgccgc ccaccgtgca ttgcttgcgc cgcaggatgc
     1501 tgacgtaaat ctggatcacg aaggtgagga tgccgagcgc gatcaggaac gcgccgaacg
     1561 cggcgacgat gaaatacggc tgcaaactcg gatcatcgaa gtggctcaaa cgccgcgtca
     1621 cacccatcaa accgagcacg tacagcgggc cgaatgcgac ccagaaaccc accagccaga
     1681 accagaacga cgccttgccc cagaactcgt cgagcttgaa cccgaacgcc ttggggaacc
     1741 agaagttcac ggccgcgaac acgccgaaca ccaccccgcc gatgatcacg ttgtggaagt
     1801 gggcgatcag gaacaggctg ttgtgcagca ggaagtcggc cggcgtcacc gacagcatca
     1861 cgccggtcat gccgccgatg gtgaaggtga acaggaaacc catcgcccac agcatcggca
     1921 cggtgtagcg cacgcggccg cggtacatcg tgaacagcca gttgaagatc ttcaccccgg
     1981 tcgggatcgc aatcatcatc gtggtcagcg aaaagaacga gttgacgtcg gcgcccgaac
     2041 ccatcgtgaa gaagtggtgc agccacacca ggtacgacag gatcatgatc gcgatggtgg
     2101 cgtacaccat cgaagagtag gcgaacagtg gtttgcccga gaacgtcggc acgatctccg
     2161 agaacacgcc gaacagcggc aatatcaata tgtagacctc ggggtggccc cagatccaga
     2221 tcaggttgat gtacatcatc aggttgccgc cgaagccgtc ggtgaagaag tgcgtgccga
     2281 cgtagcggtc gaggccgagc aggaacagcg tcgcggtgag caccgggaag gtcgcaatga
     2341 tcagcccgct cgtggcgagc gccgtccatg tgaacaccgg catcttcatc agcgtcatgc
     2401 cgggcgcgcg catcttgagg atcgtcacca cgaagttgat gccggtgagc agggtgccga
     2461 cgcccgacaa ctgcaggccc cagatgtagt agtcgacgcc ggggccttgc tgcagttccg
     2521 acacgggggg ataacccagc cagccggcca tcgagaagtc gccaatgaac agcgacacca
     2581 tgaacaggat ggcgccgcca aaggtgaacc agaaactcaa gttgttgagg tacggaaacg
     2641 cgacgtcacg cgagcctatc tgcagcggca tgatgtaatt catcaggccg acgatgtagg
     2701 ccatgccgac aaagaagatc atgatcgcgc cgtgcgcgga gaagatctgg tcgtagtgct
     2761 gtggcggcag gtaacccatt tccgggccga aggcgtaggc ctgctgcgcg cgcatcatga
     2821 tcgcgtcgga aaaaccgcgc agcagcatca cggtaccgag gatcatgtac atgatgccga
     2881 gcttcttgtg gtccacggtg gtgaaccatt cgcgccacag gtaaccccat ttgcggtatt
     2941 tggtgatggc gcccaggatc accacgccga tgatcaccac caccgcgagc gtcaccagca
     3001 ggatgcgacc ctcggcgttg ttggcgagga agggcagcga atcgagggtc agccgtccga
     3061 acaggaagtt ccagatggcg gcgcggttgg gcatggcttc aagcatgatc gaatcccgtg
     3121 aaggcagtca gtgcgcgtcc gcggcaggcg cagccggctt cggcgcatcg gccgcgggca
     3181 tggcgtcgtg cgcgtgcatg tcctgcatgg ctttgccgcc gtgcgcatcg gcggccatca
     3241 tctcgctcct gcagatttcg ccgggacgca cgcacatgtt caggatgcgg tcgtacaggg
     3301 tcggtgcgta tttcgcgtag tactgcaccg gcgcggcgcg gctgggttcg cgcagcttct
     3361 cgtaggccgg gaagtcgaga tcgccgcccg ccgcgcgcac cttgcctacc caagcgtcga
     3421 aatcctgcgt gcccaggcca tggaacgtga agcgcatgtc ggtgaagccc gtgccgctgt
     3481 agttggcgga aaagcccttg tactcgccgg gcttgttgat gacgccgtgc aggatcgtct
     3541 gcatgccggg catcgtgtag acctgaccca ccagcgccgg gatgaagaac gaatccatca
     3601 tggtgtcgcc ggtcagtttg aacttgatcg gacgatcgac cggcgcggcc aattcattca
     3661 cggtcgcgat gccgtactgc ggatacagga acaaccattt ccagcgcagc gacaccacgt
     3721 ccacttccag cggcttgaca tccgccggca ccggtttgcc cgagccgatg cggtcgatcg
     3781 cgcgatacgg atcggtctgg tgggtgccga tccagctcac cgcacccacc gcgatgatga
     3841 tcagcagcgg ccccgcccac acccacagtt cgagctgcgg cgaatgatcc cagtgcggat
     3901 cgtacttggc gtccttgttg gaagcgcggt agcgccacgc gacgacgccg atcgcaatca
     3961 tcaccggcac gatgatcagc gcgatgatga tcgtggtgat gatcatgatg tcgctctgct
     4021 ggcgcgcgac gtcacccgat ggatccagca gcaccaggtt gcagccgccg agcagcaaga
     4081 ggggaagcag caacacgctg cgaaggcgct tcattgccat ggacgcactt gggtcgtatg
     4141 cagaaagcgc ctatgatacc ccttcactcc atctttgcag ccttggcggc gatgcgaaca
     4201 agccatcacg ccgttttgat cggcatcaat gcggtgtgtc gaagggacgc ttgaatgccg
     4261 ttcgccgcgt ccggcgcacg ccgcgtcaca catccaacat ccgctgcact caagccaggc
     4321 aaacgtcatg tccgccagcg aaaccctgac ccacccggcg cccggccgtc cacaatcgga
     4381 ttcacacgac gaacacgtca cgccgggcga cctggcggtc ggcgtgatcg tcggacgcat
     4441 ttcccagtcg ttcgatgtgt tcgtgttcgg gttgggttgc gtgctggcgt tccccgaggc
     4501 atttttcccg ttcgccagcc agttggacgg gatgttctac gccttcgcgg ttttcgcgct
     4561 ggggttcgtc gcgcggccgc tgggctcgat gttcttcctc gtgctgcagc gccacttcgg
     4621 tgccgcgacc aagctgacga tcgcgctttt catgctcggc atggcgaccg ccggcatcgc
     4681 gttcatgccg ggctacgtga cgctcggctg gtggtcgatc ctggcgctgg tcctgttgcg
     4741 cttcgggcag ggcttcgggc tgggcggaag ctggaatggc ctggcctcgc tgaccgcgct
     4801 gatggccccg cgcgagcgcc gtggctggta tgccatgctc gcccagctcg gcgagcccat
     4861 cggcttcatc ctggccgccg gcctgttcgc ctacatctgg tccagcctga cgccggaaga
     4921 cttttacgcc tggggctggc gttacccgtt cttcgtggcc ttcgcggtca acgtggtggc
     4981 gttgttcgcg cgcctgcgca tggtggtcgc gccgcgttac gccgagcaga tgcagcatcg
     5041 cgatctggaa ccctcgccga tccgcgagac gttccgctcg caaggcgtca acatcgcgct
     5101 gggcgcgttc gcgccgctcg cgagttacgc gttgttctac ctggtgacga tcttcggctt
     5161 gtcgtgggcg ctgctgttca cgcagatgtc gacgaccggg ttcctgctgc tgcagatcat
     5221 cggcgcgctg gtggccattc cgtgcatgtt gctgtcgggc cgcatcgccg atcgcttcgg
     5281 ccgtcgcacc acgttggccg tgtgcggcgt gttgatcgcg atctacagcg gctggaccgc
     5341 gctgctgctg tcgggtggca tggtgaacgg ctacctgttc atcctcgtcg gtttcgcgct
     5401 gctcggcttc tctcacgcac aggccgcagg cgcggtcaat tccggattcc cgagcgagtt
     5461 ccgttacacc ggtgcgatga tcacgtccga ccttggctgg ctgatcggtg caggcttcgc
     5521 gccgctggtg gtattggcgc tggcgcagtt cctcggcacg gactacgtgg gcctgtacct
     5581 gctctcgggt gccgtgggca cgctcggagc gctgtggctg tcggcgcggc gcggccgctt
     5641 gccgaacgac tgaagcagag gcggcagggt cgggcccctg cgagggcagg cccggggccg
     5701 gtttgcgcgc aaaccgtgcc gggcacccca tttgccacgc cgttatccgc tatgctctcg
     5761 gcgctggccg gtcgtcgagc gcatcggatt gcttggcagg cactgacccg acccagcgcc
     5821 accggtcctg aaaccaggag aatcagagca tgatagggat ttccgtgcac tgctccgcag
     5881 cgggcgcgat tgcgggaaaa accgggcgag cgttccgttc gatgcctcgt ccccatggcg
     5941 cgactgcgct cgtgccggcc cgaactgctt gcgcggccat ctccctccgg agatcaccat
     6001 gaatgccagg atcaagaacc ggacccttcc ggaatcggca gcgccgtcgg gcgacgccga
     6061 agcgcccatg catgcgttgc tgacggccat gcaggcggtg cagcgcggtg atttcagcgt
     6121 gcgggtcagc ggcgactggg acggcatgga aggcaggctc gccgcctgct tcaacgacat
     6181 catcgaagcc aaccagcgca tggcgcgcga actggagcgc atcggccacg gcgtcggcaa
     6241 gcagggcaag acccgccagc gcatgtcgct ggccggccgc agcggcgcct gggaggacat
     6301 ggagcgctcg gtcaacgacc tgatcgacga cctgttgtgg ccgaccaccg aggtcacgcg
     6361 cgcaatcgcg gcggtggcca agggcgatct cacccgcacc atggcgctgg aattcgaggg
     6421 ccggccgctg gagggcgcgt tcctgcgcac ggcgacgatc gtgaacacca tgatcgagca
     6481 gctcagcgtg ttcagttccg aagtcacgcg cgtggcgcgc gaggtcggta ccgacggcaa
     6541 gctcggcggc caggccaagg tgcgcggcgt cagcggcgtg tggcgcgagc tgaccgaatc
     6601 ggtcaaccag atggcctcga acctgaccgc gcaggtgcgc aacatctccg aggtcacgat
     6661 cgcggtggcc aacggcgacc tgtcacgcaa gatcacggtc gacgtgcgcg gcgagatcct
     6721 gcaattgaag gaagccatca acacgatggt ggaccagttg cgctcgttcg cggccgaggt
     6781 gacccgcgtg gcccgcgagg tcggcaccga cggcaagctc ggcggccagg cggtggtgcc
     6841 gggcgtggcc ggaacctgga aagacctgac cgattcggtc aacgccatgg ccaccaacct
     6901 tacgtcccag gtgcgcaata tcgccgaggt cacgaccgcc gtggcgcgcg gcgatctgtc
     6961 gcgcaagatc acggtgcacg tgcgcggcga aatcctggag ctcaagaaca ccatcaacac
     7021 gatggtggac cagttgaacg gcttctcggc cgaggtgacg cgcgtggcgc gcgaggtcgg
     7081 caccgagggc aagctgggcg gccaggccga ggtcaagggc gtcagcggcg tgtggaagga
     7141 gctgaccgac aacgtcaact cgatggcaac caacctgacc aaccaggtac gcaacatcgc
     7201 ggaggtggcg accgcgattg cgcgcggcga cctgtcgcgc aagatcacgg tggacgtgcg
     7261 cggcgagctg ctcgacctca aggaaacgct gaacacgatg gtcgagcagt tgcgctcgtt
     7321 cgcggccgag gtcacgcgcg tggcgcgcga ggtcggcacc gaaggcaacc tgggtgggca
     7381 ggccgacgtg cccggcgtcg cgggcacgtg gaaggacctc accgatcacg tgaactcgat
     7441 ggcgtcgaac ctgaccgcgc aggtccgcaa catcgccgag gtcacgacgg cggtggcgcg
     7501 cggcgacctg tcgcgcaaga tcacggtgga cgtgcgcggc gaaatcctgg agctgaagaa
     7561 caccatcaac accatggtcg accagttgaa cagcttcgcc gccgaggtga cgcgcgttgc
     7621 gcgcgaggtc ggcaccgagg gcgaactcgg cggccaagcc gacgtgcccg gcgttgcggg
     7681 gacctggaag gacctgaccg actcggtcaa tgcgatggcc accaacctga cttcgcaagt
     7741 gcgcaacatc gccgaggtca ccatcgcggt ggcgaacggc gatctgtcgc gcaagatcac
     7801 ggtagacgtg cgtggtgaaa tcctgcagct caaggaagcc atcaacacca tggtcgacca
     7861 gttgaacggc ttcgccgccg aggtgacgcg cgtggcgcgc gaggtcggca ccgagggcaa
     7921 gctgggtggc caggcaaatg tggtcggtgt cagcggcgtg tgggaggaac tgaccgacaa
     7981 cgtcaattcg atggcgacca acctcacgac ccaggtccgc aacatcgccg aggtcaccac
     8041 cgcggtcgcg cgcggcgacc tgtcgcgcaa gatcgcggtc gacgtcaagg gcgagatcct
     8101 ggagttgaag gacaccatca acacgatggt ggaccagttg aacagcttcg ccgccgaagt
     8161 gacgcgcatg gcgcgcgaag tcggcaccga gggcaagctg ggcgggcagg cgatcgtgcc
     8221 gggcgtcgcg ggtacgtgga aggacctgac cgacaacgtc aactcgatgg catcgaactt
     8281 gacgacccag gtgcgcaaca tcgccgaggt cgcgacggcc gtcgcgcgcg gcgacctgtc
     8341 gcgcaagatc gcggtcgatg ccaagggcga gatcctggag ctcaagaaca ccatcaacac
     8401 gatggtggac cagttgaaca gcttcgccgc cgaggtgacg cgcatggcgc gcgaagtcgg
     8461 caccgagggc aagctgggcg ggcaggcgat cgtgccgggc gtcgcgggca cctggaagga
     8521 cctgaccgac aacgtcaact cgatggcgtc gaacctcacc acccaggtgc gaggcatcgc
     8581 gcgggtggtg acggcggtcg ccaccggcga cctcagcaag aagctgaccg tggaggccaa
     8641 gggcgagatc gcctcgctgg ccgacaccat caacagcatg accgacacgc tcgccatctt
     8701 cgccgaccag gtgaccacgg tggcgcgcga ggtcggcgtc gaagggcgcc tcggcggcca
     8761 ggccaaggtg ccgggcaccg ccggcatctg gaaggacctg accgccaacg tgaaccggct
     8821 ggcggcaaac ctcaccaccc aggtgcgcgc gatcgccgag gtcgcgaccg ccgtgacgca
     8881 gggcgacctg acccgttcga tccaggtcga cgtgcgcggc gaagtcgccg acctcaagaa
     8941 caacatcaac gcgatgatcg gcaacctgcg cgccaccacc gagcgcaatc gcgagcagga
     9001 ctggctgaag accaatctcg cgaagttcag cgacatgatg cagggccagc gcgacctgat
     9061 cacgctgggc cacatgctgc tgaccgaact ggcgcccctg gtgaacgcgc agcagggcct
     9121 gatctacgtg gtcgacagcg aatcgggcgc gccgtgcctg cggcggctgg caggctacgc
     9181 cgacgccaac ctggcgagta cggaacaccg cctcgcgatc ggcgaaggcc tgatcgggca
     9241 gtgtgcggcg cagcgccaga cgatcgtgct gaaggacctg ccgaacgagg cggtgcagat
     9301 ccgatcggga ttgatcagga cccgtccgcg cagcgtcatc gtgctgccgg tgctgttcga
     9361 aaccgaggtc aaggcggtga tcgaactggc ctcgctggcc gacttcaccg agacccaccg
     9421 cgccttcctc gagcagctca cgggcagcgt cgggatcgtg ctcaacacca tctccgcgac
     9481 catgcgcacg gagcgcttgc tgacccagtc gcagcaactg gcgggcgacc tgcaggcgca
     9541 gcagacggaa ctgcagcaga ccaacgagga actcgcgttg aaggccaagc tgctggccga
     9601 gcagaacgtc gaggtcgagc gcaagaacca ggaaatcgag cacgcccgcc gcgccgtcga
     9661 ggaaaaggcg gccgagctcg cgacgacctc gcgctacaag tccgagttcc tcgccaacat
     9721 gtcgcacgag ctgcgcacgc cgctcaacag catcctgatc ctcgggcagc aattggccga
     9781 caacccggac gagaacctga gcgatcgcca ggtggaattc gcgcggacca tccacgccgc
     9841 cggcaccgac ctgctgaacc tcatcagcga catcctggac ctgtccaaga tcgagtccgg
     9901 tacggtcacc gtcgaaagcg aggagatcgc gttcgccgac ctgcgcggca tgatcgagcg
     9961 cagcttcacc cacgaagccg agcgccgcaa gctcgctttc tcggtggagt tcgacgatgc
    10021 cttggggcgc gcgatcaaca ccgatcccaa gcgcctgctt caggtcatca acaacctgct
    10081 ctcgaacgcc ttcaagttca ccgagaaggg cagcgtgcgc ctgcgcgcgc gggtggccga
    10141 cgagggctgg acgggcggcc atccgatcct agacaaggcg ccggtagtgg tggcgatcgc
    10201 ggtcgaggac accggcatcg gcatctcgcc cgacaagcag aagatcatct tcgaagcctt
    10261 ccagcaggcc gatgcaggca ccgcgcgcaa gtacggcggc acgggactcg gcctcgcgat
    10321 cagccgcgag ctgtcggccc tgctcggcgg cgaaatccgg ctgcacagcc aacccggcgc
    10381 cggcagcacc ttcactttgt acatcccgct caattacatg ggtccgccgg tcggctacgc
    10441 ggacgaggcc aagcctgccg cggccctggc cgtccatccg ctgcattcgc ccgagcaggt
    10501 cgaagacgat cgcgcggtcg tcgcgccggg cgagccgacc ttgctcatca tcgaggacga
    10561 cccccgctat gccgccgtgc tgcgggacct tgcgcggggc accggactgc gcgcgctcgt
    10621 cgcgacatcg ggcgccgacg ggctgcacct cgcgcggcaa taccagccca ccgcggtctg
    10681 cctcgacatc ttcctgcccg acatgctcgg ctggacggtg ttggcgcggc tgaaacagac
    10741 gccggagacc cgccacatcc cggtgcagat cgtcacggtc gaagccgagc gccaccatgg
    10801 catggagcgc ggcgcatttt cgtatctcac caagccggcc accaccgatg cgatcgaagc
    10861 gtcgctcgac cgcatcacgc agttcgcgct gcgtccggtc aagaagttgc tggtggtcga
    10921 ggacgacaac accgcgcgcg acagcatcgt cacgctgatc gaccacgagg acgtcgaaat
    10981 cacgtccgcc ggaaccggca aggacgcgct cgagaagatc cactccggtg tgttcgactg
    11041 cgtggtcctg gatctgcggt tgccggacat gagcggcttc gaactgctcg atcggctcaa
    11101 ggccgacgcc ggtacccggg aggtgccgat cgtggtcttc acgggcaagg acctctcgcc
    11161 ggaggaagag cagcgcctgc tgaaggtcgc gcgcagcatc atcgtcaagg gcgtgcattc
    11221 gcccgagcgg ctgctcgacg aaaccgcgct gttcctgcac cgcgtcgtgt cgaagctgcc
    11281 gccggccaag cgcgagatgc tcgagaccct ctacgagtcg gacagctcgc tcgtcaacaa
    11341 gaaggtgctg gtggtcgacg acgacgttcg caacatcttc gccatcagca gtatccttga
    11401 gcgccgcgac atgcaggtgc tgaccgcgga gaacggccgg gatgccgttg cgttggtgga
    11461 agcccaggaa gacatcgcca tggtactgat ggacatcatg atgccgggca tggatggtta
    11521 cgaaaccatc cgcgcgatac gcgcccggcc cgagctgcgt tcgctgccga tcatcgcgct
    11581 gaccgcgaag gcgatgaagg gcgaccgcga gaaatgcatc gaggccggtg cgtcggacta
    11641 catcgccaag ccggtgcaca ccgaccagtt gctgtccctg ctgcgccaat ggttgcaccg
    11701 ctgatgaacg acgcgtttgc ccacggcgag cccgccaacg tcctgctggt cgacgacaat
    11761 cccgcgcgcc tgctgagcta ccgcacgatc ctggagccgc tcggcgagcg gctgatcgag
    11821 gccgcctcgg gcagggaggc gttgcggacc gtgatggaca ccgagttcgc ggtgatcgtg
    11881 ctggacgtga acatgcccgg catggatggt ttcgaaaccg ccagcctgat ccaccagcat
    11941 ccgcgcttcg agaagacgcc gatcatcttc gcgtcggcga tcaacgtcag cgacatggac
    12001 aggctgcagg gctacaagct cggcgcggtc gactacgtca tggtgccgat catccccgaa
    12061 atcctgcggg gcaaggtcgc ggtgctggcc gaactgttcc gcaagcggcg tgagctgcaa
    12121 cgggtcaacg gcgaactggc ggacaccaat gcgcgcctcg cgcgcagcaa cgaggcgctg
    12181 cgcatcgagc gcgagcgcga ggtgcacaag ctcaacgagg cgctcaccga cagcaacgtg
    12241 aagctggcca cgagcaatcg cgagctcaag caggaagtgg acgagcgccg ccgcatcgag
    12301 gagcgcttgc gcgaggcgga ccgccgcaag gacgagttcc tggcgacgct ggcgcacgaa
    12361 ttgcgcaacc cgctcgcgcc gatccagagc gcgctcaacg tctaccggct cgggcacgac
    12421 gtcggccgcg cggacgacgg cacgctctac cagatcttcg agcgccagac cggcttgctc
    12481 gtgcacatgg tcgacgacct gctggacgtt tcgcgcatca cccgtggccg gctgcaattg
    12541 cggctcgaac gtgcgctgct cgccgacatc ctgaccgccg cggtcgaaac cgcgcagccg
    12601 ctgatcgacg aaggccagca gcgcctggag ctggacctgc cctcgcagcc ggtgcacctc
    12661 aacgccgacc cccaccgact gacccaggtg ttcgggaacc tgctcaacaa cgcctgcaag
    12721 ttttccgatc ccggcggcgc catcaccgtg cgcgcgaagc tcggcgaagg aaccgtcgcc
    12781 atcgagatcc acgacaccgg catcgggctg accgagcagc aggtgcgttt catcttcgac
    12841 ctgttcgcgc aggcggacac ctcgctggaa cgcccgcaca gcggcctcgg catcgggctg
    12901 acgttgtcgc aacggctgat cgagttgcac ggcggcgaga tccacgccag cagcgaaggc
    12961 ctcggcagcg gcagcacctt cacggtgacg ttgccggtcg cggttgccgg cccggcttcg
    13021 gcggggggat ccgcgccagc ggcggaagag gcggcagtgg gcagcctgcg gatcatggtg
    13081 gtggacgaca accgcgattc cgccgacatg ctggcgatgt cgttgcggat catgggccac
    13141 caggctcgcg ccgtgtactc cccgctggcg gtgatggacg cctgcgacga gttcgaaccc
    13201 gagctgatga tcatggacat tggcatgccc gagctcaacg gcctggtctt ggccgagcgg
    13261 ttgcgcgcgc acgcgtggcg cggccgggcg cccaggctgg tggccctgac aggctggggc
    13321 caggccgagg accgcaagcg ttcactggcg gccggctttg cggatcacct ggtcaagccg
    13381 atcaacctcg ccacgatcga gcgggtctgc cgcgacacct gggccgccgc ctgcgaggcg
    13441 acctagggga ggttgggccg agcaacacgc agcccaggtc aaagtattgg cgttggcttt
    13501 cccccaggaa gcgcgggtca gctcaacctg cgaatggcac gctggtggct cacagctcga
    13561 actgttcgtc gaggatgcgt tgctcgaggc tgcgctcggg gtcgaagagc agggtgatgg
    13621 agcgtttgcg cgcctggtgc acgcggatct tcacggcgcc gcggacttcg tggaagtcgg
    13681 cggtggcgct gaccgggcgg tggtctggct cgagcgtgcg caagccgacc tcggtcgcga
    13741 acgggagcag ggcgccgcgc cagcggcgcg ggcggaaggg gctgatcggg gtcagcgcca
    13801 gcacgttggc atccagcggc aggatcggac cgtgcgcgga caggttgtac gcggtggaac
    13861 cggcgggcgt cgcgaccagc aggccgtcgc agatcagatt cgcaagtttc acgtggccgt
    13921 tcagtgccac ttcgatgtgg gcggcctggt tggtctggcg cagcagcgag atttcgttga
    13981 acgccagcgc ctcgacttcg ttgccgtcgc gctgcgtgac gtgcatcttc aggggatgca
    14041 gcaccgcggg gcgtgcggcc gcgatccgcg cgggaagttc gtcggggaga tagcggttca
    14101 tcaggaaacc gacgcggccg aacttcatgc cgtacaccgg caggccgcgg tccagttcgg
    14161 cgtgcagcgt gcgcagcatg aagccgtcgc cgcccagcgc cacgatgacg tcggcgtcct
    14221 cgggcgccac cgtgccgtag cggccctgca gttcgtcgag ggctgcgcgc gcgttgtccg
    14281 acgtggacgc gacgaatgcc agtttcgggg tggcgaaaac cgggcgcgga acggtggcca
    14341 tgggtacagt gtgccgtgaa aggggggcca gggaaacttg tgttgtcgtc gccggacatc
    14401 ccccggcgct aggcgcccgc gctccgcgcg gccgctacgc gcccgcgctt cgcgcgcgtt
    14461 cgcttcgctc cgcccttcgt tccgaagggg tgacagtgtt gcaagtcgcc gcgctgtacc
    14521 tagttccgag cccgagtccc gagtcccaag cctcaggtta cgtaccgcat ttccggcacg
    14581 ggggacgcga ggaagttgcc ttgcgcgaaa tccacgcccg ccgagaacag ggccggcatg
    14641 ctggaagctt cctcgatcca ttcgacgacg gtgcgcttgc cggcggcgtg ggcctgcgag
    14701 cacaactcgc gcaccttcag ttcgttttcg gaacggtggg cgaggtccga ggtcagcgcg
    14761 cgatcgagct tcaggtagtc ggcgtcgatg tgcttcagcg actggaacga attgaggccg
    14821 gagccgaatt gctccagttc gaaggcgccg ccgctcttgc gccagcgctc gacgaaatcg
    14881 tgcgcctgct tgaggcaggt catcaccttg gcttccggaa cctcgaacac gagctgcgaa
    14941 gccttgacac ctgccgaggc gagcttctcg tccacccagt cggccagcgt ttcgtcgtgc
    15001 agggtgagcg tggagagctt gacgaacagc gtggagggat gtggtttccc gagggttttc
    15061 gccagcgcgt cgatcgcctt gccgatgacc cagcgatcga tcttcgacat gcggtcgttg
    15121 cgttccgcga tcggcaggaa gtgcgcgggg aggacttcct cggtcgggcc ctgcatgcgc
    15181 accagcacct cgaagaattc gccttcgtcg ccctgcaggc tgacgatcgg ctggtagtag
    15241 aggacgaagc cgtcctgctc gagggcggc
//
LOCUS       DASSNH010000122         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_951305, whole genome shotgun sequence.
ACCESSION   DASSNH010000122 DASSNH010000000
VERSION     DASSNH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951305"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VFK00_04355"
     CDS             complement(<1..1652)
                     /locus_tag="VFK00_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592221.1"
                     /translation="MAGESVEDRLAAVERRLAQLEERLAARFVPKVPPEPPQAQGGQP
                     QPGEIWLRDVPPHAQPAVAAPATPAAPEPAHVAPAQAARVPAPPRGSPVTSILGWGGA
                     LAFLLAAAYVIRLGIDSGWLTPVVQVSSVVAGGIALIGAGFGLRGSWRRYAGYLPACG
                     VVMLFLAIYGAHLHYELIGVKAAGLFVVAVCALSLWLCRAFDSDLYALFAVAGSYSAP
                     FLLASSTGSITDLVVYYSAWGVVFSVYAIWHGRRLIYLLAAYLALVGFDGAWHVHASG
                     QWVAALVFQAAQFVIFGVATAWFSVRRREPMDTATAALHLPALLLFYALQYYVLDRHL
                     PALAPWISFASLAAVGLLYWGARGYLRRPLPGGEFLLWCYLSLVLFHAGYLEALPKQM
                     APWVVFAAAPVAWWVNRARANASPGRYVFLAAVALVFAVNYLRVLAGFMLDPVPGHVI
                     LPVAYAALLYGGYYFVAGQQGMREVKLLLVYAGHIMLMSAAVRMLDVRIFQSTAWGLL
                     ALAGLGVSMWKNDRMLAQSSLLVFGATAGKVLLYDLSGSPPLA"
     gene            complement(1760..>3571)
                     /locus_tag="VFK00_04360"
     CDS             complement(1760..>3571)
                     /locus_tag="VFK00_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET7592222.1"
                     /translation="WPWPRYRVAELVDRIAAGQPSAIAMDIVFPEADRTSLADIRKTF
                     KRDFGVDVSFNGVPPGLLDNDGYLGQEMARAGVVGAQYFYFDHVTRTPPARPGVGFDG
                     RVDLLSLDRAPGVLDSVDAIASETRISGFVNSRLDSDGVLRRLPLLIEHDGVIHASLA
                     LAATMHALGVDVARVESDPGGLSLKVGSHHVPIDRTGYALLRFNGASRLYGSLSAVDV
                     LAGRFKPADLRGKIVFIGSSAVGLHDVHRTAVDAGFSGLKIQSVMAQNILDGNAVRVP
                     AWGGKVALLVGLLIALAFSAMFIAGRGLLALATVGVVSGAALVATVAGVFVRSSVFVP
                     LAAPLLIAGSLLVLAFVARFVASQRRAQRWRKQLENARQVTIESMAAVAETRDPDTGA
                     HIKRTQHYVRAIARQLQRTGRYAELLSREYIDLLFLSAPLHDIGKVGVPDHILLKPGP
                     LTEDELKVMRRHAEFGRQIIHSTAEHIEGDNFLVVAGEIAATHHERWDGTGYPEGLRE
                     QDIPLSGRIMAVADVYDALISKRCYKDPFPHHAARSIMHSLRGSTFDPGVLDAFLEIE
                     SEIVQIAARFRDADVEKGRAKLAAMANPVRGGVGFTC"
BASE COUNT          673 a         1246 c         1166 g          486 t
ORIGIN      
        1 gccagcggcg gcgaaccgct caggtcgtag agcagcacct tgccggccgt cgccccgaac
       61 accagcagcg acgactgggc gagcatccgg tcgttcttcc acatcgacac gccgaggccg
      121 gcgagcgcga gcaggcccca cgcggtggac tggaagatcc ggacatcgag cattcgcacc
      181 gccgccgaca tcaacatgat gtggccggcg tagaccagca gcaacttgac ctcgcgcatg
      241 ccctgctgcc ctgcaacgaa gtagtagccg ccatacagca gcgccgcgta agcgacaggc
      301 aggatcacgt gtccaggcac cgggtcgagc atgaagccgg cgaggacccg caggtaattc
      361 acggcaaaca ccagcgccac cgccgccagg aacacatagc gtcctggcga tgcgttggcg
      421 cgtgcacggt tcacccacca ggcgaccggt gcggccgcga acacgaccca cggcgccatc
      481 tgcttcggga gcgcttcaag atagcctgcg tggaacagca ccagggacag gtagcaccac
      541 agcaggaatt caccgccggg caacggccgc cgcaggtatc ctcgcgcgcc ccagtagagc
      601 agcccgacgg ccgcgaggct ggcgaacgaa atccagggcg cgagcgccgg caggtgccgg
      661 tccagcacgt aatactgcaa cgcatagaac aacagcagcg ccggcaggtg cagtgccgcc
      721 gtggccgtgt ccatcggttc gcggcgccgc accgaaaacc aggccgtcgc gacgccgaag
      781 atcacgaact gcgccgcctg gaacaccagc gccgccaccc attggccgga ggcgtgcacg
      841 tgccaggccc cgtcgaaacc gaccagcgcg aggtaggcgg cgagcagata gatcaagcgg
      901 cgtccatgcc agatcgcgta cacgctgaat accacgcccc aggccgagta atacaccacg
      961 agatcggtaa tggagccggt cgaactcgcg agcaggaatg gtgcggagta ggaaccggcc
     1021 accgcgaaca gcgcatacag gtcgctgtcg aacgcccggc acagccacag cgacagcgcg
     1081 cacaccgcga cgacgaacaa accggccgcc ttcacgccga tcaactcgta atggaggtgc
     1141 gcgccgtaga tcgcgaggaa cagcatcacc acgccgcagg ccggcaggta gcccgcatag
     1201 cgccgccacg atccccgcaa tccgaaaccg gcgccgatca gcgctatccc gcccgccacg
     1261 accgacgaga cctgcacgac cggcgtcagc cagccactgt cgatgcccaa acgaatgacg
     1321 tatgcggccg ccagcaggaa agccagtgcc ccaccccaac ccaggatgct ggtgaccggc
     1381 gaaccgcgtg gcggcgccgg tacccgcgcg gcctgtgcgg gcgccacgtg ggcgggctcg
     1441 ggtgccgcgg gggtcgcagg cgctgccacc gcgggttgcg cgtgcggagg cacgtcccgc
     1501 aaccatatct cgcccggttg cggctgcccg ccttgcgctt gcggcggctc cggcggaacc
     1561 ttcggcacga accgcgcagc gaggcgctcc tcgagctgcg cgagacgtct ctccaccgcg
     1621 gccaaccgat cctcgacgct ctcccccgcc atgcccgtcg ctcccgtggt ttggagatcg
     1681 ttcgaactgg tgtcgtaccc cgccggcgtt tatagcaccc ccgcagggcc gccgcacccg
     1741 cgaagcgccg tggccagcgc taacaggtga aaccgacccc gccgcggacg ggatttgcca
     1801 tcgccgccag ctttgccctg cccttttcta catcggcatc gcggaagcgc gcggcgattt
     1861 gcacgatttc ggattcgatc tcgaggaagg catccagcac ccccggatcg aaggtgctcc
     1921 cgcgcaggct gtgcatgatc gacctcgcgg catggtgggg gaaagggtcc ttgtagcagc
     1981 gcttgctgat cagcgcgtcg tagacgtccg cgaccgccat gatgcggccc gagagcggaa
     2041 tgtcctgctc acgcaaccct tcgggatagc cggttccatc ccatcgttcg tggtgggtgg
     2101 cggcgatctc gccagcgacc acgaggaagt tgtcgccttc gatgtgttcg gccgtactgt
     2161 ggatgatctg ccggccgaac tcggcatggc ggcgcatgac cttcagttcg tcctcggtga
     2221 gcggaccggg cttgaggagg atgtggtccg gcaccccgac cttgccgatg tcgtggagtg
     2281 gcgccgacag gaacagcagg tcgatgtatt ccctgctcaa cagttccgcg tagcggccgg
     2341 tccgctgcag ttgccgtgca atggcgcgaa cgtagtgctg ggtgcgcttg atgtgcgccc
     2401 cggtgtccgg gtcgcgcgtc tcggcgaccg cggccatcga ttcgatcgtc acctggcgcg
     2461 cattctccag ttgtttccgc cagcgctgcg cccggcgctg gctggcgacg aagcgcgcca
     2521 cgaacgcgag caccagcaac gaacccgcga tcagcaacgg cgccgccagc ggaacgaaca
     2581 cgctgctgcg cacgaagaca ccggccacgg tcgcgacgag ggcagcgccc gacacgacac
     2641 cgaccgttgc caacgccagc agtccacgcc cggcgatgaa catcgcggaa aacgccagtg
     2701 cgatgagcag gccgaccagc agcgcaacct tgccgcccca ggcgggaaca cgcacggcat
     2761 tgccgtccag gatgttctgc gccatcacgg actggatttt caggccggag aaaccggcgt
     2821 ccacggcggt ccggtgcacg tcgtggaggc ctacggccga ggaaccgatg aatacgatct
     2881 tgccccgcag atccgccggc ttgaagcggc cggcaagcac atccacggca gaaaggctgc
     2941 cgtagagccg cgaggcgccg ttgaagcgca gcagcgcgta accggtccgg tcgatcggaa
     3001 cgtggtggga tccgaccttg agcgacaatc cgccgggatc cgattccacc cgcgccacgt
     3061 ccacgccgag tgcatgcatg gttgccgcga gggcgagact cgcgtggatg acgccgtcgt
     3121 gctcgatcag caagggcaac cgtcgcaata cgccgtcgct gtccaggcgg ctgttgacga
     3181 agccgctgat acgggtctcg gaggcgatgg catccacact gtcgaggacg cccggcgccc
     3241 ggtcgaggga cagcaggtcc acccggccgt cgaagccgac cccggggcgc gccgggggcg
     3301 tgcgggtgac gtggtcgaag tagaaatact gcgcccccac gacaccggcg cgcgccatct
     3361 cctggcccag atagccatcg ttgtccagca agcctggcgg cacgccgttg aatgacacat
     3421 cgaccccgaa gtcgcgcttg aaggtcttgc ggatgtccgc cagcgaggtg cgatcggctt
     3481 cggggaacac gatgtccatc gcgatcgccg aaggctggcc ggccgcgatc cggtccacca
     3541 gttccgccac ccggtagcgc ggccatggcc a
//
LOCUS       DASSNH010000123        20424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1399218, whole genome shotgun sequence.
ACCESSION   DASSNH010000123 DASSNH010000000
VERSION     DASSNH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 20424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20424
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399218"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFK00_04365"
     CDS             complement(<1..183)
                     /locus_tag="VFK00_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7592223.1"
                     /translation="MPQVTMRQMLEAGVHFGHQTRYWNPKMAPFIFGARGKIHIINLE
                     ATVPKFTDAMNYLSGLA"
     gene            486..1250
                     /gene="map"
                     /locus_tag="VFK00_04370"
     CDS             486..1250
                     /gene="map"
                     /locus_tag="VFK00_04370"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7592224.1"
                     /translation="MPVIPKTPEEIEGMRVAGRLAAEVLAVLKEHVKPGVTTEELDQV
                     AYRHIVDVQNAIPANVGYRGFPKTLCTSVNHVICHGIPSPGKVLKDGDIVNCDVTVIK
                     DGWHGDTSRMYFVGTPSVLAKRLVDTTYEAMMKGIGQVRPGATLGDIGHAIQQHAESA
                     GYSVVREYCGHGIGRIYHDDPQVLHYGRAGEGLRLEKGMTFTVEPMVNAGKRHTRALP
                     DGWTVVTKDHSLSAQWEHTVAVTDDGYEILTPWPDA"
     gene            1734..4127
                     /gene="glnD"
                     /locus_tag="VFK00_04375"
                     /pseudo
     CDS             1734..4127
                     /gene="glnD"
                     /locus_tag="VFK00_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
     gene            4045..5043
                     /gene="dapD"
                     /locus_tag="VFK00_04380"
     CDS             4045..5043
                     /gene="dapD"
                     /locus_tag="VFK00_04380"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET7592225.1"
                     /translation="MPACACTTRASRPSATRSRTSSSSATAAMRRSTRSCRRPCARRC
                     SGASRPAAWSPTRESTMPDLQHAIDAAWEQRANLREADAGELRPTLDKVLDALEGGEL
                     RVAEPDGQGGWTVHAWIKRAILLYFRAHDPHVLDGGPMAAFDKVPLRFAGAGAAAFRM
                     AGARIVPGALVRRGAFVGRDAVLMPSYLNIGAHVGPGAMVDTWATVGSCAQIGARVHL
                     SGGVGIGGVLEPLQASPTIIEDDCFIGARSEVVEGVVVEHGSVLGMGVFLGQSTRIYD
                     RATKTISYGRVPAGSVVVAGTLPAPDGSHGLYAAIIVKRVDEKTRAKTSINELLRA"
     gene            complement(5085..6752)
                     /locus_tag="VFK00_04385"
     CDS             complement(5085..6752)
                     /locus_tag="VFK00_04385"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7592226.1"
                     /translation="MKPLCLAVATICAALALSGCGGSSGSSTQPPAQPPPPPSPPPPP
                     PPPPPPPPPPPNVDPLYRASGASPFGPNCDGEPVTGTLYVDAEVEPSLAIDPRNPMAM
                     AAVWQQDRWSNGGARGIVAAVSPDGGHTWAQHPIPLTRCAGGTPANGGDYPRASNPWI
                     TVAPDGTLNQLALGFDGGALTPGSVSAVLTSSSVDGGFSWGPTKTLIRDSGGQFFNDK
                     GAITADPVTPQYVYAVWDRLTATTGPTVFTRSSDGGQTWATVRAIFDPGPNNQTISNA
                     IVVLPDGRLVDFLLEIDGTNAGQFHSYLEVIRSDDQGATWSAPVRIAENLSVGTRDPD
                     TGTPVRDSSLVPEIAAGPGGELYVVWQDSRFTGGARDAIALSVSTDGGSSWSAPLRIN
                     ADLSTPAFSPQVHVRADGMVAVSYYDFRDDTGDPVTLYTGYWLTRSLDLSTWEENRIA
                     GPFDLALAPYTTSPGAGGYFLGDYQALASVGTTFMPMFVQTATTTDPTDVYFAPQVSA
                     MAAIQGATHVTAQSASAYSVNDALRARASDNIVRAMRARIPNWREHP"
     gene            7035..7394
                     /locus_tag="VFK00_04390"
     CDS             7035..7394
                     /locus_tag="VFK00_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HET7592227.1"
                     /translation="MTGATLYGLPNCDTCRKARNWLDRFGVDYRFVDYRSDPVPATTL
                     KDWANRLGGWEKLVNKSSTTWRNLLPQRKNPGSDPEWTLLLKEYPALVRRPVVVREDG
                     AVSVGFSDNGFKKTFGR"
     gene            7402..8466
                     /locus_tag="VFK00_04395"
     CDS             7402..8466
                     /locus_tag="VFK00_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET7592228.1"
                     /translation="MPDPRARRYLKNLRVERDNEALYARLAAAASDTRLARAYLRIAE
                     GERRNAAFWEARLRDVGAAVPPPRPRARVRVLGALAKWFGTGFVMPTVVRLEHADHLE
                     TPVDRTGHRDHFVPADARSARGHRHRAGGGNTLRASVLGANDGLVSNVSLVMGMAGAA
                     TGNHAVLLAGLAGLVAGSCSMALGEWLSVNSSREFYQAQITERAERLAVAPEEGVRHI
                     AGIYRDKGMGRAKAEHLAEHLSETPRTALDTVVREDLGVDPSELGGSAWAAAISSFCF
                     FAFGAIFPVAPYFFVGGTVAMFASMGTTFVGLCLIAVGTSLFTGRSLAFSIARQFAIT
                     AAAAAITFGVGHALGLAITG"
     gene            8513..9649
                     /gene="dapE"
                     /locus_tag="VFK00_04400"
     CDS             8513..9649
                     /gene="dapE"
                     /locus_tag="VFK00_04400"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET7592229.1"
                     /translation="MSEVFDLTCELIRRRSVTPDDAGCMALLAARLERVGFRCEHLHY
                     GEVVNLWATHGEGEPVLLFLGHVDVVPSGPEADWHSPPFEPTVRDGKLYGRGAADMKS
                     GVAAMAVAMERFVAAHPGHPGTVALLLTSDEEGPDNLDGVRRVARRFRETGQRIDWCV
                     VGEPTAKAELGDLIRVGRRGSLSGVLTVRGVQGHVAYPQLARNPVHLFAPALAELAAV
                     RWDEGNGDFPPTSFQVSNIGAGTGAYNVVPGSLVAHFNFRFGTASSAEGLRSRTEAIL
                     RKHELEYELDWNLSGEPFLSPAGGRLRETVIDLCRELRGVEPEQSTGGGTSDGRFIAP
                     LGAEVIEVGLVNASIHKIDECAALDELERLPGIHFAIAERLLRG"
     gene            complement(9659..10219)
                     /locus_tag="VFK00_04405"
     CDS             complement(9659..10219)
                     /locus_tag="VFK00_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HET7592230.1"
                     /translation="MRLKSSAVAPTLALKDVHGQPVALGGASGRRTLVCFFRDAACPF
                     CNFRIYELTRHHASLAALGLEVIAVFASTPEEVRRFVARKPRPFVVVADPTSRAHDAY
                     GIEHSFWRKWKGVFSRIPTLLKGLRIVGAAGLATSNLMPADFLIDEHGRIAEAYYGRD
                     AGDRIPFERIELFLARGLLKRVAVAA"
     gene            complement(10356..11105)
                     /locus_tag="VFK00_04410"
     CDS             complement(10356..11105)
                     /locus_tag="VFK00_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET7592231.1"
                     /translation="MLLPAACARGSHGIESHDVTFDNVHFRVVTIDLARAKLGLHWRD
                     PATGKPFASIHALQLWGARNGQRLLFAANAGIYDSHGEPLGLHVENGRTVTPLNRAHG
                     DPAAGNFSLLPNGVFAIDAAGHASVQTTQAFAKSGTEPRIATQSGPMLVIGGNINPEF
                     VADSDSMKWRSGVCAPQPDQAVFVVSAGPVNFHTFASLFRNKLGCRDALYLDGTLSQI
                     YADGNYYGAPAFMVKPYAGIFAVFVAADDGR"
     gene            complement(11256..13652)
                     /locus_tag="VFK00_04415"
     CDS             complement(11256..13652)
                     /locus_tag="VFK00_04415"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET7592232.1"
                     /translation="MRWVRRVIVAAIIVLVITFAFGWRLLAGSRPAADGTLRVQGLAA
                     PVTIDRDVDGVPTITARDRNDLAWALGFAHGQERFFQMDLQRRSAAGELAALVGKAAV
                     ASDEDHRQHRFRALARQQLAHLAPEQRKLVHAYTAGVNAGLAHLSVRPWEYLLLRARP
                     QPWTDGDTVLTIDAMYFDLNQNGDNARELNIARLRAELPDALADFLLAPAPRWEAPVQ
                     GQASAAVPVPAASVFDLRTHATNKVALTRGTRAVGRAGAANTGVGSNAFAVGGALAGG
                     AALLANDPHLHLRVPNIWYRARMRYPDPADPSRMIDLNGVTLPGAPAMVIGSNGHVAW
                     GFTNSGGDWMDWVRVIRDPADPSRYKTPDGWATITRHDEVIHVKGAADVHFTVEDTIW
                     GPMMAEAPDGTPLALAWIAQTPQAVNLDLMKLETATSVDEALSLAPGMGIPPQNLVVT
                     DAQGHIGWTIAGSAIPRRTGFDPSLPADWSKPGTGWSGFASHAQYPRVEDPADHRIWT
                     ANQRIVSDDALELLGDGGYDPGARAQQIRDDLAAHDQFIPRDMLAIQLDDRALFLARW
                     QKLLLDVLSGADDPGTRALAPFVRNWQARATADSVGYRIVRMFHDQVRENVLAPFAAR
                     ASLKFKDFEWPTSAVGEYAAWTLVTQRPSWLLDPQYQDWHALLEASARQVADALAEEP
                     GRLADKTWGRRNAARIDNPLSVVLPGWLARFADMPHDPLPGDNDMPRVLHPSFGASMR
                     LDVQPGNEAHGILEMPAGQSDNPLSPWFGKGHEAWVHGKPTPLLPGPAAWHLTLEPGR
                     "
     gene            complement(13937..14713)
                     /locus_tag="VFK00_04420"
     CDS             complement(13937..14713)
                     /locus_tag="VFK00_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HET7592233.1"
                     /translation="MSRTDWNDYEWRLVHGTPQPPALHMAIDEVLTDEVAARRRPPTL
                     RVWEWSSSCVVIGRFQSLRNEIDPDGARRHGIEVVRRISGGGAMFIEPGNTITYSLYA
                     PEALVAGMSFQESYEFMDGWVIDALHGLGIDAWYQPLNDITSPSGKIAGAAQTRRGGA
                     VLHHVTMAYDIDAAKMLEVLRIGREKLSDKGTASAAKRVDPLRSQTGLPREEIIERMI
                     DTFRAAHGLRRDRLSPQELERAEALAEAKFASAAWTGIVQ"
     gene            complement(14710..14994)
                     /locus_tag="VFK00_04425"
     CDS             complement(14710..14994)
                     /locus_tag="VFK00_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HET7592234.1"
                     /translation="MHGEYKMPGGKLVVADLEVRDGKLFRVRISGDFFLEPDTALTLI
                     DVALEGLPAISSGEDLAAAVRVTLGPHVGMYGVSPESIAVAVQRALEAAS"
     gene            15111..15668
                     /locus_tag="VFK00_04430"
     CDS             15111..15668
                     /locus_tag="VFK00_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592235.1"
                     /translation="MENFRDLSALCGVLRLMLEESPREERQAAFADALQKALGFASEP
                     AGLYAATVFHENTGESPDRVLANGSWLTRTWVRGEQQGNVGAWLSTTTERWRFDEDLT
                     YRYTFERIDTGLSSGPFFQSSYTKPRTNEQRGIWAPPDTQQDQFELFVMPFGGVARPL
                     PFEWVEKEVYGYAACMIGGTRFTRE"
     gene            complement(15725..17965)
                     /gene="parC"
                     /locus_tag="VFK00_04435"
     CDS             complement(15725..17965)
                     /gene="parC"
                     /locus_tag="VFK00_04435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET7592236.1"
                     /translation="MTALQSNFEQIPLREYAERAYLDYSMYVVLDRALPFIGDGLKPV
                     QRRIVYSMSELGLAATAKPKKSARTVGDVIGKFHPHGDSACYEAMVLMAQPFSYRYPL
                     IDGQGNFGSPDDPKSFAAMRYTESRLTPIAELLLGELAMGTVDWTPNFDGTLKEPSWL
                     PARVPHVLLNGGTGIAVGMATDIPPHNLREVVSACIHLLDEPSASIADLCGHIRGPDF
                     PTAAEIITPRNELAAMYATGTGSVRCRAVYTEERGNVVLTALPHQVSPAKVVEQIAAQ
                     MRAKKLPMLEDLRDESDHENPVRLVLIPRSNRVDTDAMMQHLFATTDLEKSFRVNLNM
                     IGLDGRPQVKTLKQILDEWLRFRTDTVTRRLQHRLEKVERRLHLLEGLRIAFLNLDEV
                     IRIVRHEDEPKPALIKRFKLSEEQADYILETRLRQLARLEEMKLNTERDELEEERAKI
                     DVTLKSKARLRTLIKQELKADADKFGDARRSPLVERAAAQALDESQLVTAEPVTVVLS
                     KKGWARAAKGHDVDAAALSYREGDAFLASMRGKSSQQAAFIDSTGRSYSTPAHTLPSA
                     RGNGEPLTGRFTPSPGARFDALAIGDSADRLILATDSGYGFLTRFEALLANKKAGKQV
                     INMDTGACVLAPAGVADPARDRIVAATTAGHLLMFSVAELPELDRGRGNKLIEIPKAK
                     LQSGEERLAGIAVVAEGSGEVTLYAGQRKLTLKWADLVEYGGNRAQRGGLLPRGLRRV
                     DRIESA"
     gene            complement(18036..18908)
                     /locus_tag="VFK00_04440"
     CDS             complement(18036..18908)
                     /locus_tag="VFK00_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET7592237.1"
                     /translation="MKTLRRFCFALAFVLIASTPALADPALWVAKGPSATVYLFGTVH
                     VLPKGAHWHYPALDEALAASEKLYVEEDDDSPLDMQMLVLEYGMDLQHPLSDKLDATD
                     QARLAAAAEAIGVAGGAATLNAMKPWLAALTIAVAPIVKAGYDPKSGADKQLDHAFTA
                     AGKSVGAFETAEAQIKLLANLSPALQLDLLRNALDDYAKGPAQIEALIRDWQAGNVAA
                     IATVVNGGMHEHYPELYKALLVDRNRAWAGRLAGLLEQHGTVFVAVGAGHLAGPDSVQ
                     KQLAKLGIATQRVH"
     gene            complement(19017..>20424)
                     /locus_tag="VFK00_04445"
     CDS             complement(19017..>20424)
                     /locus_tag="VFK00_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7592238.1"
                     /translation="WTAEFLASSAPIAVWWASRYTRGTAWLWLLFAMAILPTGSRLGL
                     ASLLVGAAIYAWTSQRGHGDSRIPRNQAGFFLTAVVALGSLALLLRDDGYAAALLRWN
                     DWHSVLSRVHLWQASLHLIAARPVHGYGLDHFALVLPDGLRAVAAPLDAVARSRMPDL
                     LTAHAHNDFLETAVETGLPGGLLLLASCALGLHAARRALDSIPPLPLTKSPDEPCTPN
                     RTRVATAVPALAASLGVLFMLAWASAPLHTPATALLFWLVLGCVAGLSEAAPAGALCR
                     AHAGRRAWQRAGGAAAVCAMLAVTGWSSHRAFALLTENREAATAAAMAPIAPPRVAVR
                     LYEKALARAPWDHESGVALASLLIADRQPAAALRVLDRADAWSQSRESWLARARALLL
                     EHDAEGALRTMQHATAALPDFLRAQALRARLAARLGKTPEAKAAWHQVLLSPQRSARA
                     QQIVLEAVQASRRQAQAP"
BASE COUNT         3085 a         6793 c         7258 g         3288 t
ORIGIN      
        1 cgcgaggccc gacaggtaat tcatcgcgtc ggtgaacttc ggcaccgtcg cttcgaggtt
       61 gatgatgtgg atcttgccgc gggcgccgaa gatgaagggc gccatcttgg ggttccagta
      121 gcgcgtctgg tggccgaaat gcacgccggc ttccagcatc tggcgcatgg tgacttgggg
      181 catgatgatg actccgatgg ggaacccgct ccgcttgcgc ggcgtgcgcg gccgtcgtgg
      241 acgggattgg cgcgacaggt tccggggttg ggcttccgca ggccccgcga caacgaccct
      301 caatgaggga ccccggtgcc ggtgcatcac tatgggtgca ggcctgcgtg tgtattcgcc
      361 ggtggcgccc ggcgtgggcg gtctgcgttc gcagagccgg taaagtgtac cggcagcggc
      421 cgcccgaggc aagcgggacg ccgcgcggct gcaggaccgg tgcgaatgga ggataatggc
      481 gcgctatgcc tgtcattccc aaaacccctg aagaaatcga ggggatgcgc gtcgccggcc
      541 gcctcgcggc cgaggtgctc gccgtcctca aggagcacgt caagccgggc gtcacgaccg
      601 aggaactcga ccaggtcgcc tatcgccaca tcgtggacgt gcagaacgcg atcccggcga
      661 acgtcggcta ccgcggcttc cccaagacct tgtgcacgtc ggtcaaccac gtgatctgcc
      721 acggtatccc gagtcccggc aaggtcctga aggacggcga catcgtcaat tgcgacgtca
      781 ccgtcatcaa ggacggctgg cacggcgaca cctcccgaat gtatttcgtg ggaacccctt
      841 cggtgctcgc caagcggctg gtcgacacca cctacgaggc gatgatgaag ggcatcgggc
      901 aggtgcgccc gggcgcgacc ctcggcgaca tcggccacgc gatccagcag catgcggagt
      961 ctgccggcta ctcggtggtg cgcgaatatt gcggacacgg catcgggcgg atctaccacg
     1021 acgatccgca ggtgttgcac tacggccgcg ccggcgaggg gctgcggctg gagaagggca
     1081 tgaccttcac cgtcgagccc atggtcaatg ccggcaagcg ccacacccgt gcgctgccgg
     1141 acggctggac cgtggtcacg aaggatcatt ccctgtccgc gcagtgggag cacacggtcg
     1201 ccgtcaccga cgacggctac gagatcctga ccccttggcc cgacgcgtaa tttttgcggc
     1261 cttccctgat cgcccggcat caggacggta cgatgcactg accgctctgg cgtgctgaac
     1321 tccggaacgg ccacccatgg cctgactttt cgatattttt ttgcgatcat ccctgatcgc
     1381 cgcctcgtcg caaagtccgc gtttgtcggg ccgcaggcaa cccggcagca ccagaacggc
     1441 cttccgcggc ccccgatcag aaccacctcg ggggcaagct ccgacttttc gcaacagcgg
     1501 cttcggatgg gacatcggtg actgctctcc agacgccgtc gcagggaaac ccgtccagca
     1561 ctcgtgcggt tccaccgctg ccgcgcctcc cgcccgcgct gccgctggcg ggggtttcgc
     1621 gcgacgcgcg cgcggcgctg cgccagttgc tggacgacca tgctcgcgcg ctgacgatcg
     1681 ccttccgcga gggcgccgat gcgtcgttgc tcgcacgcac gcgcgcacgc gccgtggaca
     1741 tggtttgcgc gcacgtgtgg tcggcctacg ttggcgaaac cgacgcgatg gcgctgttcg
     1801 cggtcggcgg cttcgggcac ggattgctgt tcccgtattc ggacgtcgac ctcctggtgt
     1861 tgcacgacgc ggatccggag gcggcttccg cccgggccct cgaggcgttt ttcacgtgcc
     1921 agtgggacat cgggctcaag ccggggcagg ccgtgcgcac gccggggcaa tgccgcgaca
     1981 tcgcggccgc ggacgtcggc gtgttcacca gcctgctcga tgcgcgccgg ctggcgggcg
     2041 ccgagcggct cgaacgggag ctgcgggcga tcaccgccga tccgtcgctg tggactccgg
     2101 acgcatacct cgcggccaag ctggaggaac gcgcccagcg ccacgcgcgt ttcgacgaca
     2161 ccacgcacaa cctggagccc gacctcaagg acggccccgg cggcttgcgc aatctcgacc
     2221 tgatccgctg gcttggcaag cggctggtcg gggtcgatga cctcgacggg ctggtgcgcg
     2281 cgaacctcct cgaggcgagc gaacgcgacg ccttgcgcaa cgcacgcacg gtgctgcgcc
     2341 gcgaccgcta cgggctgcac ctggaggccg gtcgcgccga ggagcggttg ctgttcgact
     2401 ggcagcgcgc ggtggcggcg cgcctcggct acgaggacgc caacaccgtc ggcaacgaag
     2461 cgatcgaacg cttcatgcag gactatttcc gcgcggccgg cgccaccgag cggctgctgg
     2521 tgcagttgct cgagcgcctc caggaatacc tcaatccatt gccgccgccg cgcgatctcg
     2581 acgagcactt cgtgctgcgc gggagccgca tcgaactgcg cgacccggac tggctgctgc
     2641 gcgcgccgga ggggttgatc gaggtgttcg ccaagcgctt cgaggtggag ggctgcagtg
     2701 gcacgaccgc ggccaccatg cgcgcggtgg aacatgcgct ggtcgcgcac ggtacgtcgc
     2761 tgtcgcgaag cccgccggta ctgtccgcgt tcctcgcgtt gctgcgccgc ggcgcggatg
     2821 cggtgggggt gatcgatgcg ctgaaccggc atggcatcct cgcggcgatc ctgccgccgt
     2881 tcgcgcgcgt ggtggggcgg atgcagtacg acctgttcca cgtgtacacg gtcgacgaac
     2941 ataccctgcg ggtgctgcgc aacatcgcgc gctacgcgca gccggaagca cgcgctgccc
     3001 tgccgctcgc ctgcgaggcg ttcgcgcgta ttgcgaagcc ggagctgctg ctgctggccg
     3061 ggatcttcca cgacatcgcg aagggacgcg gcggcgatca ttccagcctc ggcgaaaccg
     3121 aggcgcgtgc gttctgccgc aacctcgggc tggacgaggt cgacatcgac gaggttgcat
     3181 ggctggtgcg ctggcacctg ctgatgagcg tcaccgcgca acgccaggac atcgccgacc
     3241 cggaggtggt gcaccgtttc gcggtgcagg tgggcgactg ggcgcggttg gacctcctgt
     3301 atctcctgac catcgccgac atcgccggga ccagtcccag gctctggaat tcctggaagg
     3361 accgcctgct cgcggacctg tacgtcgccg cgcgatacgt gttgcggcgc gacctcgaac
     3421 gccccgcgcg cgccgaaatc aggattcgcg aggcgcgcga gcgcgccctg ggactgctgg
     3481 tcgaacgcgg gttcgagccg accgcggcgg atcgcgcgtt ggggcagttc ccggataccg
     3541 ccctgctgcg ccagcgcgcg gaggtgctgg cgtggcaggg ggcggcgttg ctggacgatc
     3601 ccatggcgcg gacggtggtg gccgtgcgca atccggacgt gcgcgggggc accgaggtgt
     3661 tcgtgtgcac gccggatcgc gacggcgtgt tcgccgcggt ggccgcaacc ctcgatcggc
     3721 tgcgactcga cgttgtcgcc gcgcgtgtga tgacctcccg cgacggccgc gcgctcgata
     3781 cgttcacggt gctggaagcc ggcacgcaat tgcccgcgcc ggccgaacgc gcaggtgcat
     3841 tgcgcgacgc gctgcagcgc gcgctgggcg cggcgaccct gcgcacccaa cccgcgaggc
     3901 gcagcctcac ccgtcgcctg cgtcacttcc agcgtccacc acgcatggat ttcgcgggcg
     3961 acgaaggccg cccgctgtcg cggctggctc tggtgtgcag cgaccggccg ggactgctgg
     4021 tcgcgatcgc gcaagctttc ctggatgccg gcgtgcgcgt gcacgacgcg cgcatcgcga
     4081 ccttcggcga ccaggtcgag gacttcttcg agctcagcga ccgccgcaat gcgccgctcg
     4141 acgcgaagct gcaggcgacc ctgcgcgcgg cgttgcagcg gcgcctcgcg cccggcagca
     4201 tggtcgccga ccagggaatc gaccatgcct gacctgcaac acgcgatcga cgctgcatgg
     4261 gagcaacgcg caaacctgcg cgaagcggac gccggcgaac tgcgcccgac cctggacaag
     4321 gtcctcgatg cgctcgaggg cggcgagctg cgggtcgcgg aacccgacgg gcaaggtggc
     4381 tggaccgtcc acgcctggat caagcgtgcc atcctcctgt atttccgggc gcacgacccg
     4441 cacgtgctgg acggcggccc gatggcggcg ttcgacaagg tgccgttgcg cttcgcgggt
     4501 gccggcgcag cggcgttccg catggcgggc gcacgcatcg tgccgggtgc gctggtccga
     4561 cgcggggcgt tcgtcggccg cgatgcggtg ctgatgccga gctacctcaa catcggtgcc
     4621 cacgtcgggc cgggcgcgat ggtggacacg tgggcgacgg tcggatcgtg cgcacagatc
     4681 ggtgcccggg tgcatctttc cggcggcgtc gggatcggcg gcgtgctcga gccgttgcag
     4741 gcttccccga cgatcatcga ggacgactgc ttcatcggcg cgcgctcgga ggtggtcgaa
     4801 ggggtggtcg tcgagcacgg cagcgtgctc gggatgggcg tgttcctggg acaatcgacg
     4861 cgcatctacg atcgcgcgac gaagacgatt tcctacggtc gcgtgccggc gggcagcgtg
     4921 gtggtggcgg ggacgctccc ggcgccggat ggttcgcacg ggctgtatgc cgcaatcatc
     4981 gtcaagcgcg tggacgaaaa gacccgcgcg aagacttcga tcaacgaatt gctgcgcgcg
     5041 taacaggggg gatgtgcggc gcagcggttg gcctttgccg atggctacgg gtgctcgcgc
     5101 cagttcggaa tacgcgcccg catcgcgcgc acgatgttgt cgctggcgcg cgcccgcaag
     5161 gcgtcgttca cgctgtaggc cgacgcggac tgcgcggtga cgtgcgtggc cccctgtatc
     5221 gccgccatcg cggacacctg cggcgcgaaa tagacgtcgg tcgggtcggt cgtggtggcc
     5281 gtctgcacga acatcggcat gaacgtcgtt ccgacgctgg ccaacgcctg gtagtcgccg
     5341 aggaagtaac cgccggcgcc cggtgacgtc gtgtacgggg cgagggcgag gtcgaacggg
     5401 cccgcgatgc gattttcctc ccaggtcgaa aggtcgagcg aacgcgtgag ccagtagccc
     5461 gtgtacaagg tcaccggatc gccggtgtcg tcccggaagt cgtagtagct gaccgcgacc
     5521 atgccgtccg cgcgcacatg cacctgcggc gaaaatgcgg gcgtcgagag atcggcgttg
     5581 atgcgcagcg gagcagacca gctcgacccg ccgtcggtcg aaacggagag cgcaatggca
     5641 tcccgcgcac cccccgtgaa gcgtgaatcc tgccacacga cgtacagttc gccgcccggc
     5701 ccggcggcga tttccggcac cagcgagctg tcgcgcaccg gcgtgcccgt gtccgggtcg
     5761 cgcgtgccaa ccgacaggtt ctcggcgatg cgcaccggcg ccgaccacgt cgcgccctgg
     5821 tcgtcggagc ggatcacctc gagataggag tggaactgcc ccgcgttcgt gccatcgatc
     5881 tcgagcagga agtccaccag gcgaccgtcg ggcaggacga cgatggcatt gctgatcgtc
     5941 tggttgttcg gcccgggatc gaagatcgca cgcaccgtcg cccaggtctg cccgccatcg
     6001 ctgctgcggg tgaagacggt gggtccggtg gtggccgtga ggcgatccca gacggcatac
     6061 acgtactgtg gcgtgaccgg atcggcggtg atcgcaccct tgtcgttgaa gaactggccg
     6121 ccgctgtccc ggatcagggt cttcgtcggg ccccaactga atccgccgtc gacggatgaa
     6181 ctggtcagca ctgcgctcac cgaacccggt gtcaatgcgc cgccgtcgaa gcccagcgcg
     6241 agctggttca gcgtgccgtc cggggccacg gtgatccaag ggttgctggc gcgcgggtaa
     6301 tcgccgccgt tggcgggcgt gcccccggcg catcgggtca ggggaatcgg gtgttgcgcc
     6361 caggtgtggc caccatcggg cgacacggcg gcgacgatcc cgcgcgcgcc gccgttcgac
     6421 cagcgatcct gttgccagac cgcggccatc gccatcgggt tgcgcggatc gatcgccagc
     6481 gaaggttcga cctcggcatc gacgtagagg gtgccggtga ccggttcgcc gtcgcagttc
     6541 ggcccgaacg ggctggcgcc cgaggcgcgg tacagcgggt cgacgttcgg cggcggcggt
     6601 ggaggcggcg gcggcggcgg cgggggtggc ggcggcgaag gaggcggagg cggctgtgcg
     6661 ggcggttggg tggaactgcc cgaagatccg ccgcacccgg acagtgccag cgccgcgcaa
     6721 atggtcgcaa cagccaagca cagcggtttc atgaccgtca ctgccctgcc gaagaccgac
     6781 gtggaaaagt gtagtcgaaa ccttgcgcgg cagcgttctc gagcgggcag gcgtccggag
     6841 gtttggcgcg attcgtcggc cggagctctg ggaaagcgtc cggtcgatga tgccgtggat
     6901 agccgatgaa agacgagacg gccgcgctcg gcagatgttt cagggcagcg atggcagaat
     6961 gggcgccgac gtcggctacc cgattggcgc ccaatcgagt catcgcggac ggacttcacc
     7021 ccgaggtgcc ttgcatgacc ggtgcgacgc tctacggatt gcccaactgc gacacatgcc
     7081 gcaaggcgcg caactggctg gatcgcttcg gcgtcgatta caggttcgtc gactatcgct
     7141 cggatcccgt gcccgcgacc acgctcaagg actgggcgaa ccgcctcggc ggctgggaaa
     7201 agctggtcaa caagtcctcg acgacctggc gcaacctgct gccgcaacgc aagaaccccg
     7261 ggagcgaccc ggaatggacg ctgctgctca aggagtatcc ggcgctggtt cgccggccgg
     7321 tcgtcgttcg cgaagacggc gcggtcagcg tcggcttcag cgacaacggc ttcaagaaga
     7381 cgttcggcag gtgatgcgcg catgcctgat ccccgcgcgc gccgatacct gaagaacctg
     7441 cgtgtcgagc gcgacaacga ggcactgtac gcccgcctcg cggccgcagc gtcggacacg
     7501 cggctggcgc gcgcctacct gcgcatcgcc gaaggcgagc gccgcaacgc ggcgttctgg
     7561 gaggcgcgcc tgcgcgacgt cggtgcggcg gttccgcccc cgcggccacg cgcgcgggtg
     7621 cgggtgctgg gcgcgctggc gaaatggttc ggtaccggct tcgtgatgcc gacggtggtg
     7681 cggctggagc acgccgacca cctggaaacg ccggtcgacc gcaccggcca ccgcgaccat
     7741 ttcgtgcccg cggacgcgcg cagcgcgcgc gggcaccgcc atcgggcggg cggcggcaat
     7801 accttgcgcg cttcggtact cggtgccaac gacgggctgg tgtcgaatgt cagcctggtg
     7861 atgggcatgg ccggcgccgc gaccggaaac cacgcggtgc tgctggcggg cctcgccggt
     7921 ctcgttgccg gttcgtgttc gatggcgctg ggcgaatggc tgtcggtcaa cagttctcgc
     7981 gagttctacc aggcgcagat caccgagcgc gccgaacggc tggcggtggc gcccgaggaa
     8041 ggcgtgcgcc acatcgccgg catctaccgc gacaagggca tgggccgcgc caaggccgag
     8101 cacctggccg agcacttgtc cgaaacgccg cggaccgcgc tggacacggt ggtgcgcgag
     8161 gaccttggtg tggatccgtc ggagctgggc ggctcggcgt gggcggccgc gatttcgtcg
     8221 ttctgcttct tcgcgttcgg ggcgattttc cctgtggcgc cgtacttctt cgtgggcggc
     8281 acggtcgcga tgttcgccag catgggcacg acgttcgtgg gcctgtgcct gatcgcggtg
     8341 ggcacctcgc tgttcaccgg ccgcagcctg gccttctcga tcgcgcgcca gttcgcgatc
     8401 accgcggccg cggccgcgat taccttcggc gtcggccacg cgctgggact cgcgatcacg
     8461 ggttgagccc gcgttgttat gctgcgcgtc gtcgttgccg cgccgatgtg ccatgtccga
     8521 agtcttcgac ctcacctgcg aactgatccg ccgccgttcg gtgacgccgg atgatgcggg
     8581 gtgcatggcg ctgctcgcgg cgcggctgga acgcgtgggc ttccgctgcg agcacctgca
     8641 ttacggcgag gtcgtcaacc tgtgggcgac gcacggagag ggcgagcccg tgctgctgtt
     8701 cctcggacat gtcgatgtgg tgccgagtgg tcccgaggcc gactggcact cgccgccatt
     8761 cgagccgacc gtccgcgacg gcaagttgta cgggcgcggt gcggccgaca tgaagtccgg
     8821 cgttgccgcg atggcggtgg cgatggaacg gttcgtggcc gcccatccgg gtcatccggg
     8881 aaccgtcgcg ctgctcctga ccagcgacga ggagggcccg gacaacctcg atggcgtgcg
     8941 ccgcgtcgcg cggcgttttc gcgaaaccgg ccagcgcatc gactggtgcg tggtgggcga
     9001 gccgaccgcg aaggcggaac tgggcgacct gatccgggtc ggccggcgcg gttcgctctc
     9061 cggcgtcctc accgtacgcg gcgtgcaagg ccacgtcgcc tacccgcagc tcgcccgcaa
     9121 tcccgtccat ctgttcgcgc ccgcgctggc ggaactcgcc gccgtgcgct gggacgaggg
     9181 caacggtgat tttccgccga cctcgttcca ggtttccaat atcggtgccg gcaccggcgc
     9241 atacaacgtg gttccgggct cgctggtcgc gcacttcaat ttccggttcg gcaccgcaag
     9301 cagcgccgag ggcctgcggt cacgcaccga ggcgatcctg cggaagcacg aactggagta
     9361 cgagctcgac tggaatcttt ccggcgagcc gttcctgtcg cccgcgggcg ggcgcctgcg
     9421 cgaaacggtg atcgacctgt gccgggaact gcgcggcgtc gaacccgagc agagcacagg
     9481 cggcggcacc tccgatggtc gcttcatcgc cccgctcggt gcggaagtga tcgaggtggg
     9541 actggtcaac gccagcatcc acaagatcga cgagtgcgcc gcgctggatg aactggagcg
     9601 cctgccgggc atccacttcg cgatcgccga acgcctgctg cgcggatgag ccgggcactc
     9661 aggccgcgac cgcgacacgc ttcagcaggc cccgcgccag gaacaattcg atccgttcga
     9721 acggaatgcg gtcgccggca tcgcggccgt agtaggcctc ggcgatcctg ccgtgctcgt
     9781 cgatcaggaa atccgcgggc atcaggttgc tggtggcgag gccggccgcg ccgacgatgc
     9841 gcaggccctt cagcagcgtg ggaatgcggc tgaagacgcc tttccatttg cgccagaacg
     9901 aatgctcgat gccgtacgcg tcgtgcgcgc gggaggtcgg atcggcgacc accacgaacg
     9961 gcctcggctt gcgtgccacg aaccggcgca cttcctcggg cgtcgatgcg aacaccgcga
    10021 tcacctcgag gccgagcgcc gcgaggctgg cgtggtggcg ggtgagctcg tagatgcgaa
    10081 agttgcagaa cgggcaggcc gcgtcgcgga aaaagcagac cagcgtacgg cggccgctcg
    10141 cgccgccgag cgcgaccggt tgcccgtgca cgtccttgag cgccagcgtg ggcgccacgg
    10201 ccgatgactt cagtctcatg tgttttcccc tgtggttggc cgcccccgcg gcactcggcg
    10261 accgggttgc cgcccggatc gcggcatcaa cataccgggc ccgtccggcg gcaccatggg
    10321 tatatgtgta aagctcgagc cggcttaccg tggggtcaac ggccgtcgtc ggccgcgacg
    10381 aataccgcga agatcccggc gtagggcttc accatgaagg cgggggcgcc gtagtagttg
    10441 ccgtcggcgt agatctgcga gagcgtcccg tccaggtaca acgcgtcgcg gcagccgagc
    10501 ttgttccgga acagcgatgc gaaggtgtgg aaattcaccg ggccggcgct gaccacgaac
    10561 acggcctggt cgggttgcgg cgcgcacacg ccgctgcgcc acttcatgct gtccgaatcc
    10621 gccacgaatt ccggattgat gttcccgccg atcaccaaca tcggcccgga ctgggtggcg
    10681 atgcggggtt cggtgccgga tttggcgaag gcctgggtgg tttgcaccga cgcatggccg
    10741 gcggcgtcga tcgcgaacac gccgttcggg agcagcgaga agttgcccgc ggcgggatcg
    10801 ccgtgggccc ggttgagcgg cgtcacggtc ctgccgtttt cgacgtggag cccgaggggt
    10861 tcgccgtgcg agtcgtagat gccggcattg gcggcgaaca gcagccgctg gccgttgcgg
    10921 gcgccccaca gttgcagggc gtggatgctg gcgaagggtt tgccggtggc ggggtcgcgc
    10981 cagtgcaggc cgagcttcgc ccgggcgagg tcgatcgtca ccacccggaa gtggacgttg
    11041 tcgaacgtga cgtcgtggct ctcgatgcca tgcgaaccgc gcgcgcaggc ggcgggcagt
    11101 agcacgatgg ccgcgaccag ccacgatcgg cgagccagcg cggcgcaatg tcggagggag
    11161 gatcggatcg gaagcatgga gggatgttcg cggcaccgcg gccgaggctg caacccgcgg
    11221 gcgcggcccg cgttcaggat cctcgccgac gcgcctcagc ggcccggctc gagcgtcagg
    11281 tgccaggctg caggccccgg cagcagcgga gtgggcttgc cgtgcaccca ggcctcgtgg
    11341 cccttgccga accacggcga gagcgggttg tcggactggc cggcaggcat ttcgaggata
    11401 ccgtgtgctt cgttgccggg ctgcacgtcc agccgcatgg atgcgccgaa ggaaggatgc
    11461 agcacgcgcg gcatgtcgtt gtcgccgggc agcgggtcgt gcggcatgtc ggcgaatcgc
    11521 gccagccagc cgggcaggac gaccgacaac gggttgtcga tccgtgccgc gttgcgccgg
    11581 ccccaggtct tgtccgcgag cctgccgggc tcctcggcca aggcgtccgc gacctgtcgg
    11641 gccgaagcct cgagcagagc atgccagtcc tggtattgcg gatcgagcag ccaggatggc
    11701 cgctgcgtca ccagcgtcca cgccgcgtat tcgcccaccg ccgaggtcgg ccactcgaaa
    11761 tccttgaact tcagcgaggc tcgagcggcg aacggcgcca acacgttttc gcgcacctgg
    11821 tcgtggaaca tgcgcacgat gcggtagccc acgctgtcgg ccgtggcgcg cgcctgccaa
    11881 ttgcgcacga agggtgcgag cgcgcgtgtg cccgggtcat ccgcgccgga aaggacgtcg
    11941 agtagcagct tctgccagcg cgcgaggaac agcgcccggt cgtcgagctg gatcgccagc
    12001 atgtcgcgcg gaatgaactg gtcatgggca gcgaggtcgt cgcggatctg ctgcgcgcgc
    12061 gcgcccgggt cgtagccgcc gtcgccgagc agttccagcg catcatccga aacgatccgc
    12121 tggttggcgg tccagatgcg atggtcggcc gggtcctcga cgcgcggata ttgcgcgtgc
    12181 gacgcgaacc cgctccagcc ggtgccgggt ttcgaccagt ccgcgggcag cgagggatcg
    12241 aacccggtgc gacgcgggat tgcgctgccg gcgatggtcc acccgatgtg gccctgcgcg
    12301 tcggtgacca cgaggttctg cggtgggatg cccatgccgg gcgcgagcga cagtgcctcg
    12361 tccaccgagg tcgcggtctc gagtttcatc aggtcgagat tgaccgcctg cggcgtttgc
    12421 gcgatccacg cgagtgcgag cggcgtgccg tccggcgcct cggccatcat cggtccccag
    12481 atggtgtcct ccaccgtgaa gtgcacgtcg gcggcaccct tcacgtggat gacctcgtcg
    12541 tgtctggtga tcgtcgccca gccatccggc gtcttgtagc gcgatgggtc ggcgggatct
    12601 cggatcacgc gcacccagtc catccaatca cccccgctgt tggtgaagcc ccaggcgacg
    12661 tggccgttgc tgccgatcac catcgcgggc gcgccgggca gggtgacgcc attcaagtcg
    12721 atcatccgcg agggatcggc ggggtcgggg tagcgcatgc gcgcgcggta ccagatgttc
    12781 ggcaccctca ggtgcaggtg cgggtcgttg gcgaggagcg ctgcgccacc ggcgagcgcg
    12841 ccgccgaccg cgaacgcgtt gctgccgacg ccggtgtttg cggccccggc gcgcccgacg
    12901 gcacgcgttc cccgggtcaa cgcgaccttg ttcgttgcgt gcgtgcgcag gtcgaacacg
    12961 gatgcggcgg gaaccgggac cgccgctgaa gcctgcccct gcaccggcgc ttcccagcgc
    13021 ggcgcgggcg ccagcaggaa gtcggcgagc gcatcgggca gttccgcgcg caggcgggcg
    13081 atgttcagct cgcgcgcgtt gtcgccgttc tggttgaggt cgaaatacat cgcgtcgatc
    13141 gtgagcacgg tgtcgccgtc ggtccacggc tgcggcctgg cccgcagcag caggtactcc
    13201 catgggcgca ccgacaggtg cgcgagtccg gcgttgacgc ccgcggtata ggcgtggacc
    13261 agcttgcgct gctccggcgc aaggtgcgcg agctgctgcc tggcgagtgc ccggaaacgg
    13321 tgctggcgat ggtcttcgtc ggacgcgacg gcagccttgc cgaccagcgc cgccagctcg
    13381 ccggcggcgc tgcggcgctg caggtccatc tggaaaaacc gctcctgccc gtgggcgaag
    13441 ccgagcgccc aggcgaggtc attgcggtcc cgcgcggtaa tcgtgggaac gccgtcgacg
    13501 tcgcgatcga tcgtcaccgg cgccgcgagg ccctgcacgc ggagcgtacc gtccgcggcc
    13561 gggcgactcc ccgcgagcag ccgccagccg aacgcaaacg tgatcaccag gacgatgatc
    13621 gctgcaacga ttacgcgtcg gacccagcgc atggactctc cccggacagt cgccgcattg
    13681 tgccttctga ttcgcgccat gtgtcaggca cgcgcctatt tttgctgaca tacggcgctt
    13741 atcctccccc ttccgcagga atggggaccg agggggatgg cttttcgtcg ccggcgtaca
    13801 tccccccgcc gctgcgcggc gccccccttc ctttggaagg gggtaaaagc gcatctctcc
    13861 gccgtttcca gtccccgctc cccggtccga cgctatcagt ttgtggcttt ttgcagtagg
    13921 gcgcgcattg cccgccctac tgcacgatac cggtccaggc tgcgctggcg aacttggctt
    13981 cggccagcgc ctcggcgcgt tccagttcct gcggcgacag ccggtcgcgc ctgaggccgt
    14041 gcgcggcgcg gaacgtgtcg atcatccgct cgatgatctc ctcgcgcggc aggccggtct
    14101 ggctgcgcaa gggatcgacg cgcttggcgg cgctcgcggt gcccttgtcg gacagctttt
    14161 cgcgcccgat ccgcaggacc tccagcatct tcgccgcatc gatgtcgtag gccatggtga
    14221 catggtgcag caccgcgccg ccccgacggg tctgcgcggc gcccgcgatc ttgccggacg
    14281 gcgaggtgat gtcgttgaga ggctggtacc aagcgtcgat gcccaggccg tgcaaggcat
    14341 cgatgaccca gccatccatg aactcgtagg actcctggaa gctcatgccc gcgaccagcg
    14401 cttccggcgc gtacagcgaa taggtaatgg tgttgccggg ctcgatgaac atcgcgccgc
    14461 cgccactgat gcggcgcacc acctcgattc cgtgccgccg cgcgccatcg ggatcgatct
    14521 cgttgcgaag cgattggaac cgtccgatca ccacgcacga cgacgaccac tcccacaccc
    14581 ggagggtcgg cggccggcgg cgtgcggcga cctcgtccgt cagcacctcg tcgatcgcca
    14641 tgtgcagcgc gggcggttgc ggcgtgccat ggaccaggcg ccattcgtag tcgttccagt
    14701 cggtgcggct cacgatgcgg cctcgagcgc gcgctgcacc gcgaccgcga tcgactcggg
    14761 cgagacgccg tacatcccga catgcggccc cagcgtcacg cgcacggccg cggcgaggtc
    14821 ttcaccgctg gagatggccg gcaacccttc cagcgcgaca tcgatcaggg tcagcgcggt
    14881 atccggctcc aggaaaaagt cgccgctgat ccgcacccgg aagagcttgc cgtcgcgcac
    14941 ctcgaggtcg gcgaccacca gcttgccgcc gggcatcttg tattccccat gcatcgcacg
    15001 attgtgccgc ttcccgcgac gcgcgtacag acgagccgac gcgaccggcc atggcaagca
    15061 ccgcgcgcaa gcgcatacta cgctccggcg gtgcgggatc ggaggtgggc atggagaact
    15121 tccgtgactt gtcggcgctc tgcggcgtgc tccggctgat gctcgaggag tcgccgcgcg
    15181 aagagcgcca ggccgcgttt gccgacgccc ttcaaaaggc ccttggattc gcgagcgaac
    15241 ccgccgggtt gtatgccgct acggtgttcc acgagaacac cggcgagtcg ccggaccggg
    15301 ttctagccaa cggatcgtgg ttgacgcgca cctgggtgag gggcgagcag cagggcaacg
    15361 tcggcgcatg gctcagcacg acgacggaac gatggcgctt cgatgaagac ctcacctacc
    15421 ggtacacgtt cgagcggatc gatacgggcc tgtcgagcgg tccgttcttc cagtcttcct
    15481 ataccaaacc aaggacgaac gaacagcgcg gcatctgggc ccctccggac acccagcagg
    15541 accagttcga attgttcgtg atgccattcg gcggcgtggc ccgcccgctg ccgttcgaat
    15601 gggtcgaaaa ggaggtgtac ggctatgcgg cgtgcatgat cggcggaacg agattcaccc
    15661 gcgaatagcc acgcccggac gtgctgcggc cgtttcgtat cgcgttggtc ccggacgccg
    15721 tgactcaagc cgattcgatg cgatcgaccc ggcgcagtcc gcgcggcagc aagccaccgc
    15781 gctgtgcgcg gttgccgccg tactcgacga ggtcggccca tttcagggtg agcttgcgct
    15841 ggccggcgta caaggtgact tcgcccgatc cctcggcgac caccgcgatg cccgcgagac
    15901 gctcctcgcc ggattgcagc ttcgccttgg ggatttcgat cagcttgttg ccacggccac
    15961 gatcgagctc gggaagctcc gccaccgaga acatcagcag gtggccggcg gtcgtcgctg
    16021 cgacgatccg gtcgcgggcg ggatcggcca cgccggcggg cgcgagtacg caagcgccgg
    16081 tatccatgtt gatgacctgc ttgccggcct tcttgttggc gagcagcgcc tcgaagcggg
    16141 tcaggaatcc atagccgctg tcggtggcga ggatcaggcg gtcggcgctg tcgccgatgg
    16201 cgagggcgtc gaagcgcgcg ccgggcgatg gcgtgaagcg accggtcagc ggctcgccgt
    16261 tgccgcgtgc ggagggcagg gtatgtgcgg gcgtggaata gcttcgcccg gtcgagtcga
    16321 tgaacgcggc ctgctgcgaa ctcttgccgc gcatgctggc gaggaacgcg tcgccctcac
    16381 ggtaggacag cgccgccgca tccacgtcgt ggccctttgc cgcgcgcgcc cagcccttct
    16441 tcgacagcac caccgtgaca ggctcggcgg tgacgagctg gctttcgtcg agcgcctgcg
    16501 cggcggcgcg ctccaccagt ggcgaacggc gcgcatcgcc gaatttgtcc gcatcggcct
    16561 tgagttcctg cttgatcaac gtccggaggc gcgccttcga cttcagggtc acgtcgatct
    16621 tcgcgcgttc ctcttccagc tcgtcgcgtt cggtgttgag cttcatttcc tcgagccgcg
    16681 cgagttgccg cagccgcgtt tcgaggatgt agtcggcctg ttcttcggaa agcttgaagc
    16741 gcttgatcag cgcgggcttc ggctcgtcct cgtggcggac gatccggatc acctcgtcga
    16801 ggttcaggaa cgcgatccgc agcccctcca gcaggtgcag gcggcgctcg accttctcga
    16861 gccggtgctg caggcgccgc gtcaccgtat cggtgcggaa gcgcagccat tcgtcgagga
    16921 tctgcttcag cgtcttgacc tgcggacgcc catccagccc gatcatgttg aggttgacgc
    16981 ggaaactctt ctcgagatcg gttgtggcga aaaggtgttg catcatcgcg tcggtatcga
    17041 cgcggttgga gcgcgggatc agcaccagcc gcacggggtt ttcgtggtcg gactcgtcgc
    17101 gcaggtcctc gagcatgggc agcttcttcg cgcgcatctg ggcggcgatc tgctcgacca
    17161 ccttggccgg cgatacctgg tggggcagtg cggtcagcac cacgttgccg cgctcctcgg
    17221 tgtacaccgc gcggcagcgc accgagccgg tgccggtcgc gtacatcgcc gccagctcgt
    17281 tgcgtggcgt gatgatctcg gccgcggtgg ggaaatccgg tccccggatg tggccgcaga
    17341 ggtcggcgat gctcgccgac ggctcgtcga gcaggtggat gcaggccgac acgacctcgc
    17401 gcaggttgtg cggcgggatg tcggtcgcca tgccgaccgc gatgccggtg ccgccattca
    17461 acagcacgtg cggcacgcgc gccggcagcc aagacggttc cttgagggtg ccatcgaagt
    17521 tgggcgtcca gtccacggtg cccatcgcca gttcgccgag cagcagttcg gcgatggggg
    17581 tgaggcgcga ttcggtgtag cgcatcgccg cgaacgattt cgggtcgtcc ggcgaaccga
    17641 agttgccctg gccatcgatc agcggataac ggtacgagaa cggctgggcc atcagcacca
    17701 tcgcctcgta gcacgccgag tcgccgtgcg ggtggaactt gccgatcaca tcgccgacgg
    17761 tgcgcgcgga cttcttgggc ttggcggtgg ctgcgagccc gagttcgctc atcgaataca
    17821 cgatgcgcct ctgcaccggc ttcaggccgt cgccgatgaa cggcagcgcg cggtcgagca
    17881 ccacgtacat cgagtagtcg aggtaggcgc gctcggcgta ttcgcgcagc gggatctgtt
    17941 cgaagttgga ttgcaatgcg gtcatggact ccccgagccc cggtgcgagc cgggagcagg
    18001 ctttcgaatc ggggaattgt cgcgggaagc cagcctcaat gcacgcgttg ggttgcgatg
    18061 ccgagtttcg cgagctgctt ttgcacgctg tcggggcccg cgaggtggcc ggcgccgacg
    18121 gcgacgaaca cggtgccgtg ctgctccagc aggccggcga gccggcccgc ccaggcgcgg
    18181 ttgcggtcca ccagcagcgc cttgtacagc tcgggatagt gctcgtgcat gccgccgttg
    18241 accaccgtgg cgatggcggc cacgttgccg gcttgccagt cgcggatcaa cgcctcgatc
    18301 tgcgcggggc ccttggcgta atcgtcgagc gcgttgcgca gcaggtcgag ttgcagggcc
    18361 ggggacaggt tcgccaggag cttgatctgc gcctcggcgg tttcgaacgc gccgaccgat
    18421 ttgcctgccg ccgtgaaggc atgatccagt tgcttgtcgg cgccggactt cgggtcgtac
    18481 ccggccttga cgatgggggc gacggcgatc gtcagcgccg ccagccaggg cttcatcgcg
    18541 ttcaaggtgg ccgcgccgcc tgcgacgccg atggcttcgg cggccgccgc gagcctcgcc
    18601 tggtcggtgg catccagctt gtccgatagc ggatgctgga ggtccatgcc gtactcgagc
    18661 acgagcatct gcatgtcgag cggcgagtcg tcgtcttcct cgacatacag cttttcgctt
    18721 gccgccagcg cctcgtccag cgccgggtag tgccaatgcg cacccttggg caacacgtgc
    18781 accgtcccga acaggtagac ggtcgcggag gggcctttcg cgacccacag cgccgggtcg
    18841 gccagcgcgg gcgtgctggc gatcagcacg aatgcgaggg cgaagcagaa acgtcgaagc
    18901 gttttcatcc gggaacgagg cgcaaagaca ccgcgcattg tgtcatggac gtgatgggca
    18961 ggcgggctgc cgcgccggaa cgagggttgt tccggcgtcg gttccgcgag gcggattcaa
    19021 ggtgcctgcg cctgcctcct cgatgcttgc actgcctcga ggacgatttg ctgcgcgcgc
    19081 gccgagcgtt gcggcgacaa gaggacctga tgccaggcgg ccttcgcttc cggtgtcttg
    19141 cccagacgcg ccgcgaggcg cgcccgcagc gcctgggctc gcaggaaatc gggcagggcg
    19201 gcagtggcgt gctgcatcgt ccgcagcgcg ccttcggcgt cgtgttccag tagcagggcg
    19261 cgcgcccgcg ccagccagct ctcccggctt tgcgaccagg catcggcacg atccaatacc
    19321 cgcaacgccg ccgccggctg ccggtcggcg atcagcagcg acgccagggc aacgccgctt
    19381 tcgtgatccc acggcgcgcg cgccagtgcc ttttcgtaaa gacgtaccgc cacgcggggt
    19441 ggcgcgattg gtgccatcgc ggcggccgtc gccgcttccc ggttttcggt cagcagcgcg
    19501 aaagcgcgat ggctggacca tcccgtgacg gcgagcatcg cgcagactgc ggcggcgccg
    19561 ccggcgcgtt gccatgcgcg gcggccggca tgcgcccggc acaatgcacc ggctggcgcc
    19621 gcctccgaca accccgcaac gcagccgagc acgagccaga acagcagcgc cgtcgcaggc
    19681 gtgtgcaacg gcgccgacgc ccaggcgagc atgaagagta cccccagcga agcggcaagc
    19741 gcgggcacgg ccgtggcgac gcgggtgcgg tttggcgtgc acggttcgtc cggtgacttc
    19801 gtgagcggga gtggcggaat cgaatcgagg gcacgtcgtg cggcgtgcaa ccccagtgcg
    19861 cacgacgcga gtagcagcaa cccgccaggg aggccggtct ccaccgccgt ctcgaggaag
    19921 tcgttgtgcg catgcgcggt cagcaggtcc ggcatccggc tgcgtgcgac cgcgtccagc
    19981 ggcgccgcga cggcacggag cccgtcggga agtaccaacg cgaagtgatc gaggccgtag
    20041 ccatggacag gccgcgcggc gatcaggtgc agcgacgcct gccacagatg cacgcgcgag
    20101 agcacggaat gccaatcgtt ccaccgcagc agtgccgccg cgtagccgtc atcgcgcaac
    20161 agaagggcga gcgatcccag cgcgaccacg gcggtcagga aaaaacctgc ctggtttcgc
    20221 gggatgcggc tgtcgccgtg gccgcgctgg ctcgtccacg cgtaaatggc ggcgccgaca
    20281 agcagactcg ccaagcccag ccgcgagccg gtgggcagga tggccatcgc gaacagcagc
    20341 cagagccacg cggtcccgcg cgtgtagcgg gacgcccacc acacggcaat gggggcgctg
    20401 gacgccagga attctgcggt ccag
//
LOCUS       DASSNH010000124         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_12680, whole genome shotgun sequence.
ACCESSION   DASSNH010000124 DASSNH010000000
VERSION     DASSNH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12680"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFK00_04450"
     CDS             complement(<1..225)
                     /locus_tag="VFK00_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HET7592239.1"
                     /translation="MNKKIYSLVWNKSLRQIVVASELASVKSGGGSTGETSGARRGLR
                     GAALCAALALCLAPWSAALAGQTDCSGATAP"
     gene            complement(624..1661)
                     /locus_tag="VFK00_04455"
     CDS             complement(624..1661)
                     /locus_tag="VFK00_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7592240.1"
                     /translation="MGNGTSKREAPARPLELLGISDIEERAYRALLTRRMATAVEIAR
                     QIRITPRAARRLLADLESLGLATHTPRIPRVYVASPPEFTVTALIKQRQAELERARTI
                     IPDLEEQSAQADNGNSHGPVLELVSNRAQLGVVLQQMYKSFRTEAMCFQRAPTLAPGA
                     VPPRRLRAGMQVRTISDSSMFELPGMMGRLKDDVGRGEQARMIETLPFKMMIFDRRAA
                     VLTLDSESPEKSPTLLVHGGALLKALCLLFEFMWERATPIVFGHGDRLKSQQGDARLQ
                     EVAESLVPLLAAGLNDKAIAAELGISSATLNRRIADLMKMYGTRSRFQLGWRAALDAS
                     APAPTGALRRK"
     gene            complement(1963..2036)
                     /locus_tag="VFK00_04460"
     tRNA            complement(1963..2036)
                     /locus_tag="VFK00_04460"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2002..2004),aa:Cys,seq:gca)
     gene            complement(2151..2226)
                     /locus_tag="VFK00_04465"
     tRNA            complement(2151..2226)
                     /locus_tag="VFK00_04465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2191..2193),aa:Gly,seq:gcc)
     gene            complement(2263..2880)
                     /gene="pgsA"
                     /locus_tag="VFK00_04470"
     CDS             complement(2263..2880)
                     /gene="pgsA"
                     /locus_tag="VFK00_04470"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7592241.1"
                     /translation="MRVNLPTWLTLFRVLLLPVMVVVFYSHDAIPAIPLRAANVAAVV
                     VFALAAVTDWLDGWIARRFHMESAFGAFLDPVADKLMIAVTLFVLVQSHPTALLAVTS
                     AVIVGREITVSALREWMAFVGERKRVKVQWIGKFKTVMQIVAILILLWERDKDAILLR
                     VWYVGEVLLVVAAILTIWSGLAYMKAAWPVLKEGRMPGERDEPQA"
     gene            complement(2975..>3635)
                     /locus_tag="VFK00_04475"
     CDS             complement(2975..>3635)
                     /locus_tag="VFK00_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7592242.1"
                     /translation="DEAPMRLECFDISHTMGEETVASCVVFGPEGPLKNHYRRFNIRG
                     ITGGDDYAAMRQALERRFKHLARGEGEAPDVLLIDGGRGQVGEALAVLRGLGLDGIRV
                     VGVAKGPSRRAGEETLVLGDSGRELEPGPDSPALHLIDAVRDEAHRFAITGHRRRREK
                     ARERGVLEDVPGIGAARRSALLKAFGGHAGVVGAGVEELCRVRGINRELAERIHAYLH
                     G"
BASE COUNT          625 a         1274 c         1165 g          571 t
ORIGIN      
        1 cggcgcggtc gcaccactgc agtcggtctg gcccgccagt gccgccgacc agggcgccag
       61 gcacaacgcc agcgccgcgc acaacgccgc accccgcaag ccacgccgcg caccgctggt
      121 ttcgccggtc gatccacctc cgctcttgac cgacgcaagt tcggacgcca ccacgatctg
      181 gcgaagggac ttgttccaaa ccaggctgta gatcttcttg ttcatcttct ggcctcagcg
      241 gtaagttgtt ggacggcgtg cgaaatagct tggcgcctgg tgctggcgtt tcctcgtgca
      301 gcgtatggct atgggccgtc gctggaagcg acggaaggga tgtcgacgtg ggctacaggc
      361 aacgcgacgc gccgcgcgca gcagttgtgc cggcacggtt ccggatcaaa ttggctctga
      421 tcgatcgcgg cgacccgcga gatgcgtcca tcgcccctgt actccccaaa cgactccgtt
      481 cgaccagtct ggtacgggcc cggatcgagc gcaaggcttc gccgcgatgg ataaccatca
      541 tgatagaaag ccatcattgc gcggctgggc cagcctccag gagagccatt cgcaccgtcg
      601 gccagaacgg gcttgcgctc gggctatttg cggcgcaatg cgccggtggg cgccggtgcc
      661 gacgcgtcca gcgccgcccg ccatccgagc tggaagcgac tccgggtccc atacatcttc
      721 atcaagtcgg cgatgcgacg attgagggtc gccgacgaga tgccgagttc cgccgcaatg
      781 gccttgtcgt tgaggcccgc cgcgagcaac ggcaccaggg actccgccac ttcctgcaac
      841 cgcgcatccc cctgctgcga cttcaaccga tcgccatggc cgaacacgat cggggtggcg
      901 cgctcccaca tgaattcgaa cagcaggcac agcgccttca gcaaggcccc tccgtggacc
      961 agcaacgtag gcgatttttc cgggctttcg ctatccagcg tgaggacggc ggcgcggcga
     1021 tcgaagatca tcatcttgaa cggcagcgtt tcgatcatgc gcgcctgttc gccgcggccc
     1081 acgtcgtcct tcaggcgacc catcatgccg ggcagctcga acatgctgct gtccgagatc
     1141 gtcctgacct gcatgccggc gcgcagccgg cggggcggaa cggcgcccgg ggcgagggtg
     1201 ggtgcgcgct ggaagcacat cgcctcggtg cggaacgact tgtacatctg ctggaggacc
     1261 acgccgagct gggcacgatt gctgaccagc tccagcacgg gcccgtggct gttgccattg
     1321 tcggcctgcg cggattgctc ctcgaggtcc gggattatgg tccgggcccg ctccagttcc
     1381 gcctgccgct gcttgatgag cgcggtgacg gtgaattcgg gaggcgatgc gacatagacc
     1441 ctggggatcc gcggggtgtg cgtcgccagg cccagcgact cgaggtccgc gagcagccgg
     1501 cgggcggcac gcggcgtgat gcggatctgt ctcgcgatct cgaccgcggt cgccatccgg
     1561 cgcgtcagca gcgcgcggta ggcgcgctcc tcgatgtcac tgatgcccaa caattcgagc
     1621 ggccgggcag gcgcttcgcg tttgctcgtt ccgttcccca tgcgtgcctc cctgagtccc
     1681 ctcgaaagcg ccggcgcgca acaccagcgc gtggcctaca aggtaaccgg aggtcggcat
     1741 ccggaaaagg cgccaccgaa atgcccaccg gtcgggcgtt gacgtacggg ctcgtcgccg
     1801 cctgaagcgg gaggcgcccg ccctggatcg ggatcccggg gcgcgcgggg cagccggtgg
     1861 gagcaaggcg tggtacgggg tgggccatca agcccggcgc ccgatccacg cctgatcgcc
     1921 gacagaaacg aggaggagcc ctcgtcccgc atctccgaaa catggaggcc agggtcggaa
     1981 tcgaaccggc gtacgcggat ttgcagtccg ctgcatgacc actctgccac ctggccggtg
     2041 ggtgaaagcc cgtccgcgat tccagggttc gcggcgcctg gaccttcggc agggccgcct
     2101 tgcgcggccc tcaaaaacga aaccccggcg cgccggggtt cgaaaaatcc tggagcggga
     2161 aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat
     2221 tcccgcgcga gccggcaatg atacggtggg ctgcaatccg gttcaagcct gcggttcatc
     2281 ccgctcgccg ggcatccgcc cctccttcag gaccggccat gcggccttca tgtacgcgag
     2341 ccccgaccag atcgtcagga tcgccgcgac caccagcagc acctcgccga cgtaccagac
     2401 ccgcagcagg atcgcgtcct tgtcgcgttc ccacaggagg atgaggatcg cgacgatctg
     2461 catcaccgtc ttgaacttgc cgatccactg caccttgacg cgcttgcgct cgccgacgaa
     2521 cgccatccat tcgcgcaacg cggaaaccgt gatctcgcga ccgacgatca cggcgctggt
     2581 gaccgcgagc aacgcggtcg ggtggctctg caccagcacg aacagggtca ccgcgatcat
     2641 cagcttgtcg gcaaccggat cgaggaacgc gccgaacgcc gattccatgt ggaaccgcct
     2701 cgcgatccag ccgtccagcc agtccgtgac cgccgccagc gcgaacacga ccacggccgc
     2761 cacgttggcc gcccgcagcg ggatcgccgg gatcgcgtcg tgggaataga acaccaccac
     2821 catcaccggc agcagcagta cacggaacag cgtcagccag gtgggaagat tgactcgcac
     2881 aggacgattc cggtcgttgg gtccgacagg gcaacccggc aagtctctca cacgttcgcg
     2941 ccgaccgccg ggagaccctt ttcagcaaac gcacctatcc gtgcaggtac gcatggatgc
     3001 gttcggccaa ctcgcggttg atgccgcgca cccggcaaag ctcctcgacg ccggcgccca
     3061 ccaccccggc atgtccgccg aatgccttca gcagggccga gcggcgcgcg gcgccgatgc
     3121 ccgggacgtc ctccagcaca ccgcgttcgc gggccttttc gcggcgcctg cggtgaccgg
     3181 tgatcgcaaa acgatgggcc tcgtcgcgca ccgcatcgat caggtgcaag gcaggggagt
     3241 cgggaccggg ttcgagttcg cggccgctgt cgccgagaac cagggtttct tcgcccgcgc
     3301 gacgcgacgg ccccttggcc acgccgacaa cgcggatgcc atcgaggccc aacccgcgca
     3361 acaccgccag cgcctcgccg acctggccgc gaccgccgtc gatcagcagc acgtcgggcg
     3421 cctcgccttc cccacgcgcg agatgcttga aacgccgttc cagcgcctgc cgcatggcgg
     3481 cgtaatcgtc gccgccggtg atgccgcgga tgttgaaacg ccgatagtgg tttttcagcg
     3541 gcccctccgg accgaacacc acgcacgacg ccaccgtttc ctcgcccatg gtgtggctga
     3601 tgtcgaagca ctcgagccgc atgggtgcct catcc
//
LOCUS       DASSNH010000125        10195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_250994, whole genome shotgun sequence.
ACCESSION   DASSNH010000125 DASSNH010000000
VERSION     DASSNH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250994"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /gene="dxs"
                     /locus_tag="VFK00_04480"
     CDS             <1..1674
                     /gene="dxs"
                     /locus_tag="VFK00_04480"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET7592243.1"
                     /translation="GGHFGAGLGVVELTIALHYLYDTPHDRLVWDVGHQGYPHKILTG
                     RRDAITSIKTRGGLAPFLRRAESEYDTFGVGHASTSISAALGMAIALRQRGDRRNVVA
                     VIGDGALTGGMAYEALNHAGGTGADLLVVLNDNGMSISENVGGLDRTLARLRAGKPGE
                     SGPHPEALFEELGFAYSGPVDGHDLPMLLRALREARQRHGPRLLHVITTKGKGYAPAE
                     LAQIDYHAVGRFDPARGVAQATAGKPTYSEVFGDWLCDMAAQDTRLVAITPAMREGSG
                     MVRFSRAFPDRYFDVAIAEQHAVTLAAGLACEGAKPVVAIYSTFLQRAYDQVIHDVAL
                     QGLDVTFAIDRAGVVGPDGPTHAGSFDLSYLRCIPNMVVMAPADDNECRQMLTTGFRV
                     DGPAAIRYPRGRGPGVAIEPGLEALPIGKAEVRRRGHRLALLAFGCAVAPADTIGAEL
                     DATVVNMRFVKPLDESLVLALARSHEAFVTLEDNVVAGGAGSAVGELLAAHGIEIPLL
                     QLGLPDAFLQHATREELLAEAGLDAAGIRHAIRARFPQHVSVKRCAGLD"
     gene            complement(1822..3936)
                     /locus_tag="VFK00_04485"
     CDS             complement(1822..3936)
                     /locus_tag="VFK00_04485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7592244.1"
                     /translation="MNLRLAACALALAVCSGAAMAAQHPFDAHDLAMMDRVSDPHLSP
                     SGNEVAFTVRSTDWDANKGVTSVWLLDLRQKAAQPRRVTDPKLGANSPAFSPDGRTLY
                     FLATEKDSTQLWKAPVSGGAPEQVTHLPLDVDNFKLSPDGKSLLFSAQMFEDCTTLEC
                     TRGKLDAQKNVKATGRLYHRLFVRHWDTWSDHRRGQLFVVPLDGSAKPVLLTKGIDGD
                     VPSKPDGSNAEYAFSPDGKTVYFDVRIAGKTEPWSTNFDVYSVPADGSAAPKNLTASN
                     QAWDAYPLPSHDGKTLYYLAMKTPGFEADRFGIMALDLASGTAREIDPDWDRSAGPLV
                     LSADGKTLYTTVADEGNVALYAIDVANGRPTRLLSDGSVLGFDVAGDRIVAARQDFSH
                     PTDLYTLGKQGGHPTQVTHFNAARLADIEFSKATWFTFKGWNGDTVHGYVMPPVGAKP
                     GQKYPVAFLVHGGPQGAWPDEFHYRWNPQVYAGAGFGVVAINFHGSVGYGQAFTDAIS
                     QHWGDRPLEDLQKGWAAALKQFPYLDGNDACALGASYGGYMIYWMAGVWNQPWKCLVD
                     HDGVFDARMMYYATEELWFEEHENGGKQFERPENYEKFNPLNHVKDWRVPMLVIHDSK
                     DYRIPVSQGLGAFTALQRQGIPSEFLNFPDENHLVLKPQNSVMWHKTVIDWLKRWTGP
                     DAPQAVRSGAKEAKPAESQSAQ"
     gene            4105..4941
                     /locus_tag="VFK00_04490"
     CDS             4105..4941
                     /locus_tag="VFK00_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7592245.1"
                     /translation="MAEHEAHPRPHVNYVTFPSAARGAPLTIPAQLRIPSGTDDCPAV
                     VIVHGTAGLDSRGAYHAEALNGAGIATLEVDMWSPRSLHGGVGTSGRPQTVVETLPDA
                     FGALLYLGSCPGIDPQRIGIMGFSWGGVVSMLTATRPYNERYLGAQPLRFAAHAPFYP
                     VCWTYNRVPGHEFMELTGAPVLIQTGECDDYDKPDTCDILVHSLPGEARSHVTVMTYP
                     GAAHAFDRLEPAQTVSDPHSHLGQGGEVIMAGDPAAADKSRRTTVDFFARAFAAVGEA
                     SR"
     gene            complement(5132..5207)
                     /locus_tag="VFK00_04495"
     tRNA            complement(5132..5207)
                     /locus_tag="VFK00_04495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5172..5174),aa:Thr,seq:cgt)
     gene            5357..6967
                     /locus_tag="VFK00_04500"
     CDS             5357..6967
                     /locus_tag="VFK00_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7592246.1"
                     /translation="MNKGTTGGAEHIRRELRVLDIYRVFQALVYTVLSLSSLVFMWMP
                     LRNPLLARVTALVYLLLALLAVARDLRGWRQLIGTTSVFLTIDVAAAFLAAVTIPAAH
                     TGIAAMLVINVGAAALLLSPRLGGLFAALATLAMIAQALLVPNSGDVGPALLEAGLFG
                     VAYFATAALGYALGSQMRASEALAEQRGSDLASLAQVNDLIIRRMKTGVLLVDESNRI
                     HQINESAWMLMGNPGVEQRDLGTVAPELSRRLYHWRTTHEHDASAIALAEGAPEIIPR
                     FTRLSAHDDSYVLIFLDDTSLLSQRAEQLTLTTLGRLSASIAHEIRNPLAAIRYSAQL
                     LAESEAIGDADQRMVEIINNHCGRVNEIIENILQLSRRERSRPESLDLNNWAARFVDE
                     YKEANEIGHDSLRPLLYPRPVEALADTQHLQQVVWNLVQNALRYGRMPDEPARVMVVA
                     RLASDNGPPLLEVIDRGPGIPPKVAAQIFEPFFTTHQYGTGLGLYLAKQMCEANQANL
                     EYVPVAGGGSCFRITLTAPLRTPMGSET"
     gene            7047..8426
                     /locus_tag="VFK00_04505"
     CDS             7047..8426
                     /locus_tag="VFK00_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7592247.1"
                     /translation="MTRQTVLVVDDERDIRELLSITLGRMDLDVDAAANVTDAKKMLS
                     EVRYDLCFTDMRLPDGTGQELIELIAREHPDTPVAMITAYGNVEAAVDALKAGAFDFV
                     SKPVDIHMLRRLVQTALRVGQAPETSDEEAPETRLVGESQALKEVRSRIAKVARSQAP
                     IYISGESGVGKELVARSIHELGPRSAGPFVPVNCGAIPSELMESEFFGHQKGSFTGAH
                     ADKEGLFQTANGGTLFLDEVAELPLHMQVKLLRVIQEKAVRPIGGRGEIPVDVRILSA
                     THKDLARLTQSGQFRQDLYYRINVIELRVPPLRERAGDIPVLATHILKKLAEQAGVPV
                     PAVQPAALTALQDYDFPGNVRELENVLERAIALCENGVIEPADLMLEPAARGTNEGDE
                     DETLEDDDDASGASGGAVGLDDYISSIERDAIVKALKETRYNKTAAAKNLGITFRALR
                     YKLKKLGID"
     gene            complement(8442..9350)
                     /locus_tag="VFK00_04510"
     CDS             complement(8442..9350)
                     /locus_tag="VFK00_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7592248.1"
                     /translation="MKDLPLNALRVFALACAHGGVRAAARELGIAHSSVSRHLAELEA
                     WLGVPLAREKGGGRRGWTPTPQGEALGKAVLAGLRDIGAATLAIREVRSAFAVTISAA
                     PSFAARWLLPRLPALERAHPRIELSVLVNQRTEDLRAGGIDLAVRMGAGPWPGLHCEP
                     LMDDALYPVMSPGLWKKAGRPSRPQALASLRLLHDRDPHAGWEQWRERFGPASLNLQR
                     GPRFASSDLLLRAAAQGQGVALARHRLAVDDVAAGLLVRPFGERMLRIENAYWIVRTD
                     RAPRHAVATVIAWLERQPGGGEAATG"
     gene            9438..10019
                     /locus_tag="VFK00_04515"
     CDS             9438..10019
                     /locus_tag="VFK00_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET7592249.1"
                     /translation="MTTLFSTGLFLLIAAITPGPNNLVVMRAAARNGWRAALPAAAGV
                     ITGGLVLLAVVTAGADGAFAAWPWLRGMIELAGVLYLVWLGTRMIAAAGEDAGSAALP
                     AGLPGLFAFQFLNPKGWVMVLTVVAALPGANGAHALLYLAPLFVCIPAACLLLWAGLG
                     GALAPYLGRPVVRQWTDRVLGALLILAALLLFT"
BASE COUNT         1710 a         3527 c         3310 g         1648 t
ORIGIN      
        1 ggcgggcact tcggcgccgg gctcggcgtg gtcgaactca ccatcgcgct gcattacctg
       61 tacgacaccc cgcacgaccg cctcgtgtgg gacgtcggcc accagggata tccgcacaag
      121 atcctcaccg gccgccgcga tgcgatcacc agcatcaaga cgcgcggcgg cctcgccccg
      181 ttcctgcggc gcgccgaaag cgaatacgac actttcggtg tcggccacgc atccacttcg
      241 atctcggctg cactgggcat ggcgatcgct ttgcgccagc gcggcgaccg acgcaacgtg
      301 gtcgccgtga tcggcgacgg cgcgctcacc ggcggcatgg cctatgaggc gttgaaccac
      361 gccggcggga ccggcgccga cctgctggtg gtcctgaacg acaatggcat gtcgatcagc
      421 gaaaacgtcg gcggactcga ccgcaccctc gcgcgactac gtgccggcaa gcccggcgaa
      481 tccggtccgc accccgaggc gctgttcgag gaactcggct tcgcgtactc ggggcccgtg
      541 gatggccacg acctgcccat gctgctgcgt gcgctccgcg aggccaggca gcggcacggc
      601 ccgcggctgc tgcacgtgat caccaccaag ggcaagggct acgcgcccgc cgaactcgcg
      661 cagatcgact accacgccgt gggacgattc gatcctgcgc gaggggttgc gcaagccact
      721 gcgggcaagc cgacctacag cgaagtgttc ggcgactggc tgtgcgacat ggccgcgcaa
      781 gatacgcgcc tcgtcgcaat cacgcctgcg atgcgcgaag gctccggcat ggtgcggttc
      841 tcgcgcgcat tcccggaccg gtacttcgat gtcgcgatcg ccgaacaaca cgccgtcacg
      901 cttgccgcag gccttgcctg cgaaggcgcc aaacctgttg tcgcgatcta ctcgaccttc
      961 ctccagcgcg cctacgacca agtgatccac gacgtcgcgt tgcaaggcct cgatgtcacc
     1021 ttcgcgatcg accgcgccgg cgtggtcgga cccgacggcc ccacgcacgc cggcagcttc
     1081 gacctctcgt acttgcgctg catcccgaac atggtggtga tggcaccagc cgacgataac
     1141 gagtgccgac agatgctgac gaccggcttt cgtgtcgacg ggcctgccgc gatccgttac
     1201 ccgcgcggcc gcgggcccgg tgtcgccata gaacctggcc tggaggcatt gccgatcggc
     1261 aaagccgagg tgcggcgtcg tggccaccgc ctcgcactcc tggcgttcgg ttgcgccgtc
     1321 gcaccggccg acaccatcgg cgccgaactc gacgccaccg tcgtcaacat gcgtttcgtc
     1381 aaaccgctcg acgaatcgct ggtgctggca cttgctcgca gccatgaggc attcgtcacg
     1441 ctggaagaca acgtggtcgc cggcggcgcc ggttcggccg tcggcgaatt gctcgccgcc
     1501 cacggcatcg aaatcccgct gctgcagctc ggactgcccg atgcgttcct gcaacacgcc
     1561 acgcgcgaag aactgctcgc cgaggccggc ctcgacgccg ccggcatccg gcacgcgatc
     1621 cgagctcgct ttccgcagca tgtatcggtg aagcgatgcg caggactgga ttagaaagta
     1681 ggttgggctg acgccgcttt ttggcggaag cccaacgacg cgacaccgtt gggctccgct
     1741 gcgctccggc caacccacat gcccggccgc gtgccggcat tcactcgata gaagccggcg
     1801 ccgactgact ggaggcgagg ttcactgcgc gctttgcgat tccgcaggct tcgcctcttt
     1861 tgcgccggac cgcacggctt gtggtgcatc cggtcccgtc cagcgcttca gccaatcgat
     1921 cacggtcttg tgccacatca cgctgttctg cggcttcagc accaggtggt tctcgtccgg
     1981 gaagttcagg aattcgctgg gaatgccctg ccgctgcagg gcggtgaagg cgccaagccc
     2041 ctgcgatacc gggatgcggt aatccttgct gtcgtggatc accagcatcg gcacgcgcca
     2101 gtccttgacg tggttcagcg gattgaactt ttcgtaattc tccgggcgtt cgaattgctt
     2161 gccgccgttt tcgtgttcct cgaaccacaa ctcttcggtg gcgtaataca tcatgcgcgc
     2221 gtcgaacacg ccatcgtggt cgaccaggca cttccacggt tggttccaga cgcccgccat
     2281 ccagtagatc atgtagccgc cgtagctcgc gcccagcgcg caggcatcgt tgccgtcgag
     2341 gtacgggaac tgcttcagcg ccgccgccca acccttctgc agatcctcga gcggacgatc
     2401 gccccagtgc tgcgaaatcg catcggtgaa cgcctgtccg taaccaaccg atccgtggaa
     2461 gttgatcgcg acgaccccga atccggcgcc ggcatacacc tgcggattcc agcggtagtg
     2521 gaattcgtcc ggccacgcgc cctgcgggcc gccgtggacc aggaacgcga ccggatattt
     2581 ctggcccggt ttcgcaccca ccggtggcat cacgtagccg tgcaccgtgt cgccgttcca
     2641 gcccttgaag gtgaaccagg tcgccttgga gaactcgatg tcggcgagac gcgcggcgtt
     2701 gaaatgggtg acctgcgtgg gatggccgcc ttgtttgccc agcgtgtaga gatcggtcgg
     2761 gtggctgaag tcctggcgcg cggcgacgat cctgtcgccg gcgacatcga agccaagcac
     2821 gctgccgtcg gataagagcc tggtcggcct gccgttggcg acgtcgatcg cgtacagcgc
     2881 gacgttgccc tcgtcggcaa ccgtggtgta gagcgtcttg ccgtctgccg aaagcaccag
     2941 ggggcccgcc gaacgatccc agtcgggatc gatctcacgc gcggtgccgc tcgcgagatc
     3001 cagcgccatg atgccgaagc gatccgcctc gaaaccgggc gtcttcatcg cgaggtaata
     3061 aagcgtcttg ccgtcgtgcg agggcagcgg gtaggcgtcc cacgcctggt tcgaagccgt
     3121 caggttcttc ggcgccgccg aaccgtcggc cggcaccgaa tacacgtcga aattggtcga
     3181 ccacggctcg gtcttgccgg cgatccgcac gtcgaaatac accgtcttgc catcgggcga
     3241 gaacgcgtac tcggcattgc tgccatccgg cttcgacggc acgtcgccat cgatgccctt
     3301 ggtcagcagc acgggcttgg ccgagccatc cagcggcacc acgaacaact gcccgcggcg
     3361 atggtcggac caggtgtccc aatgacgcac gaacaaccgg tgatacagcc gcccggtcgc
     3421 cttgacgttc ttctgcgcgt cgagcttccc gcgcgtgcat tcgagcgtcg tgcagtcctc
     3481 gaacatctgc gccgagaaca gcagcgactt gccatccggt gagagcttga agttgtcgac
     3541 atcgagcggg aggtgcgtca cctgctccgg cgccccaccc gaaaccggcg ccttccacaa
     3601 ttgcgtcgaa tccttctccg tcgcgaggaa gtacagggtc ctgccgtccg gcgagaacgc
     3661 ggggctgttg gcaccgagct tcgggtcggt gacgcggcgc ggctgcgcgg ccttctgacg
     3721 cagatcgagc aaccagacgc tggtgacgcc cttgttggca tcccaatcgg tgctgcggac
     3781 cgtgaacgcc acctcgttgc cgctgggcga aagatgcgga tccgacaccc ggtccatcat
     3841 cgccaggtcg tgcgcatcga aggggtgttg agcggccatc gcggctcccg agcaaacggc
     3901 cagcgcgagc gcgcaggcgg caaggcgaag gttcatcgtc gcgtcccggc atccgtggaa
     3961 tcgcaacgtt agcacgtgcg cgcgcacgcc cgccacgccg gcggtcgaac cgcgtcctca
     4021 tccagcctcc gctatttgcg agtgcgacag gtgtcaacga gaccgtcttg tcgctaaagt
     4081 cgacgccgaa tccagggagt cgacatggcc gagcacgaag cacatccacg accgcacgtg
     4141 aactacgtga ccttccccag cgccgcgcgc ggagcgccgc tgacgatacc cgcccaactg
     4201 cgcatcccct cgggcactga cgactgcccg gccgtcgtaa tcgtgcacgg tacggccggg
     4261 ctggacagcc gcggcgctta ccacgccgag gcgctgaatg gcgccgggat cgccacgctg
     4321 gaagtggaca tgtggtcgcc acgcagcctg cacggcggcg tcggtaccag cggccggcca
     4381 caaaccgtcg tggagacgct cccggatgcc ttcggggcgc tgttgtacct cgggtcgtgc
     4441 ccaggtatcg atccgcagcg catcgggatc atgggatttt cctggggcgg cgtggtttcg
     4501 atgctcacgg ccacccgtcc gtacaacgag cggtacctcg gcgcccaacc actgcgcttc
     4561 gccgcacacg cgcccttcta ccccgtgtgc tggacctaca acagggtccc cggtcacgaa
     4621 ttcatggagc tcacgggagc gccggtcctt atccagactg gcgaatgcga cgactacgac
     4681 aagcccgata cgtgcgacat cctggtccac agcctgcccg gggaagcccg aagccatgtc
     4741 actgtcatga cctaccctgg agccgcccac gcattcgatc gcctggagcc ggcgcagacg
     4801 gtaagcgacc cccattcgca tctgggacag ggcggcgaag tgatcatggc gggcgatccg
     4861 gcggcggcgg acaaatcgcg acggacgaca gtcgacttct ttgcgcgagc ctttgcggcg
     4921 gtcggcgagg cgagccgctg atcgatctgc tttgccttcg atcgcctgag gggcacattc
     4981 gccggcccgc gtatcgcatc ctcattcggt gagcatgcgc cgccctgctt ttgcagatgg
     5041 ccccacgccg atcgaacgcg catcgatcga cgcgcgacat ccaggaatgc ggttcacccg
     5101 atatttcgcg aagtcgcctg gaacaggttt ttggtgccgg aagagggaat cgaacccccg
     5161 acccacgcat tacgaatgcg ttgctctacc agctgagcta ttccggcacg ggcggaccgg
     5221 ctagtgtacg ggtggctccg ggatggcgca acgcgcgggc ctggttggca gttcgcggat
     5281 gcacgggctt gcggcccttg tgcgaacctt cggcgcgaag catgatcgcc gggtggcgac
     5341 tacgacccag gccatcatga acaagggcac gaccggcggc gccgagcaca tccggcgcga
     5401 gttgcgcgtg ctcgacatct accgggtatt ccaggcgctg gtctacacgg tgctgtcgct
     5461 cagcagcctc gtcttcatgt ggatgccgct gcgcaatcca ctgctcgcgc gggtgacggc
     5521 gctggtgtac ctgctgttgg cgctgttggc cgtggcgcgc gacctgcgcg gctggcgcca
     5581 actgatcggc acgaccagcg tgtttctcac gatcgacgtg gccgcggcgt tccttgccgc
     5641 ggtgacgatt cccgccgcgc ataccggcat cgccgcgatg ctggtcatca atgtgggggc
     5701 ggccgcgctg ctgctgtcgc cccgcctggg cgggcttttc gcggcgctgg cgaccctggc
     5761 gatgatcgcg caggcgctgc tggtgccgaa cagcggcgac gtaggtcccg ccctgctcga
     5821 ggccggcttg ttcggggtcg cctacttcgc caccgccgcg ctgggttacg cgctgggcag
     5881 ccagatgcgc gccagcgaag cgctggccga gcagcgcggc agcgatcttg cgagcctcgc
     5941 gcaggtcaat gacctcatca tccggcgcat gaagaccggc gtgctgctgg tggacgaaag
     6001 caaccgcatc caccagatca acgaatcggc gtggatgctg atgggcaatc cgggggtcga
     6061 gcagcgcgac ctcggcacgg tggcgccgga actctcgcgc cggctgtacc actggcggac
     6121 cacccacgaa cacgacgcca gcgccatcgc gctggccgag ggtgcgccgg aaatcatccc
     6181 gcgcttcacg cgactctccg cgcacgacga ttcctacgtg ctgatcttcc tcgacgacac
     6241 ctcgctgctg tcgcagcgcg ccgaacagct cacgctgacc acgctcggcc ggctgtccgc
     6301 ctcgatcgcg cacgagatcc gcaacccgct ggccgcgatc cgctactcgg cgcaactgct
     6361 ggcggaatcg gaagccatcg gcgatgccga ccagcgcatg gtcgagatca tcaacaacca
     6421 ctgcggacgc gtcaacgaga tcatcgagaa catcctgcaa ctctcgcggc gcgagcgctc
     6481 tcgccccgaa tcgctggacc tcaacaactg ggcggcgcgc ttcgtcgatg aatacaagga
     6541 agccaacgag atcggccacg acagcctgcg gccgctgctg tatccgcgcc cggtcgaggc
     6601 gctggccgac acgcagcacc tccagcaggt cgtgtggaac ctggttcaga acgcgctgcg
     6661 ctacgggcgc atgccggacg agcccgcgcg ggtgatggtg gtcgcgcgcc tggccagcga
     6721 caacgggccg ccgctcctgg aagtgatcga ccgcggcccc ggcatcccgc ccaaggtcgc
     6781 cgcgcagatc ttcgagccgt tcttcaccac ccaccagtac ggcaccgggc tcggcctgta
     6841 cctcgccaag cagatgtgcg aggccaacca ggccaacctc gagtacgtgc cggtcgccgg
     6901 cggcggcagt tgcttccgca tcacgctgac cgcgccgctg cgcacgccga tggggagcga
     6961 aacatgatcg ttgacctgcg gcatggcgcg ttcgggcgag ccgcgctacc ctcctgcgcc
     7021 gaggggacgg accaagcggg gaaggcatga ccagacagac ggtactcgtc gtggatgacg
     7081 agcgggacat tcgtgagttg ttgagtatca cgctggggcg catggacctc gacgtcgatg
     7141 ccgcagcgaa tgtcaccgat gcgaagaaga tgctgagcga ggtccgctac gacctctgtt
     7201 tcacggacat gcgcctgccg gacggcaccg ggcaggagct catcgaactg atcgcacgcg
     7261 agcatcccga caccccggtc gcgatgatca ccgcctacgg caacgtcgag gccgccgtcg
     7321 atgcgctgaa ggccggcgcc ttcgacttcg tcagcaagcc ggtggacatc cacatgctca
     7381 ggcgactcgt gcagacggcg ctgcgggtcg gccaggctcc ggaaaccagc gacgaggaag
     7441 cgccggaaac gcggctggtc ggcgaatcgc aggcgctcaa ggaagttcgc tcgcggatcg
     7501 ccaaggtcgc gcgcagccag gcgccgatct acatctcggg cgagtccggg gtcggcaagg
     7561 aactggtcgc ccgctcgatc cacgaactcg gtccgcgctc cgccggtccc ttcgtgccgg
     7621 tcaactgcgg cgcgatcccg tccgagctga tggagagcga attcttcggc caccagaagg
     7681 gctcgttcac cggcgcgcac gccgacaagg aaggcttgtt ccagacggcc aacggcggca
     7741 cgcttttcct cgacgaggtc gcggaactgc cgctgcacat gcaggtgaaa ttgctgcgcg
     7801 tgatccagga aaaggcggtg cgcccgatcg gcggacgcgg cgaaatcccg gtggacgtgc
     7861 gcatcctgtc cgccacccac aaggacctcg cgcgcctcac ccagagcggc cagttccgcc
     7921 aggacctcta ctaccgcatc aacgtgatcg agctgcgggt gccgccgctg cgggagcgcg
     7981 cgggcgacat cccggtcctg gcgacgcaca tcctcaagaa gctcgccgag caggcaggcg
     8041 tcccggtgcc ggccgtgcaa ccggccgcac tcacggcgct ccaggactac gacttccccg
     8101 gcaacgtgcg cgaactcgaa aacgtgctgg agcgcgcgat agcgttgtgc gaaaacggtg
     8161 tcatcgaacc cgccgacctg atgctcgaac ctgccgcgcg tggaacgaac gagggcgacg
     8221 aggacgaaac gctggaggac gacgacgacg cctccggcgc ttcaggcggc gccgtcggac
     8281 tcgacgacta catctccagc atcgaacgcg acgcgatcgt gaaggcgctc aaggaaacgc
     8341 gctacaacaa gaccgccgcg gccaagaacc tcggcatcac gttcagggcg ttgcgctaca
     8401 agctgaagaa gctcggcatc gactgaagcc ctcgcgcggg atcagcctgt cgctgcctcg
     8461 ccacccccag gctgacgctc caaccatgcg atgacggtcg ccaccgcatg gcgcggcgcg
     8521 cgatccgtgc gcacgatcca gtaggcgttt tcgatgcgca gcatgcgctc gccgaacggt
     8581 cgcaccaaca gcccggcggc cacgtcgtcg accgccagcc ggtggcgggc caaggcaacg
     8641 ccttgaccct gcgcggccgc ccgcaacagc aagtccgacg aggcgaagcg cggaccccgc
     8701 tgcaggttca acgaagccgg tccgaaccgc tcgcgccact gctcccagcc tgcatgcggg
     8761 tcgcggtcgt gcaagaggcg caacgaggcc agcgcctgcg ggcgcgaggg gcgtcctgct
     8821 tttttccaga gtcccgggct catcaccggg tacaacgcgt catccatcaa cggttcgcag
     8881 tgcagacccg gccaagggcc ggcgcccatc cgcaccgcaa gatcgatgcc gccggcgcgc
     8941 agatcctcgg tgcgctgatt caccagcacc gacagttcga ttcgcgggtg cgcgcgctcc
     9001 agcgccggga gccgcggcag cagccagcgc gcggcgaacg agggcgcggc ggagatcgtg
     9061 acggcgaacg ccgatcgcac ctcgcggatc gccagcgtcg cagccccgat gtcccgtagc
     9121 ccggccagca ccgccttgcc cagcgcttcg ccctggggcg tcggcgtcca gccgcggcgg
     9181 ccgccgccct tctcccgcgc cagcggcacg cccagccacg cttccagctc ggccagatgc
     9241 cggctcacgg aggaatgcgc gatgcccagt tcgcgcgctg ccgcccgcac gccgccatgc
     9301 gcgcaggcca gcgcgaacac gcgcagcgcg ttcaggggca ggtccttcat ggtctcgata
     9361 ttagaccaaa acggcggcgc acgatccaga cgcggcggcg atcatcgggt tggccgcgcg
     9421 acttgatgga ttcgccgatg acaacgttgt tctcgaccgg tttgttcctg ctgatcgccg
     9481 cgatcacgcc gggccccaac aacctggtgg tgatgcgcgc cgccgcgcgc aacggctgga
     9541 gggccgcctt gcccgcggcg gcgggcgtca tcacgggcgg cctcgtgctg ttggcggtgg
     9601 tgacggcagg tgcggacggc gcatttgccg cgtggccgtg gttgcggggc atgatcgagc
     9661 ttgccggagt gctctacctg gtctggctgg gcacccggat gatcgcggcg gcgggcgaag
     9721 atgccggctc ggcggcactc ccggccgggc tgcccggatt gttcgcgttc cagttcctca
     9781 acccgaaagg ttgggtcatg gtgctcacgg tggtggctgc actgcccggc gccaatggag
     9841 cgcatgcgtt gttgtacctg gcgccgcttt tcgtctgcat tcccgcggca tgcctgctgc
     9901 tgtgggcagg actgggcggc gcgctggcac cgtacctggg ccgccccgtg gtgcgccaat
     9961 ggaccgaccg cgtactcggc gcactactga tcctggccgc gctgctgttg ttcacctgat
    10021 cccgaccgaa ggaggtcttg cacatgagtc tgcaacatcc ttgtagccac cggatacttg
    10081 ccctgcccgg cagcctgcgt cgtggcagtt acaaccgcaa gctgctggag gcttgcggcc
    10141 accgcgcccc cggcaatttg cggataaagc tgttcgacgg cctcgcggac gtgcc
//
LOCUS       DASSNH010000126         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1679832, whole genome shotgun sequence.
ACCESSION   DASSNH010000126 DASSNH010000000
VERSION     DASSNH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679832"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1297)
                     /gene="ribD"
                     /locus_tag="VFK00_04520"
     CDS             complement(20..1297)
                     /gene="ribD"
                     /locus_tag="VFK00_04520"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7592250.1"
                     /translation="MKNDSDPGFSATDHALMARALRLAERGLFTTQPNPRVGCVIARG
                     GEILGEGWHRRAGESHAEVCALHAAGVRARGATAYVTLEPCSHHGRTPPCAEALVHAG
                     VARVVFASGDPNPQVAGAGILRLRQAGVHVDGGLLREHARELNRGFFSRFERARPWVR
                     VKLAMSLDGRTALGSGDSKWITGDPARADVQRWRARSSAILTGAGTARADDPRLTVRL
                     PALEPHSLREWGRGEGSEGSKRHGVAVPPRAPSGHSLPEGEGNSFAPPLRVVLDARLD
                     ALHASAHLLDGSAPTLVLHAPDARVRDDRHGQVELAAVPVDASGRLDLEAVLRLLAAR
                     NVNELQVEAGPALCGALLERGLVDELLLYVAPVLLGDQARPLFALPPLDRMDSRWRLR
                     TIGEHRVGADLRLLLRPDPGGEADAHQPPPAAV"
     gene            complement(1350..1874)
                     /gene="nrdR"
                     /locus_tag="VFK00_04525"
     CDS             complement(1350..1874)
                     /gene="nrdR"
                     /locus_tag="VFK00_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET7592251.1"
                     /translation="MHCPFCQNIDTRVIDSRVSEEGSTIRRRRECPSCGERFSTVETV
                     ELKLPAIIKDDGRREAFDARKLRTGFDRALHKRPVTEDRIEAAVRAVVRQLRMSTERE
                     LPSRRIGELVMTELRKLDHVGYVRYASVYRSFEDVADFREELDRLEHELPASESQLPL
                     IGSSVVPIDKNKKR"
     gene            complement(1983..>2304)
                     /gene="glyA"
                     /locus_tag="VFK00_04530"
     CDS             complement(1983..>2304)
                     /gene="glyA"
                     /locus_tag="VFK00_04530"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260263.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7592252.1"
                     /translation="GGTEDHLFLVDLIGKPITGKDAEAALGKAHITVNKNAVPNDPQK
                     PFVTSGLRIGSPAVTTRGYKETDCIELAGWVADVLDAPKDDKVIENVRALVNAQCARF
                     PVYG"
BASE COUNT          344 a          832 c          762 g          366 t
ORIGIN      
        1 actggccggc gctgcaagtc tacaccgccg cgggcggcgg ctggtgggcg tcggcttcgc
       61 cgcccggatc cgggcgcagg agcagccgca ggtccgcgcc gaccctgtgt tctccgatcg
      121 tccgcaaccg ccagcgcgag tccatcctgt ccagcggtgg cagcgcgaac agcggccgtg
      181 cctggtcgcc gagcagcacc ggcgccacgt acagcagcaa ctcgtcgacc aggccgcgct
      241 ccagcagcgc gccgcacagg gcggggcccg cctccacctg cagttcattg acgttgcggg
      301 cggccaacag gcgcagcacg gcttcgagat ccagcctgcc gcttgcatcg accggcaccg
      361 cggccagttc gacctggcca tggcgatcgt cccggacgcg agcgtccggc gcgtgcaaca
      421 ccagcgtcgg cgcactgcca tcgagcaggt gcgcgctggc gtgcagcgca tcgagtcgtg
      481 cgtccaacac gacgcgcaaa ggtggagcaa agctgttccc ctctccctcc gggagagagt
      541 gtcccgaagg ggcgcgcggg ggtacggcta cgccgtgacg tttcgatcct tccgaaccct
      601 cacccctgcc ccactcccga agggagtggg gttcaagagc cggcagccgc accgtcagtc
      661 gcggatcgtc cgcccgcgcg gtgccggcgc ctgtcaggat cgcagaactg cgcgcccgcc
      721 agcgttgcac gtcagcgcgc gctgggtcgc cggtgatcca tttcgaatcg ccgctgccca
      781 gcgcggtgcg accgtccagg ctcatcgcca gcttcacgcg cacccacggc cgagcgcgct
      841 cgaagcgcga gaagaacccc cggttcagct cgcgcgcgtg ctcgcgcagc aggccgccat
      901 cgacgtggac gccggcttgg cgcaggcgca ggattcccgc cccggccact tgcggattgg
      961 ggtcgccgga cgcgaacacc acgcgcgcaa cgccggcgtg gaccagtgcc tccgcgcacg
     1021 gcggcgtgcg gccatggtgc gaacacggct ccagcgtgac gtacgccgtc gcgccccgcg
     1081 cgcgtacacc ggccgcgtgc agcgcgcata cctccgcatg ggattcgcca gcgcgccggt
     1141 gccagccctc gcccaggatt tccccgccgc gcgcgatcac gcagccgacg cgcgggttgg
     1201 gttgggtcgt gaacaaaccg cgctcggcga ggcgcagggc gcgcgccatc agtgcatggt
     1261 cggtggccga aaaaccgggg tcggagtcat ttttcatgtg gaatgcccct cacccgtcgg
     1321 gtcggctgaa aaatgactct gacccctttt cagcgcttct tgttcttgtc gatgggcacc
     1381 accgagcttc cgatcagcgg caactggctt tcgctggccg gcaactcgtg ctcgagccgg
     1441 tccagttcct cgcggaagtc cgccacgtct tcgaacgagc ggtacaccga cgcataccgc
     1501 acgtacccga catggtcgag cttgcgcagt tccgtcatca ccagctcgcc gatccggcgc
     1561 gacgggagct cgcgctcggt cgacatcctg agctggcgca ccaccgcgcg caccgccgcc
     1621 tcgatgcggt cttcggtcac cggccgcttg tgcaacgcac ggtcgaagcc ggtacgcagt
     1681 ttgcgcgcat cgaaggcttc gcgccgaccg tcgtccttga tgatcgccgg caacttcagt
     1741 tccacggtct ccaccgtgga aaaccgctcg ccgcaggacg gacactcgcg ccgccggcgg
     1801 atggtcgaac cttcctccga aacgcgcgag tcgatcacgc gggtgtcgat gttctggcag
     1861 aaggggcagt gcatcagggc ttggggaccg gggaccgggg accggggacc ggaaatgcca
     1921 tggcgaccgt gcggccccgg cttttttccg gtccccggtc cccggtccct ggtccctgct
     1981 tgtcaaccat aaaccggaaa cctcgcgcac tgcgcattca ccagcgcgcg cacgttctcg
     2041 atcaccttgt cgtccttcgg cgcatccagc acgtcggcga cccagccggc gagttcaatg
     2101 caatcggttt ccttgtagcc gcgggtggtg acggcggggc tgccgatgcg gaggcccgag
     2161 gtgacgaacg gcttctgcgg atcgttcggc accgcgttct tgttgaccgt gatgtgtgcc
     2221 ttgccgagcg cggcctccgc gtccttgccg gtgatcggct tgccgatcag gtcgaccagg
     2281 aacaggtggt cctcggtgcc gccc
//
LOCUS       DASSNH010000127        22090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_390103, whole genome shotgun sequence.
ACCESSION   DASSNH010000127 DASSNH010000000
VERSION     DASSNH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 22090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22090
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390103"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VFK00_04535"
     CDS             <1..1219
                     /locus_tag="VFK00_04535"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET7592253.1"
                     /translation="AAPWTALQQLRQLFDVHRLDSEGLASLQADSVDALVLIQPVAMS
                     QPELQGLDRYVQQGGHLLVFVDPDSEVAAGESSDLPELFGAWGVAFDHARVLLDSSRA
                     LQVQVPGSDARTFDPAVLGLTGDELNRRDPVTAALSFLNVSTTGYFGLVANTATRLDP
                     LIQSTTDAMAVPDARVTGAPDPSALYKDYKPDGKHYAIAVRLEGPLAAAFPGDAAASG
                     QARKPEVILVGDTDLLSERLWAQTSTSQGQTLVSPFANNGDFFVNAVDDLAGPSDLIA
                     IRGRAVAERRFTRVDALRRQADEKFKAKQLELQGELRDTEQRLASLKQAAHGSSQTAA
                     QRAAVDEFLQRKLEIRDELRAVQRSLDADIERLSMLLKFIDILLMPILVALVGLLYGA
                     WRLHRRRVAGEGR"
     gene            1216..1614
                     /locus_tag="VFK00_04540"
     CDS             1216..1614
                     /locus_tag="VFK00_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET7592254.1"
                     /translation="MSAVPPVSRRPPRWPGAAAGIAGASAVVFGALGAHGLQGRLAPA
                     VLDAWEIAVRFQFWHALALVACALLVRRSHAAYLGAVLFVSGIILFCGSLYALALGAP
                     RMIGVFTPLGGLCFIVAWVALGVALFRDRA"
     gene            1826..2512
                     /locus_tag="VFK00_04545"
     CDS             1826..2512
                     /locus_tag="VFK00_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET7592255.1"
                     /translation="MKLAVIARGFDVQAAAAHLGKRDRKLARWMRRIGPIERDFEQRF
                     DPVDALAHSILYQQLSGKAAATIAGRVEALMPRGRRITARGLDGVDDVALRAAGVSRN
                     KIAALRDLAAKSHEGIVPTPARLARMPDQDLIDHLVQVRGIGRWTVEMLLMFRLGRPD
                     VLPIHDLGIRKGAQIVHGFEELPAPLALQEFGELWGPYRTLASFYLWKIVEAANPPKP
                     GAKPTKRSQD"
     gene            complement(2581..5250)
                     /locus_tag="VFK00_04550"
     CDS             complement(2581..5250)
                     /locus_tag="VFK00_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7592256.1"
                     /translation="MTKRMSWLIGALLLAPVAGGALAAPGAQDASVARVTLDNGLRVV
                     VVRDALAPVVTTEMNYLAGSNEAPQGFPGTAHAVEHMMFRGSPGLSRDQLLAIASNMG
                     GEFNAVTHEGATQYYFTTPAQDLDVALRVAATRMRGIDMSPAGWTKERGAIEQEVSRD
                     NSSPIYKFVTQLRAQMFTGTPYAWTGLGTRPSFDKTSAAALKKFHDTWYAPNNAILVV
                     AGDVDPAATVQKIKGLFGDIPRKSLPARPDFDFKPMQAKTVSLPTDLPVGVVALAWRM
                     PGLRDDDYATALVLADAMASKRAALFEMGLTGKALFGQFSGSFMPHAGIAFALAGFPR
                     GGDSATVLKNLQAIMAAAASRGVPPELVAAAKDKAIASLEFQRNSVPGLANAWSEALA
                     FRGGTSPDDIRAAIAAVTPAQVDALARRIFDPAHALTAVLTPESSGKPSTSKGFGGTE
                     TFGGQPSGSVTLPDWAKAAFAKLESPKFATDPTSFTLSNGMRVIVQPESVSKTVEVYG
                     QVETNQDMQAPKGQEGVASVLNDLFNFGTTGMNRLQFQAAVDAISAEENAGSSFSLKV
                     PSANFAKGMQLLAANELDPAMPQQAFTVMQRNQQGAVAGVIQSPGFLHTLHLDQALYP
                     AGDPDLRHATPQTVGALTLQDVRKYYGQAFRPDMTTLVVIGDVTPDQVRKVAESAFGK
                     WKASGPQPATHYPAVPPNKASQFRTPDKSAVQDSVTLAQTIPLTENSPDRYAMNLGNQ
                     ILGGGFDSRLMQDLRVKGGLVYGVSSSVGLQKNRGVFSVDYGSDPDKVAQARAMVIRD
                     IRQMQAAPVSDTELHAAKGKMLRQLALGQSSFGSIAGTLLSLAMQDKPLDSDAIAARK
                     YMALTAKDIQAEFRKDLRPDAFVTAVKGPAAKQ"
     gene            complement(5425..6030)
                     /locus_tag="VFK00_04555"
     CDS             complement(5425..6030)
                     /locus_tag="VFK00_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7592257.1"
                     /translation="MVRESGQAAGSTPPWRCRDAAQHRRLIERAFALGVPRDYGRARH
                     LRLQREPARLVSIGSDIHGREQWLQPAAARALATMRKVASRDGVELQVVSAFRSIEYQ
                     LGILERKLARGLTMDQILRASAAPGYSEHHSGRCVDFTAPGFAPVEEGFEDAAAFAWL
                     QHNAARFAFALSYPRDNRHGIAYEPWHWCWHAKPRHALQAR"
     gene            complement(6038..6532)
                     /locus_tag="VFK00_04560"
     CDS             complement(6038..6532)
                     /locus_tag="VFK00_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HET7592258.1"
                     /translation="MHRKPKAIAMAALLAAPAFAVGQTPPAEASAHPASRAHDNAMAA
                     QADAARIEACSHIARGFLDDLEKDDFIAAVANFNDQMEAAVSADKLGQTWKSLAARFG
                     RLESRGNAQTVMYRDMPVVTTPLHFAKGDLVSQLACDNEGKITGFYVRPLPGAGAAPA
                     SSPG"
     gene            complement(6564..6755)
                     /locus_tag="VFK00_04565"
     CDS             complement(6564..6755)
                     /locus_tag="VFK00_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HET7592259.1"
                     /translation="MTPDTRWTYKVVRFKSSKFFNLHPDPEEVTDLLNQFGRDGWELV
                     HVVEGHGVPSPVLYFKKPT"
     gene            complement(6752..6961)
                     /locus_tag="VFK00_04570"
     CDS             complement(6752..6961)
                     /locus_tag="VFK00_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HET7592260.1"
                     /translation="MGTDKDRKAYPLRISAAVLDAMQRWSNDELRSLNGQIEYVLRDA
                     LRKAGRLKSDATPPHPSSHRNGNRT"
     gene            complement(7037..7903)
                     /locus_tag="VFK00_04575"
     CDS             complement(7037..7903)
                     /locus_tag="VFK00_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7592261.1"
                     /translation="MNETRATSLSGIPFAVFYTIALIFCIAGFVHGALHQALGILIGS
                     VILFVVFAFLCKGFFQVQPNQGMVLLLFGRYVGTARETGLRWTNPFLSKRTVSLRVRN
                     FESSKLKVNDADGNPIEIAAVIVWQVVDTAEAVFQVDDYENYVHIQSESALRQMAQSY
                     PYDSHDNGKPSLRSHGDEITAHLREQIQERLGKAGVQVLEARISHLAYAQEIAQAMLQ
                     RQQAGAIIAARTKIVEGAVTMVEMALAHLNEHNIVQLDEERKAAMVSNLLVVLCGERG
                     TQPVLNTGSLYQ"
     gene            complement(8222..8707)
                     /locus_tag="VFK00_04580"
     CDS             complement(8222..8707)
                     /locus_tag="VFK00_04580"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7592262.1"
                     /translation="MNGRVMRAAMLAAAAVVTVLARAAQPADVAAPMHDAQADKLAVT
                     QLEHRWIADIGSVNRGDLATILADDYRDIDWQGRVRDKDALLASLGKPSGTSQHILAL
                     QVRAWGDTAVATGTNEVHSATKGWTAEVSFTDVFARIDGHWHAVASQETLRKPASGGH
                     G"
     gene            complement(8704..9429)
                     /locus_tag="VFK00_04585"
     CDS             complement(8704..9429)
                     /locus_tag="VFK00_04585"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HET7592263.1"
                     /translation="MTLRNERVRLFQTLPHACGYFRARTAQNLVLDPAAANLPKLYAM
                     SLARGFRRAGGHVYHPHCPRCHACEPCRIDVATFVPDRSQRRCLAANRDVTVVECKPG
                     LTDERQALYARYLHARHPGGGMDEASDEDFSHFLAAPWSPTVFLEFRAKERLVAVAVT
                     DVCETGLSAVYTFFEPSLPKRSLGTYAILRQVDLARTRGLPHLYLGYWIAGHPKMGYK
                     ARFDSLQVLGPDGWTALAGAGRT"
     gene            complement(9429..9824)
                     /locus_tag="VFK00_04590"
     CDS             complement(9429..9824)
                     /locus_tag="VFK00_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592264.1"
                     /translation="MRRIYASPRMENIDRLVAVMAEHGIATKVTNRRAYAGSTYRRFS
                     YARPGHSENWPAVWVKHASDHTRARKLMRDIGIEPVTRYAEELAAYRARKAGGRNDPS
                     HIAARVRTAVLVAVCVAIAIYGAKLISVW"
     gene            complement(9960..10823)
                     /gene="tesB"
                     /locus_tag="VFK00_04595"
     CDS             complement(9960..10823)
                     /gene="tesB"
                     /locus_tag="VFK00_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HET7592265.1"
                     /translation="MNQPHVAELIQLLQLDRLEDNLFRGQSRDIGTRYVFGGQVLGQA
                     LSAAQQTVDPTRAAHSLHGYFLRAGDVDKPIVYSVERARDGHSFSVRRVEAIQNGQPI
                     LNCAVSFQVEEAGFEHQTGMPEVPGPDDLAPPTPLPAAELAKLPPKLRRWLSTDGPFE
                     YRFVWPRDELHPAKRPPWQHIWFRLAERIDAPPALHRALLAYASDYHLIGTALLPHGV
                     SYLTPNLQMASLDHALWFHRPFRADEWLLYSCESPTAQGGRGLVRGQMFDVSGKLVAS
                     TAQEGLLRLRS"
     gene            complement(10926..11495)
                     /locus_tag="VFK00_04600"
     CDS             complement(10926..11495)
                     /locus_tag="VFK00_04600"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7592266.1"
                     /translation="MHAPLRIRDDLLPYVERLQRRERASIDQVVIHCTELPDLATARE
                     YGERIVHPSGTGNSGHYYIDRDGSVFRYVPDDRVAHHTRGYNERAIGIELVNVGRWPD
                     WFDSHRQVMSEPYPDAQIDALTALLAELAVKLPALREIAGHEDLDTDMVPASDDADRM
                     VRRKLDPGILFPWGKVLARCPLRRHASAP"
     gene            11576..12112
                     /locus_tag="VFK00_04605"
     CDS             11576..12112
                     /locus_tag="VFK00_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7592267.1"
                     /translation="MPGHVILSHGSDSGPGATKVSALARVAESLGWSTLRPDFRDVDA
                     RGYAGSVGPRIERLVAAMRDAPRPLVLVGSSMGAFVSGLASLEAPCEGLFLIALPMDI
                     PQCPRRFAAADGVRGMLVHGWRDELCAPDAALAFARARSMPALMLDGNHRLADHVAYI
                     ERQFRWFLESLASERRAD"
     gene            12239..14404
                     /gene="rlmKL"
                     /locus_tag="VFK00_04610"
     CDS             12239..14404
                     /gene="rlmKL"
                     /locus_tag="VFK00_04610"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HET7592268.1"
                     /translation="MTEFFATCPKGLEYLLRDELTALGASAQEALAGVRFGGDIECAY
                     RACLESRLASRILLPVTEFETNDADTLYAGAHAVDWAQHFAPDATLAIDATGTSGSIV
                     HSGFAAQRVKDAIADQCRERFDARPSIQPERPDVRLNLRLHKGRATLSVDMSGEPLHR
                     RGWRRGQGEAPLKENLACAMLLRARWPEIHAGGGALLDPMCGSGTLLVEAAWMAAGVA
                     PALRRDYFGFLGWRGHDAALWQKLHEAAEARALEGLRNLRPCFFGSDSDPQMIATSTH
                     NLQNAGVAGFAHLDKRPVEHLEPPPGFATGLVITNPPYGARMGDPAALPSLYEALGAR
                     LREGFVDWHAAVLTSEPELVHALGLRPAKRYVLFNGAIECRLALFDIHAPALAAAPAP
                     LSPGAEMVANRIRKNLRNMRKELSRDGVTCFRAYDADLPEYAAAIDVYRGWPEDAPSI
                     GEPADTQTWLHVQEYAPPASIDTEAALKRWRELQRAACDALDVPRSRLVLKTRARGRG
                     GSRYGKLAARDEYLVVEEGGLHFLVNLRDRLDTGLFLDHRRVRARLRELARGRDMLNL
                     FAYTGTASVYAAAGGARSTTSVDLSPVYLDWASRNLVLNGQTGAAHQLVQADAMAFLR
                     GGRTRYGLVYVDPPTFSNSARAEDFDVQRDHVELLEACGKRLRDDGVIVFSNNYRRFK
                     LDADALSRTFAIEDIGRTSIPPDFARRPRIHGCWLLRKR"
     gene            14786..15181
                     /locus_tag="VFK00_04615"
     CDS             14786..15181
                     /locus_tag="VFK00_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592269.1"
                     /translation="MNRARKVSAGIRRRGRRVVVGAALLVVAVCAAPAALAHSHVGIS
                     IGVGNCWGCGYRTPPPVYYAPAYPPPVYYPPTVYYASPVYYGYTYGYAPYYPYPRQGR
                     GHYYPRHGGHHGQGGWNHGGHGHYGRGHH"
     gene            15231..15920
                     /locus_tag="VFK00_04620"
     CDS             15231..15920
                     /locus_tag="VFK00_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7592270.1"
                     /translation="MNRFTEPSVDEQRAGEVALDLHSMRTFLRQWLRDPVKMASVTPS
                     GRQLARLMVAQLPEGSPRVVEIGAGTGVFTRALLDYGIEPSRLLVVEINPHLADFLRA
                     RFPGVAVVCADARHLDRLAADHGLLAEGEPDAVVSGLGLLSMSGELRSEILRAAFAVL
                     ADEGRFIQFTYGPRSPVRTHERDALGLEVRRAGSVLRNLPPASVYVYRRRAAQASRAA
                     ASPTQDSSDNA"
     gene            complement(15896..16381)
                     /locus_tag="VFK00_04625"
     CDS             complement(15896..16381)
                     /locus_tag="VFK00_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592271.1"
                     /translation="MATTEAGRPSFVTVVRSTPADLDAASHLFDAYRVFYKQPSDPQG
                     AHDFLRARTERGQSEIFLARDAATGDLLGFTQLYPSFSSVSMRAVWVLNDLFVEPGAR
                     RRGVARALMEAAREFAHATGALRLVLETAEDNVQAQALYESLGYRREAGTRHYALSLE
                     S"
     gene            complement(16466..16996)
                     /locus_tag="VFK00_04630"
     CDS             complement(16466..16996)
                     /locus_tag="VFK00_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592272.1"
                     /translation="MSMPRSIRTSLAAAVAFGLSGVTSLAMAQAMPQQPTMQQVPPPP
                     PPPPPQAPPPPPPPPSVTPPQPAPMTTMPAQPQPAGQDQQQGSSVEFYQGTQSSANYP
                     IQNGTLTVNAGMPANVRDYGPPPPFKTLDTNSDGRISQAEAQAYPPLDSDFLFASRQA
                     QTISQAQYQRWVQGQH"
     gene            complement(17145..20714)
                     /gene="mfd"
                     /locus_tag="VFK00_04635"
     CDS             complement(17145..20714)
                     /gene="mfd"
                     /locus_tag="VFK00_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7592273.1"
                     /translation="MPSSNIPVPPIPRAAGQRRDWREPAGSSLALLLCEAARGRDGVV
                     VAVTADSRGARLLEDELQFFAGDLQVLHFPDWETLPYDLFAPHPQLVSQRIDTLYRLP
                     ATRSGVLVVPASTLMQRLAPRSHIAGAGLAVARGERLDLADEQRRLEASGYRHVPQVG
                     EHGEFAVRGALLDVFPMGADEPYRIELFDDAIESIRAFDPETQRSLHQVEAVKLLPAR
                     EFPLTDEAMRAFRDRLRERFPIDVRRCPLYQDMKQGVTPGGIEYYLPLFFEATETLFD
                     YLGEEALLVVERGALDAAEHFWQQTADRYDQRAHDIERPILPPMELYLPPQSLRERLN
                     RTLRVDIVAGDDARAAESDTQPAPELSGAGVAAGGFPPRMRAWLDANPGRRVLIAADS
                     AGRREALIDQLATAELRPEVVESWRAFAAPPPHRGDEESTRTASSTPPLRGPVGTQRL
                     QREGGLSGGGQLSITCAPLDRGFSLVEPALTILTERELAGERARTERQRRRTAERDPE
                     AILRDLTELEIGAPIVHVDHGVGRYLGLVALDVGGMPGEFLAIEYARGDKLYVPVAQL
                     ALVSRYSGADDEHAPLHSLGGESWERAKRKAAEKVRDVAAELLAIHAERAARQGNAIE
                     YDRAMVARFAEGFPFEETPDQEAAIEAVLADLAAPQPMDRVVCGDVGFGKTEVALRAA
                     VAVASAGKQVAVLVPTTLLAQQHFDSFRDRLADFPVRVELLSRFRAKKETGEALQRLA
                     DGKLDIVVGTHKLLQADVKFKDLGLVIVDEEHRFGVRHKEQLKKLRAEVDLLTLTATP
                     IPRTLNMAMSGMRDLSIIATPPAARMAVKTLVAQYDPALVREAFQRELARGGQVYFLH
                     NSIDSIQRIARELAALVPDARIRVAHGQMSAHELEQAMLDFHRQRFNVLVCTTIIESG
                     IDVPTANTIVIDRADRFGLAQLHQLRGRVGRSHHRAYAYLIVPDKKAMTADAGKRLAA
                     LESLEELGAGFTLATHDMEIRGAGELLGEAQSGQIEAIGFALYNELLERAVRALRSGK
                     VPDFDLGGDGGSEIELHLPSLIPDDYLPDVPARLTLYKRIASATDEDALRDLQVEMID
                     RFGLLPQPAKNLFAIAELKLRAAALGIRKLDLGAAGGRITFRPRPEVDPATIIRLIQR
                     QPRVYKLDGQDKLRVAMELPEAADRLRAAGELLEALAVRSKAA"
     gene            complement(20808..21311)
                     /locus_tag="VFK00_04640"
     CDS             complement(20808..21311)
                     /locus_tag="VFK00_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592274.1"
                     /translation="MQSTETIQLGDGRCFVLRPLRPGDIEALRRAFLRLTTDEVEYRF
                     FHRPSELPASVMAQVRDLDLALDAAFVVDDAGEIRAVADLHVADSGAREAEFGLIVGK
                     AISGHGIGRRLMLRLLEEARRRGLASLVGNVRADNARMIELCRDLGATITRMPEEPNT
                     LCVKFAP"
     gene            complement(21600..22088)
                     /locus_tag="VFK00_04645"
     CDS             complement(21600..22088)
                     /locus_tag="VFK00_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET7592275.1"
                     /translation="MLLSLALPAAIAACAPAPIYKTGGPSSVMATPQQVATSPANFQN
                     LEVVWGGEVIGVHNLADHSEVEILAYPLDSSQRPRLKEPPTGRFLAIVPGFVEPMNFP
                     PGTLVTLRGLLQGAREGEVGRAPYTFALVHSEGMHRWTPEEMRQGHPNISIGVGIGGW
                     IH"
BASE COUNT         3391 a         7268 c         7663 g         3768 t
ORIGIN      
        1 ggcggcgccg tggacggcgc tgcaacaact ccggcaattg ttcgacgtgc accgcctgga
       61 ctccgaggga ctggcctcgt tgcaggccga cagcgtggac gcgctggtgc tgatccagcc
      121 ggtggcgatg tcccagccgg aattgcaggg gctggaccgc tacgtgcagc agggcgggca
      181 cctgctggtg ttcgtggatc ccgattcgga agtggcggcc ggcgagtcat ccgatttgcc
      241 cgaattgttc ggggcatggg gtgtggcatt cgatcacgcg cgggtactgc tcgacagttc
      301 gcgtgcgctg caggtgcagg tgccgggcag cgacgcgcgt acgttcgatc cggcggtgct
      361 gggcctgacc ggcgacgaac tcaaccgccg cgacccggtc actgcggcgc tcagtttcct
      421 caatgtttcc accaccgggt atttcggcct cgtggcaaac acggcgacac ggctcgatcc
      481 gctgatccag agcacgaccg acgcgatggc cgtgccggat gcgcgcgtga ccggtgcgcc
      541 cgatccttcg gccctttaca aggattacaa acccgacggg aagcactacg ccattgccgt
      601 gcggctcgaa ggaccgttgg cggcggcctt cccgggcgat gcggcggcgt cggggcaggc
      661 gcgcaaaccg gaggtgatcc tggtgggcga caccgacctg ttgtcggagc ggctgtgggc
      721 gcaaacgagt acgtcgcaag gccagacgct ggtgagcccg ttcgccaaca atggcgactt
      781 cttcgtcaac gcggtggatg acctcgccgg gccgtccgac ctgatcgcga tccgcgggcg
      841 cgcggtggcg gagcggcgct tcacccgcgt ggacgccctg cgccggcaag ccgatgaaaa
      901 attcaaggcc aagcagctcg agctgcaggg ggaactgcgg gacaccgaac agcgcctcgc
      961 gtcgctcaag caagccgcgc acggcagcag ccagacggct gcgcagaggg ccgcggtcga
     1021 cgagttcctg caacgcaagc tggagatccg cgacgaactg cgtgccgtgc agcgaagtct
     1081 cgacgccgac atcgaacgtt tgtccatgct cctgaaattc atcgacatcc tgctgatgcc
     1141 gatcctggtg gcgctggtgg gactgctgta tggcgcatgg cgcctgcaca ggcgccgcgt
     1201 ggccggggaa gggcgatgag cgccgtgccg ccggtaagcc ggcgaccgcc ccgctggccc
     1261 ggcgcggcgg ccggaatcgc cggcgcaagc gccgtggtgt tcggcgcgct gggcgcgcac
     1321 gggcttcagg gcaggctggc cccggcggtg ctggacgcgt gggagatcgc ggtgcgcttc
     1381 cagttctggc atgcgctggc gctggtggcc tgcgctttgc tggtgcggcg ctcgcacgcc
     1441 gcatatctcg gcgcggtgct gttcgtttcc gggatcatcc tgttctgtgg cagcctctac
     1501 gcgcttgcgc tcggcgcgcc gcgcatgatc ggggtgttca cgccgcttgg cggattgtgt
     1561 ttcatcgtgg cgtgggtcgc gctcggcgtg gcattgttcc gcgatcgtgc ctgatcgcgg
     1621 caccgtcgaa tgacacgatc gccgggatcg tccgcgggtg ccgaaacacc agaacggcca
     1681 tgccaggcct gaccttccag gacggcggct acgccggtga agagtcatgg cggtctgggt
     1741 gccccctgag tgtacactcc gcccgccgcg ccgccgaacg aggcgttgcc ggcatgatgt
     1801 tgaacagata cttcaggaga actcgatgaa attggcggtg atcgcacgcg gattcgacgt
     1861 gcaggcggct gccgcgcatc tcggcaagcg cgaccgcaag ctggccaggt ggatgcgccg
     1921 gatcggtccg atcgagcgcg acttcgagca gcgtttcgac ccggtcgacg cgctcgcgca
     1981 ttcgatcctg taccagcagc tgtccggcaa ggccgcggcg acgatcgccg gccgcgtcga
     2041 ggcgctgatg ccgcgtggcc gccggatcac ggcgcgcggc ctggacggcg tggacgacgt
     2101 cgcgctgcgc gcggcgggcg tgtcgcgcaa caagatcgcg gcgttgcgcg acctcgccgc
     2161 gaaatcgcac gaagggatcg tgccgacacc cgcgcggctg gcgcggatgc cggaccagga
     2221 cctgatcgat catctggtcc aggtgcgcgg catcgggcgc tggaccgtcg agatgctgtt
     2281 gatgttccgg ctgggccggc cggacgtgct gcccatccac gacctcggca tccgcaaggg
     2341 cgcgcagatc gtgcacgggt tcgaagaact tccggcgccg ctcgcgctgc aggaattcgg
     2401 cgagctgtgg gggccgtacc gcacgctggc aagtttttat ctctggaaga tcgtcgaagc
     2461 cgccaacccg ccgaagcccg gcgccaagcc caccaagcgt tcgcaggatt gagccgcgcc
     2521 ggccggcgcg gcccatggcc cgtttgccgg tgtcgtcgct cggtacggca acaacgggcg
     2581 ctattgcttg gccgcaggtc ccttcaccgc cgtgacgaag gcgtccggcc gcaggtcctt
     2641 ccgaaactcc gcctggatgt ctttcgccgt cagcgccatg tatttccggg cggcgatcgc
     2701 gtccgagtcg agcggcttgt cctgcatcgc caggctgagc aacgttcctg caatggaacc
     2761 gaagctcgac tggcccaagg caagctgccg cagcatcttg cccttcgccg cgtggagttc
     2821 ggtgtctgac acgggcgcgg cctgcatctg cctgatgtcg cggatcacca tggcacgcgc
     2881 ctgcgcgacc ttgtcgggat cgctgccgta gtcgacgctg aagacgccgc ggttcttttg
     2941 caggccaacg ctgctggaaa cgccgtagac caggcctccc ttgacgcgca ggtcctgcat
     3001 cagccgcgaa tcgaagccgc cgccgaggat ctggttgccg agattcatcg catagcgatc
     3061 cggactgttc tcggtgagcg ggatcgtttg cgccagcgtc accgaatcct gtaccgccga
     3121 cttgtcgggc gtgcggaact ggctcgcctt gttcggagga accgccggat agtgcgtggc
     3181 gggttgcggg ccgctggcct tccatttgcc gaacgccgac tccgccacct tgcgcacctg
     3241 gtcgggcgtc acgtcgccga tgacgacgag cgtggtcatg tccggccgga acgcctgccc
     3301 gtagtacttc cggacatcct ggagcgtcag ggcccccacc gtctgcggcg tcgcgtggcg
     3361 gaggtccgga tcgcctgcgg gatagagcgc ctggtccagg tggagggtgt gcaggaagcc
     3421 cggcgactgg atcacccccg cgaccgcacc ttgctggttg cgctgcatca ccgtgaacgc
     3481 ctgttgcggc atcgccggat cgagttcgtt ggcggcgagg agttgcatgc ccttggcgaa
     3541 gttcgccgat ggcaccttca gcgagaagct gctcccggcg ttttcctcgg cgctgatcgc
     3601 atccaccgcg gcctggaact gcaggcgatt catgccggtg gtgccgaagt tgaacaggtc
     3661 attcaacacg gatgcgacgc cctcctggcc cttcggcgcc tgcatgtcct ggttggtctc
     3721 gacctgcccg tacacctcca cggtcttgct gaccgattcg ggctggacaa tcacgcgcat
     3781 gccgttcgac agggtgaacg aagtcgggtc ggtcgcgaac ttcggcgact ccagcttcgc
     3841 gaacgcggcc ttcgcccaat ccggcagcgt caccgagccg ctcggctggc cgccgaacgt
     3901 ttcggtgcca ccgaatccct tgctcgtgga aggcttgccg gatgattccg gtgtgagcac
     3961 cgccgtgagc gcgtgggcgg ggtcgaagat gcgcctggcc agcgcgtcga cctgcgcggg
     4021 cgtgaccgcg gcaatcgcgg cacggatgtc gtcgggcgag gtaccgccgc ggaacgccag
     4081 tgcctcggac caggcattcg ccaggcccgg caccgagttg cgctggaact ccaggcttgc
     4141 gatggccttg tccttcgcgg ccgccacgag ctccggcgga acacccctgg atgccgccgc
     4201 ggccatgatg gcctggaggt tcttcagtac cgttgcggaa tccccgccgc gcggaaagcc
     4261 cgccagcgcg aacgcgatac ccgcgtgcgg catgaaactg ccgctgaact ggccgaacag
     4321 ggccttgccc gtcaggccca tttcgaacag cgcggcgcgt ttggatgcca tcgcgtcggc
     4381 gagtaccagc gccgtcgcgt aatcatcgtc gcgcagtccc ggcatccgcc acgcaagcgc
     4441 caccacgccg accggcaggt ccgtgggcaa gctgacggtc ttcgcctgca tcggcttgaa
     4501 gtcgaagtcc gggcgagcgg gcaggctctt gcgggggatg tcgccgaaca agcccttgat
     4561 cttttgcacc gtcgccgccg gatcgacgtc acccgcgaca accaggatcg cattgttcgg
     4621 tgcgtaccac gtgtcgtgga acttcttcag cgccgcggcc gaggtcttgt cgaacgacgg
     4681 ccgcgtgccg aggccggtcc aggcatacgg cgtgccggtg aacatctgcg cgcgcaactg
     4741 ggtcacgaac ttgtagatcg gacttgagtt gtcgcgcgag acctcctgct cgatcgcgcc
     4801 gcgctccttg gtccagcccg ccgggctcat gtcgatgccg cgcatccggg tcgccgcgac
     4861 gcgcagggcg acgtcgagat cctgcgccgg cgtcgtgaag tagtactggg tggcgccctc
     4921 gtgggtgacg gcgttgaact cgccgcccat gttcgatgcg atggccagca actggtcccg
     4981 ggacaggccc gggctgccgc ggaacatcat gtgctcgacc gcgtgcgcgg tgccggggaa
     5041 cccttgcggc gcctcgttcg agcccgccag gtaattcatc tcggtcgtca cgacaggcgc
     5101 cagcgcatcc cgcaccacta ccacgcgcag gccgttgtcc agcgtcacgc gtgcgacgct
     5161 tgcgtcctgc gcaccaggcg cagccaacgc gccgcccgcc accggcgcca gcagcagcgc
     5221 cccgatcagc caactcattc gtttcgtcat ggtcccctgt ccattgcaat cacgtgctca
     5281 atgaatagaa ccatacgcga cggcgggtca agtcttgatc tccaaaatgc cgagcggcac
     5341 gtgcgtgcgc gtaacagatg aatctttcat cgacctttca gaagatgaac cagggtaacg
     5401 agcgctgggc cgcgaccgga atcgctaccg cgcctgcaaa gcatggcgcg gcttggcgtg
     5461 ccagcaccag tgccagggct cgtacgcaat cccgtggcgg ttgtctcgcg gataggagag
     5521 tgcgaacgcg aagcgcgcgg cgttgtgctg cagccaggcg aatgctgccg cgtcttcgaa
     5581 tccttcttcc accggcgcaa agccgggtgc cgtgaaatcg acgcagcggc cactgtggtg
     5641 ctcgctgtag ccgggcgccg cgctggcgcg gaggatttga tccatcgtca gaccgcgtgc
     5701 gagtttgcgc tcgaggatgc cgagctggta ttcgatcgaa cggaaggcgg agaccacctg
     5761 cagttcgacg ccatcgcgtg acgcgacctt gcgcatcgtc gccagcgcgc gcgccgcggc
     5821 cggctgcagc cattgttcgc ggccgtggat gtcgctgccg atcgacacca gccgtgccgg
     5881 ctcgcgttgc aggcgcaaat gccgcgcgcg gccatagtcg cgcggtacgc cgagtgcgaa
     5941 ggcgcgctcg atcagtcgcc ggtgttgggc ggcatcgcgg caacgccacg gtggcgtgga
     6001 accggcggcc tggccggact cccggaccat gccgccatca ccctggcgac gatgcgggtg
     6061 ccgcgcccgc acccggcagc ggccggacgt agaacccggt gatcttgcct tcgttgtcgc
     6121 acgcaagctg cgacacgaga tcgccctttg cgaaatgcag cggcgtggtg acgaccggca
     6181 tgtcccgata catcacggtc tgcgcgttgc cgcgcgactc gagtcggccg aatcgggcgg
     6241 ccagcgactt ccacgtttga ccgagcttgt ccgcgctgac ggcggcttcc atctggtcgt
     6301 tgaagttcgc gaccgcggca atgaagtcgt ccttttcgag atcgtccagg aatccacgcg
     6361 cgatgtggct gcaggcttcg atacgggcgg catcggcctg cgccgccatg gcgttgtcgt
     6421 gcgcgcgcga ggcggggtgc gcgctggcct ccgcgggtgg cgtctgcccg acggcgaaag
     6481 ccggcgcggc gagcaacgcg gccatcgcga tggctttcgg tttgcggtgc atggtggttc
     6541 cttttttgcg gtggagggcg gcttcaggtc ggctttttga agtagagcac gggcgagggt
     6601 acgccatggc cctcgaccac gtggaccagt tcccagccgt cacgtccgaa ctggttcagc
     6661 aaatcggtga cctcctcggg atccggatgc aggttgaaga acttcgagga cttgaagcgg
     6721 acgaccttgt aggtccagcg cgtgtcgggt gtcatgttcg gttcccgttt cgatgtgacg
     6781 atgggtgggg cggcgtggca tcggacttga gtcgcccggc cttgcgcaac gcgtcgcgga
     6841 gcacgtattc gatctgcccg ttcagcgagc gcagctcgtc gttcgaccag cgttgcatcg
     6901 cgtcgagcac cgccgcgctg atgcgcagcg gataggcctt cctgtcctta tcggtgccca
     6961 tgcggcatca ggacgcgcgc tgttgtggaa agtcggccat ggatcgccgt cttttgatct
     7021 ccgtgatcat ggatgatcac tgatacaaac tccccgtatt cagcaccggc tgcgtgccgc
     7081 gctcgccgca cagcaccacc agcaggttgg acaccatcgc ggccttgcgt tcctcgtcga
     7141 gttgcacgat gttgtgctcg ttgaggtgcg cgagcgccat ttcgaccatg gtgaccgcgc
     7201 cttccacgat cttggtgcgc gcggcgatga tcgcgccggc ctgctggcgt tgcagcatgg
     7261 cctgcgcgat ctcctgggcg taggcgaggt ggctgatgcg cgcttccagc acctggacgc
     7321 cggccttccc caaccgctcc tggatctgct cgcgcaggtg cgcggtgatc tcgtcgccgt
     7381 ggctgcgcaa gctgggcttg ccgttgtcgt gcgaatcgta ggggtagctt tgcgccatct
     7441 ggcgcaacgc cgactcgctc tggatgtgca cgtagttttc gtaatcgtcc acctggaaca
     7501 ccgcttcggc ggtatcgacc acctgccaca cgatcacggc ggcgatctcg atcgggttgc
     7561 cgtcggcgtc attgaccttg agcttggacg actcgaagtt gcgcacccgc agggacaccg
     7621 tgcgcttgct gaggaaggga ttggtccagc gcaagccggt ttcccgcgcc gtaccgacat
     7681 atcggccgaa cagcagcaac accatgccct ggttgggctg gacctggaag aagcccttgc
     7741 aaaggaacgc gaagaccacg aacaggatca ccgagccgat caggatgccg agcgcctggt
     7801 gcagggcgcc atggacgaaa ccggcgatgc agaagatcag ggcaatggtg tagaaaaccg
     7861 caaagggaat gccggacagg gaagtagctc gggtctcgtt cattgggttc tccgcttggg
     7921 tgacaaaacg atatcacaat gatatctatt cgccaagcgg gttgctcggc cacgaacgtg
     7981 ggtcgcggtc gggccgccac tccaaacagc aggcctgcgg gagggtgcca tgcgcgccat
     8041 gggggctccc gtgggggcgc aagcaagaaa tgtcacgcgc atttggtggt tgctgaatcc
     8101 ctgtcgagtc aatgtgttgc cgaacgtgtg ttcggcgcag tcgagctcgc gtgccgggta
     8161 cacgcgtcga cgggcaacgt gggaccggtc agcgagtacc cgccccgaca gcggcgaggg
     8221 ctcagccgtg cccgccggaa gctggcttgc gcagcgtttc ctgcgaggcg acggcgtgcc
     8281 agtgtccgtc gatgcgtgcg aacacgtcgg tgaaggaaac ttcggccgtc caaccctttg
     8341 tggcggagtg gacctcgttg gtgccggtcg cgacggcggt gtcgccccat gcgcgcacct
     8401 gcagggcgag gatgtgctgg gaggttcccg atggcttgcc gagggaggcc agcagcgcgt
     8461 ccttgtcccg gacacggcct tgccagtcga tgtcgcggta gtcgtccgcg aggatcgttg
     8521 cgagatcccc gcggttgacg ctgccgatgt cggcgatcca gcggtgctcc aattgcgtta
     8581 ccgcgagctt gtcggcctgc gcgtcgtgca tcggggccgc aacgtcggcc ggttgagcgg
     8641 cgcgcgccag gaccgtcaca acggcggccg cggcgagcat ggccgcgcgc atgacacgcc
     8701 cgttcacgtc cgacctgcgc ccgcgagggc cgtccagccg tccggcccga gcacctgcag
     8761 gctgtcgaag cgcgccttgt aacccatctt gggatggccc gcgatccagt atccgaggta
     8821 caggtgcggc aaaccgcgcg tgcgcgccag atcgacctgg cgaaggatcg cgtaggtgcc
     8881 gaggctgcgc tttggcaggc tgggttcgaa gaacgtgtac acggccgaca ggccggtttc
     8941 gcacacgtcg gtgacggcga ccgcaaccag ccgctccttc gcgcggaact ccaggaatac
     9001 cgtggggctc cacggcgcgg ccaggaaatg ggaaaagtct tcatcgctgg cttcgtccat
     9061 cccgccgccg gggtggcggg cgtgcaggta gcgcgcgtac aacgcctgtc gctcgtcggt
     9121 caggccgggc ttgcactcga ccaccgtgac gtcgcggttc gcggcgaggc agcggcgctg
     9181 gctgcgatcg gggacgaacg tggcgacatc gatccggcac ggttcgcagg catggcagcg
     9241 cgggcagtgc gggtggtaga cgtgcccgcc ggcgcggcgg aaaccgcgcg ccagcgacat
     9301 cgcatagagc ttcggcaggt tcgccgcggc cggatctagc accaggttct gcgccgtgcg
     9361 cgcacggaag tagccgcagg catgcggcag ggtctggaac agccggacgc gttcgttgcg
     9421 aagtgtcatt accagaccga gatcagcttc gctccgtaga tggcgatggc gacgcacacc
     9481 gccacgagta ccgcggtgcg tacccgcgcg gcgatatgcg aggggtcgtt ccggccgccg
     9541 gccttgcggg cgcgataggc ggcgagttct tcggcgtagc gggtcaccgg ctcgatcccg
     9601 atgtcgcgca tcagcttgcg cgcgcgcgta tggtcgctgg cgtgcttcac ccacaccgcc
     9661 ggccagttct cgctgtggcc ggggcgtgcg tacgagaaac gccggtaggt ggagcccgcg
     9721 taggcgcggc gattggtgac cttggtcgcg atgccgtgtt cggccatcac ggcgaccagc
     9781 cggtcgatgt tttccatccg tggcgaggca tagatcctgc gcatgcggac agtttacgac
     9841 agcccccggg ggttgcaatc gggtggcgtc gcggcgttgt ggaacgcgtc gaaaatgcag
     9901 ggaggacacc gtccgccacg accgtgacgg gcaatgcccg ccctgccgtt atccggaggt
     9961 caactgcgca gccgaagcaa tccttcctgc gcggtcgacg ccaccagctt gccggaaaca
    10021 tcgaacatct gtccgcgcac cagcccgcgc ccgccttgcg cggtcggact ttcgcaggaa
    10081 tacagcagcc attcgtccgc gcggaacggg cggtggaacc acaacgcgtg gtcgaggctc
    10141 gccatctgca ggttgggggt gaggtaggac acgccgtgcg gcaacagcgc ggtgccgatc
    10201 aggtggtaat cggacgcgta cgccagcagt gcgcggtgca gggcgggcgg cgcgtcgatt
    10261 cgctcggcca gccggaacca gatgtgctgc cagggcggcc gtttggccgg atggagctcg
    10321 tcgcgcggcc acacgaagcg atattcgaat ggtccatcgg tgctcaacca ccgccgcagc
    10381 ttgggcggca gcttggcgag ttcggcggcg ggaaggggag tgggcggcgc aaggtcatcc
    10441 ggacccggca cttccggcat gcccgtctgg tgctcgaatc cggcttcctc gacctggaac
    10501 gacaccgcgc aattcaggat cggttgcccg ttctggatcg cctcgacgcg ccgcacggaa
    10561 aagctgtggc cgtctcgtgc gcgctccacc gaatacacga tgggcttgtc gacatcaccg
    10621 gcgcgcagga aatagccgtg cagcgaatgt gccgcgcgcg tcggatcgac ggtctgctgc
    10681 gccgcggaca gtgcctgccc cagcacctgg ccaccgaaca cgtagcgcgt gccgatgtcg
    10741 cggctctggc cacggaagag gttgtcctcc aggcgatcca gttgcagcaa ctggatcaat
    10801 tcggcgacgt ggggctggtt cattgcggca agccgggcaa aaagtccgtg gattatagat
    10861 ggcgacatgc gagcggcctg ctcgtcgcgt gcgccgtgcg gcggacgacg caacaccgct
    10921 cggcatcatg gcgcggaagc gtgccttcgc aacgggcagc gagccagcac ctttccccag
    10981 gggaacagga ttccggggtc gagcttgcgc cgcaccatgc gatcggcgtc gtcgctggcc
    11041 ggcaccatgt cggtgtcgag gtcctcgtgg cccgcgattt cgcgaagcgc gggcagtttc
    11101 accgcgagtt ccgccagcaa cgccgtgagc gcgtcgatct gcgcatcggg atacggctcc
    11161 gacatcacct gccggtggga gtcgaaccag tcgggccagc gaccgacatt gaccagctcg
    11221 atgccgatcg cgcgttcgtt gtagccgcgg gtgtggtgtg ccacgcgatc gtccggcaca
    11281 tagcggaaca cgctgccgtc gcggtctatg tagtagtgcc cgctgttgcc ggtgccgctc
    11341 gggtgcacga tccgctcgcc gtattcgcgc gccgtggcga ggtcgggcaa ctccgtgcaa
    11401 tgaatcacca cctggtcgat gcttgcgcgc tcgcgccgct gcagccgttc cacgtacggc
    11461 agcaggtcgt cacggatccg tagcggcgca tgcatcgcga aagtgtcgca cggattggtg
    11521 gcggccgcgg atcgcctgct acgctttgcg tctcgctttc cccgcctccc cgccaatgcc
    11581 cggccacgtc atcctctccc acggctccga cagcggaccc ggcgccacca aggtcagcgc
    11641 gctcgcgcgg gttgcggaat cgctcggctg gagcacgctg cgccccgact tccgcgacgt
    11701 ggatgcccgc ggctacgcag ggtcggtcgg accgcgcatc gaacggcttg tggccgcgat
    11761 gcgcgatgcg ccgcgaccgc tggtgctggt cggctcgagc atgggcgcat tcgtgtcggg
    11821 cctggcgtcg ctcgaggcac cgtgcgaggg tttgttcttg atcgcgctgc ccatggatat
    11881 cccgcaatgc ccgcggcgtt tcgcggcggc ggacggcgtg cgcggcatgc tcgtgcacgg
    11941 ctggcgcgac gagttgtgcg cgccggatgc agcgctggca ttcgcccgcg cgcgctcgat
    12001 gcctgcgctg atgctcgatg ggaaccatcg gctggcggac cacgtggcgt acatcgagcg
    12061 ccaattccgc tggttcctcg aatcgctggc atcggagcga cgcgccgatt gacgcgcatg
    12121 ggagaggcga caggcttttg acgcggatcc gcgcggatca atgcgataaa tgcttctgat
    12181 ccgggtctat ctgatttgat ccgcgtccat ttcgttttgc attcttgctc gaacaccaat
    12241 gactgaattc ttcgccacct gtcccaaggg cctcgagtac ctgttgcgcg acgaactgac
    12301 cgcgttgggg gcgtcggcgc aggaagcctt ggcgggcgtg cgtttcgggg gcgacattga
    12361 gtgcgcctat cgtgcgtgcc tggaatcgcg tctcgcgagc cggatcctgt tgccggtcac
    12421 ggaattcgaa accaacgatg ccgatacctt gtacgccggc gcgcacgcgg tcgactgggc
    12481 gcagcacttc gcgcccgatg ccacgctcgc gatcgatgcg accggcacca gcggctcgat
    12541 cgtgcattcg gggttcgccg cgcagcgggt gaaggatgcc attgccgacc agtgccgcga
    12601 acgtttcgac gcacgcccga gcatccagcc cgaacggccg gacgtgcgcc tgaacctgcg
    12661 cctgcacaag gggcgcgcca cgctctccgt ggacatgtcg ggcgaaccgt tgcaccggcg
    12721 cggctggcgg cgcggacagg gcgaggcgcc gctcaaggag aacctcgcct gcgcgatgtt
    12781 gttgcgcgca cgctggccgg aaatccatgc cggcggcggt gcgttgctcg atccgatgtg
    12841 cggctccggc accttgctgg tcgaggcagc ctggatggct gccggtgtcg cgccggcgct
    12901 gcggcgcgat tatttcgggt tcctcgggtg gcgcggccac gatgctgccc tgtggcagaa
    12961 gctgcacgaa gcggccgagg cgcgcgcgct ggagggactt cgcaatctgc gcccgtgctt
    13021 cttcggcagt gacagcgacc cccagatgat cgcgacctcg acgcacaacc tgcagaacgc
    13081 gggggtcgcc ggtttcgccc atctcgacaa acgcccggtc gaacacctcg agccaccgcc
    13141 gggcttcgcg accgggctgg tgatcaccaa tccgccctac ggagccagga tgggcgatcc
    13201 cgcggccttg ccgtcgctct acgaggcgct gggtgcgcga ctgcgcgagg ggttcgttga
    13261 ctggcacgca gcggtgctga cctcggagcc tgaactcgtg cacgcgctag gcctgcgccc
    13321 cgcgaaacgc tacgtgctgt tcaacggcgc gatcgaatgc cggcttgcgc tgttcgacat
    13381 tcacgcgccc gcacttgccg cagcgcccgc gccgctttcg cccggcgcgg agatggtcgc
    13441 caaccgcatc cgcaagaacc tgcgcaacat gcgcaaggag ctctcgcgcg acggcgtcac
    13501 gtgctttcgc gcctacgacg cggacctgcc ggagtacgcg gcggcgatcg acgtgtaccg
    13561 cggctggccg gaagatgcgc cctccatcgg cgagccggcg gacacgcaaa cctggctgca
    13621 cgtgcaggag tacgcgccgc ccgcgagcat cgataccgaa gccgcgctga aacgctggcg
    13681 cgagctgcaa cgggccgcgt gcgatgcgct cgacgtgccg cgcagccggc tggtcctgaa
    13741 aacgcgcgcc cgcggccgcg gcggcagccg ctacggaaag cttgccgcga gggatgaata
    13801 cctggtcgtg gaggagggtg gcctgcattt cctggtgaac ctgcgcgatc gcctcgacac
    13861 cggtttgttc ctcgaccatc gccgggtgcg cgcgcggctg cgcgaactcg cgcgtggccg
    13921 cgacatgctc aacctcttcg cctacaccgg caccgcgagc gtgtacgccg ctgcgggcgg
    13981 ggcgcgcagc accaccagcg tggacctttc gccggtatac ctcgactggg cgtcgaggaa
    14041 cctggtcctc aacgggcaaa ccggcgcggc gcaccagctc gtgcaggccg acgcgatggc
    14101 gttcctgcgc ggaggccgca cgcgttacgg tttggtctac gtcgatccac cgaccttttc
    14161 gaactccgcc cgcgccgagg atttcgacgt gcagcgcgac cacgtcgagc tgctcgaagc
    14221 ctgcggaaag cgcctgcgcg acgacggcgt catcgtgttt tccaacaact accgccgctt
    14281 caagctggac gcggacgcgt tgtcgcgcac gttcgcgatc gaggacatcg gacgcacgag
    14341 cattccgccg gatttcgcgc gccgtccgcg catccacggc tgctggctgc tgcgcaaacg
    14401 ctgatcctgg gtgccgggca tcgtacgctc agcgccaacc tgcaagagca tggcggcgcc
    14461 gcctccttgg tggtttcgta ggttgggttg atcccggaac tcgttccggg agaaggccaa
    14521 cagggcgtgg tcaaccgatt gctcgaatcg gtcgacgatc gttgggcttc gcttcgctca
    14581 gcaccaacct acaaaagctt tccgacaccg cccgctacat agctttcgta ggttgggttg
    14641 atcccggaac tttgttccgg gagaagccca acagggtcgc ctcgcgcgtt gggcttcact
    14701 gcgctcagcg ccaacctgca agagtacgct ttgtctgtgg gctggtttca gtcgtgattc
    14761 agtgcccggc cgcaagcatg cccgcatgaa ccgagcacga aaagtttccg ccggcattcg
    14821 tcggcgcggc cgtcgcgtgg ttgtcggggc agcgttgctg gtcgttgcgg tgtgcgccgc
    14881 gccggctgcc ctggcgcata gccatgtcgg catttcgatc ggcgtcggca attgctgggg
    14941 ctgcggctat cggacgcccc cgccggtgta ctacgccccc gcgtatccgc cgcccgtcta
    15001 ttacccgccc accgtgtatt acgcatcgcc cgtgtattac ggatacacct acggctacgc
    15061 gccgtactac ccgtatcctc gccagggccg aggccactac tatccgcgcc atggcgggca
    15121 ccacggacag ggggggtgga accacggcgg tcacggccac tacggccgcg gccatcattg
    15181 atgcgccggt cgcggtctga cccaatgcgg tgtgataaaa ggtcgcaccc atgaaccggt
    15241 tcactgaacc ttccgtggat gaacaacgcg ccggtgaggt cgccctcgac ctgcactcga
    15301 tgcgcacgtt cctgcgccag tggctgcgcg atccggtgaa gatggcatcg gtcacgccat
    15361 cgggccgtca gttggcgcgc ctgatggtgg cgcaattgcc ggaaggcagc ccgcgcgtcg
    15421 tggaaatcgg cgcgggtacc ggagtgttca cccgtgcgct gctcgattac ggaatcgaac
    15481 cgtccaggtt gctggtggtc gaaatcaatc cacacctggc cgatttcctg cgcgcgcgct
    15541 ttccgggcgt ggcggtggtg tgcgccgacg cgcgccacct cgatcgcctc gcagccgacc
    15601 atggcctgct cgcggagggc gaaccggacg cggtggtcag cgggctcggg ctgctctcga
    15661 tgagcggcga gcttcgcagc gagatcctgc gcgcggcctt tgcggtgctc gccgacgagg
    15721 gcaggttcat ccagttcacc tacgggccac gcagtccggt ccggacgcac gagcgcgacg
    15781 cgttgggact cgaggttcgg cgcgcgggtt cggtgctgcg caaccttccg cccgcgtcgg
    15841 tgtatgtcta ccggcgccgg gctgcgcaag cgtcgcgagc ggcggcatcg ccgactcagg
    15901 attcgagcga taacgcgtag tgccgcgttc cggcttcgcg ccggtatccc agcgactcgt
    15961 acagcgcctg cgcctggaca ttgtcttccg cggtttcgag caccagccgc agcgcaccag
    16021 tcgcgtgggc gaattcgcgc gcggcctcca tcagggcgcg tgccacgccg cggcgtcttg
    16081 cccccggctc gacgaacaag tcgttaagca cccacaccgc ccgcatcgat accgatgaga
    16141 acgaaggata gagctgggtg aatcccagga ggtcgccggt cgcggcatca cgcgcgagga
    16201 agatctccga ttggccgcgc tcggtacgcg ctcgcaggaa gtcatgcgcc ccttgcgggt
    16261 cggagggctg cttgtagaac acccggtagg catcgaacaa atgcgacgcc gcatcgagat
    16321 cggcgggcgt cgagcgcacg acggtgacga aggatggacg cccggcttca gtggttgcca
    16381 tgcatggtcg cgtcggccgt gggaccgcga tcatgcccgc gcgccgcgcc cgtacgcaag
    16441 cgcggcgcgc gcttctagcg acggctcaat gctggccctg cacccaccgc tggtactggg
    16501 cctggctgat cgtctgggcc tggcggctcg cgaacaggaa gtcgctgtcg agcggcggat
    16561 acgcctgcgc ttcggcctgg ctgatccggc cgtcggaatt ggtatccagg gtcttgaacg
    16621 gcggcggcgg accatagtcg cggacattcg cgggcatgcc ggcgttcacg gtcagcgtgc
    16681 cgttctggat cgggtagttc gcgctcgatt gggtgccttg gtagaactcg acggacgagc
    16741 cctgctgctg atcctgcccg gccggttgcg gttgcgccgg catggtggtc atgggcgctg
    16801 gctgtggcgg agtcactgac ggcgggggag gcggtggcgg cggtgcttgc ggcggcggcg
    16861 gcggaggcgg cggtggaacc tgctgcatcg tcggctgctg cggcatggcc tgcgccatcg
    16921 cgagcgacgt gacaccgctc aggccgaacg cgaccgcggc tgcgagtgac gtgcgaatcg
    16981 aacgtggcat gctcatggga actcctttgg gaatgcggcg caaggcgtgt ggcctgcgcc
    17041 accgttggac cgcggcggga gcggcgggtt tcgctccggg ccggcaacga tcagcttgca
    17101 ttcaagcgcg tgacggcggc gtcacagggc gccgccgggc gcgttcaagc ggctttgctg
    17161 cgcaccgcga gcgcttccag cagttcgccg gccgcgcgca ggcggtcggc ggcctccggc
    17221 aattccatcg cgacacgcag cttgtcctgg ccgtcaagct tgtagacgcg cggctgccgc
    17281 tggatcaacc ggatgatcgt ggcgggatcg acctccgggc gcggccggaa cgtgatgcga
    17341 ccgccggccg cgcccaggtc gagcttgcgg atcccgagcg ccgcggcccg gagcttgagc
    17401 tccgcgatcg cgaacaggtt cttcgcgggc tgtggcagca ggccgaagcg gtcgatcatc
    17461 tccacctgca ggtcgcgcag cgcatcctcg tcggtggcgc tcgcgatgcg cttgtacaac
    17521 gtcaggcggg cgggcacgtc gggcaggtag tcgtccggga tcaacgaggg caggtggagt
    17581 tcgatctcgc tgccgccgtc gccgcccagg tcgaagtccg gcaccttgcc ggaacgcagg
    17641 gcgcgcaccg cacgctccag gagctcgttg tagagggcga agccgatcgc ctcgatctgg
    17701 ccggactgtg cctcgcccag caactcgccc gcgccgcgga tttccatgtc gtgggtggcc
    17761 agggtgaaac cggcgccgag ttcctccagc gactccaggg ccgcgaggcg tttcccggcg
    17821 tccgcggtca tcgccttctt gtcgggcacg atcaggtacg cgtaggcgcg gtggtgcgaa
    17881 cggccgaccc gcccccgcaa ctggtgcaac tgggcgaggc cgaaacggtc ggcgcggtcg
    17941 atcacgatgg tgttcgcggt gggtacgtcg atgcccgact cgatgatcgt ggtgcacacc
    18001 agcacgttga agcgctggcg gtggaaatcc agcatcgcct gctcgagttc gtgcgccgac
    18061 atctggccgt gggcgacgcg gatccgcgcg tcgggaacca gtgcggccag ttcgcgcgcg
    18121 atgcgctgga tgctgtcgat gctgttgtgc aggaaataca cctgcccgcc gcgcgcgagt
    18181 tcgcgctgga aggcttcgcg gaccagcgcc ggatcgtatt gcgccaccag cgtcttgacc
    18241 gccatccgtg cggccggcgg cgtggcgatg attgagaggt cgcgcatgcc gctcatcgcc
    18301 atgttgaggg tgcgcgggat cggcgtggcc gtcagcgtga gcaggtccac ttcggcgcgc
    18361 agcttcttga gctgttcctt gtggcggacg ccgaagcggt gttcctcgtc cacgatcacg
    18421 agcccgaggt ccttgaactt cacgtccgcc tgcagcagct tgtgcgtgcc cacgacgatg
    18481 tcgagcttgc catcggccag ccgctgcagc gcttcgccgg tttccttttt cgcgcggaac
    18541 cgcgacagca gttccacccg caccggaaag tccgcgagcc ggtcgcggaa gctgtcgaag
    18601 tgctgctggg cgagcagggt ggtcggtacc agcaccgcga cctgcttgcc cgcgctcgcc
    18661 accgcgaccg cggcgcgcaa cgcgacctcg gtcttgccga agccgacgtc gccgcacacc
    18721 acgcgatcca tcggttgtgg ggccgcgagg tccgcgagca ccgcctcgat cgcggcttcc
    18781 tggtcgggcg tttcctcgaa cggaaaacct tcggcgaaac gcgccaccat cgcgcggtcg
    18841 tattcgatcg cgttgccctg gcgggcggcg cgctcggcgt ggatcgccag gagttcggcc
    18901 gcgacatcgc gcaccttctc ggcggccttg cgcttggccc gttcccagga ctcgccgccc
    18961 agcgaatgca gcggcgcgtg ctcgtcgtcg gcacccgaat agcggctgac cagggcgagt
    19021 tgcgccaccg gcacgtacag cttgtcgccg cgtgcgtatt cgatcgcgag gaattcgccg
    19081 ggcattccgc cgacatccag cgccacgagc ccgaggtagc ggcccacgcc gtggtcgacg
    19141 tgcacgatcg gtgcgcctat ttcgagctcc gtcaggtcgc gcaggatcgc ctcgggatcc
    19201 cgttccgcgg tgcggcggcg ctgccgctcg gtgcgggcgc gctcgccggc cagttcgcgc
    19261 tcggtgagga tggtcagcgc cggctcgacc agcgaaaagc cccggtcgag cggcgcgcag
    19321 gtgatggaca gttgtccgcc gccggaaagt cccccctcgc gctgcaggcg ttgcgtgccg
    19381 accggtcccc gcaggggagg ggtactggaa gcggtgcggg tactttcttc gtctccccgg
    19441 tggggcgggg gagcggcgaa ggctcgccag gattcgacga cctccgggcg cagttccgcc
    19501 gtggccagtt gatcgatcag ggcctcgcgg cgcccggccg aatcggcggc gatcagcacg
    19561 cggcgcccgg gattggcgtc cagccacgcg cgcatgcgcg gggggaatcc gccggcggcg
    19621 acgccggcgc cggagagttc gggcgccggc tgtgtatcgc tctcagcggc gcgcgcgtcg
    19681 tcgccggcca cgatgtccac gcgcaacgtc cggttcaggc gctcgcgcag cgactgcggc
    19741 ggcaggtaca gctccatcgg cggcaggatc gggcgctcga tgtcgtgcgc gcgctggtcg
    19801 tatcggtcgg cggtctgctg ccagaagtgt tcggcggcat ccagcgcccc gcgttcgacc
    19861 acgagcaggg cttcctcgcc caggtaatcg aacagtgtct cggtcgcctc gaagaacagc
    19921 ggcaggtagt actcgatgcc gcccggcgtc acgccctgct tcatgtcctg gtagagcgga
    19981 cagcggcgca cgtcgatcgg aaaccgttcg cgcaggcggt cgcggaacgc gcgcatcgcc
    20041 tcgtcggtga gcgggaattc gcgcgcgggc aacagcttca ccgcctcgac ctggtgcagc
    20101 gagcgctggg tttcgggatc gaaggcgcgg atggactcga tcgcgtcgtc gaacagctcg
    20161 atgcggtagg gctcgtccgc gcccatcgga aacacgtcga gcagcgcgcc gcgcacggcg
    20221 aattcgccgt gctcgcccac ctgcggaaca tggcggtagc cgctggcctc caggcgccgt
    20281 tgctcgtcgg cgaggtcgag acgctcgccg cgggccacgg cgagccccgc gccggcaatg
    20341 tggctgcgcg gcgccagccg ttgcatcagg gtcgatgcgg gcaccaccag cacgccgctg
    20401 cgcgtcgccg gcaggcggta caaggtatcg atccgctgcg acaccagttg cggatgcggc
    20461 gcgaacaggt cgtagggcag ggtttcccag tccgggaagt gcagcacctg caggtcgccc
    20521 gcgaagaact ggagctcgtc ctcgagcagg cgcgcgccgc ggctgtcggc ggtcaccgcg
    20581 acaaccacgc cgtcgcgccc gcgcgccgct tcgcacagca gcaacgcgag cgaagaaccg
    20641 gccggttcgc gccaatcgcg gcgctggccg gcggcacgag gaatgggcgg tacggggatg
    20701 ttcgatgaag gcacgtgggc aatccgggca aaacaccacg ctgccggagc gcatggcgcg
    20761 ccagcagagc atggacgaca cgccatttta ccagtggtcg cccgggttca tggggcgaat
    20821 ttcacgcaca acgtgttggg ctcctccggc atccgcgtga tcgttgcccc gaggtcgcgg
    20881 caaagctcga tcatgcgggc gttgtcggcg cggacgttgc cgaccagcga agcgagtcca
    20941 cgtcgcctgg cttcctcgag caggcgcagc atcaggcgac ggccgatgcc atggcccgaa
    21001 atcgccttgc cgacgatcag cccgaattcg gcttcccgcg cgccgctgtc agcgacatgg
    21061 aggtccgcca cggcacggat ctcgcccgcg tcatccacga cgaacgccgc gtcgagcgca
    21121 agatcgagat cccgcacctg cgccatcacc gacgcgggca attcgcttgg gcggtggaag
    21181 aaccggtatt cgacttcatc ggtcgtcagt cgcaggaagg cgcggcgcaa cgcctcgata
    21241 tcgcccggcc gcagcgggcg cagcacgaag catcgcccgt cgccaagctg gatcgtttcc
    21301 gtggattgca aacgttcctc cgccattacg aatcatgggg cgtgcatcat gtaccgacgg
    21361 aacgttcgtc ggagcggccc atggccgcga ccggggtttg tgtagtaccg atcgggtgca
    21421 tggcgcgctc ctgcaacgaa gatgatgttg tacgaccgcc gcgcggagca gcgatcatgg
    21481 acgatcgcac gctgccgcga aaagccaggg catggatgcc cgttctggtc ttcatggggc
    21541 gggaacggtc aagttcaacg accgcttcgc ggagcagcga tcatggacga tcgcaaaaat
    21601 cagtgtatcc agccgccgat cccgacgccg atactgatgt tcggatggcc ttgccgcatt
    21661 tcctcgggcg tccagcggtg catgccttcg ctgtgcacga gtgcgaacgt gtacggcgcg
    21721 cgcccgactt ccccctcgcg ggcgccctgg agcaggccgc gcagtgtcac cagcgtgccc
    21781 ggcgggaaat tcatgggctc gacgaagccg ggcacgatcg cgaggaagcg tccggtcggg
    21841 ggttccttca ggcgcggtcg ctgcgaggaa tccagcgggt aggccaggat ctcgacctcg
    21901 ctgtggtcgg ccaaattgtg gaccccgatg acctcgccac cccagacgac ttccaggttc
    21961 tggaaattgg cgggcgacgt ggcgacttgc tgcggcgtgg ccatcacgct ggacgggccg
    22021 cccgtcttgt agatcggcgc cggtgcgcag gccgcgatcg ccgcgggtaa tgcgagcgac
    22081 agcagcagcc
//
LOCUS       DASSNH010000128        11495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1272032, whole genome shotgun sequence.
ACCESSION   DASSNH010000128 DASSNH010000000
VERSION     DASSNH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272032"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFK00_04650"
     CDS             complement(<1..1085)
                     /locus_tag="VFK00_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET7592276.1"
                     /translation="MNSQAAQADETRINQARAHAAQHRIAEAEAVYRDILADAPDTPE
                     ALNFVAMCALSRGEFAIAQRDLEQAARVNPTEPEIWKNLGIVHLAQHAFGDALDAFDR
                     ALGIEPVHFAARLHRGAALEQLGRVDDATAAYFGALVAAQTHGKWRNDLTTPPGLRPA
                     VKHAIAFVEAHRRRIFMDLLQPLRERYGASSLARVEQGLAIYLGDRPANYPDPRQFCK
                     FFYVPGLRATPYYERELFPWHAELEKHTDTIREELRGVLDQPIGIEPFLGTNDNTLLK
                     EQNLLAGTDGDAQWNSFFFHRHGEVFEQNARRCPRTTEILSTLPLVHIRGHAPEVLFS
                     ILTPGSHILKHHGVTNSRLVTHLPLIIP"
     gene            complement(1346..2440)
                     /locus_tag="VFK00_04655"
     CDS             complement(1346..2440)
                     /locus_tag="VFK00_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7592277.1"
                     /translation="MQTTQRTRRPTPFDRRRVRRLRSVRLPLRILVLLAIATVLVLAQ
                     SLLIPLVLAAFIALGLNPIVAGLNRLYVPRPLGALLVMVLLAGILAGTAVGVSAPAAK
                     WIREAPHIVQDASYKLRRMTRPIAKVSKAASESLAGVNGQPAVATATEPLGGLSFHNI
                     AKAAPGIVVAVLTVALLVFFFLSYGRDVAAHVVTAMPGFGYRRVALRLIRGIQAEFSR
                     YLLTVTIINCCLGLLTAAIMWALDVPSPLLWGGLATLLNFMPYVGAVTNTLLLVVVGL
                     LNFPTPLQALAPAACFAVLAALEGNVITPMIMGTRMRLSPLAILVWLLIWAWMWGIPG
                     ALLAVPMLTCLKLITEWLPGWQWFAKMVER"
     gene            complement(2754..3131)
                     /locus_tag="VFK00_04660"
     CDS             complement(2754..3131)
                     /locus_tag="VFK00_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592278.1"
                     /translation="MDNEISNEAGRTTAAHERIERTAARVKEGTSSVVGGAREKFDSA
                     AERVESGLHRATDATARGAERATEKAAELRERSAELAADARQRTDAALGSVREFVREK
                     PVQSVAIALAAGWLLGRLLSPRR"
     gene            complement(3292..3924)
                     /locus_tag="VFK00_04665"
     CDS             complement(3292..3924)
                     /locus_tag="VFK00_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7592279.1"
                     /translation="MQIHSNSFQNGATIPAEFAFGKPGTGGEKIALAGNRNPHLAWSG
                     APAAAKSFALACIDGDAPSRGDDVNKEGRTVPADLPRVDFAHWLMVDIPSTCATIAAG
                     ACSDGVTPHGKRQPEGPAGSRQGRNDYTGWFASDPDMQGEYFGYDGPCPPWNDALPHR
                     YRFRVYALDVPRLDLPDVFGWPELQEALRGHVLGEAEWLGVYSINPEAGR"
     gene            complement(4022..4147)
                     /locus_tag="VFK00_04670"
     CDS             complement(4022..4147)
                     /locus_tag="VFK00_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592280.1"
                     /translation="MSGSSCRAIVLAAVLQPLRDGSLLVSDDGNGVIYRITYEPK"
     gene            complement(4361..6451)
                     /locus_tag="VFK00_04675"
     CDS             complement(4361..6451)
                     /locus_tag="VFK00_04675"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592281.1"
                     /translation="MLEELLQLHRNGRLDEAEDGYRRLLADNPDNPEVLHLLGILRGQ
                     RGDLPEAKKLVQRAGELDPHNAASRHTLGEMYLAEGRLEEACAAYDQARQLNPNLSAA
                     HAGLGQVALQRGELDSAESHFKVALRADENDAQALTGLGDIAQKRGDPQRALQLLTQA
                     AGLAPADPVIQTSYARAMFDQGMIDFAAKAVDNALTVKPDYPLARTLRAELHVRRREF
                     APALAIYESLLARGEQVGPARTGIGDIARAQGRLDEAIAQYDDALRVQPGLHPAAIRR
                     ADALARRGRLPQAIDDLRWHVASYPDSPRAHAQLAHLLTRAGRYDEALSVWGQAEARW
                     PDDIELKAQHALTLDRAGRSDEALAMADAAASSNRPALVMLRARAALAAGDPAAAVQR
                     LQRIDDASLANKPPQLARRRQRLLGLACDALEQWPDAAQAFLRAQRLGRGDLPALPLL
                     DDALREDLQRLAGEAELADARGEAPVFLCGLPGSGVGQVAALLADQPGWFVRRERFET
                     APDFVNAPFEPSLLQPLDQPELAMLARRYRRPLQRAGVEDSRRVVDWIPVLDARVVPA
                     IRRALPGARLVIVQREPHDMFIDWLGFGWSEGFAMPEPVAAARWLRLGMSHLQFAASV
                     LPSFRVDPDQLVAASSDTRLRLGVFLHRDDLEPGPLARGARRGRGGLPVSFPVGHSAH
                     YREVFSEAFAVLDG"
     gene            complement(6753..7745)
                     /locus_tag="VFK00_04680"
     CDS             complement(6753..7745)
                     /locus_tag="VFK00_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7592282.1"
                     /translation="MRDVVAADFRPPWWLKSPHVQSILASSALRRFVRRRHSRPLERD
                     AEWTTLDCGDGVRLTGCHTAQRKLPRPRGLAVLFHGWEGSTRSTYLLQTGARLLEEGW
                     DVFRLNFRDHGGSHSLNRGIFHSCRIDEVVGALRALQSLYPVRPMTLTGFSLGGNFAL
                     RVALRAPGAGVDLAHVVAVCPVINPHSGLFQLEQAPRMYHDYFMWKWRRSLQAKQRAF
                     SETELFTRHDLRGSLRTLTEALVLRHTDFGSLDNYLDGYSVAGDRLAGLTVPTSILAS
                     RDDPVCPASDLEHMELPASVTLTVTERGGHCGFIRDRHLDSFAEDFIASRLALA"
     gene            complement(7742..8479)
                     /locus_tag="VFK00_04685"
     CDS             complement(7742..8479)
                     /locus_tag="VFK00_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7592283.1"
                     /translation="MWRCTWRLTLLLLVVIVGCLAAIPLLPRGPKPGTAGASLLTNWS
                     RIFLRIAGVRVQRHGEPLHDPVMFVANHGSWMDITVLHAVRPADFVAKAEIGNWPLVG
                     WMAKRGGTIFHRRGNTGSLTAVMAVMSERLRDGRSIAAFPEGGTAPAGTIKVFHARIL
                     QAALDAAAPVQPVALRYLRDGRPSPEVLFTPGESFLHNVFRVLAVPSLTAEVHFLKPV
                     PFDAEGGRRRMAETARAEIAQVLGLST"
     gene            8617..9504
                     /gene="phhA"
                     /locus_tag="VFK00_04690"
     CDS             8617..9504
                     /gene="phhA"
                     /locus_tag="VFK00_04690"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HET7592284.1"
                     /translation="MNTPHRVEHQHTDRGYIPVYATGIVEQPWNDYTPTDHEVWATLF
                     RRQREILKDRACREFLENQERFGMSPDAIPKFTDLNAVLKAGTGWEIVGVEGLLPDEV
                     FFDHLANRRFPVSWWIRKPEQLDYLSEPDLFHDLFGHVPMLLNPVFADYVQAYGRGGV
                     KAFKLGPDALMNLTRLYWYTVEFGLIDSPDGLRIYGSGIVSSKGESVYCLESESPNRI
                     GFDLERIMLTRYKIDTYQQTYFVIDSFEQLMDATRPDFTPIYARLKDQPAFPAGDVQP
                     GDRVIHRGTGEGWQKTDDV"
     gene            complement(9627..10319)
                     /locus_tag="VFK00_04695"
     CDS             complement(9627..10319)
                     /locus_tag="VFK00_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HET7592285.1"
                     /translation="MPSRDPPLHRLRWQRAYRIVPSRFPPVGVFDAIADGADIEAIYR
                     IEALTNPRLREEWGELSNVPKEHRVSGPGTTPLMAAFTHVNPEGTRFSDGSYGVLYLA
                     HEFDTAVEETVYHRERFLAATHEPPIDVTMRTYVTGVHGMLHDIRGGWKAEHDPTSYV
                     ASRELGAELRKEGSNGIVYDSVRCKGGECAALFYPDVARPCVQGKHLMYRWDGEKIAQ
                     VLEVSAVKRPHP"
     gene            complement(10541..10957)
                     /locus_tag="VFK00_04700"
     CDS             complement(10541..10957)
                     /locus_tag="VFK00_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HET7592286.1"
                     /translation="MKQQAERIRDADGIYRTEGEADLAGPALRAFFRLAERWKLRVAD
                     QRKLLGDPPESTFYKWKRERAGTLSRDTLERISYVLGIFKALAILFPQPDRADAWLRS
                     PNDAPTFGGRTALDRMLSGNVADLYVVRQYLDAQRG"
     gene            complement(11028..>11495)
                     /locus_tag="VFK00_04705"
     CDS             complement(11028..>11495)
                     /locus_tag="VFK00_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET7592287.1"
                     /translation="ELEALVVGTRFVRAFAGNRLAGGAQSALLKIDAVLPPELRGRAE
                     RSRVFVPQRWYEAKSGIVDALHAAIAARQVLRVEYQDADGATSQREIEPLCLACWGPV
                     WTLGAWCRLRGDFRNFRPDRMRFTATGEVFPDVPERGLDAYLAQAGVPHRQIE"
BASE COUNT         1821 a         4008 c         3898 g         1768 t
ORIGIN      
        1 gggatgatca gcggcaggtg cgtgaccagc cgcgaattgg tgacgccgtg gtgcttcagg
       61 atatgcgagc cgggcgtcag gatcgagaac agcacttccg gcgcgtggcc gcggatgtgc
      121 accagcggca gcgtcgacag gatttcggta gtgcgtgggc agcgccgcgc gttctgctcg
      181 aacacctcgc cgtggcggtg gaagaagaac gagttccact gcgcgtcgcc gtcggtgccg
      241 gccagcaggt tctgttcctt caggagcgtg ttgtcgttgg tgccgaggaa cggctcgatg
      301 ccgatcggct ggtccagcac gccccgcagt tcttcgcgaa tcgtgtcggt gtgtttttcg
      361 agttcggcat gccagggaaa cagctcgcgc tcgtaatagg gcgtcgcgcg cagcccggga
      421 acgtagaaga acttgcagaa ctggcgcggg tccggatagt tggcgggacg atcgccgagg
      481 tagatcgcga ggccctgctc gacccgcgcc agcgaggatg cgccgtatcg ctcgcgcagc
      541 ggctgcagca ggtccatgaa gatccggcgg cggtgcgctt ccacgaacgc gatcgcgtgc
      601 ttgacggccg ggcgcagacc gggcggcgtg gtcaggtcgt tgcgccactt gccatgggtc
      661 tgtgccgcga ccagcgcacc gaaatacgcg gcggtggcgt cgtcgacgcg gccgagttgt
      721 tccagcgccg caccgcgatg gagccgcgcg gcgaagtgca ccggctcgat acccagcgcg
      781 cggtcgaagg catccagcgc gtcgccgaag gcgtgctgag ccaggtgcac gatgcccagg
      841 tttttccaga tctcgggctc tgtcgggttg acgcgcgcgg cctgctccag gtcgcgctgc
      901 gcgatcgcga attcgccccg cgacaacgcg cacatcgcga cgaaattcaa cgcttcgggc
      961 gtatccggcg cgtccgccag gatgtcgcgg tacaccgcct cggcctccgc gatgcggtgc
     1021 tgcgcggcat gagcgcgcgc ctggttgatg cgggtttcgt cggcctgcgc cgcctgtgaa
     1081 ttcatgtccc cattttgccg cgtcccgggc ggcggcgctt gattcggcgc aagcgagcgt
     1141 cacccccttt ccgcaggaag ggggtcgccg cgaagcggcg ggggatgcac cggcaaacga
     1201 ggcgtggcca cggttcgtgg cgcgaatgga gcgcggctgc atccgggcta cctgccaacg
     1261 caagaccgcc ggtcgcgcga tggcgcgctc ctgcaaaagg cccgtccgtc tctccgtctc
     1321 tacgtcgctc cgcttcccac gctcttcacc gctccaccat cttcgcgaac cactgccagc
     1381 cgggcagcca ttcggtgatg agcttgaggc aggtgagcat cggtaccgcc aggagcgcgc
     1441 cggggatgcc ccacatccac gcccagatca gcagccacac gaggatcgcg agcggcgaaa
     1501 ggcgcatccg ggtgcccatg atcatcggcg tgatcacgtt gccttcgagc gcggctagca
     1561 ccgcgaagca cgcggcgggc gcgagggcct gcagcggcgt cgggaagttc agcagtccca
     1621 ccaccaccag cagcaaggta ttggtgacgg cgccgacgta gggcatgaag ttcagcagcg
     1681 tcgccaggcc gccccacagc agcgggctcg gtacatcgag cgcccacatg atcgcggcgg
     1741 tcagcaagcc gaggcagcag ttgatgatcg tgaccgtgag caggtagcgc gaaaactcgg
     1801 cctggatgcc gcggattagc cggagcgcga cccgccggta cccgaacccg ggcatcgccg
     1861 tcaccacgtg cgccgcgacg tcgcgcccgt agctcaaaaa gaagaacacc agcagcgcca
     1921 ccgtcagcac cgcgaccacg atgcccggcg cggccttggc gatgttgtgg aacgaaagtc
     1981 cgcccagggg ctccgtggcg gtcgcgaccg cgggctgccc gttgacgccc gccagcgatt
     2041 cggacgccgc cttgctgacc ttcgcgatcg ggcgcgtcat ccgccgcaat ttgtagctgg
     2101 catcctggac gatgtgcggc gcctcgcgga tccacttcgc ggcgggtgca ctcaccccca
     2161 ccgcggtgcc ggcaaggatc ccggccagca ggaccatcac cagcagggcg ccaagcgggc
     2221 gcggaacgta caggcggttc agcccagcga cgatcggatt gagccccagg gcaatgaacg
     2281 cagcgaggac cagcgggatc agcagcgact gcgcgagtac cagcacggtc gcgatcgcca
     2341 gcagcacgag gatgcgcaac ggcaggcgca ccgagcgcag ccggcgtacc cgccggcgat
     2401 cgaagggtgt cggccggcgg gtccgttgcg ttgtctgcaa tggccgtggc tggatcggag
     2461 ggcgaacgcc cgcgccctgg tatctacccg acgatcttga cggcggcatt gcggctcccc
     2521 tccgctgcgg gtgctggcca atggctcggg gtcattgttg cacccgtcgc catgagagcc
     2581 aggtgaaggc gcaacgcggc ggcgcatgcc gtcgacacga gtggtcacca gcgccgtgca
     2641 accgggcggc aagccctgcc cgccattgcg cgtcgccgga ttcccgtcgg cccgctgtcc
     2701 accccgggcc gccttccagc ccccgcaccc ccttccggtt tacgacgcgt acgtcagcgg
     2761 cgtgggctca gcagccgtcc cagcaaccat cccgcggcca acgcgatcgc gaccgactgc
     2821 accggcttct cgcgcacgaa ttcgcgcacg ctgccgagcg ccgcgtcggt ccgctggcgc
     2881 gcatcggctg cgagttccgc gctgcgttcg cgcaattcgg cggccttctc ggtcgcacgc
     2941 tcggccccgc gggcggtggc gtcggtcgcg cggtgcaagc cggattcgac gcgttcggcc
     3001 gcgctgtcga atttctccct ggcgccgccg acgacgctgc tggtgccttc cttgacccgc
     3061 gccgccgtac gctcgatgcg ttcgtgcgca gcggtggtgc gtcctgcttc gttgctgatt
     3121 tcgttgtcca tggtccttct cctgattgtc acgcccgtcc ggcgcgatga agcctttcgc
     3181 caagccccct cttcggggcc acggaatcct tccgcaatac gatcctgcgg tagccgggct
     3241 gaaccggaaa acacccgccg gaacgggtcc cttccggagc gccacgcggg ttcatcgccc
     3301 cgcctccgga ttgatgctgt acacgcccag ccattcggcc tcacccaata catgaccgcg
     3361 cagcgcctcc tgcagttccg gccagccgaa cacgtccggg aggtccagcc gcggcacgtc
     3421 gagcgcatac acgcggaatc ggtagcggtg cggcaacgcg tcgttccacg gcgggcaggg
     3481 accgtcgtag ccgaagtatt cgccctgcat gtcgggatcg gaagcgaacc agccggtgta
     3541 atcgttgcgc ccctggcgcg agccggcggg cccctcgggt tgccgtttcc cgtggggcgt
     3601 gacgccgtcg ctgcaggcgc ctgccgcaat cgtggcgcac gtggacggga tatccaccat
     3661 cagccagtgg gcgaagtcca cccgcggcag gtcggccggc accgtgcgcc cttccttgtt
     3721 gacgtcgtcg cccctgctgg gcgcatcgcc gtcgatgcat gcgagcgcga acgacttcgc
     3781 cgcggccggc gcgccgctcc acgcgaggtg cggattgcgg ttgcccgcga gcgcgatctt
     3841 ctcgccgccg gttcccggct tgccgaaggc gaactccgcc gggatggtgg cgccgttctg
     3901 gaaactgttg ctgtggatct gcatcgcttc tccctgggtt ctttgcagga tcggcccatg
     3961 gccgcgaaat cgaccggagc aataccacgc gcaacgatcg cgcgcatggc gcgctcctac
     4021 atcatttcgg ttcgtacgta atccggtaga tcactccgtt gccgtcgtcg gagacgagca
     4081 ggctgccgtc gcgcagcggc tgcagcacgg cggccaggac gatggcgcgg caggacgatc
     4141 cggacacgac gatcctcacc cggcacgggt cgtccagcct agccgcagga gtgttacggg
     4201 aaggcgaaaa cgcgaccggg gtcgtgccgc ggtttggcgg tgccccgacc gcaccggccg
     4261 gagcgcttgc ctccctctcc ccgccggaga atcggtcggg atggggggtg ccggagattg
     4321 tcgcgccaag ttctagccgc accgctgccc gcgaccgaac tcagccatcc agcaccgcga
     4381 aggcttcgga aaacacctcc cggtagtggg cggaatggcc gaccgggaag ctgaccggaa
     4441 gtccgccgcg gccccgccgt gcgccgcgcg ccagcggacc gggctcgagg tcgtcgcggt
     4501 gcaggaacac cccgaggcgc aggcgcgtgt cgctggacgc cgccaccagt tggtccggat
     4561 ccacccggaa actcggcagc acgctggcgg cgaactgcag gtgcgacatc cccagtcgca
     4621 accagcgggc agccgcgacg ggttccggca tcgcgaagcc ttccgaccac ccgaagccga
     4681 gccagtcgat gaacatgtcg tgcggttcgc gttgcacgat gacgagccgc gcgcccggca
     4741 gcgcacgccg gatggccggc accacccgcg cgtccagcac cgggatccag tccacgacgc
     4801 gccgcgaatc ctcgacgccc gcgcgctgca gcggacgccg gtagcgccgc gccagcatcg
     4861 cgagctccgg ctggtcgagc ggctgcagca ggctcggttc gaacggcgca ttcacgaaat
     4921 cgggtgcggt ttcgaaacgc tcgcggcgca cgaaccagcc cggctggtcg gcgagcaggg
     4981 ccgcgacctg gcccacgccg gaacctggca ggccgcacag gaacaccggc gcttcgccgc
     5041 gcgcgtcggc cagttccgcc tcgccggcga gccgctgcag gtcctcccga agcgcgtcat
     5101 ccagcagcgg cagcgcgggc aggtcgccgc ggccgaggcg ctgcgctcgc aggaacgcct
     5161 gcgccgcatc cggccattgc tcgagcgcgt cgcaggcgag cccgagcaac cgctgccggc
     5221 ggcgcgcgag ctgcggcggc ttgttcgcga gcgacgcatc gtcgatccgt tgcaggcgct
     5281 ggacggcggc cgcgggatca ccggccgcga gcgccgcgcg cgcccgcaac atcaccagcg
     5341 caggacggtt cgacgacgcc gcggcatcgg ccatggcgag cgcctcgtcg ctgcggcccg
     5401 cccggtcgag ggtcaatgcg tgctgggcct tgagctcgat gtcgtccggc cagcgcgcct
     5461 ccgcctggcc ccacaccgac agcgcttcgt cgtaacggcc ggcccgggtc agcaggtgcg
     5521 ccagttgcgc gtgcgcccgc gggctgtcgg ggtaactcgc cacgtgccag cgcagatcgt
     5581 cgatcgcctg cggcaggcgg ccgcggcgcg ccagggcgtc ggcccgccgg atcgcggcgg
     5641 gatgcaggcc cggctgcacg cgcagcgcgt cgtcgtactg ggcgatcgcc tcgtcgaggc
     5701 ggccctgggc gcgcgcgatg tcgccaatgc cggtgcgcgc cggccccacc tgttccccgc
     5761 gcgccagcaa ggattcatag atcgcgagcg ccggcgcgaa ctcgcgcctg cgcacgtgca
     5821 gctcggcccg cagcgtccgc gcgagtggat agtccggctt gacggtgagc gcgttgtcca
     5881 cggccttggc ggcgaaatcg atcatgccct ggtcgaacat cgcccgggcg tagctggtct
     5941 ggatcaccgg atcggcgggc gcgaggcccg cggcctgggt gagcagttgc agcgcgcgct
     6001 gcggatcgcc gcgcttttgc gcgatgtcgc cgaggccggt cagcgcctgg gcgtcgttct
     6061 cgtcggcccg caacgcgacc ttgaaatggc tttcggccga gtcgagctct ccgcgctgca
     6121 gcgcgacctg gccaaggccg gcgtgggcgg ccgagaggtt ggggttcaac tggcgagcct
     6181 ggtcgtaggc ggcacaggct tcctcgagcc tgccttcggc gaggtacatc tccccgagcg
     6241 tatggcggct ggcggcgttg tgggggtcga gttctccggc gcgctgcacc agcttcttcg
     6301 cctcgggcag gtcgccgcgc tgcccgcgca ggatccccag gaggtgcagc acctcggggt
     6361 tgtccggatt gtcggcgagc aaccggcggt atccgtcttc ggcttcatcg agccgcccgt
     6421 tgcgatgaag ctgcagcagt tcttcgagca tgggtgggtc ctggaacgag cgcgcattat
     6481 gggtgcgtgg ggcggttctg tgcccgcaga gaccaccacg gcgatccatg gccggctttt
     6541 ccacaacagc cgccacgaca gccatgccgg aaggaggatt ttggcattgc gcgccgcgat
     6601 gccgactgcg gacggcgtca gccctccggc gccccaagcg gcttcacgcg agcgcctcca
     6661 cgacggaggc agtgcgggca ccgcccgccg ctgaatggaa ggacgcgcgg gtcgtcgact
     6721 tgcgcgcgcc gttttgggcg cggatggccg ccctacgcga gcgccagccg cgaggcgatg
     6781 aagtcttccg cgaagctgtc gaggtggcga tcgcggatga agccgcagtg gccgccgcgt
     6841 tcggtgaccg tgagcgtgac ggacgcgggc agctccatgt gttccaggtc cgacgcggga
     6901 cacaccggat cgtcgcgcga cgcgaggatg gaggtcggca cggtgagtcc cgcgaggcgg
     6961 tcgccggcca ccgaatagcc gtccagatag ttgtcgaggc tgccgaaatc ggtatgccgg
     7021 aggaccagcg cttcggtcag cgtgcgcagg ctgccgcgca ggtcgtggcg cgtgaacagc
     7081 tcggtttccg aaaacgcgcg ctgcttggcc tgcagcgagc gccgccactt ccacatgaag
     7141 tagtcgtggt acatgcgggg cgcctgctcg agctggaaaa gtcccgaatg cggattgatc
     7201 accgggcaca cggcgaccac atgggcgaga tccacgccgg caccgggtgc gcgcaaggcc
     7261 acccgcaacg cgaagttgcc gcccagcgaa aacccggtca acgtcatcgg gcgcaccggg
     7321 tacaacgact gcagcgcgcg cagcgcaccg acgacctcgt cgatcctgca ggaatggaag
     7381 atcccccggt tcagcgagtg gctcccgccg tggtcgcgga agttcaaccg gaacacgtcc
     7441 cagccctcct ccagcaggcg cgcgccggtc tgcagcaggt aggtcgagcg ggtgctgcct
     7501 tcccagccgt ggaacaacac ggccagtcca cgcggacgcg gcagcttgcg ctgcgccgta
     7561 tggcaaccgg tcaggcggac gccgtcgccg caatcgagcg tcgtccattc ggcgtcacgc
     7621 tccagcgggc gcgagtgccg ccgccgcacg aaccggcgca gcgcactcga tgccaggatc
     7681 gattgcacgt gcgggctctt cagccaccac ggcggccgga agtccgctgc gacgacatcc
     7741 ctcatgtcga caggcccaac acctgcgcga tttccgcgcg cgcggtttcg gccatcctgc
     7801 ggcggccgcc ttcggcatcg aacggcaccg gtttcaggaa gtgcacttcg gcggtcagcg
     7861 acggcaccgc gagcacgcgg aacacgttgt gcaggaaact ctcacccggc gtgaacagga
     7921 cctcgggcga cggccgcccg tcgcgcaggt agcgcagcgc caccggctgc acgggcgcgg
     7981 ccgcatccag cgccgcctgc aggatccgcg catggaacac cttgatggtt cccgccggcg
     8041 cggtgccgcc ttccgggaac gccgcgatgc tgcgcccgtc gcgcaggcgc tcgctcatca
     8101 cggccatcac cgccgtgagc gacccggtgt tgccgcgccg gtggaagatc gtgccgccgc
     8161 gtttggccat ccatccgacc aggggccagt tcccgatctc ggctttggcg acgaagtcgg
     8221 caggccgcac tgcgtgcagc acggtgatgt ccatccacga gccgtggttc gcgacgaaca
     8281 tcaccggatc gtgcaacggt tcgccgtgcc gctgcacgcg caccccggca atgcgcagga
     8341 agatgcgcga ccagttcgtc aacagggagg caccggcggt tccgggtttc gggccgcgcg
     8401 gaagcagggg aatggccgcg aggcacccca cgatcaccac cagcagcaac agcgtcagcc
     8461 gccacgtgca acgccacaca cccaaggtct ctatgggcga cgttgccgcc gcaccctccg
     8521 catctcgaac ggcggctact ttaacgcgtg tccgccgacg cgggcagcgg cgcggccgag
     8581 ggcttacact ggacgcaggc agacgaggtc caggccatga acacgccaca tcgcgtcgaa
     8641 caccagcaca ccgaccgcgg ctacatcccg gtctatgcca cggggatcgt cgaacagccg
     8701 tggaacgact acacgcccac cgaccacgag gtgtgggcca ccctgttccg ccgccagcgc
     8761 gaaatcctga aggaccgcgc ctgccgcgaa ttcctcgaaa accaggagcg cttcgggatg
     8821 tcgcccgacg cgatcccgaa gttcacggat ctgaacgcgg tgctgaaggc cggtaccggt
     8881 tgggagatcg tgggcgtcga ggggctgttg ccggacgagg tgtttttcga ccacctcgcc
     8941 aaccggcgct tcccggtgag ctggtggatc cgcaaacccg agcagctcga ttacctctcc
     9001 gaacccgacc tgttccacga cctgttcggc cacgtgccga tgctgctgaa cccggtgttc
     9061 gccgactacg tgcaggcgta cggccgtggc ggcgtcaagg cgttcaagct cgggcccgat
     9121 gcgctgatga acctgacgcg gctgtactgg tacaccgtcg agttcggcct gatcgacagc
     9181 cccgacggcc tgcgcatcta tggctccggc atcgtcagct ccaagggcga gtcggtgtac
     9241 tgcctcgaat cggaatcgcc gaaccgcatc ggcttcgatc tcgaacgcat catgctcacc
     9301 cgctacaaga tcgacaccta ccagcagacg tatttcgtga tcgacagctt cgagcagttg
     9361 atggacgcga ctcggcccga cttcaccccg atctacgccc ggctcaagga ccagcccgcc
     9421 ttccccgctg gcgacgtcca gcccggcgat cgcgtgatcc atcgcggcac cggcgaaggc
     9481 tggcaaaaaa ccgacgacgt gtaggtcggg ctgatcccgg atgcaaatcc gggagaagcc
     9541 caacgccgta ccgttgcccg ggagcattgg cgctgcttga tcccatgttg ggcttccccc
     9601 ggacggtgtc cggggttcaa cccaacctac gggtgtggtc gcttcactgc gctgacctcc
     9661 agcacctggg cgatcttctc gccgtcccat cggtacatca ggtgcttgcc ttgaacgcag
     9721 ggcctggcga cgtcgggata aaaaagcgct gcgcattcgc cgcccttgca gcgaacgctg
     9781 tcgtacacga tgccgttcga tccttccttt cgaagctccg cgccgagctc gcgcgaggcg
     9841 acgtaactcg tgggatcgtg ctcggccttc cagccgccgc ggatgtcgtg gagcatgccg
     9901 tgcacgcccg tcacgtaggt acgcatggtc acgtcgatcg gcggctcgtg cgtcgcggcg
     9961 aggaaccgct cgcggtggta cacggtctcc tcgaccgcgg tgtcgaattc gtgcgcgaga
    10021 tacaacacgc cgtagctgcc gtcggaaaag cgggtgccct ccgggttgac gtgcgtgaac
    10081 gccgccatca gcggcgtcgt tcccggcccg ctgacgcggt gttccttcgg cacgttggac
    10141 agctcgcccc attcctcgcg caggcgcggg ttcgtcagcg cttcgatccg atagatcgcc
    10201 tcgatgtccg cgccgtccgc gatcgcgtcg aacacgccga ccggcggaaa ccggctcggc
    10261 acgatccggt acgcgcgttg ccagcggagg cggtggaggg gcgggtcgcg tgagggcatt
    10321 ggggaagtat cggggagcag tttctctgac gccgagacga cctggcttcg tgggcactcc
    10381 cgacaccccc aacgaagctg aaagcagacc ctccccctcg tgccgaacgg ctgcagtccg
    10441 gacgcgcggc gatgatgagg gccggaatcg aggggtgcgg gaagctggcg caccctcatc
    10501 cggcgctgcg cgcaccttct ccgaggggag aaggaacaaa ctaccctcgc tgcgcgtcca
    10561 gatactgccg cacgacgtac aggtcggcga cgttgccgga aagcatgcgg tcgagcgcgg
    10621 tcctgccgcc gaaggtcggc gcatcgttgg gactgcgcag ccaggcgtcg gcgcgatccg
    10681 gctgcgggaa caggatagcc agggccttga agatgccgag tacgtagctg atccgttcca
    10741 gcgtatcgcg ggacagggtg ccggcacgct cgcgcttcca cttgtagaag gtcgattccg
    10801 gcgggtcgcc cagcagtttg cgctggtccg ccacgcgcag tttccagcgt tctgcgagcc
    10861 tgaagaaggc tcgcaaggcc gggcccgcaa ggtccgcttc cccctccgtg cggtaaatcc
    10921 cgtcagcgtc ccggatccgc tctgcctgct gtttcatggt agttcctcca tgaagttcac
    10981 ggatacttta cttcatgcag gaagaacaag caatccgtgc acgccgctca ttcgatctgc
    11041 cggtgcggaa cgcctgcctg ggcaagatac gcgtccaacc cgcgttcggg cacgtccgga
    11101 aagacttcgc cggtcgccgt gaaccgcatc cggtcgggac ggaaattgcg gaagtcgccg
    11161 cgcagtcggc accatgcgcc gagcgtccat accggtcccc agcacgccag gcacagcggt
    11221 tcgatctcac gctggctggt ggcaccgtcg gcgtcctggt attccaccct cagcacctgc
    11281 cgtgccgcga tcgcggcatg cagcgcgtcc acgatccccg acttcgcctc gtaccagcgc
    11341 tgcggcacga acacccgcga acgctcggcg cgcccgcgca attccggcgg caacaccgcg
    11401 tcgatcttca gcagcgccga ctgcgccccg cccgccagcc gattgcccgc aaaggcacgc
    11461 acgaagcggg tgccgaccac caacgcctcc agttc
//
LOCUS       DASSNH010000129        18097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_993085, whole genome shotgun sequence.
ACCESSION   DASSNH010000129 DASSNH010000000
VERSION     DASSNH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 18097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18097
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993085"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..1325
                     /gene="zipA"
                     /locus_tag="VFK00_04710"
     CDS             522..1325
                     /gene="zipA"
                     /locus_tag="VFK00_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HET7592288.1"
                     /translation="MEPVSATTLRIIIAAIGVVILVGIYFFGRPRRPGQGRRVVLRKE
                     KDERVEPVLGDAQEGHPVEGGGASPQQGELDVALQRELDRLGAAVAAGRRGATVTEPP
                     ASPEPTVGQRPHEFPVDRIVTLFVIAHEGDVFRGSDIVVAAEKAGLRFGAMHIFHRLV
                     DGRPDAGPVFSMANMIKPGYFNMARIGDLTTPGVTFFITLPGPLAALDAWEAMLPAAQ
                     RIAELLGGELLDEDRNALGRQRVAGLREELRAWDRQHEGPLIGAPPPRR"
     gene            1333..1986
                     /locus_tag="VFK00_04715"
     CDS             1333..1986
                     /locus_tag="VFK00_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HET7592289.1"
                     /translation="MAYPGPAFREDRREVLHAAIEELAFGMMVTHQPDGFATSYLPFE
                     IDAGRGPGGTLIGHLARYNPQSRVSEAGAEALVVFQGPHAYVSPSWYPGKRDDPRQVP
                     TWDYLIVEARGVLTTFDDEARLLNLLNHLTEHHEAARPNRWRVSDAPEDYVHAELRHI
                     IGIELRIESLVGRYKLSQNRKAADQEGARAGLAAASTERERDLAKAIAAAQRERRDR"
     regulatory      2223..2299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2309..3040
                     /locus_tag="VFK00_04720"
     CDS             2309..3040
                     /locus_tag="VFK00_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET7592290.1"
                     /translation="MGDSQKGRHVGRVAGLWRYPVKSMAGEALAEVEVSWHGLAGDRR
                     WAFVRNGLTQSGFPWLTLRERRDMGHYRPSFADSDRPDKSPTMVQTPSGATFDVADPA
                     LGAELCPDGVRVIRQDRGVFDTLPLSLITTQTVARLGETVGTRLEVQRFRPNILVEAA
                     DEAPFAEDAWLGCVLRIGGIRMRVDKRDSRCVVITMDPATLERNPAILRTVARDRQGC
                     LGVYGSTVEPGRIALNDPVLIESRA"
     gene            3134..3541
                     /locus_tag="VFK00_04725"
     CDS             3134..3541
                     /locus_tag="VFK00_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592291.1"
                     /translation="MPTIFRKLNLKDQEEILVLDSPDTFERELEGLRGVHVVRDPGRV
                     KGVAFALAFVSRRDELQRLSTALAAKAVGDAVLWFAYPKGTSKKYTCDFNRDDGWDAL
                     REAGFDSVRQVAIDEDWSALRFRRTEFIKRPRQ"
     gene            3654..4103
                     /locus_tag="VFK00_04730"
     CDS             3654..4103
                     /locus_tag="VFK00_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7592292.1"
                     /translation="MNENLGRDGISQRAAKDIEHVYREWDRAWSTDDLEAMLALYAED
                     ATLESPLIPHLTGGDRGICRGRSEIRKLLEMAAPRKPRKRTFHRKGYFTDGRTLVWEY
                     PRATPDGEQMDFFEVMEVEGGLIRKHRVYWGWRGFEVIKADAYHKET"
     gene            complement(4183..5562)
                     /locus_tag="VFK00_04735"
     CDS             complement(4183..5562)
                     /locus_tag="VFK00_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET7592293.1"
                     /translation="MNRLKFATTIATAALLGGCGGGNAPPEQYGANPPLPAQQRGLLP
                     NMTIAKPAPWGDRLPTVPQGYTVKAIATDLGIPRQSLVLPNGDILVAEGRGGNAPKLK
                     PKDVIAGYVKAKGTSPVEGGNRLTLLRDADGDGTYETKTVFAKDLNAPYGLALVGDTL
                     YVADQDALLKFHYTVGQTQASGAPVKVTDLPSAINHHWTKSLAASADGRYLYVGIGSN
                     SNITERGMTAEVDRARIWQVDVQTGMHRPYATGVRNPTALAIQPGTGTLWAVVNERDE
                     LGPNLVPDYLTAIRDGRFYGWPWSYWGQHVDPRVRPQNPAMVAKAIVPDYSLGSHVAA
                     LGVAFSSPAMGGKFGDGVFVGEHGSWNRADPVGYKVVFVPFRNGRPIGDPIDFVTGFR
                     GDDGLTRGRPVGVSVDPRGALIVADDLSNTIWRVTPSAPSTTVMPPAPAATSPMPPPA
                     ASIAVPARH"
     gene            complement(5559..5984)
                     /locus_tag="VFK00_04740"
     CDS             complement(5559..5984)
                     /locus_tag="VFK00_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592294.1"
                     /translation="MAVVVERTRIALLPINAVLVAGATSLFIGALLGDIAYFRSYAIQ
                     WNNFSSWLLAGALLVAALAFLWSIVELFRTRWRIWPAVAYAVLLLATWILGFIDALVH
                     AKDAWATMPGGLVLSAIVVALACLSLAFAWVGLRTAVVP"
     gene            complement(6160..6420)
                     /locus_tag="VFK00_04745"
     CDS             complement(6160..6420)
                     /locus_tag="VFK00_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HET7592295.1"
                     /translation="MARDLSEDFRQAAEDVQRLPERPDNDTLLKLYALYKQATEGDAN
                     GPKPGFFDFVNTAKYEAWSRLRGMQSDEAQKKYVDLVRQLQT"
     gene            complement(6431..6901)
                     /locus_tag="VFK00_04750"
     CDS             complement(6431..6901)
                     /locus_tag="VFK00_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HET7592296.1"
                     /translation="MTDQPDPLKAAKDAATSGQRFSRGLADSAQQIWLAGLGALARTQ
                     QEGGKFFDALVEEGVRIQEKTHAYNEEQMKQGQQQSTAWMEQARKRTNAAMGKLEKAF
                     DERIARAAKRMRMPSHEDIQELSARIDALTREVRANRAPSAKPRATTKRAKPEG"
     gene            complement(7071..7793)
                     /gene="aat"
                     /locus_tag="VFK00_04755"
     CDS             complement(7071..7793)
                     /gene="aat"
                     /locus_tag="VFK00_04755"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7592297.1"
                     /translation="MIRIRQLDALAPDLFPDPGRALADPNGLLAFGGDLSPQRLVAAY
                     SRGIFPWYSEGDPLLWWSPDPRCVFGTGEVHVSRSLAKFLHKSQWQWGMDRAFEDVMR
                     ACAAPRSGQDGTWITRDMLAAYANLHARGHAHSLEVWEDDELVGGLYGVSIGRMFSAE
                     SMFSRRSNASKTALVALAGVLRPRGFPWIDAQVSNPHLLRLGARMIPRRQFLDELAGL
                     VRQPPPRDWPESLSPVAELSNR"
     gene            complement(7790..8938)
                     /locus_tag="VFK00_04760"
     CDS             complement(7790..8938)
                     /locus_tag="VFK00_04760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592298.1"
                     /translation="MLTLHTHASIDAIAAGDWDALVADSNPFVSHAFLSGLEGTGCIR
                     EEFGWRAQHLTLHDGDALVGALPLYVKANSHGEYVFDWGWADAWERAGGQYYPKFLCA
                     VPYSPVTGPRLLTGAAANAAKRRATLAAALRELTERTGLSSVHVDFLREEETAAFDEA
                     WLARSDWQFQWRNPGGWRDFDDYLGALTHKRRNAIRHERAQVGQGRIDCEFRDGGTLD
                     DAEWRTMHHLYTNTFDEKGNTPTLSLDFFRHLGSAFPEHSHVAFARRRGEIVAGALFL
                     SGKDALYGRYWGAREEVPGMHFELCYYQGIEQCLRRGLARFEPGAQGLHKLARGFLPT
                     RTHSRHYITDDRFREAVRSALRHEAAALEVRGRELRTHSPFAQRDGAP"
     gene            complement(9241..10203)
                     /gene="trxB"
                     /locus_tag="VFK00_04765"
     CDS             complement(9241..10203)
                     /gene="trxB"
                     /locus_tag="VFK00_04765"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7592299.1"
                     /translation="MSTLKHSRLIILGSGPAGYTAAVYAARANLKPTLITGLAQGGQL
                     MTTTDVDNWPGDVEGLQGPALMQRMAEHAERFHTEMVFDHIHSVDLKQRPFRLKGDSG
                     EYSCDALIIATGATAKYLGIASEEKFKGKGVSACATCDGFFFRDQDVAVIGGGNTAVE
                     EALYLANISRKVTLVHRRDKLRAEKIMQDKLFEKVNAGKIEPVWDHTVDEVLGDSSGV
                     SGLRLASTRDGSTREVAVTGMFVAIGHTPNTGVFEGQLDMHNGYIKIKSGLAGNATAT
                     SVPGVFAAGDVADHVYRQAVTSAGFGCMAALDAEKYLDSVGLAG"
     gene            10419..11486
                     /gene="ald"
                     /locus_tag="VFK00_04770"
     CDS             10419..11486
                     /gene="ald"
                     /locus_tag="VFK00_04770"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7592300.1"
                     /translation="MRIGVPSETKTLEGRVALVPAACADLVRHGHEVFLQSGAGGKSG
                     FDDELFTRVGVKVVPDAAALYGSADMIVKVKEPIAGDLDLLEKRHLLFCYLHLAAEPA
                     LTKRLLELGLTGVAFESVQEKDGSLPLLTPMSVVAGKLATQVGTTLLHQPNGGKGKLL
                     GGMAATERGKVVVLGAGVAGGNAAKLAARGGSNVVVFDKRPDRLAQARGWGDNVTALY
                     AYESAVAEEVATADLVVGAVLIPSARAPHVVTRQMVASMEKGSVLVDISIDQGGCFET
                     SKPTTWKDPTYLVDDVTHFCVTNMPGAVPQTSSQAISAAILPYVLRLAQKEWRNDTIL
                     ASGINVANGELVHPALKGMQP"
     gene            11847..14138
                     /locus_tag="VFK00_04775"
     CDS             11847..14138
                     /locus_tag="VFK00_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7592301.1"
                     /translation="MSERARRLLREAAALLLFPLAVYLFACLASYDGTDPGWWSAGVP
                     GRPVHNFGGAVGAYIADLLFHLFGVIAYAFPLLLVVLGVGVLRGWYRRSEGESKWEPS
                     LRLIGFVAFFVGGCGLAWLHASDPQCGSATRAGGLLGCGAGDVLHRGFGELGALLLLL
                     ALLLIAVTWITGLSWFRVMDWTGQQVLTGSGWIRGKLKRAPETLAARAARVERETARK
                     EDLERRSKREPVRIEPAPAPIVKSERAKRETQIPLFTGAASEGEVPPLSLLEEAKPQA
                     GKGYSEETLEVLSRQVEMKLKDFRIEARVVSAHPGPVITRFELEPAPGIRGSQISSLD
                     KDIARGLSLVSVRVVDVIPGKNVIGLEVPNAHREIVYLSEIFSSREYGDARSPLSLAL
                     GKDIGGRPVVVDLQKMPHLLVAGTTGSGKSVALNAMVLSLLFKARPADVRMIMIDPKM
                     LELSVYDRIPHLLAPVVTDMKEAANALRWCVAEMERRYKLMAAVGVRNLAGFNKKVRD
                     AEAAGQPLPDPLFKANPEAPDDKAQPLEPLPYIVVVIDEFADMMMIVGKKVEELIARL
                     AQKARAAGVHLILATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTILDQSGAETLL
                     GNGDMLYLPPGTATPERVHGAFVGDDDVHKVVEWLRTQGEPRYIEGVLEDVQAMGDGK
                     IINESGLPEDADGDGDGDTLLYDKAVAIVTSSRRASISGVQRHLRIGYNRAARLIEQM
                     EADGVVSAPQHNGTREVLAPPPA"
     gene            14377..15021
                     /gene="lolA"
                     /locus_tag="VFK00_04780"
     CDS             14377..15021
                     /gene="lolA"
                     /locus_tag="VFK00_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HET7592302.1"
                     /translation="MRKLILLFGLSLVAGGALAATGARARLDAFADGLHSLQGNFKQT
                     VFDAHGNITGSSRGTLALEAPRLFRWNVTEPYQQLMVADGHKVWVYEPDLEQVTVRDQ
                     GAEEAHSPLTVLTDLSQLDTEFKASDAGTHDGLEWLRLVSRAKEPQFEYAEIGFDATG
                     LRRMVFKDTLGNRTEIAFSDWQRNVGLPADTFTFVPPKGTDVVGDAAGGSGQPR"
     gene            complement(15031..16761)
                     /gene="sulP"
                     /locus_tag="VFK00_04785"
     CDS             complement(15031..16761)
                     /gene="sulP"
                     /locus_tag="VFK00_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HET7592303.1"
                     /translation="MSTSQPAAASSLQLYVPKLVTLLRRGYHFDDFRHDLVAGLTVAV
                     VALPLAMALAIASGTTPEKGLYTAIVAGFLISLLGGSRVQVGGPTAAFIPVVFNVIDK
                     HGFGGLILCTLLAGCMLIAAGVLRLGVLLKYIPKPVVTGFTAGIAVSIFSSQVKDLLG
                     LDMARVPAEFFARWSAYAQHLPGFSAATVAVSVACVAVILLLRKYRPNWPGFLIVVVG
                     GAAVVTLIGLPVDTIGTRFGGIPDALPTFSFPHIPFERTRELLPVSFTIAFLAGVESL
                     LSAVVADGMIGGRHRSNCELVAQGVANIGSALFGGLPATGAIARTATNVRSGARTPIA
                     GMLHAAFLLVFMLVLAPLIQYVPLATLAAILVVVAWNMAEIESFRHLMSGPIGDRAVL
                     LLTFVLTVVFDLTVAIQVGVVLAAFLFMHRMAGVVAMESHVTLIDDDQADTIHPTAPD
                     QRAQLPPGVEAFRISGPLFFAVANRLDDVLNQFFKPPRVFILRMSQVPLIDSSGVTAL
                     GQMLGRCRRTGIRVIFSGLQEQPRAILADMGIREDGVHLRFARDFAEAVRWAAAGSQE
                     GGQPAETTRT"
     gene            complement(16867..>18097)
                     /locus_tag="VFK00_04790"
     CDS             complement(16867..>18097)
                     /locus_tag="VFK00_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7592304.1"
                     /translation="PADPRDPYADYDAAKLKAFLAGYKLPRDPGAKYEYSNLGLGLLG
                     YALARHAGTSYPQLLQSRVFGPLGMTSTSATLKEPLAPRWAPGHDEQGKPAQPWHLDA
                     LAGAGAINSTGTDMLRYLEANMGRGEGALRRAMKFAHEPRRAVGGDERIGLAWMTKRG
                     KSGDVVWHNGMTGGYASFLGFTADGTRGVMILTNIQQSVDDLGFAALLPDAPLAPARK
                     QIALAPKQLQDYIGEYRLAPDFVLTVMQQGDQLLAQATGQGAFPIFPDATDKFFAKIT
                     DIRIDFKRDGKGKVESLVLHQNGHDMPAPKLDAAESAELGGHKTVRLDTATLKQYVGR
                     YQLAPGAVFDITLDHGQLKAQLTGQPAAPVYPSAKDEFYYTVVDARLSFKRGADGKVD
                     ALVLHQNGTDRAAKRIP"
BASE COUNT         2948 a         5875 c         6260 g         3014 t
ORIGIN      
        1 tcgtgcgcga catgtccgaa aaggtccagt tcatggtcgt gacccacaac aaggcgacca
       61 tggaaatggc ctaccagttg tgcggcgtca ccatgcgcga acccggcgta tcgcgcctgg
      121 ttcaggtcga cctcgccgag gccgcaaagc tcgcaggcct cgccgcttaa tcgtagggct
      181 ttgctgcgtt ttgtaggttg ggttgatccc ggacaaggtc cgggagaagc ccaacagccg
      241 cgacatggat cgaagatcgt tgggcttcgc tgcgctcagc gccaacctac aagagcgtcc
      301 gtcgcagcca cggttcaggc cgccgagcac gtaggttggg ttgatcccgg acaaggtccg
      361 ggagaagccc aacggccgcc gcatggatcg aagatcgttg ggcttccccc ggacggagtc
      421 gggggtcagc accatcctgc aagagcctcc ggcgcacccg ttttttccca cgcgcacagc
      481 catggttttt ggccgtcgag ttaactgcta gagtccgccc catggaaccg gtttccgcca
      541 cgaccttgcg catcatcatt gccgcgatcg gcgtcgtgat cctggtcggc atctattttt
      601 tcgggcgccc gcggcgtccc ggacaaggtc ggcgcgtcgt gctccgcaag gagaaggacg
      661 agcgcgtcga gccggtcctg ggcgatgcgc aggaagggca tccggtcgag ggcggtggcg
      721 cctccccgca acaaggcgag ctcgacgtgg cgttgcaacg cgagctggat cgcctcggcg
      781 cggcggtcgc ggccggacga cggggtgcga cggtaaccga gccgcccgcg tcaccggaac
      841 ccacggtagg gcaacgaccc cacgagtttc cggtggaccg catcgtcacc ctgttcgtga
      901 tcgcgcatga gggcgacgtg ttccgcggca gcgacattgt ggtcgccgcg gagaaggcag
      961 ggctccgatt cggcgcgatg cacatcttcc atcgcctggt cgatgggcgt cccgacgcgg
     1021 gcccggtgtt cagcatggcc aacatgatca agcccggcta tttcaacatg gcgcggatcg
     1081 gcgatctgac cacgccgggc gtgacgttct tcatcaccct gccgggaccg ctcgccgcgc
     1141 tggacgcgtg ggaagccatg cttccggcgg cccagcggat cgcggaactc ctgggcggcg
     1201 aattgctgga cgaggaccgc aatgcgctgg gtcgccagcg cgtcgccggc ttgcgcgaag
     1261 agcttcgcgc atgggaccgg cagcacgaag ggccgctgat cggcgcgccg ccgccgcgac
     1321 gctgatacgt agatggcgta tccgggtccg gcattccgcg aagaccgcag ggaagtcctg
     1381 cacgctgcca tcgaagaact cgccttcggc atgatggtca cgcaccagcc ggacggtttt
     1441 gcaacctcct acctgccgtt cgagatcgat gccgggcgcg gtcccggggg cacgctgatc
     1501 gggcacctcg cgcgctacaa cccccagtcg cgggtttccg aagcgggcgc cgaggccttg
     1561 gtcgttttcc aggggccgca cgcctacgtg tcgccttcct ggtatcccgg caagcgcgac
     1621 gatccgcggc aggtaccgac gtgggactac ctcattgtcg aagcgcgcgg cgtgctgacg
     1681 acattcgacg acgaggcgag gctgctgaat ctgctcaacc acctgaccga gcaccacgaa
     1741 gcagcgcgcc cgaaccggtg gcgcgtgagc gatgcccctg aagattacgt gcacgccgaa
     1801 ttgcgccaca tcatcggcat cgaattgcga atcgaatcgc tggtcggccg ctacaagctg
     1861 agccagaacc gcaaagccgc cgaccaggaa ggcgcacgcg cgggactcgc cgccgcatcg
     1921 accgaacgcg agcgcgactt ggcgaaagcc atcgcagcgg cacaacgcga gcggcgcgat
     1981 cgctgagcgc aagcacctgc gtgcaccgct catcgagctc gttgtgcgcc agcgggtgta
     2041 gctgcttacc tcggcaggtt caacgcaacg agagtccaca tggactacgg acgactggct
     2101 tgtgctcgcg gtcctggtgc ttgcatgggg tggttcgcgc cgcctgcaac gagttgacga
     2161 ctgccggccc ccatggtttc ctgcgtgcga ctgtctccgt tgcgctgcct cgccagcgca
     2221 ccgtcgccga ggggcgctgc agcggatcgg aatccgccac gctcggcgac ggttggtcgg
     2281 ccaagggcgc ctctcgggag gctgcgccat gggtgattct caaaagggca ggcacgtcgg
     2341 gcgcgtcgcg gggttgtggc gctacccggt caagtcgatg gctggcgaag cgctggccga
     2401 ggtcgaggtg tcctggcacg gccttgcagg cgatcgtcgc tgggcgttcg tccggaacgg
     2461 gctgacgcag agcggatttc cctggctcac gctgcgcgag cgcagggaca tgggccatta
     2521 ccgcccgtcg tttgccgatt cggatcgccc cgacaaatcg ccgacgatgg tgcagacgcc
     2581 gtcgggcgcg accttcgatg tcgcggatcc ggcgctgggg gcggagcttt gccccgacgg
     2641 cgtgcgcgtc attcggcagg accgcggcgt cttcgatacc ttgccgctgt ccctcatcac
     2701 gacgcagacg gtcgcgcggc tgggtgaaac cgtcggcacc cgcctcgaag tgcagcgctt
     2761 ccggccgaac atcctggtgg aggctgccga cgaggcgcct ttcgccgagg atgcctggct
     2821 gggttgcgtc ctgcgcattg gcggcatccg catgcgtgtc gacaagcgcg acagccgttg
     2881 cgtggtcatc acgatggatc cggcgacgct ggagcgcaat ccggcgatcc tgcgcacggt
     2941 ggcgcgcgat cgccagggtt gcctcggcgt gtatggatcg accgtggaac caggtcggat
     3001 tgcattgaac gaccccgtgc tgatcgagtc gcgtgcctga gcctgctgct gctgccgatt
     3061 tgccggaagc cgccggcccg gtcgatcaga tcgcagctac gatcgcattt ccgccgcgcc
     3121 gcgtgtgagc cccatgccca cgatcttccg gaagttgaac ctgaaggacc aggaagaaat
     3181 cctggtcctg gattcgcccg atactttcga gcgggagctc gaaggccttc ggggtgtcca
     3241 tgtcgtccgg gatcccggca gggtaaaggg cgttgcgttc gcgctggcct tcgtgagccg
     3301 ccgggacgaa ctccagcgac tgtcgaccgc gctcgccgcc aaagcggtgg gcgacgcggt
     3361 gttgtggttc gcctacccga agggcacctc gaagaaatac acctgcgact tcaaccggga
     3421 cgacggctgg gacgcgctgc gcgaagccgg attcgacagc gtccgccaag tcgcgatcga
     3481 cgaggactgg tccgcgctcc gtttcaggcg caccgaattc atcaagcggc ccaggcaata
     3541 gctgcaagcc acgtgtcgcc ggtgggcccc gatcgacgca ttcgcggtag gcctcggttt
     3601 aacagacacg atctaacctg tcgcggtgca cccacgcgat cgcaggaaaa gggatgaacg
     3661 aaaatctggg gcgagacggc atctcccaac gcgcggcgaa agacatcgag catgtctatc
     3721 gcgaatggga ccgggcgtgg tcgacggacg acctggaagc gatgcttgcg ctttacgcag
     3781 aagatgcaac gctggaaagc ccgctgatcc cgcacttgac cggtggcgac cggggcattt
     3841 gtcgcgggcg cagtgaaatc cgcaagctgc tggaaatggc cgcgccgcgg aagccgcgca
     3901 agcgcacgtt ccatcgcaag ggctacttca ccgacggccg aacgctcgtg tgggagtatc
     3961 cgcgtgccac gccggacggt gaacagatgg atttcttcga ggtcatggaa gtggagggtg
     4021 gcctcatccg gaagcaccgt gtttattggg ggtggcgcgg cttcgaggtc atcaaggccg
     4081 acgcgtacca caaggagacg tgagccggtc cagcaccacg cgcgacgccg gagcccgcag
     4141 tcggcgttgg atttcctgct gctccttggc gcggctcgga attcagtgcc tggcaggcac
     4201 cgcaatggaa gcggctggcg gcggcatggg tgatgtcgcg gccggcgccg ggggcatcac
     4261 cgtcgtggac ggtgcgctgg gcgtcacgcg ccagatcgtg ttcgacaggt cgtcggcgac
     4321 gatcagggcg ccgcgcggat cgacgctcac gcccaccggt cgtccgcggg tcaaaccgtc
     4381 gtcgccgcgg aagccggtga cgaagtcgat cggatcgccg atgggcctgc cgttgcgaaa
     4441 cggcacgaat accaccttgt aacccactgg atcagcacgg ttccaactgc cgtgttcgcc
     4501 gacgaatacg ccgtccccga acttgccgcc cattgcgggc gacgagaacg cgacgccgag
     4561 ggcggccacg tgcgagccga ggctgtagtc gggcacgatg gccttggcga ccattgccgg
     4621 gttctgtgga cgcacgcgcg gatccacgtg ctggccccag tagctccacg gccagccgta
     4681 gaacctgccg tcgcgtatcg cggtgaggta gtcgggcacg aggttgggac cgagttcgtc
     4741 gcgctcgttg accaccgccc acaaggtgcc ggtgccgggc tggatcgcga gcgcggtcgg
     4801 attgcggacg ccagttgcgt agggccggtg catgccggtc tggacgtcca cctgccagat
     4861 ccgagcgcga tcgacctcgg cggtcatgcc gcgctcggta atgttgctgt tcgagccgat
     4921 gccgacgtac aggtagcgcc cgtcggcact ggcagcgaga gacttggtcc agtgatggtt
     4981 gatcgccgac ggcaggtcgg tgaccttgac cggcgcgccg ctcgcctggg tctggccgac
     5041 ggtgtagtgg aatttcagca gcgcgtcctg gtcggcgacg tagagcgtgt cgcccaccag
     5101 ggcgagtccg tagggtgcgt tgagatcctt cgcgaacacg gtcttcgtct cgtacgtgcc
     5161 gtcgccgtcc gcatcccgca gcagggtcag gcggttgccg ccctcgaccg gactcgtgcc
     5221 cttggccttc acgtaaccgg cgattacatc tttcggtttc agcttcggtg cgttgccgcc
     5281 gcggccttcg gccaccagga tgtcgccgtt gggcagcacc agcgactggc gcgggatgcc
     5341 gagatcggtg gcgatcgcct tgacggtata gccctgcggc acggtcggca gccggtcgcc
     5401 ccaaggcgcc ggcttggcga tcgtcatgtt cggaaggagg ccgcgctgct gtgccggcag
     5461 cggcggattg gcgccgtatt gttcgggggg cgcgttgccg ccgccgcaac cgccgagcag
     5521 ggccgcggtg gcgatggttg tcgcgaactt caggcgattc atggcacgac tgccgtgcgc
     5581 agcccgaccc aggcgaatgc cagcgacagg cacgccagcg ccaccacgat cgccgacagc
     5641 accaggccgc ccggcatcgt tgcccaggcg tccttggcgt gcaccagcgc gtcgatgaag
     5701 ccgaggatcc aggtcgcgag cagcaacacc gcgtaggcca cggcgggcca aatccgccag
     5761 cgggtgcgga acagctcgac gatggaccag aggaatgcca gtgcggcgac gagaagggcg
     5821 ccggcgagca gccacgacga gaagttgttc cactggattg cgtagctgcg gaaataggcg
     5881 atgtcgccca gcagcgcgcc aatgaacagc gacgtggccc cggccacgag tacggcgttg
     5941 atggggagaa gtgcgatccg cgtccgttcc accacgacag ccatgacggc gtcctcctga
     6001 cggctcgtgc cacgacgcaa cgtcagggta caacagcgtc gtgatttccg ggtgacgggt
     6061 cgcggcagcg ttggcgatcg gcggtttccc gccggtgaca ccccgttcca cgtcgggctt
     6121 tcccggacgg cagtccgggg tcggcgccaa cctgcgggct taggtctgga gttgccgcac
     6181 caggtcgacg tatttctttt gcgcctcgtc gctttgcatc ccgcgcagtc gtgaccaggc
     6241 ttcgtacttc gcggtgttga cgaaatcgaa gaagcctggc ttcgggccgt tcgcgtcgcc
     6301 ttcggtggcc tgcttgtaga gcgcgtagag cttcagcagg gtgtcgttgt ccgggcgctc
     6361 cggcaggcgc tggacgtcct cggcggcctg gcggaaatcc tcgctgaggt cgcgggccat
     6421 ggcgtttcgc tcagccttcc ggcttggcgc gcttggtcgt ggccctcggt ttcgcggaag
     6481 gcgcgcggtt cgcacggacc tccctggtca gcgcgtcgat gcgcgcggac agttcctgga
     6541 tgtcctcgtg gctcggcatt cgcatgcgct tcgccgcgcg ggcgatccgt tcgtcgaacg
     6601 ccttctcgag cttgcccatc gccgcattgg tgcgcttgcg cgcctgctcc atccacgcgg
     6661 tcgactgttg ctgcccctgt ttcatctgtt cctcgttgta ggcgtgcgtc ttctcctgga
     6721 tgcgcacgcc ttcctccacc agcgcgtcga agaacttgcc gccttcctgc tgggtgcgcg
     6781 cgagtgcgcc gaggccggcg agccagatct gctgcgcgga atcggcgagg cctcggctga
     6841 agcgctgccc ggacgtcgcg gcgtccttgg cggccttgag gggatcgggt tggtcggtca
     6901 tggcggcggc tccggatggc agggcaaaca atgtacgcca actcgcgttc aaattcaggc
     6961 aaggccggca gctcgtggcg cggccactgt ccgccgccgc gtttgatcgg gacggtgccc
     7021 gccctgcgac acgcgccctg ccgaccggcc gcttggcttg cgatggcttt tcatcgattc
     7081 gatagctcgg ccaccggcga cagcgactcc ggccagtcgc gtggcggcgg ttgccggacc
     7141 agccccgcca actcgtccag gaattgccgg cgcgggatca tgcgcgcgcc cagccgcagc
     7201 aggtgcgggt tcgacacctg cgcgtcgatc cacggaaagc cgcgcgggcg gagcacgccg
     7261 gcgagcgcga caagcgcggt cttggaggcg ttgctgcggc gcgagaacat cgactcggcg
     7321 ctgaacatgc gcccgatcga cacgccgtag agtccgccga caagttcgtc gtcctcccag
     7381 acctccagcg agtgggcatg gccgcgtgca tgaaggttgg cgtaggcggc gagcatgtcg
     7441 cgggtgatcc acgtcccgtc ctgcccgctc cggggcgcgg cgcaggcccg catcacgtcc
     7501 tcgaaggcgc gatccatgcc ccactgccat tgcgacttgt gcaggaactt ggcgaggctc
     7561 cgcgacacgt gcacctcgcc ggtcccgaat acgcagcgcg gatccggtga ccaccacagc
     7621 agcgggtcgc cttccgaata ccacgggaag atcccgcgcg agtaggcggc gaccaaccgt
     7681 tgcggcgaca ggtcgccgcc gaacgccaac aggccgttgg gatcggcgag cgcgcggccg
     7741 gggtccggaa acaggtcggg cgccagcgca tcgagctgcc ggatccggat catggcgccc
     7801 catcgcgttg cgcgaatggt gaatgcgtcc gcaattcgcg gccgcgcacc tcgagcgccg
     7861 ccgcctcgtg ccgcaaggcg ctgcgcacgg cttcgcggaa tcggtcgtcg gtgatgtagt
     7921 gccgggaatg ggtgcgggtc ggcaggaatc cgcgtgcgag cttgtgcagg ccctgcgcgc
     7981 cgggttcgaa gcgagcgaga ccgcgccgca ggcattgctc gatgccttgg tagtagcaca
     8041 gctcgaagtg catgcccggc acttcctccc gcgcacccca ataacggccg taaagagcgt
     8101 ccttcccgga caggaacagt gcgcccgcca cgatctcgcc ccggcgtcgc gcgaacgcga
     8161 cgtgcgaatg ttcgggaaat gcggagccga gatggcggaa gaagtcgagc gacaaggtcg
     8221 gcgtgttgcc cttttcgtcg aaggtgttgg tgtacagatg gtgcatggtc cgccattcgg
     8281 cgtcgtccag cgtgccgccg tcgcggaatt cgcagtcgat cctgccctgg cccacttgcg
     8341 cgcgttcgtg gcggatcgca tttcgacgct tgtgcgtcaa tgcgccgagg tagtcgtcga
     8401 agtcgcgcca gccgccggga ttgcgccact ggaattgcca atcggagcgc gcgagccacg
     8461 cctcgtcgaa ggccgcggtt tcctcttcgc ggaggaaatc gacgtgcacc gatgaaaggc
     8521 cggtgcgttc ggtcagttcg cgcaatgcgg ccgccagcgt ggcgcgacgc tttgcggcat
     8581 tcgccgcggc gcccgtgaga aggcgtggcc ccgtgacagg cgagtagggc accgcgcaca
     8641 ggaacttcgg gtagtactgg ccgccggcac gttcccaggc gtcggcccag ccccagtcga
     8701 acacgtattc gccgtgcgag ttggccttga cgtacagcgg caaagccccg accagcgcgt
     8761 cgccgtcgtg gagcgtcagg tgttgcgcgc gccagccgaa ttcctcgcgg atgcaaccgg
     8821 tgccctcgag accggacagg aacgcgtggc tcacgaacgg attcgaatcg gcgaccagcg
     8881 catcccaatc gcccgccgcg atggcatcga tcgacgcgtg ggtgtgcagc gtgagcatgc
     8941 gtctattctg gcagggggtg atctgaagat cgcccgaatc acgcagaccg acaaaagcat
     9001 tgggcgcgga tcaacgcgga taggacctta tccaacagaa gtgaaaccgt tcgtgccgag
     9061 cgtagctccg cgaagtcgaa acacccggat tccgcaacgg tgtcacttcg acttcgcgcc
     9121 tttcggcgct tccctcagtg cgaccgaccc tacggaccaa ttccaaaggc agatggctct
     9181 atccgccttt atccgcgtca aaaagcttat gcgcgcgtcg tgcgcagaac gggcgagcga
     9241 tcagcccgcc agcccgaccg aatcgagata cttctcggca tccagcgcgg ccatgcatcc
     9301 gaagcctgcc gaagtgaccg cctggcggta cacgtggtcg gcgacgtcgc ccgccgcgaa
     9361 cacgcccggc accgaggtgg cggtggcatt gccggccaac ccgctcttga tcttgatgta
     9421 gccgttgtgc atgtcgagct ggccttcgaa tacgccggtg ttgggcgtgt ggccgatcgc
     9481 gacgaacatg ccggtgaccg cgacttcgcg cgtgctgccg tccctggtgc ttgcgaggcg
     9541 gagcccgctg acgccgctgc tgtcgccgag cacctcgtct accgtgtggt cccacaccgg
     9601 ctcgatcttg ccggcgttga ccttctcgaa cagcttgtcc tgcatgatct tttccgcgcg
     9661 cagcttgtcg cggcggtgca ccagtgtcac cttgcgcgag atgttggcca ggtacagcgc
     9721 ttcctcgacc gcggtgttgc cgccgccgat cacggcgacg tcctggtcgc ggaaaaagaa
     9781 tccgtcgcag gtggcgcacg ccgacacgcc cttgcccttg aacttctcct cgctggcgat
     9841 gccgaggtac ttggcggtgg cgccggtggc gatgatcagt gcgtcgcaac tgtattcacc
     9901 gctgtcgccc ttcaaccgga acgggcgctg cttcaggtcg acgctgtgga tgtggtcgaa
     9961 caccatctcc gtgtggaatc gctcggcgtg ctcggccatg cgctgcatca gcgcgggacc
    10021 ctgcaggcct tccacgtcgc cgggccagtt gtcgacgtcg gtcgtggtca tcaactggcc
    10081 gccctgggca aggccggtga tcagcgtcgg cttcaagttc gcgcgcgccg catagacggc
    10141 cgcggtgtac ccggccggac ccgagcccag gatgatcagg cgactgtgtt tcagggtgct
    10201 catgtatact tccgaagttg ttttcgaggt ccgcagaact tccccaccgc ttgccgtggc
    10261 gcgtttccag gtgccgcgac cccgatcgct gtcttgcccg tgacacccgc gcgtcgcgcg
    10321 cccggtctca cgtgcaaccg gtggcgagtc gtgcagcatg gggagtttgt ggcggtattt
    10381 caagcgcgtg aaagttgatt ttcgcagagg atgacgagat gcgtattggc gttccttccg
    10441 aaaccaagac cctcgagggt cgggtcgccc tggttccggc ggcatgtgcc gaccttgttc
    10501 ggcatggcca cgaagtgttc ctgcagtccg gtgcgggcgg caagagcggt ttcgacgacg
    10561 agcttttcac ccgtgtcggc gtgaaggtgg tgcccgacgc cgccgcgctg tacggatcgg
    10621 cggacatgat cgtgaaggtc aaggagccga tcgccggcga cctcgacctg ctcgagaagc
    10681 ggcacctgct gttctgctac ctgcacctcg ccgccgaacc cgccctgacc aagcgcctgc
    10741 tcgaacttgg cctgaccggc gtcgcattcg aaagcgtgca ggaaaaggat ggttccttgc
    10801 cgctgctgac cccgatgtcg gtggtcgcgg gcaaactggc cacccaggtc ggaaccacgc
    10861 tgctgcatca gccgaacggt ggcaagggca agctgttggg cgggatggca gcgaccgagc
    10921 gcggcaaggt cgtggtgctg ggcgccggcg ttgccggtgg caacgcggcg aagctcgcgg
    10981 cccggggcgg aagcaacgtg gtggtgttcg acaagcggcc ggatcgtctg gcgcaggcac
    11041 gcggctgggg cgacaacgtc accgcgctgt atgcctacga gtcggcggtc gcggaggagg
    11101 tcgcgacggc cgaccttgtg gtgggcgccg tgctgattcc gagcgcccgg gcgccgcacg
    11161 tcgtgacccg ccaaatggtc gcgtcgatgg aaaagggcag cgtgctggtc gacatctcga
    11221 tcgaccaggg cggctgcttc gagacctcga agccgaccac ctggaaggat ccaacgtatc
    11281 tggtcgatga tgtcacgcac ttctgcgtca ccaacatgcc cggcgcggtc ccgcagacgt
    11341 cctcgcaggc gatctcggcg gcgatcctgc cgtacgtgct gcgcctcgcg cagaaggaat
    11401 ggcggaacga cacgatcctc gccagcggca tcaacgtcgc gaacggcgaa ctggtgcatc
    11461 cggcgctgaa aggcatgcag ccctgacggt gtttcctgcc cccttcgcgc gcgaaagggg
    11521 gcgggcgccg caggcccgcg ggggaatggc cgttgctctt tgggcttgcc cgatgcagcc
    11581 cggccagccc ttcctgcaaa ctctttccgt ccgggcgcgc gcgtggcgcg ctcatgcacg
    11641 ggggcaaaac aggcggtgcc ggcgcgggca agaccgtgcc aaaaacgcgc aaaacgtgag
    11701 cgcgtcgaca gcgcgggacg ccggccggtg ccggggcttg gtccccgccg aaacggcggt
    11761 atagtcagcg cgcaaaaggt catccggaat cgaggttgcc acgcgtggcg cgtgcgagag
    11821 tacaggtcca ggccaaatcc cccggaatga gcgaacgcgc gcggcggttg ctgcgcgagg
    11881 cggcggcgtt gctgctgttc ccgctcgcgg tgtacctgtt cgcgtgcctc gcgagctacg
    11941 acggcacgga tcccggctgg tggagcgccg gcgtgccggg gagaccggtg cacaatttcg
    12001 gcggcgccgt gggcgcctac atcgccgacc tgctgttcca cctgttcggc gtcatcgcct
    12061 acgcgtttcc cttgctgctg gtggtgctcg gcgtcggcgt gttgcgcggc tggtaccggc
    12121 gcagcgaagg cgagagcaag tgggaaccga gcctgcgcct gatcggattc gtggcgttct
    12181 tcgtcggtgg ctgcggactc gcctggttgc acgcgtcgga tccccagtgc ggcagcgcca
    12241 cgcgcgcggg cgggctgctc ggttgcggcg cgggcgacgt gctgcatcgc ggcttcggcg
    12301 aactcggggc gctgttgctg ctgctggcgt tgctgctgat cgcggtcacc tggatcaccg
    12361 ggctttcgtg gttccgggtg atggactgga ccgggcagca ggtgctgacc ggctcgggct
    12421 ggattcgcgg caagctcaag cgggcgccgg aaacgctcgc ggcgcgcgcg gcgagggtcg
    12481 agcgcgagac ggcgcgcaag gaagacctcg aacgccgctc gaagcgggag cccgtgcgga
    12541 tcgagcccgc gcccgcgccg atcgtcaaga gcgagcgggc caagcgcgag acccagattc
    12601 cgctgttcac cggcgccgcg agcgaaggcg aggtgccgcc gctgtcgctg ctcgaagaag
    12661 ccaagccaca ggccgggaag ggttattctg aagagaccct cgaggtgctt tcgcgccagg
    12721 tcgagatgaa gctcaaggat ttccggatcg aagcacgggt ggtcagcgcg catccggggc
    12781 cggtgatcac gcggttcgag ctggagcccg cgcccggcat ccgcggcagc cagatttcct
    12841 cgctcgacaa ggacatcgcg cgcggcctgt cgctggtcag cgtgcgcgtg gtcgacgtga
    12901 tcccgggcaa gaacgtgatc gggctcgagg tgccgaatgc gcatcgcgag atcgtgtacc
    12961 tgtcggaaat cttcagctcg cgcgaatacg gggacgcgcg ttcgccgctg tcgctcgcgc
    13021 tcggcaagga catcggcgga cgcccggtgg tcgtggacct ccagaagatg ccgcacctgc
    13081 tggtcgccgg caccacgggt tccggcaagt cggtggcgct gaacgcaatg gtgctgtcgc
    13141 tgttgttcaa ggcgcggccc gcggacgtcc ggatgatcat gatcgacccc aagatgctgg
    13201 agctgtcggt gtacgacagg atcccgcacc tgctggcgcc ggtcgtgacc gacatgaagg
    13261 aagccgccaa cgcgttgcgc tggtgcgtgg ccgaaatgga acgccgctac aagctgatgg
    13321 ccgcggtggg cgtgcgcaac ctcgccggtt tcaacaagaa ggtgcgcgac gccgaggccg
    13381 cggggcagcc gctcccggat ccgctgttca aggccaatcc cgaggcgccc gacgacaagg
    13441 cgcagccgct ggaaccgctg ccgtacatcg tggtggtgat cgacgaattc gccgacatga
    13501 tgatgatcgt cggcaagaag gtcgaggaac tgatcgcgcg gctcgcccag aaggcgcgcg
    13561 ccgcgggcgt gcacctgatc ctcgcgaccc agcggccgtc ggtggacgtg atcaccgggc
    13621 tgatcaaggc caacatcccg acccgcatcg cgttccaggt ttcctcgaag atcgattcgc
    13681 gcacgatcct cgaccagtcc ggtgcggaaa ccctgctcgg caacggcgac atgctgtacc
    13741 tgccgccggg taccgcgacg ccggagcgcg tccacggcgc gttcgtcggc gacgacgacg
    13801 tgcacaaggt cgtggaatgg ctgcgcaccc agggcgagcc gcgttacatc gaaggggtgc
    13861 tggaagacgt gcaggcgatg ggcgacggca agatcatcaa cgagtcgggg ttgcccgaag
    13921 acgcggacgg cgacggggac ggcgacaccc tgctgtacga caaggccgtg gcgatcgtca
    13981 cgtcctcgcg gagggcgtcg atctcgggcg tgcagcgcca cctgcggatc ggctacaacc
    14041 gcgccgcgcg cctgatcgag cagatggaag ccgacggcgt cgtgtcggcg ccgcagcaca
    14101 acggcacccg cgaggtactc gctccgccgc cggcatagtt tttgcgatcg tccctgatcg
    14161 cggcatcgcc actcccacaa ccccgccgga aggcttcggg ccatggagtc cagaacggcc
    14221 atccctggcc cgactttcca cgacagccgc ttcgctagag gcgcacggga gggtggttgt
    14281 cggggaacat gctctcccgc tggttagctc gttctcatgc gccattcacg agcttccggc
    14341 acaatgcgcc ccgtgcgatc gccccggaaa acgcccatgc ggaagttgat cctgttgttc
    14401 ggcctgtcgc tggtcgccgg cggcgcgtta gcggccacag gcgcgcgcgc gcggctcgat
    14461 gcattcgccg atggcctgca ttcgctgcaa ggcaatttca agcagacggt gttcgacgcc
    14521 cacggcaaca tcaccggctc cagccgcggc acgctggcgc tggaggcgcc gcggctgttc
    14581 cgctggaacg tcaccgaacc gtaccagcag ttgatggtgg ccgatggcca caaggtgtgg
    14641 gtgtacgagc ccgacctgga acaggtcacg gtgcgcgacc agggcgccga ggaagcgcac
    14701 agtccgctga cagtgctgac cgacctgtcg cagctcgaca ccgaattcaa ggcgagcgac
    14761 gccggcacgc acgacggtct ggagtggctg cggctggtgt cgcgggcgaa agagccgcag
    14821 ttcgaatacg ccgagatcgg tttcgacgcc accggcctgc gccggatggt gttcaaggac
    14881 accctcggca accgcaccga aatcgcattc tccgactggc agcgcaacgt cggcctgccg
    14941 gcggatacct tcaccttcgt gccgcccaag ggcacggacg tggtgggcga tgccgcaggc
    15001 gggagcggcc agccgcgctg aagcgggcgg tcaggtgcgg gtggtttccg caggctggcc
    15061 gccttcctgt gagcctgccg ccgcccatcg caccgcttcg gcgaaatccc gggcaaagcg
    15121 caggtgcacg ccgtcttccc ggatgcccat gtcggcgagt atcgcgcgcg gttgctcctg
    15181 caagcccgag aagatgaccc ggattccggt gcggcggcaa cgtcccagca tttgacccag
    15241 cgcagtgacg ccactgctgt cgatcagcgg gacctggctc atgcgcagga tgaagacgcg
    15301 cggcggcttg aagaactggt tcagtacgtc gtcgaggcgg ttggcgaccg cgaagaacag
    15361 cgggccggaa atccggaacg cctccacgcc gggtggcaat tgcgcgcgct ggtcgggagc
    15421 ggtggggtgg atggtgtcgg cctggtcgtc gtcgatcagc gtcacgtggg actccatcgc
    15481 caccacgcca gccatccggt gcatgaacag gaacgccgcc agcacgacgc ccacctggat
    15541 cgcgacggtc aggtcgaaca caacggtcag cacgaaggtg agcagcagga ccgcgcgatc
    15601 gccgatcggc cccgacatca ggtgccggaa gctttcgatc tcggccatgt tccatgccac
    15661 caccacgagg atcgcggcca gcgtcgccag cggcacgtac tggatcagtg gcgccagcac
    15721 gagcatgaag accagcagga atgccgcgtg cagcattccg gcaatgggcg tccgcgcgcc
    15781 ggaccggaca ttggtggcgg tgcgcgcgat cgcgccggtg gccggcaatc ccccgaacaa
    15841 tgcgctgccg atgttggcaa cgccctgggc aaccagttcg caattcgagc ggtggcggcc
    15901 gccgatcatg ccgtcggcga ccaccgccga cagcaacgat tccacgccgg ccaggaacgc
    15961 gatcgtgaag gacaccggca gcaactcgcg ggtgcgctcg aacgggatgt gcgggaacga
    16021 gaaagtcggc aacgcgtccg gaatgccgcc gaaacgggtg ccgatcgtgt cgaccggcag
    16081 tccgatcagg gtgacgaccg cggcgccgcc caccacgacg atcaggaagc ccggccagtt
    16141 gggccgatac ttgcgcagca gcaggatcac cgccacgcat gccacggata ccgcaacggt
    16201 cgccgcgctg aagcccggca ggtgctgcgc ataggccgac cagcgcgcga agaactcggc
    16261 cggcaccctg gccatgtcca ggcccaggag gtccttcacc tggctcgaaa agatgctgac
    16321 cgcgatgcct gcggtgaagc ccgtgaccac cggtttcggg atgtacttca gcagcacccc
    16381 gaggcgcagc acgcccgccg cgatcagcat gcagccggcg agcagggtgc acaggatcag
    16441 cccgccgaag ccatgcttgt cgatgacgtt gaacaccacc gggatgaacg ccgccgtggg
    16501 cccgcccacc tggacgcgcg agccgcccag cagcgaaatc aggaagccgg cgacgatcgc
    16561 ggtgtacagg cctttctcgg gcgtcgtgcc ggaagcgatc gccagcgcca tcgccagcgg
    16621 cagggccacc acggccacgg tcaggccggc caccaggtcg tgccggaaat cgtcgaagtg
    16681 gtagccgcgg cgcaacagcg tcaccagctt ggggacgtac aactgcaggc tcgatgcggc
    16741 cgcaggctgc gacgtggaca tggctatccg cgcgaaggaa caaaagcgcg cactctaatc
    16801 cgaaccgatg gccttgtctc gcccggcatc acggatcctg cggctggtgc ccggccggtg
    16861 cgagcgctag ggaatgcgtt ttgcggcgcg gtcggtgccg ttctggtgca ggaccagggc
    16921 atcgaccttg ccgtccgcgc cgcgtttgaa gctgagccgg gcgtccacga ccgtgtagta
    16981 gaactcgtcc ttcgcgctgg ggtagaccgg ggccgccggc tgtccggtca gctgcgcctt
    17041 cagttggccg tggtcgagcg tgatgtcgaa taccgcgccc ggtgccagtt gataacggcc
    17101 gacgtactgc ttcagtgtcg cggtgtcgag gcggacggtc ttgtggccgc cgagctccgc
    17161 ggattcggcg gcatcgagtt tcggcgccgg catgtcgtgg ccgttctggt gcagcaccag
    17221 tgattccacc ttacccttgc cgtcgcgttt gaagtcgatc cggatgtcgg tgatctttgc
    17281 aaaaaacttg tcggtggcgt ccgggaagat cgggaatgcg ccctggccgg tggcctgcgc
    17341 gagcagctgg tcgccttgct gcatcaccgt caacacgaag tccggggcga gccggtactc
    17401 gccgatgtag tcctgaagct gtttcggggc caaggcgatt tgtttgcggg ccggcgccag
    17461 gggcgcgtcc gggagcagcg cggcgaagcc gaggtcgtcc accgattgct ggatgttggt
    17521 taggatcatc acgccgcgcg ttccgtcggc ggtgaagccc aggaagctcg cgtagccgcc
    17581 ggtcatgccg ttgtgccaga ccacatcgcc cgacttgccg cgtttcgtca tccacgcgag
    17641 tccgatgcgc tcgtcgccgc ccaccgcgcg gcgtggttcg tgcgcgaact tcatcgcgcg
    17701 ccgcagtgcg ccttcgccac ggcccatgtt ggcttcgagg tagcgcagca tgtccgtgcc
    17761 ggtgctgttg atcgcaccgg cgccggccag cgcgtcgaga tgccagggtt gcgcgggctt
    17821 gccttgctcg tcgtggcccg gcgcccacct cggagcgagc ggttccttca gggtggcgct
    17881 ggtcgacgtc atgccgagcg gtccgaagac ccgcgattgc aggagttgcg ggtaggaggt
    17941 cccggcgtgc cgggcgaggg cgtagccgag caggcccagc ccgagattgg aatattcgta
    18001 cttcgcgccg gggtcgcgcg ggagcttgta gccggcgagg aacgccttca gcttcgcggc
    18061 atcgtaatcc gcgtagggat cccgaggatc ggcaggc
//
LOCUS       DASSNH010000130         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_958434, whole genome shotgun sequence.
ACCESSION   DASSNH010000130 DASSNH010000000
VERSION     DASSNH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958434"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFK00_04795"
     CDS             <1..310
                     /locus_tag="VFK00_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HET7592305.1"
                     /translation="KGSRFVSHEAACLLNANDRDAHVAITLYFVEKEPVGPYKVRVPA
                     RRTLHLRFNDLEDPEPVPRDTPYSSVIESDVPIVVQHTRLDSRKEALGLLSTIAWSAI
                     "
     gene            400..768
                     /locus_tag="VFK00_04800"
     CDS             400..768
                     /locus_tag="VFK00_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592306.1"
                     /translation="MAEDTNKPQAHANGKTHERIDRAAERIKQEAGEAAQAAKAKTDH
                     AEQRAEHATDAGARTAHRLADKAADWRQRGSAAAYTMRDRADNLMEDARECVREKPVQ
                     SVAIALAAGWLVGRLLGSRR"
     gene            800..1336
                     /locus_tag="VFK00_04805"
     CDS             800..1336
                     /locus_tag="VFK00_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592307.1"
                     /translation="MSIADGREPGAAPGRPPPPPEGAPAGEGGAPSPSTLAALAGVFA
                     ALRQLFFALRKVATAEAKVVRAGIPLLFIAATALVAFAVSLWVCLVALIGWALMHATH
                     SPGLALGLLVVIHAILIVGVWSMIKYALRQATFPQARAELRMLGRTVLHDLGRIAGTR
                     EAVRGAPPEAAPGRKENP"
     gene            1333..1596
                     /locus_tag="VFK00_04810"
     CDS             1333..1596
                     /locus_tag="VFK00_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592308.1"
                     /translation="MNKYFHALRARRRALRECEAARRELHGSVEDLVAAYEARPWPIL
                     AGAAGIGFVLAQVRIGSGMVKAGARVAAGPAWGLARQFVDHIG"
     gene            1790..2206
                     /locus_tag="VFK00_04815"
     CDS             1790..2206
                     /locus_tag="VFK00_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592309.1"
                     /translation="MLGAIVATSAWATDANEIKCQMTFTLSGWSVIYKTADGTGTVTC
                     DNGQSMAVHLDAKGGGLTVGKYKLTEGHGEFSGITDINQIVGTYATASAHAGVVNSGH
                     VAAMTKGNVSLKLSGKGEGWDIGAALSGFTIHKADQ"
     gene            2374..2850
                     /locus_tag="VFK00_04820"
     CDS             2374..2850
                     /locus_tag="VFK00_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7592310.1"
                     /translation="MDAIKLLKEDHDKVKELLSSLAETTARAEKKRTELLSKITEELE
                     VHTRIENEIFYPAFKKAGEKSDDAKMFFEAREEHRAVGELVLPDLNKTPAGSEQFSGR
                     AKVLKELVEHHVKEEEGEMFPRARKLMSKDELQALGEQMETRKQELMKQRSPRAAA"
     gene            2996..>3373
                     /locus_tag="VFK00_04825"
     CDS             2996..>3373
                     /locus_tag="VFK00_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HET7592311.1"
                     /translation="MKSGSGAQELRYSSDFEPGISRLRAGRGFAYRDPSGRPVRDRAT
                     LQRIRALAIPPAYRDVWICTDPAGHLQATGRDARGRKQYRYHPQWRIQRDTEKYRRLL
                     RFGRALPALRRRLRSDLARKGLPR"
BASE COUNT          617 a         1105 c         1170 g          481 t
ORIGIN      
        1 gaagggctcg cgtttcgtca gccacgaagc ggcctgcctg ttgaacgcca acgatcggga
       61 cgcgcacgtg gcgatcaccc tgtatttcgt cgaaaaggag ccggtcggtc cctacaaggt
      121 ccgggttccg gcccgtcgca ccctgcactt gcgcttcaac gacctcgaag accccgagcc
      181 cgtgccgcgc gatactccgt attcgagcgt gatcgaatcg gacgtgccga tcgtggtcca
      241 gcacacccgg ctggattcgc gcaaggaagc gctcgggctg ctcagcacca tcgcctggtc
      301 agcgatctga agggggcttg tccggaccgc ggcacgcgcg caagccaccc gcaatcgaac
      361 gccgccgcct cgaggcggca ccaccggaag gagaagatca tggccgaaga caccaacaaa
      421 ccgcaggcac atgcaaacgg caagacccac gaacgcatcg accgcgcggc cgagcggatc
      481 aagcaggagg ccggcgaggc cgcgcaagcc gccaaggcca agacggacca cgccgagcag
      541 cgcgcggaac acgcgaccga cgcgggcgcc cgcaccgccc accggctcgc cgacaaggcc
      601 gcggactggc ggcagcgcgg cagcgccgcc gcgtacacca tgcgcgatcg cgcggacaac
      661 ctgatggaag atgcccgcga gtgcgtgcgc gagaagccgg tgcaatcggt ggcgatcgcg
      721 ctggcggccg gctggctggt cggccggctg ctgggttcgc gccgttgaag accgcccgca
      781 tgccgcggcg gacaggccag tgagcatcgc cgatggccgc gagcccggcg ccgcgccggg
      841 ccgtccgcca ccgccaccgg agggtgcgcc cgccggtgaa ggcggggcgc cgtcaccctc
      901 gacgcttgca gcgctggcgg gggtgttcgc ggccctgcgg cagctctttt tcgcgttgcg
      961 gaaagtcgcc accgccgagg ccaaggtggt gcgcgcgggc atcccgctgc ttttcatcgc
     1021 ggcgaccgcg ctggtcgcat tcgcggtgtc gctgtgggtg tgcctcgtgg ccttgatcgg
     1081 ctgggcgttg atgcatgcaa ctcattcgcc gggcctggcg ctgggcttgc tggtggtgat
     1141 ccatgccatc ctcatcgtgg gcgtctggtc gatgatcaag tacgcgctcc ggcaagcgac
     1201 gttcccccag gcgcgcgccg aattgcgcat gctcggccgg acggtcttgc acgacctcgg
     1261 ccgcatcgcc ggcacgcggg aggcggtgcg gggcgcgccg cccgaagctg cgccgggaag
     1321 gaaggaaaac ccatgaacaa gtacttccac gcgttgcgtg cgcggcgccg ggcgttgcgc
     1381 gaatgcgagg cggcgcgacg tgagctccac ggatcggtgg aagacctggt ggcggcgtac
     1441 gaggcgcgtc cgtggccgat cctggccggc gcggccggga tcggcttcgt gctcgcgcag
     1501 gtgcgcatcg gcagcggcat ggtgaaggcg ggcgcgcgcg tggcagccgg tccggcctgg
     1561 ggcttggcgc gtcagttcgt cgatcacatc ggctgatccg gccccccaca acgcacgccg
     1621 ttcggccacg gtgggtccgc agcgcgaggc ccgggtggcc acggcgtttc acaccgtcaa
     1681 cacgcgcccc tgcctaggat cacaggcgga cccgaagcgt ggtcatggaa ccagatcaag
     1741 gaggagtacc gtgaaagaca agcgattggg attcgcattg gcgctgtcga tgctgggcgc
     1801 gatcgtcgcg acgagcgcgt gggcgacgga cgccaacgaa atcaaatgcc agatgacgtt
     1861 cacgttgtcc ggctggtcgg tgatctacaa gacggccgac ggaaccggga cggtcacctg
     1921 cgacaacggg cagtcgatgg cggtccacct cgacgcgaaa ggcggtggcc tgaccgtcgg
     1981 caagtacaag ctcaccgagg gccacggcga gttcagcggc atcaccgaca tcaaccagat
     2041 cgtgggtacc tacgccaccg cgagcgcgca tgccggcgtc gtgaattccg gccacgtcgc
     2101 ggcgatgacc aagggcaatg tttcgctgaa gctgagcggc aagggtgaag gctgggacat
     2161 cggtgcggcg ctgtcgggct tcaccatcca caaggccgac caataatcga cgaacgcgac
     2221 gatttcacga gcaatcacgt tgcgcgaccg tacccccggt cgcgcttttt ttgaacggca
     2281 acaccacccg gcatgggcag gcggcgccgc attcattccg cattaaccgc gtgccccgcc
     2341 aactatggat tccacccacg gagagacatg caaatggacg cgatcaagct gttgaaggaa
     2401 gaccacgaca aggtgaagga actgctgtcg agcctcgccg aaacgacggc gcgtgcggaa
     2461 aagaaacgca ccgagctgct ttcgaagatc accgaggaac tcgaggtcca cacgcggatc
     2521 gagaacgaga tcttctatcc ggcgttcaag aaggccgggg aaaagagcga cgatgccaag
     2581 atgttcttcg aagcgcgtga ggaacaccgc gcggtcgggg aactcgtgct gcccgacttg
     2641 aacaagacgc ccgccggctc cgagcagttc agtggccgtg ccaaggtcct caaggaactc
     2701 gtggagcatc acgtcaagga ggaggagggc gagatgtttc cccgtgccag gaagttgatg
     2761 tcgaaggacg aactgcaggc gctcggcgag caaatggaaa cgcgcaagca ggagctcatg
     2821 aagcagagga gcccacgcgc cgctgcatga tcgacggacg gtgccgtcgg cttccggccg
     2881 ccggcgccgc catcccctca tttgcgtgga agctttcggc ggcatgccgc cgagaggatg
     2941 atgtggcttg ggcgcaccgc gcaaccgcag cggcaagcgg cgtacggcga accggataaa
     3001 gtccggctcc ggcgcgcagg agctgcgcta ctccagcgat ttcgagccgg gaatatcgcg
     3061 gctgcgggca ggacgcggct ttgcctatcg cgatccttcg ggccggccgg tccgcgaccg
     3121 cgcgaccttg cagcggatcc gggcgttggc gattccgcct gcctaccgcg acgtctggat
     3181 ttgcacggac cccgccggcc acttgcaggc gaccggacgc gacgcgcgcg gacgcaagca
     3241 ataccgctac cacccgcagt ggcgaatcca gcgggatacc gagaagtacc ggcggctgct
     3301 tcgttttggg cgcgcgctgc cggccttgcg ccggcgcctc cgcagcgacc tggcgcggaa
     3361 gggccttcca cgc
//
LOCUS       DASSNH010000131         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_256655, whole genome shotgun sequence.
ACCESSION   DASSNH010000131 DASSNH010000000
VERSION     DASSNH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256655"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFK00_04830"
     CDS             complement(<1..455)
                     /locus_tag="VFK00_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HET7592312.1"
                     /translation="MTIKIDKKIKAYQVLKQEPTEAEKAPPVVKPAAAEVIQMHESLE
                     RPQTLIGATYKIKSPLFEHALYVTINDIVLNAGTEYEQRRPFEIFINSKNMDHFQWIV
                     ALTRIMSAVFRKGGDVTFLVEEMKAVFDPRGGYFKAGGVYMPSIVAEIGA"
     gene            complement(485..2632)
                     /locus_tag="VFK00_04835"
     CDS             complement(485..2632)
                     /locus_tag="VFK00_04835"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HET7592313.1"
                     /translation="MSTVRKSALNHDAADIPLQPASHDIWDKKYRLKTKQGEPVDVTV
                     DDTWKRVARALADVETTPELKEAWYERFLWALRRGAIPAGRITSNAGAHKHKPKTSTI
                     NCTVSGTITDSMDDILEKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGPLS
                     FMDIYDKMCFTVSSAGGRRGAQMGTFDVRHPDVREFIRAKREDGRLRQFNLSLLITDG
                     FMQAVEADADWPLVFPVHVKEKHEVDLADESQVIWREWPTRENYVSREDGLVACKIYG
                     HVRARHLWDMIMTSTYDYAEPGFILIDRVNEMNNNWWCEHIRATNPCGEQPLPPYGSC
                     LLGSVNLTTFVRDPFGPKARFDWDEYREVVKVFTRMLDNVVEINGLPLEQQRSEIMNK
                     RRHGMGFLGLGSTLAMLKHRYGSTEGVEFTERVAREMAVAGWEVALELAEEKGAAPVL
                     TRDYEVTAEMLRKRPEMAADGWKVGDKIRGSVLHAKYSRYMQRVAEAAPELVERLAET
                     GARFTHHTSIAPTGTISLSLANNVSNGIEPSFAHHYSRNVIREGRKTKEKVEVYSFEL
                     LAYRAWINAGAMPFTEDAGKKLPDYFVAADDIKPTEHVDMQAAAQKWVDSSISKTANV
                     PTEYPYEDFKDIYLYAYKRGLKGCTTFRFNPEAFQGVLVKEADLENTRYQFELEDGSV
                     IEVKGNEEVEYDGETHTAANLFDALKEGYYGKY"
     gene            3140..>3503
                     /locus_tag="VFK00_04840"
     CDS             3140..>3503
                     /locus_tag="VFK00_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7592314.1"
                     /translation="MHADATQPPMVDGKPMMSLAPMLAHVTPAVVNISSTTHIRVRDP
                     YFNDPLFRRFFGGGVPRERIEQSLGSGVIVDAAKGYILTNNHVVSGADAIKVTLHDGR
                     HFKATLVGADPDTDIAVLK"
BASE COUNT          563 a         1093 c         1137 g          710 t
ORIGIN      
        1 gcgccgatct cggccacgat ggagggcatg tacacgccgc cggccttgaa gtagccgccg
       61 cgcgggtcga acacggcctt catttcctcg accaggaagg tgacgtcgcc gcccttgcga
      121 aacaccgcac tcatgatgcg ggtcagcgcc acgatccact ggaagtggtc catgttcttc
      181 gaattgatga agatctcgaa gggacggcgc tgctcgtatt cggtgccggc gttgagcacg
      241 atgtcgttga tggtgacgta aagcgcgtgt tcgaacagcg gcgacttgat cttgtaggtg
      301 gcgccgatca gggtctgcgg ccgctccagg ctttcgtgca tctggatgac ttcggcggcc
      361 gcgggcttga cgaccggcgg cgccttttcg gcttccgtcg gctcctgctt gagcacctga
      421 taggccttga ttttcttgtc gatcttgatg gtcatggcga tctctttgtg gtgtccaacg
      481 gcaatcagta cttgccgtag tagccttcct tcagcgcgtc gaacaggttg gcggcggtgt
      541 gggtttcgcc gtcgtattcg acctcctcgt tgcctttcac ctcgatcacg ctgccgtctt
      601 ccagctcgaa ctggtagcgc gtgttttcga ggtcggcctc cttgaccagc acgccctgga
      661 acgcctcggg attgaagcgg aaggtggtgc agcccttgag gccgcgcttg taggcgtaga
      721 ggtagatgtc cttgaagtcc tcgtagggat actcggtggg cacgttggcg gtcttggaaa
      781 tggacgaatc cacccatttc tgcgccgccg cctgcatgtc cacgtgctcg gtcggcttga
      841 tgtcgtcggc ggccacgaaa tagtcgggca gcttcttgcc ggcatcctcg gtgaagggca
      901 tggcgccggc gttgatccag gcgcgatagg ccagcagctc gaagctgtag acctcgacct
      961 tttccttggt cttgcgcccc tcgcggatga cgttgcgcga atagtgatgc gcgaagctcg
     1021 gctcgatgcc gttgctgacg ttgttggcca gcgacagcga gatggtgccg gtcggcgcga
     1081 tggaggtgtg gtgggtgaaa cgcgcgccgg tttcggcgag tctctcgacc agctccggcg
     1141 cggcctcggc gacgcgctgc atgtagcggc tgtacttggc gtgcagcacg gacccgcgaa
     1201 tcttgtcgcc gaccttccag ccgtcggcgg ccatttccgg acgcttgcgc agcatttcgg
     1261 cggtcacttc gtagtcgcgc gtcagcaccg gcgcggcgcc tttctcctcg gccagctcca
     1321 gcgccacttc ccagccggcc acggccattt cgcgcgccac ccgctcggtg aactcgacgc
     1381 cttcggtcga gccgtagcga tgcttgagca tggccagcgt cgaaccgaga ccaaggaagc
     1441 ccatgccgtg acggcgcttg ttcatgatct cgctgcgctg ctgttccagc ggcaggccgt
     1501 tgatctcgac cacgttgtcg agcatgcgcg tgaacacctt caccacctcg cgatactcgt
     1561 cccagtcgaa gcgcgccttg ggcccgaacg gatcgcgcac gaaggtggtc aggttgaccg
     1621 agccgagcag acaggaaccg tacggcggta acggttgctc accgcaaggg ttggtcgcgc
     1681 ggatgtgctc gcaccaccag ttgttgttca tttcgttgac gcggtcgatg aggatgaagc
     1741 cgggctcggc gtagtcgtac gtcgaggtca tgatcatgtc ccacagatgc cgcgcgcgga
     1801 cgtggccgta gatcttgcag gcgaccaggc cgtcctcgcg ggaaacgtag ttttcgcggg
     1861 tgggccattc gcgccagatc acctggcttt cgtcggcgag gtcgacctcg tgcttttcct
     1921 tgacgtgcac ggggaacacc agcggccagt cggcgtcggc ttccacggcc tgcatgaagc
     1981 cgtcggtgat caggaggctc aggttgaact ggcgcaggcg gccgtcctcg cgcttggcgc
     2041 ggatgaactc gcgcacgtcg ggatggcgca cgtcgaaggt gcccatctgc gcaccgcgac
     2101 ggccaccggc ggagctgacg gtgaaacaca tcttgtcgta gatgtccatg aacgacagcg
     2161 ggccggaggt gtaggcgccg gcgccggaca cgtaggcgcc gcgcgggcgc agcgtcgaaa
     2221 attcataccc gataccacac cccgccttga gcgtcaggcc cgcctcgtgc actttttcca
     2281 ggatgtcgtc catggaatcg gtgatggtgc ccgacacggt gcagttgatg gtgctggtct
     2341 tgggcttgtg cttgtgcgcg ccggcgttgg aggtgatgcg gccggcggga atggccccgc
     2401 ggcgcagcgc ccacaggaag cgttcgtacc aggcctcctt gagttcgggc gtggtttcga
     2461 cgtcggccag cgcgcgggcg acgcgcttcc aggtgtcgtc cacggtgaca tccaccggtt
     2521 cgccctgttt ggtcttgaga cggtatttct tgtcccagat gtcgtgcgag gcgggttgca
     2581 gcggaatgtc cgccgcgtcg tggttcaggg cgctcttgcg tacggtgctc atcggtatcc
     2641 ttttcgatct cggatgcgaa gggtctgact actgttgccg gtgccccgca gggcaaggct
     2701 catcaaaagt cggagtcggg gtcgccgccg tatcgaaacc gatgacgacg aacagtggat
     2761 gacaaagaca catggccacc tccggatacg ttgcccgccg cccctgtacg gcccgcactc
     2821 aaccccatga cttgtgaatc gcctgaaaac cggacacaag atgttgtgtc cggcagccgc
     2881 tccaaggtac ccccgcaggc gggcaaagtc aaagcgaaat attcgacccg cgcgatggcc
     2941 cgctgcacgg cccttcacgc ctgttgcagt atgctcgccg aggccgggca aggactgaac
     3001 cacgcatggc cgacgcctgt ccaaaccggt cggattcacg ttttttgcaa ggagtcgttc
     3061 catgcgcgca cgttcccgcc ggcgccgttc gccgcttctg ttgacatggc tcgtgatcgg
     3121 gctcgcgttg tggctgccga tgcatgccga cgccacgcag ccgccgatgg tcgacggcaa
     3181 gcccatgatg tcgctggcgc cgatgctcgc ccacgtcacg ccggccgtgg tgaacatctc
     3241 ctcgaccacg cacattcgcg tgcgcgatcc gtatttcaac gacccgctgt tccgccgctt
     3301 tttcggcggc ggggttccgc gcgaacgcat cgagcagagc ctcggctcgg gcgtgatcgt
     3361 ggatgccgcc aagggctaca tcctgaccaa caaccacgtg gtcagcggcg ccgatgcgat
     3421 caaggtgacg ctgcacgacg gacgccactt caaggccacc ctggtcggcg ccgatccgga
     3481 caccgacatc gcggtgctga aaa
//
LOCUS       DASSNH010000132        15712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1527078, whole genome shotgun sequence.
ACCESSION   DASSNH010000132 DASSNH010000000
VERSION     DASSNH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527078"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..144
                     /locus_tag="VFK00_04845"
                     /pseudo
     CDS             1..144
                     /locus_tag="VFK00_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
     gene            727..1806
                     /locus_tag="VFK00_04850"
     CDS             727..1806
                     /locus_tag="VFK00_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7592315.1"
                     /translation="MTTSCSTRLDWEPFGPAAGVRCMLLAAGMALMLAGCGQGSNEAS
                     SAPPATNAEPTTSAAPVSAPVSSAPAALPTAAPAVATTAARAQQLASATQARFQRMSV
                     ADLLAAARQAYAQHRLVTPAGNNAMEYYEAVLAKDPANQVARDALRETFPFGVPDVEK
                     AISQNDFDEAAREIDLLSRADPTNYTLTLLRSKLDAQRKLQARQEQAQLQAQQAAQLA
                     AAQQAAAAKAAAEKEAAARKAAEQQAAEVAKAAPPPKPTAPAPTPAKPAGESRNAVAV
                     SPAAPEYPLQAARNQTSGYAVVEFTVSIDGTVDNPHVIDSSPRRVFDSAAIQAVRSSK
                     FKPALKDGEPVASTLRRRIDFKFGG"
     gene            complement(2062..3312)
                     /locus_tag="VFK00_04855"
     CDS             complement(2062..3312)
                     /locus_tag="VFK00_04855"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7592316.1"
                     /translation="MSAFCAGLACTLVSGLALAAPGVQSNPVPSQPTVVHSTRQAHLP
                     DARVDAALKEYGHWLDQLAADGRTAGLATAVVVDGKVRYERTLGFADANEQAPVTPDT
                     VFRLASLSKAFATAVTGMLVRRGVLSWDTRLSSVLPFFKLRDANASQEATVRDILSQS
                     IGLPPNAYDNLLSSGVPYQELERKLATVKMTCSPGDCYGYQNIAFSLIGDVIHAKTGQ
                     FFIQLVDRYLFLPLGMTTASYGRDGLEGSRSWARPHYKRNGKWIAYEPNTHFYVPPAA
                     GVNASIRDMEQWLIAQMGGRPMVLPEALLEVLHAPIIDTPSERMFSNWRRARVKKASY
                     ALGWRVYDYEGHTLIFHAGAVQGYRSMIGFFPEYHTGVVVLWNCENGSPSGLMPMLLD
                     AFLGLKHEDWAGLDRRPRSSRRKR"
     gene            3365..3952
                     /locus_tag="VFK00_04860"
     CDS             3365..3952
                     /locus_tag="VFK00_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7592317.1"
                     /translation="MGWIIVLIIIALIVIYLIAIYNGLVTSRNAYKNAFAQIDVQLTR
                     RHDLIPNLVETAKGYITHERETLEAVTQARTAAVNGLAAAKAQPGDPNAMKQLSGSEN
                     QLTQALGRLFAVSEAYPDLKANQNMMQLSEELTSTENRVAFARQAYNDSVMNFNNKVQ
                     VFPNTLVAGPFGFTPAEPLAIDDPAAREAPKVSFA"
     gene            4179..6176
                     /locus_tag="VFK00_04865"
     CDS             4179..6176
                     /locus_tag="VFK00_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7592318.1"
                     /translation="MDFFAQQAKVRRSSRTLVLLFAVAVLAIVVAVDLICAFVLGVGL
                     RPGPLLGIAAAVVVVIGLCSLYRVTSLAGGGAAVARSMNATEVPADTTDPQWRRLRNV
                     IEEVAIASGVPVPDIYVMQDEPAINAFAAGYTPNDAAVCVTQGCLDKLDRDEMQGVIA
                     HEFSHVLNGDMRLNVRLIGLLFGIMAIGVIGRYVLWGGYLGGGRRRRDGGGNGNVVLA
                     GMALLVVGAIGLFFGRLIQAAVARSRESLADASAVQFTRQTRGIAGALKKIAAITEGS
                     ALQTPRRDEVRHMLFGDAEQRSWFATHPPILERIRALDPSFDPKELDAINEKWLAAGG
                     DPDSISLQPADTGWRRSAHDLAAADGDADVSGPLPGFPPAPAGPAGGPLPMQGAVGAI
                     TAAGVLAQVGSPGRDDYQAATQLHARIPPAVAAAARDPRGALAVLLALAISEHPDLKS
                     QQLQIVSKSFDAASAEATQRLVAALADLHPMLRLPLAQVAFPALKRRPRPEIDRFIKT
                     LDALIHADGRVDLDEFCLAKLVQSQVVAALDPSAHFKPGTRKLPDVRDELRDLAALVA
                     HFGNDDEDGARQAFQAAMQEALPGAPVMYALPDDWQAALDRALDALARLAPEGKQLTV
                     RALTRAIAADGKVTLAESELLRVVCAELGCPLPPVLAGAAA"
     gene            complement(6263..6859)
                     /locus_tag="VFK00_04870"
     CDS             complement(6263..6859)
                     /locus_tag="VFK00_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HET7592319.1"
                     /translation="MNHLAHALLADAGGAEFALGSAMGDFVHGRPDTTWPAGRQAGLR
                     FHRAIDGFTDRHPDVVAARRLFDPPFRRYAGILLDVWFDHLLVLGWNRFVVAGPLDAF
                     AGRWLALLDARAADLPASLRGFIRWMRAHDLPAGYGDEATLAEVFRALARRLSRPSPI
                     GDALPVLRAQSDALQPLFDAFFPDLMAHARDLRRGLLE"
     gene            complement(6940..7302)
                     /locus_tag="VFK00_04875"
     CDS             complement(6940..7302)
                     /locus_tag="VFK00_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HET7592320.1"
                     /translation="MAYVDGFVIAVPNANKAAFIRHARKADPVFVEYGARRVLECWGD
                     DVPEGKVTDFRRAVQAAQGETVVFSWIEWPDKATRDAGMAKVMQDPRLAPDDCKSIFD
                     GQRMIFGGFEPVVDIAGA"
     gene            7474..8154
                     /locus_tag="VFK00_04880"
     CDS             7474..8154
                     /locus_tag="VFK00_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET7592321.1"
                     /translation="MSEPLPPAPAGFPDSSTTFALPGPAGPLEVAVAVPESDCARAGT
                     AVICHPNPQQGGTMRNKVVTMLERSLRESGLATVRFNFRGVGASAGAFDNGDGESGDL
                     VAVAQWVRKNRPGDALWLAGFSFGSYVALRSARSLAADALILVAPPASMYDFDEIAAP
                     GCPWLVVQGEEDEVVDPQAVFDWIEKLDPKPTLVRMPDTGHFFHRRLMDLRGAVKNAV
                     RAWLPPPR"
     gene            8178..9311
                     /gene="zapE"
                     /locus_tag="VFK00_04885"
     CDS             8178..9311
                     /gene="zapE"
                     /locus_tag="VFK00_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HET7592322.1"
                     /translation="MDPPLNAPLDSPSTRYARGVAKGDWEDDPAQHRALAALDRLHAE
                     LLAPQPTGLLARLRARRARPTVRGLYLWGPVGRGKTLLMDQFAASLPEDAALRVHFHR
                     FMLDVHARLRALGEISDPLPRVAEEFAARSRVLCLDEFMVTDIGDAMILHGLLRALFE
                     QGVVLVTTSNTPPSALYRGGLQRERFLPAIALIERQCEVLELTSPHDWRLRVLTRAPV
                     YLTPDNAHAEAALGRLFGQLAQGPIQEGGELRIDDRAIQVRKAAANVAWFDFAALCDG
                     PYGSADYIELARIYPTILVSRLPQFTPFNEDAAQRFVHLVDELYDRRVKLAVTAQVPV
                     VELYDGRRLRAEFARTESRLIEMQSEAWLAESIDRGNIDCDRP"
     gene            9666..11156
                     /locus_tag="VFK00_04890"
     CDS             9666..11156
                     /locus_tag="VFK00_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7592323.1"
                     /translation="MSRTKPAAGWAAAALIAFALSGCAVGPNFREPAAPAVERYTPAA
                     LPASTASAATPGGDAQRFLEGREVPRRWWTTFGNEELDRRVETALAHSPTIASAQAAL
                     RQAEENARAAGGSLFPSLDASAGATRANQFTGAGAGAGGAVGSKPFTVYNAGVSVGYL
                     FDIFGGVRRGVEAQQALADVQRAQLDATYLTLAANVVTASLQEASLREQVRATRQIID
                     SLQQELDIAEKQQAIGTTSLTDTLAVRTQLATATVALPGLRKQLAATRNQLATYLGVT
                     PAQIDLQPITLDEVTLPADVPVSLPSTVVAQRPDIRAAAAQLHAATAGVGVATANMLP
                     QLTLSGSAGSQALHAGDLFTAGTSAWSIGLNILQPLFHGGELLHKKRAAEAGMDQALA
                     NWQQVVLAAFQDVADSLQALEYDAHSLRALATAEQDASRLLALTRSQYRVGAVGYLNL
                     LNAEQTWQQARISLIQARAARLADTAALYAALGGGWQQGEPVATKK"
     gene            11278..12501
                     /locus_tag="VFK00_04895"
     CDS             11278..12501
                     /locus_tag="VFK00_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7592324.1"
                     /translation="MNANQPSARPKLRKRLIIVISAVVVLFALLVGLHAFIGMKIGEA
                     MASMVNPPQTVSTMVARYQDWQPHVEAFGNVRAVHGADLAFDVSGLVDSVEVRSGADV
                     KKGDVLIKLNADQDRATLDALRARASLAEVTAKRARQQLAVKAISQASYDAAVADLKN
                     AKAQADAQAALVAKKTLRAPFAGRIGIITANPGQYINAGATVVTLQQLDPLYVDFTVP
                     QSSLRQMQVGGEVQVTADAFGDKAFKGEISATDPKVDVSTRNVGVEATVANPDELLVP
                     GMFAKVAVDAGREQRYLTLPQTSVAYAPYGDTVFVVHEGQPPARDANGKPVPAASAGN
                     KPEQDKDGTSHYVQQVIVQTGPTRGDQVAILSGIKEGDVVVTSGQLKLKNGTPVNVNN
                     KVEPAFDPNPHPVEE"
     gene            12612..15692
                     /locus_tag="VFK00_04900"
     CDS             12612..15692
                     /locus_tag="VFK00_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7592325.1"
                     /translation="MKFTDLFIKRPVLAATVSLLILVLGLRAVTSLPIRQYPKTESAT
                     ITVQTVYFGADAQTMAGFITQPLEQAISQAQGIDYLSSSSTPGVSMISARLRLNYDGN
                     KALTEISALVNSVKNQLPQAAQQPVITLQTGQQTASMYLGFYSSTVASNKITDYLTRV
                     VQPQLNAVDGVQSAQILGGRTFAMRAWLDPVKMAAHGVTATDVNAALASNNFLSAIGN
                     TKGQMVSVALTAATDLHTVDEFKDLVIKADGTDIVRLKDVARVTLGAENYDFNVAFDG
                     KRSVFIGINAAPDANVLDVISGVRKVFPTIQSQMPAGMTARIVFDGTDYIRTSIHEVV
                     QTLVETLLIVAVVIFLFLGTLRSLLIPLVAMPLSLIGAFLVMLVLGYSINLLTLLALV
                     LAIGLVVDDAIIVVENVDRHMKEGQPPLQAAILAARELAGPILAMAAVLVAVYIPVGF
                     QGGLTGALFTEFAFTLAGAVVISAIIALTLSPMMCSRSLRASDHEKPFVKRIDRVADA
                     VRDRYRRTLHSSLDTWLVAVVFGALILLGNVYLFKTSQSELAPQEDQGIVLTNTQGPP
                     NATIDQMALYSKQVFDIAKSLPEYTHIFQVDGAQGVNSGFAGIMLKPWGDRDRSATQI
                     QQAVQQKLGNIAGARVAVFQFPSLPGAFGLPVQFVIKTTEPFQNLNEVAKQVVAKAQA
                     SGMFFYIDSDLKIDQPQATVTLDRNKVSALGLTQRDVGAALSAALGGNYVNYFSISGR
                     AYRVISQVAQVDRMNPDQLKNYYIKTPEGGMIPASTVAHLTFQTVPESINHFQQLNSA
                     TIQGVIGPGVSLGQALQFMQDTLQEVAPSGYAADYAGQSRQFEQESGGFLVTMLFAII
                     IVFLALSAQFESFRDPIVILVSVPMATFGALLFINLGLATLNIYTEVGLVTLIGLISK
                     HGILMVQFANDAQRLHGMTKREAIEEAAAVRLRPILMTTCAMVLGVIPLVVAAGAGAA
                     GRHSIGLVIFTGISIGTLFTLFVVPAMYLLLGQDHHAEARVPERDVLPPPLPAE"
BASE COUNT         2636 a         5316 c         5264 g         2496 t
ORIGIN      
        1 catgtactcc ggctgctttc cgacgagcgt acacgtcgtc aagtgtccaa cctgcggcac
       61 gccacgaaca gcgatctttt gtgcgaggcg tttctgcatg cgttcactgg gcaaccactg
      121 ccaactgatg aggagctgag acgaacgatg gctaccaaga aagctgcaaa gagaaaaccc
      181 gccaagaagg cggccaagaa gaccacggcc cgcaagtccg cgaaggccaa aacctccacc
      241 gcgcgcaagt ccgcgcgcaa gaccgtccgc aagacggcca agaagacgac ggccaagaag
      301 ccggcacgca agcgcgccac caagaagacg gcacgcaagc gcaccgcgaa gaaggccgtg
      361 atggccaaga ctgcccgcgc cagcaagacc cggcgtgcga aggcgaagaa gaagtagttc
      421 ggcggcaccg ttgcacggtt ccacgcgaca tccataacga caagcctgct ctccccgcga
      481 cgcccagcgc ggggagccgc gggcaaacgg ttcatgtgtt ggccccggct ctgccggggc
      541 tttccatttt cgggatcgga caagtcgggc tcacaactgc ccgcccgacg cgcgaagagt
      601 cgaaccggtc acatcccggc catcgccggc tgtgagatcc ttcggccgcg gcggttctgc
      661 gcggtccatg gaacgaatcc tgcatggcca ccggggtcga attcctgact tcgtacaggt
      721 gcgcccatga ctacttcttg ctccactcgt ttggactggg aaccgttcgg gcccgcggcc
      781 ggcgtgcgtt gcatgttgct cgcggcgggc atggcactaa tgctggccgg ctgtggccag
      841 ggatcgaacg aggcatcctc cgcgccgccg gcgacgaatg ccgagcccac gacgtctgca
      901 gctcccgtct cggcaccggt gtcgtccgca cccgcggcgc tcccgaccgc cgcgcccgcc
      961 gtcgctacga ctgccgctcg cgcgcagcaa ctggcgagcg ccacgcaggc caggttccag
     1021 cgcatgagcg tcgcggacct gctcgccgcc gcgcgccagg cctacgccca gcatcggctg
     1081 gtgacgccgg cgggcaacaa cgcgatggag tactacgagg ccgtactcgc gaaggatcct
     1141 gcgaaccagg ttgcaaggga cgcgctccgt gaaacgttcc cgttcggcgt gcccgatgtc
     1201 gagaaggcga tttcgcagaa cgacttcgac gaagccgcgc gcgagatcga tctgttgtcc
     1261 agggccgatc ccaccaatta caccttgacg ctgctgcgct ccaagctgga tgcgcagcgg
     1321 aagctgcagg cgcggcagga gcaggcgcag ttgcaggcgc aacaggcggc gcaactcgcg
     1381 gctgcccagc aggccgcggc cgccaaggcc gcagccgaga aggaggccgc cgccaggaaa
     1441 gccgccgagc aacaggccgc cgaggtcgca aaagccgcac cgccgccgaa gccaaccgcg
     1501 ccggcgccga cgccggccaa gccggcgggc gaatcgcgga atgcggtggc ggtgtcgccc
     1561 gcggcacccg agtatccact ccaggcagca cgcaaccaaa cctcgggtta cgcggtggtc
     1621 gagttcacgg tgtccatcga cggcaccgtc gacaatccgc acgtcatcga ttcatcgccc
     1681 cggcgggtgt tcgattccgc ggcgatccag gcggtgcgga gctccaaatt caaacccgca
     1741 ttgaaggacg gcgagccggt cgcgagcacc ttgcgtcgtc gcatcgattt caagtttggt
     1801 gggtgagaag ccgcgctagg cgcggcttcg aacgaccggc catggatcgc cgggcgaaaa
     1861 cgcaccatgg atggttgctc gcctcatctc cggccggtcg aataccgcac catggatggt
     1921 tactcgccta ttcacgccgg gcgcatatcg cagcaggatg gttgcccgat ggctgtccag
     1981 gcgtgcgtgc gctcgacgtc gcgcgcattc caggcgcgac cgcctgacct gattgcgctt
     2041 cgctccctcc gggctacagg gtcaacgctt gcggcgcgag cttcgggggc ggcgatcgag
     2101 gccggcccag tcctcgtgct tcaggccaag gaaggcgtca agcagcatcg gcatcaaccc
     2161 gctggggctg ccgttctcgc aattccacag caccacgacg ccggtgtggt attcggggaa
     2221 gaatccgatc atcgagcggt agccctgtac ggccccggca tggaagatca gggtgtgccc
     2281 ttcgtagtcg tagactcgcc agccgagggc atacgaggcc ttcttgacgc gcgcacgtcg
     2341 ccagttcgag aacatccgct cggacggggt gtcgatgatc ggcgcgtgca gcacttccag
     2401 caacgcctcg ggcagcacca tcggtcgccc tcccatctgc gcgatcagcc actgctccat
     2461 gtcgcggatg ctggcattga cacccgcagc gggcggcacg tagaagtgcg tgttgggctc
     2521 gtaggcgatc catttgccgt tgcgcttgta gtgcgggcgc gcccaactgc ggctgccttc
     2581 cagtccgtcg cgtccgtaac tcgcggtcgt catgccgagc ggcaggaaca ggtagcgatc
     2641 gaccaactgg atgaagaact ggccggtctt ggcgtggatc acgtcgccga tcaacgagaa
     2701 cgcgatgttc tggtagccgt agcaatcacc cggcgagcag gtcatcttca ccgtcgccag
     2761 cttgcgttcg agttcctggt agggcacgcc gctcgagagc aaattgtcgt aggcgttggg
     2821 cggcaggccg atgctctggc tcaggatgtc gcgcaccgtg gcttcctggg acgcgttggc
     2881 gtcgcgcagc ttgaagaacg gcagcacgga tgaaaggcgc gtgtcccaac tgagcacccc
     2941 gcggcgcacc agcatgcccg tcactgcggt ggcgaacgct ttcgacagcg acgccagccg
     3001 gaacacggtg tccggcgtga cgggggcctg ctcgttggcg tcggcgaaac ccaaggtgcg
     3061 ctcgtagcgc accttgccgt cgacgacgac cgcggtggcg agccccgcgg tgcgtccgtc
     3121 ggccgcaagc tggtcgagcc agtggccgta ttccttgagc gccgcgtcga cgcgcgcatc
     3181 gggaaggtgc gcctgtcgtg tgctgtgcac gacggtcggc tgggatggaa ccgggttgct
     3241 ctggacgccc ggcgcggcca gcgccagtcc tgaaaccagc gtgcaggcca gccctgcaca
     3301 gaaagcggac aagtgcatgc tatgctcaaa ttaccgcgaa gcacgaaggg agtacacgac
     3361 aatgattggt tggatcatcg tcctgatcat catcgccctc attgtgattt acctgatcgc
     3421 gatctacaac gggctggtca cttcgcgcaa cgcatacaag aatgccttcg cgcagatcga
     3481 cgtccagctc acgcggcgcc acgacctgat cccgaacctc gtcgagaccg ccaaaggcta
     3541 catcacgcac gagcgcgaaa cgctcgaggc ggttacgcag gcgcgcaccg ccgcggtgaa
     3601 cggcctcgcc gccgccaagg cgcaaccggg cgatccgaac gcgatgaagc agttgtccgg
     3661 cagcgaaaac cagttgaccc aggcgctcgg ccgcctgttc gccgtcagcg aagcgtatcc
     3721 ggacctcaag gccaaccaga acatgatgca gttgtccgag gagctgacct ccacggagaa
     3781 ccgtgtcgcg ttcgcgcgcc aggcgtacaa cgattcggtc atgaacttca acaacaaggt
     3841 gcaggtgttc ccgaacacgc tggtcgccgg cccgttcgga ttcacgcccg ccgagccgct
     3901 cgcgatcgac gatcccgcgg cacgcgaggc gccgaaggtg agttttgcct gacggcggga
     3961 agcgggaagc agggagcggg atcagggaac ctggaagaac aacccagtcc ggcggtccct
     4021 cggattgccc ggagtccgca ggctgccgga tgtagcgctg gcccgaagac aaatctgtcc
     4081 tgattcccgg tttcgccttc cggttcccac tcgtcgttcc ctgttccccg ttcccgcttt
     4141 tcggtttcgc ctccctgttc ccgctcttcg gttcacccat ggactttttc gcccaacagg
     4201 ccaaggttcg ccgcagcagc cgcacgctgg tgctgctgtt cgcggtggcg gtgctggcga
     4261 tcgtggtcgc ggtcgacctg atctgcgcgt tcgtgctggg ggttggcttg cggcccgggc
     4321 cgctgctcgg catcgccgcc gcggtggtgg tggtgatcgg gctgtgctca ctctatcgcg
     4381 tgaccagcct ggccggcggc ggcgcggcgg tcgcgcgcag catgaacgcg accgaggtgc
     4441 cggccgacac caccgatccg caatggcgtc gcctgcgcaa cgtgatcgag gaagtcgcga
     4501 tcgcgtcggg tgttccggtt ccagacatct acgtcatgca ggatgagccg gccatcaatg
     4561 cgttcgccgc cggctacacg cccaacgatg cggcggtgtg cgtgacgcaa ggctgcctcg
     4621 acaagctcga ccgcgacgaa atgcagggcg tgatcgctca cgagttcagc catgtgttga
     4681 acggcgacat gcggctcaac gtgcgcctga tcgggctgtt gttcgggatc atggcgatcg
     4741 gcgtgatcgg gcgctacgtg ttgtggggcg ggtatctcgg cggcgggcgt cggcggcgcg
     4801 acggcggcgg caacggcaac gtggtcctgg ccgggatggc gctcctggtc gtcggtgcga
     4861 tagggctgtt cttcggaagg ttgatccagg ccgcggtggc gcgttcgcgc gaatcgctgg
     4921 ccgacgcttc ggcggtgcag ttcacccgcc agacccgcgg catcgcgggc gcactgaaga
     4981 agatcgcggc gatcaccgag ggttcggccc tgcagacgcc gcggcgcgac gaggtccggc
     5041 acatgctgtt cggcgacgcc gagcagcgct cgtggttcgc gacccatccg ccgatcctgg
     5101 aacgcatccg cgcgctcgac cccagcttcg accccaagga actggacgcg atcaacgaga
     5161 aatggctggc ggccggtggc gatcccgatt cgatttcgct gcagcctgcg gacaccggat
     5221 ggcgacggtc ggcacacgac ctcgcggccg ccgacggcga tgccgatgtt tcgggaccgc
     5281 tgcccggttt cccgccggct cccgccggcc ccgccggtgg cccgcttccg atgcagggcg
     5341 ccgtgggggc catcaccgcc gcgggcgtgc tcgcgcaggt gggctccccc ggccgggacg
     5401 actaccaggc cgcgacgcag ttgcacgcgc gcatcccgcc cgcggtggcc gcggcggcgc
     5461 gcgacccgcg cggcgcgctc gcggtgctgc tggcgctggc gatctccgag catcccgacc
     5521 tcaagtcgca acagttgcag atcgtctcga agtcgttcga cgctgcaagc gccgaggcga
     5581 cccagcgcct cgtggcggcc ttggccgacc tgcacccgat gctgcgcctg ccgctggcac
     5641 aggtggcttt cccggcgctg aagcgcaggc cgcgtcccga gatcgaccgc ttcatcaaga
     5701 cgctggatgc actgatccac gcagacggtc gggtcgatct ggacgagttc tgcctcgcca
     5761 agctggtgca gtcgcaggtc gtcgccgcgc tggatccctc cgcgcacttc aagccgggaa
     5821 cccgcaagct ccccgacgtg cgcgacgaat tgcgcgatct cgcggcgctg gtcgcgcact
     5881 tcggcaacga cgacgaggat ggcgcgcgac aagcgttcca ggcggcgatg caggaggcgc
     5941 tgccgggcgc cccggtgatg tacgcgctgc ccgacgactg gcaggccgca ctggatcgcg
     6001 cactcgatgc gctggcgcgc ctcgcgcccg aaggcaagca actcaccgtg cgcgcgctca
     6061 cacgcgcgat cgccgcggac ggcaaggtca cgctcgccga atcggaattg ttgcgggtgg
     6121 tgtgcgcgga gctgggttgc ccgttgccgc cggtgcttgc tggcgccgcc gcgtgatcgc
     6181 tgcagccagg gcgggcattg cccgccgatc ctcgctggcg catccgtcgc tggcgggacg
     6241 atgtccgccc ccaacgcaga cctcactcga gcaggccgcg gcgcaaatcg cgtgcgtggg
     6301 ccatcaggtc cgggaagaac gcatcgaaaa ggggttgcag cgcatccgac tgcgcgcgca
     6361 gcacgggcag cgcatcgccg atcggcgacg gacgcgaaag ccgccgcgcc agtgcccgga
     6421 acacttcggc gagcgtcgct tcatcgccgt agccggccgg caagtcgtgg gcgcgcatcc
     6481 agcggatgaa tccgcgcagc gacgccggca ggtcggcggc gcgagcgtcc agcagcgcga
     6541 gccagcgccc tgcaaacgcg tccagtggcc cggccacgac gaatcggttc cagccgagta
     6601 ccaggaggtg gtcgaaccac acgtcgagca ggatgcccgc atagcggcga aatggcggat
     6661 cgaacagccg gcgcgcggcc accacgtccg gatgccggtc ggtgaagccg tcgatcgcac
     6721 ggtggaaccg caatccggcc tggcgtcccg ccggccacgt ggtgtcgggc cggccgtgga
     6781 cgaagtcccc catcgcgctg cccagcgcga actcggcgcc gccggcatcg gcgagcaggg
     6841 cgtgcgcgag atggttcatg ccgcgatttt acggcgtgga cgcggcaagc gggccgatca
     6901 tgccattgtc ccgcggccgg atcgccttgg cgcggctgct tacgccccgg ctatgtcgac
     6961 cacgggctcg aacccgccga agatcatccg ttggccgtcg aagatcgact tgcagtcgtc
     7021 cggtgcgagg cggggatcct gcatgacctt cgccatgccc gcgtcgcgcg tcgccttgtc
     7081 gggccattcg atccacgaga acaccacggt ttcaccctgc gcggcctgca cggcacggcg
     7141 aaaatcggtc accttgcctt ccgggacgtc gtcgccccag cattcgagca cccggcgggc
     7201 gccgtattcg acgaataccg ggtccgcctt ccgcgcgtgc cggatgaacg cagccttgtt
     7261 ggcgttgggg acggcgatta cgaaaccatc tacataggcc atggattcct ccgcatcgtc
     7321 tggaaaacgg gtgcgccaga agggcgcctc gcaactacga cgatcgggcg ttcccgtaat
     7381 cgacaagatg ggcgcggccg ccgtggggcg gccaacctgg gccgccggcg gggccgcacg
     7441 cgcacgcgat gcgactcctt atatcatgcg cggatgagcg aacccctgcc gccggcgcca
     7501 gccggctttc ccgacagctc gacgactttc gcgttgccgg gtcctgccgg cccgctcgag
     7561 gtcgccgtcg cggtgcccga aagcgactgc gcgcgtgccg gcaccgcggt gatctgccac
     7621 ccgaatccgc agcagggcgg cacaatgcgc aacaaggtcg tgacgatgct cgagcgcagc
     7681 ctgcgcgaat cgggactcgc cacggtgcgc ttcaacttcc gcggcgtcgg cgcgtccgcc
     7741 ggcgcgttcg acaacggcga cggcgagagc ggcgacctgg tcgcggtcgc gcagtgggta
     7801 cgcaagaacc gcccgggcga cgcgctgtgg ctggcggggt tttcattcgg gagctacgtg
     7861 gccctgcggt ccgcccggtc gctcgccgcg gatgcgctga tcctggtcgc gccgcccgcc
     7921 agcatgtacg actttgatga aatcgccgcg cccggctgtc cctggctggt ggtgcagggt
     7981 gaggaagacg aggtggtcga tccgcaggcg gtattcgact ggatcgagaa gctggatccg
     8041 aagccgacgc tggtgcgcat gccggacacc gggcacttct tccatcggcg cctgatggac
     8101 ctgcgcggcg cggtcaagaa tgccgtgcgc gcgtggttac cgccgccgcg gtgaaggggt
     8161 cgggacctgc cgcaaccgtg gatccacccc tgaacgctcc cctcgattcg ccgagcaccc
     8221 gttacgcgcg gggagtcgcc aaaggcgact gggaagacga tccggcgcag catcgggcgc
     8281 tcgcggccct cgatcggctg cacgcggagc tgctggcgcc gcagccgacg ggcttgttgg
     8341 cgcggctgcg ggcgcgccgt gcccggccga ccgttcgcgg gctgtatctc tggggccccg
     8401 tcggacgcgg caagacgctg ctgatggacc agtttgcggc atcgctgccg gaagacgcgg
     8461 cgctgcgcgt gcacttccat cgcttcatgc tggatgtcca cgcgcggttg cgcgcgctcg
     8521 gcgaaatcag cgatccgttg ccgcgggtgg cggaggagtt cgcggcgcgt tcgcgggtgt
     8581 tgtgcctcga cgagttcatg gtcaccgaca tcggcgacgc gatgatcctg cacggcctgc
     8641 tgcgtgcgct gttcgagcaa ggcgtcgtgc tggtcacgac ctccaacacg ccaccgtcgg
     8701 cgctgtatcg cggagggctc cagcgcgagc ggttccttcc cgcgatcgcg ttgatcgaac
     8761 ggcagtgcga ggtgctggag ctcacctcgc cgcacgactg gcgactgcgt gtgctgacgc
     8821 gggcgccggt gtacctcacg ccggacaacg cgcacgcgga ggcggcgctg gggcgattgt
     8881 tcgggcaact cgcgcaaggg ccgatccagg aaggtggcga actgcgcatc gacgaccgcg
     8941 ccatccaggt gcgcaaggct gcggcgaacg tggcgtggtt cgacttcgcg gcgttgtgcg
     9001 acggaccgta cggctcggcc gattacatcg agttggcgcg gatctacccg acgatcctgg
     9061 tgtcgcgatt gccgcagttc acgccgttca acgaggatgc cgcgcaacgc ttcgtgcacc
     9121 tcgtcgatga actctacgac cggcgcgtca agctcgcggt gaccgcccag gtacccgtgg
     9181 tggagctgta cgatggccgc cggttgcgcg ccgaattcgc gcgcaccgaa agccggttga
     9241 tcgagatgca gagcgaggcc tggctggccg agagcatcga tcgcgggaat attgattgcg
     9301 atcgtccctg accgccgcgc cgctggacgc agggtcacgc cggaagcctt cggtgccagc
     9361 caacgccgga acggccgtcc atgtcctgat tttccacaac agcggctccg gacgagacat
     9421 ttgtctcatg cgtcgaacgc cttcccggac tcgatgtcga attgcggcgc cgtcgctcgt
     9481 cgtcgaggca tcctcctgac aggtgtcacc cgccggaacg tgacatccgg cgcttacatc
     9541 cgccgcctgc gcggttgcag aatacgcatg caacaaatac aatactcgcc ggttcattta
     9601 tagaaccact ccgcgctgct ttcagaacgc cccgaatcgg attcccggcc tgaatgactc
     9661 cccccttgtc cagaaccaag cccgccgcag ggtgggccgc ggcggcgctg atcgcgttcg
     9721 cattgtccgg ctgcgccgtg ggtcccaatt ttcgcgagcc ggccgcgcct gcggtcgagc
     9781 gctacacgcc ggcggcgctg ccggcttcca cggcgtcggc cgcgacgccg ggcggcgatg
     9841 cgcagcgttt cctcgaaggc cgcgaggttc cgcggcgctg gtggaccacg ttcggcaatg
     9901 aggaactcga ccgccgggtc gagaccgcgc tcgcgcacag cccgacgatc gcatcggccc
     9961 aggccgcgct gcgacaggcc gaagagaatg ccagggcggc gggtggcagc ctgttcccct
    10021 cgctcgacgc cagcgccggc gcgacccgcg ccaaccagtt caccggggcg ggtgccggag
    10081 ccggcggtgc cgtcggatca aaacctttca ccgtttacaa cgccggcgtc agcgtcggct
    10141 atctgttcga catcttcggt ggcgtgcgcc gcggcgtcga ggcgcaacag gcgctggccg
    10201 acgtgcagcg cgcgcaactg gatgcgacct acctgacgct ggccgcgaat gtggtcaccg
    10261 catcgctgca ggaagcgtcg ctgcgcgagc aggtccgcgc gacccggcag atcatcgatt
    10321 cgctccagca ggaactcgac atcgccgaga agcagcaggc gatcgggacc acctcgctca
    10381 ccgacacctt ggccgtgcgc acccagttgg cgacggcaac ggtggcgctg ccgggactcc
    10441 gcaagcaact ggcggcgacc cggaaccagc tcgcgaccta cctcggggtg acacccgcgc
    10501 agatcgacct ccagccgatc acgctcgatg aggtgacctt gccggccgac gtcccggtca
    10561 gccttccctc gacggtggtg gcgcagcgtc cggacattcg tgccgccgcc gcacagttgc
    10621 acgccgccac cgcgggcgtc ggcgtggcga ccgccaacat gctgccgcag ctgacccttt
    10681 ccggcagcgc cgggtcgcag gcgctccacg cgggcgacct gttcaccgcc ggcaccagcg
    10741 cctggtcgat cgggctgaac atcctgcagc cgctgttcca cggcggcgag ctgctgcaca
    10801 agaagcgcgc ggcggaggcc ggcatggacc aggcgctggc gaactggcag caggtcgtgc
    10861 tggccgcttt ccaggacgtc gccgattcgc tgcaggcgct cgagtacgac gcgcattcgc
    10921 tgcgcgcgct cgcgaccgcg gaacaggacg cgtcgcgttt gctggcgctg acccgcagcc
    10981 agtaccgcgt cggggccgtc ggttacctga acctgctgaa cgccgagcag acctggcagc
    11041 aggcccgcat ttcgctgatc caggcgcgcg ccgcgcggct cgccgatacc gcggcgcttt
    11101 acgcggccct cggtggcggc tggcagcagg gcgagcccgt cgcaacaaag aagtagctcc
    11161 cggacaagac acgggcgtcg cagatccgga ggcgcccacg agacctccgc aacccactgt
    11221 cgttgagcaa ggcgccgccc gttccaccga cgccccaaaa atcaaagaga cgatcccatg
    11281 aacgcgaacc aaccctccgc ccggcccaag ctccgcaagc gactgatcat cgtgatcagc
    11341 gcggtcgtgg tgctgttcgc cctcctggtc ggtttgcacg cgttcatcgg catgaaaatc
    11401 ggcgaggcca tggcgtccat ggtcaatcca ccccagaccg tcagcaccat ggtcgcgagg
    11461 taccaggact ggcaaccgca cgtcgaggcg ttcggcaacg tgcgcgcggt gcatggcgcc
    11521 gatctcgcgt tcgacgtgtc gggcctcgtg gacagtgtcg aggtgcgctc gggcgccgac
    11581 gtaaaaaagg gcgacgtgtt gatcaagttg aacgccgacc aggatcgggc gacgctcgat
    11641 gcgctgcgcg ccagggccag cctcgccgag gtgaccgcca agcgggccag gcagcagctc
    11701 gccgtcaagg cgatcagcca ggcgagctat gacgcggccg ttgccgacct caagaacgcc
    11761 aaggcacagg ccgatgcaca ggccgcactg gtcgcgaaga agaccctgcg cgcgccgttc
    11821 gcgggacgca tcggcatcat caccgccaat ccgggccaat acatcaacgc tggcgctacc
    11881 gtggtgactt tgcagcagct cgacccgttg tacgtggatt tcaccgtgcc gcaatcgtcg
    11941 ctgaggcaga tgcaggtagg cggcgaagtc caggtcacgg ccgacgcgtt cggcgacaaa
    12001 gcgttcaagg gcgagatctc ggcgaccgat cccaaggtcg acgtgtcgac ccgcaacgtc
    12061 ggcgtcgagg ccaccgtcgc caaccccgac gaactgctgg tgccgggcat gttcgcgaag
    12121 gtcgcggtcg atgcgggccg cgagcagcgc tacctgaccc tgccgcagac gtccgttgcc
    12181 tacgcgcctt acggcgacac cgtgttcgtg gtgcacgagg ggcagccgcc ggcgcgcgac
    12241 gcgaacggca agccggtccc tgccgcatcc gccggcaaca agccggagca ggacaaggac
    12301 ggcacctcgc attacgtgca acaggtcatc gtccagactg gacccacccg cggcgaccag
    12361 gtcgcgatcc tgtccgggat caaggaaggc gacgtggtcg tgaccagcgg ccagttgaaa
    12421 ctgaagaacg gcacgccggt gaacgtcaac aacaaggtcg agccggcctt cgatccgaac
    12481 ccgcacccgg tcgaggagta ggagcgcgct atgcgcgcga gtgcggcggt cgccggcaac
    12541 agcaaaagac ggcgccgtgc gccggccggc tcctacagat ccatgaagat gcccttgcat
    12601 agactgcagc catgaaattc accgaccttt tcatcaagcg cccggtactc gccgccacgg
    12661 tcagcctgct gatcctggtg ctcggcctgc gtgcggtgac gtccctgccg atccggcagt
    12721 acccgaagac cgagagcgcg acgatcaccg tgcagaccgt ctacttcggt gccgacgcgc
    12781 aaaccatggc cggcttcatc acccagccgc tcgagcaggc gatctcgcag gcgcagggca
    12841 tcgattacct gtcctcgtcg tccacgcccg gcgtgtcgat gatcagcgcg aggctgcgcc
    12901 tcaattacga cggcaacaag gcgctcaccg agatcagcgc gctggtcaac tcggtgaaga
    12961 accagttgcc gcaggctgcg cagcagccgg tcatcacgct gcagaccggc cagcagaccg
    13021 cctcgatgta cctcgggttc tattccagca cggtggcctc caacaagatc accgactacc
    13081 tgacccgcgt ggtgcagccg cagttgaacg cggtcgacgg cgtgcagagc gcccagatcc
    13141 tgggcggccg caccttcgcg atgcgcgcgt ggctcgatcc ggtgaagatg gccgcgcacg
    13201 gggtaaccgc gaccgatgtc aacgcggcgc ttgcgtccaa caatttcctt tcggcgatcg
    13261 gcaataccaa gggccagatg gtcagcgttg cgctgaccgc ggccaccgac ctgcacacgg
    13321 tcgacgagtt caaggacctg gtgatcaagg ccgacggcac cgacatcgtg cgactgaagg
    13381 atgtcgcgcg ggtcaccttg ggcgcggaaa actacgattt caacgtcgcc ttcgacggca
    13441 agcgctcggt gttcatcggc atcaatgccg cgcccgacgc caacgtgctg gacgtgatct
    13501 ccggcgtgcg caaggtgttc ccgacgatcc agtcgcagat gcccgcgggc atgacggcgc
    13561 ggatcgtgtt cgacggcacc gactacatcc gcacttcgat ccatgaggtc gtgcagacgc
    13621 tggtcgagac gctgctgatc gtggcggtcg tgatcttcct gttcctcggg acgctgcgct
    13681 cgctgctgat cccgctggtc gcgatgccgc tgtcgttgat cggtgcgttc ctggtgatgc
    13741 tggtgctcgg ctattcgatc aacctgctga cgttgctggc gctggtgctg gcgatcggcc
    13801 tggtggtcga cgacgcgatc atcgtggtcg agaacgtcga ccgccacatg aaggagggcc
    13861 agccgccgct gcaggcggcg atcctggccg cgcgcgagtt ggccggcccg atcctcgcga
    13921 tggccgcggt gctcgtcgcg gtgtacatcc cggtcggctt ccagggcggc ctgaccggcg
    13981 cgctgttcac cgaattcgcg ttcacgctcg cgggtgcggt ggtgatctcg gcgatcatcg
    14041 cgttgacgct gtcgccgatg atgtgctcgc gctcgctgcg cgcttccgac cacgagaagc
    14101 ccttcgtcaa gcgcatcgac cgcgtcgccg atgccgtgcg cgaccgctac cggcgcacgc
    14161 tgcactcgtc gctggacacg tggctggtcg cggtggtgtt cggtgcgctg atcctgctcg
    14221 gcaacgtgta cctgttcaag acctcgcagt cggaactggc cccgcaggaa gaccagggca
    14281 tcgtgctcac caatacccag ggaccgccca acgcgaccat cgaccagatg gcgctgtatt
    14341 cgaagcaggt gttcgacatc gccaagtcgc tgccggagta cacccacatc ttccaggtgg
    14401 acggagcgca gggcgtcaac agcgggttcg ccggcatcat gctgaagcca tggggcgacc
    14461 gtgaccgcag cgcgacgcaa atccagcagg ccgtccagca gaaactcggc aacatcgcgg
    14521 gcgcgcgcgt cgcggtgttc cagttcccgt cgctgccggg cgcgttcggc ctgccggtgc
    14581 agttcgtgat caagaccacg gagccgttcc agaacttgaa cgaagtggcg aaacaggtgg
    14641 tcgcgaaggc gcaggcgtcg ggcatgttct tctacatcga ctccgatctc aagatcgacc
    14701 agccgcaggc gacggtgaca ctcgaccgca acaaggtgtc cgccctgggg ctgacccagc
    14761 gcgatgtcgg cgcggccttg tcggcggcac tgggcggcaa ctacgtgaac tacttctcga
    14821 tttcgggccg tgcctaccgg gtgatctcgc aggtcgcgca ggtggatcgc atgaacccgg
    14881 accagctcaa gaactactac atcaagacgc ccgagggcgg gatgatcccg gcttcgacgg
    14941 tggcgcacct cacgttccag acggtgccgg aatcgatcaa ccacttccag cagttgaact
    15001 ccgcgacgat ccagggcgtg atcgggcccg gcgtatcgct gggccaggca ttgcagttca
    15061 tgcaagacac cctgcaggag gtggcgccga gcggctacgc cgccgattac gcgggccagt
    15121 cgcggcagtt cgagcaggag tcgggtgggt tcttggtgac gatgctgttc gcgatcatca
    15181 tcgtgttcct ggcgttgtcg gcgcagttcg agagcttccg cgatccgatc gtgatcctgg
    15241 tgtcggtgcc gatggcgacc ttcggcgcac tgctgttcat caacctcggc ctggcgacgc
    15301 tcaacatcta caccgaggtc ggcctcgtga ccctgatcgg cttgatcagc aagcacggca
    15361 tcctgatggt gcagttcgcc aacgatgcgc agcggctgca cggcatgacg aagcgcgagg
    15421 ccatcgagga agccgcggcg gtgcggttgc ggccgatcct catgaccacc tgcgcgatgg
    15481 tgttgggcgt gatcccgctg gtggtggccg cgggcgcggg cgcggccggc cgccacagca
    15541 tcggcctggt gatcttcacc ggcatttcga tcggcaccct gttcacgctg ttcgtggtgc
    15601 cggcgatgta cctgctgctg ggccaggacc accacgccga ggcgcgcgtc cccgaacgcg
    15661 acgtgctgcc accaccgctg ccggccgagt aggcgcgttc cccgacgcca tc
//
LOCUS       DASSNH010000133         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1606552, whole genome shotgun sequence.
ACCESSION   DASSNH010000133 DASSNH010000000
VERSION     DASSNH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606552"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFK00_04905"
     CDS             complement(<1..763)
                     /locus_tag="VFK00_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592326.1"
                     /translation="MNRLGIRRGLRSSDFRLIAKEGFGDGLNSYAHTMTWFKGRLYVT
                     TMRGCFALMRLRLGLGLDVWPVDCPLDPFELDLRAEIWAYDPLEDAWERVFKAPWITG
                     SDGSRIPRDISYRAATVHRDPESGEEKLFISTWSPAKGPGPVIMCTEDGRNFKVTCEP
                     GLCGLPVTTIRSLVSFKGRLYTTPVGSRGGNTNISAHSVVYESRHPETGDWQPVSDFG
                     FGDITNLSLLECTSWGDHLYASTGNNKGFQLWRSTC"
     gene            complement(769..1824)
                     /locus_tag="VFK00_04910"
     CDS             complement(769..1824)
                     /locus_tag="VFK00_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7592327.1"
                     /translation="MPKAVINGVRLHYLQIGDPDASDCDDLVMLHGLASNLAFWYFQY
                     AQELANRFRVTLLDFRGHGRSDMPASGYSPEELSRDVGGLLDYLGIERAHFIAHSFGG
                     LVALHYARRHPAKVRSMVLADCYVPGLCSSRPAQAWAQGLKLRPILQRHGIDLDTEDP
                     RFGFRLLGVMARLRLRGEPLPPDLLCLFGPVMQRFGQNTAEQWLKLLETTTAEADITS
                     SRPMQFEELRGLGFPICAMYGDGSHARRIGDELLGAWPEAEFRLVRRAGHFFPVSRAD
                     ELLSQCKQFWDSCGNGGVHVRRPNDTRAYFQSDRFYMVDGAWCCLLREGVRLGPFPSR
                     EEAARQLTATLAALRAP"
     gene            complement(1928..>2348)
                     /locus_tag="VFK00_04915"
     CDS             complement(1928..>2348)
                     /locus_tag="VFK00_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592328.1"
                     /translation="PGAAMAGAPDLQLVLRDFGFVSVRNVEPVLEKRQYVIGTHHPDG
                     VFLGYGPGIEAGKVVARRHITDVTSTLLYSLGLPIPSDLEGKVPTAMFTAEYQAEHPV
                     KIGESTTGGNREQLSETMDDEEKQQLMDQLRMLGYME"
BASE COUNT          430 a          800 c          720 g          398 t
ORIGIN      
        1 cgcaggtgct gcgccacaac tggaatccct tgttgttgcc ggtgctcgca tagaggtgat
       61 cgccccagga cgtgcactcg agcagcgaga gattggtgat gtcgccgaag ccgaaatcgc
      121 tgaccggctg ccagtcaccc gtttcgggat ggcggctctc atagaccacc gaatgcgccg
      181 agatattggt gttgccgccg cgcgagccga ccggggtcgt gtacaggcgg cccttgaacg
      241 acaccagcga acggatcgtc gtgaccggca ggccgcacaa gccgggctcg cacgtcacct
      301 tgaagttccg cccgtcttcg gtgcacatga tcaccggccc cggccccttc gccggcgacc
      361 aggtgctgat gaacagcttt tcctcgcccg actccggatc gcggtggacc gtggcggcgc
      421 ggtagctgat gtcgcgcgga atccgcgaac catcgctgcc ggtaatccac ggcgccttga
      481 atacgcgctc ccacgcatcc tcgagcgggt cgtacgccca gatttcggcg cgcaggtcga
      541 gttcgaacgg atcgagcggg caatcgaccg gccacacatc gagccccagt cccaggcgca
      601 ggcgcatcag ggcgaagcag ccgcgcatgg tcgtcacgta caaccgcccc ttgaaccacg
      661 tcatcgtgtg cgcgtaggaa ttgaggccat cgccaaagcc ttccttggcg atcaacctga
      721 aatcggagct gcgcaagccg cgccgaatgc ccagtcgatt catgcccatc agggtgcccg
      781 caacgcggcc agggtggcgg tgagttgccg cgcggcttcc tcgcgcgatg gaaatggtcc
      841 gagccgcacg ccttcgcgca gcaaacagca ccatgcaccg tcgaccatgt agaagcgatc
      901 gctctggaag taggcacgcg tgtcgttcgg cctgcgcaca tggaccccgc cgttgccgca
      961 ggaatcccag aactgcttgc actgcgagag caattcgtcg gcgcgcgaga cgggaaagaa
     1021 atgccccgcg cgacgcacca gcctgaattc cgcctcgggc caggcgccga gcagttcgtc
     1081 gcctatgcgg cgcgcgtgcg agccgtcacc atacatcgcg cagatcggaa acccgagccc
     1141 ccgcagctcc tcgaactgca tcgggcggct ggacgtgatg tcggcttccg ccgtggtggt
     1201 ttccagcagc ttcagccatt gctccgcggt gttttgcccg aacctttgca tcaccgggcc
     1261 gaacaggcac agcaggtcgg gcggcaacgg ctccccacgc aaccgcagtc gcgccatcac
     1321 gcccagcagc cggaaaccga agcgcgggtc ctcggtatcc aggtcgatgc cgtggcgttg
     1381 cagtatcggc ctgagcttca ggccctgcgc ccatgcctgt gccggcctgc tgctgcacag
     1441 ccccggaacg taacagtccg ccaacaccat gctgcgaacc ttcgcgggat gccgacgggc
     1501 gtagtgcaag gcgaccaggc cgccgaaact gtgcgcgatg aaatgcgcgc gctcgatgcc
     1561 gaggtagtcg agcaatccgc cgacgtcgcg cgaaagttcc tccggggaat agccactcgc
     1621 cggcatgtcc gaacgaccgt gcccacggaa atcgagcaac gtcacccgga accggttggc
     1681 aagctcctgc gcatactgga aataccagaa cgcgaggttg gaagcgaggc cgtgcagcat
     1741 caccaggtcg tcgcaatcgc tcgcgtccgg gtcgccgatc tgcaggtagt gcagtcgtac
     1801 tccgtttatg acggccttcg gcatcaggaa tcactccccc gaggttttca cggatccagc
     1861 tccggtcgcg atccccggaa aggcgacgga tcgacaacgc aaccgctgtc tcatggccgg
     1921 aacgtcccta ttccatgtaa cccagcattc gcaactggtc catcagctgc tgcttttcct
     1981 cgtcgtccat cgtttcactg agttgctccc ggttgccgcc cgtcgtggac tcgccgatct
     2041 tcacgggatg ctccgcctgg tattcggccg tgaacatcgc ggtcggaacc ttgccttcca
     2101 ggtcggacgg tatcggcagt cccaggctgt acagcaatgt cgacgtgacg tcggtgatgt
     2161 gccgccgggc gaccaccttg ccggcttcga tcccgggccc gtagccaagg aacacgccgt
     2221 ccgggtgatg ggtaccgatc acgtactggc gcttttccag caccggctcg acgttccgca
     2281 ccgacacgaa gccgaagtcg cgcagcacca gttgcaggtc aggcgcaccc gccatcgcgg
     2341 cacccggg
//
LOCUS       DASSNH010000134         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1050887, whole genome shotgun sequence.
ACCESSION   DASSNH010000134 DASSNH010000000
VERSION     DASSNH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050887"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VFK00_04920"
     CDS             <1..1302
                     /locus_tag="VFK00_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7592329.1"
                     /translation="ELAAMVPVSGSAYTYTYGVMGELIAWIVGWALVLEYAIGATAVC
                     VGWSGYVNGLLNSGVFGLVEPGALSLPHWLQVGPTLGGGFNLLAFLIGLAVTGLLVIG
                     TSKSARVNAVLVAIKIIALTVFIIIAVPAVKNANLHPFLPTGWGNPVGSNGIGVLGAA
                     ASIFFAYVGFDAVSTAAEETRNPNRNVPIGLIAALVICTIFYLLVGYGAAGSVGAQPL
                     TTAAGAVLDPGTPAFAAACGNSDALVCSREPLAQVMSTLGHPRVAVMIELAAIVALPS
                     VILMMMFGQTRIFFTMSRDGLLPNMFSKVHPRFFTPHVITYVTGAVVSLCAALFPVGK
                     LADTSNAGTLLAFAMVSVGVMVLRRTQPGRKRPFRTPLVWFVCPLAVVGCLLLFVNLS
                     VVAKVVFLVWAVIGLIFYRLYGYKRSQMSPEQITHALDDVV"
     gene            1403..2848
                     /locus_tag="VFK00_04925"
     CDS             1403..2848
                     /locus_tag="VFK00_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7592330.1"
                     /translation="MLTRLLARKPVDDTGGPEIAGGIELRRTLGPWGLTALGIGAVIG
                     GGIFVITGQAAAEHAGPAVLLSFVIAAVVCSFTALCYAEFATLIPMSGSSYSYAYATL
                     GEFAAWFIGWNMVLEYGVSASAVAVSWTGYFRSLVRHMGFNLPDALSNAPIAFVDKHL
                     VVTGALFNLPAVVLVLALTWLCYVGIRESSGVNTAIVILKVTLIVIVIVAGWHYVNPD
                     LWHPFIPPAEGPEKYGWGGIMRAATLVFFAYIGFEATSTAAQEARNPQRDLPIGTLAS
                     LAICTVLYVAMAAVLTGLIPFAQLGTAEPVVTAVAAHAELNWLRWLVEIGALLGLSSV
                     VLVMIIAQPRIFMIMGRDGMLPPVFSKLHPRYRTPHINTLITGTGIALLAAIFPLDVL
                     GDLVSMGTLLAFVAVCAGVLILRYTRPDLPRTFRVPWAPLTCALGVLGCIVLLCYENW
                     YNWTLMGLWTLIGFAIYFGYGYHHSRLKGRD"
BASE COUNT          479 a         1032 c         1047 g          636 t
ORIGIN      
        1 gaactcgcgg caatggtgcc cgtttcggga tccgcataca cgtataccta cggcgtgatg
       61 ggcgaactga tcgcctggat cgtcggctgg gcgctggtgc tggaatacgc catcggcgcg
      121 acggccgtgt gcgtcggctg gtccggctac gtcaacggct tgctcaacag cggcgttttc
      181 ggcctggtcg aaccgggtgc attgtcgctt ccgcattggc tgcaagtcgg gccaacgctc
      241 ggtggcggat tcaacttgtt ggcgttcctg atcgggcttg ccgtgaccgg cctgttggtg
      301 atcggcactt ccaagagcgc tcgcgtgaac gccgtgctgg tcgcgatcaa gatcatcgca
      361 ttgaccgtgt tcatcatcat cgccgtaccg gcggtgaaga atgccaacct gcatccgttc
      421 ctgccgactg gctggggcaa tccggtcggc agcaatggca tcggggtgct gggtgcggcg
      481 gcatccatct tcttcgccta cgtgggattc gacgcggttt ccaccgccgc agaggagacc
      541 cgcaacccca atcgcaacgt tcccatcggg ctgatcgccg cattggtgat ctgcaccatt
      601 ttttacctgc tggtgggcta tggcgcggcc ggttcggttg gcgcacaacc actcacgact
      661 gcggccggcg cagtcctgga cccgggcacc cccgcattcg cggcagcgtg tggcaattcg
      721 gatgcgctgg tgtgcagccg cgaaccgctg gcgcaagtga tgagcacgct cggacatccc
      781 agggtcgccg tcatgatcga gctcgcagcg atcgtggcgc tcccgtcggt gatcctgatg
      841 atgatgttcg gccagacccg catcttcttc acgatgtcac gcgatggcct gctgcccaac
      901 atgttctcga aagtgcaccc gcgcttcttc acgccacacg tgatcaccta cgtcaccggc
      961 gccgtggtat cgctgtgcgc cgcgttgttc ccggtcggca aactcgccga cacatcgaac
     1021 gccggcaccc tgctcgcctt cgcgatggtc tcggtgggcg tgatggtcct gcgtcgcacc
     1081 caacccggcc gcaagcgccc cttccgcacg ccgttggtgt ggttcgtgtg cccgttggcc
     1141 gtcgtcggct gcctgttgtt gttcgtcaac ctcagcgtcg tggccaaggt ggtgttcctg
     1201 gtgtgggccg tgatcgggct gatcttctat cggctgtacg gctacaagcg cagtcagatg
     1261 tcgcccgaac agatcacgca tgcgctggat gacgtggtgt gacgatgccg ggtgatcaca
     1321 tggcggctgg ccggtcgccc aggcacgacg tcggccgccc ccgcatcgcg aggccagcac
     1381 gggtcggcgg ccggaaaccg gcatgctgac gcggctgctc gcacgcaaac cggtcgatga
     1441 caccggcggg ccggagatcg cgggcggcat cgagttgcgt cgcacactgg ggccatgggg
     1501 cctgaccgcg ctcggcatcg gtgccgtcat cggcggcggg atcttcgtaa tcacaggaca
     1561 ggccgcggcc gagcacgccg ggccggcggt actgctctcg ttcgtgattg ccgccgtggt
     1621 ttgcagcttc actgcgttgt gctatgcgga gttcgcgacg ctgatcccca tgtcgggcag
     1681 ttcgtattcc tacgcctacg ccacgcttgg cgaattcgcc gcgtggttca tcggctggaa
     1741 catggtgctg gaatacggcg tctcggcgtc ggcggtggcg gtcagctgga cgggctactt
     1801 caggagcctg gtccggcaca tgggcttcaa tctgcccgac gccctgagca acgcgccgat
     1861 cgcgttcgtg gacaagcacc tcgtggtgac gggtgcgctt ttcaacctgc ccgccgtggt
     1921 gctggtgctg gctctcacgt ggctttgcta cgtcggcatc cgtgagtcca gcggcgtcaa
     1981 caccgcgatc gtgatcctga aggtcacgct catcgtcatc gtgatcgtcg cggggtggca
     2041 ctacgtcaat ccggacctgt ggcatccgtt cattccgccg gcggaagggc cggagaagta
     2101 cggctggggc ggcatcatgc gcgccgcgac attggtcttc ttcgcctaca tcggcttcga
     2161 ggcgacctcg accgccgcgc aggaagccag gaacccgcaa cgcgacctcc cgatcggcac
     2221 gctggcatcg ctggccatct gcactgtcct gtacgtggca atggccgcgg tgctgaccgg
     2281 gctgatcccg ttcgcgcaac tcggaacggc cgaaccggtt gtcaccgcgg tggccgcgca
     2341 cgccgaattg aactggctgc gctggctggt ggagatcggc gccctgctgg ggctgtcgtc
     2401 cgtggtgctg gtgatgatca tcgcgcagcc gcgcatcttc atgatcatgg gccgcgacgg
     2461 catgcttccg ccggtgttct ccaagctgca cccacgctat cgcactccgc acatcaacac
     2521 gctgatcacc ggcaccggca ttgcgctgct ggcggccatc ttcccgctcg atgtgcttgg
     2581 cgacctggtc tcgatgggca cgctgctggc atttgtcgcg gtgtgcgcgg gcgtgctgat
     2641 cctgcgctac acgcgccccg acttgccgcg cacgttccgc gtaccgtggg cgccgctgac
     2701 ctgcgctctc ggggtgctcg gctgcatcgt tctgctgtgc tacgagaact ggtacaactg
     2761 gacgctgatg gggctatgga cgctgatcgg attcgcgatc tatttcggct acgggtacca
     2821 ccacagccgg ctgaagggtc gcgattgatc ggacggctcc gcgcaacagc cgagaacaca
     2881 atgcaggagc cgtcgcggcg cggcgtttgc tcggtattct gcgcgcccgt gctgctggcg
     2941 acgcttattg cagggcgggg cttgctcctg cccggttaag aatccaaaag cgcgccatcc
     3001 gcgcaacagg cgccacgaca aacgctgatc gcgggcatgg cccgcttcga caacagctcg
     3061 gagaatgcag aagcgcgtca tgcacgcgac ggaacgctgc acgtgtggat cggcgccatt
     3121 ggggggcgat ttcgtcagcg cagaggcgcc gccctccaag tccgcgccga gctgaccata
     3181 cgcaaccgca ttgt
//
LOCUS       DASSNH010000135         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1709842, whole genome shotgun sequence.
ACCESSION   DASSNH010000135 DASSNH010000000
VERSION     DASSNH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709842"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /gene="yidC"
                     /locus_tag="VFK00_04930"
     CDS             complement(<1..1357)
                     /gene="yidC"
                     /locus_tag="VFK00_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7592331.1"
                     /translation="MINQTRTLLVVALLVVAYLLWQGWQNDYPSAAPAPAPASSAVPS
                     SAAAPVAPGTATVPEVPAASTAAAAQPAAPGRAAATQVPQGAANPAATPGKDLITVDN
                     DVLRLTIDPRGGTIVAADLLDYPQHVKAGSPPVRLLDGAAGRYYVAQSGLVGVDGDAA
                     PSHLAEFTATATHYSLTPGQKQVVVTLDWRDRASGIIVAKRYTLTRGSYVVAMSQEID
                     NHGAKTWRGNAYEQLDRIEPPPPPKHLLEFYNPDSLSYTGAAWYGPDDKFQKLKFEDF
                     QEDPLNKQVSGGWVAMSQRFFVSAWIPPAQQAENFSSVEIPASTTDGKPRYLIRSMGP
                     TITVAPGKSATSSARLYVGPKLQNQLADIAPGLNLTVDYGMFTVIAAPMFKLLSFLHW
                     LVHNWGLAIVVLVLLIQAATYKLTEAQYKAGAKMRKLQPRLKALKERFGDDKQKYQQA
                     MM"
     gene            complement(1401..1775)
                     /gene="rnpA"
                     /locus_tag="VFK00_04935"
     CDS             complement(1401..1775)
                     /gene="rnpA"
                     /locus_tag="VFK00_04935"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7592332.1"
                     /translation="MAGLPPSARLRRAAEFAAFRTASGKVQTRHFLVRMLPSAAGYPR
                     LGLAVSRKVSKRAVARNRIKRIIRESFRVRRAALPGVDVLVIARPSAAATDNPSLRAD
                     LEVAWQKLAALMAKPAPGTMGG"
     gene            complement(1785..1919)
                     /gene="rpmH"
                     /locus_tag="VFK00_04940"
     CDS             complement(1785..1919)
                     /gene="rpmH"
                     /locus_tag="VFK00_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7592333.1"
                     /translation="MKRTYQPSVLKRARTHGFRARMKTADGRKVLSARRAKGRKRLIP
                     "
     gene            2124..3440
                     /gene="dnaA"
                     /locus_tag="VFK00_04945"
     CDS             2124..3440
                     /gene="dnaA"
                     /locus_tag="VFK00_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7592334.1"
                     /translation="MSDLWQRCLARLEGEIEPSVLHTWLQPLQARDDDGTLRLFAPNS
                     YTLEFVRSEYLARIEGTLSALAGRPVPLRLEVGSVTAAPRASAPAGTVPAAAPAQRHN
                     LDPHYTFDNFVEGKSNQLGKAAAMQVAQNPGRAYNPLLLYGGTGLGKTHLMQAAGNLM
                     LAHNPSMKLLYVHSERFTEMMITSLRNKNMDDFKHRFRSVDALLIDDIQFFAGKDATQ
                     EEFFHTFNTLYESRQQIVLTCDRYPKEVDNLELRLKSRLGWGLSVAIEPPDFETRAAI
                     LLEKAQARGIPLPSDVALLLARRIRSNVRELEGALNTVAAQANLSGRGITMELVQETL
                     RDLFNVHARAITIQNIQKTVADYYQLRLSDLLSQRRVRSLARPRQIAMALAKELTEHS
                     LPEIGEAFGGKDHTTVMHACKKIRSLCDTDMRLRQDWDKLIRILTG"
     gene            3648..4748
                     /gene="dnaN"
                     /locus_tag="VFK00_04950"
     CDS             3648..4748
                     /gene="dnaN"
                     /locus_tag="VFK00_04950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7592335.1"
                     /translation="MRFSTQRESLLKPMQQVVGVVERRQTLPVLANLLVQVKGGKLSL
                     TGTDLEVEMRAGAVVDAGEDGETTIPARKWFDLVRALPDGVKVDVQVKGDRVVMHAGR
                     SRYTLASLPADEFPTTDEIEVTDRIALPEGNLRRLLERTSFAMANQDVRYYLNGLLLD
                     LRGSELRCVATDGHRMAMAECALEGTAKGKRQLIVPRKGVTELLGLFESSEDPVNIEF
                     SANHIRVRRGDVTFTSKLIDGSFPDYEAVIPLGADKIVRVDREAIRNALQRAAILSNE
                     KYRGVRLEVSPNCLRVIAHNPEQEEAFEEIEAETPAGDIAIGFNVGYLLDALGALDED
                     QVHLQLRDAQSSCLLRGAASEQTQHVIMPLRL"
     gene            4786..>5343
                     /locus_tag="VFK00_04955"
     CDS             4786..>5343
                     /locus_tag="VFK00_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7592336.1"
                     /translation="MNINLLRASNLRCFERFEFVPSSGINWLVGPNGAGKTSVLEAAY
                     ILSHGRSFRAGGRGAPGRHGTHEYLVHAELSRKDQARQRIGLRRGRDRWQARLDGADL
                     TTLAPLFASCPVVYFGPESQSLIVGPSETRRSFLDWSVFHVEHDSLQAWRIWRRALRQ
                     RNALLRTGAPAAEFEPWEHDLAKLAA"
BASE COUNT          925 a         1712 c         1789 g          917 t
ORIGIN      
        1 ccatcatcgc ctgctggtac ttctgcttgt cgtcgccgaa gcgctccttg agcgccttca
       61 ggcgcggctg cagcttgcgc atcttcgcgc ccgccttgta ctgcgcctcg gtgagcttgt
      121 aggtcgccgc ctggatcagc agcaccagca cgacgatcgc aaggccccag ttgtggacca
      181 gccagtgcag gaacgacagc agcttgaaca tcggcgccgc gatcacggtg aacatgccgt
      241 aatcgacggt gaggttcagg ccgggtgcga tgtcggcgag ctggttctgc aacttggggc
      301 cgacatagag ccgggcgctg ctggtcgcgc tcttgcccgg ggcgaccgtg atggtcggcc
      361 ccatgctgcg gatcaggtaa cgcggtttgc cgtccgtggt cgacgcgggt atttctacgc
      421 tgctgaagtt ctccgcctgc tgggcgggcg gaatccaggc ggacacgaaa aaccgctggc
      481 tcatcgcgac ccagccgccg gacacctgct tgttcagcgg gtcttcctgg aaatcctcga
      541 acttgagctt ctggaatttg tcgtccggcc cgtaccacgc ggcgccggtg tagctcaggc
      601 tgtcggggtt gtagaactcg agcaggtgct tgggcggcgg cggcggttcg atccggtcga
      661 gttgctcgta ggcattgccg cgccaggtct tcgcgccgtg gttgtcgatc tcctggctca
      721 tcgcgaccac gtagctgccg cgggtaagcg tgtagcgctt ggccacgatg atgccggagg
      781 cacggtcgcg ccaatccaac gtgaccacca cctgcttctg tcccggcgta agcgagtagt
      841 gcgtcgcggt cgctgtgaac tcggccaggt ggctgggcgc cgcgtcgccg tccacgccga
      901 ccagcccgct ctgcgcaacg tagtagcgcc cggcggcgcc atcgagcagc cgcaccggcg
      961 ggctgcccgc cttgacgtgt tgcgggtaat ccagcagatc ggcggccacg atggtgccgc
     1021 cgcgcggatc gatcgtcagc cgcagcacgt cgttgtcgac ggtgatcagg tccttgcccg
     1081 gcgtcgcggc aggattggcc gcgccttgcg gcacctgcgt ggcagcggcg cggcctggcg
     1141 ccgccggttg cgccgcggca gcggtcgagg cagccggcac ctccggtacg gtcgcggtac
     1201 ccggcgccac gggcgccgcg gcagacgacg gaaccgccga gctggcgggc gccggcgcgg
     1261 gcgccgcgga cggatagtcg ttctgccagc cttgccagag caggtaggca acgacgagca
     1321 acgccacgac cagcagtgtg cgtgtttgat tgatcacgaa agggggactc ggacttgggg
     1381 cgcgctgtgg ccggagcggg tcagccgccc attgtgccgg gtgcgggctt cgccatcaac
     1441 gcggccagct tttgccatgc gacctcgaga tccgcgcgca gcgaggggtt gtcggtcgcc
     1501 gcagcggacg gacgcgcgat caccagcacg tccacgcccg gcagggccgc gcgtcgcaca
     1561 cggaacgatt cgcggatgat ccgcttgatg cgattgcgcg cgacggcacg cttgctgacc
     1621 tttcgcgaaa ccgccaggcc cagccggggg tatccggcgg cggacggaag catgcgcacg
     1681 aggaagtgcc gcgtctggac ctttcccgaa gccgttcgaa aggctgcgaa ttcggcggcc
     1741 cggcgcagcc gcgcggaagg cggcaagccg gccacgcgaa gcgcttacgg gatcagccgc
     1801 ttgcggccct tggcgcggcg tgccgagagt accttgcgcc cgtcggcggt cttcatgcgc
     1861 gcgcggaaac cgtgggtacg ggcccgcttc agcacgctgg gctggtaggt acgtttcatg
     1921 gctggctccg cagcggaatg cccgcgcgag cgggaattgc atggaaagga ccgcgaattc
     1981 tacggggtcg cacggctgcg cgtcaacgtg gccgctgatg gtatctcgtg ctccggatcg
     2041 acggctacgc gcttgggcag cctgctagac tcggctgttc accggttgca ggctgcgacc
     2101 gggtcgatac acgacgggaa tccatgagcg acctctggca acgctgcctc gcaaggctcg
     2161 agggggaaat cgagccgagc gtgctgcaca cctggctgca gccgctgcag gcgcgcgacg
     2221 atgacggcac cttgcggctg ttcgcaccga acagctacac gctggaattc gtgcgaagcg
     2281 agtacctcgc gcgcatcgaa ggcacgctgt cggcgctcgc gggccggcct gtcccgctgc
     2341 ggctggaagt gggctcggtg accgccgcac cccgcgcgag cgcgccggcc ggcaccgtac
     2401 cggcggctgc gccggcgcag cgacacaacc tcgacccgca ctacaccttc gacaacttcg
     2461 tcgagggcaa gtccaaccag ctcggcaagg ccgcggcgat gcaggtcgcc cagaatcccg
     2521 ggcgggccta caacccattg ctgctgtacg gcggcacggg tctcggcaag acccacttga
     2581 tgcaggcggc cggcaacctg atgctcgcgc acaatccgtc gatgaagctc ctctacgtgc
     2641 attccgagcg cttcaccgaa atgatgatca cgtcgttgcg caacaagaac atggatgatt
     2701 tcaagcaccg ctttcgttcg gtggacgcgc ttctgatcga cgacatccag ttctttgccg
     2761 gcaaggacgc gacccaggaa gagttcttcc acaccttcaa caccctttac gaatcgcgcc
     2821 agcaaatcgt gctgacctgc gaccgctatc ccaaggaagt cgacaatctc gaactgcgcc
     2881 tgaaatcgcg gctcggctgg ggcctgtcgg tcgcgatcga gccgccggat ttcgaaaccc
     2941 gcgccgcgat cctgctggaa aaggcgcagg cccgtggcat tccgctgccg agcgacgtgg
     3001 cgctgttgct ggcccgccgg atccgctcca atgtccggga actcgagggc gcgcttaaca
     3061 ccgtcgccgc gcaggccaac ctctcgggcc gtggcatcac gatggagctg gtgcaggaga
     3121 cgctccgcga cctcttcaac gtccacgccc gcgcgatcac gattcagaac atccagaaaa
     3181 ccgtggccga ctactaccag ttgcggctct cggacctact ctcgcagcgg cgggtgcggt
     3241 cgctggcccg gccgcgccag attgcaatgg cgttggccaa ggaattgacc gaacatagcc
     3301 tgccggagat cggggaggcc ttcgggggca aggaccacac caccgtgatg cacgcctgca
     3361 agaagatccg cagtctctgc gacaccgaca tgcggctgcg ccaggactgg gacaaattga
     3421 tcaggatatt gaccgggtaa acttgtggat agattctgga acactgcgga tccggaaatc
     3481 atccacaggc catgcaccgg tgttgcaaag cggtgccaag cgtgtaaatt tttccaagca
     3541 tttgattcta ataatgtttc atgtgtgtag atgttattca cacggcctgc ttctacgatc
     3601 ttatctacaa gtactaccaa tacagataac agacacgggg atcgacaatg cgcttcagca
     3661 cccagcgtga atccttgctc aagcccatgc aacaggtcgt gggcgtggtg gagcgccgcc
     3721 agaccctgcc ggtactggcc aacctgctgg tgcaggtgaa gggcggcaag ctgtcgctga
     3781 cgggcaccga cctcgaagtc gaaatgcgtg ccggcgcggt ggtggatgcg ggcgaggatg
     3841 gcgagacgac cattcccgcg cgcaagtggt tcgacttggt tcgcgccttg ccggacgggg
     3901 tcaaggtgga cgtgcaggtg aagggcgacc gggtggtgat gcatgccggg cgcagccggt
     3961 acaccttggc gagcctcccc gcggacgaat ttcccacgac cgacgagatc gaggtcaccg
     4021 acaggattgc cttgccggaa ggaaaccttc gccggctcct cgaacgtacg tcgtttgcaa
     4081 tggccaacca ggacgtgcgg tattacctca acgggctgct gctggatctg cgcgggagcg
     4141 agttgcggtg cgtggcgacc gacggccacc gcatggcgat ggccgaatgc gcgcttgaag
     4201 gcactgccaa gggcaagcgc caattgatcg tgccccggaa aggcgtgacc gaactgctgg
     4261 ggctgttcga gtcctccgag gatccggtca acatcgaatt ctccgccaac catatccggg
     4321 tccggcgcgg cgacgtgacc ttcacatcca agctgatcga cggaagcttt cccgattacg
     4381 aggcggtgat tccgctgggc gcggacaaga tcgtgcgggt cgaccgggaa gccatccgca
     4441 acgccctgca gcgtgcggcg atcctgtcca atgaaaaata ccggggcgtc cggctcgagg
     4501 tcagtcccaa ctgcctgcgc gtgatcgcac acaaccccga acaggaagag gccttcgagg
     4561 agatcgaggc ggagacgccg gccggcgaca tcgcgatcgg gttcaacgtg ggttacctgc
     4621 tcgatgcgct cggcgcgctc gacgaagacc aggtgcacct gcagctccgc gacgcacaat
     4681 ccagttgttt gctgcgcggg gccgccagcg agcagaccca gcacgtgatc atgccgttgc
     4741 ggctttgatc catggcgggc cgcccggtcc ggtgggaacg ccgcggtgaa catcaacctg
     4801 ctgcgcgcct cgaacctgcg ttgctttgaa cgtttcgagt ttgtcccctc gagcgggatc
     4861 aactggctcg tcggccccaa cggcgccggc aagaccagcg tgctggaggc cgcctacatc
     4921 ctgtcgcatg gtcgttcatt ccgggcgggt ggccgggggg cgcccggccg gcatggcacg
     4981 catgaatacc tcgtccatgc cgagctttcg cgcaaggacc aggctcggca gcggatcggc
     5041 ttgcgccggg ggcgggatcg ctggcaggca cgcctcgatg gcgccgacct caccaccctt
     5101 gccccgctgt tcgccagttg cccggtcgtg tatttcggtc ccgagagtca atcgctgatc
     5161 gtagggccat cggaaacgcg gcgaagcttc ctggattgga gtgtgttcca cgtggaacac
     5221 gattcgctgc aggcttggcg gatttggagg cgggccctgc ggcagcgcaa tgcgctgctg
     5281 cgcaccgggg ccccagctgc ggaattcgag ccgtgggagc acgacctcgc aaagctggcg
     5341 gca
//
LOCUS       DASSNH010000136         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1139972, whole genome shotgun sequence.
ACCESSION   DASSNH010000136 DASSNH010000000
VERSION     DASSNH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139972"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFK00_04960"
     CDS             complement(<1..615)
                     /locus_tag="VFK00_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592337.1"
                     /translation="MTSSSERAMRAVGLPLAVLAIVLALAGALAIARQAAAQVVHPVV
                     STSALTEQDFHRILPKLVAQTPLAKSDRTDPQADALPARALTTEDFKRMLPRTDMRSD
                     ADANAHPRVHAWLDSGPATPFDAAAIAKNPLAPDAIAIGAKSCVACHSLENVHASHSL
                     HVQAFRADISGAGAAAACEACHGPGSAHAKDPTQPGLIIAFTRDA"
     gene            complement(599..1630)
                     /gene="folE2"
                     /locus_tag="VFK00_04965"
     CDS             complement(599..1630)
                     /gene="folE2"
                     /locus_tag="VFK00_04965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HET7592338.1"
                     /translation="MTSADAPLALADVAPASGTTSGHHPAATAEDPAPDVADEMLPRH
                     EAAIDWVGMQGIAMPILFDAGDGNLQRCHARVDVFVNLARPDRRGIHMSRLYLLASRH
                     LDDEPLRAGMLEALLRALVRSHEGLADRARIRIHFEYLVRRRALRTHHHGWRAYPVVV
                     EAVLIGDAFHLELATQVVYSSTCPASAALSRQLIQRQFAADFPAGESVDHAAVLAWLG
                     ARGIVATPHAQRSVAQVRVRLAHEAPLNLAGLLERIEGSLRTAVQTAVKREDEQAFAL
                     ANGENPMFCEDAARRIRRVLDADADVDAYHVRVEHQESLHPHDAVAQVHRGLPGSAHA
                     ARIEHDFFV"
     gene            complement(1623..2933)
                     /locus_tag="VFK00_04970"
     CDS             complement(1623..2933)
                     /locus_tag="VFK00_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7592339.1"
                     /translation="MKHGALLLAAAAIAWCAAAHARSVAPPGGSFAAQTVPAKGAAAL
                     DARRDIVLAVADPLAPPAMHAGSSLLGYVPASHYGGAGMRAALTLTALKQRYGWRELE
                     GWPIKSLGLYCIVLEPPGGADRAALLKQLAADDRVRLAEPLRSFSVYSGTAPAPHQYN
                     DPYVDLERGFIDTAAARAHDWSQGKGVDVAVVDTGADTTHPDLHGQIRGAYNEVDEDA
                     GAFNGDHHGTEVAGIIAAIANNHVGIVGMAPKATLSIYKACWYPPEAAGARCNSFTLA
                     KALAAVLDNTDARVVNLSLGGPADPLLAMLLRQLLGQGRIVVAAMPPDGDLGGFPDEA
                     PGVIVVRTTGASTAPPGVLSAPGRDILTTQPDDGYDFSSGSSMAAAHVSGIAALLVSL
                     APGLDARTIHDLLLRSSETVDGTLQVNAAAAVAALRNQEASLHD"
     gene            complement(2930..3562)
                     /locus_tag="VFK00_04975"
     CDS             complement(2930..3562)
                     /locus_tag="VFK00_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7592340.1"
                     /translation="MTIETNSGKDCTRAWEVMPWVLQAAAPREQGEWLMHHLAQCEAC
                     SNEFAQQSRLRSALALPMDVPVDVDSGLKHLLERLDAPESEVLPAQQRHVSRLTRALA
                     VAVLVQAIGIGVLGLRLWSDQAPQYRTLSQPAAIEPAGAIRVAPSTSMTVANWNELLH
                     DFDLRVVDGPNAVGAYTVVPKASAAQSAQLLQALRASRSIRLAEPVDDTP"
     gene            complement(3559..4143)
                     /locus_tag="VFK00_04980"
     CDS             complement(3559..4143)
                     /locus_tag="VFK00_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7592341.1"
                     /translation="MTEARDEADSRDRLLLQRMAAGDRAALEALYRAYHGGLARFLSR
                     LTRRADLIEEVINDCFWIAWRKADGFHGDSRVSTWLMGIAYRCGLKALRQRGDEPVDD
                     YALREDRGPSHDPDEDRQLRDWLGKALEHLSADQRVVVELVYGVGNSLEEVAAIMQAP
                     VGTVKARLFHARIKLRNVLPALAGETPAQREKHS"
     gene            complement(4246..>4623)
                     /locus_tag="VFK00_04985"
     CDS             complement(4246..>4623)
                     /locus_tag="VFK00_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592342.1"
                     /translation="DADVYEPGLYWLYRDVLTFGVSGIHVVGTEGHELHPAADLNFHW
                     PLGRNFALSAGLGIARYAVPYGHSAHERYHTGYYRYGQAGLIWSQGAWQVELDRVVVD
                     SGAHGYLEGRVASPWLATISRSF"
BASE COUNT          722 a         1535 c         1648 g          718 t
ORIGIN      
        1 ggcgtcgcgg gtgaaggcga tgatcaggcc cggttgggtc ggatccttcg catgcgcgga
       61 gcccggtccg tggcaggctt cgcaggccgc cgccgcgccc gctcccgaga tgtcggcgcg
      121 gaacgcctgc acatgcagcg agtgcgacgc gtgcacgttt tccagcgaat ggcaggcgac
      181 gcaggatttg gctccgatcg cgatcgcatc tggcgccaag ggattcttcg cgatcgcggc
      241 ggcgtcgaag ggggtggctg gtccgctgtc gagccaggca tggacgcgcg gatgtgcgtt
      301 cgcatccgcg tcgctgcgca tatccgtgcg cggcaacatc cgcttgaagt cctcggtggt
      361 caacgcacgc gccggcagcg catcggcctg tggatcggtc cggtccgatt tcgccagggg
      421 cgtttgcgcg acgagcttcg gcaggatgcg gtggaaatcc tgctcggtca gcgcgctggt
      481 cgaaacgacg ggatggacga cttgcgcagc cgcttgtcgg gcaatggcca gtgcaccggc
      541 gagggcgagc acgatcgcca gcaccgccaa tggcaggccg acggcgcgca tcgcccgttc
      601 agacgaagaa gtcatgctcg atccgggcgg cgtgcgcgga tcccggcaga ccccggtgca
      661 cctgcgcaac cgcgtcgtgg ggatgcaggc tttcctggtg ctcgacacga acgtggtaag
      721 cgtcaacgtc cgcgtcggcg tcgagcacgc gccggatgcg ccgcgccgcg tcctcgcaga
      781 acatcgggtt ttcgccgttg gcgagcgcga aagcctgctc gtcctcgcgc ttgaccgccg
      841 tctgcacggc tgtccgaagc gatccctcga tccgctcgag caggccggca aggttcaacg
      901 gcgcttcgtg cgcgagccgc acccgtacct gcgcgacgct gcgctgcgcg tgcggtgtgg
      961 cgacgatgcc gcgcgcgccg agccaggcca gtacggcggc gtggtccacc gattcgcccg
     1021 ccggaaagtc cgcggcgaac tgcctctgga tcagttggcg cgacagcgcg gcggaagccg
     1081 ggcaggtcga ggaatacacg acctgcgtgg cgagctccag gtggaaagcg tcgccgatga
     1141 ggacagcctc gacgacgacc gggtaggcgc gccagccgtg gtgatgcgtg cgcaaggccc
     1201 tcctgcgtac aaggtattcg aaatggatcc gtatgcgcgc gcggtcggcc aaaccctcgt
     1261 gcgatcgcac caaggcgcgc agcagtgcct ccagcattcc cgcgcggagg ggttcatcgt
     1321 cgaggtgccg actcgccaac aggtacaggc gcgacatgtg gataccgcgc cggtccggac
     1381 gcgccaggtt cacgaagacg tcgaccctgg cgtggcagcg ctgcaggttg ccgtcgccgg
     1441 catcgaacag gattggcatc gcgatgccct gcatgcctac ccagtcgatc gcggcctcgt
     1501 gccgcggcag catttcgtcg gcgacgtcgg gcgccgggtc ttcggcagtg gcggcggggt
     1561 ggtggccact cgtagtcccc gatgccggtg ccacatcggc cagcgccaac ggagcatccg
     1621 cgctagtcat ggagcgaagc ctcctggttg cgcagggctg ccaccgccgc cgcggcattc
     1681 acctggagcg tcccatcgac ggtctcgctg ctgcgcagca ggaggtcgtg gatcgtgcgc
     1741 gcgtccaggc ccggcgccag cgataccagc agcgcggcga tgccgctgac gtgcgcggca
     1801 gccatggacg atccggagct gaagtcgtag ccgtcgtcgg gctgggtcgt gaggatgtcg
     1861 cggccgggcg cgctcaacac accgggcggc gccgtcgatg ccccggtggt gcgcacgacg
     1921 atgaccccgg gcgcctcgtc cgggaatccg ccgagatcgc cgtcgggtgg catcgccgcc
     1981 accacgatgc gaccctgccc cagcaattgc cggagcagca tcgcgagcag cggatcggcc
     2041 ggcccgccga ggctcaagtt gacgacgcgg gcgtcggtgt tgtccaggac cgccgccagc
     2101 gccttggcga gcgtgaagga gttgcagcgc gcgccggccg cctcgggcgg ataccagcaa
     2161 gccttgtaga tgctgagcgt ggccttgggg gccatgccaa cgatgccgac gtggttgttg
     2221 gcgattgcgg cgatgatgcc ggcgacttcg gtaccgtggt ggtcgccgtt gaacgcgccc
     2281 gcatcctcat cgacctcgtt gtaggcgccg cggatctggc cgtgcaggtc gggatgggtg
     2341 gtgtcggcgc cggtatccac gaccgcgacg tcgacgccct tgccctggct ccagtcgtgc
     2401 gcccgggcgg cggcggtatc gatgaagccg cgctcgaggt cgacgtaggg atcgttgtac
     2461 tggtgcggtg ccggcgcggt gccggaatag accgagaaac tgcgcagcgg ttcggcgagg
     2521 cggactcggt catcggccgc gagttgcttg aggagggccg cgcggtccgc gccgcctggg
     2581 ggttccagca cgatgcagta gaggcccagc gacttgatcg gccagccctc caattcgcgc
     2641 cagccgtagc gttgcttcag cgccgtgagc gtgagcgccg cgcgcatgcc ggcgccgccg
     2701 tagtggctgg cgggaacata gccgagcaag ctggagcccg cgtgcatcgc cggtggcgcc
     2761 agcgggtcgg cgaccgcgag cacgatgtca cggcgtgcgt cgagcgccgc cgcgcctttc
     2821 gccggaacgg tctgggcggc gaagcttcca ccaggcggtg ccacggaccg cgcgtgcgcc
     2881 gccgcgcacc aggcgatggc cgcagcggcg agcagaaggg cgccgtgttt catggcgtat
     2941 cgtcgacggg ctccgcgagg cggatgctgc gcgaggcgcg caacgcctgg agaagctgcg
     3001 cggactgcgc ggcggacgcc tttggtacga cggtgtacgc gccgacggcg ttcggtccat
     3061 cgaccacccg caggtcgaaa tcgtgcagca attcgttcca gttggccacc gtcatcgatg
     3121 tcgatggcgc gacgcgaatc gcgcccgccg gttcgatcgc cgcaggctgg ctgagggtcc
     3181 ggtattgcgg ggcctggtcc gaccacagcc tcagtccgag cacgccgatc ccgatcgcct
     3241 gcaccagcac cgcgacggcc agggcccggg tcaagcggct gacgtggcgc tgctgtgcag
     3301 gcagtacttc ggattccgga gcgtcgaggc gctcgagcag gtgcttcagc cccgagtcga
     3361 cgtcgaccgg aacgtccatc ggcaacgcca gggcgctgcg caagcggctc tgctgcgcga
     3421 attcattgct gcacgcttcg cattgcgcga ggtggtgcat caaccactcg ccctgctcgc
     3481 gcggcgccgc ggcctgcagc acccagggca tgacttccca ggcgcgggtg caatccttgc
     3541 cggaattcgt ctcgatggtc atgaatgttt ctctcgttgc gcaggcgttt cacccgcgag
     3601 cgccggaagc acgttgcgca acttgatgcg cgcgtggaac aaacgtgcct tcacggtgcc
     3661 gaccggcgcc tgcatgatgg ccgcaacttc ctccaggctg ttgccgaccc cgtaaaccag
     3721 ttcgaccacg acccgctgat ccgcggacag gtgctcgagt gccttgccca gccagtcgcg
     3781 gagttgacga tcctcatccg ggtcgtgcga agggccgcgg tcttcccgca acgcgtaatc
     3841 gtccaccggt tcgtcgccgc gctgccgcag cgccttcagg ccgcagcggt aggcgatgcc
     3901 catgagccag gtggaaacgc gcgaatcgcc gtggaatccg tccgccttcc gccacgcgat
     3961 ccagaagcaa tcgttgatga cctcctcgat caggtcggcc cgccgcgtca gccgcgaaag
     4021 aaagcgcgcc aggccgccgt gataggcgcg atagagcgcc tccagcgccg cgcgatcccc
     4081 tgccgccatg cgctggagga gcaggcggtc tcgggaatcg gcctcgtctc gggcttcggt
     4141 catcgatgct gtggaacgac caatctcaag ggtgggtgcc ggtccggcgg gggaaggttg
     4201 ccttggcgaa ggcggattcg cggggcgggt gcgagcggga gggcttcaga aggaccggga
     4261 aatcgtcgcg agccacggcg aagcgacccg gccctcgaga tacccgtgtg cgccggaatc
     4321 gaccaccacg cgatcaagtt cgacctgcca ggcgccttgg ctccagatca ggcccgcctg
     4381 gccataacgg taataaccgg tgtgatagcg ctcatgggcc gaatgtccat agggcacggc
     4441 gtaacgggcg atgccgagcc cagccgaaag ggcaaaattc cggcccagcg gccaatggaa
     4501 attcaggtcg gcggccgggt gcaactcgtg cccctcggtc cccacgacat ggatgcccga
     4561 cacgccgaac gtcagcacgt cgcggtaaag ccagtacagg cccggctcgt agacatcggc
     4621 gtc
//
LOCUS       DASSNH010000137         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_5057, whole genome shotgun sequence.
ACCESSION   DASSNH010000137 DASSNH010000000
VERSION     DASSNH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5057"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFK00_04990"
     CDS             <1..304
                     /locus_tag="VFK00_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7592343.1"
                     /translation="ITASVDFTALAEAGIAAGFEPVACCFQSEFLVAAGLEELFAAAY
                     AAEPDEAARYALAQQVKRLTLPGEMGERFEAMLFARGIDADAAFAPLVAVDRSERL"
     gene            complement(391..1629)
                     /locus_tag="VFK00_04995"
     CDS             complement(391..1629)
                     /locus_tag="VFK00_04995"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HET7592344.1"
                     /translation="MADAGHRVFLVGGAVRDAMLGRPHGDRDYVVVGATPEDMLAQGY
                     KPVGKDFPVFLHPDTGEEYALARTERKTGPGYHGFAFHAAPDVTLEQDLARRDLTINA
                     MARDEAGALVDPYRGERDLRERILRHVSPAFAEDPVRILRVARFHARYAPLGFRIADE
                     TVALMRSMVEAGEAAHLVPERVWAETRKALAEPEPSAFLRALRTCGALAVVFPEIDAL
                     YGVPQPPQHHPEVDCGAHLELVLDQAARLAPGDDLAGFLALTHDLGKARTPAAELPKH
                     IGHEHRGLAPLRALCERLRVPAEYAELAALTCRHHLESHRALELKPATVLKLLEAFDA
                     FRRPARLDPFLIACTADKRGRLHHENDAYPQADFLRAARDAARAVTAQPFIEQGLQGT
                     AIGDAMRRARIDAIAALPRE"
     gene            complement(1633..2571)
                     /locus_tag="VFK00_05000"
     CDS             complement(1633..2571)
                     /locus_tag="VFK00_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET7592345.1"
                     /translation="MNPKRYLVLGGTGFIGRNLLAALAPSGCGITVLSRNRERRRAIN
                     VLPNVRTFSADVYDRKVLEKYLVGADAVINLVGILNESGNATFTHAHVDLTATLIAAC
                     RQAGVRRIHQMSSLNAGERASKYLKTRGEAEAQVRNSGMEWTIYRPSLVYGQGDGLVF
                     RFLKLLKLLPVLPLAQPHAKIAPAYVGDVAEAFVRCLGDRGTIGRIYELYGPDTLELV
                     RIVRMIRDAAGLRRPVIPLSAGLGRLQAAMAEMIPGKPFSRDNFHSLGVDSVGTRDGF
                     GELGISPRRFAAMLPSVLGEHARAYLADIRRATQEI"
     gene            complement(2568..>3718)
                     /locus_tag="VFK00_05005"
     CDS             complement(2568..>3718)
                     /locus_tag="VFK00_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7592346.1"
                     /translation="LPAEAQSDATREWRVRIAVAQGNWKAARAAINALSPDQLQRDEW
                     RDWRARVAQKLGQADTARDDYAALAQQATFYGFLAADRAGLPYTICPETIPADAIADA
                     KIKAIPGFARAFEFFALGMLHSARREWSRAFQDLSPPEQRQAAALASRMGWYDRAIFA
                     FGKSGDLQYYALRFPLADEDRVLEAAQAAGIDPAWTFAIIRAETAWQPDARSGADARG
                     LMQLLPGTAELVSRRSGLPYPGPSSLYDPDVNIPLGTRYLAHLGMRFNGSPWLTSAAY
                     NAGPGNAQRWVDARGNLDPEVFILTIPFNETRDYVTRVMSFATIYGWRLHGAPMAVSS
                     RLPAIGTPYDPAVDPPRKQVVCKTPAPAAPAASTATPATPAPARSASQ"
BASE COUNT          544 a         1241 c         1351 g          582 t
ORIGIN      
        1 catcaccgcc tcggtggatt tcaccgcgct cgccgaagcc gggatcgcgg ccggtttcga
       61 accggtggcc tgctgtttcc agtccgaatt cctggtcgcg gccgggctcg aggaactgtt
      121 cgccgcagcc tacgccgccg agcctgacga agccgcacgc tacgcgctgg cgcagcaggt
      181 gaagcgcctc accttgcccg gggaaatggg cgagcgcttc gaggcgatgc tgttcgcgcg
      241 tgggatcgat gccgatgccg cgttcgcccc cttggtcgcg gtcgatcgca gcgagcggct
      301 ttgacgcggg ccggccgccg gcctgccctc cgaccagcgg cggctcgcgc gcaagcggct
      361 accgcaggct gaagccccac tgcgatctcg tcattcccgc ggcagcgcgg caatggcatc
      421 gatgcgcgcg cggcgcatgg cgtcgccgat ggcggtgccc tgcaaaccct gttcgatgaa
      481 cggctgcgcg gtgaccgcgc gcgcggcgtc gcgggcggcg cgcaggaagt cggcctgggg
      541 gtaggcgtcg ttttcgtggt gcagccggcc gcgcttgtcg gcggtgcagg cgatgaggaa
      601 gggatcgagc cgcgcgggac gccggaacgc atcgaaggct tccagcagct tcaacaccgt
      661 cgccggcttc agttccagcg cgcgatgcga ttcgaggtgg tgccggcagg tcagcgctgc
      721 gagctcggcg tattcggcag gaacgcgcag ccgctcgcac agcgcgcgca gcggggcgag
      781 gccgcggtgc tcgtggccga tgtgtttcgg caattcggct gccggcgtcc gcgccttgcc
      841 gaggtcgtgg gtgagcgcca ggaatccggc gaggtcgtcg cccggcgcga gccgtgccgc
      901 ttgatcgagt accagctcga ggtgtgcgcc gcaatcgact tccgggtgat gttggggtgg
      961 ttgcggtacg ccgtacaagg cgtcgatttc cggaaacacg acggcgagcg cgccgcacgt
     1021 ccgcagcgcg cggaggaacg cggagggttc gggttcggcg agcgccttgc gggtttccgc
     1081 ccacacccgc tcgggcacca gatgcgcggc ctcgccggcc tcgaccatcg agcgcatcaa
     1141 ggcgacggtt tcgtcggcga tcctgaaacc gagcggcgca tagcgtgcgt ggaaacgcgc
     1201 gacccgcagg atccgcaccg gatcctcggc gaacgcgggc gacacgtgcc gcaggatccg
     1261 ttcgcgcaag tcgcgttcgc cgcggtacgg atcgaccagt gcaccggcct cgtcgcgcgc
     1321 catcgcattg atcgtgaggt cgcggcgggc gaggtcctgt tccagcgtca cgtccggcgc
     1381 ggcgtggaac gcaaagccgt ggtagccggg gcccgtcttg cgctcggtgc gggccagcgc
     1441 gtattcctcg ccggtgtcgg ggtgcaggaa caccgggaaa tccttgccga cgggcttgta
     1501 gccctgcgcc agcatgtctt cgggcgtcgc gccgaccacg acgtagtcgc gatcgccgtg
     1561 cgggcgtccg agcatggcgt cgcgcaccgc gccgccgacc aggaaaacgc gatggccggc
     1621 atcggccatt cgtcagattt cctgggtggc gcgtcggatg tcggccaggt acgcgcgcgc
     1681 gtgctcgccg agcacgctgg gcagcatcgc ggcaaaacgt cgcggcgaga tgccgagttc
     1741 gccgaagccg tcgcgcgtgc ccaccgaatc gacgccgagc gaatggaagt tgtcgcgcga
     1801 gaacggtttg ccggggatca tttcggccat cgcggcctgc agtctgccga gccccgcgga
     1861 cagggggatc accggccgcc gcaatccggc ggcatcgcgg atcatccgca cgatccgaac
     1921 cagctccagg gtgtcggggc cgtagagttc atagatccgc ccgatcgtgc cgcggtcgcc
     1981 gaggcagcgt acgaaggctt cggcgacgtc gcccacgtag gccggcgcga tcttcgcgtg
     2041 cggctgcgcc agcggcagca cgggcaagag cttcagcagc ttcaggaacc ggaacaccag
     2101 tccgtcgccc tgcccataca ccaggctggg ccggtagatg gtccactcca tgccggagtt
     2161 gcgcacctgg gcttcggctt cgccgcgcgt cttcaggtac ttcgaggcac gctcgccggc
     2221 gttcagcgag ctcatctggt ggatgcgccg cacccccgcc tgccggcaag ccgcgatcaa
     2281 ggtcgcggtg agatcgacgt gcgcatgggt gaaagtcgcg ttgccgctct cgttgaggat
     2341 gcccaccagg ttgatgaccg catcggcgcc cacgaggtac ttctcgagca ccttgcgatc
     2401 gtagacgtcc gcgctgaacg tgcgcacgtt cggcaatacg ttgatcgcgc gccgccgctc
     2461 gcgattgcgg ctgaggaccg tgatgccgca acccgacggc gccagtgccg cgagcaggtt
     2521 gcgaccgatg aaaccggtac cgccgagtac gaggtaacgc ttggggttca ttgcgatgca
     2581 ctccttgcag gggccggtgt tgcgggtgtc gcggtcgaag cggccggcgc cgcgggtgca
     2641 ggtgtcttgc aaacgacctg tttgcgcggc gggtcgaccg ccgggtcata cggggtgccg
     2701 atcgcaggca gccgcgaaga caccgccatc ggcgcgccgt gcagccgcca tccgtagatc
     2761 gtcgcgaacg acataacccg cgtgacatag tcgcgtgttt cgttgaacgg gatcgtgagg
     2821 atgaagactt ccgggtcgag gttgccgcgc gcatcgaccc agcgctgtgc gttgccggga
     2881 ccggcgttgt aggccgcgga ggtgagccag gggctgccgt tgaagcgcat cccgaggtgc
     2941 gcgagatagc gggtgcccag cgggatgttg acgtcggggt cgtacaggct ggatggtccc
     3001 gggtagggca ggccgctgcg gcgcgacacc agctcggcgg tgccgggcaa caactgcatc
     3061 aggccgcgcg cgtcggcgcc cgagcgcgcg tccggctgcc acgccgtttc ggcgcggatg
     3121 atggcgaacg tccaggccgg gtcgatgccg gcagcctgcg cggcttcgag tacgcgatcc
     3181 tcgtccgcca gcggaaaccg cagcgcgtag tactgcaggt cccccgactt gccgaacgca
     3241 aagatcgcgc ggtcgtacca gcccatccgc gaagccagcg ccgcggcctg gcgttgctcc
     3301 ggcggcgaca ggtcctggaa cgcgcggctc cattcacggc gggcggaatg cagcatgccg
     3361 agcgcgaaga actcgaacgc gcgcgcgaag ccggggatcg ccttgatctt cgcgtccgcg
     3421 atcgcatcgg cgggaatggt ttccgggcaa atcgtgtacg gcagccccgc gcggtcggcg
     3481 gcgaggaaac cgtagaaggt ggcctgttgc gcgagcgccg catagtcgtc gcgggcggtg
     3541 tcggcctgcc cgagcttctg ggccacgcgc gcccgccagt cccgccattc gtcgcgctgc
     3601 agctggtccg ggctcagcgc gttgatcgcg gcccgggccg ccttccagtt gccctgcgcg
     3661 accgcgatcc gcacacgcca ttcgcgggtc gcgtccgatt gcgcttccgc cggcagcg
//
LOCUS       DASSNH010000138         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1254612, whole genome shotgun sequence.
ACCESSION   DASSNH010000138 DASSNH010000000
VERSION     DASSNH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254612"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VFK00_05010"
     CDS             <1..150
                     /locus_tag="VFK00_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7592347.1"
                     /translation="GSDAVFNIGCAVGAVQTLDDGVWIAMNGRIWDPRHVRKNRAANR
                     FEGLW"
     gene            complement(230..1810)
                     /locus_tag="VFK00_05015"
     CDS             complement(230..1810)
                     /locus_tag="VFK00_05015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HET7592348.1"
                     /translation="MSSKFRLKSLVAGLGLTAATLACAAAPGASTVAAHATALHPGDT
                     VVGPVSLTRPLRVAISLKLRNEASLQAFIANPNHTNLTPAQFTAEYGPSKADANAVAA
                     WLKQHGFSNISISPNRVLVSATASAGSVQSAFHTNMVRVRTHDGRSAFANTSNASVPA
                     SLHGVSGVIGLQSVHVPHRVKGKPPRGGGGSIIGHDPVQFPAIYGGASLPTAAGVTVG
                     IISNGDLRQTIADLNTFTSQNGLATVTTQIVNTDGTGTDTSGTSEWDLDSQDIVGMAG
                     GQVGKIVFYDIPTLLNTSMVDDFNTVVSANAAKVINVSLGECETSARSDGSAAAADAI
                     FQQADAQGQTFSVSTGDAGSNECGGSPHGTTPSWPASSPYVVAVGGTTLNATTTAWQG
                     ETVWADTGGSASTFEPIPSWQVGVGQNAGSRYRGLPDIAYDADPNSGAIIVVNGGLQQ
                     WGGTSLSSPLFVGFWARMIAAKGTGIGFAAPKLYQLPASAFHDVTSGSNGGESAAPGW
                     DYTTGFGSIIMNQAASSL"
     gene            complement(2254..4104)
                     /locus_tag="VFK00_05020"
     CDS             complement(2254..4104)
                     /locus_tag="VFK00_05020"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HET7592349.1"
                     /translation="MAPNQRLRVTMSLHWRNQAQLQAFIAQPHHKNLSHAEFMAAYAP
                     TTAQVDQVKAFMQKQGFTHVSVTPDNLLVSGEASVGTVQSAFRTSMVNVRTRNGRLAF
                     ANATPARIPTALRGVVNEVLGLQNVHKFHVMPAKPMASTQATTGHNPADFASIYGAGG
                     TATASSVSVGIITQGSMSNVLDDFQTFLGQNGYGNIPMNVVTVDGGSSDTSGDAEWDM
                     DSQAIVGISGGVQALYLYDIPQLTTQDLVDGFNRATTSGQVRVVNVSIGGCESGAETN
                     GAATGDSVFQEADAEGITFSISSGDSGADECGDGGVTPSWPADSQYVVAVGGTTLNTS
                     GTSWTGETVWSGTGGSPSTFEAMPSWQQGVGQNAGHSTRGVPDVAFDGDPNSGALVIV
                     DGSTEQIGGTSLSSPLFVGVWARTLAADSSLGFAAPLVYADAASHYASDFHDVTQGGN
                     GGESAATGWDYTTGFGSINIASFVGNVAGSGGGGTSPPPPPPPPPPPPPGDTFSNNHQ
                     YVIWDDSTATSKISVTGEPGAAPSDLPVHVNITHNWSGDLEIVLYAPNGAYAILQYPD
                     YDNDGNIDKTYDINASSVSSNGTWKLKVIDDNIWGDGADYGTLNGWSMTF"
     gene            4112..>4315
                     /locus_tag="VFK00_05025"
     CDS             4112..>4315
                     /locus_tag="VFK00_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592350.1"
                     /translation="MTESPGRKRVACVTTAVLAAPAGGVAAMAADPAARPMATTKAFN
                     LGLLGIIELLLDGRSTDAQRDTHP"
BASE COUNT          705 a         1396 c         1437 g          777 t
ORIGIN      
        1 ggctcggatg ccgtgttcaa catcggctgc gcggtgggcg ccgtgcagac cctggacgat
       61 ggcgtgtgga tcgcgatgaa cggccgcatc tgggatccgc gccacgtccg caagaaccgt
      121 gccgccaacc gcttcgaggg actctggtaa caagccttcg cgatcccgtg gcgccagaaa
      181 aaagagcccg gcaatgcgcc gggctccagg tcctcgtgga aaccggtcgt cagagcgatg
      241 aagccgcctg gttcatgatg atgctgccga acccggtggt gtaatcccaa cccggcgccg
      301 cgctctcgcc accgttgctg ccggaagtca cgtcgtggaa ggccgatgcc ggcaactggt
      361 agagcttcgg cgcggcaaat ccgataccgg tgcccttggc ggcaatcatg cgcgcccaga
      421 atccgacgaa cagcggcgac gacaggctgg taccgcccca ctgctgcagc ccgccgttga
      481 cgacgatgat cgcgccacta ttgggatcgg catcgtacgc gatgtcgggc aggccacgat
      541 accggctgcc ggcgttctgg ccgactccga cctgccagct cgggatcggt tcgaaggtgc
      601 tggcgctgcc tcccgtatcg gcccacacgg tctcgccttg ccaggccgtg gtggtggcat
      661 tgagggttgt gccgcccacc gcgaccacgt agggcgagct ggcgggccag ctcggcgtgg
      721 tgccgtgcgg gctgccgccg cattcgttcg agcccgcgtc tcccgtggat accgagaacg
      781 tctgcccctg ggcatcggcc tgctggaaaa tcgcatcggc cgctgcggcc gaaccatcgc
      841 tcctggccga ggtttcgcat tcgcccagtg atacgttgat cactttggcc gcattcgcgc
      901 tgaccacggt attgaagtcg tccaccatac tcgtattcaa cagagtcggg atgtcgtaga
      961 agacgatctt gcccacctgg ccgccggcca tgcccacgat gtcctggctg tccagatccc
     1021 attcggaggt gccgctggtg tcggtcccgg tgccgtcggt gttgacgatc tgtgtcgtca
     1081 ccgttgccag accgttttgg gaggtgaagg tgttgaggtc ggcgatcgtc tggcgaaggt
     1141 ccccgttgga aatgatcccg accgtgacgc cggcggcggt cggcagactg gcgccgccgt
     1201 aaatcgccgg gaactgaacg ggatcgtggc cgatgatcga gccgccgccc ccgcgcggcg
     1261 gtttgccctt cacgcgatgc ggcacatgca cgctctgcag gccgatcacg ccgctcacgc
     1321 catggagcga cgcgggaacc gaagcattgc tcgtgttggc gaacgcgctg cggccgtcat
     1381 gcgtgcggac ccgcaccatg ttcgtgtgaa aagcggattg cacgctaccg gcggaagccg
     1441 tcgccgaaac caacacgcga ttgggcgaaa tcgagatgtt ggaaaagccg tgttgcttca
     1501 gccatgcggc gacggcattc gcatctgctt tgctcggccc gtattccgcc gtgaactgcg
     1561 ccggagtgag attggtgtgg ttcggattgg cgatgaacgc ctgcaacgac gcctcgttgc
     1621 gcagtttcag cgaaatcgcc acgcgcagcg gccgggtcag cgagaccgga cccacgacgg
     1681 tgtcgcccgg gtgcagcgcg gttgcgtgcg cagctaccgt gctggcgccg ggcgccgcag
     1741 cgcaagccag cgtggcagcg gtgaggccaa gcccggcaac caggctcttc aaacggaact
     1801 tgcttgacat cgagatctcc ttgcggggta cacgaaagtc gaccgggctg cgaatctgcg
     1861 cgtggagcgc aggacgaact gccggccggg ttgatccctc cccgagatac actgcccgga
     1921 cttcgcacgg acacgcacga agcgcgcggg tgatgcgtgc cttgagacga ccgcctgcga
     1981 cgccctcgcg atcacaggct tcagcacgct agtcgcattt gcgaatacat gtcacgctgc
     2041 aacgcagcaa ggtgcgcgtt cggcccggaa cggtgctgca gggtgccctc gcgccgtgac
     2101 ccgttgtctt cgccggggca aggcgcacgc tttctgccag cgggtatcgc ggggggggcc
     2161 ggatgtgcga cagccatggt ggccgaaaaa gggtccggcc cgaggaccgg acccaagcat
     2221 tgcaatgaag cggttgcctg acggccgttt tcctcagaac gtcatgctcc agccgttcaa
     2281 ggttccgtag tcggcaccat caccccagat gttgtcgtcg atcaccttga gcttccaggt
     2341 gccgttgctc gatacggacg aggcgttgat gtcgtacgtc ttgtcgatgt tgccgtcgtt
     2401 gtcgtaatcg ggatattgca ggatcgcgta ggcgccgttg ggcgcataca acacgatttc
     2461 cagatcgccc gaccagttgt gcgtgatgtt cacgtgcacc ggaaggtcgg agggtgcggc
     2521 acccggctcg cccgtcaccg agatcttgct ggtcgccgtg ctgtcgtccc agatcacgta
     2581 ttggtgattg ttcgagaacg tgtcgcccgg cggtggcggc ggcggcggag gcggaggggg
     2641 aggcggcgac gtaccaccgc caccactgcc ggccacgttg cccacgaagc tcgcgatgtt
     2701 gatcgagccg aagccggtgg tgtagtccca ccccgtcgcg gccgactcgc cgccgttgcc
     2761 gccttgtgtc acgtcgtgga aatcggacgc gtaatgcgag gcggcatccg cgtaaaccag
     2821 cggcgccgcg aagccgaggc tcgaatcagc cgccagggtg cgcgcccaca cgcccacgaa
     2881 cagcggtgag gagaggctgg ttccgccgat ctgttcggtc gaaccatcga cgatcacgag
     2941 cgccccggaa ttgggatcgc catcgaaggc cacgtcgggc acgccgcgcg tggaatgacc
     3001 cgcgttctgt cccacgccct gctgccaact cggcatcgcc tcgaaggtgc tggggctgcc
     3061 gccggtaccg ctccagacgg tttcgccggt ccagctcgtt ccgctggtat tgagcgtggt
     3121 accccccacc gcgaccacgt attgcgaatc ggccggccag ctcggggtca cgccgccgtc
     3181 gccgcactcg tcggcgccgg aatctcccga cgagatcgaa aacgtgatgc cttcggcgtc
     3241 ggcttcctgg aagacactgt cgcccgtggc cgcaccgttc gtttcggcgc cgctttcgca
     3301 accgccgatc gagacgttca cgacccggac ctggccactg gtggtggcgc gattgaagcc
     3361 gtcgaccaga tcctgggtgg tcaactgcgg gatgtcgtac aggtacagtg cctgcacgcc
     3421 gccgctgatg ccgacgatcg cctggctgtc catgtcccat tcggcgtcgc cgctggtgtc
     3481 ggaactgccg ccatccaccg tcaccacgtt catcgggatg ttgccgtagc cgttctggcc
     3541 gaggaacgtc tggaaatcgt ccagcacgtt gctcatgctg ccctgcgtga tgatgccgac
     3601 gctgaccgac gacgccgtcg cggtgccgcc ggcgccgtag atcgacgcga aatcggcggg
     3661 gttgtgcccg gtcgtcgcct gggtcgacgc catcggcttg gccggcatca cgtggaactt
     3721 gtgcacgttc tgcaagccca gcacttcgtt gacgacaccg cgcaatgcgg tgggaatgcg
     3781 cgccggcgtg gcgttggcga acgccagccg cccgttgcgg gtgcgcacgt tcaccatgct
     3841 ggtgcggaac gccgattgga ccgtcccgac cgacgcttcg ccggacacca gcaggttgtc
     3901 gggcgtcacc gagacatggg tgaaaccctg tttttgcatg aacgccttga cctggtccac
     3961 ctgcgccgtg gttggcgcgt acgcggccat gaactcggcg tggctgaggt tcttgtggtg
     4021 cggctgggcg atgaacgctt gcaattgcgc ctggttgcgc cagtgcagcg acatcgtcac
     4081 gcgcagccgc tggttgggcg ccatcgcacc gatgacggaa tcgcccggtc gcaagcgggt
     4141 cgcctgcgtg acgaccgccg tattggccgc acccgcgggc ggcgtcgcag caatggccgc
     4201 cgatccggcg gccaggccca tggcaacgac taaagccttc aatctgggat tgcttggcat
     4261 cattgagctc ctgctggatg gaaggagcac ggatgcgcaa cgcgacacgc atccg
//
LOCUS       DASSNH010000139        34573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1005892, whole genome shotgun sequence.
ACCESSION   DASSNH010000139 DASSNH010000000
VERSION     DASSNH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 34573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34573
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005892"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1198
                     /locus_tag="VFK00_05030"
     CDS             65..1198
                     /locus_tag="VFK00_05030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET7592351.1"
                     /translation="MSHAAARTPQRAARAYQDRILPAVSRTFALTIPQLDPRLRTVIA
                     NAYLLCRIADTIEDEQSLAADVKQQFHDRFTRVVASTEDPASFARDLSPLLNGNTLAA
                     ERDLVEHAPAIIEITDSFNLRQREALTRCIRTMCTGMPSFEREASTAGLADMREMDRY
                     CYYVAGIVGQTLTELFCDYSPRIAEHREAMSSLDVSFGQGLQMTNILKDFWEDRDRGV
                     CWLPRDLFAEEGVDLKSIRRNDVPAAFGHAYAQLIGVAHSHLRNALNYTLLIPEKEVG
                     IRRFCLIAVGLAMRTLASILAKPDFTAGAQVKVSRQVVKKVVIATHLFAGYSRVLRRW
                     FARLAYGLPLQALAEDWGGLLPGPHSAWPRSDACGPAHAVTSK"
     gene            1201..3147
                     /gene="shc"
                     /locus_tag="VFK00_05035"
     CDS             1201..3147
                     /gene="shc"
                     /locus_tag="VFK00_05035"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET7592352.1"
                     /translation="MAPDSGIPHGRPAAHAGITTRERTPGPLAAAIDSARSSLLAHQH
                     AEGYWQYELEADCTIPAEYILMLHHLGESDRLLESRLAAYLRDHQHRDGGWPLYPGGA
                     MDVSCSVKCYFALKLAGDRPDAPHMVRARSAILERGGAARANVFTRIALALFGELPWR
                     GVPFVPVEIVLLPKWFPFNLSKVSYWSRTVMVPLSVLTSLKARAANPRGVHIRELFVT
                     PPEQERHYFQARSFLNRLLFACEQTLRRGERLIPRRIRKRALKRAEAWILERRNGDGG
                     LGAIFPAMVNAYEALALLGYPADDPARKETRKAIDDLLVVRDHEAWCQPCVSPVWDTG
                     LACLALQEDKHAPGEPVRRALDWLAERQLCDAPGDWREYRPRLPGGGWAFQFRNDAYP
                     DLDDTAVVARAMHQSPDRARHAEAIARAADWLVGMQSRNGGFAAFDAENDHGWLNQIP
                     FADHGALLDPPTSDVSGRVLTLLSMLDRPGDRESRDRVLRFLLDEQTPDGAWFGRWGT
                     NYVYGTWSVLVALRAAGMPVDAPRIRRAARWLKSVQQADGGWGEGNDSYLDPRLRGRG
                     ARSGPTQTAWALLALMQAGEADSPAVARGIDYLLKTQVDGEWRDTRFNAPGFPRVFYL
                     KYHGYSRYFPYWALVRYRNVRGKG"
     gene            3134..3856
                     /locus_tag="VFK00_05040"
     CDS             3134..3856
                     /locus_tag="VFK00_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592353.1"
                     /translation="MARAECVGLVVALPAEARSLGAPRLHPGDCAPWQHGWVAVSGIG
                     PHNAMRAAERLLACGVSQLANWGVAGALDDSLAPGDIVVPECIRHAPDDPGFATDAVT
                     CDRIVAACSATLPVRRGALWSSDTPITNPADKRALAERSGAIAVDMEAAGVAAVAARA
                     GLPFAAVKAICDPLGRELPRRIMRALDGGSGGPSWRMLSAIAFGGPPVWSAARGLAQD
                     FSQARRALTSAARLAARAAVAA"
     gene            3853..4845
                     /gene="hpnA"
                     /locus_tag="VFK00_05045"
     CDS             3853..4845
                     /gene="hpnA"
                     /locus_tag="VFK00_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HET7592354.1"
                     /translation="MSARVLVSGANGFVGSAVCLALLRHGFTVRALARPTSDIASLDG
                     LDVEIARGDLLEPPTLRQALQGCEGLFHVAADYRLWARNPEEIRMNNVRGTRNILAAA
                     RSTGVARVVHTSSVATLGLHEDGTPSDEETPASYADMIGEYKRSKYLSEKMARRRARA
                     GQDIVIVNPSAPVGPRDRKPTPTGQMIVDAARGKMPAYVDTGLNIVHVDDVAEGHVLA
                     FQRGERGRRYILGGENMSLREILSTVAELTGRAGPRVRLPHRALLPVARAAEAWARVR
                     DRAPTITVDGVRLAQHKMYFTSRRAETELGYRARPAREALSDAIQWFRANGYCR"
     gene            4842..5873
                     /gene="mvaD"
                     /locus_tag="VFK00_05050"
     CDS             4842..5873
                     /gene="mvaD"
                     /locus_tag="VFK00_05050"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET7592355.1"
                     /translation="MTSPPGRATALAHPNIALVKYWGKRDDTLNLPASGSLSITLDGL
                     HTRTSVRFDPALGEDDIELNGRCDEAESRKISAFLDLLRTRAGIATRARVASANDFPT
                     GAGLASSASGFAALVVAADRALGMGLDASSLSRLARRGSGSAARSIFGGFVEMAAGAR
                     ADGEDAFATPLLDPEAWPLSVVIAITTRAAKATSSRVGMDLTRRTSPFYRDWIAGGAR
                     DLDAARAAVRVRDFDKLADVGEASCLAMHAVMLSTRPGLVYWNGATVECMRRVRALRD
                     TEGLGTFFTIDAGPQVKAVCLPEAAGRVADALREIPGVEDVLVSGLGHGARVVEERAV
                     EDCAAGDDG"
     gene            5873..6895
                     /locus_tag="VFK00_05055"
     CDS             5873..6895
                     /locus_tag="VFK00_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592356.1"
                     /translation="MHVTASAPGKLVLLGEYAVLEGAPALVLAVDRRARVALTPTAGP
                     AWEIVSPTLGAEASLRLGIDGVEWHHAPVPGLEWLAALFAQLPFAATLPPCRVELDSD
                     AFFLDHADGRMKLGLGSSAAITVALIGALHACAGRPDPTLEEAIAVHRAIQHGRGSGI
                     DIAAALAGGLSRFELRRGAPGYVPMRLPHGLHWCCVFSGRPASTADMLARIAAWRERE
                     PAEYARRIRELATMALRGVDAVAGRDAASFLSSFEEYAHALARFGEAGGVDIASRGHR
                     VLAALAADCRVVYKSCGAGGGDVGVTFAMDDMRLQEFAGRATEAGFAVIELDADPRGL
                     ETNVAD"
     gene            6913..9171
                     /locus_tag="VFK00_05060"
     CDS             6913..9171
                     /locus_tag="VFK00_05060"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET7592357.1"
                     /translation="MDQSRMPGFYKLSVPERVNLVRERGMLSREDFQTLLSGGHTLTV
                     ARADKMIENVIGVMGLPLGLGLNFLVNGKDRVVPLVVEEPSIVAALSSAAKVVRAAGG
                     FTTRSTEPILIGQVQIVDVDNSPAAQNALLQRKQEILNLANSLHPKMVARGGGARDIE
                     VRIHPAADGRRDMVVLHLLVNTCDAMGANMVNTMCEGVAPLVEGITGGKVFLRILSNL
                     TDRALVWAKAVIPTKLLAGNGYDGEQVRDGIILANDLAVCDPYRAATHNKGIMNGVDA
                     VALATGNDWRALEAAAHAYAARGEHYTSLTRWYKDDEGNLVGNIEMPMKVGTVGGNLQ
                     SNATVALNLRLLKAQGARELAEIMGAVGLAQNFSALRALVTDGIQQGHMTLHARSVAA
                     TAGAAPEIFDTVVERLLDSGEIKVWKAKEIIAEVTDQAQPEAKTVAHGFEPVDEAEYA
                     TGYGKAILLGEHAVVYGRHAIAVPVPMAIRARAEESKDGTQLIIPRWGVEQRLVPGTK
                     HPGSLLQSLDLILQKLGLTKRPLRIQVYPSLPRANGLGGSAALAVAVIRALDRHCALG
                     LTDSEVCALSYECERVAHGTPSGIDNTVATYGQPLLFQRGEPPVVEPLHFAKPLPMVV
                     GLSGVESLTAKTVRCVREAWQRNRKLYERIFDEIDALAMQGIKALAAWDLQQFGELMN
                     ICQGQLNALQVSSWELEEMIQVARANGAVGAKLTGGGGGGAMIALCPDNPGRVVRALQ
                     QAGYQAMETNIG"
     gene            9164..9778
                     /gene="idi"
                     /locus_tag="VFK00_05065"
     CDS             9164..9778
                     /gene="idi"
                     /locus_tag="VFK00_05065"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET7592358.1"
                     /translation="MADDADNRVVSFESERLILVDSADREIGHASKADAHAGQGILHR
                     AFSLFVFNPAGELLLQQRATGKPLWPGYWANSCCSHPRGGEDMETATRRRLAEELGFT
                     CPLEFVYKFQYHAEYGEAGSESELCWVYVGTSDAPVRINETEIAAWRYVAPDALDREI
                     AEAPDRFTPWLKMEWQRLRGEFAERLPGARVAQPPSRTRSFATD"
     gene            complement(9820..10902)
                     /locus_tag="VFK00_05070"
     CDS             complement(9820..10902)
                     /locus_tag="VFK00_05070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET7592359.1"
                     /translation="MSSDGEAAVGLHLDAAGAAVGDAAPATVRARTNNFDAIRLVAAL
                     LVLCSHQLFFLGKAQPTVGGYTLGELAVMMFFVISGYLVAESWYRDPHLVRFILRRFL
                     RLWPGLAAATIVIAAASVFLTSMAAGDYLGAVRHFVARNLQLRIVYELPGVFAGGSGA
                     AMAAVNGSWWTIPVEAKCYLYLALLGVVGLRRRWLSVLAFAVVAFLYLQSPTGQGLKY
                     LYILFFATGLCARQFTAEVLRHRIAWSCAGLAAFLAAVASGRQDLATWVIVAPLVLLT
                     GSMDTPGIRSAGRFGDLSYGIYIYAYFVQQLSVRYWPANGSLAGTLVVSVVATAALAW
                     CSWHAVEAPALALKRRLRRWFPDRVA"
     gene            11184..12314
                     /gene="hpnH"
                     /locus_tag="VFK00_05075"
     CDS             11184..12314
                     /gene="hpnH"
                     /locus_tag="VFK00_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET7592360.1"
                     /translation="MSIPVRQQLKVASYIIGQKLRGAKRYPLTLMLEPLFQCNLACPG
                     CGKIDYDSHILKQRLSVEECMQAIDECGAPMVSLAGGEPLVHKDIKEIVDGFLARGKY
                     IYLCTNAILLEKKLDLFTPSDHLTFSVHLDGMRERHDESVGREGVFDTAVAAIREAKH
                     RGFRVNINTTLFTTAKPDEVAEFFDFATYDLGVDGITFSPGFAYEHAPRQDVFMGRRQ
                     SKELMRKIFRRRKETRSKWALSQSSLFLDFVCGNQGYQCTAWSMPCRNVFGWQKPCYL
                     LVDEGYVPTFKDLVEKTDWDNYGVGRNPKCANCMAHCGFEGTAADDSFRHPLKAFWAS
                     VRGPKLSGPYAADPPILYDTPEPNQRSPQSNVPIAHFGGIRN"
     gene            12343..13452
                     /locus_tag="VFK00_05080"
     CDS             12343..13452
                     /locus_tag="VFK00_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7592361.1"
                     /translation="MLILAAASFALWLAVLLVPWQPWRCKERLEADAADDAQASGFTV
                     LMPARNEAAVLEETLAALRACAPAAPVVIVDDQSSDATADIARGSGLGNLRLVPGTPP
                     PAGWAGKLWALEQGLQHVSTPRVLLLDADIRLAPGMPAALQKKANEGYALVSVCAQPC
                     WTGAAARWLLPAFVYFFKLLYPFRLANRSDSRVAAAAGGVVLVDRRALADAGGFGAWH
                     DAIIDDCTLAAHVKRAGHHCWIGLTRGATSLRRSDAGDIAHMVARSAFVQLRESVILL
                     LVATALLVLAFWIPVLAVATGDGAARWLGLAAWLVLIACYLPTLAYYRRNGLTAVLLP
                     ATATFFLAATWFSAWRALAGTRSVWKDRRYQRGTG"
     gene            13453..14754
                     /locus_tag="VFK00_05085"
     CDS             13453..14754
                     /locus_tag="VFK00_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592362.1"
                     /translation="MQNRAEPASSVRMPQAGAIPDAGPHPLASWLLQFAADLYITAVY
                     HREVILPPGFRMRPGSIIASNHQRDVDGPVLGTALLDRHGLRFAGVLPYFVTREDLFR
                     PGILARLAVHWPRAFSGLLRRISLAWFFPLGRTEPMRRTREFTLGDALRALVAEGLGD
                     ADAGSLLNARGQRETGARPGELTVDALLERDDDALEQWWGLRRFSRPARATIAPGFRA
                     TVERQIAHFAARLDQGCCIYFAPEGTISTHGPFGRIRGGFYRIACATESPPWIQPIGL
                     GYDTLAPGKSRVVIRIGEAFRADTRMRRCEFDAKLRRAVLDLVTITPSHLLARFLLHG
                     PASFSVEELSRWLAQVHANLSANNAAVDPLFERVRFERIAARRLRWLHRKGLVARVGD
                     RFRNTCPRDLQPGWRRPGNVARYLDNALSDFLPDIERLAPC"
     gene            14748..15755
                     /locus_tag="VFK00_05090"
     CDS             14748..15755
                     /locus_tag="VFK00_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET7592363.1"
                     /translation="MLKNLWSMLRHVLKPQVVLPVLLAAALLVFAFNLGDLGKVVSRL
                     RDLPLHVVGISLGCATIYLGCKAAQLRLMLTRIDVCIPPRSFWLAFAVGELTVTLPLG
                     LFSQNWVMSAARRVHVGRSAAATVMMLLVEIAVVFLFLAVIGVPGWGEVRWVAVGLLV
                     LFVAFVAAAVVFEDKARKLASGVRREWLKKVAKGGVELLDGLRKLCTPHTIGVSLVLA
                     AVYLGALTAAFWQVGRGMHVQHLDYIDASTIYMFSLAVILIGAGLVSQIGTVDLLGMV
                     IARAFGIGYTDGLAMMLGFRIVWTGSIWVLCLPIVGATWREMPSRGKRKSGDGGKQES
                     G"
     gene            complement(15768..17675)
                     /locus_tag="VFK00_05095"
     CDS             complement(15768..17675)
                     /locus_tag="VFK00_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7592364.1"
                     /translation="MKANEDQPGPLVTRRRARRGAFRLALVVSVCSVGLHVAPGRASE
                     PLDCNPMYPQINIHDDRSVAPGTFCALYTVDIHSPGSLTNAGTVFNFQRVVNHDGGIL
                     LNAATGVFWNTVKTQLINDGSVQNFGTLTNDGASWGHAEIINGGAFSNYGTLTNGNYG
                     VLTNSGSFVNQGGDIRNFGQMASYGTIVNQLHGVLANFGTLQSSTLQNLVGAQLINQG
                     TLVSFGSFNNMQATVQNSGHLTNASTLTNLMGTITNVGDLDNLGTLRNRFEGTLTNVG
                     SLSNSGSLRNEAGARLTNFGSLSNLVSLRNESGATLTNFTSLSNVGTLRNEAGATLTN
                     HGTLKVKAGTLTNDGTLTNAPSGTVTNHGRFDNRASGYFDNAGTLRNDGEFVNAGTLK
                     STGEIRGSGVFAQTAGTTTLQGDLSQGLIRIDGGTLAGSGHVTAASLLHLGDGAVLSP
                     GEEPGDIGSFIIDAQSIELYGSMHLDVDSLASFDTLVSSSTVMFDPFAIGLGSTWFDF
                     YLGNNTSQRDGDTFDFFSASAFFNLDWVNFRCHGLMQGFGCELNEINDGRGLQLALSG
                     PSGGGGDGGGGVSVGVPEPGAFGLMCLGLALLGGGLGWSRWRDAVPMCIRRASDPKTV
                     ARSRRRRSRHG"
     gene            complement(17951..19030)
                     /locus_tag="VFK00_05100"
     CDS             complement(17951..19030)
                     /locus_tag="VFK00_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7592365.1"
                     /translation="MPRKVLFGVYHWGLGHATRSLGLIRALLDRGDEVTILMQPGGGM
                     SLLKSELGDACEYLPFQDTPAPFSRYPVVFYVRMTAAMPWILAGFRREHRFTEKLVAE
                     RHFDRVVSDSRFGVWSREVPSYCILHSIHQRVPFFSRFTEWCAERGQRDLLKGFTRIL
                     IPDVEENGGLSGWLGHEPSFDWGEGRLAYIGPLSSVPRMDVEQDIDVFFSVSGIEPQR
                     GLLEELVLDSLPRLSGRIVVTLGRPGHAGECREIAGATVYDYLDRKRQAEMLNRAKLV
                     MTRSGYTTLMELAGLGRRALFTATPGQSEQEYLARFHRERGHVWSVEQKHLDIPRDLE
                     RARAAAGIPRMDTTASVGNFLAAIG"
     gene            19255..19331
                     /locus_tag="VFK00_05105"
     tRNA            19255..19331
                     /locus_tag="VFK00_05105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19289..19291,aa:Arg,seq:cct)
     gene            19479..21011
                     /locus_tag="VFK00_05110"
     CDS             19479..21011
                     /locus_tag="VFK00_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7592366.1"
                     /translation="MSHAILSALGLADTNSGTFLGNGEWSKTTDAGVLEPVNPATGEP
                     LAKVYATSDADYETVIANARKAFEEWRKTPAPRRGEAIRLCAQALREHKDALGSLVAL
                     EMGKIKPEGDGEVQEMIDIADFAVGQSRMLYGNAMHSERPGHRMYEQWHPLGLIGIIS
                     AFNFPVAVWSWNAFIAAACGDVCIWKPSPKTPLSAIASVRICNEALKAGGFPEIFYLI
                     NDAGNELAQKFVDDKRIALISFTGSTKVGRHVGERVAARMGRSLLELGGNNAIILDRS
                     ADLKLAIPAIVFGAVGTAGQRCTSTRRLFVHESIIDDVLDKLRHAYQQVEKKIGDPTD
                     PATLMGPLNSKAAVEQFANAVKTAKAGGARVVSGGSVLERKGNFVEPTIITGVKNSDE
                     IVQTETFAPILYVMPFKTLDEAIEMQNDVPQGLSSAIFTQNLKATEQYLSAMGSDCGI
                     ANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWSDALPLAQGIKF
                     DI"
     gene            21177..21938
                     /locus_tag="VFK00_05115"
     CDS             21177..21938
                     /locus_tag="VFK00_05115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7592367.1"
                     /translation="MHNPFDFAGYRIAIAGGSRGIGRAMALGFLEAGAQVAVCARGQE
                     GLDALCSDAGEYAKKLHTHTCDLADADQIEHWIGDAARVMGGMDVLVNNATGYGFRDD
                     DESWLVDFNVDLMAAVRASRHALPHLRRSPHPCILHTSSIGSLRPRTMGPPYAAMKAA
                     LNHYAATQALALAGDRIRVNAIAPGSIEFEGGVWDQVKHAKPGQYAQVLATIPFGRYG
                     TAEEVAHLALFLASPLASWITGQVMVVDGGQSLRS"
     gene            22003..22287
                     /locus_tag="VFK00_05120"
     CDS             22003..22287
                     /locus_tag="VFK00_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET7592368.1"
                     /translation="MNTVYPRTSSLAVVSLIFGILAYVVLPGVGAVVAVICGHSARSE
                     IRRSPPGSIEGDGMALAGLVLGWIQIACAVIAVGFAILIIAGALAFHGFH"
     gene            22291..23331
                     /locus_tag="VFK00_05125"
     CDS             22291..23331
                     /locus_tag="VFK00_05125"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET7592369.1"
                     /translation="MYGALRPLLFALEAEAAHGLTLYALGVAQRSNLMRFVAHPPPDD
                     LPVRAFGIDFPNPVGLAAGLDKNGEHLDALGALGFGFIEVGTVTPRPQPGNPRPRLFR
                     LPGREALINRMGFNNDGVDVLVRNVEQTRWRGVLGINIGRNKDTPNERAADDYLACLE
                     RVYPLASYVTVNVSSPNTPGLRDLQEGDSLQRLIAVLVESREQLATRHGARKPILLKI
                     APDLDDAGMDAITAAVAGSGIDGLICTNTTIDRTGVAGEPHADEAGGLSGKPLFEPST
                     DVLRGMARRLGANMPLVGVGGISSGSDAADKLAAGAALVQVYSGLIFRGPWLLTECVE
                     EIRRQVGSSHVE"
     gene            23321..24376
                     /gene="murB"
                     /locus_tag="VFK00_05130"
     CDS             23321..24376
                     /gene="murB"
                     /locus_tag="VFK00_05130"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7592370.1"
                     /translation="MSNDNPAMDAAYTLTGSASLLQRNTLRVDARAAWLAEIHEADAI
                     PALFTEPVLRGKPVLLLGEGSNVLFAGDYDGAVVTMKARGVEVVSEDADAARIRVAAG
                     EHWDDLVRWTLAQGYPGLENLILIPGSVGAAPMQNIGAYGVEVGEFIESVEAFDLGGQ
                     RAVRFDTGQCEFGYRDSVFKRHPDRWLITAVTLRLPRTHAPRIDYAGLRQELERIGVA
                     NPAAIHVADAVVNLRTRKLPDPAVIGNAGSFFKNPIVPAPQAAALRSDHPQLPCWPGD
                     GGVKLSAAWLIDSCGFKGLRNGDAGVSSRHALVLVNHGRASGAELWALARHVRDGVRE
                     RFGVNLEPEPRIIGAPA"
     gene            complement(24484..24792)
                     /locus_tag="VFK00_05135"
     CDS             complement(24484..24792)
                     /locus_tag="VFK00_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592371.1"
                     /translation="MVVALYVVGALLLLVAAIALLVGGVLVVFLPQLIIGGVFLIVAL
                     AIERWRYKPVGSGRPDPAWTDTGERFVDPETGLMTAVYFDPAKGERHYMAVPGAEHGS
                     "
     gene            complement(24801..25121)
                     /locus_tag="VFK00_05140"
     CDS             complement(24801..25121)
                     /locus_tag="VFK00_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET7592372.1"
                     /translation="MIIVTTENVAGHRIAETKGQVFGLVVRSRGIGGNVMAGLRSIVG
                     GEISEYTQMLEEARRHATDRMVQNAQAMGANAVVMMRFDSSEIGQTMSEIVAYGTAVV
                     LEKV"
     gene            complement(25324..26082)
                     /locus_tag="VFK00_05145"
     CDS             complement(25324..26082)
                     /locus_tag="VFK00_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET7592373.1"
                     /translation="MRSRNPVLRDKFFQNLPATAEPMTLNGTLVRTGLLLVLMLITGA
                     WTWNHFMNASAGGDLAAGAAAVTPFLMGGLIVGLVLAVASTFKPNWTPVTAPLYALAE
                     GTALGGISALFEVRYQGIVLQAVMASIGVLAVMLFLYRSGVIKVTHKFRMGVAAATGG
                     ILLLYLVQWGISAFTGFGGMSFVFGGGTFGILFSLFVVGIAALNLVLDFDLIERATAA
                     GAPRYMEWFCAFALMVTLVWLYLELLRLLGNTRR"
     gene            complement(26266..27516)
                     /locus_tag="VFK00_05150"
     CDS             complement(26266..27516)
                     /locus_tag="VFK00_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7592374.1"
                     /translation="MDLNPASGSGAVEPVAPVRAARTPHGPTRYSLLAAVSFVHVLND
                     MMQSVIVAIYPLLKGEFDLGFFQIGAITLTFQLTASLLQPVVGMVTDRKPLPYSLPIG
                     MCFTLGGLLLLSVAPSYAVLLVAVALIGCGSSVFHPESSRVARMASGGRFGFAQSVFQ
                     IGGNIGQALGPLLAAAIVLKLGRSHVGWFGLAALLAIVILLQVSRWYSRHAEERVRKR
                     QPDEKPLFPAPVVRRTIGVLLILMFSKFFYLASISSYYEFYLIHRFGLSAHAAANLLG
                     VFLFAVAAGTLIGGPLGDRIGRRRVIWFSILGCAPFTLLLPHVGLGWTVGLSVVIGLI
                     LASAFPAIIVYAQDMLTHRIGMVSGLFYGFSFGLGGIGAAVLGVVADHFGIEFVYQVC
                     AFLPLLGLLATFLPDVRPPESDAG"
     gene            complement(27601..29895)
                     /locus_tag="VFK00_05155"
     CDS             complement(27601..29895)
                     /locus_tag="VFK00_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7592375.1"
                     /translation="MNAIKTPVPARHKGFNRAEIVDQNFSEYVHAWHGRALPAPRDDA
                     PVLPGSTCTARDFRELFESQLISRHLDLMARVLRVQNKVFYTIGSSGHEGNAMVARLT
                     RHTDPAFLHYRSGAFMAERSRKVPDVDPVVDSALSFAASSEDPASGGRHKVWGSKALW
                     VLPQTSTIASHLPKAFGTAVAIEQAPRMGHELPVPKDSIVICSFGDASSNHATAQVAF
                     NAAAYTAYQRLPAPVLFVCEDNGIGISVKTPSGWVANNFRDRKDLDYFFADGLDLAEG
                     HAEVQRAVEHCRGTRRPTFLHLRTTRLAGHAGTDFEIEWRSFEELMALEATAPLLRSA
                     SIALESGLYTQQALLDEYEAIRKRCFAAAEAAYGRPKLESLEAVVRPLAPYTPDAVAA
                     EAARADYSDRRAAIFGGKDKLPEVQPPRHLALQINAALHDLLAKYRESLVFGEDVAQK
                     GGVYTLTRGLYKAFKGARVFNTLLDETTILGLAQGYANMGMLPIPEIQYLAYFHNACD
                     QIRGEAASLQFFSNDQYRNPLLVRIASLGYQKGFGGHFHNDNSIAALRDIPGIVVGCP
                     SRGDDAATMLRTLAALCKVDGRVCVFLEPIALYMTKDLHESGDGQWQFEYPAPGQAMP
                     LGGGRVYNEGAEDLVVFTYGNGVPMSLRAAREIEKKRKWNVRIVDLRWLVPLNEAFIV
                     EQATAAKRVLVVDEGRRSAGVGEGIITALVEAGLGDKPIARVVGADTYTPLAGAALLV
                     LPGEADIVEAAGTLARGNGRRKRS"
     gene            complement(29892..30452)
                     /locus_tag="VFK00_05160"
     CDS             complement(29892..30452)
                     /locus_tag="VFK00_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592376.1"
                     /translation="MPPLAPTLETARLILRVPCREDFEAWAALGGDEQATRHIGGVQT
                     RFESWNRLLSAIGSWHVLGFGAFSVMRKADRQWVGRVGPLRPEGWPGTEIGWTLAREA
                     WGNGYATEAAIAATDWAFAHLGWSEIIHCIAPDNLPSQAVARRLGSVKRGQGTLPAPY
                     GADPIDIWGQTREQWLARRTSTATTT"
     gene            complement(30470..30901)
                     /locus_tag="VFK00_05165"
     CDS             complement(30470..30901)
                     /locus_tag="VFK00_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592377.1"
                     /translation="MKSKKWLAGFAVGAMLASGGAMAAVNADHVHIALSSLRLALVKA
                     SVANTMLAGTTPSSNEQAHVAAPDKLVDEEIRAIIVSEGGVINVYLTPRAGVTGGILQ
                     LVPAIGTTKGGEKDVQFTCYSPNIPDIATAAPECTFHPPAK"
     gene            complement(31045..32217)
                     /locus_tag="VFK00_05170"
     CDS             complement(31045..32217)
                     /locus_tag="VFK00_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7592378.1"
                     /translation="MPHAPLNPLDLYDVRAELSDEERMVQDSVARFVDDKVLPIIGDC
                     FDQARFPTELIPEIASLGLLGATIPEEYGGAGMNQVSYGLICQEMERGDSGLRSFASV
                     QSSLVMYPIFAFGSEEQKREYLPKMAAGEVIGCFGLTEPHGGSDPANMKTVAKQDGGD
                     WVINGSKMWITNGNLAHVALVWAKTEDGIRGFLVPTDTRGFAAQEIHKKMSLRASVTS
                     ALFLDDVRVPASAMLPNVKGLRGPLSCLDSARFGISWGPVGAAQACLKEVLDYTQERQ
                     LFGRPLAANQAIQLRLADIARRITTAQLLALRLGRLKDAGRLHATQVSMAKWNNVRMA
                     LDVARECRDILGGAGITTEHVAIRHALNLESVVTYEGTQTVHQLVVGRELTRINAF"
     gene            complement(32345..33310)
                     /locus_tag="VFK00_05175"
     CDS             complement(32345..33310)
                     /locus_tag="VFK00_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HET7592379.1"
                     /translation="MATPPPHDEKATRDNKLVVAISSRALFDLTESHALFEREGLEAY
                     RGYQIAHENDVLGPGVAFPVVKKLLALNRPEPDVPYVEVILLSRNSGDTGLRIFNSIQ
                     HYGLAITRAAFTSGEATSEYIAPFAVDLFLSANAENVARALHSGVAAATILPSQMHDA
                     HPEQLRIAFDGDAVIFSDDSERVSQEQGIDAFHRNESERWDEPLPGGPFRGFLAALHR
                     LQAAFPPENSPIRTALVTARSAPAHKRVILTLRQWGVRIDEALFLGGRDKGPFLEAFG
                     ADLFFDDSPINVESARRHVATGHVPHGVSNSRAQEMGSGGAKNGR"
     gene            complement(33402..34526)
                     /locus_tag="VFK00_05180"
     CDS             complement(33402..34526)
                     /locus_tag="VFK00_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7592380.1"
                     /translation="MTLGILIAVVVIALVFTYINGFHDTANSIATVVATKVLSPGQAV
                     LLAATTNLAGALLGSAVAITIASGLIDAGVLQVGAEVLICALLGATIWNLITWWLGLP
                     SSSSHALIGGLCGAAFAASGGNIRAIIWASDPSAWWRGDGVFPKVIVPMVVSPIGGFV
                     LGIVVMGLLLALFAWFCNRTGFLRRLGRTPFVNFFFGKAQLFSAAAMGISHGLNDAQK
                     SMGIIALALASATAAHNFDHLPSWLHFLRVSDVGGQFSIPVWVKVLCALVMAAGTAGG
                     GWRIIKTLGHKMVKLHPINGFAAETSAAAVIIAASVFGLPVSTTHVVSSAIMGVGASK
                     RFNAVRWTVVERMVWSWILTLPISAIVAWGLVEISRPLWA"
BASE COUNT         5848 a        11701 c        11483 g         5541 t
ORIGIN      
        1 cacggacaat cggtacgatc agcgggtgac ccgaaggcgc aggccgaagg agacgagcag
       61 gatgatgagc catgccgcag cacgcacacc acaacgcgcc gcccgggctt atcaggaccg
      121 gatcctgccg gcggtttcgc gcaccttcgc attgacgatt ccgcaactcg acccgcgcct
      181 gcggacggtg atcgccaacg cctatctcct gtgccggatc gcggacacga tcgaggacga
      241 gcagtcgctg gctgccgacg tcaagcagca attccatgac cgcttcaccc gcgtggtggc
      301 atccacggaa gatcccgctt cgttcgcgcg tgacctttct ccgctgctga atggcaacac
      361 cctcgccgcc gaacgagacc tcgtggagca tgcgccggcg atcatcgaga tcaccgacag
      421 tttcaacctg cgccagcgcg aagcactgac gcgctgcatc cgcacgatgt gcaccggcat
      481 gccgagcttc gagcgcgagg cgagcacggc cggactggcc gacatgcgcg agatggaccg
      541 atactgctac tacgtcgccg gcatcgtcgg ccagacgctc accgaactct tctgcgacta
      601 ttcgccgcgc atcgccgagc atcgcgaagc catgtcgtcg ctggatgtgt cgttcgggca
      661 aggcctgcag atgaccaaca tcctcaagga cttctgggag gaccgcgatc gcggtgtctg
      721 ctggctgccg cgcgacctgt tcgcggaaga aggcgtcgac ctcaagtcca ttcgccgcaa
      781 cgacgtgccg gccgcgttcg ggcatgccta tgcgcaactg atcggcgtcg cccatagcca
      841 tctgcggaac gcgctcaact acaccttgtt gatcccggag aaggaggtcg ggatccgccg
      901 cttctgcctg atcgcagtgg gactcgcgat gcgtaccctc gcctcgatcc tggccaagcc
      961 ggatttcacc gccggcgcgc aggtgaaggt gtcacgccag gtcgtcaaga aggtggtgat
     1021 cgcaacgcac ctgttcgcgg gctacagccg ggtgttgcgg cgctggttcg cgcggctcgc
     1081 gtacggcctg ccgttgcagg cgctggcgga ggactggggc gggctgctgc ccggcccgca
     1141 ttcggcctgg ccgcgcagcg atgcctgcgg gcccgcccac gctgtgacgt ccaaatagcc
     1201 atggcccccg attccggaat cccgcacgga cgtcccgccg cacacgccgg catcaccacc
     1261 cgcgagcgga cgccggggcc tctcgccgcg gctatcgact ccgcgcgttc cagcctcctc
     1321 gcgcaccagc acgcggaagg ctactggcag tacgagctgg aagccgactg cacgatccct
     1381 gccgaataca tactgatgct gcaccatctc ggcgaatccg accgcctgct cgaatcgcgg
     1441 ctggccgctt atctccgcga ccaccaacac cgcgacggcg gttggccgct ttaccccggc
     1501 ggcgcgatgg acgtgagttg cagcgtgaag tgctacttcg ccctgaagct cgcgggcgac
     1561 cggccggacg cgccgcacat ggtgcgggcg cgctcggcga tcctggagcg cggtggcgct
     1621 gcgcgagcca atgtgttcac ccgcatcgcc ctggccctgt tcggcgagct gccctggcgc
     1681 ggcgtgccgt tcgtgccggt ggaaatcgtg ctgctgccga agtggttccc cttcaacctg
     1741 tccaaggtgt cgtactggtc gcgaaccgtg atggtgccgc tgtcggtgct gacatcgctg
     1801 aaggcgcgcg cggcgaatcc gcgcggcgtg catatccgcg aactgttcgt cactccgccg
     1861 gaacaggaac ggcactactt ccaggcgcgc tcgttcctga atcgactcct gttcgcgtgc
     1921 gagcaaaccc tgcgccgcgg cgagcggttg attccacggc ggatccgcaa gcgcgcgctc
     1981 aagcgcgcgg aagcctggat cctggagcgt cgcaatggcg acggcgggct gggcgccatc
     2041 ttcccggcga tggtcaatgc ctacgaggcc ttggcgttgc tcggctaccc ggccgacgac
     2101 cctgcacgca aggaaacccg caaggccatc gatgacctgc tggtcgtccg cgaccatgag
     2161 gcatggtgcc agccatgcgt gtcgccggtg tgggataccg ggctcgcctg cctggcgctg
     2221 caggaagaca agcacgcgcc gggcgaaccg gtccggcgtg ccctcgattg gctcgccgaa
     2281 cgccagctct gcgacgcgcc gggcgattgg cgcgagtacc ggcccaggct tccgggcggc
     2341 ggctgggctt tccagttccg gaacgatgcg tatcccgacc tcgacgatac cgcggtggtc
     2401 gcacgcgcga tgcaccagtc gccggaccgc gcgcgccatg cggaagccat cgcccgcgcg
     2461 gccgactggc tggtcggcat gcagtcgcgc aacggcgggt tcgcggcgtt cgacgccgag
     2521 aacgaccacg gttggctcaa ccagattccc ttcgccgatc atggcgccct gctggatccg
     2581 ccgaccagcg acgtcagcgg ccgcgtgctg acgttgctgt ccatgctcga ccgccccggc
     2641 gaccgcgaat cccgcgaccg ggtgctccgt ttcctgctcg acgaacagac cccggacggt
     2701 gcctggttcg gccgctgggg caccaactac gtctatggca cctggtcggt gctggtggcg
     2761 ttgcgcgcgg ccggcatgcc cgtcgatgct ccccggatcc ggcgcgcggc gcgctggctg
     2821 aagtcggtcc agcaggccga cggtggctgg ggcgaaggca acgacagcta cctcgatccg
     2881 cggctccgcg gtcgcggcgc acgcagcggc cccacccaga cggcctgggc gttgctggcg
     2941 ctgatgcagg ccggcgaagc ggacagcccg gcagtcgcgc gtggcatcga ttacctgctg
     3001 aagacccagg tcgacggcga atggcgcgac acgcgcttca acgcacccgg ctttccgcgc
     3061 gtgttctatt tgaagtacca cggctacagc cgctacttcc cgtattgggc gctggtgcgc
     3121 taccgcaatg tccgtggcaa gggctgaatg cgtcgggctg gtcgtcgcgc tgcccgccga
     3181 agcacgcagc ctcggggcgc cgcggctgca tcccggtgac tgcgcgccct ggcagcacgg
     3241 ctgggtggct gtctccggaa tcggtccgca caacgcgatg cgggcggcgg agcgcctgct
     3301 cgcctgcggc gtaagccaac tcgcgaactg gggcgtcgcg ggcgcactcg acgacagcct
     3361 cgcacccggc gacatcgtgg ttcccgagtg catccggcac gcaccggatg atcccgggtt
     3421 cgccaccgat gcggtgacgt gcgacaggat cgtggcggcg tgttccgcga ccttgcccgt
     3481 gcggcgcggc gcgctgtggt cgtcggacac accgatcacg aacccggcgg acaagcgcgc
     3541 gctcgccgag cgctcgggcg cgatcgcggt cgacatggaa gcggccgggg tcgccgccgt
     3601 cgcggcccgc gcagggctgc cgttcgctgc ggtcaaggcg atctgcgatc cgctggggcg
     3661 tgaactgccg cgacggatca tgcgcgcgct cgacggcggc agcggcgggc cgtcctggcg
     3721 gatgctgtcg gcgatcgcat tcgggggtcc accggtgtgg agcgcggcgc ggggactcgc
     3781 gcaggatttc tcgcaggcgc ggcgcgcgct gacctcggcc gcgcgccttg ccgcgcgggc
     3841 cgcggtcgcc gcatgagcgc ccgcgtcctc gtcagcggcg ccaatggatt cgtgggatcg
     3901 gcggtttgcc tcgccctgtt gcggcacggt ttcaccgtgc gcgcgctggc gcggccgacc
     3961 agcgacatcg ccagcctcga tgggctggac gtcgagattg cgcgcggcga cctgctcgag
     4021 cccccgacgc tgcggcaggc gttgcaaggc tgcgaggggt tgttccatgt cgccgcggat
     4081 taccgcctgt gggcgcgcaa tcccgaagag atccgcatga acaacgtgcg cggcacccgc
     4141 aacatcctcg cggccgcgcg cagcaccggc gtcgcgcggg tcgtgcacac ctcgagcgtc
     4201 gccacgctgg ggctgcatga agacggcacg ccatccgacg aggaaacgcc cgcgagttac
     4261 gccgacatga tcggcgagta caagcgctcg aaatacctgt cggaaaaaat ggcgcggcgc
     4321 cgcgcgcgcg ccggacagga catcgtgatc gtgaacccgt ccgccccggt ggggccgcgc
     4381 gaccgcaagc ccacgcccac cggccagatg atcgtcgatg cggcgcgcgg caagatgccg
     4441 gcctacgtcg acacggggct caacatcgtg cacgtggacg atgtcgccga gggccacgtg
     4501 ctggcgttcc agcgcggcga gcgcgggcgc cgctacatcc tcggcggcga gaacatgagc
     4561 ctgcgcgaga tcctttcgac ggtcgcggaa ctgaccgggc gcgcgggccc gagggtccgg
     4621 ttgccgcacc gcgcgctgtt gccggtcgcg cgcgccgccg aggcctgggc gcgggtgcgc
     4681 gaccgcgcac ccaccatcac ggtcgacggc gtcaggctgg cgcagcacaa gatgtatttc
     4741 accagtcgtc gcgccgaaac cgaactcggc tatcgtgcgc gtcccgcccg ggaggcgttg
     4801 tccgacgcaa tacaatggtt ccgggccaac ggatactgcc gatgacatcg ccacccgggc
     4861 gggcgaccgc cctcgcgcat cccaacattg cgctggtgaa atactggggc aagcgcgacg
     4921 atacgctgaa cctgccggcg tcggggtcgc tgtcgatcac gctcgacgga ttgcacacgc
     4981 gcacctcggt ccgtttcgat cccgcactcg gcgaagacga catcgagttg aacgggcgct
     5041 gcgacgaggc cgagtcgcga aagatttccg cgttcctcga cctgctgcgc acccgtgcgg
     5101 gcatcgccac ccgcgcacgc gtggccagcg cgaacgactt tccgaccggc gccggcctcg
     5161 cctcctcggc gtccggtttt gccgcgctcg tggtggcggc cgaccgcgcg ctcggaatgg
     5221 gtttggacgc atcgtcgctt tcgcggctgg cgcggcgcgg ttcgggctcg gcagcacgtt
     5281 cgatcttcgg cggtttcgtc gaaatggcgg ccggcgcgcg cgccgacggc gaagacgcct
     5341 ttgcgacgcc gctgctcgat ccggaggcat ggccgctgag cgtggtgatc gcgatcacga
     5401 cgcgcgccgc gaaagccacc agttcgcgcg tgggcatgga cctcacgcgg cgcacgtcgc
     5461 cgttctaccg cgactggatc gcgggcggcg cacgggacct cgatgctgca cgcgctgcgg
     5521 tgcgggtgcg cgatttcgac aagctggccg acgtcggcga agcgagttgc ctcgcgatgc
     5581 acgcggtcat gctctccacc cgccccgggc tggtctactg gaacggcgcg acggtcgaat
     5641 gcatgcggcg tgtgcgcgcc cttcgcgaca ccgaagggct gggcacgttc ttcacgatcg
     5701 acgccggtcc gcaggtgaag gccgtttgcc tgccggaagc ggcaggccgc gtcgccgatg
     5761 cccttcgcga gatcccgggc gtcgaggacg tgctcgtcag cggattgggg cacggcgcgc
     5821 gggtcgtcga ggagcgcgcg gtggaggatt gtgcggccgg ggacgacggc tgatgcacgt
     5881 caccgccagc gcgccgggca agctggtgct gctcggcgaa tacgcggtgc tggaaggcgc
     5941 gcccgcgctg gtactggcgg tggatcgccg cgcccgcgtg gcactcactc ccaccgccgg
     6001 ccccgcatgg gaaatcgtct cgcccacgct cggcgccgaa gcatcgctgc gcttgggtat
     6061 cgatggcgtc gaatggcacc atgcgccggt gcccggactg gaatggctgg cggcgctgtt
     6121 cgcgcaactg ccgttcgcgg ccacgctccc gccctgccgc gtcgaactcg acagcgacgc
     6181 gttcttcctc gaccacgccg atgggcggat gaagctcggc ctcggctcca gcgccgcgat
     6241 cacggtcgcc ctcatcggcg cgttgcacgc gtgcgcgggg cggcccgacc cgacactcga
     6301 ggaagccatc gcggtgcacc gcgcgatcca gcatgggcgc ggcagcggca tcgacatcgc
     6361 cgccgcgctc gcgggcggac tctcgcgctt cgagctccgc cgaggcgccc ccggctatgt
     6421 gccaatgcgg ctcccgcatg gactgcactg gtgctgcgtg ttcagcggcc ggccggcctc
     6481 caccgccgac atgctggcaa ggatcgcggc gtggcgcgag cgcgagccgg cggagtacgc
     6541 ccgccgcatc cgcgaactcg ctacaatggc tttacgcggt gtcgacgccg ttgccggccg
     6601 cgatgccgca tccttcctgt ccagcttcga agaatatgcg cacgcactcg cgcgcttcgg
     6661 cgaggccggc ggcgtggaca tcgcgagccg cgggcatcgc gtcctcgctg cccttgccgc
     6721 ggactgccgg gtggtctaca agagctgcgg cgcgggcggc ggtgacgtgg gcgtcacgtt
     6781 cgccatggat gatatgcggt tgcaggaatt cgccgggcgc gccaccgaag ccggattcgc
     6841 cgtgatcgaa ctggatgcgg acccgcgcgg tctggaaacg aacgttgccg attgaagctt
     6901 cgaggaacaa gcatggatca gtcgagaatg ccgggtttct acaagttgtc ggtgccagag
     6961 cgcgtcaacc tcgtgcgcga gcgcggcatg ctttcgcgcg aggacttcca gaccctgttg
     7021 agcggcggcc acacgcttac ggtcgcgcgc gccgacaaga tgatcgagaa cgtcatcggg
     7081 gtgatgggcc tgccgctcgg gctcggcctc aacttcctgg tcaacggcaa ggaccgggtg
     7141 gtgccgctgg tggtcgagga accctcgatc gtggcggcgc tgtcctccgc ggcgaaggtg
     7201 gtgcgggcag cgggcggctt caccacgcgg tcgaccgaac cgatcctgat cggccaggtg
     7261 cagatcgtcg acgtcgacaa ttctccggca gcgcagaatg cgctgctgca gcgcaagcag
     7321 gagatcctca acctcgccaa cagcctgcat cccaagatgg tcgcgcgcgg cggcggtgcc
     7381 cgtgacatcg aagtgcgcat ccacccagcc gccgacggcc gccgcgacat ggtggtgctg
     7441 cacctgctgg tgaatacctg cgacgcgatg ggcgcgaaca tggtcaacac catgtgcgag
     7501 ggggtggcgc cgctggtcga gggcatcacc ggcggcaagg tattcctgcg gatcctgtcc
     7561 aacctcacgg accgcgcgct ggtatgggcg aaggccgtga tcccgaccaa gctcctcgcg
     7621 ggcaatggct acgacggcga acaggtgcgc gacggcatca tcctcgccaa cgacctcgcg
     7681 gtgtgcgacc cctaccgcgc cgccacccac aacaagggca tcatgaacgg cgtcgacgcc
     7741 gtcgcgcttg cgaccggcaa cgactggcgc gcgctcgaag ccgccgcgca cgcctacgcg
     7801 gcgcgcggcg agcactacac ctcgctgacc cgctggtaca aggacgacga aggcaacctg
     7861 gtcggcaaca tcgagatgcc gatgaaggtc gggaccgtcg gcggcaacct gcaatccaac
     7921 gcgaccgtgg cgctcaacct gcgcctgctc aaagcccaag gcgcgcgcga gctcgccgag
     7981 atcatgggcg cggtgggact cgcacagaac ttctcggcgc tgcgcgcgct ggtcaccgac
     8041 ggcatccagc agggccatat gacgctgcac gcgcgcagcg tcgccgccac ggcgggcgcc
     8101 gccccggaaa tcttcgacac ggtcgtcgaa cgcttgctcg acagcggaga gatcaaggtg
     8161 tggaaggcca aggagatcat cgcggaagtc accgaccagg cgcagcccga agcgaagacc
     8221 gtcgcgcacg gcttcgagcc ggtggacgag gccgagtacg cgaccggcta cggcaaggcc
     8281 atcctgctcg gcgaacacgc ggtggtgtac ggccgccacg cgatcgcggt gccggtgccg
     8341 atggcgatcc gcgcacgtgc cgaggaatcg aaggacggca cccagctcat catcccgcgc
     8401 tggggtgtcg agcagcgcct ggtgccgggc accaagcatc ccggttcgct gctgcagtcg
     8461 ctcgacctga tcctgcagaa gctcggactc acgaagcggc cgctgcgcat ccaggtttat
     8521 cccagcctgc cgcgtgccaa cggcctcggc ggctcggcgg cgctcgcggt cgcggtgata
     8581 cgcgcgctgg accggcattg cgcactcggc ctcaccgaca gtgaggtctg tgcattgtcc
     8641 tacgaatgcg agcgtgtcgc gcacggcacc ccgtccggca tcgacaacac cgtggcgacc
     8701 tacgggcaac cgctgctgtt ccagcgcggt gaacccccgg tcgtcgaacc gctgcacttc
     8761 gccaagccgc tgccgatggt ggtgggcctg tcgggggtgg agagcctgac cgcgaaaacg
     8821 gtacggtgcg tgcgcgaagc ctggcagcgc aaccgcaagc tgtacgaacg catcttcgac
     8881 gaaatcgacg cactcgcgat gcagggcatc aaggccctgg cggcctggga cttgcagcag
     8941 ttcggcgaac tgatgaatat ctgccagggc cagctcaatg ccttgcaggt atcgagctgg
     9001 gaactggagg agatgatcca ggtcgcgcgc gcgaacggcg ccgttggcgc caagctcacc
     9061 ggcggcggcg gcggcggcgc gatgatcgcg ctctgccccg acaatcccgg ccgcgtcgtg
     9121 cgcgcgctgc aacaggccgg ctaccaggcg atggaaacga acattggctg acgacgcaga
     9181 caaccgggtg gtttcgttcg agagcgaacg gctgatcctc gtggactcgg ccgatcgcga
     9241 gatcggccac gccagcaagg ccgacgcgca cgccggacag ggcatcctgc accgcgcgtt
     9301 ctcgctgttc gtgttcaacc cggcgggcga gttgctgttg cagcagcgcg ccaccggcaa
     9361 gcccctgtgg cccggctact gggccaacag ttgctgcagc cacccgcgtg gtggcgagga
     9421 catggaaacc gcgacccgtc ggcgcctcgc cgaggaactc gggttcacct gcccgctcga
     9481 attcgtctac aagttccaat accacgccga gtacggcgag gccggttcgg aatcggagtt
     9541 gtgctgggtg tacgtcggca cgtccgacgc gccggtgcgg atcaacgaaa ccgagatcgc
     9601 ggcctggcgg tacgtcgcgc ccgatgcgct ggatcgcgaa atcgcggaag cacccgaccg
     9661 cttcacgccc tggctgaaga tggaatggca gcgtctgcgc ggcgaattcg ccgagcgcct
     9721 gccgggcgcg cgcgttgcac agcccccctc caggacgcgc tcgttcgcga cggattgatc
     9781 ccggaacgat acggaagtgc ccgcacgcgc cggggacaat caggccaccc ggtcaggaaa
     9841 ccatcgacgc aggcgacgct tcagcgccaa tgccggcgcc tccactgcgt gccaggaaca
     9901 ccacgccagc gccgccgtcg cgaccacgga caccaccagc gtgccggcga gcgacccgtt
     9961 ggcaggccag taccgcacgc tgagctgctg cacgaagtag gcatagatgt aaataccgta
    10021 tgacaggtcg ccgaagcgcc cggcggaccg gatccccggc gtgtccatcg aacccgtgag
    10081 gagcaccagc ggtgcgacga tgacccaggt cgccaggtcc tggcggccgc tcgccacggc
    10141 tgcgaggaac gcggccaggc cggcgcatga ccacgcgatg cgatgccgca ggacttctgc
    10201 cgtgaactgc cgggcgcaaa ggcccgtggc aaaaaacagg atgtaaagat acttcaggcc
    10261 ctggcccgtc ggcgactgca aatacaggaa cgcgacgact gcaaaggcga gcacggaaag
    10321 ccagcgccgc ctcaggccga cgacgccgag gagcgcaagg tacaggtagc acttcgcttc
    10381 gacgggaatg gtccaccaac ttccattgac cgccgccatc gcggcgccgg aaccacccgc
    10441 gaaaacgccg ggcaattcgt acacgatgcg cagctgcagg ttgcgcgcga cgaaatgccg
    10501 gacagcgccg aggtaatcac ccgcggccat gctggtgagg aacaccgaag ccgccgcgat
    10561 cacgatcgtg gcggccgcga gccccggcca caggcgcagg aaccggcgca ggatgaatcg
    10621 caccaggtgg ggatcgcgat accagctctc cgcgaccaga tagccgctga tgacgaagaa
    10681 catcatcacg gccagttcgc ccagcgtgta gccgccgacc gtcggctggg ccttgcccag
    10741 gaagaacaac tggtgcgagc acaacacgag cagtgcggct accagcctga tcgcgtcgaa
    10801 gttgttggtg cgcgcccgga ccgtcgcagg cgccgcatcg cccaccgccg cgccggccgc
    10861 gtccagatgc aatccgaccg cagcttcacc gtcgctcgac atgttcggag gaccgcctgt
    10921 ggagtagtgg acgagggacg aacgttccgc gcgagcaacc gatagcgcgg tgggctgcta
    10981 gagtggccgg aacgatcgca cgacgggtgg catggtagcg ccacgggcgc gacgcgtgca
    11041 ccccgttcac gagtagccac gggctgccgc tggcgcgtca acgccacgtt gacacgtgcc
    11101 ggtccactat ccggggcagg cccaccgacg cgcaaccgcc cgccggtcac accccaggcg
    11161 ctccggcgcc agaggagtca acgatgtcca ttcccgtccg tcaacaactc aaggtcgcga
    11221 gctacatcat cggccaaaag ctgcgcggcg cgaagcgcta tccgctgacg ctgatgctgg
    11281 agccgttgtt ccagtgcaac ctcgcctgcc ccggctgcgg caagatcgat tacgactcgc
    11341 acatcctgaa gcagcggcta tccgtcgagg aatgcatgca ggcgatcgac gaatgcggcg
    11401 ctcccatggt gtcgctcgcc ggcggcgagc cgctcgtcca caaggacatc aaggaaatcg
    11461 tcgacggttt cctcgcgcgc ggcaagtaca tctacctgtg caccaatgcg atcctgctcg
    11521 agaagaaact cgacctgttc acgccatccg accacctgac cttctcggtg cacctcgacg
    11581 gcatgcgcga acggcatgat gaaagcgtcg ggcgcgaggg cgtgttcgac actgcggtgg
    11641 ccgcgatccg cgaagccaag caccgcggat tccgggtcaa cataaacacc acgctgttca
    11701 ccaccgccaa gccggacgag gtcgccgaat tcttcgattt cgcgacctac gacctcgggg
    11761 tcgacggcat cacgttctcg ccgggcttcg cctacgagca cgcgccacgc caggacgtgt
    11821 tcatgggccg gcgccaatcc aaggagctga tgcgcaagat cttccgtcgc aggaaggaaa
    11881 cgcgcagcaa gtgggcgctg tcgcaatcga gcctgttcct ggatttcgtg tgcgggaacc
    11941 agggctacca gtgcaccgca tggagcatgc cgtgccgaaa cgtgttcggt tggcagaagc
    12001 catgctacct gctggtggac gaaggctacg tgccgacgtt caaggacctc gtcgagaaaa
    12061 ccgattggga caattacggg gtcgggcgca atccgaaatg cgcgaactgc atggcgcact
    12121 gcggcttcga gggcaccgcg gccgacgatt cgttccggca tccgctgaag gctttctggg
    12181 cgagcgtgcg tggtccgaag ctgagcggcc cgtacgcggc cgatccaccg atcctgtacg
    12241 acacaccgga accgaaccag cggagcccgc agagcaacgt cccgatcgca cactttggcg
    12301 gcataaggaa ctgaccccca gacgctgcca cgcgggtgcc acatgctgat cctcgccgcc
    12361 gcctcgttcg ccctgtggct ggcggtgttg ctggtcccgt ggcagccatg gcgctgcaag
    12421 gaaaggctgg aagccgatgc cgccgacgat gcgcaagcgt cgggcttcac ggtgctgatg
    12481 ccggcgcgca atgaagcggc ggtactggaa gagacgctgg cggcgttgcg cgcctgcgcg
    12541 ccggccgcgc cggtagtcat cgtggacgat cagtccagtg acgcgaccgc ggacattgcc
    12601 cgcggcagtg gtctcggcaa cctgcggctc gtaccgggca cgccgccacc ggccggttgg
    12661 gcgggcaagc tctgggccct ggaacagggc ctccagcacg tctcgacgcc ccgcgtgctt
    12721 ttgctcgatg cggatatccg gctcgcgccg ggcatgcccg ctgccctgca gaagaaggcc
    12781 aacgaaggct acgcgttggt atcggtctgc gcccagccct gctggaccgg tgcggccgcg
    12841 cgctggctgc tgccggcgtt cgtgtatttc ttcaagctgc tgtacccgtt ccggctggcg
    12901 aaccggagcg attcccgagt cgccgccgcg gccggcgggg tcgtcctggt ggaccggcgg
    12961 gcactcgccg atgcgggcgg cttcggcgca tggcacgacg ccatcatcga cgactgcacg
    13021 ctggcagcgc acgtcaagcg tgcaggtcac cactgctgga tcggcctcac ccgcggcgcc
    13081 acgagcctgc gccgctcgga tgcaggcgac atcgcccaca tggtcgcgcg ctcggcattc
    13141 gtgcagttgc gcgaatcggt catcctgctg ttggtggcga cggcgctgct ggtgctggca
    13201 ttctggatcc cggtgctggc ggtcgccacc ggcgacggcg cggcgcgctg gctgggcctc
    13261 gcggcctggc tggtgctgat cgcctgctac ctgcctacac tcgcgtacta tcggcgcaac
    13321 gggctgaccg cggtactgtt gcccgccacg gccaccttct ttcttgccgc aacctggttc
    13381 tcggcttggc gcgcactggc cggcacgcgt tcggtgtgga aggatcgccg ctaccagcgc
    13441 gggaccggtt gaatgcagaa tcgcgcagag ccagcgtctt ccgtccgcat gccgcaggcc
    13501 ggggcgattc ccgatgccgg cccgcatccc ctagcgagct ggctgttgca attcgcggcc
    13561 gatctgtaca tcacggccgt gtaccaccgc gaggtcatcc tgccgccggg cttccgcatg
    13621 cggccgggct cgatcattgc gtccaaccac caacgtgatg tcgatggccc ggtgctcggg
    13681 acggcgctgc tggatcgcca cgggctgcgc ttcgcgggcg tattgcccta tttcgtgacg
    13741 cgcgaggatc tgttccgccc cggcatcctg gcgcggctcg cggtgcactg gccgcgcgcg
    13801 ttctccggat tgcttcggcg gatctcgctg gcgtggtttt tcccgctggg ccgcaccgag
    13861 ccgatgcgac ggacccgcga attcaccctc ggcgatgccc tgcgcgcact ggttgcggaa
    13921 ggactcggcg atgccgacgc gggttcgctc ctgaacgcgc gcgggcagcg ggaaaccggc
    13981 gcccggcctg gcgaactcac ggtcgacgca ctgctcgagc gcgacgatga tgcgctggag
    14041 caatggtggg gtctgcgcag gttttcccgc ccggcgcgcg ccaccatcgc accgggcttt
    14101 cgcgcgacgg tcgagcggca gatcgcccat ttcgcggcgc gactcgacca aggctgctgc
    14161 atctacttcg caccggaagg cacgatctcc acgcacggtc cattcggccg tattcgcggc
    14221 ggcttctacc ggatcgcctg cgcgaccgaa tcgccgccct ggatccagcc gatcgggctc
    14281 ggctacgaca cgctcgcgcc ggggaagtcg cgggtggtga tccggatcgg cgaggcattc
    14341 cgcgccgaca cccgcatgcg ccgatgcgag ttcgatgcga agctgcgccg tgccgtgctc
    14401 gacctcgtga cgatcacccc gagccacctg ctggcgcgct tcctcctgca cgggccggca
    14461 tccttttcgg tagaggaact gtcccgatgg ctggcgcaag tgcacgccaa cctgtcggcg
    14521 aacaacgcag ccgtggatcc gctgttcgaa cgcgtccgct tcgagcggat cgcggcccgc
    14581 aggctccgct ggctgcaccg caaggggctg gtggcgcggg taggcgaccg gttccgcaac
    14641 acctgcccgc gcgacctgca gccgggctgg cggcggcccg ggaatgtcgc ccgctatctc
    14701 gacaacgcgc tctcggactt cctgcccgac atcgagcggc tggcgccatg ttgaagaacc
    14761 tctggtccat gctccggcac gtgctcaagc cacaggtggt cctgccggtg ctgctggcag
    14821 ccgcgctgct ggtgttcgcc ttcaacctcg gcgatctcgg caaggtggtg tcgcggttgc
    14881 gcgacctgcc gctccacgtc gtgggcattt cgctgggctg cgcgacaatc tacctcggct
    14941 gcaaggcggc gcagctccgg ctgatgctga cgcggatcga cgtttgcatt ccgccccgct
    15001 cgttctggct cgcattcgcc gtgggcgaac tgacggtaac cctgccgctc gggctgtttt
    15061 cgcagaactg ggtgatgtcc gccgcgcggc gcgtgcacgt gggtcgcagc gccgccgcga
    15121 ccgtgatgat gctgctggtc gagatcgcgg tggtgttcct gttcctggcg gtcatcggcg
    15181 tgcccggatg gggcgaggtg cgctgggtcg cggtcgggtt gctggtgctg ttcgtggcgt
    15241 tcgtggcggc cgccgtggtg ttcgaggaca aggcccgcaa actcgccagc ggcgtgcgcc
    15301 gggaatggct gaagaaggtc gccaagggcg gtgtcgaact actggacggc ttgcgcaagc
    15361 tgtgcacgcc gcacacgatc ggggtcagcc tggtgctcgc cgccgtgtac ctcggcgcgc
    15421 tgaccgctgc gttctggcag gtgggtcgcg gcatgcacgt gcagcatctc gactacatcg
    15481 acgcctccac gatctacatg ttttcgctgg cggtgatcct gatcggcgca ggccttgtca
    15541 gccagatcgg caccgtcgac ctgctcggca tggtgatcgc tcgtgcgttc ggcatcggct
    15601 ataccgacgg cctcgcgatg atgctggggt tccggatcgt gtggaccggt tccatctggg
    15661 tcctgtgcct gccgatcgtc ggcgccacct ggcgcgaaat gccatcgcgc ggcaaacgca
    15721 agtcaggtga cggcggcaag caagagtctg gttgaggggc ttgttgccta tccgtggcgc
    15781 gaacgtcgtc gacgcgaccg cgccaccgtc ttcggatcgc tggcacgacg gatgcacatg
    15841 ggcaccgcat cgcgccacct gctccaccca aggccgccgc cgagcagtgc gaggccgagg
    15901 cacatgaggc cgaatgctcc cggttccggt acgcccacac tcacgccacc gccgccgtcg
    15961 cccccgccgc cggacggtcc gctcaacgcg agctgcaggc cgcgcccgtc gttgatctcg
    16021 ttgagctcac acccgaagcc ttgcatcagg ccgtgacacc tgaaattcac ccagtccagg
    16081 ttgaagaaag ccgatgcgct gaagaaatcg aaggtatcac catcgcgctg gctcgtgttg
    16141 ttgccgagat agaaatcgaa ccaggtggaa ccgagcccga tggcgaacgg gtcgaacatc
    16201 accgtggaac tggagacaag ggtatcgaag ctcgcgaggc tgtcgacatc caggtgcatc
    16261 gaaccataaa gctcgatgct ctgcgcgtcg atgataaaac tgccgatatc ccccggctct
    16321 tcgccgggac tgagcaccgc gccatcaccc agatgcaaga ggctggcggc cgtgacatgg
    16381 ccgctgccgg ccagcgtgcc gccatcgatg cggatcaggc cctgtgaaag atcgccttgg
    16441 agcgtcgtgg tgccagcggt ttgcgcgaag actccggaac ctcgaatttc gcccgtggat
    16501 ttcagggtgc cggcattcac gaactccccg tcattgcgga gcgtaccggc attgtcgaag
    16561 tacccggacg cccggttgtc gaatcgacca tggttcgtca ccgtgccaga cggtgcattg
    16621 gtcagcgttc catcgttggt caacgtgccc gccttgactt tcaatgtccc atgattggtc
    16681 agcgttgcgc ccgcctcatt ccgcagggtg cccacgttgg acagcgaagt gaagttggtc
    16741 aacgtcgcgc ccgactcgtt cctcagcgag accaggttcg acaacgaacc gaagttggtc
    16801 agtcttgcac ctgcctcgtt cctcaggctg cccgagttcg aaagcgaccc gacgttggtc
    16861 agtgtgcctt cgaaccgatt cctcagcgtg cccagattgt ccaggtcacc gacgttggtg
    16921 atcgtcccca tcaggttcgt cagcgtggag gcgttggtca agtggccaga gttttgcacg
    16981 gtggcctgca tgttattgaa ggaaccgaag ctcacaaggg tcccttggtt gatcaattgg
    17041 gcgccgacga gattttgcag cgtgctgctc tgcagggttc caaaattggc cagtacaccg
    17101 tgaagttgat tcacgatggt gccgtagctg gccatctggc cgaagtttct gatgtctccg
    17161 ccctggttca cgaacgaacc gctattcgtc agcacaccgt agttgccatt ggtcaacgta
    17221 ccgtagttgg agaatgcgcc gccattgatg atttcagcgt gtccccaact cgccccgtcg
    17281 ttggtcaggg tgccgaaatt ctgcacgctg ccatcgttga tcagttgggt cttcacagtg
    17341 ttccagaaca caccggtggc tgcattcagg agaatgccgc cgtcatgatt gacgaccctc
    17401 tggaagttga acacggtgcc ggcattggtc agcgagcccg gagaatggat atccaccgtg
    17461 tagagggcgc agaaggtacc aggggccacg ctcctgtcat catggatatt gatctgcgga
    17521 tacattggat tgcagtccag cggttcggag gcgcgtcccg gcgcaacgtg cagtccgacg
    17581 ctgcagacgc tgaccacgag ggccaggcgg aatgcgccgc gccgcgcgcg acggcgggtc
    17641 acgagaggcc ctggttgatc ctcgttggct ttcatcactg ctccccttga ggcttttcga
    17701 cgcgccgaaa aagcgcgaac cgatatcccc cacacacgag cagatgccgg taccaggggc
    17761 gcgaaccttc cagcgcagcc gatcgggcga cccacggccg gcctaggcaa ggctgtctcg
    17821 tttgggaagc acggtccgta ccaagcggcc aaagcatctg caatcaacac gttgcaaaca
    17881 ccagccgaat ctgatccgcg caaagcgtaa agtctaccga cagtcaggcg agatgcccgg
    17941 ggcccatgcc tcaaccgatc gctgccagga agttgccgac ggacgcggtc gtgtccatcc
    18001 gtggtatgcc ggcggctgca cgcgcgcgtt cgagatcgcg cggaatgtcc aggtgcttct
    18061 gttccaccga ccacacgtgc ccacgttcgc gatggaagcg ggcgaggtat tcctgttcgc
    18121 tctgccccgg cgtcgcggtg aacaatgccc gccggcccag gccggcgagt tccatcaggg
    18181 tggtgtagcc cgaccgggtc attaccagct ttgcgcgatt gagcatctcg gcctggcgct
    18241 tgcggtcgag gtagtcgtat acagttgcgc ccgcgatttc gcggcattcg ccagcgtgcc
    18301 ccggtcggcc gagcgtcacg acgatcctgc cggacagccg cggcagcgaa tcgagcacca
    18361 gttcctccag caggccgcgt tgcggctcga tacccgaaac cgaaaagaac acgtcgatgt
    18421 cctgctcgac atccatgcgc ggcacgctgg acaatggacc gatgtatgcc agccgccctt
    18481 cgccccagtc gaaactcggt tcgtgcccca gccagccgga caaaccgccg ttttcctcga
    18541 catccgggat caggatgcgg gtgaatcctt tcaggagatc gcgctgcccg cgctccgcgc
    18601 accattccgt gaagcggctg aagaacggca cgcgctggtg gatggagtgg aggatgcaat
    18661 agctcggcac ttcgcgggac cacacgccga atcggctgtc ggataccacg cggtcgaaat
    18721 gccgctcggc aaccagcttc tcggtgaagc gatgctcgcg acggaaaccg gccaggatcc
    18781 agggcatcgc cgcggtcatt cgaacgtaaa acaccacggg atagcggctg aacggcgcgg
    18841 gcgtgtcctg gaagggcagg tactcgcagg cgtcaccgag ctccgacttc agcaggctca
    18901 tgccgccgcc gggctgcatc aggatcgtca cctcgtcgcc gcgatcgagc aacgcgcgga
    18961 tcaagccgag gctgcgcgtc gcatgtccaa gtccccagtg atagacccca aacaacactt
    19021 tgcgtggcat tcatgatcct ttgcattcca gggctcggcc gcggctcccg gatgctcaca
    19081 tgaaaagcgt ctacgcagcg cgaaggcagg gatcatgcga gaatccccgg tccgcggcac
    19141 ctggcgtagg ttgggttgat cccggacctg ctccgggcga agcccaacgc aattgacggc
    19201 gaatacgttg cgcttcacgt cctgcaacgc gacctgctgc aaaattcacg acccggcccc
    19261 gtagctcagt tggatagagc gtccccctcc taaggggaag gtcacaggtt cgaatcctgt
    19321 cggggtcgcc atccttgcac atgctgcgcg ccgttcgggc cgcgcgtgcg gacgctcgca
    19381 ctcgtgtcga ttccgtcgcg gctcgaagcg atgcagcacg gctgttacaa tggcggattg
    19441 actcgacgac attcgcgctg cacgcaacag gagctttcat gtcccacgcc atcctttccg
    19501 cactcggcct cgccgacacc aattccggca ccttcctcgg caatggcgaa tggtcgaaaa
    19561 cgaccgatgc cggcgtgctc gagccggtga atcccgcgac cggcgagccg ttggccaagg
    19621 tgtacgccac cagcgacgcg gactatgaaa ccgtgatcgc caacgcgagg aaagccttcg
    19681 aggaatggcg caagaccccc gcgccgcggc gcggcgaggc gattcgcctg tgcgcgcagg
    19741 cgctacgcga acacaaggac gcgctgggtt cgctggtcgc gctggagatg ggcaagatca
    19801 agcccgaggg cgacggcgaa gtccaggaga tgatcgacat cgccgacttc gcggtcggcc
    19861 agtcgcggat gctgtacggc aacgcgatgc attccgagcg cccgggccac cggatgtacg
    19921 agcagtggca cccgctcggg ctgatcggca tcatcagtgc gttcaacttc cccgtggcgg
    19981 tctggagctg gaacgccttc atcgccgcgg cctgcggcga cgtatgcatc tggaagccgt
    20041 cgccgaagac gccgctttcg gcgatcgcga gcgtccggat ctgcaacgag gcactgaagg
    20101 ccggcggatt cccggagatc ttctacctca tcaatgacgc cggcaacgag ctcgcgcaga
    20161 agttcgtcga cgacaagcgc atcgcgctga tcagcttcac cggttcgacc aaggtcggcc
    20221 gccacgtcgg cgagcgggtc gccgcgcgca tgggccgctc gctgctggaa ctcggcggca
    20281 acaacgcgat catcctggat cgctcggcgg acctgaaact cgcgatcccc gcgatcgtgt
    20341 tcggcgcggt cggcaccgcc ggccagcgct gcaccagcac ccgccgcctg ttcgtgcacg
    20401 agtcgatcat cgacgacgtg ctggacaagc tgcgccacgc ctaccagcag gtcgagaaga
    20461 agatcggcga tcccaccgac cctgccaccc tgatgggtcc gctcaacagc aaggcggcgg
    20521 tcgagcagtt cgccaacgcc gtgaaaacgg ccaaggccgg cggcgcgcgc gtcgtcagcg
    20581 gcggttcggt ccttgagcgc aagggcaact tcgtcgagcc gaccatcatc accggcgtga
    20641 agaattccga cgagatcgtg cagaccgaga cgttcgcgcc gatcctgtac gtgatgccgt
    20701 tcaagacgct ggacgaggcg atcgagatgc agaacgacgt gccgcagggt ttgtcgagcg
    20761 cgatcttcac ccagaacctc aaggccaccg agcaatacct ttcggccatg gggtccgact
    20821 gcggcatcgc caacgtcaac atcggcacct cgggcgccga gatcggcggg gcgttcggcg
    20881 gcgagaagga aaccggcggc ggccgcgagt ccggttcgga cgcatggaag gcctacatgc
    20941 gccgccagac caacaccatc aactggtcgg atgcgctgcc gctggcgcag ggaatcaagt
    21001 tcgatatctg acggcgcagg caaaagccat tccctcccgc gctgcgcgcc cgttcgcttc
    21061 gcttcggaac gaagggggaa gagaaccgcc cccttccgca ggaagggggt cgccgcgcag
    21121 cggcgggggg atgactccgg aggcgaaggc gcatcatcgg cgatcggagc aatcgtatgc
    21181 acaacccttt cgacttcgcc ggttaccgca tcgccatcgc cgggggcagt cgtggcatag
    21241 gccgcgcaat ggcgctgggc ttcctcgaag cgggcgccca ggtcgcggta tgcgcgcgcg
    21301 ggcaggaagg cctcgacgcg ttgtgcagtg atgccggcga gtacgcaaag aaactccaca
    21361 cgcacacctg cgacctcgcc gacgccgacc aaatcgaaca ctggatcggc gacgctgcgc
    21421 gcgtcatggg cggcatggac gttctcgtca acaacgccac cggctatgga ttccgtgacg
    21481 acgacgaatc gtggctggtc gatttcaatg tcgacctgat ggccgccgtg cgggcgtcgc
    21541 gccatgccct gccccacctc cggcgttcac cgcacccgtg catcctccac accagttcga
    21601 tcggctcgct gcggccgcgc acgatgggac cgccgtacgc agcgatgaag gccgcgttga
    21661 accattacgc agccacgcaa gcgctcgcac tcgcgggaga tcgcatccgc gtcaacgcaa
    21721 tcgcacccgg ctcgatcgaa ttcgaaggcg gcgtgtggga ccaggtcaag cacgcaaagc
    21781 ccgggcaata cgcgcaggtg ctggcgacga ttccgttcgg ccgctacggc acggccgagg
    21841 aagtcgcgca cctcgcgctg ttcctggcgt cgccgctggc gagctggatc accggccagg
    21901 tcatggtcgt tgacggcggg caatcgctgc gctcctgacg agcggggcgt gaccgccacg
    21961 ggtatgcgac catagcgcgg tgccatcgga ggcagttcag ccatgaatac ggtctatccg
    22021 cgcaccagtt cactggcggt cgtcagcctc atcttcggga tcctggcgta cgtcgtcctg
    22081 ccgggtgtcg gtgcagtggt ggcggtgatt tgcgggcatt cggcgcgctc ggagatccgt
    22141 cgctcgcctc ccggcagcat cgagggcgac gggatggccc tggccgggtt ggtgctgggc
    22201 tggatccaga tcgcctgcgc ggtcatcgcg gtgggattcg cgatcctcat catcgccggc
    22261 gcgctcgcgt tccacggatt ccactgatcg atgtacgggg cgctgcggcc gctgctgttc
    22321 gcgctggaag ccgaggccgc gcatggcctg accctgtacg cgctcggcgt cgcgcagcgc
    22381 agcaacctga tgcggttcgt cgcgcacccg ccgccggacg acctgccggt gcgcgcattc
    22441 gggatcgatt ttccgaatcc cgtcgggctt gcggcggggc tggacaagaa tggcgaacac
    22501 ctcgatgcgc tgggcgcgct cggcttcggt ttcatcgagg tcggcacggt cacgcctcgc
    22561 ccgcagccgg gcaacccgag gccgcgcctg ttccggctgc ccgggcggga ggcgctcatc
    22621 aaccggatgg gtttcaacaa cgatggcgtc gacgtgctgg tgcggaacgt cgaacagaca
    22681 cgatggcgcg gcgtgctcgg catcaatatc ggccggaaca aggacacccc gaacgagcgc
    22741 gccgccgacg attacctcgc ttgcctggag cgtgtgtacc cgctcgccag ctacgtcacc
    22801 gtcaatgtct catcgccgaa tacgccgggg ctgcgcgacc tgcaggaagg cgattcgctg
    22861 cagcggctga tcgccgtgct ggtggaatcg cgcgagcaac tcgccacgcg gcatggcgcg
    22921 cgcaaaccga tcctgctgaa aatcgcgccc gacctcgatg atgcggggat ggatgcgatt
    22981 accgccgcgg tcgccggttc cggaatcgac ggcctgatct gcaccaacac cacgatcgac
    23041 cgcacgggcg tcgcgggcga accgcacgcc gacgaagccg gcggcctttc cggcaagccg
    23101 ttgttcgaac cgtccaccga cgtgttgagg ggcatggccc ggcgcctcgg cgcgaacatg
    23161 ccgctggttg gcgtcggcgg catctcgagc ggcagcgacg cagccgacaa gctcgccgcc
    23221 ggcgcggccc tcgtgcaggt ctactctggc ctgatcttcc gcggcccctg gttgctgacc
    23281 gaatgcgtgg aggagatccg gcggcaggtt ggaagttcgc atgtcgaatg acaacccggc
    23341 catggatgcc gcgtacacgc tgaccgggag tgcttcgctg ctccagcgca ataccctgcg
    23401 cgtggacgct cgtgcggcgt ggctggcgga gatccacgaa gccgatgcga ttccggcact
    23461 gttcaccgag cccgtactgc gcggcaagcc ggtgttgctg ctcggcgaag gttcgaatgt
    23521 cctgttcgcg ggcgattacg acggggcggt cgtgacgatg aaggcccgcg gcgtcgaagt
    23581 ggtttccgaa gacgccgacg ccgcgcgcat ccgcgtcgcg gccggcgagc actgggacga
    23641 cctggtgcgc tggacgctcg cgcagggcta ccccgggctc gagaacctga tcctgattcc
    23701 cggcagcgtc ggggcggcgc cgatgcagaa catcggcgcg tatggggtcg aggtcggcga
    23761 attcatcgaa tcggtcgaag cgttcgacct cggcgggcag cgcgcggtgc gtttcgacac
    23821 cgggcaatgc gaattcggct atcgcgattc cgtcttcaag cgccatccgg atcgctggct
    23881 gatcaccgcg gtgacgctcc gcctgccgcg cacgcacgcg ccccgcatcg actatgccgg
    23941 gcttcgccag gaactcgagc ggataggcgt ggcgaatccg gccgccatcc acgtcgccga
    24001 tgcggtggtg aacctgcgca cccgcaagct gcccgatccc gccgtgatcg gcaacgccgg
    24061 cagcttcttc aagaatccga ttgtcccggc accgcaggcc gccgcgttgc gaagcgatca
    24121 tccgcagctg ccgtgctggc ctggggacgg cggcgtgaag ctgtcggccg cgtggctgat
    24181 cgattcgtgt ggattcaaag gcttgcgcaa tggtgatgcg ggtgtttcga gccgccacgc
    24241 gctggtgctg gtgaaccacg ggcgtgcctc cggcgccgag ctctgggctc tcgcgcggca
    24301 tgtgcgcgat ggggtacgcg aacgtttcgg cgtcaacctg gagcccgagc cgcggatcat
    24361 cggcgcgccg gcctagactc ggggcacccc cttcggaacg aagggggtcg atggtgaccg
    24421 ccgcaagcgg cggtcaccat cggggggatg ttcgcacacg cggtgcggcg gacggtgccc
    24481 gtcctacgat ccgtgctcgg cgccgggtac tgccatgtaa tgtcgctcgc ccttggcggg
    24541 atcgaaatac accgctgtca tgaggccggt ttcggggtcg acgaaacgct cgccagtatc
    24601 ggtccaggcc gggtccggcc gtccactgcc caccggtttg tagcgccagc gctcgatcgc
    24661 cagcgcgacg atcaggaaca cgccgccgat gatcagttgc ggcaggaaca ccaccagcac
    24721 cccgccgacc agcagtgcga tcgccgcgac cagcagcagc aacgcaccca cgacatagag
    24781 cgcgaccacc atggcagcgc tcagaccttc tcgagcacaa ccgccgtccc gtaagcgacg
    24841 atctcgctca tggtctggcc gatctcggac gaatcgaagc gcatcatcac gaccgcgttg
    24901 gcacccatcg cctgcgcgtt ctggaccatg cggtcggtgg catggcggcg tgcctcctcg
    24961 agcatctggg tgtactcgct gatctcgccg ccgacgatcg agcgcagtcc cgccatcacg
    25021 ttgccgccga tcccgcggct gcgcaccacc agcccgaaca cctggccctt ggtctcggcg
    25081 attcggtggc ctgcgacgtt ctcggtcgtg acgatgatca tggctgcctc ccgatcagat
    25141 gccggtcgcc cgcatgcagg ctacgaatgg attgtaggag cgcgccacgg cgcgcgacca
    25201 tggcacgcga caagatcggt cgcgaccatg ggccgctcct gcattcaccc ggcggcgcgc
    25261 gctcttgggg gagcgggcca tgcccgcgac attggttcac cccgccgcct gctgcccgcc
    25321 ggctcagcgc cgggtgttgc ccagcagccg cagcagttcc aggtatagcc agaccaatgt
    25381 caccatcagc gcgaacgcgc agaaccattc catgtaccgc ggcgcacccg ccgccgtggc
    25441 acgttcgatc aaatcgaagt ccagcaccag gttcagggcc gcgatgccga ccacgaacag
    25501 gctgaacagg atgccgaagg tgccgccgcc gaagacgaac gacatgccgc cgaagccggt
    25561 gaacgccgag atcccccact gcaccaaata caggagcaat atgccgccgg tcgccgcggc
    25621 cacgcccatg cggaatttat gcgtgacctt gatcacgccg ctgcggtaca ggaacagcat
    25681 caccgccagc acgccgatcg acgccatcac cgcctgcagc acgatgccct ggtagcgcac
    25741 ctcgaacagc gccgagatcc cgccgagcgc ggtcccttcg gcaagcgcat acagcggtgc
    25801 cgtgaccggc gtccaattgg gtttgaacgt gctcgcgacg gcaagcacca atccgacgat
    25861 caggccgccc atcaggaacg gcgtcaccgc ggctgcgccg gcggcgagat ccccgcccgc
    25921 cgaggcgttc atgaaatggt tccaagtcca ggcgccggtg atcagcatca gtaccagcag
    25981 caggccggtg cgaaccagcg tgccgttcaa ggtcatcggc tcggcggttg ccggcaggtt
    26041 ctggaagaac ttgtcgcgca gcacgggatt acgggaacgc agggccatgg atctcctcac
    26101 tcgatcaatc gttgccggcc gctgccggcg ttgctgcaag gttagcggac gcgcggatgc
    26161 gctgccacgc cgacttcttc agaccccgga ccgacttgcc gagttccact ggacccgttc
    26221 caggcgagac atgaagcccc gtccagggcg cgccacgaat accgatcaac cggcatcgga
    26281 ttccggggga cgcacgtccg gcaggaaggt cgccagcagg cccagcagcg gcaggaatgc
    26341 gcacacctga tacacgaact cgatcccgaa atggtcggcg accacgccga gcaccgccgc
    26401 accgatgccg ccgaggccga agctgaagcc gtagaacaac cccgacacca tgccgatgcg
    26461 atgcgtcaac atgtcctgcg cgtacacgat gattgccgga aacgcggacg caaggatcag
    26521 gccgatcacc acgctcaggc caaccgtcca gccgagtccg acgtgcggca gcagcagcgt
    26581 gaacggtgcg cagccgagga tcgagaacca gatgacccgc cgccgtccga tccgatcgcc
    26641 caaggggcct ccgatcagcg tgccggcggc gacggcgaac aggaacacgc ccaggaggtt
    26701 cgcggccgcg tgggccgaga gcccgaaacg gtggatcagg tagaactcgt agtagctgct
    26761 gatgctcgcg aggtagaaga acttcgagaa catcaggatc agcagcacgc cgatggtgcg
    26821 ccgcacgacc ggcgcgggaa acagcggctt ctcgtcgggc tggcgcttgc gcacccgctc
    26881 ctcggcgtgc cggctgtacc agcggctgac ctgcagcagg atcacgatcg cgagcagcgc
    26941 cgccagcccg aaccagccca cgtgcgagcg cccgagtttc agcacgatcg ccgccgcgag
    27001 caaaggcccg agcgcctgtc cgatgttgcc gccgatctgg aataccgatt gcgcgaagcc
    27061 gaagcgtccg ccggatgcca tccgggccac gcgcgaggac tcggggtgga acaccgacga
    27121 gccgcagccg atcagcgcca ccgccaccag cagcaccgcg taggacggcg ccacggagag
    27181 cagcaacaag ccgccaagcg tgaaacacat gccgatcggc aacgagtacg gcaatggctt
    27241 gcggtcggtg accatgccga ccaccggctg cagcagcgat gccgtcaact ggaacgtcag
    27301 ggtgatcgcg ccgatctgga aaaagccgag atcgaactcg cccttcagca gcgggtagat
    27361 cgccacgatc accgactgca tcatgtcgtt gaggacatgc acgaagctga cggcggccag
    27421 caggctgtag cgcgtcggtc cgtgcggcgt gcgcgccgcg cgcaccggcg ccaccggttc
    27481 cacggcaccg ctgccgctgg ccgggttcag gtccatgccg taagccgcag gcgcgtcctc
    27541 accacggcgg tcccgccgac gctcggaaag ctccggaaac gcacctgccg ggaatggtgg
    27601 tcatgaccgc ttgcggcgtc cgttgccgcg agccaacgtg cccgccgcct ccacgatgtc
    27661 ggcctcgcca ggaaggacca gcagggccgc gccggcgagc ggggtgtagg tatcggcgcc
    27721 caccacgcgc gcgatcggct tgtcgccgag ccccgcctcg accaacgcgg tgatgatccc
    27781 ctcgcccacg cctgcgctcc ggcggccctc gtcaaccacc aggacgcgct ttgccgccgt
    27841 cgcctgctcg acgatgaagg cctcgttcaa cggcaccagc cagcgcaggt cgacgattcg
    27901 cacgttccac ttgcgcttct tctcgatctc gcgcgcggcg cgcaggctca tcgggacccc
    27961 gtttccgtac gtgaagacca cgaggtcttc ggcaccctcg ttgtacacgc ggcccccgcc
    28021 cagcggcatc gcctggcccg gcgcggggta ctcgaactgc cattgcccgt cgccggactc
    28081 gtgcaggtcc ttggtcatgt acaacgcgat cggctcgagg aacacgcaca cgcgtccgtc
    28141 gaccttgcac agcgccgcga gcgtccgcag catcgtcgcg gcatcgtcgc cgcgcgaagg
    28201 gcagcccacc acgatgccgg ggatgtcgcg cagtgccgcg atcgaattgt cgttgtggaa
    28261 atgcccgccg aaccccttct ggtagccgag ggaggcgatc cggaccagca gcgggttgcg
    28321 gtattggtcg ttcgagaaaa actgcaggga cgccgcttcg ccgcggatct ggtcgcaggc
    28381 gttgtggaaa taggcgaggt actggatctc ggggatgggc agcatgccca tgttggcgta
    28441 gccctgcgcg agtccgagga tggtcgtctc gtccagcagc gtgttgaaca cccgcgcgcc
    28501 cttgaacgcc ttgtagagcc cgcgggtcag cgtgtacacg ccgcccttct gcgcgacatc
    28561 ctcgccgaat accagcgact cgcggtactt ggcgagcagg tcgtgcagcg cggcgttgat
    28621 ctggagcgcg aggtgccgtg gcggctggac ttccggcagc ttgtccttgc ctccgaaaat
    28681 ggcggcgcgc ctgtcgctgt aatccgcgcg cgcggcttcc gccgcaacgg cgtccggcgt
    28741 atacggcgcc agcggcctga ccacggcttc gagcgattcg agtttcgggc gaccgtaggc
    28801 cgcctcggcc gccgcgaagc agcgcttgcg aatcgcctcg tattcgtcca gcagcgcttg
    28861 ctgcgtgtac aaaccggatt ccagcgcgat cgatgccgaa cgcagcagcg gagcggtggc
    28921 ttccagcgcc atcagttcct cgaagctgcg ccactcgatc tcgaagtcgg tgccggcgtg
    28981 gcctgcgagc cgcgtggtcc tgaggtgcag gaaggtcggg cggcgcgtgc cgcggcaatg
    29041 ctccaccgcg cgttgcacct cggcatggcc ttccgcgaga tcgaggccgt cggcgaagaa
    29101 ataatcgaga tccttgcgat cgcggaaatt gttcgcgacc cagccactgg gcgtcttcac
    29161 cgaaatgccg atgccgttgt cctcgcatac gaacagcacc ggcgcgggca accgctggta
    29221 ggcggtgtag gccgccgcat tgaacgctac ctgcgcggtg gcgtggttgc tcgaggcgtc
    29281 gccgaacgag cagatcacga tcgaatcctt cggcaccggc agttcgtgcc ccatgcgcgg
    29341 tgcctgctcg atcgcgaccg cggtgccgaa cgccttcggg aggtgcgagg cgatggtgga
    29401 cgtctgcggc agcacccaca acgccttcga accccagacc ttgtggcggc cgccggacgc
    29461 cgggtcttcg ctgctggctg cgaagctgag cgcagagtcg accaccgggt cgacgtccgg
    29521 cactttccgg gaccgctcgg ccatgaacgc gccggatcgg tagtgcagga acgcgggatc
    29581 ggtatgccgg gtcagccgcg cgaccatcgc gttgccctcg tgcccgctcg acccgatcgt
    29641 gtagaagacc ttgttctgca cgcgcagcac gcgtgccatc aaatccaaat gccgcgaaat
    29701 caattgcgat tcgaacagct cgcgaaaatc gcgtgcggtg caggtgctgc ccggcaacac
    29761 cggcgcatcg tcgcgcggcg ccggcaaggc gcggccatgc cacgcgtgca cgtattccga
    29821 aaaattctgg tcgacgattt ccgcgcgatt gaagcccttg tggcgggccg ggacgggagt
    29881 cttgatggcg ttcatgtggt tgtggcagtt gaggtgcgtc gcgccagcca ttgctcgcgc
    29941 gtctgtcccc agatgtcgat ggggtctgcg ccgtacggcg ccggcagcgt gccctgcccg
    30001 cgcttcaccg aacccagtcg tcgcgcgacc gcctgcgacg ggaggttgtc gggcgcgatg
    30061 cagtggatga tctcgctcca acccaggtgc gcgaacgccc agtcggtcgc ggcgatggcg
    30121 gcctcggtcg cgtaaccgtt gccccatgct tcgcgggcaa gcgtccagcc gatctcggtg
    30181 cccggccagc cttctggacg cagtggcccg acgcgaccga cccattgtcg atccgccttg
    30241 cgcatgaccg agaaagcgcc gaacccgagc acgtgccagg agccgatcgc ggacagcaac
    30301 cggttccagg attcaaagcg cgtttgcacg ccgcctatgt ggcgcgtcgc ctgctcgtcg
    30361 ccccccagcg cagcccaagc ctcgaaatct tcacggcagg ggacccgcag gatcagccgc
    30421 gccgtttcca gcgtgggggc cagcgggggc atcgcaccgg gcattcgtgt catttggccg
    30481 gcggatggaa ggtgcactcc ggcgccgccg tcgcgatgtc gggaatgttc gggctgtagc
    30541 aggtgaattg cacgtccttc tcgccgccct tggtggtgcc gatggccggc accagttgca
    30601 ggatgccgcc ggtgacgccc gccctcggcg tgaggtacac gttgatcacc ccgccctcgc
    30661 tgacgatgat cgcgcggatt tcttcatcga ccagcttgtc gggcgcggcg acatgcgctt
    30721 gctcgttgct cgacggcgtg gtcccggcca gcatcgtgtt ggccacgctc gccttgacca
    30781 gcgccagccg caacgaactc aacgcgatgt gcacatggtc cgcgttgacc gcagccatgg
    30841 cgccgcccga ggccagcatt gcgccgactg cgaaacctgc aagccacttt ttgcttttca
    30901 tgttcaaact cctgcctctc tgcccaattc gaagcagctg tcgtgaaaag tcaggccacg
    30961 gatggccgtt ctggtgtccg atgaatctga aggcatccgg ttcatcattc agtgaaatga
    31021 aggcgcgatc agggatgatc gcaatcaaaa tgcgttgatg cgggtgagtt cgcggccgac
    31081 cacgagttgg tgcacggtct gggtgccctc gtaggtcacg accgattcca ggttgagggc
    31141 gtggcggatc gcgacgtgtt cggtggtgat gccggcgccg cccaggatgt cgcggcattc
    31201 gcgcgccacg tccagcgcca tccgcacgtt gttccacttc gccatcgaga cctgcgtggc
    31261 atgcaggcgg ccggcatcct tcaggcggcc cagccgcagc gccagcagtt gcgcggtggt
    31321 gatgcggcgc gcgatatcgg cgaggcgcaa ctggatcgcc tggttcgcgg ccagggggcg
    31381 gccgaacaac tggcgctcct gcgtgtagtc cagcacctcc ttgaggcacg cctgggcggc
    31441 cccgaccgga ccccagctga tgccgaagcg tgccgagtcg aggcagctca agggaccgcg
    31501 gaggcctttc acattgggaa gcatcgcgct ggccggcacg cgcacgtcgt cgaggaacag
    31561 cgcgctcgtc accgacgcgc gcaggctcat tttcttgtgg atttcctgcg cggcgaaacc
    31621 cctggtgtcc gtcggcacca ggaagccgcg gatgccgtct tcggttttcg cccacaccag
    31681 cgcgacgtgc gcgaggttgc cgttggtgat ccacatcttg gaaccgttga tcacccagtc
    31741 gccgccatcc tgcttcgcga cggtcttcat gttggccggg tcggaaccgc catgcggctc
    31801 ggtcaacccg aagcagccga tcacctcgcc cgccgccatc ttcggcaggt attcccgctt
    31861 ctgctcctcg ctgccgaagg cgaagatcgg gtacatcacg agcgacgact gcaccgacgc
    31921 gaaactgcgc aggcccgaat cgccgcgctc catttcctgg cagatcagcc cgtagctcac
    31981 ctggttcatc cccgcgccac cgtactcttc gggaatcgtt gcgccgagca ggccgagcga
    32041 cgcgatttcc gggatcaatt cggtcggaaa gcgggcctgg tcgaaacaat cgccgatgat
    32101 cggcagcacc ttgtcgtcga cgaagcgggc gacggagtcc tgcaccatcc gctcctcgtc
    32161 ggagagctcg gcacgaacgt cgtacaggtc gaggggattg agcggtgcgt ggggcatgtc
    32221 gattccggcg ggagacgcag ccggtcattc taatggggac gggggacggg tagcgggggc
    32281 gctcggtccg cgcgataccc gtttccggag acggtgctcc ctgtccaggc ggaccacgtt
    32341 gatctcaccg tccgtttttc gcccccccac tacccatctc ctgcgcccgg ctgttcgaca
    32401 ccccgtgcgg cacgtggccg gtcgcgacat gccgtcgggc cgactcgaca ttgatcggcg
    32461 agtcgtcgaa gaacaggtcc gcgccgaagg cctcgaggaa cggtcccttg tcgcgcccgc
    32521 cgaggaacag cgcttcgtcg atccggacgc cccactgccg cagggtcagg atcacgcgct
    32581 tgtgggcagg cgccgagcgc gccgtgacca gggcggtgcg gatcggcgaa ttctccgggg
    32641 ggaacgccgc ctgcagccgg tgcagggcag cgaggaagcc tcggaacgga ccgcccggca
    32701 gcggctcgtc ccagcgctcg gattcgttgc ggtggaaggc atcgatgccc tgctcctgcg
    32761 acacgcgctc ggagtcgtcg ctgaagataa ccgcgtcgcc gtcgaaggcg atacgcaact
    32821 gctcgggatg ggcgtcgtgc atctgcgagg gcaggatggt cgccgccgcg acgccggagt
    32881 gcagcgcgcg cgcgacgttc tccgcgttcg cggacaggaa caggtccacg gcgaatggcg
    32941 cgatgtattc gctggtggcc tcgcccgacg tgaacgcggc gcgggtgatc gcgagtccgt
    33001 aatgctggat cgaattgaag atgcgcaatc cggtgtcgcc cgaattgcgt gacagcagta
    33061 tcacctccac gtacgggacg tcgggctccg gtcggttcag cgccagcagt ttcttgacca
    33121 ccgggaacgc cacgcccggc ccgagcacgt cattctcgtg ggcgatctgg tagccgcgat
    33181 acgcctccag tccttcccgc tcgaacagcg catgcgattc ggtgaggtcg aacagcgcgc
    33241 gcgaggaaat agcgacgacc agcttgttgt cgcgggttgc cttctcgtcg tggggcggtg
    33301 gagttgccac atccgcagtg tatcgcgcga tcgtggcaga gacgctgacc cttgctcgcc
    33361 gccggcgcgg ctatcggcag cgcggtcccg acaattccag cctacgccca gagcggcctc
    33421 gagatttcca ccagccccca cgccacgata gcgctgatcg gcagggtgag gatccaggac
    33481 cacaccatcc gctcgaccac cgtccagcgc accgcattga agcgcttgga ggccccgacg
    33541 cccatgatcg ccgacgacac cacgtgggtc gtcgataccg gcagcccgaa taccgacgcg
    33601 gcgatgatga cggcggcagc gctggtctcc gcggcaaagc cgttgatcgg gtgcagcttc
    33661 accatcttgt ggccgagggt cttgatgatc cgccagccac cacccgcggt cccggcggcc
    33721 atcaccagcg cgcacagcac ctttacccac accggaatcg aaaactggcc acccacgtcg
    33781 gacacgcgca ggaagtgcag ccacgacggc aggtgatcga agttgtgcgc ggcggtggcg
    33841 ctcgcgagcg ccagggcgat gatgcccatc gacttctgcg catcgttcag cccatgcgat
    33901 atccccatcg ccgccgccga gaacagctgc gccttgccga agaagaaatt cacgaacggc
    33961 gtgcgcccga gccggcgcag gaatccggtg cggttgcaga accacgcgaa cagcgccagc
    34021 agcagtccca tcaccacgat ccccagcacg aagccgccga tcggggaaac caccatcggc
    34081 acgatcacct tgggaaacac cccgtcgccg cgccaccagg ccgagggatc gctcgcccag
    34141 atgatggcgc ggatgttgcc gccggaagcc gcgaaggccg cgccgcacaa gccgccgatc
    34201 agcgcgtgcg acgacgacga cggcagcccc agccaccagg tgatcaggtt ccagatcgtc
    34261 gcccccagca gggcgcagat cagcacctcg gcgccgacct gcaatacccc ggcgtcgatc
    34321 aaccccgatg cgatggtgat cgccaccgcc gaacccaaca gcgcgcccgc aaggttggtg
    34381 gttgccgcca gcagcaccgc ctgccctggc gacagcacct tggtcgcgac caccgtcgcg
    34441 atcgaattgg cggtgtcgtg gaaaccgttg atgtaggtga acaccaacgc gatcaccacc
    34501 acggcgatga ggatgccgag agtcatgtgg aagccggaag tcgggaatcg gaaagcgggg
    34561 atcgggttcg gga
//
LOCUS       DASSNH010000140         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_933157, whole genome shotgun sequence.
ACCESSION   DASSNH010000140 DASSNH010000000
VERSION     DASSNH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933157"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFK00_05185"
     CDS             complement(<1..891)
                     /locus_tag="VFK00_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7592381.1"
                     /translation="MFSKDLEFTIGQCYKEAREQRHEFMTVEHLLLALLANPTAAAVL
                     RGCGADLDKLAKDLRSIITETVPVLPNGDERDTQPTLGFQRVLQRAVYHVQSSGRKEV
                     TGANVLVAIFGEKDSHAVYFLHQQEVERLDVVNYVSHGIAKIGHDSSDAPATGEAGAD
                     VEPEGKDKDNALVEYATNLNERARQGRIDPLIGREEEIERTVQVLCRRRKNNPLYVGE
                     SGVGKTALAEGLALRIIEGKVPAVLEDATVWALDLGALVAGTKYRGDFEKRLKAVIAQ
                     LKKDPNAILFVDEIHTIIGAG"
     gene            complement(940..1272)
                     /gene="clpS"
                     /locus_tag="VFK00_05190"
     CDS             complement(940..1272)
                     /gene="clpS"
                     /locus_tag="VFK00_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET7592382.1"
                     /translation="MTPGKTNDNETEREQGVVVETSRPEAARPPLYQVVLLNDDYTPM
                     EFVVAVLETFFNMARERATQVMLHVHTRGKGVCGVFSREVAESKVTQVNEYSRIHQHP
                     LLCTMEKL"
     gene            1417..1911
                     /locus_tag="VFK00_05195"
     CDS             1417..1911
                     /locus_tag="VFK00_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7592383.1"
                     /translation="MDEFAATPEPAGIWCPHVTVACVVAEGDRFLMVEEDIHGELRFN
                     QPAGHLEEDEALQAAACRETLEETGWEIALDSFIGVQQWHSPVHKHHIVRFAFAGRPV
                     RHHPERALDRGITRALWMSYGEIADARARLRSPLVLTTLDDWLAGQRLPLGTLRSLLP
                     ARGA"
     gene            1914..3014
                     /gene="mnmA"
                     /locus_tag="VFK00_05200"
     CDS             1914..3014
                     /gene="mnmA"
                     /locus_tag="VFK00_05200"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7592384.1"
                     /translation="MRVVVGMSGGVDSSVAALLLQRDGHEVSGLFMQNWEDDDDPGSD
                     CRADADRKDALAVCAKLGIPFRDTNFAAEYRERVFAHFLDEYRAGRTPNPDVLCNREI
                     KFGSFLAAARAAGAEKIATGHYARVAHADGQWKLLRAADAGKDQTYFLHALDQEQLAA
                     TLFPLGGLRKVEVRRLADEASLPVAAKKDSTGICFIGERNFRSFLSNYIPAQPGELRT
                     PDGNVVGEHAGAMFYTPGQRSGLHIGGRAGGSGEAWYVVGTDVGVNVVYVDQGHDSTW
                     LQSRSISASGTHWIAGAAPSADFECVAMTRYRQPPQACRVTVEGDRCSVTFEGRQRAI
                     APGQSVVFYRGEECLGGAVIDATDAPAIGARP"
     gene            3011..>3573
                     /gene="hflD"
                     /locus_tag="VFK00_05205"
     CDS             3011..>3573
                     /gene="hflD"
                     /locus_tag="VFK00_05205"
                     /inference="COORDINATES: protein motif:HMM:NF001246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HET7592385.1"
                     /translation="MKETRVLALAGVFQAVSLVRDLARAGECDGEAMEASLASVFKLE
                     SDSAADVFGGNAGVRRGLRVLIEQVEGGDRDLPLFHMLVNVLKLERSLSRNAEVNRRL
                     GEALEGMQRQLQHFPVTHPTILARLADIYSAHLSNLKPRITVVGNPLYLQQPAQAQRV
                     RALLLAATRAAVLWHQLGGRRRHLLFRP"
BASE COUNT          578 a         1170 c         1198 g          627 t
ORIGIN      
        1 acccgcaccg atgatggtgt ggatttcgtc cacgaacagg atcgcgttgg gatccttctt
       61 gagctgggcg atcacggcct tcaggcgctt ctcgaaatcg ccgcggtact tggtgcccgc
      121 gaccagcgcg ccgaggtcga gggcccaaac ggtggcgtcc tccagcaccg cgggcacctt
      181 gccctcgatg atgcgcagcg ccaggccttc ggcgagcgcc gtcttgccca cgccggattc
      241 cccgacatac agcggattgt tcttgcgccg gcggcacaac acctggacgg tgcgctcgat
      301 ctcttcctcg cggccgatca gcgggtcgat gcggccctgg cgggcgcgct cgttgaggtt
      361 ggtcgcgtac tcgaccagcg cattgtcctt gtccttgccc tcgggctcga cgtcggcacc
      421 ggcctcgccc gtcgcggggg cgtccgaaga gtcgtggccg atcttggcga tgccgtgcga
      481 tacgtaattg acgacgtcga ggcgctcgac ctcctgctgg tgcaggaagt agacggcgtg
      541 tgaatccttt tccccgaaaa tcgccaccag cacgttggcg ccggtgactt ccttccgtcc
      601 ggaagactgc acatgataaa ccgcccgttg caacacccgc tgaaagccga gcgtcggctg
      661 ggtgtcgcgc tcgtcgccgt tcggcagcac cgggacggtt tccgtgatga tggagcgaag
      721 gtccttggcg agcttgtcca ggtcggcacc acagccgcgc aggaccgctg ccgcggtcgg
      781 gttggccagc aacgcgagca acaggtgctc caccgtcatg aattcgtgac gctgctcgcg
      841 ggcctccttg tagcactggc cgatggtgaa ttcgaggtcc ttgctgaaca tgcttctcgg
      901 tctccgggat ctctttatct aggcccgata tgggggcggt tacaactttt ccatcgtgca
      961 aagcaacggg tgctgatgga tgcgggagta ttcgttcacc tgcgttacct tgctttctgc
     1021 aacttcacgg ctgaacaccc cacaaacgcc cttgccgcgg gtgtgcacgt ggagcatcac
     1081 ctgggtcgcc ctttcacgcg ccatgttgaa aaacgtttcg agcaccgcca ccacgaactc
     1141 catcggggtg tagtcgtcgt tgagcagcac gacctggtag agcggcggcc ttgccgcctc
     1201 gggacgactg gtctcgacca ccaccccctg ctcgcgttcg gtttcgttgt cgttggtctt
     1261 gcctggagtc atcgaaaagg aagtcgtagg cctggtgtgc cggaatcgtg ccgcccgacc
     1321 cgagtatatg ccgtggccgc ccgcagggaa tggcagaacg tcacaccaga taatggcaaa
     1381 atggtgcaat ttccagtgcc acgcgacatg gagcgtatgg acgaattcgc cgcaaccccg
     1441 gagccggccg ggatctggtg cccgcacgtc acagtggcct gcgtggtcgc cgagggggac
     1501 cgtttcctga tggtcgagga agacatccac ggcgaactgc gtttcaacca gccggccggg
     1561 cacctcgagg aagacgaagc cctccaggcc gccgcgtgcc gggagaccct ggaggagacc
     1621 ggctgggaaa tcgcgctcga cagcttcatc ggggtgcagc agtggcacag tccggttcac
     1681 aagcaccaca tcgtgcggtt cgcctttgcc ggacggccgg tcaggcacca ccccgaacgc
     1741 gcactcgatc gcgggatcac gcgtgcgctg tggatgagct acggcgaaat cgcggacgcc
     1801 cgtgcgcggc tgcgcagccc gctggtgctg accaccctcg acgattggct ggccggccag
     1861 cgcctgccgc tgggcaccct gcgctcgctg ctgcccgcgc gcggagcgtg acgatgcgcg
     1921 tggtggtcgg catgtccggc ggggtcgatt catcggtggc tgcgctgctc ctgcagcgcg
     1981 acgggcacga agtatccggc ctgttcatgc agaactggga ggacgacgat gatccgggat
     2041 ccgattgccg ggccgatgcc gatcgcaagg acgcgctggc cgtgtgtgcg aagctcggca
     2101 ttccgttccg ggacaccaat ttcgccgcgg aataccgcga gcgcgtgttc gcgcatttcc
     2161 tggacgaata ccgcgcaggc aggacgccca atccggacgt gctgtgcaac cgcgaaatca
     2221 agttcggcag tttcctcgcc gccgcccgcg cagccggtgc ggaaaagatc gcgaccggcc
     2281 attacgcccg cgttgcgcat gcggatggcc aatggaagct gttgcgcgcg gccgatgccg
     2341 gcaaggacca gacctatttc ctgcatgcac tggaccagga gcagctcgcc gcgacgttgt
     2401 tccccttggg tggccttcgg aaagtcgagg tgcggcggtt ggccgacgaa gcgagcttgc
     2461 cggtggccgc gaaaaaggac tccaccggta tctgcttcat cggcgagcgg aatttccgga
     2521 gcttcctctc gaattacatc ccggcgcagc cgggcgaact gcgcacgccg gacggcaacg
     2581 tcgtcggcga acacgccggc gcgatgttct atacccccgg gcaacgctcc ggcctgcaca
     2641 tcggcggacg cgcgggcggc agcggcgagg cctggtacgt ggtcggcacc gacgtcgggg
     2701 tgaacgtcgt gtacgtggac cagggccatg acagcacctg gctgcagtcg cgttcgatct
     2761 ccgcgtcggg tacgcactgg attgccggtg ctgcgccatc tgccgatttc gaatgcgtgg
     2821 cgatgacgcg ctaccgccag cctccccagg cgtgccgcgt gacggtcgag ggtgaccggt
     2881 gcagcgtcac gttcgaaggg cggcaacgcg ccatcgcgcc gggccagtcg gtggtgttct
     2941 accgcggcga ggaatgcctg ggcggcgccg tcatcgacgc aacggatgcg ccggcgattg
     3001 gggcgcggcc atgaaggaga cccgcgtgct cgcgctggcg ggggtgttcc aggccgtgtc
     3061 cctggtccgc gacctcgcgc gcgccggcga gtgcgacggc gaggcgatgg aagcgagcct
     3121 cgcgagcgta ttcaagctgg aatccgacag cgcggcagac gtattcggcg ggaacgcagg
     3181 agtccgccgc gggctgcggg tcctgatcga gcaggtcgag ggcggcgacc gcgacctgcc
     3241 gctgttccac atgctggtca acgtcctcaa actggagcgc agcctgtccc gcaacgcgga
     3301 ggtcaaccgg cgcctcggcg aagcgctgga aggcatgcag cgccagttgc agcatttccc
     3361 ggtgacgcat cccacgatcc tggcgcgtct cgccgacatc tacagcgccc acctgtccaa
     3421 tttgaagccg cgcatcacgg tggtcggcaa cccgttgtac ctgcagcagc cggcgcaggc
     3481 ccagcgcgtg cgggcgctgc tgctggcagc cacacgcgcc gccgtccttt ggcaccagct
     3541 cggtggccgc cgccggcacc tgctgttccg ccc
//
LOCUS       DASSNH010000141        10952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1519880, whole genome shotgun sequence.
ACCESSION   DASSNH010000141 DASSNH010000000
VERSION     DASSNH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519880"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5777
                     /locus_tag="VFK00_05210"
     CDS             <1..5777
                     /locus_tag="VFK00_05210"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592386.1"
                     /translation="SAFAASKSSTAVGDSSTAEGNNASAFGYSAYAGSPSSTAVGTGA
                     NAMGNSSTALGSGADTAGFANSVALGAGSVVDREDSVSVGAPGAERQVTNLAAGTADT
                     DAVNLAQLNEATNDGEAAATSHYFKANGPADGSDDAVASGAHATASGPLATASGDYSA
                     AFGASAAATAVNGIAIGHGAIASAGEGTAIGKYAAATGTSSNAVGYDAQATGLFSNAF
                     GGQTLASGFESLAIGQGAGATGRQATSVGGFAGGTFSTAVGAGAFATADQATALGKYA
                     AARGTASSALGKYATATGTAATAVGAYSFAPSYAATAVGVQARASGLYASAEGFGAYA
                     SGYGGTAIGANAAASGDSSTALGSTSLASGAYGTAVGRFALAEGYSSVALGDTANALG
                     DDSTAVGRYATAAGSNDVALGAGAMTVAGDSSMGNNIAIGTYASAGSAYGGGATAVGN
                     QASAYGAYAAAFGSHAFAGLPGALAGGYQSSATGEWSVALGERADASDIGAVAIGGGA
                     IASGSTSVAIGGQFNTFVAFVHTKALGSHSVAVGPGALSQGDFSIALGDESTALGDFS
                     TTLGGGSSAQGVQSSAVGMNALAQGDASTAVGGNAAGYGVLSSAFGFRAVAAGDVSLG
                     LGAYAVAVGTGSIAAGLQAYVGADGYNAVALGAHAYASAQGAQALGAMSHASGAMSVA
                     AGYSAFADGESSTAVGDTAGAMGDYSTAVGANAYASGSFASAFGDDAEASGNSSTALG
                     ASASAVGDDATAVGAGAYSGANDIASGAHSHAMGNASSAFGTYASASNSATTALGSHA
                     VASGLGATTVGAYASATGKYGTALGVSSNAYGVRALAAGFRANADTFGTAVGTDANAG
                     SYGSAAVGYAAEALGVQATAFGMHAKAYADRSVADGYGAYVASDAASAVAIGSYAAAM
                     GYSSAALGAHSTASGYASTAVGSNASAAGDSSVALGSTAMASGAYGTAIGPFAVATGE
                     SSTALGDTASAVGDDAAAVGASAYATVNGTAVGAHSTANGYGGNASAFGIYASATAPV
                     AAAFGDHSVASALGATAAGAYSTATGKYGTALGMSAHANGINSLAAGFRANAGNFGTA
                     AGTHAYAGGYGSSAFGFAAQAVAEQSTALGMDASAYAFRSIAEGYRAHVDASATRGIA
                     IGSYSSSAGYSSAALGAYAVAAGDSSLALGQTTVAGGGYSSAVGPFAVALGESSSAFG
                     DTANAFGDYSSAFGAYSTALGMFATAGGFDSLAGAKWSVAYGPGSTATGQSAVAIGGQ
                     MTVIDGTTWEPETWSTEATGQESTAIGTAAHATEYATTAVGSTAEASAQGAVALGTAS
                     TARGEESIALGTMARAYDTLGIAIGSSAKAQTPRSVALGEMSVTGGDGAVAIGGISAA
                     GGDQSVALGWNAITFPGATNTALGASAEVAPGAQNAVALGNNSYADRANTVSVGTPAR
                     VSDGQFPYEIAAMDRQITHVAAGTEDTDAVNVAQLHEATEAGSATAAYFTANGAGDGS
                     DDAVAIGTNAVAAGPNASASGDAGLALGMGSVASGLGASALGSGTSAGGINATAAGAG
                     ASAGGNNASALGAGSLASGMNSAAFGAGAIASGSSAAAFGAGAQATATNSVAIGAGSM
                     ATFMNTVSVGSVGNYRRVVNVADPIDGHDAVTLGYLQNNYSDNDAISNLSHEIDALNK
                     QMASLKGEPDATDAGTATAETASTMIASSPNAKRSTANQDAVADSPAAMSRSAAAAPD
                     SEGAAAQGPAISESAVRGTVAATAESKQPVQAASDSAGTASTADATQAVAQTTEVAST
                     ANAYTDQQTREALQSAQTYTDASTANAISQAKAYTNQALAGYVTSDTFDEFQKQTADR
                     FNATDERINRTSAMSTAMVQMAASASGIDTDNRVGVGYGNTHGRSALAVGYQRAVGKK
                     ATITIGGSVSGSESSVGAGVGFGW"
     gene            5908..8187
                     /locus_tag="VFK00_05215"
     CDS             5908..8187
                     /locus_tag="VFK00_05215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HET7592387.1"
                     /translation="MSTHRQHPRFRALAFAIAGTLGISSVALGAPQRVQFTAITPATR
                     LAHGDVVHGAMPLSQSIRVTLVLKLRNLSQLEAFNARPHASMSQAQLAASHLPTKSQA
                     EAVAGFLKRSGFRNVAISENRMLVSGYADAGKVAQAFRTSFARVRTHDGRAAFANVSN
                     VNIPAALQDTVQAVLGLQTVHKAHVLARAGGQATTGGIGGHGPLEFASIYGADGLQPA
                     TNVDVAVWGWGSMAQTLADLDTFTDANGLPRVNAQVVCTDYDGHDAGGFSTTDPTCNN
                     FEQGTVEWDLDSQDIVGMSGGVRSLTFYAAYGGFNDSMANALNEIVTPTQGEPLASVI
                     NASFGECERYQDVNQGGDGSAQAYDALFQIAQAQGQTFAVATGDSGADECGDGQSDSA
                     SFPASSPYVIAVAGTTLRASSTTWERENEWIGSGGSPSSFEQAPAWQAPLTYGPHAGM
                     RGPDVAFDANPSSGAIIYNAGGMLQVGGTSLAAPLFAGAWARLLANGAVDPMTPAGQQ
                     LYAMPASYFHDIRSGNNRGYVTRRGWDWASGRGSLDLSKVPLPNVFVENYDDGTDTGQ
                     WLVSNSTPRVIESSGGNPGAFLEQGGFSTHIPTWGSASPRYQPGYNDTWKVDSVFTGD
                     WADAGVTTFSIDMDIIQAGGWGPDRAVTLELLQMDDSGFGVNYEATYTIPLGSEPPAG
                     WQNYAFPVNADSATIPPGWVFTRGDGTPGTDAEWSQFLKRIDLTSVGYYKPGYAYIGL
                     GSWTLGIDNVTLRTVPPGA"
     gene            8373..9320
                     /locus_tag="VFK00_05220"
     CDS             8373..9320
                     /locus_tag="VFK00_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7592388.1"
                     /translation="MSNLLPLPEPERCEAEAARRPEIESWPARTADLGGGLSVVRALP
                     LRQHRLVGPWCFFDSYGPLSFAGRKAMDVAPHPHIGLQTVSWLFSGEVLHNDSLGFEA
                     MARPGELNLMTAGRGIAHAEETPPRNSGKLHGVQLWVALPEPERGGEPAFDHYRALPV
                     LEPGGGQVQVFMGELAGVRSAGRAFSPMVGAEIRTGGSATLRVPLEASWEHALVVIEG
                     ELRVDGVRLAPGALHYLGTHRDGLELESRAPARGILIGGAPFGEAIMIWWNFVARTAE
                     EISEARDDWQAGRRFGEVSAYRGERLAAPELVGRPRLSR"
     gene            complement(9389..9465)
                     /locus_tag="VFK00_05225"
     tRNA            complement(9389..9465)
                     /locus_tag="VFK00_05225"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9429..9431),aa:Met,seq:cat)
     gene            9655..>10952
                     /locus_tag="VFK00_05230"
     CDS             9655..>10952
                     /locus_tag="VFK00_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7592389.1"
                     /translation="MRRFQLAEPLVMLATVVQWLVLSMFTGAVIGVGCTIFLRVLFAT
                     AGRAYAAPLWLQMTLLPIGGIANGLLLHYGYRLSRSGFKDNPIVAVNEQQGKMPFRTL
                     WIKPLAALVTLGCGGSAGKEGPCSHIGASLAAGIGRVLHLNPEMRKRLLACGVSAGFA
                     SVFGTPIAGAIYGVEVLAIGRIRHDFLFPGIVAGVTAFEVSRWLGVPYPVYHIAFVGD
                     FTELLFLKTVVIGILCGAVAWIFVELIHLMRHAAGWLRARYALWPPLLPLLGGVLLAI
                     LILAIPTRYLGLSLPLMDDALHGAPMPYLGFFWKALLVAITLGSGFYGGIVTPQFVIG
                     AVTGGAFAHLLGMKAALGAAVGLVAVVASASNAPIAAVLMGVELFGSDSTLYVAGACV
                     AAYLIIGHRSVYPEQQVAYSKSSWIFARTDLPVGQEKVRLS"
BASE COUNT         1761 a         3658 c         3872 g         1661 t
ORIGIN      
        1 ggagtgcctt tgcggccagc aagtccagta cggcggtggg tgactcgagc accgcggaag
       61 gcaacaacgc gagcgccttc ggctacagcg cgtatgccgg cagcccgtcc agcacggcgg
      121 tcggcacggg cgcgaacgcg atgggcaaca gcagcacggc gctcggcagc ggcgccgaca
      181 ccgccggctt cgcgaacagc gtggccttgg gtgcgggttc ggtcgtggat cgcgaggaca
      241 gcgtctcggt gggcgcgccc ggtgcggaac gccaggtcac caacctggcg gcgggcaccg
      301 cagacaccga tgcggtgaac ctggcgcagt tgaacgaggc caccaacgac ggcgaagccg
      361 cggcgacctc gcactacttc aaggccaacg gcccggccga cggttcggac gatgcggtgg
      421 cgagcggcgc gcatgcgacg gcgtccgggc ccctggcgac cgcctccggc gactacagcg
      481 cggccttcgg cgccagcgcg gcggcgaccg ccgtcaacgg catcgcgatc ggccacgggg
      541 cgatcgcgag tgccggcgaa ggcaccgcga tcggcaagta tgcggccgcc accggcacat
      601 ccagcaacgc tgtcggctac gacgcgcagg cgaccggcct gttctcgaat gctttcgggg
      661 gccagacgct tgcgtccgga ttcgaatcgc tggcaatcgg ccaaggcgcg ggagcgaccg
      721 ggcgccaagc cacgtcggtg ggaggctttg ccggtggcac gttcagtacc gccgtgggtg
      781 ccggggcgtt cgccacggcg gaccaggcca ccgcgctggg caaatacgcg gcagcccgcg
      841 gcaccgcgag cagcgcgctc ggcaaatacg cgaccgcaac cgggacggca gcgacggcag
      901 taggcgccta ttcattcgca cccagttacg cggccactgc ggtcggcgta caggcgcggg
      961 cgagcgggct ctacgccagc gccgaaggct tcggggcgta cgcgagcggc tacggcggaa
     1021 ccgccatcgg ggccaatgcc gccgcctcgg gcgactcgtc cacggcattg ggcagcacaa
     1081 gtctggccag cggcgcctac ggaaccgccg ttggacggtt cgcgctggcg gaggggtact
     1141 cctcggtggc attgggcgat acggccaatg cgctgggtga cgactcgacc gcggtgggcc
     1201 gttacgcgac cgcggcagga tcgaacgatg ttgcgttggg cgcgggcgcg atgaccgtcg
     1261 ccggcgactc ttcgatgggc aacaacatcg ccatcggaac gtatgcgtcc gcgggcagtg
     1321 cgtacggtgg cggcgccacc gctgtcggca accaggccag cgcatacggc gcctacgccg
     1381 cggcattcgg ttcccacgcg ttcgccggac tgccgggtgc gctggctggc ggataccaaa
     1441 gttccgcgac cggcgaatgg agcgtggcgc tcggcgaaag agccgatgcg tccgacatcg
     1501 gcgcggtcgc gatcggtggc ggtgccatcg ccagtggcag caccagcgtg gcgatcggcg
     1561 ggcagttcaa tactttcgtc gcgttcgtgc ataccaaggc cctgggttcg cactcggtgg
     1621 cggtgggtcc gggtgcgttg agccaaggcg acttttcgat tgcactgggc gacgagtcca
     1681 cggcactcgg cgacttcagt acgaccctgg gtggcggctc cagcgcgcag ggcgtgcaaa
     1741 gttccgcggt gggcatgaat gccctggcgc agggtgatgc gagcacggcc gttggtggca
     1801 acgccgcggg atacggcgtg ctgagcagcg cattcggttt tcgcgcggtc gcagcgggcg
     1861 acgtttcgct gggcctcggc gcctatgccg tggcggtcgg tacgggttcc attgccgcag
     1921 ggctgcaggc gtatgtcggc gcggatggct acaacgccgt ggcgctgggc gcccatgcct
     1981 acgcgtcggc ccagggcgcg caggcactcg gtgcgatgtc gcatgcgtcc ggcgccatga
     2041 gcgttgcggc gggttactcc gcgttcgccg acggtgaaag ttccacggcc gtcggcgata
     2101 cggcgggcgc gatgggcgac tacagcaccg cggtgggcgc gaatgcttac gccagcggat
     2161 cgttcgcttc cgcgttcggc gacgacgccg aagcttccgg gaactcgtcc acggcgctgg
     2221 gcgcgtcggc gagcgccgtc ggtgacgatg ccaccgcagt cggcgcgggt gcgtactcgg
     2281 gtgccaatga catcgcaagc ggcgcacaca gccatgcgat gggcaacgcg tcgtcggcgt
     2341 tcggcaccta tgcgagcgcg agcaattccg ccacgaccgc gctcggcagc catgccgttg
     2401 cgtcgggtct tggcgctacc acggtaggcg cgtacgccag tgcgaccggc aaatacggta
     2461 cggcgctcgg ggtgtcctcg aacgcatacg gcgtgcgggc gcttgcggcc ggattccggg
     2521 cgaacgccga cacgttcggt accgcggtcg ggaccgatgc caacgccggc agctatggca
     2581 gtgcggcggt cgggtatgcc gcggaggcgc tgggtgtaca ggcgacggcg ttcggcatgc
     2641 atgcaaaggc ttacgcggat cggtcggtcg ccgatggcta cggcgcatac gtcgccagcg
     2701 acgcggccag tgccgtcgca atcggcagct atgcggccgc gatgggttat tccagcgccg
     2761 ccttgggcgc ccactcgacc gcgtccggat atgcgagcac ggcagtcggc agcaatgcga
     2821 gtgcggcggg cgattcctcg gtggcgctcg gcagcacggc gatggcgagc ggggcctacg
     2881 gcacggcgat cggtccgttc gcggtggcaa ccggtgagtc gtccaccgcg ctgggcgaca
     2941 cggccagcgc cgtgggagac gacgctgcgg cggtgggcgc cagtgcctac gccacggtca
     3001 acgggactgc tgtcggtgcg cacagcaccg cgaacggcta cggcggcaat gcttccgcgt
     3061 tcgggatcta tgccagcgcg acggctccgg tggccgccgc gttcggggac cacagcgttg
     3121 cctccgccct cggcgcaacg gcggcgggcg cctattccac ggcgaccgga aaatacggca
     3181 ccgcgctcgg catgtcggcg cacgccaacg gcatcaattc cctcgcggcg gggttcaggg
     3241 ccaacgcggg gaacttcggt acggccgcgg ggacccacgc gtacgcgggc ggctatggca
     3301 gttccgcctt cggctttgcc gcgcaggcag tggccgagca gtcgaccgcc ctcggcatgg
     3361 acgcaagcgc atacgccttt cgttccatcg cggaaggcta tcgcgcccac gtcgatgcct
     3421 cggcgacccg aggcattgcc atcggcagct actcgagcag cgccggctac tccagcgcgg
     3481 cgctgggcgc gtacgcggtt gcggcgggag actcctcgct ggcactcgga caaaccacgg
     3541 tcgccggcgg gggatacagt tccgcggtcg gcccgtttgc ggtggccctg ggcgaatcct
     3601 cgagtgcctt cggcgacacc gccaacgctt tcggcgacta cagcagcgcg ttcggcgcct
     3661 actcgactgc actgggcatg ttcgcgacgg ccggcggctt cgacagcctc gcgggcgcga
     3721 agtggagcgt cgcgtacggc cccggcagta ccgccacggg acaaagcgcg gtcgccatcg
     3781 gcggccagat gaccgtcatc gatggcacga cgtgggagcc cgaaacctgg tccaccgagg
     3841 cgacgggaca ggaaagcacc gcgatcggca ccgccgcgca cgccaccgag tacgccacga
     3901 ccgccgtggg ttccaccgcg gaagcatcgg cgcaaggcgc ggtcgcgctg ggcacggcca
     3961 gtaccgcgcg cggcgaggag agcatcgcgc tcggcaccat ggcgcgggca tacgacacgc
     4021 tcggcatcgc gatcggttcc agcgccaagg cgcaaacccc gcgcagcgtg gccctgggcg
     4081 aaatgtcggt caccggcggc gacggtgcgg tcgcgatcgg cggcatttcc gcggccggag
     4141 gcgaccagag cgtggcgctc ggctggaatg cgatcacctt tcccggtgcc accaataccg
     4201 cgctcggcgc cagcgcggaa gtcgcgccgg gcgcgcagaa cgcggtggcg ctgggcaata
     4261 actcgtacgc ggatcgtgcc aacacggtat cggtcgggac gcctgcgcgg gtttcggacg
     4321 ggcagttccc gtacgagatc gcggcgatgg atcgacagat cacccacgtg gccgcaggca
     4381 ccgaggacac cgatgcggtg aacgtggcgc aattgcacga ggccacggaa gccggcagcg
     4441 ccaccgcggc ctacttcacc gccaacggtg ccggcgacgg cagcgacgat gcggtggcga
     4501 tcggcaccaa tgcggtagcg gcaggtccga acgcttcggc ttccggcgac gccggccttg
     4561 cgctcggcat gggcagcgtg gcgagcgggc tcggtgcttc ggcgctcggc agcggcacta
     4621 gcgcgggcgg catcaatgcg accgcggccg gcgcaggcgc ctccgcaggc ggaaacaatg
     4681 cgtcggcgtt gggcgcgggt tcactggcga gcgggatgaa ctcggctgcg ttcggcgccg
     4741 gagccatcgc cagcggcagc agcgccgctg cgttcggcgc gggcgcgcag gccacagcaa
     4801 ccaacagcgt ggcgatcggc gcgggctcga tggcgacgtt catgaacacg gtgtcggtcg
     4861 gctcggtggg caactaccgg cgcgtggtca acgtggccga tccgatcgat ggccacgatg
     4921 cggtgacgct cgggtatctg cagaacaact attcggacaa cgacgcgatc tcgaacctgt
     4981 cgcacgagat cgatgcgctg aacaagcaga tggcttcgct gaagggggag ccggacgcga
     5041 ccgatgcggg tacggcaacg gcggaaacgg ccagcaccat gattgcatcc tcgcccaatg
     5101 cgaagcggtc caccgccaac caggacgccg tcgcggacag tccggccgcc atgtcgcgct
     5161 ccgcagcggc cgcgccggat tccgagggcg cggcagcgca aggtccggcg attagcgaat
     5221 cggccgtacg cggcaccgtc gcggccaccg cggaaagcaa acagccggtc caagcggctt
     5281 ccgacagcgc aggcaccgca tccacggccg acgcaacgca ggcagtcgcg cagaccacgg
     5341 aggttgccag tacggccaat gcgtataccg atcagcagac gcgggaagca ttgcagtcgg
     5401 cgcaaacgta caccgatgca tccacggcga acgcgatcag ccaggcgaag gcctacacca
     5461 accaggcact cgccggctac gtcacgagcg acacgttcga cgagttccag aagcagacgg
     5521 ccgatcgctt caacgccacc gacgagcgca tcaaccggac ctcggccatg agcaccgcga
     5581 tggtccagat ggcggcgagc gcttccggca tcgatacgga caaccgagtc ggggtcggct
     5641 acggcaacac ccacggccgg tcggccctcg cggtcggcta ccagcgggcc gttggcaaga
     5701 aagccacgat cacgatcggc ggttcggtca gcgggagcga aagctccgtc ggcgccggcg
     5761 tcgggtttgg ctggtaagcc gtagtcgcag acgacgaagc aagtgaagtt gccgggcgcc
     5821 aggctgcgcc cggcaacggc gaaattcgca cgtccgcgtc ggcgatcaac gccaacgcgc
     5881 cattccacct gttgcagagg gatccttatg tccacccatc gtcagcatcc gcgtttccgg
     5941 gctctcgcgt tcgccattgc aggcacgctg ggaatttcaa gcgttgcgct gggcgcgccc
     6001 cagcgtgtcc agttcacggc aatcacccct gcgacacgat tggcccacgg cgatgtcgtg
     6061 cacggcgcga tgccgctgtc acaatccatc cgcgtcacgc ttgtgctgaa gttgcgcaac
     6121 ctgtcgcaac tcgaggcttt caacgctcgc ccgcatgcat ccatgagcca ggcgcaactg
     6181 gcggcgagcc atttgcccac gaaatcacag gcggaggccg tggccggttt cctgaagcgc
     6241 tcgggtttcc gtaatgtcgc gatttcggaa aaccggatgc tggtcagcgg atatgccgac
     6301 gccggcaagg ttgcgcaggc attccgcacg tctttcgcgc gcgtgcgcac ccatgatggc
     6361 cgcgcagcgt tcgccaatgt cagcaacgta aacatcccgg ccgcgttgca ggacaccgtg
     6421 caggcggtgc tcggcctcca gaccgtgcac aaggcgcacg tcctcgcgcg cgcgggcggg
     6481 caggccacca ccggcgggat cggcggacac ggcccgctcg agttcgcctc gatctacggg
     6541 gcggacggcc tgcaacccgc cacgaacgtc gatgtcgctg tgtggggctg gggcagcatg
     6601 gcgcaaacgc tcgcggatct cgacaccttc accgatgcca acggcctgcc gcgcgtcaac
     6661 gcccaggtcg tttgcaccga ctacgacgga cacgatgccg gcggattctc gaccaccgat
     6721 ccgacctgca acaacttcga gcaaggcacc gtggagtggg atctcgacag ccaggacatc
     6781 gtcggcatgt ccggcggcgt gaggagcctg acgttctatg cggcctatgg tggcttcaat
     6841 gattccatgg ccaacgcgct gaacgaaatc gtgacgccca cccagggcga gccgctggcg
     6901 tcggtgatca acgcatcgtt cggcgagtgc gagcgctacc aggacgtcaa ccagggcgga
     6961 gacggttccg cgcaggcgta cgacgcgctg ttccagattg cgcaggccca ggggcagacg
     7021 ttcgcggtgg ccaccggcga ttcgggcgcc gacgaatgcg gtgacggcca atccgactcc
     7081 gccagcttcc ccgcgagttc tccctacgtc atcgcggtgg cggggaccac gttgagggcg
     7141 tcgagcacga cctgggaaag ggaaaacgag tggatcggtt ccggcggcag cccgagttca
     7201 ttcgagcagg ccccagcatg gcaggcccca ctgacctacg gtccccacgc gggcatgcgc
     7261 ggaccggacg tggccttcga cgccaatccg agttccggcg ccatcatcta caacgccggc
     7321 ggcatgctgc aggtcggcgg caccagtctc gccgccccct tgttcgcggg cgcctgggcc
     7381 aggctgctcg cgaacggcgc ggtcgatccg atgacgccgg ccggacagca gctctacgcg
     7441 atgccggcgt cgtacttcca cgacatccgg agcggcaaca atcgcggtta tgtcacccgg
     7501 cgcggctggg actgggcttc cggccggggc agcctggacc tcagcaaggt gccgctgccg
     7561 aacgtcttcg tcgagaacta cgacgatggc accgacactg gtcaatggct cgtcagcaac
     7621 agcaccccgc gggtgatcga atcgtccggc ggcaaccccg gcgcgttcct cgagcagggc
     7681 ggcttttcca cccatattcc gacgtggggg tcggcatcgc cgcgctacca gcccggctac
     7741 aacgacacct ggaaggtcga cagtgtcttc accggcgact gggccgatgc cggggtcacc
     7801 acgttcagca tcgacatgga catcatccag gcaggcggct ggggtccgga ccgcgcggtc
     7861 acgctcgagt tgctgcaaat ggacgacagc gggttcggcg tcaactacga ggcgacatac
     7921 acgattccgc tgggttcgga accgcccgcg ggctggcaga actacgcctt tccggtgaac
     7981 gccgattccg cgacgattcc gccgggctgg gtgttcacgc gcggcgacgg cacgcccggc
     8041 accgacgcgg aatggtcgca attcctcaag cgcatcgacc tcacatccgt cggctactac
     8101 aaacccgggt acgcctacat cggcctcgga agctggacgc tcggcatcga caacgtcacg
     8161 cttcggacgg tgcctcctgg cgcttgaacg ggacgcccgg gacggcgcat cgagaagggc
     8221 cgccccgggc ggcccttctg ccgtgcgggg tggtgaggca actgatggtg tgcttcagcg
     8281 gcgctgtcgt gcgcgcgcgc cataatggag cggccggcgt cgagatcgtc cccggacgca
     8341 cggctttttc tgcaacggat gggtgcagcg ccatgagcaa cctgctgcca ttgccggaac
     8401 ccgagcgctg cgaagcggaa gccgcacgac gtcccgagat cgagagctgg ccggcgcgca
     8461 cggcggatct cggcggcggc ctctccgtgg tgcgggcgct gccgttgcgc cagcaccggc
     8521 tggtcgggcc gtggtgcttc ttcgattcct acgggcccct gtcgtttgcc ggccgcaagg
     8581 ccatggatgt cgcgccgcat ccgcacatcg gcctgcaaac ggtgtcgtgg ttgttctcgg
     8641 gcgaggtgct gcacaacgac agcctcggct tcgaggccat ggcccggccg ggcgagctga
     8701 acctcatgac cgcagggcgc ggcatcgcgc atgcggaaga aacgcctccg cggaattccg
     8761 gcaagctgca cggcgtgcaa ttgtgggtgg cgctgccgga acccgagcgc gggggcgaac
     8821 ccgcattcga tcattatcgt gccttgccgg tgctggagcc gggcggcggg caggtccagg
     8881 tgttcatggg cgaactcgcc ggcgttcggt ccgccggccg ggcgttctca cccatggtgg
     8941 gcgcggaaat ccgcaccggc ggcagcgcca cgctgcgcgt gccactcgaa gcctcctggg
     9001 agcacgcctt ggtcgtcatc gaaggcgagc tacgggtgga tggcgtgcgg ctcgccccgg
     9061 gcgcattgca ctacctcggc acccatcgcg acggtctgga actcgagagc cgcgcccccg
     9121 cgcgtggcat cctgatcggc ggcgcgccgt tcggggaagc catcatgatc tggtggaatt
     9181 tcgtcgcgcg caccgcggag gagatcagcg aagcgcgaga cgattggcag gccgggcgcc
     9241 gcttcggcga ggtcagcgcc taccggggag aacggcttgc agcacctgaa ctcgtcgggc
     9301 ggccgcgcct gagccggtga cagacgcggc ggcgcgcttc gaacggcaaa aaagcccatg
     9361 gcggcaccat gggctcgtgc gttgcagatg gtggctaggg gcgggatcga accgccgacc
     9421 tcagcattat gagtgccgcg ctctgaccgg ctgagctacc tagccaagaa ggcaattgta
     9481 ccggcgatga ctccggccca cgcagccgtg cgtcgaccgc cgagcgcgcc tcatcgccgc
     9541 gcaaagagcc gatcagtcta gcgaatgcgc aatggctgtc aaccgcccaa cggggcggga
     9601 gcttgtgacc gatatactcc cggtttgctc atcacgggga tcgtgagcca gtccatgcga
     9661 cgcttccagc tagccgaacc gctggtgatg ctggcgaccg tcgtccagtg gctggtcctc
     9721 tcgatgttca ccggcgccgt gatcggcgtc ggctgcacca tcttcctgcg cgtcctgttc
     9781 gccaccgccg gccgcgcgta cgcggcgccc ttgtggctgc agatgacgct gctgccgatc
     9841 ggcggcatcg ccaacggcct gctgttgcac tacggctacc ggctgagccg cagcgggttc
     9901 aaggacaacc cgatcgtcgc ggtcaacgag caacagggca agatgccgtt ccgcacgctg
     9961 tggatcaagc cgttggcagc cctggtcacc ctgggttgcg gcggctcggc cggcaaggaa
    10021 ggaccgtgct cgcacatcgg cgcgagtctc gcggccggca tcggtcgggt gctgcacctc
    10081 aatccggaga tgcgcaagcg cctgctggcg tgcggggtga gcgcaggctt cgcgagcgtg
    10141 ttcggcacgc caatcgccgg cgcgatctac ggcgtcgaag tgctggctat cgggcgtatc
    10201 cgccacgact tcctgttccc gggcatcgtc gccggcgtga ccgcgttcga ggtgtcgcgc
    10261 tggctcggcg tgccgtatcc cgtttaccac attgccttcg tcggggactt taccgaactg
    10321 ctgttcctca agacggtggt gatcggcatc ctgtgtggcg cggtcgcgtg gattttcgtg
    10381 gagctcatcc atttgatgcg gcacgccgcc ggctggctgc gcgcacgcta cgccctgtgg
    10441 ccgccgttgc tgccgttgct cggcggcgtg ctgctggcga tcctgatcct ggcgatcccg
    10501 acgcggtatc tcggcctgag cctgccgctg atggacgacg cgctgcatgg cgcgccgatg
    10561 ccgtacctcg gctttttctg gaaggcgctg ctggtcgcga tcacgctggg atcgggcttc
    10621 tacggcggca tcgtgacccc gcagttcgtg attggtgccg tcaccggcgg cgcgttcgcg
    10681 cacctgctgg gcatgaaggc ggcgctgggt gcggcggtcg ggctggtcgc ggtagtggct
    10741 tcggcttcga acgcgccgat cgcggcggtg ctgatgggcg tggaactgtt cggctccgac
    10801 tcgacgctct acgtcgcggg cgcctgcgtc gccgcctacc tcatcatcgg ccaccgcagc
    10861 gtgtatcccg agcagcaggt cgcctattcc aaatcgtcgt ggatcttcgc ccgcaccgac
    10921 ctgccggtgg ggcaggaaaa ggtgcgcctg tc
//
LOCUS       DASSNH010000142        10145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1641035, whole genome shotgun sequence.
ACCESSION   DASSNH010000142 DASSNH010000000
VERSION     DASSNH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641035"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFK00_05235"
     CDS             <1..849
                     /locus_tag="VFK00_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-peptide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HET7592390.1"
                     /translation="GVFALRPAPVQANWLAYPGTSGAPFIDYLIADRFLVPESARRFY
                     SEAIAYLPRCYQPSDPNRQVSHPPPRATFGLPDDAVVFASFNNAWKLNAASFARMCRA
                     LREVPGSVLWLLGAGRDADERLREAARTHGIGPGRLVFSPGLPHLQHLARYRLADLFL
                     DTNPYNAHTTASDAIWAGCPVLTRPGGTFASRVAGSLDHHLGMPELIAADDDAFVATA
                     ARLGNDRPVLAALRDRLAEQREASGLFDMRAYANDFAALLREMHEQHARGEAPGPIVP
                     RERGAR"
     gene            859..1380
                     /gene="moaB"
                     /locus_tag="VFK00_05240"
     CDS             859..1380
                     /gene="moaB"
                     /locus_tag="VFK00_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HET7592391.1"
                     /translation="MSAKNELVPLKIAVLTVSDSRTLENDSSGQFLADALAEAGHHLH
                     ARQLLRDDRYAIRAQVAAWIADADVHGVLITGGTGFTGRDSTPEAVLPLLDKEMPGFG
                     ELFRAVSFEEIGTSSLQSRAFAGLSNQTFVFALPGSTSACRTAWDKLLRAQLDARTRP
                     CNLAGLIPRLGES"
     gene            complement(1485..1751)
                     /locus_tag="VFK00_05245"
     CDS             complement(1485..1751)
                     /locus_tag="VFK00_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7592392.1"
                     /translation="MACARFLVAGRVQGVFFRASTREQALRLGLSGYAKNLADGRVEV
                     MASGSGAALAELEEWLREGPPGARVASVEREAMPEQILRGFQTK"
     gene            complement(1756..2247)
                     /locus_tag="VFK00_05250"
     CDS             complement(1756..2247)
                     /locus_tag="VFK00_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7592393.1"
                     /translation="MFSNFRAACVAVVLVLASGAAAAAAPASAAPPRLVVKTLGGRTF
                     DLASERGQWVIVNFWATWCSPCIAEMPAISKYVTTHKDVTAIGLAWDQSPRGDIVKFA
                     KEHPVDYPLAVLDPDHPPQGFPAPAALPTTYLIAPDGTVAKEFLGPITVKSLDAAIAA
                     KGS"
     gene            complement(2247..3527)
                     /locus_tag="VFK00_05255"
     CDS             complement(2247..3527)
                     /locus_tag="VFK00_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HET7592394.1"
                     /translation="MSSLSASAWHERRDRLRAFGHFLWRRFRDDNCFETAGALSYTTL
                     FAIVPLLSAVLAMLSLFPAFASLRDTLSNFIFRSFVPGAGDTIQGYLLKFADNASKLT
                     VVGIIVLLVSALLMMASIEDRLNRIWRVPARRKGGSRFLMYWAALTLGPVLIVLAVAA
                     SSWVFAQPFLAGASQPGVLGLSVIKLLPFASTWAGLALMYALVPNRRVRKRDALAGAL
                     AAAMLFELARFVFTWYVSSIANYNQVYGALAAIPIFLIWVFVSWVIVLLGATLTAALT
                     AFEYRTEDELLPEGCEFVGLLRVLHRFAAAQRNGRGLREAMLLECETFLTADLLQRYL
                     TDLREACLIQRDEAGAWVLGRDLNSVHLGDLFRIGGYRLPTDALSLQAAAGGLAPEAV
                     DVLLRAVTAERANLAHPLRSLFTAPKGAVEGEPS"
     gene            3697..4293
                     /gene="wrbA"
                     /locus_tag="VFK00_05260"
     CDS             3697..4293
                     /gene="wrbA"
                     /locus_tag="VFK00_05260"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HET7592395.1"
                     /translation="MDILVVYYSRNGHVAQLARLIARGIEEVENVHARVRQVPPVAPV
                     TETALPPVPEGGAPYVELKDLHECAGLIVGSPTRFGNMAAPMKHFLDSTPAEWVSGAL
                     VGKPCAVFTSTATMHGGQESTLLSMLLPLLHHGMLVVGIPFTEKALNATRTGGTPYGA
                     SHVSGVDGGNPISGDERELARALGRRVADVARRLAATP"
     gene            4290..4637
                     /locus_tag="VFK00_05265"
     CDS             4290..4637
                     /locus_tag="VFK00_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HET7592396.1"
                     /translation="MNARLAQRIGFTAWAALALLQVAWHGWLFAPATVPVAAALAIAL
                     VPLALPLAYWRQPQRALLAAGMVGLLYFCHGVAEAFAATAARPLAWIEIGLTVGLVLS
                     CARKPRRRKAIGN"
     gene            4910..6556
                     /locus_tag="VFK00_05270"
     CDS             4910..6556
                     /locus_tag="VFK00_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7592397.1"
                     /translation="MSFVQAAPQLGHPWREDRVLRAWVARVLPEPLRRDAGASFVALG
                     DYALHAYARQATTTPAEPVLTNWDVWGNRVDRIELTETWREGPAIAARQGLVADGHDR
                     ALGGFTRVQQFAKVYLYHAASAFYSCPLAMTDGVATALRDAPNAGLRERILPHLLSRD
                     PATFWISGQWMTENTGGSDVGASETVARREADGTWRLSGRKWFTSAINAEVALALARP
                     AGNPDGADGLALFCIETRNPDGSWNGISIDRLKDKLGTRELPTAEIHLQDTRAELVGD
                     TANGVRAIAPVLQVTRLWNAFGSLSTMQRCLVLVRDYADRRHAFGDSLRGQPLFADAL
                     AGWQAEFEAAFGLAMEVALLLGRVEAGEGDERDAALQRLLTPLAKLWICKLGVRIASE
                     TVEAVGGIGYLEDSGIPLLLRDAQVFPIWEGASNIQALDFLRALDKLGIEPLVGAVDR
                     WTEAADGAVSDRDAEAIHGAIRHADTWLRDHARDTDALQAGALRLGTTCARALAAALL
                     ARHAAWVQREQNDPSSAAALRRFCAHGLDRIIDFDLAGRG"
     gene            complement(6742..7893)
                     /gene="tgt"
                     /locus_tag="VFK00_05275"
     CDS             complement(6742..7893)
                     /gene="tgt"
                     /locus_tag="VFK00_05275"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7592398.1"
                     /translation="MHFELLATDGNARRGRISLPRGSVETPAFMPVGTYGSVKAMTPR
                     ALRDVGAQIILGNTFHLWLRPGREVIGEFGGLHSFIGWGAPILTDSGGFQVFSLAERR
                     KLTEEGVTFASPVDGSRVFLSPEESMRIQTVLDSDIVMAFDECTPYTVDGQPVDAGMV
                     RDSMELSLRWAARSRRAFDELGNGNALFGIVQGGVHGDLRARSAEGLLEIGFDGYALG
                     GLAVGEPEAERDATLEATLPRLPDDKPRYLMGVGRPEDIVEAVRRGIDMFDCVMPTRH
                     ARNGFLFTREGTLRIRNARFAKDTRPIEEGCTCYACANGFSRAYLRHLDRCGEMLGSQ
                     LATIHNLHHYLTLMRELREAIEAESLDRYVAEFYARRGGHGQPIPTQRC"
     gene            complement(8097..9167)
                     /gene="queA"
                     /locus_tag="VFK00_05280"
     CDS             complement(8097..9167)
                     /gene="queA"
                     /locus_tag="VFK00_05280"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7592399.1"
                     /translation="MKKSDFHYDLPVELIAQQPLPERSASRLLLVDAPQRALGDRSFN
                     DLPQLLQPGDLLVFNDTRVLPARLFGHKQSGGAVEIMLERIIGSHTALAMLGVSKKPR
                     EGDILELDDGTPVRVLGREGQFFDLRFECVEPLEKLLLRIGHMPLPPYIARPDDARDS
                     ERYQTVYARRPGAVAAPTAGLHFDAALLDTLRARGVQFGYITLHVGAGTFQPVRSENI
                     EDHEMHREWLNVGAELVEKIHRTRANGRRVIAVGTTVVRALEAARGDHGEVEPFAGET
                     RIFIFPGYRIASIDGLITNFHLPESTLLMLVSAFAGRDFVLSAYRHAVEQRYRFYSYG
                     DAMLVWPESRESGLGTRDPGAQ"
     gene            complement(9356..9958)
                     /locus_tag="VFK00_05285"
     CDS             complement(9356..9958)
                     /locus_tag="VFK00_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7592400.1"
                     /translation="MMRVLLAEDQAMVRGALAALLALEPDIEVLGSAADGESAWRELQ
                     RLKPDILISDIEMPGLTGLELAQRIQRQELPVKVVIVTTFARSGYLRRALDAGVSGYL
                     LKDAPAEKLADSLRMVHRGGRAIDPELALEAWTESDPLNDRERQVLRLAGEGKSAGEI
                     AQMFNLSHGTVRNYLSEAIGKLGVGNRIEAYRLARQKGWL"
     gene            complement(9955..>10145)
                     /locus_tag="VFK00_05290"
     CDS             complement(9955..>10145)
                     /locus_tag="VFK00_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7592401.1"
                     /translation="TGMRERIEAIGGTLAIESPRGRGTSIEVSLPIPVPAADGDRPAQ
                     APAATRDVPLVHQAAGRA"
BASE COUNT         1669 a         3515 c         3395 g         1566 t
ORIGIN      
        1 ggtgtgttcg cgttgcggcc cgcgccggtg caggcgaact ggctggcgta tcccggaacc
       61 tccggcgcgc ccttcatcga ctacctcatc gccgaccggt tcctggttcc ggaatccgcg
      121 cgccgcttct actcggaagc catcgcgtac ctgccgcgct gttaccagcc gtccgaccct
      181 aaccgccagg tctcccatcc gccgccgcgt gccacgttcg gattgcccga cgacgccgtg
      241 gtattcgcga gcttcaacaa cgcgtggaaa ttgaacgcgg cgagtttcgc acgcatgtgc
      301 cgggcgctgc gcgaggtgcc cggcagcgtg ctgtggctgc tcggcgcagg ccgcgacgcc
      361 gacgagcggc tgcgcgaagc ggcgcgcacc cacggcatcg gtccgggtcg gctggtgttc
      421 tcgcccgggc ttccgcacct gcaacacctc gcgcgctacc gcctggccga cctgttcctc
      481 gacaccaacc cctacaacgc gcacaccacc gcatcggatg cgatctgggc cggctgcccg
      541 gtcctgacgc gcccgggcgg aacgttcgcg tcgcgggtcg ccggcagcct cgaccaccat
      601 ctcggcatgc cggaactcat cgcagccgac gacgatgcat tcgtcgccac ggccgcccgc
      661 ctgggcaacg atcgcccggt gctggccgcc ctccgcgatc gtctcgcgga gcagcgggaa
      721 gcgtcgggtc tgttcgacat gcgcgcgtac gcgaatgact tcgcggcgct gctgcgtgaa
      781 atgcatgagc agcacgccag gggagaagcg ccggggccga tcgtgccgcg ggagcgcggc
      841 gcgcggtaag ctgagccgat gagcgccaag aacgaactcg ttccgttgaa gatagcggtg
      901 ctgaccgtct cggactcgcg cacgctggag aacgacagtt ccggccagtt cctcgcggat
      961 gcgctggccg aagccggaca ccatctgcac gcacgccagt tgctgcgcga tgatcgctac
     1021 gcgatccggg cgcaggtcgc cgcgtggatc gcggacgcgg acgtgcacgg cgtgcttatc
     1081 actggcggca ccggtttcac cggccgcgat tcgacgcccg aagcggtgct gccgctgctg
     1141 gacaaggaga tgccgggctt cggcgagctg ttccgtgccg tgagcttcga ggagatcggc
     1201 acatccagcc tgcagtcgcg cgcgttcgcc ggcctgtcca accagacctt cgtgttcgcg
     1261 ctaccgggtt cgacctcggc ctgccgaacc gcctgggaca aactgctgcg cgcgcagctc
     1321 gacgcccgca caagaccctg caacctcgcg gggctaattc cgcgactggg cgagagctga
     1381 agctgccttg ggcttctatt gcacgagtgg cgcagggccg cgaccttgtt gcccgggtcg
     1441 cgcgcatggc gcgctcctac attgattcgg ggccccgccg gtcatcattt cgtttggaat
     1501 ccgcgcaaga tttgctcggg catcgcctcg cgctcgaccg aggcgacgcg cgcgccgggc
     1561 ggcccttcgc gcaaccactc ttcgagttcg gccagtgccg cgccgctgcc cgacgccatg
     1621 acttccaccc gcccgtcggc caggttcttc gcgtagccgg aaagccccag ccgtagcgcc
     1681 tgctcgcggg tggatgcgcg gaagaacacg ccctgcaccc tgcccgccac caggaatcgc
     1741 gcgcaagcca tgttctcagg atcccttcgc ggcgatggcg gcatccagcg atttcacggt
     1801 gatgggcccc aggaactcct tcgcgacggt tccgtcgggc gcgatcagat acgtggtggg
     1861 gagcgccgcg ggtgccggaa agccctgcgg cggatggtcg gggtcgagca cggcgagcgg
     1921 gtagtcgacc gggtgctctt tggcgaactt gacgatatcg ccgcgcgggc tctggtccca
     1981 ggcgaggccg atcgcggtga cgtccttgtg ggtcgtgacg tatttcgaga tcgccggcat
     2041 ctcggcgatg cacggcgaac accaggtcgc ccagaagttg acgatcaccc attggccgcg
     2101 ctcgctggca aggtcgaagg tcctgccgcc cagggtcttc acgacgagcc gtgggggcgc
     2161 agcgctcgcg ggcgccgccg cagccgcagc gccggacgcc agtaccagga ctacggccac
     2221 gcatgcggca cggaagttgg aaaacatcac gatggctctc cttcgacagc ccccttgggt
     2281 gccgtgaaca atgacctgag gggatgggcg aggttcgccc gctcggcagt cacggcacgc
     2341 agcagcacgt cgacggcctc gggtgccagc ccgccggccg ccgcctgcaa cgacagtgcg
     2401 tcggtcggaa ggcggtagcc accgatccgg aacaggtcgc cgaggtgcac gctgttgagg
     2461 tcgcggccca gcacccaggc ccccgcctcg tcacgctgga taaggcacgc ctcgcgcagg
     2521 tccgtgaggt agcgctgcag caggtccgcc gtcaggaagg tttcgcattc gaggagcatg
     2581 gcttcgcgca agccgcgccc gtttcgctgc gcggccgcga accggtgcag cacgcgcagc
     2641 aggcccacga actcgcaacc ctcgggcagc agttcgtcct cggtccggta ctcgaacgcg
     2701 gtcagggcag cggtcagcgt ggcgcccagc agcacgatga cccacgacac gaacacccag
     2761 atcaggaaga tcgggatcgc tgccagcgcg ccatagacct ggttgtagtt cgcgatgctg
     2821 ctgacgtacc aggtgaatac gaagcgggcc agttcgaaca gcattgcggc ggcgagtgcg
     2881 ccggcgagag catcgcgctt gcggacccgg cgattgggaa ccagcgcgta catcagtgcc
     2941 aggccggccc aggtgctggc aaacggcagc agcttgatca cgctcaagcc caacacgccg
     3001 ggctggctgg cgcccgcgag gaacggctgg gcaaacaccc atgaactcgc ggcgaccgcc
     3061 agcacgatca gcaccggtcc gagcgtcaac gccgcccagt acatgaggaa acgcgaccct
     3121 cccttgcggc gggccggaac ccgccagatc cggttcagcc ggtcctcgat gctcgccatc
     3181 atcaacagcg cgctcacgag aaggacgatg atcccgacca cagtgagttt gctggcgttg
     3241 tccgcgaact tcagcaggta tccctggatc gtatcgccgg caccgggcac gaaactgcgg
     3301 aaaatgaagt tcgacaaggt gtcccgcagg gacgcgaatg cggggaacag cgacaacatg
     3361 gccagcacag cggacaacag cggcacgatc gcgaacaagg tcgtgtagga cagcgcgccg
     3421 gcggtctcga aacagttgtc gtcgcggaag cgccgccaga gaaaatgccc gaacgcccgc
     3481 aggcggtcgc ggcgttcatg ccatgcgctg gcagacaggg agctcacgct gcgagactag
     3541 tgcaatcggc gccggaaagc atgaccgcag gccgtgccgc gcgccccccg gggcgtggtt
     3601 cccgccgaga ccgataccct cgacgcgtgg tgtatgcgac catggacgac tttcggcggc
     3661 ggcgatgcgc tcccgccccg caagcccgga tcgtcgatgg acattctcgt cgtgtactac
     3721 agccgcaacg gccacgtcgc gcaactcgcg cgcctgatcg cgcgcggcat cgaggaagtg
     3781 gaaaacgtgc acgcacgggt gcgccaggtg ccccccgtgg cgccggtcac cgaaaccgcg
     3841 ctgccaccgg tcccggaggg cggcgcacct tatgtggaac tcaaggacct gcacgagtgc
     3901 gcaggactga tcgtcggcag cccgacgcga ttcggcaaca tggccgcgcc gatgaagcac
     3961 ttcctcgatt cgacgcccgc ggaatgggtt tccggcgcac tggtcggcaa gccgtgcgcg
     4021 gtgttcacct ccacggccac gatgcatggc ggccaggaat ccacgctgct gtcgatgctg
     4081 ctgccactgc tccaccacgg catgctcgtg gtcgggattc ccttcaccga aaaggcactc
     4141 aacgccaccc gtaccggcgg cacgccatat ggcgccagcc atgtgagcgg cgtcgacggc
     4201 gggaatccca tcagcggcga cgagcgcgag ttggcgcgtg cgctgggccg gcgggtggcc
     4261 gacgtggcgc ggcggctcgc agccacgcca tgaacgcgcg gttggcgcag cggatcggct
     4321 tcaccgcctg ggcggcgctc gcgctgctgc aggtcgcctg gcatggttgg ctgttcgcgc
     4381 ccgcgacggt gccggtcgct gcggcgctcg cgatcgcgct ggttccgctg gcgttgccac
     4441 tcgcgtactg gcgccagccg cagcgcgccc tgctcgcggc gggcatggtc ggcctgttgt
     4501 atttctgcca cggtgtcgcc gaagctttcg cggcgaccgc agcgcggcca ttggcgtgga
     4561 tcgagatcgg cctcaccgtc gggttggtgc tgtcctgcgc acgcaaaccg cggcgccgca
     4621 aggcaatcgg aaactgacgc aaggacacct gcgctgacac agggggcagt tgatcgcttt
     4681 gcgaaggctt ttcgacacgg accaaatccg acgaccacgg ataaagaacg tatatccgca
     4741 ttttttatcc gcatttatcc gcgtttatcc gcgtcaaaag cgtcctgtgg tcttgatgcg
     4801 aaaccctcac gggagccggt gggctcagcc gtcgccgacc acgcaacggc ttcgacatcg
     4861 cccacgcggc gcgttacgct cgacccactc ggccatgaag caagccgcca tgtcgttcgt
     4921 gcaagccgca ccgcagctcg gccacccttg gcgcgaggac cgcgtcctcc gcgcatgggt
     4981 tgcgcgcgtc ttgcccgagc cgctacgccg cgatgccgga gccagtttcg tcgcgctcgg
     5041 cgattacgcc ttgcatgcgt acgcgcgcca ggcgacaacc acgccggccg aacccgtgct
     5101 cacgaactgg gacgtctggg gcaatcgggt cgaccgcatc gaactgaccg aaacctggcg
     5161 cgaaggcccc gcgatcgccg cgcggcaggg actggtggcg gatggccacg accgcgcgct
     5221 cggcggattc acgcgcgtcc agcaattcgc caaggtctac ctctaccacg ccgccagcgc
     5281 gttctattcg tgcccgctgg ccatgaccga tggcgtcgca accgcacttc gcgatgcgcc
     5341 gaacgcgggc ttgcgcgaac gcatattgcc gcacctcctc agccgcgatc cggcgacgtt
     5401 ctggatcagc ggccagtgga tgaccgaaaa caccggcggc tccgacgtcg gggccagcga
     5461 gaccgtggcg cgccgcgaag ccgacggcac ctggcggctc agcggtcgca aatggttcac
     5521 ctccgcaatc aacgccgaag tcgcgctggc gctggcgcgg cctgccggaa atccggacgg
     5581 ggcggacggg ctcgcgctgt tctgcatcga gacccgcaac cctgacggaa gctggaacgg
     5641 catctccatc gaccgtctca aggacaagct aggcacccgt gaacttccga ccgccgagat
     5701 ccacctgcag gacacgcgcg cggagctggt tggcgacacc gcgaacggcg tacgcgcgat
     5761 cgcgccggtg ctgcaggtca cccggctgtg gaacgccttc ggttcgctgt ccacgatgca
     5821 acgctgcctc gtgctggtcc gcgactacgc ggaccggcgc cacgcgttcg gcgacagcct
     5881 gcgcgggcaa ccgctgttcg ccgatgcact cgccggctgg caggccgagt tcgaggcggc
     5941 cttcggcctc gcgatggagg ttgcattgct gctgggccgc gtcgaagcgg gcgaaggcga
     6001 cgagcgcgac gcggcgctgc agcggttgct gacgcccctc gcgaaactct ggatctgcaa
     6061 gctcggcgtg cgcatcgcgt cggaaaccgt ggaggcggtc ggcggtatcg gttacctgga
     6121 agacagcggt atcccgctgc tgctgcgcga tgcgcaggtg ttcccgatct gggaaggcgc
     6181 gagcaacata caggcgctgg atttcctgcg ggccctggac aagctcggca tcgagccgct
     6241 cgtcggcgcg gtcgaccgct ggacggaagc cgccgatggc gcggtgtccg atcgcgacgc
     6301 cgaagcgatc cacggcgcga tccggcatgc cgatacctgg ctgcgggacc acgcacgcga
     6361 taccgatgcc ctgcaagccg gcgcactgcg gctgggcacc acctgtgcgc gggccctggc
     6421 ggccgcgctg ctggcgcgcc acgccgcgtg ggtgcaacgc gagcaaaatg acccctcgtc
     6481 cgccgcggcg ttgcgacgct tctgcgcgca cgggctcgat cggatcatcg acttcgacct
     6541 tgccgggcgc ggctgaaaac ccatccccat ccccatcccg cccttcccct tgaaggggaa
     6601 ggagaccagc gcaaagcttt caagccctcc ccttcaaggg ggcgaatcga cgcgcttgcg
     6661 aaccgcaggt tcgtgcgtcg atgagcgcgc gaggcaggat gccgagggcc gggttgcttg
     6721 gcgagcgcag gacgcgcgag cttagcaacg ttgggtgggg atgggttgtc catgccctcc
     6781 acgccgcgcg tagaactccg ccacatagcg atccagcgac tccgcttcta tcgcttcacg
     6841 caactcgcgc atcagcgtca ggtaatgatg caggttgtgg atcgtggcga gctgcgaacc
     6901 gagcatctcg ccgcaacgat cgaggtgccg caggtaggcg cgcgagaatc cgttcgcgca
     6961 ggcgtaacag gtacagcctt cctcgatcgg gcgggtgtcc ttcgcgaacc gtgcgttgcg
     7021 aatgcgcaag gtgccctcgc gggtgaacag gaatccgttg cgggcgtgac gggtcggcat
     7081 cacgcagtcg aacatgtcga tgccgcgacg gacggcttcc acgatatcct ccggccgccc
     7141 gacgcccatg aggtaacgcg gcttgtcgtc cggcaggcgc ggcaacgtgg cttcgagggt
     7201 cgcgtcgcgc tcggcttccg gttcgcccac ggcgagcccg cccagcgcgt aaccgtcgaa
     7261 gccgatttcc agcaggcctt cggcagagcg cgcgcgcaag tcgccgtgca cgccgccctg
     7321 cacgatcccg aacaatgcgt tgccgttgcc gagttcgtcg aatgcccgcc gcgaacgcgc
     7381 cgcccagcgc aagctgagct ccattgaatc gcgcaccatg ccggcgtcca cgggctggcc
     7441 gtcgaccgtg tacggcgtgc attcgtcgaa cgccatcacg atgtccgagt cgagcaccgt
     7501 ctggatccgc atcgactcct cgggcgaaag gaatacgcgc gaaccatcca ccggcgaggc
     7561 aaacgtgacc ccctcttccg tgagcttgcg gcgctcggcc agggagaaca cctggaagcc
     7621 cccggagtcg gtgaggatcg gcgcccccca tccgatgaag ctgtgcagcc caccgaattc
     7681 gccgattacc tcgcgcccgg gccggagcca caggtggaag gtgttgccga ggatgatttg
     7741 cgcgcccacg tcccggaggg cgcgcggcgt catggccttg accgacccgt aggtgccgac
     7801 cggcatgaac gccggcgtct ccacggagcc gcgaggcagc gagatgcgac ctcggcgtgc
     7861 gtttccatcg gtggcgagaa gctcgaagtg catgattttt cgggacgcag actcgggact
     7921 cgggactcgg actcggactc ggactcggac tcggactcgg gaaggctaac aaaccccatc
     7981 gtcattccgg ggccggcgaa gccggaaccc ggaacctgcc ttgccctgct tacgcaacac
     8041 acggagcaga tcccggatcg cgactgcgtc gcgtccggaa tgacggccac aacaaatcat
     8101 tgcgcgcccg ggtcccgagt cccgagtccc gactccctgc tctccggcca caccagcatc
     8161 gcatccccgt aggaatagaa ccggtagcgt tgctcgaccg cgtggcggta cgccgacaac
     8221 acgaaatcgc gccctgcaaa cgccgacacc agcatcaaca gggtcgactc gggcaggtgg
     8281 aagttggtga tgaggccatc gatgctcgcg atgcggtagc ccggaaagat gaagatccgc
     8341 gtctcgcccg cgaagggctc gacctcgccg tggtcgccgc gcgcggcttc cagggcgcgc
     8401 accaccgtgg tgccgaccgc gatcacgcgc ctgccgttcg cacgcgtgcg atggatcttc
     8461 tcgaccagtt cggcgcccac gttcaaccac tcgcgatgca tctcgtggtc ttcgatgttc
     8521 tcgctgcgga ccggctggaa ggtccccgcg ccgacatgca gcgtgatgta gccgaactgc
     8581 acgccgcgcg cgcgcagcgt gtccagcaat gccgcgtcga agtgcagtcc cgcggttggc
     8641 gccgcgaccg cgcccggccg gcgcgcgtag acggtctggt agcgctcgct gtcgcgggca
     8701 tcgtctggcc gcgcgatgta gggcggcagc ggcatgtggc cgatccggag cagcagcttt
     8761 tccagtggct cgacgcattc gaaccgcaag tcgaagaact ggccctcgcg gccgagcacc
     8821 cgcaccggcg tgccgtcgtc cagttcaagg atgtcgccct cacgcggctt cttgctgacg
     8881 cccagcatcg cgagcgcggt gtgcgagccg atgatgcgct cgagcatgat ctcgaccgcg
     8941 ccgccgctct gcttgtggcc gaacagccgc gcaggcagca cccgcgtgtc gttgaacacc
     9001 agcaggtcgc ccggctgcag cagttgcggc aggtcgttga aactacggtc gcccagcgcg
     9061 cgttggggcg catcgaccag cagcagccgg ctggcgctgc gctcgggcag cggctgctgc
     9121 gcgatcaatt cgaccggcag gtcgtagtgg aagtcggatt tcttcaaggc cgggatgtag
     9181 gatcgggacc ggtaacaggg aaataggcaa tggtagcccg ccggccggcc agcgcgtctg
     9241 tggcgacgtg cagggaggta cggcgggcac tgcttgcccg gtcggaaaca cacgggtcgc
     9301 gaccatgggc cgctcccaca acaggcggcg cgccggtcgc ggccgcgggc cgctcctaca
     9361 gccagccttt ctggcgggcg aggcggtagg cctcgattcg gttgccgacg cccagcttgc
     9421 cgatcgcctc ggagaggtaa ttgcgcaccg tgccgtgcga gagattgaac atctgcgcga
     9481 tctcgcccgc gctcttgccc tcgccggcga ggcgcagcac ctggcgttcg cggtcgttca
     9541 gcggatcgga ttcggtccag gcttcgagcg cgagttccgg atcgatcgcg cggccgccgc
     9601 gatgcaccat ccgcagcgaa tccgcgagct tctcggccgg cgcatccttc agcaggtaac
     9661 cggacacgcc ggcgtccagc gcccggcgaa ggtagccgga acgcgcgaag gtcgtcacga
     9721 tcaccacctt caccggcagt tcctggcgct ggatgcgctg cgccagctcg agcccggtca
     9781 ggccgggcat ttcgatgtcg gaaatgagga tgtcgggttt caaccgctgc agttcgcgcc
     9841 acgccgactc gccgtccgca gccgagccca gcacctcgat gtcgggttcc agtgccagca
     9901 gcgcagccag cgcgccgcgc accatcgcct ggtcctcggc cagcagcacg cgcatcatgc
     9961 gcgaccagcc gcttggtgca ccagcggcac gtcccgggtc gcggccggcg cctgcgccgg
    10021 gcgatcgccg tccgccgccg gcaccggtat cggcaacgac acttcgatgc tggtgccgcg
    10081 tccgcgcggc gattcgatcg ccagcgtgcc gccgatcgcc tcgatccgct cgcgcatccc
    10141 ggtca
//
LOCUS       DASSNH010000143         7990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_18465, whole genome shotgun sequence.
ACCESSION   DASSNH010000143 DASSNH010000000
VERSION     DASSNH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18465"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFK00_05295"
     CDS             complement(<1..1024)
                     /locus_tag="VFK00_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7592402.1"
                     /translation="MFGYYLDLAWRSLKRTPILTGLMVLAIGLGIGASMTMLTVLHVM
                     SGDPLPGRSAHLYRPFLNPLPLSYKLPPQFIGHGPNAALTWPDAKALLDAHKGAQQVA
                     VGRGNLVAHSTQPGVHPESVVGSFTTRDFFAMFGVPFIAGDPWSEDADAEGAPVVVLT
                     QGFARKLFGDKQVLGKAVQLGKHVFRVVGVVKDWHPEPLFYAISASFDEADQFFLPLS
                     TAVHMKLDSNWTSGWGDDTSLTSPTTSWLNFWVRLDTPEQVADYGRFLVNYSAQQKAI
                     GRYERPPEDARLYGLMQWLHREGLVPTDVLLQSWLALGFLFVCMVNIIALLLAKFLRS
                     TGEISVR"
     gene            complement(1184..2404)
                     /locus_tag="VFK00_05300"
     CDS             complement(1184..2404)
                     /locus_tag="VFK00_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7592403.1"
                     /translation="MQIQPIVASLRKHRIPALLIVLEIALACAVLCNAVFMISQRVGE
                     MHVQNAMDEQGIIDMRVLGTDPKLAHADIPRDLAALREIPGVTAVAAVNMVPLGQNSW
                     NSNIATKPRDVMNKDNPNVAQYMFTRGGPEALGLRLVEGRFFNDGEYADSKLTESYLP
                     SGHSVLVTESLAERLWPGESALGKQAWIGRDNSFTVVGVVADVLRPNRNGEGAAGFHW
                     AMFFPLSPAAALNDYIVRSETQDRGRVLYAAQRKLEALSPNAVVKGKTFSDMRDQFFA
                     DTRSMVWMLVLVCVVMLAVTAFGIVGLTSFWVGQRRRQIGIRRAVGATRAHIMQYFQT
                     ENFMLSTAGVAIGMALAFGINLYLMQHYQTLRMPWYYLPASAVALWLLGQIAVLGPAL
                     RAANVPPVVATRGA"
     gene            complement(2575..3888)
                     /locus_tag="VFK00_05305"
     CDS             complement(2575..3888)
                     /locus_tag="VFK00_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7592404.1"
                     /translation="MFRYYLDLALRSFKRNRVLTALMVLALGLGIGASITTLAVLKLL
                     SGDPLPQKSARVLHPEIDPLPAQGYVADQTKPPWGGLMPYTDAMNLIRAHKAERQAAM
                     ALTQVKIEPQRTGADQKPFFSDGVMTTADFFAMFDAPFRYGTGWSEQDGEDRARVAVI
                     AGFLNDKLFGGKNSVGKTIRINGHDYRIVGVLEPWAPQPRFYAVGLGGRSYGNGDAVF
                     LPLQSARADGMDPQNVSCWEAADVKHLETSPCVFVYLWVEVRNPAAVAGYKTFLSNYV
                     QQQISLGRFHRPEVELPDVMQFMQEQQVVPDDVRLQAGLAFGFLLICVVNTVGLLLAK
                     CLRRSREIGVRRALGATRRTIFAQFMVESAIVGLAGGVLGLVFAELGLWAIRHQPAQY
                     ASLAHLDVAMFLFTFVVALAASLIAGLLPAWRACVVAPAPQLKSV"
     gene            complement(4117..5337)
                     /locus_tag="VFK00_05310"
     CDS             complement(4117..5337)
                     /locus_tag="VFK00_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7592405.1"
                     /translation="MQIRPILAALKHHKLATFLIAMEIALACGVLCNAVFLIADRLSA
                     MSLNSGVEEASLGEIVVTGFDPKQAGDLNARMLAGLGSIPGVESVHVVSSVPFGPQGG
                     VNGISLDEAGKHFGAVIQLYQGGPGTPAALGLKLVAGHLPASDDYQPIMGQDYMPLSS
                     RVLVTQALAEKLWPGQNPLGKEFWGAKYRFQVIGVLANLSVSQYTENGLHGAEWTAFV
                     PTRPGARLAGTYLVRAKPRDLERVMTQARASVARIAPDVVLDYSSSHTIGYLRQQFFK
                     TDRSMAGLLVGVIVALLGVTAFGIVGLASFWVAQRRKQIGIRRALGATRADILRYFQT
                     ENFLIVTFGVVLGIVLAIGINLGLMKFFEVPRLPLWYLPVGVAALWLLGQLAVLAPAL
                     RAAAVPPVVATRSV"
     gene            complement(5408..6733)
                     /locus_tag="VFK00_05315"
     CDS             complement(5408..6733)
                     /locus_tag="VFK00_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7592406.1"
                     /translation="MFAYYLDLAFRSLKRNKLLTVLMVIAIAIGIGASMTTLTVMHLL
                     SGDPLPGKSAHLYYPQVDPTPADWYNPRHPLPMMDYRSAVDLWSAQRADRQAIVVQSP
                     VKVSAPGIDRPPLMLTLMSTTTDFFPMFDVPFEYGSAWTVADEKDHARVAVISSDLND
                     KLFGGKDSVGKSLRLKDSNVRIVGVLAPWRPRPLFYDAVGGFTGNGFETSYYAKPEDV
                     FMPFFSGLDVNDGNFFQFTCWGGGPTTPGHLQDAPCLWTALWVELDSTAKAADYRAFL
                     GNYAERQKALGRFANTDTRMLDLMRWLDHEQVIPKNVKLQAWLAVAFLAICLFNTVGL
                     LLAKFLRRAGEIGVRRALGASRRSVFAQCLTEAGLIGFIGGVGGWLLTLVGLWLVRRQ
                     QTPYSDLVHMDVPMFVATFVLAVAVSLVAGALPALRASRIAPALQLKTL"
     gene            complement(6883..>7990)
                     /locus_tag="VFK00_05320"
     CDS             complement(6883..>7990)
                     /locus_tag="VFK00_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7592407.1"
                     /translation="ERNLFVIEQGWVGAPTGDDPASIEKLDAMQRSDLATLRQLPDVE
                     SAEAVSSMPLLQSSWTGGVGLKPDQQHSTTHAFYYFGGDQMLKTLGLRLLAGRNFTPD
                     EIQHRGFRSVGEPAMVIVTKALADKLFPKGGALGKTIYLDGNAVPSTIIGIVARLQAP
                     STGSWSNGFDYNSVLQPVRIDFQYTRTAVRAKPGRLDAAMREARKALYDANPMRVIDG
                     ELTQGLGIHPFSEIRTRAYRADVGMAVLMGVICAILLCVTGAGIVGLTSFWVGERRKQ
                     IGIRRALGATRRDILRYFQTENLLIAGIGVVLGAIIAVGLNLWMMKQFQMDRMSVLYV
                     VVGVGALLALGQGAVFAPALRASKVSPVEATRSV"
BASE COUNT         1476 a         2740 c         2443 g         1331 t
ORIGIN      
        1 ggcgtacgct gatctctccg gtcgagcgca ggaacttggc cagcagcagg gcgatgatgt
       61 tcaccatgca cacgaacagg aaacccaatg ccagccagga ctgcagcaac acgtccgtcg
      121 gcaccagccc ctcgcggtgc agccattgca tcaagccgta cagtctggcg tcctcgggcg
      181 gccgctcgta acggccaatg gccttctgtt gcgccgaata attgaccagg aagcggccgt
      241 aatcggcgac ctgctctggc gtatccaacc gcacccagaa attgagccaa ctggtggtgg
      301 gactggtcag ggatgtgtcg tcaccccagc cgctcgtcca gttcgagtcc agcttcatgt
      361 ggacggccgt ggacagcggc aggaagaact ggtcggcctc gtcgaagctc gcgctgatcg
      421 catagaacaa gggctccggg tgccagtcct tgaccacacc gacaacgcgg aaaacatgct
      481 tgccgagctg caccgccttg cccagcactt gcttgtcgcc aaacagcttg cgggcgaatc
      541 cttgcgtcaa caccaccacc ggggcgccct cggcatcggc gtcctccgac cacggatcgc
      601 cggcaatgaa cggcaccccg aacatggcga agaaatcccg ggtcgtgaag cttccgacca
      661 ccgactccgg atgcacaccc ggctgggtgg agtgcgccac gaggttgccg cggcccaccg
      721 cgacctgctg cgcgcccttg tgtgcatcca gcaaggcttt cgcatccggc caggtcaacg
      781 cggcgttcgg accgtggccg atgaactgcg gggggagctt gtaactgagc ggcaacggat
      841 tgaggaacgg gcggtacaaa tgcgcgctgc gtcccggcag gggatcgccg ctcatcacat
      901 gcagcaccgt gagcatggtc atcgaggcgc cgatgccgag cccgatcgcc agcaccatca
      961 atccggtcag gatcggtgtg cgcttcaggc tgcgccaagc gaggtcgagg tagtaaccga
     1021 acatgtccag atgcctccag gtttctcccc ctccccttca aggggagggg ctggggtggg
     1081 gatgggttgc cctgcagcgc actaatccac ccatcccctc ctaaatcctc cccttgaagg
     1141 ggaggacttc aaagccaccc gtccgccgag gggagggtga acatcacgcg ccccgcgtcg
     1201 ccactaccgg cggcacattg gcggcgcgca gtgccgggcc cagtaccgcg atctgcccga
     1261 gcagccatag cgcgaccgcg ctggccggca ggtagtacca aggcatccgg agcgtctggt
     1321 agtgctgcat caggtacaaa ttgatgccga acgccagtgc catcccgatc gcgacgccgg
     1381 cggtgctcaa catgaagttc tcggtctgga agtactgcat gatgtgcgcg cgcgtggcgc
     1441 cgaccgcgcg gcggatcccg atctgccgcc gccgctggcc cacccagaaa ctggtcagcc
     1501 ccacgatgcc gaacgcggtc accgccagca tcaccacgca caccagcacc agcatccaca
     1561 ccatgctgcg ggtgtcggcg aagaactggt cgcgcatgtc gctgaacgtc ttgcccttga
     1621 ccaccgcgtt cggcgacagc gcctcgagtt tccgctgcgc ggcgtaaagc acccggccac
     1681 ggtcctgcgt ctcgctgcgc acgatgtagt cgttgagcgc ggcagcgggg ctcagcggaa
     1741 agaacatcgc ccagtggaag ccggcggcgc cttcgccgtt gcggttgggg cgcaacacgt
     1801 cggccacgac acccacgacg gtgaagctgt tgtccctgcc gatccacgcc tgcttgccca
     1861 gcgcgctctc gcctggccac agccgctcgg ccaggctctc ggtgacgagg acggaatggc
     1921 cggaggggag gtagctttcg gtcagcttgc tgtcggcgta ctccccatcg ttgaagaagc
     1981 gcccctcgac caggcgcagg ccgagcgcct cgggcccgcc cctcgtgaac atgtactgcg
     2041 ccacgttcgg attgtccttg ttcatcacgt cgcgcggctt ggtcgcgatg ttggagttcc
     2101 aactgttctg gccgagcggc accatgttga ccgccgccac cgcggtgacg ccgggaatct
     2161 cccggagggc ggcaagatcc cgcggaatat cggcatgggc gagtttgggg tcggtgccga
     2221 gcacgcgcat gtcgatgatg ccttgctcgt ccattgcgtt ctgcacgtgc atctcgccga
     2281 cacgctggct gatcatgaac accgcgttgc acaggaccgc acaggcgagc gcgatttcca
     2341 gcacgatcaa cagggctgga atcctgtgct tgcgcagcga agcgacgatg ggttggattt
     2401 gcatggtgtt ttctcgatct attcgttcat tgccgatgta gcacggatgc aacgaaatcc
     2461 gcgctacgct cgcttttccc ctccccttca aggggagggc ggggtgggga tgggttgcca
     2521 gcgcgccgac atcaaaccca tccccatcca aaccttcccc ttgaagggga aggcttaaac
     2581 acttttcaat tgcggtgcag gcgccaccac gcaggcgcgc caggcgggca gcaatccggc
     2641 aatcaggctt gcggccagcg ccaccacgaa ggtgaacagg aacatcgcga cgtcgagatg
     2701 cgcgaggctc gcgtactgcg cgggctggtg gcggatcgcc cacaacccga gttcggcgaa
     2761 caccagcccc agcacgccgc cggccagtcc gacgatcgcg gattcgacca tgaattgcgc
     2821 gaagatcgtg cggcgcgtgg cacccagtgc ccgccgcacg ccgatttcgc gcgaacgcct
     2881 gaggcacttc gccaacagca ggcccacggt gttgactacg cagatcagca ggaacccgaa
     2941 cgcgaggccc gcctgcaggc gcacgtcgtc gggcaccact tgctgctcct gcatgaactg
     3001 catgacatcg ggcagttcga cttcgggcct gtggaagcgg cccagcgaaa tctgctgctg
     3061 cacgtagttc gacaggaatg tcttgtagcc ggccacggcc gcggggttgc gcacctccac
     3121 ccacaggtag acgaacacgc agggcgaggt ttccaggtgc ttgacgtcgg cagcttccca
     3181 gcaactgacg ttctgcgggt ccatgccatc ggcgcgcgcc gactgcaacg gcaggaacac
     3241 ggcgtcgccg ttcccgtaac tgcgaccgcc gaggcccacc gcgtagaaac ggggctgcgg
     3301 cgcccacggc tccagcacgc caacgatgcg gtagtcgtgt ccgttaatgc ggatggtctt
     3361 cccgacgctg ttcttgccgc caaacaactt gtcgttgagg aacccggcga tcaccgcgac
     3421 ccgcgcgcga tcctccccgt cctgctcgct ccagccggtg ccgtaccgga acggcgcatc
     3481 gaacatcgcg aagaagtcgg cggtcgtcat cacgccgtcg ctgaaaaacg gcttctggtc
     3541 cgcgccggtt cgctgcggtt cgatctttac ctgcgtcaac gccatcgccg cctgccgctc
     3601 cgccttgtgc gcacggatca ggttcatggc gtcggtgtac ggcatcagtc cgccccaggg
     3661 cggcttggtc tggtccgcca catagccttg cgcgggcagt ggatcgattt ccggatggag
     3721 cacccgcgca ctcttctgcg gcagcggatc gcccgacagc agcttcagca ccgccagcgt
     3781 cgtgatggat gcgccgatgc cgagccccag cgccagcacc atcagcgcgg tgagcacgcg
     3841 gttgcgcttg aagctgcgta gtgcgaggtc gaggtaatag cgaaacatta ttcacgctcc
     3901 aatttggtct ccctcccctt caaggggggg gccggggtgg ggatgggttt ccggaactgg
     3961 cgggccaacc ccatccccac ctaatcgttg ctaagctcgg acgtcctgtc ctcgcaaagc
     4021 aaccctgccc ttcgctcctg cttcgggcgc tcgcggcgcg cgaaccttcg gttcgcaaac
     4081 gccccgaccc gcccttgaag gggaggactt caaagctcac acgcttctcg tcgccaccac
     4141 cggcggcacc gccgccgcgc gcagcgcagg cgccagcacc gcgagttgtc cgagcagcca
     4201 caaggccgcc acgccgaccg gcaggtacca aagcggcagg cgcggcacct cgaagaactt
     4261 catcaaaccg aggttgatgc cgatcgccag cacgataccc agcacgaccc cgaacgtgac
     4321 gatcaggaaa ttttccgtct ggaagtagcg caggatatcc gcgcgcgtcg cacccagcgc
     4381 acgccggata ccgatctgct tgcgccgctg cgccacccag aaactcgcca gtcccacgat
     4441 tccgaacgcc gtgacgccta gcagcgccac gatcacgccg accagcaagc cagccatgga
     4501 ccgatcggtc ttgaagaact gctggcgtag gtaaccgatc gtgtggctgc tggaatagtc
     4561 gagcaccacg tccggcgcga tcctggcgac cgaggcgcgc gcctgcgtca tcacgcgttc
     4621 cagatcccgg ggcttggccc ggacgagata ggtgcccgcg agccgggcgc ccggccgggt
     4681 cgggacgaat gccgtccatt ccgccccgtg cagcccgttc tcggtgtatt gcgacaccga
     4741 caggttcgcc aggacgccga ttacctggaa ccgatacttc gccccccaga attccttgcc
     4801 caacgggttt tgacccggcc acaatttttc ggcaagggcc tgggtgacca ggacacgcga
     4861 cgagagtggc atgtaatcct gccccatgat gggctgatag tcgtcgctgg ccggcagatg
     4921 gcctgccacc agcttcagcc cgagagccgc cggcgtaccg ggccccccct ggtaaagctg
     4981 gatcaccgcg ccgaaatgct tgcccgcctc gtcgaggctg atgccgttga cgccaccctg
     5041 cgggccgaac ggcacggatg acaccacgtg caccgactcc acacccggaa tcgaaccgag
     5101 cccggcaagc atccgtgcgt tcaggtcgcc cgcctgtttc ggatcgaaac ccgtcaccac
     5161 gatctcgccc agcgaggctt cctccacgcc gctgttcaaa ctcattgccg aaagccggtc
     5221 ggcaatcagg aaaaccgcat tgcacaacac gccacacgcg agcgcgatct ccatcgcgat
     5281 caggaatgtc gcgagcttgt ggtgcttcaa agcggcgagg atgggtcgga tctgcatcat
     5341 cgaaacctcg gttggctcta cctggaggcg agcgccatgt ccgcgatctg ccgcgggcgc
     5401 ggccttccta caacgtcttc aactgcagcg ccggcgcaat gcggctggcg cgcaacgccg
     5461 gcaacgcgcc tgcaaccagg ctgaccgcca ctgcaagaac gaacgtcgcc acgaacatcg
     5521 gcacgtccat gtgcaccagg tcggaatacg gtgtctgctg ccggcgcacc agccacaggc
     5581 ccaccagcgt cagcagccag ccgccgacgc cgccaatgaa tccgatcagg ccggcttccg
     5641 tcagacattg tgcgaacacc gatcggcgcg acgcgcccag cgcccgccgc accccgattt
     5701 cgcccgcgcg ccgcaggaac ttcgccagca gcaagccaac cgtgttgaac aggcagatgg
     5761 ccaggaacgc aacagccaac caggcctgca gtttcacgtt cttcgggatc acctgctcgt
     5821 gatccagcca ccgcatcaaa tccagcatgc gggtgtcggt gttggcgaag cgcccgaggg
     5881 ctttttgccg ttcggcgtaa ttgccgagga aggcccggta gtccgcggcc ttggccgtgc
     5941 tgtccagctc cacccacagc gccgtccaca ggcagggcgc atcctggagg tgtcccggcg
     6001 tggtcggtcc gccgccccag caggtaaact ggaagaaatt gccgtcgttg acatcgaggc
     6061 ccgaaaaaaa cggcatgaac acgtcttccg gcttggcgta atagctcgtc tcgaacccat
     6121 tgccggtgaa cccgcctacc gcgtcgtaga acaacgggcg cgggcgccag ggggccaaca
     6181 cgcccacgat acgcacgttg ctgtccttca ggcgcagcga cttgcccacg ctgtccttgc
     6241 cgccgaacag cttgtcgttg agatcactgg agatcaccgc cacccgcgcg tggtcctttt
     6301 cgtccgccac ggtccacgcg ctgccatatt cgaatggcac gtcgaacatc ggaaagaagt
     6361 ccgtcgtcgt ggacatgagg gtcagcatga gcggcgggcg gtcgatgccg ggtgcgctca
     6421 ctttcacggg gctctgcacc acgatcgcct gacggtccgc gcgttgcgcg ctccacaaat
     6481 ccaccgccga acggtaatcc atcatcggca gcggatgccg cgggttgtac cagtcggcgg
     6541 gggtgggatc cacctgcggg taataaaggt gcgcgctctt gcccggcaac ggatcacccg
     6601 acagcagatg catcaccgtc agcgtggtca tgctggcgcc gatgccgatg gcgatggcga
     6661 tcaccatcag cacggtgagc agcttgttgc gcttcaagct gcgaaacgcc aggtcgaggt
     6721 aatacgcgaa catccatcac tccgattgcc ccttccgcag caaggggtgt tgtcttcccc
     6781 cttccacggg aagggggacc gagggggatg gctcttcgat ctccggagtc catccccccg
     6841 gcgcttgcgc gccgaccccc ttcccctgga aggaggtaat actcacacgc tgcgcgtcgc
     6901 ctccaccggc gacaccttcg acgcacgcag tgccggcgcg aacaccgcgc cctgccccag
     6961 tgcgagcagg gcaccgaccc ccaccaccac gtacagcacc gacatccggt ccatctggaa
     7021 ctgcttcatc atccagagat tcaaccccac cgcgatgatc gcgcccagca ccacgccaat
     7081 acccgcgatc agcaggtttt cggtctggaa gtagcgcagg atgtcgcgtc gcgtcgcacc
     7141 cagggcgcgg cggatgccga tctgcttgcg ccgttcgcct acccagaagc tggtgagccc
     7201 gacgatcccg gccccggtga cacacagcag gatcgcgcag atcaccccca tcaggaccgc
     7261 catgccgacg tcggcgcgat aggcgcgcgt acggatctca ctgaacggat ggatgcccag
     7321 cccctgggtt agctcgccgt cgatcacgcg catcggattg gcgtcgtaaa gcgccttgcg
     7381 cgcctcacgc atcgcagcgt ccagccttcc cggtttggcc cgcaccgcgg tgcgggtgta
     7441 ctggaaatcg atgcgcaccg gctgcaacac cgaattgtag tcgaagccgt tgctccagct
     7501 accggtactg ggcgcctgca ggcgcgcgac gatgccgatg atcgtgctgg ggacggcatt
     7561 gccatccagg tagatggttt tgcctagcgc accgcccttc ggaaacagct tgtcggccag
     7621 ggccttggtc acgatcacca tcgccggttc gccgacagag cggaagccgc ggtgctgtat
     7681 ctcgtccggc gtgaagttgc ggcccgcgag cagtcgcaac cccaaggtct tcaacatctg
     7741 gtcgccgccg aaataataga acgcgtgcgt ggtcgaatgc tgctggtccg gcttcaagcc
     7801 gaccccgccg gtccacgacg attgcagcag cggcatcgag ctgacggctt ccgccgattc
     7861 gacatcgggc aactgccgca gggtggcgag atcgctgcgt tgcatcgcgt ccagtttttc
     7921 gatggaggcc ggatcatcac cggtcggagc cccgacccag ccctgttcga tcacgaacag
     7981 gttccgttcg
//
LOCUS       DASSNH010000144         8531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_25016, whole genome shotgun sequence.
ACCESSION   DASSNH010000144 DASSNH010000000
VERSION     DASSNH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25016"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFK00_05325"
     CDS             complement(<1..268)
                     /locus_tag="VFK00_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7592408.1"
                     /translation="MTETIVITSGKGGVGKTTTSASLASGLAQRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVIQGEATLKQALIRDKRHDNLYVLAAS"
     gene            complement(357..1136)
                     /gene="minC"
                     /locus_tag="VFK00_05330"
     CDS             complement(357..1136)
                     /gene="minC"
                     /locus_tag="VFK00_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HET7592409.1"
                     /translation="MSGIAADTRAELLDAADLRFGQVGIASVRVRNDDPAALRASLEV
                     RVREAPQLFARAPVVVDLSFLAPQPSDEKIRGLLDGVRAAGMLPVGLAYGDAATEELS
                     QRLGLPLIAKFRAAYERAEPATPAEVQTSAAAGTAAPAAAAPAMSAPVPSVALQQHGS
                     IRTGQQVYARGADLVVVGAVANGAEALADGNVHVYGSLRGRAFAGALGDKATRIFCSE
                     FRAEIVSIAGHYRVFEELPKEFAGRAVQIWLEHDKLQVAAL"
     gene            complement(1133..1750)
                     /locus_tag="VFK00_05335"
     CDS             complement(1133..1750)
                     /locus_tag="VFK00_05335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592410.1"
                     /translation="MTFTPHLTLRDVRASDLAAVTGLNNTAGSSILPMDAAQIDHFYR
                     HADYFRVAEVDGHLAGFLIAFRDRTEHVSSNFRWFRRHCQEFVYIDRVVIGDLHRGHG
                     LGRLFYCDVQGFAEVRSSNLACEVFLEPRDDVSVLFHGVFGFHELGQQVMPESGRRVS
                     LLVKPLECHAFVRQHYPDGLPEVPWLAPRKRPADAVPHLLAAGQA"
     gene            1885..3090
                     /locus_tag="VFK00_05340"
     CDS             1885..3090
                     /locus_tag="VFK00_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7592411.1"
                     /translation="MTRPSLRPISLLRYAGLVTYACVGVPLARRDWMLANVSDERTQL
                     HVTRALHAQSHSDLLLWAASYAVFGVVYWLLTRDLGSRRFWGIKLLGLLVLTVAAVAI
                     SWYSVSGLGAILLAVAGMVLPWLLPLPVAIGWLVLQSAATVPVYATFPSFTLGMALMQ
                     AALYLGYSVIVFIASEVARQQADAREDQRRLNSELRATRALLAESSRISERMRIAREL
                     HDLVGHHLTALSLNLEVAGHLVANREAADHVARARSTAKQLLADVREVVSELRDDEGI
                     ELTTALRDLTEGVPGLEVHLELPQRFAVGDSRRAQILLRCTQEIITNAVRHARARNLW
                     LRFEEGPGGMLSLKARDDGRGALELKPGNGLTGMRERLAEVGGQLAIRTQRHEGFALE
                     ASLPTERIA"
     gene            3232..3873
                     /locus_tag="VFK00_05345"
     CDS             3232..3873
                     /locus_tag="VFK00_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002807809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7592412.1"
                     /translation="MISVCLVDDQTLVRQGIRSLLELSPEIQVVAECNDGASALETIP
                     KVRPDVVLLDMRMPGKSGLDVLNTFAAQGKVPPTIILTTFDDDQLVLAGLKAGARGYL
                     LKDVSLEQLVDAVKTVAGGGSLIAPVVTQRLLAGVGRIQNQFSSLGQPDPLTERETEI
                     LRLMSGGYSNKEIANSLKVAEGTVKNHVSNILSKLGVRDRTRAVLKALELGIV"
     gene            4011..5507
                     /locus_tag="VFK00_05350"
     CDS             4011..5507
                     /locus_tag="VFK00_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HET7592413.1"
                     /translation="MTRVLEFVVALIIVFVIAVIVGLFLPSHAHIERSLDISHNPQQI
                     YDVLNNFRRFPDYVGGELQAQDPQVKFSFSGPAYGPGATIHWAGGANGSVGKGTLVNK
                     SGNIDLAGNSTVTWALTNQWRGENKTFTLQVAPSHGQRVSAVTWGFDVDYGWNLIDRY
                     SQLWIHGVPDTMIQYGLDSLQTMLAGIPNVDYGKLAPGLYRTAPTPMLLVSTKATRTL
                     DDIDTAKAAALKEIEAAMDKLGVKAAGPTTTITTEWGDTTYTFDLAVPIDSTTIKVDG
                     KDYDLTRLPPRPTPEQMAKAASASSAASAASAAGAASAGNPAASGSAASGASAASSAS
                     AGEPASGSSSGAAASGDEDKGPVPGKLDKQNQLIVNDNVRAVMMPGREVLEATWTGEA
                     GIPLVRLALQAYAGTHGYDFNETEQRMYDELTSPVDTPDQDQIYRIFLPVTNAPAQTP
                     EQISGRAEPLTALDPSIWTGKEKNGEGEKNKKAEGRKPPAHAKKRHRG"
     gene            5959..6900
                     /locus_tag="VFK00_05355"
     CDS             5959..6900
                     /locus_tag="VFK00_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7592414.1"
                     /translation="MIETEHLGKRYGRFDAVADLTLKVAPGQVLALLGPNGAGKTTTM
                     RMIAGFLVPGSGCARVCGHDVVREPIKARRSLGYLPEGAPSYGELSVAEFLAFASRVR
                     GLSGIQGNRRLDEMIERLQLQPVLAQSIETLSKGYRRRVCLAQALLHDPPALILDEPT
                     DGLDPNQQREVHALIRGMARDRAIIVSTHALDEVRAACNRVAILAGGRLRVQGSPQEL
                     EQQSRYHHAVSFRAPASALACELLGRLPEVESVEVDAVDGRVTVFPRDRGDIFHVVVK
                     LLEEQRVRVDELQLERGRLEEVFGRITSDAESFARAA"
     gene            7037..7807
                     /locus_tag="VFK00_05360"
     CDS             7037..7807
                     /locus_tag="VFK00_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7592415.1"
                     /translation="MPRVAPAATDLRGHPFMGVLLRELRGYLITPVAWLFSVIFLLFA
                     GAFTFYLGAFFERGQADLDPFFRFHPWLYLVLVPALAMRLWAEERRSGTLELLMTLPI
                     RPWQAVLAKFFAAWIYIGCALALTFPMWLTVNYLGQPDNGVVLAGYIGSFLMAGAFLA
                     ISECLSVLTRSQVIAFILALAVCLLFLLAGDPLVQKPLLAIGSNRAADALAGLSLLAH
                     FQAIARGVLDFSDLVYFILTILFWLGANALILDARRGG"
     gene            7804..>8531
                     /locus_tag="VFK00_05365"
     CDS             7804..>8531
                     /locus_tag="VFK00_05365"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET7592416.1"
                     /translation="MRLRLPRISRLWLVLPVLAAVYVVLVLGASRWLDGKRVDLTRDH
                     LYTLSPGTLDIVAHLQQPLELTLYFSDRASHGLPQMRAYHQRVVSLLDEVVRRSHGRV
                     RFTEVDPSPFSEDEDRATAAGLTSVPSTTGEAIFFGLAAHNPADGKGAAIPFFLLAKE
                     RFIEYDLAKLLYDISTPHKPRVAIYSGLPIWGDGDAAAPWTALQQLRQLFDVHRLDSE
                     GLASLQADSVDALVLIQPVAMSQP"
BASE COUNT         1344 a         2840 c         2939 g         1408 t
ORIGIN      
        1 gcgaggcggc gagcacgtac aggttgtcgt ggcgcttgtc gcggatcagc gcctgcttca
       61 gcgtggcctc gccctggatc acgttcacga agtcgtacac cacgcgccgt tcgcagccca
      121 tgatgaggtc gaggttgcgc aagccgacgt cgaagtcgat gaccgcggtc ttgtggccgc
      181 gctgggcgag gccgctggcg agcgaggcgg acgtcgtggt cttgccgacg ccgcccttgc
      241 cggaagtgat gacgatggtt tcggtcaaga gggtgctccg agcttgggtt gggcggaagg
      301 atgccgcagg agtgggcgcc gcacggatga tccgggcgac gaatgaacgt gggatgtcac
      361 agcgccgcga cctgcagttt gtcgtgctcc agccagatct gcacggcgcg gcccgcgaat
      421 tccttcggca attcctcgaa cacgcgatag tggcccgcga tcgacacgat ctcggcacgg
      481 aattcgctgc agaagatgcg cgtggccttg tcgccgagtg cgcccgcgaa ggcgcgtccg
      541 cgcaggctgc cgtatacgtg gacgttcccg tcggccagcg cctcggcgcc gttggcgacc
      601 gcgccgacca ccaccaggtc cgcgccgcgc gcgtagacct gctgcccggt gcggatcgaa
      661 ccgtgctgct ggagcgccac gctcgggacg ggcgccgaca tggcgggcgc cgctgccgcg
      721 ggcgcggcgg ttccggccgc ggcactggtt tggacttcgg caggcgtcgc gggctcggcc
      781 cgctcgtagg ccgcgcggaa tttcgcgatc aacggaagcc cgagccgttg ggaaagttcc
      841 tcggtggcgg cgtccccgta ggccagcccg accggcagca tgccggcggc gcgcacgccg
      901 tccagcaagc cgcggatctt ctcgtcggac ggctgcggcg ccaggaagga aaggtccacc
      961 acgaccggcg cgcgcgcgaa cagttgcggc gcctcgcgca cgcgcacctc gagcgaggcg
     1021 cgcaacgcgg ccggatcgtc gttgcgcacg cgcacgctgg cgatgccgac ctgcccgaaa
     1081 cgcaggtcgg cggcgtcgag cagctcggcg cgggtatctg ccgcgatgcc gctcacgcct
     1141 gtccagccgc gagcaggtgg ggaaccgcat cggccggccg cttgcgcggc gcgagccagg
     1201 gcacctccgg caggccgtcg ggatagtgtt ggcgcacgaa cgcgtggcat tcgagcggct
     1261 tcaccagcag gctgacgcgg cgccccgatt cgggcatcac ctgctggccg agttcgtgga
     1321 agccgaacac gccgtggaac agcaccgaca cgtcgtcgcg cggctcgagg aacacttcgc
     1381 aggcgaggtt cgacgagcgc acttcggcga agccctgcac gtcacagtag aacaagcggc
     1441 caagaccgtg cccgcggtgc aggtcgccga tgaccacgcg gtcgatgtac acgaattcct
     1501 ggcagtgcct gcggaaccag cggaagttgg agctgacgtg ttcggtgcgg tcgcggaacg
     1561 cgatcaggaa cccggccagg tggccatcga cctccgccac ccggaaatag tcggcgtgcc
     1621 ggtagaagtg gtcgatttgc gccgcgtcca tcggcaggat ggaggatcct gccgtgttgt
     1681 tgaggcccgt gacggcggcg agatcgctgg cgcgcacgtc acgaagggtc aggtggggcg
     1741 tgaacgtcat cgtgtacggt attccgggtg ctggggccgg ctgccgggcc cgaaggcccg
     1801 ccattatcgc atgccttccg gcatacgtcc cgtccttcga cggatcgcac ggccaaccag
     1861 gcacggctgg gttaagatcg gcgcgtgaca cgtccatccc tccgtccgat atcgttgctg
     1921 cgctacgcgg gcctggtcac ctacgcctgc gtgggcgtgc cgctggcgcg gcgcgactgg
     1981 atgctggcca acgtcagcga cgagcgcacc cagctccacg tgacgcgcgc gctgcacgcc
     2041 cagagccact ccgacctgct gctgtgggcg gccagctacg cggtgttcgg cgttgtctac
     2101 tggctgctca cccgcgacct cggctcgcgg cgcttctggg gcatcaagtt gctgggcctg
     2161 ctggtgctca cggtcgccgc cgtggcgata agctggtaca gcgtcagcgg gctcggtgcc
     2221 atcctgctgg cggtcgcggg catggtcctg ccgtggctgc tgccactgcc cgtcgcgatc
     2281 ggctggctgg tcctgcaatc ggcggcgacc gtgcccgtat acgccacgtt cccctcgttc
     2341 accctgggga tggcgctgat gcaggcggcg ctgtacctcg gctattccgt gatcgtgttc
     2401 atcgcctccg aggtcgcgcg ccagcaggcc gacgcgcgcg aggaccagcg gcgcctcaat
     2461 tccgaactgc gggcgacgcg ggcgctgctc gccgagtcca gccgtatctc cgaacgcatg
     2521 cggattgcgc gggaactgca cgacctggtc gggcatcacc tgaccgcgct cagcctgaac
     2581 ctggaagtcg ccgggcatct ggtggccaac cgcgaggcag ccgaccatgt cgcgcgggcg
     2641 cgctcgaccg ccaagcaact gctggccgac gtgcgcgagg tcgtcagcga attgcgcgac
     2701 gacgagggta tcgagctgac cacggcgctg cgcgacctca ccgaaggcgt gccggggctg
     2761 gaggtccatc tcgaattgcc gcaacgtttt gcggtcggcg attcgcgacg tgcgcagatc
     2821 ctgctccgtt gcacccagga aatcatcacc aatgccgtgc gccatgcccg cgcgcgcaat
     2881 ctgtggttac gcttcgagga aggtcccggc ggcatgttgt cgctgaaagc gcgcgacgac
     2941 ggtcgcggcg cgctcgaatt gaagccgggc aatgggctga cgggcatgcg cgagcggctc
     3001 gccgaagtgg gcggccaact ggcgatccgc acccagcggc acgagggatt cgccctggaa
     3061 gcatcgctgc cgacggagag gatcgcgtga gccggaccgg gtcggcgcct tcggaaagtc
     3121 gcgggccgga catgtacact ccggttcctg cgttccgtcc gcaccgccgc gcgtggggat
     3181 tcttcgcaag ggcatggccg ggaccgatcg agtcgttggg ggaggggttg catgatttcc
     3241 gtttgcctgg tggatgacca gacactggtc cggcagggca tccgttcgct gctggagttg
     3301 tcgcccgaga tccaggtggt cgccgaatgc aacgacggcg cgtcggcgct cgaaacgatc
     3361 ccgaaggtgc gcccggacgt ggtgctgttg gacatgcgca tgccgggcaa gagcggcctc
     3421 gatgtcctca acaccttcgc tgcgcagggc aaggtgccgc cgacgatcat cctgaccacc
     3481 tttgacgacg accagctcgt gctggccggg ctgaaggccg gcgcccgggg ctacctgctg
     3541 aaggatgttt cgctcgagca gctcgtcgac gcggtgaaga cggtcgccgg cggcgggagt
     3601 ctgatcgcgc cggtcgtgac ccaacgcctg ctggcaggcg tcggccgaat ccagaaccag
     3661 ttctcgagcc tcggccagcc cgatcccttg accgaacgcg aaaccgagat cctgcgcctg
     3721 atgtccggcg gctacagcaa caaggagatc gccaattcgc tgaaggtcgc ggagggaacg
     3781 gtcaagaacc acgtgtccaa catcctttcc aagctcggcg tgcgcgaccg gacccgggcg
     3841 gtgctgaagg cgctggaact cgggattgtc tagggcgcat gccgtagcgc gaaccccgtg
     3901 aagcaagtac catccgcctt tcatgtgttt cacagcggcg cacgagctgc cgacccggcc
     3961 ggtcgcagcg ggcgtcggcc atacgtacaa gccgtccgga gattcctggc atgacccgtg
     4021 tgctcgaatt cgtcgtcgcc ctgatcatcg tgttcgtcat cgccgtgatc gtgggcctgt
     4081 tcctgcccag ccacgcgcac atcgagcgct cgctggacat cagccacaac ccccagcaga
     4141 tctacgacgt cctcaacaat ttccggcgtt tcccggacta cgtgggcggt gaattgcagg
     4201 cacaggatcc gcaggtgaag ttctcgttct cgggccccgc ctatggcccg ggcgcgacga
     4261 tccactgggc cggcggcgcc aacggcagcg tcggcaaggg cactctggtc aacaagtccg
     4321 gcaacatcga cctcgccggc aacagcaccg tgacctgggc cctcaccaac cagtggcgcg
     4381 gggagaacaa gaccttcacc ctgcaggtgg cgcccagcca tgggcagcgc gtcagcgccg
     4441 tgacctgggg cttcgacgtc gactacggct ggaacctgat cgaccgttat tcgcaattgt
     4501 ggatccatgg cgtgcccgac acgatgatcc agtacgggct cgactcgctg cagacgatgc
     4561 tggcgggtat tcccaacgtc gattacggca agctcgcgcc ggggctgtat cgcaccgcgc
     4621 cgacgccgat gctgctggtc tcgaccaagg cgacccgcac gctcgatgac atcgacaccg
     4681 ccaaggccgc ggcgctcaag gagatcgagg ccgcgatgga caagctcggg gtcaaggccg
     4741 ccgggcccac caccaccatc acgaccgagt ggggcgacac cacctatacg ttcgacctgg
     4801 cggttccgat cgattccacc accatcaagg tcgatggcaa ggattacgac ctgacccggt
     4861 tgccgccgcg gccgaccccg gagcaaatgg ccaaggcggc ttccgcttcc tctgcggctt
     4921 ctgctgcctc tgctgccggc gcggccagtg ccgggaatcc tgccgcctcc ggcagcgcgg
     4981 cgtcgggcgc cagtgcggcc tcgtcggcga gcgcgggaga gccggcctcc ggatcgtcgt
     5041 cgggtgcggc cgcgtcgggt gacgaagaca agggcccggt cccgggcaag ctcgacaagc
     5101 agaaccagtt gatcgtgaac gacaacgtcc gcgcggtgat gatgccgggc cgcgaagtgc
     5161 tggaggccac ctggaccggc gaagccggga tcccgctggt gcgcctggca ttgcaggcct
     5221 atgccggcac ccacggctac gatttcaatg aaaccgagca gcgcatgtac gacgagctga
     5281 cgtcgcccgt cgacacgccg gaccaggacc agatctaccg gatcttcctg ccggtgacca
     5341 acgcgcccgc gcagacgccg gaacagatct ccggtcgcgc cgaaccgctc accgcgctgg
     5401 atccgtccat ctggacgggc aaggaaaaga acggcgaagg cgaaaagaac aagaaggccg
     5461 aggggcgcaa gcctccggca cacgcgaaga agcgccatcg cggctgagcg acgtgatcgt
     5521 ctgatgtgat cttctggaga ggcccgcttt gcgggccttt cctttttgcg cagttcgatc
     5581 ggcgcggccc gggccgatcc gacttccgta gagcgcgcca tgcgcgcgaa gcgccggtgt
     5641 cggcgcactc gccatcgcgg gaagaagaag gagcaagccc ttcgcgcagg gccgctcctg
     5701 caagtcccgg tggggccagc attcgtccat ggtgcgtggc gccggcccgc acatcccggt
     5761 gcgggcgttc gccgacgcac gcgatgcccc cggcatcgcg caaacggtgc gttggcgagt
     5821 agccatccat ggcgccgggc gccggtgcgg ccatccatgg ccgcccgttc gaagcggcgc
     5881 gtgatgccac tggcatcccg cgagcgccac ttctcaccct gctagagttt gcgctcgcaa
     5941 tcgcgtgact cgctggcgat gatcgaaacc gaacacctcg gcaagcgtta cggcaggttc
     6001 gatgccgtgg ccgacctgac cctgaaagtg gcgcccgggc aggtgcttgc gctgctcggc
     6061 ccgaacggcg ccggcaagac caccacgatg cggatgatcg cggggttcct ggtgcccgga
     6121 tccgggtgcg cgcgggtctg cgggcatgac gtcgtgcgtg aacccatcaa ggcacgccgc
     6181 tcgctggggt atttgcccga aggtgcgccg agctatgggg aactctcggt ggcggagttc
     6241 ctggcgttcg cttcgcgggt gcgcggcctg agcggcatcc agggcaatcg ccgcctcgac
     6301 gagatgatcg agcgcctgca actccagccg gtgctggcac agagcatcga gacgctttcg
     6361 aagggctacc gccggcgcgt gtgcctggcc caggcgttgc tgcacgaccc gccggcactg
     6421 atcctcgacg agcccaccga cgggctggac cccaaccagc agcgcgaagt gcatgcgctg
     6481 atccgcggga tggcgcgcga ccgcgcgatc atcgtttcga cgcacgccct cgacgaggtg
     6541 cgcgcggcct gcaaccgggt ggcgatcctc gctggcgggc ggctgcgggt gcagggctcg
     6601 ccgcaggaac tcgagcagca gtcccgctac caccacgcgg tgagtttccg ggcgccggcc
     6661 agcgccctcg cttgcgagtt gctgggacgg cttcccgagg tggagtcggt ggaagtcgac
     6721 gccgtcgatg gccgcgtcac cgtgtttcca cgcgatcgcg gggacatctt ccacgtcgtg
     6781 gtgaagttgc tggaggagca acgggtacgc gtcgacgaat tgcaactcga gcgtggccgc
     6841 ctcgaggaag ttttcggacg gatcaccagc gatgccgaaa gcttcgcgcg tgcggcatga
     6901 accgcgaggg tgatccgccg atgaacgcaa agaacgcaaa tggaagcgag cgggaaacgc
     6961 ggctggatac gcgtccgttc gagtttgttt gcgggccgtg ctgctgggtc tcctggtcgc
     7021 ggacgcaggt cgtggcatgc cgcgcgtagc accggcagcc accgacctgc gcggccatcc
     7081 attcatgggt gtgctgctgc gcgagctgcg cggttacctc atcacgccgg tggcgtggtt
     7141 gttcagcgtg atcttcctcc tgttcgccgg cgcgttcacg ttctacctcg gcgccttctt
     7201 cgagcgcggc caggccgacc tggacccatt cttccggttc cacccgtggt tgtacctggt
     7261 gctggtaccg gcgctggcaa tgcggctgtg ggccgaggaa cgccgcagcg gcacgctcga
     7321 actgttgatg acgctgccga tccggccgtg gcaggcggtg ctcgcgaagt tcttcgcggc
     7381 atggatctac atcggctgcg cgctggccct cacgttcccg atgtggctca cggtcaacta
     7441 cctcggccag ccggacaacg gcgtggtgct ggccggctac atcggcagct tcctgatggc
     7501 gggtgcattc ctcgcgatca gcgagtgcct gtcggtgttg acccgtagcc aagtgatcgc
     7561 gttcatcctc gcgctcgccg tgtgcctgct gttcctgctt gccggcgatc cgctggtgca
     7621 aaagccgctg ctggcgatcg gctcgaaccg ggcggccgac gcgttggcgg gtctcagcct
     7681 gctggcgcat ttccaggcga tcgcacgcgg cgtactcgat ttctccgacc tcgtgtactt
     7741 catcctgacg atcctgttct ggctcggcgc aaacgccctg atcctcgatg cgaggcgcgg
     7801 cggatgagac ttcgcctgcc gaggatcagc cgcttgtggc tggtactgcc ggtacttgcc
     7861 gccgtctacg tggtgctggt gctcggtgcg tcccgctggc tggacggcaa gcgcgtcgac
     7921 ctgacccgcg atcacctgta cacgctctcg ccgggcacgc tggacatcgt ggcgcacctg
     7981 cagcagccgc ttgagctcac gctgtacttc tccgatcgcg cgagccacgg cttgccgcag
     8041 atgcgcgcct accaccagcg cgtggtgagc ctgctggatg aggtcgtgcg gcgttcgcac
     8101 ggccgcgtgc gtttcaccga ggtcgatccg tcgccgtttt ccgaggacga ggaccgtgcc
     8161 accgccgcgg gcctgacttc ggtgccgagc accacgggcg aggcgatctt cttcgggctg
     8221 gccgcacaca atccggcgga cgggaagggg gcggcgatcc cgttcttcct gctcgccaag
     8281 gaacggttca tcgagtacga cctcgccaag ctgctctacg acatcagcac gccgcacaag
     8341 ccgcgggtcg cgatctacag cggcttgccg atctggggcg acggcgatgc ggcggcgccg
     8401 tggacggcgc tgcaacaact ccggcaattg ttcgacgtgc accgcctgga ctccgaggga
     8461 ctggcctcgt tgcaggccga cagcgtggac gcgctggtgc tgatccagcc ggtggcgatg
     8521 tcccagccgg a
//
LOCUS       DASSNH010000145        25617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_521410, whole genome shotgun sequence.
ACCESSION   DASSNH010000145 DASSNH010000000
VERSION     DASSNH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 25617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25617
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521410"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFK00_05370"
     CDS             complement(<1..606)
                     /locus_tag="VFK00_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7592417.1"
                     /translation="MDTIRIRGARTHNLKNIDLDLPRDQLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSSYARQFLSMMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGTPRCPNHGTPLAAQTVSQMVDQALALPEDRRFMLLAPVVRERKG
                     EHVQVFEQLRAQGFVRARVDGSVYDLDSVPALGLRIKHTIEV"
     gene            847..1167
                     /gene="rplU"
                     /locus_tag="VFK00_05375"
     CDS             847..1167
                     /gene="rplU"
                     /locus_tag="VFK00_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7592418.1"
                     /translation="MYAVILTGGRQYRVMQGEVLRVEKLDADPDAAVTFDQVLLVGEG
                     ESITVGAPHVAGATVTAKVRGHGRADKVRIVKFRRRKHHRKQMGHRQHYTEIEVTGIS
                     AGEK"
     gene            1180..1437
                     /gene="rpmA"
                     /locus_tag="VFK00_05380"
     CDS             1180..1437
                     /gene="rpmA"
                     /locus_tag="VFK00_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7592419.1"
                     /translation="MAHKKGVGSSRNGRDSNPKYLGVKLYGGQAVEAGNIIVRQRGTQ
                     FHPGTGVGLGRDHTLYALVDGKVEFTTKGPKSRRTVNVVGG"
     gene            1592..2626
                     /gene="obgE"
                     /locus_tag="VFK00_05385"
     CDS             1592..2626
                     /gene="obgE"
                     /locus_tag="VFK00_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7592420.1"
                     /translation="MKFVDEAIIKVQAGNGGDGCASFRREKFIPFGGPNGGDGGNGGS
                     VWLVADEGLNTLVDFRHQRQFKAQRGQNGMGSDMYGRGGEDTSIRVPVGTSVFSVDTD
                     ELIGDLTTHGQKLLVAKGGKGGLGNIHFKSSTNRAPRRFTSGTPGEERELKLELKVLA
                     DVGLLGFPNAGKSTFIRAVSAATPRVADYPFTTLQPHLGVVSLGPSQGFVVADIPGLI
                     EGAAEGAGLGIQFLKHVSRTSLLLHLVDVAPLDGADPVEQVRIIEGELQRHDPGLLGR
                     PRWLLLNKIDLWAPEEREANAREIIERLDWQAPWFTISAAERIGTREVCLAAQRFFDE
                     RKSEAGESAE"
     gene            complement(2710..2973)
                     /gene="rpsT"
                     /locus_tag="VFK00_05390"
     CDS             complement(2710..2973)
                     /gene="rpsT"
                     /locus_tag="VFK00_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7592421.1"
                     /translation="MANIQSAKKRARQSEVRRMRNASQRSMLRSTIRKVLKAIEAKDR
                     AGAEAAYKAAEPVLDRYASRGLIHRNKAARHKSRLAAHIKAIA"
     gene            3081..4697
                     /gene="murJ"
                     /locus_tag="VFK00_05395"
     CDS             3081..4697
                     /gene="murJ"
                     /locus_tag="VFK00_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7592422.1"
                     /translation="MTRQPSLLRGVFSFSSMTMISRVLGLARDISITHVFGVSAATDA
                     FVIAFRIPNFMRRLFAEGSFSTAFVPVFTEVKETRPREELRELTARVTGTLGGVLLVI
                     VALGLIFAPQVATGFAPGALDEPHKLDLTIKLLRLTFPYLLFVSLTALCGGVLNSFSK
                     FALPALAPVILNLCLIAGALWWSRYLAVPIEALGWAVLAAGILQLLFLLPSIRRLGLL
                     AWPRWGWSHPPVKKIMRLMLPTLFGSSVAQINLLLDTVVASLLITGSQTWLYQATRFL
                     ELPLGVFGVALGTVILPALSRHHVTADREGFSRSLDWGLRTTLLIAMPAMCGLLLLAE
                     PMVAAAFQNGNFTAFDSHMTAIAIVGMGCGVPAIALTRTLLPAFYSRQDTRTPVRAGV
                     IALVVNMVCNFALIALLFELWASAGLKQLPWLDGIARVPGLHLGMAIASSVSNYLQFA
                     LLWRYLKRAGVYQRQPGWMRHWLRIGVACAALLGVVAAGLWFWPWQQWTHEPIFTRVW
                     KLVALVCAGGAAYVAALYATGFRMRDLRAG"
     gene            4834..5664
                     /gene="panB"
                     /locus_tag="VFK00_05400"
     CDS             4834..5664
                     /gene="panB"
                     /locus_tag="VFK00_05400"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7592423.1"
                     /translation="MYVTPADAPKRKPVTVPGLRAMKSRGEKIVVLTCYDASFAAQME
                     AAGIDVALVGDSLGNVVQGQTSTVPVTLDQMVYHTSLVARGIRATLLVADLPFMQYRD
                     PATALAASARLMAEGGAAMVKLEGGADWTCEVIAALTSRDIPVCAHLGLTPQSVHKMG
                     GYRMQGKTDEAAARLLAESQAVARAGAELLVLESVPAALAGQITRELPIPTIGIGAGL
                     DCDGQVLVCYDMLGITPGRRPKFSKDFLAGRGSIRDAITAYGDDVRAGRFPAPEQIAG
                     "
     gene            5669..6514
                     /gene="panC"
                     /locus_tag="VFK00_05405"
     CDS             5669..6514
                     /gene="panC"
                     /locus_tag="VFK00_05405"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7592424.1"
                     /translation="MRTIADLRSLRAAVQAWKKDGQRVGFVPTMGNLHAGHYSLVGRA
                     RQGADRVVVSVFVNPTQFGPNEDYTRYPRTLAEDSVGLVEHQCDLLFAPAVETMYPDG
                     PGHALRVHVPDVGDTLEGEHRPGHFDGVATVVAKLLTMVQPDLAVFGAKDWQQLLVVR
                     RLVRELALPVEMVAGPIVREPDGLAMSSRNRYLDAGQRERAPQLHATLEWMRESFHEG
                     HARAAIEGAAMRRLERAGFAPDYAVVRRAEDLQVPSDDERTGLMALVAARLGETRLID
                     NLAFG"
     gene            6599..6985
                     /gene="panD"
                     /locus_tag="VFK00_05410"
     CDS             6599..6985
                     /gene="panD"
                     /locus_tag="VFK00_05410"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET7592425.1"
                     /translation="MQLNMLKAKIHRATVTHAELHYEGSIAIDGNLLDAAGIREFEQI
                     HAWNVNNGERFVTYALRAEEGSGIISVNGSAAHRAQPGDLVIIAAFVTLTEAEAEQHL
                     PKLIYVDAANHIARTNTSIPKQMAVA"
     gene            complement(7082..8185)
                     /gene="queG"
                     /locus_tag="VFK00_05415"
     CDS             complement(7082..8185)
                     /gene="queG"
                     /locus_tag="VFK00_05415"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET7592426.1"
                     /translation="MNIRMDPARETTAADIDFAGLAARIKAWGAELGFGKIGIAGVDL
                     HEDEAHLLAWLREGRHGTMDYMQRHGTRRSRPAELQPGTVRVISARMDYSPTGADAGA
                     AWQTLRDGDRAYIARYALGRDYHKLIRNRLQKLADRIAAETGPFGYRAFTDSAPVLEK
                     ALARNAGLGWVGKHTLLLDRDAGSWFFLGELYTDLPLPVDAPSTAHCGTCTRCIDVCP
                     TRAITGPYQLDARRCIAYLTIESKDAIPGELRPLIGNRVFGCDDCQLVCPWNKFAKPT
                     REEAFAPRHGLDGAKLAELFAWSEDEFLEKTEGMPIRRAGYEGFSRNVAVALGNAPPS
                     PEVVEALQGRAGDPSPLVREHVAWALAEQSRKV"
     gene            8208..9683
                     /locus_tag="VFK00_05420"
     CDS             8208..9683
                     /locus_tag="VFK00_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7592427.1"
                     /translation="MPKPDPQTALFTTAQVRALDRAAIDGGIPGIELMERAARAALEV
                     LRRRWPHARSLAVACGPGNNGGDGFLLAALAREAGLQVVVVAVGDHSSGDAAFARDRF
                     LREGGQVSPADSPLPDAEVYVDALFGSGLNRAPSGDAARLIAALNAQSKPVLALDVPS
                     GLDADTGVAFDPCVRAAATACFVGWKRGLFTAQGPDKCGARSLATLGISSGLFADEAP
                     DAMLLTPQALPPRPRDSHKGRYGHVLAIGGDLGAGGAVRMSGEAALRIGAGLVSIATR
                     EANVAAILAARPELMPHGVHVPRNLEPLLARASVIALGPGLGKEDWGRGLWQAALDAG
                     KPTVLDADGLNLLAELPRALPGRIVLTPHPGEASRLLGCSTGDVQADRFAAVRTLAEK
                     HRAVVVLKGEGSLVAGPDGRVALCRWGNPGMASGGTGDVLTGVVAGLLAQGLGAWEAA
                     CLGVGLHARAGDLAARAGERGLLATDLFPYLRALVNGRVGR"
     gene            9792..10268
                     /gene="tsaE"
                     /locus_tag="VFK00_05425"
     CDS             9792..10268
                     /gene="tsaE"
                     /locus_tag="VFK00_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7592428.1"
                     /translation="MAEDFESIDLPEEAATVALAHRLAPQVAEGGVLYLRGELGAGKT
                     TFARALLRALGVGERVKSPTYSLLERYTMHGRDAFHLDLYRIAEAGELEWLGLDELEE
                     SDAIVLVEWPERGAGALPAADLEILLEHRGTARRGRLEPLSPRGQGWIAAIGKRPR"
     gene            10391..11866
                     /locus_tag="VFK00_05430"
     CDS             10391..11866
                     /locus_tag="VFK00_05430"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7592429.1"
                     /translation="MRGTVRPLLSFLVLTCAALGCNAAIAAAASTVTQVRVWGGPDST
                     RVVLELTAPVDYKLFQLNKPDRVVLDLDNGAFGKGVGAKAGKGVVKDIRVGHFQGKAR
                     IVLDLERDAHPKSFLLPPAAQYGYRLVVDLGTPDGKTSRIVRTIDADIAEGKPRPVVI
                     AVDAGHGGDDPGARGRGGTLEKNVTLGIARELARLIDDQPGMHAVLTRKSDNYVPLEK
                     RFQIAREHKADLFVSIHANSCPDYCDARGASVWVLNPHGKQSEAGRWLAKSENASDLI
                     GGVSLDDKSHMLASVLLDLSQGASMHAAHEVGADVLTALGKIGPLYRDTVQGANFVVL
                     RSPDVPSILVETAFISNRTDERRLDSSKDREHLAKAVLDGVQQYFETTPPPGTWFASE
                     RNRRLHLTTSAATEVATRSKTAAGDVAIEPAALSTSAKKAIARADEAIQDMHKVARGE
                     TLSGIAQRYGVSMSSLRSANGGKIQSGGGIQAGQVLLIPNS"
     gene            12195..12271
                     /locus_tag="VFK00_05435"
     tRNA            12195..12271
                     /locus_tag="VFK00_05435"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12229..12231,aa:Asn,seq:gtt)
     gene            12384..13877
                     /locus_tag="VFK00_05440"
     CDS             12384..13877
                     /locus_tag="VFK00_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592430.1"
                     /translation="MSASAANPTPGISGMAVYVPPHRVDLAQWCGWTGNDPAKTRAVV
                     GHAFRMPAPGQNVYTLAANAVLRLIECYDIDPARIGYLALGTESSTDNAAGAVIVRGL
                     VDVALAAEGRPPIARDCEVPEFKHACLGGVYALKGGLRYLACDGRGRQAIAVSADIAE
                     YARGSSGEPTQGAGAVAMLLESPARLLELDLAAAGSASEYRGLDFRKPFLRFAGQTPG
                     QHGRLSDLPVFNGKYSTDCYTDATLGALRAMFARSGGSHAEYLRNLAAVFMHRPYHRM
                     PRSAWATAYLAAIVHDADAGDAEAARELGAFCAAADVSRESLSRELGSGAGACSEDGL
                     PEAAFPLSAAVVKAIRKSDAYPRIVDDKLQFGTAAMMELGNLYTAALPAWLAAGLDDA
                     AARGAELDGRDLLVLGYGSGDAAEAIPARVAPDWREAAAAIRFRDALADAIDLDSAEY
                     ALLHDSGSLDVAPVHPVPGLIIDRVGNRDDQDFQDYGVAYYRYCGPV"
     gene            complement(14037..14579)
                     /locus_tag="VFK00_05445"
     CDS             complement(14037..14579)
                     /locus_tag="VFK00_05445"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET7592431.1"
                     /translation="MYRKAKGFTLIELMMTLGVATILAMIGAPALGSLLARTREAGAE
                     ARVADTLRQARTAAVMDNTRIIVCPSDDGRHCRSGYDWQHGWIVAPDADGDEQPDDGH
                     PVIAALPAMAAGSRIITSAGRAHIIFHPNGSAAGSNARFTICHAREGDGKSVVVSNSG
                     RVRVAEPDPDRLQQCLAGIP"
     gene            complement(14832..18041)
                     /locus_tag="VFK00_05450"
     CDS             complement(14832..18041)
                     /locus_tag="VFK00_05450"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592432.1"
                     /translation="MNDTAAKRSDTVTLDPENPWLGLFSYSEETRAYFHGRDEEAAEL
                     ARRVQRKLLTVLFGQSGLGKTSLLRAGLVPRLRDEGFCPVYVRIDYAAESPPPSAQIK
                     QAVFRVTAAAGHWTRPGASVEGESLWEFLHHHGDLLRDVDGKPLMPLLIFDQFEEIFT
                     LGQANDAGRFRAKQFLEDLADLVENRPPAALEKRLEEDDDAVEQFDFARADYRVLIAL
                     REDYLAHLEGVKDIMPSITQNRMRLARMHGAQALSAVLKPGGKLVSQEVAESIVRFVA
                     GGSELINAEIEPSLLSLVCRELNTARRAQGRKEISADLLAGSRDTILSEFYERALADQ
                     PAGVRRVIEDELLTESGYRESLAEERVLKALADAGAAPDALANLVNRRLLRIEERLDM
                     RRVELTHDVLCSVVLASRNQRHAREAREAAERQLAEQREREAATHRALLRTRMIAAGC
                     LVLLVLAIVAAIFGFVGMHRAKTAQAEAQASRSNAEKLVSFLIEDFYSELEPTGRLET
                     LGKLAHMTVGYYDSLPPELVTPQTRIYRAMALVREGAAQQSSGNIDAAYKSFNDAQNV
                     FEKLRAGGDHGEAVTYGLALTLESRGYSIVGSSGGGLGTVAQLVQSADLLRPLVYGPH
                     PSRQVRQLYAQDLNILSHAQPPKTGVASCDEARKLLVGMGALDLSDLNATASYADVAD
                     SEARELLTLGRITDAQKLEQQVYDLAEKVLMQRPGDLHALADRSWAAELIGTLADRQH
                     DYATGAEYANRTVQAGEDEVRFNPSDLGGWQRWAVGLRQVSDQQFERGEVAKAIVTMQ
                     SLLALENDKRLPSRLGPLVWFQWIDLANLQAQTGDSAAAAQSLKNFARDVGEVIAPLA
                     ADDTRRKLLVDPGQSLEAALQLAEGAPQGAFRNASEVINRIEAIKTPRGTSGYLLKRN
                     LLGGMLDTASAAALRLGHYTHAESLARRWLALPVNPTSEADARNDSSRARATLAQAIA
                     MQGRNQEAKRILQPALAYYTSEQKAGADGTTFRHDYAYALYVSAIAEPNDAAATRQRE
                     AALNQSAALIGGASPEAQKMADMRRVSGLISAARTSPRG"
     gene            complement(18038..19426)
                     /locus_tag="VFK00_05455"
     CDS             complement(18038..19426)
                     /locus_tag="VFK00_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET7592433.1"
                     /translation="MSTLPAATDSAQAFDEDAWDDLLNYIEEHRVIPIIGPDLLRVQT
                     DSGLRPLYEWLAEKLAARLSVDPVGLPQPLTLNDVMCSYLGQHGRREEAYTRLRSIMR
                     ELHFDPPRPLRQLAQITDFDLFVTTTFDPLLETAVNLERYGGQPGTEVIAYTPNRVAD
                     LPAERADLSRTVVYHLLGRLSASPTYVLSDEDMLEFICALQSEHLTPEKLFHELEHNH
                     LLLIGSDFSNWLARLFLRMAKRKRLSDPRDFTEVFADDHTLQDARLVAFLQQVSVRTR
                     VYGGAEAFVAELHSRWSARQPEASTSGSDGSPQRFLPPAREMPENAIFISYAREDLPA
                     VQRLKAGLDAAGLTTWFDLDRLEGGDDYDRKIRANIARCSFFVPVVSASTQQRHEAYF
                     RREWSYAVDRTRNMAEGAVFTLPVCIDDTPEAAALVPNSFKAVHMVRLAGGEPTPEFV
                     RRLQELCRGSRP"
     gene            19540..21606
                     /gene="uvrB"
                     /locus_tag="VFK00_05460"
     CDS             19540..21606
                     /gene="uvrB"
                     /locus_tag="VFK00_05460"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7592434.1"
                     /translation="MDERTSQRADERFELVAPYRPSGDQPQAIEKLIEGFNAGLAAQT
                     LLGVTGSGKTFTIANVVQAVQRPTLVLAPNKTLAAQLYGEFKQFFPRNAVEYFVSYYD
                     YYQPEAYVPSSDTYIEKDASINDHIEQMRLAATKALLSRRDALIVGTVSAIYGLGDPE
                     DYLSLRLILSIGDRVDQRQLLRQLTELQYTRNEMELHRGTYRVRGETLDIFPAENETE
                     ALRIEMFDGEVEKISMFDPLTGQTLRKLPRCVVYPATHYASTRESVLNAIDTIKEELG
                     QRLEVLRGANKLLEAQRLEQRTLYDMEMMHEVGYCQGIENYSRHLTRRAPGEPPPTLF
                     DYLPSDALLVVDESHVTIPQLGAMYKGDRSRKETLVEFGFRLPSALDNRPLKFEEWEQ
                     REPRTIYVSATPGPYERRQSGGSVVELVVRPTGLVDPQVEVRPVRSQVDDVLGEIRER
                     VKLGDRVLITTLTKRMAENLTEFLAEQDVRVRYLHSDIETVERVEIIRDLRLGKFDVL
                     VGINLLREGLDMPEVSLVAIFDADKEGFLRSTGSLIQTIGRCARNLRGKAILYADTVT
                     KSIQAAIEETDRRRAKQVEYNTEHGITPTTIVRNIADVMEGAREDPSARGRGRARGKG
                     AAAQPSRKVAEQAADYKTLDARAAAALIKRLEAEMYRHAKDLEFEDAARLRDEIHRVR
                     EHALLQ"
     gene            complement(21654..23015)
                     /locus_tag="VFK00_05465"
     CDS             complement(21654..23015)
                     /locus_tag="VFK00_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7592435.1"
                     /translation="MMRRPRLLKDQRRDMVFPAELLAFAGFGAAILLVAWVPLWLERV
                     PLTLPMLAVGVGFLAFVFTRDAASPLAHPRAAKLFPEVVLIVAVMGAGLSIDRKFSWR
                     SWATSWRLLALVMPLSIAALAALGCALLGLTPGLAVLLAAILAPTDPVLASAVQVGLP
                     GSGEEGETRFALTSEAGLNDGMALPFLALGAAMIAHDSHPGAWILHWLGVDLIWGVVV
                     AALVGFGCGWLLVRGNRLLPERLQLTRSSEGVASVGVAFLVYAAAEAAHGYGFVAVFV
                     AAVAIRNFGHALDYAQRITESARRLERMLAFLVLGLFGGALASGVLAGVGWREVVFAL
                     LALLVVRPVTTLLGFVGSMHPAPVRLAAGYFGIRGLGSLYYIGYAAGAGALLEARYLW
                     SVMALTVLFSIVLYGTTAKYALGLLDRTMQQSGEAGPAPPANADMLAARERQPDGREP
                     RQE"
     gene            23274..24161
                     /locus_tag="VFK00_05470"
     CDS             23274..24161
                     /locus_tag="VFK00_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HET7592436.1"
                     /translation="MFAHNKRLQYSVHVAESNPGLANLMLEQFGGPDGELAAAMRYFT
                     QALGEDDPGRKDMLLDIATEELSHLEVIGTVVAMLNKGAKGELAEGANAAAEMYRSLH
                     GAGNDSHLTQILYGGGPALTNSGGVPWSAAYIDSRGDPTCDLRSNIAAESRAKMIYER
                     LINVTDDPGVKDALGFLMTREIAHQQSFEKALYAIQPNFPPGKLPGKPEFADVYVNTS
                     QGEGDMRGSWNSDDNFRYVSKAEEFLAMDGGDGKPKVQLTDHESRVLQELQQRTRSNP
                     KGNPKTGAELGIDGRAPRM"
     gene            24187..24723
                     /locus_tag="VFK00_05475"
     CDS             24187..24723
                     /locus_tag="VFK00_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7592437.1"
                     /translation="MSTARREMNEHDPARENLCDWLRNAHAMEMQAESMLKGRASRLE
                     HYPEMKARVEQHITETQQQALDVEQCLKSLGEDTSSLKDTGGKAMALMQAMGGIVMSD
                     EVIKGAIASYAFEHMEIASYTVLIAAARAAGETEVAHVCERILQEEKAMAAWLAEHAP
                     ATVQQYLQRERDGLESKR"
     gene            24946..25419
                     /locus_tag="VFK00_05480"
     CDS             24946..25419
                     /locus_tag="VFK00_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET7592438.1"
                     /translation="MQGMFTLGVPAWELACRAAIVYVALLILLRLSGKHTVGELSIFD
                     LIVVIVIGSAVRTALLGNDKSVLGGLLVVTVLLLLDYISAFLSARFRKAEHVLDGKPV
                     LIARDGVVFHDVLKQCNVAPGTFQSAMRSHSLTTAQIGQAILEANGNITFCRKPE"
BASE COUNT         4290 a         8360 c         8759 g         4208 t
ORIGIN      
        1 cacttcgatc gtgtgcttga tgcgcaaacc gagtgccggc acggaatcga ggtcgtacac
       61 gctgccgtcc acgcgcgccc gcacgaaacc ctgcgcacgc aactgctcga acacctgcac
      121 gtgctcgccc ttgcgctcgc ggaccaccgg cgccagcagc atgaaacgcc tgtcctccgg
      181 cagcgccagg gcttgatcga ccatctggct gaccgtctgg gcggccagcg gcgtgccgtg
      241 gttcgggcag cgcggcgtgc cgacgcgcgc gtacagcagg cggaggtagt cgtggatctc
      301 ggtgatggtg ccgaccgtcg atcgcgggtt gtgcgacgtc gacttctgct cgatcgaaat
      361 cgccggcgac agaccctcga tgtggtccac gtcgggcttt tccatcatcg acaggaattg
      421 ccgcgcgtat gacgacagcg attccacgta gcggcgctgg ccctcggcgt acagcgtgtc
      481 gaaggcgagc gaagacttgc ccgagcccga caggcccgta atcacgatca actgatcgcg
      541 cggcaggtcg aggtcgatgt tcttgaggtt atgggtgcgg gcaccgcgga tgcggatggt
      601 gtccatggaa tgctggcgac aaagcccgtt actatagcga cccgcgcaat gtggggattg
      661 cgtcgccggt tttcgatagc tgtattggct cccctctccc tccgggagag gcggccgtcg
      721 ccttcgcccg gagggagaag ggagaaactt gcccaatccc ctgaaatcgc gctagaatcg
      781 cgggttcgag cgcgccgctg cgcgcccgat ccacccagaa taacgacaga accagagagc
      841 caacccatgt acgcagtcat cctcaccggc ggtcgccagt accgcgtgat gcagggcgaa
      901 gtccttcgcg tcgagaaact ggatgccgac cccgatgccg cggtcacctt cgaccaggtg
      961 ctgctggtcg gcgaaggcga atccatcacg gtcggtgccc cgcacgtcgc gggcgcgacc
     1021 gtcaccgcca aggtgcgcgg ccacggccgt gccgacaagg tgcgcatcgt gaaattccgc
     1081 cgccgcaagc accatcgcaa gcagatgggc caccggcagc actacaccga aatcgaagtg
     1141 accggcatca gcgccggcga aaagtaagga gaagccgcca tggcacacaa gaagggcgta
     1201 ggttcctcgc gcaacggccg cgactccaac ccgaaatacc tcggcgtcaa gctgtacggc
     1261 ggccaggccg tcgaagccgg caacatcatc gtccgccagc gcgggaccca gttccatccg
     1321 ggtaccggcg tcggcctcgg ccgcgaccac acgctctatg cgctggtcga tggcaaggtg
     1381 gaattcacca ccaagggacc gaagagccgc cgcacggtga atgtggtcgg cggctgacgc
     1441 gcgcgccacg tcgaatccgg aaggccccgc ttcgagcggg gcttttcctt ttcgggaagc
     1501 gggaacaggg gacagggaac agggaacaga gagttggtgt tcccgtacca tgtgcgcgcg
     1561 cttcctgttc ccacttccct gttcccgctt tatgaagttc gtagacgaag ccatcatcaa
     1621 ggtgcaggcc ggcaacggcg gggacggttg cgcgagcttc cggcgcgaga aattcatccc
     1681 gttcggcgga cccaacggcg gcgatggcgg caatggcggc agcgtgtggc tggttgccga
     1741 cgagggcctg aacacgctgg tcgatttccg ccaccagcgc cagttcaagg cgcagcgcgg
     1801 ccagaacggc atgggcagcg acatgtacgg gcgcggcggc gaggacacat cgatccgggt
     1861 ccctgtcggc acctcggtat tcagcgtgga caccgacgaa ttgatcggcg acctcaccac
     1921 gcacggccag aagctgctgg tcgcgaaagg cggcaagggc ggcctcggca acatccactt
     1981 caagagttcc accaaccgtg cgccgcggcg attcacctcc ggcacgccgg gcgaggaacg
     2041 cgagctgaag ctggagctga aggtgcttgc cgacgtcggc ctgctcggtt tcccgaatgc
     2101 cggcaagagc accttcatcc gcgcggtctc ggcggcaacg ccgcgcgtcg cggattaccc
     2161 gttcacgacc ttgcagccgc acctcggcgt ggtcagcttg gggccctcgc agggcttcgt
     2221 cgttgcggac atcccgggct tgatcgaggg cgcggccgag ggtgcgggac tcggcatcca
     2281 gtttctgaag cacgtctccc gcaccagtct cttgctgcac ctggtcgatg ttgcgccgct
     2341 ggacggcgcc gatccggtgg aacaggtacg gatcatcgaa ggcgagctgc aacgccacga
     2401 tcccggactg ctgggccgcc cgcgctggct gctgctcaac aaaatcgacc tgtgggcgcc
     2461 ggaggaacgc gaagcgaatg cccgcgaaat catcgagcgc ctggactggc aggcgccttg
     2521 gttcacgatt tcggccgccg aacgcatcgg cacgcgcgaa gtctgcctcg cggcccagcg
     2581 gttcttcgac gagcgcaagt ctgaggcggg ggagtcggcc gagtgagcgg ccgggggaca
     2641 gcgcggcggg cagcgaaaaa gccggctggc gccggctttt tcaggttccc tgacgagcgt
     2701 cgctcgactt caggcgatgg ccttgatgtg cgcggccagg cggctcttgt ggcgcgcggc
     2761 cttgttgcgg tggatcaggc cacgactggc gtagcggtcg agcaccggct cggcggcctt
     2821 gtaggcggct tccgccccgg ccctgtcctt ggcttcgatg gccttcagca ccttgcggat
     2881 cgtcgagcgc agcatcgagc gctggctggc gttgcgcatg cggcgcactt cggactggcg
     2941 ggcgcgcttc ttggcggact ggatgttggc cacggggtga ctccggaaca atcgggggaa
     3001 atgaagccgg aaagtatcgc ggaatcgggg ctttcagtca atcctgcggt ccccggtccc
     3061 cgatccccga ggctggcggc atgacccgcc agcccagctt gctgcgcggg gtgttctcgt
     3121 tcagcagcat gacgatgatc tcgcgggtgc tggggctggc gcgcgacatc tcgatcaccc
     3181 acgtgttcgg ggtcagtgcg gcgaccgacg cgttcgtgat cgcgttccgg atcccgaact
     3241 tcatgcgccg gctgttcgcg gaagggtcgt tctcgaccgc cttcgtgccg gtgttcaccg
     3301 aggtcaagga aacccgccct cgcgaagagt tgcgcgagct gaccgcgcgg gtgaccggca
     3361 cgctgggcgg cgtgctgctg gtaatcgtgg cgctggggct gatcttcgcg ccgcaggtcg
     3421 ccaccggctt cgcaccgggc gcgctggacg aaccccacaa gctcgacctc accatcaagt
     3481 tgctgcggct gacgtttccg tatctgctgt tcgtgtcgct gaccgcgctg tgcggcggcg
     3541 tgctgaacag tttcagcaaa ttcgcactgc ccgcactcgc accggtaatc ctcaacctgt
     3601 gcctgatcgc gggcgcgctg tggtggtccc gttacctcgc cgtgccgatc gaggcgctgg
     3661 gctgggccgt gctggcggcc ggcatcctgc agttgctgtt cctgctgcct tcgattcgcc
     3721 ggctgggact gctggcctgg ccgcgctggg gctggagcca cccgccggtg aagaagatca
     3781 tgcgcctgat gctgccgacc ctgttcggct cgtccgtggc gcagatcaac ctgctgctcg
     3841 acaccgtggt cgcgtcgctg ctgatcacgg gctctcagac ctggctgtac caggccactc
     3901 gtttcctcga actgccgctg ggggtgttcg gtgtcgcgtt gggcaccgtg atcctgccgg
     3961 cgctgtcgcg gcaccacgtc accgccgacc gcgagggatt ttcccgatcg ctggattggg
     4021 gtctgcgcac gaccttgctg atcgccatgc ccgcgatgtg tggattgttg ctgctggccg
     4081 agcccatggt ggcggcggca ttccagaacg gaaactttac tgccttcgac agccacatga
     4141 ccgcgatcgc gatcgtcggg atgggctgcg gcgtacccgc gatcgcgctg acccgcacgc
     4201 tgttgccggc gttctattcg cgccaggaca cccggacccc ggtgcgcgcc ggcgtgatcg
     4261 cgctggtcgt gaacatggtc tgcaacttcg cgctgatcgc gctgctgttc gaattgtggg
     4321 cgtccgcggg attgaaacag ttgccgtggc tggacggaat cgcgcgggta cccggcttgc
     4381 atctcgggat ggcgatcgcg agttcggtat ctaactacct gcagttcgcg ctgttgtggc
     4441 gctatctgaa acgtgccggc gtgtaccagc gccagcccgg atggatgcgg cactggctgc
     4501 ggatcggggt ggcatgcgcg gcgctgctgg gggtggtcgc ggccggcttg tggttctggc
     4561 cgtggcagca gtggacccat gagccgattt tcacgcgggt gtggaaactg gtcgcgctgg
     4621 tctgcgcggg cggagcggcg tacgtggccg cgttgtacgc gaccggcttc cggatgcgcg
     4681 acctgcgggc aggttgattg ccggttggcg gcgggcggaa gggccgcgat aatgcgacga
     4741 tcgcggggag caggggcagg ccgacagccg cttgcttcgc atgcgtgttt ccctgctccc
     4801 ttcgcccttt ccgctgcctt tccggagtgc cgcatgtacg tcacccctgc cgacgccccc
     4861 aagcgcaagc ccgtgaccgt tcccggcctg cgcgcgatga agtcgcgcgg cgagaagatc
     4921 gtggtgctca cctgctacga cgccagcttt gccgcacaga tggaagcggc cggcatcgat
     4981 gtcgcgctgg tgggcgactc gttgggcaac gtggtgcagg ggcaaaccag tacggtgccg
     5041 gtgacgctcg accagatggt gtaccacacg tcgctggtgg ctcggggcat cagggcgacg
     5101 ctgctggttg ccgatctccc tttcatgcag taccgcgatc cggccacggc gctggcggcc
     5161 tccgcgcgac tgatggccga aggcggtgcc gccatggtga agctggaagg cggcgcggac
     5221 tggacctgcg aggtcatcgc cgcgctgacg agccgcgaca tcccggtgtg cgcgcacctc
     5281 ggcctgaccc cgcagtcggt gcacaagatg ggcggttacc ggatgcaggg caagaccgac
     5341 gaagcggccg cgcggctgct cgccgaatca caggcggtgg cgcgggcggg cgccgaattg
     5401 ctggtactgg aatcggtgcc ggcggcattg gcgggccaga tcacccgcga gttgccgatc
     5461 ccgaccatcg gaattggcgc cgggctcgac tgcgatgggc aggtgctggt gtgctacgac
     5521 atgctgggca tcacgccggg ccgccggccg aagttcagca aggatttcct ggccggccgt
     5581 ggctcgattc gggacgcgat cacggcctac ggcgacgacg tgcgcgccgg acggttcccg
     5641 gctcccgagc agatcgccgg ctgaaggcat gcgaacgatc gccgacctcc gatcgctgcg
     5701 cgccgccgta caagcctgga agaaggacgg ccagcgggtc ggttttgtcc ccaccatggg
     5761 caatctgcac gcgggccatt attcactggt cgggcgggcc cgccagggag cggaccgcgt
     5821 ggtggtcagc gtgttcgtga atccgaccca gttcgggccg aacgaggatt acacccgcta
     5881 cccgcgcacc ctcgccgaag acagcgtggg actggtcgag caccagtgcg acctgctgtt
     5941 cgcgcctgcg gtcgagacga tgtacccgga cggtcccggg catgcgctgc gcgtccacgt
     6001 gccggacgtc ggggatacgc tcgaaggcga gcaccggccc ggtcacttcg atggcgtggc
     6061 cacggtggtc gccaaactgc tgacgatggt ccaaccggac ctcgcggtgt tcggcgccaa
     6121 ggactggcag caattactgg tggtgcggcg gctggtgcgc gagctcgcgc tgccggtcga
     6181 aatggtcgcc ggacccatcg tccgcgagcc ggacggcctc gcgatgtcct cgcgcaaccg
     6241 ctacctcgac gccggccagc gcgagcgggc gccgcaactg cacgccaccc tcgaatggat
     6301 gcgcgagtcg ttccacgagg gccatgcgcg cgccgcgatc gagggcgccg cgatgcgccg
     6361 gctggagcgg gcgggcttcg cccccgatta tgcggtggtg cggcgcgcag aagacctgca
     6421 ggtgccgtcc gacgacgagc gcaccggctt gatggcgctg gtggcggcga ggctggggga
     6481 gacccgtttg atcgacaatc tggcgttcgg ctgaccgtcc ctccgttgtg cggtgcaaca
     6541 aaacggcggt caggcggtat aatggaaccg gttcacaggc cggaagccgg agccgaccat
     6601 gcaattgaac atgctcaaag cgaagatcca tcgcgcgacc gtgacccacg cggagctgca
     6661 ctacgagggc tcgatcgcga tcgacggcaa cctcctcgac gctgccggga tccgcgagtt
     6721 cgagcagatc catgcctgga acgtcaacaa tggcgagcgc ttcgtgacct atgcgctgcg
     6781 cgccgaggaa ggcagcggca tcatttccgt gaacggcagc gcggcgcatc gggcgcagcc
     6841 gggcgacctc gtgatcatcg cggcgttcgt gaccttgacc gaagccgagg ccgagcagca
     6901 cctgccgaag ctgatctatg tcgacgccgc caaccacatc gcgcggacca acacctcgat
     6961 cccgaagcaa atggccgttg cttgaggttc gtaggttggg cttctccggc tagccgggag
     7021 aagcccaaca tggtcgcggc gttgggcttc cccggatcga atccggaatc aacccaacct
     7081 gctaaacctt gcgggactgt tcggcgagcg cccacgcgac gtgctcgcgc acgaggggcg
     7141 acgggtcgcc ggcgcgccct tgcagcgctt cgaccacctc cggtgacggt ggcgcattgc
     7201 cgagtgcaac cgcgacgttg cgcgagaagc cctcgtagcc ggcgcgccgg atcggcatgc
     7261 cttccgtttt ctccaggaac tcgtcttcgc tccacgcgaa cagttcggcg agcttcgcgc
     7321 cgtcgaggcc atggcgcggc gcgaacgctt cttcccgtgt gggcttggcg aacttgttcc
     7381 aggggcagac gagctggcaa tcgtcacagc cgaacacccg gttgccgatc agcgggcgca
     7441 gttccccggg gatggcatcc ttcgattcga tcgtgagata ggcgatgcag cgccgggcgt
     7501 cgagctggta gggaccggtg atcgcccggg tcgggcacac gtcgatgcaa cgggtgcagg
     7561 tgccacagtg cgcggtcgat ggcgcgtcga ccggcagcgg caagtcggtg tacagctccc
     7621 ccaggaagaa ccacgaaccg gcgtcgcggt ccagcagcaa ggtgtgcttg ccgacccagc
     7681 cgagccccgc attgcgggcc agcgcctttt ccagcacggg tgccgaatcg gtgaaggcgc
     7741 gatagccgaa cggcccggtt tccgccgcga tccggtcggc gagtttctgc aggcggttgc
     7801 ggatcagctt gtggtagtcg cgaccgagcg catagcgcgc gatgtaggca cgatcgccgt
     7861 cgcgcagcgt ttgccacgcc gctccggcat ccgcgccggt cggcgaataa tccatgcgtg
     7921 ccgagatcac ccgcacggtc cccggctgca gttccgctgg ccggctgcgg cgggtgccgt
     7981 ggcgctgcat gtagtccatc gtgccgtggc ggccttcgcg cagccacgcc agcaggtgcg
     8041 cttcgtcctc gtgcaggtcg acaccggcga tgccgatttt cccgaagccg agctccgcgc
     8101 cccaggcctt gatgcgcgca gccaggcccg cgaaatcgat atccgcggcc gtggtttcgc
     8161 gggccggatc catgcgtata ttcatccgta cattgtagga tcacgcgatg ccgaagcccg
     8221 acccgcagac cgcgctgttc acgacggcac aggtgcgcgc gctcgatcgc gcggcgatcg
     8281 acggcggcat ccccgggatc gaattgatgg agcgcgcggc gcgcgccgca ctcgaagtat
     8341 tgcggcggcg ttggccgcat gcgcgcagcc tggcagtcgc gtgcggcccc ggcaacaacg
     8401 gcggcgacgg cttcctgctc gcggccctgg cgcgcgaagc cgggctgcaa gtcgttgtcg
     8461 ttgccgtggg cgatcattcg tccggcgatg ccgcgtttgc gcgggaccgt ttcctgcgcg
     8521 aaggcgggca ggtctcgccg gccgattcgc cgctacccga tgcggaggtt tacgtcgacg
     8581 cactgttcgg gtcaggactg aaccgtgcgc cctccgggga cgcggcgcgg ttgattgccg
     8641 cgctgaacgc gcaatcgaag ccggtgctcg cactggacgt gcctagcgga ctcgatgcgg
     8701 acaccggcgt cgctttcgac ccctgcgtgc gcgccgcggc gaccgcgtgc ttcgtcggct
     8761 ggaagcgggg actgttcacc gcgcagggac cggacaagtg cggcgcgcgt tcgctggcga
     8821 cgctcgggat ttcctcgggc ctgttcgccg acgaggcccc ggacgcgatg ctgctgacgc
     8881 cccaggcatt gccgccgcgg ccgcgtgaca gccacaaggg gcgctatggc cacgtgctgg
     8941 cgatcggcgg cgacctcggc gccggcggcg ccgtgcgcat gtccggcgaa gcggcactgc
     9001 ggatcggagc cggactcgtg agcattgcca cgcgcgaagc caatgtcgcg gcgatcctcg
     9061 cagcgcgccc ggaactgatg ccacacggcg tgcacgtgcc gcgcaacctg gaaccgctgc
     9121 tcgcccgtgc cagcgtgatc gcgctgggtc cggggctcgg caaggaggac tggggccgcg
     9181 ggctttggca ggccgcgctt gatgccggca agcccacggt gctcgatgcc gacgggctca
     9241 acctgctcgc ggagttgccg cgagccttgc ccggacggat cgtgttgacg ccccatccgg
     9301 gggaggcttc gcgcctcctg ggttgttcca cgggcgacgt gcaggcggat cgtttcgccg
     9361 ccgtgcggac gctggcggaa aaacatcgcg cggtggtggt gctgaagggc gaaggcagcc
     9421 tggtggccgg gccggatggc cgcgtcgcgt tgtgccggtg gggcaatccc ggaatggcca
     9481 gcggtggcac gggcgatgtg ctgacggggg tggtcgcggg cctgctggcg cagggtctcg
     9541 gcgcatggga ggcggcctgc ctgggcgtcg ggctgcatgc gcgcgccggc gacctcgcgg
     9601 cgcgtgccgg cgagcgcggg ctgctggcga cggatctgtt cccgtacctc cgcgcactcg
     9661 tcaacggccg ggtgggaaga tagaaaagct ccagacgcgg atcgagaacg gactgccgga
     9721 tccgcggttg gctgcgtcag gatgctctcc ggaggatggt ctgcgtgccg ccatttggcg
     9781 tgaggaattc gatggccgaa gatttcgaat cgatcgattt gcccgaggag gcggcgacgg
     9841 tggcgctggc gcaccggctc gctccgcagg tggcggaggg tggcgtgctg tacctgcgcg
     9901 gcgaactcgg ggcgggcaag accaccttcg cacgcgcgct gctgcgcgcg ctcggtgtcg
     9961 gggagcgggt caagagcccg acctacagcc tgctcgagcg ctacacgatg cacggacgcg
    10021 atgcgttcca cctcgacctg taccggatcg ccgaggcggg cgaactggaa tggctgggcc
    10081 tcgatgaact ggaagagtcc gacgcaatcg tgctggtgga gtggcccgag cgcggcgccg
    10141 gggcgctgcc ggcggccgac ctggagatcc tcctggagca tcgcggaacc gcgagacgcg
    10201 gtcgtctcga accgttgtcg ccgcgcggtc aaggttggat cgccgccatc gggaagcgcc
    10261 ctcgctagac gtctctttcg cccacttggc gtcactttct gaatcaatat tgataaatcg
    10321 atcgcagctc acggataaca aagggaaaag agcttgcaat ccggcccggc ttgcggttga
    10381 attcggggcc atgaggggga cagtccgccc attgctgtcg tttctggtcc tgacctgcgc
    10441 tgcactgggc tgcaatgcgg cgatcgccgc cgcggccagc acggtcacgc aggtgcgcgt
    10501 ctggggaggc cccgactcca cccgcgtggt gctggaactg acggcgcccg tggactacaa
    10561 gttgttccag ttgaacaagc cggatcgcgt ggtcctggac ctcgacaatg gcgccttcgg
    10621 caagggcgtc ggtgccaagg ccggcaaggg cgtggtcaag gacatccggg tcggccactt
    10681 ccagggcaag gctcggatcg tgctggacct cgaacgggac gcgcatccca agagtttcct
    10741 gctgccgccg gccgcccagt acggctaccg gctggtggtc gacctcggca cccccgacgg
    10801 caagacctcg cggatcgtgc gcaccatcga cgccgacatc gcggagggca agccgcggcc
    10861 ggtggtgatc gcggtcgatg ccgggcacgg cggcgacgat cccggtgcgc gtggacgcgg
    10921 cggcacgctc gagaagaacg tgacactcgg aatcgccagg gagctggccc gcctgatcga
    10981 cgaccagccc ggcatgcacg cggtgctgac ccgcaagagc gacaactacg tcccgctcga
    11041 aaagcgtttc cagatcgcgc gcgaacacaa ggccgacctg ttcgtctcga tccacgccaa
    11101 ctcctgtccc gattactgcg atgcgcgcgg cgcctcggtg tgggtgctga acccgcatgg
    11161 caagcagagc gaggcgggac gctggctcgc caagagcgaa aacgcatccg acctgatcgg
    11221 cggcgtttcg ctggacgaca agagccacat gctggcctcg gtgctgctgg atctttcgca
    11281 gggcgcgagc atgcacgccg cgcacgaggt cggtgcggac gtgctgaccg cgctgggcaa
    11341 gatcggaccc ctttaccggg acaccgtgca gggcgcgaac ttcgtggtgc tgcgctcgcc
    11401 cgacgtgccg tcgatcctgg tcgagaccgc gttcatcagc aaccggacgg acgaacgacg
    11461 cctcgacagc agcaaggacc gcgagcatct tgccaaggct gtgctggatg gcgtccagca
    11521 gtatttcgag accaccccgc cgcccgggac ctggttcgcc tcggaacgca accggcgcct
    11581 gcacctgacc acatcggcag cgaccgaggt cgcgacgcgc agcaagaccg cggccggcga
    11641 cgtggccatc gagccggcgg ccctcagcac gtcggcaaag aaggcgatcg cccgggccga
    11701 cgaggccatc caggacatgc acaaggtggc gcgcggcgag accttgtcgg gcattgccca
    11761 gcgctacggc gtcagcatga gttcgctgcg ctcggccaac ggcggcaaga tccagtcggg
    11821 cggcggcatc caggcggggc aggtgctgct gatcccgaat tcgtgacgcg atcgcccgtg
    11881 ctcgcttcgc ctggcgagta tccatgccct acgcgtatcg tcctcggcgt ttggaggagc
    11941 gcgccatccg cgcgacaggc gcttcggtga aaaaacgata ccggtcgcgg ccatgggccg
    12001 ctctacaatc cccgttttga cacctctcgt gccggccacg ataatgcacg gctcgctgcc
    12061 cgaatagctc agctggacga gcgtgcggcg tttgcgacag tccgggggac tgtcgcgacg
    12121 cacgcgaggg cgaggcaggg aagccgagcg gagtgcgctc catggatgga aacgaagcga
    12181 caacctcaca aattgcccga atagctcagc tggttagagc actagactgt taatctgggg
    12241 gtccttggtt cgagtccaag ttcgggcgcc agatcatcac aaggccggcg gaatccgtcg
    12301 gcctttttcg ttggcgcatc atggacgctt ttttcccgca tactcgcgcg cttcgttccc
    12361 gcaacaggaa tcggtacgtg gcagtgagtg cgtccgcagc gaatcccacc cctggcatca
    12421 gcggaatggc ggtatacgtg ccgccgcacc gcgtcgacct cgcgcaatgg tgcggctgga
    12481 ccggcaacga tcctgccaag acccgcgccg tggtaggcca cgcgttccgg atgccggctc
    12541 ccgggcagaa cgtgtacacg ctcgcggcca atgcggtgct gcgcctgatc gagtgctacg
    12601 acatcgaccc tgcgcgtatc ggctacctgg cgctcggcac cgaatcgagc accgacaacg
    12661 ccgccggcgc ggtcatcgtg cgtggcctgg tggacgttgc cctggcagcc gaaggtaggc
    12721 cgccgatcgc gcgggactgt gaggtgccgg agttcaagca cgcctgcctc ggcggggtgt
    12781 acgcgttgaa aggcggcttg cgctacctcg cctgcgatgg ccgggggcgg caggcgatcg
    12841 ccgtcagcgc ggatatcgcc gagtacgccc gcggcagcag cggcgaaccg acccagggcg
    12901 ccggcgcggt cgcgatgctg ctggaatcgc cggcgcggct gctggagctc gacctcgccg
    12961 ccgccggcag cgcgtccgaa taccgcgggc tggatttccg caaaccgttc ctgcggttcg
    13021 ccggccagac gcccggccag catggacgcc tcagcgacct gccggtgttc aacggcaagt
    13081 attcgaccga ctgctacacc gacgcgacgc tcggcgcgct tcgcgcgatg ttcgcgcgaa
    13141 gcggtggctc gcatgccgaa tacctgcgca acctggccgc cgtgttcatg caccggccct
    13201 atcaccgtat gccgcggagc gcgtgggcca cggcgtacct tgcggcgatc gtgcacgacg
    13261 cggacgcggg cgatgccgag gccgctcgtg aactgggggc gttttgcgcc gctgcggatg
    13321 tgtcgcggga aagtctgtcc cgcgaactcg gaagcggcgc cggtgcctgt tccgaagacg
    13381 gcctgccgga ggctgcgttc ccgctgtcgg cggcggtggt gaaggcgatc cgcaagagcg
    13441 acgcctaccc gcgaatcgtc gatgacaagc tgcagttcgg aacagccgcg atgatggaac
    13501 tcggcaatct ctacacggcg gccctgcccg catggctggc cgcgggactg gacgatgcgg
    13561 ccgcgcgagg cgccgaactc gatggccgcg atttgcttgt gctcggctac ggcagcggcg
    13621 atgcggccga agcgattccg gcccgggtgg cacccgactg gcgcgaagcc gccgccgcga
    13681 tccgcttccg cgacgcgctg gcggatgcga tcgatctgga ttccgcggaa tacgcgctgc
    13741 tgcatgacag cggttcgctt gatgttgcgc ccgtgcaccc ggtgcctggg ctgatcatcg
    13801 accgcgtggg caaccgcgac gaccaagact tccaggatta cggcgtcgcg tattaccgct
    13861 attgcgggcc ggtctgaaga aacgccgatc actgatccgg gggacgagct ctgccccacg
    13921 cggtttcgca ggagcgcgcc atgcgcgcga caggcgggtg gcgaagcgga ccgatcgcgg
    13981 ccgtgggccg ctcctgccga gccgtgcggg ggcgcatcgc ccgccgggcg tgccctttac
    14041 ggaattcccg caagacactg ctgcagcctg tccgggtcgg gctcggcgac cctgacgcgt
    14101 ccggagttcg acaccaccac ggacttgccg tcgccttcgc gtgcgtggca gatcgtgaaa
    14161 cgggcgttgc tgcctgcggc gctgccgttg ggatggaaga tgatgtgcgc gcggccggcg
    14221 ctggtgatga tgcggcttcc cgcggccatg gccggcagcg cggcgatgac cgggtggccg
    14281 tcgtcgggtt gctcgtcgcc gtcggcatcg ggcgcgacga tccagccgtg ctgccagtcg
    14341 tagcccgacc ggcaatgcct gccgtcatcg ctggggcaga cgatgatccg ggtgttgtcc
    14401 atgaccgccg ccgtgcgtgc ctgccgcagg gtgtccgcga ccctcgcctc cgccccggcc
    14461 tcgcgcgtgc gggcgagcag gctacccagc gccggcgccc cgatcatcgc gaggatcgtg
    14521 gccacgccca gggtcatcat caattcgatg agcgtgaagc ccttggcttt ccggtacatg
    14581 actgcttcct tcgtcgaagc aagcagtctg tggactcggg cgggggtgtg gcagcggcga
    14641 gaatcaccgg atggcgtcgg ggcgcgccta cgaaccgtgt aggccgcgcg aacccgcgga
    14701 atgtcgggcg catcgccaat aaaatgaaag gcgacggagg caggaagaaa aatcaactgc
    14761 ctccggtccc ttacggcgat cccctttttg gcgttcgatt ggcgccggga cgaccaggaa
    14821 ctagtttttc gtcagccgcg cggagatgtg cgggccgcgg aaatcagacc cgagacacgg
    14881 cgcatgtcgg ccatcttctg ggcttctggc gaagcaccgc cgatcagcgc tgcggattgg
    14941 ttcaacgcgg cctcgcgctg cctggtcgct gcggcatcgt tcggctcggc aatggcgctg
    15001 acatacaggg catacgcata atcgtggcgg aatgtggtgc catccgcgcc ggctttttgt
    15061 tcgctcgtgt aatacgccag cgccggttgc aggatcctct tggcctcctg gttgcgaccc
    15121 tgcattgcga tcgcctgcgc gagcgtggcg cgcgcgcggg aactgtcgtt tcgcgcgtct
    15181 gcttccgagg tcgggttgac cgggagggcg agccaccggc gcgccagtga ttcggcgtgc
    15241 gtgtagtgac ccagccgcag tgccgccgcc gacgccgtgt cgagcatccc gcccagcagg
    15301 ttgcgtttca gcagatagcc gctggtgcca cggggcgtct tgatcgcctc gatgcgattg
    15361 atcacctcgc tggcgttcct gaaagcgccc tgcggcgcgc cttcggcgag ctgcagcgcg
    15421 gcctccagcg attggcccgg gtccaccagc aactttcgcc gcgtgtcgtc cgctgccaga
    15481 ggcgcgatca cctcgcccac gtcgcgggcg aagttcttca gcgactgcgc cgctgcggcc
    15541 gaatcgccgg tctgtgcctg caggttcgcg aggtcaatcc attgaaacca cacgagcggc
    15601 cccaggcggg aaggcagccg tttgtcgttt tccagtgcca atagcgattg catcgtgacg
    15661 atggccttgg ccacttcgcc acgttcgaac tgctggtcgg acacctgccg caggcctacc
    15721 gcccagcgct gccatccgcc gagatccgat gggttgaagc gcacctcgtc ttcgcccgcc
    15781 tgcacggtgc ggttcgcata ctcggcgccg gtcgcatagt cgtgctggcg atccgccagg
    15841 gtgccgatca gctccgcggc ccaggaacga tccgccaggg cgtgcaggtc gccggggcgt
    15901 tgcatcagca ccttctcggc cagatcgtaa acttgttgtt cgagtttttg ggcgtccgtg
    15961 atacggccga gcgtcagcag ttcgcgggct tcggaatcgg caacgtcggc atacgacgca
    16021 gtggcgttga gatccgaaag atcgagcgcg cccatcccca ccagcagctt gcgcgcctcg
    16081 tcgcaactcg ccacgccggt cttcggaggc tgcgcatggc tgaggatatt caagtcttgt
    16141 gcatacaatt gccggacctg gcgcgaagga tggggaccgt acaccagcgg ccgcaacagg
    16201 tccgcggact ggaccaattg cgccaccgtg ccgagtccac cgccgctgct gccgacgatg
    16261 ctgtaacccc ggctttccag ggtgagcgcc aagccgtaag tcacggcttc gccgtggtca
    16321 ccgccggcgc gcagtttttc gaacacgttc tgcgcgtcgt tgaaactctt gtacgcggca
    16381 tcgatattgc cgctcgattg ctgcgccgca ccttcgcgca cgagcgccat tgcgcgatag
    16441 atccgggttt gcggcgtcac cagttccggt ggcaggctgt cgtaatagcc caccgtcatg
    16501 tgggcgagct tgcccagcgt ttccaggcgg ccggtcggtt cgagttcgga ataaaagtcc
    16561 tcgatcagga agctcacgag cttttcggca ttgctgcggg aggcctgcgc ctcggcttgt
    16621 gcggtcttcg cgcggtgcat gccgacgaag ccgaaaatcg cggcgacgat cgcgagcacc
    16681 agcagcacga ggcagccggc ggcgatcatt cgcgtgcgca gcagcgcgcg atgggtcgcc
    16741 gcctcgcgct cgcgttgttc ggccaactgg cgttcggcgg cttcacgggc ctcgcgcgcg
    16801 tggcgctggt tgcggctcgc cagcaccacg ctgcagagta cgtcgtgggt cagctccacc
    16861 cgccgcatgt cgaggcgttc ctcgatccgc aacagtcgac ggttgaccag gttggccagc
    16921 gcgtccggcg cggcgccggc atcggccagc gccttgagca cgcgttcctc ggccaggctt
    16981 tcgcggtagc ccgattcggt cagcaattcg tcttcgatca cgcgccgcac cccggccggc
    17041 tgatcggcca atgcgcgttc gtagaattcg gacaggatgg tgtcgcggga gccggccagg
    17101 aggtcggcgg aaatttcctt gcggccttgc gccctgcgcg cagtgttgag ttcgcggcag
    17161 accaggctca gcaacgacgg ttcgatttcg gcgttgatca gttccgaacc gccggcgacg
    17221 aatcgcacga tggattcggc gacttcctgc gagaccagct tgccgccggg tttcaggacc
    17281 gcgctgagcg cctgcgcacc gtgcatccgc gccagccgca tgcggttctg ggtgatggac
    17341 ggcatgatgt ccttgacgcc ttccaggtgc gcgagatagt cctcgcgcag cgcgatcagc
    17401 acgcggtaat ccgcgcgcgc aaaatcgaac tgctcgaccg cgtcgtcgtc ctcttccagg
    17461 cgtttttcca gtgcggccgg cggacggttc tccaccaggt cggcgagatc ttccaggaat
    17521 tgcttggcgc ggaaccggcc ggcatcgttg gcctggccga gcgtgaaaat ttcctcgaac
    17581 tggtcgaata tcagcaacgg catcagcggc ttgccgtcga catcacgcaa caggtcaccg
    17641 tggtggtgca ggaactccca tagcgactcg ccctcgaccg aggcacccgg ccgcgtccag
    17701 tggccggcgg cggcggtgac gcggaagacc gcctgcttga tctgtgcgga aggcggtggc
    17761 gattccgccg cgtagtcgat acgcacgtag accggacaaa atccctcgtc gcgcagtcgc
    17821 ggcaccaggc cggcacgcag caaggatgtc ttgccaagcc cggactgccc gaacagcacc
    17881 gtcagcaatt tgcgctggac gcggcgggca agctcggcgg cctcttcgtc gcggccgtgg
    17941 aaataggcgc gggtttcctc gctgtaggag aacaggccga gccagggatt ttccggatcg
    18001 agcgtgaccg tgtcgcttcg tttggccgcc gtgtcgttca tgggcgactc ccgcggcaca
    18061 gttcctgcag gcgccgcacg aattccggtg tcggctcgcc gccggccaag cggaccatgt
    18121 gcacggcctt gaaggagttt ggcaccagtg ccgcggcttc cggcgtatcg tcgatgcaca
    18181 ccggcagggt gaataccgcg ccttcggcca tgttgcgggt tcggtcgacg gcgtagctcc
    18241 actcgcgccg gaaataggct tcgtgccgtt gttgggtgga cgcggaaacc accggtacga
    18301 agaaactgca tcgcgcgatg ttggcgcgga tcttgcggtc gtagtcgtcg ccgccttcca
    18361 ggcggtcgag atcgaaccag gtggtcaagc cggcggcgtc cagaccggct ttcaggcgct
    18421 gcacggcggg gagatcctca cgtgcgtagc tgatgaaaat ggcgttctcc ggcatttcac
    18481 gcgcgggggg caggaaacgc tgcggtgagc cgtcggatcc actcgtggac gcttcgggct
    18541 ggcgcgcgct ccaacggctg tgcaactcgg cgacgaaggc ctcggcgccg ccatacacgc
    18601 gggtccgcac gctgacctgt tgcaggaatg cgaccagccg ggcgtcctgg agcgtgtggt
    18661 catcggcgaa cacttcggtg aagtcgcgcg ggtcggacag gcgcttgcgc ttggccatcc
    18721 tcaggaacag ccgcgccagc cagttggaaa aatcgcttcc gatcagcaac aggtggttgt
    18781 gttcgagttc gtggaagagt ttctccggcg tgaggtgttc ggactgcagc gcgcagatga
    18841 actccagcat gtcctcgtcg gacagcacgt aggtcggcga ggcggagagg cgtcccagca
    18901 ggtgatagac gaccgtgcgc gagaggtcgg cgcgctcggc cggcaaatcg gcgacgcgat
    18961 tgggcgtgta ggcaatcacc tcggtcccgg gctggccacc gtaacgttcc aggtttaccg
    19021 cggtttccag cagcggatcg aacgtggtgg tgacgaacag atcgaaatcg gtgatctgcg
    19081 ccagttgccg caacgggcgc ggcggatcga agtgcagctc acgcatgatg gagcgcagcc
    19141 gcgtgtacgc ctcctcgcgg cgcccatgct ggccgaggta gctgcacatc acgtcgttga
    19201 gcgtcagcgg ctgcggcagc cctaccgggt ccaccgacag gcgcgccgcc agtttttccg
    19261 caagccactc gtacagcgga cgcaggccgc tgtcggtctg cacgcgcaag aggtccggtc
    19321 cgatgatcgg gatgacccgg tgctcctcga tgtagttgag gagatcgtcc caggcgtctt
    19381 cgtcgaaggc ctgggcggaa tccgtcgcgg cgggcagcgt tgacatggcg gggaggccct
    19441 gttggccgca agagcttact cccaataatg ggcgatggcg caagcgccgt tccgaagcca
    19501 ggcggcgagg ccgccggccg gggcatttat aattaccgga tggatgaacg cacttcgcaa
    19561 cgcgccgacg agcgcttcga actggtcgcg ccgtatcggc cgtccggcga ccagccgcag
    19621 gcgatcgaga agctgatcga aggtttcaat gcgggcctcg cggcgcagac gctgctgggc
    19681 gtcaccggat ccggcaagac cttcacgatc gccaacgtgg tgcaggcggt gcagcgaccg
    19741 acgctggtgc tcgccccgaa caagaccttg gccgcgcagt tgtacggcga gttcaagcag
    19801 ttcttcccgc gcaacgcggt cgagtatttc gtcagctact acgactacta ccaaccggaa
    19861 gcctacgtgc cttcctcgga tacctacatc gagaaggacg cctcgatcaa cgaccacatc
    19921 gagcagatgc gcctcgccgc gaccaaggcc ctgctgtcga ggcgcgacgc gctgattgtc
    19981 ggcacagtgt ctgcgatcta cggcctgggc gatccggaag attacctgtc gctgcggttg
    20041 atcctctcga ttggcgatcg cgtcgaccag cgccagttgc tgcgccagtt gaccgagctg
    20101 caatacaccc gcaacgaaat ggaactgcac cgcggcacct accgggtacg tggcgagact
    20161 ctcgacatct tccccgccga gaacgaaacc gaagcgctgc ggatcgagat gttcgacggc
    20221 gaggtcgaga agatctcgat gttcgatccg ctgaccgggc aaaccctgcg caagctgccg
    20281 cgctgcgtcg tttacccggc cacgcattac gcctcgacgc gcgaaagcgt gttgaacgcg
    20341 atcgatacca tcaaggaaga gctggggcaa cggctggagg tcctgcgtgg cgccaacaaa
    20401 ttgctggaag cacagcgctt ggaacagcgc acgctctacg acatggagat gatgcacgag
    20461 gtcgggtact gccagggcat cgagaactac tcgcgacatc tgacccgtcg cgcgccgggc
    20521 gaaccgcctc cgacactgtt cgactacctg ccttccgacg cgttgctggt cgtcgacgaa
    20581 tcgcatgtca cgatcccgca gctcggtgcg atgtacaagg gcgatcgttc gcgcaaggag
    20641 acgctggtcg agttcggatt ccgcctgccg tcggcgctcg acaaccggcc gctgaagttc
    20701 gaggaatggg agcaacgcga gccccgcacc atctatgtgt cggcgacacc cggtccctac
    20761 gaacggcgcc agtccggcgg cagcgtggtg gaactggtag tgcgcccgac cggcctcgtc
    20821 gatccgcagg tcgaagtgcg accggtccgc tcgcaggtag atgacgtgct cggcgaaatc
    20881 cgcgagcgcg tgaagctcgg cgaccgggtg ctgatcacca cgctcaccaa gcgcatggcc
    20941 gaaaacctca cggagttcct ggccgagcag gacgtgcgcg tgaggtattt gcactcggac
    21001 atcgaaacgg tggaacgcgt cgagatcatc cgcgacctcc ggctcggcaa gttcgacgtg
    21061 ctggtcggca tcaacctgct tcgcgaaggc ctcgacatgc cggaggtatc gctggtcgcg
    21121 atcttcgatg ccgacaagga aggcttcctg cgctcgacag gatccctgat ccagacaatc
    21181 ggccgctgcg cgcgcaacct gcgcggcaag gcgatcctgt atgccgacac cgtgacgaag
    21241 tcgatacagg ccgcgatcga ggaaaccgat cgccgccgcg ccaagcaggt cgagtacaac
    21301 accgaacacg gcatcacccc gaccacgatc gtgcgcaaca tcgccgacgt gatggaaggt
    21361 gcccgcgaag atccatctgc gcggggtcgc ggacgtgcgc gcggcaaggg cgccgcggcg
    21421 caaccctcgc gcaaggtggc cgagcaggcg gccgactaca agacgcttga cgcccgcgcc
    21481 gccgccgccc tgatcaagag gctcgaagcc gagatgtacc ggcacgccaa ggacctggag
    21541 ttcgaggacg ccgcgcgcct gcgcgacgaa atccaccggg tgcgtgagca cgcgttgctg
    21601 cagtaggagc gggcagtgca tggcgcgccg cgcggttgcg cgactggatc ggctcattcc
    21661 tggcgcggct cgcgcccgtc cggctgccgc tcgcgcgccg cgagcatgtc cgcgttcgcc
    21721 ggcggggcgg gaccggcctc gccggattgc tgcatcgtgc ggtcgagcag gcccagcgcg
    21781 tacttcgcgg tggtgccgta gagcacgatg gagaacagca cggtcagcgc catcaccgac
    21841 cacaggtagc gcgcttcgag cagtgcaccg gcgccggcgg cgtagccgat gtagtacagc
    21901 gatccgagac cgcggatgcc gaagtaaccg gccgccaacc ggaccggtgc cggatgcatg
    21961 gatccgacga agccgagcag cgtcgtgacc ggacgcacca ccagcagggc cagcagcgcg
    22021 aacacgacct cgcgccagcc gacgccggcc agcacgccgc ttgcgagcgc gcctccgaac
    22081 aggccgagca ccaggaacgc gagcatgcgc tccagtcggc gcgccgattc ggtgatccgc
    22141 tgggcatagt cgagcgcatg cccgaagttg cggatcgcaa ccgcggcgac gaacacggcg
    22201 acgaatccat agccgtgcgc cgcttcggca gccgcgtaca ccaggaatgc cacacccacc
    22261 gacgcgactc cttcactgga ccgcgtcagt tgcagccgtt ccggcagcag ccggttcccg
    22321 cgcaccagca gccagccgca gccgaagccg accagcgccg cgaccacgac cccccagatc
    22381 aggtcgacgc caagccagtg caggatccat gcgccggggt gcgaatcgtg cgcgatcatc
    22441 gccgcgccga gggcgaggaa tggcagcgcc atgccatcgt tcaatccggc ctcggacgtc
    22501 agcgcgaagc gcgtttcgcc ttcctcgccc gagcccggca gcccgacctg caccgccgac
    22561 gccagcaccg ggtcggttgg cgcgaggatc gcggcaagca gcaccgccag tcccggcgtc
    22621 agcccgagca aggcgcagcc gagtgccgcc agtgccgcga tggacaacgg catcaccagc
    22681 gcgagcaagc gccagctcgt cgcccaactg cgccatgaaa atttccggtc gatgctgagg
    22741 ccggcgccca tcacggcgac gatcaggacg acttcgggaa agagcttcgc cgcacgcgga
    22801 tgcgcgagcg gcgatgcggc atcgcgggtg aacacgaacg cgaggaaacc gacaccgacc
    22861 gccagcatcg gcagcgtcag cggtacgcgc tccagccaca ggggaaccca cgccacgagc
    22921 aggatcgcgg ccccgaatcc ggcaaaggcg agcagttcgg cggggaaaac catgtcgcgg
    22981 cgttggtcct tcaacagccg ggggcgcctc atcatgctcg cgccccgcgg tccaggcaat
    23041 tcgcggcccc acggcttcgg ttgcgcaagc gtgctgcgcg cgtgcttctg agattgggat
    23101 ggttttgttc cggtgccgac tatggccggc gtgcggcaag ctcgcaaatt gttctgcaag
    23161 cccgcaagaa tttccgggaa tcaattcccc ggcacgcccc tcgcgcgagg ccgggatggc
    23221 acgcgtgttg cagcggcgta ggcaccgatg taatccaagg aagggccccg aacatgttcg
    23281 cgcacaacaa acgcctgcaa tattccgtac atgtagccga atccaacccc ggcctcgcca
    23341 acctgatgct cgagcaattc gggggccccg atggtgaact cgcggcagcg atgcgctact
    23401 tcacccaggc cctgggcgag gacgatccag gccgcaagga catgctgctg gacattgcca
    23461 ccgaggagct gagccatcta gaggtgatcg gcaccgtcgt ggcgatgctc aacaagggcg
    23521 ccaagggcga actggccgaa ggcgccaacg cggcggccga aatgtatcgc tcgctgcatg
    23581 gcgcgggcaa cgattcgcac ctgacccaga tcctgtacgg cggcggcccc gccctgacca
    23641 attccggggg cgtgccctgg agcgccgcct acatcgacag ccgtggggat cccacgtgcg
    23701 atctgcgctc caatatcgcc gcagaatccc gcgccaagat gatctacgaa cggctgatca
    23761 acgtcaccga cgatccgggc gtcaaggatg ccctagggtt cctgatgaca cgcgagatcg
    23821 cgcaccaaca gtcattcgag aaagcgctgt acgcgataca gcccaacttc ccgcccggca
    23881 agctgcccgg caagccggaa ttcgcggatg tctatgtcaa tacttcacaa ggcgagggcg
    23941 acatgcgggg atcgtggaac agcgacgaca atttccgcta cgtcagcaag gccgaagagt
    24001 tcctggcgat ggatggaggc gacggcaaac cgaaggtgca actgacggac cacgaaagca
    24061 gggtgctgca ggaattgcag cagcgcaccc ggtccaaccc gaagggcaat cccaagaccg
    24121 gagccgagtt gggaatcgat ggccgcgcac cgcggatgtg aaccggcaac aggacacgga
    24181 gtgtccatga gtaccgccag acgagaaatg aacgaacacg acccggcacg ggagaacctg
    24241 tgtgactggc tgcgcaatgc gcacgccatg gaaatgcagg ccgaatccat gctgaagggc
    24301 cgggccagcc gtctcgagca ctatccagaa atgaaggcgc gcgtcgaaca acacatcacc
    24361 gagacgcaac agcaggcgct cgacgtcgag cagtgcctga agtccctggg cgaagacacc
    24421 tcgtcgctca aggacaccgg cggcaaggcg atggccctga tgcaggcgat gggcggcatc
    24481 gtgatgtccg acgaagtgat caagggcgcg atcgcgagct atgcgttcga gcacatggaa
    24541 atcgcctcct acaccgtcct gatcgccgct gcccgggccg cgggcgagac cgaggtcgcg
    24601 cacgtctgcg agcgcatcct gcaggaggaa aaggcgatgg cagcgtggct ggccgagcac
    24661 gcgcccgcga cggtgcagca atacctccag cgcgaacgcg acggcctcga atccaagcgt
    24721 tgagcgcagc ggcaggttcc gccggcgttg cgcagtcccg aacccgaacg ccgccgggct
    24781 tcgaccatgc ccggcggcat tgaagcccac gccttcgcag cgtggtccag cacggcctgc
    24841 gcgagcaggc tgcgcgttcg tcgcagcgcg gttcgacggc ttggacgtgc gattgcccga
    24901 tggcaccagc cgccacggca gtcgcaatcg ccaaggaggc gttgtatgca agggatgttc
    24961 accctgggtg ttccggcgtg ggagttggcc tgccgcgcgg cgatcgtcta cgtcgcgctg
    25021 ctgatcctgc tgcgcctgtc cggcaagcac acggtcggcg aattgagcat cttcgacctg
    25081 atcgtcgtga tcgtgatcgg cagtgcggtg cgcaccgccc tgctcggcaa cgacaagtcg
    25141 gtactcggcg gcctgctggt ggtgacggtg ttgctgttgc tggattacat cagcgcgttt
    25201 ttgtccgcgc gcttccgcaa ggcggagcat gtgctcgatg gcaagccggt gctgattgcc
    25261 cgcgacggcg tggtgttcca cgacgtgctg aagcaatgca acgttgcccc cggcaccttc
    25321 cagtcggcca tgcgaagcca cagcctgacc accgcgcaga tcggccaggc gatcctcgaa
    25381 gccaacggca acatcacctt ctgcaggaaa cccgaatgag gtgcggggct tgcgcggcga
    25441 tggtcacgcc cgcgccgcca cggcggcgcc gagcagcccg gggttggggt gcgtgatcac
    25501 ctgcaccgcc attcgctgca tgtaatcctc gtggcggccc ttcgccacga atgcttccat
    25561 gaattcgctg tcgcggaaga aatcgaggaa gtgcagcacg atgccgccgg cgatcag
//
LOCUS       DASSNH010000146         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_401274, whole genome shotgun sequence.
ACCESSION   DASSNH010000146 DASSNH010000000
VERSION     DASSNH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401274"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="ung"
                     /locus_tag="VFK00_05485"
     CDS             complement(<1..508)
                     /gene="ung"
                     /locus_tag="VFK00_05485"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7592439.1"
                     /translation="MTDERLKLEASWKARVGDYFDRPDMHALGDFLRAEKHNGRHVYP
                     PGAEIFAALDATPFDAVKVVILGQDPYHGPGQAHGLCFSVRPGVPPPPSLQNVFAEIE
                     SDLGIPRPDHGCLAPWAQRGVLLLNSVLTVRARMAGSHQGKGWEGFTDQVIEKLNRDR
                     EGLVFLLWG"
     gene            complement(759..1607)
                     /locus_tag="VFK00_05490"
     CDS             complement(759..1607)
                     /locus_tag="VFK00_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592440.1"
                     /translation="MNDVLRHLASDNPRVMVVDGSKVARTLIARVLEEDLPRATVITC
                     ARGTDAQRALQEGVVDLMTVALRLPDMDGSTLAKYVREHAPQAYIPIIAVSGDVEQRV
                     QNRQIGDEITDYFDKAAGYAALAEFIRGYVAPQDHAEGMVLYVEDSRVVAMATSRMLQ
                     RYGLTVHLAGSAEEAVEFLASARERGEAGADLVLTDVSLKGEMSGGDLLEWIRRDLQL
                     GKGQLPVLVMTGDGNPANQAALLKAGANDLVEKPVEERLLMTKLMFQLRVAQRLREQQ
                     QESVRQ"
     gene            complement(1745..2698)
                     /gene="ftsX"
                     /locus_tag="VFK00_05495"
     CDS             complement(1745..2698)
                     /gene="ftsX"
                     /locus_tag="VFK00_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET7592441.1"
                     /translation="MNARASMASDVRHTRFGMWRRQHAWCLRDSLRQLARRPLGTALT
                     IAVMGLALALPLAFYLLLANVQHLAGALGDSQAISVFLKSGQGAEQAATLAQGLRGRP
                     DVGSVAVRTPQQGLAELAAIQGFGDAIQSGPDNPLPFVLLVDPHPGTDRAQVEALVKD
                     VRAMPQVDLVQDNGQWRARLDALVELGRRIAVLLAVLLGAAAVLVIGNTVRLDIRGRA
                     EEIAVQRLIGASATFVRRPYLYEGALYGLAAGIIAVGLVLALEAVLAAPVRDLVASYA
                     GRLHFGGLSWGTLGATLGIALVLGWLGAWIASSRHLARTTA"
     gene            complement(2695..3423)
                     /gene="ftsE"
                     /locus_tag="VFK00_05500"
     CDS             complement(2695..3423)
                     /gene="ftsE"
                     /locus_tag="VFK00_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET7592442.1"
                     /translation="MHLSSCQARSSRSRRPSVIRFDSVSKRYPEGREALIDLSFVVEP
                     GEMVFVTGHSGAGKTTLLKLVAAIERPNHGTIEVHGRNLARLRRRDIPRLRRDIGMVF
                     QDHRLLMDRSVADNIALPLVIAGIPRDERARRVRGALEKVGLAGRGKDTPAALSAGEK
                     QRVGIARAIAGRPSLIIADEPTGNLDPQLSEEIMQLFTALKATGSTIVIASHDLPLLK
                     RMKQRVIVLDHGHLIDDVAAREVA"
     gene            complement(3596..4930)
                     /locus_tag="VFK00_05505"
                     /pseudo
     CDS             complement(3596..4930)
                     /locus_tag="VFK00_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008577362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            5173..5499
                     /gene="trxA"
                     /locus_tag="VFK00_05510"
     CDS             5173..5499
                     /gene="trxA"
                     /locus_tag="VFK00_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HET7592443.1"
                     /translation="MSENITHVKDSDFDAEVLQSETPVLLDFWAEWCGPCKSIAPMLE
                     DIAKEYAGRVKVVKINIDENQKTPMSYGIRGIPTLMMFKGGKVEATQIGAVGKGQLTQ
                     MIDKVV"
     gene            5685..>6554
                     /locus_tag="VFK00_05515"
     CDS             5685..>6554
                     /locus_tag="VFK00_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HET7592444.1"
                     /translation="MSDSENTVKGEDARASAPAPESNGSAGNDGGESQSQQRGNRPER
                     GRRRRGRHPRSGNGQGGNANGLPADVSGDEADAGDPDRLINLTELKRKNSVKLLELAE
                     ELGIQEGVARARKQDVIFNILRAHAKSGGSIWAEGVLEILQDGFGFLRSADESYLAGP
                     DDIYVSPSQIRRFNLRTGDYITGRVRHPKEGERYFALLKVDDINGDPPEASKNKLLFE
                     NLTPLFPRRAFHLERGNGSSEDITGRILDLIAPVGCGQRGLIVSQPKAGKTMMLQNVA
                     QALVHNHPEVHLII"
BASE COUNT         1095 a         2261 c         2109 g         1089 t
ORIGIN      
        1 tgccccacag caggaagacg aggccctccc ggtcgcggtt cagcttctcg atcacctggt
       61 cggtgaaacc ctcccaaccc ttcccctggt gcgaccccgc catgcgcgcc cgcaccgtca
      121 gcaccgagtt cagcagcagc acgccgcgtt gcgcccaggg cgcaaggcag ccgtggtcgg
      181 gccgcgggat gccgaggtcg ctctcgattt cggcgaacac gttctgcaac gacggcggcg
      241 gcggcacacc cggccgcacc gaaaaacaaa gtccgtgcgc ctggcccggg ccgtggtacg
      301 gatcctgccc gagtatcacc accttgaccg catcgaatgg cgtcgcatcg agcgccgcga
      361 agatttcggc ccccggtgga taaacgtgcc tgccgttgtg cttctcggcc cgaaggaaat
      421 ccccgagcgc gtgcatgtcg gggcgatcga agtaatcgcc cacccgcgct ttccacgacg
      481 cttcgagttt caggcgttcg tcggtcatgt cgccaatgcg gattcagcct gcaagtatac
      541 gccgtggcct tcccttctcc cttcgggagg aggtggcgcg cagcgccgga tgagggtccg
      601 ggcccgaaaa tggttgaacc attcgcgcat agttgagatt gcgcgagcgc gtactttcac
      661 ccgccccttc ggggcaccct ctcccggagg tagaggggaa taccgagacc gagccaggac
      721 aacggcggga accaacgatc gatcgccatc attttcgttc actggcgaac cgattcctgc
      781 tgttgctcgc gcaggcgctg cgccacccgc aactggaaca tcaacttggt catcagcagg
      841 cgctcctcga ccggtttttc cacgaggtcg ttggcgccgg ccttgagcag cgcggcctgg
      901 ttggcgggat tgccgtcgcc ggtcatcacc agcaccggca attgaccttt gccgagttga
      961 aggtcgcgcc ggatccattc caaaaggtcg ccgccggaca tttcgccctt caggctgacg
     1021 tcggtcaata ccaggtccgc accggcctcg ccacgttcgc gggcgctcgc caggaactcg
     1081 accgcctctt cggcactgcc ggcgaggtgc acggtcagcc cgtagcgctg caacatgcgg
     1141 ctggtggcca tcgcgaccac gcggctgtct tcgacataca gcaccatgcc ttccgcgtga
     1201 tcctgcggcg ccacgtagcc gcgaatgaac tcggccagcg ccgcgtagcc cgcggccttg
     1261 tcgaaatagt cggtgatctc gtcgccgatt tggcggttct gcacgcgttg ctcgacgtca
     1321 ccggacaccg cgatgatcgg gatgtaggcc tgtggcgcgt gttcgcgcac gtacttcgcc
     1381 aacgtcgatc catccatgtc cggcaagcgc aacgcgaccg tcatgaggtc gacgacgccc
     1441 tcctgcaacg cccgctgcgc atcggtcccg cgcgcgcaag tgatcaccgt cgcccgcggc
     1501 aggtcctcct ccagcacccg tgcgatcagc gtgcgcgcca ccttggagcc atccaccacc
     1561 atcacccggg gattgtcgct tgcaaggtgg cggaggacgt cgttcatgct tcggtcgagg
     1621 tacgttggcg aggccgggga aaatccgagt cggagtgcac tcgttggccc ctcggcgttg
     1681 cttgaaactg tagttcgagt gcgctctgac cccggttttc ccgaccccgg tttttccccg
     1741 gttttcaggc cgtggtgcgc gccaggtgcc tgctgctcgc gatccatgcc cccagccaac
     1801 cgagcaccag cgcgatgccg agcgtggccc cgagcgtacc ccatgacaag ccgccgaagt
     1861 gcaggcgccc cgcataactg gcgaccaggt cacgtaccgg cgcagccagc accgcttcca
     1921 gggcaagcac cagccccacc gcaatgattc cggccgcgag cccgtagagc gccccctcgt
     1981 acaggtacgg tcggcgcacg aaggtggcgc tggcaccgat caggcgctgc accgcgattt
     2041 cctccgcccg cccgcggatg tcgagccgca cggtattccc gatcaccagt accgcggcgg
     2101 cgcccagcag caccgccagc agcaccgcga tgcgtcgccc gagttcgacc agcgcatcca
     2161 gccgcgcccg ccattgccca ttgtcctgga ccaggtcgac ctgcggcatc gcgcgcacgt
     2221 ccttcaccag ggcttcgacc tgcgcgcgat ccgtgcccgg atgcggatcg accagcaaca
     2281 cgaacggcag ggggttgtcc ggccccgact ggatagcgtc gccgaacccc tggatcgcgg
     2341 cgagctccgc caatccctgt tgcggggtgc gtaccgccac ggaaccgaca tcggggcggc
     2401 cgcgcaagcc ctgggccaag gtggcagcct gttcggcgcc ctggcccgac ttcaggaata
     2461 cgctgattgc ctgggagtcg cccaaggcgc cggcgaggtg ctggacattg gccagcagca
     2521 ggtagaacgc caacggcagc gccagcgcaa ggcccatgac cgcgatcgtc agcgcggttc
     2581 cgagcggccg ccgcgccaat tgcctcaaac tgtcgcgcag gcaccacgcg tgttgccgcc
     2641 gccacatgcc gaagcgcgtg tggcgcacgt ccgaagccat cgacgcgcgc gcgttcatgc
     2701 gacctcccgc gcagccacgt cgtcgatcag gtgcccatgg tccagcacga tcacgcgctg
     2761 cttcatccgc ttcagcagcg gcaggtcgtg gctcgcgatg acgatggtgc tgccggttgc
     2821 cttcagggcc gtgaacaact gcatgatctc ctcggacaat tgcggatcga gattgccggt
     2881 cggctcgtcg gcgatgatca gcgacggccg gccggcgatc gcgcgcgcaa tgcccacccg
     2941 ctgcttctcg ccggcggaaa gcgccgccgg cgtatccttg ccgcggcccg cgagtccgac
     3001 cttctccagt gcgccgcgca cccgcctggc tcgctcgtcg cgcggaatgc ccgcgatgac
     3061 caacggcagc gcgatgttgt cggcgacgct ccggtccatc agcaggcgat ggtcctggaa
     3121 caccatgccg atgtcgcgtc gcaggcgcgg gatgtcgcgt cgccggagtc tggcgagatt
     3181 gcggccatgc acctcgatcg tgccgtggtt cggccgttcg atcgccgcga cgagcttcaa
     3241 caacgtggtc ttgccggcgc ccgaatggcc ggtcacgaag accatttcgc cgggctcgac
     3301 gacgaagctg aggtcgatca acgcctcgcg accttcgggg tagcgtttgc tgacggaatc
     3361 gaagcggatc acggacggtc gtcgactacg agatgatcgg gcctggcaag aggataagtg
     3421 catttacccc cttccgaagg agggggtcgc cgcgaagcgg cgggggatgt aacggtgcag
     3481 tcgtcaccag gatcaaaagc catccccctc gatccccctt cgcgcgcgaa gggggaagaa
     3541 aatgcatggg actcgccgct tcttccgccc tttgcaggcg aaggggaaga cgtcaaccgc
     3601 ctgctgcggg cgcagcggtg cgcgccgcgc ccgacttgcg gcggcgccgg cgcttggcgg
     3661 gcgcggcctt gccggcgccg gccggggccg cggcttcgac cgcgccgggt tcgccttcgc
     3721 catgatgggc cactccggcg tgtgcacgcg gcgccggcgc ggccagccaa tccggttcga
     3781 tcgcctgcac cggaatcttc attccgatgt agtgctcgat gtcgggcagg ttcatcgcgt
     3841 acaggtcgca ggcgaagctg atcgcgtcgc cttcggcgcc cagtcgcgcg gtgcgaccga
     3901 tgcggtgcac gtagtcctcg ccgtcctgcg gcaggtcgta gttgaagacg tgggtgacat
     3961 cggggatgtg cagtccgcgc gccgcgacgt ccgtcgccac aaggaggtcg atttcaccct
     4021 tcttgaaacg ctccatcagt cgctggcgct tggcctgcgg cacgtcgccg gacaacgcgc
     4081 ccacgcgaaa accctgccgc gaaaggcgct gcgtcacccg ttcggcagcg gcgcgcgtat
     4141 tcacgaagac gatgctgcgc tgctggccgc tgttctccag caggttcagg agcaacggga
     4201 tcttttcttc cttggccggg aagaacacct gctggcgcac gcggtcggcg gtgacgtgtt
     4261 cggtttcgac gctcagcttc tcgggctggt tcatgtgctc gtaggcgagt tccagcacgc
     4321 gataggagag cgtggccgag aacagcatgc cctggcgttc ctcgcgcggc ggcatcttgc
     4381 gcaacaggta gcgcacgtcc ttgatgaagc cgaggtcgaa catccggtcc gcctcgtcga
     4441 tcacggttgc ctcgaccgcg cggaaactga agacgtgctg cttgaagtag tcgatcagcc
     4501 ggccgggcgt cgcaatgatg acgtcgcagc cgtcgcgcag ttgctgacgc tgcttgtcat
     4561 aatccacgcc gccgtagatc agcgcgaacc gcagcccggt gtggcgcccg atcgcctgcg
     4621 catccttgtc gatctggatc gcgagttcgc gggtcggcgc cacgatcacg gcccgcggat
     4681 cgttgtcctt gcgctcgggg atcgcggccc gggtgagcag gcggttcatc acggcgacca
     4741 ggaaggcgca ggtcttgccg gtgccggtct gggcctggcc cgcgacgtcg cgccccgcca
     4801 gcgccaccgg cagggtcagc gcctggatcg gcgtgcagcg ggtgaacccg caatcggcga
     4861 ggccggcagc gaggtggggg tgcagttcga actgttcgaa aaaagtgtcg gtgagaactt
     4921 ggtcggccac gtggtttatc agtcggaaag gggtgccgcc gttgccggca gtcggcgtgg
     4981 gagccggtgc cgcatttggt ccaggcgccg ggttgcggtg gaatgcgctc ggcgtggcat
     5041 ggcatttcgc gaggttccgc acgcgaaatg cactggcgga gcacctatag tagcagggtg
     5101 ccggcttccg ccgccttgca atgcggcggt cggccccaag attgtccccc gtcccccacc
     5161 ccaggtcttt caatgagcga aaacatcacc cacgtcaagg actccgattt cgacgccgaa
     5221 gtgctgcaat ccgaaactcc ggtgctgctg gatttctggg ccgaatggtg cgggccgtgc
     5281 aagtcgatcg caccgatgct cgaggacatc gccaaggaat acgccgggcg ggtcaaggtc
     5341 gtgaagatca acatcgacga gaaccagaag accccgatgt cctacggcat ccgcggcatc
     5401 ccgaccctga tgatgttcaa gggcggcaag gtcgaggcca cccagatcgg cgcggtcggc
     5461 aagggccaac tgacccagat gatcgacaag gtcgtttgaa tcgcagggcg gacaccgtcc
     5521 gcccccgggg ggtgggcaat gcccgccccg cctgaacctt ctcccgcgcg gcgcttgtgc
     5581 accggtgccg cgcaatgcta aattgatccc cggctggtgg tgcttctgcc cagcctccct
     5641 tcttttttct cctcccgcgc cccggcgccg acgaggctta tcctgtgtca gatagcgaaa
     5701 acaccgtaaa gggcgaagac gcccgcgcgt ccgcccctgc tcccgagtcg aacggctccg
     5761 ccggcaacga tggtggcgaa agccagtcgc agcagcgtgg caaccgcccc gagcgtggtc
     5821 gccgccgccg cggccgccac ccgcgtagcg gcaacggcca gggcggcaac gccaacggcc
     5881 tgcccgcgga cgtcagcggc gacgaagccg acgccggcga tcccgatcgc ctgatcaacc
     5941 tcaccgagct gaaacgaaag aattcggtga agttgctgga actggccgag gaactcggca
     6001 tccaggaagg cgtcgcccgc gcccgcaagc aggacgtgat cttcaacatc ctgcgtgcac
     6061 acgccaagtc cggcggcagc atctgggccg agggcgtgct cgaaatcctg caggacggct
     6121 tcggcttcct gcgctcggcc gacgagtcct accttgccgg tcccgacgac atctacgtct
     6181 cgccctccca gatccgccgc ttcaacctgc gcaccggtga ctacatcacc ggccgcgtcc
     6241 gccatccgaa ggaaggcgag cgctacttcg cgctgctcaa ggtcgacgac atcaatggcg
     6301 atccaccgga ggcctccaag aacaagctgc tgttcgagaa cctgacgccg ctgttcccgc
     6361 gccgggcatt tcacctggag cgcggcaacg gctcgtccga agacatcacc ggacgcatcc
     6421 tcgacctgat cgcgccggtc ggctgcggcc agcgcggcct catcgtcagc cagcccaagg
     6481 ccggcaagac catgatgctg cagaacgtcg cgcaggcgct ggtgcacaac caccccgagg
     6541 tgcacctgat catc
//
LOCUS       DASSNH010000147         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1433409, whole genome shotgun sequence.
ACCESSION   DASSNH010000147 DASSNH010000000
VERSION     DASSNH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433409"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFK00_05520"
     CDS             <1..589
                     /locus_tag="VFK00_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592445.1"
                     /translation="PALINASFPPWFAGFCFAAIALGALVPAAIMSIGAANLFTRNIW
                     RPYVNEQLSHAQESRIAKNISLLVKFGALLAVLYLPTKFAIDLQLLGGIWMLQIFPAV
                     ICGLYTRWLRASALLLGWFAGMVLGSALAWQQGLKPVYTFVFGDAHYGIYIGLLALAL
                     NFVVSILATPVAGRLRREGPPDRTNPLDYQDVVPD"
     gene            complement(803..>2575)
                     /locus_tag="VFK00_05525"
     CDS             complement(803..>2575)
                     /locus_tag="VFK00_05525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7592446.1"
                     /translation="GTPHSVSPSIVGMDSPPNKLKYRCHWTPPVAIDPFDHDSVLYGC
                     QMLLRTTDKGHSWTEFSPDLSTRDPSLIVPSGGIMQDNLGQYFGALVWSLAYSPIEKG
                     LLWAGTNDGKVWYTNDAESQGQPQWIDVTANLHIPPLGNINQIAPSHFHAGTAYIAVD
                     FRFAGEGNHKPYILETADYGKTWKNISSNLPSDNPLDYTLSVAENPNREGMLFAGTGH
                     AFYYTMDNGRHWTRFRKGLPPSPVTWINIQPHMHDVDVSTYGRGDFILPNIATFEQTG
                     SPGQPGSGATRLFKPGVVYRQARSAYPTAAQPARPQFQFYLAKAPQGPVQLQILDAQG
                     KAIRTEKLQAHQGLNGAWWDLFYDTPSDVRLLTTPPENPHIWDEPRYQGKTYRSIIHW
                     GANPHTGTPIAAPGHYQVRLTAEGRSYTQPFEVVKDPAVKASDAVLADSTAMQVQIAD
                     AISETSDMVNTMEKWRKQVEDQLETHASGDAADALRQLDGQILDVEDQLVSPEARLSD
                     DKQYSTPFRMYWNLLWLAGRVGQGIQNTAGGSDYEPTVAQRENFAKLQAELAATRTAF
                     ESLKTTTVPAFNQGGHGVKIEVGE"
BASE COUNT          407 a          813 c          831 g          524 t
ORIGIN      
        1 gcccgcgctg atcaacgcga gcttcccgcc gtggttcgcc gggttctgct tcgccgcgat
       61 cgcactgggc gcgctggtgc cggcggcgat catgtcgatc ggcgccgcga accttttcac
      121 gcgcaacatc tggcggccgt atgtcaacga acagttgtcg cacgcgcagg aatcgcgcat
      181 cgcaaagaac atctccctgc tggtgaagtt cggcgcgctg ctggcggtgc tgtacctgcc
      241 gacgaagttc gcgatcgacc tgcaactgct tggcggcatc tggatgctgc agatcttccc
      301 cgcggtgatc tgcggcctgt acacacgctg gctgcgcgca tcggccctgc tgctcggctg
      361 gttcgccggc atggtgctcg gcagcgcgct cgcgtggcag cagggattga agccggtgta
      421 cacgttcgtg ttcggcgacg cgcactacgg catctacatc ggcctgctgg cgctcgcgtt
      481 gaacttcgtg gtttccatcc tcgccacacc cgtcgcgggg agactcaggc gcgaaggccc
      541 tccggaccga acgaatcccc tcgattacca agacgtcgtg ccggattgac cacggacggc
      601 ttcggcgctc ttgtaggttg gcgctgagcg cagcgaagcc caacgcgggg cgcgagttgt
      661 ccccaatcgc ggttgtcgcg tttcggtctc caacgttggg cttctcccgg aacaggttcc
      721 gggatcaacc caacctacaa aagctggcgg cttcggcgct tttgtaggtt ggcgctgagc
      781 gcagcgaagc ccaacgcggc cgctactcgc ccacctcgat cttgacaccg tggccgccct
      841 ggttgaacgc gggaacggtc gtggtcttca aactttcgaa ggccgtccgg gtggctgcga
      901 gttcggcctg gagctttgcg aagttttcgc gttgggcgac agtgggctcg tagtccgaac
      961 cgccggcagt gttctggatg ccctgcccga cgcggcccgc cagccagagc aggttccagt
     1021 acatccggaa cggagtcgag tattgcttgt cgtcgctgag cctcgcttcc ggactgacca
     1081 attggtcctc gacatccaga atctggccat ccaactgtcg aagcgcatcc gcggcgtcac
     1141 ccgaggcgtg cgtctccaat tgatcctcga cctgcttgcg ccacttctcc atggtattga
     1201 ccatgtccga ggtttcgctg attgcgtcgg cgatctgcac ttgcatcgcg gtcgaatcgg
     1261 ccaagacggc atcactcgcc ttgaccgccg ggtccttcac gacctcgaag ggctgcgtgt
     1321 agctgcgacc ctcggcggtc agccgcacct ggtaatggcc gggtgccgcg atcggcgtcc
     1381 cggtatgcgg gttggcgccc cagtggatga tgctgcggta tgtcttgcct tgatagcgcg
     1441 gctcgtccca gatgtgcggg ttttcgggtg gtgtcgtgag cagcctgaca tcgctcggcg
     1501 tgtcgtagaa aagatcccac caggcgccat taaggccttg gtgagcctga agtttttcgg
     1561 tgcgaatggc cttcccctgc gcatccagga tctgcaactg cacggggccc tggggcgcct
     1621 tcgccaggta gaactggaac tgcggccgcg ccggctgggc cgcggtcgga tacgcactgc
     1681 gcgcctgccg atagaccacg ccgggcttga acagccgcgt cgctccactg cccggttgcc
     1741 ccgggctccc ggtctgctcg aacgtcgcga tgttcggcag gatgaaatcg ccgcggccgt
     1801 aggtggacac gtccacgtcg tgcatgtgcg gctggatgtt gatccaggtg accggcgacg
     1861 gcggcaagcc tttgcggaat cgcgtccaat gccgaccgtt gtccatcgtg taatagaacg
     1921 catggccggt gcccgcgaac agcatgccct cgcggttggg gttttccgcc acgctcagcg
     1981 tgtaatcgag cggattgtcg gaaggaagat tgctgctgat gttcttccag gtcttgccgt
     2041 aatcggcggt ctcgaggatg tacggcttgt ggttgccttc acccgcgaaa cggaaatcca
     2101 ccgcgatgta ggcggtgcca gcatgaaagt gcgacggtgc gatctggttg atgttcccca
     2161 gcggcggaat gtgcaggttc gcggtgacgt cgatccactg cggctgccct tgcgactcgg
     2221 catcattcgt gtaccagacc ttgccgtcat tggtgccggc ccacagcagg cccttctcga
     2281 tcggcgagta ggccagcgac cacaccagtg cgccgaagta ctggccgagg ttgtcctgca
     2341 tgatgccgcc gctcggaacg atcagcgacg gatcgcgcgt ggacaggtcc gggctgaact
     2401 ccgtccacga atgtcccttg tccgtcgtgc gcagcaacat ctggcagccg tacagcacgc
     2461 tgtcgtggtc gaacggatcg atagcgaccg gcggcgtcca gtggcagcgg tacttcagct
     2521 tgttcggcgg cgaatccatg ccgacgatcg acggcgacac cgaatgcggc gtgcc
//
LOCUS       DASSNH010000148        11763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1137665, whole genome shotgun sequence.
ACCESSION   DASSNH010000148 DASSNH010000000
VERSION     DASSNH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137665"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFK00_05530"
     CDS             complement(<1..183)
                     /locus_tag="VFK00_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7592447.1"
                     /translation="MIGFRDFALRRGGRLLLAGVDAVLYDGWKVGVAGRNGTGKSSLF
                     AAILGEIEADAGALDVP"
     gene            complement(180..656)
                     /gene="greA"
                     /locus_tag="VFK00_05535"
     CDS             complement(180..656)
                     /gene="greA"
                     /locus_tag="VFK00_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7592448.1"
                     /translation="MQRAPMTKAGSERLRAELDRLKSQERPRIIAAIAEARAHGDLSE
                     NAEYNAAREQQSFVEGRIHAIEAALSTAQIIDPATLNPGEKVVFGAVVDLEDADNGEA
                     VTYQIVGDIEADIKKGLIAVSSPIARAMIGKCEGDSFTFEAPAGERNYEITGVRYA"
     gene            complement(737..4006)
                     /gene="carB"
                     /locus_tag="VFK00_05540"
     CDS             complement(737..4006)
                     /gene="carB"
                     /locus_tag="VFK00_05540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7592449.1"
                     /translation="MPRRTDIKTILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     VLVNSNPATIMTDPEMADRVYIEPINWRTVERIIEKERPDALLPTMGGQTALNCALEL
                     ADHGVLEKHGVELIGASRDAIRMAEDRELFRNAMDEIGLASPKSAIARTLDQAREGQA
                     ALGFPCIVRPSFTLGGTGGGIAYNVEEFEKIVLRGLELSPTHEVLIDESVLGWKEFEM
                     EVVRDRADNCIIVCAIENFDPMGVHTGDSITVAPALTLTDKEYQRLRDYSIAVLRKIG
                     VDTGGSNVQFGVNPADGRVVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNEITGGRTPASFEPALDYIVTKIPRFAFEKFPAADPHLTTQMKSVGEVMAIGRTFP
                     ESLQKALRGLETGHDGLNPGGIDWHTPEGMELLKRELRQPGPERIFHVGDAFRAGLSL
                     EDVHGLSKIDPWFLAAIEDIVATEADVRAGGMRALDAGRMRELKRLGFSDSRLAKLLQ
                     SDEGSVRHVRHTLGVRPVYKRVDSCAAEFATTTAYMYSTYEEECEANPTDRKKIMVLG
                     GGPNRIGQGIEFDYCCVHAALAMREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVDKEKPTGVIVQYGGQTPLKLARALEAAGVPIIGTSSDSIDLAEDRERFQQLI
                     EKLKLKQPPNRIARTAEEAMVLARELGYPLIVRPSYVLGGRAMEVVHADEDLARYIRD
                     AVRVSESSPVLLDRFLDNAVEVDVDAIADADGTVLIGGIMQHIEEAGVHSGDSSCSLP
                     PYSLKSGIQDELRRQVALLARELGVVGLMNTQFAIQGEDVYILEVNPRASRTVPFVSK
                     ATGRPLAKIAARCMSGKSLAEQGATRERVPHYWSVKEAIFPFLKFQNVDPILGPEMRS
                     TGEVMGVGRSFGEAFARGHEAAHVAAPAAGSKVFLSVRDADKSRLLEVARDLRERGFS
                     LIATGGTAAYLREHGIECERINKVLEGRPHIVDAIKNGEISYIVNTTEGRQAIADSFS
                     IRRSALQQHVTYSTTVAGAAALLHSLDFREQGGVTSLQELHAELDERDARGPDRDAAA
                     A"
     gene            complement(4006..5133)
                     /gene="carA"
                     /locus_tag="VFK00_05545"
     CDS             complement(4006..5133)
                     /gene="carA"
                     /locus_tag="VFK00_05545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7592450.1"
                     /translation="MPSRAMLALADGTLFHGDAIGAMGATVGEVVFNTSMTGYQEILT
                     DASYAQQIVTLTVPHVGITGCNAVDEESGKVQAAGLIVRDVPRLPSNWRSTESLPNYL
                     RRNGIVAISGIDTRKLTRILRDRGAQHGCIVAGDSIDADDALARARAFPGLNGADLAK
                     VVSLREPAPWNEGNYELDDQAFRKPAAKLKVVAYDFGLKRNILRLLVEQGCDITVVPA
                     QTPAEQVMAMQPDGVCLTNGPGDPAACTYAIDATRVLLDAKVPLLGICLGHQLMGLAL
                     GAKTLKMPFGHHGANHPVKDLDDGRVLITSQNHGFAVDPATLPSNTRVTHTSLFDGSL
                     QGFALTDRPAFCFQGHPEASPGPHDIAYLFQRFANVMQEAR"
     gene            5291..5986
                     /locus_tag="VFK00_05550"
     CDS             5291..5986
                     /locus_tag="VFK00_05550"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET7592451.1"
                     /translation="MPSPALLDPARLDRPDTLVQRHPFSFLIAHGQLPDASRADLGRD
                     FPKYSGAGFFPHDARECGDSINELVEAITAPEFASAVGAKLGIDDLGQYPTLVTICRH
                     LNKRHGTIHTDSKSKVATALLYLNSSWNDTSDGCLRFLQSVDDIDATIAPEVKPLYGE
                     FVIFKRADNSFHGHLPFEGERRVIQVAWLTSEEEKLRKTQRGTATRFFKKLAGRLDRK
                     FGAGRDRSAAHRN"
     gene            complement(6074..6814)
                     /gene="dapB"
                     /locus_tag="VFK00_05555"
     CDS             complement(6074..6814)
                     /gene="dapB"
                     /locus_tag="VFK00_05555"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7592452.1"
                     /translation="MSSPIRLAINGADGRMGRVLQALVREDGRFELAHRVNASSGWTN
                     APKLDAMIDFSTPAGFDAALAHCREHRIVFVSGTTGLDATRQAALDEAAREIPALHSA
                     NFSLGIAVLTRALRDAAAALPDWDLEILEAHHVRKTDAPSGTALALGLAAASARGQDF
                     ESVAVLSREGQGGSRPAGSIGFAAIRAGDIVGEHTAILATAGERIELSHRATDRGIFA
                     RGALAAAAWLATKPPGMYSIGDVIGSRG"
     gene            complement(7036..8154)
                     /gene="dnaJ"
                     /locus_tag="VFK00_05560"
     CDS             complement(7036..8154)
                     /gene="dnaJ"
                     /locus_tag="VFK00_05560"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7592453.1"
                     /translation="MKRCYYEILGVERTANDEELKKAFRRLAMKNHPDRCPDDPTAQE
                     RFKEAKEAWEILGDPERRALYDQYGHAAFENGAGRRRGGAPFGDVGDIFGDIFSDIFG
                     GGRRGARGSDLRYVLELDLEEAVAGIEKQIRVPTMVNCHHCNGTGSEDGKLSTCATCR
                     GHGRVRMQNGIFSIQQTCPHCGGAGRMVVTPCKHCHGEGRLEDERSLEVKVPAGVDDG
                     DRIRLTGQGEAGAAGTTPGDLYVEIHVREHPVFRREGDNLRCEVPVRFAQAALGATIE
                     VPVLGGATTITVPPETQTGQEFRLRGQGVKSVRSRHTGDLYCTVVVETPVRLDKRQRE
                     LLEEFEATFADAEEAKKHSPRSSGFVDNVKQFWSRMTG"
     gene            complement(8151..8735)
                     /locus_tag="VFK00_05565"
     CDS             complement(8151..8735)
                     /locus_tag="VFK00_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET7592454.1"
                     /translation="MHKGRMEAFTDGVIAVIITVMVLELKLPREATWQALLAGLPVFL
                     SYVLSFAYVGIYWNNHHHMLQMVTHVDGKVLWANLFFLFWLSLFPFTTSWLNESHPVP
                     AAVPTALYGVVLLLAALSWVPLLRSLIAANGGREGALASALRHDWKVIVSPIGYVTGI
                     ALAFFEPILSCVIYGVVAALWFVPDRRLEARLRA"
     gene            complement(8849..10780)
                     /gene="dnaK"
                     /locus_tag="VFK00_05570"
     CDS             complement(8849..10780)
                     /gene="dnaK"
                     /locus_tag="VFK00_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7592455.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGTKTRVIENSEGDRTTPSIVAFSK
                     DGEVLVGAPAKRQAVTNPKNTFYAVKRLIGRKFTDAEVQKDLDVVPYKITQHSNGDAW
                     LETSDGKQMAPPEISAKVLMKMKKTAEDFLGETITDAVITVPAYFNDSQRQATKDAGK
                     IAGLEVKRIINEPTAAALAYGLDKGGGDRKIAVYDLGGGTFDVSLIEIAEVDGEKQFE
                     VLATNGDTFLGGEDFDKRVIDYLVDEFKKDQGVDLRNDPLALQRLKDAAERAKIELSS
                     AHQTEVNLPYVTADASGPKHLNIKLTRAKLEALVEDLVKRTIEPCRTALNDANLRVSD
                     ISEVILVGGQTRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGGVLSGDVKDVLL
                     LDVTPLSLGIETLGGVMTKLIDKNTTIPTKASQVFSTAEDNQSAVTVHVLQGERDQAR
                     FNKSLAKFDLAGIQAAPRGMPQIEVSFDIDANGILHVTAKDKNTGKEQRIEIKAGSGL
                     SEEEIQQMVSDAETHREEDKKFQELVVTRNKADQLVHATRSAIKEHGGKVGGDVIGRV
                     EESLSELEKVMKGDDKDAIESRISKLEEAAQSLFAAAQAGGAQPGPQPGAQQAPGGGS
                     ASPEDVVDAEFTEVKDKDK"
     gene            complement(10969..11517)
                     /gene="grpE"
                     /locus_tag="VFK00_05575"
     CDS             complement(10969..11517)
                     /gene="grpE"
                     /locus_tag="VFK00_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7592456.1"
                     /translation="MNPNESRGSPGGDPQDDRPADPAGAPDGDELETLSRQLAEAETS
                     LAGMRETLLRERADLENQRKRMQRDLEQSLKFANEKLLRDLLPVYDGLESGLAVETAD
                     VASMREGLSLTLKSLLKIAESNGLEQVDPVGEPLDPERHHAVSMVEASGARPGTVVGV
                     MQKGYVLNGRLLRPALVTVAKE"
BASE COUNT         1894 a         3947 c         3768 g         2154 t
ORIGIN      
        1 gggcacgtcc agggccccgg catccgcctc gatctcgccg aggatcgccg caaacaggct
       61 ggacttgccg gtgccattgc gcccggccac gccgaccttc catccgtcgt acagcaccgc
      121 atcgacgccc gccaacagca gccgtccacc gcggcgaagg gcgaagtcac ggaagccgat
      181 cacgcgtatc gcacgcccgt gatttcgtag ttgcgctcgc ccgccggcgc ctcgaaggtg
      241 aagctgtcgc cttcgcactt gccgatcatc gcgcgcgcga tcggcgagga caccgcgatc
      301 aggcccttct tgatgtcggc ttcgatgtca cccacgatct ggtaggtgac cgcctcgcca
      361 ttgtcggcgt cttcgaggtc caccaccgcg ccgaacacca ctttctcgcc cgggttgagg
      421 gtggccgggt cgatgatttg cgcggtcgaa agcgcggcct cgatcgcgtg gatgcgccct
      481 tcgacgaagc tttgctgctc gcgcgcggcg ttgtattcgg cgttctcgct taggtcgccg
      541 tgggcgcgag cctcggcgat cgcggcgatg atccgcgggc gttcctggga tttcagtcga
      601 tccagttctg cgcgcagacg ctcggaaccg gctttggtca tgggggctcg ctgcatgtgc
      661 gggactcctt ggaattgcgt gcctcgcatc cgccgggacg cgcgggcatg ggtggatgaa
      721 tgaaccgggg cgatactcag gccgcggcgg catcccggtc gggcccgcga gcgtcgcgct
      781 cgtccagctc ggcgtgcagt tcctgcaagc tggtgacgcc gccctgctcg cggaaatcca
      841 gcgagtgcag cagcgccgcc gcacccgcga cggtggtcga ataggtgacg tgctgctgga
      901 gcgcggagcg ccggatcgag aacgagtcgg cgatcgcctg tcgcccttcg gtggtgttga
      961 cgatgtagga aatctcgccg ttcttgatgg catcgacgat atgcggccgg ccttccagta
     1021 ccttgttgat gcgttcgcac tcgatgccgt gctcgcgcag gtacgccgcg gtgccgccgg
     1081 tcgcgatcag ggagaagccg cgctcccgca ggtcgcgcgc cacctccagc agccgggact
     1141 tgtcggcgtc gcgaaccgac aggaatacct tgctgcccgc cgccggcgcc gcgacgtggg
     1201 cggcctcgtg gccacgcgcg aacgcttcgc cgaagctgcg cccgacgccc atcacctcac
     1261 ccgtcgaacg catctcgggc ccgaggatcg gatcgacatt ctggaacttg aggaacggga
     1321 agatcgcttc cttgaccgac cagtagtgcg gcacccgctc ccgcgtggcg ccctgttccg
     1381 cgaggctctt gccggacatg cagcgcgcgg cgatcttggc cagcggacgg ccggtggcct
     1441 tggacacgaa cggcaccgtg cgggacgcgc gcgggttgac ttcgaggatg tagacgtcct
     1501 cgccctggat cgcgaactgg gtgttcatca gccccaccac gccgagctcg cgtgccagca
     1561 acgccacctg ccgcctcaat tcgtcctgga tcccggactt cagcgaatat ggtggcagcg
     1621 agcacgagga atcgcccgag tgcacgccgg cttcctcgat gtgctgcatg atgccgccga
     1681 tcagcaccgt gccgtcggcg tcggcgatcg catcgacgtc gacctcgacc gcgttgtcca
     1741 ggaagcggtc gagcagcacc ggcgacgatt ccgacacgcg caccgcgtcg cggatgtaac
     1801 gggcgaggtc ctcgtccgcg tgcaccactt ccatcgcccg gccgcccagc acgtagctcg
     1861 gccgtacgat cagcggatag ccgagctcgc gtgccagcac catggcctcc tcggcggtgc
     1921 gcgcgatccg gttgggaggc tgcttcagct tgagcttctc gatcaactgc tggaaacgct
     1981 cgcgatcctc ggcaagatcg atcgagtccg acgaggtgcc gatgatcggg acgcccgcgg
     2041 cctcgagcgc gcgcgccagc ttcagcggcg tctgcccgcc gtactgcacg atcacgccgg
     2101 tcggcttctc cttgtcgacg atttccagca cgtcttccag cgtcaccggc tcgaaataca
     2161 gtcgatccga tgtgtcgtaa tcggtggaca cggtttccgg attgcagttg accatgatgg
     2221 tctcgaaccc gtcctcgcgc atcgcgagcg cggcgtgcac gcagcagtag tcgaactcga
     2281 tgccctgccc gatccggttg gggccgccgc ccagcaccat gatcttcttg cggtccgtcg
     2341 ggttggcctc gcactcttcc tcgtaggtcg aatacatgta cgccgtggtg gtggcgaatt
     2401 cggccgcgca ggaatcgacg cgcttgtaca ccggtcgcac cccgagcgtg tggcgcacgt
     2461 ggcggaccga gccctcgtcc gactgcagca gcttggcgag ccgcgaatcg gagaacccga
     2521 ggcgcttcag ttcacgcatg cggcccgcgt ccagcgcgcg catgccgccg gcgcgcacgt
     2581 cggcctcggt cgcgacgatg tcctcgatcg cggccaggaa ccacggatcg atcttcgaca
     2641 acccatggac atcctcgagc gacaagcccg cgcggaaggc gtcgcccacg tggaagatcc
     2701 gctcgggtcc gggctggcgc aactcgcgct tcagcagttc catgccctcc ggcgtatgcc
     2761 agtcgatgcc gccgggattg aggccatcgt ggcccgtctc gaggccgcgc agcgccttct
     2821 gcagcgactc gggaaacgta cggccgatcg ccatcacctc gcccaccgat ttcatctggg
     2881 tggtcaaatg cgggtcggcc gccgggaact tctcgaacgc gaagcgcggg atcttggtga
     2941 cgatgtagtc gagcgccggc tcgaacgacg ccggggtcct gccgccggtg atttcgttcc
     3001 tgagttcatc gagcgtgtag ccgaccgcca gcttggccgc gaccttggcg atcggaaagc
     3061 ccgtcgcctt ggaggccagc gccgaggacc gcgacacccg cgggttcatc tcgatcacga
     3121 cgacccggcc atcggcgggg ttcacgccga actgcacgtt gctgccaccg gtgtcgacgc
     3181 cgatcttgcg cagcaccgcg atcgagtaat cacgcaggcg ctggtattcc ttgtcggtaa
     3241 gggtgagcgc cggcgccacc gtgatcgagt cgccggtgtg cacgcccatc gggtcgaagt
     3301 tctcgatggc gcacacgatg atgcagttgt ccgcacggtc gcgaacgact tccatctcga
     3361 attccttcca gcccagcacc gactcgtcga tcaacacctc gtgcgtcggc gacaactcca
     3421 gcccgcgcaa aacgatcttc tcgaattcct cgacgttgta ggcgatgccg ccgccggtgc
     3481 cgcccagcgt gaatgacggg cgtacgatgc agggaaatcc gagcgcggcc tgcccttcgc
     3541 gcgcctggtc cagcgtccgc gcgatcgcgg acttcggcga ggcgagcccg atctcgtcca
     3601 ttgcgttgcg gaacaactcg cgatcctcgg ccattcggat cgcgtcccgc gacgcgccga
     3661 tcagttcgac accgtgtttt tccagcacac catggtcggc gagttccagt gcgcagttga
     3721 gcgcggtctg gccgcccatg gtcggcagca gcgcgtcggg cctttccttc tcgatgatgc
     3781 gctcgacggt gcgccagttg ataggctcga tgtagacccg atcggccatc tcggggtcgg
     3841 tcatgatcgt ggccgggttc gaattcacca gcactacccg gtagccttcc tcacgcagcg
     3901 ccttgcaggc ctgcgcgccc gagtagtcga actcgcaagc ctggccgatc acgatcggac
     3961 cggcaccgat gatgaggatg gttttgatgt ctgtgcggcg gggcatcagc gagcctcctg
     4021 catcacattc gcgaatcgct ggaacaggta cgcaatatcg tgcggcccag gactcgcctc
     4081 cgggtgtccc tggaagcaga acgccgggcg atctgtgagc gcgaagccct gcagcgaacc
     4141 gtcgaaaagc gaggtgtggg tgacccgcgt gttcgacggc aacgtcgcgg ggtcgacggc
     4201 gaaaccgtgg ttctgcgagg tgatcagcac gcggccgtcg tcgaggtcct ttaccgggtg
     4261 gttggcgccg tggtggccga acggcatctt cagcgtcttc gcgccgagcg ccaggcccat
     4321 caactggtgg cccaggcaga ttcccaacag cggaaccttg gcgtccagca agacgcgggt
     4381 tgcgtcaatc gcataagtgc aggcggcagg atcgccgggt ccgttggtga ggcagacacc
     4441 gtcgggctgc atcgccatga cttgctcggc cggtgtctgc gcgggcacca ccgtgatgtc
     4501 gcatccttgc tcgaccagca ggcgcaggat gttgcgcttg agcccgaaat cgtaggcgac
     4561 caccttgagc ttcgcggccg gtttgcggaa cgcctggtcg tccagttcgt agttcccttc
     4621 gttccatggt gccggttcgc gcaggctgac caccttggca aggtccgcgc cgttcaggcc
     4681 cgggaacgca cgcgccctgg cgagcgcgtc gtcggcgtcg atcgaatcgc cggcaacgat
     4741 gcaaccatgt tgcgcgcccc tgtcgcggag gatgcgcgtg agcttgcgcg tgtcaatccc
     4801 agagatagcg acgatgccgt tgcgccgcag gtaattcggc agcgactcgg tgctccgcca
     4861 gttcgacgga agccgcggca cgtcgcgcac gatcagtccg gcggcctgca ccttgccaga
     4921 ttcctcgtcg accgcattgc agccggtgat gccgacgtgc ggcaccgtca gcgtgacgat
     4981 ctgctgggcg tacgatgcgt cggtcaggat ctcctggtaa ccggtcatcg aggtgttgaa
     5041 caccacctcg ccgaccgtcg cgcccatcgc tccgattgcg tcgccgtgaa agagggttcc
     5101 atcggcgagg gcgagcatgg cgcggctggg catgggcgga ttccgggtgc aagacaaccc
     5161 tcaggccgcg cggagcgggc ctgtgggtga aagtgttcgg gcgaggcaca atgctagcag
     5221 aaacgcgccc gccattcagg gcgaaccgcc gcgccggcga tacaatgcgc gcaagcttac
     5281 ggacatctgc atgccttccc ccgccctgct cgaccccgcc cgcctggacc gccccgacac
     5341 actcgtccag cgccatccgt tttcgttcct gatcgcgcac ggacaactcc ccgacgcttc
     5401 gcgcgccgat ctcggccgcg acttcccgaa atactccggc gccggattct ttccccatga
     5461 cgccagggaa tgcggcgact ccatcaacga gctcgtcgag gccatcaccg cgccggaatt
     5521 cgcgagcgcg gtcggcgcca agctgggtat cgacgacttg gggcaatatc ccacgctggt
     5581 gaccatctgc cggcatttga acaagcgcca cggcaccatt cacaccgaca gcaagtccaa
     5641 ggtcgcgacg gccctgctct acctgaactc gagctggaac gacaccagcg atggctgcct
     5701 gcggttcctc cagagcgtcg acgacatcga tgcgacgatc gcgccggaag tgaaaccgct
     5761 gtacggcgag ttcgtgatct tcaagcgcgc cgacaattcc ttccacggcc acttgccatt
     5821 cgagggtgaa cgccgcgtca tccaggtcgc ctggctgacc agcgaggaag agaagctgcg
     5881 caagacccag cgcggaaccg cgacgcggtt cttcaagaag ctcgccggaa gattggaccg
     5941 caagttcggc gcgggccgcg accgcagcgc cgcgcatcgg aattgaacct cgggactcgg
     6001 gactcgggac tcgggacagc ataaccaccc tcgtcgtcat tccggcgccg tccgcggctc
     6061 catgaaagga ttcctagccg cgcgagccga tcacatcccc gatcgaatac atccctggcg
     6121 gcttggtcgc aagccacgcc gcggcggcga gggcgccgcg tgcgaagatg ccgcggtcgg
     6181 tggcgcgatg ggaaagttcg atgcgctcgc cggccgtggc gaggatggcg gtgtgttcgc
     6241 ccacgatgtc gcccgcgcgg atcgcggcga aaccgatcga ccctgccggt cgcgagcccc
     6301 cctgtccttc gcgcgacaac accgcaaccg attcgaagtc ctgtccgcgc gctgacgccg
     6361 cggcgagacc caacgccagt gcggtgccgg agggcgcatc ggtcttgcgc acatggtgcg
     6421 cttcgaggat ttcgaggtcc cagtccggca gggccgcggc ggcatcgcgc aatgcgcgcg
     6481 tgagtaccgc gataccgagg ctgaaattgg cggagtggag agccgggatc tcgcgggctg
     6541 cctcatcgag cgcggcttgc cgggtcgcgt ccagcccggt cgtgccgctg acgaacacga
     6601 ttcgatgctc ccggcaatgc gccagcgccg catcgaagcc ggccggcgtg ctgaagtcga
     6661 tcatggcatc cagcttcggc gcattcgtcc atccggacga tgcattcacg cgatgcgcca
     6721 actcgaagcg cccgtcctcg cgcaccagcg cctgcagcac ccgacccatg cggccgtcgg
     6781 cgccattgat ggcaagtctg atcggggagg acatggctag agccgtgcag gaatcgaggt
     6841 gaacaggcac cagaagcgaa gcataacaac cttcgacatt ccggggccgt tcgcggtttc
     6901 atcgcgaacg gaacccggaa tccattttga tcttcctttt tcaagcagag caggatgcat
     6961 tcggggtcgc tgcttcgcag cgtccggagt ggcgaccagg ttgaggcatc ctccccgggt
     7021 gccgactggc ggttctcagc ccgtcatcct cgaccagaac tgcttgacgt tatcgacgaa
     7081 gccactggag cgcggcgagt gcttcttcgc ttcctccgcg tccgcgaagg tggcttcgaa
     7141 ctcctcgagc aactcgcgct gccgcttgtc gagccgcacc ggtgtttcaa ccacgacggt
     7201 gcagtagagg tcgcccgtat ggcggctgcg gacggatttc acgccctgtc cccgcagcct
     7261 gaattcctgg ccggtctggg tctccggcgg cacggtgatg gtggtcgcgc cgccgaggac
     7321 cggcacctcg atggtcgcgc cgagtgctgc ctgcgcgaag cggaccggca cttcgcagcg
     7381 caagttgtcg ccctcgcgcc ggaaaaccgg atgctcgcgc acgtggattt caacgtacaa
     7441 atcgccgggc gtggtgccgg cggcccccgc ctcgccttgc ccggtcaagc ggatgcgatc
     7501 gccgtcatcc acccccgcag gcaccttgac ttccaggctc cgctcgtctt cgaggcggcc
     7561 ttcgccgtga cagtgcttgc acggcgtgac gaccatgcgg ccggcgccgc cgcagtgcgg
     7621 gcaggtctgc tggatggaaa agatcccgtt ctgcatgcgc acgcggccat gcccgcgaca
     7681 agtggcgcaa gtcgacagct tgccgtcctc ggagccggtg ccgttgcaat gatggcagtt
     7741 gaccatcgtc ggcacgcgga tctgtttttc gatgccggcg acggcttcct cgagatcgag
     7801 ttcgagcacg tagcgcaggt cggagccgcg cgcgccgcgc cgaccaccgc cgaagatgtc
     7861 ggagaaaatg tcgccgaaga tgtcaccgac gtcgccgaac ggcgcaccgc cgcgccggcg
     7921 ccccgcgccg ttctcgaatg cggcatggcc gtactggtcg tacagcgcgc ggcgctccgg
     7981 atcgcccagt atttcccacg cttccttggc ttccttgaaa cgctcctgcg cagtcgggtc
     8041 atcggggcaa cggtcgggat gattcttcat cgccaaccgg cgaaaggcct ttttcagttc
     8101 ctcgtcgttc gcggtccgct cgacaccgag gatttcgtag tagcaacgtt tcatgcgcgc
     8161 agtctagctt cgagtcggcg atcgggcacg aaccacaggg ccgcgaccac gccgtagatc
     8221 acgcacgaca gtatgggttc gaaaaacgcc aacgcaatgc cggtgacgta gccgatcggc
     8281 gaaacgatga ccttccagtc atgccggagt gccgacgcaa gcgcgccttc cctgccgccg
     8341 ttggcggcga tcaggctgcg caagagcggt acccacgaca aggctgcgag cagcagcacg
     8401 acgccgtaga gcgcggtcgg caccgccgcg gggacggggt gcgattcgtt caaccaactc
     8461 gtggtgaatg ggaacagcga cagccagaac aggaagaaca ggttcgccca gagcaccttg
     8521 ccgtcgacgt gcgtaaccat ctgcagcatg tggtggtggt tgttccagta gatgccgacg
     8581 tacgcgaagc tcagcacgta gctcaggaat accggaaggc cggccagcaa ggcctgccag
     8641 gtcgcctcgc gcggcagttt cagttcgagc accatgacgg tgatgatcac cgcgatcacg
     8701 ccatcggtga atgcctccat ccggcccttg tgcatgtccg aggttccccg cctctacatg
     8761 gaagttgtcc gcgttgagcg tcgcgcttcc cagcgtcgct cgaacgcgga cagttgatcc
     8821 ggcagcgctc cgcgctgccg gataacactc acttgtcctt gtccttgacc tcggtgaact
     8881 ccgcatccac cacgtcttcc ggcgacgcgc tgccaccacc cggcgcctgc tgtgcgcccg
     8941 gctgcgggcc gggttgcgca ccacccgctt gcgcggccgc gaacagggac tgcgcggctt
     9001 cctcgagctt cgagatccgc gattcgatcg cgtccttgtc gtcgcccttc atcaccttct
     9061 cgagctcgga cagcgattcc tccacccgcc cgatcacgtc gccgcccacc ttgccgccgt
     9121 gctccttgat ggctgagcgg gtggcgtgca ccagctgatc ggccttgttg cgggtgacca
     9181 ccagctcctg gaacttcttg tcttcctcgc ggtgcgtttc cgcatcggag accatctgct
     9241 ggatttcctc ctcggaaaga cccgagccgg ccttgatctc gatgcgctgt tccttgccgg
     9301 tgttcttgtc cttggccgtg acgtgcagga tgccgttggc atcgatgtcg aacgacactt
     9361 cgatctgcgg catgccgcgt ggcgcggcct ggatgccggc gagatcgaac ttggccagcg
     9421 acttgttgaa gcgggcctgg tcgcgttcgc cctgcagcac gtgcacggtg acggcgctct
     9481 ggttgtcctc ggcggtcgag aacacctgcg aagccttggt ggggatcgtg gtgttcttgt
     9541 cgatcagctt ggtcatcacg ccacccagcg tctcgatgcc cagcgacaac ggcgtcacgt
     9601 cgagcaacag cacgtccttg acgtcgccgg aaagcacgcc accctggatc gccgcgccca
     9661 ccgcgacggc ttcgtcggga ttcacgtcct tgcgcggctc cttgccgaag aagtccttga
     9721 ccgcttcctg caccttgggc atgcgggtct ggccgccgac caggatcacc tccgaaatgt
     9781 cggacacccg caggttggcg tcgttgagcg cagtccggca cggctcgatg gtgcgcttga
     9841 ccaggtcctc gaccagcgct tccagcttgg cgcgggtcag cttgatgttg aggtgcttcg
     9901 gaccggatgc gtcggccgtc acgtacggca ggttcacttc ggtctggtgc gccgacgaca
     9961 gctcgatctt ggcgcgttcg gcggcgtcct tcaggcgctg cagcgcgagc ggatcgttgc
    10021 gcaggtccac gccctggtcc ttcttgaatt cgtcgaccag gtaatcgatt acgcgcttgt
    10081 cgaagtcctc gccgccgagg aaggtgtcgc cgttggtggc cagcacctcg aactgcttct
    10141 cgccatcgac ctctgcgatt tcgatcagcg acacgtcgaa ggtgccgccg cccaggtcgt
    10201 acaccgcgat cttgcggtcg ccgccgccct tgtcgaggcc gtaggcgagc gcggccgcgg
    10261 tcggctcgtt gatgatgcgc ttgacttcga ggccggcgat cttgccggcg tccttggtgg
    10321 cctggcgctg cgagtcattg aagtaggcag gcaccgtgat taccgcgtcg gtgatcgtct
    10381 ctccgaggaa atcctcggcg gtcttcttca tcttcatcag caccttggcg gaaatttccg
    10441 gcggagccat ctgcttgccg tcggaggttt ccagccacgc gtcgccattg gagtgctggg
    10501 tgatcttgta ggggacgacg tccaggtcct tctgcacttc ggcgtcggtg aacttgcggc
    10561 cgatcaggcg cttcaccgcg tagaaagtgt tcttgggatt ggtgaccgcc tggcgcttgg
    10621 cgggcgcgcc caccagcacc tcgccgtcct tggaaaacgc cacgatcgat ggcgtggtgc
    10681 gatcgccttc ggaattctcg ataacgcgcg tcttggtgcc ttccatgacc gccacgcacg
    10741 aattggtcgt gccgaggtcg ataccgatga tcttgctcat gtgtcagctc ctggattggg
    10801 tggcggcccg aagccgccta atctcgaaca taaactgggg gttggcggac ctttctcaag
    10861 gcaaaaaccc gggggtcaga gtcgactttc tggccaagcc gctgattcag cggcagggag
    10921 tggatcgaaa gtccactctg accccgcgct tcgattcgcg ccgggcgatc actccttcgc
    10981 aaccgtgacc aatgccggcc gcagcagccg gccgttcagg acgtagcctt tttgcatcac
    11041 gccgaccacg gtgccaggcc gcgcgccgga cgcctcgacc atgctcacgg catgatggcg
    11101 ctccggatcc aacggttcac cgacgggatc gacctgctcc aggccattgc tctcggcgat
    11161 cttcaacagc gacttcaagg tgaggctgag gccttcgcgc atcgacgcca cgtcggcggt
    11221 ctccacggcc agcccgcttt cgaggccgtc gtacaccggc agcaggtcgc gcagcaattt
    11281 ctcgttcgcg aacttcaggc tttgctcgag atcgcgctgc atccgcttgc gctggttctc
    11341 gaggtcggcg cgttcgcgca acagcgtctc gcgcatgccc gcaaggctgg tctccgcttc
    11401 ggccagttgc cggctcagcg tttccagctc gtcgccatcc ggggcaccgg ccggatcggc
    11461 cggccgatcg tcttgcgggt ccccgcctgg cgatccgcgc gattcgttgg ggttcatcat
    11521 gtcgattcct cgtatcgcgg tatggcgatc gtccctggtc gccggtatcg gaggcacccg
    11581 tccatggctg cattttcgtg cacgaagccg cgctatcccg cgccccggca gggaacgcga
    11641 accaaggcgc aacaggcaaa cttatgtggc cgggatgccg cgattcaagg cgtccgtcag
    11701 caggtgggcc gttgcctgca cgaccggaat caccgaatgg taagccatgc gggtggggcc
    11761 gat
//
LOCUS       DASSNH010000149        20591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_402063, whole genome shotgun sequence.
ACCESSION   DASSNH010000149 DASSNH010000000
VERSION     DASSNH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 20591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20591
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402063"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFK00_05580"
     CDS             <1..751
                     /locus_tag="VFK00_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET7592457.1"
                     /translation="DERSAELTKYAANAMLATKISFMNEIANIAEHVGADVEMVRHGI
                     GADPRIGYHFIYPGAGYGGSCFPKDVQALERSAITNGYDARLLGAVEAVNRDQKRKLF
                     DLVSRHFDGNLRGRTIALWGLAFKPNTDDMREAPSRVLMEALWQAGAKVRAFDPQARE
                     ETARIYGAHDDLVLCDDPWTTLQGADALAVATEWKVFRSPDFARVKAMLKTPVLFDGR
                     NLYDPAMVEDAGLAYYGIGRGRSVRRSGSAK"
     gene            748..963
                     /locus_tag="VFK00_05585"
     CDS             748..963
                     /locus_tag="VFK00_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HET7592458.1"
                     /translation="MTRPSDERMDELELKLTFLDDAVTSLNDSEVQQSQRLLKLERAL
                     TELRQELAALRTSLSDDVHNEPPPPHY"
     gene            1190..1711
                     /locus_tag="VFK00_05590"
     CDS             1190..1711
                     /locus_tag="VFK00_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 family protein"
                     /protein_id="HET7592459.1"
                     /translation="MSESLRDQLLKAGFKPAPKPKPLPHPHPRKPEPRKSGHAGEPDL
                     ARAFAIRAKVERAEREAAEREAREKAAAKKERKRKLQALLAGKMLNRDDAEQVRNFEF
                     AGKIRRVYVTAEQLRQLNAGELGVVMHGGRALVCSHEIALQAQAIAPEAVALLVDPHA
                     PPEDDGVPDDLMW"
     gene            complement(1763..3832)
                     /locus_tag="VFK00_05595"
     CDS             complement(1763..3832)
                     /locus_tag="VFK00_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7592460.1"
                     /translation="MFDGLRFSHWKHSASTDGVLVLALDRAGSSVNALSRGVLDELDS
                     MLERIAIVRPAAVVIHAAKPSGFAVGADLKEFEGYARKGTIREEIEHGQRVFERLARL
                     PMPTVAAIHGACMGGGTELALACRMRVAARMPETRIALPEVQLGIHPGWGGTARLPRL
                     IGAPRALAMMLTGRPSSAAHALRDGLVDRLAEPDGLIEAARQLALHPIARPLPQRVRA
                     WLTNCWPVRQVLAPMVRRQAAAKAPPRHYPAPSALVQVWRRGGGDIHQRLRLEARSVA
                     RLAETPTAHNLIRVFFLRERMRSMAGDTVHDIRHVHVVGAGVMGGDIAAWSALRGFEV
                     TLQDREMKFVEPALVRARGMFEKKLKAPDRIAAATNRLKADVEGAGVAAAQLAIEAIY
                     EEPDAKQDVYRTVEPRIADDALIATNTSSIPLDELRGALMQPGRFLGLHFFNPVAQMP
                     LVEVVRHDALDPKIEKRALAWVKAIGKLPLPVQGTPGFLVNRILMPYLLEAMRCHTEG
                     IPDPAIDRAAKKFGMPMGPIELADTVGLDVALSVGRELGPFLGLELPEGIADKVEAGK
                     RGKKDGQGFYVWKDGKPEKPKVDSNYRAPADLEDRLILPMLNEAVACLHDGVVVDADL
                     LDAGVIFGTGFAPFRGGPIQYIRSTGAGALREKLEALAQAHGPRFAPKQGWFNPALAQ
                     PGRAESA"
     gene            4001..6256
                     /locus_tag="VFK00_05600"
     CDS             4001..6256
                     /locus_tag="VFK00_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7592461.1"
                     /translation="MPGDYSVLVMATSRDDRRVLFDALDGFAPGKILSARDATHAQAL
                     LQHGRTPALAILDFEHATDEALLLCTGLSEVPVIGLFGAVLDAHPDDPRLTECNNIRA
                     WLRIPADATEARLRIGEVLGGAGSSNPAMAAGGARPLLANERREPDLMVVLRSLAEAF
                     QSGRDTASLPRLLERAMEALRLDLIAVAGRDASGKALEPLARMDRLSAPGAPDPLAAP
                     FVQRALQGHATVSTADPPADPADAGFVDATSCTRYAALPLLDGRRNVLGVLACASRRA
                     DALPPAMLQPLLEILAARFAAVLELRAERERVRGRALLDGLTGLPNRMLFNDRLESIL
                     QDARRTGEIFAVLFVDLDRFKGINDSLGHRIGDQVLVAEAQRLRALLRASDTVARYAG
                     DEFIVILRHVVDRADIPRAAGMLLEGMQAPLVLDNGSELQVTASIGVSIYPDDAADAE
                     GLIVHADRAMYSAKGQGRNQVQAFGSVPGDGHRQYREMESRLRDAEANGELCVHYQPQ
                     VEIASGDIIGVEALLRWRHPVLGVLSPGFFLPLAEETGLIIPVGEWVLRRACADVAEW
                     RRSAGATLRLAVNLSALQWLRPNLVTMIEGACRDAGIEPRLLDLEATESVLAHPPPEL
                     EATVLALRRIGCRIVVDDAGAGHLAPDEATEVPIDAVKIDHSFVRNIGADPDDEAVIT
                     SMLDAAVRQGRQVIAEGVETERQLEFLRERGCEAAQGYLFHRPLPAASIASLLAERPD
                     PSTQRGRADAA"
     gene            6447..7034
                     /gene="rpoE"
                     /locus_tag="VFK00_05605"
     CDS             6447..7034
                     /gene="rpoE"
                     /locus_tag="VFK00_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HET7592462.1"
                     /translation="MGDVETDRALVARVRKGDSKAFDLLVRKYQHKIIGLVSRYVHDW
                     SECEDVAQEAFIRAYRAIGAFRGDSAFYTWMYKIAVNTAKNHLVAQGRRPPAEDIDAD
                     TAVQFDSGTRLRDEATPEHELARREIEQTVFATVEKLPEELRDAITLREVDGLSYEEI
                     AERMGCPIGTVRSRIFRAREAIDEQLRPLLSNDDT"
     gene            7202..7876
                     /locus_tag="VFK00_05610"
     CDS             7202..7876
                     /locus_tag="VFK00_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HET7592463.1"
                     /translation="MDPTSREDLSALMDGELAAEPTRFLLRRLDHDPELGATWSRWHL
                     IRACLASDSARMSAQRRDNDFAAGVAAALETESAPPSRTRHWARYLGGGAIAASVAVA
                     ALMLNVPASRIDAPAPVAQDRPVSNPVGSALAATGLAAAPAKADAGIPPWLLSRQPTV
                     FGAQPASANVVYGTGLMQPDYLQRATYSRDAAPYASLQAGQPRLPFSIVLLPEQPPAS
                     ASASRH"
     gene            8019..9470
                     /locus_tag="VFK00_05615"
     CDS             8019..9470
                     /locus_tag="VFK00_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET7592464.1"
                     /translation="MLNRARRVAAVFVLLCFSGAGTALAATPTVALPDFTSIVQKYGP
                     AVVNVVAHYKHASEMGDEHPGGQQTQMEPQMPDILRHFFGMPLGPEFRGPTGPAESMG
                     SGFIISSDGYILTNRHVVANADSVKVHLTDHRSFTAKVVGSDKIYDIALLKVDASDLP
                     TVQIGDSDELKPGEWVVAIGSPYGLDHSVSAGIVSYVGRTLGAQDQPAVPFIQTDVPI
                     NRGNSGGPLFNLSGQVVGINSQIFSTTGGAMGLSFSIPINIAMNAAHQLKTNGYVSRG
                     MIGVSIQTVTEGIAKSKGLKQAQGAVVAQVQPDGPAAKAGLQVGDVITGFNGHEIYES
                     SQLPPAVAMTSPGTEATMSIIRDGKPRTLKLKVGEMPRNGLSTELIAGAPAATSGSKL
                     LGLSVQDITPQIRQQLGYSGKGGVVITDVEGPAADAHLSPGDVVLRVGNKPVDNVAAF
                     KRETANVKPGSVVLLLVSRQGQNLFIAVPVPEK"
     gene            9611..10480
                     /locus_tag="VFK00_05620"
     CDS             9611..10480
                     /locus_tag="VFK00_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7592465.1"
                     /translation="MKLYSIAGNTQKLDGGAMFGNAPRAMWSKWVPVDEQNRIPLACR
                     CLLATGLDGRNVLFETGIGAFFEPKLRERYGVVEDRHVLLESLRAAGLSHEDIDVVVL
                     SHLHFDHVGGLLAAWEEGAPPRLLFPNAKYLVSAGHWARALDPHPRDRASFVPGVTEL
                     LEASGRLELVDGEYSETLGKAVRFHWSQGHTPGLMLSEIVGPSDRGGIVYCADLIPGR
                     PWVHLPVTMGYDRAPEMLIDEKRAFLDDKIARGVRLFFTHDHAVAMATPRRDQRGRYG
                     VGDEQAHLAGASL"
     gene            10490..11530
                     /locus_tag="VFK00_05625"
     CDS             10490..11530
                     /locus_tag="VFK00_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HET7592466.1"
                     /translation="MIRLPGLLQDPSRNAALLGAGLLVLGLALLGFTEHADSLRAMGE
                     DALGGFVRTGEAAVPGPGSEGQLVVAVGAPKVEVPARDAQFGIAVDAPALLRKVEMFQ
                     WNETRFGGRPGYELDWFDHPIDSSAFDDADRHANPGAFPINAARFDAPDLTVAGFHLA
                     PELVGMIQGPESFDPDLSHLPANMAATFQPQDGTLVTSEDPGRPRVGDLRIGWLRIAP
                     SELTVFARDENGTLVPARNPQGEPIAQVLIGRHSLTDVLTDAPQPPRFRWARRVLAVL
                     LAWAGITLLLPDRYRHDRLLPLAAAVVPMAILAAIFWFDVRAWAAAGLVAITILAATF
                     AGWRVRRDIREP"
     gene            11533..11925
                     /locus_tag="VFK00_05630"
     CDS             11533..11925
                     /locus_tag="VFK00_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET7592467.1"
                     /translation="MLIAGFYVALGALLVVALAVRVMWLRNTRRVGLGAGGDAVLARA
                     IRAHANAAEYLPIALLLLVLLALEQTRPGLLHMFGIVLIVARILHAIGLSSASGRSFG
                     RMVGIGLTVAVMLAMAILLIVRFALASG"
     gene            complement(11995..12567)
                     /locus_tag="VFK00_05635"
     CDS             complement(11995..12567)
                     /locus_tag="VFK00_05635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7592468.1"
                     /translation="MTHAPRVPADADAPVETLYAGRWLTLRQRGGWEFVERNNPEGAV
                     IIVAVTPEDRVLFVEQYRVPIRQRTIEMPAGLIGDNGHADDDIASTACRELEEETGWT
                     CARVLPLHAGPSSAGMSTEMMHFVRALGLRKIGDGGGDDSEDIVVHEVPRSEAGTWLH
                     AMTQKGYSIDPKLFAGLWFLEHETCSCDRP"
     gene            complement(12564..13502)
                     /locus_tag="VFK00_05640"
     CDS             complement(12564..13502)
                     /locus_tag="VFK00_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET7592469.1"
                     /translation="MDGLKEATPASAAISTLYLIDASMYVFRAWHSMPNVFFDCDGEP
                     VNAVHGFGRFLLDFLERTRATHAVAAFDIALTSSFRNAIYPAYKANREPAPPELKRQF
                     ECCRELAAAFGLTVLADAAYEADDLIGSVLHAAHGQGLRGVIVSADKDFGQLLRDGAE
                     QWDYARGQRWGAAGVSARLGVEAHQVVDYLALTGDAIDNIPGVPGIGPKGAAALLAHF
                     GDLETLLARVDEVPFLRLRGAASMAAKLRVHAVQARLSQRLARIALDAPVPGHPDRLQ
                     RQAPDATALTALFERFRFGPLTRIRALKILQMEAIA"
     gene            complement(13490..14068)
                     /locus_tag="VFK00_05645"
     CDS             complement(13490..14068)
                     /locus_tag="VFK00_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7592470.1"
                     /translation="MRDDHALEFLRNRRSVPAAQLAPPGPTPEQLREIIATAIRVPDH
                     GKLTPWRLIAFTGDAGARYGEALAALHVQVDPGVPGKKLAKDRERFAHSPVVLAVIAR
                     IDERHPKIPAQEQLLSAGCLGFNLLLAAQAAGFAAQWLTGWSCYDPAAAKLLGLAAGE
                     RAIAYIHVGSRESEPPERNRPDVDEVVSAWTD"
     gene            14147..15250
                     /locus_tag="VFK00_05650"
     CDS             14147..15250
                     /locus_tag="VFK00_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7592471.1"
                     /translation="MPITITALRETADGERRVAVTPETAKKFASRGLRVSLEHGAGER
                     AGFPDAAYPGVEFATGDAVAQQADVVLCVQPPAQDRVAGMKQGAALIGQLHPHAPADR
                     LAAYAQRGLAAFSLELLPRTTRAQAMDVLSSQAAVAGYRAMLIAAESAPKLFPMMTTA
                     AGTLRPSKVLVIGAGVAGLQAIATARRLGAMVEGFDVRPETREQIESLGAKFLDLGVS
                     AAGQGGYARELTAEERAAQQARLAEHVKTVDVIVTTAAVPGRPSPRIIPLSMVDGMKA
                     GAVIVDLAAEGGGNCEATTPGERVERDGVTIIGPLNLPSGAPLHASEMYARNLAHFSE
                     LVIADGAWKPDFEDELVAKTCVARDGEVLFGKA"
     gene            complement(15275..15817)
                     /locus_tag="VFK00_05655"
     CDS             complement(15275..15817)
                     /locus_tag="VFK00_05655"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HET7592472.1"
                     /translation="MRPWYQPLRLLALACLIAPPTTVLAQASPAQPAPAAADASGQPL
                     AWSGLSADQQRMLAPMQDQWNQLPASRQQRLAGLAQRWASLPPDRQQQIRDRLSSLAG
                     MTPAQHHQLRENARAFRNLTPEEREKVSAAFRKFQSLPPDQRRALRERWQKMTPEQRG
                     RWNPSGGRRARPSPRSSGGS"
     gene            complement(15820..16263)
                     /locus_tag="VFK00_05660"
     CDS             complement(15820..16263)
                     /locus_tag="VFK00_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592473.1"
                     /translation="MTDLNHNNLNSLHSDDEVIARARSLFKSACENTDSYHALRLGLA
                     RRKALNAGAGHRFGGFWTALAGTAVACCVLTLGAVWMRAGGQGGQAAAIASTHAAITA
                     ESAGHQEAGVPDVGNGQMEMVEDLAFYRWLATQPALSSAGSRGSR"
     gene            complement(16260..16850)
                     /locus_tag="VFK00_05665"
     CDS             complement(16260..16850)
                     /locus_tag="VFK00_05665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7592474.1"
                     /translation="MTAAAALEAVPSQALDIERSSPASLEAFLASVERRAWRMAEIAL
                     RDPDEALDAVQDAMLRLVRHYAGKPAEEWAPLFWGILRRRITDLQRRRTVRNRVLAWT
                     GHAASDDAETLPAWEAPDQGPDPPRMLASRQAHAAMSKAIRALPRRQQQAFLLRVLEG
                     LDVADTSKAMGCSEGSVKAHLSRAMDALRTQLEDWR"
     gene            17000..17311
                     /locus_tag="VFK00_05670"
     CDS             17000..17311
                     /locus_tag="VFK00_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7592475.1"
                     /translation="MITGFLALYIFMLAAFTGYEIISRVPVILHTPLMSGSNFVHGIV
                     LVGAMVALGHAETPAEITIGVIAVLLSAGNVAGGYVVTERMLEMFKASKPASPRSVEK
                     K"
     gene            17311..18780
                     /locus_tag="VFK00_05675"
     CDS             17311..18780
                     /locus_tag="VFK00_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7592476.1"
                     /translation="MALPGWVPTVVEAIYFIAAVLFIWGLKRMSSPRTARRGIIWAGI
                     GMAAAILVTFFLPGMHNLGWMIGAIVLGVGLSWWVGRRVPMTAMPQMVALFNGMGGGA
                     AAAIGAVELIRFNAALTLIPDTLPVQPEAWATISGHLGAVEMTLAFVGALIGAISFTG
                     SLIAFAKLQGWMDRRFVFPLQRWVNLAIFVAAVVCGALALAAGIEGHAFAWGLLAAFF
                     VLSLVFGVAMTLPIGGADMPVVISMYNAFTGLAVAFEGFVLQNEAMIIAGMVVGAAGT
                     LLTQLMAKAMNRSLGNVLFGNFGAGSGAAQQEIAGALKPIDAADAAVMMAYAERVAIV
                     PGYGMAVAQAQHKIWEFAKLLIDRGVKVKFAIHPVAGRMPGHMNVLLAEAGVPYDLIE
                     DMDDINPEFPNIDVALVIGANDVVNPVARTDKNSPIYGMPILDVANAKNVIVIKRGRG
                     KGFAGIENALFYADNTRMLYGDGQAAAGELVAGLKNVDA"
     gene            complement(18917..19408)
                     /locus_tag="VFK00_05680"
     CDS             complement(18917..19408)
                     /locus_tag="VFK00_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592477.1"
                     /translation="MDDPAHSMSAAKSARKPAAILAFVAGGLTSAFGWLMCTLATGAL
                     AAFAALVLGIRPSWLILVLALPLALVLKLCGCLVERWAGPIAALAVLLAGFYAVSLVA
                     IARIAAVTGFPFGLAFRTGGFGLTLQVAELGLDAIAVLVYAAAAVAAALLATWLTRPP
                     ARR"
     gene            19610..20167
                     /gene="sufT"
                     /locus_tag="VFK00_05685"
     CDS             19610..20167
                     /gene="sufT"
                     /locus_tag="VFK00_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HET7592478.1"
                     /translation="MPGFSASSEPFTLQRDCHAVHVPYGDVVNLPAGQTGYITQSLGG
                     SFTVYVDGSLFRIDGKDADALGKAPPEPLRVPENAGDEDVEVIVWEQLRTCFDPEIPI
                     NIVDLGLVYDLSMEHLEDGQRKVYVKLTLTAPGCGMGDILVEDARTKLEMIPTVAEAQ
                     IDLVFDPPWNQAMMSDEAKLQTGMM"
     gene            complement(20273..>20591)
                     /locus_tag="VFK00_05690"
     CDS             complement(20273..>20591)
                     /locus_tag="VFK00_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592479.1"
                     /translation="MATTKAFNLGLLGIIELLLDGRSTDAQRDTHPCDAIRIRDAYSP
                     PGGETAKSLLFPRMQHAGRHGARADCPPGALNTGWDLPNVNKCKYPVTLRCNNPFSAR
                     TAT"
BASE COUNT         3307 a         6798 c         7092 g         3394 t
ORIGIN      
        1 ggacgagcgt tcggccgaac tgaccaagta cgccgccaac gcgatgctcg cgaccaagat
       61 cagcttcatg aacgagatcg ccaacatcgc cgaacacgtc ggcgcggacg tcgagatggt
      121 gcgccacggc atcggcgcgg acccgcgcat cggttaccac ttcatctacc ccggcgccgg
      181 ctacggcggt tcatgtttcc cgaaggacgt ccaggctttg gaacgcagcg caatcacgaa
      241 tggctacgac gcgcggctgc tgggggcggt ggaagcggtc aaccgcgacc agaagcgcaa
      301 gttgttcgac cttgtctccc gtcacttcga cggcaatttg cgcggcagga ccatcgcgct
      361 gtgggggctc gccttcaaac ccaacaccga cgacatgcgt gaagcgccga gtcgcgtcct
      421 gatggaagcg ctttggcagg ctggcgcgaa ggtgcgtgcg ttcgatccgc aggctcgcga
      481 ggaaaccgcg cgcatctacg gcgcgcacga tgaccttgtg ctatgcgacg atccgtggac
      541 taccctgcag ggcgccgatg cgctggcggt ggcgactgaa tggaaggtgt tccgcagccc
      601 tgatttcgcg cgcgtcaagg cgatgctgaa gacgccggtg ctgttcgacg gccgcaacct
      661 gtacgacccc gcgatggtcg aagatgcggg gctcgcgtac tacggcatcg ggcgcgggcg
      721 cagcgtgcgt agatccggga gcgcaaaatg accaggccat ccgacgaacg catggacgaa
      781 ctcgagttga agcttacgtt cctggacgac gcagtgacat cgttgaacga cagcgaggtg
      841 cagcagtcgc agcgcctgct gaagctcgag cgcgcgttga ccgaactgcg ccaggaactc
      901 gcggcgttgc ggaccagtct gtcggatgac gtgcacaacg agcccccacc gccgcattat
      961 taatgcgatc atccatgatc gccgcatcgc ccatgttcgt tgctcttgaa cggccctagc
     1021 atttgcgaca ccagaacggc catccatggc ctgacttttc acaaaaaccg cttcgagcgc
     1081 ggccatcgct tgcaacagcg tccagtcccg atgccatccg atccacctgt cgtcattccg
     1141 gggccgtccg cgctgtttgc ggaccgaatc cggaatccat tttcgtgcca tgagtgaatc
     1201 cctccgcgac cagctgttga aggccggctt caagccggcg ccgaagccaa agccgctgcc
     1261 gcacccgcac ccgcgcaagc cggaaccccg caagtcgggc catgcgggag agcccgatct
     1321 tgcccgggcc ttcgcgatcc gggcgaaagt cgagcgggcc gagcgcgaag ccgccgagcg
     1381 cgaggcgcgc gaaaaggccg cggcgaaaaa ggaacgcaag cgcaagctgc aggcattgct
     1441 cgccggcaag atgctcaatc gcgacgatgc cgagcaggtc cggaacttcg agttcgccgg
     1501 caagatccgt cgcgtctacg tcacggccga acagcttcgc caactcaacg ctggcgaact
     1561 cggtgtggtg atgcacggcg ggcgggcgct agtctgctcc cacgaaatcg cgctgcaggc
     1621 gcaggcgatc gcccccgagg ccgtcgcgct cctggtcgat ccgcatgcgc cgccggaaga
     1681 cgacggcgtt cccgacgacc tgatgtggtg acgacgcatc tgccgtcgcg tacatgtcgt
     1741 gcaagggagc ccgcggaagc gtctacgccg attccgccct gcctggttgc gccagcgccg
     1801 gattgaacca gccctgtttt ggtgcgaatc gcgggccgtg cgcctgcgcg agtgcctcca
     1861 gtttttcgcg cagcgcgccc gcgccggtcg agcggatgta ctggatcgga ccgccgcgga
     1921 agggcgcgaa gccggtgccg aagatcacgc cggcatcgag cagatcggca tccacaacga
     1981 ccccgtcgtg gaggcacgca acggcttcgt tgagcatcgg caggatcagg cggtcttcga
     2041 gatcggccgg cgcgcggtag ttcgaatcga ccttcggctt ttcgggtttg ccgtccttcc
     2101 atacatagaa gccctggccg tccttcttgc cgcgtttgcc cgcctcgacc ttgtcggcaa
     2161 tgccttccgg gagttccagg ccaaggaacg gccccagttc gcgccccacc gacagggcga
     2221 catcgagccc aaccgtgtcg gcgagttcga taggccccat gggcatgccg aactttttcg
     2281 ccgcgcgatc gatcgcagga tccgggatgc cttcggtatg gcagcgcatg gcttccagca
     2341 ggtacggcat caggatccgg ttgaccagga acccgggcgt tccctggacc ggcagcggca
     2401 gtttgccgat cgccttcacc cacgcgagcg cgcgtttctc gattttgggg tcgagcgcgt
     2461 cgtggcgcac gacttcgacc agcggcatct gcgcgacggg attgaagaag tgcagtccga
     2521 ggaaccggcc gggttgcatc aatgcgccgc gcaattcatc cagcggaatg gaagacgtgt
     2581 tggttgcgat aagcgcgtcg tccgcgatcc tcggctcgac cgtgcggtag acgtcctgtt
     2641 tggcgtcggg ttcctcgtag atcgcctcga ttgcgagttg cgcggcggcc acgccagcgc
     2701 cttccacgtc ggccttgagg cgattggtgg cggcagcgat gcgatccggc gccttcagct
     2761 tcttttcgaa catgccgcgc gcgcggacca gcgcgggctc gacgaatttc atctcgcgat
     2821 cctgcagcgt gacctcgaag ccgcgcagcg cgctccaggc cgcgatgtcc ccgcccatca
     2881 cgccggcgcc gacgacgtgc acgtggcgga tgtcgtgcac cgtgtcgcct gccatcgaac
     2941 gcatgcgttc gcgcaggaag aacacgcgga tcaggttgtg cgccgtcggg gtttcggcga
     3001 gcctggcgac cgagcgtgcc tccagcctca gccgctgatg gatgtcgccg ccgccacggc
     3061 gccacacctg gaccagtgcg gagggcgcgg gataatgccg gggcggggcc ttcgccgcgg
     3121 cctgccgccg caccatcggc gcgagtacct gccgcaccgg ccagcagttg gtgagccagg
     3181 cgcgcacgcg ctgcggcagg ggccgcgcga tgggatgcag cgccaactgc ctcgccgctt
     3241 cgatcagccc atcgggttcc gcgagtcggt cgacgaggcc gtcgcggagg gcatgggcgg
     3301 cgctggaagg cctgccagtc agcatcatcg ccagcgcccg gggcgcaccg atcaggcgtg
     3361 gcaggcgcgc ggttccgccc cagccgggat ggattccgag ttgcacctcg ggcaaggcga
     3421 tccgggtttc cggcatgcgc gcggcgaccc gcatgcggca cgccagcgcg agttcggtgc
     3481 cgccgcccat gcacgcgccg tggattgcgg cgacggtggg catcggcagc cgggccagcc
     3541 gctcgaacac ccgctggccg tgttcgattt cctcgcgaat cgtgcccttg cgcgcatatc
     3601 cctcgaattc cttgaggtct gcgccgaccg cgaagcccga gggcttggct gcatggatca
     3661 cgaccgccgc cggccgaacg atcgcaatcc gttccagcat cgagtccaat tcatcgagga
     3721 cccctcgtga cagtgcgttg acgctgctgc cggcacgatc cagcgccagg acgagcacgc
     3781 cgtccgtgct ggctgaatgc ttccagtggc tgaatctcaa accgtcgaac acttctcgtt
     3841 cccgaggtcg gctcctgagc atcatcgacc gggcagggat tggggtcaaa cgtccgaacg
     3901 aggcgattac cgtggcacag gatggctcga aaacggaatt ggcggacgat cggttgctgc
     3961 attaccctca cgcgcacata cagcaagtgg cgttgcggtg atgcccggcg actactcggt
     4021 gctggtgatg gcgacatcgc gcgatgatcg gcgcgtgttg ttcgacgccc tggatggctt
     4081 cgctccgggc aagatcctga gcgcgcgcga cgccacccat gcgcaggcgt tgttgcagca
     4141 tgggcgaacg ccagcgctgg cgatcctcga cttcgaacat gcgaccgacg aggcgctgtt
     4201 actttgcacc gggctcagcg aggtaccggt gatcggcctg ttcggcgcgg tgctggatgc
     4261 gcatcccgac gatccacgcc tgaccgaatg caacaacatc agggcatggt tgcggatccc
     4321 cgccgacgct acggaagcgc gcttgcgtat cggcgaagtg ctcggcggcg cgggatcgtc
     4381 gaaccccgcg atggcggcgg gcggcgcacg tccattgctc gccaacgagc ggagggagcc
     4441 cgatctcatg gtcgtgctgc gttcgctggc ggaagcgttc cagtccggcc gggatactgc
     4501 gagtttgccc cgcctgctcg aacgcgcgat ggaagcactt cggttggacc tgatagcggt
     4561 ggccgggcgc gatgcgtccg gcaaggccct cgagccgctc gcgcggatgg atcgcctgtc
     4621 cgcgccggga gcgcccgacc cgcttgcggc tccgttcgtg caacgggcgt tgcagggcca
     4681 cgccaccgtt tccacggctg acccgcccgc cgatccggcc gacgccggtt tcgtggatgc
     4741 cacaagctgc acccgctacg ctgcgttgcc gctgctggat ggacgtcgca acgtgctggg
     4801 cgtgctcgcg tgcgcatcgc ggcgcgcgga cgcgctgccg cctgcgatgc tgcagccgct
     4861 gctcgaaatc ctcgccgcga gattcgccgc ggtgctggaa ttgcgcgcgg agcgcgagcg
     4921 cgtacgcggc cgtgcgctgc tggacgggct taccggcttg ccgaaccgga tgctgttcaa
     4981 cgaccggctg gaatccatcc tgcaggatgc gcgccgcacc ggcgaaatct tcgcggtgct
     5041 gttcgtggac ctcgaccgct tcaagggcat caacgattcg ctgggccatc ggatcggtga
     5101 ccaggtgctg gtcgcggagg cgcagcggct gcgtgcgttg ctgcgggcct ccgacaccgt
     5161 ggcccgctat gcgggcgacg agttcatcgt gatacttcgc catgtcgtcg atcgcgccga
     5221 catcccgcgc gccgcgggca tgttgctgga gggcatgcag gcgccgctgg tgcttgacaa
     5281 tggttccgaa ttacaggtga cggcgtcgat cggcgtgagc atctatccgg acgacgcggc
     5341 ggatgccgag ggcctgatcg tgcacgccga cagggccatg tacagcgcca aggggcaggg
     5401 gcgcaaccag gtgcaggcgt tcggcagcgt cccgggcgac gggcatcgcc agtaccgcga
     5461 aatggagtcg cgactgcgcg atgccgaagc caacggcgag ttgtgtgtcc actaccagcc
     5521 ccaggtcgag atcgcatccg gggacatcat cggcgtggag gccctgttgc gctggcggca
     5581 tccggtgctg ggcgtgctga gccccgggtt cttcctgccg ctggcggagg agaccgggct
     5641 gatcatcccg gtcggcgaat gggtgctgag gcgcgcctgt gccgacgtgg cggaatggcg
     5701 ccggagcgcg ggcgcaacgc tgcgcctggc ggtgaacctg tcggcgctgc agtggctgcg
     5761 ccccaacctg gtgacgatga tcgagggcgc ctgccgcgac gccggcatcg agccgcgcct
     5821 gctcgacctg gaggcgaccg agagcgtgct cgcacatccg ccgccggagc tggaggccac
     5881 cgtgctggca ctccgccgga tcggctgccg catcgtggtg gacgacgccg gcgcggggca
     5941 cctcgcgccc gacgaggcga cggaggtgcc gatcgacgca gtcaagatcg accacagctt
     6001 cgtgcgcaac atcggcgccg atccggacga cgaagcggtg atcacttcaa tgctggacgc
     6061 ggccgttcgc caggggcggc aagtgatcgc cgagggcgtc gaaaccgagc gccagctcga
     6121 gttcctgcgg gagcggggct gcgaagccgc gcagggctat ctgttccacc ggcccctgcc
     6181 ggccgcctcc attgcgagcc ttctcgccga gcggccggat ccatcgacgc aacgcggtcg
     6241 cgcggacgct gcctagcggc gcccgcagcg cttgattcac ccgatctgcg ttaggcttga
     6301 tccgggtcgc cggcgaccca accatgttca agagagcaga cggggcgagg gataggcaaa
     6361 tggcggaacc ggttgaactt tcgcgcgccg ggcgcggtca caccggggcg cacgctgcgt
     6421 ccggtgccgt agggagtgcc gcccggatgg gcgatgtgga aaccgatcgc gccctggtcg
     6481 cacgcgtgcg caaaggtgac agcaaggcat tcgatttgct ggtgcggaag taccagcaca
     6541 agatcatcgg cctggtgtcg aggtacgtgc acgactggag cgaatgcgag gatgtcgcgc
     6601 aggaagcctt catccgcgcc tatcgcgcca ttggtgcatt ccggggcgac agcgcgttct
     6661 acacctggat gtacaagatt gcagtgaaca ccgccaagaa ccacttggtc gcccagggcc
     6721 ggcggccgcc ggccgaggac atcgacgcgg acaccgcggt gcaattcgat tccggcaccc
     6781 gcctgcgcga cgaggctacc ccggaacacg aactggcgcg ccgggagatt gaacaaaccg
     6841 tgttcgccac ggtcgaaaag ctgccggagg aattgcgcga cgcgatcacc ttgcgcgagg
     6901 tcgatggact gtcctacgag gagatcgccg aacgcatggg ttgcccgatc ggcaccgtgc
     6961 ggtcccggat cttccgcgcc cgcgaagcca tcgacgaaca attgcggcca ctgctttcca
     7021 atgacgacac ctgaagcacc acccgccgag ccaggcatcg ccgcgccggc ggaccgcgga
     7081 ccccggaaca cgtctcgccg aggtaaacgc gggaccgagg ctgcccggga tgaaacccgg
     7141 accgctttct cgatgtcata tggtcggtag ccgacccgaa gtgcgagtga ggaacccgga
     7201 gatggacccg accagccgcg aagacctgtc cgccctgatg gatggcgaac ttgccgccga
     7261 accgacccgc ttcttgctgc ggcgcctcga ccacgatccc gaactcggcg cgacctggtc
     7321 gcgctggcac ctgatccgcg cgtgcctggc ttccgattcc gcgcggatgt cggcgcagcg
     7381 ccgcgacaac gatttcgccg cgggcgtcgc ggctgcactc gaaaccgagt cggccccgcc
     7441 gtcgcggacc cgccactggg cacgctatct cggcggcggc gccatcgccg cgagcgtcgc
     7501 ggtcgcggcc ctgatgctca atgtgcccgc ttcgcgtatc gatgcgccgg cgcccgtcgc
     7561 gcaggaccgg ccggtgtcga atccggtggg ttcggcgctg gccgccaccg gactggctgc
     7621 tgccccggcc aaggccgacg ccggcattcc gccgtggctg ctttcgcgcc agccgaccgt
     7681 cttcggggcg caaccggctt cggccaatgt cgtttacggc accggcttga tgcaacccga
     7741 ttacctgcag cgcgcgacct attcgcgtga tgccgcgccc tacgcatcgt tgcaggcggg
     7801 ccagccgcgg ctgccgttct cgatcgtatt gttgccggag cagccgcccg cgagcgcgtc
     7861 cgcctcgcgg cactgaggtg ccgtggttcg cggcgagccg cgaaccaccc tgtggatccg
     7921 cgcacgcgcg caattcctgc tggctccggc cgcgcgcggg tgatccgtat cgcgccgaca
     7981 gcggcggcaa ggcttttgca aatcgaaagg agaaccgtat gttgaaccgg gcgaggcggg
     8041 tcgccgccgt attcgttctg ttgtgctttt ccggcgccgg cactgcgctg gccgccacgc
     8101 cgaccgtggc gctgccggac tttaccagca tcgtccagaa atacgggccg gccgtggtga
     8161 acgtggtggc ccactacaag cacgccagcg aaatgggcga cgagcatccg ggcggccagc
     8221 aaacccagat ggaaccgcag atgccggaca tcctccggca tttcttcgga atgccgctgg
     8281 gaccggagtt ccgtggccct accggcccgg ccgaatccat ggggtcgggc ttcatcatct
     8341 cgtccgacgg ttacatcctc accaaccgcc acgtggtcgc caacgccgat tcggttaagg
     8401 tgcacctgac cgaccatcgg agcttcaccg ccaaggtggt cggctccgac aagatctacg
     8461 acatcgcgct gctgaaggtc gacgcgagcg acctgccgac cgtgcagata ggtgattccg
     8521 atgaactgaa gcccggcgag tgggtggtcg cgatcgggtc tccctacggc ctggaccatt
     8581 cggtgagtgc gggcatcgtc agctacgtcg ggcgcacgct gggcgcgcag gaccagccgg
     8641 cggtgccgtt catccagacc gacgtgccga tcaaccgcgg caattccggc ggaccgctgt
     8701 tcaatctttc cggccaggtc gtcggcatca attcgcagat cttttccacc accggcggcg
     8761 cgatgggatt gtccttctcg atcccgatca acatcgcgat gaatgccgcg caccagttga
     8821 agaccaacgg atatgtcagc cgcgggatga tcggcgtcag catccagacc gtcaccgaag
     8881 gcatcgccaa gtccaagggc ctcaagcaag cccagggcgc ggtggtcgcg caggtccagc
     8941 cggacggccc cgctgccaag gcgggcctcc aggtcggtga cgtgatcacc gggttcaacg
     9001 gccacgagat ctacgaatcg tcgcaattgc cgccggcggt cgcgatgacg tcgccgggca
     9061 ccgaagcgac gatgagcatc atccgcgatg gcaagccgag aacgctcaag ctcaaggtcg
     9121 gcgagatgcc gcgcaacggc ctttcgaccg agttgatcgc cggtgcgccg gccgccacca
     9181 gcggcagcaa actgctcgga ttgagcgtgc aggacatcac gccccagatc cgccagcaac
     9241 tcggctacag cggcaagggc ggggtggtga tcacggacgt cgagggaccg gccgccgatg
     9301 cgcatctttc gccgggcgac gtggtcctgc gggtgggcaa caagcccgtc gacaacgtcg
     9361 ccgccttcaa gcgcgaaacc gcgaacgtca aaccgggcag cgtggtgctg ctgctggttt
     9421 cccgacaggg ccagaacctg ttcatcgcgg tgcccgtgcc ggagaagtag gtttcgggac
     9481 tcgggactcg gaaggcggaa atgccacccc cttcgcttgc gaagggggtc actgcgtctg
     9541 ccacaggcag gcgcagtggg gggatgctcg agtaccatgt cgtgtcgaca agatcggtac
     9601 gaaatatcgc atgaagctgt actccatcgc aggcaacacc cagaagctcg acggcggcgc
     9661 gatgttcgga aacgcgccgc gcgcgatgtg gtcgaagtgg gtcccggtcg acgagcagaa
     9721 ccgaatcccc ctggcctgcc gctgcctgct ggccaccggt ctcgatggcc gcaacgtgct
     9781 gttcgagacc ggcatcggcg cgttcttcga accgaagctc cgcgagcgct acggtgtggt
     9841 cgaggaccgc cacgtgctgc tggaatcctt gcgggcggcc gggctgtcgc acgaagacat
     9901 cgacgtggtg gtgctgtcgc acttgcattt cgatcacgtc ggcggattgc tcgcggcgtg
     9961 ggaagaaggt gcgccgccgc gcttgctgtt cccgaacgcg aagtacctcg tgagcgccgg
    10021 ccattgggcg cgtgcgctcg atccgcaccc gcgcgatcgc gcctcgttcg tgccgggcgt
    10081 gacggagctg ctggaggcca gcggccggct ggaactggtc gacggcgaat actcggagac
    10141 gttgggcaag gcggtgcgct tccactggtc gcaggggcac acgccgggtt tgatgctttc
    10201 ggaaatcgtc ggcccgagcg accgcggcgg catcgtgtat tgcgccgacc tgatcccggg
    10261 acggccgtgg gtgcacctgc cggtgacgat gggttatgac cgcgcgcccg aaatgctgat
    10321 cgacgaaaag cgcgcgttcc tggacgacaa gatcgcgcgc ggcgtgcggt tgttcttcac
    10381 ccacgaccac gcggtggcga tggccacccc gcgccgggac caacggggcc gctacggggt
    10441 cggcgacgag caggcgcacc tagcgggggc ttcgttgtaa gctcgcggcg tgatccgcct
    10501 gcccggactc ttgcaggacc cgtcgcgcaa cgcagcgttg ctgggcgcgg gcctgctggt
    10561 gctcgggctg gcgttgctcg gcttcaccga gcacgccgac agcctccgcg cgatgggcga
    10621 ggacgcgctg ggcggattcg tccgcaccgg cgaagcggcg gtgcccggtc ccgggagcga
    10681 gggccaactg gtcgtcgcgg tcggcgcgcc caaggtcgag gtgccggcgc gcgatgcaca
    10741 gttcggcatc gccgtggacg cacccgcatt gttgcgcaag gtcgagatgt tccagtggaa
    10801 cgaaacccgt ttcggcggtc ggccgggcta tgagctggat tggttcgacc accccatcga
    10861 ctcgtccgca ttcgacgacg cggatcgcca cgccaatccc ggcgcgttcc cgatcaacgc
    10921 ggcccgtttc gacgcgcccg atctcaccgt cgccggcttc cacctggcgc cggagctggt
    10981 gggcatgatc cagggcccgg agtccttcga ccccgatctc tcgcacctgc cggccaacat
    11041 ggccgcaacc ttccagccgc aggacgggac gctggtgacc agcgaggatc cgggacggcc
    11101 gcgggtgggc gacctgcgca tcggctggtt gcggattgcc ccgtcggagc tgaccgtatt
    11161 cgcgcgcgat gaaaacggaa cgctcgtgcc ggcgcgcaac ccgcaaggcg aaccgatcgc
    11221 acaggtgctg atcggccgcc attcgcttac cgatgtgctc accgatgcgc cgcagccgcc
    11281 acgcttccgc tgggcacggc gcgtgctcgc ggtcctgttg gcgtgggccg gcatcacgct
    11341 gctgctcccg gaccggtatc gccacgaccg cctgttgccg ctggcggcag cggtggtgcc
    11401 gatggcgatc cttgcggcca ttttctggtt tgatgtgcgc gcctgggccg cagccggcct
    11461 ggtggcgatc acgatcctcg cggcgacctt cgccggctgg cgcgtgcgtc gcgatatccg
    11521 ggagccttga agatgctgat cgcggggttc tacgtggcgc tgggggcgct gttggtcgtg
    11581 gcgctcgcgg tgcgcgtgat gtggttgcgc aacacgcgcc gagtggggct cggcgcaggc
    11641 ggcgacgcgg tgctcgcgcg cgcgatccgg gcccatgcga atgcggccga gtacctgccg
    11701 atcgccttgc tgttgctggt gctgctcgcg ctcgagcaga cccgtccggg cctgttgcac
    11761 atgttcggaa tcgtcctgat cgtggcccgg atcctccacg ccatcggctt gtcgtcggcc
    11821 tccggccgct cgttcggccg catggtgggg atcgggctca cggtggcagt gatgctcgcg
    11881 atggcgatcc tgctgatcgt gcgcttcgcg ctggcaagtg ggtaatgcga tcgtccatta
    11941 cgctgtgcgc ctgatgtttc gccagacccg gtcaaggcgg ctgtcgtgcc gcgatcacgg
    12001 acgatcgcac gaacaagttt cgtgctcgag gaaccacagg cccgcgaaca gcttcgggtc
    12061 gatcgaataa cccttctgcg tcatcgcgtg caaccaggtg ccggcctcgc tgcgggggac
    12121 ttcgtgcacc acgatgtctt cggagtcgtc gcccccgcca tcgccgatct tgcgcagacc
    12181 gagtgcgcgc acgaagtgca tcatctcggt gctcatgccg gccgaggacg ggccggcgtg
    12241 caacggcagg acgcgcgcgc aggtccagcc ggtttcctct tcgagttcgc ggcaggccgt
    12301 ggaggcgatg tcgtcgtccg cgtgcccatt gtcgccgatc aggcccgccg gcatttcgat
    12361 cgtgcgctgg cggatcggca cccggtactg ttcgacgaac agcacgcggt cttccggcgt
    12421 caccgcgacg atgatcaccg caccctccgg attgttgcgc tcgacgaatt cccaaccccc
    12481 acgttggcgc agcgtcagcc agcgccccgc atagagcgtt tcgacaggcg cgtcggcgtc
    12541 ggccggcacg cgcggtgcgt gcgtcatgcg atcgcctcca tctgcaggat cttgagcgcg
    12601 cggatacggg tgagcggccc gaagcggaac cgctcgaaca atgccgtgag cgcggtcgcg
    12661 tcgggtgcct ggcgttgcag ccggtccgga tggcccggca ccggcgcatc cagcgcgatc
    12721 cgcgcaagcc gctgcgacag gcgcgcctgc acggcgtgga cgcgcagctt cgcggccatc
    12781 gatgcggcgc cgcgcaacct gaggaacggt acctcgtcga cacgcgcgag cagggtttcg
    12841 agatcgccga aatgcgccag cagcgcggcg gcacctttgg gtccgatgcc gggcacgccc
    12901 gggatgttgt cgatggcatc gccggtcaat gcgaggtagt cgaccacctg gtgcgcctcg
    12961 actccaagcc gtgccgatac gcccgcggcg ccccagcgtt gcccgcgcgc gtagtcccac
    13021 tgctcggcgc catcccgcag caattgcccg aaatccttgt cggccgagac gatcacgccg
    13081 cggagcccct gtccgtgcgc ggcgtgcagc acgctgccga taaggtcgtc ggcttcgtac
    13141 gcggcgtccg ccagcacggt gaggccgaag gcggcggcca gttcccggca gcattcgaac
    13201 tgtcgcttca attccggcgg cgcgggttcg cggttggcct tgtaggcggg atagatggcg
    13261 ttgcggaagg aactggtgag cgcgatgtcg aacgccgcga cggcgtgggt cgcgcgggtg
    13321 cgctcgagga aatccagcag gaaccggccg aagccgtgca cggcattgac cggctcgccg
    13381 tcgcagtcga agaacacgtt cggcatcgaa tgccaggcgc ggaacacgta catgctggcg
    13441 tcgatcaggt agagcgtcga gatcgccgcc gacgctggcg tcgcctcctt cagtccgtcc
    13501 acgcgctgac gacctcgtcg acgtcgggac ggttgcgctc gggcggttcg gactcgcggc
    13561 tgccgacgtg tatgtaggcg atcgcccgct cgccggctgc gaggcccagc agtttcgccg
    13621 ccgcagggtc gtaacacgac cagcccgtca gccactgcgc ggcgaatccg gccgcctgcg
    13681 ccgccagcaa caagttgaag ccgaggcagc ccgccgacag cagttgttcc tgcgccggaa
    13741 tcttcggatg ccgctcgtcg atgcgcgcga tcaccgcgag caccaccggc gagtgcgcga
    13801 agcgctcgcg gtccttggcg agcttcttcc cgggcacgcc gggatcgacc tgcacgtgca
    13861 gtgccgcgag ggcctcgccg tagcgcgcgc cggcgtcgcc ggtgaaggca atcagccgcc
    13921 acggcgtcag cttgccgtgg tctggtacgc ggatcgcggt ggcgatgatt tcgcgaagtt
    13981 gctccggcgt ggggccgggc ggcgccagtt gcgcagccgg cacggagcgg cggttgcgca
    14041 ggaattcgag cgcatgatca tccctcatcc ggtcagtcta gcagccgcgc cataccgctc
    14101 gacccggcca tgggctagga tgccggcatt gccgcgggag caaaccatgc cgatcaccat
    14161 taccgccttg cgcgaaaccg ccgacgggga acgccgcgtc gcggtaacac cggagaccgc
    14221 gaaaaaattc gcttcgcgcg gcttgcgggt atcgctggag catggcgcgg gcgagcgtgc
    14281 ggggttcccc gatgccgcgt atcccggcgt cgaattcgcc acgggcgatg cggtggcgca
    14341 gcaggccgac gtcgtgttgt gtgttcaacc cccggcgcag gatcgcgtcg ccggaatgaa
    14401 gcagggcgcg gcgctgatcg gccagttgca tccgcacgcc cccgccgatc ggcttgccgc
    14461 ctatgcgcag cgcgggcttg ccgcattctc gctggagctg ctgccgcgga ccacgcgtgc
    14521 gcaggcgatg gacgtgctgt cctcgcaggc ggcggtcgcg ggctaccggg cgatgctgat
    14581 cgcggccgaa tccgcgccca agcttttccc gatgatgacg accgcggccg gcacgttgcg
    14641 tccgtccaag gtgctcgtga tcggcgcggg cgtggcaggc ctgcaggcga tcgcgactgc
    14701 gcgccggctg ggcgcgatgg tcgagggctt cgacgtgcgc ccggaaaccc gcgagcagat
    14761 cgaatcgctg ggcgcgaagt tcctcgacct cggcgtcagc gccgcggggc agggcggcta
    14821 tgcgcgcgag ctcaccgccg aggaacgtgc cgcgcaacag gccaggctcg ccgaacacgt
    14881 caagacggtc gacgtgatcg tcaccaccgc cgcggtgccg gggcggccct cgcccaggat
    14941 cattccgctg tccatggtgg acggcatgaa agccggtgcg gtgatcgtgg atctggccgc
    15001 cgaaggcggc ggcaactgcg aagcgaccac gccgggcgag cgggtggagc gcgacggcgt
    15061 cacgatcatt ggcccgctga accttccgtc aggcgcgccc ttgcatgcct cggaaatgta
    15121 tgcgcgcaac ctcgcgcact tcagcgaact cgtgatcgcc gacggcgcgt ggaaaccgga
    15181 tttcgaagac gaactggtcg cgaaaacctg cgtcgcgcgc gacggcgagg ttctgttcgg
    15241 caaggcatga gaactaccgc gacggtggtc gccctcaact cccgccgctc gaccgtggcg
    15301 acgggcgggc ccggcggccg ccgcttgggt tccagcgccc gcgctgctcg ggcgtcatct
    15361 tctgccagcg ctcgcgcagc gcgcggcgct ggtccggcgg cagcgactgg aacttgcgga
    15421 atgccgcgct gactttctcg cgttcttccg gcgtgaggtt gcggaaggcg cgcgcgtttt
    15481 cgcgcagctg gtgatgctgg gccggcgtca tccctgccag gctgctcagc cgatcccgga
    15541 tctgctgctg gcgatccggc ggcaggctgg cccagcgctg cgcaaggccg gcgaggcgct
    15601 gctggcgcga ggctggcaac tggttccatt ggtcctgcat cggcgcaagc atccgctgct
    15661 ggtcggcgga tagcccggac cacgccagcg gttgcccgga ggcatccgct gcggcaggcg
    15721 ccggttgcgc cgggctggct tgcgcaagga cggtggtcgg tggggcgatg aggcacgcga
    15781 gggcgagcag ccgaagcggc tggtaccacg ggcgcatgat caacgactcc ctcgtgaacc
    15841 ggccgagctg agggccggct gggtggccag ccagcgatag aaggcgaggt cttcgaccat
    15901 ctccatctgg ccattgccga cgtccggtac gccggcttcc tgatggcctg cggattcggc
    15961 cgtgatcgcg gcgtgggtcg acgcgatcgc cgcggcctgg ccgccttggc ccccggcccg
    16021 catccagacc gccccgaggg tgaggacgca gcaggcgacg gcggtgcctg ccagtgccgt
    16081 ccagaacccg ccgaagcgat gccctgcgcc ggcgttcagc gccttgcggc gggcgagccc
    16141 caaccgcagg gcgtgatagg agtcggtgtt ctcgcaggcg ctcttgaaga gggaacgtgc
    16201 acgagcaatg acttcatcat cgctatgcaa gctgttgagg ttgttgtggt tgagatcagt
    16261 catcgccagt cctccaattg cgtgcgcagc gcatccatgg cgcgcgacag atgggccttg
    16321 acgctgcctt cgctgcagcc catcgctttc gacgtatcag cgacatccag tccttccagc
    16381 acccgcaaca ggaaggcctg ttgctggcgg cgcggcagcg ccctgatcgc cttcgacatc
    16441 gccgcatgtg cctgccgcga tgccagcatg cgcggcgggt ccggtccctg gtccggtgct
    16501 tcccaggccg gcagcgtttc ggcgtcgtcg ctcgccgcgt ggcccgtcca ggccaggacc
    16561 cggttgcgca ccgtgcgccg acgctgcagg tccgtgatcc gcctccgcag gatgccccag
    16621 aacagcggcg cccattcttc ggcgggcttg ccggcgtaat gccggaccag ccgcaacatc
    16681 gcgtcctgca cggcgtccag tgcctcgtcg ggatcgcgca acgcgatttc cgccatccgc
    16741 cacgcgcgcc gctcgacgga ggcgaggaag gcctcgaggc ttgccggcga cgatcgctcg
    16801 atgtccaggg cctgcgacgg cacggcttcg agggctgccg cggcggtcat cgcagctccg
    16861 ttggccggaa gctctttggc atccttggtc tcgcgcatcc gcacttcccc cagataacgc
    16921 atcagcgccc gcggggttga ccgccgcatg ccagccgctc cagacggtat gatggcccac
    16981 gtccgcaggg aggggaaaca tgattaccgg ttttcttgca ctgtacatct tcatgttggc
    17041 ggcgtttacc ggctacgaaa tcatcagccg ggtcccggtc atcctgcata ccccgttgat
    17101 gtcgggatcc aacttcgtgc acgggatcgt gctggtcggc gcgatggtcg cgctgggcca
    17161 tgcggaaacg ccggccgaaa tcaccatcgg agtgatcgcg gtgctgttgt ccgcgggcaa
    17221 cgtggcgggc ggttacgtcg tcaccgagcg gatgctggag atgttcaagg ccagcaaacc
    17281 cgcgtctccg cgatcggtgg agaagaagtg atggcgctcc ccgggtgggt ccccaccgtc
    17341 gtcgaagcga tctacttcat tgccgccgtc ctgttcatct ggggtctgaa acggatgagt
    17401 tcgccgcgca ccgcgcggcg cggaatcatc tgggccggga taggcatggc ggccgcgatc
    17461 ctggtgacgt tcttcctgcc gggcatgcac aacctcggtt ggatgatcgg cgcgatcgtc
    17521 ctcggggtcg ggctgtcctg gtgggtgggg cgacgcgtgc cgatgaccgc gatgccgcag
    17581 atggttgcgc tgttcaatgg catgggcggc ggtgcggcgg cggcgatcgg cgcggtcgaa
    17641 ctgatccggt tcaacgccgc gttgacgctc attccggata cgttgccggt gcaacccgaa
    17701 gcctgggcca cgatctcggg gcacctcggc gcggtcgaaa tgacgcttgc attcgtcggc
    17761 gcgctgatcg gcgcgatctc cttcaccggc tcgctgatcg cgttcgcgaa actgcagggc
    17821 tggatggacc ggcgcttcgt gttcccgctg cagcgctggg tgaacctcgc gatcttcgtc
    17881 gcggcggtcg tatgcggtgc gctggcgttg gcggcgggca tcgaaggcca tgccttcgcg
    17941 tggggcctgc tggccgcgtt cttcgtgctg tcgctggtgt tcggcgtcgc gatgacgctg
    18001 ccgatcggtg gcgccgacat gccggtcgtg atctcgatgt acaacgcctt caccggcctc
    18061 gcggtcgcat tcgaaggctt cgtgctccag aacgaggcga tgatcatcgc ggggatggtg
    18121 gtgggcgcgg ccgggacgct gctgacccag ttgatggcca aggccatgaa ccgttcgctg
    18181 ggcaacgtgc tgttcggcaa cttcggcgcg ggcagcggcg cggcgcagca ggaaatcgcg
    18241 ggcgcgctga aaccgatcga cgcggccgac gcggcggtga tgatggctta cgccgagcgc
    18301 gtcgcgatcg tcccgggcta cggcatggcg gtcgcgcagg cgcagcacaa gatctgggag
    18361 ttcgcgaagc tgctgatcga ccggggcgtc aaggtgaagt tcgcgatcca cccggtcgcg
    18421 ggccggatgc ccggtcacat gaacgtgctg ctggcggaag ccggcgtgcc ctacgacctg
    18481 atcgaggaca tggacgacat caacccggag ttcccgaaca tcgacgtcgc gctcgtgatc
    18541 ggcgccaacg acgtggtgaa cccggtcgcg cgcaccgaca agaactcacc gatctacggg
    18601 atgccgatcc tcgacgtcgc gaacgcgaag aacgtcatcg tgatcaagcg cggccgcggc
    18661 aagggctttg cgggaatcga aaacgcgctg ttctacgccg acaacacccg catgttgtac
    18721 ggcgatgggc aggccgccgc gggcgaactc gtggcgggtt tgaagaacgt ggatgcctga
    18781 gcgcggacag cgaaaaccca tttgacgcgg atttacacgg ataagagccg ataaagcagt
    18841 tctgcttgtt tgatccgcgc aaatccgcgt caaaaggcgc ttttcatcag tcgccggcgt
    18901 tgtcgctggt cgcggttcaa cggcgcgcgg gcggacgcgt gagccaagtc gccaggagcg
    18961 cggcggcgac ggccgcggcc gcgtagacca ggaccgcgat cgcgtccagg cccagttcgg
    19021 cgacctgcag ggtgaggccg aagccgccgg tgcgaaatgc caacccgaac gggaagccgg
    19081 tgaccgcggc gatccgcgcg atcgccacca ggctcaccgc gtagaacccg gccaacagca
    19141 ccgcgagcgc ggcgatcggc ccggcccacc gctcgaccag gcaaccacac agcttgagga
    19201 ccagcgcgag cggcagcgcc agcaccagga tcaaccagct cgggcgaatg cccagcacga
    19261 gcgccgcgaa tgccgcgagc gcaccggtgg cgagggtgca catcagccag ccgaaggccg
    19321 aggtcaaccc gccggcgacg aatgcaagga tggcggccgg cttgcgcgcg gacttggcgg
    19381 cggacatgga atgggcgggg tcgtccacgc gggccctttc gggaaggtcg ttcgaccgat
    19441 gatagaccag cggcatcgac cgcgccgcga cgcggtcgag cttttgccgg cgccgggacc
    19501 ttgcggaccg ggagctgacc tccatcatgg gaagcgggat gggccgttgc tatcctgtcg
    19561 ggtggttgcc cgatgcatcc acgaacttca ccgcacgccg cgagagacca tgcccggatt
    19621 cagtgccagc agcgaaccct tcaccctgca acgcgattgc catgcggtgc acgtaccgta
    19681 cggcgacgtg gtgaacctgc ccgccgggca aaccggctac atcacccagt cgctgggcgg
    19741 cagtttcacg gtgtacgtcg acggcagcct gttccggatc gatggcaagg atgccgacgc
    19801 gctcggcaag gcaccgccgg agcccctacg ggtgcccgag aacgcgggcg acgaggacgt
    19861 cgaagtcatc gtgtgggagc aattgcgcac ctgcttcgac ccggagattc cgatcaacat
    19921 cgtcgacctg ggcctggtct acgacctgtc gatggagcat ctcgaggacg ggcagcgcaa
    19981 ggtgtacgtc aagctcacgc tgacggcgcc cggttgcggc atgggcgaca tcctggtcga
    20041 agacgcgcgc accaagctgg aaatgattcc gacggtcgcc gaggcgcaga tcgacctggt
    20101 gttcgatccg ccgtggaacc aggcgatgat gtcggacgag gccaagctcc agaccggcat
    20161 gatgtaggaa caccgaaaac cggggtcaga gtcgactttc ggtcccgtgt ttgcaacctt
    20221 gcatcggctg gatcgaaagt cgactgcgac ccctcaccag ttcgccaccc gttcaagttg
    20281 ctgtccgcgc gctgaatgga ttgttgcaac gcagcgtgac cgggtatttg catttgttta
    20341 cgtttggaag gtcccatccg gtgttgaggg cgccgggcgg acaatccgca cgcgcgccgt
    20401 gtcgaccggc gtgttgcatg cgagggaata aaagggactt ggcggtttct ccgccgggtg
    20461 gggagtacgc atcgcggatt cgtatcgcgt cgcacggatg cgtgtcgcgt tgcgcatccg
    20521 tgctccttcc atccagcagg agctcaatga tgccaagcaa tcccagattg aaggctttag
    20581 tcgttgccat g
//
LOCUS       DASSNH010000150         7650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1748642, whole genome shotgun sequence.
ACCESSION   DASSNH010000150 DASSNH010000000
VERSION     DASSNH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748642"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFK00_05695"
     CDS             <1..1138
                     /locus_tag="VFK00_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7592480.1"
                     /translation="LPAFEKAHPDIHVRIQQIPLTAAHEKLLTAYAGGSLPDLAQIGN
                     TWIPEFAALHALEPLQARADGSATIREDDYFASAWATNRVEDVLYGIPWYVDTRLLFY
                     RVDLLREAGHEAPPRTWAEWQAQMDTIHTSSDGKRWGALLPTNEYDQLLALALQQHVP
                     LLRDGGRYGNFESAGFKRALAFYVGLFKRGGVPPYTNVQVTNPWQAFARGDFVFYLSG
                     PWNIGEFRKRMPAAQQSEWSTAQLPGPDGPGDSVTYGSSLVIPRSSRHKQAAWALVEY
                     LSRPDVQVKFYEQLGDLPARRSAWNSPVLKSDPKLRAFRAQLEHMRPAPQVPEWEQIV
                     NMMQELAARAIAGQLTIDEATAQMDARADTILAKRRWVLERQDE"
     gene            1328..2209
                     /locus_tag="VFK00_05700"
     CDS             1328..2209
                     /locus_tag="VFK00_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7592481.1"
                     /translation="MNRASAAWLFLAPALLVLGVFFLLPVLAGLALSLTDYDLYALAD
                     IHNLRFVGLANYWALLHRPLFWAALWHTLYFVVVGVPLSMLVSLGTALLLNSQVARMK
                     PLFRTALFAPVVTTVVAVAVIWRYLFNTQYGLVDFVLVHLGLPPIDWLGNPNWAMPMI
                     ILFAVWKNFGYNMIILLAGLQAIPADLYEAARIDGASAWRQFRHITLPALSPILVLVA
                     ILTVSGYFQLFAEPYVITQGGPLQSTVSVLYLMYDDGFKWWNMGTASAVAFMLFLVMF
                     AATALIWRFGEPRGDNA"
     gene            2206..3027
                     /locus_tag="VFK00_05705"
     CDS             2206..3027
                     /locus_tag="VFK00_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7592482.1"
                     /translation="MKRFAKPVLINGALVACAALALFPLFWMLSVSFMQPGEANNLPP
                     PMLPLHPTLDNYRELFAHAGMGRYFANSLIISTSITVLSLACNTLAGYAFAKLRFAGR
                     EKLFSAVIGALVIPAQVAMLPLFLLLKYVGLVNTYAAVILPAMASVFGIFLVRQYARS
                     IPGELLEAARVDGAGELRIFVQVVLPLLKPILVTLAVFTFLMAWNDFMWPLIALTGQQ
                     YYTLPVGLASLSREHVQDSGLMMAGAVVTVVPVLALFLALQRYYLRGLLLGSVKG"
     gene            complement(3275..4642)
                     /locus_tag="VFK00_05710"
     CDS             complement(3275..4642)
                     /locus_tag="VFK00_05710"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET7592483.1"
                     /translation="MPAEASAAVEPFPGGFLWGCATSAYQIEGSPLADGAGPSNWHRF
                     SHTPGRVRDGDTGDVACDHYRRWRDDIALMRALGMRAYRFSIAWSRVLPEGRGRVNPE
                     GLAFYDRLVDALLENGIQPMVTLYHWDLPAALDDRGGWLNPDSPGWFADYAQVVFRKL
                     DDRVGLWATINEPWVVTDGGYLHGVLAPGHRSRYEPVIAAHHLLKAHGAAVQAYRAEG
                     RNNIGIVVNLEPKYPATQGADDLAATARADAYMNRQFLDPVCRGSYPDEMKELFGDAW
                     PEWPQEDLGQIRQAIDFVGVNYYTRSVTRHDGRAWPQRASGVPQRRATRTETGWEVYS
                     QGLTDVLNWVSARYGRPPIYITENGAAFYDPPTVDGRVQDPLRVSYLRAHLRAARAAM
                     RQGVDLRGYFVWSLLDNFEWAHGYAKRFGIVHVDFATQQRTPKASARFYADVIASHGA
                     VLLPA"
     gene            4892..6007
                     /gene="ugpC"
                     /locus_tag="VFK00_05715"
     CDS             4892..6007
                     /gene="ugpC"
                     /locus_tag="VFK00_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7592484.1"
                     /translation="MATVQLDKVRKVYDNGKLAVAGADFTVADGELLVLVGPSGCGKS
                     TLLRAIAGLETITSGTIRIGGRVVDDLAPRERDIAMVFQNYALYPHMTVAENLAFGLK
                     LRGLPRAEITGRVAAAAHMLGLSELLDARPRRLSGGQRQRVALGRALVREPKVFLLDE
                     PLSNLDAKLRAAMRVEIARLHQRLAATMIYVTHDQVEAMTLGQRIVVMDAGEVQQVDT
                     PMRLYRQPANLFVAGFLGSPSMNFFRGRLGAGTSPGFDMGGASLPLGETGIASARLET
                     CRGRDVVLGLRPEDLELAGDEATTSCVQFEATLEVIEPVGNEVFLNLRHGDIALVARI
                     PPQALPTPGTRVRLQFDPSRLHAFDATDGARIDGAGG"
     gene            6194..6784
                     /locus_tag="VFK00_05720"
     CDS             6194..6784
                     /locus_tag="VFK00_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7592485.1"
                     /translation="MRKTAIVLATAAVFAAAPVFAQDTTPGADSGAFINGNVGPTNIK
                     HGINSGNDTGYAINGGYRWEVAPNFAVGPEIGYNDLGNVDVKNVFNSQPVISPGHASL
                     HGWTFGGNAKYSFTPNWYGSFRAGLYEWSGHGMSNDINPVDTHRNSQGWYGGVGVGYD
                     FTRHFGVGIGYDYYHADKANLNLTTDMVSAQAEYRF"
     gene            complement(6879..7130)
                     /locus_tag="VFK00_05725"
     CDS             complement(6879..7130)
                     /locus_tag="VFK00_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592486.1"
                     /translation="MIRPLALSFIVALLAGCSPHAPVSSDGGANANAAPASATTNALA
                     GTPMSEYGHALNRAKNVQNIVNDQAKKQAKAIDAATGGH"
     gene            7207..>7650
                     /locus_tag="VFK00_05730"
     CDS             7207..>7650
                     /locus_tag="VFK00_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7592487.1"
                     /translation="MNASPGRWRLDGQTCLITGASRGIGLACAREFAALGAQPLLVAR
                     DEAELQTACDELADEFPDCEPRLFAADVGDAEDRLAIFDWIADLDVPLSLLVNNAGGN
                     IKRATLELESAELEGVVRLNSVAAFDLCRLAYPRLAQHGNAAIVNV"
BASE COUNT         1235 a         2582 c         2535 g         1298 t
ORIGIN      
        1 cctgccggcg ttcgaaaaag cgcatcccga catccacgtc cgcatccagc agatcccgct
       61 gaccgcggcg cacgagaagc tgctgacggc ctacgccggc ggctcgctgc ccgacctcgc
      121 gcagatcggc aacacctgga tccccgagtt cgccgcgctg cacgccctgg aacccttgca
      181 agcgcgggcc gatggttccg ccacgatacg cgaggacgat tatttcgcaa gcgcctgggc
      241 caccaaccgc gtcgaggacg tactgtacgg catcccctgg tacgtcgata cgcgcctgct
      301 gttctatcgg gtggacctcc tgcgtgaagc cgggcacgaa gcaccgccgc gcacctgggc
      361 cgaatggcag gcgcagatgg acacgatcca caccagcagc gacggcaagc gttggggcgc
      421 cctgctgccg accaacgaat acgaccagtt gctggcgttg gcgctccagc agcacgtgcc
      481 gctgctccgc gacggcggcc gttacggcaa tttcgaaagt gccggtttca agcgcgcatt
      541 ggccttctac gtcggcctgt tcaagcgcgg cggcgtaccg ccgtacacca acgtgcaggt
      601 caccaatcca tggcaggcgt tcgcgcgcgg cgatttcgtg ttctatttgt cgggtccgtg
      661 gaacatcggg gaattccgca agcgcatgcc ggccgcacag caatcggaat ggtcgacggc
      721 acaactgccg ggcccggatg gccccggcga ttcggtcacc tacggatcga gcctggtgat
      781 cccgcgttcg tcgcgccaca aacaggccgc ctgggcgctg gtcgaatacc tatcgcgacc
      841 cgacgtgcag gtgaagttct acgaacaatt gggcgacctg ccggcccgcc gttcggcgtg
      901 gaacagcccg gtcctgaaaa gcgatccgaa actccgcgcc ttccgcgcgc agctcgagca
      961 catgcgtccg gcgccgcagg tgcccgaatg ggaacagatc gtcaacatga tgcaggagct
     1021 cgccgcgcgc gcgattgcag ggcaactgac gatcgacgaa gccaccgcgc aaatggacgc
     1081 ccgcgccgat acgatcctcg ccaagcggcg ctgggtgctg gagcggcaag atgagtgaac
     1141 gcaatcgaac tcgcgcgcat ctgggcgccg cgcgcaagag ccatccccct cggtcgcgct
     1201 gcgcgcgcgt tcgcttcgct ccgcccttcg cacgcgaagg gggaagacaa cctattcacc
     1261 cccttcggaa cgaagggggc cgcgccgaag gcgcgggggg atgcacgtcg cgaggaggca
     1321 ggtacatatg aaccgtgcct ccgccgcatg gctgttcctc gcgccggcgc tgctggtgct
     1381 gggggtgttc ttcctgctgc cggtgctggc ggggctggcc ttgagcctca ccgattacga
     1441 cctgtacgcg ctcgccgaca tccacaacct gcgattcgtc ggcctcgcga actattgggc
     1501 cttgctgcac cggccgctgt tctgggccgc gctctggcac acgctgtatt tcgtggtggt
     1561 gggcgtgccg ctttcgatgc tggtgtcgct cggcaccgcg ctgctcctga attcgcaggt
     1621 ggcgcgaatg aagccgctgt tccgcaccgc gctgttcgcg ccggtggtga cgacggtcgt
     1681 cgccgtcgcg gtgatctggc ggtacctctt caacacgcaa tatggccttg tcgacttcgt
     1741 gctggtgcac ctcgggctgc caccgatcga ctggctgggc aatccgaact gggcgatgcc
     1801 gatgatcatc ctgttcgcgg tctggaagaa cttcggctac aacatgatca tcctgctcgc
     1861 cgggctgcag gcgattccgg cggacctcta cgaagctgcg cggatcgacg gcgcctccgc
     1921 gtggcggcag ttccgccaca tcaccctgcc cgcactctcg ccgatcctgg tgctggtcgc
     1981 catcctcacc gtctccggct acttccagtt gttcgcggaa ccctacgtga tcacccaggg
     2041 cgggccgctg cagagcacgg tgagcgtgct gtacctgatg tacgacgacg gcttcaagtg
     2101 gtggaacatg ggcacggcct ccgcggtggc cttcatgctg ttcctcgtga tgttcgcggc
     2161 cacggccctg atctggcgtt tcggcgaacc gcgcggggac aacgcatgaa gcgctttgcc
     2221 aagccagtgc tgatcaacgg tgcgctcgtc gcatgcgcgg cgttggcgct gttcccgttg
     2281 ttctggatgc tgtcggtgtc gttcatgcag ccgggcgagg ccaacaacct gccgccgccg
     2341 atgctgccgc tgcacccgac cctcgacaac taccgcgagc tgttcgcgca cgccggcatg
     2401 ggccgctact tcgcgaacag cctgatcatt tcgacctcga tcaccgtgct ctcgctggca
     2461 tgcaacacgc tggccggcta cgccttcgcg aagctgcgat tcgccgggcg ggagaagttg
     2521 ttcagtgcgg tgatcggcgc gctggtgatc ccggcgcagg tcgcgatgct gccactgttc
     2581 ctgttgctga aatacgtcgg gctggtgaac acctacgcgg cggtgatcct gccggcgatg
     2641 gcgagcgtgt tcgggatttt cctggtgcga cagtacgcgc gcagcatccc cggggaactg
     2701 ctggaagcgg cgcgcgtgga cggtgcgggc gaactgcgga tcttcgtgca ggtggtgttg
     2761 ccgctgctga aaccgatcct ggtgacgctc gcggtgttca ccttcctgat ggcgtggaac
     2821 gacttcatgt ggccgttgat cgcgctgacc ggccagcagt actacaccct gcccgtaggc
     2881 ctcgcttcgc tctcgcgcga acacgtgcag gacagcggct tgatgatggc gggcgccgtc
     2941 gtcaccgtgg tgccggtgct ggcgctgttc ctggcgctgc agcgctatta cctgcgcggc
     3001 ctgctgctgg gaagcgtgaa agggtgagag cggcgctgtg gtaggtcggg ttgacccgcg
     3061 gcttcatgcc gggaagcccg acgtcttccc gcgagacaat gttgggcttc tcccggattg
     3121 catccgggat caacccaacc tacatgtccg ggcgtgacag ggtgagagcg gcgctgtgta
     3181 ggttggggtg acccgcggct tcatgcgggg aagcccaacc tgcacgtcgt tgcgcggctt
     3241 gccgatccgc tgcggagcgt cgcgccgaag cagatcacgc cggcagcagc acggcgccgt
     3301 ggctggcgat gacgtcggcg tagaacctgg cgctggcctt gggcgtgcgc tgctgggtcg
     3361 cgaagtccac gtgcacgatc ccgaagcgct tggcgtagcc gtgcgcccat tcgaaattgt
     3421 cgagcagcga ccatacgaag tacccccgca gatcgacgcc ctggcgcatc gcggcgcgcg
     3481 ccgcgcgcag gtgcgcacgc aggtaggaga cccgcagcgg atcctggacg cggccgtcca
     3541 cggtcggcgg atcgtagaac gccgcgccgt tttcggtgat gtagatcggc ggacggccgt
     3601 agcgggccga cacccagttc aggacgtcgg taagcccctg cgaatagact tcccagccgg
     3661 tctcggtgcg cgtcgcgcgc ctttgcggca ccccgctggc gcgctgcggc caggcgcgtc
     3721 cgtcgtggcg cgtgacgctg cgggtgtagt agttcacgcc cacgaaatcg atcgcctggc
     3781 ggatctgccc caggtcctcc tgcggccatt cgggccaggc gtcaccgaac agctccttca
     3841 tttcatccgg gtagctgccg cggcacaccg gatcgaggaa ctgacggttc atgtaggcgt
     3901 cggcgcgtgc ggtggcggcg aggtcgtcgg caccttgggt tgccgggtac ttgggctcga
     3961 ggttgacgac gatcccgata ttgttgcggc cttccgcgcg atacgcctgc accgcggcac
     4021 cgtgcgcctt cagcagatgg tgcgccgcga tcacgggctc gtatcggctg cggtggcccg
     4081 gtgcgagcac tccgtgcagg tagccgccgt cggtcaccac ccacggttcg ttgatcgtcg
     4141 cccacaggcc gacgcgatcg tcgagcttgc ggaacacgac ttgcgcatag tccgcgaacc
     4201 agccggggct gtcgggattc aaccagccgc cgcgatcgtc caacgcggcc ggaaggtccc
     4261 agtgatagag cgtgaccatc ggctggatgc cgttctcgag cagcgcatcc acgagacggt
     4321 cgtaaaacgc gaggccttcc ggattgaccc ggccgcgacc ttccggaagc acccggctcc
     4381 aggcgatgct gaagcggtag gcgcgcatgc cgagcgcgcg catcagcgca atgtcgtcgc
     4441 gccagcgccg gtagtggtcg caggcgacat cgcccgtatc accatcgcga acgcgtcccg
     4501 gcgtatgcga aaaccggtgc cagttgctcg gccctgcgcc gtcggcgagc ggcgatccct
     4561 cgatctggta cgccgaagtc gcgcagcccc agaggaaccc tcccggaaag ggttcgacgg
     4621 ccgccgaggc ttctgccggc atggatatcc ccgctggtgt aagcgattac atcggcatga
     4681 taacgtgccg cgggtggccg atgtcgaaaa cgtcgggcgg gtggctccgc cccggcgggt
     4741 gaggccaggc gggcgggtgg agcgcgtcat caacacccat ccccaccagc gctgcgcgcc
     4801 cggcctgcgg ccgcctccct gtgaaggggg cttcaagatc cctgccggcg tttggcgaag
     4861 cgaaacatca caccagccag cacttacctc catggcgacc gttcaactcg acaaggtgcg
     4921 caaggtctac gacaacggca agctcgccgt ggccggcgcc gatttcacgg tcgcggatgg
     4981 cgaactgctg gtactggtgg gtccgtccgg ctgcggcaag agcaccctgc tgcgcgcgat
     5041 cgccggcctc gaaacgatca cctccggcac gatccggatc ggcggtcggg tggtcgacga
     5101 tctcgcgccg cgcgagcgcg acatcgcgat ggtgttccag aactacgcgt tgtacccgca
     5161 catgacggtg gccgagaacc tcgcgttcgg gctgaaactg cgcgggctgc cgcgcgcgga
     5221 aatcaccggg cgcgtggcgg ccgcggcgca catgctcggc ttgtcggaat tgctggacgc
     5281 gcgcccccgc cgcctgtcgg gcggccagcg ccagcgggtg gcgctgggac gcgcactggt
     5341 ccgcgagccc aaggtgttcc tgctcgacga gccgctgtcc aacctcgacg cgaagctccg
     5401 cgcggcgatg cgggtggaga tcgcgcgcct gcaccagcgg ctcgcggcga cgatgatcta
     5461 cgtgacccac gaccaggtcg aggcgatgac gctcggccag cgcatcgtgg tgatggacgc
     5521 cggcgaggtc cagcaggtcg atacgccgat gcgcctttac cggcaaccgg cgaacctgtt
     5581 cgtcgccggt ttcctcggca gcccctcgat gaatttcttc cgcggcaggc tgggcgcggg
     5641 taccagcccg gggttcgaca tgggcggcgc gagccttccc ctcggggaga ccggcatcgc
     5701 gtcggctcga ctcgaaacgt gccgcggccg cgacgtcgtc ctcggcctgc ggcccgaaga
     5761 cctcgaactc gcgggcgacg aggcaacgac gtcgtgcgtg cagttcgaag cgacactcga
     5821 agtgatcgag ccggtcggca acgaggtgtt cctcaacctc cgtcatggtg acatcgcgct
     5881 ggtggcgcgg atcccgccgc aggcgttgcc tacgccgggc acacgcgtgc ggttgcaatt
     5941 cgatccctct cgtttgcacg cattcgatgc caccgacggt gcgaggatcg acggcgcagg
     6001 cggctgaaat tccgcgcacg attccagcgc ctgcacagcc cgcgttcatg ccggtgacgc
     6061 ctccatgaac gccatgaacc ccgcctgagg cgctagcgaa accttaacga acgttgccga
     6121 ttgcctccag tctcaatggc caagcgcgtg cgacccatgg catgcgtgaa cgacctaaca
     6181 agtggagaca atcatgcgca agactgcaat cgtcctcgcc accgccgcgg ttttcgcggc
     6241 cgcgcccgtt tttgcgcagg acacaacccc cggtgcggat agcggcgcct tcatcaacgg
     6301 caacgtcggg cccaccaaca tcaagcacgg gatcaactcg ggcaacgaca ccggctacgc
     6361 catcaacggc ggctatcgct gggaggtcgc cccgaacttc gcggtcggcc ccgaaatcgg
     6421 ctacaacgac ctcggcaacg tcgacgtcaa gaacgtattc aactcgcagc cggtgatctc
     6481 gcccggccac gcgtcgctgc acggctggac gttcggcggc aacgccaagt acagcttcac
     6541 gccgaactgg tacggttcct tccgcgccgg tctctacgag tggagcggcc acgggatgag
     6601 caacgacatc aacccggtcg acacccaccg aaattcgcag ggctggtacg gcggcgtcgg
     6661 cgtcggctac gacttcacgc ggcacttcgg cgtcggaatc ggctacgact actaccacgc
     6721 cgacaaggcc aacctgaacc ttacgaccga catggtgtcg gcgcaggccg agtatcgctt
     6781 ctagcgtgca gcggggcggg tgggtaatcc gcccgccccg tcttcggcaa cgccatcgtc
     6841 gcgcgctatc gcggccatgc gccgctccta cgagaacgtc aatggccgcc ggtcgcggca
     6901 tcgatggcct tggcctgctt cttggcctgg tcgttgacga tgttctgcac gttctttgcg
     6961 cggttgaggg cgtggccgta ttcggacatc ggcgtacccg ccagcgcgtt ggtcgtcgcg
     7021 gaagcaggcg ccgcattcgc attagcaccc ccatcggagc tgactggcgc atgcggcgag
     7081 caccccgcca acagggcgac gatgaacgac agtgcgagcg gacggatcat ggcgcgcagt
     7141 ctcgcgcgga aggcagcgcc ttgcaagctg cgctaagctc cgcgggacgc ccgacggagt
     7201 ccgcgcgtga atgcatctcc cggccgctgg cgactcgacg gccagacctg cctcatcacc
     7261 ggcgccagcc gcggtatcgg cctcgcctgc gcgcgcgaat tcgccgcgct cggcgcgcag
     7321 cccctgctgg tcgcgcgcga cgaagccgag ttgcaaaccg cgtgcgacga actcgccgac
     7381 gaattcccgg actgcgaacc gcggctgttc gcggcggacg tcggcgacgc cgaggaccgc
     7441 ctggcgatct tcgactggat cgccgacctc gacgtgccgt tgtcgctgct ggtcaacaat
     7501 gcgggcggca acatcaagcg cgcgacattg gagctcgaat ccgccgagct cgaaggcgtg
     7561 gtgcgcctca acagtgtcgc ggcgttcgac ctgtgccggc tcgcctaccc gcggctcgcg
     7621 cagcacggca acgccgcgat cgtgaacgtc
//
LOCUS       DASSNH010000151         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_381714, whole genome shotgun sequence.
ACCESSION   DASSNH010000151 DASSNH010000000
VERSION     DASSNH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381714"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFK00_05735"
     CDS             <1..229
                     /locus_tag="VFK00_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7592488.1"
                     /translation="QVVEDVNAHPNHIFCDGASRSEVVVPLVRADGTLLGVWDVDSPN
                     LARFDEDDRAGMEALCAVFMRAGADERFNAS"
     gene            complement(216..1361)
                     /locus_tag="VFK00_05740"
     CDS             complement(216..1361)
                     /locus_tag="VFK00_05740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7592489.1"
                     /translation="MQCFSQPAHAAHFRYAQVMAVFGLLAALCLPTPPALAQQPAVEL
                     HANTPAYLARIAGVHRLVYELHVTNGSDAPIELLALDVLADDDMRILHLDRRALAADA
                     NLQDDSTALAPHAQAVVYLTVDTGGDVPASVHHRLTLRQDGRESELRSPNVPVPTATG
                     PVLGPPVRGGSWVAVYSDKWPRGHRRVFIETNGHARLPGRFAIDWMKVDDQGRTLKND
                     SGLAASSYSYGAKVLAVADAYVAAVRDGVTEARLIADNQSHPHPDAAGNYVSLDLGSG
                     RFATYEHLRPGSIEVAKGERVRKGQVIAEVGFSGDSTEPHLHFHVSDSAMPLEGEGLP
                     YVMDRFRVTGRITDWQVFGSQRWTDVPPRIVEREFPESGDVVDFTTR"
     gene            complement(1440..2255)
                     /locus_tag="VFK00_05745"
     CDS             complement(1440..2255)
                     /locus_tag="VFK00_05745"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7592490.1"
                     /translation="MNAQPTPRLLEGQNALVTGANSGIGEGIARELAAQGANVAVNYV
                     VHPETAQAICDDLRKQGVEAIAVKADVSREDQVQAMFAQAVREFGTLDIVCSNAGIQR
                     DSRLTDMTLEQWNQVIGVNLTGAFLVAREAAREFTRRGMRDVSCALGKIIFTSSVHQE
                     IPWAGHCNYATTKGGIMLLMESIAQELAPQKIRVNSICPGAIKTAINRAAWATPEAEE
                     ALKTLIPCKRVGVPADIARVAAWLASDQADYIYGTSIFVDGGMTLFPGFATGG"
     gene            complement(2252..3649)
                     /locus_tag="VFK00_05750"
     CDS             complement(2252..3649)
                     /locus_tag="VFK00_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET7592491.1"
                     /translation="MASGRRMVQRSPTDEAMLDDLQRKAFRYFEHHVDPATGLVLDST
                     RPGQPVSIAAVGFALASYPVAVERGWIRRSTALEHTLAALRFFDEADQSGAQDGVGHH
                     GFFYHFLEPKTGTRAWKCELSTIDTTLLLAGAIQAAAYFDLDTARECEVRERALSIYA
                     RIDWRWALKDNNTIALGWKPETGFLVCGWCGYDESMIMHVLALAAPRFRIDPVTYDAW
                     TASYRWRHLYGEDVLYAGPLFIHQFSHIWLDLRGIRDRFMASKRTDYFENSRVATQIH
                     RQYAIRNPRKFVGYEKNCWGFSATDGPGNVVAKVAGRKRQFYDYTARGAPFGPDDGTI
                     SPWSAIASLPFAPGIVLEEVRHLEEMIGHGPQGYGFHASFNLSWPDLDTRGAHRYTLK
                     NGKQVAWVSPWHFALHQGPIVLMIENYRSEMIWNLMRQCAPIRLGLERAGFQGGWLDQ
                     ARGATTNIRKAKEPS"
     gene            complement(3652..5100)
                     /locus_tag="VFK00_05755"
     CDS             complement(3652..5100)
                     /locus_tag="VFK00_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592492.1"
                     /translation="MSTAFTCNLAASGSPLPHVWSHTVGSGRALLALRADWQAHMRRA
                     HAELGIRYVRFRGLLNDEMCTYLIECDQDVYSFFNADQVFDFLVSIGMKPFVELSFMP
                     TALQTGGDTVFFYRANVTPPRDYAKWADLVARLVKHWIARYGLEEVRSWYFEVWNEPD
                     LDAFWKGEQADYFKLYEYTANGIKDIDAQLRVGGPATANDEWIPGFLEFCELGKLPAD
                     FISTHHYPTDSLGKPDDDTQTQLSDAPRDVLIQRVRAMRRTVGGRTLCYTEWNSSSNP
                     FFEFHDEPYAAAFLFKNMLDVADLVHCYSWWTFSDIFEENYFSSEPFHGGFGLMTIHG
                     IPKPSYRAMQLLHVLGNERLPVEGSHPTVDAWAVRGNAEHVDLLLTNHAFPQHDIATE
                     TITWHVQGARKPASASLLRIDEDNGNSRQAWLDMGAPDYLDASQVAGLERSSALRASR
                     VPIRARDGGFDLEVSLPPHAIAAVRIVLARGN"
BASE COUNT          829 a         1618 c         1777 g          914 t
ORIGIN      
        1 ccaggtcgtg gaagacgtca acgcccaccc caaccacatc ttctgcgacg gcgcctcgcg
       61 ctcggaagtc gtggtgccgc tggtccgcgc cgacggcacg ttgctgggcg tgtgggacgt
      121 cgacagcccg aatctcgcgc gcttcgacga ggacgaccgg gcgggaatgg aagctttgtg
      181 cgcagtgttc atgcgcgcgg gggctgacga gcgtttcaac gcgtcgtgaa atcgaccacg
      241 tcgccggatt ccgggaactc gcgttcgaca atgcgcggcg ggacgtccgt ccagcgctgc
      301 gatccgaaaa cctgccagtc ggtgatccgc cccgtcacgc gaaagcgatc cataacgtag
      361 ggcaggcctt cgccctccag cggcatcgcc gagtcggaga cgtggaagtg caggtgaggt
      421 tcggtcgaat cgccggagaa tccgacttcg gcgatgacct ggcccttgcg gacgcgctcg
      481 cccttggcga cctcgatgct gccggggcgc aggtgttcgt aggtggcgaa tcgtccgctt
      541 ccgagatcca gtgaaacgta attgccggcc gcgtccgggt gcgggtggga ttggttgtcc
      601 gcgatcaggc gggcttccgt gacaccgtcg cgtacggcgg ccacgtacgc gtcggcgacc
      661 gccagcacct tggcgccgta ggaatacgag ctggcggcga ggccggagtc gttcttcagc
      721 gtcctgccct ggtcgtcgac cttcatccag tcgatcgcga aacggcccgg caggcgggca
      781 tgcccgttcg tctcgatgaa gacgcgacgg tgcccgcgcg gccacttgtc gctgtagacc
      841 gccacccacg aaccgccgcg cacgggcggg ccgagcactg gccccgtggc tgtcgggacc
      901 ggcacgttcg ggctccggag ttccgactcc ctgccgtcct gccgcaacgt gagtcggtgg
      961 tggacggacg ccggcacgtc gccacctgtg tcgacggtca ggtacaccac cgcctgcgca
     1021 tgcggggcca gcgcggtgct gtcgtcctgc aggttcgcgt cggcagccaa ggctctccgg
     1081 tcgagatgca ggatgcgcat gtcgtcgtcc gccaacacgt cgagtgccag caattcaatc
     1141 ggtgcatcgg agccgttggt gacgtgcaat tcgtacacca accggtggac acccgcgatc
     1201 ctggcgagat atgccggagt attggcgtgc aattcgacgg ctggctgttg ggccaatgcc
     1261 ggcggggttg gcaggcacaa cgctgcgagc aggccgaaca ccgccatgac ctgtgcgtaa
     1321 cggaaatgag ccgcgtgcgc gggttgcgag aagcattgca tgatgaatga tcgtcctggc
     1381 gtaacgggga tggtcagcgg caacccgcac tgcagcgggt tcctgggatg gtgacggatt
     1441 cacccgcccg tcgcgaagcc cggaaacagc gtcatgccgc cgtcgacgaa gatcgaagtg
     1501 ccgtagatgt aatcagcctg gtcggaggcg agccatgcgg cgacccgggc gatgtcggca
     1561 ggcacgccga cccgcttgca ggggatcagg gttttcagcg cctcctccgc ttcgggcgtg
     1621 gcccacgcgg cacgattgat cgcggtcttg atcgcgccgg ggcagatcga gttgacgcgt
     1681 atcttctgcg gcgccagctc ctgggcgatc gactccatca acagcatgat gccgcccttg
     1741 gtggtcgcgt agttgcaatg gccggcccac gggatttcct ggtgcacgga actggtgaag
     1801 atgatcttgc cgagcgcgca tgagacgtcg cgcatgcccc ggcgggtgaa ttcgcgcgcc
     1861 gcctcgcggg cgaccaggaa cgcgccggtc agattgacgc cgatcacctg gttccattgc
     1921 tcgagcgtca tgtcggtgag cctcgagtcg cgctggatgc cggcattcga gcagacgatg
     1981 tcgagcgtgc cgaattccct gacggcttgg gcgaacatcg cctgcacctg gtcctcgcgg
     2041 ctgacgtcgg ccttgaccgc gatggcctcg acgccttgtt ttcggaggtc gtcgcagatc
     2101 gcctgcgcgg tttccgggtg caccacgtag ttgacggcga cgttggcgcc ttgtgccgcg
     2161 agctcgcgcg caatgccttc gccgatgccg gagttggcgc cggtgaccag cgcgttctgt
     2221 ccctccagca gcctgggtgt cggttgagcg ttcatgatgg ctcctttgcc ttgcggatgt
     2281 tcgttgtggc gccgcgcgct tggtcgagcc agccgccctg gaatccggcg cgctcgaggc
     2341 cgagccgaat cggcgcgcac tggcgcatca ggttccagat catttcgctg cggtagttct
     2401 cgatcatcag cacgatcggt ccctggtgca gcgcgaaatg ccatggcgaa acccacgcga
     2461 cctgcttgcc gttcttcagc gtgtagcggt gcgcgcccct ggtatcgagg tcgggccacg
     2521 acaggttgaa cgacgcgtgg aagccatagc cctgcgggcc gtggccgatc atctcctcga
     2581 ggtgccgcac ctcctcgagc acgatgccgg gtgcgaacgg cagcgacgcg atcgccgacc
     2641 atggcgagat cgtcccgtcg tccggaccga acggcgcccc gcgggcggtg tagtcgtaga
     2701 actgccgttt gcggccggcg actttcgcaa ccacgttgcc tggcccgtcg gtggcagaga
     2761 agccccagca gtttttttcg tagccgacga acttgcgcgg attgcggatc gcgtactggc
     2821 ggtggatctg ggtcgccacc cggctgttct cgaagtagtc ggtgcgcttg ctggccatga
     2881 agcggtcgcg gatgccgcgc aggtccagcc agatgtgcga gaactggtgg atgaacagcg
     2941 gcccggcgta caacacgtcc tcgccgtaca ggtgccgcca gcggtagctc gcggtccagg
     3001 cgtcgtaggt gaccgggtcg atccggaatc gcggcgccgc cagcgccagc acgtgcatga
     3061 tcatcgactc gtcgtacccg caccagccgc agacgaggaa cccggtctcg ggtttccagc
     3121 cgagcgcaat ggtgttgttg tccttgagcg cccagcgcca gtcgatccgc gcgtagatcg
     3181 acaacgcacg ttcgcgcacc tcgcattcgc gggcggtgtc gagatcgaaa tacgccgccg
     3241 cctggatcgc gcccgccagc agcagcgtgg tatcgatcgt ggacagctcg cacttccagg
     3301 cacgcgtgcc ggtcttcggc tcgaggaagt ggtagaaaaa cccatggtgc ccgacgccgt
     3361 cctgcgctcc cgactggtcg gcctcgtcga agaacctgag cgcggcgagg gtgtgctcca
     3421 gcgcggtgct ccggcggatc cacccgcgct ccacggcgac cgggtagctg gccagcgcaa
     3481 aaccgaccgc ggcgatgctg accggctggc ccgggcgggt cgagtcgagg acaagaccgg
     3541 tggcggggtc gacgtggtgc tcgaaatacc ggaaggcctt gcgctggagg tcgtcgagca
     3601 tcgcctcgtc ggtcggtgag cgctgcacca tccgccgtcc cgaggccatg ctcagttccc
     3661 gcgtgccagc acgatccgca ccgcggcgat cgcgtgcggc gggaggctga cttcgaggtc
     3721 gaagccgcca tcgcgtgccc tgatcggaac gcggctcgca cgcagtgccg aggaccgttc
     3781 gagcccggca acctggcttg catcgaggta atcgggcgca cccatgtcga gccaggcttg
     3841 ccggctgttg ccattgtctt cgtcgatgcg aaggaggctc gccgaggccg gtttccgcgc
     3901 gccctgcaca tgccaggtga tggtttcggt ggcgatgtcg tgttgcggga acgcgtggtt
     3961 ggtcagcagg aggtccacgt gttcggcgtt gccgcgcacg gcccacgcgt ccaccgtcgg
     4021 gtgcgagcct tccaccggca ggcgttcgtt gccgagcacg tgcagcaact gcatcgcccg
     4081 gtagctcggc ttcggaatgc cgtggatggt catcaggccg aagccgccgt gaaacggctc
     4141 cgaggagaaa tagttttcct cgaagatgtc cgagaacgtc caccagctat agcaatgcac
     4201 gagatcggcg acgtccagca tgttcttgaa caggaacgcg gcggcgtagg gctcgtcgtg
     4261 gaattcgaag aacggattcg atgacgagtt ccactcggtg tagcaaagcg tgcggccacc
     4321 gaccgtccgg cgcatggcgc ggacacgctg gatcaacacg tcgcggggcg catcggaaag
     4381 ctgcgtttgg gtgtcgtcgt cgggcttgcc gagcgagtcg gtcgggtagt ggtgggtgga
     4441 gatgaaatct gccgggagct tcccgagttc gcagaattcc aggaagcccg gtatccattc
     4501 atcgttggcg gtggcgggac cgcccacgcg cagttgcgca tcgatgtcct tgatgccgtt
     4561 cgcggtgtac tcgtagagct tgaagtagtc ggcctgctcg cccttccaga acgcgtcgag
     4621 gtcgggctcg ttccacacct cgaagtacca cgagcgcact tcttcgaggc catagcgcgc
     4681 gatccagtgt ttgacgaggc gcgcgacgag gtcggcccac ttcgcgtagt cgcgcggcgg
     4741 ggtgacgttg gcccggtaga agaacaccgt gtcgccgccg gtctgcagcg cggtcggcat
     4801 gaacgagagt tccacgaacg gcttcatgcc gatcgaaacc aggaagtcga agacctggtc
     4861 ggcgttgaag aacgagtaca cgtcctggtc gcactcgatc aggtaggtgc acatctcgtc
     4921 gttcaggagc ccgcggaagc gcacgtagcg gatgccgagc tccgcgtgcg ccctgcgcat
     4981 gtgcgcctgc cagtcggcgc gcagcgcgag cagcgcgcgg ccgctgccga ccgtgtggct
     5041 ccagacgtgg ggaaggggac tgccggaagc ggcgaggttg caggtgaagg ctgtggacat
     5101 gcgtcattcg gctcgctgga ccggaccatc ttgtccgg
//
LOCUS       DASSNH010000152         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_973733, whole genome shotgun sequence.
ACCESSION   DASSNH010000152 DASSNH010000000
VERSION     DASSNH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973733"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFK00_05760"
     CDS             <1..1115
                     /locus_tag="VFK00_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7592493.1"
                     /translation="RARLGRPNSVRRLAAAGVDVFFGDARFAGPRALTVDGTTLRFDK
                     AVIATGARPNPPAIPGLEEAGYLTNETMFEITELPRRLLVIGGGPIGCEQAQAFCRLG
                     AHTTIVQIKPLFLEREERDAAQILSTALARDGVEVRLNTAVRSVRTENGAIRVDLVSD
                     DYHNTIEVDAILAGVGRTPNVEGLELEAAGVDHEVGKGVHVDDFLRTSNRRIHAAGDV
                     CLEDKYGHTAAVSGEMAARNALFRGRERWSKLVIPWVTFTDPEIAHVGIHVREANRRG
                     IPVKTFTVLMHEVDRAVTDSEETGFVKINVREGTDKILGATIVARHAGDMINEITLAM
                     VAKIGLRTLASVMHTYDTQAEAIQKAAKALVDREND"
     gene            complement(1512..>2790)
                     /locus_tag="VFK00_05765"
     CDS             complement(1512..>2790)
                     /locus_tag="VFK00_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET7592494.1"
                     /translation="RPGGEVFDFIESALTVDLAEVADYGRRRVLRFAMKFQQYTSPVM
                     AKGFEDTALYRYQRLACLNDVGGDPRQFGMSVAAFHHANQQRARQWPHAMLTTATHDT
                     KRGEDVRARLAAITSVPNEWGREVRHWARLNRPWKLPVQDRAAPSPADEYLLYQTLIG
                     AWLIELMEGAWDEGVAAEFVTRIQAYMTKALREAKLNSSWNNPNTDYEDATTQFVARI
                     LDRETGRLFLEAFLPLQRRVAELGAHNSLVQLVLKLTCPGVPDIYQGCELWDLSLVDP
                     DNRRPVDFRQRASLLEQVKTFDAMAPPERATQLAAWREHWQDGAIKLHVLRRLLALRA
                     AHPELFAEGDYLPIELDGVPTDSAMGFERVHGEKRLVVAAILRAGEAAGVSASLESAL
                     LTLAPGSIHVLGGQPSPPASLFEHLPVAVLLKE"
BASE COUNT          472 a          884 c          936 g          498 t
ORIGIN      
        1 ttcgtgcccg tctcggtcga ccgaactcgg tacggcggct ggcggcggcc ggggtcgacg
       61 tgttcttcgg tgatgcccgc tttgccggcc cacgtgcatt gaccgtggat ggcaccacgc
      121 tccgtttcga caaggccgtg atcgcaaccg gtgcgcgccc gaacccgccg gcgatcccgg
      181 ggctcgagga ggctggctac ctgaccaacg agaccatgtt tgaaatcacg gagcttccgc
      241 gccgcctgct ggtgatcggc ggcggtccga tcggctgcga gcaggcccag gccttttgcc
      301 ggctgggcgc gcacaccacc atcgtgcaga tcaagccatt gttcctcgag cgggaggagc
      361 gcgacgccgc gcagatcctc tcgacggcgc tcgcccgtga tggtgtcgag gtgcgtctca
      421 ataccgcggt gcgcagcgtg cgaacggaga acggcgcaat acgcgtcgat cttgtcagcg
      481 acgactacca caatacgatc gaggtcgatg cgatcctggc gggcgtcggt cgcacgccca
      541 acgtcgaagg tctcgagctg gaggcggctg gcgtcgacca cgaagtgggc aagggcgtcc
      601 acgtcgacga tttcctgcgc acttccaatc ggcgcatcca tgccgcaggc gatgtgtgcc
      661 tcgaagacaa atacggccat accgctgccg tgtcgggtga gatggccgcg cgcaatgcgc
      721 tgtttcgcgg acgcgagcgc tggagcaagc tggtcatacc ctgggttacg ttcaccgatc
      781 ccgagattgc ccatgtcggg attcacgtgc gcgaagccaa tcgtcgcggc atcccggtca
      841 agaccttcac cgtcctgatg cacgaggtcg accgggcggt caccgacagc gaagaaaccg
      901 ggttcgtgaa gatcaatgtc cgcgagggca ccgacaagat cctcggcgcg acgatcgtgg
      961 cgcgccacgc cggcgacatg atcaacgaga tcacactggc gatggtcgca aaaatcggcc
     1021 tgcggacgct tgccagcgtc atgcacacct acgacacgca ggccgaagcg atccagaagg
     1081 cggccaaggc cctcgtggat cgggaaaatg attgattccc gagtgcttcg caccatggga
     1141 tcaagcgcaa caaggccgtc catggaggtg cagaaggaca tgcccggcag cgttacggca
     1201 cacagcaagc cctgttcgct cgccgtgccc gcatggtgaa cgatgttcgg gaatgacgag
     1261 ttatggcgtt gaccagagga ttgctcgcta acgcgccgcg ataccccccc gatgtccggc
     1321 ggcgccgagt cccgtcctct gccaacgccc ttgaatcaat gcaatcaatg ccttgccccg
     1381 cgtgcgccgc ccatccctgc cgcctaattc gctattcccc aagcgggttg gtgttccggc
     1441 aacaatccca ccaggggcag tcgactgcaa ccggggtggt attgccgctc ggcgcgtggc
     1501 gcggagcggg cctactcctt cagtagcact gccacgggca ggtgctcgaa cagggacgcc
     1561 ggcggagatg gttggccgcc gaggacgtgg atggagccgg gcgccagcgt cagtagggcg
     1621 ctttcgagac tagccgagac gcccgccgct tcgccggctc gcaggattgc ggctaccacg
     1681 aggcgctttt cgccatgcac gcgctcgaac cccattgcgc tgtcggtggg aacgccatcg
     1741 agttcgatgg gcaggtaatc gccttcggcg aatagttcgg gatgcgcggc gcgcaatgcc
     1801 agcagccgcc tcaacacgtg cagcttgata gcgccgtcct gccagtgttc gcgccacgcg
     1861 gcgagttgcg tggcccgctc tggcggcgcc atcgcgtcga aggttttcac ttgttccagc
     1921 aggcttgcgc gttgcctgaa atccaccggg cggcggttgt cggggtcgac gaggctgagg
     1981 tcccacagct cgcagccctg gtagatgtcc ggcacgccgg ggcaggtgag tttcagcacg
     2041 agttgcacga ggctgttgtg ggcgccgagt tcggcgacgc ggcgctgcag cggcaggaat
     2101 gcttcgagga acaggcgacc ggtttcgcga tcgaggatgc gcgcgacgaa ttgggtggtg
     2161 gcgtcttcgt agtcggtgtt cgggttgttc caggaggagt tgagcttggc ctcgcgcagc
     2221 gccttggtca tgtaggcctg gatgcgcgtc acgaattccg cggcaacgcc ttcgtcccat
     2281 gctccttcca tcagctcgat gagccaggcg ccgatcaggg tctggtagag caggtactcg
     2341 tcggcggggc tcggcgcggc gcggtcctgc accggcagct tccagggacg gttgagcctc
     2401 gcccagtgcc gtacctcgcg gccccattcg ttcggaaccg aagtaattgc cgcgagacgc
     2461 gcgcgcacgt cctcgccgcg tttggtgtcg tgtgtcgcgg tggtcagcat cgcgtgcggc
     2521 cattgccgcg cccgttgctg gttggcgtga tggaaggccg cgaccgacat gccgaactgg
     2581 cgcggatcgc cgccgacgtc gttcaggcat gccagccgct ggtagcggta cagcgcggtg
     2641 tcttcgaaac cctttgccat taccgggctg gtgtactgct ggaacttcat cgcgaagcgc
     2701 agcacgcggc gacgtccgta atccgctact tcggcgagat cgacggtcag cgcggactcg
     2761 atgaaatcga agacttcgcc cccgggcctg
//
LOCUS       DASSNH010000153         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_585981, whole genome shotgun sequence.
ACCESSION   DASSNH010000153 DASSNH010000000
VERSION     DASSNH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585981"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFK00_05770"
     CDS             <1..1173
                     /locus_tag="VFK00_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET7592495.1"
                     /translation="QVLGRRDTPVDRPLPDQLISPPRAGLPPQSAHSDSRLDPRPLPT
                     ARTTIGRGGRVDAGSALRGAAASAPEQGGAVSTRFSTAARIIADILMRFPAAPAAIRA
                     QAPLLTTRERVHVAQLAAQLRDSIESSGLFYEAHLLRWRGGTLPLAQLLREPQMLWSG
                     RAGMPPQASFPPPPQGNAASAAAPAPPSNAMGAPATHVPVGLAAPGLPNAMAVVSPAY
                     RADGKPVASPAAGRGDAPAAQSGVPLPAPARSDLPVHPALEGVLRHQLEMLAAPVLRW
                     EGAPWAGAFMALTLQPPPQQQPEREFPGEERTPRQDGDAPWQTHLTLRLARLGEVKVN
                     LQLGAQRVALDLQAEQDAVERMNAASGTLRGRLAALGFRSVVLRIHAPDVAEAGHD"
     gene            1166..1459
                     /locus_tag="VFK00_05775"
     CDS             1166..1459
                     /locus_tag="VFK00_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET7592496.1"
                     /translation="MTEQKRRQAVALAGSDSGEAARVIAKGYGDLAEQVIAEARRHGI
                     YVHDSPELVGLLMGLDLDQRVPDRLYDVVAELLAWVAEMGAGTAASAMTRSPE"
     gene            1553..>2386
                     /locus_tag="VFK00_05780"
     CDS             1553..>2386
                     /locus_tag="VFK00_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HET7592497.1"
                     /translation="MSEESDDQEKTEPATLRRLEQARGEGQVARSRELTTFLLLGGGL
                     LGLWAMGSRLSRQLGMVMEQAFLFDRTQAFDLHVTMSRVFELGSHSLLAIAPLLLGLV
                     VLALISPLLLGGWSMSAKAMAPKLSKLNPIQGLKRMLSAQMLTELGKAIVKSLLIGGV
                     AVWFLMRHRGGLIALMGEPVMQAIPDALGLAISASGLMVLALLVVVGIDVPWQLFSHA
                     KKLRMTKEEIRRENKETEGDPHVKGRIRSQQQAMARGRMMSKVPDADVIVTNPTHYAV
                     AL"
BASE COUNT          366 a          799 c          843 g          378 t
ORIGIN      
        1 caggtattgg gccggcgcga taccccggtg gaccggccgc tgcccgacca gttgatctcg
       61 ccgccgcgcg cgggcctgcc gccgcaatcg gcgcacagcg attcgcggct ggacccgcgt
      121 ccattgccga ccgcccgcac cacgatcggc cgcggcggca gggtggatgc cgggtccgcg
      181 ctccgcggag ccgcggcctc cgcgccggaa caggggggcg ccgtcagcac ccgtttcagc
      241 acggccgccc gcatcatcgc ggatatcctg atgcggttcc cggctgcgcc tgcggccatt
      301 cgcgcacagg cgccgctgct gaccacccgc gagcgcgtgc acgtcgcgca gctggctgcg
      361 cagttgcgcg acagcatcga atccagcgga ttgttctacg aggcgcacct gctgcgctgg
      421 cgcggcggca cgctcccgct ggcgcaattg ctgcgcgagc cacagatgct ttggagcggc
      481 cgcgccggta tgccgccgca ggcttcgttt ccaccgccac cgcaaggcaa cgcagcgtcc
      541 gcggcagctc cggcgccacc ttccaacgcc atgggtgcgc cagccacgca tgttcccgtc
      601 ggtcttgccg ctcccggtct gccgaatgcg atggccgttg tttcacccgc ctatcgcgcc
      661 gacggcaagc cggtggcgag ccccgccgcg ggacgcggcg atgccccggc cgcgcaatcg
      721 ggcgtgccat tgccggcgcc cgcgcgcagc gatctgccgg tgcaccccgc actggaaggc
      781 gtgttgcggc accaactgga gatgctggcc gcgcccgtgc tgcgctggga aggcgcgccg
      841 tgggccggtg cgttcatggc gctgacgttg cagccgccac cgcagcagca gcccgaacgg
      901 gaatttccgg gcgaagaacg cacgccgcgg caggatggcg atgcgccatg gcaaacccat
      961 ttgaccttgc gccttgcccg gctgggcgag gtgaaggtga acctgcaact cggcgcgcaa
     1021 cgtgtcgcgc tggatctcca ggcggaacag gacgcggtcg agcgcatgaa cgccgcgagc
     1081 ggcacgttgc gcggacgact cgccgcgctg ggattccgga gcgtggtgtt gcgcatccac
     1141 gcccccgatg tggcggaggc cggccatgac tgagcagaaa cgccgccagg ccgtggcgct
     1201 ggccggttcc gacagtggcg aagccgcgcg cgtgatcgcg aaaggctacg gcgatcttgc
     1261 cgagcaggtg atcgccgaag cgcgtcgcca cggcatctac gtgcacgatt cgccggaact
     1321 ggtcggtttg ctgatggggc tcgatctcga ccagcgcgtg cccgaccggc tgtacgacgt
     1381 ggtcgcggaa ttgctggcct gggtggcgga aatgggtgcc ggcaccgcgg cttctgcgat
     1441 gacccgttcc cccgaatagc cgccccgatt ccgcccttat cgcgggatgg gcgaggggtc
     1501 cggcgcccca caatggcagt cgagaaactg ccgttgccgc gccgcaccca gcatgtccga
     1561 agaatccgac gatcaggaaa agaccgaacc ggccaccctt cgccgcctcg aacaggcgcg
     1621 cggggaaggg caggttgcgc gctcgcgcga attgacgacc ttcctgctgc tgggcggcgg
     1681 gctgctgggg ctgtgggcga tgggttccag gctgtcccgg cagctcggga tggtgatgga
     1741 acaggcgttc ctgttcgacc gcacgcaagc cttcgatctg cacgtgacga tgagccgggt
     1801 gttcgagctc ggttcgcaca gcctgcttgc gatcgcgccg ctgctgctcg ggctggtggt
     1861 gctcgcattg atttcgccgc tgctgctggg cggctggtcg atgtccgcca aggcgatggc
     1921 cccgaagctt tccaagctca acccgatcca gggtctcaag cggatgcttt ccgcgcagat
     1981 gctgactgaa ctcggcaagg cgatcgtgaa gtcgctgctg atcggcggcg tggcggtgtg
     2041 gttcctgatg cgccatcgcg gtggcttgat cgcgctgatg ggcgagccgg tgatgcaggc
     2101 catcccggat gcactcggct tggcgatcag cgccagcgga ttgatggtgc tggcgttgct
     2161 ggtggtggtc ggaatcgacg taccgtggca gttgttcagc cacgccaaga agctgcggat
     2221 gaccaaggaa gaaatccgtc gcgagaacaa ggaaaccgaa ggcgatccgc acgtgaaggg
     2281 ccgcatccgc tcgcagcagc aggcaatggc gcgcggacgc atgatgagca aggtgccgga
     2341 tgcggacgtg atcgtcacca atcccaccca ctacgcggtg gcgctg
//
LOCUS       DASSNH010000154         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_484529, whole genome shotgun sequence.
ACCESSION   DASSNH010000154 DASSNH010000000
VERSION     DASSNH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484529"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFK00_05785"
     CDS             <1..208
                     /locus_tag="VFK00_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592498.1"
                     /translation="DLVALAIAGTGLFTAWSCHLAWRGVDPRDVGERRNRFIAQWSVL
                     TSALFSIALIFTIVMVFIEPVCNY"
     gene            382..1587
                     /locus_tag="VFK00_05790"
     CDS             382..1587
                     /locus_tag="VFK00_05790"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7592499.1"
                     /translation="MSQLWTEWSWEPWLLVCLAAAVVPYVAGMLRMGYRRTQILGRWR
                     APSFFGGLLLLFLALMSPLDEVADSLFSAHMLQHMLLLLVIPPMLVYGRPVITWLWAF
                     DTDARRALVRGWKWSKLEAMFGFLMRPLCVWTLLTAALCCWHLPGPYDAAVRHEWLHD
                     FEHLSFLAFALAFWTIVIEPYGRRRALGYGATAVFVVSSGFVMSLIGAILVFATTPLY
                     AVHLHTTQAYGLTPLEDQQLAGIIMWIPSNLVHVAALSVVFFAWFRADERRARERVAP
                     ARATSRTALHTLVVLPLVAALLGGCAHRPTPDQIEARRGAHLIEHYGCGSCHMIPGIN
                     GADGLVGPPLIHWSRRTYIAGVLPNDPDNLAFWVQHPQRVIPGVDMPEMGIKQREAQA
                     IAAYLDAIQ"
     gene            1738..2922
                     /locus_tag="VFK00_05795"
     CDS             1738..2922
                     /locus_tag="VFK00_05795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET7592500.1"
                     /translation="MRAVTFHGKEDFRVDNVPDPKIEQPTDAIIRITSTAICGSDLHL
                     YGVLTMFLEEGDIIGHEPMGIVEEVGSEVTQIKPGDRVVVPFNISCGHCWMCDHKLFA
                     QCETTQVKSEGNGAALFGYTKLYGQVPGAQAEYLRVPQAQFGPIKVPEGPGDERFLFL
                     SDVLPTAWQAVKYADVPQGGTLTVIGLGPIGQMCCRIALQQGAERVFGIDLVPERLQM
                     ARRHGAETIDASQVDDVPAYIKEQTHGRGTDGVIDAVGMEAHGAPVEEMMQKSAGYMP
                     DKMAQKMIKKMGVDRLSALMTAIHTVRRGGTISIIGVYGGQADPMPMMTMFDKGITLR
                     MGQAHVKRWIPDILPLVLDDSDPLGTESFVTHHMPLDEAPHGYEIFQKKQDGAIKVVL
                     HP"
     gene            3020..4024
                     /locus_tag="VFK00_05800"
     CDS             3020..4024
                     /locus_tag="VFK00_05800"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7592501.1"
                     /translation="MRLKPLHQQTIVITGASSGIGLATARMAARAGARVVLASRNAEA
                     LANIASEIESEGGEVIHVAADVAHRDEVQKIADAAYKRFGGFDTWVNNAGVDLFGKLE
                     QVSEEDHRRLMDTNFWGVVNGSLVALKHLRGNGGALINLGSVESDYAIPLQGMYSASK
                     HAVKGFTDVLRIELQDAGAPVSVTLVKPWAIGTPLREHVKNYFEGEATLPPPVYDPEE
                     VANAILHAATHRRRDIFVGGAGLFTSVFSKPMPRVTDWLMKHIMWRSQFRDEPKWSHA
                     DNLHHAGDDGNVRGEWSDRTLRPSLYTRASLNPAVRYGVMAGAGAVALLLLRRSLRRR
                     "
     gene            complement(4052..4126)
                     /locus_tag="VFK00_05805"
     tRNA            complement(4052..4126)
                     /locus_tag="VFK00_05805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4092..4094),aa:Val,seq:gac)
BASE COUNT          755 a         1315 c         1393 g          748 t
ORIGIN      
        1 cgacctggtc gcgcttgcca ttgccggcac cgggttgttc acggcgtgga gttgccacct
       61 ggcgtggcgc ggggtcgatc cccgcgatgt cggcgagcgc cgcaaccgtt tcatcgcaca
      121 gtggtcggtg ctgaccagcg cacttttctc cattgccctc atattcacga tcgtgatggt
      181 cttcatcgag cccgtttgca attattgaag taacgaggga aaaggcgttt tttgaggcgg
      241 ataaaggcag atgagcggat ggagcaaaga gaatggattt gatgatcatt cgcttttatc
      301 agatgggacc gtgtcagaag acgtttgcct tcataagatt cgatccgcgt caaatgcatt
      361 tgcttcgaag gtgacggtgg aatgtcgcaa ctctggacag aatggagttg ggaaccctgg
      421 ctgctggttt gcctggccgc ggccgtggtg ccctacgtcg cggggatgct gcggatgggc
      481 taccggcgta cgcagatcct cggccgctgg cgcgcacctt cgttttttgg cgggctgttg
      541 ctgctgttcc tcgcattgat gtcgccactg gatgaggtcg ccgacagcct gttctccgcg
      601 cacatgctgc agcacatgct gctgttgctg gtgataccgc cgatgctggt gtacgggagg
      661 ccggtcatca cctggctttg ggcgttcgac acggatgccc ggcgcgccct ggtgcgtggc
      721 tggaaatggt cgaagctgga agcgatgttc gggttcctga tgcggccact gtgcgtgtgg
      781 acgctgctga ccgcggcgct ttgctgttgg cacttgccgg ggccgtacga cgccgcggtg
      841 cgccacgagt ggctgcacga tttcgagcac ttgagcttcc tcgcgttcgc gctggcgttc
      901 tggacgatcg tgatcgagcc gtacgggcgt cgtcgcgcat tgggctacgg cgcgacagcg
      961 gtgttcgtgg tgtcgtccgg cttcgtcatg agcctgatcg gcgcgatcct cgttttcgca
     1021 acgacaccgc tatacgcggt gcacctgcat accacccagg catacggatt gacgccgctc
     1081 gaggaccagc agctcgccgg catcatcatg tggatcccct ccaacctggt ccacgtggcg
     1141 gcgctcagcg tggtgttctt cgcgtggttc cgtgccgacg agcgtcgcgc gcgggagcgt
     1201 gtcgcacccg cgcgcgcgac ctcgcgcacg gccctccata cattggtggt attgccgctg
     1261 gtggcggcct tgctcggcgg ctgtgcccat cgcccgacgc ccgaccagat cgaagcgcgg
     1321 cgcggcgcgc acctgatcga gcattacggc tgcgggtctt gccacatgat ccccggcatc
     1381 aatggcgccg acggcctggt cggaccgccg ctgatccact ggagccgccg gacctacatc
     1441 gcgggcgtgc tgcccaacga cccggacaac ctggccttct gggtccagca cccgcaaagg
     1501 gtaatccccg gcgtcgacat gccggagatg ggcatcaagc agcgcgaagc acaagccatc
     1561 gcggcctacc tcgacgcgat ccagtgagac agcggcgggc aacggttgcg ttgcaaaaac
     1621 gtttgacgcg gataaaaacg gatcacacgg atggagcaaa gacatttacc tttttatcag
     1681 cttttatccg cgttgatccg catctaaaaa acgtgcaacc agggaaggta cggagccatg
     1741 cgagcagtga cgttccacgg aaaggaggat tttcgggtcg acaacgttcc ggacccgaag
     1801 atcgagcagc ccaccgacgc catcatccgg atcacctcga cggcgatctg cggatcggac
     1861 ctgcacctgt atggcgtgct gacgatgttt ttggaggagg gcgacatcat cggccacgag
     1921 ccgatgggca tcgtcgaaga ggtcggcagc gaggtcacgc agatcaagcc cggcgaccgg
     1981 gtcgtggtgc cgttcaatat ctcctgcgga cattgctgga tgtgcgacca caagttgttc
     2041 gcgcaatgcg agacgacgca ggtgaaaagc gagggcaacg gcgccgccct gttcggctac
     2101 accaagttgt acgggcaggt cccgggcgcg caggccgagt acctgcgcgt gccgcaggcg
     2161 cagttcggcc cgatcaaggt gccggaaggc cccggggacg agcgcttcct ctttctgtcg
     2221 gacgtcctgc cgacggcatg gcaggccgtg aaatacgccg acgtgccgca aggcggcacc
     2281 ctcacggtga tcgggctcgg cccgatcggc cagatgtgtt gccgcatcgc cctccagcag
     2341 ggcgcagagc gcgtgttcgg catcgacctg gtccccgaac gcctgcagat ggcgcgccgc
     2401 cacggcgcgg aaaccatcga cgccagccag gtggacgacg tgccggccta catcaaggag
     2461 caaacccacg ggcgcggtac cgacggggtg atcgatgccg tcggcatgga agcccacggc
     2521 gcaccggtgg aggaaatgat gcagaagtcg gccggctaca tgcccgacaa gatggcccag
     2581 aagatgatca agaagatggg cgtggatcgc ctctcggcgc tgatgaccgc gatccacacc
     2641 gtccgccgcg gtggcacgat ttcgatcatc ggcgtgtacg gcggacaagc cgatccgatg
     2701 ccgatgatga cgatgttcga caagggcatc accctgcgga tgggccaggc ccacgtgaag
     2761 cgctggatcc cggacatcct gccgctggtg ctcgacgaca gtgatccgct cggtaccgag
     2821 agcttcgtga cccaccacat gccgctcgac gaggcgccgc acggctacga gattttccag
     2881 aagaagcagg acggcgcgat caaggtggtg ctgcatcctt gaaccgggag cagggaacag
     2941 ggaacgggga acggggacga gggaagctcg tgacactccc tcgcccccga tccctcctcc
     3001 ctggccggca tcgaactgca tgcgactgaa acccctccac cagcaaacca tcgtgatcac
     3061 cggtgcgtcg agtggcatcg gcctcgccac ggcgcgcatg gctgcgcggg ccggggcacg
     3121 cgtggtgctc gcctcgcgca atgccgaggc gctcgcgaat atcgccagcg aaatcgagtc
     3181 ggaaggaggc gaggtcatcc acgtcgccgc ggacgttgcc caccgcgacg aagtccagaa
     3241 gatcgccgac gccgcgtaca agcgcttcgg cggtttcgac acgtgggtca acaacgccgg
     3301 cgtcgacctg ttcggcaagc tggagcaggt gagcgaggaa gaccaccggc gcctgatgga
     3361 caccaatttc tggggcgtgg tcaacggctc gctggtggcg ctgaagcacc tgcgcggcaa
     3421 cggcggcgcg ctgatcaacc tgggcagcgt cgagtccgat tacgcgatcc cgttgcaggg
     3481 catgtattcg gccagcaagc acgcggtgaa gggattcacc gatgtccttc ggatcgagct
     3541 gcaggatgcc ggcgcgccgg tgtcggtcac gctggtcaaa ccctgggcga tcggtacgcc
     3601 gctgcgcgaa cacgtcaaga actacttcga gggcgaggcg accctgccgc cgccggtcta
     3661 cgaccccgag gaagtcgcca acgcgatcct gcacgccgcc acccatcgcc ggcgcgacat
     3721 tttcgtcggc ggggcgggat tgttcaccag cgtcttcagc aaacccatgc cgcgggtcac
     3781 cgactggctg atgaagcaca tcatgtggcg gtcgcagttc cgcgacgagc cgaagtggtc
     3841 gcacgcggac aacctgcacc acgccgggga cgacggcaac gtacgcggcg aatggtcgga
     3901 ccgcaccctg cgccccagcc tttacacgcg tgcttctctg aatcccgcgg tccgttacgg
     3961 cgtgatggcc ggcgcgggcg ccgtcgcgct gttgctgttg cggcgcagcc tgcgccgtcg
     4021 atgatcgggg tttcttgcac cggagcgccg gtggtgggcg gtacagggat cgaacctgtg
     4081 acccctacca tgtcaaggta gtgctctacc gctgagctaa ccgcccactg cttgccgcgc
     4141 gatgcgaacc gcgaactata gcgggattgc gccgcccgct caattggtgg gcgttgacgc
     4201 ggcggccgcc c
//
LOCUS       DASSNH010000155        10155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_885146, whole genome shotgun sequence.
ACCESSION   DASSNH010000155 DASSNH010000000
VERSION     DASSNH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885146"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="glyA"
                     /locus_tag="VFK00_05810"
     CDS             complement(<1..1088)
                     /gene="glyA"
                     /locus_tag="VFK00_05810"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7592502.1"
                     /translation="MYSHEMRIAGYDDELATAIREERQRQEDHVELIASENYASPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCQYVDVAEALAIERLKKLFDCDYANVQPHSGSQAN
                     QAVYFALLQPGDTILGMSLAHGGHLTHGAKPNLSGKIFNAVQYGVNDQGLVDYDAVEQ
                     LAVEHKPKMIVAGFSAYSQIMDWARFRAIADRVGAYLFVDMAHVAGLVAAGVYPSPLP
                     HAHVVTSTTHKTLRGPRGGLIVANNAAMGEHAEDITKKLQSIVFPGLQGGPLMHVIAA
                     KAVAFKEALEPSFKEYQQQVVKNAKAMARTLIDRGYRIVSGGTEDHLFLVDLIGKPIT
                     GKDAEAALGKAHITVNKNAVPNDPQKPFV"
     gene            1472..3133
                     /gene="ettA"
                     /locus_tag="VFK00_05815"
     CDS             1472..3133
                     /gene="ettA"
                     /locus_tag="VFK00_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET7592503.1"
                     /translation="MQYIYTMINVSKIVPPKREIIKDISLSFFPGAKIGLLGLNGAGK
                     STVLRIMAGVDTDYQGEARPAADINIGYLAQEPNLDPDKTVREAVEEGLADILNAQKR
                     LDEIYAAYAEEGADFDALAAEQQKLENILAAGDAHTMEMQLEVAADALRLPPWDAKIG
                     PLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLHDYPGTVVAVTHDR
                     YFLDNAAEWILELDRGRGIPWKGNYSSWLEQKDERLKQEASTEKARQKAIQKELEWVR
                     SAAKGRQSKGKARLNRFEELSSLDYQRRNETNEIFIPPGERLGNEVIEFKHVSKSFGD
                     RCLIDDLSFKIPPGAIVGVIGPNGAGKSTLMKMVMGKEKPDSGEVNLGKTVQLAYVDQ
                     SRGMLNDKNNVWQEVSGGLDILKIGNFEIQSRAYIGRFNFKGSDQQKIVGTLSGGERG
                     RLHLAKTLLEGGNVLLLDEPSNDLDVETLRALEEALLEFPGCAIVISHDRWFLDRIAT
                     HIIAFEGDSHVEFFPGNYNEYEADKKRRLGEEAAQPHRVKYKKLA"
     gene            3225..4073
                     /gene="pyrF"
                     /locus_tag="VFK00_05820"
     CDS             3225..4073
                     /gene="pyrF"
                     /locus_tag="VFK00_05820"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7592504.1"
                     /translation="MGFMQSLRQAWQRNDSLVCVGLDPEPARFPAQLRDDPDAVFAFC
                     TGIVDATADLVCAFKPQIAHFAALGAEAALKRLIAHIHDRHPGVPVILDAKRGDIGST
                     ARHYAEEAFDRYQADAVTLNPYLGRDSVQPFLDRADKGVILLCRTSNPGGADFQNLAV
                     HDGDRRTLPLYQHIAATIARDWNAHGNCALVTGATFPEELGAVRALVGNIPLLVPGVG
                     AQGGDVAAVLAAGALADGTGLIVSSSRAILYAGGGLDFSEAARTAAMALRDAINRHRP
                     RADGRS"
     gene            complement(4301..4576)
                     /locus_tag="VFK00_05825"
     CDS             complement(4301..4576)
                     /locus_tag="VFK00_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET7592505.1"
                     /translation="MHPIDRAIREKRRLRFTYHGTARLVEPQCHGIGHRGTELLRAHQ
                     LEGGTQAEPLFDVAKIGDLEVLDERFDEPGPNYKRDDSAMQEIFAQL"
     gene            4659..5621
                     /locus_tag="VFK00_05830"
     CDS             4659..5621
                     /locus_tag="VFK00_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7592506.1"
                     /translation="MPSPGATTDLSRRRLLQAAGISLAAFASGALAATAPGRSRRTSK
                     LVLLGTAGGPTPKVDRSAPANAIVVGGDVYVIDCGDGVARQMVKAGLDLGRIRDVFIT
                     HQHSDHNADYGNLLLLAWETDLRTPVNTYGPPPLKRMTELFLEMDAYDIGIREKDEGR
                     PPLAPLIVPHEITRGGAVMHDDNVAVTAALVQHPLVKPAFAYRFDCPDRSIVFSGDTR
                     PSQNLAGLARGADILVHEVIDLHAIDAVVKDEAQATRLRKHLLAAHTPMSEVGRIATE
                     AGVKTLVLNHFVPGTPPIPDSVWYDAVKPHFKGRVVVGHDLMVL"
     gene            complement(5739..6215)
                     /gene="rimI"
                     /locus_tag="VFK00_05835"
     CDS             complement(5739..6215)
                     /gene="rimI"
                     /locus_tag="VFK00_05835"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7592507.1"
                     /translation="MAAVALSEPCLRPMTHADVSAVANIEAAAYEFPWSRGIFRSCLD
                     NGHHCWVLDQAGAVIGYGILSTGAGEAHILNVCVAPLHQGRGHGAYLLKRLLELARWH
                     HAGRVFLEVRPSNELAHAMYERAGFNEIGRRPNYYPARRGREDAIVMAMELLAHEV"
     gene            complement(6217..6699)
                     /locus_tag="VFK00_05840"
     CDS             complement(6217..6699)
                     /locus_tag="VFK00_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592508.1"
                     /translation="MNGAAMDAGLRERRLRALGVEPLRLRDRTVAADEVPEPAPVVTQ
                     SAPAKSDAAAAEPSPASIRRLALQPNPVELQDPAINKMYSALTEAVGKAGLQAVRVCD
                     VAEDPAAAVMVFGAAPVPEGVPAVRILRADPLALLHADRERKRLLWEQMLALGRRGQG
                     "
     gene            complement(6696..7502)
                     /locus_tag="VFK00_05845"
     CDS             complement(6696..7502)
                     /locus_tag="VFK00_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HET7592509.1"
                     /translation="MTDPLPRKSARRGIYLLPNLFTTGAMFAGFYAIVAAFLGFFPEA
                     AIAVFVAALLDGMDGRVARLTGTQSEFGVQYDSLSDLVSFGLAPSLVMYTWSLQYLRD
                     YGPVWSKLGWCAAFLYAACAALRLARFNTQVGVADKRYFQGLASPAAAGLCMSFVWSM
                     ENLGIEGQWLCFFTPFLIIIAGLLMVSRVRYFSFKTWPAGDRVPFVWIIVAVLIIVAL
                     ALNTALVLLALATVYALSGIVITLWGLRSRASRRRLRAARRHPPPTEPHP"
     gene            7642..8115
                     /locus_tag="VFK00_05850"
     CDS             7642..8115
                     /locus_tag="VFK00_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7592510.1"
                     /translation="MQPRTLFAALAVGLSIACTAGSASAQQVYKWKDSTGVVHFSQTP
                     PTNGAHFTEMKLASGSDVPSRAPAPAANPSAAAEAGQDVPDAPPPAQHGTQPDTPSNR
                     AKLCKQLGSNVSLLQGKTAVVVTGSDGKPQVMSSEAREQQLATTRAQQAQYCSGG"
     gene            8374..10080
                     /locus_tag="VFK00_05855"
     CDS             8374..10080
                     /locus_tag="VFK00_05855"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7592511.1"
                     /translation="MRLSRFHLATTREDPADAEIASHRLMLRAGMIRKLASGIYTWSP
                     LGMRVLRKVESIVREEMQNAGAIEMLMPSVQPRELWDESGRWNKYGPALLKFRDRREQ
                     EFCYGPTHEEVICEFARNELRSYKQLPATFFQIQTKFRDEIRPRFGVMRSREFIMKDA
                     YSFNLDQASMDATYRTMYEAYGRIFTRLGLKFRAVAADSGSIGGDVSHEFQVLADSGE
                     DAIAFSDGSDYAANVELAEAVAPPGGRPAPSQPLARVDTPTQKTIEEVSAFLGVPPER
                     CIKTLLVRGTDGVVALCLRGDHEVNEVKAGKLPELAGESVLASEDEIRAATGASPGFI
                     GPVGLPEVVPVIVDRAAAALADFVCGGNRDGTHYTGANWQRDARITRVADLRKVVAGD
                     PSPDGHGTLSIARGIEVGHIFQNGDKYTRTLGVTVLGEDGRDQTPVTGCYGIGVTRIV
                     AAAIEQNHDERGIAWPEPMAPWQVVICLINPKDNPAVNEAAESLYAELNARGIETALD
                     DRGLRPGAMFADLELIGIPHRIVVSERGLAAGTFEYRGRRDSESRNLDRAALLERFAA
                     AN"
BASE COUNT         1793 a         3476 c         3317 g         1569 t
ORIGIN      
        1 acgaacggct tctgcggatc gttcggcacc gcgttcttgt tgaccgtgat gtgtgccttg
       61 ccgagcgcgg cctccgcgtc cttgccggtg atcggcttgc cgatcaggtc gaccaggaac
      121 aggtggtcct cggtgccgcc cgacacgatc ctgtagccgc gatcgatcag ggtcctggcc
      181 atcgccttgg cgttcttcac gacctgctgc tggtattcct tgaacgaagg ctccagcgct
      241 tccttgaagg ccaccgcctt ggccgcgatc acgtgcatca gcggcccgcc ctgcaggccc
      301 gggaacacga tcgactgcag cttcttggtg atgtcttcgg cgtgttcgcc catcgcggca
      361 ttgttggcga cgatcaagcc gcctcgcggg ccgcgcaggg tcttgtgggt cgtcgaggtg
      421 acgacgtgcg catgcggcag cggcgacggg tacacgcccg cggcgaccag cccggccacg
      481 tgcgccatgt ccacgaacag gtacgcgccg acccggtcgg cgatcgcgcg gaagcgtgcc
      541 cagtccatga tctgcgagta cgcgctgaag ccggccacga tcatcttcgg cttgtgttcg
      601 acggcaagct gctcgaccgc gtcgtaatcg accaggccct ggtcgttcac gccgtactgg
      661 accgcgttga agatcttgcc cgagagattc ggcttggcac catgggtcag gtgcccgccg
      721 tgcgcgaggc tcatgcccaa aatggtgtcg cccggctgca gcagcgcgaa atacaccgcc
      781 tggttggcct gcgaacccga atgcggctgc acgttggcgt agtcgcaatc gaacagcttc
      841 ttcaaccgct cgatcgcgag cgcctcggcc acgtccacgt actggcagcc gccgtaatag
      901 cgcttgccgg gatagccttc ggcatatttg ttggtcagca cgctgccctg ggcttccagc
      961 acccgcgggc tcgcgtagtt ttccgaggcg atcagctcga cgtgatcctc ctggcgctga
     1021 cgctcctcgc ggatcgcggt ggccaattcg tcgtcgtaac cggcaatccg catctcgtgg
     1081 ctgtacatcg cgatcctctg gcgggcgtgt ggcagaccga agattctagc ggttccgaaa
     1141 ggtgccagcg gcgtcgactg gcgccgcgga cggttcgcgg cgataatagg cggtcaatct
     1201 gcgcggatgc agccagggat gaccagaccc ggggcggctg cgatgccggg cggccagccg
     1261 ggggcgcggt tcgtgccgga ggccggcacg cgccgaaaag acgcgccgtg atggcacgct
     1321 cctgcaccgg gaggcgtgct tcgatccgaa gggctcggaa ccgggacgcg gcgcgtccgc
     1381 ttcgaaccgc caaaacagaa tcgctgccgg gcatcgttcg cgatgaagcg cgaccggggc
     1441 caatcgacga accgagataa aaggtaaccc agtgcaatac atctacacca tgatcaacgt
     1501 gtccaagatc gtcccgccca agcgcgagat catcaaggac atttcgcttt cgttcttccc
     1561 cggcgccaag atcggcctgc tcggcctcaa cggcgcgggc aagtccacgg tgctgcgcat
     1621 catggccggc gtggacaccg actaccaggg cgaagcgcgc ccggctgccg acatcaacat
     1681 cggctacctc gcgcaggaac ccaacctgga tccggacaag accgtgcggg aagccgtcga
     1741 agaaggcctg gcggacatcc tgaatgcgca gaagcgcctc gatgaaatct acgccgccta
     1801 cgccgaggaa ggcgcggatt tcgacgcgct ggcggccgag cagcagaagc tcgagaacat
     1861 cctcgccgcg ggcgacgcgc acaccatgga aatgcaactg gaagtcgcag ccgatgcgct
     1921 gcggctgccg ccgtgggacg cgaagatcgg gccgctctcg ggcggcgaga agcgccgcgt
     1981 cgcgctgtgc cggctgctgc tttccaagcc cgacatgctg ctgctggacg agcccaccaa
     2041 ccacctcgat gccgagtcgg tcgattggct ggagcagttc ctgcacgact accccggcac
     2101 cgtggtggcg gtcacccacg atcgctactt cctcgacaac gccgccgagt ggatcctgga
     2161 actcgaccgt ggccgcggaa tcccgtggaa gggcaattac tcgtcgtggc tggaacagaa
     2221 ggacgagcgc ctgaagcagg aagcctcgac cgaaaaggcc cgccagaagg cgatccagaa
     2281 ggaactcgag tgggtgcgct cggccgccaa gggccgccag tccaagggca aggcgcgctt
     2341 gaatcgtttc gaggaactga gctcgctcga ttaccaacgg cgcaacgaga ccaacgagat
     2401 cttcatcccg ccgggcgagc gcctcggcaa cgaagtgatc gagttcaagc acgtctccaa
     2461 atccttcggc gaccgttgcc tgatcgacga cctctcgttc aagatcccgc ccggcgcgat
     2521 cgtcggcgtc atcggcccca acggcgccgg caagtccacc ctcatgaaga tggtgatggg
     2581 caaggagaag ccggattccg gggaggtcaa cctgggcaag accgtgcagc tcgcctacgt
     2641 cgaccagtcg cgcggcatgc tcaacgacaa gaacaacgtc tggcaggaag tttcgggcgg
     2701 cctcgacatc ctgaagatcg gcaacttcga gatccagtcg cgcgcctaca tcggccggtt
     2761 caacttcaag ggtagcgacc aacagaaaat cgtcggcacc ttgtccggcg gcgagcgcgg
     2821 tcgattgcat ctcgcaaaga ccttgctgga aggcggcaac gtgctgctgc tggacgagcc
     2881 gtccaacgac ctcgacgtcg aaaccctgcg cgcgctggaa gaggcgctgc tggaattccc
     2941 gggctgcgcg atcgtgatct cgcatgaccg ctggttcctc gaccgcatcg cgacccacat
     3001 catcgccttc gagggcgatt cgcacgtgga gttcttcccc ggcaactaca acgaatacga
     3061 agccgacaag aagcgccgcc tcggcgagga agccgcccag ccgcatcggg taaagtacaa
     3121 gaagctggct taactgcagg gagcagggac caggaaagcg tgacaggctc ggcatccgtc
     3181 cggatggggc ggaacgacga gcggacaggc acaccggagc gatcatgggt ttcatgcaat
     3241 ccctgcgaca ggcttggcag cgcaacgact cgctggtgtg cgtggggctc gatccggagc
     3301 ctgcgcgctt tcccgcgcag ctgcgtgacg atccggatgc ggtgttcgcc ttctgcaccg
     3361 ggatcgtcga tgccaccgcc gacctcgtgt gcgcgttcaa gccgcagata gcccacttcg
     3421 ccgcgctcgg cgccgaggct gcgctgaagc ggctgattgc acacattcac gatcggcatc
     3481 cgggtgtgcc cgtgatcctc gatgccaagc gcggcgacat cggatccacc gccaggcact
     3541 acgccgagga agcgttcgac cgctaccagg ccgatgcagt gacgctgaat ccctacctcg
     3601 ggcgggattc ggtgcaaccg ttcctcgacc gcgccgacaa gggcgtgatc ctgctgtgcc
     3661 gcacgtcgaa ccccggcggc gcggatttcc agaacctcgc cgtgcacgac ggcgatcgcc
     3721 gcacgctgcc cttgtaccag cacatcgccg cgacgatcgc gcgcgattgg aacgcgcacg
     3781 gcaactgcgc gctggtgacc ggcgcgacct tccccgagga actcggcgcc gtgcgcgcac
     3841 tggtcggcaa cattcccctg ctggtgccgg gcgtcggcgc acagggcggc gacgtagccg
     3901 cggtacttgc cgcgggcgcg ctagccgacg gcaccgggct gatcgtgagt tcttcgcgcg
     3961 cgatcctgta cgcgggcggc ggcctcgatt tctcggaagc cgcgcggacc gcggcgatgg
     4021 ccctgcgcga tgcgatcaac cgccatcggc cgcgtgccga cgggcgttcc tgagccacgc
     4081 gtaccgtagc tcgggttgat tccggactcg tccggaatat acccagcggt tgcggaaacg
     4141 cgtctgtagg ttgggttgat cccggaactt gttccgggag aagcccaaca atcgaaagcg
     4201 gacaggaacg cgacaccggt gacgtgcgga acaagcccag tcccgcgtcg ggcttcgctg
     4261 cgctcaacac caacctgcaa gagcggccgg caggcgcctc tcaaagctgg gcgaagattt
     4321 cctgcatcgc ggaatcgtcg cgcttgtagt tcggtcccgg ctcgtcgaag cgttcgtcga
     4381 gcacttccag atcaccgatc ttcgccacat cgaacagcgg ttcggcttgc gtcccgcctt
     4441 cgagctggtg tgcacgcagc aactcggtgc cgcggtgacc gatgccgtgg cattgcggtt
     4501 ccaccaggcg cgcggtgcca tggtaggtga agcgcaagcg gcgcttctcg cggatggctc
     4561 gatcgatcgg atgcatgctg cgcacgagtt tcccgctcgc ccgcctcgcc tgcaacatgg
     4621 gagtgctaga tttcccccat tcggatgcgg attcagcgat gccttccccg ggcgccacta
     4681 ccgatctttc gcgccgccgc ctgctgcagg ccgccggcat ctcgctcgcg gccttcgcga
     4741 gcggcgccct cgcggccacc gcgcctgggc gctcgcgccg aacatccaag ctcgtcctgc
     4801 tcggcaccgc gggcgggccc acgccgaagg tcgaccgctc cgcacccgcg aacgcgatcg
     4861 tggtcggcgg cgacgtttac gtgatcgact gcggcgacgg cgtcgcccgg cagatggtga
     4921 aggccggcct cgacctcgga cgcatccgcg acgtgttcat cacccaccag cactccgacc
     4981 acaacgccga ctacggaaac ctcctgttgc tcgcgtggga aaccgacctg cgcacgccgg
     5041 tcaacacata cggcccgccg ccgctgaagc ggatgaccga actgttcctg gaaatggatg
     5101 cctacgacat cggcatccga gagaaggacg agggacgccc gccattggcg cccctgatcg
     5161 tgccgcacga gatcacccgt ggcggcgcgg tgatgcacga cgacaacgtc gccgtcaccg
     5221 cggcgctggt gcaacacccg ctggtgaagc cggcgttcgc gtaccggttc gactgcccgg
     5281 atcgttcgat cgtgttctcc ggcgacaccc ggccctcgca gaacctggcc gggctcgcgc
     5341 gcggcgccga catcctggtg cacgaagtca tcgacctgca cgcgatcgac gcggtggtga
     5401 aggacgaagc ccaggccacc cggcttcgca aacatctgct cgccgcgcac accccgatgt
     5461 ccgaagtcgg gcggattgcg accgaggcgg gggtgaagac gctggtgctg aaccacttcg
     5521 tgccgggcac gccgccgatt cccgacagcg tctggtacga cgcggtgaag ccgcacttca
     5581 agggacgcgt ggtcgtcggc cacgacctga tggtcttgta gccgacggcg cgcagtgccc
     5641 gccctgcctg ctatcgcgtt cgctcggacg cgacgcgcgc aggagcggtc catggtcgcg
     5701 aaatccccag cgcacacggt cgcgcgcatg gcgcgctcct acacctcatg cgccagcagt
     5761 tccatcgcca tcacgatcgc gtcctcgcgg ccgcggcgcg ccggatagta gttcgggcgg
     5821 cggccgatct cgttgaaccc ggcgcgttcg tacatcgcgt gcgccagctc gttggacgga
     5881 cgaacctcga ggaacacgcg gcccgcgtgg tgccagcgcg ccagttcgag caggcgcttg
     5941 agcaggtaag cgccgtgccc gcggccctgg tgcaggggcg ccacgcacac gttcaggatg
     6001 tgggcttcgc ccgccccggt cgacaggatg ccgtagccga tgacggcgcc cgcctggtcg
     6061 agcacccagc aatggtggcc attgtcgagg cagctccgga agatgccgcg cgaccagggg
     6121 aattcgtagg cagccgcctc gatgttcgcg accgcggaaa catccgcgtg tgtcatcggc
     6181 cgcaggcacg gctccgacag cgccaccgcc gccatgtcag ccttgccccc ggcggccgag
     6241 cgcgagcatc tgctcccaga gcagccgctt gcgctcgcgg tcggcgtgca gcaacgcgag
     6301 cggatcggcg cgcaggatcc gaaccgccgg cacgccctcg ggcaccggcg ccgcgccgaa
     6361 caccatcacg gcggcggccg gatcctccgc cacgtcgcag acccggaccg cctgcaggcc
     6421 ggccttgccg accgcctcgg tcagtgccga atacatcttg ttgatggcgg ggtcttgcag
     6481 ctcgaccggg ttcggttgca gcgcgagccg ccggatcgaa gccggcgacg gttccgcggc
     6541 ggcggcatcg gactttgccg gcgcagactg cgtgacaacc ggggccggct ccggcacttc
     6601 atccgcggcc acggtgcggt cgcgcaagcg caatggctcg acaccgagcg cgcgcaggcg
     6661 gcgttcgcgc aggcctgcgt ccatcgccgc accgttcatg gatgcggttc cgtcggcggg
     6721 ggatgccggc gcgccgcgcg caaccgccgc cgcgaagcgc ggctgcgcag cccccacagc
     6781 gtgatcacga tcccggacaa ggcgtacacc gtcgccagtg ccagcagcac cagcgcggtg
     6841 ttcagcgcca gcgccacgat gatcagcacc gcgacgatga tccagacaaa cggtacccgg
     6901 tcaccggccg gccaggtctt gaagctgaag taacgcacac ggctgaccat cagcaaaccc
     6961 gcgatgatga tcaggaacgg ggtgaagaag cacagccatt gcccttcgat cccgaggttc
     7021 tccatcgacc acacgaagga catgcacaag cccgcggccg cggggctggc cagtccctgg
     7081 aaatagcgct tgtcggccac gccgacctgg gtgttgaagc gcgccagccg aagcgctgcg
     7141 caggccgcgt acaggaatgc cgcgcaccag cccagcttgc tccacaccgg gccgtagtcg
     7201 cgcaggtact gtagcgacca cgtgtacatc accagcgacg gcgcgagccc gaagctgacc
     7261 aggtcggaca gcgagtcgta ctggaccccg aattcgctct gggtgccggt cagccgggcc
     7321 acgcggccgt ccatgccgtc cagcagcgcg gccacgaaca ccgcgatcgc ggcttccggg
     7381 aagaacccga ggaaagcggc cacgatcgca tagaaaccgg cgaacatcgc gccggtcgtg
     7441 aacaggttcg gcagcaggta tatgccccgg cgcgcggatt ttcggggaag cggatcggtc
     7501 atgcagcgtc ggctcctggc ctcgaatcca gtgtaaccca gccatccggg gcaggttgcg
     7561 caaacgtgac gccctcacgg ttgccgccgg acccctgtgg catactcgcc gccaccgtcc
     7621 agccgcttcg ggggccttcc catgcaacct cgcaccctgt tcgccgcatt ggccgtaggc
     7681 ctgtcgatcg cctgcaccgc ggggtccgcc agcgcccagc aggtctacaa gtggaaggac
     7741 tcgaccggag tcgtgcattt ttcgcagacg ccgccgacca acggcgcgca tttcacggaa
     7801 atgaagctgg ccagcgggtc cgatgtcccg tcgcgggccc ccgccccggc cgcgaacccc
     7861 agcgccgccg cggaggctgg ccaggacgtg cccgacgcgc cgccacccgc ccaacacggc
     7921 acccaacccg acacgccctc gaaccgcgcg aagctctgca agcaactggg ttcgaatgtt
     7981 tcgctgctgc agggcaagac ggcggtggtg gtcacgggca gcgatggcaa gccgcaggtg
     8041 atgagcagcg aggcccgcga acagcagctc gcgaccacgc gcgcccagca ggcgcaatac
     8101 tgctcgggcg gctgatcctt caggcgctgc cgcaacccag cgcgacatct gcggtttcgc
     8161 gcagttccct gtacgagcgc gccatgcgcg cgaaaaatgg gtgcatcgtg attggcattc
     8221 cgatcgcggg aaaaagaagg agcaagccct tcgcgcaggc ccgctcctac gggcgctttc
     8281 tgcggggggg ccggcgccgc agaatcggtg caaatcgccg ccacgcatgg gctccggcta
     8341 caattcgccg tttcccacgg cggtaagcca ccgatgcgcc tctcccgatt ccacctcgcg
     8401 acgacccgcg aggatcctgc cgacgccgaa atcgccagcc accgcctgat gctgcgagcc
     8461 ggcatgatcc gcaagctggc gtcgggcatc tacacgtggt cgccgctcgg catgcgcgtg
     8521 ttgcgcaagg tcgagtcgat cgtgcgcgag gagatgcaaa acgccggcgc gatcgagatg
     8581 ttgatgccgt cggtgcagcc gcgcgagctg tgggacgaat ccgggcgctg gaacaagtac
     8641 ggtcccgcgc tcctgaaatt ccgcgatcgc cgcgagcagg agttctgcta cggcccgacc
     8701 cacgaggaag tgatctgcga attcgcccgc aacgaattgc gcagctacaa gcaactgccg
     8761 gccaccttct tccagatcca gaccaagttc cgcgacgaaa tccgcccccg cttcggcgtg
     8821 atgcgctcgc gcgaattcat catgaaggac gcctattcgt tcaacctgga ccaggcgtcg
     8881 atggatgcga cctaccgcac gatgtacgag gcctacgggc ggatctttac ccgcctgggc
     8941 ctgaaattcc gcgcggtcgc cgccgattcg ggatcgatcg gcggcgacgt cagccacgaa
     9001 ttccaggtgc tggccgactc cggcgaggac gcgatcgcgt tttccgacgg ctcggattac
     9061 gccgccaacg tcgagctggc cgaggcggtg gcgccgcccg gcggccgccc cgcaccctcg
     9121 cagccgctcg cgcgcgtgga caccccgacc cagaaaacca tcgaggaagt cagcgccttc
     9181 ctcggcgtgc cgccggagcg atgcatcaag accctgttgg tgcgcggcac cgacggcgtg
     9241 gtcgcgctgt gcctgcgcgg cgaccatgag gtcaacgagg tcaaggccgg caagctgccc
     9301 gagcttgccg gcgaatccgt gctggccagc gaggacgaga tccgcgcagc gaccggcgcc
     9361 tcgcccggct tcatcggacc ggtggggcta cccgaagtgg tgccggtgat cgtcgatcgc
     9421 gccgctgccg cgctcgccga tttcgtgtgc ggcggcaacc gggacgggac gcactacacc
     9481 ggcgcgaact ggcaacgcga cgcgcgcatc acgcgggttg ccgacctgcg caaggtcgtc
     9541 gccggcgatc cgtcgccgga cggacacggg acgctttcca tcgcgcgcgg aatcgaggtc
     9601 ggacacatct tccagaacgg cgacaagtac acccgcacgc tgggcgtgac ggtactcggc
     9661 gaggacggcc gcgaccagac accggtcacg ggctgctacg gtatcggcgt cacgcgcatc
     9721 gtcgccgccg cgatcgagca gaaccacgac gagcgcggca tcgcctggcc cgagccgatg
     9781 gcgccgtggc aggtggtgat ctgcctcatc aatccgaaag acaatccggc ggtgaatgaa
     9841 gccgccgaat cgctgtatgc ggaattgaat gcgcgcggca tcgaaacagc gctggacgac
     9901 cgcgggctgc gtcccggcgc gatgttcgcc gacctcgaat tgatcggcat accgcaccgg
     9961 atcgtggtca gtgaacgggg actcgccgcg ggcaccttcg aatatcgcgg acgtcgcgac
    10021 agcgagagtc gcaacctcga tcgcgccgca ttattggaaa ggtttgcggc ggcgaattga
    10081 ggccattatc ggggcgtttt gcaagcgcgc gccctttccg ccaaaatggc ggcgttgatt
    10141 ggaatcctgc gattg
//
LOCUS       DASSNH010000156         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1467749, whole genome shotgun sequence.
ACCESSION   DASSNH010000156 DASSNH010000000
VERSION     DASSNH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467749"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..544
                     /gene="ppa"
                     /locus_tag="VFK00_05860"
     CDS             2..544
                     /gene="ppa"
                     /locus_tag="VFK00_05860"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7592512.1"
                     /translation="MDLVPAGRDVPDDINVVIEIPKDAEPVKYEVDKASGAIFVDRVL
                     TTPMRYPCNYGYIPNTLSGDGDPADALVLLPMPLTPGSVIRCRAVGLLNMDDEAGVDT
                     KLVVAPGEKVFAGYAHIRDIDDVPPHWRERIAYFFEHYKDLEAGKWVKVSGWADATAA
                     REEICAAVERYRQAPEKPNF"
     gene            571..2034
                     /gene="pyk"
                     /locus_tag="VFK00_05865"
     CDS             571..2034
                     /gene="pyk"
                     /locus_tag="VFK00_05865"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7592513.1"
                     /translation="MSVSRRTRIVATLGPATDAPGALERTLGEGVDVIRLNLSHGKPE
                     DHLRRAREALAAAAKLGREVAVLADLQGPKIRVERFAEGPVQLKPGAAFTLDCRPDAP
                     AGDESRVGVSYAGLPDDVHEGDHLLLDDGLIELEVINVEGSQIRCRVVIGGKLSDRKG
                     LNRRGGGLSLDALSDKDRADIELAAELKADFLAVSFVRSAADIHEARKLLREAGGNAW
                     LVAKIERAEAIAALEEIIDASDAVMVARGDLGVEIGDAELPGLQKKIIRESLARNRVV
                     ITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSQESAAGKHPAAAVAAMSRIC
                     LGAERQFEPADEISVLTHRLDRTDQAIAMAATFLATQLPIRAIVALTESGTTAQWLSR
                     YRTSVPIYALTPSPEARRRMLLLRDVKAMAFDSRAGDPAPAVRDAVRQLFESGDLAAG
                     DRVLLTHGDHTGQGGGTNTMKLLSVGEDGIAESLRNL"
     gene            2123..2563
                     /locus_tag="VFK00_05870"
     CDS             2123..2563
                     /locus_tag="VFK00_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7592514.1"
                     /translation="MSRYILAAALLAASTASLAAVGPNIRAVPPDHINDYWVMTNTSL
                     DVDVPNSGVNLDKPTCSAVTYMIGSDGVTRDIVVQKTIPAGDLETVAKSAVKDMNYVP
                     GAINAERQPVFTYIIIPFNLPTDPAARKQITDACILKDFPQGYR"
     gene            2774..>3214
                     /locus_tag="VFK00_05875"
     CDS             2774..>3214
                     /locus_tag="VFK00_05875"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7592515.1"
                     /translation="MSIDQMEAVAQAMVAPGKGIIAIDESSGTIKKRFDAVGIPDSEE
                     HRRAYREMLLTAPGLSEHISGAILYDETIRQSTKAGVPFTQVMRDAGIIPGIKVDLGA
                     KPLAGFPGELVTEGLDGLRARLNEYVELGAQFAKWRAVITIGDDM"
BASE COUNT          588 a         1107 c         1041 g          478 t
ORIGIN      
        1 cctggatctc gtccccgccg gccgcgacgt gccggacgac atcaacgtcg tcatcgaaat
       61 ccccaaggac gccgaacccg tcaagtacga ggtggacaag gcctccggcg cgatcttcgt
      121 ggatcgcgtg ctgaccacgc ccatgcgcta cccgtgcaac tacggctaca tcccgaacac
      181 gctgtcgggc gacggcgatc ctgccgacgc gttggtgctc ctgccgatgc ccctgacgcc
      241 gggttcggtg atccgctgcc gggcagtggg cctgctcaac atggacgacg aagccggggt
      301 cgataccaaa ctggtggtcg cgccgggcga gaaggtcttc gcgggctatg cccacattcg
      361 cgacatcgac gacgtgccgc cgcactggcg cgagcgcatc gcctatttct tcgagcacta
      421 caaggacctc gaagccggca agtgggtgaa agtttccggc tgggccgatg ccacggccgc
      481 gcgcgaagaa atctgcgcgg cggtggagcg ctaccggcag gcaccggaga agccgaactt
      541 ctaggcgcgg gtctgtgcta gcgtaaggaa atgagcgttt cccgccgcac gcgcatcgtc
      601 gccacactcg gccccgccac cgacgcccct ggcgcgctgg agcgcacgct cggcgaaggg
      661 gtcgacgtca tacggctcaa cctttcgcac ggcaagcccg aagatcatct tcgtcgcgcc
      721 cgcgaagcgc tggccgcggc ggccaagctc ggccgcgaag ttgctgtcct ggccgacctg
      781 caaggcccca aaatccgcgt cgagcgcttc gccgaaggtc cggtgcagtt gaagcccggc
      841 gccgccttca ccctcgactg ccggccggac gcgcccgcag gcgacgaatc gcgggtgggg
      901 gtcagctatg ccgggctccc cgacgacgtg cacgagggcg accacctgtt gctggatgac
      961 ggattgatcg agctcgaagt gatcaacgtg gaaggctccc agatccgctg cagggtcgtg
     1021 atcggcggca agctgtccga ccgcaagggc ctgaaccgca ggggcggcgg cctttcgctg
     1081 gacgcgcttt cggacaagga ccgtgccgac atcgagctgg ccgcggaact gaaggcggac
     1141 ttcctcgccg tttcgttcgt gcgctcggcg gcggacatcc atgaagcgcg caagctgctg
     1201 cgcgaagccg gcggcaatgc ctggctggtc gccaagatcg aacgcgcgga agccatcgcc
     1261 gcactcgagg agatcatcga cgcatcggac gcggtgatgg tcgcgcgcgg cgacctcggc
     1321 gtcgaaatcg gcgacgcgga attgccgggc ctgcagaaga agatcatccg cgagtcgctg
     1381 gcgcgcaacc gggtggtgat caccgcgacg cagatgctcc aatccatggt cgaatcgccg
     1441 atcccgacgc gtgccgaggt gctggatgtg gccaacgcgg tgatcgacgg caccgatgcc
     1501 gtgatgctgt cgcaggaatc cgcagccggc aagcacccgg ccgccgcggt cgccgcgatg
     1561 agccggatct gcctcggtgc ggaacgccag ttcgagccgg ccgacgaaat ttcggtgctc
     1621 acgcatcgcc tcgaccgtac cgaccaggca atcgccatgg ccgcgacgtt cctcgccacc
     1681 cagttgccga tccgcgcgat cgtcgcgctg accgaatccg gcaccaccgc gcaatggcta
     1741 tcacgctacc gcacctcggt gccgatctac gccctgaccc ccagtcccga ggcgcgccgc
     1801 cgcatgctgc tgctgcgcga cgtcaaggcc atggcgttcg acagccgtgc cggcgacccg
     1861 gcgcccgcgg tgcgcgatgc cgtccggcaa ctgttcgaaa gcggcgacct tgcggccggc
     1921 gatcgcgtgt tgctgaccca cggcgaccac accggacagg gcggcggcac gaacaccatg
     1981 aagctgctca gcgtgggcga agacgggatc gcggagagct tgcggaactt gtgacggtct
     2041 cgggactcgg aactcgggaa aggcgtatcg ggccagagca ttcttgcgga atccgaattc
     2101 agtgcttcgt agagttccac tcatgagcag atacatcctt gccgccgccc tgctggccgc
     2161 cagcacggca agtcttgccg ccgtcggtcc caacatccgc gcggtgccgc ccgaccacat
     2221 caacgactac tgggtgatga ccaatacgtc gctggacgtc gacgtgccca acagcggggt
     2281 gaacctggac aagcccacct gctcggccgt cacctacatg atcggctcgg acggcgtgac
     2341 gcgcgacatc gtggtgcaaa agaccattcc ggccggcgac ctggagacgg tggcgaagag
     2401 cgcggtgaag gacatgaact acgtgcccgg cgcgatcaat gccgaacgcc aaccggtgtt
     2461 cacctacatc atcatcccct tcaacctgcc gaccgatccc gctgcgcgca agcagatcac
     2521 ggatgcctgc atcctcaagg attttccgca gggatatcgc tgaggagcct ctctcccgag
     2581 aggctgccgg aggcccggga cggcgcggtg aggctgtccg cgcagtgcaa catcccgctg
     2641 catgcacgga ggggcgctcg taccctcacc cggggctcca cgccacccgc tccccggagg
     2701 cagagggaac aaaggcaacg acccccgcgc tcgcctatac tcgggggtcc accgacacca
     2761 gcggaacacc gtcatgagca tcgatcaaat ggaagccgtc gcgcaggcga tggtcgcgcc
     2821 aggcaagggc atcatcgcga tcgacgaatc ctcgggcacc atcaagaagc gcttcgacgc
     2881 ggtcggcatc cccgactcgg aagagcaccg ccgcgcctac cgcgaaatgc tgctgacggc
     2941 gccgggcctc tccgagcata tctccggcgc gatcctgtac gacgaaacga tccgccagtc
     3001 caccaaggcg ggcgtaccgt tcacccaggt catgagggac gccggcatca ttcccggcat
     3061 caaggtcgac ctcggcgcca agccgctggc cggttttccg ggcgagctcg tcaccgaagg
     3121 cctggacggc ctgcgcgcgc gcctcaacga atacgtcgag ctgggcgccc agttcgccaa
     3181 gtggcgcgcg gtgatcacga tcggtgacga catg
//
LOCUS       DASSNH010000157         6470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1302104, whole genome shotgun sequence.
ACCESSION   DASSNH010000157 DASSNH010000000
VERSION     DASSNH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302104"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..980
                     /locus_tag="VFK00_05880"
     CDS             243..980
                     /locus_tag="VFK00_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7592516.1"
                     /translation="MEINTMSAKKGFFLKIGILASGAVLALFAFGPYAAANPVRINFS
                     AAGGSGYADLMLGGTNPADVVDASHPPLTITDASGMFNGVTITGIQPLNHATPPPGEV
                     LPAAYSLFSIPGYGDHDGVSYDNLFYVDGSPLICLVDGTPVYPFSGGFLDLMGIMFTL
                     DDGTFLDLWSFGVTPPGFFGPDWTGGLAYGMKLVQPNGDGFTVVDLGPSVAVVPEPGW
                     LWLFGAGLVGLFAWRRSLEKKRVPVTR"
     gene            complement(1085..2179)
                     /locus_tag="VFK00_05885"
     CDS             complement(1085..2179)
                     /locus_tag="VFK00_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET7592517.1"
                     /translation="MLLIAVALLGVAGGTAGWWLHGRRRTRVFEAAAGGSPARIAEPA
                     EPAAPLRQAERAQAPSADDIVARMYGIAFEDELDDDARVDPASPLHGKVARAAAGLLS
                     RIKPGSSYAPRRPHLLPQLLRIVSDEDSSVREIAAIISRDPAMAGNLLRISNSVLYRV
                     SPEPVEDIQRAVAMIGTDGLRQMIAVALMQPVLSGGSGPFARLPTIIWEHTLVAAVAT
                     AEHARHVERKDAFTAQMLGLLHGLGSIVVVQVARDAYARYPELAPDPAVVLRLLEQRA
                     ARTAWRLSRDWALSASIAPALDDQRRDVPLRGLTPLGRSLRIGQIAGAAAMLHRLGRI
                     QVEAVFVLMQQRLGLHEDLAARIWERLSRT"
     gene            2266..3501
                     /locus_tag="VFK00_05890"
     CDS             2266..3501
                     /locus_tag="VFK00_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7592518.1"
                     /translation="MNRSVPIEDHQASRISWRIAVMAFLGGIGGGVVFPILPTLGLRM
                     GLSAFMVGVILAANRIVRIGMNPVTGTLVDRFGGRGVIATGLFIEGVGTLGYIAALHF
                     AAPAAWFLGGRLVWGAGSSLLFVGAVAAVLAASSGANRGRLVARARSAISLGVPGGLV
                     LGGLMTDFFSADAAFLAALLLSVGSGFAALAGIPRAGGASSPADMQRRSLHGLLAVLR
                     QRRLAGIWIYAALVSFAVQGLLLATLVLLVEQRGISIAGFGAEGSAGLLLAILMLAYA
                     ATSLLIGRRLDRLHQRTQLVGASLALLVAGFVALAFTPALPLIVAALVVIGVGAGSIV
                     IPLLTLIGDLVAPGLRGRATAIYQVASDCGGTAGPIVGLILGVRLGFFAIYAGVAALF
                     VLSLPVAVALARAERRARD"
     gene            3615..4052
                     /gene="trxC"
                     /locus_tag="VFK00_05895"
     CDS             3615..4052
                     /gene="trxC"
                     /locus_tag="VFK00_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HET7592519.1"
                     /translation="MSEPLHIVCPHCQAVNRLPPERLGADPACGKCHEALFEGHPVAL
                     DAAGFDKHLARNDIPMLVDFWAPWCAPCRMMAPEFVKAAAQLEPRMRLAKVDTEAEPS
                     LGARYDIRSIPTLALFRGGREVARQAGAMQSAGIVQWARSHGA"
     gene            complement(4101..4970)
                     /locus_tag="VFK00_05900"
     CDS             complement(4101..4970)
                     /locus_tag="VFK00_05900"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HET7592520.1"
                     /translation="MMRWMALAALAWAAAWPGMTRAAPPPPVELVDVYHGGIDLSRYW
                     VSEKFDGVRGYWNGHELLTRGGTVVHVPAWFTKNWPHTPMDGELWAGYGHFARASAIV
                     RTAGADDPAWHEMSYRLFDLPGHGGNFDARVPAIRAVVAAIGDSWVVAIRQFHVRDDA
                     ELHAALKRVLAKGGEGLVLHRGDVPYRSGRGVGLLKVKPYEDAEARVIAIHPGQGRLS
                     GMMGSIEVRTPDGRRFAIGTGFSDEQRTHPPALGSWVTYRYNGLTVNGLPRFVRFLRM
                     RPGGPPPEAPANP"
     gene            5146..6345
                     /locus_tag="VFK00_05905"
     CDS             5146..6345
                     /locus_tag="VFK00_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HET7592521.1"
                     /translation="MADDAQHPEKGVPLHGRSLLADVSLPAAVVFERALAHNIAWMQR
                     FADDHGAKLAPHGKTTMTPALFGRQLKAGAWGITLATAVQCAAAFEHGASRLFMANQL
                     VGTPNMAIVAGLIERGADYYCLVDSVANAEALDEYFGGRGLVLQVLVELGVPGGRCGV
                     RSGSALDELVHAIDAAPGLALCGVEGYEGLIGGDQAVDAIRAYAQRLVDAVRTLRANG
                     SFGDRTPIVTAAGSQWFDLIAEAFDQADLRGRCFAVLRPGCYVVHDHRSYRDAMAAVK
                     KRQPDLEGQLEPALAVFAHVQSLPEAGLAVVAMGKRDISCDPDLPMPLQLHRPGQPAR
                     PLPGCRVRKVMDQHTMLAIAQHTDIRLGDIVSFGASHPCLTFDKWRQVLLVDEDLQVL
                     EAMPTFF"
BASE COUNT          979 a         2255 c         2148 g         1088 t
ORIGIN      
        1 tggatcgggt catcggacgc tccccgcgaa ctcgatcgac tatagcagtc actgcaccca
       61 atgaaagcgg attgccgcga cccttcgcgg gtgtcgccga tctttacaaa accgggggtt
      121 ttcgcatccc gtccgcacac cgaatgtcgt aaaaacaatt ggctgtcgaa tggcacactc
      181 aatgcatccg cgatgccgct acacgcccgc agaacgattg tttgcccctg ttcctgaccg
      241 acatggagat caacaccatg agtgcgaaaa agggcttctt cctcaagatc ggcatcctcg
      301 cctccggcgc ggtgctcgcg ctgtttgcgt tcggacccta tgccgccgca aacccggtcc
      361 ggatcaactt cagcgcagcg ggagggagcg ggtacgccga cctgatgctc ggaggcacca
      421 accccgccga cgtggtcgac gccagccacc cgccgctgac gatcaccgac gcgagcggca
      481 tgttcaacgg cgtgaccatc acgggcatac agcccctcaa ccatgccacg cctcccccgg
      541 gggaagtcct tccggcggcc tacagtcttt tttcgatccc gggctacggg gaccacgacg
      601 gggtttccta cgacaacctt ttttacgtcg atggctcgcc gctcatctgc ctcgtggacg
      661 gcacacccgt ctacccgttc tcgggcggtt tcctcgatct gatggggatc atgttcaccc
      721 tcgatgacgg caccttcctg gatctgtgga gtttcggagt cacccctccg ggctttttcg
      781 gacccgattg gaccggaggg cttgcctacg gaatgaaact ggtccagccg aatggcgacg
      841 gcttcacggt ggtggatctg ggcccgtccg tcgcggtggt accggaaccg ggttggctgt
      901 ggctgttcgg cgccggcctc gtcggcctgt tcgcgtggcg gcgctcgctc gagaagaagc
      961 gggtccccgt cacccgctga tcgggcaacc agcgtgacac gcaacgtgcg cggggtcgcc
     1021 ggggggtgac gcaacggggt cgtgccaagg cgcgaccccg tttgcgtatc gccggggcgg
     1081 atcctcaggt ccgagaaagc cgctcccaga tccgcgcggc caggtcctcg tgcaggccga
     1141 gccgttgctg catcaggacg aacacggcct ccacctggat cctgccgagt cgatgcagca
     1201 tcgcggctgc ccccgcgatc tggccaatgc ggagcgagcg cccgagcggg gtgagtccgc
     1261 gcaacggcac gtcgcgccgc tggtcatcca gcgccggagc gatgctcgcg gacagggccc
     1321 aatcccggga caggcgccaa gccgtgcgcg ctgcccgctg ttccagcagc cgcaggacca
     1381 cagccgggtc gggcgcgagt tccgggtagc gggcgtacgc gtcgcgcgcc acctgcacca
     1441 ccacgatgga accgaggccg tgcagcaacc ccagcatttg cgccgtaaag gcgtccttgc
     1501 gctcgacgtg gcgtgcatgc tccgcggtgg ccacggcggc caccagggtg tgttcccaga
     1561 tgatggtggg aaggcgggcg aacgggccgc tccccccgct cagcaccggt tgcatcagcg
     1621 cgaccgcgat catctgccgc aacccgtcgg tgccgatcat cgccaccgcg cgctggatgt
     1681 cttccacggg ctccgggctc acccggtaca acacgctgtt ggagatgcgc agcaggttgc
     1741 ccgccattgc gggatcgcgg ctgatgatcg ccgcgatttc acggacggag ctgtcctcgt
     1801 cgctgacgat ccggagcaat tgcggcagca ggtgcggccg gcgcggcgca tagctcgagc
     1861 cgggcttgat gcgcgacagc agccccgccg cggcgcgcgc caccttgccg tgcaatgggc
     1921 tcgccgggtc cacccgcgcg tcgtcgtcga gttcgtcttc gaaggcgatc ccatacatgc
     1981 gcgccacgat gtcgtccgca gacggcgcct gcgcgcgctc cgcctgccgc agcggtgcgg
     2041 caggttcggc gggttcggca atcctcgcag gggacccacc ggccgccgct tcgaaaaccc
     2101 gggtgcgccg gcggccatgc agccaccagc ccgcggtgcc gccggccacg cccagcagcg
     2161 cgacggctat cagcaacaag gtcgtagtca tcccccagac acgccacccc cggagccgtg
     2221 atgttacggc caaacagcgg gaaaagggga aggcataatc gtttggtgaa ccgatccgtg
     2281 cccatcgaag accaccaggc atcccggatc agttggcgaa tcgcggtgat ggcgttcctc
     2341 ggcggcatcg gcggcggcgt ggtgttcccg atcctgccga ccctggggtt gcgcatggga
     2401 ctctcggcct tcatggtcgg cgtgatcctc gccgccaacc ggatcgtgcg gatcgggatg
     2461 aacccggtca ccggcacgct ggtggaccgc ttcggtgggc gcggcgtgat cgcgacggga
     2521 ctgttcatcg agggcgtggg cacgctcggc tacatcgccg cgctgcattt cgcggcgccc
     2581 gctgcctggt tcctgggcgg gcgcctggtc tggggcgccg gctcttcgct gctgttcgtc
     2641 ggcgcggtcg ctgcggtgct cgcggccagc agcggtgcga accggggacg gctggtggca
     2701 cgcgcgcgca gcgcgatcag cctcggcgtg ccgggcgggc tggtgctcgg cggactgatg
     2761 accgacttct tttccgcgga cgcggctttc cttgccgcgc tgctcctgag cgtcggctcg
     2821 ggtttcgcgg cgctcgccgg catcccacgc gcaggtggcg cctcgtcgcc cgcggatatg
     2881 cagcgcaggt ccctgcacgg cctgctggcg gtgctgcgcc agcggcggct ggccggcatc
     2941 tggatttacg ccgcgctggt ttcgttcgcc gtgcagggcc tgcttctggc gacgctggtc
     3001 ctgctggtgg agcaacgcgg gatctccatc gccggattcg gtgccgaagg cagcgccggg
     3061 ctgctgctcg cgatcctgat gctcgcctac gccgccacat cgctgctcat cggccgccgc
     3121 ctggaccgcc tgcaccagcg cacccagctg gttggtgcga gcctcgcact gctcgtcgcc
     3181 gggttcgtgg ccctcgcctt cacaccggcg ctgccgctga tcgtggcggc gctggtggtg
     3241 atcggcgtcg gcgccggttc catcgtgatt cccctgctga cgctgatcgg tgacctcgtc
     3301 gcgccgggcc tgcgcggccg cgcaaccgcg atctaccagg tggcgtcgga ctgcggcggc
     3361 acggcgggtc cgatcgtggg attgatcctg ggcgtgcgcc tcggcttttt cgcgatttac
     3421 gcgggcgtcg cggcattgtt cgtgctgtcg cttccggtgg ccgtggcact ggcacgcgcc
     3481 gagcggcggg cacgggactg attgttcatc ccgggagcga cggcaacgct ccggtcgatc
     3541 gctttgccaa tcatttgcgc gtagcaaggc cggccccata tcgggtttgc ccccggtgaa
     3601 gtcgatgcaa agccatgagc gagccgctgc acatcgtatg tccgcactgc caagccgtga
     3661 accgcctgcc cccggaacgc cttggcgccg accccgcctg cggcaagtgc cacgaggcgt
     3721 tgttcgaggg tcatcccgtg gcgctggacg cggccggctt cgacaagcac ttggcgcgca
     3781 acgacattcc gatgctggtc gacttctggg caccctggtg cgcgccctgc cggatgatgg
     3841 cgccggaatt cgtgaaagcg gcggcgcaac tcgaacctcg catgcgcctg gcgaaggtcg
     3901 acaccgaagc cgaaccttcg ctcggcgccc gctacgacat ccgcagcatc ccgacgcttg
     3961 cgttgttccg cggcggccgc gaagtggcgc gccaggctgg cgccatgcaa agcgcaggca
     4021 tcgtgcagtg ggcgcgaagc cacggcgcgt gatgccatgc gctggagcgg gcaaccggct
     4081 gccggaatgc cgcgctacga tcacggattc gccggggctt ccggtggcgg tccgccgggt
     4141 cgcatccgca ggaagcgcac gaaacgcggc agaccgttga cggtcaatcc gttgtatcga
     4201 taggtgaccc acgagcccag cgccggcggg tgcgtgcgct gctcgtcgga aaaacccgtt
     4261 ccgatcgcga agcggcgccc gtcgggcgtg cgtacctcga tggaccccat catgcccgag
     4321 aggcggccct ggcccgggtg gatggcgatc acccgcgcct cggcgtcttc atagggcttc
     4381 accttgagca gcccgacccc gcgccccgaa cgatacggga cgtcgccacg gtgcagcacc
     4441 aacccctcgc cgcccttggc cagcacgcgc ttcaacgccg cgtgcaactc ggcatcgtcg
     4501 cggacgtgga actggcggat ggcgaccacc caggaatccc cgatcgcggc gacgaccgcg
     4561 cgaatggcgg gaacgcgcgc gtcgaagttc ccaccgtgcc ccggcagatc gaacagacga
     4621 tagctcatct cgtgccatgc gggatcgtcc gccccggccg tgcgcacgat cgcggaagcc
     4681 ctcgcgaagt gcccgtagcc ggcccacagc tcgccgtcca tgggggtgtg cggccagttc
     4741 ttcgtgaacc aagccggcac gtgcaccacc gtgcccccgc gcgtcaacaa ctcgtggcca
     4801 ttccagtaac cgcgcacgcc atcgaatttc tcgctgaccc agtagcggga caagtcgata
     4861 ccgccgtggt acacatcgac cagttcgacc ggtggcggcg gcgcggcgcg agtcatcccc
     4921 ggccacgccg cggcccatgc cagcgcggcc agcgccatcc aacgcatcat cgaacgcatc
     4981 gtctcgctcc gcccatcacc agcggggatt gtcggcacgg gtgacgggcg gcgctgtcgg
     5041 tcgcggtacc gagcgccatg cggggcggtc ctggagatct gtcggcgaat ccctacgcga
     5101 gcccgttcgc ctatgcttgt gcgcctgcgt tccaggaaat cgtgcgtggc cgacgacgcg
     5161 caacacccgg aaaagggcgt cccgctccat ggccggtcgc tgctcgcgga cgtgagcctc
     5221 ccggccgcgg tggtattcga gcgcgcgttg gcccacaaca tcgcgtggat gcaacgcttc
     5281 gccgacgacc acggcgcgaa gctcgcgccg cacggcaaga ccacgatgac gcctgcccta
     5341 tttggccgcc agctcaaggc cggcgcctgg ggcatcacgc tcgcgaccgc ggtccagtgc
     5401 gccgcggcct tcgagcacgg cgcctcgcgt ctcttcatgg ccaaccaatt ggtcggcacg
     5461 cccaacatgg cgatcgtcgc cggactgatc gagcgcggcg cagattacta ctgcctggtc
     5521 gacagcgtcg cgaatgccga ggctttggac gagtatttcg gaggacgagg tctcgtcctg
     5581 caggtactgg tcgaactcgg cgtgccgggt ggccgttgtg gcgtgcgcag cgggtcggca
     5641 ctcgatgagc tcgtgcatgc catcgacgcg gccccgggcc tcgcactctg cggtgtcgaa
     5701 ggctacgaag ggctgatcgg tggcgaccag gccgtcgatg ccattcgcgc gtacgcgcag
     5761 cggctcgtgg acgccgttcg cacgctccgc gccaacggat cattcggcga tcgcaccccg
     5821 atcgtcaccg cggcgggatc gcaatggttc gacctgatcg cggaagcttt cgaccaggcg
     5881 gacctgcgcg gtcgctgctt cgcagtcctc aggcccggtt gctacgtcgt ccatgaccac
     5941 cgttcctacc gcgacgcgat ggccgcggtg aagaagcgcc aaccggatct cgaagggcaa
     6001 ctcgagccgg cactggcggt gttcgcgcac gtgcagtcgc tgcccgaagc cggcctggcg
     6061 gtggtcgcca tgggcaagcg cgacatctcg tgcgatccgg accttccgat gccgctgcaa
     6121 ctgcaccggc caggccaacc cgcgcggccg ctgccgggtt gccgtgtccg gaaggtcatg
     6181 gaccagcaca ccatgctggc gatcgcccaa cacaccgaca tccgcctcgg cgacatcgtg
     6241 tctttcggcg cttcgcatcc gtgcctcacc ttcgacaagt ggcgacaggt gctgctcgtc
     6301 gatgaagacc tgcaggtgct cgaagcgatg ccgacgtttt tctgaatgcc ccgaaaacga
     6361 tggtaggctc gaccttcgaa ccatgcggcg tgcaatgcaa cgtgcacctc tcctcgtgac
     6421 attatcggga cgccatgacc aagcatcttc gaatccttgc ctgcctgttc
//
LOCUS       DASSNH010000158         7339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1262546, whole genome shotgun sequence.
ACCESSION   DASSNH010000158 DASSNH010000000
VERSION     DASSNH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262546"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFK00_05910"
     CDS             <1..415
                     /locus_tag="VFK00_05910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET7592522.1"
                     /translation="YDADFPPVAREFKQRIADADAILFITPEYNRSIPGGLKNAIDWA
                     SRPWGKNSFARKPSAIIGTSPGAIGTAIAQQSLRGVLCFCNSPLMNAVEAYIQFTPNL
                     IDDDGEVGNEATTEFLRNYMAEFSAFIRRVKMALS"
     gene            complement(469..1095)
                     /locus_tag="VFK00_05915"
     CDS             complement(469..1095)
                     /locus_tag="VFK00_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HET7592523.1"
                     /translation="MTHILVAVLDLGGTFVFAISGAVAAVKHRLDIFGVMVLAFAAGN
                     VGGITRDLLIGAVPPAAIASLNYLGVSFLAGLIVFFQDRLVTRHNNVVLWFDAVGLAF
                     FAVAGAEKALVHGMNPVMAALLGMLTGIGGGMLRDVLVMQVPTVLRSDLYALAALVGA
                     GIVVGGHVLHIHAVATTVVGGLACFALRYMAIRHGWHLPHARPSEDEP"
     gene            complement(1152..2420)
                     /locus_tag="VFK00_05920"
     CDS             complement(1152..2420)
                     /locus_tag="VFK00_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7592524.1"
                     /translation="MNVSTAGAPDPVLGGLDRLLPDLKALYKDVHSHPELSMQESRTS
                     GLAADRLRAAGFEVTTGIGNTGVVGLLRNGDGPTVMLRADMDALPVEEATGVAYASTV
                     TASDPDGKSVPVMHACGHDMHVTWLAGATALMAQARDAWHGTLMAVFQPAEETAAGAK
                     AMIDDGLFKRFPKPDVVLGQHVMVGPAGALGGRTGAITSAADSLQIRLFGRGAHGSMP
                     QSSIDPVVMAAAVVMRLQTIVSREVAAAESAVVTVGALQAGTKENVIPDEAIIKLNVR
                     TFDEGVRKRVLAAIERIVNAEAMASGAPKPPEITPLDRYPLASNDAGASKRVVEAFRG
                     YFSPARVHETGPASASEDFGSFGAEWGVPSVFWFVGGTDPDVYAQAKASNRLNEIPTN
                     HNPRFLPVIHPTLETGVEALVVASRAWLSA"
     gene            complement(2567..3457)
                     /locus_tag="VFK00_05925"
     CDS             complement(2567..3457)
                     /locus_tag="VFK00_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7592525.1"
                     /translation="MDDRDAFARPPQRILLATDLEAHSDRAIDRAAMLARQWQAKLHV
                     IHVLQPGPPDNWWISGDDGASADGEAERVERQLRRDLHDAPDDLVVHIAEGEPADRIL
                     EIAEREGCGLVVVGTRGPIFADLVGRSTTVQLLRRSPWPLLIVKARPHGAYRKVLVGT
                     DFTPESLDGLETAVAWFADADFTLMHALDVPHRSLLLDADRGQAFARLEREAMQSFVD
                     GARLPHGTGRRLQTEIEYGPPDLLLRRRALANDIDLTVIGALKRGLAFRIVVGGNATR
                     IVEAAPGDVLTVRAPPSGDT"
     gene            complement(3692..4603)
                     /locus_tag="VFK00_05930"
     CDS             complement(3692..4603)
                     /locus_tag="VFK00_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7592526.1"
                     /translation="MDYNLADIRAFVQVVDDGSITAAANRLGVAKSSLSARIARLEAA
                     LHVQLLHRTARGAAVTDAGRRFHARMLDLLGGIQQAADEAAGGDGALAGNLRITAPVT
                     FGATYLGRSVFGFAARHPGLAITLSLDDRYVDLAGEGFDMGVRIGRLADSSLMARRIA
                     RSPRVLCCSPAYAAGHALPKRIEDIQQHPCIAYGNASSTPFWQFAPARRGGEPRQIAV
                     RGRVYTNNGEGMRDAAMAGLGLAILPLFIAGPALAAGQLQVVLPRTPPTEDAIHAVYP
                     PTRYVSRAVRRLIDTLVDRFREFEWSR"
     gene            4726..5436
                     /locus_tag="VFK00_05935"
     CDS             4726..5436
                     /locus_tag="VFK00_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7592527.1"
                     /translation="MYSIRPAGERGQSRMGWLDSRHTFSFGNYHDPIHMGFGALRVIN
                     EDRVQPGQGFGTHSHRDMEILSWVLDGALEHRDSMGTHGVIRPGELQRMSAGTGVTHS
                     EFNASASEPVHFLQIWLIPEREGLAPSYEQRAFAPADLTDRLHLIASHDGRDDSIEVH
                     QDVNLYASRLAPDATVSFRPAAGRSLWLHVAKGRVQVDDRELKAGDAASWTAAPDALD
                     IRATEPSEVLLFDLAPTQ"
     gene            5584..5994
                     /locus_tag="VFK00_05940"
     CDS             5584..5994
                     /locus_tag="VFK00_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7592528.1"
                     /translation="MKTLTNSADLLGRLFLVLLFVVAGYGKIGGYAGTAAYMAANGVP
                     GLLLPLVIALELGGGILVLLGWHTRIMSLLLAGFTLLALLLFHMPMTAENQIVFLAEL
                     GVAGGFLVLAAHGPGAWSLDRWLARRAPAKAVTA"
     gene            complement(6122..>7339)
                     /locus_tag="VFK00_05945"
     CDS             complement(6122..>7339)
                     /locus_tag="VFK00_05945"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET7592529.1"
                     /translation="FSTVAGEMGSPDTWRDVRGFSMKFYTSEGNYDIVGNNTPVFFLR
                     DPMKFPHFIRSQKRLPDSGLRDNNMQWDFWTLNPESAHQVTYVMGDRGLPRTWRHMEG
                     YGSHTYMWVNPGGEKFWVKYHFKTEQGMEFFSNAEAATMAGEDADFHRRDLFEAIARG
                     DHPSWTVSVQVMPYEDAKDYRFNPFDLTKVWPHSDYPLIKVGRFTLNQNPENFFAQIE
                     QAAFSPGNLVPGIGLSPDKMLLGRAFAYHDAQRNRIGTNFHQLPVNRPRHGIDVNTYM
                     FDGQMAYEHSGNAPVYAPNSFGRGYADETGTVDTSWEVDGDMVRSAYQPHSEDDDFGQ
                     PGTLVREVFGDAERNALVEQVAGSLLGGVRSPVLERAFEYWKNIDAGIGRRIERKVKE
                     ASATEPAEGMGEG"
BASE COUNT         1203 a         2497 c         2429 g         1210 t
ORIGIN      
        1 ctacgacgcg gattttccac ccgtggcgcg cgagttcaag caacggatcg cggacgccga
       61 tgcgatcctc ttcatcacgc ccgaatacaa ccgctcgata ccgggcggac tgaagaacgc
      121 gatcgactgg gccagccggc cttggggcaa gaattcgttc gcacgcaagc cgtccgcgat
      181 catcgggaca tcccccggcg cgatcggcac cgcgattgcg caacaaagcc tccgcggcgt
      241 gttgtgcttc tgcaactcgc cgttgatgaa cgcggtggag gcgtacatcc agttcacgcc
      301 gaatctcatc gacgatgacg gggaggtcgg caacgaagcc acgaccgagt tcctgcgcaa
      361 ctacatggcc gaattcagtg cgttcataag acgcgtgaag atggcgctgt cctgacggtc
      421 agcaggaccg gctgcgcacc tcgccaacgg gaagcggcgc ccgcatcctc atggctcgtc
      481 ttcggacggg cgcgcgtggg gaagatgcca accgtggcga atcgccatgt agcggagcgc
      541 gaagcacgcc agccccccca ccacggtcgt agccacggcg tggatgtgca ggacgtggcc
      601 gccgaccacg attcccgccc cgaccagcgc ggccagcgcg tacaggtcgg agcggaggac
      661 ggtcgggacc tgcatcacca gaacatcgcg caacatgccg ccgccgatgc cggtgagcat
      721 gccgagcagg gcggccatca ccgggttcat cccgtgcacc agggctttct ccgcgcccgc
      781 cacggcgaaa aacgccaagc cgacagcgtc gaaccacagc accacgttgt tgtggcgcgt
      841 caccagtcga tcctggaaga acacgatcaa cccggccagg aacgacacgc cgaggtagtt
      901 cagacttgca atggctgccg gcgggaccgc gccgatcagc aggtcgcgcg tgatgccgcc
      961 gacgttgccg gccgcgaatg ccagcaccat cacgccgaag atgtccaggc ggtgcttcac
     1021 cgcagccacc gcgccgctga tcgcgaacac gaaggtgccg cctaggtcga gcaccgccac
     1081 caggatgtgg gtcatggttc cgcgtcccct gccgacaacg cgggactata cgcccccatt
     1141 cgggggtgcc ttcaagcaga cagccatgcg cgcgaggcga ccaccagcgc ctccaccccg
     1201 gtttccaggg tcgggtggat caccggcagg aaccgcggat tgtggttggt cgggatctcg
     1261 ttgagtcgat tcgaggcctt ggcctgcgca tacacatccg gatcggtacc gcccacgaac
     1321 cagaacaccg agggcacgcc ccactccgcc ccgaatgatc cgaaatcctc gctcgcggat
     1381 gccggcccgg tttcgtggac gcgcgcgggc gaaaaatacc cacggaaagc ctcgacgacc
     1441 cgtttactcg caccggcatc gttgctggcg agcgggtagc gatccagcgg cgtgatttcc
     1501 ggcggcttcg gtgcgcccga ggccatggct tccgcattga cgatgcgctc gatggctgca
     1561 agcacgcgct ttcgcacacc ctcgtcgaac gtacgcacgt tgagcttgat gatggcttcg
     1621 tcgggaatca cgttctcctt ggttccggcc tgcagcgcgc cgacggtgac cactgccgac
     1681 tccgccgccg ccacctcgcg cgacacgatc gtctgcagcc gcatcaccac cgccgcggcc
     1741 atcaccacag gatcgatgct cgactgcggc atcgatccat gtgccccgcg cccgaacagc
     1801 cggatctgca ggctgtcggc agcggaggtg atcgcgccgg ttcgcccgcc cagcgcgccg
     1861 gcaggcccca ccatcacgtg ctggcccagc accacgtccg gtttcgggaa acgcttgaac
     1921 aggccgtcgt cgatcatcgc cttcgcgccc gccgccgtct cttcagccgg ctggaacacc
     1981 gccatcaagg tgccgtgcca ggcatcgcgc gcctgcgcca tcagcgcggt ggcgccggcc
     2041 agccaggtga cgtgcatgtc gtggccacag gcgtgcatca ccggcacgct tttgccatcg
     2101 ggatcgcttg cggtgaccgt gctcgcataa gccacgccgg tcgcttcctc gaccggcagc
     2161 gcgtccatgt cggcgcgcag catcaccgtc gggccatcgc cattgcgcag caacccgacg
     2221 acaccggtgt tgccgatgcc ggtcgtcacc tcgaagccgg cggcccgcag acgatcagcg
     2281 gccaggcccg acgtgcgcga ttcctgcatg gacaattcgg ggtgcgagtg cacgtccttg
     2341 tacagggctt tcaggtcggg caacagccga tcgaggccgc cgagtacggg atcgggcgcg
     2401 cctgccgtcg aaacattcat ggcgactcct tcacaacgag tcattccgtg cgtacgccgc
     2461 gggcgtgcgc gtcgtgccgc attcgtggca acgagtctgc agcatgctac tccttccgtc
     2521 cggacggccc caactggcgc ggtggctcca gccggcgttc ccgcggtcac gtgtcgccgg
     2581 atggcggagc acgcaccgtc agtacatcac cgggcgcggc ttccacaatg cgggtggcgt
     2641 tgccgccgac aacgatccgg aatgccagtc cgcgcttcag ggctccgatc accgtgaggt
     2701 caatgtcgtt ggcgagtgca cgccggcgca acaggaggtc cggcggaccg tattcgatct
     2761 cggtttgcag tcgacggccg gtgccgtggg gcaggcgcgc gccgtccacg aacgactgca
     2821 tggcttcccg ctccagccgt gcgaacgcct gcccgcgatc ggcatccagc aacagcgacc
     2881 ggtggggcac gtcgagcgcg tgcatgagcg tgaagtccgc gtccgcgaac caggcgaccg
     2941 cggtctcgag tccatccagg gattcgggcg tgaaatcggt tccgaccagc accttccggt
     3001 acgccccgtg cggacgcgcc ttgacgatca gcagcggcca cggcgatcgg cgcaacaact
     3061 ggacagtggt ggacctgccg acgagatcgg caaatatcgg gccgcgggtg cccacgacga
     3121 ccaggccgca accctcgcgc tccgcgattt cgaggatcct gtcggcgggc tcgccttcgg
     3181 cgatgtggac caccaggtca tcaggcgcgt cgtgcaggtc gcgccggagc tggcgttcaa
     3241 cccgctccgc ttcgccgtcc gcagacgccc cgtcatcgcc ggagatccac cagttgtcgg
     3301 ggggtccggg ttgcagcacg tggatcacgt gcagcttcgc ctgccattgc cgcgccagca
     3361 tggccgcacg gtcgatggca cggtcggaat gggcttccag atcggtggcc agcaggatgc
     3421 gttgcggtgg ccgcgcgaaa gcgtcgcggt cgtccatgtg cttctcccgg tgccgcggtc
     3481 cattggacct ccacgggcaa gtctcaccgg ttgggaggga cccgcgcaag cggacggtct
     3541 gtccaccgcg ggtgggttcg cgttattccg gacgaacccg tgcaacgttc tagtcccttc
     3601 ggcgtacgac gcccgcctga cgggggcggg attcgcgcgt gcaaacatcc catggaatcg
     3661 aatcgcgatg cgaggggggc gccatcctgg ctcaccgtga ccactcgaat tcgcggaagc
     3721 gatccaccag tgtgtcgatc aatcggcgta ccgcgcggct gacgtagcgc gtgggcggat
     3781 acacggcatg gatggcgtcc tcggtgggcg gcgtgcgcgg cagcacgacc tgcaattgcc
     3841 ccgccgcgag cgcgggcccg gcgatgaaca gcggcaggat ggccaggccg agcccggcca
     3901 tggccgcatc gcgcatgccc tcgccgttgt tggtgtacac gcgcccgcgc acggcgatct
     3961 ggcgtggctc tccgccgcgt cgcgccggcg cgaattgcca gaacggtgtc gagcttgcgt
     4021 tgccatacgc gatgcacgga tgctgctgga tgtcctcgat ccgcttcggc agcgcgtggc
     4081 cggccgcgta ggcggggctg cagcaaagca cgcgcggact gcgcgcgatg cgccgcgcca
     4141 tcaacgagga atcggccaaa cggccgatcc gcacacccat gtcgaagcct tcgccggcaa
     4201 ggtcgacgta gcgatcgtcg aggctcaggg tgatcgcaag ccccggatgg cgtgccgcaa
     4261 acccgaacac cgagcggccg aggtatgtcg caccgaacgt caccggtgcc gtgatgcgca
     4321 ggtttcccgc gagcgcgcca tcgccgccgg cggcctcatc ggcagcctgc tggatgcctc
     4381 ccagcaggtc gagcatccgc gcatggaagc gccgacccgc atcggtgacg gcggcgcccc
     4441 gggccgtgcg atgcaacaac tgcacgtgca gcgcggcctc gaggcgcgct atccgcgcgc
     4501 tgaggctcga cttcgcgaca ccgagccggt ttgccgcggc agtgatgctg ccgtcgtcca
     4561 cgacctgcac gaacgcgcgg atatcagcga ggttgtagtc cacggacggt actcccaggg
     4621 gagtgggtgt tcggtttttc cgaacgccag gttcgccgct tcgagtctac ccaggttcgc
     4681 ggctggcgcc tatggtgggc gcataccgca acaggagatt cagtcatgta ctcgatccga
     4741 cccgccggcg aacgtggcca gtcccgcatg ggctggctcg acagccgcca cacgttttcc
     4801 ttcggcaact accacgaccc gatccacatg ggcttcggcg ccctgcgcgt catcaacgag
     4861 gatcgcgtgc agccgggcca gggcttcggc acgcattcgc accgcgacat ggaaatcctg
     4921 tcgtgggtgc tcgacggcgc actggaacac cgggacagca tgggcacgca cggcgtgatc
     4981 cgcccgggcg aattgcagcg catgagcgcg ggcaccggcg tcacgcactc ggagttcaac
     5041 gcatccgcga gcgagcccgt gcacttcctc cagatctggc tgatcccgga acgggagggg
     5101 ctggcaccct cgtacgagca acgcgcattc gcaccggccg acctcacgga ccgtttgcat
     5161 ttgatcgcgt cgcatgacgg ccgggacgat tccatcgagg tgcaccagga cgtgaacctg
     5221 tacgcgagtc ggttggcgcc ggacgcgacg gtctccttcc gcccggctgc gggacgatcg
     5281 ctgtggctgc acgtcgcgaa ggggcgtgtc caggtggacg atcgcgagct gaaggcgggc
     5341 gatgccgcat cctggacggc cgcgcccgat gctttggaca tccgggccac cgagcccagc
     5401 gaagtattgt tgttcgatct cgcgccgacg caatgagccg gtacgtcaat ccattccaca
     5461 accgcctgat acgtcgaaaa gccggcgagc tcgagtacgc cagatcaccc atcgtcctca
     5521 tccggacgac gaattggaac gactgagccc cgacgcccat caggccccaa ctggagatcc
     5581 cacatgaaaa cgctcaccaa cagtgccgac ctgctcggcc ggctgttcct ggtcctgctg
     5641 ttcgtggttg ccggttacgg caagatcggc ggctacgcgg gcacggccgc ttacatggcc
     5701 gcgaatggcg taccgggcct gctgctgccg ctcgtgatcg cgctcgaact cggcggcggg
     5761 atcctggtcc tgctcggctg gcatacacgg atcatgtcgc tgctgctggc cggattcacg
     5821 ctcctggcac tgctgctgtt ccacatgccc atgaccgcgg aaaaccagat cgtgttcctg
     5881 gctgagctgg gcgtggcggg cggcttcctc gtgcttgcag cgcatggccc gggcgcatgg
     5941 agcctggaca ggtggcttgc ccgccgcgcg cctgcgaaag cggtaactgc gtaagccatc
     6001 cggtggcgtt gcgcaggccg taacgactgc gggcccgcag cgatggctgc ggacccgcga
     6061 cttcgaagat caatggaccc gggttgcgcc gggtggcgag ccgggatcga ccagggcttg
     6121 ctcagccctc gcccatcccc tccgccggtt cagttgcact ggcttccttg accttccgtt
     6181 cgatgcgccg gccgatcccg gcgtcgatgt tcttccagta ctcgaacgcg cgttccagca
     6241 ccgggctgcg cacgccgccg agcaagctgc cggcgacctg ctcgaccagc gcgttgcgct
     6301 cggcatcacc gaacacttcg cgcaccaagg tgccgggctg gccgaagtcg tcgtcctcgg
     6361 agtgcggctg gtaggcgctg cgcaccatgt cgccgtcgac ctcccagctg gtgtccaccg
     6421 tgccggtctc gtcggcgtag ccgcggccga agctgttggg cgcgtagacc ggcgcgttgc
     6481 cgctgtgctc gtaggccatc tggccgtcga acatgtaggt gttgacgtcg atcccgtgac
     6541 gcgggcggtt caccggcagc tggtggaagt tcgtgccgat ccggttgcgc tgcgcgtcgt
     6601 ggtaggcgaa cgcgcggccc agcagcatct tgtcggggga caggccgatg cccggcacga
     6661 ggttgcccgg ggagaacgcc gcctgctcga tctgggcgaa gaagttctcc gggttctggt
     6721 tcagggtgaa ccggccgacc ttgatgagcg ggtagtcgct gtgcggccac accttggtca
     6781 ggtcgaacgg gttgaaccgg tagtccttgg cgtcctcgta gggcatgacc tggacggaga
     6841 cggtccagct cgggtggtcg cccctggcga tcgcctcgaa caggtcccgc cggtggaagt
     6901 cggcgtcctc gccggccatc gtggcggcct cggcgttgct gaagaactcc atgccctgct
     6961 cggtcttgaa gtggtacttc acccagaact tctcgccgcc ggggttcacc cacatgtagg
     7021 tgtgcgagcc gtagccctcc atgtgccgcc acgtgcgcgg caggccgcgg tcgcccatga
     7081 cgtaggtgac ctggtgggcc gactccgggt tgagcgtcca gaagtcccac tgcatgttgt
     7141 tgtcccgcag gcccgagtcg ggcaggcgct tctggctgcg gatgaagtgc gggaacttca
     7201 tcgggtcgcg caggaagaag accggcgtgt tgttgccgac gatgtcgtag ttgccttcgg
     7261 acgtgtagaa cttcatcgag aagccgcgca cgtcgcgcca ggtgtccggg ctgcccatct
     7321 caccggcgac ggtggagaa
//
LOCUS       DASSNH010000159         6102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1171341, whole genome shotgun sequence.
ACCESSION   DASSNH010000159 DASSNH010000000
VERSION     DASSNH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171341"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFK00_05950"
     CDS             complement(<1..467)
                     /locus_tag="VFK00_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592530.1"
                     /translation="MDTFAVPAEFLTQPSLLVSVDGVIQDCNTAFASSIGTTPEALRG
                     RDLSGMATDADRFLGYLRASAKDPAPPTRLTFTCEGMTAVYSARGIAQGVSGESRPVL
                     LLLNRVSPAAAEPPADVAQGRPAEASARPEGDVLDVTLASIGDGVIVTDAAGRV"
     gene            947..1978
                     /gene="ltaE"
                     /locus_tag="VFK00_05955"
     CDS             947..1978
                     /gene="ltaE"
                     /locus_tag="VFK00_05955"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HET7592531.1"
                     /translation="MIDLRSDTVTRPTPAMRKAMAAAEVGDDVYGEDPTVNELESLAA
                     NMLGTQAAVFVCSGTQSNLIALLSHCQRGDEYIVGQQAHTYKYEGGGGAVLGGIQPQP
                     LEFCADGTLDLRQVAAAIKPDDNHFARTRLLCLENTMGGKVLPLAYLQRARGFVDEHK
                     LALHLDGARAFNAAVKLGVPVTEISRHFDTVSLCLSKGLGAPVGSVLCGRRDLILRAR
                     RWRKVVGGGMRQAGVLAAAGIIALQQHVERLAEDHENARRLAEGLAGIPALGVDPSSV
                     QTNMVFCTIVGTDMDALRSHLRANGVVIGTGNPLRLVTHLDVSASDIDKVVAAFGMYF
                     DKPTHCRGD"
     gene            complement(1998..3185)
                     /locus_tag="VFK00_05960"
     CDS             complement(1998..3185)
                     /locus_tag="VFK00_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7592532.1"
                     /translation="MQHAFDYLILGAGMAGEAAAQALREADAGATIGMLGAESHPPYD
                     RPPLTKKLWKDGKEEDIWRPIDKARASVSLQRRATRIDRAAHTVHDDQGDDWQYRKLL
                     IATGGTPRQLPSGGDDFIYYRTFDDYKRLRQAVKPGARIAVVGGGFIGSELAAAMAMN
                     DCRVTMMFPEDTIGARVYPESLAEAVTAYYREKGVDVRAGTTVEGGKPNGDATELRLS
                     DGSTLVADAVVAGLGITPNVELARDAGLEVDNGIVVDDHLRSSDPDIFAAGDIASFPA
                     PSLGRRLRVEHENAAISMGTRAGKCMAGQDAPYDELPFFYSDLFDLGYEAVGVLDSRL
                     ETVEQWVTPFREGIVYYLDAGRVRGALLWNTWGQVDAARNLIAAPGPFDAENVLGRLP
                     AQA"
     gene            complement(3455..4303)
                     /gene="queF"
                     /locus_tag="VFK00_05965"
     CDS             complement(3455..4303)
                     /gene="queF"
                     /locus_tag="VFK00_05965"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03138.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET7592533.1"
                     /translation="MNRKDPTYPAAPLGRQVEYPETVDPSVLFPIARAEARKPLGVDG
                     DPLPFTGADIWNAWEFSWLDERGKPCVAVAELRVPCDSPNLVESKSVKLYLGGYAMTR
                     FDHPQDARARISDDVSGKVGAPVEVALLESGAFDRLAVANFAGELLDDQQIEVSEYGP
                     PQAAHLRLRDGARVVEEALVTRLFRSVCPVTGQPDWATVQVRYRGIPIDRAGLLRYLV
                     SYRGQADFHEACVERMFHDIQRQCRPLSLLVYARFLRRGGIDINPWRATPGFATMLPN
                     LRDARQ"
     gene            complement(4455..5864)
                     /locus_tag="VFK00_05970"
     CDS             complement(4455..5864)
                     /locus_tag="VFK00_05970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7592534.1"
                     /translation="MLHRRPFPLLIFLAALLLAVPAAWADSTLPAALQAQVDKVANDA
                     LKSSGVPSASIAVVRDGKLAYVHAYGDARLDPKTAATPDMRYSIGSISKQFTAAAILM
                     LQQQGRLGLDDKVAKYFPELTDADRITLRQLLSHTSGYQDYWPQDYLMPPMKKATTPA
                     EIMDTWAEKPLDFAPGAQWQYSNTNFVIAGAIVQKLTGKTPFQFLQQHVFAPLGITDA
                     YDTNLHALPATDARGYSRFALGPLRPSEKEGVDWMFGAGELAMTAGDLARWDIAMIDQ
                     TLLEPASWKAMQTEVLLNDGLASGYGLGVFVGSMNGHRMISHDGEVMGFTAGNYVFPD
                     DRVAIVVQTNQDAVGTFRTIGMGIAKALFDVRDPAANAALEQAKAIFADLQKGKIDRS
                     LFTDDANSYFDATGLHDYQASLAPLGKPDSFTIRSARKRGGMQMRGYTATFGDKKLAI
                     TTYLLPNGKIEQYIVAPME"
BASE COUNT         1010 a         1989 c         2076 g         1027 t
ORIGIN      
        1 acgcgacccg cggcatccgt cacgatgacg ccgtccccga tgctcgcgag ggtgacgtcg
       61 aggacgtctc cttccggccg agcggaagct tcggccggcc tgccttgcgc gacgtccgcg
      121 ggtggctcgg cagcggctgg actcactcga ttaagcagca ggagaaccgg ccgcgactcc
      181 cctgacaccc cttgggcgat gccccgggcg ctgtaaaccg ctgtcatacc ttcgcacgtg
      241 aaggtaagtc tcgtgggcgg cgccgggtcc ttcgcgctgg cccgcaaata tccgagaaag
      301 cgatcggcat ccgtggccat tccggacagg tcgcgacccc gcagggcttc cggagtcgtt
      361 ccgatgctgg atgcgaatgc ggtattgcaa tcctggatga cgccgtccac cgaaacgagc
      421 agtgacggct gcgtcaggaa ttcggctgga acggcgaagg tatccatggg ctgcgcatcc
      481 gaacgctgca aaaattcttc cttgatgctt cactccacct cgaaggccgg tgactgcgtg
      541 atgctggcgg gcggcggcag aaatgcggaa atcccagctt ccaccgagct cgaagcgcag
      601 cgtgttgctg tagcggcgac gctatccgcg ctttgattgg tttgaaagac tagcacaggt
      661 tcatcgaaag cattgcggtg gacaccgtac catggcgaag tgtcgggccg cccaggccac
      721 gcatcggtga tgctgaccac ccgaaccgtc gtgcctgcac ggaatcggta cgatcgattg
      781 tgtgccacct tcgcacgcct gctcgaatgg acggggcaga ccttgcccat tgataacgtt
      841 cgcccgcgag cacggagcat ggtccgccgg gcaatccgca gcgcacatca cacggcagcc
      901 gccactccgc taaatttggg cgaatgcctc acgcgcgaac cacgccatga tcgacctcag
      961 aagcgatacc gtcacccgac ccacgcccgc catgcgcaaa gcgatggccg ccgccgaggt
     1021 gggtgacgat gtctatggcg aagatccgac ggtcaacgag ctcgaaagcc tcgccgccaa
     1081 catgctgggg acgcaagccg cggtgttcgt ctgcagcgga acgcaaagca acctcatcgc
     1141 cctgctgagc cactgccagc gcggcgacga atacatcgtc gggcagcagg cgcacaccta
     1201 caaatacgag ggcggcggcg gagcggtgct gggtggaatc cagccgcagc cgctcgaatt
     1261 ctgcgcggac ggcaccctgg atctccggca ggtcgccgcc gcgatcaagc cggacgacaa
     1321 ccatttcgcg cgcacccggc tgctgtgcct ggagaacacg atgggcggca aggtgttgcc
     1381 gctcgcctac ttgcagcgcg cgcgtggatt cgtcgacgag cacaagctgg ctttgcacct
     1441 cgacggggcg cgtgcattca acgccgccgt caagctcggg gtaccggtca ccgagatcag
     1501 ccgccacttc gatacggtgt cgctatgcct ctcgaaaggc ctcggcgcac cggtaggctc
     1561 cgtgctgtgc gggcgtcggg acctcatcct gcgggcacgc cgctggcgca aggtggtcgg
     1621 cggcggcatg cgccaggcgg gggttctggc ggctgcggga atcatcgcgc tgcagcagca
     1681 cgtggaacgc ctggcggaag accacgagaa tgcacggcgg cttgccgaag gcctcgccgg
     1741 gattccggcg ctgggcgtgg atccgtcgtc cgttcagacc aacatggtgt tctgcaccat
     1801 cgtcggcacg gacatggatg ccttgcggtc gcacttgagg gcgaacggag tcgtgatcgg
     1861 caccggcaat ccgctgcgcc tggtcacgca cctggatgtt tcggcgagcg atatcgacaa
     1921 agtcgtggcc gcattcggca tgtatttcga caagccgacg cactgccgag gagattgacg
     1981 accgcagcgc gacgccgtca cgcctgggcc ggcaacctcc ccagcacgtt ttcggcatcg
     2041 aacgggccgg gcgcggcgat caggttgcgc gcggcgtcga cctggcccca cgtgttccac
     2101 agcagtgcgc cgcgcacgcg tccggcgtcg aggtagtaca cgatcccctc gcggaagggg
     2161 gtgacccact gttctacggt ctcgaggcgc gaatcgagga cgcccacggc ctcgtagccg
     2221 aggtcgaaca ggtccgaata gaagaacggc agttcgtcgt agggggcgtc ctgcccggcc
     2281 atgcacttgc ccgcgcgggt acccatgctg atcgcggcgt tttcatgctc gacgcgcagg
     2341 cgcctgccga gcgacggcgc gggaaacgac gcgatgtcgc cggcggcgaa gatgtcggga
     2401 tcggaactgc gcaggtggtc atcgaccacg atgccgttgt cgacctcgag ccccgcgtcg
     2461 cgcgccagtt ccacgttcgg ggtgatgccg aggcccgcga ccaccgcgtc cgccaccagc
     2521 gtactgccgt cggacaggcg cagctcggtg gcgtcgccgt tcggtttgcc gccctcgacc
     2581 gtggtgccgg cgcgcacgtc cacgcccttc tcgcggtagt acgcggtcac cgcttccgcg
     2641 agtgattcgg ggtacacccg ggcaccgatg gtgtcctcgg ggaacatcat cgtcacccgg
     2701 caatcgttca tcgccatggc ggcggcgagc tcgcttccga tgaagccgcc gccgaccacg
     2761 gcgatccgcg cgccgggctt caccgcctgg cgcagccgct tgtagtcgtc gaaggtgcgg
     2821 tagtagatga aatcgtcgcc accggacggc aattggcgcg gggtgccgcc ggtcgcgatc
     2881 agcaacttgc ggtattgcca gtcgtcgccc tggtcgtcgt gcacggtgtg cgccgcgcga
     2941 tcgatccgcg tcgcgcgacg ctgcagcgaa acgctcgcgc gcgccttgtc gatcggccgc
     3001 cagatgtctt cttccttgcc gtccttccag agcttcttgg taagcggcgg gcggtcgtag
     3061 ggcggatgcg attcggcacc cagcatgccg atggtcgcgc ccgcatcggc ttcgcgcagc
     3121 gcctgcgcgg cggcttcgcc ggccatgccg gcgccaagga tgaggtaatc gaatgcgtgt
     3181 tgcatgacgg ctcccgttcg aacgcggcgt gcaacctccg aggatactcg ggggtgccgg
     3241 ggaatcacag cggaggtgtg gaaacgccgg ggcgaggcgg gaaggagttg tcgctgccgg
     3301 cgattttcga cgacacgctt tcgccgcggg tgcgtccccc cggcgcttcg cgctcgcgct
     3361 gcgcgcgcgt tcgcttcgct ccgcccttcg caatcctaag ggggggagct tgtcttcccc
     3421 cttcgcttgc gaaggtgaat cgagggcgat gtgcttactg gcgtgcgtcg cgcaggttgg
     3481 ggagcatcgt cgcgaaaccc ggggtcgccc gccacggatt gatgtcgatt ccgccgcggc
     3541 ggaggaaccg cgcgtacacg agcagcgaga gcggccggca ctgccgctgg atgtcatgga
     3601 acatgcgctc gacgcaggct tcgtggaagt cggcctggcc gcgatacgac acgaggtagc
     3661 gcagcaaccc tgcgcggtcg atcggaatgc cgcggtagcg gacctgcacg gttgcccagt
     3721 cgggttgccc ggtgaccggg cacaccgagc ggaacagccg cgtcaccagt gcctcctcca
     3781 cgacgcgtgc gccgtcgcgg aggcgcaggt gtgccgcctg cggcggcccg tattccgaca
     3841 cctcgatctg ttggtcgtcc agcaattcgc ccgcgaaatt cgcgaccgcg aggcgatcga
     3901 acgccccgga ttcgagcagc gcgacttcga ccggcgcacc gaccttcccg gaaacgtcat
     3961 cactgattcg cgcgcgcgca tcctgcggat ggtcgaagcg ggtcatggcg tagccgccga
     4021 ggtacagctt caccgacttg gattcgacca ggttgggcga atcgcacggc acccgcagtt
     4081 cagccacggc gacgcaaggc ttgccgcgct cgtccagcca ggaaaattcc cacgcgttcc
     4141 agatgtcggc gcccgtgaag ggcagcgggt cgccgtcgac gccgagcggc ttgcgcgctt
     4201 cggcgcgggc gatcggaaac agcaccgacg gatcgacggt ttccggatat tcgacctggc
     4261 ggccgagcgg cgctgcgggg tatgtcgggt ccttgcggtt catggcggat ccatccaatg
     4321 gttcgttcga agcggctgtt gtgaaaagtc aggccatgga tggccgttcc ggacttgcac
     4381 ggacgattat ccgcgacgca tcacgaatgg cagcacaacg cggcgatcag ggacgatcgc
     4441 aaagtacgac ggggtcattc catcggcgcg acgatgtact gctcgatctt gccgttgggc
     4501 aggaggtagg tggtgatggc cagcttcttg tcgccgaagg tcgcggtgta gccgcgcatc
     4561 tgcatgccgc cacgcttgcg ggcgctgcgg atcgtgaagg aatccggctt gccgagcggc
     4621 gccaggctgg cctggtagtc gtgcaggccg gtcgcgtcga aatagctgtt ggcgtcgtcg
     4681 gtgaacaggc tgcggtcgat cttgcctttc tgcaggtccg cgaagattgc cttggcctgc
     4741 tccagcgccg cgttggccgc cggatcccgg acgtcgaaca acgccttggc gatgcccatg
     4801 ccgatggtcc ggaaggtgcc gacagcgtcc tggttggtct gcaccacgat cgcgacccgg
     4861 tcatccggga agacgtagtt gccggcggtg aagcccatca cctcgccgtc gtgcgagatc
     4921 atccggtggc cgttcatgct gccgacgaac acgccgaggc cgtagccgga ggcgaggcca
     4981 tcgttcaaca acacctcggt ctgcatcgcc ttccacgacg ccggctccag cagcgtctgg
     5041 tcgatcatgg ctatgtccca cctggcgagg tcgcccgcgg tcatcgccag ctcgccggcc
     5101 ccgaacatcc aatccacgcc ctccttctcg gaaggacgca gcggacccag cgcgaatcgc
     5161 gagtagcccc gcgcatcggt ggccggcagc gcgtgcaggt tggtgtcgta cgcatcggtg
     5221 atccccagcg gcgcgaacac gtgctgctgc aggaactgga acggcgtctt gccggtcagc
     5281 ttctggacga tcgcgcccgc gatcacgaag ttggtgttgc tgtattgcca ctgcgcgccc
     5341 ggcgcgaaat ccagcggctt ctcggcccac gtgtccatga tctccgcggg cgtggtcgcc
     5401 ttcttcatcg gcggcatcag gtaatcctgc ggccagtagt cctggtagcc ggaggtgtgc
     5461 gacaggagct gccggagcgt gatccggtcg gcgtcggtga gctcggggaa atacttcgcg
     5521 accttgtcgt ccaggcccag gcgcccctgc tgctgaagca tgaggatcgc cgcggccgtg
     5581 aactgcttgc tgatcgagcc gatgctgtag cgcatgtcgg gcgtcgcggc agtcttcggg
     5641 tccagccgcg catcgccgta ggcgtggacg taggcgagct tgccgtcgcg caccaccgcg
     5701 atggaggcgc tgggaacgcc gctgcttttc agggcgtcat tggcgacctt gtcgacctgc
     5761 gcctgcaacg ccgcgggcaa cgtcgaatcg gcccacgcgg ccgggacagc cagcagcaac
     5821 gcggcgagga aaatgagcaa cgggaaaggg cgccgatgga gcatcgttgg aattcctgca
     5881 agggcggaac ggcaacgata gcagcggtca tggctgcgcc gggacgcaaa agccatcccc
     5941 ctcatcgcgc tgcgccgggg agatggcgtc ggggaacgcg cctactcggc cggcagcggt
     6001 ggtggcagca cgtcgcgttc ggggacgcgc gcctcggcgt ggtggtcctg gcccagcagc
     6061 aggtacatcg ccggcaccac gaacagcgtg aacagggtgc cg
//
LOCUS       DASSNH010000160         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1535805, whole genome shotgun sequence.
ACCESSION   DASSNH010000160 DASSNH010000000
VERSION     DASSNH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535805"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="VFK00_05975"
     CDS             complement(<1..2216)
                     /locus_tag="VFK00_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592535.1"
                     /translation="MSASHRLTALFAALAVSSVAAASASAATPVAQSSAIPTARDNAT
                     SASTFGALHWRLIGPFRAGRTLAVTGVPGQPTHFYMGTVDGGVWETHDTGRTWQPLFD
                     DQPVQSIGAIAVAPSNPDIIYVGTGESDMRSDIVQGDGMYKSVDAGKTWTHIGLADSQ
                     QISIIRVDPHNPGRVFVAALGHPYGPNAQRGVFRSLDGGKTWKKVLGPNDDTGAIDLI
                     FKPGDANVIYASLWQTRRPPWNIYPPSNGPGSGLYKSIDGGNTWTHIEGHGFPSEKLG
                     RIGIAISAAAPDRIYAIVDAEQGGLYRSDDAGKTWKKTSGDPRIWQRGWYFGQITADP
                     KNPDRIYAMNTIELRSDDGGKTFLPVKGDPTGDDYHELWIDPNDPDRQIMGVDQGAII
                     TVNGGKTWSSWYNQPTAQMYHVSTDNRFPYRVYGAQQDSGAAAVPSRSTYYATINMMQ
                     FHEVTAGGESGMIAPDPNDPDIVYGDKVDKLDLKTKQTRSVDPTLADPDIYRSTWTLP
                     LVFNAADKKTLYFGNQRLWQTRDGGEHWTAISPDLSRPDPAVPSNLDAPTVADNLHQG
                     ARRGVVYAIGSSPLNAKLLWVGTDDGLIWRTDDAGAHWSNVTPRQLTAWSKVGNIAPS
                     HFDQDTAFAAVDRHRLDDRKPYIYRTTDGGKTWTAIVDGIPDGDFVDVVREDPVKRGL
                     LYAGTDFGIFVSFDNGDHWHSLQQNLPAVSVRDIDVKNGDLVIATHGRGFWIMDDVS"
     gene            complement(2551..>3246)
                     /locus_tag="VFK00_05980"
     CDS             complement(2551..>3246)
                     /locus_tag="VFK00_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592536.1"
                     /translation="GVEMPKALRAEERPDRSDAPKGPLALPGSYQVKVQANGHSATQT
                     VQVVADPRVNPPTDVQRAAFETAMRLRGEAAADVAMIGRSYDMMQTLDKLLDSTMDAA
                     PGSVDAKLHASAETIKQQLAAFAKDFWLPNIQYKVPEDDLHELSPWGMTFFGVYRGVS
                     RMGPNQAPNASQRAYIAEQEAGLQPKLDTFNGSLRQAVLDFNKKAKAAGVQTLDIGDP
                     VKVGDPKLLAVRS"
BASE COUNT          497 a         1032 c         1100 g          617 t
ORIGIN      
        1 gagacgtcgt ccatgatcca gaaaccgcgg ccgtgcgtcg cgatcacgag atcgccgttc
       61 ttgacgtcga tgtcgcgcac cgacaccgcc ggcaggttct gctgcagcga atgccagtga
      121 tcgccattgt cgaacgatac gaagatgccg aaatcggtgc cggcgtagag cagcccgcgc
      181 ttcaccggat cctcgcgcac cacgtcgacg aaatcgccat cgggaatgcc atcgacgatt
      241 gccgtccagg tcttgccgcc atcggtggtg cgatagatgt acggcttgcg atcatccagc
      301 cggtggcggt cgaccgcagc gaacgcggtg tcctggtcga aatgcgaggg cgcgatgttg
      361 cccaccttgg accacgcggt gagctgcctg ggcgtgacgt tgctccagtg cgcgccggca
      421 tcgtcggtgc gccagatcag gccgtcgtcg gtgccgaccc acaggagctt ggcgttcaac
      481 ggggacgagc cgatcgcgta taccacgccg cggcgcgcgc cctggtgcag gttgtcggcg
      541 acggtgggcg cgtcgagatt ggaaggcacc gcgggatcgg gacgcgacag gtcgggactg
      601 atcgccgtcc agtgttcgcc gccgtcgcgg gtctgccaca accgctggtt gccgaaatac
      661 aacgtttttt tgtcggccgc attgaagacc agcgggagcg tccaggtgct gcggtagatg
      721 tcggggtcgg ccagcgtggg atcgacgctg cgcgtctgct tggtcttgag atccagcttg
      781 tcgaccttgt cgccgtacac gatgtcaggg tcattcggat cgggcgcaat catgccgctt
      841 tcgccgcccg cggtgacctc gtggaactgc atcatgttga tcgtcgcgta gtaggtcgag
      901 cggctgggca cggcggccgc gccggaatcc tgctgcgcgc cgtacacgcg atacgggaaa
      961 cggttgtcgg tgctgacgtg gtacatctgc gcggtcggct ggttgtacca actcgaccag
     1021 gtcttgccgc cattcacggt gatgatcgcg ccctggtcga cgcccatgat ctggcggtcc
     1081 gggtcgttcg ggtcgatcca cagctcgtgg taatcgtcgc cggtcgggtc gcccttcacc
     1141 ggcaggaagg tcttgccgcc atcgtccgaa cgcagctcga tggtgttcat cgcgtagatg
     1201 cgatcggggt tcttcggatc ggccgtgatc tgcccgaaat accagccgcg ctgccagatg
     1261 cgcggatcgc cactggtctt cttccaggtc ttgccggcat cgtcggagcg gtacaggccg
     1321 ccctgctccg catcgacgat cgcgtagatg cgatcgggcg cggcggcgga aatcgcgatg
     1381 ccgatgcgtc cgagtttttc cgacgggaaa ccgtggcctt cgatgtgcgt ccaggtgttg
     1441 ccgccgtcga tggatttgta gaggcccgag cccggcccgt tggagggcgg gtagatgttc
     1501 cacggcggac gccgcgtctg ccacagcgac gcgtagatca cgttggcatc gccgggcttg
     1561 aagatgaggt cgatcgcgcc ggtgtcgtcg ttcggaccga gcaccttctt ccaggtcttg
     1621 ccgccgtcga gcgaacggaa cacgccgcgc tgggcgttcg gcccgtaggg atgccccagc
     1681 gccgcgacga atacgcggcc cgggttgtgc ggatcgacgc ggatgatcga aatctgctgc
     1741 gaatcggcga ggccgatgtg cgtccaggtc ttgccggcat cgacggactt gtacatgccg
     1801 tcgccctgca cgatgtccga acgcatgtcc gattcgccgg tgccgacgta gatgatgtcc
     1861 gggttcgacg gcgccaccgc gatggcgccg atcgactgca ccggctgatc gtcgaacagc
     1921 ggctgccagg tgcggccggt gtcgtgggtt tcccacacgc cgccgtcgac ggtgcccatg
     1981 tagaaatgcg tcggttggcc gggaacgccg gtgacagcca gtgtgcgacc ggcgcggaat
     2041 ggaccgatca ggcgccagtg gagcgcgccg aatgttgatg cgctcgtcgc gttgtcccgc
     2101 gcagtgggaa tcgcgctgct ttgcgcgacg ggtgtcgcgg ccgatgcgga cgctgcagca
     2161 accgaggaca cggcgagggc ggcgaacagc gcggtcaagc gatgggacgc agacatgaac
     2221 gactcctcac ggcgcaggca atccttgcat catagcgagg ccgcgcctgc gttcatgggt
     2281 aagagggcat acccggtcgc gccatctttg cttgtgcgcc ttctcccttc gggagaaggg
     2341 gcgtgaaacg ccgcctcttc ttttgcgcga agcgccggat gagggtccgg gacttgcgcg
     2401 taatgggtac ccccgtagcg ccacgcgagc gcgtaccctc acccgcccct gcggggcacc
     2461 ctctcccgga gggagaggga ttcaaagcgc cagcccgccg cagccaacca accaacgacg
     2521 caattctggt gatccgcgct gcccaccgtc tcaactcctt accgccaaca acttcggatc
     2581 gccgaccttc accggatcgc cgatgtccag cgtctgcacg ccggccgcct tcgccttctt
     2641 gttgaagtcg agcacggctt gtcgcagcga cccgttgaag gtgtcgagct tcggttgcag
     2701 ccccgcttcc tgctcggcga tgtatgcgcg ctggctggcg ttcggtgcct ggtttggccc
     2761 catgcgcgac acgccgcggt acacgccgaa gaaggtcatg ccccagggcg agagctcgtg
     2821 caggtcgtct tccggcacct tgtactggat gttcggcagc cagaagtcct tcgcgaacgc
     2881 ggcgagctgt tgcttgatgg tttcggcgct ggcgtggagc ttggcgtcga cgctgcccgg
     2941 cgccgcatcc atggtcgaat ccagcagctt gtcgagcgtt tgcatcatgt cgtagctgcg
     3001 gccgatcatc gcgacgtcgg ccgcggcttc gccacgcagt cgcatcgcgg tttcgaacgc
     3061 ggcgcgctgc acgtccgtgg gcggattgac gcgcggatcg gcgaccacct gcacggtttg
     3121 cgtcgcgctg tggccgttgg cctgcacttt tacctgatac gaaccgggta gtgcgagcgg
     3181 gcccttgggc gcgtcgctcc tgtccggccg ctcttccgca cgcagcgcct tcggcatttc
     3241 gacgcc
//
LOCUS       DASSNH010000161         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1396614, whole genome shotgun sequence.
ACCESSION   DASSNH010000161 DASSNH010000000
VERSION     DASSNH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396614"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..1225)
                     /locus_tag="VFK00_05985"
     CDS             complement(428..1225)
                     /locus_tag="VFK00_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HET7592537.1"
                     /translation="MTHPCPLIGDARCILIEGDDARRFAQAQFSGNVDALAPGYWQWN
                     AWLTPQGRVSALMHLVDAGDGRLLAVLRGGDADASRAGLARYLLRQRAALRVEAFACR
                     SGDPATMGTVAIDAGEIVLGYGPRSLRLGTTANEPPDADARTTWRLEDIRQGWPSLPG
                     GELLFLPPALGLEHLQAVSFDKGCYPGQEIAARLHYRGGHKHRLYHLQAPATPPAYAL
                     ALPEDTTCLDAVTHGGITDALVVGPLDLPQETSAATRPFRVISSFDS"
     gene            1328..1492
                     /locus_tag="VFK00_05990"
     CDS             1328..1492
                     /locus_tag="VFK00_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HET7592538.1"
                     /translation="MDKDSSNTVKPGDSEPAPDSPPASAPRERPTPEGDGLDPTRYGD
                     WEKNGRCIDF"
     gene            1724..2143
                     /gene="sdhC"
                     /locus_tag="VFK00_05995"
     CDS             1724..2143
                     /gene="sdhC"
                     /locus_tag="VFK00_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HET7592539.1"
                     /translation="MPTTRPLSPHLGVYRWRVNMLQSTLHRLTGLFLCLGALLVTWAL
                     IAAATGPSAWGVFAGFWNSWIGEVLLFLWLWSLWFHLCNGIQHLARDAGLNYGPANRD
                     RTHNPSYWSTGWIILVVSVALTVLVWVLLSLPIGGGA"
     gene            2140..2550
                     /gene="sdhD"
                     /locus_tag="VFK00_06000"
     CDS             2140..2550
                     /gene="sdhD"
                     /locus_tag="VFK00_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HET7592540.1"
                     /translation="MSGYPGQEGLRTPLKVARGLGSSRSGVHHWWVQRVTAVALVPLS
                     IWFLFVLGGMLHAASFADVVATIAQPVHAIFLIVFTICTFWHGALGLQVVIEDYVHTR
                     WLEVTLEIALRFGALLGALACVMAIAALWLNGTP"
     gene            2725..>2870
                     /locus_tag="VFK00_06005"
     CDS             2725..>2870
                     /locus_tag="VFK00_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7592541.1"
                     /translation="MPNESYKIETHDYDVVVVGAGGAGLRATLGLAAKGLSAACVTKV
                     FPTRS"
BASE COUNT          472 a          905 c          999 g          494 t
ORIGIN      
        1 gctgcgccgc aacggatgga gcgttggctg gaacctggaa gcgatcgccg gcgaggggcc
       61 ggggctcggg ggttcgcaat gcgccaacga cgttcggcaa accggcgaat tcttcgccgt
      121 ctcacgtgat gcggggtccg cagatcgcgt gctgcccccg ggctgaagca ggcccgggct
      181 ctggatcggc gggaagccta agggcgggcc cgggtcggca caagtgccaa gcgcttcaca
      241 aaaccgttga cgtttagact ggattgggga atgtagttga gaattgacat catatgacgc
      301 cgacaaccaa ctgaatattt atcactcttg cctggccctg gtccccgcgg ggtcattcgc
      361 aaggacgtgg gtgtgccggg gcgggcgggg agagggggcc tccggaccct ggatcatgga
      421 tcgtggatca ggaatcaaag ctggatatca cccgaaaagg tcgggtcgcc gccgaagttt
      481 cctggggcaa atccagcggg ccaacgacca gcgcgtcggt gattccgcca tgggtgacgg
      541 cgtccaggca ggtggtgtcc tccgggagcg ccagggcata ggccggcggc gttgcgggag
      601 cctggaggtg gtacaggcgg tgcttgtggc cgccgcggta gtggaggcgc gccgcgattt
      661 cctggcctgg atagcagccc ttgtcgaacg acaccgcctg caggtgctcg agccccagcg
      721 ccggcggaag gaacaagagt tcgcccccag ggaggctggg ccagccctgg cggatgtctt
      781 cgaggcgcca ggtagttcgg gcatccgcgt cgggcggctc gttggctgtg gtccccaggc
      841 gcaggctgcg ggggccatag ccgagcacga tctctcccgc gtcgatggca accgtcccca
      901 tcgtggcggg atcgcctgag cggcacgcga acgcctccac gcggagcgcc gcgcgttgcc
      961 gcaacaggta gcgcgccagc cccgcacggc tggcatcggc atcgcccccg cgcaacactg
     1021 caagcaggcg cccgtcgcct gcatcgacga ggtgcatcag cgcagacacg cggccttgcg
     1081 gggtgagcca cgcgttccac tgccagtacc ccggcgcaag cgcgtcgacg ttgccggaga
     1141 actgagcctg ggcaaagcgg cgcgcgtcgt cgccttcgat caagatgcaa cgcgcgtcgc
     1201 cgatcaatgg acaggggtgg gtcatcgcaa actcgttcga agcatcgcgt taccgtagca
     1261 gcgtcgccga tgcgcgggtg agggcgggat gcgacaatag gcactgcact tcgaaccatg
     1321 caaagcgatg gacaaggatt cctccaacac agtgaagccg ggcgattcgg aacccgcacc
     1381 tgattcgccg ccggcatccg cgccgcgcga acggccgacg ccggaaggcg acgggctcga
     1441 tcccacgcgc tacggcgatt gggagaagaa cggccgctgc atagacttct agcgagatcc
     1501 tccatgatcg ccgcgtcgtc tgacgcgcgg tgagcggctg cgtttcgagt ctgctacaca
     1561 ccagtgccgg aatcctggcc gcactctcgg caacacagcc ccttcgcctg cattgtggat
     1621 ggtcttcgcg tacaggcgca ttcatgcgac aatggaaagc gatcatcggc gtcgaagccg
     1681 acgaggggat gatgttgctc gcgcagtgat caggactgag gacatgccca ctacccgacc
     1741 gttgtcaccg cacctcggcg tttaccgctg gcgcgtcaac atgctgcagt cgacgttgca
     1801 ccggctgacg ggactgttcc tgtgcctggg tgcactgctg gtgacgtggg cgctgatcgc
     1861 cgccgccacc ggcccttcgg cgtggggagt gttcgccgga ttctggaata gctggatcgg
     1921 cgaggtcctg ttgttcctgt ggctgtggtc gttgtggttc cacctgtgca acggcatcca
     1981 gcacctggcc cgcgacgccg ggttgaacta cggacctgcc aatcgcgacc gtacgcacaa
     2041 cccgtcctac tggtccacgg gctggatcat ccttgtcgtc agcgtcgcac tcaccgtgct
     2101 ggtgtgggtg ctgctctcgc tgccgatcgg agggggcgca tgagcggtta tccgggacag
     2161 gagggcctgc gcacgccgct caaggtggcg cgcggcctgg gttcctccag gagcggcgtg
     2221 caccactggt gggtgcagcg ggtaaccgcg gtcgcgctgg tgccgctgtc gatctggttc
     2281 ctgttcgtgc tcggggggat gctgcacgcc gcgagcttcg cggacgtggt cgcgaccatc
     2341 gcccagccgg tccacgcgat cttcctgatc gtgttcacga tatgcacgtt ctggcacggc
     2401 gcactgggcc tgcaggtggt catcgaggat tacgtgcaca cccgctggct ggaagtgacg
     2461 ctcgagatcg ccttgcgctt cggcgcgctg ctgggcgcgc tggcgtgcgt gatggcgatc
     2521 gcagcgctgt ggctcaacgg tacaccttga cggtaccggg caacgcctga tcgtcgggtt
     2581 cccgcggtgc agggacgtgc cgcttcggat cgaatgccac gggcgttcga tccggacgcc
     2641 ggatccggac tgccggaccg gcgcgcactg gcgagtcccg gatgactgcc agcgcatcgg
     2701 aaattgctcg cacaagggat gcacatgccc aacgagagtt acaagatcga aacccatgat
     2761 tacgacgtgg tcgtggtcgg cgccggcggc gcgggcctgc gcgccaccct gggcctcgcc
     2821 gcgaagggac tctccgccgc ctgcgtcacc aaggtgtttc cgacccgctc
//
LOCUS       DASSNH010000162         7001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1859669, whole genome shotgun sequence.
ACCESSION   DASSNH010000162 DASSNH010000000
VERSION     DASSNH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859669"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..373)
                     /locus_tag="VFK00_06010"
     CDS             complement(194..373)
                     /locus_tag="VFK00_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592542.1"
                     /translation="MRYHLAEAIRINGLRGDRRRAEFAKLPLGTRTTIAVLAKHLQGR
                     THEAHCDARASERAR"
     gene            complement(475..894)
                     /locus_tag="VFK00_06015"
     CDS             complement(475..894)
                     /locus_tag="VFK00_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592543.1"
                     /translation="MTDTPIFDEYVRNRLNAWGRVFPMWDRYGPELGSGTHMLVRLIE
                     HKGEIPPPNVGFKPLTVPPDEMEIEDIVREIHAELPDVAHVLRAVYGGWGRIGVERVR
                     LAERLAGKPIKRRQLYALHDVGFHRVAGALMMSARAA"
     gene            complement(891..1277)
                     /locus_tag="VFK00_06020"
     CDS             complement(891..1277)
                     /locus_tag="VFK00_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HET7592544.1"
                     /translation="MIRLRLPWPPSVNRYWRHPTVGKLAGRHLISEEGRTYRADVTAA
                     VYAQLRCQPKLDGRLAVHIDVHAPDKRARDLDNLPKGIQDSLGHAGVWRDDSQIDDLR
                     VVRRETVRGGLVVVLIRQLDSLEDAA"
     gene            complement(1274..2677)
                     /locus_tag="VFK00_06025"
     CDS             complement(1274..2677)
                     /locus_tag="VFK00_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7592545.1"
                     /translation="MIETEKTAQPCGLANVSCSEFTPQTAQQASALDQLMTFATGKHG
                     ARAMTLAGYAGTGKTTLVGELVRRLGQEMAIAVAAPTHKAVSVLREKVGEAAPAEFAT
                     LHSLLGLRLKPQHDGTYRCLPEGVPTIGEFALVIIDEASMVHRELFSLVMSPAYQGTR
                     LLFVGDPAQLPPVEDAGETSMAFSRIQHQVMLTDVCRQAAEHPAIQLSMRIRAAIEQG
                     VRLDAGRVASLCASRDADVLFGTGGGVTAFNWAFYDLKAGHDTRILAWRNRTVRAYNR
                     DLHVALHGDATPFAVGETLMLNDSHGGRLDDGGDPMRALKVTLHNSEETVVHAITRAL
                     HPRHEDVSAWRIVVQRDNGQRAVLWIADNEDVRQAQISAMFRRASDIKRQMTAGFNAP
                     LDAERVELIRKAWALREAFADARHVYAMTVHKSQGSTFDTAIVDVADLETCRGDEYNQ
                     LLYVAATRPRYHLAFIA"
     gene            complement(2947..5190)
                     /locus_tag="VFK00_06030"
     CDS             complement(2947..5190)
                     /locus_tag="VFK00_06030"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HET7592546.1"
                     /translation="MAQSAYPATPLEHALAYAALGFKVFPLHAMASGRCTCGDADCRS
                     PAKHPRTLRGAHDAACDEAAIRAWWTQWPDANIGMTLEGLAVVDVDPRNGGDDALTAL
                     QAKHGRLPETATALTGGGGQHYLYRVAESRRLPGKLGDGIDLKHGPGAYIVVAPSIHA
                     SGVAYCWEASADLLDGFPLTNLPDWIGEQASTTSPPPRVVGLLDAKRKRDLRGALAYI
                     DADDRDNWITVGMALHSTEAADAFAVWTEWSQLSPKYNPGDQRRVWNSFHPDGKTHVE
                     TIFRMAERAGWVNPDSRESTRYNDALEAAIAAANARTHIEAVEEKPQVEIGPFPAPAL
                     NELSAWIDARFALTHPAVTRQITLALAAVAASRNYVGEGGTPCHLCLGIVADSAALTA
                     YARDAVSLVLDACGLRRMARGTRANVPSNVYTMLWRSPAAIHVVNDFGHLAAYAKRQP
                     SGVLDQVFSVMAEAHTAVALYLDSAAEIGLKPGASDDQLVIHAPALTTLLLSTTAQMG
                     ALLQKDEISRGLLAWQLPVIARTGSTTEKVPSSAPLPQSLRDHLRAVRRLPAGTGELS
                     LQDIFGTQPGLLPRVTRIRWERPFDEYADAIRAISKDPAHRVVLMTAVDAARRIANAL
                     APWRDPAAPVATREVMDWAAAYVIRLMGDWLEEYDTLGTDDGIVGIGQKVLAAVRDRK
                     GAGIPRGRLHAYCRAYKQLGKDRRAQVLDALLEDGDLVEFAPRGQRAKVLVHSKFAKT
                     TKAGDAA"
     gene            complement(5196..5390)
                     /locus_tag="VFK00_06035"
     CDS             complement(5196..5390)
                     /locus_tag="VFK00_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592547.1"
                     /translation="MNVCTPMPDGSLHCLTPHEAQVLRGYLLDRMVHVDERDQHVLLR
                     EVERLKAVADPMAAYEDWGL"
     gene            complement(5387..5788)
                     /locus_tag="VFK00_06040"
     CDS             complement(5387..5788)
                     /locus_tag="VFK00_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592548.1"
                     /translation="MNAELTYKRDLKPVPEEDWRKLTDEGKGVRYACEHSGMHDKSIA
                     LESNIDNALLSRAKAGEARLPFDALNALMDSTGCEAPLYAYLLRRGYDPRSLRKLETE
                     TERALRMKTEELEAEKLKVRVLTEALRGTAA"
     gene            complement(5905..6135)
                     /locus_tag="VFK00_06045"
     CDS             complement(5905..6135)
                     /locus_tag="VFK00_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7592549.1"
                     /translation="MNTEPIKRAAEKLGSHRGLATAAGVTAGMVSQWVNGVRPVAAHH
                     CIRIEQATQGAVTRYELRPDVFGEAPPAAKAA"
     gene            6165..6695
                     /locus_tag="VFK00_06050"
     CDS             6165..6695
                     /locus_tag="VFK00_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592550.1"
                     /translation="MQQRCWQTRNAAVNSIANMKKLTPTPESAALDNAMADRRVHNAT
                     LARHVKVSDGLVSQWRTGRRPVPAHYAPKVAGFLDMQDPALISAQYAQVRAAQTGNGV
                     AIPGVDAFPDDLALRRLENAIDELRHAVAAMITASIIHRPAEARDVAKAIRRHAAPRS
                     LRSGLLAELLQALEKA"
BASE COUNT         1148 a         2335 c         2193 g         1325 t
ORIGIN      
        1 atgccggcaa cccaggcaat tgcccgccac gcaccacggg cggaaatgac cgccgcgtgt
       61 acttcgtcca tcgttgactc gatgcgatcg agtcttgaat cgatccgccc ttcgagggct
      121 gacatgcggc tttccatcgc cgacatccgc gcatccagat tgccgagcgt gcgctccacg
      181 tgcagcccct cactcaccgc gcccgctccg atgcgcgcgc gtcgcaatgt gcctcatggg
      241 tacgcccctg taggtgtttc gccagcaccg cgatcgtggt cctggtcccg agcggtaatt
      301 tcgcgaattc cgcgcgccgg cggtcaccgc gcagtccgtt gatccggatt gcttcggcaa
      361 ggtggtagcg catggattcc gaaaacgaaa aaaccccgct cgaggcgggg ttttgaaatg
      421 ttctctgaca gcttcgtcag cttgccgcga atcctacgct ttttgtgcgc acagtcaagc
      481 ggcgcgagcg gacatcatca gcgcacccgc tacccgatga aacccgacgt catgcagcgc
      541 atacagctgg cggcgcttga tcggtttacc agcaagcctt tcggcgaggc ggacgcgctc
      601 gaccccgatc cggccccacc ctccgtatac cgcacgcagg acgtgcgcga cgtcgggaag
      661 ttctgcatgg atctcacgca cgatatcctc gatctccatc tcatccggcg gaacggtgag
      721 gggcttgaac ccgacattcg gtggtgggat ctcgcccttg tgttcgatca ggcgcacgag
      781 catatgggta ccgctgccga gttccgggcc atagcggtcc cacatcggga aaacgcggcc
      841 ccacgcgttg aggcggttgc gcacgtactc atcgaagatc ggcgtatcgg tcaagccgcg
      901 tcctccagtg agtcgagctg ccggatcagc actaccacga ggccgccgcg taccgtctcg
      961 cggcgcacca cgcgcaggtc atcgatctgg ctgtcatcgc gccagacgcc ggcgtgaccc
     1021 agcgagtcct ggatgccctt tggcaagttg tcgaggtcgc gtgcgcgctt gtcgggcgca
     1081 tggacatcga tgtgcacggc cagccggccg tccagttttg gctggcagcg cagctgggcg
     1141 tacaccgccg cggtcacgtc ggcgcggtag gtgcgaccct cctccgatat caggtgtcgt
     1201 ccggcgagtt tcccgaccgt cggatggcgc cagtaccggt tcaccgacgg cggccatggc
     1261 aggcgcaggc ggatcatgcg atgaacgcca ggtggtagcg cggtcgcgtc gccgcgacgt
     1321 acagcagctg gttgtattca tcgccgcggc aggtttcgag atcggctaca tcgacgatcg
     1381 cggtgtcgaa agtggacccc tgcgacttgt gcaccgtcat ggcatacacg tggcgcgcgt
     1441 ccgcgaaggc ctcgcgcaag gcccaagcct tcctgatcag ctcgacccgt tcggcgtcca
     1501 gcggtgcgtt aaatcctgcc gtcatttgcc gcttgatgtc gctggcccgc cggaacatcg
     1561 cgctgatctg cgcctggcgc acgtcctcgt tgtccgcgat ccatagcacc gcgcgctggc
     1621 cgttgtcgcg ctgcacgacg atgcgccatg ccgaaacatc ctcgtggcgc gggtgcagcg
     1681 cgcgcgtgat cgcgtgcacg acggtttcct cgctgttgtg cagcgtcacc ttcagcgcgc
     1741 gcatggggtc gccgccgtcg tcgaggcgcc cgccatggct gtcgttcagc atcagggttt
     1801 cgccgaccgc gaacggcgtg gcatcgccat gcagggccac gtgcagatcg cggttgtagg
     1861 cgcgcacggt gcgattgcgc caggcgagga tgcgggtgtc gtgccctgcc ttgaggtcgt
     1921 agaacgccca gttgaatgcg gtcacgccgc caccggtacc gaacagcacg tcggcgtccc
     1981 tcgacgcgca caacgaggcc acgcgtccgg catcgagcct cacgccctgc tcgatcgcag
     2041 cgcggatccg catcgagagc tggatcgccg gatgttccgc ggcctgcctg caaacgtccg
     2101 tgagcatcac ctgatgctgg atgcggctga acgccatcga ggtctcgccg gcatcctcta
     2161 ccggcggcag ctgcgctggg tcgccgacga acagcaggcg cgtgccctgg tatgccgggc
     2221 tcatcaccag ggagaacagt tcacggtgca ccatcgaggc ctcgtcgatg atcacgagtg
     2281 caaactcccc gatcgtcgga acgccctcgg gcaggcaccg gtacgtgccg tcatgctgtg
     2341 gcttcaagcg caatccgagc aggctgtgca gcgtcgcgaa ttccgccggc gcagcctcgc
     2401 cgaccttttc gcgcagcacg ctcacggcct tgtgggttgg agcggcgacc gcgatcgcca
     2461 tttcctgccc aagcctgcgc accagttcac cgaccagcgt ggtcttgccg gtaccggcgt
     2521 acccggcgag cgtcatcgcg cgagcgccgt gcttgccggt cgcgaatgtc atcagctgat
     2581 ccagcgcgga cgcctgttgc gcggtctgcg gggtgaattc agaacaggag acattcgcca
     2641 acccgcatgg ttgtgccgtt ttttctgttt cgatcatcga actggactcg taaccgtttg
     2701 atttatttca aggagacatt tgtctcctga atttgtctcc tgaaaaccgc acctcaaacc
     2761 cgcgccgttg tgcgattcgt accctttcag gagacaggag acctatagga gatttttaaa
     2821 aattccccta taaggccgtc tcctgactcc tgtttgatcc aaatgcctga acctgaacga
     2881 aaatttcagg agacaaccgg gagacaaagg cgtctccttt tgtctcctgt ttgaggcttt
     2941 ccgggttcat gccgcgtctc ctgccttcgt ggtctttgcg aacttcgaat gcaccagcac
     3001 tttcgcccgc tggccccgtg gtgcgaactc gaccaggtcg ccgtcctcga gcagcgcgtc
     3061 gagcacctgc gcgcgccggt ccttgcctag ctgcttgtag gcgcggcagt aggcgtgcag
     3121 acgcccgcgc ggaatgcctg cgcctttgcg gtcgcgcacc gcggccagga ccttttgccc
     3181 gatcccgaca atgccgtcgt cggtgccgag cgtgtcgtac tcctcgagcc agtcgcccat
     3241 caacctgatc acgtaggccg cggcccagtc cattacctcg cgcgtggcca ccggcgcggc
     3301 cgggtcgcgc catggcgcca gggcgttcgc gatccggcgc gcggcgtcca ctgcggtcat
     3361 cagtaccacg cggtgcgcgg gatccttgct gatcgcgcgg atcgcatcgg cgtattcgtc
     3421 gaacggccgc tcccagcgga tccgcgtcac ccgcggcagc agcccgggct gcgtcccgaa
     3481 tatgtcctgc agtgacagtt cgcccgtgcc cgccggcaac cgccggaccg cgcgcaagtg
     3541 atcgcgcagg gactgcggca gcggcgcgga gctcggaacc ttctcggtcg tcgagccggt
     3601 gcgcgcgatc accggcaatt gccatgccag cagcccgcgg ctgatctcgt ccttctgcag
     3661 cagcgcaccc atctgggccg tcgtggacag cagcagcgtg gtcagtgccg gcgcatggat
     3721 caccagctgg tcatccgatg cacccggctt caggccgatc tcggccgctg aatctaggta
     3781 cagcgcgacc gcggtatgcg cctcggccat cacgctgaac acctggtcca gcaccccgct
     3841 cggctgacgc ttggcgtacg ccgcgaggtg cccgaaatca ttcactacgt ggatcgccgc
     3901 cggcgagcgc cagagcatgg tgtagacgtt gctgggcacg ttggcacgtg tgccgcgcgc
     3961 catcctgcgc agtccgcagg catccagcac cagcgatacc gcgtcgcgcg cgtaggcggt
     4021 gagcgccgcg gagtccgcga cgatgccgag gcacaagtgg catggcgtgc cgccctcgcc
     4081 aacgtaattg cggctcgcgg caaccgcggc cagggccagc gtgatctggc gcgtcacggc
     4141 cggatgggtc agcgcgaaac gcgcatcgat ccacgcagac agttcattca gcgccggagc
     4201 cggaaacggc ccgatctcga cctgaggctt ttcctcgacc gcctcgatat gggttcgtgc
     4261 gttcgccgcg gcgatcgcgg cctcgagcgc atcgttgtaa cgggtcgact cgcgcgagtc
     4321 cgggttcacc cagccagcgc gctcggccat tcggaagatt gtctcgacgt gggtcttgcc
     4381 gtctggatgg aaactgttcc agacccgccg ctggtcaccg gggttgtact tcggcgagag
     4441 ctgcgaccat tcggtccaga ccgcgaacgc gtcggcggct tcggtggagt gcagcgccat
     4501 gccgacggtg atccagttgt cgcggtcgtc ggcgtcgatg tacgccaggg ccccacgcag
     4561 gtcccgcttg cgcttggcgt ccagcagtcc gaccacgcga ggtggtgggc ttgtggtcga
     4621 cgcctgttcg ccaatccagt ccggaagatt cgtcagtgga aaaccgtcga gcaaatccgc
     4681 cgaggcctcc cagcaatacg ccacacccga cgcatggatc gatggcgcga ccacgatgta
     4741 cgcgccgggc ccgtgtttga gatcgatgcc gtcgccgagc tttccgggca accgccggct
     4801 ctcggccaca cggtacaggt agtgctgtcc gccgccgccg gtaagtgctg tcgcggtctc
     4861 cggcagtcgg ccgtgcttgg cctgcagcgc cgtcagcgcg tcatcgccgc cgtttcgtgg
     4921 atccacgtcc accacggcga ggccttcgag cgtcatgccg atgttcgcgt cgggccactg
     4981 cgtccaccag gcgcggatcg cggcctcatc gcaggccgca tcgtgcgcgc cacggagcgt
     5041 acgcggatgc ttggccggtg accggcaatc cgcgtcaccg caagtgcagc gcccgcttgc
     5101 cattgcgtgc aacggaaaca ccttgaagcc cagtgccgcg tacgccagcg cgtgttccag
     5161 cggtgtcgcg gggtacgcgc tttgcgccac ggcgttcaaa gaccccagtc ctcgtaggcc
     5221 gccattggat cggccaccgc cttgagccgc tcgacctcgc gcagcagcac atgctgatcg
     5281 cgttcgtcga cgtggaccat gcgatcgagc aggtaacctc gtagtacctg ggcctcatgc
     5341 ggggtcaggc aatgcaggct gccgtccggc atgggggtgc atacgttcat gcggctgttc
     5401 cacgcagcgc ttccgtgagc acgcgcacct tgagcttttc ggcctcgagc tcttcggtct
     5461 tcatgcgcaa cgcccgttca gtttccgttt cgagcttgcg caggctgcgc gggtcgtagc
     5521 cgcggcgcag caggtacgcg tacagcggcg cctcgcagcc cgtggaatcc atcaaggcat
     5581 tcagagcgtc gaatggaagg cgcgcctcgc ctgctttcgc gcgtgacagc aacgcattgt
     5641 cgatattcga ttcgagcgcg atcgacttgt cgtgcatgcc gctgtgttcg caggcgtagc
     5701 ggaccccctt cccttcatcc gtgagcttgc gccaatcctc ttccgggacg ggtttcaggt
     5761 cgcgtttgta ggtcagttcg gcgttcatca acggaattga ctatcgttgt aggtcgccgt
     5821 cgtggcgaaa tgaaaccgcg caatcccgcg cggctgcgag gtttcagcga tgcaacagga
     5881 caacgccgat gttgatggcc gccatcacgc ggccttcgcc gccggcggcg cttccccgaa
     5941 gacgtccggg cgcagctcgt aacgcgtcac cgcgccctgc gtggcttgtt cgatccggat
     6001 gcagtgatgg gcagccacgg ggcgcacgcc gttcacccac tggctaacca tgccggcggt
     6061 gacgccggct gccgtggcca ggcctcgatg gctaccgagc ttttcggcag cgcgcttgat
     6121 cggttcggta ttcacgccct cacgatagca tcgctgcttt tctgatgcaa cagcgttgct
     6181 ggcagacaag gaacgccgca gtgaatagca ttgctaacat gaagaagctg acacctactc
     6241 ccgaatccgc ggcgctcgac aacgccatgg cggaccggcg cgtacacaac gcgacgctgg
     6301 cccgtcatgt caaggtcagc gacgggctgg tttcgcagtg gcgaactgga aggcgcccgg
     6361 tgccggcgca ttacgcgccg aaggtcgccg gcttcctcga catgcaggac cctgcgctaa
     6421 tcagtgcgca gtatgcgcag gtgcgcgcgg cgcaaactgg caatggagtg gccattccgg
     6481 gagtcgacgc atttcctgat gacctggcgc tgcgtcgcct cgagaacgcc atcgacgaac
     6541 tgcggcatgc cgtggccgca atgatcaccg catccatcat ccatcgacca gccgaagcgc
     6601 gggatgtcgc gaaggcgatt cgtcggcacg cggcgccgcg gtcactgcga tcgggtcttc
     6661 ttgccgaact gctgcaagcc ttagaaaagg cttgaccggc agcggtgccg ggcttcgctc
     6721 ggcctcgcgc aaggcgcgta ttccttcgac gagcgcaccc atgatctcgt caatcctgtc
     6781 atccatcagt cttcccctgt tggtgctcga gccccttact caagcggctc cagtgtgcac
     6841 cgggtgagac cggatcgatc gcacgaacat gacgaaatgt gaacacgtga ctgaaatcac
     6901 gagaccctag gccgtgtgcg actttccggc atttgaaact tttttcagcg atgctattgc
     6961 attcttaaat tagctttgct atcgttgcac gcgtcgcctc a
//
LOCUS       DASSNH010000163         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1773870, whole genome shotgun sequence.
ACCESSION   DASSNH010000163 DASSNH010000000
VERSION     DASSNH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773870"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFK00_06055"
     CDS             <1..769
                     /locus_tag="VFK00_06055"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7592551.1"
                     /translation="KGGQPGGQIAFSPDGKYLFLTVGDRQRMTPAQDINQPEGKILRL
                     TLDGKPAPGNPWAGKVGARTIPLIDPPADTEAAKTAPVVSTYTFPGPNLTPAETWATG
                     FRTPYGLAFAPDGRLWELEHGPRGGDELNLIEPGKNYGWPLVSYGVNYNGVPIPNPDT
                     RPDLVKPVIYWAPVIAPGNLMFYKGNVFPWNGSALISALAAEAIVRVTFDGKGGATAV
                     QRWAIGIRVRDIEEAPDGTLWMLEDAKGGGLYHLTPR"
     gene            complement(921..1361)
                     /locus_tag="VFK00_06060"
     CDS             complement(921..1361)
                     /locus_tag="VFK00_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592552.1"
                     /translation="MQQTFNDLVREQQEIGKGLLRFIIWMFVETSVGIARENMMSLNT
                     QAVTKSIVHALIGTGALLLIPLALQLTIGTGVDGQGFNWKPNDFVAMGVLVFCAAFGV
                     DVAARRIAGTRSRYAALAAIVLLFLLVWVHLAVGIVDSWPFAGS"
     gene            complement(1447..1815)
                     /locus_tag="VFK00_06065"
     CDS             complement(1447..1815)
                     /locus_tag="VFK00_06065"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7592553.1"
                     /translation="MVRFGQNAPLTPAVFHILLALSTRERHGYGIMKQVEADSQGAVR
                     MGPGTLYGSLKRMLEAGLVEESAGKIDPAIDDERRIYYRIAGKGRKALTAELERYRNV
                     VAVAEGKRPLPNRFVHGYGT"
     gene            complement(1936..2670)
                     /locus_tag="VFK00_06070"
     CDS             complement(1936..2670)
                     /locus_tag="VFK00_06070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7592554.1"
                     /translation="MTNLNGKTAVITGGATGIGRAAAKRFIEEGAFVFIFGRRQEALD
                     AAVADLGPNARAVKGSVTEPADLDKLYAAVKAERGTLDIVFANAGAGSQLPLGKITAE
                     HIDETFDTNVKGTIFTIQKALPLMGQGGSIILTGSSAGTTGAPGMSAYSASKAAVRNL
                     AKTWAEDLKGTGIRVNVLSPGPTATELAKAALGEEGMKVFASMNPLQRMADPAELGAA
                     AAFLASPDSSFMTASKLAVDGGLAQI"
     gene            complement(2715..3314)
                     /locus_tag="VFK00_06075"
     CDS             complement(2715..3314)
                     /locus_tag="VFK00_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7592555.1"
                     /translation="MSYAIIGFGKVGQALARAFARNNIEVAVASRRSPEELAPQAQAI
                     GPKVVPKTLQEAVKADVIFLAVLFEGHHEVGKALPNWRGKTIIDAMNSNDPVEDLDGL
                     PSSAFVAKSFPGARLVKGFNHLLAGTLATDPNIEGSHRVVFLSSDDDDAAARVADLAE
                     RLGFAPVKLGKLEEGGTLVQARGRVWGPLIFQDLFKQAA"
     gene            3441..4397
                     /locus_tag="VFK00_06080"
     CDS             3441..4397
                     /locus_tag="VFK00_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7592556.1"
                     /translation="MPVLDNVTINQLRAFVAVCDQGSFSGAARKLRRAQSAISHAIKA
                     LEGAFDVELFERNTRKAQLTAAGRSLLPDARAVISRTEEMKNRAGAIAKAGVPQVSVA
                     VDAYFPRAHLIGCLRTLQTEFPTAAMNLRVTTMQGGENLVLERVCAMAVTIEDVPEVN
                     PDALERRWLREAEMVTVCAPTHPLASTPAPIPRDEFGRHIQIVVTDNQRGAEKTQTGV
                     AGKRQWLVNDLGAKRDLLKAGLGWGHLPRHLVAEDLAGGQLVELERRAWHMRPLTFVI
                     SQRRGYDLSLFESRLVELLGDPDAARGKRMRAPSPARRKNRT"
     gene            complement(4464..5540)
                     /locus_tag="VFK00_06085"
     CDS             complement(4464..5540)
                     /locus_tag="VFK00_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HET7592557.1"
                     /translation="MNPQLFNSIRVGRRTLANRLVMAPMTRSRAKADGTPGDLAADYY
                     SQRADVGLIVTEGTQPSDDGQGYLTTPGIYTDAHVAGWKKVTAAVHDKGGRIFIQLMH
                     AGRMSHPDNTPHHRPGVAPSAIAPGAPMFTATGMQDIPEPRALTISEIHQTVEDFRFA
                     ARRAIEAGADGVEIHGANAYLVHQFFAPSANTRSDRYGGSIRNRARFAIEVATAVAAE
                     IGPDRTAIRMSPGMKLWGIDEGPEGPDLYRHLVGELDALSLAYLHVAQQGDEALLADI
                     RKLWKGALMVNRAGRPRDRIGSDLAGGLADLESYGQFVLANPDFVARMKTGAPMNAAD
                     RSTFFGGTAKGYTDYPVLREALAA"
     gene            5644..6525
                     /locus_tag="VFK00_06090"
     CDS             5644..6525
                     /locus_tag="VFK00_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7592558.1"
                     /translation="MNLNALEDFLLVATHGGLGKASRASGRSKATLSRRIADLEEALG
                     VRLLERGSNRLILTEAGQLLVARAEDPMRDVGEAVTAAREGPAKPRGRLRIAAPLLFA
                     QLAFGQLGARFLAAYPDIQLDVVAQDRAVDLVEEHFDVAIRVNPRVDSTLVGRCFARD
                     RLVIAAAPSIRRPRAGKTGKPVPIPAVVLSSYASGPWSIDRKRLVIDPQPVMRASSLL
                     IVRDAALAGVGVAVLPRSIIGSRLESGELVEWGTTGNVVELWALHTSRRLPSAKVRAF
                     MDLMRDQYPDGEFVLQG"
BASE COUNT         1130 a         2177 c         2133 g         1185 t
ORIGIN      
        1 caagggcggc cagcccggcg gccagatcgc cttctcaccg gacggcaagt acctcttcct
       61 cacggtgggt gatcgccagc gaatgacccc cgcgcaggac atcaaccagc cggaaggcaa
      121 gatcctgcgg ctgaccctgg acggcaagcc tgcgccgggc aatccgtggg ccggcaaggt
      181 cggcgcgcgc accattccgc tgatcgatcc gcccgccgat accgaggccg ccaagaccgc
      241 gccggtggtc agcacctaca cgttccccgg ccccaacctg acgcccgccg agacctgggc
      301 caccggcttt cgcacgccct acggcctcgc cttcgcaccc gacggccgcc tgtgggaact
      361 cgagcatggc ccgcgcggcg gcgacgaact gaacctcatc gagcccggca agaactacgg
      421 ctggccactg gtctcctatg gcgtcaacta caacggcgtg cccatcccca atcccgatac
      481 gcggcccgat ctcgtcaagc ccgtgatcta ttgggcgccg gtgatcgcgc cgggcaatct
      541 catgttctac aagggcaacg tctttccctg gaacggcagc gccctgatca gcgccctggc
      601 tgccgaagcc atcgtccgcg tcacgttcga cggcaagggc ggcgccaccg cggtccagcg
      661 ctgggccatc ggcatacgcg tccgtgacat cgaggaggcg ccggacggca ccctctggat
      721 gctggaagac gccaagggcg gcggcctgta ccacctcacg ccaaggtaag aaccgggtcg
      781 cgttcactca ccgggaacgg agcagaactt tctccacagg tggcaggtga acctgacccc
      841 gtgcttcggc ccagggtccg cggtggcttc atcccgctgt atcgcgatcc cgccgcgcaa
      901 ctgcgcggcg ccacggacga tcaggaaccc gcgaagggcc aactgtcgac gatcccgact
      961 gcaagatgga cccacaccag caggaacaac agcacgatcg ccgccagtgc cgcgtatcga
     1021 ctgcgcgtcc cggcaatccg ccttgccgca acgtcaacgc cgaatgcggc gcagaagacc
     1081 agtacgccca tggccacgaa atcgttcggc ttccagttga agccttgtcc atccacgcct
     1141 gttccgatgg tcaattgcag cgccaggggg atcagcagca gggctccggt gccgatgagg
     1201 gcatgcacga tgcttttggt taccgcctgc gtgttcaggg acatcatgtt ctccctcgca
     1261 attccaaccg atgtttcgac aaacatccag atgatgaaac gcaacagtcc tttcccgatc
     1321 tcctgttgct cacggacgag atcgttgaac gtctgctgca ttccatcgcc gaatcgctcg
     1381 cggtaaggct tcggataaag gcgaagcagc ccggcatacc agcgccgata gcgtgcggtt
     1441 gcgggttcat gtgccatagc cgtggacgaa ccggttcggc aacggccgct ttccctccgc
     1501 caccgccacc acattccggt agcgctccag ttccgctgtg agtgccttgc gtccctttcc
     1561 ggcaatcctg taataaatcc tgcgttcgtc atcgattgcg ggatcgattt ttccggcgct
     1621 ttcctcgacc agacccgctt ccagcatgcg tttgagcgag ccgtagagcg tgcccggtcc
     1681 catgcggacg gcgccttgcg agtcggcctc gacctgcttc atgatgccgt agccgtgccg
     1741 ctctctcgtc gagagcgcca ggagtatgtg gaacaccgcc ggggtgaggg gcgcgttttg
     1801 cccgaacctg accatgaccc caaactatat ccgcaacgga tatatgtcaa gcggtctgat
     1861 tgaaacctgg gaaaaccggg gtcagagtcg actttttgaa aagtcgactc tgaccccagt
     1921 ttctggtttc tgccgtcaga tttgcgcaag gccgccgtcg acggcgagct tgctggcggt
     1981 catgaagctg ctgtccggcg acgcgagaaa ggcagccgcc gccccgagct ccgcgggatc
     2041 ggccatgcgc tgcagcggat tcatcgaggc gaagaccttc atgccttcct cacccagcgc
     2101 tgccttcgcg agttcggtcg ccgtcggccc gggtgacagc acgttgaccc ggatgccggt
     2161 gcccttcagg tcctccgccc aggtcttcgc gaggttgcgc acggccgcct tgctcgcgct
     2221 gtaggcgctc attcccgggg cacccgtggt gccggcgctc gatccggtca ggatgatcga
     2281 accgccctga cccatcagcg gcagcgcctt ctggatcgtg aagatcgtac ccttcacgtt
     2341 cgtgtcgaag gtttcgtcga tgtgctcggc ggtgatcttg ccgagcggaa gctggcttcc
     2401 cgctccggca ttggcgaaca cgatgtcgag ggttccgcgc tcggccttca ccgccgcgta
     2461 gagtttgtcg agatcggccg gctcggtgac agaacccttc accgcgcgcg cgttggggcc
     2521 gaggtcggcg accgcggcgt cgagcgcttc ctgccggcgg ccgaagatga agacgaacgc
     2581 gccttcctcg atgaagcgct ttgccgccgc gcggccgatg ccggttgcgc cgccggtgat
     2641 tacggcggtc tttccgttca agttggtcat ttcgtggttc ctttgttgaa gttgtcgtcg
     2701 cggcggtacg ccgctcacgc cgcctgcttg aacaggtcct ggaaaatcag cgggccccag
     2761 acgcggccgc gcgcctgcac cagcgtgccg ccttcttcga gcttcccgag tttcacgggc
     2821 gcgaacccga gtcgttcggc aaggtctgcc acccgcgctg ccgcatcgtc gtcgtcgctc
     2881 gacaggaaca cgacccggtg gctgccctcg atgttggggt cggtcgccaa ggtgcccgca
     2941 agcaggtgat tgaagccctt cacgagcctg gcgccgggga aagacttggc gacaaaggca
     3001 gaggacggca gaccgtcgag atcctcgacc ggatcattcg aattcatcgc gtcgatgatg
     3061 gttttgcccc gccaattcgg gagcgctttt ccgacctcgt gatgcccctc gaacaggacg
     3121 gccaggaaga tcacgtccgc cttgacggct tcctgcagcg tcttcggaac gaccttgggc
     3181 ccgatggcct gcgcctgcgg cgccagttcc tcgggggacc gtcggctggc gacggcgact
     3241 tcgatgttgt tccgggcgaa ggcacgggcg agggcctggc ccaccttgcc gaaaccgatg
     3301 attgcgtagc tcatgggagt tctcctttca gttcgggtgt cgccggatcg acggcttgtg
     3361 tcgaatgcgg gaaccaatgt ggacctcttg acacatcaaa acaattgatg aaagattgct
     3421 aaacacaatc tagttattag atgccagtgc tggacaacgt caccatcaac caattgcgtg
     3481 cgttcgtcgc ggtctgcgac cagggaagct tctcgggtgc cgcgcggaag ctgcgacgcg
     3541 cgcagtcggc catcagccat gcgatcaagg cgctggaagg cgccttcgat gtcgaactgt
     3601 tcgaacgcaa cacccgcaag gcgcagctca ccgccgcggg gcgcagcctg ttgccggacg
     3661 cgcgcgcggt gatatcgcgc accgaagaaa tgaagaaccg cgctggcgcg atcgccaaag
     3721 ccggggtgcc ccaggtttcg gtggcggtcg acgcctactt cccgcgtgcg cacctgatcg
     3781 ggtgtctgcg gacgctgcag acggagttcc ccaccgcggc gatgaacctg cgcgtcacca
     3841 ccatgcaggg cggcgagaac ctggtgctcg aaagggtgtg tgccatggcc gtcacgatcg
     3901 aagacgtgcc ggaggtgaac ccggatgccc tggaacggcg ctggctgcgc gaggcggaga
     3961 tggtgacggt ctgtgcgccg acccatcccc tcgcctcgac acccgcaccc atccccaggg
     4021 acgagtttgg ccggcacatc cagatcgtcg tcaccgacaa ccaacgtgga gcggagaaga
     4081 cccaaaccgg cgttgccggc aagcgccagt ggctggtcaa cgatctcggc gccaagcgcg
     4141 atctgctgaa ggccggtctc ggctgggggc acttgccgcg gcacctcgtc gccgaggatc
     4201 tggcgggcgg acagctcgtc gaactcgaac gccgtgcctg gcacatgcgc cccctcacgt
     4261 tcgtgatctc gcagcgccgg ggttacgacc tctccctgtt cgagtcgcgg ctggtcgaac
     4321 tgctcggcga ccccgatgca gcacggggaa agcggatgcg tgctccttct ccggcaaggc
     4381 gcaaaaaccg gacctgattg gtgtcatatg tcggcaaaac ttgtcgtcat tcggcatggg
     4441 ccaacatcga accggaaacg taattacgcg gccaacgcct cgcgcaacac cggatagtcg
     4501 gtgtacccct tcgccgtgcc gccgaagaag gtgctgcgat cggcggcgtt catgggcgcg
     4561 ccggtcttca tccttgcgac gaaatcggga ttggcgagca cgaactggcc gtaggactcc
     4621 aggtcggcca gcccgccggc caggtcgctg ccgatccggt cgcgtgggcg cccggcgcga
     4681 ttgaccatca gcgcgccttt ccatagcttg cggatgtcgg ccagcaacgc ttcgtcgccc
     4741 tgctgcgcga cgtgcaggta cgcgaggctc aatgcgtcga gttcgccgac cagatggcgg
     4801 tagaggtccg gtccctcggg cccttcgtcg atgccccaca gcttcatgcc gggcgacatg
     4861 cggatggcgg tccggtcggg tccgatttcg gcggcaaccg ccgtggcgac ttcgatggca
     4921 aagcgggcgc ggttccggat agacccgccg tagcggtcgc tgcgcgtgtt ggcgctgggc
     4981 gcgaagaact ggtgcaccag gtaggcgttc gcgccgtgga tttcgacacc gtccgcaccc
     5041 gcctcgatcg cgcggcgggc ggcgaagcgg aagtcctcga ccgtctggtg gatttcgctg
     5101 atggtcagtg cacgcggttc gggaatgtcc tgcatgccgg tggccgtgaa catgggcgca
     5161 cccggcgcga tggcggaagg cgcgacgccc ggccggtggt gcggcgtgtt gtccggatgc
     5221 gacatgcgtc cggcgtgcat cagctggatg aagatgcggc cgcccttgtc gtgcacggcg
     5281 gccgtgacct tcttccagcc ggcgacgtgg gcatcggtgt aaatgcccgg ggtggtcagg
     5341 taaccctgcc catcatccga tggttgcgtc ccctcggtga cgatgaggcc cacgtcggcg
     5401 cgctggctgt aatagtcggc cgcgagatcg ccaggcgtgc cgtcggcctt ggcgcgcgaa
     5461 cgggtcatcg gcgccatgac caggcggttg gcaagcgtgc ggcgtccgac gcggatggaa
     5521 ttgaacagtt gcgggttcat gtgatgccct ccttgcgatg gcttgtgcac cgcaaagata
     5581 ggcagttcca ttggcaaaac acagacgcta atatgtggac acagtgtctc gatatcggaa
     5641 cacatgaatc tcaacgcgct cgaggacttc ctgctggtgg ccacccacgg cggcctgggc
     5701 aaggccagcc gcgcgagcgg ccggtcgaag gccacgctgt cgcgccgcat cgcggacctg
     5761 gaagaagcgc tgggcgtccg gctgctcgaa cgcggctcga accgactgat cctgaccgag
     5821 gccggccagt tgctggtggc ccgcgccgaa gatccgatgc gcgacgtcgg ggaagccgtg
     5881 accgcggcgc gcgaaggacc cgccaagccg cgcggccgct tgcgcatcgc cgcaccattg
     5941 ctgtttgcgc aactcgcgtt cggccagctt ggcgcccggt tcctggcggc ctatccggat
     6001 attcaactgg acgtggtcgc gcaggatcgc gcggtcgatc tggtcgaaga acatttcgac
     6061 gtggccatcc gcgtcaatcc ccgcgtagac agcacgctgg tgggccgatg ctttgccagg
     6121 gaccggctcg tcatcgccgc cgcgccctcg ataagaaggc caagggcagg caagaccgga
     6181 aaacccgtcc cgattccagc ggtggtgctg tcgtcctacg ccagcggacc ctggtccatc
     6241 gaccgcaaac gtctcgtgat cgatccacaa cccgtgatgc gcgcctcgtc gctgctgatc
     6301 gtccgcgacg ccgcgcttgc cggcgtcggc gtcgcggtat tgccgcgctc catcatcgga
     6361 tcaaggctgg aaagcggcga actggtggag tggggaacga caggcaatgt cgtcgaactg
     6421 tgggccctgc acacgtcccg tcgcctgccc agcgccaagg tgcgcgcatt catggatctc
     6481 atgcgcgacc agtacccgga tggcgagttc gtgctgcaag gttgacggtg gcgggcgagt
     6541 tttctcgcgg ctgtcgcagt tcgacccgac ggagtcatta gcgactgtct gctttgggga
     6601 ccgttgatca gcggcaagcc tcgac
//
LOCUS       DASSNH010000164         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_549717, whole genome shotgun sequence.
ACCESSION   DASSNH010000164 DASSNH010000000
VERSION     DASSNH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549717"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFK00_06095"
     CDS             <1..534
                     /locus_tag="VFK00_06095"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HET7592559.1"
                     /translation="GGWMAPLMGDRYLWKGRTHSRGFAEFRLLAELSRRGLPVPQPVA
                     ARYQRRGPYYTADLITRAIDGAATLAQLIGERRVDAPFAERVGELVARFHNDGVDHAD
                     LNAHNILLAGDRLWLIDFDRGEIRETGTAWKLGNLARLKRSLLKVGACDGDETVLDRE
                     IWMPLMRGYGRIGGNDR"
     gene            531..1352
                     /locus_tag="VFK00_06100"
     CDS             531..1352
                     /locus_tag="VFK00_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7592560.1"
                     /translation="MNRTRADTEAHAPAPTPGKRDGVWSLHVLGTGAASAVDLGSSGA
                     VLECDGEPVMLIDCGPGTLDHYLRCYGASPAAVFITHGHMDHVAGLERLFHAVWFDRQ
                     QRGRVKLFMHSNLVPILQSRVGDYPNALAEGGVNFWEGFRLVPLSRGFWLDGWWFDVF
                     ATRHHVPGTSFGVVLRGCFAWTGDTRPIPEALAAVADAQTLVAHDCALVGNPSHTGVD
                     DLEREYPEELRERLLLYHYGSEADALALRERGFKVAVPDGRYPLKAPHSVRAEAG"
     gene            1352..2398
                     /gene="moaA"
                     /locus_tag="VFK00_06105"
     CDS             1352..2398
                     /gene="moaA"
                     /locus_tag="VFK00_06105"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET7592561.1"
                     /translation="MRPEAPQALQHAPSPPDALGRALGDLRISVIDRCNFRCPYCMPE
                     EAYPEDHAFLRARDRLDFGEIERIARVFVSLGVRKLRLTGGEPLLRRDLPGLVARLAA
                     IEGVEDFALTTNGALLPRLAQPLRDAGLKRITLSMDSADPEVFRRMSGGRGELVQVEA
                     AIAAAERAGFAALKVNCVVMRGVNDDGVPDLVARFRGTGHILRFIEYMDVGTVNGWRN
                     DRVVGGAELVERIDARWPLEPLPRSPASTARRWRFRDGGGEIGFINSVTEPFCGGCTR
                     ARLSADGKLYTCLFAHAGHDLRALLRTGGSDDALRATVAGIWSHRGDRYSERRAELRA
                     AGVLEHVEMYRIGG"
     gene            2498..3016
                     /gene="moaC"
                     /locus_tag="VFK00_06110"
     CDS             2498..3016
                     /gene="moaC"
                     /locus_tag="VFK00_06110"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET7592562.1"
                     /translation="MTQRKLSHLDASNRPRMVDVGEKSPTARVAVAEAIVHFPVAVAE
                     ALRAGGMRSPKGPVIDTAIVAGTMAAKRTHELIPFCHPLAIERCRIEADFVSDTDLAI
                     RCEVAITHKTGVEMEALTGASVAALTIYDMCKALSHEIVIEGVRLLEKSGGKRTVRTG
                     KVAPRAGRQGKR"
     gene            3013..3252
                     /locus_tag="VFK00_06115"
     CDS             3013..3252
                     /locus_tag="VFK00_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7592563.1"
                     /translation="MNYRLLYFASLCDAAGRAEETVASDAQDLRSLYAELSARHGFTF
                     TPDRLRVAVNGAFADWNHRLADNDEVAFLPPVSGG"
     gene            3252..3683
                     /locus_tag="VFK00_06120"
     CDS             3252..3683
                     /locus_tag="VFK00_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET7592564.1"
                     /translation="MKGFSTSAAPIDVEVLRRELESQGAGAVVCFEGRVRDYNSGRAV
                     DGLSYQAYVELAEAEGGRIVDEARTRFAIEQVLCVHRIGDLALGEVAVWTGVSAGHRA
                     AAFDACRYVIDEVKARVPIWKREHYVEGASEWLHPQSDRSE"
BASE COUNT          623 a         1231 c         1370 g          660 t
ORIGIN      
        1 ggcggatgga tggcgcccct gatgggcgac cgctacctgt ggaagggccg cacccacagt
       61 cgcggtttcg cggagttccg actgctggcg gagttgtcgc gccgcggcct gccggtcccg
      121 caacccgtcg cggcgcgcta ccagcggcgc ggaccgtact acaccgccga cctgatcacc
      181 cgcgcgatcg atggtgccgc gacgctggcg cagctcatcg gcgagcgccg cgtcgacgcg
      241 ccgttcgcgg aacgcgtcgg cgaactggtg gcgcggttcc acaacgacgg cgtggatcat
      301 gccgacctga acgcgcacaa tatcctcctg gccggcgatc gcctgtggct gatcgacttc
      361 gatcgcggcg aaatccggga gaccggaacc gcctggaaac tcggcaatct cgcgcgcctg
      421 aagcgttcgc tgttgaaggt gggcgcgtgc gatggtgatg aaacggttct ggatcgcgag
      481 atatggatgc cgctgatgcg cggctatgga cgcatcggcg ggaacgatcg atgaacagga
      541 cacgagccga cacggaagcc catgcccccg ctccgacgcc cggtaaacgg gatggggtgt
      601 ggtcgctgca tgtgctcgga acgggggccg cgagcgcggt ggatctcggt tcgtccggcg
      661 ccgtgctgga atgcgacggt gaaccggtga tgttgatcga ctgcggcccc ggcacgctcg
      721 accactacct gcgttgctac ggcgcgtcgc cggcagcggt gttcatcacc cacggccaca
      781 tggaccatgt ggcggggctc gagcgcctgt tccatgccgt gtggttcgat cggcagcagc
      841 gcggtcgggt gaaattgttc atgcactcga acctggtgcc gatcctgcaa tcgcgggtcg
      901 gtgattatcc caacgccctc gccgaaggcg gcgtcaattt ctgggaaggt ttccggctgg
      961 ttccgctgtc gcgcggattc tggctggacg gttggtggtt cgacgtgttc gcgacgcgcc
     1021 accacgtgcc cggcacctcg ttcggcgtgg tgctgcgcgg ctgcttcgcc tggaccggcg
     1081 acacgcggcc catccccgaa gccctcgcgg ccgttgccga tgcgcagacg ctggtggcgc
     1141 acgactgcgc cctggtcggg aacccgtcgc acaccggcgt cgatgacctg gagcgcgagt
     1201 acccggaaga attgcgggag cggctcctgc tctaccacta cggcagcgag gccgacgctc
     1261 tggcgcttcg cgagcgcggc ttcaaggtcg ccgttccgga tggccgctac ccgctcaagg
     1321 cgccgcattc cgtgcgggcg gaggcgggct gatgaggccc gaagcaccgc aggcactgca
     1381 gcacgcgccg tcgccgccgg atgcgctggg tcgcgccctg ggcgacttgc gcatctcggt
     1441 gatcgatcgc tgcaatttcc gttgccctta ttgcatgccg gaggaagcgt acccggaaga
     1501 ccacgccttc ctgcgcgcgc gcgaccggct ggacttcggc gagatcgaac gcatcgcgcg
     1561 cgtgttcgtg tcgctgggtg tgcgcaaact gcgcctgacc ggtggcgagc cgttgctgcg
     1621 ccgcgacctg cccggactcg tggcgcgact ggccgcgatc gagggcgtcg aggacttcgc
     1681 gctcacgacc aacggcgcgc tgctgccgcg gctcgcgcag ccgctgcgcg atgccggcct
     1741 gaagcgcatc accctcagca tggacagcgc cgatcccgaa gtcttccgcc ggatgtcggg
     1801 cgggcggggt gaactcgtcc aggtcgaggc ggcgatcgcc gcagccgaac gcgcgggttt
     1861 cgcggcgctc aaggtcaatt gcgtggtgat gcgcggcgtc aacgacgatg gcgtgccgga
     1921 cctcgtggcg cgcttccgcg gaaccggcca catcctgcgc ttcatcgaat acatggacgt
     1981 cggcaccgtg aacggctggc gcaacgatcg cgtcgtgggg ggcgccgaat tggtcgaacg
     2041 gatcgatgcg cgctggccgc tggaaccctt gccgcgcagt cccgcatcga ccgcgcgccg
     2101 ctggcgtttc cgcgatggcg gcggcgagat cggattcatc aattcggtga ccgagccgtt
     2161 ctgcggcggc tgcacgcggg cgcggctgtc ggccgacggc aagctgtaca cgtgcctgtt
     2221 cgcgcacgcc ggacatgatt tgcgcgcact ccttcggacg ggcggatccg acgacgcgct
     2281 gcgcgcgacc gtcgccggca tctggagcca ccgcggcgac cgctacagcg agcgccgcgc
     2341 ggaacttcgc gcggcgggtg tgctggagca cgtcgagatg tatcgcatag gtgggtgaag
     2401 tttctgacgc ggatccacgc ggataaacac ggatacaaga aaaagctctt tgccttgtcc
     2461 gcgctcatcc gcgtgaaagg tttttgtcag gtgccacatg acgcaacgaa aattgtctca
     2521 tcttgatgca tccaatcgcc cacgcatggt cgacgtcggt gagaagtctc cgaccgcgcg
     2581 cgttgccgtc gcagaagcca tcgtgcattt cccggtcgca gttgccgagg cgttgcgcgc
     2641 cggggggatg cgttcgccca agggaccggt gatcgacacc gcgatcgtgg ccggcacgat
     2701 ggcggccaag cggacccacg agttgatccc gttctgccat ccgctcgcca tcgagcgctg
     2761 ccggatcgaa gccgacttcg tgtcggacac ggatctcgcg atccgctgcg aggtcgcgat
     2821 cacccacaag accggggtcg aaatggaggc attgaccggc gccagcgtgg cggcgctcac
     2881 gatttacgac atgtgcaagg cgctgtcgca cgaaatcgtg atcgagggcg tgcggctgct
     2941 ggagaaatcc ggcggcaagc gaacggtgcg gacaggaaag gtggctccgc gcgcagggag
     3001 gcaggggaag cgatgaatta ccgcctgctg tacttcgctt ccttgtgcga cgccgcgggt
     3061 cgcgccgagg aaaccgttgc gagcgacgcg caggatctgc ggagcttgta cgccgagctc
     3121 tcggcgaggc acggcttcac cttcacgccg gatcgcctgc gcgtcgcggt caacggcgca
     3181 tttgccgact ggaaccaccg tcttgccgat aacgatgagg tggcattcct gccaccggta
     3241 tccggtggct gatgaagggc ttctcgacaa gcgcggcgcc gatcgatgtg gaggtgctcc
     3301 ggcgcgagct ggaatcgcag ggcgccggtg cggtggtgtg tttcgaaggc cgcgtccgcg
     3361 actacaacag tggccgcgcg gtggacggtc tctcgtacca ggcgtatgtc gagcttgccg
     3421 aggccgaagg aggacgcatc gtcgatgagg cacggacccg tttcgcgatc gagcaggtcc
     3481 tgtgcgtgca tcgcatcggc gacctcgcgc tgggcgaagt cgcggtctgg acgggggtat
     3541 ccgcgggcca ccgcgccgcc gccttcgacg cctgccgcta cgtcatcgac gaggtcaagg
     3601 cgcgcgtgcc gatctggaag cgtgagcact acgtcgaggg ggcttcagag tggttgcacc
     3661 cccaatctga caggtcggaa tgattgccct gaagcagagg cgccggtatc ctgctgcgtt
     3721 cactttctcg gagaggtgtc cgggcggttg aaggagcacg cctggaaagt gtgtaagcgg
     3781 ctaaaccccg cttcgcgggt tcgacgaatt cgtcgggaac gaattcggac gaccgaaggt
     3841 cggccccgag caaagcgagg ggcgagcatc atggatgatg cgaa
//
LOCUS       DASSNH010000165         9151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_391170, whole genome shotgun sequence.
ACCESSION   DASSNH010000165 DASSNH010000000
VERSION     DASSNH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391170"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFK00_06125"
     CDS             <1..547
                     /locus_tag="VFK00_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET7592565.1"
                     /translation="RLHYVNFGSRDCIYVPPRDCRDGECVVAGIAKYSAILADRSNGI
                     AERAQALAFVVHFVADVHQPLHAGYRHDAGGNDFQVRWQGEGTSLHRIWDSTMLDDAH
                     LPTAAYTRKLQAEDGPVASGGTPAEWAEESCRIDRDGGVYPAIHSIDESYMRRERPIA
                     ERRLRQAGARLAALLNRDLGR"
     gene            complement(651..1115)
                     /locus_tag="VFK00_06130"
     CDS             complement(651..1115)
                     /locus_tag="VFK00_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HET7592566.1"
                     /translation="MRTINDWFASYSADHQNPVNRAIHWVCVPTIMWAVIAALWTVPV
                     ISMWMKPGFWAAVAMFLAYLFYYRLSRKLGFAMAIVFIASGVVCWALYGVLGPRGLLI
                     LAAVLFVLAWIGQFVGHAIEGRRPAFFDNIVQLLIGPAWLVGKLMRRLGIAY"
     gene            complement(1323..2219)
                     /locus_tag="VFK00_06135"
     CDS             complement(1323..2219)
                     /locus_tag="VFK00_06135"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7592567.1"
                     /translation="MPTTLLQSDLPGLELLHRGKVRDVYALPDERLLIVASDRLSAFD
                     VVLPDPIPGKGEMLTQISNFWFGKTAHLVSNHLTGKPVADALPAGVDPAPYAKRAVVV
                     KRLKPVPIEAIARGYLIGSGWKDYRATGSLCGIRLPEDLQQAQQLPEPIFTPSTKAPK
                     GTHDENIGFDQVVATIGAELAEQVRQATLAIYRFAASYAAQRGILIADTKLEFGTDAA
                     GRLFVMDEMLTPDSSRFWPADQYQVGSSPPSYDKQFVRDYLETLDWDKRAPGPKLPTE
                     VVEGTAAKYAEALKRLAGITLD"
     gene            2314..2649
                     /locus_tag="VFK00_06140"
     CDS             2314..2649
                     /locus_tag="VFK00_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7592568.1"
                     /translation="MQNARAMALILALVMPLGALAATEAPPRPPQLGLCSACHGEDGH
                     ARIPGAPNLAGQSSAYLLQALRAYRSGQRDVPVMRAAAGPLTEKDMQALANWYAAQSP
                     CKPPQAASP"
     gene            2646..3461
                     /gene="djlA"
                     /locus_tag="VFK00_06145"
     CDS             2646..3461
                     /gene="djlA"
                     /locus_tag="VFK00_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HET7592569.1"
                     /translation="MRIWGKLIGFVIGLWVTRDFGGALIGLVIGHLVFDTGWLAGLTH
                     TGGKDGYVEPLFALMGALAKSDGRVSEAEVAIVEKLMSRLDLDALWRRRAIEAFNKGK
                     APGFDLGKALVDLRSWCLPRRSSVMPFLDMLCDVALADGPLTEDKLQVLKRVAFSLRV
                     SEIQLVALLAMKGFAWHGTAGAHAGDYAHANGYTGYRAPSPRNNGPDPYAVLGVARDA
                     DQRTIKRAYRKLISEYHPDRLGNMPEDLRRRAEARASEINAAWERIQSERGFR"
     gene            3537..4205
                     /gene="rpe"
                     /locus_tag="VFK00_06150"
     CDS             3537..4205
                     /gene="rpe"
                     /locus_tag="VFK00_06150"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7592570.1"
                     /translation="MAKQSCVIAPSILSANFAKLGAEVDAVIDAGADWIHFDVMDNHY
                     VPNLTIGPMVLKALRDYGITAPMDVHLMVEPVDRIVPDFAEAGASSISFHPEATRHVD
                     RTLSLIREHGCKAGLVFNPATPLDWLDYVLDKVDIVLVMSVNPGFGGQKFIASALEKL
                     KRAREIIDRSGRPIHLEIDGGVKPDNIAEIARAGADTFVAGSAIFGSDDYGKTIAAMR
                     AAVG"
     gene            4842..5531
                     /gene="pdsR"
                     /locus_tag="VFK00_06155"
     CDS             4842..5531
                     /gene="pdsR"
                     /locus_tag="VFK00_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HET7592571.1"
                     /translation="MARAIAIVEDEPSIRANYVEALSRIGYDVRGFASRAEASGAFSN
                     RMPELVIIDIGLGDEPEGGFDLCRELRARSATLPIIFLTARDSDFDVISGLRLGADDY
                     LSKDISFHQLAARIGALFRRAESQKTPAAAETVVEHGPLKLEAERMRVTWNGVEVPLT
                     VTEFWMVHTLLRFPGHVKNRDQLMRDAELVVDDATITSHIKRIRKKFLAVDPAFDAIE
                     TVHGVGYRWRV"
     gene            5723..7714
                     /locus_tag="VFK00_06160"
     CDS             5723..7714
                     /locus_tag="VFK00_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7592572.1"
                     /translation="MTLRRKLLLVALSTLALPWAGWLYVRQMEHLLRNSQEQALLAIA
                     FALDRGLVAVGAELPNDDRNWYIQQATRPIVVDGGGRDWSAMAGWAQDPWPGVEVQFA
                     HDPAWLYLRVRVADATPTRLDAGDSAAVETDHVVLELVRGNSQRSYLLASAASGAFLA
                     RPLDPRVHGLPDEISGQWQDDGNTYQIELRMPRAQAPARLGIGVFDANAPERFGGGKP
                     DVRPLLSLSPALSNDLTQLAPSGVRVRLLSADGWVIGEGGSMLDSPKPEQSRFPWLQR
                     VLSDLLVVPSLDRRQRLPRDIPRLSEPDVQQALDGQATANWRGGDEPGSVVLTVSVPL
                     DVRGQNRGALVLEQSSQALPLLTNRALLGLMLASIVVLLIAGAVLFAFATWLSVRIGR
                     LRYAAERAQYHEGLVVARLPLVDDHDELGDLARSLARLLESVGTYTEYLRKLASNLSH
                     ELNTPLAIVRSSLENLEHAELPHEALPYLARARDGAARIGTIVRAMSEASRMERAMAS
                     ADGEDMDLAQVVRGCVEAYRALAGQRELAFSAPQRPVPLYGAPELIAQALDKLFDNAL
                     SFTPADGWIRIALEAEESGGACIKVSNRGSQLPESMQGQLFDSLVSVRGSGARAGTPH
                     LGLGLYVVRLVAELHQGKVSARNDPDGVTFEMELRGMRE"
     gene            7878..>9151
                     /locus_tag="VFK00_06165"
     CDS             7878..>9151
                     /locus_tag="VFK00_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET7592573.1"
                     /translation="MIDQTEFDALARDGHNRIPLVREVLSDLDTPLSVYLKLADGPYT
                     FLFESVEGGANWGRYSIIGLPARRVFAFHGHTLTVSEDGVAIESRDVADPLTEIERLR
                     AEYRVPHLPDLPAFTGGLVGYFGFETVGYIEPRLARFDAPDELGTPDVLLMLAEEVAV
                     FDNLKGRLYLIVHADTARPHAYARAQRRLDALAHRLRQGGAGYPPVVKGAMLDEGDFK
                     SSFTREAFEAVVERCKEYIRAGDVFQVVPSQRLGIGFRARPVDVYRALRALNPSPYMY
                     FVDLGDTQIVGSSPEILARFKDGKVIVRPLAGTRRRGATPDEDVVLEKELLADPKERA
                     EHLMLIDLGRNDVGRVSKTGTVRVSDSFAIERYSHVMHIVSQVEGVARPGTTYMDVLR
                     ATFPAGTLSGAPKIRAIEIIQELEPFKRNIYSG"
BASE COUNT         1517 a         2993 c         3126 g         1515 t
ORIGIN      
        1 caggttgcac tacgtcaatt tcgggtcccg cgattgcatc tatgtaccgc cgcgcgactg
       61 ccgcgacggc gagtgcgtcg tggccgggat cgccaagtac tcggcgatcc tggccgaccg
      121 ctcgaacggt atcgccgagc gggcgcaggc gctggcattc gtggtccact tcgtcgcaga
      181 cgtccaccag ccgctgcacg cgggctatcg ccacgacgcc ggcggcaacg atttccaggt
      241 gcgctggcag ggcgaaggga ccagcctcca ccggatctgg gattcgacga tgctggacga
      301 cgcgcatctc ccgacggcgg cgtacacgcg gaagctgcag gccgaagatg ggccggtcgc
      361 gtcggggggc acgcccgcgg aatgggccga ggagtcgtgt cgaatcgatc gcgacggggg
      421 ggtctatccg gccatccatt ccatcgacga gagctacatg cggcgcgagc gcccgatcgc
      481 ggaacgccgg ctgcggcagg cgggcgcgcg cctggcggcg ctgctgaacc gggacttggg
      541 ccggtaggtt gggctggatc cggattcaac cggaagaagc ccaaccttgc gacggtatgc
      601 cacgttgggc ttcccgcgga tgaggcccgc ggtcagccca acctgcatct tcaataggcg
      661 ataccgagcc ggcgcatcag cttgccgacc agccacgccg ggccgatcag caactgcacg
      721 atattgtcga agaatgccgg ccggcgtccc tcgatcgcgt gtccgacgaa ctggccgatc
      781 caggccagca cgaacagcac ggctgccagg atcagcagcc cgcgcggtcc aagcacgccg
      841 tacagcgccc agcaaaccac gccgctcgcg atgaacacaa tcgccattgc gaagcccagc
      901 ttgcgcgaca accggtaata gaaaaggtag gcgaggaaca tcgcgaccgc cgcccagaat
      961 ccgggcttca tccacatcga aatcaccggc accgtccaaa gcgccgcgat caccgcccac
     1021 atgatggtcg gcacgcagac ccaatggatc gcgcggttga ccggattctg gtggtcggcg
     1081 ctgtagcttg cgaaccagtc gttgatcgtg cgcatgctgc acttccggat cgttcgcgtg
     1141 gagcgtagcg tgccgcgcgg tcgaaatcga cggcgacccg cagcgcgtgc ggcccggatc
     1201 catgcccgtc ggatgccgga gcttccgaag cgtcccgccg ctgtcgcgaa gccgagggcc
     1261 tgcaatgtcc ggagggattc acccggcgga cgatgtccgt cctgcgggga tgtggcgccg
     1321 gatcagtcca gcgtgatccc cgccaggcgc ttcagcgcct ccgcgtattt cgcggcggtg
     1381 ccttcgacca cctcggtcgg caacttcggg ccgggtgcgc gcttgtccca atccagcgtc
     1441 tcgagatagt cgcgcacgaa ctgcttgtca taggacggcg ggctgctgcc gacctggtac
     1501 tgatccgccg gccagaagcg cgaggagtcg ggggtcagca tctcgtccat cacgaacagc
     1561 cgtccggcgg catcggtgcc gaattcgagc ttggtgtcgg cgatcaggat gccacgttgc
     1621 gcggcatagc tcgcggcgaa gcggtagatc gcaagcgtgg cctggcgcac ctgctccgcg
     1681 agttcggcgc cgatcgtggc gaccacctgg tcgaaaccga tgttctcgtc gtgcgttccc
     1741 ttgggcgcct tggtcgaagg cgtgaagatc ggctcgggca gttgctgcgc ctgttgcagg
     1801 tcctcgggca gccggattcc gcacagggag cccgtcgccc ggtaatcctt ccagccggaa
     1861 ccgatgaggt agccgcgcgc gatcgcttcg atcggcaccg gcttgaggcg cttcacgacc
     1921 accgcgcgct tcgcgtacgg ggccggatcg acgcccgccg gcaacgcgtc cgccaccggc
     1981 ttgccggtca gatggttgga cacgaggtgc gcggtcttgc cgaaccagaa gttggaaatc
     2041 tgcgtcagca tctctccctt gccggggatc gggtcgggca gcaccacgtc gaaggccgac
     2101 aggcggtcgc tcgcgacgat cagcaggcgc tcgtccggca gcgcgtacac gtcgcgcacc
     2161 tttccgcggt gcagcagttc cagcccgggc aaatccgatt gcaaaagggt ggtcggcatg
     2221 gtgtttccgg catcgctcgc gaaaccgcca ttgtagtcgc gagggggcgc gcgggttgag
     2281 cgctggcggc gccccggctg ataaactggc cggatgcaaa atgcacgcgc catggccctg
     2341 atcctcgccc tcgtcatgcc gcttggggca ctcgcggcga cggaagcgcc gccgcgtccg
     2401 ccgcagctcg ggctgtgctc ggcgtgccat ggcgaggacg gccacgcgcg gattcccggc
     2461 gccccgaacc tcgccgggca gtcctccgcg tacctgctgc aggccctgcg cgcctatcgc
     2521 tcgggccagc gcgacgtgcc cgtgatgcgg gcggcggcgg gtccccttac cgagaaggac
     2581 atgcaggcac tggcgaactg gtacgccgcc cagtcgccgt gcaagccgcc gcaggcggcg
     2641 agcccatgag gatctggggc aagctgatcg ggttcgtgat cggtttgtgg gtgacgcgcg
     2701 acttcggcgg agcattgatc ggcctggtga tcggacacct ggtgttcgac acgggctggc
     2761 tggccggact cacccacacc ggcggcaagg acggctacgt cgaaccgctg ttcgcgttga
     2821 tgggtgcgct cgccaagagc gacggccggg tctcggaagc cgaagtcgcc atcgttgaga
     2881 agctgatgtc gcgcctcgac ctggatgcgc tgtggcggcg gcgcgcgatc gaagccttca
     2941 acaagggcaa ggcgccgggc ttcgatctcg gcaaggcact ggtcgacctg cgttcctggt
     3001 gcctgccgcg ccgcagctcg gtgatgccgt tcctcgacat gctgtgcgac gtggcgctgg
     3061 ccgacgggcc gctcaccgag gacaagttgc aggtgttgaa acgggttgcg ttctcgctgc
     3121 gcgtcagcga aatccagttg gtggcgctgc tggcgatgaa gggcttcgcc tggcacggca
     3181 ccgccggcgc gcatgcggga gactacgcgc acgcgaacgg gtatacgggt tatcgcgcgc
     3241 catcgccgcg caacaacggt cctgatccct atgcggtgtt gggcgtggcg cgcgatgccg
     3301 accagcgcac catcaagcgc gcctaccgca agctgatcag cgaataccat cccgaccgcc
     3361 tcggcaacat gccggaagac ctgcgccgtc gcgccgaagc ccgtgccagc gagatcaatg
     3421 cggcgtggga gcgcatacaa tcggagcggg gattcaggta ggagcgcgat gcgcgcgatt
     3481 cctggcctca ccgtctgcaa ggtcgccggc atggccggct cccacgaggg caacccatgg
     3541 cgaaacaatc ctgtgtgatc gcgccttcga ttctttcggc caacttcgcc aaactcggcg
     3601 cggaagtcga tgccgtgatc gatgccggtg ccgactggat ccatttcgac gtgatggaca
     3661 accattacgt cccgaacctc accatcggcc caatggtact gaaggccctg cgcgactacg
     3721 gcatcaccgc accgatggac gtgcacctga tggtcgagcc ggtggaccgg atcgttcccg
     3781 acttcgcgga ggccggcgcg agttcgatca gtttccaccc ggaggccacg cggcacgtcg
     3841 accgcacgct gtcgctgatc cgggagcacg gctgcaaggc ggggctggtg ttcaatcccg
     3901 ccacgccgct ggactggctg gattacgtcc tcgacaaggt cgacatcgtg ctggtgatgt
     3961 cggtgaaccc cggcttcggc ggccagaagt tcattgcctc ggcgctcgaa aaattgaagc
     4021 gtgcgcgcga aatcatagac cgtagcggcc gtccgatcca tctcgaaatc gacggcggcg
     4081 tcaagccgga caacatcgcg gaaatcgcgc gcgccggcgc cgacaccttc gttgccggat
     4141 ccgccatttt cggcagcgac gactacggga agaccatcgc ggcgatgcgc gcggccgtcg
     4201 gttgaatcct ggttgggggc aacgccgtcc gcaaacgaac gcgaatcagt cagaatcgca
     4261 cgctgtagtt atggatgtgc gatcgaagcg gccgttgtga aaagtcagcc catggatggg
     4321 ggtactggat tcgccgaacg ttgaagcgtg caatctcgcg attcgtcgat cggagccgcg
     4381 ttcacggacg aacgcacaaa caaaggcccc ggtgcacgag caccggggcc aaagtcgacc
     4441 aggagaggaa tcaagattcg ccggagccgc gaggctccca accatgtggt ggtccgccgc
     4501 gctggacacc ttaccggtcg tgagagaccg gttgcgtctc cggtgccagc gcggaggtgt
     4561 tccactgtcg ctaatccacg gcaggagtat cgacgcgatc cattgggcgc cccgggggcg
     4621 ccgcgcgggt ggcgcggttc ggcccgaaac ggggcaattt gtggcaaaac acgggcagcg
     4681 ggtcgccccg ggttgcgcgc cgcgaccacc tggcgcacgc tcctgcggcg gatcgggtag
     4741 cccggatgcg ggagcgcggc gctgcgatcc gggagtccgc cgaggaatgt ttccggatga
     4801 cgctcctctc catccgggct gcgaattgac tggagaattg cgtggcacga gcgatcgcga
     4861 tcgtcgagga cgaaccctcc atccgcgcga actacgtcga ggcgctgtcg cggatcggct
     4921 atgacgtgcg cggtttcgcc tcgcgagcag aagcctcggg ggctttctcc aaccgcatgc
     4981 ccgagctcgt catcatcgac atcggcctcg gtgacgagcc cgagggcggg ttcgacctgt
     5041 gccgggagct gcgcgccagg tcggccacct tgccgatcat tttcctgacc gcgcgcgatt
     5101 cggatttcga cgtgatttcg ggcctgcgcc tcggggccga cgattacctc tccaaggaca
     5161 tcagcttcca ccagcttgcc gcgcggatcg gtgcactgtt ccggcgcgcg gaatcgcaga
     5221 agaccccggc cgccgccgaa accgtggtcg aacacggccc gctcaaactc gaggccgagc
     5281 gcatgcgggt gacctggaac ggcgtcgagg tcccgctgac ggtcacggaa ttctggatgg
     5341 tgcacacgct gctccggttt cccggccacg tgaagaaccg cgaccaactg atgcgcgatg
     5401 ccgaactggt cgtggacgac gccaccatca cctcgcacat caagcgcatc cgcaagaagt
     5461 tcctcgccgt cgacccggct ttcgacgcga tcgagacggt gcacggggtg gggtaccgct
     5521 ggagagtcta gttgtgcgtt cgtccatgaa cgctgcgcgt cgaggcgcta caacggcgtg
     5581 acgggcagtg ccatcggctg caccagaacg gccatccatg gcctgacttt ccacaacagc
     5641 ggcttcgatc ggtacattga tgcagccagc cccggctaag ctgctcccgt tccccattcc
     5701 ccgttccctg ttcccgctcc caatgacgtt gcgacgcaaa ttgctgctgg tcgcgctgtc
     5761 gacgctggca ttgccgtggg cgggctggct gtacgtgcgg cagatggagc accttctgcg
     5821 caacagccag gagcaggcgc tgctggcaat cgcgttcgcg ctcgaccgtg gcctggtcgc
     5881 ggtgggtgcg gaactgccga acgacgaccg gaactggtac atccagcagg cgacgcggcc
     5941 gatcgtggtg gacggcggcg gacgcgactg gtcggcgatg gcgggttggg cgcaagaccc
     6001 gtggccgggc gtcgaggtgc agttcgccca cgatcccgcc tggttgtact tgcgtgtcag
     6061 ggtggcggac gcgaccccca cccggctgga cgccggcgat tctgcagccg tcgagaccga
     6121 tcacgtcgtg ctggaactgg tacgcgggaa ttcgcagcgg agttacctct tggccagcgc
     6181 ggcctccggg gcattccttg cgcggccgct ggatccgcgg gtgcacgggt tgccggacga
     6241 aatcagcggg caatggcagg acgacggcaa tacctaccag atcgagctgc ggatgccgcg
     6301 cgcgcaggcg ccggccaggc tcggcatcgg cgtgttcgac gccaatgcac cggaacggtt
     6361 cggcggcggc aaaccggacg tccgccccct gctcagcctt tcgcctgcac tttcgaacga
     6421 cctcacccag ctcgcgccca gcggcgtacg cgtgcgcctg ctcagcgccg atggctgggt
     6481 gatcggtgaa ggcggcagca tgctcgattc gcccaagccc gagcagagcc gcttcccgtg
     6541 gctgcagcgg gtattgtcgg accttctggt ggtgccgtcg ctggaccggc gccagcgctt
     6601 gccccgggat atcccgcgat tgtcggaacc cgacgtgcag caggcgctgg acgggcaggc
     6661 gaccgccaac tggcggggcg gcgacgagcc cggcagcgtg gtgctcaccg tttcggtgcc
     6721 gctcgacgtg cgcggccaga atcgcggcgc gctggtgctg gaacaatcga gccaggccct
     6781 gccgctgctg accaaccgcg ccttgctggg cctgatgctc gcgagcatcg tggtgctgct
     6841 gatcgcgggc gcggtactgt ttgccttcgc cacctggttg tcggtacgga taggccgcct
     6901 gcgctacgcc gccgaacggg cgcagtacca cgaggggctc gtcgtggcgc gcctgcctct
     6961 ggtcgacgac cacgacgaac tgggcgatct cgcgcgcagc ctggcgcggc tgctggaatc
     7021 ggtcggcacc tacacggagt acctgcgcaa gctggcgtcg aacctgtcgc acgagttgaa
     7081 cacgccgctc gcgatcgtgc gctcgtcgct ggagaacctc gagcacgccg agctgccgca
     7141 cgaagcgttg ccctacctcg cgagggcgcg tgacggtgcg gcgcggatcg gcacgatcgt
     7201 ccgcgcgatg agcgaagcca gccgcatgga gcgagcgatg gcgtcggccg atggcgaaga
     7261 catggatctc gcgcaagttg tgcgcggctg tgtcgaggcc tatcgcgcac tggcggggca
     7321 gcgcgaactg gccttctcgg ccccgcaacg tccggtgccg ctgtacggcg cgccggaact
     7381 gatcgcgcag gcgctggaca agctgttcga caatgcgttg tcgttcacgc cggcggacgg
     7441 ctggatccgc atcgcgctcg aagcggagga atcgggcggc gcgtgcatca aggtctccaa
     7501 ccggggctcg cagcttccgg aatcgatgca ggggcagttg ttcgattcgc tggtgagcgt
     7561 gcgcggcagc ggggcgcgcg cgggaacccc gcacctcggc ctcggactct acgtggtgcg
     7621 attggtcgcg gagctgcacc agggcaaggt ctcggcgcgc aacgacccgg atggcgtcac
     7681 cttcgaaatg gagctgcgcg gcatgcgcga atagtcgtgc gttcgtccat gaacgctgca
     7741 tcaaggtgcg ctcggttttt caaagctcct cgcggtaggg gagaccaaca cggccatcca
     7801 tgggctgact ttccacaaca gctgcttcgt gacaggcgtt gtttgcggta cgttggaaaa
     7861 tccatcgagg aatcgcagtg atcgaccaga ccgaattcga cgcgctcgcg cgcgacgggc
     7921 acaaccgcat tccgctggtg cgcgaggtgc tgtcggacct cgacacgccg ctgtcggtgt
     7981 acctgaagct cgcggacggg ccgtacacct tcctgttcga atcggtggag ggcggcgcga
     8041 actggggacg ctattcgatc atcggtcttc cggcgcggcg ggtattcgcg ttccacggac
     8101 acacgctgac ggtgagtgag gatggcgtgg cgatcgagag ccgggacgtg gccgatcctc
     8161 tcaccgaaat cgagcgcctg cgcgccgagt accgcgtgcc gcacctgccg gacctgccgg
     8221 cgttcaccgg cgggctcgtg ggttatttcg gattcgaaac cgtcggctac atcgagccgc
     8281 ggctggcccg cttcgacgcg cccgacgagc tcggtacgcc cgacgtgctg ctgatgctgg
     8341 ccgaggaagt cgcggtgttc gacaacctca agggccggct ttatctgatc gtgcacgccg
     8401 acaccgcgcg accgcacgcg tacgcgcgcg cccagcggcg actggatgcc ctcgcgcacc
     8461 gcttgcggca gggcggggcg ggctatcccc cggtcgtgaa gggagcgatg ctggacgaag
     8521 gcgatttcaa gtcctcgttc acgcgcgaag ccttcgaggc tgtggtcgag cgctgcaagg
     8581 aatacatccg cgccggcgac gtgttccagg tcgtgccctc gcagcggctc ggcatcgggt
     8641 ttcgcgcgcg gccggtggac gtctatcgcg cgctgcgcgc gttgaatccc tcgccctaca
     8701 tgtacttcgt ggacctcggc gatacccaga tcgtgggttc ctcgccggaa atcctcgccc
     8761 gcttcaagga cggcaaggtg atcgtacggc cgctcgcggg aacccgccgg cgcggcgcga
     8821 cgcccgacga ggacgtggta ctcgaaaagg aattgctggc cgatcccaag gagcgcgccg
     8881 agcacctgat gctgatcgac ctcggccgca acgacgtcgg ccgcgtcagc aaaacgggta
     8941 cggtgcgcgt cagcgactcg ttcgcgatcg aacgctactc gcacgtgatg cacatcgtct
     9001 cgcaggtcga aggcgtggcg cggccgggga ccacttacat ggatgtcctg cgcgcgacgt
     9061 tccccgccgg cacgttgtcc ggcgcgccca agatccgcgc gatcgagatc atccaggaat
     9121 tggagccctt caagcgcaac atctattccg g
//
LOCUS       DASSNH010000166        27915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1018173, whole genome shotgun sequence.
ACCESSION   DASSNH010000166 DASSNH010000000
VERSION     DASSNH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 27915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27915
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018173"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1214
                     /locus_tag="VFK00_06170"
     CDS             120..1214
                     /locus_tag="VFK00_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7592574.1"
                     /translation="MRQRPIHLRRYAVPVVLAALLAACGASRHPPQSPQPSLATIAVQ
                     PRQAAREQTWDGVVEAVDATTIAAQTNARVLELPLDVGDRVRKGDVLVRFSDVEQQSG
                     RRAASANVAAARAELRDAEANWKRTDAIFQRGLIARAQLDAAVARRDSARAALAAAEA
                     ALRSAGQQADYTVLRAPFDGVITQRFVHVGEAVQSGPPTPQPLIALAALDALRVDVVV
                     PQGAVAAIRASPRASVVLDDGKRVQAARVTVFPYADPGTHSFRVRVELPAGGSGLYPG
                     MTVKAAFAVGQATRLLVPVGALVRRGELSAVYVVGADHSVGLRQLRLGHRIGDEVEVL
                     AGLAPGDHVARDPEAAALYLTRQHGGNASP"
     gene            1211..4426
                     /locus_tag="VFK00_06175"
     CDS             1211..4426
                     /locus_tag="VFK00_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7592575.1"
                     /translation="MNAPRLGFAGRVARVFQDHPLTPILALAALLIGFTALLITPREE
                     EPQIDVTMANVIVPFPGADVHDVENLVASPLEQKLAGIDGIKHVYSASRPGVALLTVE
                     FKVGVPRQTALVRLYNQVFQNRDLWPPALGVGQPMVRAMGIDDVPVLALTLWIDDPAR
                     GPIDLAEVAHTLETDLKHIPGTRDVYTLGAPDRAVVVQVDPAKLAAYGMAVDDLAQSL
                     RAANVVTQAGDRVAGDHDVPVTAGTFLGGADDIAQLVIGLKGGQPVFLSDVATIRRGA
                     DLPDRYVWFGTPPGKGGPATGAAPAVTMVVTKLSGTNAADITQAIRHRLDALEGVVIP
                     AGVHVIVTRDYGETSTAKADLLMHKLLFATLSVVLLVLLTLGWREAIVVGSAVVITLM
                     LTLFASWAMGFTINRVSLFALIFSIGILVDDAIVVVENIHRHMALGGRSLREAIPEAV
                     SEVGGPTILATFTVIAALLPMAFVSGLMGPYMRPIPINASAGMLLSLAVALVVTPWLA
                     LKLVRRHASAPAHDDGPRETRLLRLFRRVMTPFLGGGRAPSRRRLLFASMGVLVLAAV
                     GLVVVKAVVLKMLPFDNKSEFQVVVDMPEGSTLEATNALLGQLAHALDRVPEVMDYQG
                     YAGTAAPITFNGLVRQYYLRSGPIDGDLQVNLVDKSDRDRKSHEIALAVRPMLAKIGD
                     RFGASIKVVEVPPGPPVLAPIVAEIYGPDYATLRKLARGLEKDFRSDPDIVDIDTSVE
                     ADSPRAVVVVDRARAARLGLDQAQIAQALRFALSGDDATYLMDGRARYAVPVRLRLPP
                     GNQAALEQLLSLRVRAKSGALVPLSAVASVREPGWEKAIYHKDLLPYTYVTGDGAGRT
                     DSPLYGMFAMVAKLRHGEADGHQLSQNFVHQPVDPAGYAVKWDGEWQITYETFRDMGI
                     AYAAGLVLIYLLVVGHFRRYMVPLIIMAPIPLTVIGVMPGHALLGAQFTATSMIGMIA
                     LAGIIVRNSILLIDFIDLELAKGRPLAEAVIEAAAVRAKPIALTALAAMIGGFFILDD
                     PIFQGLAVSLIFGILVSTVLTLLVIPLLYYAWLARAGGGHP"
     gene            complement(4458..5231)
                     /locus_tag="VFK00_06180"
     CDS             complement(4458..5231)
                     /locus_tag="VFK00_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592576.1"
                     /translation="MPASPCRHTVIVAIAAANPLIAVKNAADPGTTERDARQDRKLLT
                     TINKRCRRDATVGLLPEPGKVNVMPRPDARDRIDHSPRAASPPGMGWNQRLLDGTLVH
                     IRPIRKQDAGLELEFLNGLSSEMRGLRFLGLIREPGPDVARGLTDLDPRCAAGFIAVV
                     SDGGRERQIGAAHFHVSPKGDSCDCSVTVSDEWQKRGVGSSLMHVLIDAARQRGIRHL
                     RAFAPAQSGGSHRLASRLGFKRHLDLHDPSVEAYHLELT"
     gene            5230..5469
                     /locus_tag="VFK00_06185"
     CDS             5230..5469
                     /locus_tag="VFK00_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592577.1"
                     /translation="MEFHIRIRGASPNLDAINAALLDVDPTAVADIEPGGASLRVSVA
                     LGSPELIALLTQAGYPVDWKQLEEVPSVCCGACGG"
     gene            5533..6003
                     /locus_tag="VFK00_06190"
     CDS             5533..6003
                     /locus_tag="VFK00_06190"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7592578.1"
                     /translation="MGDEQSFEVSLEQVEDFEFRVHFDGTALEDLSTDEPPPQGRNAG
                     PNPARMLLVAVANCLAASLLFSLRKFRNAPGPLRAKARGRLARNAQGRWRVAGMEVDL
                     QLADPAASLQHVERALAQFEDFCIVTESVRAGIPVTVRVHDGEGREVHATGNAA"
     gene            complement(6090..7181)
                     /locus_tag="VFK00_06195"
     CDS             complement(6090..7181)
                     /locus_tag="VFK00_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HET7592579.1"
                     /translation="MSGPAPNSFAPAPHGFIAHLRASIETMSPGYFSMVMATGILSLA
                     SHLLGLPLLAKALFALNAVLFAIICILAVARLAWHRRRTLGDLFDHLQAPGFFTFVAA
                     CSILGSQALLIGGSARIAAAFGVVALLAWVGLTYTIFTVLTVKQQKPALDRGINGGWL
                     LAVVATQSLAVLGAQLAPEVAQPLRMELNFFALSMWLWGGMLYIWMMSLIFYRYTFFA
                     FSPADLSPPYWINMGAMAISTLAGSLLIENTPHAPFLGSMLPFLKGFTVFYWATGTWW
                     IPMLAVLAIWRHGIKRFPFTYDPMYWGAVFPLGMYATATLRMMHAMDLPFLGLLPHFM
                     FAAGCAAWLLLFAGQLRALRRDWRAYARG"
     gene            complement(7207..7926)
                     /locus_tag="VFK00_06200"
     CDS             complement(7207..7926)
                     /locus_tag="VFK00_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7592580.1"
                     /translation="MSITINGVTVDPADWPTPQLAAVHELLRQRASELGFITDDTSEA
                     DTERAIERALAEEVPVPEPHEEECRRWYESNQQRFRSGDLVCARHILFQVTPGAPVPA
                     IRAVAEAMLHEIRSNPALFGERAREHSNCPSGANGGNLGQLQRGETLPEFEKAIFGDD
                     AGIGILPKLVTTRHGFHIISVDRRIEGRQLPYDIVRDDVAAELRRRSEARALSQYVRV
                     LAGNADIEGVELDAAASPLVQ"
     gene            complement(7923..8630)
                     /gene="narI"
                     /locus_tag="VFK00_06205"
     CDS             complement(7923..8630)
                     /gene="narI"
                     /locus_tag="VFK00_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HET7592581.1"
                     /translation="MTLPAPLNFFLFGIYPYICLAVLLLGSLIRFDREPYSWKSDSSQ
                     TLYKRELRLGSNLFHYGVLVVILGHFVGFLMPESLVLWLMSPKQHQLLAMVVGGIAGL
                     IAIVGVSILIHRRLYYRRIRGNSRKWDIAIVFILWLQLALGLATVPISAQHMGSGEFE
                     RIVSYVQGVVYFHRDIAGIMQGLAWPYQMHVLLGFTIFLVSPFTRMVHIWSGVGSLAY
                     LVRPYQLVRKRGGSVNT"
     gene            complement(8650..9294)
                     /gene="narJ"
                     /locus_tag="VFK00_06210"
     CDS             complement(8650..9294)
                     /gene="narJ"
                     /locus_tag="VFK00_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HET7592582.1"
                     /translation="MRTRDPSLLLRALSALLAYPDTELRAALPEIAGAIRDARAVDAS
                     LREQLVVLVDEIAASDPLEAEGRYVDLFDRGRRTSLNLFEHVHGDGRQRGPAMLELKQ
                     RYLDAGMEPVADELPDHLPLLLEYLSCRDAGEVRDTIGEIAHILRQLGNTLLKKHSRY
                     AAVMAALLALGGEQGLDAHAAVPPAEDLDQAWEERPAFAPPEPNPAVTPEHPTS"
     gene            complement(9291..10832)
                     /gene="narH"
                     /locus_tag="VFK00_06215"
     CDS             complement(9291..10832)
                     /gene="narH"
                     /locus_tag="VFK00_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HET7592583.1"
                     /translation="MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSRKGMEYAWFNNV
                     ETKPGIGYPKDWENQERWKGGWERKSNGRLVPKQGGRWAVLAKIFANPHLPEIDDYYE
                     PFTFDYENLQTAPELPTAPVARPISLVSGRTMDKVEWGPNWEEILGGEFDKRSKDYNF
                     EAVQKDIYGQFENTFMMYLPRLCEHCLNPTCVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWVSGKAEKCTFCYPRIEAGLPTVCSETCVGRIRYLGVLLYDA
                     DRIEQAASTENPMDLYQAQLDVFLDPSDPEIQRQALADGIPAAWIESAQKSPAYKMAV
                     EWKIAFPLHPEYRTLPMVWYVPPLSPISAHANSGNIDSAANGLPDVGSLRIPVRYLAN
                     LLTAGNEPPIVSALERMLAMRAWFRGKQLGNAPDAALLSGVGLDEATANDMHRYLAIA
                     NYEDRFVIPTAHREEAENAYQLRGGCGFTFGNGCDGIDAKAGLFGGKMGRRKVIPIRN
                     IDDHA"
     gene            complement(10832..14602)
                     /locus_tag="VFK00_06220"
     CDS             complement(10832..14602)
                     /locus_tag="VFK00_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HET7592584.1"
                     /translation="MGQFLERLRYFKRPREPFSGGLGELRTEARQWEDGYRNRWSHDK
                     VVRSTHGVNCTGGCSWKIHVKQGVVTWETQQTDYPRTRPDMPNHEPRGCSRGASYSWY
                     LYSANRLKYPMVRGRLLKRWRQARKSLQPVQAWAALMADPDARDYKAARGLGGFVRAK
                     WDEVLEIVAAANVYTIKHHGPDRVVGFSPIPAMSMVSFASGSRYLNLIGGSLLSFYDW
                     YCDLPPSSPQTWGEQTDVPESADWYNSGYIIAWGSNVPQTRTPDAHFFVEARYNGTQI
                     VAVTPDYSEVAKLSDLWLHPKQGTDAAAAMAMGHVILKEFFIDRQVPYFQDYCRKYTD
                     LPLLVTLRRDGARWVPDRYLRASDFEGALGSPGNADWKTVGFDKDGQPVVPTGSIGFR
                     WPQGDSLDHGKWNLEEKSGDGRDIELQLTQIDRRDDVADVAFPYFGGVANPHFTANTQ
                     GADGLLIRRVPVRKLATKNGETMVATAFDLLCAHYGLDRGLGGECAKNYDDNVAYTPA
                     WQEAITGTPAAHVIEVARGFAHNAEKTRGRSMVIIGAGMNHWYHQDMNYRSIINFLML
                     CGTVGVSGGGWSHYVGQEKLRPQTGWTALAFALDWVRPPRHMCGTSFFYAHSDQWRYE
                     TLKTAEQLSPLADASRYGRTLIDCNVRAERMGWMPSAPQLDRNPLDVARDAAAAGKDA
                     KDYVAAALKSGDIRMACEDPDNPVNFPRNLFIWRSNLFGSSGKGHEYFLKHFLGTRCG
                     LQGKDLGEDGGVKPEEVAWHDPAPEGKLDLVVTLDFRMSTSCLYSDVVLPTASWYEKN
                     DLNTTDMHPFIHPLSAAVDPVFESKSDWEIFKAVAKKFSELSEGVLGIEKDVVLVPIQ
                     HDTPAEIAQPFEVRDWKKGECELVPGVTAPNIVTVERDYPATYARYTSLGPLMDKLGN
                     GGKGIAWNTEHEVKGLAALNYTVADGPAKGRPRIFSDIDAAETIMYLAPETNGEVAVK
                     AWAALSKITGRDHTHLARAREDEKIRYRDIQAQPRKIISSPTWSGIESEHVSYNAGWT
                     NVNELIPWRTLTGRQQFYQDHAWMRDFGEGFALYRPPVDMHATAPLQGKKDNGHPEVV
                     LNFITPHQKWGIHSTYTDNLIMLTLSRGGPIVWISEIDAKKAGLVDNDWIECFNANGA
                     LTARAVVSQRVKEGMCLMYHAQEKIVNVPGSQVTGQRGGIHNSVTRTVVKPTHMIGGY
                     AQLSYGLNYYGTTGANRDSFVIVRKLAKVDWMDRVQRKAREEELDTKLVEAR"
     gene            complement(14613..15878)
                     /locus_tag="VFK00_06225"
     CDS             complement(14613..15878)
                     /locus_tag="VFK00_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HET7592585.1"
                     /translation="MGKAWRVLCASTFAFVVCFAVWMVFGVIGIPIRRELGLNATEFG
                     LLTATPVLSGAALRLPLGIWTDRFGGRAVMTTLLLACAVPVWLVSYASQFWQFLLIGL
                     ILGAVGASFAVGTPYVARFFPAARRGFAMGVFGAGTSGAALNLFVAPWIVDHHGWQMV
                     PRVYAIALLATAVLFRLLSAPDPGVDATRKSHGFAVQLRVLRDPRVWKYCQYYSLTFG
                     GFTALSLWMPQYFIGEYGVAIAAAAALAASFSLPAGALRALGGALSDRFGAHATTWWV
                     LWLTWIVLFLLSYPQTELIVHTIRGPAQFGIGLDLWMFTPLLFALGLALAFGMASTFK
                     YLGDDFADNLGVVTGIVGLAGGLAGFLLPILWGALLDLLGIRSSAFMLLYGIVWVSLI
                     LIYFTEVRRVDYPGSHDLRAPPQSRNALH"
     gene            complement(16232..16534)
                     /locus_tag="VFK00_06230"
     CDS             complement(16232..16534)
                     /locus_tag="VFK00_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592586.1"
                     /translation="MRAIRGNVLALEHYGHGHITKALAAQLADQITAHVNTIIANCKL
                     PPDADAALHGIIGSLLQNASALKADPGRSDTVAGMHKALDQYGRTFDDPAFSQGED"
     gene            complement(16775..17365)
                     /locus_tag="VFK00_06235"
     CDS             complement(16775..17365)
                     /locus_tag="VFK00_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7592587.1"
                     /translation="MDTIIQTAAGRDAGRPEPAPTVRVWDPLVRLLHWSLAACVFGAF
                     LVEEGDTAHRVLGYIAMGLVALRVLWGFVGTPHARFRDWVRGPRAVRAYLHERLTGMS
                     RRRLGHNPAAGMVMLALLAGVVLVGVTGWMQTLDAFWGAQWLENLHEVLAWGLLGLIG
                     LHVLAALVESFHYRENLIAAMIHGRKRPLDGGEPPP"
     gene            complement(17379..17651)
                     /locus_tag="VFK00_06240"
     CDS             complement(17379..17651)
                     /locus_tag="VFK00_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7592588.1"
                     /translation="MKKIATLVLCGSAALFSLGAYAAPVCTKAAQSQWMPQQALKDRL
                     VKQGYTIDKFLVSGTCYEIYGKDKAGKLVEIYFDPTDGHIVKQRIK"
     gene            17921..18241
                     /locus_tag="VFK00_06245"
     CDS             17921..18241
                     /locus_tag="VFK00_06245"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592589.1"
                     /translation="MKSETAPATGNEESLKRSVEQVKDAAETLLRTTVGQTGEEWRRA
                     RHAFGGKARALRSSMSGSAKEFAEEARELGGKGQRLVQQHPWASIGIGAGLGLLAGLL
                     VRRR"
     gene            18281..20857
                     /locus_tag="VFK00_06250"
     CDS             18281..20857
                     /locus_tag="VFK00_06250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7592590.1"
                     /translation="MSAREARASAEAPTTEALDGQWRLGLSLAEARRRLIDSGPNALP
                     GGERKPLHRIVLKVLAEPMFLMLLVAGALYLALGDHAEAAFLLGSIFAIIGITLFQER
                     RTQRALEALRDLSAPRALVIRDGQPLRVPGREVVPGDLLALHEGDRIPADAILLEGEL
                     EVDESLLTGEAIAVVRAAGTDRNRVYASTVVTRGVALARVSSTGTATKVGEIGAMLQS
                     AREPVSGLQRSSRKLIRGLAAIALLLAIGYCLTNWLWNGRTFLDSLLASVALSMAILP
                     EEIPVILTVFLAMGAWRLSRHRVLTRSVPAVEALGAITVLAVDKTGTLTQNRMRITAL
                     VTAAGALEDTPSDATGPLPAATEDLAEFALLATPSDPFDPMETAILALGDRLDDSRRM
                     HRGWTLQRVYALSSQTLATTRAYAVAGEAGHCVAAKGAPETIVALCRLPQESASAILD
                     RAAALAARGLRVLGVARGVWSGTMWPERQQDFAFEFVGLLGFGDPPRPEVPAAIAECR
                     SAGVRVLMLTGDHPVTAQAIAREIGLSERADVLTGAEIAQLGDASLAERLRQVDICAR
                     LRPEQKLRLVRLLQQSGEVVAMTGDGVNDAPALKAADVGVAMGERGTDVAREAAALVL
                     LDDSFASIVRAIRQGRRIYDNVGKATAFVFAVHVPIIVLALLPALLHWPVILAPVHIV
                     LLELLIDPASSMVFEVEPGRPDLMRRPPRPRAHTPFAPGRLAYAISQGAGLAALLIAG
                     CAVLLMHGGDAAQTRAAGFAGLLVGLFLLAMAESSFGLLRHERRNPRLKWMLLGVATT
                     LAGVFGIPSLRALMGLATPALQTLVAVGAVVLATSGWLAALQLGVRTFGQRGSGNAIV
                     SE"
     gene            complement(20847..21275)
                     /locus_tag="VFK00_06255"
     CDS             complement(20847..21275)
                     /locus_tag="VFK00_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7592591.1"
                     /translation="MNILLPVDGSPSSSRAVRYVIRHWGGTPAKRRVSLILLHVDPPL
                     PERVARVLPAETRAGYHDDHSKLAARPARRALRKAGLEFEERHAVGDPAMYTVSLATK
                     ERCDLIAMGSHGRGAFLSAVLGSVVMKVLSRSRVPVLVIR"
     gene            complement(21412..21906)
                     /locus_tag="VFK00_06260"
     CDS             complement(21412..21906)
                     /locus_tag="VFK00_06260"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HET7592592.1"
                     /translation="MITSPSVEDTAAATWILVADRARARLFSINRRAGRMIEFEDFVD
                     PEARTPGHEPEHAPPPRVHDRVGEARHAIEVHTSPREKAALQFAASLGSYLKHAHGRR
                     RYREMVLIAPPRFLGALNAALGAHVGETVLLRVAKNMVRLSASEIGEALPHGLLKRCA
                     VAGG"
     gene            complement(21884..22534)
                     /locus_tag="VFK00_06265"
     CDS             complement(21884..22534)
                     /locus_tag="VFK00_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7592593.1"
                     /translation="MNDKVLRQLVIDELEYEPSIDAADIGVTAENGVVTLSGHVPDYV
                     QKMAAERAAWRVKGVKGIAQKIEVRLPSDKKWSDDEIAQRALNILAWNTLIPKDCVRV
                     RVSGGWITLSGTMNWNYQRQAAENEVRKLSGVNGVTNSITLSSAVQAGDLKRRIQDAL
                     KRHAEVEADAVHVDVRGDGTVRIEGRVDNWSEMKAVEHAVWSAPGVQRVDDYLSFR"
     gene            complement(22568..22786)
                     /locus_tag="VFK00_06270"
     CDS             complement(22568..22786)
                     /locus_tag="VFK00_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592594.1"
                     /translation="MQAILILSPILVCAFVTGVLLMWVLFVTLPRMRRAEKGAPPASR
                     PALRTTIPPRSTKSSIVDADQETTGRNV"
     gene            complement(22882..25458)
                     /gene="mgtA"
                     /locus_tag="VFK00_06275"
     CDS             complement(22882..25458)
                     /gene="mgtA"
                     /locus_tag="VFK00_06275"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HET7592595.1"
                     /translation="MNDAPRPAAASDPARVTQESLYMRLASSAAGLGEAEAAHRLAEH
                     GPNEAHAGDRAGPLQTLMELFGNPLVLMLLVAAIVSGFLGDRIGAGIIVAMVLLSVTL
                     NYVLSYRSKRAAERLREEIAPTASVCRDGAWRDVPRRTLVPGDVIRLAAGDRVPADAR
                     LLQARDLHVQQSALTGESAPAEKEVCEQPGATAGADARHLVYLGTSVVAGSATAIVIA
                     TGRDTAFGDVAARLSERPPPTEFERGLAGFARLIMRTVIGLVLLVVLAGIVFHRPLLE
                     TLLFALALAVGLTPEFMPMITTVTLARGAKRMARHGVVVKHLAAIEDFGSMTVLLSDK
                     TGTLTRNETDVFAAVDVYGDPSTRAGGLAHLNSSFQTGIRSPLDEAILRGCTPPQDES
                     RKLDEIPFDFERRRLSVVATGPGDTALLVTKGAPESVLPLCTGYAVDGVVRPLELDAQ
                     AAARRVCDAYARQGLRALAIAYREIDRRPRYAAEDEAGMVLAGFVSFADPILPDVAAA
                     VRALARDGVAVKILSGDNELVVRHVCEQVGLDTREVMTGSEVEKLAPDALGVVAERVS
                     VFARLSPAQKHRIVLALKARHAVVGFLGDGINDAPSLHAADVGISVANAVDVARDAAD
                     IVLGERDLGALHAGVIEGRKAFANVMKYLLMGTSSNFGNVLSMAAASLVLPFLPMLPT
                     QILLNNFLYDMAQVTIPGDNVDPAQLVAPRRWNVRSIRQFMLGVGPISSLYDFLTFYV
                     LFAWLHASQAEFHTGWFVESLATQTLVLFVIRTAGSPLRSHPSKPLIATVFAAVITGT
                     ALPFTPIGADLGLIPLPPVFFAFLAVATATYLLLVELVKRRFMGADTGNPAMVQGAAS
                     VA"
     gene            25625..26068
                     /locus_tag="VFK00_06280"
     CDS             25625..26068
                     /locus_tag="VFK00_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7592596.1"
                     /translation="MAAITRFNPFRSPARFETSGAFDDLFRNLGFGSSWSQPEFMSDM
                     RVDITEDDDAYYIDAEIPGVNKDDIDVSVEGNRVAINAEAKREKQKKDEKELMVERSW
                     GRAYRAFALPGDIDGNRTEARYDKGVLTLMLPKNANGNARRIAVS"
     gene            complement(26199..26558)
                     /locus_tag="VFK00_06285"
     CDS             complement(26199..26558)
                     /locus_tag="VFK00_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET7592597.1"
                     /translation="MKIPVRVTFQGMDASEALRAKIEARAERLQRYADDIQTCDVVVR
                     ACERRHRHGNRYNVHARVAMRDREIEAGRTPGADSDHEDPYEAAAHTFDVLRRRIEDQ
                     VRRRRGDVKSHVRATVD"
     gene            26757..27575
                     /locus_tag="VFK00_06290"
     CDS             26757..27575
                     /locus_tag="VFK00_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7592598.1"
                     /translation="MKDILVYVRDFEERTPAAHFGVRLAATFGASVTGVYTCAQPVYL
                     APAYEPELMARAVEDARELAKDAVQSKRSFLDWATSLGVPQTEWLVVEGDARDALAEA
                     ATRHDLLVLDHAEEGRGSSWDIPGLVLKTDIPCVVLPHHGVHYTQFERIAIGWNGSPE
                     AMRAVHAALPFLKGRQALLMRGEERDKYPGLEWDPPFNIMDYLKRHGVTAEQRTIRAK
                     HDDVGEVLLDEAMKFRADMLVMGAYGRSRFSEWMLGGATRHVLTWADIPVLMRH"
     gene            27608..>27915
                     /locus_tag="VFK00_06295"
     CDS             27608..>27915
                     /locus_tag="VFK00_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7592599.1"
                     /translation="MVLESVGTRLVLKELANPALHPGEVLVRVLACGICRTDLHVVDG
                     ELPRVRPPVVPGHEVVGIVEQVGEGVEAVAPGAHVGIPWLGGTCGECAYCRAGRENLC
                     "
BASE COUNT         4700 a         8994 c         9471 g         4750 t
ORIGIN      
        1 cgcatcggca tgatcgaacc ggcgcggatg ctcggcatgc tgtcggacga tgccgcgctc
       61 gggccgatcg cgcgcgaggt cggtgacgcg acccgccgga tgatcgacga ggcgaagtga
      121 tgcggcagcg ccccatccac ctgcggcggt acgccgtgcc cgtagtgctg gctgcgttgc
      181 tggcggcctg cggcgcgtcg cggcaccctc cacagtcgcc gcagccgtcg ctggcgacga
      241 tcgccgtgca gccgcggcag gccgcgcgcg aacaaacgtg ggacggcgtg gtcgaggcgg
      301 tggatgccac gacgattgcg gcgcagacca acgcgcgcgt gctcgaactg ccattggacg
      361 tcggtgaccg cgtccgcaag ggcgacgtgc tggtgcgctt cagcgacgtc gagcagcaat
      421 ccggacggcg cgccgcctcc gccaacgtcg cggcggcgcg tgccgaattg cgcgacgccg
      481 aggcgaactg gaaacgcacc gatgcgattt tccagcgcgg cttgatcgcg cgcgcgcaac
      541 tggatgccgc ggtcgcgcgc cgcgactccg cacgcgccgc gctggccgcc gcggaagccg
      601 cgctgcgcag tgccggccag caggccgact acaccgtgtt gcgggcgccg ttcgacggcg
      661 tgattaccca gcgtttcgtg cacgtgggcg aagcggtgca atcggggccg ccgacgccgc
      721 aaccgctgat cgcgctggcg gcgctggacg ccctgcgggt ggacgtggtg gtgccgcaag
      781 gcgccgtcgc tgccatccgc gcttcgccgc gcgccagcgt ggtgctggat gacggcaaac
      841 gcgtgcaggc cgccagggtc accgtgttcc cgtacgccga tccgggcacg cactcgttcc
      901 gggtgagggt ggaattgccg gcgggcggca gcggcttgta cccgggcatg accgtcaagg
      961 cggcatttgc cgtcggccag gcgacacggc tgctggtgcc ggtcggtgcg ctggtccggc
     1021 gcggtgaatt gagcgcggtg tacgtggtcg gcgccgatca ttcggtgggc cttcgccagt
     1081 tgcggctggg ccaccgcatc ggcgacgagg tcgaagtgct ggccgggctt gccccgggcg
     1141 accacgtcgc gcgcgacccc gaagccgccg cgctgtacct caccaggcag cacggtggga
     1201 acgcgtcgcc gtgaatgcac cacgtctggg gttcgccggg cgcgtcgcac gcgtattcca
     1261 ggatcatccg ctgacgccga tcctggcatt ggcggcgctg ctgatcgggt tcaccgcgct
     1321 gctgatcacg ccgcgcgagg aagagcctca gatcgacgtc accatggcca acgtgatcgt
     1381 gccgttcccg ggcgcggacg tgcatgacgt cgaaaacctg gtggcgtcgc cgctggaaca
     1441 gaagctggcc ggcatcgacg gcatcaagca cgtgtattcg gccagccgtc cgggcgtggc
     1501 gctgctgacc gtcgagttca aggtcggcgt gccgcgccag accgcgctgg tgcgcctgta
     1561 caaccaggtg ttccagaacc gcgacctgtg gccgccggcg ctgggcgtcg gccagccgat
     1621 ggtgcgcgcg atgggcatcg acgacgtgcc ggtgctggcg ttgacgcttt ggatcgacga
     1681 tccggcgcgc ggaccgatcg atctcgccga ggtcgcgcac acgctcgaaa ccgacctcaa
     1741 gcacatcccc ggcacccgcg acgtttacac gctgggtgcg cccgatcgcg cggtggtggt
     1801 gcaggtcgat ccggcgaaac tcgcggcgta cggcatggcc gtcgatgacc tcgcacagtc
     1861 gctcagggcc gccaacgtgg tcacgcaggc gggcgatcgc gtcgcggggg accacgacgt
     1921 tccggtgacg gccggcacct tcctcggagg cgccgacgac atcgcgcaac tggtgatcgg
     1981 actgaagggc ggccagccgg tgttcctgtc cgacgtcgcc acgatccgcc ggggcgccga
     2041 cctgccggac cggtacgtct ggttcggcac gccgccgggc aagggcggcc ccgcgaccgg
     2101 cgcggcgccg gcggtgacga tggtcgtcac caagttgtcc ggcaccaacg ccgccgacat
     2161 cacgcaggcg atccggcatc gcctcgacgc actcgaaggc gtggtgatcc cggccggcgt
     2221 gcatgtgatc gtgacccgcg actacggcga aacctccacc gcgaaagccg acctgctgat
     2281 gcacaagctg ctgttcgcca cgctgtcggt ggtgctgttg gtgctgctga cgctgggctg
     2341 gcgcgaagcg atcgtggtgg gttcggcggt ggtcatcact ttgatgctga cgctgttcgc
     2401 gtcgtgggcg atgggattca cgatcaatcg cgtgtcgctg ttcgcgctga tcttctcgat
     2461 cggcatcctg gtggacgatg cgatcgtggt ggtggagaac atccaccggc acatggcgct
     2521 gggagggcgc tcgttgcgcg aggcgattcc ggaagccgtg agcgaagtcg gcgggccgac
     2581 catcctcgcg accttcaccg tgatcgcggc gctgctgccg atggcattcg tgtcgggatt
     2641 gatgggcccg tacatgcggc cgatcccgat caacgcctcg gccggcatgc tgctgtcgct
     2701 ggcggtcgcg ctggtggtga ccccgtggct ggcattgaag ctggtgcgac ggcacgcatc
     2761 ggctcccgca cacgacgacg gtccgcgcga gacgcgcctg ttgcgcctgt tccggcgcgt
     2821 gatgacgccg ttcctcggcg gcggccgcgc gccgagcagg cggcgcctgc tgttcgcgtc
     2881 gatgggcgtg ttggtgctgg cggcggtggg gttggtggtg gtcaaggcgg tggtgctgaa
     2941 gatgctgccg ttcgacaaca agtcggaatt ccaggtggtc gtcgacatgc cggaaggctc
     3001 gacgctcgag gccaccaacg cgctgcttgg ccagctcgcg cacgcgctgg accgcgtccc
     3061 tgaagtcatg gactaccaag gttacgccgg caccgccgcg ccgatcacct tcaacggatt
     3121 ggtgcgccag tactacctgc gctccggacc catcgacggc gacctgcagg tgaacctcgt
     3181 cgacaagtcc gaccgcgatc gcaagagcca cgagatcgcg ctggcggtgc ggcccatgct
     3241 ggcgaagatc ggcgatcgct tcggggcgtc gatcaaagtc gtggaagtgc caccgggtcc
     3301 gccggtgctg gcgccgatcg tggccgaaat ctacgggccc gattacgcca ccttgcgcaa
     3361 gctcgcgcgc gggctggaga aggacttccg atccgacccg gatatcgtcg acatcgatac
     3421 cagcgtcgag gccgattcac cgcgcgcggt ggtggtggtc gatcgcgcac gcgctgcgcg
     3481 gctcggcctc gaccaggcgc agatcgctca ggcgctgcgc tttgcgctgt ccggcgacga
     3541 cgcgacgtac ctgatggatg gccgcgcgcg ctacgcggta ccggtgcggc tgcgcctgcc
     3601 gccgggcaac caggccgcgc tcgaacagtt gttgtcgctg cgggtacgcg cgaaaagcgg
     3661 cgcgctggtg ccgttgtcgg cggtggccag cgtgcgcgag cccggctggg aaaaggcgat
     3721 ctaccacaag gatctcttgc cctacaccta cgtgacaggc gacggcgccg gccgcacaga
     3781 cagcccgctg tacgggatgt tcgcgatggt tgcgaaactg cgccacggcg aagcggacgg
     3841 ccatcagctg tcgcaaaact tcgtccacca accggtcgac cccgccggct acgccgtgaa
     3901 atgggacggc gaatggcaga tcacctacga gactttccgt gacatgggca tcgcctacgc
     3961 ggccggactg gtgttgatct acctgctggt ggtcggccac ttcaggcgct acatggtgcc
     4021 gctgatcatc atggcgccga tcccgctgac ggtgatcggc gtgatgccgg gccacgcgtt
     4081 gctgggcgcg cagttcacgg ccacgtcgat gatcggcatg atcgcgctgg ccggcatcat
     4141 cgtgcgcaac tcgatcctgc tgatcgactt catcgacctg gaactcgcca agggtcgtcc
     4201 gcttgccgaa gccgtgatcg aagccgcggc ggtgcgcgcc aagccgatcg cgctgaccgc
     4261 gctggcggcg atgatcggtg gcttcttcat cctcgacgat ccgatcttcc agggcctcgc
     4321 ggtgtcgctg atcttcggca tcctggtgtc gacggtgctg accctgctgg tgatcccgct
     4381 gctgtactac gcatggctcg cgcgcgccgg cggtgggcat ccatgagatg gacgtggtgc
     4441 ccggcgtgca gcgcggttca cgtcaattcg aggtgatagg cctccacgga cggatcgtgc
     4501 aggtccaggt ggcgcttgaa gcccaggcgc gacgcgagac ggtggcttcc gccggattgc
     4561 gccggggcga aggcgcgcaa gtgccggatc ccgcgttgac gtgccgcgtc gatcagcacg
     4621 tgcatcagcg atgatccgac cccgcgcttt tgccattcgt cgctgaccgt caccgaacag
     4681 tcgcaactgt cgcccttcgg actgacatgg aaatgcgcgg cgccgatctg gcgttcgcgg
     4741 ccgccgtcgg ataccacggc gatgaaaccc gccgcgcacc tcgggtcgag atcggtcagt
     4801 ccgcgcgcca cgtcgggtcc gggttcccgg atcaggccga ggaaacgcag gccgcgcatc
     4861 tcggacgaca ggccgttgag gaactccagc tccaggccgg cgtcctgctt gcggatcggc
     4921 cggatatgca ccagcgtgcc gtcgagcagg cgctggttcc agcccatgcc ggggggcgaa
     4981 gcggcccgtg gactatggtc gatgcggtcg cgcgcgtcgg gtcggggcat cacgttcacc
     5041 ttgccgggtt ccgggagaag ccccaccgta gcgtcgcgcc tgcaccgctt gttgatcgtc
     5101 gtcaacaact tgcggtcctg ccgcgcgtca cgctcggtgg tgccgggatc cgccgcattc
     5161 ttgacggcga tcaacggatt tgccgcggcg atggcgacaa tgaccgtgtg tcggcatggt
     5221 gaagcaggca tggaattcca catccggatc cggggcgcga gtccgaacct cgacgcgatc
     5281 aacgcggcgc tcctcgatgt cgatccgacg gcggtggccg acatcgagcc tggcggtgcg
     5341 agcctcaggg tgtcggttgc gctgggttct cccgaactga tcgcactgct cacgcaggcc
     5401 ggctacccgg tcgactggaa acagctcgaa gaagtgcctt cggtgtgttg cggcgcgtgc
     5461 ggtggctgat gcgaaaacac gggcgcgcgg accgtcggga atgcaggcag gcaaccacac
     5521 gggagggacg gcatgggtga cgagcagagt ttcgaggtga gcctggagca ggtcgaggac
     5581 ttcgagttcc gcgtgcactt cgatgggacc gcgctcgagg atttgtccac cgacgagcct
     5641 ccgccgcagg ggcgcaatgc cggccccaat cctgcgcgga tgttgctggt tgccgtggcg
     5701 aactgcctgg ccgcgagcct gctgttctcg ctgcgcaagt tccgcaacgc gcccggtccg
     5761 ctgcgcgcga aggcgcgggg ccgcctcgcg cgcaatgcac aggggcgctg gcgcgtggcc
     5821 ggcatggaag tggacctcca gctcgcggac ccggcagcgt cgctgcagca cgtcgagcgc
     5881 gcgctcgcac agttcgagga tttctgcatc gtcaccgaaa gcgtgcgcgc gggcatcccg
     5941 gtaacggtac gcgtgcatga tggcgaaggc agggaagtgc acgccacggg aaatgccgct
     6001 tgacggatcc tggcaccggg cggcgccccg cacgccgtat ttcccggtac tcgccggcgc
     6061 ttgagggtgt tggtcaacgt gccgtcactt cagcctcgcg cgtatgcgcg ccagtcgcgg
     6121 cgcagcgcgc gcaattgtcc ggcgaagagg agcaaccagg ccgcgcaacc ggccgcgaac
     6181 atgaagtgcg gcagcagccc gaggaagggc aggtccatcg cgtgcatcat tcgcagcgtc
     6241 gcggtggcgt acatgccgag cgggaacacc gcgccccagt acatcgggtc gtaggtgaac
     6301 gggaaacgct tgatgccgtg gcgccagatc gccagcaccg ccagcatcgg gatccaccag
     6361 gtgccggtcg cccagtagaa caccgtgaag cctttcagga acggcagcat cgagccgagg
     6421 aacggcgcgt gcggcgtgtt ctcgatcagc agcgatccgg ccaacgtgga aatcgccatc
     6481 gcgcccatgt tgatccagta cggcggcgac aggtcggcgg gcgaaaacgc gaagaaggta
     6541 tagcggtaga agatcagcga catcatccag atgtacagca tgccgcccca cagccacatc
     6601 gacagcgcga agaagttgag ttccatccgc agcggctgcg cgacctcggg cgcgagctgc
     6661 gcgcccagca ccgccaggga ttgcgtggcg acgaccgcca gcagccagcc gccgttgatg
     6721 ccgcggtcga gcgccggctt ctgctgcttg acggtcagca cggtgaagat ggtgtaggtc
     6781 aatcccaccc acgcgagcag cgccacgacg ccgaatgcgg ccgcgatgcg cgcgctgccg
     6841 ccgatcagca acgcctggct gccgaggatc gagcaggcgg cgacgaaggt gaagaaaccc
     6901 ggcgcctgca ggtggtcgaa caggtcgccg agcgtgcgcc gcctgtgcca cgcgaggcgc
     6961 gccaccgcca ggatgcagat gatcgcgaac aacaccgcgt tcagcgcgaa cagtgccttc
     7021 gcaagcagcg gcaggccaag caggtgcgag gccagcgaca ggatgccggt cgccatcacc
     7081 atgctgaagt agcccggcga catcgtttcg atggacgcgc gcaggtgcgc gatgaagccg
     7141 tgcggcgctg gtgcgaagct gttgggcgcg ggcccgctca tcgcctcggc gtcgccaatg
     7201 gcagggtcac tgcaccagcg gcgaggcggc agcgtccagc tcgacgcctt cgatgtcggc
     7261 gttgccggcc agcacgcgca cgtactgcga cagcgcgcgc gcctcgctgc ggcggcgcag
     7321 ttccgcggcc acgtcgtcgc gcacgatgtc atagggaagt tgcctcccct cgatgcgccg
     7381 gtccaccgag atgatgtgga agccgtgtcg ggtggtcacg agcttcggca ggatgccgat
     7441 gccggcgtcg tcgccgaaaa tggccttctc gaattccggc agcgtttcgc cgcgttgcag
     7501 ttggccgagg ttgccgccgt tggcgcccga cggacagttg gaatgttcgc gcgcgcgttc
     7561 gccgaataaa gcagggttgg accggatttc gtgcagcatc gcttcggcga ctgcgcggat
     7621 cgccggcacg ggcgcacccg gcgtgacctg gaacaggatg tgccgcgcgc acaccaggtc
     7681 gccgctgcgg aagcgctgct ggttggattc gtaccagcgc cggcattctt cctcgtgcgg
     7741 ttccggcacc ggcacttcct cggccagcgc gcgctcgatc gcacgctcgg tgtcggcctc
     7801 gctggtgtcg tcggtgatga agcccagttc gcttgcacgt tggcgcaaca gttcgtgaac
     7861 cgccgcaagt tgcggggtcg gccaatcggc cgggtcgacc gtaacgccgt tgatggtgat
     7921 gctcatgtgt tcaccgaacc gccgcgcttg cgcacgagtt ggtacggccg taccagatac
     7981 gcgagcgagc cgaccccgct ccagatgtgc accatgcgcg tgaagggcga gaccagaaag
     8041 atcgtgaagc cgagcaggac atgcatctgg tagggccagg cgaggccctg cattattccg
     8101 gcaatgtccc tgtggaaata caccacgccc tgcacgtaac tgacgatgcg ctcgaattcg
     8161 ccgctgccca tgtgctgcgc ggaaatcggc accgtcgcca gccccagagc caattgcagc
     8221 cacaggatga acacgatggc aatgtcccat ttgcggctgt tgccacggat gcgccggtag
     8281 tagagccggc gatggatcag gatactgacg ccgacgatgg cgatcaggcc cgcgatgccg
     8341 cccaccacca tcgccagcag ttggtgctgc ttcgggctca tcagccacaa caccagcgat
     8401 tcgggcatga ggaagccgac gaaatgcccg aggatcacga ccagcacgcc atagtggaac
     8461 aggttggaac ccagccggag ctcgcgcttg tacagggtct gcgaggaatc gctcttccac
     8521 gagtacggct cgcggtcgaa gcggatcagg ctaccgagca gcagcaccgc caggcaaatg
     8581 tagggataga tgccgaacag gaagaagttc agtggtgcgg gcagggtcat ggtggcatct
     8641 ccagtcggat catgatgtgg ggtgttcggg cgtgacggcc ggattcggtt cgggtggcgc
     8701 gaacgccggt ctttcttccc acgcctggtc gaggtcttcg gccggcggca ccgccgcgtg
     8761 cgcatcgaga ccctgttcgc cgcccagcgc cagcagcgcg gccatcactg cggcgtagcg
     8821 gctgtgtttc ttcagcaggg tgttgccgag ctggcgcagg atgtgcgcga tctcgccgat
     8881 ggtgtcgcgg acttcgccgg catcgcggca actcaggtat tccagcagca gcggcagatg
     8941 gtcgggcaac tcatccgcga cgggctccat tccggcgtcg aggtagcgct gcttgagttc
     9001 gagcatcgcc gggccgcgct ggcggccgtc gccgtggaca tgctcgaaca ggttcagcga
     9061 cgtgcgccgg ccgcggtcga acaggtcgac gtagcggcct tcggcttcca gcgggtcgct
     9121 cgcggcgatc tcgtcgacca gcacgaccaa ttgctcgcga agcgaagcgt cgacggcgcg
     9181 cgcgtcgcgg atcgccccgg cgatttcggg caacgccgcg cgcaactcgg tgtcggggta
     9241 ggcgagcagg gcagacagcg cgcgcagcag cagggatgga tcgcgggtcc tcatgcgtgg
     9301 tcgtcgatgt tgcggatcgg aatgaccttg cgccggccca tctttccgcc gaacaggccg
     9361 gccttggcgt cgatgccgtc gcacccattg ccgaaggtga aaccgcagcc gccgcgcaac
     9421 tggtaggcgt tctcggcttc ctcgcggtgc gcggtcggga tcacgaaacg atcctcgtag
     9481 ttggcgatcg caaggtatcg gtgcatgtcg ttggcggtgg cctcgtcgag cccgacgccg
     9541 gacagcaatg ccgcatcggg agcattgccg agctgcttgc cgcggaacca cgcgcgcatc
     9601 gccagcatgc gttccagcgc actgacgatc ggcggctcgt tgccggcggt gaggaggttg
     9661 gcgaggtaac gcaccgggat gcgcagcgag ccgacgtcgg gcaagccgtt cgcggcgcta
     9721 tcgatgttgc cggaattggc gtgcgcgctg atcggcgaca gcggcggcac gtaccagacc
     9781 atcggcagcg tgcggtattc cgggtgcagc ggaaacgcga tcttccattc caccgccatt
     9841 ttgtacgcgg gcgacttctg cgccgattcg atccatgcgg cgggaatgcc gtccgccaac
     9901 gcctggcgct ggatttccgg atcagacgga tcgaggaaga cgtcgagctg cgcctggtac
     9961 aggtccatcg ggttctcggt ggacgcggcc tgttcgatgc ggtcggcgtc atacagcagt
    10021 acgccgaggt agcggatgcg ccccacgcag gtttccgaac acaccgtcgg caggccggcc
    10081 tcgatgcgcg ggtagcagaa ggtgcacttc tcggccttgc cactgaccca gttgtagtag
    10141 atcttcttgt acgggcagcc ggacacgcac atgcgccagc cgcgacactt gtcctggtcg
    10201 atcaggacga tgccatcttc ctcgcgcttg tagatcgaac cggacgggca ggacgcgacg
    10261 caggtcgggt tgaggcagtg ctcgcacagg cgcggcaggt acatcatgaa agtgttttcg
    10321 aactggccgt agatgtcctt ctgcacggct tcgaagttgt aatccttgct gcgcttgtcg
    10381 aactcgccgc cgaggatttc ctcccagttc ggaccccact cgaccttgtc catggtgcgc
    10441 ccggacacca gcgagatcgg ccgcgccacc ggcgcggtcg gcaattccgg cgcggtctgc
    10501 aggttttcgt agtcgaaggt gaacggctcg tagtaatcgt cgatctcggg caggtgcggg
    10561 ttggcgaaga tcttcgccag caccgcccag cggccgccct gcttgggcac cagccggcca
    10621 ttggacttgc gttcccagcc gcctttccag cgttcctggt tttcccagtc cttggggtag
    10681 ccgatgccgg gcttggtttc gacgttgttg aaccacgcat attccatgcc cttgcgcgag
    10741 gtccacacgt tcttgcaggt gaccgaacag gtgtggcaac caatgcactt gtcgaggttc
    10801 agcaccatcg ctatctgtgc gcggatcttc atcagcgggc ctccaccagt ttggtatcga
    10861 gttcttcttc gcgggccttg cgttgcacgc ggtccatcca gtccaccttg gcgagcttgc
    10921 gcacgatcac gaacgagtcg cggttggcgc cggtcgtgcc gtagtagttg agtccgtacg
    10981 acagttgtgc gtagccgccg atcatgtggg tgggtttgac cacggtgcgg gtgaccgagt
    11041 tgtggatgcc gccgcgctgg ccggtcacct gcgaaccggg cacgttcacg atcttttcct
    11101 gcgcgtggta catcaggcac atgccttcct ttacccgctg gctgaccacc gcgcgcgcgg
    11161 tcaacgcgcc gttggcgttg aagcattcga tccagtcgtt gtcgaccagg cccgccttct
    11221 tcgcgtcgat ttccgaaatc cacacgatcg ggccgccgcg agacagcgtc agcatgatga
    11281 ggttgtcggt gtaggtgctg tggatgcccc acttctggtg cggggtgatg aagttcagta
    11341 ccacctcggg gtggccgttg tccttcttgc cttgaagagg cgcggttgcg tgcatgtcga
    11401 cgggtgggcg gtacagcgcg aagccttcgc cgaaatcacg catccacgcg tggtcctggt
    11461 agaactgctg gcggccggtg agggtgcgcc agggaatcag ttcgttgacg ttggtccagc
    11521 cggcgttgta gctgacgtgc tcgctctcga tgcccgacca ggtcggcgag gagatgatct
    11581 tgcgcggttg cgcctggatg tcgcggtagc ggatcttctc gtcctcgcgg gcgcgtgcga
    11641 ggtgggtgtg gtcgcggccg gtgatcttcg acagcgcggc ccatgccttc accgccacct
    11701 cgccgttggt ttccggcgcg aggtacatga tggtctcggc ggcgtcgatg tcgctgaaga
    11761 tgcgcggccg gcccttggcc ggaccgtcgg ccacggtgta gttgagcgct gcgaggcctt
    11821 tcacttcatg ctcggtgttc caggcgatgc ccttgccgcc attgcccagc ttgtccatca
    11881 gcggacccag cgaggtgtag cgcgcgtagg tcgccggata gtcgcgctcg accgtgacga
    11941 tgttgggcgc ggtgacgccg ggaaccagtt cgcactcgcc cttcttccag tcgcgaactt
    12001 cgaatggctg cgcgatctcg gccggagtgt cgtgctggat cggtaccagc accacgtcct
    12061 tttcaatgcc cagcacgcct tcggaaagct cggagaattt cttcgcgacc gccttgaaga
    12121 tttcccagtc gctcttcgat tcgaacaccg gatcgaccgc cgccgacagc gggtggatga
    12181 acgggtgcat gtcggtggtg ttgaggtcgt tcttctcgta ccacgacgcg gtcggcagca
    12241 ccacgtcgga atacaggcac gaggtggaca tccggaagtc cagcgtcacc acgaggtcga
    12301 gcttgccctc cggcgccggg tcgtgccatg ccacttcctc cggcttcacg ccaccgtcct
    12361 cgccgagatc cttgccctgc aacccgcagc gtgtgcccag gaagtgcttc aggaaatact
    12421 cgtggccctt gccggacgag ccgaacaggt tggagcgcca gatgaacagg ttgcgcggga
    12481 agttcaccgg attgtccggg tcctcgcagg ccatcctgat gtcgcccgac ttcagcgccg
    12541 cggcgacgta atccttcgcg tccttgccgg cggccgcggc gtcgcgcgcc acgtccagcg
    12601 ggttgcgatc caattgtggc gccgacggca tccagcccat ccgttcggcg cgcacgttgc
    12661 agtcgatcag ggtacggccg tagcggctgg catcggccag cggcgacagt tgctcggcgg
    12721 tcttcagcgt ctcgtagcgc cactggtcgg agtgcgcgta gaagaacgag gtgccgcaca
    12781 tgtgccgcgg cgggcgcacc cagtccagtg cgaaggccag cgcggtccag ccggtctgcg
    12841 ggcgcagttt ttcctggccg acgtaatgcg accagccgcc gccggacacg cccacggtcc
    12901 cgcacagcat caggaagttg atgatgctgc ggtagttcat gtcctggtgg taccagtggt
    12961 tcatgccggc gccgatgatc accatcgaac gaccgcgggt tttctcggcg ttgtgggcga
    13021 agccgcgcgc cacctcgatt acatgcgcgg ccggcgtgcc ggtgatggct tcctgccacg
    13081 ccggcgtgta ggcgacgttg tcgtcgtagt tttttgcgca ttcgccgccg aggccgcggt
    13141 cgaggccgta atgcgcgcac agcaggtcga aggcggtggc caccatggtt tcgccgttct
    13201 tggtggcgag cttgcgcacc ggcacgcggc ggatcagcaa gccgtcggca ccctgcgtgt
    13261 tggcggtgaa gtgcggattg gcgacgccac cgaaatacgg gaacgccacg tcggcgacgt
    13321 cgtcgcgccg gtcgatctgc gtcaactgca attcgatgtc gcgaccgtcg ccggattttt
    13381 cctcgaggtt ccacttgccg tggtcgaggc tgtcgccttg cggccagcgg aagccgatgg
    13441 aaccggtcgg caccaccggc tggccgtcct tgtcgaagcc gaccgtcttc cagtcggcat
    13501 tgccgggcga acccagcgcg ccctcgaaat cgctggcgcg caggtaacga tcgggcaccc
    13561 agcgcgcacc atcgcggcgc agcgtcacca gcaagggcag gtcggtgtac ttgcggcaat
    13621 agtcctggaa gtagggcacc tgccggtcga tgaagaattc cttcaggatc acgtggccca
    13681 tcgccatcgc ggcggcggcg tcagtgccct gtttcggatg caaccacagg tcggaaagtt
    13741 tcgcgacttc cgaatagtcc ggcgtcaccg ccacgatttg cgtgccgttg tagcgcgctt
    13801 cgacgaagaa atgcgcatcg ggcgtgcgcg tctgcggcac gttcgaaccc cacgcgatga
    13861 tgtagccgga gttgtaccag tcggccgatt ccggcacgtc cgtctgttcg ccccaggtct
    13921 gcgggctgga cggcggcagg tcgcagtacc agtcgtagaa cgacagcagc gagccgccga
    13981 tcaggttgag gtaacgcgaa cccgaggcga atgagaccat cgacatcgcc gggatcggcg
    14041 agaagccgac cacgcgatcc ggaccgtggt gcttgatggt gtacacgttg gccgcggcga
    14101 cgatttccag cacctcgtcc cacttcgcgc gcacgaagcc gcccaggccg cgcgcggcct
    14161 tgtaatccct cgcgtcgggg tcggccatca gcgccgccca cgcctggacc ggttgcagcg
    14221 acttgcgcgc ctggcgccag cgcttcagca ggcgtccccg caccatcggg tacttcaggc
    14281 ggttggcgct gtacaggtac cacgaatacg acgccccgcg cgaacagcca cgcggttcgt
    14341 ggttcggcat gtccgggcgc gtgcgcgggt aatccgtctg ctgggtttcc caggtgacca
    14401 cgccctgctt gacgtggatc ttccagctgc agccgccagt gcagttcacg ccatgggtcg
    14461 aacgcaccac cttgtcgtgc gaccagcggt tgcggtagcc gtcttcccac tggcgtgctt
    14521 cggtgcgcag ttcgcccaat ccgcccgaga agggttcgcg cggccgcttg aaataacgga
    14581 ggcgttccag gaattgaccc atggcgaagt cctcaatgca gtgcgtttcg ggattgcggc
    14641 ggcgcgcgca gatcgtggct gccgggatag tccacgcgcc tgacttccgt gaaatagatc
    14701 aggatcagcg acacccacac gatgccatac agcagcatga atgcgctgga acggatgccc
    14761 agcaggtcca gcagcgcgcc ccacaggatc ggcagcagga agcccgcgag gccaccggcg
    14821 agacccacga tgccggtgac gacgcccagg ttgtcggcga aatcgtcgcc caggtacttg
    14881 aaagtcgaag ccatgccgaa cgccagagcc agccccagcg cgaacagcag cggcgtgaac
    14941 atccacaggt ccaggccgat gccgaattgc gccgggccgc ggatcgtgtg cacgatcagc
    15001 tcggtttgcg gataggaaag caggaacagc acgatccacg ttagccacaa cacccaccac
    15061 gtggttgcgt gcgcgccgaa gcggtcggac agcgcgccgc caagcgcgcg cagtgcgccg
    15121 gcgggaagcg aaaaacttgc ggccagtgcc gcggctgccg cgatcgcgac cccgtattca
    15181 ccgatgaaat actgcggcat ccatagcgac agcgcggtga aaccgccgaa ggtcagcgag
    15241 taatactggc aatacttcca tacgcgcgga tcgcgcaata cgcgcagttg cacggcgaag
    15301 ccgtgtgact tgcgcgtggc gtcgacaccc ggatcaggcg ccgacagcag tcggaagagc
    15361 accgcggtag cgagcagcgc gatcgcatac acgcggggca ccatctgcca gccatggtgg
    15421 tcgacgatcc acggcgcgac gaacaggttg agcgctgcgc cgctggtgcc ggcaccgaac
    15481 acgcccatcg cgaacccgcg tcgggccgca ggaaagaaac gcgcaacata gggcgtgccc
    15541 accgcgaacg aggcgcccac cgcgccgagg atcaggccga tcaggaggaa ttgccaaaat
    15601 tggctggcgt acgacaccag ccacactgga accgcgcacg ccagcagcaa cgtcgtcatc
    15661 accgcgcggc cgccgaagcg gtcggtccag atgccgagcg gcaggcgcaa cgcggcaccg
    15721 ctcaggacgg gcgtcgcagt gagcaggccg aattcggtgg cgttgaggcc cagctccctc
    15781 cggatgggga tgccgatgac cccgaacacc atccagacgg caaaacacac cacgaatgcg
    15841 aacgtgctcg cgcacaacac ccgccatgcc ttgcccatgc tgccgtcctc acccagatga
    15901 tagcgacggg gcacatgcat catcggcgga gccgagccgc acgaatgatg cgagttcccc
    15961 cagcatagtc acacaggtct gcagatgcca gccatgggta cccgggatcc tgcgtcttga
    16021 tcgaggtcga catgtgcgcg tattgcggcg atgtgcgcgc aacgaagcac ggcgtgcgcg
    16081 aagcagattg cgccccggat cgcgacaagc ttcccatggt catgttggtc gttcgggccg
    16141 cgggagcacg gtccccagct ccgcagcggc ctcgcaggtc ccacacacag tcgcacgagg
    16201 accggcgaca tacccacagc gtggccgaga ctcaatcctc gccttgggaa aacgccggat
    16261 cgtcgaatgt acggccgtac tgatctagtg ccttgtgcat ccctgcaaca gtgtcgctgc
    16321 gtcccgggtc ggccttcaat gcgctggcat tctgcagcag cgatccgatg atgccatgca
    16381 gcgcggcgtc tgcgtccggc ggcagcttgc agttggcgat gatcgtgttg acgtgggcag
    16441 tgatctggtc ggcgagttgc gccgcaagcg ccttggtgat gtgaccgtgg ccgtagtgct
    16501 cgagggcgag gacgtttccc cgtatggcgc gcatttgttc ccgcaacgtt gcgtcggtgg
    16561 ggaagcgctg cgagggtact gcgacagggg ccgccgtgga tgcgtgggag acgtgttgct
    16621 gcgccagggc gagggatgcg ggtagaccgg tcagcaggaa aaaaccgagt gcaacgccaa
    16681 agggacgcat ggagtatttc cttcgaatgg tgggtgtcct tgaagaaccg gatcggcgcg
    16741 ccggtcgcgt gtctgaaagg ctcgcgtagg acgatcaggg tggcggctcg ccgccatcca
    16801 gaggacgctt gcggccatgg atcatcgccg cgatcaggtt ttcgcggtaa tggaaactct
    16861 cgacgagggc ggcgagcaca tggaggccga tcaacccgag cagaccccac gccaatacct
    16921 catgcaggtt ttccagccat tgcgcgcccc agaacgcatc cagcgtctgc atccagccgg
    16981 tgacgccgac caggacgacc ccggcgagga gcgccagcat gaccatgccc gcggcgggat
    17041 tgtgtccgag ccgccggcgc gacattccgg tcaggcgctc gtgcaggtac gcacgcaccg
    17101 cacgcggacc gcgcacccag tcgcggaagc gcgcatgcgg agtgccgacg aatccccaca
    17161 ggacgcgcaa cgcgaccagg cccatcgcga tgtatcccag cacgcgatgg gccgtgtcgc
    17221 cttcctcgac caggaacgca ccgaagacgc acgccgccag cgaccagtgg agaagccgca
    17281 ccagcggatc ccaaaccctg accgttggcg ccggctccgg gcggccggca tccctgccgg
    17341 ccgccgtctg gatgatcgtg tccatcgagg cgtacctttc acttgatgcg ctgcttgaca
    17401 atgtggccat cggtggggtc gaagtagatc tcgaccagct tgccggcctt gtccttgccg
    17461 tagatttcgt agcaggtgcc cgataccagg aacttgtcga tcgtgtaacc ctgcttgaca
    17521 aggcggtcct tcagcgcctg ctgcggcatc cactggcttt gcgcagcctt ggtgcaaacg
    17581 ggggcggcat atgcgcccag cgaaaacaac gcggcggagc cgcacagtac gagggttgcg
    17641 atttttttca tgagaggatt ctccgaaata cacgggcggg attgcccacg gcacgcaacc
    17701 cgatcagcga cctcgtgcgg cgctcgcgga ggggagcgag cgtcagtagt tgccgggcag
    17761 tgttcgaatc cgtgccacca ggatccatgg tgggctttct tgggccgcgc acgttgacgc
    17821 atgtcaagag gccacgcaca aaagtgcgtt cgcttgatgc acgtcaattg ccctgcagca
    17881 ggcaatccga tgatggagcc tcctacgagg agatcccacg atgaaaagcg aaaccgcccc
    17941 tgcgacgggc aacgaagagt ccttgaagcg tagcgtcgaa caagtcaagg atgccgcgga
    18001 aacgctgctg cggaccacgg tcggccaaac gggagaagag tggcgcaggg cgcgccatgc
    18061 atttggcggg aaggcgcgtg cgctcaggtc gagcatgtcc gggagtgcga aggagttcgc
    18121 cgaggaagcg cgggaactgg gaggcaaggg gcagcgcctt gtacagcagc acccctgggc
    18181 ctcgataggc atcggcgctg gcctcggctt gctggcgggg ctgcttgtcc gccgacgctg
    18241 agcggccatc cgcgaagggc gctgttcaag cgaagcggca atgtcagctc gcgaggcacg
    18301 cgcgtctgcc gaggcgccaa cgacggaagc gctggacggc cagtggcgcc tcgggctcag
    18361 cctggcggaa gcgcgacgtc ggctaatcga ttccggaccc aatgcgctgc cgggcggcga
    18421 acgcaagccg ctgcaccgga tcgtcctcaa ggtgctggcc gagccgatgt tcctgatgct
    18481 gctggtagcc ggcgcactgt atctggcgct gggcgaccac gccgaagcgg cgttcctgct
    18541 gggttcgatt ttcgccatca tcggcatcac gcttttccag gaacgcagga cccagcgcgc
    18601 actcgaagcc ctgcgcgacc tgtcggcgcc gcgcgcactg gtgatccgcg atgggcagcc
    18661 gttgcgcgtg ccaggtcggg aggtcgttcc cggcgacctg ctcgcgttgc acgagggcga
    18721 ccggatcccg gccgacgcca tcctgctgga aggcgagctc gaggtcgacg agtcgttgct
    18781 gaccggcgag gcgattgccg tggtccgcgc cgccggcacc gatcgcaacc gcgtgtacgc
    18841 cagcacggtg gtgacgcgcg gcgtggcgct cgcacgcgtg tcgtccaccg gaacagccac
    18901 gaaggtaggc gaaatcggag ccatgctgca gtcggcccgt gaaccggtgt ctggcctgca
    18961 gcgttcctcg cgcaagctca tccgtggcct ggccgccatc gcgctgctgc tggccatcgg
    19021 ctattgcctg accaactggc tgtggaatgg gcgaacgttc ctcgacagtt tgctcgccag
    19081 cgtcgcgctg agcatggcga tcctgccgga ggagataccc gtgatcctga cggtgttcct
    19141 cgcgatgggc gcctggcggc tgtcgcgcca ccgcgtgctg acgcgaagtg tccccgctgt
    19201 cgaggcgctc ggcgcgatca ccgtgctggc cgtggacaag accgggacgc tgacccagaa
    19261 ccgcatgcgc atcacagcac tggtgaccgc cgccggcgcg ctggaagaca ccccgtcgga
    19321 cgcgacgggt cccttgcctg cggccacgga agacctcgcc gaattcgcgt tgctggcaac
    19381 gccgtccgat ccgttcgatc cgatggaaac cgccatcctt gcgctcggcg accggctcga
    19441 cgactcgcgt cgcatgcatc gtggctggac gttgcagcgt gtctacgcac tttcctcgca
    19501 gacgcttgcg accacccgtg cctacgccgt ggcgggagaa gccggccatt gcgtcgcggc
    19561 gaagggtgcc ccggaaacga tcgtggcgtt gtgccggttg ccgcaggaat cggcatccgc
    19621 gatcctggat cgggctgccg cgttggcggc ccgtgggctg cgcgtactgg gcgtcgcccg
    19681 cggcgtctgg agcggaacga tgtggcccga gcggcaacag gatttcgcgt tcgaattcgt
    19741 cgggctgctg gggttcggcg atccgccgcg gccggaggtg ccggccgcga ttgccgagtg
    19801 caggagcgcc ggagtgcgcg tgctgatgct gaccggcgac catccggtca cggcacaggc
    19861 aatcgcgcgc gaaatcggat tgtcggaacg cgcggacgtg ctgaccggcg ccgaaatcgc
    19921 gcagcttggc gacgcctcgt tggccgagcg gctccggcag gtagacatct gcgcgcggct
    19981 gcgtcccgaa cagaaactgc gactggtgcg gctgctgcag cagtccggcg aagtggtggc
    20041 aatgaccggt gacggcgtca acgatgcacc ggcactcaag gccgcggacg tcggcgtcgc
    20101 aatgggggag cgcgggaccg acgtcgcgcg cgaggccgcc gcgctggtgc tgctggacga
    20161 cagtttcgcc agtatcgtcc gcgccatccg ccagggccgt cgcatctatg acaacgtcgg
    20221 caaggccacc gcgttcgtgt tcgcggtgca cgtcccgatc atcgtgctcg cgctgctgcc
    20281 ggcgctgctg cactggccgg tgatcctcgc acccgtccac atcgtgttgc tggaactgct
    20341 gatcgacccg gcctcctcga tggtgttcga ggtcgagccg ggaaggccgg acttgatgcg
    20401 acgaccacca cggccacgcg cgcacacgcc attcgcaccc ggccgactgg cgtacgcaat
    20461 atcgcagggt gccgggctgg ccgcgctgct gatcgcgggc tgcgccgtgc tgctgatgca
    20521 tggaggcgat gccgcgcaga cccgggcggc cgggttcgca gggttgctgg tgggcctgtt
    20581 cctgctggcg atggccgaaa gcagcttcgg gctcctgcgc cacgagcgcc gcaatccgcg
    20641 gctcaaatgg atgctgctgg gtgtcgcgac gaccctcgca ggcgtgttcg ggattccttc
    20701 gctacgcgcg ctgatgggtc tggcgacacc ggctttgcaa acgttggtgg ctgtcggggc
    20761 ggtcgtgctc gcgacgagtg gatggcttgc tgcgttgcag ctgggtgtac gcacgtttgg
    20821 gcaacgcggc tccggcaacg cgatcgtcag cgaatgacca gtaccggcac cctcgaccgt
    20881 gacagtacct tcatcaccac cgagcccagc actgcactca ggaaggcacc tcgtccgtgc
    20941 gagcccatgg caatgaggtc gcatcgctcc ttggtggcaa gtgacacggt gtacatggcc
    21001 gggtccccga cggcatgccg ctcctcgaac tcgaggccag ccttgcgcag cgcacggcgc
    21061 gccgggcgtg cagcgagttt ggagtgatcg tcgtggtaac ccgcgcgggt ctcggcggga
    21121 agaacccgtg ccacccgttc gggcagtggc gggtccacgt gcagcaggat caacgacacc
    21181 cgccgtttgg ccggcgtgcc gccccagtgc cggatgacgt agcgcacggc acgggaactg
    21241 cttgggcttc catcaacggg cagcaggatg ttcatggaaa tctcctcatt ggatggcggt
    21301 tttcatgccg gaccggacac ttgcaagaat gcgcgatgga ttcgactagg cgtttctgcg
    21361 gaccatccat gggcatcgcc gctggttgcg cagcgccaaa cgttcttgct gtcacccacc
    21421 tgcgaccgcg caacgcttga gcagcccgtg gggcaacgcc tcaccaatct cgctcgcaga
    21481 gaggcgcacc atgttctttg ccacccgcag cagaaccgtc tcgccgacgt gcgcaccgag
    21541 cgccgcattg agtgccccga ggaaccgggg cggcgcaatc agcaccatct cgcggtagcg
    21601 ccgccggccg tgggcatgct tcaggtacga ccctagcgag gcggcgaatt gcagggcagc
    21661 cttctccctc ggcgacgtgt gaacctcgat tgcatgccgt gcctccccaa cgcggtcgtg
    21721 tacgcgcggc ggtggcgcgt gctccggctc atggccggga gtacgcgcct ccggatcgac
    21781 gaagtcctcg aattcgatca tgcgcccagc gcgcctgttg atggaaaaca agcgcgcccg
    21841 cgcacggtcg gcaaccagga tccaggtggc cgccgcggtg tcctcaacgg aaggagaggt
    21901 aatcatcaac gcgctggacc ccgggtgccg accagactgc gtgctccacc gccttcattt
    21961 cgctccagtt gtccacccgg ccctcgatgc gcaccgtgcc gtcaccgcgc acatccacat
    22021 gaaccgcgtc ggcctccacc tcggcgtgcc gcttcagggc gtcctggatg cggcgtttca
    22081 gatcgccggc ttgcacggcc gaactcaggg tgatgctgtt ggtgacgccg ttgacgccgc
    22141 tcaatttgcg gacctcgttc tcggccgcct gtcgctggta gttccagttc atggttccag
    22201 acaacgtgat ccagccgccg gacaccctta cgcggacaca gtccttggga atcaaggtat
    22261 tccacgccag gatgttcagg gcgcgttggg cgatctcgtc gtcgctccat ttcttgtcgc
    22321 tgggcaaccg gacttcgatc ttctgtgcga tgcccttgac gcccttcacc cgccaggccg
    22381 cgcgctcggc cgccattttc tgcacgtagt cgggaacgtg gccactcagg gtgaccaccc
    22441 cgttttcggc cgtgacaccg atgtctgccg cgtcgatgct cggttcgtat tccaactcgt
    22501 cgatgacaag ctggcgtagt accttgtcgt tcatcactgt gctcctttcg gggttggagg
    22561 tttcatttca cacgttgcgg ccagtggttt cttgatccgc gtcaacaatg gatgacttgg
    22621 tcgaccgtgg tgggatcgtc gtacggagcg cgggtcgtga tgcgggaggc gcgcctttct
    22681 cggcacggcg catgcgtggc agcgtcacga acaacaccca catcagcaag acgcccgtga
    22741 caaacgcgca gacgagtatt ggtgacagga tcaggatggc ttgcatgtgc ggtatctcct
    22801 gcgaaacgac gttcgacgtt gcgacccaag ggtaggcaac acagtccttg ccggtttgat
    22861 ctcgatcacc ggaatgcgcg gtcaagcgac ggatgccgca ccttgaacca tcgccgggtt
    22921 cccggtgtcg gcacccatga agcggcgctt caccagctcc accagcagca gataggttgc
    22981 tgtggcgact gcgagaaagg cgaagaacac cgggggcagg ggaatgagcc ccagatcggc
    23041 tccgatggga gtgaacggca atgccgttcc agtgatcacc gcggcgaaca cggtggcgat
    23101 caatggcttg ctggggtggc tgcgcagcgg actgcccgcg gtgcgtatca caaagagcac
    23161 caaagtctgc gtggcaagcg attccacgaa ccagccggtg tggaattcgg cctgtgaggc
    23221 atgcagccac gcaaacaata catagaaggt aagaaagtca tagagtgaac tgatgggccc
    23281 gacccccagc atgaattgcc ggatcgatcg gacgttccag cggcgcggcg ctaccaattg
    23341 cgcgggatcc acgttgtcgc ccggaatggt gacctgggcc atgtcgtaca ggaaattgtt
    23401 gagcaggatc tgggtaggca gcatcggcag gaatggcaga acgagcgacg ctgcagccat
    23461 gctgaggacg ttgccgaagt tcgagctggt gcccatcagc aggtacttca tgacattggc
    23521 gaaggctttg cggccttcga tgacgccggc gtgcagggcc cccaggtcgc gctcgcccag
    23581 cacgatgtcg gcggcgtcgc gtgccacgtc cacggcattt gcgacggaaa tgcccacgtc
    23641 cgccgcatgc aacgatggcg catcgttgat accgtcgcca agaaacccca ccacggcgtg
    23701 ccgcgccttc agcgccagca cgatgcggtg cttctgcgcg gggctgagcc gcgcgaatac
    23761 gctgacgcgc tcggccacga caccgagcgc atccggcgca agcttttcga cttcgctccc
    23821 ggtcatcacc tcgcgagtgt ccagccccac ctgttcgcac acgtggcgga cgacgagctc
    23881 gttgtcgccg gaaaggatct tcacggcaac gccgtcgcgt gcgagcgcgc gcacggccgc
    23941 cgcgacatct ggcaatatcg gatcggcgaa cgacacaaaa ccggcgagga ccatcccggc
    24001 ttcatcttcc gcggcatagc gcggtcgccg gtcgatctcg cgatatgcga tcgcaagcgc
    24061 gcgcagcccc tgccgagcat atgcgtcgca gacgcggcgg gctgccgctt gtgcgtccag
    24121 ttcgagcgga cggaccacgc cgtcgactgc atatcccgtg caaagaggca gcacggactc
    24181 cggcgcgccc ttggtgacca gcagggcggt gtcgcccggg ccggtcgcca ccacactcag
    24241 gcgccgtcgc tcgaagtcga agggaatttc gtccagtttc cgggattcgt cctgcggcgg
    24301 ggtgcatccg cgcaggatcg cctcgtccaa cgggctgcga ataccggtct ggaatgaact
    24361 gttcagatgg gccagccctc cggcacgggt cgagggatcg ccgtacacat ccaccgcggc
    24421 gaacacatcc gtctcgttgc gggtcagcgt gccggtcttg tcgctgagca acacggtcat
    24481 gctgccgaag tcctcgatgg ccgcgaggtg ctttactacc acgccgtgcc gcgccatccg
    24541 ctttgcgccg cgcgccaggg tgacggtggt gatcatcggc atgaattcgg gcgtcagccc
    24601 caccgcgagc gccagcgcga acagcagggt ttccagcagc ggtcgatgga acacgatgcc
    24661 ggccagcacc accaacagca ccaggccgat caccgtgcgc atgatcagcc tggcaaaccc
    24721 ggccagtccg cgctcgaact cggtcggcgg cgggcgctcg gaaagacgcg cggcaacatc
    24781 gccgaaagcg gtatcgcggc cggtcgcgat cacgatggcg gtcgcgctgc cggcgaccac
    24841 cgaagtgccg aggtacacca ggtgtcgtgc atcggcgcca gccgtggcgc cgggctgctc
    24901 gcacacttcc ttttcggccg gtgcagattc gccggtcaag gccgattgct gcacgtgcag
    24961 gtcacgcgct tgcagcagcc gcgcatccgc gggcacgcga tcgcccgctg cgaggcgaat
    25021 cacgtcgcca ggcaccagcg tgcgccgggg cacgtcgcgc cacgcgccat cccggcacac
    25081 gctcgcggtc ggtgcgatct cctcgcgcag ccgctccgcc gcgcgcttcg agcgatagct
    25141 cagcacgtaa ttgagcgtca cgctcagcag caccatcgcc acgatgatgc ccgcaccgat
    25201 ccggtcgccg agaaagcccg acacgatcgc cgcaaccagc agcatcagca ccagggggtt
    25261 gccgaacagt tccatgagcg tttgcagggg accggcgcgg tcgcctgcgt gggcctcgtt
    25321 cgggccatgc tcggccagcc ggtgcgcggc ttcggcttcg ccaagcccgg ccgcgctgct
    25381 cgcgagccgc atgtacaggc tttcctgcgt gactcgtgcc ggatcgctcg cggcagctgg
    25441 gcggggggca tcgttcatgg cggccaacca tggcgcggcg gtacgaatgg atattgacgc
    25501 gggtcaagcg ccgaggcatc tctccgaatg aagatggagt tgacgtgcat caacatggct
    25561 gggcggggag cgcgtagcgt ccattgcgtg cgcaggtgca ggaccgcaat cgaggagttc
    25621 caccatggca gcaatcacgc gcttcaaccc attccggagc ccggcacgat tcgaaacttc
    25681 cggtgcgttc gacgacttgt tccgcaacct gggattcggc tcgtcgtgga gccagcccga
    25741 attcatgtcc gacatgcggg tggatatcac cgaggacgac gatgcctact acatcgacgc
    25801 cgaaattccc ggcgtaaaca aggacgatat cgacgtatcc gtggaaggaa atcgcgtggc
    25861 gatcaacgcc gaagcgaaac gcgaaaagca gaagaaggac gaaaaggagc tcatggtcga
    25921 gcgttcgtgg ggcagggcct accgtgcctt cgcactcccg ggcgacatcg acggcaatcg
    25981 cacggaagcg cgttacgaca agggcgtgct tacgctcatg cttcccaaga atgcgaatgg
    26041 aaacgcccgc cggatcgcgg tgagctgatt cacgccaagt ggtttgtcgg ggccgccgtc
    26101 acgaggggac ggcggctttt ttgctgccgg acaacggcga agcgaagggc cacactgcca
    26161 cgatccgata acggggcagg ggatcgcaag cggcgagttc agtcgactgt tgcccgtacg
    26221 tggctcttga cgtcgccgcg acgacgacgc acctgatcct cgatccggcg gcgcaacacg
    26281 tcgaaggtgt gtgctgcggc ctcgtaagga tcttcgtggt cggaatccgc tccgggcgta
    26341 cggcccgcct cgatttcgcg atcgcgcatt gccaccctcg cgtggacgtt gtagcgattg
    26401 ccatgccggt gcctgcgctc acacgcccgc accaccacgt cgcacgtctg gatgtcatcc
    26461 gcataccgct gcagccgctc ggcacgcgcc tcgattttgg cgcgcagcgc ttcggaagca
    26521 tccatgccct ggaatgtgac gcgaacggga atcttcatga cctgtgggac ctcggctgga
    26581 ccgtttgtcg attctgaggg ccggcgcagc gcgcctgttg atatacgtca ggtcgaaacg
    26641 gggcaactgt ctcgcgatcc cttgacgagg gtcaataccg ggtatcggca acccgtcacg
    26701 ctggcgtcgc gttcgagcac ctcgacgccg agttcccagg aaccatggag cgcaacatga
    26761 aagacatcct cgtttatgtc agggattttg aggagcgcac gcccgccgcg catttcggcg
    26821 tgaggctcgc cgccacgttc ggtgcatccg tcaccggcgt ttacacctgc gcccaaccgg
    26881 tctatctcgc accagcgtac gaacccgagc tgatggcgag ggccgtcgaa gacgcgcgcg
    26941 agctcgccaa ggatgcggta cagagcaagc gatcattcct cgactgggcc acctctctgg
    27001 gtgtaccgca aaccgagtgg ctggtcgtcg aaggcgatgc ccgcgacgcc ctggccgagg
    27061 cggcgacacg ccacgacctg ctggtgctcg accacgccga ggaaggccgc gggtcgtcgt
    27121 gggacattcc gggcctggtg ctgaagacgg acattccatg cgtcgtcctg ccacaccatg
    27181 gggtgcatta cacccagttc gaacggatcg cgatcggctg gaacggctcg ccggaagcca
    27241 tgcgcgcggt gcacgcggcc ttgccattcc tgaaaggcag gcaggcgttg ttgatgcgcg
    27301 gcgaagaacg cgacaagtat ccggggctgg aatgggaccc cccgttcaac atcatggact
    27361 acctcaagcg ccacggtgtc acggcagaac aacgcacgat ccgcgcgaag cacgatgatg
    27421 tgggtgaggt gctgctggac gaagccatga agttccgcgc cgacatgctg gtgatgggcg
    27481 cctacggacg cagccggttc agcgaatgga tgctgggcgg cgcgacccgc catgtgctga
    27541 cgtgggccga cattccggtc ctcatgcggc actgacggtc cgtgcgggtc acagccggat
    27601 gcgcgcgatg gtgctggagt cagtcggtac gcggctcgtg ttgaaggagt tggcgaatcc
    27661 cgccttgcat cccggcgaag tgctcgtgcg ggtgctggcg tgtggcatct gtcgcaccga
    27721 cctccacgtc gtggacgggg aactgccacg cgtgaggccg ccggttgtcc ccgggcacga
    27781 agtcgtcggg atcgtggagc aggtggggga aggcgtggag gctgtcgcgc cgggagcgca
    27841 cgtcggcatt ccgtggctcg gcggcacctg cggtgaatgc gcatactgca gggccgggcg
    27901 cgagaatcta tgcga
//
LOCUS       DASSNH010000167         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1204032, whole genome shotgun sequence.
ACCESSION   DASSNH010000167 DASSNH010000000
VERSION     DASSNH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204032"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /gene="gcvPB"
                     /locus_tag="VFK00_06300"
     CDS             <1..1478
                     /gene="gcvPB"
                     /locus_tag="VFK00_06300"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET7592600.1"
                     /translation="RQLADKDLALDRSMIPLGSCTMKLNATAEMIPITWPEFAQIHPL
                     APPEQWRGYHELIDGLEAMLVALTGYDAVSLQPNAGAQGEYAGLLAIRAYHQANGQPQ
                     RDVCLIPESAHGTNPASAQMCGMTVVVTKCDGNGNVDVDDIRAKAGKYSDRLAAIMLT
                     YPSTHGVFEEEIVTICDIVHQHGGQVYTDGANLNALCGVARPGRWGSDVSHLNLHKTF
                     CIPHGGGGPGVGPCAVKSHLAEYLPRAFSSAGIPAGEHQEPAIHGTTSHNSRSGGMVS
                     AATFGSASILPISWMYITMMGREGMLKATQVALLNANYLSKRLAEHYQTLYTGRNGLV
                     AHECILDLRPLEKSSGVTAEDVAKRLVDFGFHAPTLSFPVPGTLMVEPTESESLHELD
                     RFIDAMIQIRDEIRAIEDGRLDREDNPLKNAPHTATMVSGSEWSHAYPRELGAYPLAS
                     LKRTKYWPPVARVDNVYGDKHVFCSCVPIAAFAEEDATTAP"
     gene            complement(1557..2453)
                     /locus_tag="VFK00_06305"
     CDS             complement(1557..2453)
                     /locus_tag="VFK00_06305"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HET7592601.1"
                     /translation="MDRGVAASTSTVPDIERLAEWLAQRSRVAVITGAGVSTESGIPD
                     YRDGDGNWKRSPPVQYRDFVGSAAVRKRYWARSFAGWPMFAAARPSLAHVALARLEQA
                     GRIAEVVTQNVDRLHQRAGSRKVIDLHGRLDVVRCLANGHRFDREAFQARLREENPDW
                     DAATTRIAPDGDADLEGVDFDAFVVPPCDVCGGLLKPDVVFYGESVPREVGGAALAAI
                     ESADGLLVAGSSLMVWSSFRLVRAAVQRGIAVVAANRGRTRADDLFAFKLDGECGEVL
                     NATLDALHPPAPAARPPSFRRG"
     gene            2763..>4474
                     /locus_tag="VFK00_06310"
     CDS             2763..>4474
                     /locus_tag="VFK00_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7592602.1"
                     /translation="MPANKLQLQASTAWPPVASAALRVAFGIIWAVGAVLTFSPDFAV
                     HYVGYLHNAAQGQPAWSAWWFNGWITIVTPHVMLFVWLTRIVESALAIALLVGFARKT
                     TYVLGALFSLLVWSTAEGFGGPYTVGATNMGTAITYVLIFIALIGINARGGRSPYSAD
                     YFIEKRWPGWRRVAEWGGPDTLAQEPVVLPWRMQVPAVLGVVLLLVLLILGLESAFHV
                     RSPSPVAAAAAVTPLSLAKAEPTAPPRDARLPPLIGTGDSVDVHIEATDDSVAIANGV
                     DYQAWTFGGSVPGPILHVRQGQTVNVTFTNHGNMKHSLDFHSAVTPPSVHYVDIDPGQ
                     TITYSFVANTPGAFLYHCGTPPVLLHIGNGMYGAIIVDPATPLPPAAASYVLVQGEWY
                     TQQVSGTTMGPSFEKMQEERPDEVVFNGAAFQYRDHPLPVTAGQRVRLYVVNAGPNLW
                     SSFHVIGAIFSEVYPDGDATHAQTGVSTYTIGPGAGAIFDLVLPEPGEYPFVDHDMAH
                     AGKGAIGVLDARGANGEAPAKPQITSAPASAAPAVATPAAAPAGPYKFDAAKGAELYG
                     ARCAA"
BASE COUNT          751 a         1640 c         1410 g          673 t
ORIGIN      
        1 tgcgccagct cgccgacaag gacctcgcgc tcgaccgcag catgatcccg ctcggctcgt
       61 gcaccatgaa gttgaacgcg accgccgaga tgatcccgat cacctggccc gaattcgcgc
      121 agatccatcc gctggcgccg cccgaacagt ggcgcgggta ccacgaattg atcgacgggc
      181 tggaagccat gctggtcgcg ctgaccggct acgacgcggt gagcctgcag ccgaacgccg
      241 gcgcgcaggg tgagtacgcg ggcctgctcg cgatccgcgc ctaccaccag gcgaacggcc
      301 agccgcagcg cgacgtgtgc ctgatcccgg aatccgcgca cggcaccaat ccggcatcgg
      361 cgcagatgtg cggcatgacc gtggtcgtca ccaagtgcga cggcaacggc aacgtggacg
      421 tcgacgacat ccgcgcaaag gccgggaaat attccgatcg ccttgccgcg atcatgctga
      481 cctatccgtc cacccacggc gtgttcgagg aagagatcgt cacgatctgc gacatcgtcc
      541 accagcacgg cggtcaggtg tacaccgacg gcgcgaacct caatgcgttg tgcggtgtcg
      601 cgcgccccgg ccgctggggt tcggacgtgt cgcacctcaa cctgcacaag accttctgca
      661 tcccgcacgg cggtggcggc cccggggtcg gtccatgcgc agtcaaatca cacctcgccg
      721 aataccttcc acgcgctttt tcatcagccg gcatccctgc cggcgaacat caagagccgg
      781 ccatccatgg cacgacttct cacaacagcc gctccggagg aatggtttca gcagcaactt
      841 tcggctcggc gtcgatcctg ccgatctcgt ggatgtacat cacgatgatg ggccgcgaag
      901 gcatgctgaa ggccacccag gtcgcgctgc tcaacgccaa ctacctgtcg aagcgactgg
      961 ccgagcacta ccagacgctc tacaccggtc gcaacggcct ggtcgcgcac gagtgcatcc
     1021 tcgaccttcg tccgctcgaa aagagcagcg gcgtcaccgc cgaggacgtc gccaagcggc
     1081 tggtcgactt cggcttccac gcgcccaccc tcagcttccc ggtccccggc acgctgatgg
     1141 tcgagccgac cgaatcggaa tcgctgcacg aactcgaccg cttcatcgat gcgatgatcc
     1201 agatccgcga cgaaatccgc gcgatcgagg atggccgcct cgaccgcgag gacaacccgc
     1261 tcaagaatgc gccccacacc gcgaccatgg tgtccggaag cgaatggagc cacgcctacc
     1321 cgcgcgaact cggcgcgtac ccgctggcgt cgctgaagcg cacgaagtac tggccgccgg
     1381 tcgcgcgcgt ggacaacgtc tacggcgaca agcacgtgtt ctgctcgtgc gtgccgatcg
     1441 ccgcgttcgc ggaagaagac gccacgactg cgccctgaac ccggagcaca tccccctcgt
     1501 cgcgctgcgc gcgctcgctt cgctccgccc ttcgcaagcg aagggggaag aaaagctcac
     1561 ccccttcgga acgaaggggg tcgcgccgca ggcgcggggg gatgaagtgc gtccagtgtg
     1621 gcgttcagca cttccccgca ctcgccgtcc agcttgaacg cgaacaggtc gtcggcgcgg
     1681 gtgcggccgc ggttcgcggc gacgaccgcg ataccgcgtt gcaccgccgc gcggaccagc
     1741 cggaaactcg accacaccat cagggaagag ccggcgacca gcaggccgtc ggccgattcg
     1801 atcgcggcaa gcgcggcccc gccaacctcg cgcggcaccg attcgccgta gaacacgaca
     1861 tcgggtttca gcaggccgcc gcagacgtcg cacggcggaa ccacgaacgc gtcgaagtcg
     1921 acgccttcca gatccgcatc gccatccggt gcgatccggg tggtcgcggc gtcccaatcg
     1981 ggattctcct cgcgcaggcg cgcctggaat gcttcccgat cgaatcgatg cccattcgcg
     2041 aggcagcgca ccacgtccag ccggccgtgc aggtcgatca ccttgcggct gccggcgcgc
     2101 tggtgcaggc gatcgacgtt ctgggtcacc acttccgcga tacggcctgc ctgttccagc
     2161 cgcgccaatg cgacgtgcgc caaactcggc cgcgccgcgg cgaacatcgg ccagccggcg
     2221 aagctgcgcg cccaatagcg cttgcgcacc gcggcgctcc cgacgaagtc ccggtactgc
     2281 accggcggcg agcgtttcca gttgccgtcg ccgtcgcggt aatcggggat gccggattcg
     2341 gtgctgacgc ccgcgccggt gatcacggcc acgcgcgagc gttgggccag ccattcggcc
     2401 aggcgctcaa tgtcgggaac cgtcgaggtc gaggcggcga cgccgcggtc catcgtcaac
     2461 ctccatccaa gagcttgcgt ccactctagc gcgaatgcac ggatcggctc ggcgtccgcc
     2521 agagcccacc acggcccgac gatgcccgcg ctcacccctt gatgcaacgt taacgcccgt
     2581 gcctccacga tgcacgttcc gctgcagacc acacgcatgc caggcgcaac ccggttgccg
     2641 ccggctttga ccgcggtcaa accgccggcg cactgcgtct gccatgctgt tgcggaggcc
     2701 gggtggttcg cccccgggcc gcaggactct ccaataattc caatcggaaa aggaacctgt
     2761 ccatgccagc gaacaagctc cagctccagg cctccacagc gtggccgcca gtcgcctccg
     2821 ccgcgctgcg ggtcgccttc ggcatcatct gggcggtcgg cgccgtgctc acgttctcgc
     2881 cggatttcgc cgtccactac gtcggctacc tgcacaacgc ggcgcaaggc cagccggcct
     2941 ggtcggcgtg gtggttcaac gggtggatca ccatcgtcac gccccacgtg atgctgttcg
     3001 tgtggctcac ccgcatcgtg gaaagtgcgc tcgcgatcgc actgctcgtg ggcttcgccc
     3061 gcaagaccac gtacgtgctc ggcgccctct tcagcctgct ggtgtggagc acggccgaag
     3121 gcttcggtgg cccctacacg gtgggcgcga ccaacatggg caccgcgatc acctacgtgc
     3181 tgatcttcat cgcgctgatc ggcatcaacg cccgcggcgg ccgcagcccg tacagcgccg
     3241 actacttcat cgagaagcgc tggcccggct ggcgccgcgt ggcggagtgg ggcggacccg
     3301 acaccctggc gcaggaaccg gtggtgctgc cctggcgcat gcaggtcccg gcggtgctcg
     3361 gcgtcgtgtt gctgctggtc ctcctgatcc tggggctgga gagcgcgttc cacgtgcgct
     3421 cgccctcgcc cgtcgcggcc gccgccgcgg tcacgccgtt gtcgctggcc aaggccgagc
     3481 cgaccgcgcc gccgcgcgat gcgcgcctgc cgccgctgat cggcaccggc gacagcgtcg
     3541 acgtccacat cgaagctacc gacgattcgg tcgcgatcgc caacggcgtc gactaccagg
     3601 cctggacctt cggcggcagc gtgccaggcc cgatcctgca cgtgcgccag ggacagacgg
     3661 tcaacgtcac cttcaccaac cacggcaaca tgaagcactc gctcgacttc cattccgcgg
     3721 tgacgccacc gagcgttcac tacgtcgaca tcgacccggg ccagaccatc acgtattcgt
     3781 tcgtcgcgaa cacgcccggc gcgttcctct accactgcgg cacgccgccg gtccttctgc
     3841 acatcggcaa cggcatgtac ggcgcgatca tcgtcgaccc cgccacgccg ctgccgccgg
     3901 ccgccgcgag ctacgtgctc gtgcagggcg agtggtacac gcaacaggtg tccggcacca
     3961 ccatgggccc gagtttcgaa aagatgcagg aggaacgccc cgacgaagtg gtcttcaacg
     4021 gcgccgcgtt ccagtaccgc gaccatccgc tcccggtgac cgccggccag cgggtacggc
     4081 tgtacgtcgt caacgcaggg ccgaacctgt ggtcgtcgtt ccacgtgatc ggagcgattt
     4141 tcagcgaggt ctatccggac ggtgacgcga cccacgcgca gaccggcgta tccacctaca
     4201 cgatcgggcc gggcgccggc gcgatcttcg acctggtgct tccggagccc ggcgaatatc
     4261 ccttcgtcga ccacgacatg gcgcacgcgg gcaagggcgc gatcggagtc ctcgacgctc
     4321 gcggcgccaa tggcgaggcg cccgccaagc cgcagatcac ctccgcgccc gcctccgcgg
     4381 cacccgcggt ggcgacgccc gcggcagcgc cggccgggcc gtacaagttc gatgccgcca
     4441 agggcgccga gctgtacggc gcgcgctgcg ccgc
//
LOCUS       DASSNH010000168         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1257304, whole genome shotgun sequence.
ACCESSION   DASSNH010000168 DASSNH010000000
VERSION     DASSNH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257304"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..2321)
                     /locus_tag="VFK00_06315"
     CDS             complement(15..2321)
                     /locus_tag="VFK00_06315"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HET7592603.1"
                     /translation="MLQRVFVVLIALVFSFSAQAKGAAPAAVCPASSSSVVAAPPAQY
                     KATPAPVLKPTAAQGQAAILAARLLTRFQYEALPLDDAMSQKIFDAYFKALDGSRLFF
                     TQQDLDQFAADRTKLDDAIWNGDMSIPFAIFNLYEKRAGERTAYALALLKKGYNPDTN
                     DIYTFDRDKAPWAADTAALDKLWQGRVQNDWIRLKLAGKDDAEIRKTLSRRYENYLDR
                     VHQLDSEDVFQTFMNAYATAIDPHTNYFGPRASQNFDITMKLSLEGIGAVLQEHDEYT
                     VIREIVPGGPAAKSGKLEVGDKIVGVGQGECGAIVDTIGWRIDDVVAKIRGTKGTVVR
                     LEIVPAEGGPDAKPRLITLVRAKVTIADQAAKKKVIDVKEGDRTIKVGVIDLPSFYED
                     FDARRRGDPDYRSASRDVAKLLGELKQEKVSAVIVDLRDNGGGSLSEAVNLTGLFTGK
                     GPVVQVRDSRGQVDEQDSDEKMLWTGPMAVLVNRGSASASEIFTAAIQDYGRGLIIGE
                     QTFGKGTVQTLTSLDQLAMNTKPTFGELKMTVDEFFRVNGDSTQLHGVTPNILFPHSI
                     GYKDYGESSYPNALKWTHIDPAAYTPVATQQPLVAPLTAEHDARAKTDPAWQLLLDEL
                     AAARKLHDQKTVSLNYKVREAERKQQEEQDAAFKKRREALGDVSGVNLQLDNGLTPGE
                     QSVKASVAREKAAREAKDIELDEAAHVMADEVVMLEKDPKLAQQVLPKQAFTKPVSIA
                     TSAPVSASAPAAESSSSEHLPASAGSTH"
     gene            complement(2502..>2995)
                     /gene="lipB"
                     /locus_tag="VFK00_06320"
     CDS             complement(2502..>2995)
                     /gene="lipB"
                     /locus_tag="VFK00_06320"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7592604.1"
                     /translation="HVLAPGDIPVLHVDRGGQVTYHGPGQIVAYPLLDLRRLKLGVRE
                     LVLRIEQSIIDTLAEWNIVAARREGAPGVYVADAKIAALGLRVRHGCTFHGLAFNIAM
                     DLEPFHRINPCGYAGLQVTQMLDLGGPSRLADVEEVLIAELGRQFGLIPQDAESVLPR
                     AAA"
BASE COUNT          452 a          958 c         1002 g          583 t
ORIGIN      
        1 ggcagcgcat gcgttcagtg cgtgctgccg gcgctggcgg gcaggtgttc cgatgaggag
       61 gattccgcgg ctggtgccga tgccgaaacg ggcgccgagg tggcgatgga aaccggcttg
      121 gtgaaagcct gtttcggcaa cacctgctgg gcgagcttcg gatccttttc cagcatcacg
      181 acctcgtcgg ccatgacgtg cgcggcctcg tccagttcga tgtccttggc ttccctggcg
      241 gccttttcgc gcgccaccga agccttgacg ctctgctcgc ccggcgtcag tccgttgtcg
      301 agttgcaggt tcacgcccga cacgtcgccc agtgcctcgc gacgcttctt gaacgcggcg
      361 tcctgttctt cctgctgctt gcgttcggcc tcgcgcacct tgtagttgag cgacaccgtc
      421 ttctggtcgt gcagcttgcg cgcggcggcc agctcatcca gcagcagttg ccacgcggga
      481 tcggtcttcg cgcgcgcgtc gtgttcggcg gtcagcggcg caaccagcgg ctgctgcgtc
      541 gcgaccggcg tgtacgcagc cggatcgatg tgcgtccact tcaatgcgtt cggatagctc
      601 gactcgccgt aatccttgta gccgatcgaa tgcgggaaca ggatgttggg cgtgacgcca
      661 tgcaactgcg tggaatcacc attgacgcgg aagaactcgt ccaccgtcat cttcagctcg
      721 ccgaaggtcg gcttggtgtt catcgccaac tggtcgaggc tggtgagcgt ctgcacggtg
      781 cccttgccga aggtctgctc gccgatgatc aggccgcgtc cgtaatcctg gatcgcggcg
      841 gtgaagatct cgctggcgga cgccgagccg cggttcacca gcaccgccat cggaccggtc
      901 cacagcattt tctcgtccga atcctgctcg tcgacctggc cgcggctgtc gcgcacctgc
      961 accaccggtc ccttgcccgt gaacaggccg gtgaggttca ccgcttcgct gagcgaaccg
     1021 ccgccgttgt cgcgcaggtc cacgatcacc gcggagactt tctcctgttt cagttcgccc
     1081 agcagcttgg cgacgtcgcg cgaggcgctg cggtagtcgg gatcgccgcg gcggcgcgcg
     1141 tcgaagtcct cgtagaaact cggcaggtcg atgacgccga ccttgatggt gcggtcgcct
     1201 tccttgacgt cgatgacctt cttcttcgcg gcctggtcgg cgatggtgac cttcgcgcgc
     1261 accagcgtga tgagcctggg cttggcgtcg ggaccgcctt ccgcgggcac gatttcgagc
     1321 cgcaccacgg ttcccttggt gccgcggatc ttggcgacca cgtcgtcgat gcgccagccg
     1381 atggtgtcga cgatcgcgcc gcattcgccc tggccgacgc cgacgatctt gtcgcccact
     1441 tcgagcttgc ccgacttcgc agccgggccg cccggcacga tctcgcggat cacggtgtat
     1501 tcgtcgtgct cctgcagcac cgcgccgatg ccttccagcg agagcttcat cgtgatgtcg
     1561 aagttctggc tggcgcgtgg accgaagtag ttggtgtgcg gatcgatcgc ggtcgcgtac
     1621 gcgttcatga aggtctggaa cacgtcctcg ctgtcgagct ggtgcacgcg atcgaggtag
     1681 ttctcgtagc ggcgcgacag cgtcttgcgg atttccgcat cgtccttgcc cgcgagcttc
     1741 aggcggatcc agtcgttctg cacgcgtccc tgccacagct tgtcgagtgc ggcggtgtcc
     1801 gcggcccacg gcgccttgtc gcggtcgaag gtgtagatgt cgttggtatc ggggttgtag
     1861 cctttcttca gcagcgccag tgcatacgcg gtgcgttcgc ccgcgcgctt ttcgtacagg
     1921 ttgaagatcg cgaacgggat cgacatgtcg ccgttccaga tcgcatcgtc gagcttggtg
     1981 cggtcggccg cgaactgatc gagatcctgt tgcgtgaaga acagcctgct gccgtcgagc
     2041 gccttgaaat acgcgtcgaa gatcttctgc gacatcgcgt cgtcgagcgg cagcgcctcg
     2101 tactggaagc gcgtcagcag gcgcgccgcc aggatcgcgg cctggccctg cgcagcggtc
     2161 ggcttcagca ccggtgcggg cgtcgccttg tactgggcgg gcggcgcggc gaccacgctg
     2221 gacgaagaag cggggcagac ggccgcgggc gcggcgcctt ttgcttgcgc gctgaacgaa
     2281 aagacgagag cgatcaggac aacgaagaca cgctgcaaca tggactttcc ttgggcggcc
     2341 gcaacggtgt ggccgtttgc gctcggagcg cggtcaaaga cgaaagatgc gccagccgtt
     2401 cgctggtgcg tgcgggtgcg tggtgcaggc ggaaaaggcc cgtccgggcg cggcccggca
     2461 ttaaagtatc ggaaaaaaca ggcgccggat atggccgcaa gtcacgcagc ggcacggggc
     2521 aacaccgatt ccgcgtcctg cgggatgagt ccgaattgtc ggccaagctc ggcaatcagc
     2581 acttcctcga catcggcaag ccgcgacggg ccgcccaagt ctagcatctg cgtcacctga
     2641 agacccgcgt agccgcaggg attgatgcgg tggaacggct ccaggtccat cgcgatgttg
     2701 aacgcgagtc cgtggaaggt gcaaccgtgg cgtacgcgca ggcccagcgc cgcgatcttg
     2761 gcgtctgcca cgtacacgcc gggcgcgcct tcgcggcgtg ccgccacgat gttccattcg
     2821 gccagggtgt cgatgatgga ttgttcgatc ctgagcacca gttcgcgcac gccgagcttc
     2881 agccgccgca ggtccagcag cggataggcc acgatctggc ccggaccgtg gtaggtcacc
     2941 tgcccgccgc ggtcgacatg gaggaccggg atgtcgcccg gtgccagcac gtgtt
//
LOCUS       DASSNH010000169         7339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1587593, whole genome shotgun sequence.
ACCESSION   DASSNH010000169 DASSNH010000000
VERSION     DASSNH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587593"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFK00_06325"
     CDS             <1..592
                     /locus_tag="VFK00_06325"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET7592605.1"
                     /translation="KIKNEIDPTLTLRRSCREGVCGSCAMNIDGENTLACTKGLDELP
                     AGDVSIYPLPHMPVVKDLVPDLTHFYAQYASIKPWLQSQSSFGARERLQSPEDRKKLD
                     GLYECILCACCSTACPSYWWNPDRFLGPAILLQAYRWIVDSRDEATGERLDDLEDPFK
                     LYRCHTIMNCTNTCPKGLNPAKAIAGIKQMLVNRTV"
     gene            650..1228
                     /locus_tag="VFK00_06330"
     CDS             650..1228
                     /locus_tag="VFK00_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592606.1"
                     /translation="MIAPERPPYEAEPTIHWLVRGVMWLLVLLFVGLPCFLVAAPFLH
                     PAGPAPSWTVWSALEVAGVVCGCCVAGAGFLGTLAWRGHSDIAGVAWIDNHPVSPRLR
                     WYTRVWAGVVCAGVMMLLVAQLHRLLVGDASWLSVSMTFVMVAVVLGYLAAPLVSGRW
                     SEAAMRSALRLTMTPWAIHAAVKAEHDGRRGI"
     gene            1206..1475
                     /locus_tag="VFK00_06335"
     CDS             1206..1475
                     /locus_tag="VFK00_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HET7592607.1"
                     /translation="MTAGAASDDRLKRLRWRCRRGTRELDALLGGWLDARRDSIDAGE
                     LATFDALLDQQDPVLWDWLMGHAEPPRADWRAIVAEIRERAGLPQ"
     gene            1472..1921
                     /locus_tag="VFK00_06340"
     CDS             1472..1921
                     /locus_tag="VFK00_06340"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HET7592608.1"
                     /translation="MKSAPAVGFDYHASRSLILATLVVAFFAVAAVWLSGLPWLAQLA
                     LTLGIVAAAGVAFGNLVRPRVRSMRWRSDGGADICLGDDRREAQGEVSAARVMGPLIA
                     LSLRWPPRGRATLWLLPDNLDPETRRRLRMRLGAVAAHPLSGNTDNA"
     gene            2051..3307
                     /locus_tag="VFK00_06345"
     CDS             2051..3307
                     /locus_tag="VFK00_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET7592609.1"
                     /translation="MFRPLELYIGTRYIRAKRRNHFISFISIVSMLGLVIGITALITV
                     ISVMNGFDGELRSRILGMVSQATISGVGNDMPNWQQALAIAEQNPHVRGAAPYVEQYG
                     LLRGNAANQGAVIRGILPDQEPKVADIGSKMIAGKLSSLTDGSWGIVLGRDLALALGV
                     RPGDKIVVYATEVTANPITGVVPRLKRFTVTGIFEAGMQEYDSGLAIVNLHDAEKLYR
                     MDGPTGIRLKLDDLFAAGRVAQNLQDQFGEMGAAYRAQGWMQSHANFFKAIAMEKIVM
                     FIILSLIVAVAAFNLVSSLVMLVTDKQSDIAILRTLGATPRSIMGVFMIQGMLIGIMG
                     IVVGVVCGVALAINLPSIVDWIQNITGYQFLSESVYYINNVPSQLHWGDVGWITGMAF
                     LFSLLATIYPAWRASRTQPAQALRYE"
     gene            3300..4028
                     /gene="lolD"
                     /locus_tag="VFK00_06350"
     CDS             3300..4028
                     /gene="lolD"
                     /locus_tag="VFK00_06350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HET7592610.1"
                     /translation="MNSREQGAGSRGLGNGVVLRASHIAKTYKEGSLRTPVLEDASFE
                     LARGETLAIVGASGSGKSTLLHIVGGLDTPTKGMIELEGRELSKLPDAERGRVRNRSL
                     GFVYQFHHLLPEFSALENVAMPLLIRGTPISDAREQATTLLERVGLGHRLEHKPGELS
                     GGERQRCAMARALVTRPSCVLADEPTGNLDEQNAASLYELMLELNREIGTSIVLVTHD
                     RKLAAQMDRVLELTGGALHPLPDD"
     gene            4036..6399
                     /locus_tag="VFK00_06355"
     CDS             4036..6399
                     /locus_tag="VFK00_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET7592611.1"
                     /translation="MDTRRRERRVVRTTPGILAGAMAFLAGAVLVQALPVLPPRWLDA
                     VWGCAALAGLGLCLRKRLPGGVLFVAILLGAFAWTAWRADLALGARLPHALEKQDILV
                     TGRVTDLPEPREDSTRFDFEISNAHFDGRPIAVRGHVRLSWYATRGHAPPTIAPCSTW
                     RLRVRMKRPHGLVNPGGMDFERSALQKGIVATGYVREDPANAMLDAGACVDGVRARIG
                     GAIDAALPNDAHAARLLRALSVGDERALDEHDWQVVRTTGISHLIAISGFHVGLAAVF
                     GALLVRLLWWLFPALALRLARPLAEATVAFPAALAYGALAGFGLPTTRTLLMIAVVAG
                     WRLLRRGGGFGEGFGLALAAILVLDPLSVLSAGFWLSFAGVALLAWTLARDSGWRGHL
                     KEIGFAPLLMTLALLPLTVWFFGQASLVGPIANLVAVPFVSFVIVPIDLAACALLFAW
                     PAAGQGLLHVCAHLVDGLWWFLVHLAAWKPAMQYLPQPTFLAFALACIGAVWLLAPRG
                     VPARALGALLLLPLCWPRASLPSAGAFRATVVDVGQGLSVLVRTRGHALLFDTGARYP
                     SGFDLGAAAVVPSLHGLGVTHLDRLIISHGDNDHAGGAASVLAAYPGTPVWGGEPARG
                     PVPMRQCHAGQHWQWDGVAFRMLRPPAPVAIDGNDAGCVLLVSGKDGRLLLPADTSSK
                     VEPDIARAVPPGPPLVLVVPHHGSKTSSSRAYLEALAPRFAIASTGYLNGYHHPAPVV
                     AARYAKLGIPLLNTPATGAVRIAFPANAPPRILSEARIRQAHYWREH"
     gene            6924..>7339
                     /locus_tag="VFK00_06360"
     CDS             6924..>7339
                     /locus_tag="VFK00_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006429918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7592612.1"
                     /translation="MFEILKAGGWGMLPILVCSAVGLAIVLERFWTLRRGTVLPPGLG
                     DQVRSWAHSQQLTSSHIQALRENSPLGELLAAALDVRNRSRSEIKERIEDTGRHVVHD
                     LERYLNTLGTIALIGPLLGLLGTVFGLIRMFLAVMVS"
BASE COUNT         1045 a         2405 c         2541 g         1348 t
ORIGIN      
        1 gaagatcaag aacgagatcg atccgacgct gaccctgcgc cgttcctgcc gcgaaggcgt
       61 gtgcggctcg tgcgcgatga acatcgacgg cgagaacacc ctcgcgtgca ccaagggcct
      121 cgacgaattg ccggcgggcg acgtcagcat ctatccgctg ccgcacatgc cggtggtcaa
      181 ggacctcgtc cccgacctca cccatttcta cgcgcagtac gcctcgatca agccgtggct
      241 gcagtcgcag agttcgttcg gtgcgcgcga gcgcctgcag tcgccggaag accgcaagaa
      301 gctggacggc ctgtacgagt gcatcctgtg cgcgtgctgc agcaccgcct gcccgagcta
      361 ctggtggaat ccggaccgct tcctcggccc cgcgatcctg ctgcaggcgt accgctggat
      421 cgtggactcg cgcgacgagg cgaccggcga gcgcctcgac gacctcgagg acccgttcaa
      481 gctgtaccgc tgccacacca tcatgaattg caccaacact tgtccgaaag gcctgaatcc
      541 tgccaaggcg attgcgggca tcaagcagat gctcgtcaac cgcacggtgt gaccggacgg
      601 ttgcacgttc agagcgtaca atctggtgca gccgcacaag gaggtaacca tgatcgcccc
      661 tgagcgcccg ccgtacgaag ccgagccgac catccactgg ctggtgcgtg gcgtgatgtg
      721 gctgctggtc ctgctgttcg tggggttgcc gtgcttcctg gtcgccgcgc cgttcctgca
      781 tccggcggga cctgcgccgt catggacggt gtggtccgca ctggaagtgg cgggtgtcgt
      841 ttgcggttgc tgtgtcgccg gtgcggggtt cctgggcaca ttggcctggc gggggcattc
      901 cgatatcgca ggcgttgcct ggatcgacaa ccatccggtg tcgccgcggc tgcgttggta
      961 cacacgcgtc tgggccgggg tcgtgtgcgc gggcgtgatg atgctcctcg tcgcacaatt
     1021 gcatcgactg ctggtcggcg atgcgtcgtg gctgtcggta tcgatgacgt tcgtgatggt
     1081 cgccgtcgtg ttggggtacc tggctgcacc gctcgtgtcc ggccgctgga gcgaggccgc
     1141 catgcgatcc gcattgcggc tcacgatgac gccctgggca atccatgccg ccgtgaaagc
     1201 agagcatgac ggccggcgcg gcatctgacg atcgtctcaa gcgcctgcgt tggcgttgtc
     1261 ggcgcggcac gcgcgaactg gatgcgctgt tgggcggctg gctcgatgcg cggcgcgatt
     1321 cgatcgatgc gggcgagctc gcgacgttcg acgcgctgct ggaccagcag gatcccgtgt
     1381 tgtgggattg gctgatgggg cacgccgagc cgccgcgcgc ggattggcgt gcaatcgtgg
     1441 ccgagatacg cgagcgcgcg ggactgccgc aatgaagtcg gcacctgccg tcggcttcga
     1501 ctaccacgcc tcgcgctcgc tcatcctcgc gacgttggtc gtggcattct tcgccgtcgc
     1561 ggcggtgtgg ctgagcggct tgccgtggtt ggcgcagctt gcattgacgc tggggattgt
     1621 tgccgccgcg ggcgtcgcgt tcgggaacct ggtgcgcccg cgcgtgcgtt cgatgcggtg
     1681 gcgaagcgac ggcggcgccg acatttgcct cggcgacgat cggcgcgaag cgcagggcga
     1741 agtctccgcg gcgcgcgtga tgggaccgct gatcgccttg agcctgcgct ggccgccgcg
     1801 cggccgcgcc acgttgtggc tgctgccgga caacctcgat cccgagaccc gccggcgctt
     1861 gcggatgcgc ctgggcgccg tagctgccca ccccctgtca gggaacaccg acaacgcttg
     1921 acggtctgac ccgcttgccc gtgcccgcgc ctcccgccac gctgcgttac gcgggggcgc
     1981 atcggtgttg gcaaccccgc tacgattgcc gctccccatt tccctgttcc cgcttttcgg
     2041 catatcccgc atgtttcgac cgctcgaact ctatatcggc acccgctaca tccgcgccaa
     2101 gcggcgcaac catttcattt ccttcatcag catcgtgtcg atgctggggc tcgtgattgg
     2161 catcaccgcg ctgatcaccg tgatctcggt gatgaacggt ttcgacgggg agcttcgctc
     2221 gcggatcctc gggatggttt cgcaggcgac catcagcggt gtcggcaatg acatgcccaa
     2281 ctggcagcag gcgctggcca tcgctgagca gaatcctcat gtccgcggcg ccgcgccgta
     2341 cgtggagcag tatgggctgc ttcgcggcaa cgctgcgaac cagggagcgg tcattcgcgg
     2401 catcctgccg gatcaggaac cgaaggtcgc cgacatcggt tccaagatga tcgccggcaa
     2461 gctgtcttca ttgaccgatg gctcttgggg catcgtgctg ggccgggacc tcgcgctggc
     2521 gctcggcgtg cgtcccggcg acaagatcgt cgtgtacgca accgaagtca ctgcaaatcc
     2581 gatcaccggc gtggttccgc gcctcaagcg atttacggta accggcatct tcgaagcggg
     2641 gatgcaggaa tacgattccg ggctcgcgat agtcaacctg cacgacgccg aaaagcttta
     2701 ccggatggat gggccgacgg gcatccgcct gaagctggac gacctgttcg ccgccggtcg
     2761 ggtggcccag aacctgcagg accagttcgg cgaaatgggc gccgcgtacc gtgcgcaggg
     2821 ctggatgcag agccacgcca atttcttcaa ggcgatcgcg atggagaaga tcgtcatgtt
     2881 catcatcctg tcgctgatcg tggcggtcgc ggccttcaac ctggtgtcgt cgctggtgat
     2941 gctcgtcacc gacaagcagt cggatatcgc gatcctgcgc accctcggtg cgacgccgcg
     3001 cagcatcatg ggcgtcttca tgatccaggg gatgctgatc gggatcatgg gcatcgtggt
     3061 cggcgtggtg tgcggcgtgg cgctggcgat caacctgccg tcgatcgtgg actggatcca
     3121 gaacatcacg ggctaccagt tcctgtccga gtcggtgtac tacatcaaca acgttccgag
     3181 ccagttgcac tggggcgatg tcggctggat caccggcatg gcgttcctgt tctcgctgct
     3241 ggcgacgatc tatcccgcgt ggcgcgcgtc gcgcacccaa ccggcgcagg cgttgcggta
     3301 tgaatagcag ggagcaggga gcagggagca gagggctggg caacggcgtg gtcctgcggg
     3361 cgagccatat cgccaagacc tacaaggaag gttcgctgcg cacgccggtg ctggaggacg
     3421 cgagcttcga gctggcacgc ggcgagacgc tggcgatcgt cggtgcatcg ggttcgggca
     3481 agagcacgct gctgcacatc gtcggcggcc tcgacacgcc gacgaagggc atgatcgagc
     3541 tggaagggcg cgagttgtcg aagctgccgg acgccgaacg cggccgggtg cgcaaccgtt
     3601 cgcttggttt cgtctatcag ttccatcatt tgttgcccga gttcagtgcg ctggaaaacg
     3661 tcgcgatgcc gctgctgatc cgcggcacgc cgatctccga tgcgcgcgag caggcgacga
     3721 cgctgctgga acgcgtgggg ctcggccatc gcctcgaaca caagccgggc gaattgtctg
     3781 gcggcgaacg ccagcgctgt gccatggcgc gggcgctggt gacgcggccc tcgtgcgtgc
     3841 tggccgacga gccgacgggc aatctcgacg aacagaatgc cgcttccctg tacgagctga
     3901 tgctggagtt gaaccgcgag atcggcacca gcatcgtgct ggtcacccac gaccggaagc
     3961 ttgcggctca aatggatcgc gtgctggaac tgaccggcgg cgcattgcac ccgctgcccg
     4021 acgactgaat ccgtgatgga cacgcgtcgc agggaacgcc gcgtcgtccg gaccaccccg
     4081 gggatccttg ccggcgcgat ggcgttcctt gcaggcgccg tgctggtgca ggcgctgccg
     4141 gtcctgccgc cgcgctggct ggacgccgta tggggctgcg cagcactcgc cgggctggga
     4201 ttgtgcctgc gaaagcgcct gcctggcggc gtgctattcg ttgcaatcct gctcggggcc
     4261 ttcgcgtgga cggcgtggcg cgccgacctc gcgctgggtg cccggctgcc gcacgcgctc
     4321 gaaaagcagg acatcctcgt gacagggcgc gtcaccgact tgcccgagcc gcgggaagat
     4381 tccacccgct tcgatttcga aatttcgaac gcgcatttcg acggacgccc gatcgccgtc
     4441 cgcggccatg tgcgcctgtc ctggtatgca acgcgcggcc atgcgccacc gacgatcgca
     4501 ccgtgctcga cctggcgcct gcgggtgcgg atgaaacgtc cgcatggcct ggtcaatccg
     4561 ggtggaatgg atttcgagcg ctcggcgctc cagaagggca tcgtcgcgac cggctacgtg
     4621 cgcgaggatc ccgcgaacgc aatgctcgac gccggggcat gcgtcgacgg ggtgcgcgca
     4681 cgcatcggcg gcgccatcga tgccgcgctg ccgaacgacg cgcatgcggc acgcttgctg
     4741 cgtgccttga gcgtcggcga cgagcgtgcg ctcgacgagc acgactggca ggtggtgcgc
     4801 accaccggca tcagccacct gatcgcgatt tccggcttcc acgttggact cgccgccgta
     4861 ttcggcgcgc tgctggtgcg gctcctgtgg tggctgttcc ccgccttggc gctgcgattg
     4921 gcgcggcctt tggcggaagc cacggtggcg ttccccgcgg cgcttgccta cggcgcgctg
     4981 gcgggcttcg gactgccgac cacccgcacc ctgctgatga tcgcggtcgt cgcgggttgg
     5041 cgtctgctgc gccgcggcgg cggcttcggg gaaggattcg gcctcgcgct ggccgccatt
     5101 ctggtgctcg atccgctgtc ggtgctgtcg gccggcttct ggctgtcgtt cgcgggcgtc
     5161 gcgctgctcg cgtggacgct ggcgcgcgat tccggctggc gcggccacct caaggaaatc
     5221 ggttttgcac cgctgctgat gacactggct ctgctgccgc tgacggtgtg gtttttcggg
     5281 caggcatctc tggtcgggcc gatcgcgaac ctcgttgcgg tgccgttcgt cagcttcgtg
     5341 atcgtgccga tcgatctcgc ggcgtgcgcg ctgctgttcg cgtggccagc ggccggccag
     5401 ggattgctgc acgtttgcgc gcacctcgtc gacggattgt ggtggttcct ggtccacctc
     5461 gccgcgtgga agcccgcgat gcagtacctg ccacagccga cgttcctggc gttcgccctc
     5521 gcgtgcatcg gcgccgtgtg gttgctggcg ccgcggggcg tgccggcgcg cgccctcggc
     5581 gcgctgttgc tgctgccctt gtgctggccg cgcgccagcc tgccgtcggc cggcgccttc
     5641 cgcgccaccg tggtcgacgt ggggcagggc ctctcggtgc tggtgcgcac ccgtgggcat
     5701 gcattgctgt tcgacaccgg cgcgcgctat ccctccggct ttgacctggg tgccgcggcg
     5761 gtggtgccga gcttgcacgg cctcggtgtc acgcacctgg accggctgat catcagccac
     5821 ggcgacaacg accacgcggg cggcgcggcg tcggtgctgg ccgcgtatcc gggtacgccg
     5881 gtatggggcg gcgaacccgc gcgcggtccg gtgccgatgc ggcaatgcca tgcggggcag
     5941 cattggcagt gggacggcgt cgctttccgg atgttgcggc cgcctgcgcc ggtcgcgatc
     6001 gatggcaacg acgcgggctg cgtattgctg gtgagcggca aggacgggcg cctgctgctg
     6061 cccgcggaca cctcgtcgaa ggtcgagccg gacatcgccc gtgcggtccc gccggggccg
     6121 ccgctggtgc tggtggtacc gcaccacggc agcaagacgt cttcgagccg cgcctatctc
     6181 gaggcgttgg cgccacgctt cgccattgcg tcgaccggct atttgaatgg ctaccaccac
     6241 cctgcgccgg tcgtcgccgc acgctacgcg aaactgggga tcccgctttt gaatacgccg
     6301 gcgaccggcg cggtgcgtat cgcctttccg gcaaatgcac cgccgcggat cctttccgaa
     6361 gcacgcatca gacaggcgca ttattggcgc gagcattgat gcggtattgc gcggcggggt
     6421 gccttcggtc gggctttctc ccggacaggt ccgggatcaa cccaacctac gcaagccgcc
     6481 atcgtacgtt ggcggtgggc gcagcgaagc ccaacaggcg cgccggttcg tcgcgcgcca
     6541 gcgttgtcgc gtcctctccg tgtcgattgt tgggcttctc ccggacgagt ccgggatcaa
     6601 cccagcctac gccagccgcc ctcgtaggtt ggtgctgagc gcagcgaagc ccaacaggcg
     6661 cgccggttca tcgcgcgcca gcgttgtcgc ggcccctccg tgtcgattgt tgggcttctc
     6721 ccggacaagt gcgggatcaa ccccaccttc gcaagccgct ttcgcaggtt ggtgctgagc
     6781 gcagcgaagc ccaacgatcg ccgctgcatc ggccgcccgg gcagccggaa cagcctgcca
     6841 gagtgcgacc gcggccacgg tcgtacgggc cggcgctggt atgatgcgcg tgcatcgcgg
     6901 agcacccgca gcggaggttg caagtgtttg aaatcctgaa ggcgggcggc tggggcatgc
     6961 tgcccatcct ggtgtgctcg gcggtcgggc ttgcgatcgt cctggagcgt ttctggaccc
     7021 tgcgccgcgg cacggtgctg ccgccgggcc tcggcgacca ggtgcgaagc tgggcgcatt
     7081 cgcagcaatt gacctcgtcc cacatccagg ccctgcggga aaattccccg ctgggcgaac
     7141 tgctggccgc cgcgctcgac gtgcgcaacc gctcgcgctc ggagatcaag gaacggatcg
     7201 aggacaccgg gcggcacgtg gtgcacgatc tcgagcgcta cctcaacacc ctcggcacga
     7261 tcgccctgat cgggccgctc ctcggcctgc tcggaaccgt gttcggcctg atccggatgt
     7321 tcctggcggt gatggtgtc
//
LOCUS       DASSNH010000170         9257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_511798, whole genome shotgun sequence.
ACCESSION   DASSNH010000170 DASSNH010000000
VERSION     DASSNH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511798"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFK00_06365"
     CDS             complement(<1..725)
                     /locus_tag="VFK00_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592613.1"
                     /translation="MALISVTPPAAEPIDLAEAKVHLRYIDTAQDALVAAAIAAVRGQ
                     VEGLTRKQLVAARWKQVLDAFPYNGNPFEFGRSYSRPANAIYLERGPLLQVVSIQYLD
                     MAGVLQTVDPSIYTIDSASDPVRITPVFGQIWPIPMPQIGAVSVTFDAGFAAPITANG
                     NNVTVRGWLPLNIGDVVRFTNSGGALPAPLQPNVDYFVQSVVGPGIYTFSATAGGAAI
                     ALTNAGTGTSFLGAVPEGIKSWM"
     gene            complement(777..1223)
                     /locus_tag="VFK00_06370"
     CDS             complement(777..1223)
                     /locus_tag="VFK00_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592614.1"
                     /translation="MYAQNVKASEQIGVLAAINPSSQAAGAANSGWVSVADFQKLLAI
                     VQVGAFGASATVDAKIQQATDSAGTGAKDVAGAAIAQMLAAGGNNVQAEVNLDAAQLD
                     VEGGFGFVQLSVTVGTAATETAALLLGFNPRFAPPANATSVAQVVG"
     gene            complement(1289..2584)
                     /locus_tag="VFK00_06375"
     CDS             complement(1289..2584)
                     /locus_tag="VFK00_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HET7592615.1"
                     /translation="MSKKLRELQARKAKHVGAMRAISDAAAAENRDLTDEESASFEAE
                     KKSLEGVNAAIQREQDLIEQERSIGAVEIGDQASVSGGAPRAEQDPRRGFQSFGEFAA
                     AVRQGSSRNGAIDERLTIGAAVPGTYANEGAGADGGFLVPPEFSTQIFTLSLDDDALL
                     PLTDNTNVNGNGMVFPKDETTPWGTDGVRAYWQAEASQAVATKPKLSTSAMRLHKLMA
                     LAPVTDELLSDASALDSYLPGLMARSIRWKTNEAILFGDGAGKPLGVFEGGAAVVQAK
                     DGSQATKTVTLSNITNMIARLPPGSFPRARWMITPDALPALFGLTLGNYPIYLPINSG
                     AQGSPYGTLMGRPIIVSQHAAAFSSQGDVSLVDLQYYRTITKRGGIQTATSMHLYFDA
                     DAVAFRTTFRVDGQPKIVNPITQAKGSNTLSPFIQLASR"
     gene            complement(2702..3592)
                     /locus_tag="VFK00_06380"
     CDS             complement(2702..3592)
                     /locus_tag="VFK00_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET7592616.1"
                     /translation="MKHERFIAWALSQPWALMPERITAYAAVLARQIAPSAMEDNEPI
                     VAGPSRSREKPRAGSIAVIPVYGPIVQRASQIDICEGGTSTQSISNALADAMADDSIS
                     QVLLDIDSPGGSVYGVQEVAAEIARAKKPVVAIANSLAASAAYWIGSAASELYVTPGG
                     EVGSIGVWTAHQDISKALEAEGINVTLISAGPFKVEGNPYQPLGADAQKFMQSRIDDY
                     YGAFTRAVAKGRKVSVDQVRNGMGQGRVLGADAAKAEGMVDGIATFEEVIANMQRTHA
                     AATPRANRLAAAQRDIAILG"
     gene            complement(3589..5073)
                     /locus_tag="VFK00_06385"
     CDS             complement(3589..5073)
                     /locus_tag="VFK00_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET7592617.1"
                     /translation="MFLSRFRASEDRSPWGDWWFEPVGMRTATGLHVTPDRALQLPVV
                     LACVRVLAESFSILPLRVYRNTGSSRKRLTKHWLLDLIGRTPNPFQTAFEWREMMMGH
                     LALRGNAYNQIIADRRGNITALMPLHPDRVKIEFIGTGQFDYRYRYAQRDGTDMVLTR
                     GEVWHIRGLSSDGVMGLSPIALARESAALGLAAQDYGARFFANDAKPGGGWIEFPGNF
                     KDKAARENFRESWQEAQSGLNRGKIAVLEYGMKFNELGMTNKDSQFLEARQFQVSDIA
                     RIFRVPPHLVGDLSKATFSNIEQQSLDFVIHTMTPWAERWEASAETSLLTEDEADIEA
                     EFDFTGLLRGDQAARTAYYQGGIQSGWMVRNEAREREGMEPLPGLDEPLRPLNMVEES
                     EAGNGQQSASPAQPLPGNDDDTPAAPDEPADDSADARLNALLATNAARMARRLAGGSA
                     VGADVLAEALAVPVDRAAAWLENRNAAASEPECMAALLELGAAA"
     gene            complement(5073..5249)
                     /locus_tag="VFK00_06390"
     CDS             complement(5073..5249)
                     /locus_tag="VFK00_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592618.1"
                     /translation="MNVAVFDIALALGWAMVTAGALLANIPCGLIVGGVLLLGLTFAS
                     AWLGGVYARRKDDG"
     gene            complement(5246..6688)
                     /locus_tag="VFK00_06395"
     CDS             complement(5246..6688)
                     /locus_tag="VFK00_06395"
                     /inference="COORDINATES: protein motif:HMM:NF042863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HET7592619.1"
                     /translation="MFFVVQLFGFRNADDTRRFTSALLALARKNAKSTLAAGILLYCQ
                     CCEDETGPQIVSAATTGSQARIVFNVAKRMVEKTPDLQAAFDVHPFANAIASSGNGGS
                     FKPINSKASTQDGLNPSATALDEVHAHKTSELLDVLQSAAGARRNPLWLYTTTEGYET
                     PGPWPEIRRFAQQLLEGVVEADHFLALLYALDEDDDDFDEPKFIKANPLLDASPILQR
                     ELKKAAAEAKAMPGRLAEFRIKRLNRQASAANGWIDLPKWKRCAGAVDLDALVGVRCI
                     AGLDLASTTDLAAWRLIWLLEDGTVATWGRRWAPEAAVDRRKLRGTVPYEAWRGAGLI
                     EVTEGDVIDYAVIEKRIREDCERFHVEQIAFDRWNATDLVNRLSADDFPLIEFIQGPK
                     SYHPAMQELERLYLTRRLAHGGDPVLQWCASNLVVRYDANMNMAPDKKRSPEKIDDMT
                     ALLMAIGVSMAQADDAPVTLGSDYSLAVVG"
     gene            complement(6933..7358)
                     /locus_tag="VFK00_06400"
     CDS             complement(6933..7358)
                     /locus_tag="VFK00_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592620.1"
                     /translation="MARRGPKPQSPAMQAAKGTVQPCRAVTVLFPDHASRPDPEAIDP
                     PKWLNAAAKRIWAAKVERYRQRSQKVGGFEDALAQYCALEAEIIANRRKGFPVPMAAI
                     NAHRIWAAEFYDTPASQVAKPGAVGAGGNKFAQFGKRPA"
     gene            complement(7810..8025)
                     /locus_tag="VFK00_06405"
     CDS             complement(7810..8025)
                     /locus_tag="VFK00_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592621.1"
                     /translation="MDARLIIIGALALACSACAAPQVRTVTDYCTPWRPIYVSRQDVL
                     TDGTARAILEHDRTGAKLCGWTPSKVK"
     gene            complement(8010..8330)
                     /locus_tag="VFK00_06410"
     CDS             complement(8010..8330)
                     /locus_tag="VFK00_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592622.1"
                     /translation="MIALWLAKIWTKAGTWLALAGAVVAAIFAAMLYGEHKGKQVGVS
                     DAAKQAVADARAAQVAVTDAANVRATVEADITKLPEAPAQKVADADLATAAGELRRDG
                     WTRD"
     gene            complement(8327..8587)
                     /locus_tag="VFK00_06415"
     CDS             complement(8327..8587)
                     /locus_tag="VFK00_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592623.1"
                     /translation="MKFVPDARNWWKWHTTYVFGVLGVFPVVWLNSPDLQALLPPSVV
                     SKIAPFVAALGLFLRLRDQMLKVPLPKPTVPPSNDFHQGKDS"
     gene            complement(8584..9090)
                     /locus_tag="VFK00_06420"
     CDS             complement(8584..9090)
                     /locus_tag="VFK00_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592624.1"
                     /translation="MSLLPLSISPADALDKIIVPALATLPTETDSPVARLMLLAIALQ
                     ESGLRSREQTGGPARGLWQFERNGVLAVMHHTRTADVVFHWCEEKDVTYGSNSIYERL
                     ALDDELACAFARLLLWTDPRPLPDIGDCMSAWNLYERCWRPGKPSYTRWRDTAYPQAL
                     QTIKGQSA"
BASE COUNT         1501 a         3089 c         2993 g         1674 t
ORIGIN      
        1 ttcatccacg acttgatgcc ctccgggacc gcgcccagga agctggtgcc ggtgccggcg
       61 ttggtgagcg cgatcgcggc gcccccagca gtcgcggaaa atgtgtagat gccgggtccg
      121 accaccgact gcacgaaata atcgacgttg ggctgcaatg gcgccggcaa cgcgccgccg
      181 ctgttcgtga accgcaccac gtcgccgatg ttgagcggca gccagcccct cacggtcacg
      241 ttgttgccgt tcgcggtgat gggtgctgcg aacccggcat cgaatgtcac cgagaccgcg
      301 ccgatctgcg gcatcggtat cggccagatc tggccgaaca ccggcgtgat ccgcaccggg
      361 tccgacgcgg agtcgatggt gtagatcgat gggtcgaccg tctgcaggac gccggccatg
      421 tccaggtatt ggatcgagac cacctgcagc aacggtccgc gttccaggta gatcgcgttc
      481 gccgggcggc tgtaggagcg cccgaactcg aaaggattgc cgttgtacgg gaacgcgtcg
      541 agcacctgct tccagcgcgc ggcgaccagc tgcttgcgcg tgagcccttc gacctggccg
      601 cgcacggccg caatggcggc agccacaagc gcgtcctgcg cagtgtcgat gtagcgcagg
      661 tgcaccttgg cctcggcgag gtcgatcggc tcggccgccg gcggcgtgac ggaaatgagt
      721 gccatggatt cgaaccagaa ggcccggcgg ccaggagtac ccgccgggcg ttccgatcag
      781 cccacgacct gcgcgacgct ggtggcgttc gcgggaggtg cgaaccgcgg gttgaagccg
      841 agcaacagcg cagcggtttc cgtggccgcg gtaccgacgg tcaccgacaa ctgcacgaag
      901 ccgaaaccac cttcgacgtc gagctgcgcg gcgtcgaggt tgacctcggc ctgcacgttg
      961 ttgccgccgg cggccagcat ctgcgcgatc gcggcgcccg cgacatcctt cgcgccggtg
     1021 ccggcgctgt cggtggcctg ctggatcttc gcgtccacgg tcgcgctcgc gccgaacgcg
     1081 ccgacttgca cgatggcgag caacttctgg aagtcggcca ccgagaccca gccgctgttc
     1141 gcggcgccag ccgcctggct ggacgggttg atggccgcca ggacgccgat ctgctcggac
     1201 gccttcacgt tttgtgcgta catggtgaat tcctcttgga aagggttgga gaaagcaccg
     1261 ccccgaaggg cggcgtttca gtgccggatc agcgcgatgc caactggatg aacggggaca
     1321 gggtgttgga acccttggcc tgcgtgatcg ggttgacgat cttcggctgg ccatccacgc
     1381 ggaacgtcgt gcggaacgcc acggcgtccg catcgaagta caggtgcatg gaagtcgccg
     1441 tctggatacc gccgcgcttg gtgatggtgc ggtaatactg cagatcgacc agcgaaacgt
     1501 caccctgcga gctgaacgcc gcggcatgct ggctcacgat gatcggacgg cccatcagcg
     1561 tgccgtaggg cgaaccctgc gcgccgctgt tgatgggcag gtagatcggg tagttaccga
     1621 gcgtcaggcc gaacagcgcg gggagcgcat ccggcgtgat catccagcgc gcccgcggga
     1681 agctgcccgg aggcagccgc gcgatcatgt tggtgatgtt ggagagcgtc acggtcttgg
     1741 tggcctggct tccatccttg gcctgcacca ccgccgcgcc gccctcgaac acgccaagcg
     1801 gcttgccggc gccatcgccg aacaggatcg cttcgttggt cttccagcgg atcgagcgcg
     1861 ccatcaggcc cggcaggtac gaatcgagcg cggaagcatc ggacagcagc tcgtcggtga
     1921 ccggcgccag tgccatcagc ttgtgcagcc gcattgcgga cgtgctcaac ttcggcttgg
     1981 tggccacggc ctgggaggct tccgcctgcc agtacgcgcg cacgccgtcg gtgccccagg
     2041 gcgtcgtttc gtccttcggg aacaccatgc cgttgccatt gacgttggtg ttgtcggtca
     2101 acggcaacag cgcgtcgtcg tccagcgaca gcgtgaagat ctgcgtgctg aactccgggg
     2161 gcaccaggaa gccgccgtcg gcaccggcgc cctcgttggc gtacgtgccc ggcacggctg
     2221 cgccgatggt caggcgttcg tcaatcgcgc cattgcgtga gctgccctgg cgcacggccg
     2281 cggcgaactc gccgaagctc tggaagccgc ggcgcggatc ctgctcggcc cgcggcgcac
     2341 cgccgctgac actggcctgg tcgccgatct cgaccgcacc gatgctgcgt tcctgctcga
     2401 tcaggtcctg ttcgcgctgg attgcggcgt tgacgccctc gagagacttc ttctcggctt
     2461 cgaacgatgc cgattcctcg tcggtgaggt cgcggttttc cgccgcggcg gcatcggaaa
     2521 tggcgcgcat ggcgccgacg tgcttggcct tgcgagcctg cagctcgcgg agcttcttgg
     2581 acatgggaat gttcctcggg ttggatacga aaaaaccgcc acgcgggcgg tttgctttgt
     2641 gccggctgcg gccgtcgggc cgcgccgacg cgccatcggg cgcgccgggt cgggttggca
     2701 ctcaacccag aatggcgatg tcgcgttgtg ccgcggctag acgattggcg cgcggcgttg
     2761 ccgcagcatg ggtgcgctgc atgttggcaa tgacttcctc gaaggtggcg atgccgtcga
     2821 ccatgccttc ggccttggcg gcgtcggcgc cgagcacgcg gccctggccc ataccgttgc
     2881 gcacctggtc gacgctgact ttgcgaccct tggccacggc gcgggtgaac gcaccgtagt
     2941 agtcatcgat gcgcgactgc atgaacttct gcgcatcggc gccgagcggc tggtacgggt
     3001 tgccctcgac cttgaaggga cccgccgaaa tcagcgtgac attgatgcct tcggcctcga
     3061 gcgccttgct gatgtcctgg tgcgcggtcc agacaccgat cgagccgact tcgccgcccg
     3121 gtgtgacata gagctcgctc gccgccgagc cgatccagta cgccgcactg gccgcaaggc
     3181 tgttggcgat cgccaccacc ggctttttgg cgcgtgcgat ttccgccgca acttcctgca
     3241 cgccgtacac cgaaccgccg ggcgagtcga tgtccagcag gacctgcgag atactgtcat
     3301 cggccatggc atcggccagc gcattgctga tcgactgcgt ggatgtgccg ccttcgcaga
     3361 tgtcgatctg gctggcacgc tggacgatcg gcccgtacac gggaatgacc gcgatcgagc
     3421 ctgcgcgcgg cttttcgcgc gaacgcgagg gaccggcgac gatgggttcg ttgtcttcca
     3481 tggcagacgg cgcgatctgg cgcgccagca ctgcggcata cgcggtgatc cgctccggca
     3541 tcagcgccca tggctgcgac aaggcccatg cgatgaagcg ttcgtgtttc atgctgcggc
     3601 tcccagttcg agcaatgcgg ccatgcattc gggttcggat gcagccgcat tgcggttttc
     3661 cagccatgcc gcggcgcggt cgacgggcac ggctagcgcc tcggcgagca catcggcgcc
     3721 cactgcgctg ccgccggcga ggcggcgcgc catgcgcgcg gcattggtcg ccagcagcgc
     3781 gttcaggcgg gcgtccgcgg aatcgtcggc aggttcgtcc ggcgcggctg gcgtgtcatc
     3841 gtcattcccg ggcagcggct gtgccggcga tgcggactgc tggccgttgc cggcttccga
     3901 ttcctcaacc atgttcaacg gccgcaacgg ctcgtcgaga ccgggcagcg gctccatacc
     3961 ctcgcgctcg cgcgcttcgt tgcgcaccat ccatcccgac tggatgccgc cttggtagta
     4021 cgcggtgcgt gcagcctgat cgccgcgcag cagccccgtg aagtcgaatt cggcctcgat
     4081 gtcggcctca tcctcggtca gcagcgacgt ttccgcggac gcttcccaac gctccgccca
     4141 cggcgtcatg gtgtggatca cgaagtcgag cgactgctgc tcgatgttcg agaatgtggc
     4201 tttggacagg tcgccgacca gatgcggcgg cacccggaag atgcgcgcga tgtcggacac
     4261 ctggaactgg cgcgcctcga ggaactggct gtccttgttg gtcatgccga gttcgttgaa
     4321 cttcatgccg tactcgagca ccgcgatctt gccgcggttg agcccggact gcgcttcctg
     4381 ccacgactcg cggaagttct cgcgcgccgc cttgtccttg aaattgccgg ggaactcgat
     4441 ccagccgccg ccgggtttcg catcgttcgc gaaaaaccgg gcgccgtaat cctgcgcggc
     4501 caacccgagc gccgccgatt cgcgcgccag ggcgatcggc gagagcccca tgacgccatc
     4561 gctggaaagc ccgcggatgt gccatacctc gccgcgggtc agcaccatgt cggtaccgtc
     4621 acgctgcgca tagcggtacc ggtaatcgaa ctggccggtg ccgatgaatt caatcttgac
     4681 gcgatccggg tgcagtggca tcagcgccgt gatgttgcca cgacgatcgg cgatgatctg
     4741 gttgtaggcg ttcccgcgca gcgccaggtg gcccatcatc atttcgcgcc actcgaacgc
     4801 cgtctggaac gggttcggag tgcggccgat caggtcaagc agccagtgtt tggtgagccg
     4861 cttccggctc gaaccggtat tgcggtacac ccgcagcggc aggatgctga acgactcggc
     4921 cagcacccga acgcaagcca gcaccaccgg cagctgcagc gcccggtccg gcgtgacatg
     4981 caggccggtg gccgtgcgca tgccgactgg ctcgaaccac cagtcacccc atggcgagcg
     5041 gtcctcgctg gcgcggaaac gcgacaggaa catcagccgt catccttccg gcgcgcgtac
     5101 acgccgccga gccatgcgct ggcgaacgtc aggcccagca gcagcacgcc gcccacgatt
     5161 aggccgcacg ggatgttggc cagcagcgcg ccggccgtca ccatcgccca gccgagtgcc
     5221 agcgcgatat cgaacaccgc aacgttcatc cgaccaccgc aagcgagtaa tcagagccga
     5281 gcgtcaccgg agcgtcatcc gcttgcgcca tgctgacccc gattgccatc aagagcgctg
     5341 tcatgtcgtc gatcttctcc ggtgagcgtt tcttgtcggg cgccatattc atgttcgcgt
     5401 cgtaacgcac cacgaggttc gatgcgcacc actgcaatac cggatcgccg ccgtgcgcca
     5461 gccggcgggt gaggtaaagc cgttccaatt cctgcatggc agggtggtac gacttcggcc
     5521 cctgtatgaa ttcgatcagc ggaaaatcgt ccgcagacag acggttcacg agatcggtcg
     5581 cgttccacct gtcgaatgcg atctgttcca cgtggaagcg ttcgcagtct tcgcggatcc
     5641 gcttttcgat caccgcgtag tcgatcacgt cgccttcggt gacttcgatc agtcccgcac
     5701 cgcgccatgc ctcgtacggc acggtgccac gcagcttgcg ccggtcaacg gccgcttccg
     5761 gtgcccagcg ccgcccccat gtcgcgacgg tgccgtcttc cagcagccag atcagccgcc
     5821 aggccgcaag gtccgtggtg ctggcaaggt cgaggccggc aatgcagcgc acgcccacca
     5881 gcgcatcgag gtcgaccgcc ccggcgcacc gcttccattt cggcaggtcg atccagccgt
     5941 tcgcagccga agcctgccgg ttcaaccgct tgatgcggaa ttcggccagc ctgcccggca
     6001 tagccttggc ttccgccgcg gcctttttca gctcccgctg caggatcgga gatgcatcca
     6061 gcagcgggtt ggccttgatg aacttcggct cgtcgaaatc gtcgtcgtct tcgtccagtg
     6121 cgtacagcag cgccaggaag tgatcggctt cgaccacgcc ctcgagcagc tgctgcgcga
     6181 accgccggat ctccggccac gggcccggcg tctcgtatcc ctccgtcgtg gtatagagcc
     6241 acaacgggtt gcgccgcgcg cccgccgcgg actgcagcac gtccagcagc tcgctcgtct
     6301 tgtgcgcgtg cacctcgtcg agggccgtcg cgctagggtt caggccgtcc tgcgtcgatg
     6361 ccttggaatt gatcggcttg aagctgccac cgttcccgga actcgcgatc gcgttggcga
     6421 atggatgaac atcgaaagcg gcctgcaggt caggtgtctt ttcgaccatc cgcttggcga
     6481 cgttgaagac tatccgcgcc tggctgcccg tcgtggccgc gctgacgatc tgcggccccg
     6541 tttcatcctc gcagcactgg caatacagca ggatcccggc cgcaagcgtc gatttcgcgt
     6601 tcttgcgcgc cagcgccaac agcgccgacg tgaaccgcct cgtatcgtcg gcgttccgga
     6661 acccgaacaa ctggaccacg aagaacacct ggaacgggtc caggacgatc gtgggcgagt
     6721 cccacttccc ctctacgtgc ggcaggctct cgatgaaccc gcaggcatcg caggcgtgcc
     6781 aggcgtcgaa gtggaacgac gcgcgcctgg ttccggcacg tttgagatct gccagaaaac
     6841 gccttgcggc cagccgagtc catttgcaat gccgaacgcg atggcgatcg gcaactgctt
     6901 catgcgcata cgttgtggcg atcgcgacgt aatcacgccg gccgctttcc gaactgggcg
     6961 aacttgttgc cgccggcgcc aacggcgccc ggcttggcga cttgcgatgc tggcgtgtca
     7021 tagaactctg cggcccagat gcggtgcgcg ttgatcgccg ccatcggaac cggaaagccc
     7081 ttccggcggt tggcgatgat ttcggcctca agcgcgcaat actgggccaa ggcatcctcg
     7141 aacccgccga ccttctgcga ccgctgccgg tagcgctcga ccttggcggc ccagatgcgc
     7201 tttgcggcgg cgttcagcca cttcggaggg tcgatcgcct ccgggtccgg ccggctcgca
     7261 tggtcgggaa acaacaccgt caccgcccga cacggctgga cggtcccttt cgcggcctgc
     7321 atcgccggac tttgcggttt gggacccctt ctcgccatga aattctcgga aaatcaactt
     7381 gcatgtgcaa aaaaacgatt gggcagccgg tctagcagac acgcccggcc agagatttga
     7441 tgcccccacc cctatgtatc gactggcgct cgattgcctg cttcgcgccg tcgtgatgcg
     7501 acgtgcacag cgattgccag ttgttgtgat cccagaagcg cgcgcgtgct tgctcgatgg
     7561 ctgccgcatc gccacttgcg agtgcccaag ccgtgcggtg cggcaggatg tggtcgacga
     7621 ctgtcgctgc ggtggtgcgt ccctcttgct cgcacatcac gcacagcggg tgagtgcgca
     7681 accaaccgtc gcgtgcgcgc tgccagcgtg agccgtaccc gcgttgtgcc gacgtgtatg
     7741 cagtacctgg cgctgcgtgg acgcgcgcaa tgcgcggcag cggcttgtgg gttggaacgc
     7801 ggctcggcat cacttcacct tgctcggcgt ccagccgcaa agcttcgcgc cggtgcggtc
     7861 gtgttcgagg atcgctcgtg ccgtgccatc ggtcagcacg tcttgcctgc tgacgtagat
     7921 cggccgccac ggcgtgcagt agtccgtgac cgtgcgcact tgcggcgcag cacatgcgct
     7981 acatgcaagc gcgagtgcac cgatgatgat cagtcgcgcg tccatccgtc ccgtctcaat
     8041 tccccggccg cggtggctag atcagcatcc gcgacctttt gtgctggcgc ttccggtaat
     8101 ttcgtgatat cggcttcgac cgtggcgcgc acgttcgcag cgtcggtcac cgcgacctgc
     8161 gcggcgcgcg catcagcaac ggcctgcttg gccgcatcgg agacgccgac ctgcttaccc
     8221 ttgtgctcgc cgtacagcat cgcggcgaag atcgccgcga ctactgcgcc ggccagcgcc
     8281 agccatgtgc ccgctttcgt ccagatcttc gcgagccaca aggcaatcat gagtctttgc
     8341 cctggtggaa atcgttgctc ggtggaacgg tcggctttgg caacggaacc ttcagcatct
     8401 gatcgcgcag ccgcaaaaac aggcccaacg cagccacgaa cggagcgatc ttgctcacga
     8461 ccgacggcgg cagaagcgcc tgcaggtcag gcgaattcag ccataccact gggaacacgc
     8521 cgagcacgcc gaagacgtac gtcgtgtgcc acttccacca gttgcgcgcg tccgggacga
     8581 atttcatgcc gattggcctt tgatcgtttg cagcgcctgc gggtatgcgg tgtcgcgcca
     8641 gcgggtgtag ctgggcttcc cggggcgcca gcagcgttcg tacaaattcc acgcgctcat
     8701 gcaatcgccg atatccggca gcgggcgcgg gtcagtccac agcagcagcc tggcgaacgc
     8761 gcaggccagt tcatcgtcaa gcgccagccg ttcgtaaatc gagttgctgc cgtaggtgac
     8821 atctttctct tcgcaccagt ggaacaccac gtccgcagtg cgagtgtggt gcatcacggc
     8881 caacacgccg ttgcgttcga attgccatag cccacgggct gggccgcccg tctgctcgcg
     8941 ggagcgcaac ccggattcct gcagcgcgat cgccagcagc atcagccgcg ctacgggact
     9001 gtccgtttcc gtcggcagcg tcgccagcgc aggcacgatg atcttgtcca gcgcatcggc
     9061 aggcgaaatc gacagcggaa gcaggctcat ggcttagcga cgaaccagtg aatcaacgtg
     9121 tacagggccg ccgcgacggt tcccgatatg ccggcaaccc aggcaattgc ccgccacgca
     9181 ccacgggcgg aaatgaccgc cgcgtgtact tcgtccatcg ttgactcgat gcgatcgagt
     9241 cttgaatcga tccgccc
//
LOCUS       DASSNH010000171         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1291460, whole genome shotgun sequence.
ACCESSION   DASSNH010000171 DASSNH010000000
VERSION     DASSNH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291460"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..645
                     /locus_tag="VFK00_06425"
     CDS             214..645
                     /locus_tag="VFK00_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HET7592625.1"
                     /translation="MTLGLLLSMVALLPRPVADQTCLAATVYMEARSQPARGQLAVAE
                     VALDRLDMGRYGDTICEVVTAPHQFAVTTTSPNFRIVNQRAWNKAWQIAGKAIAIWNE
                     PSKDRKLVVPGADHFIRLGSTATWAKNPIATIGDHEFYAVD"
     gene            complement(757..1263)
                     /locus_tag="VFK00_06430"
     CDS             complement(757..1263)
                     /locus_tag="VFK00_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7592626.1"
                     /translation="MWKRISKGRIATRSVLSIACAAIAALLMAGCAPAYSTRPGYGAP
                     PPGYTGGGRQCYQGCGYVRDIREVQLGSNSSGAALGTVIGAVVGGLVGHQVGGGKGKT
                     AATVAGAVGGGFAGHAIGERSGNGDYGWQIVVQLNNGQYATVTQRNAPQVRVGDYVMI
                     RGDNVYRY"
     gene            complement(1292..1981)
                     /locus_tag="VFK00_06435"
     CDS             complement(1292..1981)
                     /locus_tag="VFK00_06435"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7592627.1"
                     /translation="MKSKTRLKKVVPVSLAFAVATALAGCGGQGNPQAQATPANMTTP
                     AATAELPPGDQLATADEGRQPAAAQPQFARVVAVQPITQSTTTSKPRQVCRDEQVAVP
                     ETYKDKNQVGGAVIGGVVGALAGHQVGGGKGKTLATLGGAAAGAYAGHEIQKHHQENN
                     QAKTETRNVCHTVTDKSTSTRTVGYDVTYSLNGQTGHIRLDHKPQVGSGLPVQNGVVV
                     ASGQQAGNTNQ"
     gene            2097..2867
                     /locus_tag="VFK00_06440"
     CDS             2097..2867
                     /locus_tag="VFK00_06440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7592628.1"
                     /translation="MPEPKPSLFDSHAHLDAAEFDPDRPQVMLRARAAGVGECLVPAV
                     SAAGWTKLHELCAADAGLHPAYGLHPCFLPEHSDEDLARLEDWLVGHPAAAVGECGLD
                     SAEPGLERQLRLLRGHFDLAHRFDLPLVLHARQAFAQVIDALRAFGKPLRGVVHSFSG
                     SLEQAHELARLGFSVGIGAPVTYERARRLRRTVASLPIECLLLETDAPDQPSSSHRGE
                     RNEPAYLPEVLECIAALRREDPAEIARATTANARGLFA"
     gene            complement(2877..>3281)
                     /locus_tag="VFK00_06445"
     CDS             complement(2877..>3281)
                     /locus_tag="VFK00_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HET7592629.1"
                     /translation="FRVKVEMIAWCRRRRQPLIVCGSAGGRTDPTQIRVRDLSRTEHD
                     ALLGLVRRKLRKEFGFPSNRDRYFGVPAVYSLQNVGYPQADGRVCGTRPAGSDSMKLD
                     CGGGLGAATHVTGAFGFAAVGRALEMLLKNPG"
BASE COUNT          496 a         1144 c         1068 g          573 t
ORIGIN      
        1 gcggaccccg catcacgtga gacggcgaag aattcgccgg tttgccgaac gtcgttggcg
       61 cattgcgaac ccccgagccc cggcccctcg ccggcgatcg cttccaggtt ccagccaacg
      121 ctccatccgt tgcggcgcag ccctgcgcgc cgtggttaaa gccaggtgcc ggcctgaccc
      181 ggcatgtggt gccttgcaaa ggagaacgac ccgatgacct tgggcctgtt gctcagcatg
      241 gtcgcactac ttccgcggcc ggtcgccgac cagacctgcc tggcggctac cgtttacatg
      301 gaggcacgca gccagcccgc ccgcggccaa ttggcggtcg ccgaggttgc gctggatcgc
      361 ctcgacatgg gccgctacgg cgacaccatc tgcgaggtgg tcaccgcacc ccaccaattc
      421 gcggtgacga ccacgtcacc caacttccgg atcgtcaacc agagggcatg gaacaaggcg
      481 tggcagatcg ccggcaaggc catcgccatc tggaacgagc ccagcaagga ccgaaagctg
      541 gtggtcccgg gcgccgatca tttcatccgg ctgggctcca cggcaacctg ggcgaagaac
      601 ccgatcgcga ccatcggcga ccacgagttc tacgcggtcg attgacccgt tcgcgagtac
      661 ctgccgcaaa aaagagccct ccgaacggag ggctctttgc tggtgcgatc gtccttgatc
      721 gccttcgcgg tcatgggcgc gaccggcacg gaacgatcaa tacctgtaca cgttgtcgcc
      781 gcggatcatt acatagtcgc ccacccgtac ctgcggcgcg ttgcgctggg tgacggtggc
      841 gtactggccg ttgttgagct gcaccacgat ctgccacccg tagtcgccgt tgccgctccg
      901 ttcgccgatc gcgtgacccg cgaagccgcc gccgaccgca ccggcgaccg tcgccgcggt
      961 cttgcccttg ccgccaccga cctggtgccc gaccaggccg cccacgaccg caccgatcac
     1021 ggtgccgagc gcggcgccgc tgctgttgct gccgagctgg acctcgcgga tgtcacgaac
     1081 gtagccgcaa ccctggtaac actgccgacc gccgcccgtg tagccggggg gcggggcgcc
     1141 atagcccggg cgggtgctgt aggccggcgc gcagccggcc atcaacaatg ccgcgatggc
     1201 ggcacatgcg atcgaaagca cgctccgcgt ggcgattcga cccttggata ttcgcttcca
     1261 catggttcca ctccttttgt gtgcgatgaa atcactggtt ggtattgccg gcttgctggc
     1321 cgctggcgac caccactccg ttctgcaccg gcaagccgct gccgacctgc ggcttgtggt
     1381 ccaggcggat atgcccggtc tggccgttca acgaataggt cacgtcgtag ccgaccgtcc
     1441 tggtcgacgt ggacttgtcg gtcacggtgt ggcagacgtt gcgggtttcc gtcttcgcct
     1501 ggttgttttc ctgatggtgc ttctggatct cgtggccggc ataggcaccc gcggccgcgc
     1561 cgccgagcgt ggcgagggtc ttgcccttgc cgcccccgac ctggtgtccc gccagcgcac
     1621 cgacgacgcc gccgatcacc gcgccgccga cctggttctt gtccttgtac gtttcgggga
     1681 ccgcgacctg ctcgtcacgg cagacttggc gcggcttgct cgtggtggtg gactgggtaa
     1741 tcggctggac cgccacgacc cgcgcgaatt gcggctgcgc ggctgcgggc tgccggccct
     1801 cgtcggccgt agcgagctgg tcgccgggcg gcagttcagc cgtcgccgcg ggcgtcgtca
     1861 tgttggcggg cgtggcctgt gcctgtggat tgccttggcc accgcatccg gcgagcgcgg
     1921 tggcaacggc gaacgccagc gaaacgggta ctaccttttt caatcgtgtc ttgctcttca
     1981 tcgtggtctc ccggcaaccg cctcatggcg gtttgattca aacaaccgga caatgaacgg
     2041 cgcgtcaaca tcgctttttc ggttcatctt gcatttcgtc ccccggcagc acattgatgc
     2101 ctgagcccaa acccagcctg ttcgacagcc acgcgcatct ggatgcggcc gaattcgatc
     2161 ccgaccggcc gcaggtcatg ctgcgcgcac gcgcggccgg cgtcggggag tgtctggttc
     2221 ccgccgtgag cgcggccggt tggacgaagc tgcacgaact ttgcgccgcg gacgccgggt
     2281 tgcatccggc atacgggctg catccctgct tcctcccgga gcattcggac gaagacctcg
     2341 cgcggctcga ggactggctc gtgggccatc ctgccgcggc cgtgggcgag tgcgggctgg
     2401 acagtgccga gcccggcctc gagcggcaat tgcgcctgct gcgcggacac ttcgatctgg
     2461 cgcatcgctt cgacctgcca ctcgtgctgc acgcacgcca ggcattcgcg caggtgatcg
     2521 acgcgctgcg ggcgttcggc aagccgctgc gcggggtcgt gcacagtttc tcggggagcc
     2581 tcgagcaggc gcatgaattg gcgcggctcg gattctcggt gggcatcggc gcgccggtga
     2641 cctacgagcg cgcgcggcgg ctgcgacgca cggtcgcctc gctgccgatc gaatgcctgc
     2701 tgctggaaac cgatgcgccg gaccagccct cttccagcca ccggggcgag cgcaacgaac
     2761 cggcgtacct tccggaagta ctcgaatgca tcgcggcgtt gcggcgcgaa gacccggcgg
     2821 aaatcgcgcg cgccacgacc gccaatgcgc gggggctgtt cgcctgattt gctgcctcag
     2881 cccgggtttt tcaggagcat ctcgagcgcg cgtcccaccg cggcgaagcc gaacgcgccg
     2941 gtaacgtggg tcgccgcacc gagtccgccg ccgcaatcca gcttcatcga gtcggagcct
     3001 gccggccgcg taccgcagac ccggccatcc gcctggggat agcccacgtt ctgcagcgaa
     3061 tacacggccg gcaccccgaa atagcggtcc ctgttggaag ggaacccgaa ttccttgcgc
     3121 aacttgcggc gcaccaatcc gagcaacgcg tcgtgttcgg tccgcgagag gtcgcgcacc
     3181 cggatctggg tcggatcggt gcgaccgccg gccgagccgc acacgatcag cggctgcctg
     3241 cgccgtcggc accacgcgat catctcgacc ttcacccgga a
//
LOCUS       DASSNH010000172         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1569250, whole genome shotgun sequence.
ACCESSION   DASSNH010000172 DASSNH010000000
VERSION     DASSNH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569250"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFK00_06450"
     CDS             complement(<1..496)
                     /locus_tag="VFK00_06450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7592630.1"
                     /translation="MDAELPEPSADEAAHSARLAEAIRGEIGTAGPMSFARFMERCLY
                     APGLGYYSAGRRKFGRAGDFVTAPELGALFARCVARAMAPALRACGAEAAWFELGGGS
                     GAFARDCLLELQALDALPKHYWLLEPSADLRERQEEALRAVLPAELFARCAWLDRPPQ
                     HAWSG"
     gene            564..1313
                     /locus_tag="VFK00_06455"
     CDS             564..1313
                     /locus_tag="VFK00_06455"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HET7592631.1"
                     /translation="MMPNTGPTRPVVLVTGAARRIGATIARTLHAAGYDLALHFRRSR
                     AELDALIADLESARANSTLALQADLADAGRMPELIEATITRFARLDALVNNASVFHPT
                     PLGSITAAQFDALFAANARAPLLLAQAAAPHLEASRGCIVNITDIYAEHPLRQHAAYA
                     MSKAALRMATLALARELAPAVRVNAVAPGAILWPEGQAGADRAEVLARIPLARTGTPQ
                     DIAGAVLWLIRDAGYVTGQTLRVDGGRTIEA"
     gene            1395..2063
                     /locus_tag="VFK00_06460"
     CDS             1395..2063
                     /locus_tag="VFK00_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HET7592632.1"
                     /translation="MPASVPTAHAPPPRRIAQHILDLLADVPHSRERPHPRPRQRSEV
                     LARNARRKAFVASSSLALPPGPIGWLTVLPELVAIWKIQAQMVADIAAACGRHTTLTR
                     EQMLYCLFRHAASQAVRDLVVRTGQRWVVQTATTHTIRRVAERIGFKLSERALGSSLA
                     RMLPVAGAVGVGTYAWYDTRQVGRTAIEMFCGEPGKAAIACTEIRAQRVERDRAQDAR
                     NAGL"
BASE COUNT          349 a          845 c          802 g          333 t
ORIGIN      
        1 cgccggacca ggcgtgctgc ggcggacgat ccagccaggc acagcgcgcg aacagctccg
       61 cgggcagcac ggcgcgcaag gcttcttcct ggcgctcgcg gagatccgcc gagggttcca
      121 gcagccaata gtgcttcggc agcgcatcca gcgcctggag ttcgagcagg caatcgcgcg
      181 cgaacgcccc gctgccgccg cccagctcga accatgcggc ttcggcaccg caggctcgca
      241 gcgccggggc catcgcacgg gcgacgcagc gcgcgaacag cgcgccgagt tccggcgcgg
      301 tcacgaaatc accggccctg ccgaacttcc gccggccggc gctgtagtac cccaatcccg
      361 gcgcgtacag gcatcgctcc atgaagcgcg cgaacgacat cggtccggcc gtgccgatct
      421 ccccgcggat cgcttcggcg aggcgcgccg agtgggcggc ttcatcggcg gaaggttcgg
      481 gaagttcggc gtccatgctg gcgatctcgc tgcgtttgcg gcaggctgtg cactgcgcag
      541 catagcgcgt cgccggaatc cgcatgatgc cgaatacggg cccaaccagg ccggtggtgc
      601 tggtcaccgg cgccgcacgc cgcatcggtg cgacgatcgc ccgcaccttg catgcggcgg
      661 gctacgacct ggctttgcac ttccggcgct cgcgcgcgga gctcgacgcg ttgatcgcgg
      721 atctcgaatc cgcgcgcgcg aattccacgc tcgccttgca ggcggacctc gccgatgccg
      781 ggcgcatgcc cgaactgatc gaggcgacga tcacccgctt cgcacgcctg gacgcgctgg
      841 tgaacaatgc ttccgtgttc catccgacgc cgctgggcag catcaccgcc gcgcaattcg
      901 acgccctgtt cgcggccaac gcgcgcgccc cgctgctgct ggcccaggcc gccgcgccgc
      961 atctcgaggc gtcgcgcggt tgcatcgtca acatcaccga catttatgcc gagcacccgt
     1021 tgcgacaaca cgccgcctat gcgatgagca aggcggcgct gcggatggcg accctggcgc
     1081 tggcgcgcga acttgcgccc gcggtgcgcg tgaatgccgt ggcgcccggg gcgatcctgt
     1141 ggcccgaagg gcaagccggt gccgaccgcg cagaggtact cgcacgcatc ccgctggcac
     1201 gaaccggcac gccgcaagac attgccgggg cggtcctgtg gctgatccgc gatgcgggct
     1261 acgtcaccgg ccagacactg cgggtggacg gcggacgcac gatcgaggcc taggcgatgg
     1321 gaacggcacc ggccgacttg aaccaaaccc gcggcggcgg catcttggag gcgttacgcg
     1381 cgccgaggac gccgatgccc gcttccgtac ccaccgccca tgcgccgccg ccccggcgga
     1441 tcgcgcagca catcctcgac ttgctggccg acgtcccgca cagtcgcgaa cgtccgcacc
     1501 cacgcccccg acagcgcagc gaggtgcttg cgcgcaacgc gcgacggaaa gccttcgtgg
     1561 cgtcgtcctc gctcgcattg ccgcccgggc cgatcggctg gctgaccgtg ctgcccgagc
     1621 tggtggcgat ctggaagatc caggcgcaga tggtcgcgga catcgccgcc gcctgcggcc
     1681 ggcacacgac gctgacccgc gagcagatgc tgtattgcct gttccggcac gcggcctcgc
     1741 aggcggtgcg cgacctggtg gtgcgcacgg gacagcgttg ggtcgtgcaa accgccacca
     1801 cccacacgat ccgccgcgtc gccgaacgga tcgggttcaa gctttccgaa cgcgccctgg
     1861 gcagttcgct cgcgcggatg cttcccgttg cgggtgcggt cggggtcggc acctatgcgt
     1921 ggtacgacac gcgacaggtc ggacgcaccg cgatcgagat gttctgcggc gagccgggca
     1981 aggccgccat cgcctgcacc gagatccggg cccagcgggt cgaacgcgac cgcgcccagg
     2041 acgcgcggaa cgcggggttg tagggcggac actgtccgcc agccatcgcg aaactcggtg
     2101 cacaggatgc ggcgggcgat cctcgccccg cgtgcttttc gtaggagcgc gcctgcgcga
     2161 agcacttgct ccttcgtctg cgcgcgatcg ccggtgcgct ccgctgcaac gttcgtcgcg
     2221 cgcgtggcgc gctcctacat tcgcgagggg agacgttcca gcggcggcag cgagggcgcc
     2281 agcttttgcc aacgcgcggc gagcggttcg ccggcaaacg gctcttgca
//
LOCUS       DASSNH010000173         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_383700, whole genome shotgun sequence.
ACCESSION   DASSNH010000173 DASSNH010000000
VERSION     DASSNH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383700"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..159
                     /locus_tag="VFK00_06465"
     tRNA            67..159
                     /locus_tag="VFK00_06465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Ser,seq:gga)
     gene            176..272
                     /gene="ffs"
                     /locus_tag="VFK00_06470"
     ncRNA           176..272
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFK00_06470"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            394..477
                     /gene="ffs"
                     /locus_tag="VFK00_06475"
     ncRNA           394..477
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFK00_06475"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            667..2307
                     /gene="dnaX"
                     /locus_tag="VFK00_06480"
     CDS             667..2307
                     /gene="dnaX"
                     /locus_tag="VFK00_06480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004572793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7592633.1"
                     /translation="MSYQVLARKWRPKQFEELIGQEHVVKALSHALDSGRVHHAYLFT
                     GTRGVGKTTIARIFAKSLNCERGMSAHPCGECAICKAVDAGRFVDLIEIDAASNTGVD
                     DVRDLIESAQYAPAQGRFKVYLIDEVHMLSKSAFNALLKTLEEPPEHVKFLLATTDPQ
                     KLLVTVLSRCLKFGLKRLLPEQIAGQMRMILGQEGVAFDEGAIEALARGADGSLRDGL
                     SLLDQAISHGGGALRADDVRAMLGTIADNDVGLLLRALAAGDGKALMEEADRIAGLAP
                     DFAMVLEQLANALHRVQLRQLVPGFRGDEAEREVLDALAGEVDPEDVQVWYQFAVGGR
                     RDLPFAPDVRTGFEMALLRMLAFRGGAADAGQPARRAAPVTRSQAQPAPTGATRAAAT
                     QPSASAGVADAPQQPAAKAPAPRIDGLADWEAWIQLAELGGPAGQLARHALPKSFQGD
                     VLTLALTPEHMIFCSDALCHQLQEKLGEAIGRPLRVRVVSGEAVAETPAARAAKARSE
                     QQAAAERAMADDPIVQDFQRQLGAEIIPESIRPAGTRA"
     gene            2304..2627
                     /locus_tag="VFK00_06485"
     CDS             2304..2627
                     /locus_tag="VFK00_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7592634.1"
                     /translation="MKGQLGQLMQQAQRMQEELKRAQEEIAKLEVTGSAGGGMVEVTM
                     TGRHEVRRVHIDRKMMADDPEMTEDMLAAAVNDAVNKVAEASQQKLGGVSSGMNLPPG
                     FKLPF"
     gene            2751..3356
                     /gene="recR"
                     /locus_tag="VFK00_06490"
     CDS             2751..3356
                     /gene="recR"
                     /locus_tag="VFK00_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7592635.1"
                     /translation="MSNESNLLAELVEALRCLPGVGAKSAQRMAFHLLERDRDGGSRL
                     ASVLEQAMQRIGHCRSCRNFSENDECAICASSSRDPSQVCVVETPADLLAIEQATGYR
                     GRYFVLMGRLSPLDGLGPQELGVDLLTRKLAEGEVREMVIATNPTVEGEATAHWLGQL
                     ARAARVRATRLAHGVPLGGELEYVDRGTLAHAFGTRQELQD"
     gene            3893..4237
                     /locus_tag="VFK00_06495"
     CDS             3893..4237
                     /locus_tag="VFK00_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET7592636.1"
                     /translation="MPDTIFDKIIRRELPADIVYEDDEILAFRDIHPQAPVHVLFVPK
                     QSFATLNDCSEADAALLGKLLLAASAYAKREGFADSGYRCVINCNKGGGQTVYHLHVH
                     LLGGRQMHEGMV"
     gene            4318..>4695
                     /locus_tag="VFK00_06500"
     CDS             4318..>4695
                     /locus_tag="VFK00_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592637.1"
                     /translation="MQRYVSRRGSAFVAGILIGLLAAASASAAGPAASQLMNDPDAPY
                     RVFVTKIGKTFVKDHAFVGRFATTVSMPDGTRRTIELVPTIHDGGFVVKLDDTVHGKH
                     VGSNGNSYMTPNGSTTNGTSATGT"
BASE COUNT          817 a         1520 c         1590 g          768 t
ORIGIN      
        1 gcgaggggcg agcatcatgg atgatgcgaa catatcccgc ccgtcggtgg gatgagaaca
       61 aaatctggag aggtgtccga gcggttgaag gagcacgcct ggaaagtgtg taagcggcta
      121 aaccccgctt cgcgggttcg aatcccgccc tctccgccag ttttcatggt ggccccgtcg
      181 gtgcctccgc gacgataaat cgaaaacccc gccagggccg gaaggcagca acggtatcga
      241 tcgactcgtg cgccggagat ccgctggcgg ggccgccgcc attttgtgct gctagacgaa
      301 ccgtccatgg tgcggagcgc ctgcccggac atccctgtcc ggtcgtgatt cgccgcgcga
      361 cctgccccca gcaggtcgca agcgcggcga atcacccccc gacgacgata aatcgaaaac
      421 cccgccaggg ccggaaggca gcaacggtat cgatcgactc gtgcgccgga gatccgctgg
      481 cggggccgcc gccattttcg cggcggccag tagcccggat gtagccgcac cgcggcggaa
      541 tccggatgat cctccaattt tcccggattt cgcttcgctt catccaggct actgtcttgg
      601 tcgcttcggc gcttggtctc gccgcgccga cttggcacaa tgggtggtcg cccacagatc
      661 gtgcccatga gctatcaggt acttgcgcgc aaatggcgcc cgaagcagtt cgaggaactg
      721 atcggacagg aacacgtcgt caaggccctg agccatgcgc tggattcggg ccgcgtccac
      781 cacgcgtacc tgttcacagg aacccgcggt gtcggcaaga ccacgatcgc gcggatcttc
      841 gcgaagtcgc tcaattgcga acgcggcatg tcggcgcatc cctgtggcga atgcgcgatc
      901 tgcaaggcag tggacgccgg ccgtttcgtg gaccttatcg agatcgacgc ggccagcaac
      961 accggcgtgg acgacgttcg cgacctgatc gaaagcgcgc agtacgcgcc ggcgcagggg
     1021 cgcttcaagg tgtacctgat cgacgaggtg cacatgctct ccaaatcggc cttcaacgcc
     1081 ttgctgaaga cgctggaaga gccgccggag cacgtcaagt tcctgctcgc gaccaccgat
     1141 ccgcagaagc tgctggtgac ggtgctttcg cgctgcctga agttcggctt gaagcgcctg
     1201 ttgccggagc agatcgcggg gcagatgcgg atgatcctgg ggcaggaagg cgtggctttc
     1261 gacgaaggcg cgatcgaggc gctcgcgcgc ggcgcggacg gttcgttgcg cgatggcctg
     1321 tcgctgctcg accaggcgat ctcccacggc ggcggggcgc tgcgcgcgga cgacgtccgg
     1381 gcgatgctgg gcacgatcgc ggacaacgac gtcggcctct tgttgcgcgc gctcgccgcc
     1441 ggtgacggca aggcgctgat ggaagaggcc gaccgaatcg ccggtctcgc gcccgatttc
     1501 gcgatggtgc tggagcaatt ggcaaacgcc ttgcatcgcg tgcagttgcg gcaattggtc
     1561 ccgggcttcc ggggcgacga agccgaacgc gaagtcctcg atgcgcttgc cggcgaggtc
     1621 gatcccgagg acgtgcaggt gtggtaccag tttgccgtcg gcggccgtcg tgacctgccg
     1681 ttcgcgcccg acgtgcggac cggtttcgag atggccttgt tgcggatgct ggcctttcgc
     1741 ggcggcgcgg cggatgccgg acagccggcg cggcgcgcgg caccggtgac gcgttcgcag
     1801 gcgcagcccg cgccgaccgg cgccacgcgc gctgcagcga cgcagccgag cgcttccgcc
     1861 ggcgtggccg acgcgccgca gcagcccgcg gccaaggcac ccgcgccgcg catcgacggg
     1921 ttggcggact gggaagcctg gatccagctg gccgaactgg gcggtccggc cgggcaactc
     1981 gcgcggcacg cgctgccgaa atccttccag ggcgacgtgc tgaccctggc actgacgccg
     2041 gaacacatga ttttctgcag tgatgccttg tgccatcagt tgcaggagaa gctgggcgag
     2101 gcgatcggcc gtcccctgcg cgtgcgggtc gtgtcggggg aagccgtcgc cgagaccccg
     2161 gcggcgcgcg ccgccaaggc ccgctccgaa caacaggccg cggccgagcg cgcgatggcc
     2221 gacgacccga tcgtccagga cttccagcgc cagctcggcg cggaaatcat ccccgaaagc
     2281 attcgacccg caggtacgcg agcatgaaag gacagttggg acagttgatg caacaggcgc
     2341 agcgcatgca ggaggaactg aagcgcgcgc aggaggaaat cgccaagctc gaggtcaccg
     2401 gcagcgccgg cggcggcatg gtcgaagtca cgatgaccgg ccgccacgaa gtgcgccgcg
     2461 tgcacatcga tcgcaagatg atggccgacg atccggagat gaccgaggac atgctggctg
     2521 ccgcggtcaa cgacgccgtc aacaaggtcg ccgaagccag ccagcagaag ctcggcggcg
     2581 tcagttcagg catgaacctg ccgccgggct tcaagttgcc gttttgaaca gggtcagaaa
     2641 ccggcaaaaa ccgggtcaga gtcacttttc caggggcgat ccacgggcgc gctgagtcgt
     2701 ttccggaaaa gtgactctga ccccggtttc tgaccccgct ttcgcaagcc atgtccaacg
     2761 aatccaacct cctcgccgaa cttgtcgaag cgctgcgctg cctgccgggc gtcggggcca
     2821 agagcgcgca acgcatggcg ttccatcttc tggaacgcga ccgtgacggg ggctcccgac
     2881 tcgcgtcggt cctcgagcag gccatgcagc gcatcggcca ttgccggtcg tgccgcaatt
     2941 tcagcgagaa cgacgagtgc gcgatctgcg cgagttcctc gcgcgatcct tcgcaggtct
     3001 gcgtcgtcga gacgcccgcc gacctgttgg cgatcgagca ggcgaccggt tatcgcggtc
     3061 gctacttcgt gttgatgggc cggctgtcgc ccctcgatgg cctcgggccg caggaactcg
     3121 gcgtggacct gctgacccgc aaactggccg aaggcgaggt tcgcgagatg gtgatcgcga
     3181 ccaatcccac cgtggaaggc gaagccaccg cgcactggct cggccaattg gcgcgcgcgg
     3241 cgcgcgtgcg cgccacccgg ctcgcgcacg gagttccgct cggcggcgag ctcgaatacg
     3301 tcgatcgcgg cacgctggcg cacgcgttcg gcacgcggca ggagctgcaa gactgagggg
     3361 cggacgtggc gcccaggcgt cccttgcctt ccacgcgggc acacaggtgg ggaggcgcct
     3421 ggaaagcggc ctggagctgc gccaaccgat gtcggttcca ctggcttgca cagccgtcca
     3481 tgcacggtcc cccggtggtg ggcgtgtcga accgatttac accttccgga tgcggcggaa
     3541 tatgcgccgc ggcaatggga atcacggccc ctgggtggtg ccggcctgtc ggcgggcatc
     3601 acaggtcagt gcttggccga catctgcttt tggccagaag ccctgcacga acatgatcca
     3661 ggtggcgtag cgccgggcca ccgtaacgtg gcccggccag gtggcgctgc atggcgtacg
     3721 cgactcgctt cgggcacggg ctttttcggc ggtgaactcg agtacgtcga ccgcggcagt
     3781 tggcgcgagc gttcggcagc cgcgggaatc ctgcggatcg acgcgtgggt cggggctcgc
     3841 gggtatgacg ttgttatgct gttccgagtc ccgaacgtga gctgcagccg ccatgcccga
     3901 caccatcttc gacaagatca tccgccgcga actgcccgcc gacatcgtct acgaggacga
     3961 tgaaatcctc gcgttccgcg acatccatcc gcaggcgccg gtgcacgtgc tgttcgtccc
     4021 gaagcaatcc ttcgcgacct tgaacgactg cagcgaggcc gatgctgcgt tgctcggcaa
     4081 gttgctgctg gcggcatccg catacgccaa gcgcgaagga ttcgccgaca gcggctatcg
     4141 ctgcgtcatc aattgcaaca agggcggcgg acagaccgtg taccacctgc acgtgcacct
     4201 gctgggcggt cggcaaatgc acgaaggcat ggtttgaggg ccgacgtcgc gggtgtttgg
     4261 tgggcggtgc acgtcccagt gggcgtgggt tttcccggaa ctcaatggag gtgtcccgtg
     4321 caacggtacg tttcccgccg cggttcagca ttcgtcgctg gaattctcat cggcctcctg
     4381 gcggcggcat ccgcctctgc ggccgggccc gcagccagcc aattgatgaa cgacccggac
     4441 gcgccctacc gggtcttcgt caccaaaatc ggcaagacat tcgtcaagga ccacgcgttc
     4501 gtcggccgtt ttgcgactac cgtgtcgatg ccggacggca cacgccgcac gatcgaattg
     4561 gtcccgacga tccatgatgg gggcttcgtc gtaaaactgg acgacacggt ccacggcaaa
     4621 cacgtggggt cgaacggcaa cagctacatg accccgaatg gatcgaccac caatggaaca
     4681 tccgcgacag gcacc
//
LOCUS       DASSNH010000174         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1389784, whole genome shotgun sequence.
ACCESSION   DASSNH010000174 DASSNH010000000
VERSION     DASSNH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389784"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VFK00_06505"
     CDS             <1..1604
                     /locus_tag="VFK00_06505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET7592638.1"
                     /translation="EDKPLASATDAKGTHLKAALHFDDASKAPLLVKVGISAVDLDGA
                     LRNLETELSDWDFERVQRTAAEQWERELSRVRIASESEDVLKTFYSGLYHTMLAPTLF
                     SDVDGRYRGMDLEVHTLPKGRHNYSTYSLWDTYRALHPLYTLFQPDKVPDLVQGLVRK
                     ANESPQGPAVWTLQGVETGTMIGYHSVVVIAEAQAKGFTGIDYAQAWPVYRKRAMSDD
                     YRGLPWYRKLGYIPSDKEGEAVSKTLEYAYDDWAMAHFAESVGEHADAKALRERSRNY
                     HNVFDRKLSFMRPRGADGRWLEPFSPIEMGHSNKWRDYTESNPWVATFLNQHDLYHYM
                     TMFGGESAFERKLDELFTTASTLPPNAPPDIAGMVGQYAHGNEPSHHIAYLYAYSGSH
                     WKTQARVRMLLETMYRPAPDGLAGNEDCGQMSAWYLLGALGLYAVDPVSANWVFGSPL
                     VDHAEIDVGEGRKLVVDARNNGEGRPYIQSVTWNGKPWTKSWIRHAELAKGGRLVFEM
                     GGQPNKQFGAAPADRPPSFGQPASA"
     gene            1719..>2257
                     /locus_tag="VFK00_06510"
     CDS             1719..>2257
                     /locus_tag="VFK00_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HET7592639.1"
                     /translation="MPMVRLSSIASVAVATLFALVLALPAVAASDALTQVDPMIGTGG
                     NGHTFPGATVPFGMIQLSPDTAMPDFKHAYPWAAGYQYGDHSIMGFSETHFSGSGHSD
                     LGDVLLMPGVGKVQWDPGDPDKPGSGYRSDFSHETEVAQPGYYAVTLATSGVRVELTA
                     GKRVGWHRYTFPKGQDAHVL"
BASE COUNT          425 a          719 c          752 g          361 t
ORIGIN      
        1 cggaagacaa accgctcgct tccgcgaccg acgccaaagg cacccacctg aaggccgcgc
       61 tgcacttcga tgacgcctcg aaggcgccgc tgctggtcaa ggtcggcatt tcggcggtgg
      121 acctcgatgg cgcgctgcgc aatctggaaa cagaactttc cgattgggac ttcgaacgcg
      181 tgcaacgcac cgccgcggag caatgggaac gcgaactctc gcgagtgaga atcgcatcgg
      241 aatccgaaga cgtgctgaag acgttctaca gcggccttta ccacacgatg ctggcgccga
      301 ccctgttcag cgacgtcgac gggcgctacc gcggcatgga cctcgaagtg cacaccctgc
      361 ccaagggccg gcacaactac agcacctatt cgctgtggga cacctaccgt gcgctgcatc
      421 cgctgtacac gctgttccag ccggacaagg tgccggatct cgtgcagggc ctggtgcgca
      481 aagccaacga gagcccgcag ggtcctgcgg tatggacgct gcagggcgtc gagaccggga
      541 cgatgatcgg ctatcactcg gtggtggtga ttgccgaggc gcaggccaag ggcttcaccg
      601 gcatcgatta cgcgcaggcg tggccggtgt atcgcaagcg cgcgatgagc gacgattacc
      661 gcggactgcc gtggtaccgc aagctcggct acatccccag cgacaaggaa ggcgaggcgg
      721 tcagcaagac cctggaatat gcctacgacg actgggccat ggcgcatttc gcggaaagcg
      781 tcggcgagca cgccgatgcg aaagcccttc gcgagcgttc gcgcaactac cacaacgtgt
      841 tcgaccgcaa gctcagcttc atgcgcccgc gtggtgcaga tggccggtgg ctcgagccgt
      901 tcagtccgat cgagatggga cactcgaaca agtggcgcga ctacaccgag tcgaacccct
      961 gggtcgcgac cttcctcaac cagcacgacc tgtaccacta catgacgatg ttcggaggcg
     1021 agtctgcctt cgaacggaaa ctcgacgagc tcttcaccac cgcgtccacg cttccgccga
     1081 acgcgccgcc cgacatcgcc ggcatggtcg gccagtacgc gcacggcaac gaaccgagcc
     1141 atcacatcgc atacctgtac gcgtattccg gctcgcactg gaagacccag gcgcgcgtgc
     1201 ggatgctgct ggaaaccatg taccgacccg cgccggatgg cctcgccggc aacgaggact
     1261 gcgggcagat gagcgcgtgg tacctgctgg gcgcgctcgg cctgtatgcg gtcgatccgg
     1321 tcagcgcgaa ttgggtgttc gggagcccgt tggtggacca cgccgaaatc gacgtgggcg
     1381 aaggccgcaa actggtggtg gatgcgcgga acaacggcga agggcggccg tacatccagt
     1441 cggtgacctg gaacggcaag ccgtggacga agagctggat caggcacgcc gaactcgcga
     1501 agggcggcag gctggtgttc gagatgggcg ggcagccgaa caagcaattc ggtgcggcgc
     1561 cggccgatcg tccgccgtcg ttcggccagc cggcatccgc gtgaccactc gtcgtgcgcc
     1621 acgcgtggcg cgagcatcgg cgcgtgccga ccggcacggg tagccgggat ccgcgcgcgc
     1681 tacgatcgcg cgagtcgttt cgcctcgaga ggtgcgtcat gcccatggtc cgtctgtcgt
     1741 cgatcgcatc tgtcgcagtt gcgacgctgt tcgcgctggt gctcgcactt ccggccgtcg
     1801 cggccagtga tgcattgacg caggtcgacc cgatgatcgg caccggcggg aacgggcaca
     1861 catttccggg tgccacggtg ccgttcggaa tgatccagct aagccccgac accgcgatgc
     1921 ccgatttcaa gcacgcctat ccgtgggcgg cgggctacca atacggcgac cacagcatca
     1981 tgggtttttc ggagacgcat ttttccggga gcgggcattc cgatctgggc gacgtgctgc
     2041 tgatgccggg ggtgggaaag gtgcaatggg atccgggtga tcccgacaaa ccggggtcgg
     2101 gctaccgttc ggatttcagc cacgagaccg aagtcgccca gccgggctac tacgcagtga
     2161 cgctggcgac ctccggcgtg cgcgtggaac tgaccgcggg caagcgcgtc ggctggcatc
     2221 gctatacgtt cccgaagggc caggacgcgc acgtgct
//
LOCUS       DASSNH010000175        17307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1067012, whole genome shotgun sequence.
ACCESSION   DASSNH010000175 DASSNH010000000
VERSION     DASSNH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 17307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17307
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067012"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="VFK00_06515"
     CDS             complement(<1..79)
                     /locus_tag="VFK00_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592640.1"
                     /translation="MSKEKFERKKPHVNVGTIGHVDHGKT"
     gene            complement(218..293)
                     /locus_tag="VFK00_06520"
     tRNA            complement(218..293)
                     /locus_tag="VFK00_06520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Thr,seq:ggt)
     gene            complement(421..494)
                     /locus_tag="VFK00_06525"
     tRNA            complement(421..494)
                     /locus_tag="VFK00_06525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Gly,seq:tcc)
     gene            complement(540..625)
                     /locus_tag="VFK00_06530"
     tRNA            complement(540..625)
                     /locus_tag="VFK00_06530"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Tyr,seq:gta)
     gene            complement(965..1414)
                     /locus_tag="VFK00_06535"
     CDS             complement(965..1414)
                     /locus_tag="VFK00_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET7592641.1"
                     /translation="MAVLVVAGCGTRAAREPAFAEPPTASEPVAGTTMAKLDGTQWRF
                     VEVAGAPVPAAVTATLRIRDGRISGRAGCNSFGAKYLADADGKGSFSQIMSTKMACVE
                     PAGAMDVGHRIFEALPKVSRMDLADGELALLDAAGKPLARLEPAAGS"
     gene            complement(1480..2619)
                     /locus_tag="VFK00_06540"
     CDS             complement(1480..2619)
                     /locus_tag="VFK00_06540"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HET7592642.1"
                     /translation="MQSIECRPLGRLCARLLALAMLMGLGGMGPARAVESGYQYFSVG
                     DPAAPTTAKTSPGLLLMGGGREIEAAFQWFAAQAGHGHIVIIRASGDDQLQDWIYRDV
                     GGVASVQTLIFHSRAPASDPRVLGILRHADGIFIAGGDQANYVRFWKGTPLNDVLNAH
                     VRAGKPIGGTSAGLAILGQWGYGALDGGSITSPEALGNPAGPAVTLVDHFITLPLLEG
                     TITDTHFMQRERLGRLLAFVARIAKDHHTAAVTGLGVDQDSALLVEPDGSARLLSWHP
                     DGHAWVVVPGKLRAVERAGGQAALDDYLRDRVVPGKPLDLPGYVVTGIGTGSGLRLPG
                     YEVTDPAFRRHYDIVGGRLVERPSAAEEQRASRAARIDQHEAGPG"
     gene            2764..3333
                     /locus_tag="VFK00_06545"
     CDS             2764..3333
                     /locus_tag="VFK00_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7592643.1"
                     /translation="MNEVLDAWFAREVLAHEGALMRFLHRNWRDHDEVVDLRQEIYVR
                     VYEAAGRRRPDSPKAFLFATARHLLADRIRRQRIVSITAVGDLEQLNVLTDELEPGRW
                     LGGRDALERLARALDRLPDRCREVVWLRRVEDMPQKEIARRLGISEKTVEKHIAKGCR
                     MMANHFYAASRGDGTRTGDGSVTARKDSP"
     gene            3333..4406
                     /locus_tag="VFK00_06550"
     CDS             3333..4406
                     /locus_tag="VFK00_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET7592644.1"
                     /translation="MGRSGGQVERIAASWLARRDGDHWSLADQSALAAWLEQDPAHRV
                     AFLRLQAAWRETGRLKALAAGTPQGQLPERGAWRRSPYFNPPSFQRGDASGRGRRHGS
                     RPSRFASPVAAIAAGLLVILLAGGFWWWRDAGRVERRVWQTELGGQKVVHLTDGSTAT
                     LAGDSELHASMTRRERDLDLVRGEAFFEVAKDHARPFVVHASGYRVIAVGTRFDVRRE
                     SDGLRVVVTRGVVRLQSAVDPGRAPTELPAGSIARVRNDEVFVQHVGPEQALQHLGWR
                     SGYAVFHDTPLVEAIGEFNRYNARQIVIADPSLDDLKVGGNFRLDNSDAFVRLLQQVF
                     PVHAERRGDRIMLSRAGRKQAPD"
     gene            4493..7609
                     /locus_tag="VFK00_06555"
     CDS             4493..7609
                     /locus_tag="VFK00_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592645.1"
                     /translation="MLKPIVRFALLFACLLPALAFAQNPDTREIDISAGSLVAALDSL
                     GHQTGMQFVYDADRLADARTRGVHGTLTGQQALAGLLAGTGYHARRDASGAMVIVQDQ
                     PTAAPAAPPPTSSPAAGQGAGEQGPTTTLQEVVVTGSRIPRSEIEGPAPVVTITAQDI
                     QRRGFASVPELMTSLTQNLGALDNNQYTDGFSPGAQAVDLRGLGPNHTLVLVNGRRIA
                     DYPQSYNGNSNFTDISNIPTALIDRVEILSGSASAVYGSDAISGVINFILKEKADGTT
                     LNYRAGGTQHGGGGSQRLDITSGFSKGKFDAVFGLELIDKHPLWAFQRSFTDSRLDSP
                     ADPSDIIASRNFIRTDEDGDYLDPGKATCDKLSYLDNGTMFYASRDEYGYDPVTDSYV
                     PGYYCGTYADVGYGTLENGSRMANLFASATYHFNDYTDFYADVLAGTSHQETYNTPLQ
                     WYNCEPLNGDCTDTPFYNTATGQIEEWGRSYFTIDENGGFERGKIRNIGNTLSLNTGI
                     KGTFGRDSDWNYEANFRHSQNEVESKWPALIAAKAQALYLGPSLGDYIDEDGNDTGYQ
                     MYYAPVSRLYTPLTVEQFRSISQDSIDNDKTRAENYSLTLTNTNLFKLPAGPVGFAGI
                     AEYGNQYMAQIADPLSLDGSYYGLHNTTSVGSRSHAAIGYEFSIPVFSQLTVTTAGRY
                     DRYKYSETSTGKFTYALGLEYRPFDSLLLRGSLSTGFRAPDLSYLYAGLSGSSSGGTD
                     YYQCRRDNGPGYDCIGDSTYDADFNGRSHGSTTLKDETSKSFTYGFVFAPTPNFDVSA
                     DYYRIKLNNEVEYQDSDTILRREADCRLGQDVSGNPIDGNSAFCQQIDSQVVRNPADA
                     GYNPEGITSVLVLPINAAIDQTSGVDFNTHYRLRTARAGTFDFNLGATYVIKHTTQLS
                     PELPVDNEMTDIYYYLIPRTKANASVTWNYHDFTTTLYGARLGGIPNYDGTYRLGPTF
                     LYNLTMGYRVTPGIDVSLVVDNLFDKRPPRDPSWTSYPYYSRNWFNPIGRAYFVQMSL
                     KFGGTNGI"
     gene            complement(7736..8827)
                     /gene="ychF"
                     /locus_tag="VFK00_06560"
     CDS             complement(7736..8827)
                     /gene="ychF"
                     /locus_tag="VFK00_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7592646.1"
                     /translation="MAIQCGIVGLPNVGKSTLFNALTKAGIAAANFPFCTIDPNVGVV
                     PVPDARLQQLAEIVHPQKVIPASVEFVDIAGLVAGASKGEGLGNQFLAHIREVDAIAH
                     VVRCFEHPDVVHVTGKVDPIADIETIDTELALADLESVDKALARVERAAKANDKEAMA
                     RRPVLEKLAAALNQGKAARSVALDDEERAAMRDLFLLTIKPLMYVANVREDGFEDNPH
                     LDAVRRRAETEGAVVVPVCAAIEEELAQLDEADRGEFLASMGLSEPGLDRVVRAAYTL
                     LGLQTYFTAGPKEVRAWTVKQGATAPQAAGVIHTDFEKGFIRAETVSFDDYMKYRGES
                     GARDAGRLRLEGKEYRVQEGDVLHFRFNV"
     gene            complement(8900..9505)
                     /gene="pth"
                     /locus_tag="VFK00_06565"
     CDS             complement(8900..9505)
                     /gene="pth"
                     /locus_tag="VFK00_06565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7592647.1"
                     /translation="MGIRLIAGLGNPGAEYLRNRHNAGFWFADALASGAGVRFASEGR
                     LHAAACRVPIGVEQVRVIKPATFMNESGRAIVAALNYWKLPPAELLVVHDDLDLPPGT
                     VRLKFDGGHGGQNGLRDIMTQLGHGRFHRLRIGIGHPGDRNRVTPWVLGNPSRADEDA
                     MLDAIARALNVLPLTIAGEFNEAMKRLHTQTAGTGNGERGT"
     gene            complement(9596..10303)
                     /locus_tag="VFK00_06570"
     CDS             complement(9596..10303)
                     /locus_tag="VFK00_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET7592648.1"
                     /translation="MATIHEITAERRADQGKGASRRLRRAGKVPGVVYGAGTDAENVQ
                     FEHETVVLASRHDWFASSILDLLVEGKRQKVLLRDFQKHPVKEQVLHLDFLRIDEAKP
                     IRVYVSIRFINKEKSAAGKTSGVVISHNLTEVEVSCLPKDLPEFIEVDLADLELGDSR
                     HLSDLKLPEGVEIPELKLGKEYDHTVVSAQAVREEVEEAPAEAGAEGAPAEGAAAAAA
                     PSGEAVKAAAPAKEEKK"
     gene            complement(10451..11410)
                     /locus_tag="VFK00_06575"
     CDS             complement(10451..11410)
                     /locus_tag="VFK00_06575"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET7592649.1"
                     /translation="MHNASSLLLFSGNANRKLAEDVARTLGEPLGKAVVGQFSDGEVQ
                     IEIDENVRNQDVFVMQSTCAPVAENLMELLVLVDALKRASAASVTAVIPYFGYARQDR
                     RLRAARVPITAKVAAQLIGAVGTDTVLTVDIHADQIQGFFDIPLDNVYASPVLLADIW
                     RKHGTEGVTVVSPDVGGVVRARAVAKRLDDADLAIIDKRRPRPNEATVMNIIGDVVGR
                     TCVLVDDIVDTAGTLCAAAKALKDRGAKEVVAYCTHAVLSGPAVRNIEQSLMDELVVT
                     DTIPLSEEGAACGKIRQLSVAQLLAETVRRMAYGESVSSLYVD"
     gene            complement(11444..11515)
                     /locus_tag="VFK00_06580"
     tRNA            complement(11444..11515)
                     /locus_tag="VFK00_06580"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11481..11483),aa:Gln,seq:ttg)
     gene            complement(11717..12385)
                     /gene="lolB"
                     /locus_tag="VFK00_06585"
     CDS             complement(11717..12385)
                     /gene="lolB"
                     /locus_tag="VFK00_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HET7592650.1"
                     /translation="MTRWLRLAVAAGLAGLVLAACAPVPVRHPGTAAQLAAQTARERM
                     LGAQAQWGLHARFAASDGDHGGSGSLDWSQQGDRYRFTLRAPITGKTVELDGGPGGAV
                     LTGAGREPLAGRNAGEVLNAEFGWNVPVADLAWWVRGLRAPGAPAILTFGANGLPATL
                     DQDGWHVDYRDWYAERSPPLPRKVYASRDPYSVRLSIQEWNGASAESAGGAAAAHGRS
                     DPER"
     gene            complement(12382..14205)
                     /locus_tag="VFK00_06590"
     CDS             complement(12382..14205)
                     /locus_tag="VFK00_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592651.1"
                     /translation="MGIVRTQPDKFKKLDPGWAPIRVLARMKPSWFHFSAPAVVLAVA
                     WLAGCAPLSPRAGSRQPTGYGPVAEAQPLARLDIEPSGPAAKASALLIAAEFALQDQD
                     IDEAARDYAAAAEIADDPAVAKRALQLAVASGETANAKKLLDRWRALGAAPRDLAGIR
                     AQLAMLRGDRSGAEKDFDILLATGGADAWKTFAADLLAARDTALAGRILEDLAVAERL
                     PRNENVWVALSQLGEHLGRHAFARKLADAAVQRFGGTDSIRWAASLKLSTGDSAGARA
                     LYAKGVAAHPRDPALRLGYASVLGAAHEYGEALRVLADGPQTPRTWSARVAFAARAKD
                     DAALQRLYAELKRSPPGQRADNAFLLGQLAELMHHDSQALDWYGQVDVDSEHGFEAKV
                     RSAVLLDKTGKQAQAHALAAQLQQDYADDPDSLRTAYELDAQLYAQHGDHARAIAVYD
                     RGLGALPGDPALIYDRGIEEANAGNTDAALRDFREVLARNPENIEAMNALGFTLADAD
                     RDLPEATRLLRKALAAKPDAAAIMDSWGWLQYRLGHLDEAESYLQRAWASHEDPDIGA
                     HLGEVLWKLGQHRDAREVFAKVRKLDPHNAALQHAERRLRP"
     gene            14274..15536
                     /gene="hemA"
                     /locus_tag="VFK00_06595"
     CDS             14274..15536
                     /gene="hemA"
                     /locus_tag="VFK00_06595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET7592652.1"
                     /translation="MPLIALGLNHLTAPVSLREQVAIDAEATAPALADLASQAGVDEA
                     LILSTCNRTELYCSVRDGAEGAPSAWLHTHNQMTPGRLDEFLYRHTDGDAVRHVFRVA
                     TGLESMVLGEPQILGQVKDAWRAARSARTLQAPMDRLLQNAFTVAKRVRTETRIGSGP
                     VSVAFAAVRLVEQAFADLRESCVLLIGAGDTIELAIRHLIERGVRRLIVANRTIETAQ
                     ALINPVGGYAIALADLPKHLAEADIVMSATASRVPILDAPMLRGALRQRRRRPMLLMD
                     LAVPRDIEPSVAELPDLFLYTIDDLQQAVDVGRRSREASVREAEAIIDLQVERYLAWR
                     RANQFDQPLRCYRATAEAQRDEVLARAREMLAAGRAPDQALEYLANTLTGRLLHTPSV
                     KLHEAAEHGDQALLDAAARLFDMNGRDE"
     gene            15533..16615
                     /gene="prfA"
                     /locus_tag="VFK00_06600"
     CDS             15533..16615
                     /gene="prfA"
                     /locus_tag="VFK00_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7592653.1"
                     /translation="MNPQLKRRLEQLLERHHEVGLLLAEPGVANDPAHFRELSREYAQ
                     TQELAEVFAESQKGEAELAHARAMLADPDLRELAREEVARLEQSSAHLDSRLQALLTP
                     RDPLDDANLFLEVRAGTGGDEAALFAGDLLRMYLRYAERRGWRAELMSAHEGEHGGYK
                     EAVARIEGSGAYAALKFESGTHRVQRVPVTEASGRIHTSAATVAILPEQAEIADVELR
                     DADLKVDTFRASGAGGQHVNKTDSAIRITHLPTGIVVECQDERSQHKNRARALSLLKA
                     RLLDAQRSEQTAQQAATRKLQVGSGDRSQRIRTYNFPQGRVTDHRINLTLHQLPQILD
                     GDLDELVGALARESHAEALAALGEAP"
     gene            16612..>17307
                     /locus_tag="VFK00_06605"
     CDS             16612..>17307
                     /locus_tag="VFK00_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592654.1"
                     /translation="MNDARNADLRTRLANIAGLLERREAAGAERLAADARAAHPQASE
                     AARLHGIALLMLDRGTEARDALETALRLQPDSPEAGSNLASALLALGDAAGAVALLER
                     VVGARPNDPALRNNLANALRAAGDDSRAREEYLAALAAAPGHFGASANLAAVEISLGM
                     LDEAEARLRSLLAAQPHPQLYLLLGHVLHRRHRFADAQAAYLQGAQLAPGAAELAYQA
                     ALMADEQHHYAEAA"
BASE COUNT         2871 a         6067 c         5571 g         2798 t
ORIGIN      
        1 tcgtcttgcc gtggtccacg tgaccaatcg tgcccacgtt cacgtgcggc ttcttgcgct
       61 cgaatttctc tttggacatg gcgtttgtac cttgtgactg actcgtagat tgattgcttc
      121 gtgcttcttc aaaagcggga acagggaacg ggagtgtgtg ccgcttcgct tttgttccct
      181 gttcccgctc ttcgtttccc gcttcttcag ctctcagtgg tgctcacgac aggaatcgaa
      241 cctgtgacct cttccttacc aaggaagtgc tctaccgact gagctacgtg agcatcgata
      301 tccattgcga tgatttttcg ggcatcctgc cgatgtgcgc gacagagccg cgccacgtgg
      361 tcaactcgcg cttcgcgccg gcacttcacg tgtcggttgc agttcctcgg gcagacccaa
      421 tggagcggga gacgggaatc gaacccgcac catcagcttg gaaggctgag gttctaccat
      481 tgaactactc ccgcgtcggc ggcgttctct ggcggagcct tggctccgtt gcatgaaact
      541 ggtggaggga ggtggattcg aaccaccgaa ggcgtaagcc agcagattta cagtctgccc
      601 ccgttggccg cttgggtatc cctccgaatt ctcttttcct gtatcactat ctgggaggaa
      661 cgctgcgaac caccgaaggc gcaagccagc agatcacact tcctgtctgc cccgctgggt
      721 agccctccga attcccttgt cttgtaccta tggcggccca gcgacacgaa caacccggca
      781 tgcgcgacga cacccgggtt cgagcagttt gcatcacaaa gaccgcgtat ttttgcggct
      841 cccaggctgc aagtcaacct ccggcgaagg ttcgggagca tcctgaacgg ccattggcgc
      901 cgtggccgcg cgacacacgt gccgcgaaag ggagtccggc ggcccgagcc gccgcgctgc
      961 tggatcagga gccggcggcc ggctccagcc gggccagcgg cttgcccgcg gcatcgagaa
     1021 gcgccagttc gccatccgcc aggtccatcc gggagacctt cggcaaggcc tcgaaaatcc
     1081 gatgcccgac atccatcgca cccgccggct cgacgcacgc catcttcgtc gacatgatct
     1141 ggctgaacga acccttgcca tcggcgtcgg ccagatactt cgcgccgaaa ctgttgcagc
     1201 ccgcccggcc ggagatgcgg ccatcgcgga tccgcagggt cgccgtcacg gccgccggca
     1261 ccggggcgcc ggcgacttcg acgaaccgcc attgggtgcc atcgagttta gccatcgtcg
     1321 tgccagccac gggctccgat gcggtcggcg gctcggcaaa cgctggttcg cgggccgccc
     1381 gcgtaccgca accggccacc accagcaccg ccatcaacac ggccatccgc atgatcgagc
     1441 cctctgcatc aggtcccctc catgataggc ggttcggcct taacctggtc ccgcttcgtg
     1501 ctggtcgatc cgggcagccc gagaggcgcg ttgttcctcg gcggctgacg gtctctccac
     1561 caggcggccg ccgacgatgt cgtagtgcct gcggaacgca gggtccgtca cctcgtaccc
     1621 cggcaaccgc aaaccgctcc cggtaccgat gccggtcacg acgtaaccgg gcaggtcgag
     1681 cggcttgccg ggaacgaccc gatcccgcag gtaatcatcc agtgccgcct gcccaccggc
     1741 gcgttcgacc gcgcgcagct ttcccggcac caccacccag gcgtggccgt cggggtgcca
     1801 gctcaacaac cttgcggatc catccggttc caccagcagc gcactgtcct ggtccacccc
     1861 gagcccggtg acggcagcgg tatggtggtc cttcgcgatc ctcgccacga acgccagcag
     1921 gcggccgagc cgctcgcgct gcatgaagtg cgtatcggtg atggtgccct cgagcaacgg
     1981 cagcgtgatg aaatgatcga ccagggtcac ggcgggaccc gcggggttac ccaaggcctc
     2041 gggcgaggtg atgctgccgc cgtcgagcgc gccgtaaccc cactgcccca ggatcgcgag
     2101 ccccgcgctg gtgccgccga tcggcttgcc ggcgcgaacg tgcgcgttca acacgtcatt
     2161 cagcggcgtg cctttccaga agcgcacgta gttggcctgg tcgcctccgg cgatgaaaat
     2221 gccatcggca tgccgcagga tcccgagcac ccgcggatcg cttgccggcg cgcgcgaatg
     2281 gaaaatcagg gtctggaccg acgccacgcc gccgacgtcg cggtagatcc agtcctgcaa
     2341 ctggtcatcg cccgacgcgc ggatgatcac gatgtgccca tggccggcct gcgcggcgaa
     2401 ccattggaag gcagcctcga tctcgcggcc gccccccatc aacaacagcc cgggcgaagt
     2461 cttcgccgtc gtcggggccg ccgggtcgcc gaccgagaaa tactggtagc cggattccac
     2521 ggcgcgagcc ggacccatgc caccgagccc catcaacatc gccagggcga gtagccttgc
     2581 gcagagccgg ccaagcggtc ggcattcgat cgattgcatg ctgaaggctc ctccacggtc
     2641 gggcgccgcc gcgcggggcg ggcgaacgtg gggtcaggac gaaaggcgcg gggcgttccc
     2701 ccatgccgtc agtcatgcaa cccgatggcc catggcggcg atccgatgcg gtaacttcac
     2761 gccatgaacg aggtcctcga tgcgtggttc gcgcgcgaag tgcttgcgca cgagggcgca
     2821 ctgatgcgct tcctgcaccg caactggcgc gaccacgacg aggtggtgga cctgcggcag
     2881 gaaatctacg tgcgcgtcta cgaggcggca ggccggcgcc ggcccgactc gcccaaggcc
     2941 ttccttttcg cgacggcgcg gcacctgctc gccgaccgga tccgtcgcca gcgcatcgtt
     3001 tccattaccg cagtgggtga tctcgagcaa ttgaacgtct tgacggatga actcgagccg
     3061 ggccgttggc tcggcgggcg cgacgccctg gagcgcctcg cgcgtgcact ggaccgcctg
     3121 ccggaccgct gccgcgaggt ggtctggctg cggagggtcg aggacatgcc gcagaaggaa
     3181 atcgcccgcc ggctcggcat cagcgaaaaa accgtcgaga agcacattgc gaagggatgc
     3241 cggatgatgg ccaatcattt ctacgcggct tcgcgcggtg acggcacccg gaccggcgac
     3301 ggcagcgtga cggcgcgcaa ggacagtccg tgatggggcg gagcggcggg caggtcgagc
     3361 ggatcgcggc atcgtggctg gcgcgccggg atggcgacca ctggtcgctg gccgaccagt
     3421 ccgcactcgc ggcatggctg gaacaggatc ccgcccaccg cgtggcgttc ctgcggctgc
     3481 aggctgcctg gcgcgaaacc ggaagactga aggcattggc ggcggggacg ccgcaagggc
     3541 agttgcccga acgtggcgcg tggcgccgct cgccgtactt caatccgccc agcttccagc
     3601 ggggcgacgc ctcgggccgc gggcgccggc atggttcgcg accatcccgg ttcgcctccc
     3661 cggttgccgc gatcgcggca gggctgctcg tcatcctgtt ggccggcggc ttctggtggt
     3721 ggcgggatgc cggccgcgtc gagcgccgcg tctggcaaac agagctgggc gggcagaagg
     3781 tggtgcacct caccgatggg tcgaccgcca ccctcgccgg cgacagcgag ctgcacgcgt
     3841 cgatgacccg gcgcgagcgc gatctcgatc tcgtccgcgg cgaggcgttt ttcgaagtcg
     3901 ccaaggacca tgcccgcccc ttcgtggtgc acgccagcgg ttaccgggtc atcgcggtcg
     3961 gcacccgctt cgacgtgcgc cgcgaaagcg atgggctgcg ggtggtcgtg acccgcggcg
     4021 tggtgcgcct gcagtcggcg gtcgatcccg gccgggcgcc taccgaactt cccgccggca
     4081 gcatcgcccg cgtcaggaac gacgaggtat tcgtccagca cgtgggcccg gagcaggcgt
     4141 tgcagcacct cggctggcgc agcggctacg cggttttcca tgacacgcca ctggtggagg
     4201 cgatcgggga attcaaccgg tacaacgcgc gccagatcgt gatcgccgat ccctctctcg
     4261 acgacctgaa ggtgggcggc aatttccgcc tcgacaacag cgacgccttc gtgcgcctgt
     4321 tgcaacaggt cttccccgtc cacgccgagc gacggggcga ccggatcatg ctgtcgcgag
     4381 ccggacgaaa gcaggcgccg gattgaaaac aattgccgcg cgagcggggg aatggccgcc
     4441 ctggctcgtc ttgtcacgca gggcgtcgcg tcgacgccgt tggggaggga acatgttgaa
     4501 gccgatcgtc cgctttgcat tgttgttcgc gtgcctgctt ccggcgctgg cattcgccca
     4561 gaacccggac acacgggaaa tcgatatctc cgcaggcagc ctggtcgcgg cgctcgactc
     4621 gctcggccat caaaccggca tgcagttcgt gtacgacgcc gaccggctgg cggacgcgcg
     4681 cacccgcggg gtccacggca cgctgaccgg ccagcaggcg ctggccgggc tgctggccgg
     4741 taccggctac cacgcccgcc gtgacgcctc gggcgccatg gtgatcgtgc aggaccagcc
     4801 caccgccgcg ccggcggctc ctccgccgac ctcatcgccc gcggcgggcc agggggccgg
     4861 cgagcagggg ccgaccacga ccctccagga agtggtcgtg accggctcgc gcattccgcg
     4921 gtccgagatc gaggggccgg ccccggtggt gacgatcacc gcgcaggaca tccagcggcg
     4981 gggcttcgcc agtgttcccg aactcatgac ctcgctgacc cagaacctcg gcgcgctgga
     5041 caacaaccag tacacggacg gcttctcgcc cggcgcacag gcggtcgact tgcgcggtct
     5101 cggtcccaac cacacgctgg tgctggtcaa tggccggcgc atcgccgact atccgcagtc
     5161 ctataacggc aatagcaact tcaccgacat ttccaacatc cccacggccc tgatcgatcg
     5221 cgtggaaatt ctttcgggca gcgcgtcggc cgtatatggg tccgacgcga tttccggtgt
     5281 catcaatttc atccttaagg agaaagccga cggcacgacg ctcaactacc gcgccggcgg
     5341 cacccagcac ggcggtggcg gttcgcagag gctcgacatc acctcgggct tctccaaggg
     5401 caagttcgac gcggtgttcg ggctcgaact gatcgacaag cacccgctgt gggcgttcca
     5461 gcgcagcttc accgattccc gcctcgacag ccccgccgac ccctcggaca tcattgccag
     5521 tcgcaacttc atacgcaccg acgaggacgg cgactatctg gacccgggca aggccacctg
     5581 cgacaaattg tcgtacctcg acaacggcac gatgttctac gcctcgcgag acgagtacgg
     5641 ctacgacccg gtgaccgatt cgtacgtgcc gggctactac tgcggcacct atgccgacgt
     5701 cggctacggc acgctcgaaa acggctccag gatggccaac ctgttcgcca gcgccaccta
     5761 tcacttcaac gactacaccg atttctacgc ggacgtgctg gccggcactt cgcatcagga
     5821 gacgtacaac accccgctgc agtggtacaa ctgcgagccg ctgaatggcg actgcaccga
     5881 cacaccgttc tacaacaccg ccaccgggca gatcgaggaa tggggccgca gctacttcac
     5941 catcgatgaa aacggcggct tcgaacgcgg caagatccgc aacatcggca atacgctgtc
     6001 gctcaacacg ggcatcaagg gcaccttcgg ccgcgacagc gactggaact acgaggcgaa
     6061 tttcaggcac tcgcagaacg aggtggagtc gaagtggccg gcgttgatcg ccgccaaggc
     6121 gcaagcactg tatctcgggc cttcgcttgg cgactacatc gacgaagacg gcaacgacac
     6181 gggctaccag atgtactacg cgccggtcag ccgcctttac acgcctctga ccgtcgagca
     6241 attccgctcg atcagccagg actcgatcga caacgacaag acccgcgccg aaaactattc
     6301 gctgacactc accaatacca acctgttcaa gctgcccgcc ggtccagtcg ggttcgccgg
     6361 catcgccgag tacggcaacc agtacatggc gcaaattgcc gatccgttgt ccctggatgg
     6421 gagctactac ggcctgcaca acacgacctc ggtcggttcg cgcagccatg cggcgatcgg
     6481 ctacgaattc agcatcccgg tgttttcgca actcaccgtg accaccgccg gccgctacga
     6541 ccgctacaag tacagcgaaa ccagcaccgg caaattcacc tacgcgctcg ggctcgaata
     6601 ccggccgttc gatagcctgc tgctgcgcgg ttcgctgtcc accggctttc gcgcacccga
     6661 cctgtcatac ctgtacgcgg ggctcagcgg atcgagttcc ggcggcaccg actactacca
     6721 gtgccggcgc gacaacgggc ccggctacga ctgcatcggc gactccacct acgacgccga
     6781 cttcaatggc cgcagccacg gcagcaccac gctgaaggac gagaccagca aatccttcac
     6841 ctacggcttc gtgttcgccc cgacgcccaa cttcgacgtc agcgccgact actaccgcat
     6901 caagctcaac aacgaggtcg aataccagga cagcgatacg atcctgcgcc gcgaagcgga
     6961 ttgccgcctt ggccaggatg tcagcggcaa tcccatcgat ggcaattcgg cattctgcca
     7021 gcagatcgac agtcaggtcg tgcgcaatcc cgcggatgcg gggtacaacc cggagggcat
     7081 cacttcggtg ctggtgttgc cgatcaatgc cgcgatcgac cagacgtcgg gtgtcgactt
     7141 caacacgcac taccggctcc ggaccgcgcg cgcgggaacg ttcgatttca accttggtgc
     7201 gacctacgtg atcaagcaca ccacgcagtt gagccccgag ttgccggtcg acaacgaaat
     7261 gaccgacatc tactactacc tgattccccg caccaaggcc aacgcctcgg tgacgtggaa
     7321 ttatcacgac ttcaccacga ccctgtacgg cgcgcgcctc ggcggcatcc cgaactacga
     7381 cgggacttat cgtcttggcc cgaccttcct gtacaacctg accatgggct accgggttac
     7441 gcccggcatc gacgtgtcgc tggtcgtcga caacctgttc gacaaacggc cgccacgcga
     7501 cccgtcatgg accagctatc cgtattactc ccggaactgg ttcaatccga ttggacgcgc
     7561 ctatttcgtg cagatgagcc tgaagttcgg cggcacgaac ggcatttgag ccggtcatgc
     7621 gcatgggccg ctcttgatgt gcaggagcgg cccgtggccg cgatgggctc gttggcgttc
     7681 ggcgcgattg gatcgcgcac aatggcaaaa gacggcgcta cgccccggca cgtccctaca
     7741 cgttgaaacg gaaatgcagg acgtcgcctt cctgcacgcg gtactccttg ccttccaggc
     7801 gcagccggcc ggcgtcgcgc gcgcctgatt caccccggta cttcatgtag tcgtcgaagg
     7861 aaacggtctc cgcacggatg aagcccttct cgaagtccgt gtggatcacg ccggctgcct
     7921 gcggcgcggt cgcgccctgc ttgacggtcc atgcacgaac ttccttgggg ccggcggtga
     7981 aatacgtctg caatccgagc aaggtgtatg cggcgcgaac cacgcggtcg aggccaggct
     8041 cgctcaatcc catcgaagcg aggaactcgc cgcggtcggc ctcgtcgagc tgcgccagtt
     8101 cttcctcgat cgcggcacaa accggcacca ccaccgcgcc ttcggtttcc gcgcgccggc
     8161 gcaccgcatc gagatgcgga ttgtcttcga agccgtcctc acgcacgttg gcgacgtaca
     8221 tcagcggttt aatcgtcaac aggaacaggt cgcgcatcgc ggcgcgttcc tcgtcgtcca
     8281 gcgccaccga gcgcgcggcc ttgccttggt tcaacgcggc ggcgagcttc tccagcaccg
     8341 ggcgcctggc catcgcttcc ttgtcattgg ccttggcggc gcgctcgacc cgcgccaggg
     8401 ccttgtccac cgattcgagg tcggccagcg cgagttcggt atcgatggtt tcgatgtcgg
     8461 cgatcgggtc gaccttgccg gtcacgtgga ccacgtcggg gtgctcgaag cagcgcacca
     8521 catgcgcgat cgcgtccacc tcgcggatgt gcgcgaggaa ctggttgccc aggccctcgc
     8581 ctttcgacgc cccggcgacg aggccggcaa tgtccacgaa ctcgaccgaa gccgggatga
     8641 ccttctgcgg gtgcacgatc tccgcgagtt gttgcaggcg cgcatccggc accggcacca
     8701 cgccgacgtt cggatcgatc gtgcagaacg ggaaattcgc cgcggcgata cccgccttgg
     8761 tcagcgcatt gaagagcgtg gacttgccga cattgggcag gccgacgatg ccgcattgga
     8821 tggccattcg gagctccggg aacagggaac agggaacgaa gaggcgggaa cggaacagat
     8881 gcgcaacgaa acgtccgcgc tacgttcccc gttccccgtt ccccgttccc gctgtttgag
     8941 tatgcagccg cttcatcgcc tcgttgaact cgcccgcgat ggtgagcggc aacacgttga
     9001 gcgcgcgcgc gatggcgtcc agcatcgcgt cctcgtctgc gcgcgaagga ttgccgagca
     9061 cccagggcgt cacgcggttg cggtcgccgg gatggccgat gccgatccgc aaccggtgga
     9121 acctgccgtg gccgagctgg gtcatgatgt cgcgcaggcc gttctggccg ccgtggccgc
     9181 cgtcgaactt caggcgcacc gtgccgggcg gaaggtcaag atcatcgtgc accaccagca
     9241 gctccgcggg cgggagcttc cagtagttga gtgcggcgac gatcgcgcgc ccgctttcgt
     9301 tcatgaaggt ggcgggcttg atcacgcgaa cctgctcgac gccgatcggc acgcggcatg
     9361 ccgctgcatg caggcgaccc tcggaggcga agcgcacgcc cgcgccgctc gccagtgcat
     9421 ccgcgaacca gaagcccgcg ttgtggcggt tgcgcaggta ttcggcgccg ggatttccga
     9481 ggccggcgat gaggcgtatg cccatgggcg cggaagcggg aaccgggaac gacaagccgg
     9541 aacggggaac aggaaatcac gcttccctgt tccccgttcc ctgttcccct tgttcctact
     9601 tcttttcttc cttggcgggc gcagcggcct tgacggcctc gccggacggt gcggcagcag
     9661 cggccgcgcc ttcggccggg gcgccctcgg cacccgcttc ggccggcgct tcctcgactt
     9721 cctcgcggac cgcctgcgcg gacacgacgg tgtggtcgta ttccttgccc agcttcagct
     9781 cggggatttc cacgccctcc ggaagcttca ggtccgacaa gtgccggctg tcgccgagct
     9841 cgaggtcggc gaggtccacc tcgatgaatt caggcaggtc cttcggcagg cacgacacct
     9901 cgacctcggt gaggttgtgc gaaatcacca cgcccgaagt cttgccggcg gcggacttct
     9961 ccttgttgat gaaccggatc gagacataca cgcggatcgg ctttgcctcg tcgatgcgca
    10021 ggaaatccag gtgcagtacc tgctccttga ccggatgctt ctggaagtcg cgcagcagga
    10081 ccttctggcg cttgccttcg accaggaggt cgaggatcga cgacgcgaac cagtcgtgcc
    10141 gcgatgcgag gacgacggtc tcgtgctcga actggacgtt ttccgcgtcc gtgcccgcgc
    10201 cgtacacgac gcccggaacc ttgcccgcgc gacgcaggcg gcggctcgca cctttgccct
    10261 ggtccgcacg acgctcggcc gtgatttcat ggatggttgc cattggttgt tacctcgggt
    10321 tgcatgggtc cgcctcgcgg cggatgaaaa ctctcccgcg accaggagag tcaatgaagc
    10381 gggaacaggg aaaggggaac ggggaaacga ggctgtcgtt ccaatccctg ttccccgttc
    10441 ccgcctttgt tcaatccacg tacaacgagc tgaccgattc gccatacgcc atccggcgga
    10501 cggtttcggc cagcaattgg gcgacggaca attgccggat cttgccgcag gcggcgcctt
    10561 cctcgctcag cgggatggtg tcggtcacga ccagttcgtc cataagcgat tgttcgatgt
    10621 tgcgcaccgc cgggcccgac agcaccgcgt gggtgcagta cgcgaccact tccttggcgc
    10681 cgcgatcctt gagcgccttc gccgcggcac acaaggtgcc ggcggtgtcg acgatgtcgt
    10741 cgaccagcac gcaggtcctg ccgacgacgt caccgatgat gttcatcacg gtcgcttcgt
    10801 tcggccgcgg ccggcgcttg tcgatgatcg ccagatctgc gtcgtccagg cgcttggcga
    10861 ccgcccgggc ccgcaccacg ccgccgacgt cggggctcac caccgtcacg ccctcggtgc
    10921 catgcttgcg ccagatgtcg gccagcaaca ccggcgaggc atacacgttg tcgagcggaa
    10981 tgtcgaagaa cccctggatc tggtcggcgt ggatatcgac tgtcagtacc gtatccgtgc
    11041 cgaccgcccc gatgagctgg gcggcgacct tggccgtgat cggcacccgc gccgcgcgca
    11101 gccgccggtc ctgccgcgcg taaccgaaat acgggatcac cgcggtcacg ctggcggcgg
    11161 aggcgcgctt cagcgcgtcg acgagcacca gcaattccat caggttttcc gcgaccggcg
    11221 cgcaagtcga ctgcatcacg aagacgtcct ggttgcggac gttctcgtcg atctcgatct
    11281 gcacctcgcc atcggagaac tggccgacga cggccttgcc cagcggctcg ccgagcgttc
    11341 gcgcgacatc ctctgccagc ttgcggttgg cgttgccact gaacaacaac aagctggatg
    11401 cgttgtgcat gctcaatacc ttcggatgca gccccacggg gaactggggc ggcaggattc
    11461 gaacctgcga atgccgggat caaaacccgg tgccttaccg cttggctacg ccccatcatg
    11521 tacagagcgg gcatcctgcc cggcgcaatg gataccgtcg cggccatgcc gcgccgaaat
    11581 accgcatcgg cgccgagcgc ctccgcgccg gtgcatccct gtgcctgccg ctgcgccttc
    11641 aagcggtgcg accgaacctc ggaatggccg cgcagaaaag ccgcatattg tcgcggaccg
    11701 gccaagccgc gtcaattcag cgttccgggt cgctccgccc atgcgcggcg gcggcccctc
    11761 ccgcggattc ggcactcgcg ccgttccatt cctggatcga cagccgcacg ctgtagggat
    11821 cgcggctggc gtacaccttg cgcggcagcg gcgggctgcg ctcggcatac cagtcgcggt
    11881 aatcgacgtg ccagccgtcc tggtccaggg tcgccggcag gccgttggcg ccgaaggtga
    11941 ggatcgcggg ggcgcccggc gcgcgcaggc cccgcaccca ccacgcgaga tccgccaccg
    12001 gcacgttcca gccgaattcc gcattcagca cctcgcccgc gttgcggccg gccagcggct
    12061 ccctgccggc gccggtcagc accgcgccgc cggggccacc gtcgagttcg accgtcttgc
    12121 cggtgatggg cgcgcgcaac gtgaaccggt acctgtcgcc ctgctggctc cagtccaggc
    12181 tgccgctgcc gccatgatcg ccatccgacg cggcgaaccg cgcgtgcagc ccccactgcg
    12241 cctgggcgcc cagcatccgt tcgcgcgcgg tctgcgccgc gagctgtgcg gcagtgccgg
    12301 gatggcgaac cggcaccggc gcgcaggccg caagcaccag gcctgccagc cccgctgcca
    12361 ccgcgagacg cagccaccgc gtcatgggcg caaccgtcgt tcggcgtgtt gcagcgccgc
    12421 gttgtgcgga tcgagcttgc gcaccttggc gaatacctcg cgcgcatccc ggtgttggcc
    12481 tagtttccac agcacctcgc ccaggtgcgc cccgatatcg gggtcctcgt ggctggccca
    12541 ggcgcgctgc aggtagctct ccgcttcatc caggtggccc agccgatatt gcagccagcc
    12601 ccaggaatcc atgatcgcgg ccgcgtccgg cttggcggcc agtgccttcc gcagcaggcg
    12661 ggtggcctcg gggaggtcgc ggtcggcgtc agccagggtg aacccgagcg cgttcatcgc
    12721 ctcgatgttt tcggggttcc gcgccagcac ctcgcggaag tcccggagcg ccgcatcggt
    12781 gttgcccgca ttggcttcct cgatgccgcg gtcatagatg agcgccgggt cgcccggcag
    12841 ggcgccgagc ccgcggtcgt ataccgcgat ggcgcgggcg tggtcgccgt gttgggcgta
    12901 caactgggca tccagctcgt acgccgtgcg caggctgtcg gggtcatcgg cgtaatcctg
    12961 ctggagctgc gccgccagcg cgtgtgcctg cgcctgcttt ccggtcttgt cgagcagcac
    13021 cgcactgcgc accttcgcct cgaaaccatg ttcgctgtcg acatcgacct gcccgtacca
    13081 atcgagagcc tgcgagtcgt ggtgcatcag ttccgccagt tgccccagca ggaaagcgtt
    13141 gtcggcacgc tggcccgggg gcgaccgctt cagttcggca tagaggcgct gcaatgcggc
    13201 gtcatccttg gcgcgcgccg cgaatgcgac gcgcgcgctc cacgtccgcg gcgtttgcgg
    13261 accgtcggcg agcacccgca gcgcctcgcc gtactcgtgc gcagcgccca gcacgctcgc
    13321 gtagccgagc ctcagcgccg gatcgcgcgg atgcgccgcg acgcccttcg cgtacagggc
    13381 cctggccccg gcgctgtcgc cggtcgacag cttcaggctg gccgcccagc ggatactgtc
    13441 ggttcccccg aagcgttgta ccgccgcgtc ggccagcttg cgcgcgaagg cgtggcggcc
    13501 gagatgctcg cccagctggc tcagcgccac ccacacgttt tcgttgcgcg ggaggcgctc
    13561 cgccaccgca agatcttcca ggatcctgcc cgcgagcgcg gtatctcgcg ccgccagcag
    13621 gtctgccgcg aaggttttcc aggcatcggc gccgcccgtc gcgagcagga tgtcgaagtc
    13681 cttttcggcg ccggaacgat cgccccgcag catggccagt tgcgcgcgga tgccggccag
    13741 gtcgcgcgga gcggcgccga gtgcccgcca acggtccagc agctttttcg catttgcggt
    13801 ttcacccgag gccaccgcga gctgcagcgc gcgcttcgcc accgcgggat cgtccgcgat
    13861 ctccgcggcg gccgcgtagt cccgcgccgc ctcgtcgatg tcctggtcct gcagcgcaaa
    13921 ttcggcggcg atcagcaggg cggacgcctt ggcggccggc ccggagggct cgatgtcgag
    13981 ccgcgccagg ggctgcgcct cggcaacggg cccgtaaccc gtgggctgcc gtgaaccggc
    14041 gcgcggggac agcggcgcgc aacctgcgag ccaggccacg gcgagcacca cggcaggcgc
    14101 gctaaaatga aaccacgacg gcttcatcct ggccaggaca cggatgggcg cccagccggg
    14161 atccagtttc ttgaatttgt cgggttgggt ccgcacaatt cccacgccgg aaaatccggt
    14221 ccgctccatt tcgcctccag acgacagctt agcctgtcgc cgaacccgtt cccatgccgc
    14281 tgatcgcact cggactcaac cacctgaccg cgccggtctc gttgcgcgag caggtggcga
    14341 tcgacgcgga ggccacggcc ccggcgctcg ccgacctcgc atcccaggcc ggcgtcgacg
    14401 aggcgctgat cctgtccacc tgcaaccgca cggaactgta ctgcagcgtc cgcgacggcg
    14461 ccgagggcgc gccatcggcc tggttgcaca cccacaacca gatgaccccg gggcgcctcg
    14521 acgagttcct gtaccggcac accgatggcg acgccgtgcg gcacgtgttc cgggtcgcga
    14581 ccggcctcga gtcgatggtg ctcggcgagc cgcagatcct cggccaggtg aaggacgcct
    14641 ggcgcgcggc gcggtcggcg cgcaccctgc aggcgccgat ggaccggctg ctgcagaacg
    14701 ccttcacggt cgccaagcgg gtgcgcaccg agacccgcat cggcagcggt ccggtctcgg
    14761 tcgcgttcgc cgcggtgcgg ctggtcgagc aggcgttcgc ggacctgcgc gaatcctgcg
    14821 tgctgctgat cggcgcgggc gacaccatcg aactcgcgat caggcacttg atcgagcgcg
    14881 gcgtgcggcg cctgatcgtg gccaaccgca ccatcgaaac agcgcaggca ctgatcaatc
    14941 cggtgggcgg ctacgcgatc gcgctggccg acttgccgaa gcacctcgcc gaggccgaca
    15001 tcgtgatgtc ggcgaccgcc agccgggttc cgatcctgga tgcaccgatg ctgcgcgggg
    15061 cgctgcgcca acggcgccgg cgcccgatgc tgctgatgga cctcgcggtg cctcgggaca
    15121 tcgaaccttc ggtggccgaa ctgcccgacc tgttcctgta cacgatcgac gacctccagc
    15181 aggccgtcga cgtcgggcgc cgctcgcgcg aggcgtcggt gcgtgaagcc gaggcgatca
    15241 tcgacctgca ggtcgagcgt tacctcgcgt ggcgccgcgc caaccagttc gaccagccgc
    15301 tgcgctgcta tcgcgcgacc gccgaagccc agcgcgacga ggtgctggca cgcgcgcgcg
    15361 agatgctggc cgcgggccgc gctcccgacc aggcgctgga gtacctcgcg aacaccctca
    15421 ccggcaggct gctgcacacc ccgagcgtga aactccatga agccgccgag catggcgacc
    15481 aggccctgct cgacgcggcc gcgcgcctgt tcgacatgaa cgggcgcgat gagtgaaccc
    15541 gcaactgaag cgccgcctcg aacaactgct ggaacgccac cacgaggtcg ggctactgtt
    15601 ggcggagccc ggagtcgcca acgacccggc gcatttccgc gagctgtcgc gcgaatacgc
    15661 gcagacgcag gaactcgccg aagtcttcgc cgaatcgcaa aagggcgaag ccgaactcgc
    15721 gcatgcacgc gcgatgctcg ccgatcccga cctgcgcgaa ctggcgcgcg aggaagtcgc
    15781 acggctggag caatccagtg cccacctcga ctcccgcctg caggcgctgt tgacgccgcg
    15841 cgacccgctg gacgacgcca acctgttcct cgaagtgcgc gcgggcaccg gcggcgacga
    15901 ggccgcgctg ttcgcgggcg acctgctcag gatgtacctg cgctacgccg agcgccgtgg
    15961 ctggcgtgcc gaattgatgt cggcccacga gggcgaacac ggcggctaca aggaagcggt
    16021 cgcgcgtatc gagggcagcg gcgcgtatgc cgcgctgaag ttcgaatccg gcacccatcg
    16081 ggtgcagcgc gtgcccgtca ccgaagcttc gggtcgcatc cacacctccg ccgcgacggt
    16141 ggcgatactg cccgagcagg cggaaatcgc cgatgtcgaa ctccgtgacg ccgacctcaa
    16201 ggtcgacacc ttccgcgcct ccggcgccgg cggccagcac gtcaacaaga ccgactcggc
    16261 gatccgcatc acccacctgc cgaccggcat cgttgtcgaa tgccaggacg agcgcagcca
    16321 gcacaagaat cgtgcgcgcg cgttgtcgct cctgaaggcg cggctgctgg acgcgcaacg
    16381 ctcggaacag accgcgcagc aggccgccac gcgcaaactg caggtcgggt cgggcgaccg
    16441 cagccagcgc atccgcacct acaacttccc gcaaggacgc gtcaccgacc accgcatcaa
    16501 cctgaccctg caccagttgc cgcagatcct cgacggggac ctcgacgagc tggtcggggc
    16561 actggccagg gaatcgcacg ccgaagcact cgcggcgctc ggcgaggcgc catgaacgac
    16621 gcgcgcaacg cggatctccg cacacgcctc gcgaacatcg ccggattgct cgaacgccgc
    16681 gaagcggccg gcgcagaacg cctcgccgcc gacgcccgtg ccgcgcaccc gcaggcttcc
    16741 gaagccgcgc gccttcacgg catcgcattg ctgatgctgg atcgtgggac cgaggcacgc
    16801 gatgccctgg aaacggcgct ccgcctgcaa ccggacagcc cggaagctgg cagcaacctc
    16861 gccagcgcct tgctcgcgct cggcgatgcc gccggcgcgg tggccttgct ggaacgagtg
    16921 gtgggggcgc ggcccaacga tcctgcgctc cgcaacaatc tcgcgaacgc cctgcgcgcg
    16981 gccggggacg acagccgcgc gcgcgaggaa tacctggcgg cactcgccgc ggcacccggt
    17041 catttcgggg cgagcgccaa tctcgctgcc gtcgagattt cgctgggcat gctggacgag
    17101 gcggaggcgc gattgcgttc cctgctcgcg gcccagccgc atccgcaact gtacctgttg
    17161 ctcggccacg tcctgcatcg ccgccatcgt ttcgcagacg cgcaggcggc gtacctgcaa
    17221 ggagcccagc tcgcccccgg cgctgcggaa ctcgcctacc aggcggccct gatggcggac
    17281 gagcagcacc actacgcgga agctgcc
//
LOCUS       DASSNH010000176        10305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_948408, whole genome shotgun sequence.
ACCESSION   DASSNH010000176 DASSNH010000000
VERSION     DASSNH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948408"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..2904)
                     /gene="lon"
                     /locus_tag="VFK00_06610"
     CDS             complement(385..2904)
                     /gene="lon"
                     /locus_tag="VFK00_06610"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7592655.1"
                     /translation="MPNEIPESLSAIVELPVLPLRDVVVYPHMVIPLFVGREKSMKAL
                     EAAMEGDRRILLVAQKQPEIDDPGEDDLNVVGTIGSLLQLVKLPDGTVKVLVEGQSRA
                     QVQRSFETDGMLRADAVSLQPVFDRPASELETAARMLVGLFEQLVRQSRKLPPEVLTG
                     LSGIDDLSRLADSVAAHLSVRLSDKQKVLDTLAVGARTEMLIGLVESELDLQQVEKRI
                     RGRIKTQMEKSQREYYLNEQMKAIQKELGDIEEGGNEIDALAKKIEGAGMPKTVLAKA
                     KGEFNKLKQMPPMSAEATVVRNYLDWMTSVPWKKRSKVRKDLRVAEQVLDEDHYGLEK
                     VKERILEFLAVQQRVNKIKGPILCLVGPPGVGKTSLGHSIARATNRKFVRQSLGGVRD
                     EAEIRGHRRTYIGSLPGRIIQNMAKVGTRNPMFMLDEIDKMAMDFRGDPSAALLEVLD
                     PEQNHAFNDHYLEVDFDLSEVMWIATANSMNIPAPLLDRMEVIRIPGYTEDEKVAIAT
                     KYLLPKQIRGAGLKESELSVSEGSLRDIIRYYTRESGVRNLERELAKICRKVVKELTL
                     AETGKPEKRPAEAADQGKPKKAATRKRGAAVKVESRNLDKYLGVRRFDFGRKEDQNEV
                     GMVTGLAWTSVGGDLLNIEATVVPGKGKLILTGQLGDVMKESIQAAQSVVRARSQLLG
                     ISADFHDKYDIHVHVPEGATPKDGPSAGIAMATALVSALTKDPVRAEIAMTGEITLRG
                     RVLPIGGLKEKLLAAHRGGITTVIIPEENKKDLADIPKNITDALTIHPVRWIDQVLEL
                     ALEHPLTPLPANADPVATETGKLAPENARESGEEGGAARTH"
     gene            complement(3259..3891)
                     /gene="dksA"
                     /locus_tag="VFK00_06615"
     CDS             complement(3259..3891)
                     /gene="dksA"
                     /locus_tag="VFK00_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HET7592656.1"
                     /translation="MSRQSSIPKAVSVPESPVVSKADVAAQESRHRSAPRKRVHQRPT
                     GGVAARLSAGGGDEQTITREDGRYALPATVTIDLPKGYRPSAKEEYMSPRQLAYFRQK
                     LKDWRDQLVEESRQTMENLREEVRDVGDDAERATRETENSLELRTRDRYRKLISKIEK
                     ALRKIEEGRYGYCEETDEEIGLERLEARPIATLSLDAQERREHLQKQMGD"
     gene            3902..4318
                     /locus_tag="VFK00_06620"
     CDS             3902..4318
                     /locus_tag="VFK00_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592657.1"
                     /translation="MAARDVVVFLVACGLDFFAAAVLDVFLTGDDLNDFFTMDDLVDF
                     FAAGDLADFFTAGDFVTFFAATDLRLAGAGRAFLAAGLDAVLRIGFAAAFAACILLAG
                     LAVWPAFLSLRLAAPCLDLAMSLHSRVVEMRPRVIA"
     gene            4440..4637
                     /gene="yidD"
                     /locus_tag="VFK00_06625"
     CDS             4440..4637
                     /gene="yidD"
                     /locus_tag="VFK00_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7592658.1"
                     /translation="MLGARCRFHPSCSTYARVAIARFGPLRGGILATWRILRCQPLCE
                     GGFDPVPESFTLRRCRSHGGH"
     gene            4638..5972
                     /locus_tag="VFK00_06630"
     CDS             4638..5972
                     /locus_tag="VFK00_06630"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7592659.1"
                     /translation="MADWLIKHAELVNEGRRFEADLRIRGHRIAEIGSGLAAHGGEQI
                     FDAAGLWLLPGVIDDHVHFREPGYTAKADIASESRAAVAGGVTSFFEMPNTRPATLTR
                     ELLEDKYEIASRTSLANYAFYFGASNDNLDAIRALDPREVPGVKVFLCESTGNLCVSD
                     PSTLEGVFRDSPVTVLVHAEDSRIIHANLDRARAEYGDDIPMRQHPEIRSREACFEST
                     RWAVDTARRHDTRLHVLHVSTADELALFEQGPTADKRITAETCVHFLRFSDAEYATLG
                     GKIKCNPAIKATTDREALIRALRDGRIDILATDHAPHLESEKANPYTRCPSGIPLIQF
                     ALQSSLEHVLDGRLTLERLVEATSHAPATLFAIRERGYLREGWFADLVLVDPATPHTV
                     RREDIVSKCGWSPFEDDTFRASIAATFVNGNLAARQGKVLESGPGMRLAFDR"
     gene            complement(6125..7063)
                     /gene="folE2"
                     /locus_tag="VFK00_06635"
     CDS             complement(6125..7063)
                     /gene="folE2"
                     /locus_tag="VFK00_06635"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HET7592660.1"
                     /translation="MNPDSDNARLPDVASGPEPATGGALDWVGMEGIDLPVRVADGQG
                     GIATAPAKVGVQVDLADPAARGIHMSRLYIALEQALAGQALTPSGLRSLLHALVASHA
                     GLSTRVRLRIGYAQLLKRRALASGNAGWRSYPVMVEAQLRDGRFNVKLGCEVTYSSTC
                     PASAALSRHLNRQRFDEDFADVAPTHRAVGEWLDGERGMAATPHAQRSTARVTACLTA
                     GFDLPVAELIDMVEGALATPVQAAVKREDEQAFAKLNAENLMFCEDAARRVRAALDAD
                     ERIAGYRVVATHHESLHAHDAVAVASRNFPADEERS"
     gene            complement(7073..7909)
                     /locus_tag="VFK00_06640"
     CDS             complement(7073..7909)
                     /locus_tag="VFK00_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET7592661.1"
                     /translation="MSDSALDSYVSSWRDADPQRAVAWLFLRPAERIRYGACAALLQE
                     WRKTLREVSEPQVAAAKLGWWREELQRAAEGEARHPLAQALFADPRVREVPLPCWTAV
                     LDAAFGQVSAPPPSDFPAQQQAAEPWSIAVAALESRLWFGPWADTARAAAVTRVTELV
                     ADLRAMGSAAVNGRSPLPMNLLARHGITIEGLTGDSPSRRAALRDYSNLLREALTEAA
                     GMPGPLTLFRAACMQRDLPALERASRAEDPLAAVCAQRAGLGQLLKTWRAARTWRGSA
                     KT"
     gene            complement(7906..8571)
                     /locus_tag="VFK00_06645"
     CDS             complement(7906..8571)
                     /locus_tag="VFK00_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7592662.1"
                     /translation="MKPLPRPLAGVFFDLDGTLVDSARDMFDALAMLCEEQGRQPPAF
                     DTVRETVSRGARAVLRCAFGGDELAIDAVLPRFLELYVGTGMRHTHAFPGVEALLDKL
                     EAREVRWGVVSNKASHLVALVLAALGYDQRVNAVVGGDTLPQRKPDPAPVRHACELAG
                     VMPGQCVFVGDDPRDIEAGREAGLYTVAAAWGYLDGADPKEWRPDAIAADADQLAGWL
                     DQA"
     gene            complement(8568..9299)
                     /gene="ubiG"
                     /locus_tag="VFK00_06650"
     CDS             complement(8568..9299)
                     /gene="ubiG"
                     /locus_tag="VFK00_06650"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HET7592663.1"
                     /translation="MNVASHNADPEELARFGKLAHRWWDPDGESRPLHDLNPVRLAFV
                     AARVALHGARVADVGCGGGLLSEALAKAGAAVTAIDLAPEVIEVARLHLHETNDSMPQ
                     PLEIDYRLSSSRELAASEPGAFDAVCCMELIEHVPDPAALVDDLAALLRPGGKLFLST
                     LNRTPLSFATAILGAEYIAGLLPRGTHDYRQFLRPSELATLLRHSGLGLEEISGIGYN
                     PLARKAWLTRRTAVNYLVCAAKPAA"
     gene            complement(9296..9574)
                     /locus_tag="VFK00_06655"
     CDS             complement(9296..9574)
                     /locus_tag="VFK00_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7592664.1"
                     /translation="MKRYLVLTLRTPRFDPAVIEAHYSHLDALKARGALEAFGPFADR
                     SGGAYLVRAESLEEARGMAFADPVHVTGSSQVSVYEWDLRPVEQVQAA"
     gene            complement(9588..>10305)
                     /locus_tag="VFK00_06660"
     CDS             complement(9588..>10305)
                     /locus_tag="VFK00_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7592665.1"
                     /translation="FERIRMLSDQLDVPVHLHLMETAQEVEDSRREHGLRPMQRLQKL
                     GLVNDRLIAVHMTQVSDAEIAACAEAGVSVVHCAESNLKLASGFCPAEKLRRAGVNLA
                     LGTDGCASNNDLDMFGELRTAALLAKAVAGDAAAFDDAFALRAATLGGACAMGLQAAI
                     GSVEPGKRADLAAVRMDEVETQPLYNVVSQLAYAVSRRQVSDVWIDGHRKLGNGELVD
                     FDLAGVLERARRWRERIIAL"
BASE COUNT         1605 a         3522 c         3360 g         1818 t
ORIGIN      
        1 tcgagtgcac gtccggcgtc ggctttcgac agatcggcgt ggtcggcaat tgcattgatc
       61 aattcagctt tgttcatgga gactccctct tgggtttaga gcagcccgcg gaatgttcgg
      121 cggaaagacg cgtcccccaa aaatgcgaaa gcgtccggtc cggtcgtgtt gcaacccgtt
      181 tcgatacgcg gggatcgact cgcgcgtctt acggcccggt ctttatacca gccccccaga
      241 gggcgtgcaa taaaggcggg gcttggattt cagcgcttcg gcacgcgcgc ggaaaaacat
      301 ttcggcgcgc gatcatgccg cgcgccgaag cgcaagtgcc gaatgaaagg aatgcgcgat
      361 cccgttggcg atcgccgcac gggatcagtg ggtgcgtgcc gcaccgccct cctcgcccga
      421 ttcgcgcgcg ttctcgggag cgagcttccc ggtctccgtc gccaccggat cggcgttcgc
      481 cggcagcggg gtcaacggat gttccagcgc gagttccagt acctggtcga tccagcgcac
      541 cgggtggatc gtcagcgcgt cggtgatgtt cttcgggatg tcggcgagat ccttcttgtt
      601 ttcttccggg atgatcacgg tggtgatgcc gccgcgatgc gccgcgagca gcttttcctt
      661 cagcccgccg atcggcagca cccggccacg caaggtgatc tcgccggtca tcgcgatctc
      721 ggcgcgcacc ggatccttgg tcagcgccga caccagtgcc gtcgccatcg cgatgcctgc
      781 gctcggacca tccttgggcg tcgcgccttc cggcacgtgc acgtggatgt cgtacttgtc
      841 atggaagtcc gccgagatgc cgagcaactg gctgcgtgcg cgcaccaccg actgcgcggc
      901 ctggatcgat tccttcatca cgtcgccgag ctggccggtg aggatcagct tgcccttgcc
      961 gggcaccacg gtggcttcga tgttgagcag gtcgccgccg accgacgtcc acgccagccc
     1021 ggtgaccatg cccacttcgt tctggtcttc cttgcggccg aagtcgaagc gccgcacgcc
     1081 gaggtacttg tcgaggttgc gcgactcgac cttgaccgcg gcgccgcgct tgcgggtggc
     1141 tgccttcttc ggcttgccct ggtccgccgc ctccgccggt ctcttctcgg gcttgccggt
     1201 ctccgcgagg gtcagctcct tgaccacctt gcggcagatc ttcgcgagct cgcgctccag
     1261 gttgcgcacg cccgattcgc gcgtgtaata gcggatgatg tcgcgcagcg aaccctccga
     1321 aaccgagagt tcgctctcct tcaggcccgc gcccctgatc tgcttgggca gcaggtactt
     1381 ggtcgcgatc gcgaccttct cgtcctcggt gtaaccgggg atgcggatga cctccatgcg
     1441 gtccagcagc ggtgccggga tgttcatgga gttcgcggtc gcgatccaca tgacctcgga
     1501 cagatcgaaa tcgacttcga ggtagtggtc gttgaacgcg tggttctgct cgggatcgag
     1561 tacttccagc agcgccgccg aagggtcgcc gcggaagtcc atcgccatct tgtcgatctc
     1621 gtccagcatg aacatcgggt tgcgggtccc gaccttcgcc atgttctgga tgatccggcc
     1681 cggcagcgac ccgatgtagg tgcggcgatg gccacggatc tcggcctcgt cacgcacgcc
     1741 gccgaggctc tggcgcacga acttgcggtt ggtggcgcgg gcgatcgaat gcccgagcga
     1801 ggtcttgccg acgccgggcg ggccaaccag gcacaggatc gggcccttga tcttgttgac
     1861 ccgctgctgc accgcgagga actccaggat gcgttccttg accttctcaa ggccgtaatg
     1921 gtcttcgtcg agcacctgct cggccacgcg gaggtccttg cggaccttgc tgcgcttctt
     1981 ccacggcacg ctggtcatcc agtcgaggta gttgcgcacc acggtggctt ccgccgacat
     2041 cggcggcatc tgcttgagct tgttgaactc gcccttggcc ttggcgagca ccgtcttcgg
     2101 catgcccgcg ccttcgatct tcttggccag cgcgtcgatc tcgttgccgc cctcctcgat
     2161 gtcgccgagc tccttctgga tcgccttcat ctgctcgttg aggtagtact cgcgctggct
     2221 cttctccatc tgggtcttga tccgcccgcg aatgcgcttc tcgacctgct gcaggtcgag
     2281 ttcgctctcg acgaggccga tcagcatctc ggtgcgcgcg ccgaccgcga gggtgtccag
     2341 caccttctgc ttgtccgaca ggcgcaccga cagatgcgcg gcaacggaat cggccagccg
     2401 cgacaggtcg tcgatgccgg acaggcccgt cagcacctcc ggcggcaatt tgcggctctg
     2461 ccgcacgagc tgctcgaaca gtccgaccag catccgcgcg gcggtctcga gttcgctcgc
     2521 cggacgatca aagaccggct gcagcgacac ggcatcggcg cgcaacatgc cgtcggtctc
     2581 gaaggaccgc tgcacctgcg cgcgcgactg cccttcgacc agcaccttca cggtgccatc
     2641 gggcaacttc accagttgca gcaggctgcc gatggtgccg accacgttca ggtcgtcttc
     2701 gccggggtcg tcgatctccg gctgcttctg cgcaaccagc aggatgcggc gatcgccttc
     2761 catcgccgct tccaacgcct tcatcgactt ctcgcggccg acgaacaacg ggatcaccat
     2821 gtgcgggtac accacgacgt cgcgcagcgg cagcaccggc agctcgacga tcgcggacag
     2881 ggattcgggg atttcgtttg gcatgtcgat ccggctcagg attgcgcaaa ggcccgcaat
     2941 gcgggcgatg gaatcaggat ggtggcggcg cgcgcccgga tcaagcggcc gagggtgacg
     3001 cggctcgcaa acgcggatat gaacggttcc acccgctgca cgagcgagcg gccggggcgc
     3061 cggagcattt gcaggccggg ttgagcgcag cgaagcccga cgcgcgggac gggctgccgt
     3121 cgccgtcgcg tgcgaaaaaa aggcggcgct tcgtgccgcc gcgatcctgc aaaagaagcc
     3181 gcggatcggg tgcgatcctg cgactgccgt tgcaggagcg cgccgtgcgc gcgaccgcgt
     3241 tagcggaacg aacgcgctct agtcgcccat ctgcttctgc agatgctcgc ggcgttcctg
     3301 ggcgtccagc gacaacgtcg cgatcggacg cgcctcgagg cgttcgaggc cgatttcctc
     3361 atcggtctct tcgcaatagc cgtagcggcc ttcctcgatc ttgcgcagcg ccttttcgat
     3421 cttggagatc agcttgcggt agcggtcgcg ggtacgcagc tccagcgagt tctcggtttc
     3481 ccgggtcgcg cgctcggcgt cgtcgccgac atcgcggact tcctcgcgca ggttctccat
     3541 cgtctgccgc gattcctcga cgagctggtc acgccagtcc ttcagcttct ggcgaaagta
     3601 cgccaactgc ctcggactca tgtattcctc tttggcgctg gggcgatagc ccttcggcag
     3661 gtcgatcgtc acggtggcgg gcaacgcata gcgaccgtct tcccgcgtga ttgtctgttc
     3721 gtcgccaccg ccggcgctga ggcgcgcggc gacgccgccg gtcggccgct ggtgcacccg
     3781 cttgcgcggc gcggaacgat ggcgggactc ctgggccgcg acgtcggctt tcgacaccac
     3841 cggcgattcg ggcacggaaa cagccttcgg aatcgacgac tggcgggaca ccgcggcctt
     3901 cttggccgcg cgcgacgttg tcgtcttctt ggtggcctgc ggcctggact tcttcgccgc
     3961 ggcggtgctg gatgtcttct tgacggggga tgatttaaat gatttcttca caatggacga
     4021 cttggttgat ttcttcgctg caggtgactt ggcggatttc ttcacggcgg gcgactttgt
     4081 cacctttttc gcggcgaccg acttgcgctt ggccggcgcc gggcgggcct tcttggccgc
     4141 cggtctggac gcggtcttgc ggatcggttt cgcggccgct ttcgccgcgt gcatcttgct
     4201 ggccggcttg gcggtgtggc ccgctttttt gtcgctgcgc ttggcggcgc cgtgtttaga
     4261 cttggccatg tccctgcact cgagagttgt ggagatgcga ccgcgtgtta tagcgtagtc
     4321 gtttcgaccg ggcaaccgat ttcatcgccc gccgggcaaa cccgttcatc ctaggcggca
     4381 ccgccttgac ccgcgtcctg ctgttgctca tcacgttcta caagcgcttc ctgagtccga
     4441 tgctcggcgc gcgctgccgc ttccatccaa gttgttcgac ctacgcccgc gtcgcgatcg
     4501 cgcgtttcgg ccccttgcgc ggcgggatcc tggcgacctg gcggatcctc aggtgccagc
     4561 cgctgtgcga gggcggcttc gatccggtgc ccgaatcgtt tacgctgcgc cgctgccgct
     4621 cgcatggagg acactgaatg gccgactggc tgatcaagca cgccgaactg gtcaacgagg
     4681 gccgcaggtt cgaggccgac ctgcgcatcc gcggccaccg gatcgcagaa atcggcagcg
     4741 gcctggcggc gcacgggggt gaacagattt tcgatgccgc cggcctctgg ctgctgcccg
     4801 gggtgatcga cgaccacgtg catttccgcg aaccgggcta taccgccaag gccgacatcg
     4861 cctccgaatc acgcgccgcg gtcgcgggcg gcgtcaccag cttcttcgaa atgccgaaca
     4921 cccggcctgc gaccctgacg cgcgaactgc tggaggacaa atacgaaatt gcgtcgcgca
     4981 cctcgctcgc caattacgcg ttctatttcg gggccagcaa cgacaacctc gatgcgatcc
     5041 gcgcactgga tccccgcgag gtgccgggcg tcaaggtgtt cctgtgcgaa tcgacgggca
     5101 acctgtgcgt cagcgaccct tcaacgctgg agggcgtgtt ccgcgattcg ccggtcacgg
     5161 tgctggtgca tgccgaagac agccggatca tccatgccaa cctcgatcgg gccagggccg
     5221 aatacggcga cgacattccg atgcggcagc acccggagat ccgctcgcgc gaagcctgtt
     5281 tcgaatcgac gcgctgggca gtggacactg cccgccgcca cgacacgcga ctgcacgtcc
     5341 tgcacgtttc caccgccgac gagctcgcgc tgttcgagca aggtccgacc gcggacaagc
     5401 gcatcaccgc cgagacgtgc gtgcatttcc tgcgtttcag cgacgcggag tacgcgacgc
     5461 taggcggcaa gatcaagtgc aacccggcga tcaaggcgac gaccgaccgc gaagccttga
     5521 tccgcgcgct gagggacggc cgcatcgata tcctcgccac cgaccatgcc ccgcacctcg
     5581 agtcggaaaa ggccaatccc tacacccgtt gcccgtccgg catcccgctg atccagttcg
     5641 ccctgcagtc gtcactggaa cacgtgctgg atggacggct gacgctcgag cggctggtcg
     5701 aggcgacgtc gcacgcaccg gcgacgctgt tcgcgatccg cgagcgcggc tatttgcgcg
     5761 agggctggtt cgccgacctg gtgctggtcg atccggcgac gccgcacacc gtgcgtcgcg
     5821 aagacatcgt ctcgaaatgc ggctggtcgc cgttcgaaga cgacactttc cgcgccagca
     5881 tcgccgccac cttcgtcaac ggcaacctcg ccgcgcgcca gggcaaggtg ctggaatcgg
     5941 ggccggggat gcgtttggcg tttgaccgtt gaatccggaa atctcttgac gcggataaac
     6001 acggataagg gcggatagaa ccatccgatt ttctgatgcg cgccatgtcg gcaagcgcgt
     6061 cttttgccga gatgcggcgc caatcagaaa tgcttttatc tggattgatc cgcgttcatg
     6121 cgtgtcaaga acgctcttca tccgccggga agttccggct cgccaccgcg accgcatcgt
     6181 gcgcgtgcag gctttcgtgg tgggtcgcga cgacgcggta gccggcgatg cgttcgtcgg
     6241 catccagcgc ggcgcgaacg cggcgcgcgg cgtcctcgca gaacatcagg ttctcggcgt
     6301 tcaacttcgc gaaggcctgt tcgtcctcgc gcttgaccgc ggcctgcacg ggcgtggcga
     6361 gcgcaccctc caccatgtcg atcagttcgg cgaccggcag gtcgaagccg gccgtgaggc
     6421 atgccgtgac gcgtgcggtg ctgcgctgtg catgcggcgt cgcggccatc ccgcgctcgc
     6481 cgtccagcca ttcgccgacg gcacgatggg tgggagccac atcggcgaaa tcctcatcga
     6541 agcgctgccg gttgaggtgc cgcgacagcg cggccgaggc gggacaggtc gaggaatacg
     6601 tgacctcgca accgagtttc acgttgaagc gcccatcccg caattgggct tcgaccatga
     6661 ccggataact gcgccagccg gcgttgccgc tggcgagggc acggcgcttc agcaactgcg
     6721 catagccgat gcgcagccgc acccgggtgg aaagacccgc gtgcgaagcg accagcgcgt
     6781 gcagcaagct gcgcaacccg gacggcgtca acgcctggcc ggccagcgcc tgctcgaggg
     6841 cgatgtacaa ccgcgacatg tggatcccgc gtgcggcggg atcggcgagg tcgacctgca
     6901 ctcccacctt cgccggggcc gtcgcgatgc caccctgccc gtccgcgacc cgcaccggca
     6961 ggtcgattcc ctccatcccg acccagtcga gcgcgccacc cgtggccggt tccggcccgc
     7021 tggcgacatc gggcaggcgt gcgttgtcgg aatcaggatt catcatgcgg cctcaagttt
     7081 tggctgaccc gcgccacgtg cgggccgcgc gccaggtttt caacaactga cccagaccgg
     7141 cccgctgcgc gcacaccgcc gccaggggat cttccgcacg cgaggcccgc tccaatgcgg
     7201 gcaggtcgcg ctgcatgcag gccgcgcgaa acagcgtcag cggcccgggc atgccggcag
     7261 cctccgtcag cgcctcacgc aacaggttcg aataatcgcg cagcgctgcc cggcgcgaag
     7321 gcgagtcgcc ggtcaatcct tcaatcgtaa tgccgtggcg cgccagcagg ttcatgggca
     7381 acggcgaacg gccattcacc gctgcgctcc ccatggcgcg aagatcggcg acgagctccg
     7441 tcacccgggt caccgcggcg gcgcgcgccg tatccgccca cggtccgaac cacaggcggg
     7501 attccagtgc cgcaaccgcg atggaccacg gctcggctgc ctgttgctgt gccgggaaat
     7561 ccgagggcgg cggcgccgag acctggccga atgcggcatc cagcacggcc gtccagcacg
     7621 gcaatggcac ttcgcgaacc cgtgggtcgg cgaacagcgc ctgtgccagg gggtggcgcg
     7681 cttcgccttc ggccgcgcgc tggagttcct cgcgccacca acccagcttg gccgcggcaa
     7741 cctgcggctc gctcacctcg cgcagggtct tgcgccactc ctggagcagc gccgcgcacg
     7801 ctccgtagcg gatgcgctcg gccgggcgca ggaacagcca cgccacggcc cgctgcggat
     7861 cggcgtcgcg ccaggacgac acgtagctgt cgagcgcgct gtcgctcacg cctggtccag
     7921 ccaacccgcg agctggtccg cgtcggcggc gatggcatcg ggccgccatt ccttcggatc
     7981 ggcgccgtcg agatagcccc acgccgctgc gaccgtgtac agccctgcct cgcggccggc
     8041 ctcgatgtcg cgcggatcgt cgccgacgaa cacgcactgc cccggcatca caccggccag
     8101 ttcgcaggca tgcctaaccg gtgcgggatc cggcttgcgc tgcggcaagg tgtcgccgcc
     8161 cacgaccgcg ttgacacgct ggtcgtagcc gagcgcggcg agcaccagcg ccaccaggtg
     8221 gctggccttg ttgctgacga cgccccagcg gacctcgcgc gcctcgagct tgtccagcaa
     8281 ggcctcgacg ccggggaatg cgtgggtatg gcgcatcccg gtgccgacat acagttccag
     8341 gaaacgcggc agcaccgcat cgatcgccag ttcatcgccg ccgaacgcgc agcgcagcac
     8401 ggcgcgtgcg ccgcgcgaca cggtttcgcg gacggtatcg aacgccggcg gctgcctgcc
     8461 ctgttcctcg cacagcatcg ccagtgcgtc gaacatgtcg cgggcggaat ccaccagggt
     8521 tccgtcgagg tcgaaaaaga ccccggcgag cggccgcggc aacggtttca tgcggcgggc
     8581 ttggccgcgc agaccagata gttgaccgcg gtgcgccggg tcagccacgc ctttcgcgcc
     8641 aatgggttat agccgattcc cgagatttct tcgagcccga ggcccgagtg gcgcaacaac
     8701 gttgccagct cggacggccg caggaactgg cgatagtcgt gggtgccgcg cggcaacagg
     8761 ccggcgatgt attcggcgcc gaggatggcg gtcgcgaagc tgagcggcgt gcgattcagc
     8821 gtcgagagga acaacttccc gcccggccgc aacagcgccg ccaggtcgtc gaccagcgct
     8881 gcgggatccg gcacatgctc gatgagttcc atgcagcaga ccgcatcgaa cgcgccgggc
     8941 tcggaagccg cgagctcgcg cgacgaactg aggcggtaat cgatctcgag cggctgcggc
     9001 atggaatcgt tggtttcgtg caggtgcagc ctcgccacct cgatgacctc gggggcaagg
     9061 tcgatcgcgg tcacggcggc accggctttc gcgagcgctt cgctcaacag accgccaccg
     9121 cagccgacat ccgccacccg ggcgccgtgc aaggccaccc gcgctgcgac gaatgccaac
     9181 cgcaccggat tcaggtcgtg caggggacgc gattcgccat ccggatccca ccagcggtgc
     9241 gcgagcttgc cgaagcgggc gagctcttcg ggatcggcgt tgtggctcgc gacgttcatg
     9301 ccgcctgcac ctgctccacg ggccggaggt cccactcgta cacgctgacc tgcgaggaac
     9361 ccgtcacgtg cacgggatcg gcgaaggcca tcccgcgcgc ctcttccagc gattcggcgc
     9421 gcaccaggta tgcccctccg gaccggtccg caaagggacc gaatgcttcc agcgcgcccc
     9481 gcgccttcaa ggcgtcgaga tgcgaatagt gcgcctcgat caccgccgga tcgaaccggg
     9541 gcgtgcgcag ggtgaggacc aggtaacgct tcatggggtt tccgttgtca aagcgcgatg
     9601 atccgctcgc gccaacggcg ggccctttcg agcacgcccg caagatcgaa atcgaccagt
     9661 tcgccattgc cgagcttgcg atggccatcg atccacacgt cgctcacctg gcgccgcgac
     9721 accgcgtagg cgagctgcga gaccacattg tagaggggct gggtttcgac ctcatccatg
     9781 cgcaccgccg cgaggtccgc gcgcttgccc ggctcgacgg agccgatggc ggcctgcaag
     9841 cccatcgcgc aggcgccgcc gagggttgcc gcgcgcaacg cgaaggcgtc atcgaaggcc
     9901 gccgcgtcgc cggcgaccgc cttggcgagc agcgcggccg tccgcagttc gccgaacatg
     9961 tcgaggtcgt tgttggacgc gcaaccgtcg gtgccgagcg caagattcac gccggcgcga
    10021 cgcaactttt ccgcgggaca aaagccggac gcgagcttga ggttcgattc ggcacaatgc
    10081 accaccgata cgccggcttc cgcgcaggcc gcgatttccg catcgctgac ctgggtcatg
    10141 tgcaccgcga ttagccggtc gttgacgagc ccgagtttct gcaagcgctg catcgggcgc
    10201 aggccgtgct cgcgccggga gtcctcgact tcctgcgccg tttccatcag gtgcaggtgc
    10261 accggcacgt ccaactggtc cgacagcatt cggatgcgtt cgaac
//
LOCUS       DASSNH010000177         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1297173, whole genome shotgun sequence.
ACCESSION   DASSNH010000177 DASSNH010000000
VERSION     DASSNH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297173"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFK00_06665"
     CDS             <1..232
                     /locus_tag="VFK00_06665"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET7592666.1"
                     /translation="WGLLALAGLGVSMWKNDRMLAQSSLLVFGATAGKVLLYDLSGSP
                     PLARIVSLVVLGLTFYVAGMLYQKLVATCGAA"
     gene            complement(240..1511)
                     /locus_tag="VFK00_06670"
     CDS             complement(240..1511)
                     /locus_tag="VFK00_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HET7592667.1"
                     /translation="MGIGFAVGLFGGLIVHAATGGEAPWLEWLTHWITVPAGQIFLRL
                     LFMLVLPLLFSALVIGISEMGSMRALGRIGWKTLAYTIVVSTIAVLIGVVLVNVFRPG
                     GNVDPTAANDLLAHASSRAGAIIESSRTQPEGLQMLLGIIPSNVFAAAAGNDILAVMF
                     FAVMVGVGIVLNGKAPAKRFRDVIEGLFEISMTLIDLVIRLAPYAVACLVFNLAALFG
                     WELILTLAGYVGVALLAMALHMFVVYPLAVKVLGGMGPLRFFRGVQEAMVMAFSTASS
                     NATLPTALKVAKEDLKLPPRVSRFVLTIGATANQNGTALFEGVTVLFLAQFFGIELSL
                     LQQVTVMFVAILGGIGTAGVPAGSLPVVALICAMVGVPPEGIGLILGVDRLLDMCRTT
                     LNVTGDLAAAVVVSRGEEDVTVDAALAESKG"
     gene            1743..>2917
                     /locus_tag="VFK00_06675"
     CDS             1743..>2917
                     /locus_tag="VFK00_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET7592668.1"
                     /translation="MKDPKAQDGRVGRREDAGTTRALRLLAATAGLLALAGCGANASL
                     PVRAQTGSNPSLPPPAPETIPTVHIAPATGWTGGAEPDAAAGFEVEAFARGLDHPRWL
                     YVLPNGDVLVAETNTPAESNGLGGVKGWVMKRVMKRAGAGVPSPDRIVLLRDSNGDGV
                     ADVRSVFLDHLHSPFGMVLLDGSLYVADTDALLRFPYRQGETSIAASPEKVTELPAGP
                     INYHWTKNVVAGPDGDVLYVSIGSNSNVGGRGMAVEEGRARIIQVDPRTAAMRTFASG
                     MRNPVGMAVQPGTGALWVVVNGRDELGGDLVPDYLTHVVPGGFYGWPYSYYGQHVDPR
                     VKPQRPDLVARAIVPDYALGSHVAPLGLAFYTGDLFPARYGDGAFVGEHGSWNRRPR"
BASE COUNT          492 a          975 c          992 g          458 t
ORIGIN      
        1 gtggggcctg ctcgcgctcg ccggcctcgg cgtgtcgatg tggaagaacg accggatgct
       61 cgcccagtcg tcgctgctgg tgttcggggc gacggccggc aaggtgctgc tctacgacct
      121 gagcggttcg ccgccgctgg ctcggatcgt gagcctggtg gtgctgggac tgaccttcta
      181 cgtggccggc atgctgtacc agaaactggt ggcgacatgc ggcgccgctt gaggcgccgt
      241 cagcctttgc tttccgccag cgccgcgtcc accgtgacgt cttcttctcc acgcgacacg
      301 accaccgcgg cggccaggtc accggtcacg ttgagggtgg tgcggcacat gtcgagcagc
      361 cggtcgacgc ccaggatcag gccgatgccc tcgggcggca ccccgaccat ggcgcagatc
      421 aacgcgacca cgggcagcga gcctgccggc acgccggcgg tgccgatgcc gccgaggatg
      481 gcgacgaaca tcaccgtcac ctgttgcagc aggctcaatt cgatgccgaa gaactgggcc
      541 aggaacagca cggtgacgcc ttcgaacagc gcggtgccgt tctggttggc ggtcgcgccg
      601 atggtcagca cgaaacgcga cacgcgcggc ggcagtttca ggtcctcctt cgcgactttg
      661 agggcggtcg gcagggtggc gttgctcgac gcggtcgaga acgccatcac catcgcttcc
      721 tgcacgcccc ggaagaaacg cagcggcccc atcccgccca gcaccttcac cgcgagcgga
      781 tacaccacga acatgtgcag cgccatcgcc agcagcgcaa cgccgacata gcccgccagc
      841 gtgaggatca gttcccagcc gaacaatgcg gcgaggttga aaaccaggca ggccacggcg
      901 tagggcgcga gccggatcac taggtcgatc agggtcatcg agatctcgaa caggccttcg
      961 atgacatccc tgaagcgctt cgcgggcgcc ttgccgttga gcacgatgcc gacgcctacc
     1021 atcacggcga agaacatcac ggccaggatg tcgttgccgg ccgcggccgc gaacacgttg
     1081 ctcgggatga tgccgagcag catctgcagg ccttcgggct gcgtgcggct cgattcgatg
     1141 atcgcgccgg cgcgcgagga agcatgcgcc agcaggtcgt tggcggcggt cggatcaaca
     1201 ttgcccccgg gccggaacac gttgacgagc accacgccga tcagcaccgc gatggtcgac
     1261 accacgatcg tgtaggcgag ggtcttccag ccgatccgtc ccagcgcgcg catgctcccc
     1321 atttcggaga tgccgatcac cagtgccgag aacagcaacg gcagcaccag catgaacaga
     1381 aggcgcagga aaatctgccc tgcgggtacc gtgatccagt gggtgagcca ttcgagccac
     1441 ggcgcctcgc cgccggtcgc ggcgtggacg atcagcccgc cgaagaggcc gaccgcgaac
     1501 ccgatcccca ttttggtgtg cagtttcacg gccagactcc tgcgaatgtc gcggcagtgt
     1561 agcccgggca gcgaccgccc catcgcgacc gaagagcggg tcgcggccaa gggcccctcc
     1621 tgcggaagcg ccaagttttt tcgggagcgc gccgtgcacg cggctgtcgc agcggggacg
     1681 atcaatgcgt tcggcggatc gctcaggctc gattcatggc gacgcgccta tgccagcttc
     1741 cgatgaagga tcccaaggcc caggatggac gggtaggacg gcgcgaggac gccggaacga
     1801 cgcgcgcatt gcgactgctt gccgcgacgg cgggtctgct ggcactcgcg ggctgtggcg
     1861 ccaacgcgtc cctgccggtc agggcgcaga cggggtcgaa tccatcgcta ccgccgcccg
     1921 ctcccgaaac gattcccacc gtccacatcg cgccggccac aggttggacg ggcggtgccg
     1981 aacccgatgc ggcggccggc ttcgaggtcg aagcgttcgc acgcgggctc gatcacccgc
     2041 gctggcttta cgtgttgccg aacggcgacg tcctggtggc cgagaccaac accccggccg
     2101 agtctaacgg cctgggcggc gtcaaaggct gggtcatgaa gcgggtgatg aagcgcgcgg
     2161 gcgcgggcgt gccgagcccc gaccgcatcg tgctgctgcg cgacagcaac ggcgacggcg
     2221 tcgcggacgt ccgcagcgtc ttcctcgatc acctgcattc gccgttcgga atggtcttgc
     2281 tggacggctc gctgtatgtc gccgacaccg acgcactgtt gcgctttccg tatcgccagg
     2341 gtgaaacgtc cattgccgcc agcccggaga aggtgacgga actgcccgcc gggccgatca
     2401 actaccactg gaccaagaac gtcgttgcgg ggccggacgg cgatgtcctt tacgtctcga
     2461 tcggttccaa cagcaacgtc ggcggtcgcg gcatggcagt cgaggagggg cgcgcccgga
     2521 tcatccaggt cgatcccaga accgctgcca tgcgcacgtt cgcgtccggc atgcgcaatc
     2581 cggtcggcat ggccgtgcag ccgggaaccg gcgcgctgtg ggtggtcgtc aatgggcgtg
     2641 acgaactggg cggcgacctc gttcccgatt acctgaccca cgtggtgccc ggcggcttct
     2701 acggctggcc gtacagctac tacggacagc acgtggatcc acgggtgaag ccgcagcgtc
     2761 ccgacttggt ggcgcgcgcg atcgttcccg attacgcact cggctcgcac gtcgcgccct
     2821 tggggcttgc gttctacacc ggcgatctgt tcccggcgcg gtatggcgat ggtgcattcg
     2881 tcggggagca cggatcgtgg aaccggcggc cgcgcag
//
LOCUS       DASSNH010000178        14324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_556771, whole genome shotgun sequence.
ACCESSION   DASSNH010000178 DASSNH010000000
VERSION     DASSNH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556771"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFK00_06680"
     CDS             <1..301
                     /locus_tag="VFK00_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7592669.1"
                     /translation="ERLPAHPPAASILAIAYLAVFGSIIAFSAYLFLVKHARPTLTAS
                     YAYVNPPVAVLLGALIAGEHVGMAEIGGMVVILVGVAIITLAHSGRGRRRAATAE"
     gene            complement(438..1745)
                     /locus_tag="VFK00_06685"
     CDS             complement(438..1745)
                     /locus_tag="VFK00_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7592670.1"
                     /translation="MKRFLVNTGMVVLAIVALAIWVKLPWIGALVLAALFALWMALTR
                     SGRQAASVTKVGIGTLRDRLGSSLVIVIGIAGVVAVLVALLAMGEGLQATLNSGGRND
                     TAIVLRGGSVSESQSVLVRDDVDAIEQAAGIAHGASGKPMASAELVVAANLRLRDSQD
                     EAAAQLRGVDESAWALRPSLEMLEGRKFKPGMRELVVGEAAHRQFAGLDVGKDIKLGP
                     EVWKVVGVFQSDGAWNSELWADRQTVASAYRRGNSAESVLVKLTGPAAFATFKAALAA
                     DPRLKVEASTTHDYFAKQSEGLSKIIRVVGLTVGIIMAIGALFGALNCMFAAVASRAR
                     EIATLREIGFRGTPVVVSVMLETMLLALAGGVLGALIVWLIFNGYHASTLSGFTQLMF
                     QFKVGPDLIWTGLKWALAIGFVGGLFPAVRAARLPVTTALREL"
     gene            complement(1755..2918)
                     /locus_tag="VFK00_06690"
     CDS             complement(1755..2918)
                     /locus_tag="VFK00_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7592671.1"
                     /translation="MKYFHLVWSALFRRKTRTILTLVSIIAAFLLFGLLDSVRVAFTA
                     GNNVAGAGRLVVTSRMSMQQALPESLGARIANVPGVSSVAYANWFAGVYQDPKNQVFS
                     FAVSPNYLDLYPELELPADQREAWDRTRTGAIVGAALAKRFGWKIGHKIPLQSTIWPS
                     KSSGSNTWTFDIAGIFHAKDAKDKGNEQMLMFHWKYFDENNRFGSGEVHWYVVKVADP
                     SHADSVASAIDALSANSDHETRAQTEQAFQASFIKQLINIGFVVAAIMGAVFFTLLLL
                     TGNTMMQAVRERTSELAVLKTIGFSGRAVLAMVLSEGVLMLLFGGLIGMGLVALIVPG
                     MAAASGGMVPIHGVAGQTWALGIGLMIVIGLVVGLVPAWRAMRLNIVDALSGR"
     gene            complement(2933..3670)
                     /locus_tag="VFK00_06695"
     CDS             complement(2933..3670)
                     /locus_tag="VFK00_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7592672.1"
                     /translation="MNTAVEEARGAETGSNASQPLVQIRGLSKTYTRGKQKLEVLHHI
                     DLDIARGDFFALMGPSGSGKTTLLNLIGGLDSPTEGSLTVDGKRIDKMGESQLARWRA
                     SNVGFVFQFYNLMPALSAQKNVELPLLLTRLSGARRKKNAAIALQLVGLADRATHKPS
                     ELSGGQQQRVAIARAIVSDPSLLVCDEPTGDLDRQSAEDVLTLLQRLNREHGKTIVMV
                     THDPKAAEYASHTLHLDKGTLAEQHTP"
     gene            complement(3888..5126)
                     /locus_tag="VFK00_06700"
     CDS             complement(3888..5126)
                     /locus_tag="VFK00_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7592673.1"
                     /translation="MSDGDSKDLLHELRIDSAHRDDDSHGPRWPWIVGILVMVLIAAG
                     AAAFVLRPRAVTVETASAVALSAAGSDAAVLQATGYVVARRQATVSAQITGTLIEVNV
                     EEGEHVTAGQVLARLDDTQFVDQLNAAKAQYAAAQAQVTQAQASLKQASQDAERTNAV
                     VEKGYVSRQSAQQANTQVAIGRAALNVAVKQAEAAAANVKLAQVNEDYTIVRAPFDGV
                     VTDKAAQIGEIVSPLSAGGGFTRTGVATIVDMNSLEVDVDVNESYISRVKPGMPVEAI
                     LDAYPDWKVPAHVIAIIPSADRSKATVKVRIALDKKDPRVLPEMGVRVSFLEKSGNNA
                     KPLPGVLVPKSAVVQRGGKDVVFVVAGNSAKETPVTTGGDFSDMKQILAGVSAGSEVV
                     KAPPAGLKDGEAVKVKAGNN"
     gene            5314..6003
                     /locus_tag="VFK00_06705"
     CDS             5314..6003
                     /locus_tag="VFK00_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7592674.1"
                     /translation="MHYLFEPFPAPGLQIHGSDARFPVHRIYCIGRNYAEHAKEMGAQ
                     AVGRANPVFFMKPADAIVAGGGDIPYPSATRELHHEVEMVVALARGGRDIDAEAALDC
                     VFGYGVGLDLTRRDLQAAMKAKGLPWDVAKGFDASAPVSALRAASECGHPTHARLSLS
                     VNGAVRQDTDIADMIFAVPEIIAELSKLFELKAGDLVFTGTPAGVGPLQRGDAFRAAL
                     QDIAMLEGRIV"
     gene            6286..6687
                     /gene="mscL"
                     /locus_tag="VFK00_06710"
     CDS             6286..6687
                     /gene="mscL"
                     /locus_tag="VFK00_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET7592675.1"
                     /translation="MSFAKDFKAFIMRGNVVDLAVAVVIGGAFGAVVKSLVSDIIMPP
                     LGLITGGIDFSRMKVVIQAATATRKEVAITYGAFINTIITFLIISLAIFVVIKALQKV
                     HPRKTDEAPPAPPADVVLLTEIRDLLKQQQH"
     gene            6754..7311
                     /locus_tag="VFK00_06715"
     CDS             6754..7311
                     /locus_tag="VFK00_06715"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HET7592676.1"
                     /translation="MPMEILQADITSLHVAAIVNAANESLLGGGGVDGAIHRAAGPKL
                     LDACRAVPEVRPGVRCPTGEARITAGFDLPARFVIHTVGPVWRGGGHGEPELLAACHR
                     NTLALAREHHIDSLAFPAISCGVYGYPRDAAAGVAMAVLGEEQHRTRTPHRIVLCAFD
                     ARMAKAWRQAAERLHVEVVDSPGSH"
     gene            complement(7383..9230)
                     /locus_tag="VFK00_06720"
     CDS             complement(7383..9230)
                     /locus_tag="VFK00_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HET7592677.1"
                     /translation="MSAARCFVLLALLALLALFAPLAQAAGASGGSAGDVHAFLDRSH
                     ATLGDTVTLNIRSTGALDQPDLSPLRNDFEVLGTSRSTSVQIVNGQATSTSQLGIALK
                     PLRAGTLTIPALAVGGGHTQALTLQVGAAPSGGTGKVGDPVFMETSVLSSSPWVGQET
                     VYTVRLFYLPGVDGALGDPSADGARLVQLDRDHRYVTQRNGYTYKVIERSWALIPQRS
                     GNIRVHGPVFQGQRTGPGIPNAWFNNPNGLLNVPLHGFGGAVRASAPVAQIDARAPPA
                     NAGKPWLPAQSVELKLSGVPAGGQIDAGAPLTVTLAISASGQPADALPEPQLPAIAGA
                     RVYPDQTRDATNDSGKWLQGTRTRTFAIVPERNGTLTIPAITLDWWNVEQNRPEQART
                     TARTLEVSGVVAGSSAPAAPPAASPAPSAAPATAAPADAGAAASTSSGSRWRDIALAS
                     LALWILLALAGFAWWLSLRRRRTAALATGEMTAAAPAPKAVPRPSSTGPRSRAAKQGT
                     RMDAKALQREALDAARAGDAAACEHGLLAWARASGHGVASVGALRDALADPAQRAALD
                     ALQRARWQGVDVADACSQVSTAFAKGFAWRSGGEVNGRDGALPPLYPLP"
     gene            complement(9227..11083)
                     /locus_tag="VFK00_06725"
     CDS             complement(9227..11083)
                     /locus_tag="VFK00_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7592678.1"
                     /translation="MNAFGFMPDFLGLHFLRPWWCLALLPLPLVLWALARGGSGRAML
                     SRIADAALLPYLVHDGGARRRLALALAALAWLLAVAALSGPAWRKLPAPLYVNGAARV
                     VALSLSNDMLAQDLKPDRITRAKFAVRDLLDEAGDARTALVAYAGAAFTVAPLTDDKR
                     TVLNLLHALRPDVMPVPGNDAAAGIRQASRLLQQAHVEGGEIVLVTDGADAAAVDAAS
                     AARGKGIRVDVLGIGTTAGAPVPRSGGGFASGSSGLLMAQRDDSALAAVARAGGGRYA
                     VLQSDGGGTASFGAPVAESGHASAAQRAQIWRDGGIWLLPVLLVLAALAFRRGWLLVL
                     ALAAMPVAMPTAHASTWSSLWANSDQRALHALQHGDAPQAQKLAGTPGIRGAADYRQG
                     DYAEAARAFAQGDDARASYNLGNALAKQGAYTKAIGAYKQALRQDPGFEDARANLDAV
                     EAWLKKHPPAASQQQDGHPQPGNDQSGSPSSQSGSGQRQPSTSSGSQQAGQGAQSNPS
                     ATGQDRDGRGGPGQDGGASATEAERQRRQADEAARGLARELQGKHDDRRKPGSGKGAF
                     ALGQAEPRDDSGLDASQRAILQSVPDDPGALLRRKFRLEWEQRNGSQQENGQ"
     gene            complement(11080..12057)
                     /locus_tag="VFK00_06730"
     CDS             complement(11080..12057)
                     /locus_tag="VFK00_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7592679.1"
                     /translation="MTFAWPWMFMLLPLPWLAWKLLPPAAPGAALRLPQRVALADAAS
                     GLRASRWRVGVACAAWLLLVGAAARPQQPAPPQAMHHTGRALMLAVDCSGSMAIEDMR
                     FSDRIVSRFAAVKAIAARFIEQRSGDDVGLVLFGSHAYLLTPMTFDVATVAKQMDGAA
                     IGLAGRETAIGDAIVLAVKHLSALPARARVLVLLTDGVNTAGNVLPLEAAKLAKAAGV
                     RIYTIGIGSNAQELGFFGMQLPASNELDLQVLTKISEETGGRFFRAADAGELAAAYHT
                     IDALEPLAEGQSLLRPNHEWYPWPLGLALLLGCMLLPWRSLVPRRTVRA"
     gene            complement(12054..12590)
                     /locus_tag="VFK00_06735"
     CDS             complement(12054..12590)
                     /locus_tag="VFK00_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HET7592680.1"
                     /translation="MNDSGPVLRDIHLPPAAWWPLAPGWWIVVGLVLLATAVVAWWSW
                     RRAGGGVRRAALREIDALAAAYARNGNDVMFADGASRLLRRVARVVEPGAASLAGEPW
                     RAFLHQYARDAATQASLDRLLEARYLPRPALDVPAVLAALRAWCGNALAHGRRHAPDH
                     TAAPRITTTGAPGEGTAS"
     gene            complement(12587..13585)
                     /locus_tag="VFK00_06740"
     CDS             complement(12587..13585)
                     /locus_tag="VFK00_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7592681.1"
                     /translation="MPRREQVGHGKFIESMGATITQASDHDLGITRVDLAELLAMRAR
                     AAKPVAQRARSPLTGGHASALRGRGMDYAESRIYEPGDDARNIDWRRTARSGKWLTKL
                     FEAEREHGLLLLVDTHATMRFGTRRRYKSVAAARAAAWIAWTCVRASDRIGAMAFGAV
                     RSAVDPHAGTRGALAVLGALARWDESAQQDAGSAEPLSAALARAQRLVTPGSRAWLLS
                     DGWCTDDGAVQALRRLARHADLRVAIVVDALEREPARAGTCVFETASGKPGRGRFVVD
                     LGGAESRAQFGDRMSQGRRRLADACDKATVPWVLFATTDEPDIVVAPAWRRRTRRS"
     gene            complement(13736..>14324)
                     /locus_tag="VFK00_06745"
     CDS             complement(13736..>14324)
                     /locus_tag="VFK00_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7592682.1"
                     /translation="TIGRRSWPLPRLFLVMATQNPIEQEGTFPLPEAQLDRFLLHVPV
                     RYPESGSERAIMRLARGRAREELGRHQPPDRLLGQSDIFAAREQVLDLHMAEGLEEYI
                     VQLVLATRDPRPWAPKLREKIAWGASPRASIALERCARAAAWLAGRDYVLPEDIQSIA
                     PDALRHRVLLGYEAEAEGLHVDDVISQLLERVPLP"
BASE COUNT         2208 a         5079 c         4784 g         2253 t
ORIGIN      
        1 cgaacgcctg cccgcgcatc cgcccgccgc ttcgatcctg gcgatcgcgt acctggccgt
       61 gttcggctcg atcatcgcct tttccgcgta cctgttcctg gtcaagcacg cgcggccgac
      121 gctgaccgcg agctacgcct atgtcaaccc gccggtcgcg gtgctgctgg gtgcgctgat
      181 cgctggcgag cacgtcggca tggccgaaat cggcggcatg gtcgtgatcc tcgtcggtgt
      241 cgcgatcatc acgctggcgc actcggggcg cggaaggcgg cgtgcagcca cggcagagta
      301 ggctccgcag tcccgggagc aatgcgctat cacgccggcc agtctcgccg aaccaattcc
      361 acaagctcaa ggatgctcgc tcggcggtat gagggccgcg aattttgcgg gcccgcgagt
      421 acgctgcgcg cgagggctta caactcacgc agcgccgtcg tgaccggcaa cctcgccgcg
      481 cgcacggcgg gaaacaggcc gcccacgaac ccgattgcaa gcgcccactt caggcccgtc
      541 cagatcaggt cgggaccgac cttgaactgg aacatcaact gggtgaaacc gctcagggtc
      601 gaggcgtggt agccgttgaa gatcagccac acgatcagcg caccgagcac gccgcccgcc
      661 agcgcgagca gcatcgtctc cagcatcacc gacaccacca ccggcgttcc ccggaagccg
      721 atctcgcgca gggtggcgat ttcgcgcgcg cgcgatgcaa ccgccgcgaa catgcagttg
      781 agcgcgccga acaaggcgcc gatcgccatg atgatgccga ccgtcaggcc caccacccgg
      841 atgatcttgc tcaagccttc agactgcttc gcgaaatagt cgtgggtggt gctggcctcg
      901 accttcaggc gcggatccgc ggccagggcg gccttgaagg tggcgaacgc cgcggggccg
      961 gtcagtttca ccagtaccga ttcggcgctg ttgccccgcc ggtacgccga ggccaccgtc
     1021 tggcggtcgg cccacaattc cgaattccag gcgccgtcgc tctggaacac gccgaccacc
     1081 ttccacacct cgggcccgag cttgatgtcc ttgccgacgt cgagcccggc gaactgcctg
     1141 tgcgcagctt cgcccaccac cagttcgcgc atgccgggct tgaacttgcg gccttcgagc
     1201 atctcgaggc tcggccgcaa agcccaggcg gactcgtcca cgccgcgcag ttgcgcggcg
     1261 gcttcgtcct gcgaatcgcg caggcgcagg ttggccgcca ccaccagttc cgccgaagcc
     1321 atcggcttgc ccgaggcacc gtgcgcgatg ccggcggctt gttcgatggc gtccacgtcg
     1381 tcgcgcacca gcaccgattg cgactcggat accgatccac cacgcagcac gatcgccgtg
     1441 tcgttgcgcc cgccgctgtt tagcgtggct tgcaggccct cgcccatcgc cagcagcgcg
     1501 accagcacgg cgaccacgcc ggcgatgccg atcacgatca cgagcgaaga accgagccgg
     1561 tcccgcaagg tgccgatgcc gaccttggtc accgaagcag cctgcctgcc gctgcgcgtc
     1621 agtgccatcc acagcgcgaa cagggcggcg aggaccagcg cgccgatcca tggcagcttc
     1681 acccagatcg ccagcgcgac gatcgccagc accaccattc cggtattgac caggaatctt
     1741 ttcatggctg ttcctcagcg tccgctcagc gcgtcgacga tgttgaggcg catcgcgcgc
     1801 cacgccggca cgaggcccac gaccaggccg atcacgatca tcaggccgat cccgagcgcc
     1861 cacgtctgtc ccgcgacgcc gtggatcggc accatgccgc cgctggcggc ggccatgccg
     1921 ggcacgatca gcgccaccag gcccatgccg atcaggccgc cgaacagcag catcagcacg
     1981 ccttcgctca gcaccatcgc cagcacggcg cgtccggaaa aaccgatcgt cttcagcacc
     2041 gcgagttccg aggtgcgctc gcgcaccgcc tgcatcatgg tgttgccggt caacagcagc
     2101 agcgtgaaga acaccgcgcc catgatcgcc gcgaccacga agccgatgtt gatcaactgc
     2161 ttgatgaagg acgcctggaa ggcctgctcg gtttgtgcgc gcgtttcatg gtcggagttg
     2221 gcggacagcg catcgatcgc actggccacc gagtccgcgt gggatggatc ggccaccttc
     2281 accacgtacc agtgcacttc gccggaaccg aaacggttgt tctcgtcgaa gtacttccag
     2341 tggaacatca gcatctgctc attgcccttg tccttcgcgt ccttggcgtg gaagatgccg
     2401 gcgatgtcga aggtccaggt attgctgcca ctggatttgg acggccaaat cgtcgactgc
     2461 agcggaatct tgtggccgat cttccagccg aagcgcttgg ccagcgcggc gcccacgatt
     2521 gcgccggtgc gggtgcggtc ccacgcctcg cgctggtcgg ccggcagctc gagttccggg
     2581 tacaggtcca ggtaattcgg gctgaccgcg aaactgaaga cctggttctt cgggtcctgg
     2641 tagacgcccg cgaaccaatt ggcgtacgcc accgagctga cacccgggac gttggcgatg
     2701 cgggcgccga ggctctccgg cagtgcctgc tgcatcgaca tccttgaagt cacgacgagg
     2761 cggccggcgc cggccacgtt gttgcccgcg gtaaacgcca cccgcacgga atcgagcagg
     2821 ccgaacagca ggaacgccgc gatgatcgac accaacgtca ggatcgtgcg cgtcttgcgc
     2881 cggaacaggg cactccagac cagatggaaa tatttcatgg cgtggctccc gctcagggcg
     2941 tgtgttgctc ggccagcgtg cctttgtcga ggtgcagggt gtggctggcg tactcggcgg
     3001 ccttgggatc gtgggtgacc atcacgatgg tcttgccgtg ctcacggttg agccgttgca
     3061 gcaacgtcaa tacgtcctcg gcggactggc ggtcgagatc gccggtcggc tcgtcgcaca
     3121 ccagcagcga cgggtcggaa acgatcgccc gcgcgatcgc gacgcgctgc tgctggccgc
     3181 cggagagttc gctcggcttg tgcgttgcgc ggtcggccag ccccaccagt tgcagtgcga
     3241 tcgccgcgtt cttcttgcgc ctggcgcccg acagcctggt cagcagcagc ggcagctcga
     3301 cgttcttctg cgcgctcaat gccggcatca ggttgtagaa ctggaacacg aaaccgacgt
     3361 tggacgcgcg ccagcgggcg agctggcttt cgcccatctt gtcgatgcgc ttgccgtcca
     3421 cggtcaggct gccctcggtc ggcgaatcaa gtccgccgat caggttcagg agcgtggtct
     3481 tgccggaacc cgacggcccc atcagggcga aaaagtcgcc gcgcgcgatg tcgaggtcga
     3541 tgtggtgcag cacctcgagc ttctgcttgc cgcgggtgta ggtcttggaa aggccgcgga
     3601 tctggaccag cggctggctg gcattgctgc ccgtctcggc gccgcgggct tcttcgactg
     3661 ctgtgttcat cgttgtctcc tggcatttga accccttccc cgccggggaa agggggcagt
     3721 gatgaggcac gaggctggcg caacacccaa tcctgcgcga gtccgaaccc tcatccggcc
     3781 cttactcgcg ccgtccatgg cgctcgccct tcgggccgac taacgtcgtt cacggccgat
     3841 ccagtcggcc gtagtcgggc caccttctcc cggagggaga aggaaaatca gttattgccc
     3901 gccttcacct tcaccgcttc gccatccttc aggcccgcgg gcggcgcctt caccacttcg
     3961 gagcccgcgc tgacgccggc gaggatctgt ttcatgtcgg agaagtcgcc gccggtcgtc
     4021 acgggcgttt ccttcgcgct gtttcccgcc accacgaaca ccacgtcctt cccgccgcgc
     4081 tgcaccaccg cggatttcgg taccagcacg ccgggcaacg gcttggcgtt gttgccggat
     4141 ttttcgagga atgaaacgcg cacccccatc tccggcagga cgcgggggtc cttcttgtcc
     4201 agcgcgatgc gcaccttcac ggtcgccttg ctgcgatcgg cgctggggat gatcgcgatc
     4261 acgtgggccg gaaccttcca gtcgggatag gcgtccagga tcgcctcgac cggcatgccc
     4321 ggcttcacgc ggctgatgta ggactcgttg acgtcgacgt ccacttccag cgagttcatg
     4381 tccacgatcg tggcgacgcc ggtgcgggta aaaccaccgc ctgccgacag cggcgacacg
     4441 atctcgccga tctgcgcggc cttgtcggtc acgaccccgt cgaacggcgc gcgcacgatg
     4501 gtgtagtcct cgttcacctg cgccagcttg acgttggccg cggcggcttc cgcctgcttg
     4561 accgcgacgt tcaatgcagc gcgcccgatg gcgacctggg tgttggcctg ctgggcgctc
     4621 tgccgcgaga cgtagccctt ttcgaccacc gcattcgtgc gctcggcatc ctggctcgcc
     4681 tgcttcagcg aggcctgcgc ctgcgtgacc tgcgcctgtg ccgcggcgta ttgcgccttc
     4741 gctgcgttca actggtcgac gaactgggtg tcgtccagcc gcgcgagcac ctgccctgcc
     4801 gtgacgtgtt caccctcttc gacgttcacc tcgatcaagg tgccggtaat ttgggcggat
     4861 accgtcgcct gcctgcgcgc caccacgtag ccggtcgcct gcagcaccgc ggcgtcgctg
     4921 ccggccgcgg acagcgccac cgcgctcgcc gtctcgaccg tcaccgcccg ggggcgcagc
     4981 acgaacgccg cggcacccgc ggcaatcagc accatcacca agatgcccac gatccatggc
     5041 cagcgcggac cgtgggagtc gtcgtcgcgg tgggcgctgt cgatgcgcag ttcgtggaga
     5101 aggtccttgg aatcgccgtc gctcacttgg gtccccgatg cgcgcggtgc gttgtggcgg
     5161 cgcaagtgtg ccatagtttc cccgctggat tgccgcggcg tcacccgcag gcatcaagca
     5221 tcacggctga cagctgtcac ccctcccaag acccggcaga acgaggcgcg gcgaccctac
     5281 aatgcgcgca tcgcctgcac ggaatcgcgc cgcatgcact acctgttcga accgttcccg
     5341 gccccgggct tgcagatcca cggcagcgat gcacgttttc ccgtgcaccg tatctattgc
     5401 atcggccgca actacgccga gcacgcgaag gagatgggcg cgcaggccgt gggccgcgcc
     5461 aatccggtgt tcttcatgaa gcctgccgac gcgatcgtgg ccggtggcgg cgacatcccg
     5521 tacccatccg cgacgcggga attgcaccac gaagtcgaaa tggtggtggc gctggcgcgc
     5581 ggcgggcggg acatcgatgc ggaggccgca ctcgattgcg tgttcggcta cggcgtcggc
     5641 ctcgacctga cccgccggga cctgcaggcg gcgatgaagg ccaaggggtt gccgtgggac
     5701 gtcgccaagg gcttcgatgc ctcggcgccg gtctcggcgt tgcgggcggc gagcgagtgc
     5761 ggccatccca cgcacgcgcg gctgtcgctc tcggtcaacg gcgcggttcg ccaggatacc
     5821 gacatcgccg acatgatctt cgcggtgccg gaaatcatcg ccgaactgtc gaaactgttc
     5881 gagctgaagg ccggcgacct ggtcttcacc ggcacgccgg cgggcgtggg cccgctgcaa
     5941 cgcggcgacg cgttccgcgc ggcgttgcag gacatcgcga tgctggaagg acgcatcgtc
     6001 tgaccgcgcg gtcctgatgc aacgcagtgg aatccgggaa tccatggccg acccccggat
     6061 tgattcgcga catcgatgtc gttcgcgctt cggcaaggcg gtcatccccg gcactcgacc
     6121 ttcgggcggc tttttcgtac gcctgtgcat tccgcagtga ttccacgcct tgcggtcggc
     6181 gcgctgtact cgtgaaaagt tgcgcggggc cggggatagt gactaaatgg ggccgccgtg
     6241 acgcggccgg ggggaagctt gcgcccacct cagaaggagt acgcaatgag tttcgcgaag
     6301 gacttcaagg cgttcatcat gcgcggcaac gtggtcgacc tcgccgttgc ggtggtcatc
     6361 ggcggcgcct tcggtgcggt cgtcaagtcg ctggtcagcg acatcatcat gccgcccctg
     6421 ggcctgatca ccggcggcat cgatttctcc aggatgaagg tggtcatcca ggcggctacc
     6481 gccaccagaa aggaagtcgc gatcacctac ggcgcattca tcaataccat catcacgttc
     6541 ctcatcattt cgctggcgat cttcgtcgtc atcaaggcgt tgcagaaggt gcatcccagg
     6601 aagaccgatg aggcaccacc tgctccgccg gcggacgtgg tattgctgac cgagatccgc
     6661 gacctgctga aacagcagca gcactgatca ccgggaattg cgtcacctgg ctcgcaccga
     6721 ccacgggctc gacgatcctg cgacccgcca gccatgccca tggaaatcct ccaggcggac
     6781 atcacctcct tgcacgtcgc ggcgatcgtc aatgcagcga acgagtcgct gttgggcggc
     6841 ggcggggtgg acggtgcgat ccaccgcgcc gcggggccga aactgctcga cgcttgccgc
     6901 gcggttcccg aggtacggcc cggtgtgcgc tgcccgaccg gcgaggcccg catcacggcg
     6961 ggcttcgacc tgcccgcgcg attcgtgatc cacaccgtcg gaccggtgtg gcgcggcggc
     7021 gggcacggtg aacctgaatt gctggcggcc tgccatcgca acacgttggc gctggcacgg
     7081 gagcatcaca tcgacagcct ggcattcccg gcgatcagtt gcggcgtcta cggctatccg
     7141 cgcgatgccg ccgcgggggt ggcgatggcg gtgctgggcg aagaacagca ccggacccgc
     7201 acgccgcacc ggatcgtgtt gtgcgcgttc gacgcgcgca tggcgaaggc ctggcgccag
     7261 gcggccgaac ggttgcacgt cgaggtcgtg gactcgccgg gctcgcattg accgtttccg
     7321 cggacaatgc aggagggcgc catgcgcgcg atctgacaac cggtcgcggc catgggccgc
     7381 tcctacggca acggatacag cggcggcaat gcaccatcgc ggccgttcac ctcgccaccg
     7441 ctgcgccagg cgaagccctt cgcgaacgcc gtggacactt ggctgcatgc atccgccacg
     7501 tccacgccct gccaacgcgc gcgctgcagt gcatcgaggg ccgcgcgttg cgcggggtcg
     7561 gcgagcgcgt cgcgcaaggc gccgacgctg gcaacgccgt gccctgatgc gcgcgcccaa
     7621 gcgagcaacc cgtgttcaca ggccgcggca tcgcccgcgc gcgccgcatc cagggcctcg
     7681 cgctgcagcg ccttggcgtc catccgcgta ccctgcttcg ccgcccgcga tcgtggaccc
     7741 gtcgaagacg gccgcgggac cgccttcggc gccggagcgg cagccgtcat ttcgccggtt
     7801 gccaaggccg ccgtcctgcg tcgccgcaac gacagccacc acgcaaaacc ggccagcgcc
     7861 agcaagatcc aaagcgcaag gcttgccagc gcgatgtcgc gccaccggct gcccgacgac
     7921 gtggatgcgg ccgcacctgc atccgcaggg gcagccgttg ccggcgcggc cgacggagcc
     7981 ggcgacgccg cgggtggcgc ggccggagcc gaggagccgg ccaccacgcc gctgacctcc
     8041 agggtgcgcg ccgtggtcct cgcctgctcc gggcggtttt gttccacgtt ccaccagtcc
     8101 agggtgatcg cggggatcgt gagcgtgccg ttgcgctccg gcacgatcgc gaaggttcgg
     8161 gtgcgcgtcc cctgcagcca tttgccactg tcgttggtgg cgtcacgggt ctggtcggga
     8221 tagacgcgcg cgccggcgat cgcgggcaac tgcggttccg gcagcgcatc cgcgggctgc
     8281 ccgctggcgc tgatggcgag cgtcaccgtc aacggtgctc ctgcatcgat ttggccaccg
     8341 gcaggcacgc cggacaactt cagctcgacg ctctgggccg gcaaccacgg cttgccggcg
     8401 ttggccggcg gcgcgcgcgc gtcgatctgc gcgacgggcg ccgaggcgcg cactgcccct
     8461 ccgaagccgt gcagcggcac gttcaacagt ccgttgggat tgttgaacca ggcattggga
     8521 atgccggggc cggtgcgctg gccctggaac accggaccgt ggacgcggat gttcccgctg
     8581 cgttgcggga tcagtgccca gcttcgttcg atgaccttgt aggtgtagcc gttgcgctgg
     8641 gtgacatagc ggtgatcgcg gtcgagctgg accaggcgcg cgccgtcggc gctcgggtcg
     8701 cccaaggcgc catcgacacc cggcaggtag aacaggcgca cggtgtaaac ggtctcctgc
     8761 cccacccacg gcgaactcga cagcacgctc gtttccatga acacgggatc gcccaccttg
     8821 cccgtgcctc cgctcggcgc tgcgccgacc tgcaaggtca gggcctgggt gtggccgccg
     8881 cccaccgcga gggcgggaat cgtcaaggtg cccgcgcgca gcggtttgag cgcaatcccg
     8941 agttgcgacg tgctggtcgc ctggccattg acgatctgca cgctggtgga acgggaggtg
     9001 ccgagcactt cgaaatcatt ccgcaacggc gacagatccg gttggtcgag cgcgccggtc
     9061 gagcggatgt tgagcgtgac cgtatcgccg agcgttgcat ggctgcgatc gaggaacgcg
     9121 tgcacgtctc ccgcggaacc gcccgaagca cccgccgcct gcgcgagcgg cgcgaacagc
     9181 gccagcagcg ccagcagcgc cagcagcacg aaacaccgtg cagcgctcat tgcccgttct
     9241 cctgctgact gccgttgcgc tgctcccatt cgaggcggaa cttgcggcgg agcaatgcac
     9301 ccgggtcatc cggcaccgac tgcaggatcg cgcgctggct ggcatcgagg ccggaatcgt
     9361 cgcgcggttc ggcctggccc agcgcaaagg cacccttgcc ggagccgggc ttccgcctgt
     9421 cgtcgtgctt gccctgcagt tcacgggcga gcccgcgtgc cgcctcatcg gcctggcggc
     9481 gttgccgttc ggcttccgtc gccgacgccc cgccatcctg gccgggtccg ccgcggccgt
     9541 cgcggtcctg cccggtcgcg gacggattcg actgcgcgcc ttgcccggcc tgctgcgatc
     9601 ccgatgaggt cgatggctgg cgctgccccg atccggattg cgatgatggg cttccggatt
     9661 gatcgttgcc gggctgcggg tggccgtcct gctgctgcga ggccgcaggc ggatgctttt
     9721 tcagccacgc ctcgacggca tccaggttgg cgcgggcgtc ctcgaagccg ggatcctgcc
     9781 gcaaggcttg cttgtaggcc ccgattgcct tggtgtacgc accctgtttg gcgagcgcat
     9841 tgccgaggtt gtaggacgcg cgcgcgtcgt cgccttgggc gaacgcgcgc gccgcttccg
     9901 cgtaatcgcc ttggcggtag tcggcggcgc cgcggatgcc gggagtccct gccagcttct
     9961 gcgcctgtgg ggcgtcaccg tgttgcagcg cgtgcagcgc gcgctgatcg gagttcgccc
    10021 aaagcgatga ccaggtcgag gcgtgcgccg tgggcatggc gaccggcatc gcggccaacg
    10081 ccagcaccag cagccagccg cgccggaacg ccagcgccgc gaggaccagc agcaccggca
    10141 gcagccagat gccgccgtcg cgccagattt gtgcgcgctg tgcggcgctc gcgtggccgg
    10201 actccgccac cggcgcgccg aacgaggcgg taccgccgcc gtcggactgc agcaccgcgt
    10261 agcggccgcc gcccgcgcgc gccaccgcgg ccagcgccga atcgtcgcgc tgcgccatca
    10321 gcagcccact cgacccgctc gcgaatccgc cgccgctgcg cggcaccggc gcgccggcgg
    10381 tggtgccgat gccaaggacg tccacgcgga tgcccttgcc gcgcgcggcg cttgccgcat
    10441 cgacggcggc cgcgtccgcg ccgtcggtca ccagcacgat ctcgccgccc tccacgtgcg
    10501 cctgctgcag caagcggctc gcctgccgaa tgccggcagc ggcatcgttg ccgggtaccg
    10561 gcatcacgtc gggccgcaag gcgtgcagca ggttcaacac ggtgcgcttg tcgtcggtca
    10621 acggggcgac cgtgaaggcc gcgccggcat aggcaaccag ggccgtgcgc gcgtcgccgg
    10681 cctcgtccag caggtcgcgg acggcgaact tcgcacgcgt gatccggtcc ggtttcaggt
    10741 cctgcgccag catgtcgttc gacaacgaca aagccaccac ccgcgcggcg ccgttgacat
    10801 acagcggcgc cggcagtttc cgccaggccg ggcccgacaa tgccgccact gcaagaagcc
    10861 atgccagcgc cgcgagggcc agcgcgagcc gcctgcgcgc gccgccgtcg tggaccaggt
    10921 acggcaacaa ggcggcatcg gcgatccgcg acagcatcgc ccggccacta cctccgcgtg
    10981 ccagtgccca cagcaccaac ggcaatggca gcagcgccag acaccaccac ggccgcaaaa
    11041 agtgcagccc aagaaaatcg ggcatgaacc cgaaggcgtt catgcgcgca ccgtccggcg
    11101 cggcaccagt gaccgccacg gcagcagcat gcaacccagc agcagcgcga gtcccagtgg
    11161 ccacggatac cactcgtggt tgggacgcag cagtgactgg ccctcggcga gcggttcgag
    11221 cgcatcgatg gtgtggtagg cggcggcaag ctcgccggca tcggcggcgc ggaagaagcg
    11281 gccaccggtt tcttcggaga tcttcgtcag cacctgcaag tcgagctcgt tggacgccgg
    11341 aagttgcatc ccgaagaacc cgagttcctg cgcattggag ccgatgccga tggtatagat
    11401 gcgcaccccc gccgccttgg ccagtttcgc cgcctccagc ggcaacacgt tgcccgcggt
    11461 attgacgccg tcggtgagca acaccagcac gcgcgcccgc gccggcaacg cggagaggtg
    11521 cttgacggcc agcacgatcg cgtcgccgat ggcggtttcc cggccagcga gcccgatcgc
    11581 ggcgccatcc atctgcttgg ccacggtggc cacgtcgaag gtcatcggcg tcagcaggta
    11641 ggcgtgggag ccgaacagga ccaggccgac gtcgtcaccc gagcgctgct cgatgaagcg
    11701 tgcggcgatc gccttcacgg ccgcgaaacg gctgacgatc cggtcggaga agcgcatgtc
    11761 ctcgatcgcc atgctgcccg aacaatccac ggccagcatc agcgcgcggc cagtatggtg
    11821 catggcctgc ggtggcgctg gctgctgcgg tcgcgccgcc gcacccacca acagcagcca
    11881 tgcggcgcat gcgaccccga cgcgccagcg cgaggcacgc agacccgatg ctgcatcggc
    11941 gagcgccacc cgttgcggca ggcgcagcgc ggcaccgggc gcggccggcg gcagcagctt
    12001 ccaggccagc cacggcaatg gcagcagcat gaacatccac ggccacgcga aggtcacgat
    12061 gccgttccct cgccgggcgc gccggtggtc gtgatgcgcg gcgcagcggt gtgatccggt
    12121 gcgtgcctgc ggccgtgggc aagcgcgttg ccacaccacg cgcgcagggc cgcgagcacc
    12181 gcggggacgt ccagggccgg ccgcgggagg tagcgcgctt cgagcaggcg atcgagcgag
    12241 gcttgcgtcg cggcgtcgcg ggcgtattgg tgcaggaacg cgcgccaagg ctctccggcc
    12301 aggctcgcgg cgccgggctc gaccacgcgt gccacgcgcc gcagcagtcg cgacgccccg
    12361 tccgcgaaca tcacgtcatt gccattccgg gcgtaagccg ccgccagcgc atcgatctcg
    12421 cgcaacgccg cgcgccgtac gcctccaccc gcgcgccgcc atgaccacca cgcgaccaca
    12481 gccgtcgcca gcaggacgag tcccacgacg atccaccagc cgggcgccaa gggccaccac
    12541 gccgccggag gcaggtggat gtcgcgcaac acggggccgc tgtcgttcac gagcgcctcg
    12601 tccgccttcg ccacgccggc gccaccacga tgtcgggttc gtcggtggtc gcgaacagca
    12661 cccacggcac ggtcgccttg tcgcaggcgt ccgcgagccg gcgccggccc tgcgacatgc
    12721 gatcgccgaa ttgcgcgcgc gactcggcgc caccgagatc gacgacaaat cttccgcgac
    12781 ccggttttcc cgacgccgtc tcgaacacac aggtcccggc gcgcgccggc tcccgttcca
    12841 gcgcgtcgac cacgatcgcg acgcgcaggt ccgcgtgtcg tgcgaggcgc ctcagtgcct
    12901 ggactgcgcc atcgtcggtg caccatccat ccgacaacag ccacgccctg ctgcccggcg
    12961 tgaccagacg ctgcgcccgc gcgagcgcgg ccgagagcgg ctcggccgag ccagcatcct
    13021 gctgcgccga ctcatcccag cgcgcgagcg cccccagcac cgccagggcg ccgcgcgtcc
    13081 ccgcatgcgg atcgaccgcg gaacgcacgg caccgaaggc catggcgccg atccggtcgc
    13141 tggcacggac gcaggtccag gcgatccacg ccgccgcccg cgccgcggcc accgatttgt
    13201 agcgcctgcg cgttccgaaa cgcatggtcg cgtgggtatc gaccagcaag agcagcccgt
    13261 gctcgcgctc ggcctcgaac agtttggtga gccacttgcc gctgcgcgcg gtgcgacgcc
    13321 agtcgatgtt gcgggcgtcg tcgccgggtt cgtatatgcg ggactcggcg tagtccatgc
    13381 cgcggccgcg cagcgccgat gcgtggccgc cggtcagtgg cgaccgcgcg cgctgcgcga
    13441 cgggttttgc cgcgcgcgca cgcatcgcga gcagctccgc gagatcgacg cgggtgatgc
    13501 cgagatcgtg atcggaagct tgggttatgg ttgcgcccat cgattcgatg aatttgccat
    13561 ggcccacttg ctcgcgacgt ggcatggcgg gatcactctt gcgccgggcg actccattgc
    13621 cacgccgcct cgcggtggcg cttcgcgctg ggccctgcgg gccacccttc gttccgaagg
    13681 ggtggcaacc gctgcgccgc cgcctaaccc cggatgtcgg gtcccggccc cggtgtcacg
    13741 gcagcggcac ccgctccagc aactggctga tgacgtcgtc gacgtgcagg ccctccgcct
    13801 cggcttcata gccgagcaac acgcggtggc gcaatgcgtc cggcgcgatc gactggatat
    13861 cctcgggcaa tacgtagtcg cggccggcaa gccacgccgc ggcgcgggcg caccgttcca
    13921 gcgcaatgct cgcgcgcggg ctcgcgcccc aggcgatctt ttcgcgcaac ttcggcgccc
    13981 acggcctcgg atcgcgggtg gcgagcacca gttgcacgat gtattcctcg aggccctcgg
    14041 ccatgtgcag gtcgagcacc tgttcgcgcg ccgcgaagat gtcggattgg ccgagcagcc
    14101 ggtccggcgg ctgatgccgg cccagttcct cacgcgcgcg cccgcgggcc agccgcatga
    14161 tcgcccgttc cgatcccgat tccgggtagc gcaccggcac gtgcagcagg aaacggtcga
    14221 gctgcgcttc gggcagcggg aaggtgcctt cctgctcgat ggggttctgg gtggccatca
    14281 ccaggaacag cctgggcagc ggccacgaac gccggccgat cgtc
//
LOCUS       DASSNH010000179         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_647316, whole genome shotgun sequence.
ACCESSION   DASSNH010000179 DASSNH010000000
VERSION     DASSNH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647316"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2401
                     /locus_tag="VFK00_06750"
     CDS             <1..2401
                     /locus_tag="VFK00_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7592683.1"
                     /translation="GTRLAFGQPLSTRLHMDRARVVVALDADPLSDPAAGTRYGHDYA
                     ITRSPEQNDGAMSRLYAVEPTPSLTGLMADHRLPLGFREVGEFARQLAGKLGVTTRAG
                     AAPATGEQTRWLDAIGGDLLANRGASLVLVGASQPPWLHALGHAMNAALGNVGRTIDY
                     SAPVETLPVEDDGLAGLVSAMRAGRVDTLLALEVNPVYDAPAELEFADALKRVPHLLH
                     LGLYLDETGEVAEWHIPQAHPLESWSDARAFDGTASIAQPLIAPLYDGKSVHEVLAAF
                     TGEGLFEGHALVRRQWQKALPDDQSWNDALQAGVIADTALPAVQAAARADFLTHPEVP
                     VPMSNTLPSSPRRRGSSDSDPAEATKTLDSRLRGNDEPEQQAVGDGNRIELLFRPDPT
                     IGDGRWANNGWLQELPKPLTQLTWDNAALISPALAAERKLENGDMIELRANGRKLEVP
                     VWIVPGQATRTITLHLGYGRRRAGHVGDGRGFDAHVLQASKSPGWIGDVAIARTGRYY
                     PLANTQHHFNMEGRDLVREGTLAEFRHDPHFATAHDRYPEDPPSLYPDYPPGEYAWGM
                     SIDLNACIGCKACTIACQAENNIPVVGKDQVRMGREMHWIRVDHYHVGGADNPRTVSQ
                     PVPCMMCEHAPCEVVCPVGATVHDSEGLNLQVYNRCVGTRFCSNNCPYKVRRFNFLQY
                     ADKTTPQLKAMRNPEVSVRRRGVMEKCTYCIQRIETAHIQADKESRRIRDGEVLTACQ
                     AVCPTKAIRFGDISDKQSDVANAKDSPRNYAMLNELGTRPRTTYLARLRNPNPALEGK
                     S"
     gene            2508..>3529
                     /gene="nrfD"
                     /locus_tag="VFK00_06755"
     CDS             2508..>3529
                     /gene="nrfD"
                     /locus_tag="VFK00_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7592684.1"
                     /translation="MSTVFRPEPQPPVIAPGEDALSVTRSVSGVVLKLPIGLRWMGSL
                     GFAVLLTGIFLVGICWLLYAGVGIWGLNIPVAWAFAITNYVWWIAIGMGGTFISAAMY
                     VTRQHWRASINRLAESMTVFAVAVSGLFPIFHLGRPWFFYWLFPYPDIMDLWPQWRSS
                     LEWDFWAILAYLIVSILFLYAGMLPDLATLRDRARRRLVAIFYGLLSLGWRGEAQHWR
                     RLESVTLMLAGLSVPLVFSVHSMVALDFSEGLVPGWHSTIFPPYFIAGALFSGFAMVL
                     VLAIPMRRLFGLQAYLTDRHISVIAKLMLAAGLWIDYSYASEIFTVFYSMDKFDVRME
                     MNRFLGP"
BASE COUNT          573 a         1154 c         1196 g          606 t
ORIGIN      
        1 cggcacacgg ctcgcgttcg gacaaccctt gtcgacgcgg ctgcacatgg accgggcgcg
       61 ggtggtggtg gcgctggatg ccgaccctct gtcggatccc gcggccggca cgcgctacgg
      121 gcacgactac gccatcaccc gctcgcccga acagaacgac ggcgcgatgt cgcgcctgta
      181 cgccgtcgag ccgacgcctt ccctgaccgg gttgatggcc gaccatcggc tgccgctggg
      241 atttcgggag gtcggcgaat tcgcgcggca actcgccgga aagctcggtg tcacgacgcg
      301 ggcaggtgcc gcgcccgcga caggcgaaca gacgcgctgg ctcgatgcga tcggtggcga
      361 cctgctcgcg aatcgcgggg cgtcgctggt tctggtgggt gcgtcgcaac caccgtggct
      421 gcacgcgctg ggtcacgcga tgaacgcggc gctcggcaac gtgggaagga ccatcgacta
      481 cagcgcgccg gtcgaaacgc ttcccgtgga agacgacggc ctcgccggtc tcgtgtcggc
      541 aatgcgtgcg ggcagggtcg acaccttgct ggcactggag gtcaatccgg tgtacgacgc
      601 gccggcggag ctcgaatttg cggacgcgtt gaagcgcgtt ccgcacctgc tgcatctcgg
      661 tttgtatctc gacgaaaccg gcgaggtcgc cgaatggcac atcccgcagg cgcacccgct
      721 tgagtcgtgg tcggatgcgc gcgcattcga tggcaccgcc agcatcgccc agccgctgat
      781 cgcgccgctc tacgacggca agtccgtgca cgaggtcctg gccgcgttca ccggtgaagg
      841 cctgttcgag ggccatgcgc tggtgcgccg gcaatggcaa aaggcgctgc cggacgacca
      901 gtcctggaac gatgcactgc aggcgggcgt gattgccgac accgcattgc cggcggtgca
      961 ggctgcggcg cgcgccgatt tccttaccca cccggaagtg ccggtgccga tgtcaaacac
     1021 actgccgtca tccccgcgga ggcggggatc cagcgactcg gaccctgccg aggcgacaaa
     1081 gacactggat tcccgcctgc gcgggaatga cgagccggag caacaggccg tcggtgacgg
     1141 caaccgaatt gagttgttgt tccggcccga ccccaccatc ggcgatggcc gctgggccaa
     1201 caacggttgg ctgcaggaac tgcccaagcc cttgacgcaa ctcacctggg acaacgccgc
     1261 gctgatctcg ccggcgctcg cggccgagcg caagctcgag aacggcgaca tgatcgaatt
     1321 gcgggcgaat ggccgcaagc tcgaggtgcc ggtgtggatc gtgccggggc aggcgacgcg
     1381 caccatcacg ctgcatctcg gctacggccg acgacgcgcg gggcatgtcg gcgacgggcg
     1441 cggattcgat gcccacgttc tgcaggcttc gaaatccccc ggctggatcg gggacgtcgc
     1501 gattgccagg acgggccgct actacccgct ggccaacacc cagcaccact tcaacatgga
     1561 aggccgcgac ctggtgcgcg aggggacgct cgccgagttc cggcacgacc cgcatttcgc
     1621 gaccgcgcac gaccgctatc cagaggatcc gccgagcctg tatcccgatt atccgccggg
     1681 cgaatacgcc tggggcatga gcatcgatct caacgcgtgc atcggctgca aggcctgcac
     1741 gatcgcgtgc caggccgaga acaacatccc ggtggtgggc aaggaccagg tccggatggg
     1801 ccgcgagatg cactggatac gggtcgacca ttaccacgtg ggcggcgcgg acaatccgcg
     1861 cacggtgtcg cagcccgtgc cctgcatgat gtgcgagcac gcgccctgcg aagtcgtgtg
     1921 cccggtcggg gccaccgtgc acgattcgga aggcctgaac ctgcaggtct acaaccgctg
     1981 cgtcggtacg cgcttctgtt ccaacaactg cccgtacaag gtgcgccgct tcaacttcct
     2041 gcagtacgcg gacaagacga cgccgcagtt gaaggcgatg cgcaacccgg aggtgagcgt
     2101 ccgccggcgc ggcgtgatgg agaagtgcac ctactgcatc cagcgaatcg aaacggcgca
     2161 catccaggcc gacaaggaga gccggcggat ccgtgacggc gaagtcctga ccgcctgcca
     2221 ggcggtatgt ccgaccaagg cgatccggtt cggcgacatc tcggacaagc agtcggacgt
     2281 cgcgaatgcc aaggactcgc cgcgcaacta cgcgatgctc aacgaactcg gtacgcgtcc
     2341 gcgcacgacc tatctcgcgc gcctgcgcaa tcccaatccg gcgttggagg gcaagtcgtg
     2401 atgagccgcg cgacataccg cgcgttccaa aagccctctc ccccacgcct tgtgtggggg
     2461 agcgggttgg gtgagggggc ggggctcgca ggcagaggca tccgcgcatg agcaccgtgt
     2521 tccgcccgga gccgcagccg ccggtgatcg cgccgggcga agacgcattg tcggtcacgc
     2581 gctcggtcag cggggtggtg ctgaagcttc cgatcggctt gcgctggatg ggaagcctcg
     2641 gcttcgcggt gcttctgacc gggatcttcc tggtcgggat ctgctggctg ctgtatgccg
     2701 gcgtcgggat ctggggcctc aacatccctg tcgcctgggc gttcgcgatc accaactacg
     2761 tgtggtggat agcgatcggc atgggcggca ccttcatttc ggcggcgatg tacgtgaccc
     2821 gccagcactg gcgcgcctcg atcaaccggc tggccgaatc gatgacggta ttcgcggtcg
     2881 cggtgtcggg actgttcccg atcttccatc tcggacggcc gtggttcttc tactggctgt
     2941 tcccgtatcc cgacatcatg gacctgtggc cgcagtggcg cagctcgctg gaatgggatt
     3001 tctgggcgat cctcgcctac ctgatcgtct cgatcctgtt tctgtacgcg gggatgctgc
     3061 cggacctcgc gaccttgcgc gaccgcgcgc gtcggcggtt ggtcgcaatt ttctacggcc
     3121 tgctttcgct cggctggcgc ggcgaggcgc agcactggcg gcgcctggaa agcgtgaccc
     3181 tgatgctcgc agggctgtcg gtgccgctgg tgttttcggt gcacagcatg gtggcgctgg
     3241 acttctccga aggcctggtg cccggctggc attcgacgat cttcccgccg tacttcatcg
     3301 ccggcgcgct gttctccggc ttcgcgatgg tgctggtgct cgcgatcccg atgcgccggc
     3361 tgttcgggct gcaggcctac ctcaccgacc ggcacatctc ggtgatcgcc aagctgatgc
     3421 tggccgcggg tttgtggatc gactacagct acgcctcgga aatctttacc gtgttctaca
     3481 gcatggacaa gttcgacgtg cggatggaga tgaaccgctt cctcggtcc
//
LOCUS       DASSNH010000180         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_798844, whole genome shotgun sequence.
ACCESSION   DASSNH010000180 DASSNH010000000
VERSION     DASSNH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798844"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFK00_06760"
     CDS             complement(<1..201)
                     /locus_tag="VFK00_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HET7592685.1"
                     /translation="MTSQTESRPAATPVTRPPSFATLRRRVLAALDELKAHDIREIDV
                     RGRTSIADLLIIASGTSSRHVKS"
     gene            complement(288..1007)
                     /gene="nadD"
                     /locus_tag="VFK00_06765"
     CDS             complement(288..1007)
                     /gene="nadD"
                     /locus_tag="VFK00_06765"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7592686.1"
                     /translation="MRPLAIFGGTFDPVHIGHLRAAWEAAGMLDAEVRLVPARVPPHR
                     PQPVASAAQRAAMLRAALAGQDRLALDLRELDREGPSFTFDTLTGLRGEVGALRPLVL
                     LIGADAFAGLASWHRWRELFDLAHMCVLTRPGQIPAMAGDLAGEVAARVVEGVGDLRS
                     SPAGHVLNMIVTSLGISATRIRDELANGGDPRWLVPQALLDDPALLAPYRATREAGTG
                     NWEPGTTAEDSSPLPVPRSRS"
     gene            complement(1122..2171)
                     /gene="holA"
                     /locus_tag="VFK00_06770"
     CDS             complement(1122..2171)
                     /gene="holA"
                     /locus_tag="VFK00_06770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7592687.1"
                     /translation="MAAELADFLRILAKGDLAPVYLLGGEEPLQLQEAGDAVRARARE
                     LGFTERDVLDVEPHFDWDRLARSGASLSLFASRRLIELRLPTGKPDRDGAAAITEWCK
                     APPPDTVLLISAMQWSRKHEGAWVKTVERTGKYARLQAPRVDQLPDWITARMRTHGFV
                     PTDDAVALLASRAEGNLLAIAQEIDKLAVLRGPGPVAVEELESLGDDNAIYDVFRLTD
                     AAFGGDAPRALRVLAGLRAEGEDVIPMLGWLLNQLDLALRLASAANFAEAARAEYNFW
                     PARQRLFAGALKRAGRGAAHWRRCLAWCARIDRMSKGREQDASGKAMGDPWRELERLL
                     VAIAQPRSANLLLAA"
     gene            complement(2175..2723)
                     /gene="lptE"
                     /locus_tag="VFK00_06775"
     CDS             complement(2175..2723)
                     /gene="lptE"
                     /locus_tag="VFK00_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET7592688.1"
                     /translation="MRIQKWLVLLACLATAAALAGCGFHLRRSAALPAPMAQRVYLKV
                     NGGGEFPRSMAAALRASKVEVLDEPTDGVATLSVPVADFSSNLLTTSGFQRVGEYVVG
                     FRVRFQLTDGNGNTVVPAQTISLSHEFAVDRTQFSAISSETEAIQRSLVREMTDAVMR
                     RLEARAAHPGAPGEPASAGSAG"
     gene            complement(3017..>3719)
                     /locus_tag="VFK00_06780"
     CDS             complement(3017..>3719)
                     /locus_tag="VFK00_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7592689.1"
                     /translation="DPQTMIDKYGADTVRLFSMFASPPDQSLEWNEAGVEGMARFLRR
                     FWREVVTHASQPDHPGIDPAVLDSKQRTFRRQVHETIAKVGDDIGRRRNFNTAIAALM
                     ELLNAVARFDDMGDAGRALRHEALEAMVLLLNPFTPHASHALWQVLGHHETLLEDVPW
                     PRADPAALVRDTVTLAVQVNGKLRGTLEASPDAPREQLEADARALEGVVAAVGDKSVR
                     KVIVVPGKIVNIVAG"
BASE COUNT          593 a         1301 c         1255 g          570 t
ORIGIN      
        1 cgatttgacg tggcgggagg aggtgccgga ggcgatgatc agcaggtcgg cgatggaggt
       61 gcgaccgcgc acgtcgattt cgcggatgtc gtgcgctttc agttcgtcga gggcggcgag
      121 gacacgccgg cgcagggtcg cgaagctggg agggcgggtg acgggggtcg cggcagggcg
      181 cgattcggtc tggctggtca agttgtggaa ggcctcggcc tcgagatggc gcgcggattg
      241 cgcgggtcca gtatgcacca ctcggattcg agcgcaagta tggccattca agagcgggaa
      301 cggggaacgg gcagcgggga agaatcctcc gccgtggttc ccggttccca gttccctgtt
      361 cccgcttcac gcgtcgcacg gtacggcgcc agcaatgccg gatcgtccag cagcgcctgc
      421 ggcaccagcc agcggggatc gccaccgttg gcgagttcat cgcggatgcg ggtggccgaa
      481 atgcccagcg aagtcacgat catgttgagg acatggccgg ccggcgacga acgaaggtcg
      541 cccacgccct cgacgacgcg cgcggccacc tcgccggcga gatcgccggc catcgccggg
      601 atctgtcccg gccgggtcag gacgcacatg tgcgcgaggt cgaacaattc gcgccaccgg
      661 tgccagctcg cgagccccgc gaacgcatcg gccccgatca gcaacaccag cggtcgcagt
      721 gcgccgacct cgccgcgcaa gccggtcagg gtgtcgaaag tgaacgaggg cccctcgcgg
      781 tcgagttcgc gcaggtccag cgccaggcga tcctgccccg ccagtgccgc ccgcaacatg
      841 gccgcgcgct gcgcggcgct ggcgaccggc tgcggccgat gcggcggaac ccgcgcgggc
      901 accagccgca cctcggcgtc aagcattccg gcggcttccc acgccgcacg caggtggccg
      961 atgtgcaccg gatcgaaggt gcctccgaag atggcgagcg gccgcacggt gcccatcaaa
     1021 gacaacaacg gtcggcggat gaatgcagat gaacgcaaat ggacaaggca gctattgtct
     1081 tgttcgcgtt cattgcgttc agttgctggc cggttccggt ttcatgcagc cagcagcagg
     1141 ttcgcgctgc gcggctgggc gatcgccacc agcaggcgct cgagttcgcg ccacggatca
     1201 cccatggctt ttcccgaagc gtcctgttcg cggcccttgc tcatgcgatc gatccgggcg
     1261 caccacgcga ggcagcgccg ccaatgggca gcgccgcggc cggctcgttt cagcgccccc
     1321 gcgaacagcc gttgccgcgc gggccagaag ttgtactcgg cgcgcgccgc ctccgcgaag
     1381 ttcgcggccg aagcgagacg caacgccagg tcgagctggt tcagcagcca gccgagcatc
     1441 gggatcacgt cctcgccctc ggcgcgcaga ccggcgagca cccgcagcgc gcgcggcgcg
     1501 tcgccgccga aggccgcgtc ggtgagccgg aacacgtcgt agatcgcatt gtcgtcgccg
     1561 agggattcca gttcttccac ggctaccggg cccggcccgc gcaacaccgc gagcttgtcg
     1621 atctcctgcg cgatcgcgag caggttgccc tcggcgcgcg acgcgagcag cgccacggcg
     1681 tcatcggtcg gcacgaagcc gtgggtgcgc atgcgcgcgg tgatccagtc cggcagttga
     1741 tcgacgcgcg gcgcctgcag ccgcgcgtat ttccccgtcc gctccacggt cttcacccat
     1801 gcgccctcgt gcttcctgct ccactgcatc gcgctgatca acagcacggt gtcggggggg
     1861 ggagccttgc accattcggt gatcgcggcc gcgccatcgc gatccggctt gcccgtcggc
     1921 aggcgcaatt cgatcagccg gcgcgacgcg aacagcgaaa ggctcgcgcc gctgcgggcc
     1981 agccgatccc agtcgaagtg cggttcgaca tccagcacgt cacgctccgt gaagccgagt
     2041 tcgcgcgcac gcgcgcgcac cgcgtcgccc gcttcctgca attgcaacgg ttcctcgccg
     2101 cccaggagat acaccggcgc gaggtcgccc ttcgcgagga tccgcaggaa gtcggcgagt
     2161 tcggccgcca cgactcagcc ggcgctgccc gcggaggccg gctcgccggg cgcgcccggg
     2221 tgcgccgcac gggcttcgag ccgccgcatc accgcgtcgg tcatctcgcg caccaggctc
     2281 cgctggatcg cctcggtctc actcgaaatg gccgagaact gggtccggtc gaccgcgaat
     2341 tcgtgggaca ggctgatggt ctgcgcgggc accaccgtgt tgccgttgcc gtccgtcaac
     2401 tggaaccgta cgcgaaagcc caccacgtat tcacccaccc gctggaagcc gctggtggtc
     2461 aacaggttgg acgagaaatc cgcgaccggt accgacagcg tcgccacgcc atccgtcggt
     2521 tcgtccagca cttcgacctt gctggcgcgc aacgcggccg ccatcgagcg ggggaattcc
     2581 ccgccaccgt tcaccttcag ataaacgcgc tgcgccatcg gcgcaggcag cgccgcactg
     2641 cgacgaaggt ggaacccgca accggcgagc gcggcggcgg tggcgaggca tgccagcagg
     2701 accagccact tttggatccg catcgaatca gctccggttg caccgtggat ccggatgtcg
     2761 tggcccggac gccgccgcaa catcaccaac atcgtcattc cggggcggtc gccggcttca
     2821 ttggcgatcg aagccggaac caagctcctg gcgctcgaga acccctggac atggattccg
     2881 ggttccgcat ccgatgaatc cgggtgcggt cccggaatga cgaccagggg agtcggtctc
     2941 cccgagtccc gagtccccga tcgcgagtcc cgggtccgcg caggatagca gtgccaacgc
     3001 agcacacggg acacgctcag cccgcgacga tgttgacgat ctttcccggc accacgatca
     3061 ccttgcgcac gctcttgtcg ccgactgcag cgacgacacc ctccagcgcg cgggcgtccg
     3121 cctcgagctg ttcgcgcggc gcatcgggcg aggcttccag cgtgccgcgc aatttgccgt
     3181 tcacctgaac ggccagcgtc accgtatcgc gtaccagagc ggccggatcg gcccgtggcc
     3241 agggcacgtc ttcgagcagc gtttcgtggt ggccgagcac ctgccacagc gcgtggcttg
     3301 cgtgcggcgt gaacgggttc agcagcaaca ccatggcctc cagcgcttcg tgccgcagcg
     3361 cgcgcccggc gtcgcccatg tcgtcgaacc tggcgaccgc gttcagcagt tccatcagcg
     3421 cggcgatcgc ggtgttgaag ttgcgccgcc ggccgatgtc gtcgcccacc ttggcgatgg
     3481 tctcgtggac ttgccggcga aatgtccttt gcttggaatc cagcaccgcg ggatcgatgc
     3541 ccggatggtc aggctgcgac gcatgcgtca cgacttcgcg ccagaaccgg cgcaggaagc
     3601 gcgccatccc ctcgacgccc gcttcgttcc actccagcga ctggtcgggc ggcgatgcga
     3661 acatcgagaa cagccgcacc gtgtcggcgc cgtacttgtc gatcatggtc tgcggatcg
//
LOCUS       DASSNH010000181         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1068105, whole genome shotgun sequence.
ACCESSION   DASSNH010000181 DASSNH010000000
VERSION     DASSNH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068105"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..401
                     /locus_tag="VFK00_06785"
     CDS             3..401
                     /locus_tag="VFK00_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HET7592690.1"
                     /translation="MERFYEKVRADPLLAPVFDTVVDDWDAHKVLMTSFWATVALRTG
                     HYRGNPLARHQPLPIGVGHFQRWLALWRETAGELLDAESAALMAGYAERIGYGMRVGM
                     GLSGHQRGRESGIPIRACRHLEIGARPASA"
     gene            446..1444
                     /gene="dusB"
                     /locus_tag="VFK00_06790"
     CDS             446..1444
                     /gene="dusB"
                     /locus_tag="VFK00_06790"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7592691.1"
                     /translation="MRIGSLPIEPPVALAPMAGVTDKPFRQLCKRLGAGLAVSEMTTS
                     DPRLWQTRKSLHRMDHAGEPDPVSVQVAGSDPAMLAEAARYNVAHGAQLVDINMGCPA
                     KKVCNAWAGSALLADEALVARICEAVVHAVDVPVTLKIRTGQVPEHRNALAIARIAED
                     AGIAALAVHGRTRSQKYEGLAEYDTIAAVKASVDIPVFANGDVDSPDKARFVLDCTKA
                     DGLMIGRAAQGRPWIFREIAHFLATGQRLPEPAIGEVREILLGHLEDLYGFYGEVMGV
                     RIARKHLGWYARDRPENAAFRAVANRAEGANEQCRLTRDYFDALEAGAESEFAAAA"
     gene            1635..>2395
                     /gene="metK"
                     /locus_tag="VFK00_06795"
     CDS             1635..>2395
                     /gene="metK"
                     /locus_tag="VFK00_06795"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7592692.1"
                     /translation="MSRYLFTSESVSEGHPDKVADRISDAVLDAILAQDKRAHVACET
                     MVKTGVAIVSGEVTTDAWVDIESLAREVILDIGYDSSTVGFDGATCGVLNLIGKQSPD
                     INQGVARKNPESQGAGDQGLMFGYACNEAPECMPAPIYYSHRLVEQQSKVRKSGKLKW
                     LRPDAKSQVTLLYNEDNTVAGLDAVVLSTQHDPSVKQSDLVEAVRETILKPVLPKKWL
                     AALPKNKVHINPTGKFVIGGPVGDCGLTGRKIIVDT"
BASE COUNT          417 a          805 c          810 g          363 t
ORIGIN      
        1 tggtggagcg cttctacgag aaggttcgcg ccgatccctt gctcgctccg gtgttcgata
       61 cggtggtcga cgactgggac gcacacaagg tcctgatgac gtcgttctgg gcgaccgttg
      121 cactgcgcac gggccactat cgcggcaatc cgctggccag gcaccagccg ttgccgatcg
      181 gcgtcgggca cttccagcgc tggctggcgc tgtggcgcga gaccgccggg gaactgctgg
      241 atgccgaatc cgccgcgctg atggccggct atgccgaacg catcggctac ggcatgcggg
      301 tcgggatggg cctgtccgga caccagcgcg gtcgcgagtc cggcatcccc atccgcgcat
      361 gccggcacct cgaaatcggc gcgcggccgg cgtctgcctg aaaagccgcg gcggcttggg
      421 atcctgccgc cgctaaactc gccggatgag gatcggatcc ctgcccatcg agcccccggt
      481 ggcgctggcg cccatggcgg gcgtcaccga caaaccgttc cggcagctct gcaagcggct
      541 gggggcgggg ctggccgtgt cggaaatgac cacctccgat ccgcggctgt ggcagacccg
      601 caagtcgctg caccggatgg accacgccgg cgaaccggat cccgtgtcgg tgcaggtggc
      661 gggttcggac ccggcgatgc tggcggaagc cgcgcgctac aacgtggccc acggcgcgca
      721 gctcgtcgac atcaacatgg gttgcccggc caagaaggtg tgcaacgcct gggcaggttc
      781 cgcgctgctg gcggacgagg cgctggtggc gcgcatctgc gaggccgtgg tgcacgcggt
      841 ggacgtgccg gtaacgctca agatccgcac cgggcaggtc cccgaacacc gcaacgccct
      901 agccatcgcc cgcatcgccg aagacgcggg aatcgccgca ctggcggtcc acggccgcac
      961 ccgttcgcag aaatacgagg gtttggccga atacgacacg atcgcggcgg tgaaggcatc
     1021 ggtggacatt ccggtgttcg ccaacggcga cgtcgattcg cccgacaagg cgcgcttcgt
     1081 gctcgactgc accaaggccg atggcttgat gatcggccgc gccgcgcagg ggcgcccgtg
     1141 gatcttccgc gagatcgcgc acttcctcgc cactggccag cgcctgccgg aaccggcgat
     1201 cggcgaagtg cgcgagatcc tgctcggcca cctcgaagac ctgtacgggt tctacggtga
     1261 agtgatgggc gtcaggatcg cgcgcaagca cctcggctgg tacgccaggg accggcccga
     1321 gaacgcggcg ttccgcgccg tggccaaccg cgccgaaggc gccaatgagc aatgccgcct
     1381 gacccgggac tatttcgatg cgctggaagc gggcgcagaa tccgaattcg ctgcagccgc
     1441 gtgaccaccc cccttcggaa ccaagggggt cgcgcacatg ggtgcgcggg gggatgccgt
     1501 cgctccccct tcgcaagcga agggcgaagc aacagcctga acgcgcgacg ccggccgctg
     1561 catcctcccc ttcgtccttg tatgatgctc cgttcattcc atccttccat ccgtatcaag
     1621 ggatataaac gccgatgagc cgctacctgt tcacctccga atcggtttcc gaaggccacc
     1681 cggacaaggt cgccgaccgg atttccgatg ccgtgctgga cgcgatcctg gcgcaggaca
     1741 agcgcgcgca cgtggcctgc gaaaccatgg tcaagaccgg cgtcgcgatc gtctccggcg
     1801 aggtgaccac cgacgcgtgg gtggacatcg agtcgctggc gcgcgaggtg atcctcgaca
     1861 tcggctacga ctcctcgacc gtcggcttcg acggcgcgac ctgcggcgtc ctgaacctga
     1921 tcggcaagca gtcgcccgac atcaaccagg gcgtggcgcg caagaatccc gaatcgcaag
     1981 gtgcgggcga ccagggcctg atgttcggct acgcgtgcaa cgaggcgccc gaatgcatgc
     2041 cggcgccgat ctactactcg caccggctgg tcgagcagca gtcgaaggtg cgcaagagcg
     2101 gcaagctgaa atggctgcgg ccggacgcca agtcccaggt gacgctgctg tacaacgaag
     2161 acaacaccgt cgcgggcctc gatgcggtgg tgctctcgac ccagcacgat ccgtcggtca
     2221 agcagtccga cctcgttgaa gcggtgcgcg agaccatcct gaagccggtg ctgccgaaga
     2281 agtggctggc cgcgctgccg aagaacaagg tgcacatcaa cccgaccggc aagttcgtga
     2341 tcggcggccc ggtcggcgac tgcggactga ccggccgcaa gatcatcgtc gacac
//
LOCUS       DASSNH010000182         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1137294, whole genome shotgun sequence.
ACCESSION   DASSNH010000182 DASSNH010000000
VERSION     DASSNH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137294"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..913)
                     /locus_tag="VFK00_06800"
     CDS             complement(26..913)
                     /locus_tag="VFK00_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HET7592693.1"
                     /translation="MNQAEGLDRIMQELKNNAQNPPPRRPPRVIAVTGGKGGVGKSCV
                     SVNLGLALAMAGHDTLLLDADLGLANVDVLLGLQPKCNIANLLAGECSLGDLVLTAAH
                     GLKVVPATSGKRSMVDMTRTEYAAVIRAFDEYPQPPEFLIVDTAAGISDNVSMFSVAA
                     DEVLVVVCDEPASLTDAYALIKVLARESGVRRFRVVANMVYNARHGQQLFEKLERVTN
                     RFLDVSLQFCGVVPQDEHLRRAVRRQMGVVDAWPDSPSALAFKELAVAADNWEESPEA
                     LGRIGFFAERRMSRGEQAA"
     gene            complement(931..>2161)
                     /gene="flhF"
                     /locus_tag="VFK00_06805"
     CDS             complement(931..>2161)
                     /gene="flhF"
                     /locus_tag="VFK00_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET7592694.1"
                     /translation="AAESSTMKIKRFVAPAMRQAMQQVRDDQGPDAVILSTRRCEEGI
                     EIIAAIDYDEALVREAAVHAAPIPAAPKPAPRARETIDEQQESTYAPPSRNGGEPSPA
                     ISDMRQELGNLREMLETQLASLAWNDMNRRHPIQASVLRQLTRMDMDADIASAVADEL
                     PAGLSAEQARYLPLGLIAGRIATGGQQLSTLAGVVAMVGPTGVGKTTTVAKLAAHAVL
                     HHGAARVALISTDHYRIGAGAQLGHYARLLGVPVYEVHQPGELRPLLARLKDRSVVLV
                     DSAGMGAHDPRLVGQLDILRTAQPDMRSTLVLAANAQARAMDEAVRAYLPLAPAGCIL
                     TKLDEAPQLGGALSVLIRHGLRLDFTTDGQRVPEDIAAADVRQLVCRAARNHSAGHAG
                     SDIDMAERFGRLAGAGA"
BASE COUNT          286 a          753 c          752 g          370 t
ORIGIN      
        1 acctgcaggt attcggcact cgcgttcatg ccgcctgttc cccccggctc atgcggcgtt
       61 ccgcgaagaa cccgatccgg ccgagcgctt cgggcgattc ctcccagtta tcggcagcta
      121 ccgccagttc cttgaatgcc agggccgagg ggctgtccgg ccaggcgtcg accacgccca
      181 tctggcggcg gaccgcgcgg cgcaggtgct cgtcctgggg cacgaccccg cagaattgca
      241 ggctcacgtc gaggaagcga tttgtgacgc gttcgagctt ttcgaacaat tgctggccgt
      301 ggcgggcgtt gtagaccatg ttggccacca cccggaagcg gcgcacgccg ctttcgcgcg
      361 ccagcacctt gatcagcgcg taggcgtcgg tgagcgaagc cggctcgtcg cagaccacca
      421 ccagcacctc gtcggcggcg acgctgaaca tcgagacgtt gtcggaaatc cccgcggcgg
      481 tgtcgacgat caggaattcc ggcggctgcg gatactcgtc gaacgcgcgg atcaccgcgg
      541 cgtattccgt gcgcgtcatg tccaccatgc tgcgcttgcc ggaggtggcc ggtaccacct
      601 tcaatccgtg cgcggcggtg agcaccaggt ccccgaggct gcattcgccg gccagcaggt
      661 tggcgatgtt gcacttgggc tgcaggccca gcagtacgtc cacgttggcg aggccgaggt
      721 cggcgtcgag cagcagggtg tcgtgcccgg ccatcgccag tgccagtccc aggttcaccg
      781 aaacgcacga tttcccgacg ccgcccttgc cgccggttac ggcgatcacc cgtggcggcc
      841 gccgcggcgg cgggttctgc gcgttgttct tcaattcctg catgatgcga tccagacctt
      901 ctgcctgatt catgtcgcgg tttcctgctc tcacgccccg gcgccggcca ggcgcccgaa
      961 gcgctcggcc atgtcgatgt cgctgccggc gtggcccgcg gaatggttgc gggccgcgcg
     1021 gcagaccagt tgccgcacgt cggcggcggc gatgtcttcc ggcacgcgct ggccgtcggt
     1081 ggtgaagtcc aggcgcaagc cgtggcggat cagcaccgag agcgccccgc ccagttgcgg
     1141 ggcttcgtcc agcttggtga ggatgcagcc cgcgggcgcg agcggcaggt aggcccgcac
     1201 cgcttcgtcc atcgcacgcg cttgggcgtt ggccgccagc accagcgtcg agcgcatgtc
     1261 cggctgcgcc gtgcgcagga tgtcgagttg ccccaccagc cgcgggtcgt gcgcgcccat
     1321 gccggcgctg tcgaccagca ccacgctccg atccttgagg cgcgccagca gcgggcgaag
     1381 ttcgccgggc tggtgcacct cgtagaccgg cacgcccagc agccgcgcgt aatggccgag
     1441 ctgggcgccc gcgccgatcc ggtagtggtc ggtgctgatc aatgccaccc gggccgcccc
     1501 gtggtgcagg accgcgtgcg cggcgagctt ggccaccgtg gtggtcttgc ccacgccggt
     1561 ggggccgacc atggccacca cgccggccaa ggtcgacaac tgctggccgc cggtggcgat
     1621 ccgccccgcg atcaggccga gcgggagata gcgcgcctgt tcggccgaaa ggccggccgg
     1681 cagctcgtcg gccaccgcgc tcgcgatgtc ggcatccatg tccatccggg tgagctggcg
     1741 cagcacgctg gcctggatcg ggtggcgccg gttcatgtcg ttccaggcga ggctcgcgag
     1801 ctgcgtctcc agcatctcgc gcaggttgcc gagttcctgg cgcatgtcgc tgatcgccgg
     1861 cgaaggttcg ccgccgtttc gactgggcgg cgcgtacgtc gattcctgct gttcgtcgat
     1921 cgtctcccgc gcgcgcggcg caggtttcgg cgcggcggga atgggcgccg cgtgcaccgc
     1981 cgcttcgcgc accagtgcct cgtcgtagtc gatggcggcg atgatctcga tgccttcctc
     2041 gcagcgccgc gtggacagga tcaccgcatc ggggccctgg tcgtcgcgga cctgctgcat
     2101 ggcctggcgc atcgccggtg ccacgaatcg cttgatcttc atggttgatg actccgcggc
     2161 g
//
LOCUS       DASSNH010000183         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1262126, whole genome shotgun sequence.
ACCESSION   DASSNH010000183 DASSNH010000000
VERSION     DASSNH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262126"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..75
                     /locus_tag="VFK00_06810"
     CDS             <1..75
                     /locus_tag="VFK00_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7592695.1"
                     /translation="SGAQFVIHNPNAKTTCGCGSSFTA"
     gene            231..1154
                     /gene="nudC"
                     /locus_tag="VFK00_06815"
     CDS             231..1154
                     /gene="nudC"
                     /locus_tag="VFK00_06815"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET7592696.1"
                     /translation="MSSAERSRLNTFANTPLDRCGEFRGDGAWLAEQAAAPDARWLGC
                     DAEGRAPVRDGRLCWLDAATAGPGASANFLGRLADAPIFSLRGAIRHESFPDAAWLDL
                     RSAAVGLAGDEAGLFAYARGLANWQQATRYCPACGTELRLVEAGHRAHCASCDRLHFP
                     RTDPAIIVIVEHEDACLLGRQAHWHPNQFSTLAGFVEPGESLADAVRREVREETGIEV
                     VDCDFHSSQPWPFPVSLMLGFTATAAGRAITLADGELEDARWFTADDIVAGLGDGSLR
                     ASTRFSISYRLLEHWLRTRAGIELSVLPHAE"
     gene            1200..2072
                     /locus_tag="VFK00_06820"
     CDS             1200..2072
                     /locus_tag="VFK00_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase induction protein"
                     /protein_id="HET7592697.1"
                     /translation="MAIELVAALIVLLVLLLWPGNVAGLRRFGWYRNWLGVLDFVEGG
                     GRVALALVLPVAACAVVAHFLEGRVYALAALAFAVLILFYTFGPRELAADFDAVLNED
                     DVVARVVAAQHLRSEPDGAPRAFTSAELVEAAVMAALRRRFGILFWFFLLGPAGALGY
                     RLAWVTADNGDGRIDPRTRYAARRFAFALDWVPAHLMVLAMALVSNFDAVIGTWRRWH
                     GHPARSPWDFDPGFLAAVARSGVDADITAGDGATTDIRDPIVELADARRLMLRVLIVW
                     LAVVALIVLAGWVT"
     gene            complement(2201..2905)
                     /locus_tag="VFK00_06825"
     CDS             complement(2201..2905)
                     /locus_tag="VFK00_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET7592698.1"
                     /translation="MRILVADDEPLARARLGALLRECEGAEVVASVGDGETALAACVE
                     TQPDLLLLDIAMPGVDGIGVARRLACMSNPPQLVFCTAYEEHALAAYELRAADYLLKP
                     VRLERLREALARAQALRQRKPAPDATLLVQVHGAPVRIRLSEVSHLSADDKYVTLHRE
                     AGDALTEQSLKSIEEAFPDRFVRVHRGCLIPVERLLGLRREADGSVHALIAGCEATPE
                     VSRRNLPAVRKLLRGD"
     gene            complement(2886..3929)
                     /locus_tag="VFK00_06830"
     CDS             complement(2886..3929)
                     /locus_tag="VFK00_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7592699.1"
                     /translation="MVESMIRDEREAATLPDFCSMASVFTLVIVAELVVVIDALAPDA
                     RMHWRGFATATLFVVWLALLSSLMLCRLVPRLRRVSQVPAYLLALAALVSLVVAASLV
                     VAWLDHALGTGLTPVSMPRFVVGNAVLAALLGAALLRYFYVLAEWRARLAAVARAQFE
                     ALQARIRPHFLYNSMNTVAALVRVDPAAAERTVEDLADLFRAALGAEDKPSTLGAELG
                     LIDRYLAIEQLRLGDRLKVERELAALPKDMELPALLLQPLVENAVYHGIQPRRDGGTL
                     RIAGRCSDDGVEIEIDNPLPDAASPPRSGHGMENVRRRIAYHFGESGRLDVRIEDGVH
                     RVIVELPRAHPGC"
     gene            complement(4105..4764)
                     /locus_tag="VFK00_06835"
     CDS             complement(4105..4764)
                     /locus_tag="VFK00_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7592700.1"
                     /translation="MTLECVEVETRPQPTHAVIWLHGLGADGHDFEPLVPQLLRSGWP
                     ALRFVFPHAAIRPISLNNGLPMRGWYDIAGFDLSQRQDETGVRASITEVEALIEREAA
                     HGIAADRVVLAGFSQGGAVALAAGLRHARKLAAIVALSTYLPVAEAIGRERSEANASI
                     PIFMAHGAFDPVVPKVLGERSRDRLRGWGYSVAWHDYPMAHQVCPQEIADLADFLAGV
                     W"
     gene            complement(4761..>6200)
                     /locus_tag="VFK00_06840"
     CDS             complement(4761..>6200)
                     /locus_tag="VFK00_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7592701.1"
                     /translation="GSDAKDDAVEFGKGLSKVAEYTLLGSVGARHAAALLHYGDGAPD
                     AVYLRDGDGPWKLALGIEANVRAASGVNHGAAWDGDRLLVIAYRDAPRGKLLALDTNG
                     QSRVLVSQGDWAMHSVAPIEGGFLVTEVRGPDWRVQQYSRDGTFVRTVPLPKSGVGVG
                     DIASSETSDRALISYSGWTVPSRWVDYDGTRGSLETVFEVKAAADYSKLETYRLDAVS
                     KDGTKVPVTVIAMKGVTPNGTRPTILYGYGGFGITTAPRFLGSTLAWLERGGVYAVAN
                     IRGGGEFGEGWHEAGMLGKKQNVFDDFHAAAEALVQHHWTDAAHLGILGGSNGGLLMG
                     SALTQHPSDYRAVVSLVGIYDMLRAELWPNGQYNISEYGTVANPADFRWLRAYSPLQN
                     VEQGTAYPAVLLVTGVNDPRVAPWQSRKFAAALQAATTSRNPILLLTRMNEGHGVTAS
                     FGQRVGNAAATLAFFAQELGLGEAVEPGQ"
BASE COUNT          939 a         2191 c         2080 g          990 t
ORIGIN      
        1 tcgggggcgc aattcgtgat ccacaacccc aacgccaaga cgacctgcgg gtgcggaagc
       61 tcgttcacgg catagccttg cgatcaatcc gtgatcgccg cgttgccagc cgggcgtcct
      121 cgcaacggcc ccgcgattac agaacgtcgg aacggccttc catggcctgg cttctcacaa
      181 cagccgcttc gaaccggccg atttgctgtc cggcttgctg ggatgacttc gtgagttcgg
      241 ccgaacgttc gcgtctcaat accttcgcca acactccgct cgatcgctgc ggcgagttcc
      301 gcggcgatgg cgcgtggctc gcggaacagg ccgcggcacc cgacgcccgc tggctcggct
      361 gcgacgcgga aggccgggcg ccggtgcgcg acgggcggtt gtgctggctc gatgccgcga
      421 ccgccgggcc gggtgcatcc gcgaatttcc tcgggcggct tgccgacgcg ccgatcttct
      481 cgttgcgagg tgcgatccgg cacgaatcgt ttcccgacgc agcctggctc gacctgcgat
      541 ccgccgccgt cggcctcgcc ggcgacgagg caggcttgtt cgcctatgcg cgcggcctcg
      601 ccaactggca gcaggcgacg cgctactgtc cggcgtgcgg cacggaactg cggttggtcg
      661 aagccggcca tcgcgcgcac tgcgcgtctt gcgaccggct gcatttcccc cgcaccgatc
      721 ccgccatcat cgtgatcgtc gaacacgagg acgcctgcct gctcggccgg caagcccact
      781 ggcatcccaa ccagttctcg accctggccg gattcgtgga gcccggcgag tcgctggcgg
      841 atgcggtgcg ccgcgaggtg cgcgaggaga ccggcatcga ggtggtcgac tgcgacttcc
      901 attcctcgca accgtggccg ttccccgtgt ccctgatgct gggcttcacg gccaccgcgg
      961 ccggccgcgc catcaccctc gccgacggcg aactggagga cgcacggtgg ttcaccgccg
     1021 acgacatcgt cgcgggcctg ggtgacggtt cgctgcgggc atcgacacgg ttttcgattt
     1081 cgtatcgcct cctcgagcac tggctgcgca cgcgcgcggg gatcgagctg tccgtgctgc
     1141 cgcacgcgga gtagcactcg ccgctacact cgccggcaat cccgcgagca gaagaacgga
     1201 tggccatcga actcgtcgcc gcgctgatcg tgttgctggt cttgctgttg tggcccggca
     1261 atgtcgccgg cctgcgcagg ttcggctggt atcggaactg gctcggcgtc ctcgacttcg
     1321 tggagggtgg cgggcgcgtg gcgcttgcac tcgtattgcc ggtcgcggcg tgcgcagtgg
     1381 tcgcgcactt cctggagggg cgcgtctatg cgctggcggc gctggcgttc gcggtgctga
     1441 tcctgttcta cacgttcggc ccacgcgagt tggcggccga cttcgacgcc gtgctgaacg
     1501 aggacgacgt cgtcgcgcgc gtggtggccg cgcagcacct gcgcagcgag ccggacggcg
     1561 cgccgcgtgc cttcacgtcc gcggagctgg tcgaggccgc ggtgatggcc gcgttgcgac
     1621 gccgcttcgg gatcctgttc tggttcttcc tgctgggccc ggcgggcgcc ttgggctatc
     1681 ggctcgcctg ggtaacggcc gacaacggcg acgggcgcat cgatccgcgt acccgctacg
     1741 cggcgcggcg tttcgcattc gccctcgact gggtgccggc ccacctgatg gtgctggcga
     1801 tggcgctggt ctccaacttc gacgcggtga tcggcacctg gcgaaggtgg catgggcacc
     1861 ccgcgcgttc gccgtgggac ttcgaccccg gcttcctcgc ggccgtggcg cgctccgggg
     1921 tcgatgccga catcactgcg ggcgacggcg cgaccaccga tatccgcgac ccgatcgtcg
     1981 agctcgccga cgcccgccgc ctgatgttgc gggtgctgat cgtgtggctc gcggtcgtgg
     2041 ccctgatcgt gctggcgggg tgggtcacgt agagccgggg aaaccgggga aaaccggggt
     2101 cagagtgcac tttcgaatac cgatacaacc ccatcgcgat gcttcgcacg aaagtgcact
     2161 ctgaccccag cttttgcctg gccttccgga ctcccggctt ctagtctccg cgcagcagct
     2221 tccgcaccgc gggcaggttg cgccggctga cttccggcgt cgcctcgcaa ccggcgatca
     2281 gcgcgtggac cgacccgtcc gcctcccgcc gcagtcccag cagtcgttcg accggaatca
     2341 ggcagccgcg gtgcacgcgc acgaagcggt cggggaatgc ctcctcgatc gacttcagcg
     2401 attgctcggt cagtgcatcg cccgcttcgc gatgcaaggt gacgtacttg tcgtctgcac
     2461 tgaggtggga gacttccgac aaccggatcc gaaccggcgc gccatgcacc tggaccagca
     2521 gcgtcgcatc cggcgcgggc ttgcgctgcc gcagcgcctg cgcgcgcgcc agtgcctcgc
     2581 gcaagcgttc gagccgcacc ggtttcaaca ggtaatccgc ggcacgcaac tcgtaggcgg
     2641 ccagcgcgtg ctcctcgtag gccgtgcaga aaaccagttg cggcggattg gacatgcagg
     2701 cgagccggcg cgccaccccg atgccgtcga cgccgggcat cgcgatgtcc agcagcagca
     2761 ggtcgggttg cgtttcgacg caggccgcca gcgccgtttc gccgtcgccg acgctcgcga
     2821 ccacctccgc accctcgcat tcgcgcagca gcgcgccgag gcgcgcgcgc gcaagcggtt
     2881 cgtcgtcagc aaccaggatg cgcacgcggc aactccacga tcacccgatg gaccccgtcc
     2941 tcgatccgga cgtccagacg tccgctctca ccgaaatggt aggcgatcct gcggcgcacg
     3001 ttttccatgc catgcccgct gcgcggtggc gaagcggcat cgggaagggg attgtcgatc
     3061 tcgatttcca cgccgtcatc gctgcagcgc ccggcgatgc gcagggtgcc gccgtcgcgg
     3121 cgcggctgga tgccgtggta gacggcattc tcgaccagcg gctgcagcag cagcgcgggc
     3181 agttccatgt ccttcggcag cgccgcgagt tcgcgctcga ccttcaggcg atcgcccagt
     3241 cgcagttgct cgatcgccag gtagcgatcg atgaggccga gttcggcgcc cagcgtgctc
     3301 ggcttgtcct ccgcgcccag cgcggcgcgg aaaagatccg cgaggtcctc cacggttcgc
     3361 tccgccgcgg cgggatcgac ccgcacgagg gccgccaccg tattcatgct gttgtagagg
     3421 aagtgcggcc ggatgcgcgc ttggagcgcc tcgaactgcg cgcgcgcgac ggcggcgagg
     3481 cgcgcgcgcc attcggccag cacgtagaag tagcggagca gtgcggcgcc aagcagggcg
     3541 gcgagcaccg cgttgccgac gacgaaccgc ggcatcgata ccggcgtcag gccggtgccg
     3601 agggcgtggt ccagccaggc gacgaccagg cttgcggcga cgaccagcga aaccagtgcg
     3661 gccagtgcga gcaggtaggc gggaacctgc gacacccggc gcagccgcgg caccagcctg
     3721 cacaacatca gcgacgacag cagcgccagc cagaccacga acaacgtcgc ggtcgcgaaa
     3781 ccccgccagt gcatccgcgc atcgggcgcg agcgcgtcga tcaccaccac caattcggcg
     3841 acgatgacca gcgtgaacac cgaggccatg ctgcagaagt ccggcagcgt ggcggcttcg
     3901 cgctcgtcgc gtatcatgga ttccaccatg ctgcgcagta tgcctgcatg tggccacgga
     3961 cgtcccgtgg ctgactatcc gcgtccaacc caccgcctat ctgttcgtcg cgggcaaagc
     4021 attgacgcgg attcacgcgg atcgccacgg atcagatgca gtatgcgcgt cgatccgcat
     4081 tcatggcttt ggcctctccg cggcctacca cacgccggcc aggaagtcgg ccaggtccgc
     4141 aatttcctgc gggcacacct ggtgcgccat cgggtaatcg tgccaggcca cggaatagcc
     4201 ccagccgcgc agccggtcgc gcgagcgctc gccgagcacc ttcggtacca ccgggtcgaa
     4261 ggcgccgtgc gccatgaaga tcggaatcga cgcgttggcc tcgctgcgct cgcggccgat
     4321 cgcctcggcg accggtaggt aggtcgacag cgcgacgatc gccgcgagct tccgcgcgtg
     4381 gcgcaacccc gccgccaggg cgaccgcgcc gccttgcgaa aagcccgcca gcacgacgcg
     4441 gtccgccgcg atgccgtgcg cggcttcccg ttcgatcagc gcctcgacct cggtgatcga
     4501 cgcgcgcacg cccgtctcgt cctgtcgctg cgaaaggtcg aagccggcga tgtcgtacca
     4561 gccgcgcatg ggcaggccgt tgttcaggct gatcggccgt atcgccgcgt gcgggaacac
     4621 gaagcgcaag gccggccaac cggaccggag caactgcggc accagcggct cgaaatcgtg
     4681 gccgtcggcg ccgagaccgt gcagccagat caccgcatgc gtcggctgcg gccgggtctc
     4741 gacctccacg cattccagtg tcattggccc ggctccacgg cttcgccgag ccccaactcc
     4801 tgcgcgaaga acgccagcgt cgccgcggcg ttgccaaccc gctgaccgaa ggacgctgtg
     4861 acaccgtggc cctcgttcat gcgggtcagc aacaggatcg gattccgcga cgttgtcgcg
     4921 gcctgcagtg ccgccgcgaa cttgcgcgac tgccacggcg cgacccgcgg atcgttgacg
     4981 ccggtgacca gcagcactgc cggataagcc gtgccctgct ccacgttctg caacggcgaa
     5041 tacgcgcgca gccaccggaa atcggcaggg ttggcgaccg taccgtactc gctgatgttg
     5101 tattggccgt tgggccacag ctcggcgcgc agcatgtcgt agatgccgac cagcgacacg
     5161 acggcgcggt agtcggaagg gtgctgggtc agagcggaac ccatcaacag cccgccgttg
     5221 gagccaccca ggatgccgag gtgcgcagca tcggtccagt ggtgctgcac cagcgcctcg
     5281 gccgccgcgt ggaagtcgtc gaacacgttc tgcttcttcc ccagcatgcc ggcctcgtgc
     5341 cagccctcgc cgaactcgcc gccgccgcgg atgttggcga ccgcgtacac gccgccgcgt
     5401 tccagccacg cgagggtcga gccgaggaag cgcggcgcgg tggtgatccc gaagccgccg
     5461 tagccgtaga ggatcgtggg cctcgtgcca ttcggcgtca cgcccttcat cgcgatcacg
     5521 gtgaccggga ctttcgtgcc gtccttcgaa accgcatcga ggcgatacgt ctcgagcttc
     5581 gaataatccg ccgcggcctt cacctcgaag acggtctcga gcgagccgcg cgtgccgtcg
     5641 tagtccaccc agcgcgaggg aaccgtccag cccgaatagc tgatcagcgc gcggtcggag
     5701 gtctcgctgc tagcgatgtc gccgacgccg acgccgctct tcggcaacgg gacggtgcgc
     5761 acgaaggttc cgtcgcgcga atactgctgc acgcgccaat cgggacctcg cacttcggtg
     5821 acgagaaatc cgccctcgat cggcgcgacc gaatgcatgg cccagtcgcc ttgcgaaacc
     5881 agcaccctgg actgcccatt cgtgtcgagc gccagcagct tgccgcgtgg cgcatcgcgg
     5941 taggcgatca ccagcagccg gtcgccatcc caggcggcgc catggttgac gccggaggcc
     6001 gcgcgcacgt tcgcctcgat gccgagcgcg agcttccagg gaccatcgcc atcgcgcagg
     6061 tacaccgcgt cgggcgcgcc gtcaccgtag tggagcagcg cggcggcatg gcgggcgccg
     6121 accgagccga gcagggtgta ctcggcgacc ttcgacaagc cttttccgaa ttccaccgcg
     6181 tcatccttcg cgtcgctgcc
//
LOCUS       DASSNH010000184        11179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_602158, whole genome shotgun sequence.
ACCESSION   DASSNH010000184 DASSNH010000000
VERSION     DASSNH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602158"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFK00_06845"
     CDS             <1..772
                     /locus_tag="VFK00_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7592702.1"
                     /translation="ADAANQQAEQITSASAKINEMAVSIDQVSRNSADSAEVAQRSVQ
                     IASNGAQIVRQTISGMDSIREQIQETSKRIKRLGESSQEIGSIVELINDIAEQTNILA
                     LNAAIQAASAGEAGRGFAVVADEVQRLAERATGATKRIETLVQTIQSDTVEAVSSMEQ
                     TTTQVVNGARLAEDAGTALGEIEKVSTNLAGLIQGISSAARQQSAAATNISSTMSSIQ
                     QITAQTSVGANQTAESIGNLAHLAQELRRSVADFKLPA"
     gene            789..6926
                     /locus_tag="VFK00_06850"
     CDS             789..6926
                     /locus_tag="VFK00_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET7592703.1"
                     /translation="MTAGDDMMSAATGEAGVAAAAPEEVAEQAVGVSVRKQAMRLEEQ
                     AEFSTLSWIKPQIDQLLSEARQSLETYAENPADRQAMQICSTLLHQVLGTLRMVELYG
                     AAELAHEMECLAQALIEDKVDDRDEAFGALMRSLVQLPDYLDLIESGHKDIPIVLLPL
                     LNELREARGCEAADARVLFHPDLDRPLPPDAAGARAPFPFADLRQRATRIRARFQAQL
                     LPWTQGKAPDFAAMHECMDELRALCHAEPARRLWWVAGGVLEALQQGRLDHHLASLRQ
                     QFGHLDRTIHRMQDQGEEVCGDEDARELTRDLLFHVVQAGPGAGRSEAIAECFALDRM
                     VPSEAELERARAAMSGRNRALLDTVAKAVREDLLRVKEGLDIYLRRDDREPDQLAPQV
                     ETLHRVCETLAVLGLDTPARMVRDQCNLVAQINDGEREARPETILDIASALLYVDASL
                     DEHIENLGGPGGSDETLPFSEGRKIMRALMHEAGANLGGVKGALTAFIESSWSHAELA
                     DVPKLLAEVTGALRILNLDAPADVVEGIDRYIGNELIADHRVPTVDEMDTLADAMASV
                     EYYLEIGRDASAGDNRILDAARRSLEALHYWPLPERRVPAYADAESGDEAAPARAAPP
                     ASGPVTVGRGLPSPVDIPVAAAQATDAGPSAAEAVPGVGPGQWIEVEEDVLEPIASEG
                     VSVETAFQEAAGIDEEIREVFIEEVGEEIANINVNLPAWKADPEDLDTLKNVRRSFHT
                     LKGSGRLVGAVALGEFSWKIENMLNRVLDRTILPDGNVLEVVEAAVATLPKLHAGLRG
                     GAIELNPPLDAITQTADKLAAGEPARIADSMGGYRKVLRKVRRWVPADEGEAPPVPLS
                     DQVVTSPESGLPVVDPILLDVLRTEVGLHLERMRGYIGRNANHDVAVDDELVRSVHTL
                     HGAVAMVGIESLARMLGPTEVWVRRVRSAGAPLDREGCDALRDAASVTEHVMAEFDAP
                     VPDLPDITALAERMEALRDRWPEAAPLGAPRAAMADAAEAIELSEEDLDGALAIGLEA
                     ASMLPTEDDEDVLAAREESGRRAEDKAEAERHAAEQADAERVAADKAEAERLAAEQSE
                     QGRAAAEQVEAERSAAEQAERDRIAAEHAEQERVAAEQAEQERIAAERAEQERIAAEK
                     AEAERVAAEQAERERIAAEQAEQERIAAEQAEQERIAAENAEVERIAAEQAEQERIAA
                     EQAEAERLAAEQAERERIAAEKAEAERIAAAREVAAREIAAAALPPFPEDPQPDGPLD
                     IPDLDPELVSLFTEEAGELLDSSDSQVTTLRHAPDDVESVRGLQRNLHTLKGGARVVG
                     IAAVGDLAHAMETLLERTGDRQRELTPIEVDSLERGFDRLHDLVQRLVQGRALADPAN
                     AIERFNALAEGRPVDGSARPDEAPAPARPAPPPRPAPLLDETEIQAPAEMIRVRSDLL
                     DSLVNAAGEASIYRARLEQQIGNFRFNLVELDQTVARLREQLRKLEIETETQILSRYQ
                     RENEAGEGATFDPLELDRFSNLQQYSRALAESVSDLASIEDILADQTRQSETLLLQQS
                     RVNSDLQDGLMRTRMVPFESMVPNLRRTLRGAADELGKRAQLRVEGAQGEMDRNVLER
                     MKAPFEHMLRNALTHGIEDPAQRVAIGKPMEGTVTIQVGRQGTEVLVRVSDDGAGFDR
                     DAIRAKAIERGLLKPEVPVSDAELLNFVLQTGFSTASEISQLAGRGVGMDVVANQIRQ
                     LGGSLTIESERGRGTVFNIRLPFTLAVTQAITVRAADTTFAIPMTSVQAVSRIRPEEL
                     VERKASGQMTVNHVGEEYTLHDLSELLGLGISPIPDEGSGQLPLLMVRAGDLRAAVRI
                     DTVIGSREIVVKPVAPQVSNVPGMFGATIMGDGSVMLILDLAPLVRHVVANRAAALAI
                     GEADAVPQEPVVEAPRSREGGPRMVMVVDDSITMRKVTTRVLEREQLEVVTAKDGVDA
                     LEKLQERVPDAMLLDIEMPRMDGYELATHMKNDARFRHVPIIMITSRTGEKHRQRALE
                     IGVERYLGKPYSEADLLHHVREMLEAGRA"
     gene            6919..8019
                     /locus_tag="VFK00_06855"
     CDS             6919..8019
                     /locus_tag="VFK00_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592704.1"
                     /translation="MPDHLIPRLPRIALVFGDQAAAAHLREALQAHVEIAYASDAGGF
                     DAARMAAANVDAALVNLDDGGGLDTFEALLDAADVPVVYNDPEISRELEGWARARWLR
                     HLLAKLRGSTDVDPPRPGQPAAGFAVPSAAASHDSRAPHHTGVFERPLSPQEIETMTA
                     DFVQESAGVPAGDMGQSAVADAPAQTAATGALADAALEPVQAHGVDDLDVDTETLSAM
                     IDARLADADSRSPSDSPEVWRLVEGGTAANAMPDAGAEPGSSVAPVVATSGAVEDSEV
                     PVSLPSLDDWQLVDTDAAPAPAPRREQQKNSDLALAESLAGLSLELEPMETVTTVVHA
                     EPIERWLHESEPRKTAASARSDAEAESIGGKA"
     gene            8016..8495
                     /locus_tag="VFK00_06860"
     CDS             8016..8495
                     /locus_tag="VFK00_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7592705.1"
                     /translation="MTTPRLRPQEIRGVMLPVTGGRLLVPNTTMAEVITYAHPAAVEG
                     APSWLLGRLTWRGWGLPVLAFSVLAGVSGGESTGNARVAILKALNGHPRLPYLGVLTQ
                     GFPRLTLVSEEMLVPDPDAVDLPTGVREQVLVHEEPAWIPDLATLEDLAAQTLTAAA"
     gene            complement(8518..9510)
                     /locus_tag="VFK00_06865"
     CDS             complement(8518..9510)
                     /locus_tag="VFK00_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7592706.1"
                     /translation="MEDFSAIASFVRVVEARSFRAAAAQLNMTPSGVSRAVSRLEEQI
                     GVRLLFRSTRALRLTDDGESFYQRCKDILADLGDAVEALAYARTKPQGKLRVAVNVSI
                     GRAALIPNLADFEERYPDVRLELAMSDRNIGLIEEGIDCAIRMGELEDSNLIARKLGY
                     FSNVLCASPEYLARHGTPTSIEDLKQHKCVNYVYPTNGRTYQWQFDSPDGRIALDVDA
                     HLLINDGESVIQAAMAGLGIIQVPHWLAAGPIGKGKLEVIMQDTISTGSPVWIVYPQK
                     KHLSARVHAFMDWVQELFSRNNVPACRLMPGAKSACVVQEDAQPQYRSALVAET"
     gene            9657..10943
                     /locus_tag="VFK00_06870"
     CDS             9657..10943
                     /locus_tag="VFK00_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7592707.1"
                     /translation="MMKKKFVLIGLVVVASVVSWVVLDHRSGHAQAAPAGPPQVTVAQ
                     ALERPVSASAEFTGRLEPVDSVDVRPRVGGYIDAVGFDEGALVHKGDVLFKIDPRPYQ
                     AEVDRLVARRDQARAALKLARDNAGRAQRLLAKHAIAKAEADRQATAQRSAQAALSAA
                     DAELSAARLNLGFTTVRAPIDGRVSNAHVTPGNLVTSSDVLTSIVSVNPVYAYFNVDE
                     HTWLKLDHLRAAARDEGREPRIAVEMGLSDEQGYPHEGRLDFVDNQLHTDSGTMRLRA
                     VFDNADGLFTPGLYAHVRVQMGQPEPRILIDDRAVGTDLANQFVYVVDGEQKVQYRKV
                     STGPLFHGLRVIDSGLKPGARVVVNGLQRVRPGVQVNAEKVAMDYRLDAEDKALVEAA
                     GEQSRQDKRTAQTNRHDANASPIASLSPRERVPAGG"
BASE COUNT         1969 a         3792 c         3796 g         1622 t
ORIGIN      
        1 ggcggacgcg gccaaccagc aggccgaaca gatcacctcg gcctcggcca agatcaacga
       61 gatggcggtg tccatcgacc aggtgtcgcg caactccgcc gactcggcgg aagtggcgca
      121 gcgctcggtg cagatcgcat ccaacggcgc gcagatcgtg cgccagacga tcagcggcat
      181 ggactcgatc cgcgagcaga tccaggaaac gtcgaagcgc atcaagcgcc ttggcgagtc
      241 ctcgcaggaa atcggctcga tcgtcgaact catcaacgac atcgccgagc agaccaacat
      301 cctggccttg aacgcggcca tccaggcggc ttcggccggc gaggcgggcc gcggcttcgc
      361 ggtagtcgcg gacgaagtgc agcgactggc cgaacgcgcg accggcgcga ccaagcgaat
      421 cgaaacgctg gtccagacca ttcagtccga taccgtcgag gcggtcagct ccatggagca
      481 gacgacgaca caggtggtga atggtgcgcg gctcgccgag gacgccggca ccgcactggg
      541 cgagatcgaa aaggtgtcga ccaacctcgc cgggttgatc cagggcatct ccagcgccgc
      601 ccgccagcag tcggcggccg cgaccaacat ctcgtcgacg atgagttcga tccagcagat
      661 caccgcgcaa acttcggtgg gcgccaacca gaccgcggag tcgatcggaa acctcgcgca
      721 tctcgcgcaa gagctccgcc gctcggtggc cgacttcaag ctgccggcgt aagcggcggt
      781 tcgttgccat gaccgcaggc gatgacatga tgtccgcggc gaccggcgag gccggcgttg
      841 ccgctgctgc gcccgaagag gtggcggagc aggcggtggg cgtgagcgtg cggaaacagg
      901 cgatgcgact cgaagagcaa gccgaatttt cgaccctgag ctggatcaag ccccagatcg
      961 accagctcct gtcggaggcg cgccagtcgc tcgagaccta cgccgagaat cccgccgacc
     1021 ggcaggcgat gcagatctgc agcacgctgc tgcaccaggt gctcggcacg ctgcggatgg
     1081 tcgagctgta cggtgccgcc gaactcgcgc acgagatgga atgccttgcc caggcgctga
     1141 tcgaggacaa ggtcgacgac cgcgacgagg cattcggcgc gctgatgcgg agcctcgtgc
     1201 agcttccgga ttacctcgac ctgatcgaga gcggccacaa ggacatcccg atcgtcctgt
     1261 tgccgttgct caacgaattg cgtgaggcgc gcggctgcga agcggccgac gcgcgcgtgc
     1321 tgttccaccc cgatctggac cggccgctgc cgccggacgc cgcgggcgcg cgtgcgccgt
     1381 tcccgtttgc cgacctgcgc caacgcgcca cccggatccg ggcgcgcttc caggcgcagt
     1441 tgctgccctg gacccaaggc aaggcgccgg acttcgcggc catgcacgaa tgcatggacg
     1501 aacttcgcgc cttgtgccac gccgagccgg cccgccgcct gtggtgggtt gccggtggcg
     1561 tgctggaagc tttgcagcag ggccggctcg accaccatct cgccagcctg cgccagcagt
     1621 tcgggcacct cgaccgcacc atccaccgga tgcaggacca gggcgaggaa gtctgcggcg
     1681 acgaggatgc gcgcgaactc acccgcgacc tgctgttcca tgtggtgcag gccggtcccg
     1741 gcgccgggcg cagcgaagcc attgccgaat gcttcgcgct cgatcgcatg gtgccatccg
     1801 aagccgaact cgagcgcgcc cgtgcggcga tgtccggccg caaccgcgcg ttgctcgaca
     1861 cggtggccaa ggccgtccgc gaggacctgc tgcgcgtcaa ggagggcctg gacatctatc
     1921 tgcggcgtga cgaccgcgag ccggaccagc tcgcgccgca ggtggaaacg ctgcaccggg
     1981 tgtgcgaaac cctggccgtg ctggggttgg acacccccgc ccggatggtc cgcgaccagt
     2041 gcaacctcgt cgcgcagatc aacgacggcg agcgcgaagc gcgccccgag acgatcctcg
     2101 acatcgcgag cgcgctgctc tacgtcgacg cttccctcga cgaacacatc gaaaacctcg
     2161 gtggtcccgg cgggtccgac gaaacgctgc ccttcagcga gggccgcaag atcatgcggg
     2221 cgctgatgca cgaagccggc gccaacctcg gcggcgtcaa gggtgcgctg acggcgttca
     2281 tcgaatcgtc gtggtcgcac gccgaactcg ccgacgtccc gaagctgctc gccgaagtga
     2341 ccggcgcgct ccgcatcctg aacctggatg cccccgccga cgtcgtcgag ggcatcgacc
     2401 gctacatcgg caacgaattg atcgcggacc accgcgtccc cacggtcgat gaaatggaca
     2461 cgctcgccga cgccatggcg agcgtcgagt actacctcga aatcggtcgt gacgcgagcg
     2521 ccggggacaa ccggatcctc gatgcggcac gccgcagcct ggaagcgctg cactactggc
     2581 cgctgccgga gcgccgcgtg ccggcctatg cggatgccga atccggcgat gaagcggccc
     2641 ccgcgcgcgc tgcgccgccc gcatccggtc ccgtcaccgt ggggcgcggc ctgccgtcgc
     2701 ccgtcgacat tccggtcgcc gctgcgcaag ccaccgatgc cgggccttcg gccgccgaag
     2761 ccgtgccggg cgtcgggccc ggacaatgga tcgaagtcga agaagatgtc ctcgagccga
     2821 tcgccagcga aggcgtgtcc gtcgagaccg ccttccagga agccgccggc atcgacgagg
     2881 aaatccgcga agtcttcatc gaggaagtcg gcgaggaaat cgccaacatc aacgtcaacc
     2941 tgccggcgtg gaaagccgat ccggaagacc tcgacacgct caagaacgtg cggcgttcct
     3001 tccacacgct gaagggctcg ggccggctgg tcggcgccgt cgcactgggc gagttcagct
     3061 ggaagatcga gaacatgctc aaccgcgtgc tcgatcgcac gattctgccg gacggcaacg
     3121 tcctcgaagt ggtcgaagcg gcggttgcga ccttgccgaa gttgcacgcg ggcttgcgcg
     3181 gcggcgcgat agaactcaac ccgccgctcg acgcgatcac gcagacggcg gacaagctcg
     3241 ccgcgggcga gccggcccgg atcgccgatt cgatgggcgg ctatcgcaag gtcctgcgca
     3301 aggtgcgtcg ctgggtgccc gcggacgagg gcgaggcgcc gccggttccg ctgtccgacc
     3361 aggtcgttac ctcgccggag tcgggcctgc cggtggtcga cccgatcctg ctggacgtgc
     3421 tgcggaccga ggtgggcctg catctggaac gcatgcgcgg atacatcggc cgcaacgcga
     3481 accatgacgt ggcggttgac gacgaactgg tgcgctcggt ccataccctg cacggcgccg
     3541 tggccatggt cggcatcgaa tcgctggcgc gcatgctggg gccgacggaa gtctgggttc
     3601 ggcgcgtgcg cagcgccggc gccccgctcg atcgcgaggg ctgcgatgcg ttgcgcgatg
     3661 cggcgtcggt gaccgaacac gtgatggcgg agttcgatgc accggtgccg gacctgcccg
     3721 acatcacggc actcgccgag cggatggaag ccttgcgcga ccgctggccg gaagccgccc
     3781 ccctgggggc gccgcgcgcg gccatggccg atgccgcgga agccatcgag ctgtccgagg
     3841 aggatctgga cggagccctc gccataggac tcgaagccgc ctccatgctt ccgaccgaag
     3901 acgacgagga cgtgctggcc gcgcgcgagg aatccgggcg ccgcgccgag gacaaggcgg
     3961 aagccgagcg ccacgccgcc gagcaggcgg acgccgaacg tgtcgcggcg gacaaggcgg
     4021 aagccgagcg gcttgccgcc gaacaatccg aacaggggcg cgctgcggcc gagcaggtcg
     4081 aagccgagcg cagcgcggcc gagcaagccg aacgggaccg cattgccgcg gaacacgccg
     4141 aacaggaacg cgtcgccgcc gagcaagccg aacaggaacg catcgccgcc gagcgggcgg
     4201 aacaggaacg catcgctgcc gagaaggccg aagccgaacg cgtcgccgca gagcaagccg
     4261 agcgggaacg catcgcggcc gaacaggccg aacaggaacg tatcgccgcc gagcaagccg
     4321 aacaggaacg cattgccgcc gaaaacgcgg aagtcgaacg catcgccgcg gaacaggccg
     4381 agcaggagcg aatcgccgca gagcaggccg aagccgaacg actcgcggcg gaacaggccg
     4441 aacgggaacg catcgctgcc gagaaggccg aagccgagcg catcgccgcc gcccgcgaag
     4501 tcgccgcgcg cgaaatcgcc gctgccgcct tgccgccgtt cccggaagat ccccaaccgg
     4561 atgggccgct ggacatcccc gatctcgatc ccgagctcgt cagcctgttc accgaagaag
     4621 cgggcgaact gctcgattcc agcgacagcc aggtgaccac gctgcgccac gcgccggatg
     4681 acgtggaaag cgtacgcggg ctgcagcgca acctgcacac cctgaagggc ggcgcgcgcg
     4741 tggtgggcat cgccgcggtc ggcgatctag cgcacgcgat ggaaaccctg ctggaaagaa
     4801 cgggcgatcg ccagcgcgaa ctgacgccga tcgaagtcga ttcgctcgag cgcggcttcg
     4861 atcgtctgca cgacctggtc cagcgcctcg tccagggtcg cgcgctggcc gatcccgcca
     4921 acgcgatcga gcgcttcaac gcgctggccg aaggtcgccc ggtcgatggc tcggcccgcc
     4981 cggacgaggc ccccgcgccc gccaggccgg caccgccgcc tcgccccgcg ccgctgctgg
     5041 acgaaaccga aatccaggcg ccggccgaga tgatccgggt tcgctcggac ctcctggatt
     5101 cgctggtcaa cgccgcgggc gaagcctcaa tctaccgcgc acggctggaa cagcagatcg
     5161 gcaacttccg cttcaacctg gtcgagctcg accagaccgt cgcgcgcctg cgcgaacagt
     5221 tgcgcaagct cgagatcgaa accgaaaccc agatcctctc gcgctaccag cgcgagaacg
     5281 aagccggcga gggcgccacg ttcgacccgc tggagctcga ccgcttctcc aacctgcagc
     5341 agtattcgcg cgcgctcgcc gagtcggtgt cggaccttgc ctcgatcgag gacatcctcg
     5401 ccgaccagac gcgccagtcc gaaaccctgt tgctgcagca gtcgcgggtg aactcggatt
     5461 tgcaggacgg cctgatgcgc acgcgcatgg tgccgttcga gtcgatggtg ccgaacctgc
     5521 gccgcacgct gcgcggcgcc gccgacgagc tcggcaagcg cgcgcagttg cgggtggaag
     5581 gcgcgcaggg cgaaatggac cgcaacgtgc tcgagcgcat gaaggcgccg ttcgaacaca
     5641 tgctgcgcaa cgcgctcacc cacggcatcg aggatccggc gcaacgcgtc gcgatcggca
     5701 agccgatgga ggggactgtc accatccagg tcggccgcca gggcaccgaa gtgctggtgc
     5761 gggtttccga cgatggcgcc ggcttcgacc gcgacgcgat ccgtgccaag gccatcgaac
     5821 gcgggctgct gaagcccgag gtgccggtgt ccgatgccga gctgctcaac ttcgtgctgc
     5881 agacggggtt ttccacggcc agcgaaatca gccagctcgc cggccgcggc gtcggcatgg
     5941 acgtggtggc caaccagatc cggcaactgg gcggttcgct cacgattgaa tccgagcgcg
     6001 gacgcggcac cgtcttcaac atccggttgc cattcacgct ggccgtcacg caggcgatca
     6061 ccgtgcgcgc ggccgatacc acgttcgcga tcccgatgac ttcggtccag gccgtgtcgc
     6121 gcatccgccc cgaggaactg gtggagcgca aggcaagcgg ccagatgacc gtgaaccacg
     6181 ttggcgagga atacacgttg cacgatcttt ccgaattgct gggcctcggg atctcgccga
     6241 tccccgacga gggcagcggc caattgccgc tgctgatggt gcgcgccggc gacctccgcg
     6301 ccgctgtccg catcgacacc gtgatcggtt cgcgcgaaat cgtggtcaaa ccggtcgccc
     6361 cgcaggtcag caacgttccg ggcatgttcg gggccaccat catgggcgac ggatcggtga
     6421 tgctgatcct cgacctggcg ccgctggtcc ggcacgtggt cgccaaccgc gcggccgcgc
     6481 tcgccatcgg cgaagccgac gccgtgccgc aggagcccgt cgtggaagcg ccgcgcagcc
     6541 gcgagggcgg cccgcggatg gtgatggtgg tcgacgattc gatcacgatg cgcaaggtca
     6601 cgacccgcgt cctcgagcgc gagcagctcg aggtcgtcac ggcgaaagac ggcgtcgacg
     6661 cgctcgaaaa gctgcaggag cgcgtgcccg acgcgatgtt gctggacatc gagatgcccc
     6721 ggatggacgg ctacgagctc gcgacgcaca tgaagaacga cgcgcgtttc cgccacgtgc
     6781 ccatcatcat gatcacctcg cgcaccggcg aaaagcaccg ccagcgtgcg ctggagatcg
     6841 gcgtcgagcg gtacctcggc aagccctaca gcgaagccga cctgctgcac cacgtcaggg
     6901 aaatgctgga ggccggccgt gcctgaccat ctcatcccca ggctgccgcg gatcgccctg
     6961 gtgttcggtg accaagccgc cgccgcgcac ctgcgcgaag ccctgcaggc ccacgtcgaa
     7021 atcgcctacg cgagcgatgc cggcggcttc gacgccgcac ggatggccgc ggcgaacgtc
     7081 gacgccgcgc tggtgaatct cgacgatggc ggtggcctcg acacgttcga agccttgctg
     7141 gacgcggccg atgtcccggt cgtctacaac gatcccgaga tttcccgcga actggaaggc
     7201 tgggcgcgtg cgcgctggtt gcggcacctg ctggccaagc ttcggggcag taccgacgtg
     7261 gatccgcccc gccccggaca accggcggcc gggttcgcgg tcccttccgc tgccgcctcc
     7321 cacgattcac gggcgccgca ccacacgggc gtgttcgagc gcccattgtc gccacaggag
     7381 atcgaaacca tgactgccga cttcgtacag gaaagtgccg gcgtgccagc cggcgacatg
     7441 gggcagtccg ctgtcgcgga tgcgccggcc caaaccgcgg cgaccggcgc tctggcggat
     7501 gcggcattgg agccggtaca agcgcacggc gttgacgacc tcgacgtcga caccgaaacc
     7561 ttgtccgcca tgatcgacgc gcgcctcgcc gatgccgatt cccgttcacc ttcggattcg
     7621 ccggaagtgt ggcgactcgt tgaaggcggt acggcggcga acgcgatgcc tgatgcggga
     7681 gcggaaccgg gatccagcgt ggcgcctgtg gtcgccacca gcggggctgt cgaggattcc
     7741 gaggtgccgg tcagcctgcc ttcgctggat gactggcaac tggtcgatac cgacgccgcg
     7801 cctgcgccgg ccccgcgacg cgagcaacag aagaactccg acctggcgct cgcggagagc
     7861 ctggccgggc tgtcgctgga actcgagccc atggaaaccg tcaccacggt cgtccacgcc
     7921 gaaccgatcg agcgctggtt gcacgagagc gaaccgcgca agaccgcggc cagtgcccgc
     7981 agcgatgcgg aggcggaatc gatcggaggc aaggcgtgac caccccgagg ctgaggccgc
     8041 aggagattcg cggcgtcatg ctgccggtca ccggcggccg gctgctggtt cccaacacca
     8101 ccatggccga ggtcatcacc tacgcgcatc cggcggcagt cgaaggcgcg ccctcatggc
     8161 tgctcgggcg gctcacctgg cgcggctggg gactgccggt gctggcgttt tccgtgctcg
     8221 cgggcgtgtc gggcggcgaa tcgaccggca acgcgcgggt tgcgatcctg aaggccctga
     8281 acggccatcc gcgactgcct taccttggag tactcaccca gggcttcccg cgcctgacgc
     8341 tggtttcgga agagatgctg gttccggatc cggatgccgt ggacctgccg accggcgtcc
     8401 gcgaacaggt gctcgtccac gaggaaccgg cctggatccc ggatctcgcc acgctcgagg
     8461 acctggcggc gcaaacgttg actgcggctg cctgagggca gtgggcgcct gcgcccctca
     8521 ggtttctgcc accagggcgg atcgatactg gggctgcgcg tcctcctgaa ccacgcatgc
     8581 cgatttcgcg cccggcatca gccggcaagc cggcacgttg ttgcggctga agagttcctg
     8641 cacccagtcc atgaaggcgt gcacgcgggc cgacaggtgt tttttctgcg ggtacacgat
     8701 ccataccggc gagccggtcg aaatggtgtc ctgcatgatg acctcgagct tgcccttgcc
     8761 gatcggcccg gcggccagcc agtgcggcac ctggatgatc ccgaggccgg ccatcgcagc
     8821 ctggatcacc gattcgccgt cgttgatcag gaggtgcgcg tccacgtcca gcgcgatgcg
     8881 tccgtccggc gagtcgaact gccattgata cgtgcggccg ttggtcgggt acacgtagtt
     8941 gacgcacttg tgctgcttga ggtcctcgat ggatgtgggc gtgccgtgcc gggcgaggta
     9001 ttcgggtgaa gcgcacaaca cgttgctgaa gtaaccgagc ttgcgcgcga tgaggttgga
     9061 atcctccagc tcgcccatcc ggatcgcgca gtcgatgccc tcctcgatca ggccgatgtt
     9121 gcggtcgctc atggccaatt ccagccggac gtcgggataa cgctcctcga aatccgcgag
     9181 gttcgggatc aacgccgcgc gtccgatcga cacgttcacc gcaacccgca gcttgccctg
     9241 cggcttggtg cgcgcgtatg ccagcgcctc caccgcatcg ccgagatcgg ccaggatgtc
     9301 cttgcaacgc tggtagaacg attcgccgtc gtcggtgagc ctgagcgcgc gcgtgctgcg
     9361 aaacagcaac cgcactccga tttgctcttc cagcctcgaa accgcgcggc tgacccccga
     9421 cggggtcatg ttcaattgtg cggcggcggc cctgaaactc ctggcctcca ccacccggac
     9481 gaagctcgct attgctgaaa agtcttccat gatgatcatt atgtcgccat tcatgactgg
     9541 aagtcacgag tggaatgcct cgggaggggg ttaactgtac cgatcagtgt tcttatctct
     9601 ggcgctcgcg aagtgccggt gtcggcgcgc gattccgtca gagaggactg ctggtgatga
     9661 tgaagaagaa atttgtcctg atcggtttgg tggtggtggc cagcgtggtg agctgggtcg
     9721 tgctggacca tcgcagcggg catgcgcagg cggctccggc cggcccgccg caggtcacgg
     9781 tggcgcaggc gctggagcgc cccgtcagtg cgagcgcgga gttcactggc cggctcgagc
     9841 cggtagacag cgtcgacgtg cgcccgcggg tgggtggcta catcgacgcg gtgggcttcg
     9901 acgaaggcgc gctggtgcac aagggcgacg tgctgttcaa gatcgatccg cgcccatacc
     9961 aggccgaggt cgatcgcctc gtggccaggc gcgaccaggc ccgggcggcg ctgaagctcg
    10021 cccgggacaa tgccggtcgc gcccagcgcc tcctggccaa acatgcgatt gccaaggccg
    10081 aagcggatcg ccaggccacc gcccagcgaa gcgcgcaagc cgcgctgtcc gcggcagatg
    10141 ccgaactgtc ggcggcacgt ttgaacctgg gcttcacgac cgtgcgcgcg cccatcgacg
    10201 gacgcgtcag caatgcgcac gtgactccgg gcaacctcgt caccagcagc gacgtgctga
    10261 ccagcatcgt ctcggtgaat ccggtgtacg cctatttcaa cgtggacgaa cacacctggc
    10321 tgaagctgga ccacctgcgc gcggctgcgc gggatgaggg ccgcgagccc cgcatcgctg
    10381 tcgaaatggg gctgtcggac gaacagggct atccgcacga aggacggttg gacttcgtcg
    10441 acaaccagtt gcacaccgat tccgggacga tgcgcctgcg tgcggtgttc gacaacgcag
    10501 acggcttgtt cacgcccggc ctctacgccc acgtgcgcgt gcagatgggg cagcccgagc
    10561 cgcggatcct gatcgacgac cgcgcggtcg gcaccgacct cgccaaccag ttcgtctacg
    10621 tggtggatgg cgagcagaag gtgcagtacc gcaaggtcag caccggcccg ctgttccacg
    10681 gcctgcgcgt gatcgacagc ggcctgaagc cgggtgcccg cgtcgtggtg aacgggctgc
    10741 agcgcgtacg cccgggcgtc caggtgaatg ccgaaaaggt ggcgatggac tatcgcctcg
    10801 acgccgaaga caaggcgctg gtcgaagccg ccggcgagca gtcgcggcaa gacaagcgca
    10861 ccgcgcagac caatcgccat gacgccaatg cgtcgcctat cgcatccctc tccccccggg
    10921 agagggtgcc ggcaggcggg tgagggtacg gccagggcga tgcgccgaag cttcgccgaa
    10981 ccctcatccg cccttcgggc accttctccc gaagggagaa ggaaatgccc ccgcaaggct
    11041 gaagaccagg aaaccccatg aagaacattg cccaattttt cgtcgaccgg ccgatcatgg
    11101 ccgcggtgct gtcgctgctg ttcctgatca cgggcggcat cgcggtgttc cagttgccga
    11161 tcagcgagta tccggaagt
//
LOCUS       DASSNH010000185        11191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_515794, whole genome shotgun sequence.
ACCESSION   DASSNH010000185 DASSNH010000000
VERSION     DASSNH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515794"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFK00_06875"
     CDS             complement(<1..942)
                     /locus_tag="VFK00_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HET7592708.1"
                     /translation="MKLAALTRIRIPVFSVTARLTAVIGMLLAMLVVGAWIGLNGMSR
                     GNHDVGRIYASGLVPLSVSDTVSRNTLRNFISLSEAAFRIKDKAIVEQKLHEVATREK
                     ATDALVKQLKGYSLTPAVGKQLQAFTRARSDVLSSIESVHDALASGDAGASELLYTGL
                     LPSVAIMQGEMDKLIKMQVDSGQRLHQEAAQRYQQIRTVTLMVLVVGIVLALLIAVLV
                     IRSVVVTLSRSVEVAHAIADGHLGHHIVTNGKGELGRLLAALRTMDERLCGIVGQVSR
                     GSDEVSVAAQQISRGNDDLSQRTQEQASSLEETASSME"
     gene            complement(965..1465)
                     /locus_tag="VFK00_06880"
     CDS             complement(965..1465)
                     /locus_tag="VFK00_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7592709.1"
                     /translation="MSQSEQPARAVQQLTFTLAGEEYGIDILSVREIRAWSRVTRIPQ
                     APEYVLGVLNLRGTVVPVMDMRLRFGLPREGYGSDTVVVVVAVGERLFGIVADAVADV
                     FDAPADTIKPVPDLGAVVDTRFLKGLAGDGERMIMLLDVERLMRPGEAEALDALLAPA
                     DAEAVA"
     gene            complement(1583..3568)
                     /locus_tag="VFK00_06885"
     CDS             complement(1583..3568)
                     /locus_tag="VFK00_06885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET7592710.1"
                     /translation="MATVDLSQFHQTFFEESLENLAVMEDALLALEHGVQEPGADDRE
                     LVHTIFRAAHSIKGGAATFGFSDVAAFTHVLESLLDEVRAGRRAADGGIIESLLQAVD
                     CLAGMLARSRDGGAVADAMSESLRERFTRMLGKPVEATAAKAAPALAAAGWRIRFRPL
                     PHMLRTGNDPLRMFRELASHGALVVKQVHFLEQPRPAFEDLDPTAATLGWDLELHGDI
                     PRSQVDAVFEWVDGDCELDIEVLHDRRTRCQAPDAAAQAATAPAPEPGVHADAGSIRV
                     SIAKVDALINMVGELVITQSMLGDIGEHFSLDRLARLREGLAQLDRTTRELQDSVMRI
                     RMLPIAAVFHRFPRMVRDLEHRLGKRVKLELRGESTEVDKTVLERIGDPLVHLIRNAV
                     DHGFEKPEARVAAGKPETGMLRLEARHEGGNVVVEVSDDGGGLRRAPILAKAEQSGLV
                     RPGQEIDDEELAELIFQPGFSTHAEATDVSGRGVGMDVVRRNVRDLGGSVSVRSREGA
                     GSTFTIVLPLTLAIIEGLVTGVGGERYIVPLVSIVESLQLRTDGVRHVGGGGELFRFR
                     DDWLPLVRLHDVFGCTGAVTEVEQGMVMVVEAGGRRVGLLVDALMGQQQAVIKSLEAH
                     YRRVEGISGATIASDGGVALIVDVGGIVGLAGRRKAA"
     gene            complement(3586..3948)
                     /locus_tag="VFK00_06890"
     CDS             complement(3586..3948)
                     /locus_tag="VFK00_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592711.1"
                     /translation="MPKILAVDDSVSMRNMVSFTLRGAGHEVCEAADGEAALAAARAE
                     RFDLVLADINMPGMDGITLTRELRALPSFAGVPILILTTESDRDKKLEGKAAGATGWL
                     VKPFDPDQLLATVARVAG"
     gene            complement(3970..4293)
                     /locus_tag="VFK00_06895"
     CDS             complement(3970..4293)
                     /locus_tag="VFK00_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7592712.1"
                     /translation="MAGKRKRSRPGECRVALAADLVVAGAGDMHARLRDALAQTGPVV
                     LDAAGVARLDTSGLQLLEAFIHAREAAAAPWRWDNVGEVLREAAARLGLQQMLKLPEA
                     VPASD"
     gene            4507..5679
                     /locus_tag="VFK00_06900"
     CDS             4507..5679
                     /locus_tag="VFK00_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET7592713.1"
                     /translation="MQPPQLAALDWAAAAAGTLARPWKVGSVVSGRVLEYLPAMRLVL
                     QINGLKVEAEPPATWPVPRQFQAKVLSAGPQPVLELLDPPEASQSSPAVTALRARLPS
                     QGGLQPLLADLHALARLPGARALPEPARAALARLEGSIADRHDVLDPDVLRDTLQRGG
                     TQLEHNLLQRARAPERMPATQVDYDIKAALQRLTQALQSLPKTGAPVPEAPEPPPPPL
                     LQLPLQPQPRLPAMPAADALVLAGNLLKHAEAALSRIEIMQLDAHPSASPQGCMIEVP
                     IRGDDGFDVLQVRIEQDGTGQGVQAAEEPRWTLGFTLEPPALGPVHGTVRLRGAEVNV
                     DLWAQHDRAVQALEEQTSVLSQLLEGSGLRLAQLRIRQGSPMRHTGLGHSLLEARA"
     gene            5676..5942
                     /locus_tag="VFK00_06905"
     CDS             5676..5942
                     /locus_tag="VFK00_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HET7592714.1"
                     /translation="MNAAPRSVTLRFRDHGAPAEGTLRPDLGADEVERLLEEAERLGL
                     PRHPDPSTAALLAAVRLRTDVPQELYAAFAAVLSRIYAATEKLR"
     gene            complement(6096..6455)
                     /locus_tag="VFK00_06910"
     CDS             complement(6096..6455)
                     /locus_tag="VFK00_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592715.1"
                     /translation="MKKIIAAGALLLSPVLAHAACSPADFKVAAFDVMSGDAAHPVLE
                     MPGKLVNHCDEPAAAQLQIEARSDDGSVVQKRKFWPAGTANIAPGASVKFDAGRMFSY
                     KASMKTYSVSIVSVRSW"
     gene            complement(6497..7768)
                     /locus_tag="VFK00_06915"
     CDS             complement(6497..7768)
                     /locus_tag="VFK00_06915"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET7592716.1"
                     /translation="MSLSTAPTQMDHESGIEVEVLPFVRVPILDRNMQLVAYDIRHQS
                     DEGGAKLAPATLQLFGLVSLLDQLTGRQLSFFAVDEELLRGHADLLRFDGRVGPRVDV
                     KLAASDDVYAVLRGMAARGVPVMIDDLVWSDALPEASVQRFEELAQLASHVSIDPARH
                     DEATLLRTIGRLRAADTVVVASCLSTREVHGTCMDLGFDAFEGSYLFEPPAKSAIDSL
                     KPSRLNVLRLLAAVQDPENGPNELEALIRNDAVLSYKLLACVNSAYFGVPRELKSLQQ
                     AAVYFGVNHIRNWVYAMAVGDLDDTSPEVLKQALLRARMSELLGRQLPPERREMAFTM
                     GLFSLLDTIMGVPMERVLADVPVAAEVREALVGGSGPLAGVLQQIRAWESGHVVRATS
                     SGGVVVDLAEAYLDSLEWAEHVYSHAQRSAA"
     gene            complement(7784..8221)
                     /gene="flgN"
                     /locus_tag="VFK00_06920"
     CDS             complement(7784..8221)
                     /gene="flgN"
                     /locus_tag="VFK00_06920"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HET7592717.1"
                     /translation="MPLAPEFDDARRAVYAEMASSVDALRGTLDAEREALDQLDPVAL
                     DAATSAKARLLLRLEALEAERRQLDDFAAPRESSGTWQRIRSQLEDCRRMNETNGSIV
                     EHQLRGVRRALGILRGAGEAPPLLYGPGGHADAPVLPHSLSKA"
     gene            complement(8223..8534)
                     /gene="flgM"
                     /locus_tag="VFK00_06925"
     CDS             complement(8223..8534)
                     /gene="flgM"
                     /locus_tag="VFK00_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HET7592718.1"
                     /translation="MNNTIHPGGPTAPQTAATGAKPAVPGDVDSAASRATGSVPAQGG
                     DHVQLTESARALGAATRGTDAPVDTKHVERIRQAIADGTYKVDPQRVAERLIALEKQI
                     G"
     gene            complement(8635..9327)
                     /gene="flgA"
                     /locus_tag="VFK00_06930"
     CDS             complement(8635..9327)
                     /gene="flgA"
                     /locus_tag="VFK00_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HET7592719.1"
                     /translation="MKRMMAAATLLIAMAGTVHAAAVQSLPELQATAVAAVRQAAPAD
                     ARIVAEAEPLDSRLRLPACSGELQAEAPDLARGSSRVSVAVSCAVGPSWTVRVPVHVQ
                     IFRKVLVSSRALARGDVINAGDIRAEEQDVARLGYGYLAESAQVYGRHLRRPVGADTV
                     LTPGMLTAREVVQRGQQVSLVARMDGILVRGGGVALQGGDRGDLVRVRSLSCDCVVQG
                     TVRDPGVVETLP"
     gene            complement(9324..10622)
                     /locus_tag="VFK00_06935"
     CDS             complement(9324..10622)
                     /locus_tag="VFK00_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7592720.1"
                     /translation="MSSSHVEALAQALAAGDRQCARELAQRHPAAARALAPLLSAGSQ
                     REQQEAWLRALEQQGLALSGTRRLGQRLREMPDQLAGARQHLDGVARDGDGITSLARQ
                     AAASLQQGAEKIGAGKQDLAELDGQLSLLRGTLCGMTRTHERFTGYFEEIARLTAVVQ
                     DIAHQTNLVALNAAIEAARAGESGRGFAVVADEVKQLAEKTAAATAEIETVTEAVDGF
                     SGTLEETVGGGLARLDGAGKGVEALRGTLDATDAALGDARSRLQALQGTGDGLSGRAR
                     SGGALLAKLQRSGEECSRHLDAVEHAAVAAQQSALLGVPHLAATDGAGAIRMVRESCA
                     ALRHGLDLLARTPARLDRRWMDGASLRAWMAQLRDARPALPALEAVQESLRQFEQRRG
                     QLDMALEEGESARCDELVPAMQADLDAIQKQLGVLAEDDA"
BASE COUNT         1702 a         4047 c         3716 g         1726 t
ORIGIN      
        1 ctccatcgac gacgccgttt cctcgaggct cgaggcctgt tcctgggtgc gctggctcag
       61 gtcgtcgttg ccgcgcgaaa tctgctgcgc ggcgacgctg acctcgtccg atccgcggct
      121 cacctggccg acgatgccgc agaggcgctc gtccatcgtg cgcagcgcgg ccagcaaccg
      181 gccgagttcg cccttgccgt tggtgacgat gtggtgcccg agatgaccgt cggcgatcgc
      241 gtgcgccacc tcgacggatc ggctcaacgt cacgacgacc gagcggatca ccagcaccgc
      301 gatcagcagc gccagcacga tgccgaccac cagcaccatc agtgtcaccg tgcggatctg
      361 ctggtagcgc tgggccgctt cctggtgcag gcgctggccg ctgtctacct gcatcttgat
      421 cagcttgtcc atttcgccct gcatgatcgc gaccgacggc agcaggccgg tgtacagcag
      481 ctcgctcgca cctgcatcgc cggaggccag cgcgtcatgg acgctttcga tcgacgaaag
      541 cacatccgac cgcgcgcggg tgaacgcctg caactgcttg ccgacggccg gcgtgagcga
      601 gtagcccttg agttgcttga ccagtgcgtc ggtggccttc tcgcgcgtgg cgacctcgtg
      661 gagcttctgc tcgacgatgg ccttgtcctt gatccggaac gccgcttcgc tgaggctgat
      721 gaaattgcgc agggtgttgc gcgacaccgt gtcggaaacc gacaacggca ccagcccgct
      781 ggcgtagatg cggccgacgt cgtggttgcc ccgcgacatt ccgttcaatc cgatccacgc
      841 gcccaccacc agcatcgcca gcaacatgcc gatgacggcg gtgaggcggg ccgtgacgct
      901 gaacacggga atgcggattc gggtcaaggc tgcgagtttc atcttcatcg ttcccccgga
      961 atcgtcaggc caccgcttcc gcatccgcgg gagccagcag tgcatcgagc gcctcggctt
     1021 cgccggggcg catcaggcgt tccacatcga gcagcatgat catgcgctcg ccgtcaccgg
     1081 ccagtccctt caggaaccgg gtatccacca cggcccccag gtcgggaacc ggcttgatcg
     1141 tgtccgcggg cgcgtcgaag acgtcggcca cggcgtcggc gacgatcccg aacaggcgct
     1201 cgccgaccgc caccaccacc acgaccgtgt cggacccata gccttctcgc ggcaggccga
     1261 agcgcagccg catgtccatg accggcacca cggtgcccct gagattgagc acgccgagca
     1321 cgtactccgg ggcctgcgga atgcgggtga cccgcgacca ggcacggatc tcgcgcaccg
     1381 acaggatgtc gatgccgtat tcctcgccgg ccagcgtgaa agtgagttgc tggactgccc
     1441 tggccggttg ttcgctctgg gacatcgtgc tgctacctcc gggtggcgcc ggcgcgccgt
     1501 tgttctcctg ctttatcgac cgcagggacg gatccttgag gtgacgacgg ctcaaggagc
     1561 gcagcgccac atatgcggcg cgctacgccg ccttgcggcg cccggccagg ccgacgatgc
     1621 ccccgacatc cacgatcagg gccacgccgc cgtcgctggc gatcgtggcg ccggaaatac
     1681 cctcgacccg ccggtaatgc gcctccagcg acttgatcac cgcctgctgc tggcccatca
     1741 gcgcatccac cagcagcccg acgcgacgtc cgccggcctc gacgaccatc accatcccct
     1801 gctccacctc ggtcaccgca ccggtgcagc cgaacacgtc atggagccgg accagcggca
     1861 accagtcgtc ccggaagcgg aacaactcgc cgccaccgcc gacgtggcgc acgccatcgg
     1921 tccgcagctg cagggattcg acgatggaaa ccagcggcac gatgtagcgc tcgccgccga
     1981 ccccggtgac cagaccttcg atgatcgcga gggtgagcgg caacacgatc gtgaaggtgc
     2041 tgcccgcgcc ttcgcggctg cgaacgctca cactgccgcc gaggtcgcgg acgttgcggc
     2101 gcaccacgtc catgccgacg ccgcgcccgg acacgtcggt ggcttcggca tgggtggaaa
     2161 atccgggctg gaagatcagt tcggccagtt cctcgtcgtc gatttcctgg ccgggccgca
     2221 ccagcccact ctgctcggcc ttggcgagga tcggggcgcg ccggaggccg ccgccgtcgt
     2281 cgctgacttc gaccaccacg ttgccgcctt cgtggcgggc ttcgaggcgc agcatcccgg
     2341 tctccggctt gcccgcggcc acgcgcgcct cgggcttctc gaagccatgg tccacggcgt
     2401 tgcggatcag gtgcaccagc ggatcgccga tccgctcgag cacggtcttg tcgacttcgg
     2461 tggactcgcc gcgcagctcc agcttgacgc gcttgccgag gcggtgctcg aggtcgcgca
     2521 ccatccgcgg gaaacgatgg aacaccgccg cgatcggcag catccggatc cgcatcacgc
     2581 tgtcctggag ttcgcgggtg gtgcgatcga gctgcgccag cccctcgcgc aggcgcgcca
     2641 ggcgatccag gctgaaatgt tcaccgatgt cgcccagcat cgattgcgtg atcaccagct
     2701 cgccgaccat gttgatcagc gcatcgacct tggcgatcga cacacggatc gaaccggcat
     2761 cggcatgcac gcccggctcc ggcgcgggcg ccgttgcggc ctgggccgcg gcgtccggcg
     2821 cctggcaccg ggtacggcgg tcgtgcagca cctcgatgtc cagttcgcag tcgccgtcga
     2881 cccactcgaa gacggcgtcc acctggctgc gcgggatgtc gccgtgcagt tcgaggtccc
     2941 agcctagcgt cgcggcggtg gggtccaggt cctcgaacgc ggggcgcggc tgctcgagga
     3001 agtgcacctg cttcaccacc agcgcgccgt ggctcgccag ttcgcggaac atccgcagcg
     3061 gatcgttgcc ggtccgcagc atgtgcggca gcggccggaa gcggatccgc cagccggcgg
     3121 cggccaacgc cggcgccgcc ttcgcggccg tggcttcgac cggcttgccg agcatccgcg
     3181 tgaaacgttc gcgcagcgat tcactcatgg cgtcggccac cgcaccgccg tcgcgcgagc
     3241 gcgccagcat gccggccagg cagtcgaccg cctgcagcag cgactcgatg atgccgccgt
     3301 cggccgcgcg ccggccggcg cgcacctcgt cgagcagcga ctcgagcaca tgggtgaacg
     3361 cggcgacatc cgagaacccg aaggtggcgg caccaccctt gatggagtgc gccgcgcgga
     3421 agatcgtgtg gaccagctcg cggtcgtcgg cgcccggctc ctggacacca tgttccagcg
     3481 ccagcaacgc gtcttccatc acggccaggt tctcgaggct ctcctcgaaa aacgtctggt
     3541 ggaactggct gagatcgacc gtcgccattc tgggccctgc cctgctcagc ccgccacgcg
     3601 ggcgaccgtg gccagcaact ggtcgggatc gaagggcttc accagccagc cggtggcgcc
     3661 ggcggccttg ccttccagct tcttgtcccg gtcggattcg gtggtcagga tcaggatcgg
     3721 cacccccgcg aacgacggca gcgcacgcag ctcgcgggtc agggtgatgc cgtccatgcc
     3781 cggcatgttg atgtccgcca acacgaggtc gaagcgctcg gcccgcgccg cggccagggc
     3841 cgcttcgccg tccgccgcct cgcacacctc gtggccggca ccccgcaacg tgaaggacac
     3901 catgttgcgc atcgaaacag aatcatccac cgcgagaatc ttgggcattt cgaaaaacct
     3961 cggatcgtct caatcgctcg ccggaacggc ttccggcaat ttcagcattt gttgcaggcc
     4021 aagccgcgcg gcagcttcgc gcagcacctc gccgacgttg tcccaccgcc agggtgcggc
     4081 ggcggcctcg cgcgcgtgga tgaaagcctc gaggagttgc agcccgctgg tgtcgagccg
     4141 cgccaccccg gccgcgtcga gcaccacggg cccggtctgc gcgagcgcgt cgcgcaaccg
     4201 cgcatgcatg tccccggcac ccgcgacgac caggtccgcg gccagtgcga cccggcattc
     4261 gccgggccgg ctccgttttc gcttgcccgc catgcgacct ccgcatacga ctaccaacgc
     4321 tgtagcggcc cgcctcgacg caactttagg gaacaccggg cggggcgggc attgcccgcc
     4381 cacccaaggt ggcgtggcgg accgtgtcca ccctaccgcg agtgcccctc ggcgttcttg
     4441 ctcaagttca cacaagtcct gccgataccg accgcatacc tgaatgcatg gagctggcgc
     4501 ttgatcgtgc aaccacccca actggcggcc ctcgactggg cggcagccgc agcgggcacg
     4561 ctggccaggc cgtggaaggt tggctcggtc gtgtccggcc gcgtgctgga atacctgccc
     4621 gcgatgcgac tggtgctgca gatcaacggc ctgaaagtcg aggccgagcc gcctgccacc
     4681 tggccggtgc cgcgccagtt ccaggcaaag gtcctgtcgg ccgggccaca gccggtactg
     4741 gaactgctgg acccgcctga agcgagccag agctcgccgg ccgtgaccgc gctgcgtgcg
     4801 cgcctgccga gccagggcgg cctgcaaccg ttgctcgccg acctgcatgc actcgcgcgg
     4861 ctgcccggcg cccgcgcgct gccggaaccg gcgcgcgcgg cgctggcaag gctcgaaggc
     4921 tccatcgccg accgccacga cgtgctcgat cccgacgtgc tgcgcgatac gctgcagcgt
     4981 ggcggcacgc aactggaaca caacctgctg caacgcgccc gcgcacccga gcgcatgccg
     5041 gccacgcagg tcgactacga catcaaggcg gcgctgcagc gcctcacgca ggccctgcag
     5101 tcgctgccga aaaccggtgc gccggtgccc gaagccccgg agccgccacc accgccgctc
     5161 ctgcagttgc cgctgcaacc gcagccgcgc ctgccggcaa tgccggctgc cgatgcgctg
     5221 gtgctggcag gcaacctgct gaagcatgcg gaggccgcat tgtcgcggat cgaaatcatg
     5281 caactcgacg cccatccatc ggcgtccccg cagggctgca tgatcgaggt tccgatccgc
     5341 ggcgacgacg gcttcgacgt gctgcaggtc cggatcgagc aggacggcac gggccagggc
     5401 gtccaggccg cggaagagcc ccggtggacc ctgggcttca ccctggagcc gcccgcgctc
     5461 gggccggtcc acggtacggt gcggctgcgc ggcgccgaag tgaacgtcga cctgtgggcg
     5521 cagcatgacc gcgccgtgca ggcactggag gaacaaacct cggtgctgtc gcaactgctg
     5581 gaaggctccg ggctgcggct tgcgcagctg cgcatacgcc agggctcgcc catgcgccac
     5641 accgggctcg gccattcgct gctggaggcc agggcatgaa cgccgcaccg cgctcggtca
     5701 cgctccgctt ccgcgaccac ggcgcgccgg ccgaaggcac cctgcggccc gacctcggtg
     5761 cggacgaggt cgaacgcctg ctcgaagagg cagaacgcct gggcttgccc cggcacccgg
     5821 acccgtcaac cgccgcgctg ctggcggcgg tgcggcttcg cacggacgtg ccccaggaac
     5881 tgtacgcggc attcgcggca gtcctgtcgc ggatctacgc cgccaccgaa aagctgcgct
     5941 gagggaattc gtagccggcc ggcatcgcaa ggcgttccgc tgctgcgcgc tcgaatctgc
     6001 ctgcgacgtt ggatgtcatt ggtgcatgat ggcgggcggc gggcgtgccc gccctgcgtc
     6061 gctgcctggt tccccgttcc ctgttccctg cttttctacc agctccgcac ggaaacgatg
     6121 cttaccgaat aggtcttcat ggaagccttg tagctgaaca tgcggccggc atcgaacttc
     6181 acgctggcgc cgggagcgat gttcgcggtg ccggccggcc agaacttgcg cttctgcacg
     6241 accgatccgt cgtccgacct cgcctcgatc tgcagctggg cggcggccgg ctcgtcgcaa
     6301 tggttgacca gcttgcccgg catctccagc accgggtgcg ccgcatcgcc cgacatcaca
     6361 tcgaaggcag cgaccttgaa atcggcgggc gaacaggcag cgtgcgcgag cacgggggag
     6421 agcagcaatg cgcccgcggc aatgatcttc ttcatgacgg cttttccagg tttgacaggc
     6481 tgcacgcgcg ggaccttcat gccgcgctgc gttgggcgtg gctgtagacg tgctcggccc
     6541 actccagcga atcaaggtac gcctcggcaa ggtcgaccac caccccgccg gagctcgtgg
     6601 cgcgcaccac gtggccgctc tcccacgcgc ggatctgctg cagcacgccg gccagcggac
     6661 cgcttccgcc gaccagggcc tcgcgcacct cggcggcgac cgggacgtcg gccagcacgc
     6721 gctccatggg cacgcccatg atggtgtcga gcagcgagaa caaacccatg gtgaacgcca
     6781 tctcgcggcg ttccggcggc agttggcgac ccagcagttc cgacatgcgc gcgcgcagca
     6841 gggcctgctt cagcacctcc ggggaggtgt cgtcgaggtc gccgacggcc atcgcgtaca
     6901 cccagttgcg gatgtggttg acgccgaaat agacggcggc ctgctgcagc gacttcagtt
     6961 cgcgcggcac gccgaagtag gcggagttga cgcaagcgag cagcttgtag ctgagcaccg
     7021 cgtcgttgcg gatcagtgct tccagttcgt tcggcccgtt ctcgggatcc tggaccgcgg
     7081 ccagcagccg caacacgttc aagcggctgg gcttcagcga atcgatcgcg ctcttcgccg
     7141 gcggctcgaa caggtacgaa ccctcgaacg cgtcgaagcc gaggtccatg caggtgccgt
     7201 gcacttcgcg ggtcgacagg cacgacgcca cgaccacggt gtccgcggcg cgcaggcggc
     7261 cgatcgtccg cagcagcgtg gcctcgtcgt ggcgggcggg atcgatgctg acgtggctgg
     7321 cgagctgcgc cagttcctcg aagcgctgca cggaggcttc cggcaacgca tcggaccaga
     7381 ccaggtcgtc gatcatcacc ggcacgccgc gcgccgccat gccacgcaac accgcgtaga
     7441 catcgtcgga ggcagcgagc ttcacgtcga cccgtgggcc cacccgcccg tcgaaccgca
     7501 acaggtcggc gtggccgcgc agcagttcct cgtccaccgc gaagaacgaa agctggcggc
     7561 cggtgagctg gtccagcagg gatacgaggc cgaacagttg cagcgtcgcc ggcgcgagtt
     7621 tcgcgccgcc ttcatccgat tgatggcgga tgtcgtaggc gacgagctgc atgttgcggt
     7681 ccaggatcgg cacgcgcacg aaggggagga cttccacctc gatgccggat tcgtggtcca
     7741 tctgggtggg ggcggtcgac agggacatgg atttcaactt cagtcaggct ttggaaagcg
     7801 aatggggaag taccggtgca tcggcgtggc cgccgggccc gtagaggagc ggtggcgcct
     7861 cgccggcgcc gcgcaggatg ccgagtgcac ggcgcacccc gcgtagctgg tgttcgacga
     7921 tgctgccgtt ggtctcgttc atgcgccggc agtcttcgag ctggctgcgg atccgctgcc
     7981 aggtgcccga cgattcgcgc ggcgcggcga aatcgtccag ttgccggcgc tccgcctcga
     8041 gcgcctccag ccggagcagc agccgcgcct tcgcggaggt cgccgcatcc agcgccaccg
     8101 ggtcgagctg gtccagtgcc tcgcgttcgg cgtcgagggt gccgcgcaac gcgtccaccg
     8161 acgaagccat ttccgcgtag acggcgcgac gggcgtcgtc gaactcgggt gcgagcggca
     8221 tgtcagccaa tttgtttctc cagggcgatc aggcgttcgg ccacgcgctg cgggtccacc
     8281 ttgtaggtgc cgtcggcgat cgcctgccgg atgcgctcca cgtgcttcgt gtcgacgggc
     8341 gcatcggtcc cgcgggtggc cgcgccgagg gcgcgtgccg attcggtgag ctgcacgtgg
     8401 tcgccgccct gcgccggaac gctgcccgtg gccctggagg ctgccgagtc gacgtcgcca
     8461 ggcacggcag gcttggcgcc ggttgccgcc gtttgcgggg ccgtcgggcc accgggatgg
     8521 atcgtgttgt tcatgggaat ctcccgccac tcctgccgat tggcctatgg atcggccgct
     8581 tttgacaaaa ctttagagtt gccccgcggg ccggctttcg cgccaccgcc gcgttcacgg
     8641 cagcgtctcc acgacgccgg gatcgcgaac ggtgccctgc accacgcagt cgcaactgag
     8701 gctgcgcacc cgtaccaggt cgccgcgatc gcccccctgc agcgcgacgc ccccgccccg
     8761 caccagtatt ccgtccatcc gcgccaccag gctcacctgc tggccgcgct gcaccacttc
     8821 gcgcgccgtc agcatgccgg gcgtaagcac ggtatccgcg cccacgggcc ggcgaagatg
     8881 gcgcccgtac acctgcgccg attcggccag gtagccgtaa cccagccgcg ccacgtcctg
     8941 ctcctcggca cggatgtcgc cggcgttgat gacgtcgccg cgggccagcg cgcggctgct
     9001 cacgagcacc ttgcggaaga tctgcacgtg caccggaacc cggacggtcc agcttgggcc
     9061 gaccgcgcac gacaccgcga cggatacccg cgaggaaccg cgggcgagat ccggagcctc
     9121 tgcctggagt tcaccggagc acgccggcag gcgcaggcgc gaatcgagcg gctcggcctc
     9181 cgcgacgatg cgcgcgtcgg caggcgccgc ctggcgcacc gccgcgacgg ccgtcgcctg
     9241 cagttccggc agcgactgca ccgcggccgc gtggacggtg cccgccatcg cgatcagcag
     9301 cgtcgccgcg gccatcatcc gcttcatgcg tcgtcctccg ccagcacgcc gagttgtttc
     9361 tggatcgcgt cgaggtcggc ttgcatcgcg ggcaccagct cgtcgcagcg cgcgctttcg
     9421 ccttcttcga gggccatgtc cagttgcccc cggcgttgtt cgaactggcg caggctttcc
     9481 tgcaccgcct ccagggccgg caaggcgggc cgggcgtcgc gcaattgcgc catccaggca
     9541 cgcagcgagg cgccatccat ccagcggcga tcgagccgcg ccggcgttcg cgccagcagg
     9601 tccagcccgt ggcgcagggc ggcgcaggat tcgcggacca tccggatggc gccggcgcca
     9661 tcggtggccg ccaggtgcgg aacacccagc aacgccgact gctgggccgc gacggcggcg
     9721 tgttccaccg cgtcgagatg gcgcgagcat tcctcgccgc tgcgttgcag tttcgccagc
     9781 aacgctccgc cactgcgcgc gcggccggac aggccatcgc ccgtcccttg cagcgcttgc
     9841 aggcgcgacc gggcgtcccc gagcgcggcg tcggtggcgt cgagcgtgcc gcgcagcgcc
     9901 tcgacgccct tgccggcacc gtcgagcctc gcgaggccgc caccgaccgt ttcttccagc
     9961 gtgccggaaa aaccgtcgac ggcttcggtc acggtttcga tctcggcggt ggctgctgcc
    10021 gtcttctcgg cgagttgctt gacctcatcg gcaaccaccg cgaaaccgcg cccggactcg
    10081 ccggcgcggg ccgcctcgat cgccgcattc agcgccacca ggttggtctg gtgcgcgatg
    10141 tcctggacca cggcggtcaa acgggcgatt tcctcgaagt agccggtgaa ccgttcatgg
    10201 gtacgggtca ttccgcagag tgttccgcgc agcaggctca actgtccgtc gagttcggca
    10261 aggtcctgct tccccgcgcc gatcttttcc gcgccctgct gcagcgacgc agccgcttgc
    10321 cgcgccagcg acgtgattcc gtcgccgtcc cgtgccacgc cgtcgagatg ttggcgcgcg
    10381 ccggccagtt ggtccggcat ttcgcgcagg cgctgcccca gccggcgggt gccggacaat
    10441 gcgagcccct gctgttccag cgcgcgcagc cacgcctctt gctgctcgcg ctgcgacccc
    10501 gcggacaaca aaggcgccag cgcgcgcgcg gcggccggat gccgctgcgc aagctcgcgg
    10561 gcgcactgcc gatcgcccgc ggccaaggcc tgggccagcg cctcgacatg actgctcgac
    10621 aagaatccca tcgctgaaag ttgctccgga aaccaaagtg gatcgtggtg cgcggcgcat
    10681 tgccactgga tcggcggata gccgcgcccc ttgactgaag caagccgcat gccaccggga
    10741 gcagggaaca gggaataggg aacggggagc agggaacggc gaccaaaccg gtcgtcccgt
    10801 cggtccatgc aacgcgcgcc ctcgccgcca ccgcgcgctc catcgaaacc cggcccccgg
    10861 tccctggttc ccggtcccca tactcaagct ttggcgaaag cggccgatca ccctgttgcg
    10921 gccgttgctt ccgtcggatt tccgtcatcg gcggcggcaa gcttccgccg cagcggcagg
    10981 attccgccgc cgccgagaca ccaaaaaacc gcctctccgg cgtgaaaccc gcatggcagc
    11041 gcgctttcca gccgctggca cgcatattgc tgcttgcccg gcggacacct ttggtggagc
    11101 cgccatgagc aacccatccg acccgctgtt cggcatccat gcccgcgcac tcgacgtgtg
    11161 gcagcggcgt gccgaagcga tcgccggcaa c
//
LOCUS       DASSNH010000186        12320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1318380, whole genome shotgun sequence.
ACCESSION   DASSNH010000186 DASSNH010000000
VERSION     DASSNH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318380"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /gene="putA"
                     /locus_tag="VFK00_06940"
     CDS             <1..2175
                     /gene="putA"
                     /locus_tag="VFK00_06940"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HET7592721.1"
                     /translation="AVQAYQKRAPFVIDWLAETARNAGRRWCVRLVKGAYWDAEIKLA
                     QEQGLAGYPVYTRKPNTDVSYLACARRMFEAGCDLIYPQFATHNAHTIAAIHHMARGR
                     PFEYQRLHGMGKELYDEVIGKQHLDVPCRVYAPVGSHRDLLPYLVRRLLENGANTSFI
                     NHVVDEFLPMEKLVGDPCEVVRAYASKPHPRIPLPVNLYAGLRKNSMGVNLSDDNELA
                     SLAEAANQHPGPWHAAPLVPGATPSGDTVKVTNPADRRVEIGTWQGADTASVDKALAN
                     AVSAQPGWDRLPAASRAAILEYAADQLEARRGEFISMCVREAGKTIGASISEVREAAD
                     FLRYYATMARRLFGQPEALPGPTGETNELSLHGRGVFVCISPWNFPLAIFTGQVSAAL
                     VAGNAVIAKPAEQTTLIAHAMVKLLHEAGVPTDVLQFVPGDGATVGPALTGDNRVGGV
                     AFTGSTDTARAINRALAERDAPIAALVAETGGQNAMIADSSALPEQLVKDVVSSAFDS
                     AGQRCSASRVLYVQDVVADKVIGMLGGAMDELTVGDPALLSTDVGPVIDDDALKMLQA
                     HAARMDKQAKLIREVKLPEACAHGTFFAPRAYEIASLAVLEREVFGPVLHVVRWKAGE
                     LDKVIDQVNATGYGLTLGIHSRIDETVDYIQARVRVGNCYVNRNQIGAVVGVQPFGGE
                     NLSGTGPKAGGPHYLLRFATERTFTVNTTAAGGNASLLTLQD"
     gene            2375..2806
                     /locus_tag="VFK00_06945"
     CDS             2375..2806
                     /locus_tag="VFK00_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HET7592722.1"
                     /translation="MIGRSNHRGIATAVALGIAMLAPAAFAQTQPATQQQTPPPAGAA
                     ASQSDTGATPNDTELKHFADAVVEVQGIRQNLQPQLANAKTEDARTQLKADAEKKMES
                     AVRSNQLSLDRYVQIAKLVQTDSQVRARVQQLMPPQEPSKS"
     gene            complement(2915..4276)
                     /locus_tag="VFK00_06950"
     CDS             complement(2915..4276)
                     /locus_tag="VFK00_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET7592723.1"
                     /translation="MSNKPISASGLAGLALAALAVAVSGALVSPVASASGFQISENTV
                     KAMGRAYAGREAAGGDASVVINNPAAMVDFDTYAVQIDATAISVSTQFHGGGTDAIGQ
                     PLSGGDGGDGGGVHGVPAISFIAPLGPDWRIGFGVNAPYGLATEYDAGWMGRYQALKS
                     GVKSMDFTGSIAWAVAPQFSLGFSVIYQRTSVDIGNAVNLGAVLAAPPFNLAPAFLPQ
                     SADGAVRLRGDDSKWGWQIGAEWKPTTQDTIGFSYHAKINHDIDGTAYFSVPPDVQYV
                     LSQPGVPPLFQTTGGRGELATPATANLSYWHKTMGPVSWGAELSWTGWSSFQRLAIDF
                     ANPAQPDIDQYYGWKDTLFGSLGMDYQVSDRWTWRAGVAYDQTPTRDASRDPRIPDAA
                     RKWISLGATYSPSAQVEFNVGYTHLFVSDSSVDDISATGDHLVGSFENSGNLFGISMQ
                     YKF"
     gene            4536..7985
                     /locus_tag="VFK00_06955"
     CDS             4536..7985
                     /locus_tag="VFK00_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7592724.1"
                     /translation="MPIAKSRRRPPLSRSLACFLPSLVAVLLAIWIPPDWERYSLVLL
                     LVGASLSMAAACHAIGFGCEATFVRTALRRGPAHLIALVIHTAVVFVLVAWPLLVLSR
                     TPSLPATLGLCVALVIALVVLWRFWPAFGLVFEWDDAFPAAAEHSWITTALARSARFA
                     QHLTRRRDLFLTHFLPASIAQLAIAFGALTLAGLGADLPDEIRTAALFLFAVVVLPVC
                     SMVVANRTLRLLFDRRSPRAGPPPEAPPPREPERHVASAENAEHEASNETAAPDVAPG
                     APAEPTVPQTSLDEGLLAAARAGDAGAASDWLARGAHPDAAPPAGARDQRSALVLAAE
                     LPDTRLLRALIGAGAQVNRAQAGLTPLLAATRCCSAGRPETVTSLVANGADPALADAD
                     GNTPLHHAALCSEPAVAAILVDAQAPLEARNRDQATPLAVAAEAANWPLLRFLVRRGA
                     QPGAALPAAAGIADDDPEGVEFLLKHKAPVDAVDALGRSALMHAAREGHAAIVRSLLD
                     AGADAALADRHGTTPLMEAARAGATAVVALLAEKRPDIHAGDQHGRTALVLACQSPRA
                     SVDCIRALLAMGADPCRKGPDGRSAVDHASAAGRWDLVALLDPEAPLPASHDHGSQPE
                     AGADTPAHLLDALRFAHWNVVARFAERVQSWPVNELAALYLDLAGHDDRAPRAWLLEH
                     GLAADATLDDGSRLFDAVLDRLPNSACAVWQLLDAGATPAGAGRYAHALGRLGGAADG
                     ATLALALLERGADLFGTDSDGRSPLHHASRPALLPVLEALLARGADPNVRDRAGATPL
                     HVALDADPEAALPQVKALIAHGADPECPAAGGETPLGLALARGATQLEYWLRWNGWPL
                     PGRPLHAHDLPAAASDPEAVARLLALGMPVDARDARGATALLRACGAGSLQSVQRLLA
                     AGADPAYTLDTGASPLSAAASARHPDILRALVAAGVAVDHRHAEGVTALMIAAALGNP
                     EVCAVLRELGAQPDAADVHGRTPLHAGARFCFDSRDSLRCRRLLDSLLPGCGALNSAD
                     VSGATPLLMLLGVHVKPGTDCDGTHLGALLPVLLDAGADPAHADEHGVTALHACAMHA
                     LFEPARALLARGADRDARDCRDRTPADVARVLGFVDLALELSPRRGAVNPSVVTPLMT
                     HPE"
     gene            complement(7942..9780)
                     /locus_tag="VFK00_06960"
     CDS             complement(7942..9780)
                     /locus_tag="VFK00_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HET7592725.1"
                     /translation="MQGHSLLQSVLVFLVAVVIAVPLARRLKLGSVLGYLAAGVVIGP
                     YGLHWVRDPEAISGISEFGVALLLFVIGLELSPQRLWVMRRQVFGAGAAQVFGTAIVI
                     ALIAALGFHLDAMAAAVVGLGLAFSSTAFGLQILAERRELQSPHGRQAFAMLLFQDLA
                     AIPLIAAVPLLAAHGATHAPGQPLWLSALRVAGVIAVVVVGGRYALRPVFRAVAKTQL
                     IEVSTATALLVVIGTAWLMQLAGISMALGTFLAGVLLADSEYRHDLESNIAPFEGLLL
                     GLFFITVGMGVNLKLLALHPWIVLGLVALLLACKGPIVAAVGKANPRLDGRDALKLAA
                     LLAGGGEFAFVVFRLAAANGMLTRAQQELLVLVVTLAMAAVPLLVLAAARMPASRKPE
                     RAYDEIDADGPRVIIAGFGRVGQIIGRLLRARGIPFVALENSIEQVDLSRRFAGTNIY
                     FGDPSRAEILRAAQAGKAEVFVLATDDPATNVRTARMVRRMFPHLKIIARARNRQHAF
                     RLMDLTSDPDDVVRETFYSSLKMARLALTALGVAPAQAEASVTMFREHDEKLLRDQHL
                     VYDDDVALRQTTREAYAELQGLFEADVAAEAVPTRDASSTASPPTG"
     gene            complement(9875..11254)
                     /locus_tag="VFK00_06965"
     CDS             complement(9875..11254)
                     /locus_tag="VFK00_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HET7592726.1"
                     /translation="MQRVLGLSADRRGRRALPLTPVMRAAWSGFAKTLGSRQRRWAEA
                     CGFIGQAGAICMLPDARGDSARVLCGIDPALPPFALGGLPWQLPADDYRIEGDVLSAH
                     DAALGWALGSYRFARYREPHAEPARLVLPRAVLDEVSPTVEAIARVRDLVNTTAQDLG
                     PAEVCAVVREVGAAYGAKCREWSGDGLLEAGFPTVHAVGRASPRAPRVVELTWGDPKH
                     PLLALVGKGVCFDTGGLDLKSHDGMRWMKKDMGGAAHALSLAQLVMASNLPVRLRLLI
                     PTVDNAVAGDALRPGDVIRTRAGITVEVDNTDAEGRLILCDALAAAVEEKPDLLIDFA
                     TLTGAARVALGPDLPALFASRDDLAEAVLAAGAAQHDPLWRLPLWQPYRAMLVSGVAD
                     TSNTGASRHAGAITAALYLQNFVPASVPWLHVDVYAWNDGDRPGRPRGGDAQSLRAFH
                     AFLRERYRA"
     gene            complement(11277..>12320)
                     /locus_tag="VFK00_06970"
     CDS             complement(11277..>12320)
                     /locus_tag="VFK00_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7592727.1"
                     /translation="TIEGIDQALAGTIDIAASARPAYPKRTQEAGLTFTPVAWDALVM
                     ITNASNPVNNLTLKQLHDIYYGKITNWSAVGGRDEKIDLDGIASPLDGVQFSFRRLMF
                     GNGDNPVAVPRLFINIDSLQTEVSLDTKALALSTMVHAQRQKGIKVIPIEGVSPSLAT
                     LENATYPLPVKIYLAYKSDNPKVAAIEQFLEFLQGAAADGILRDHGLLPFHQAFVLNA
                     RSEADRINTLAARMIAEGLPPEYSPSTEFARLAEIYPDLAHTLRLQQVAAEQVAQANQ
                     QRIALAEAAGVDPSPHASEATAKPETYTVVSGDTLSKIAQQHSVTVDELRKWNHLDSD
                     TLSIGQELKVSSQ"
BASE COUNT         1914 a         4406 c         4166 g         1834 t
ORIGIN      
        1 gccgtgcagg cctaccagaa gcgcgcgccg ttcgtgatcg actggctggc cgagactgcg
       61 cgcaatgccg gccgccgctg gtgcgtgcgg ctggtcaagg gcgcgtactg ggatgccgag
      121 atcaagctgg cccaggaaca gggcctcgcc ggctatccgg tgtacacgcg caagcccaac
      181 accgacgtgt cctacctcgc ctgcgcccgc cggatgttcg aggctggctg cgacctcatc
      241 tatccgcagt tcgcgaccca caacgcgcac acgatcgccg cgatccacca catggcgcgc
      301 ggccgtccgt tcgagtacca gcgcctgcac ggcatgggca aggagctgta cgatgaagtg
      361 atcggcaagc agcacctcga cgtgccctgc cgcgtgtacg cgccggtcgg cagccaccgc
      421 gacctgttgc cgtacctggt gcgccggctg ctggaaaacg gcgccaacac cagcttcatc
      481 aaccatgtgg tcgacgaatt cctgcccatg gagaagctgg tgggcgatcc ctgcgaagtg
      541 gtgcgcgcct acgcatccaa accgcacccg cgcatcccgc ttccggtcaa tctctacgcg
      601 ggcctgagga aaaattcgat gggcgtgaac ctgtccgacg acaatgaact cgcgtcgctc
      661 gccgaagcgg ccaaccagca tccgggaccg tggcacgcgg cgccgctggt acccggcgcc
      721 acgccatccg gcgatacggt gaaggtcacg aacccggccg accgcagggt cgaaatcggg
      781 acctggcagg gcgccgacac cgccagcgtg gacaaggcac tcgcgaacgc ggtgtccgcc
      841 cagccgggct gggaccgcct gccggccgcg agccgcgccg cgatcctcga atacgccgcc
      901 gaccaactgg aagcgcgccg cggtgaattc atctcgatgt gcgtgcgcga ggccggcaag
      961 acgattggcg catccatttc cgaagtgcgc gaggcggccg acttcctgcg ctactacgcg
     1021 acgatggcgc ggcggttgtt cggccagccg gaggcgctgc ccggaccgac gggcgaaacc
     1081 aacgagctgt cgctgcacgg gcgcggcgtg ttcgtgtgca tcagcccgtg gaatttcccg
     1141 ctggcgatct tcaccggcca ggtcagcgcg gcgctggtcg ccggcaacgc cgtgatcgcc
     1201 aagccggccg agcagaccac gctgatcgcc catgcaatgg taaagctgct gcacgaggcc
     1261 ggcgtgccga cggacgtctt gcaattcgtc cccggcgacg gcgcgacggt gggccccgca
     1321 ctcaccggcg acaaccgcgt cggcggcgtc gcgttcaccg gctcgaccga tacggcgcgc
     1381 gcgatcaatc gcgcgctcgc cgaacgcgac gcgccgatcg ccgcgctcgt cgccgaaacc
     1441 ggcgggcaga acgcgatgat cgccgattcc tcggcgctcc cggaacaact ggtcaaggac
     1501 gtcgtcagct cggcgttcga ttccgccggc cagcgctgct cggccagccg ggtgctgtac
     1561 gtgcaggacg tggtcgcgga caaggtcata ggaatgctgg gtggcgcgat ggacgagctc
     1621 accgtgggcg atccggcgct gctctcgacc gacgtcggcc ccgtgatcga cgacgacgcg
     1681 ctcaagatgc tgcaagcgca cgccgcgcgg atggacaagc aggcgaagct gatccgagaa
     1741 gtgaaactgc cggaagcctg cgcgcacggc accttcttcg cgccgcgcgc ctacgagatc
     1801 gcgtcgctgg cggtgctcga gcgcgaagtg ttcggcccgg tcctgcacgt cgtgcgctgg
     1861 aaggccggcg aactcgacaa ggtcatcgac caggtcaacg ccaccggcta cggcctcacc
     1921 ctcgggatcc acagccgcat cgacgaaacc gtcgactaca tccaggcgcg ggtgcgcgtc
     1981 ggcaactgct acgtcaaccg caaccagatc ggcgcggtgg tcggcgtgca gccgttcggc
     2041 ggcgagaacc tttccggcac cggtcccaag gccggcggcc cgcactacct gctgcgcttc
     2101 gcgaccgagc gcacgttcac cgtcaacacc acggccgccg ggggcaatgc gtcgctgctg
     2161 acgctgcagg attgaggcca agtcgcagcg acgcaacgct ttctttaccg ccgcgtaatg
     2221 cgttcgttta cgagtcgttc gaaaattcgc gcgaaacctg cctgagtctg aattgccgtc
     2281 cggcaaggcg ttgcacccgt atcgcattcc tcgctaggtt gaaatcgcgg cgtctcccag
     2341 cgccgcacgc atttccactg acgaggacaa cgcaatgatt ggacgatcca accaccgggg
     2401 catcgcaacg gctgtggcgc tggggatcgc catgttggca cccgcggcat tcgcacagac
     2461 ccaaccggca acgcagcagc agaccccgcc tcctgccggc gcggccgcgt cgcaatccga
     2521 caccggagcg acgccgaacg atacggagct gaagcatttc gcggacgccg tggttgaagt
     2581 gcaaggcatc cgccagaact tgcagcccca gctcgcgaac gcgaagaccg aggatgcccg
     2641 cacgcaactg aaggcggacg ccgagaagaa aatggaaagc gcggtgcgca gcaaccagtt
     2701 gtcgctggat cgctacgtcc agatcgccaa gctggtgcag accgacagcc aggtccgtgc
     2761 gcgggtccag caactgatgc cgccgcagga accctccaag tcctgacgcg gcgcgctttg
     2821 gaatgtcgct ttcccttccc gcgggaggcg gatgcaaacg aaaaccccgc ctttcggcgg
     2881 ggttttcctt tcagcccatg ccgcgacgca cccgtcagaa cttgtactgc atcgaaattc
     2941 cgaacaggtt gcccgagttc tcgaagctgc cgaccagatg gtcgccggtc gcgctgatgt
     3001 cgtcgacgga actgtcgctg acgaacaggt gggtgtagcc gacgttgaac tcgacctgcg
     3061 cgctcggcga atacgtcgcg ccgagcgaga tccacttgcg cgcggcgtcc gggatgcgcg
     3121 gatcgcgcga agcgtcccgc gtgggcgtct ggtcgtaggc cacgcccgcg cgccaggtcc
     3181 agcggtcgct gacctggtag tccatgccga gcgaaccgaa caacgtgtcc ttccagccgt
     3241 agtactggtc gatgtcgggc tgcgccggat tggcgaagtc gatcgcgaga cgctggaagg
     3301 aactccaccc ggtccaggac agttccgcgc cccaggacac cggacccatg gtcttgtgcc
     3361 agtagctcag gttggcggtg gcgggcgtcg cgagttcgcc cctgccgccg gtggtctgga
     3421 acaacggcgg cacgcccggc tgcgacagca cgtactgcac gtccggcggc acggagaagt
     3481 acgcggtacc gtcgatgtcg tggttgatct tggcgtggta gctgaatccg atggtgtcct
     3541 gcgtggtcgg cttccactcg gcgccgatct gccagcccca cttggagtcg tcgccgcgca
     3601 ggcgcaccgc gccgtcggcg gactggggca ggaaggcggg cgcgaggttg aatggcggcg
     3661 ccgccagcac cgctccgaga ttgacggcgt tgccgatgtc gacgctggtc ctctggtaga
     3721 tcaccgaaaa gcccagcgaa aactgcggcg cgaccgccca cgcgatcgaa ccggtgaaat
     3781 ccatcgactt cacgccggac ttcagcgcct ggtagcggcc catccagccg gcgtcgtatt
     3841 cggtcgcgag accgtacggc gcgttgacgc cgaaaccgat gcgccagtcc ggcccgagcg
     3901 gcgcgatgaa actgatggcc ggcacgccat ggacgccgcc gccatcaccg ccgtcgccac
     3961 cggacagggg ttgcccgatc gcatcggtgc cgccgccgtg gaactgggtg ctgacgctaa
     4021 tcgcggtggc atcgatctgc accgcataag tgtcgaaatc gaccatggcc gcgggattgt
     4081 tgatcaccac ggacgcatcg ccacccgccg cctcgcgccc ggcataggcg cgtcccatcg
     4141 ccttgacggt gttctcgctg atctggaagc cggaagcgga cgcgaccggg ctgaccagcg
     4201 cgccggatac ggcgacggcc agggcggcca gcgccagccc ggccagccct gaagcggaaa
     4261 tcggtttgtt gctcatcgat ggatctccag tcgaaagttg atggacctgc ggcatggacg
     4321 gcctcggacc tcgggcgctc cgtctcgttc atacgtttgt atgaaatttt tgttatggcg
     4381 actatcgcat cgtggcaggg cccgcgcaag tagccgcagt gtcttcttcc atgcgcgggg
     4441 aaccttgcgg cggggcgttg ggggtagcca tccttccgtg tggcccgtaa cgccgcattc
     4501 cctatacttg gccgctctgt cgcacgggtc cgttcatgcc tattgcaaaa tcccgccgcc
     4561 ggccgcccct ttcgcggtcg ctggcctgtt tcctgccttc gctggtcgcg gtgttgctcg
     4621 cgatctggat cccgcccgac tgggaacgct attcgctggt gctgctgctg gtcggcgcgt
     4681 cgctgtcgat ggcggccgcc tgccacgcga tcggtttcgg ttgcgaagcg acgttcgtgc
     4741 gcaccgcact ccggcgcggc cctgcgcacc tgattgcgct cgtgatccac accgcggtcg
     4801 tgttcgtgct ggtggcgtgg ccgctgctgg tgctttcacg cacgccgtca ctcccggcga
     4861 cgctcggatt gtgcgtggcg ttggtgatcg ccctggttgt gctgtggcgc ttctggccgg
     4921 cgttcggctt ggtgttcgaa tgggacgatg ccttccccgc cgccgccgaa cattcgtgga
     4981 tcaccaccgc gcttgcgcgc agcgcccgct tcgcgcaaca cctcacgcgc cggcgggacc
     5041 tgttcctgac gcacttcctg ccggcctcga tcgcgcagct cgcgatcgcc ttcggcgccc
     5101 tcaccctcgc gggtctgggt gcggacctgc cggatgaaat ccgcacagcc gcactgttcc
     5161 tgttcgcggt ggtggtactg ccggtgtgtt cgatggtcgt cgccaaccgg accctgcggc
     5221 tgttgttcga ccgccgcagt cctcgcgccg gtccgccacc cgaagcgccg ccgccacggg
     5281 agccggaacg acacgtcgcg tccgccgaaa acgcggaaca cgaggcgtcg aacgaaaccg
     5341 cggccccgga cgtcgcgcct ggcgcgccgg ccgaaccgac cgttccccaa acctcgctgg
     5401 acgagggctt gctcgcggcc gcgcgcgcgg gtgacgccgg cgccgccagc gactggctgg
     5461 cgcgcggcgc ccacccggat gcagcaccgc ccgcgggtgc gcgcgaccag cgttccgcgc
     5521 tggtcctagc ggccgaattg cccgacaccc ggttgctccg cgccctgatc ggcgcgggcg
     5581 cgcaggtgaa ccgcgcgcag gcggggctca cgccgctgct ggccgcgacc cgctgttgca
     5641 gcgcgggccg gccggaaacg gtcaccagcc tggtcgccaa cggcgccgac ccggcactcg
     5701 ccgatgcgga cggcaacaca ccgctgcacc atgcagcgct ttgcagcgaa ccggccgtcg
     5761 cggcgatcct cgtcgacgcg caggctccgc tcgaggcgcg caatcgcgac caggcgacgc
     5821 cgctcgcggt ggccgccgaa gccgcgaact ggccgctgct ccggttcctg gtccggcgcg
     5881 gcgcacagcc cggcgccgcg ctgccggccg cggccgggat cgccgacgac gaccccgaag
     5941 gcgtcgaatt cctgctcaag cacaaggcgc cggtggatgc ggtcgatgcg ctcggccgca
     6001 gcgcgctgat gcacgccgcg cgcgaaggcc atgccgcgat cgtgcgcagc ttgctcgacg
     6061 ccggagccga tgccgcgctc gccgaccgcc acggcaccac gcccttgatg gaagccgcgc
     6121 gggccggcgc caccgcggtc gtcgcgctgc tggcggaaaa gcgacccgac atccacgccg
     6181 gcgaccagca cggccgcacg gcgctggtcc tcgcctgcca gtcgccgcgc gccagcgtcg
     6241 attgcatccg cgcgctcctc gccatggggg ccgatccgtg ccgcaagggc ccggatgggc
     6301 gcagtgcggt cgaccacgcc agcgcagccg gccgctggga cctggtcgcg ctgctcgatc
     6361 ccgaggcgcc acttcccgcc agccacgacc acggcagcca gccggaagca ggcgcggaca
     6421 cgcctgcgca cctgctcgac gcgctgcgct tcgcgcactg gaacgtggtc gcgcgattcg
     6481 cggagcgcgt gcagagctgg cccgtgaacg aactcgccgc gctgtacctc gacctcgccg
     6541 gacacgacga tcgcgcgccg cgtgcctggc tgctcgaaca cggcctcgcc gccgacgcca
     6601 cgctcgacga cggcagccgg ctgttcgatg cggtcctcga ccgcctgccc aattcggcct
     6661 gcgccgtgtg gcaactgctg gatgcaggcg cgacgccggc gggcgccgga cgctacgcgc
     6721 acgcgctcgg ccggctcggc ggcgccgcgg acggcgcgac gctcgcgctg gccctgctgg
     6781 agcgcggcgc ggacctgttc ggcaccgatt ccgatggacg ctcgccgctg caccatgcca
     6841 gccgacctgc gctgctgccc gtgctcgaag ccctgctcgc gcggggcgcc gatccgaacg
     6901 tccgcgaccg cgcgggggcg acaccgctgc acgttgcgct cgacgccgac ccggaggcgg
     6961 cgctgccgca ggtcaaggcg ttgatcgcgc atggcgccga tcccgaatgt cccgcggccg
     7021 ggggcgagac gccgctcggc ctcgcactcg cgcgtggcgc gacgcaactc gaatactggc
     7081 tgcgctggaa cggctggccg ctgcccggtc ggccgttgca cgcacacgac ctgccagccg
     7141 ccgcgagcga tcccgaggcg gtcgcacgct tgctggcgct cggcatgccg gtggatgcgc
     7201 gcgacgcgcg cggcgccacc gcactgctgc gggcctgcgg ggccggttcg ctgcaaagcg
     7261 tgcagcgcct gctggccgcg ggtgccgatc cggcgtacac gctggacacc ggcgcctcgc
     7321 ctctgtccgc cgcggcgagc gcgcggcacc cggacatcct gcgcgcgctc gtcgccgccg
     7381 gcgtggcggt cgaccaccgt cacgccgagg gcgtcactgc actgatgatc gccgccgcgc
     7441 tcggcaatcc cgaagtctgc gcggtgctgc gcgaactcgg cgcccagccg gatgccgccg
     7501 acgtgcacgg ccgcacgccg ctgcacgcgg gcgcgcggtt ctgcttcgac agccgcgaca
     7561 gcctgcgctg ccggcggctg ctggacagcc tgctccccgg atgcggcgcg ctcaacagtg
     7621 ccgacgtgtc tggcgccacg ccactgctga tgttgctggg cgtgcacgtg aagcccggaa
     7681 ccgattgcga cggcacccac ctcggcgcgc tgctgccggt gctgctcgat gccggcgcgg
     7741 atcccgccca cgccgacgaa cacggtgtca ccgcactgca cgcctgcgcg atgcacgcgt
     7801 tgttcgagcc ggcacgggcg ctgctggccc gcggcgccga tcgcgacgcg cgcgactgca
     7861 gggaccgcac gccggccgac gtcgcgcgcg tgctcggctt cgtcgacctc gcgctggaac
     7921 tttcgccgcg tcgcggcgcg gtcaacccgt cggtggtgac gccgttgatg acgcatcccg
     7981 agtagggacg gcttccgccg cgacgtcggc ttcgaacagt ccctgcagtt ccgcgtacgc
     8041 ctcgcgggtc gtctggcgca acgccacgtc gtcgtcgtac acgaggtgct ggtcgcgcag
     8101 cagcttctcg tcatgctcgc ggaacatcgt cacgctcgcc tcggcctgcg ccggcgccac
     8161 cccgagcgcc gtcagcgcca ggcgcgccat cttcaggctc gagtagaacg tctcgcgcac
     8221 gacgtcgtcg ggatcggagg tgaggtccat caggcggaac gcgtgctggc ggttgcgcgc
     8281 acgcgcgatg atcttcaggt gcgggaacat ccggcgcacc atgcgcgcgg tgcggacatt
     8341 cgtggccggg tcgtcggtcg ccagcacgaa cacctcggcc ttgccggcct gcgccgcgcg
     8401 caggatttcc gctcgcgagg gatcgccgaa atagatgttg gtgccggcga aacgccgcga
     8461 caggtccacc tgctcgatcg agttttccag cgccacgaac ggaatgccgc gcgcccgcag
     8521 caggcggccg atgatctgcc cgacccgccc gaaaccggcg atgatcaccc gcggtccatc
     8581 cgcatcgatt tcgtcatagg cgcgttccgg cttgcgcgat gcgggcatcc gcgccgcggc
     8641 gagtaccagc agcggcaccg ccgccatcgc gagggtcacc accagcacca gcagttcctg
     8701 ctgggcacgg gtcagcatcc cgttggcggc ggccaaccgg aataccacga acgcgaactc
     8761 cccgccgccg gccaggagcg cagcgagctt gagcgcgtcc ctgccgtcca gccgcgggtt
     8821 ggccttgccc accgcggcca cgatcggacc cttgcaggcg agcagcaacg ccaccagtcc
     8881 gagcacgatc cacgggtgca gggcgagcag cttcaggttc acacccatcc cgaccgtgat
     8941 gaagaacagc ccgagcagca ggccctcgaa cggcgcgatg ttggattcga ggtcgtggcg
     9001 gtattccgaa tcggcgagca gaacgccggc caggaaggtg cccagcgcca tcgagatccc
     9061 ggcgagctgc atcagccagg cggtgccgat caccaccagg agcgcggtgg cggtcgacac
     9121 ctcgatcaac tgggtcttgg ccaccgcccg gaacaccggc cgcagcgcat agcgcccgcc
     9181 caccaccacc accgcgatca cgccggcgac gcgcagcgcc gaaagccaca gcggctggcc
     9241 cggcgcgtgg gtcgcgccgt gcgccgcgag cagggggacc gccgcgatca gcggaatcgc
     9301 ggcgagatcc tggaacaaca gcatcgcgaa tgcctgccgc ccatgcggac tctgcagttc
     9361 gcggcgctcg gcgaggatct gcaggccgaa ggcggtggac gagaacgcca gcccgagtcc
     9421 gaccacggcc gcggccatcg catcgaggtg gaacccgagc gccgcgatca gcgcgatcac
     9481 gatcgcggtg ccgaacacct gcgccgcgcc cgctccgaac acctgccgcc gcatcaccca
     9541 caatcgctgc ggcgacaatt ccaggccgat cacgaacaac agcagcgcga cgccgaactc
     9601 ggagatcccg gaaatcgcct caggatcgcg cacccagtgc aggccgtacg gcccgatcac
     9661 gaccccggcc gcgagatagc cgagcaccga gccgagcttc agacggcgcg cgagcggcac
     9721 cgcgatcacc accgcgacca ggaagaccag caccgactgc agcagcgaat gtccttgcac
     9781 gaatggggaa accggggtca gagtcgactt tcgagagtgt ctcaccaaac acgcgggtct
     9841 gtcgcgaaag tcgactctga ccccggtttc cccgtcaggc gcgatagcgc tcgcgcagga
     9901 acgcgtggaa agcgcgcagc gactgcgcgt cgccgccgcg gggacggcct gggcggtcgc
     9961 cgtcgttcca ggcgtacacg tccacgtgca gccaaggcac gctcgccggc acgaagttct
    10021 gcaggtacag cgccgcggtg atcgcgcctg catggcgcga cgccccggtg ttgctggtgt
    10081 ccgcgacacc cgagaccagc atcgcgcggt acggttgcca cagcggcagc cgccacaacg
    10141 ggtcgtgttg cgccgcgccg gcggcgagca cggcctcggc gagatcgtcg cggctcgcga
    10201 acaaggccgg caggtcggga ccgagcgcga cccgcgcggc gccggtcagc gtggcgaaat
    10261 cgatcagcag gtccggcttc tcctcgaccg ccgcggccag cgcgtcgcac aggatcaggc
    10321 ggccttcggc gtcggtgttg tcgacttcga cggtgatgcc tgcgcgggtg cggatcacgt
    10381 cgcccgggcg cagcgcatcg ccggccacgg cgttgtcgac cgtcgggatc agcagccgca
    10441 ggcgcaccgg aaggttcgac gccatcacga gctgcgccag cgacaacgcg tgcgcggcgc
    10501 cgcccatgtc cttcttcatc cagcgcatgc cgtcgtggct cttgagatcg agcccgccgg
    10561 tgtcgaagca cacgcccttg ccgaccagcg ccagcagcgg gtgctttggg tcgccccagg
    10621 tgagttcgac cacccgcggc gcgcgaggac tggcccggcc caccgcatgc acggtcggga
    10681 aaccggcctc gaggagcccg tcaccgctcc attcacggca cttcgcgccg tatgcagccc
    10741 ccacctcacg gactactgcg cacacttcgg cgggaccgag gtcctgcgcc gtggtgttga
    10801 ccaggtcgcg cacccgcgcg atcgcctcga ccgtcggcga cacctcgtcc agcaccgcgc
    10861 gcggcagcac cagccgagcg ggctcggcgt gcggttcgcg atagcgcgcg aagcggtagc
    10921 tgccgagcgc ccagccgagc gcggcgtcgt gcgccgacag gacgtcgccc tcgattcggt
    10981 agtcgtccgc cggcaactgc cagggcaagc cgccgagcgc gaacggaggc aacgcgggat
    11041 cgatcccgca caacacccgc gcactgtcgc cgcgcgcgtc cggaagcatg cagatcgcac
    11101 ccgcctgccc gatgaaaccg caggcctcgg cccatcgccg ctggcgggat ccgagggtct
    11161 tggcgaatcc gctccaggcc gcccgcatca ccggcgtcaa cggcagcgcg cggcgaccgc
    11221 gccggtcggc gctcaggccg agcacgcgct gcatgccccg gtgcggcggg tccgtgtcat
    11281 tgcgaagata ccttcagctc ctgtccgatg ctgagtgtat cgctatcgag atggttccac
    11341 ttgcgaagtt cgtcgaccgt caccgaatgt tgctgggcga tcttcgagag ggtgtcgcca
    11401 ctgaccacgg tgtaggtctc gggttttgcc gtcgcttcgc tcgcgtgcgg cgacggatcg
    11461 acgccggccg cctccgccag cgcgatccgc tgctggttgg cctgggcgac ctgctcggcg
    11521 gcgacctgct gcaaccgcag ggtgtgggcg agatcgggat agatctccgc gaggcgcgcg
    11581 aactcggtgc tcggcgaata ctcgggcggc agtccttcgg caatcatgcg cgcggcgagg
    11641 gtgttgatgc ggtccgcctc gctcctcgcg ttcagcacga acgcctggtg gaacggcagg
    11701 aggccgtggt cgcggaggat cccgtcggcg gccgctccct gcaggaactc gaggaactgt
    11761 tcgatcgcgg cgaccttcgg attgtcggac ttgtaggcga gatagatctt caccggcagc
    11821 gggtacgtcg cgttctcgag cgtcgcgagg ctgggactga cgccttcgat cgggatcacc
    11881 ttgatgccct tttggcgctg cgcgtgcacc atcgtcgaaa gcgccagtgc cttggtgtcg
    11941 agcgacacct cggtctgcag cgaatcgatg ttgatgaaca ggcgcggcac cgcgaccgga
    12001 ttgtcgccgt tgccgaacat cagccgccgg aaactgaact gcacgccgtc gagcggcgat
    12061 gcaatgccgt cgaggtcgat cttctcgtcg cgcccgccga ctgccgacca gttggtgatc
    12121 ttgccgtagt agatgtcgtg cagctgcttc agcgtcaggt tgttcaccgg gttggaggca
    12181 ttggtgatca tcaccagcgc atcccacgcc accggcgtga aggtgagccc ggcttcctgg
    12241 gtgcgtttcg ggtaggccgg gcgtgcactg gccgcgatgt cgatggtgcc ggccaacgcc
    12301 tggtcgatgc cctcgatggt
//
LOCUS       DASSNH010000187         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_267040, whole genome shotgun sequence.
ACCESSION   DASSNH010000187 DASSNH010000000
VERSION     DASSNH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267040"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VFK00_06975"
     CDS             <1..111
                     /locus_tag="VFK00_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004085459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592728.1"
                     /translation="FDKARGRIVGSEQYLTKPFTRNELLDAIRQHVAAVK"
     gene            146..517
                     /locus_tag="VFK00_06980"
     CDS             146..517
                     /locus_tag="VFK00_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592729.1"
                     /translation="MAHILIIDDSPTDVKIFSGALERAGHKVTAAETAEAGIAAAQKD
                     RPDLVLMDVIMPGMNGFQATRSLTKDPQTSTIPVLIVTTKNMETDRMWGMRQGAKDVL
                     VKPVSEKDLVDNVNKHLAVQA"
     gene            526..1173
                     /locus_tag="VFK00_06985"
     CDS             526..1173
                     /locus_tag="VFK00_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7592730.1"
                     /translation="MGKRRTRENHASRSSGRRHAQTDAPDAAAEDDDVAAVQPEGAFA
                     LLIDYERRSLAHDPGVPEQIEAPGLWRAIGFRVGDRNFVSGIDEISEILMSPPPVTTI
                     PSTRPWLLGVANVRGNLIALVDLKQFLFEQRTHVTGRVRVLVVRQTGGNVGLLVDELL
                     GQRNLTEADRVGAEGEADAQLAKFVTENVTLGSARLGRFSMVELTRAPEFQHAAL"
     gene            1352..>2031
                     /locus_tag="VFK00_06990"
     CDS             1352..>2031
                     /locus_tag="VFK00_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HET7592731.1"
                     /translation="MSAKSGTARQGTSLQTWLIILLLLAIILAGADFFLLNRHDSQDR
                     AAAQLTTQIQVNSQQVANFALQAANGNIDAFKELASTRAAVAAAVQHLNQGDPATGLP
                     AYADAIGVSASMAALTKSWQQLDADLGKIQANKDAVLDSASQAATFNQQMPDLNSKIN
                     QVISILQSRGSSTQIYTASQLMVSGDRLIRRVGDVLQGGADSASAANGLALDSTNYGG
                     TLKQLADSA"
BASE COUNT          391 a          684 c          661 g          295 t
ORIGIN      
        1 ttcgacaagg cgcgcggacg gatcgtgggt tccgagcagt acctgaccaa acctttcacc
       61 cgcaacgaat tgctggacgc gatccggcag cacgtcgcgg ccgtcaaata aggcgcgcgc
      121 cgcgagcgca cgcacgggga aaccaatggc acacattctg atcatcgacg attcgcccac
      181 cgacgtgaag atcttctcgg gtgcgctgga gcgggccggc cacaaggtca ccgcggccga
      241 aaccgccgag gcgggcatcg ccgccgcgca gaaggacagg cccgacctgg tgctgatgga
      301 cgtgatcatg ccggggatga acggcttcca ggccacccgc agcctcacca aggatccgca
      361 gaccagcacc atcccggtgc tgatcgtcac caccaagaac atggaaaccg accgcatgtg
      421 gggcatgcgc cagggcgcca aggacgtgct ggtcaagccg gtcagcgaaa aggacctggt
      481 ggacaacgtc aacaagcacc tcgccgtgca ggcctgaagg cgccaatggg caagaggcgt
      541 acccgcgaaa accacgcttc ccgcagcagc gggcggcgcc acgctcagac cgacgcgccc
      601 gacgcggcgg cggaggatga cgacgtggcc gcggtacagc cggaaggcgc ctttgcgctc
      661 ttgatcgatt acgagcggcg cagcctcgcg cacgatccgg gggttcccga gcagatcgag
      721 gcgcccgggc tgtggcgcgc gatcggattc cgcgtgggcg atcgcaactt cgtcagcggc
      781 atcgacgaga tcagcgagat cctgatgtcg ccgccgccgg taaccaccat tccctcgacg
      841 cggccgtggc tgctgggcgt cgccaacgtc cgcggcaacc tgatcgcgct ggtggacctg
      901 aaacaattcc tgttcgagca gcgtacccac gtcaccggtc gcgtgcgggt gctggtggtg
      961 cgccagacgg gcggcaacgt ggggctgctg gtcgacgaac tgctgggcca gcgcaacctg
     1021 accgaggcgg atcgcgtcgg ggcggaaggc gaggctgacg cccagctcgc gaaattcgtg
     1081 accgagaacg tgacgctggg gtcggcgcgg ctgggtcgct tcagcatggt ggagttgaca
     1141 cgcgcgccgg agttccagca cgcggcgctt tagttgtgcg atcgatccct gatcgcggat
     1201 tcgttgggtg ctttggggcg ttggagcggt tgcaggcgat gaacaccaga acggtcatcc
     1261 acggtccgac tttcaacaac agccgctccg aatcagctca cggcggggca caagcgatgc
     1321 aagcgacata catggatatg aggtggcaat catgagcgcg aaatccggta cggctcggca
     1381 gggtacgtcc ctgcagacct ggctgatcat cctgttgctg ctggcgatca tcctcgccgg
     1441 tgccgacttc ttcctgctga accggcacga cagccaggac cgtgcggccg cgcagctcac
     1501 cacgcagatc caggtgaact cgcagcaggt cgcgaacttc gccctgcagg cggccaacgg
     1561 caacatcgac gccttcaagg aactggccag cacccgcgcc gcggtcgccg ccgccgtgca
     1621 acacctcaac cagggcgatc ccgcgaccgg cctgccggcc tacgccgacg cgatcggggt
     1681 cagcgcatcg atggctgcgc tgacgaaatc ctggcaacaa ctggacgccg acctcggcaa
     1741 gatccaggcc aacaaggacg cggtgctcga ttcggcttcg caggccgcga ccttcaacca
     1801 gcagatgccg gacctcaact ccaagatcaa ccaggtcatc agcatcctgc agtcgcgcgg
     1861 gtcctccacc cagatctaca ccgcttcgca actgatggtc tcgggcgacc gcctgatccg
     1921 ccgcgtcggc gacgtgctgc agggcggcgc cgattcggcg tcggccgcca acggcctcgc
     1981 gctcgactcg accaactacg gcggcaccct gaagcagctc gccgactccg c
//
LOCUS       DASSNH010000188        23016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_574360, whole genome shotgun sequence.
ACCESSION   DASSNH010000188 DASSNH010000000
VERSION     DASSNH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 23016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23016
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574360"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="tilS"
                     /locus_tag="VFK00_06995"
     CDS             <1..1074
                     /gene="tilS"
                     /locus_tag="VFK00_06995"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7592732.1"
                     /translation="RCAIDTSSGEGVEAAARRARYAALAGSLRDGEWLALAQHRDDQI
                     ETVLLKLLRGAGPEGLGGMRPTRPLGPGLLWRPLLETNRDELARYLIQHEIASLDDPG
                     NQDPRFARNLLRHEILPAIKRHLPHAGSAILHSARLCRVASDHLARDADGALARVRRD
                     DATLDADAWHALPEAVRALVLDRWLREQGLPAPADAQRLELERQVAGAAEDAGPCVGW
                     DGAEVRIWDGRLHASRPLPPLPGDWRSAWEGGILALPKGCGTLVTDGGEPRSAPTFDP
                     PLEVRLRRGGERIKPAGDPHTRELRALFQQARIPPWLRVRCPLIYAGDELVAVADRWT
                     SAHGNAIFDACNVRPRWKRPAIG"
     gene            complement(1175..2446)
                     /locus_tag="VFK00_07000"
     CDS             complement(1175..2446)
                     /locus_tag="VFK00_07000"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7592733.1"
                     /translation="MDDPARRPHVVILGGGFGGLWATRALARVPVRVTLIDRANHHVF
                     QPLLYQVAAASLSGPDIAAPLRHIVRHQDNVTVLMEEVQGIDVAGRSVRTGAGDYEYD
                     WLVVATGSTHAYFGHDDWEPYAPGLKTMDDALAIRRRILSAFEIAEREPDPERRAEWL
                     SFVIIGAGPTGVELAGTLAEIAQHTLAHEFRHADPRHATIHLVEAGPRVLGAMPERLS
                     AAARRKLESMGVRVHVDAAVTEVDETGVRMGDQRIAARTLLWAAGVAASRLGTKLASE
                     LDRHGRVMVAPDLSLPGHPEVLVIGDLAHVEQDGKLVPGMASAAKQMGSHAACVIRDR
                     LDGGSSRPFRYRDYGSLASVSRASAVVDLRGVQMSGFTGWLFWLFAHILFLIGFRNRF
                     AVMWNWAWSYLTWQRNARIIVGRDHDRSAAE"
     gene            2555..4711
                     /locus_tag="VFK00_07005"
     CDS             2555..4711
                     /locus_tag="VFK00_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7592734.1"
                     /translation="MQRRHSWALLALLLLAGAAPAAHSAAPAPDAQGFTLQQVLAYPY
                     PEGLAASKAGNRIAWVIDQNGVRNVWVAQGPAFEPQQVTRFTNDDGQEITQLTFSPDG
                     NDLVFVRGGDHDANWPEKLPPDPAANPAEPKVMLWVVDLGDGTSRALTEGDEPAISSQ
                     GKLAYTRDHQVWTASLAGGDHAKPKRLFFDRGKDGDLQWSPDGKRLAFVSDRGDHAFI
                     GVYTDGDAPLEYLAPSTHNDGMPRWSPDGTRVAFTRRDGDGGPPKPILEQTPEPWAIW
                     VADAGTGEAHALWQSPDTAFGSYPEAPGGANLHWADGGARLVFLADLDGWPHLYSITA
                     NGGTPLLLTPGKFMVEDVVLSRDGRSLIYNANAGESAGDVDRRHLFKVPVDAAKPVPL
                     TSGDNLEWAPTAADANTIAYVEAGSRKPPMLAVMHIDGTGQRLLQPELLPEAFPSGQL
                     VVPKPVTFTAADGTLIHGQLFRSTDASAEQPGVIFVHGGPPRQMLLGWHYMGYYSNSY
                     AVNQYLATHGFTVLSVNYRLGIGHGHAFHHPAHWGPTGASEYQDVVAGAKYLQHVPGV
                     DASRIGIWGGSYGGYLTALALARNSDIFKAGVDMHGIHDWSRDIAEWFDKPDGRYEKG
                     DYQQAMKVAWESSPDASIAEWTSPVLLIQGDDDRNVHFLQMEDVVPRLRKHHVPFEEM
                     VLPNEIHGFLRHASWLKADTATVDFLERKLDANGEH"
     gene            4711..5598
                     /locus_tag="VFK00_07010"
     CDS             4711..5598
                     /locus_tag="VFK00_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET7592735.1"
                     /translation="MNPAPAGEAAVDRARSAREAPGADSEMIDLDAYLRRIGLQTSVA
                     PNPQTLRAIVAAHVAAIPFENLDPFLGITPSLDIASVQQKLVRNGRGGYCFEHNRLLS
                     DALRAIGFDVTDLGARVLWGQSEDAITARSHMLLRVEIEGRSWLADAGFGGNTPTAPL
                     ELVPDIEQATPHEPYRLIRRSDSDWRLQLFAADAWQTLYRFDLQPQFPADYRVSNYWT
                     STNPDSHFVSGLTAARAPAGRRLALRNRQFTEYTTGGGTTRRTLATTAEIRAILQDEF
                     LIPLPERPKLGEMLDRLPE"
     gene            5802..7613
                     /locus_tag="VFK00_07015"
     CDS             5802..7613
                     /locus_tag="VFK00_07015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592736.1"
                     /translation="MAVARRLFAELRRRNVLRAAVLYVGAVWALAQGVAQLGPTFGAP
                     DWTTRAFVIAAAVGFPFWIAFAWIFEFTPQGLKRESEVTEDESLAHWAGRRLDFAIIA
                     VLSVAVALLLGNQFLWHKGAAQGPAASVGAAAKIPQKSVAVLPFANESGKAGEQFFSD
                     GLSEDLITALSQVDGLKVISRNSAFQFRDSKENAKAIGGKLGVAHLLEGSVQHVEEAV
                     RITATLVNTADGSIVWSQRYDKPYRDLFSLQDEITGAVASALKARLLSARGAVVQSDR
                     PPSGSLAAYAAYKHGRAYGALGTEADLRRTIDAFDEATRLDPHYAAAYARLSLAWNAL
                     AAQALGGKAAAEANAQARNAVNTALSLDPNSALAHRAHAYLLLNVDMDWTAVEAETRR
                     ALQLAPNDAVARFDHGVALASLGQVQAAVTLTRQSLDTDPRDDDSFYWLASYLAGLGR
                     LDEAREAINTAIALQPRADGYRALLATIEVLRGDAKAARVAAKQESPGGLWHDTAVAM
                     ASQIGPDRHLADAVFDKLVAGHAEDGAYQIAQIQALRRDADQMFQWLERAWTNRDPGV
                     GYLLSDPFLLRFQHDPRFAAFCKKVGLPTTTDAKAMP"
     gene            7727..8371
                     /locus_tag="VFK00_07020"
     CDS             7727..8371
                     /locus_tag="VFK00_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7592737.1"
                     /translation="MAAKASPLRQRQAQQVRVALLEAAIRQLEGTGDVLMAEVAEAAG
                     VSLRTLYRYFPDRASLLGAAGDHLYRSLGVPFGIAGPDNIAASFREAARRLGTRPLLA
                     RALVRSEAGTLSRSSVRRDRLEAIRDALEPLTEALDPCTAQRASAVIAHLCSASSWVG
                     IADEGGLSDSDAHETVAWAIDTLVATLRKEARRSRPHRNRQQAPRNRRTNHEPD"
     gene            8358..8900
                     /locus_tag="VFK00_07025"
     CDS             8358..8900
                     /locus_tag="VFK00_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7592738.1"
                     /translation="MSQTDHLLHDCGNGDLYTEPERSRAWWFLGTLAVLRNPRGAPTC
                     PTIIELTVPPGGSPPRHIHESLDDNFLLLDGQVVVRCGEQTFVARPGTYVTLPHGVDH
                     TFRVTSALPARMLLLHASDEFLRFIEAAGTPTHERRLPPPGEHDVSRDVLIRLAGEHG
                     VRIIGAPMADDETRRLVESA"
     gene            9047..9484
                     /locus_tag="VFK00_07030"
     CDS             9047..9484
                     /locus_tag="VFK00_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592739.1"
                     /translation="MTDATSEYLAGCHCGALTARFRTALATSAWQVRGCQCSFCRAHA
                     GLTISDPSGSLEFAAVHPEQVQRYRFGRRVTDFLLCRTCGIYVGACVAGPNGRFGVLN
                     AHALRPIPANLPQPVPMDYAAETLLEKQQRREARWTPLTASSL"
     gene            complement(9465..9887)
                     /locus_tag="VFK00_07035"
     CDS             complement(9465..9887)
                     /locus_tag="VFK00_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592740.1"
                     /translation="MTRSGCHRRLDQGYHDARLVALSAHGALEQGRYAELGTDPALSF
                     CASFLEHATLPPSSEASGFNRVLQMRQPKRVYMSPNDKQKAKSGGVRAACFIISPQSP
                     RMSDFGRGSSVTGRPGADGHDVGLGVATSLCLLTGSMP"
     gene            complement(9992..10837)
                     /locus_tag="VFK00_07040"
     CDS             complement(9992..10837)
                     /locus_tag="VFK00_07040"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592741.1"
                     /translation="MPAAPEPAKPSRAFHFISGLPRSGSTLLAAILKQNPRFHAGMTS
                     PVADIMGVVVAEMSSKNDFSFAISDEARVSVLRGLVENFYAAQSDAEVVFDTSRLWCS
                     RIQMLDTLFPKAKIIACVRPLPWVLDSMERLVRKQPVNVSKVFRFDTNTTIYSRVQAL
                     TDPRGMVGFSFQAIKDAFYGQYAAGHLMLLTYDTLTKNPEAALRAVYDFIGEPWFEHD
                     FDSVEYGASEFDTRIGMPGLHTVRSKVEAPERKPILPLEIFNRFLPESFWLDPKNNVS
                     GVPIV"
     gene            complement(11070..12077)
                     /locus_tag="VFK00_07045"
     CDS             complement(11070..12077)
                     /locus_tag="VFK00_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7592742.1"
                     /translation="MKRANISDVARVAKISRKTVSRVLNNEPNVRESTRQQVLDAMAS
                     LKYSPLTSARSLATNRSFTVGLLYDNRSPSYIMAVQAGVLEACEAQHYSMTVQPLVST
                     SPDFVERVDEILTRHRPDGLILTPPITDHAALLKNLRKSGVPFASISPRHPENQVGVI
                     LREREAAAAMVAHLVSLGHTRIAHIVGDLEHGAGVWRLAGYRDGMKRAGLEEKPAYMV
                     QGRFSFESGVAAARKLLALKQRPTAIFAGDDDMATGTIWAASEAGMSVPGDISVCGFD
                     DTTIATQVWPPLTTIHQPVHAMARRATEELLRLVLGRGKAAMVELDYEMRIRASTGPR
                     P"
     gene            complement(12098..14794)
                     /locus_tag="VFK00_07050"
     CDS             complement(12098..14794)
                     /locus_tag="VFK00_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET7592743.1"
                     /translation="MRTFVHHALVAAAALALVAAPATHAANTFAPRQAGQSVGQVTPI
                     AHWQIQSSAKAQQGGAEISTNGFQAKDWQSVSGRATVMAGLMENGKYKDIFHGDELRT
                     VARHDFVIPWWYRTQFTLTPGASARHVLLRTNGIIGGADIWLNGHKIADHAVVAGAYP
                     VHEFDVTQWIRNGLNTLALRVQPADPQLDFSMGWVDWNPTPPDNNMGPWRGVDVIETG
                     PVELRFPVVTPDLSLPDLARAALTVKVEARNLDSAPHDVVVAGKVAGVPLQQKVHIAA
                     GQTQTVSFSPETDPRLELDHPRVWWPIGMGDQPLYQLKLTASVDGAESDVAAASFGIR
                     SVTSKLTRQGYIQFFVNGKPVLIRGGGWAPDMFLRAPPGRMEAEFSYVRNMGLNAIRS
                     EGKLEDQHFYDLADRYGIMILPGWECCDKWESAAKTGGEPWDDADMEIAQESMASEAR
                     LLRNHPSVIGFLIGSDNAPPPALAKMYVDTLRAADWNLPVVSAAVDQTSTVAGPSGMK
                     MDGPYAWVPPSYWYADKLGGAFGFDSEVSAGADIPRLEDVQRMLSPLFQEALWKFPKV
                     RQFHATESWSPFATLEPFDIALAKRYGPPRDLADYVQKAQLANYDNVRAQFEAFNAHM
                     DSPKPSTGVIYWMLNNAWPSLHWHLYDWYMNPAGAYFGAKKANEALHIQYSYDDHAIL
                     LVNHTLTDAQGLHARVRVRNLDGSVRYDKQFAGVDLAGNHTVKLTTLPELSGLSPTYF
                     VELDLASSDGKPLSRNVYWLSIQQDVLDWKHSNWYLTPVTQYADLTALQGLPAATSNV
                     HATTRREGDDNVTTVTLSIPDSSKAVALFQHLSVHRSADGEAALPILWSDNDITLWPG
                     ESVTLTARYAAKGTAAPVVEVSGWNVPVQHVSAAPAANVDAR"
     gene            complement(14799..16208)
                     /locus_tag="VFK00_07055"
     CDS             complement(14799..16208)
                     /locus_tag="VFK00_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7592744.1"
                     /translation="MPVAETSKRVAESSTPAGGTGFRRSIGLFSGTTINMTQMCGIGP
                     FITIPIMVATMGGPQAIVGWVAGAILAAADGMVWAELGAAMPGSGGTYVYLREAFQYR
                     TGKLMPFLFIWTVLLTIPLIMSTGIIGMVEYLGFFFPHMGWTAAHLVSVLASFLVVFL
                     LYRRIESIRAITAALWAIMLVAVVGTSVAGFSDFHPALAFDFPSNAFGGQFFVGLGAG
                     LIIGIYDYLGYFTTAYMGDELRNPGRTMPGSILISVVAMMLVYLALNISVLGVVPWQE
                     VATSKSIASLVVEHSWGHTAAAVMTILIIVTAFASVFAGLLGGSRVPYQAAQDKVFLS
                     SFGRLHPKYGFPHVALLTMGVVMAAGTFFNLTQVINMLLAASIIVQSAAQIVALVVLR
                     KRQPELPRPYRQWLYPVPCIVAMLGWIYVYVSASTLSLILSGAWIIAGLVVFVVWAKV
                     NHSWPFAPVKIREAYLGDN"
     gene            complement(16454..19651)
                     /locus_tag="VFK00_07060"
     CDS             complement(16454..19651)
                     /locus_tag="VFK00_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592745.1"
                     /translation="MPTIHRRTPLSLAISASLIAMMATSAAASQAPGSQATDPSTQAT
                     AQSATVAGQLQPTDDSNKKTSKAPEAANPATLSVVNVVGVRASQMRAIELKRIAPNIQ
                     DSITAESIGQLPDVTITDALQRVTGVQINRDAGVGSSVDVRGLPEVGTMLNGEVFITP
                     NQIDSQQPDFEMLPATLFRGVDVIKSPTASMLDSGISGDLNLHTYRPWDLPSGFTYSY
                     SAHGQYGDETDKWGKAASGLISYNAGGRWGLLLSGDFSDTTTANSTEGLDQYGVVLNG
                     ENALSADSYYGFLTPWGDAPIPPDIKQNPDGSVDVNGDGQSDGVFMGSQNIALYDTTT
                     QRRRKSGNASFQMDLGGGFTLTSDLFYARQDRYDRTFGIQFNSLDWEGATYVPLQSRN
                     TGASVAGQYGTPDDPPWENTNVYTTQVYKKWPGDVESFSQITRTDSVARNFNVQVDFD
                     NGGPFTATLRGIRDTAHQLNVETDLNIANADGAQWPNDPEDAAPPGTYIYPAELGGNR
                     VFNDNGIPANTVPFTADFTGRNIGISMPDVLADNFANPNGWSMKTLESGGDYDRTVGL
                     SAVRFDGRYDFDNGIKLDFGVRNSIRSAHNLGFTLIAPVYAGMGASDPNGCFVRYVAA
                     DVVMDDPSCSAGNEQGFFRAGPLSAQHLSQTPPPLANNWKEYTDLLGSGITFWAVDPH
                     AMDNPEAYWASLYPGSQRQVTPGRTWAVWLKESSAYLQLDLDGHLGSLPYAANVGMRV
                     IDTNLNVTQHLTGAPAPYGLEPADGGTEVTRRSYRDWLPTANFALDLTDDFKLRLAYS
                     KNMMPLDLGTWGGGLSLNYSLTEVPGQPALFRVAGGNSDGNPNLDPWRSKNFGASLEY
                     YINPSSMVSLAFFRINVQSFIESGSVIDCTLPDEDGVVRDRCVVITEPVQGTGNSIHG
                     AEFDYRQAFTFLPGFLRNTGLEFNATYAPSNSGQTDLAGNKIPFQDNSTKSGNLILWY
                     QDSRLQVRVAYNYRSRRAVSADVGAIAGLEMYEAPQKYIDASVSYKISKYAEVFLQGT
                     NLSNEYQRFYLVWPDQPAHSNFSERMVMAGIRGQF"
     gene            complement(19835..22225)
                     /locus_tag="VFK00_07065"
     CDS             complement(19835..22225)
                     /locus_tag="VFK00_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HET7592746.1"
                     /translation="MTANVAIDPRPGRRWRRPRSNGIHAGLAFVVFATLWVPLAGAKT
                     AASAWSLLPQPAAMHPAGNGVVAVADGATVALSGSQHTQALPIAKQFVGWVGSTRGLH
                     LRLDGSPATHSAIVFDLDPQADVTGDAGYHIAIGDGAIRVVARKPRGLYYGAVTAWQL
                     LTPPGWKPHSTAYVAEGSIDDHPRFAWRGLLLDSGRHFQSVAEIEKLIDWMSLNKLNV
                     LLWHLTEDQGWRLEVPKYPELTSIGACRKAVGLDVELTGSPDKPYCGFYTEAQVREIV
                     RHAAARFVTIVPGIDVPGHSQAAVAAYPWLGVTGKRPEVWTDWGISAWLLKPDEKTLR
                     FLDDVLDEVMRLFPSRYISLGGDEADKTQWNASPEVRARMRSLGLANMDALQGWFTKR
                     LADYLVKHGRTPVGWDDELLAGGTLPVSEVVMTWHGRDKLRVALQAIQQGHDVLLAPV
                     ESLYFDYYQSDLPDEGPGQPGMISLKDAYDTALAPEGATSAEAKHVLGVQAQLWTELV
                     PNFPRAEHALFPRVAALSELGWSPADAHEWDGFLSRMPAEMARYRALGIAYADSAFAP
                     AFSLAPEQDGKLRVTLSNQAGFGAIHYTTDGSPPTAASRRYAAPLEFSLGAGTTVRAA
                     TFAPDGFELSAPRSHVIGSTALLIRDGSQLATCSGRPPMRLAGYRPAHGAAPVYAVDV
                     GNMCWLWKDAPLHGIRQIRVTVGRIAWRFGDDSDGAVVRPAASAAGELQVHGDSCQGT
                     LLATLPLAPAAATKYQTTLGAEVDSGSRAGARTLCIVATGDPRDGQWAIANVSLSR"
     gene            complement(22222..>23016)
                     /locus_tag="VFK00_07070"
     CDS             complement(22222..>23016)
                     /locus_tag="VFK00_07070"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7592747.1"
                     /translation="KVIHDNLPWPITLSRLRKALEFDDVAVLNDFEALGYALDEIRSA
                     GGELLCGPDTCAKGPTLVVGPGTGLGAAVRLAGRARSVVLTTEAGQMDFAPQTIRERE
                     VLRSVSPGGGYVPYESIVSGPGLLTLYRTLCGLNGDPPALATPEAITAAARARVDRQA
                     VEAVGMFCDVLGSFVGNLAMVFMARGGVYLAGGFLSSIFDLLRQSRFAERFLHERSVR
                     EFLSRVPVRVIEHGQHGLLGAARWYLDQLSHDRPEDERVAACGVAG"
BASE COUNT         3984 a         7668 c         7497 g         3867 t
ORIGIN      
        1 cgctgcgcca tcgacacctc gtccggggaa ggcgtcgagg ccgccgcgcg acgcgcacgc
       61 tacgccgccc tcgccggttc attgcgggac ggcgaatggc tggcgctggc ccagcaccgc
      121 gacgaccaga tcgaaacggt cttgctgaag ctgctgcgcg gcgcgggacc ggaaggactg
      181 ggcggcatgc gtcccacacg cccgctgggt cccggcctcc tgtggcgacc gttgctcgaa
      241 accaatcgag acgagctcgc cagatatttg attcagcacg aaattgcgag cctcgatgat
      301 cccgggaacc aggatccgcg gtttgcgcgg aacctgctgc gccacgagat cctgccggcg
      361 ataaagcgcc acttgccgca cgcgggttcc gccatcctgc acagtgcccg cctgtgccgc
      421 gttgccagcg atcatctggc acgcgacgcc gacggcgccc tcgcgcgagt gcgccgcgac
      481 gacgcgaccc tggatgccga tgcatggcac gcgctccccg aagccgtgcg cgcgctggtg
      541 ctcgaccgct ggctgcgcga acagggcctg cccgctccgg cggatgcgca gcgcctggaa
      601 ctcgagcgcc aggtcgccgg tgctgccgaa gatgccgggc cctgcgtcgg ttgggacggc
      661 gccgaggtgc gcatctggga cggtcgcctg catgcctcgc ggccgctacc gccgttgccc
      721 ggcgactggc gttcggcgtg ggagggcggg atcctcgcgc tgccgaaagg ctgcggcacc
      781 ctcgtgaccg atggagggga accccgatcg gcaccgacct tcgacccgcc gctcgaagtc
      841 cgcctgcgtc gcggcggcga gcgcatcaag ccggctggcg atccgcacac ccgcgaattg
      901 cgcgcgctct tccagcaggc gcggattccg ccgtggctgc gcgtacgctg tccgctgatc
      961 tacgccggcg atgaactggt cgccgtcgcc gaccgctgga ccagcgcgca cggcaacgcc
     1021 atcttcgatg cctgcaacgt acggccacgc tggaagcgac ccgcgatcgg gtgaagaatg
     1081 gtacgttcgc gccatgggcc gctcccgcat aagcgcgatc gatttggaac gccatccgcg
     1141 gcgcgaccgc tcggggccaa ccgcgaatcc ccgctcattc ggcggcactc cgatcgtgat
     1201 cccgccccac gatgatgcgc gcgttgcgtt gccaggtcag gtacgaccac gcccagttcc
     1261 acatcacggc gaagcggttg cggaagccga tcaggaacag gatgtgggcg aacagccaga
     1321 acagccaccc ggtgaagccg ctcatctgca ccccgcgcag gtcgaccacc gccgatgcgc
     1381 ggctgacgct cgcgaggctg ccgtaatcgc gataacggaa cggccgcgac gaaccgccat
     1441 cgaggcggtc gcggatcacg cacgctgcgt gcgaccccat ctgcttcgcc gccgacgcca
     1501 tgcccggcac gagcttgccg tcctgttcca cgtgcgcgag gtcgccgatc accaacactt
     1561 cgggatggcc gggcagcgac aggtccggcg ccaccatcac gcgcccgtgg cggtccagct
     1621 cggaagcgag cttcgtaccg agcctcgatg ccgcaacacc ggcggcccac aacagcgtac
     1681 gtgcggcgat gcgttgatcg cccatccgca cgccggtttc gtcgacctcc gtcaccgcgg
     1741 catcgacatg cacccgcaca cccatgctct cgagtttgcg gcgcgccgcg gccgacagcc
     1801 gctccggcat cgcgccgagc acgcgcggcc cggcttcgac caggtggatc gtcgcgtggc
     1861 ggggatcggc gtggcggaat tcatgcgcca acgtgtgctg cgcgatttcg gccagggtgc
     1921 cggcgagctc cacgcccgtc ggcccggcgc cgatgatcac gaagctcagc cactcggcgc
     1981 gtcgctcggg atcgggctcg cgctcggcaa tctcgaacgc cgaaaggatt cggcgccgaa
     2041 tcgccagagc gtcgtccatg gtcttgaggc ccggcgcata cggctcccag tcgtcatgtc
     2101 caaagtaggc gtgcgtggaa ccggtcgcga cgacgagcca gtcatattcg taatctcccg
     2161 caccggtgcg gacgctgcgt ccggccacgt cgatgccctg cacctcttcc atcagcaccg
     2221 tgacgttgtc ctggtggcgc acgatgtggc gcagcggcgc ggcgatgtcc gggcccgaca
     2281 gactggcggc cgcgacctgg tacagcagcg gctggaacac gtggtggttg gcgcggtcga
     2341 tgagcgttac gcgcaccggc acgcgggcca gcgcccgtgt ggcccaaagg ccgccgaagc
     2401 cgccgcccag gatcaccacg tggggccgtc gcgcgggatc gtccatgcgg gcatgctagg
     2461 gaacggcagg tgacctcaac atgaccgttg gcaccttgca cccatggccg gcccgttatg
     2521 caataccgga tcggcctggg cggagggatt tgcgatgcaa cgacgtcact cgtgggcttt
     2581 gctcgccttg ctgttgctgg ccggcgccgc gccggccgcc cattccgcgg cgcccgcgcc
     2641 ggatgcgcaa ggctttactc ttcagcaggt gctggcctat ccgtacccgg agggtctggc
     2701 cgcctcgaag gccggcaacc gcattgcgtg ggtgatcgac cagaatggcg tgcggaacgt
     2761 ctgggtcgcg cagggccccg cattcgagcc gcaacaggtc acgcgtttca cgaacgacga
     2821 cgggcaggaa atcacccagc tcactttctc gccggatggc aacgacctgg tgttcgtgcg
     2881 cggcggcgac cacgacgcca attggcccga aaagctgccg cccgaccctg ccgccaaccc
     2941 ggccgagccg aaggtcatgc tgtgggtggt ggacctcggc gacgggactt cgcgcgcgct
     3001 gaccgaaggc gacgaaccgg cgatctcctc gcagggcaag ctcgcgtaca ccagggacca
     3061 tcaggtctgg accgcgtcgc tcgccggggg cgaccacgcg aaacccaaac gcctgttctt
     3121 cgaccgcggc aaggacggcg acctgcaatg gtcaccggac ggcaagcggc tggccttcgt
     3181 gtccgaccgc ggcgaccatg ctttcatcgg cgtctacacg gacggcgacg cgccactcga
     3241 atatctcgcg ccctcgaccc acaacgacgg catgccgcgc tggtcgccgg acggtacgcg
     3301 cgtcgccttc acccgccgcg atggcgatgg cggcccgccc aagcccatcc tcgagcagac
     3361 gcccgaaccg tgggcgatct gggtggcgga tgccggcacc ggcgaggcgc acgcgctgtg
     3421 gcaaagcccg gacactgcct tcggctcgta cccggaagcc ccgggcggcg ccaacctgca
     3481 ctgggccgat ggcggtgcgc ggctggtgtt cctcgcggat ctggacggct ggccgcacct
     3541 gtactcgatc actgcaaacg gcggcacgcc gctgctgctg acgccgggca agttcatggt
     3601 cgaggatgtg gtgctgtcgc gcgacggccg ttcgctgatc tacaacgcca atgccggcga
     3661 aagcgcaggc gacgtcgacc ggcgccactt gttcaaggtg cccgtcgatg cggccaaacc
     3721 ggtgcctctg accagcggag ataacctcga gtgggccccg accgccgccg acgcaaacac
     3781 gatcgcatac gtcgaagccg gttcgcggaa accgccgatg ctggcagtga tgcacatcga
     3841 cggcacggga caaaggctgc tgcaacccga gctgctaccc gaggcgttcc cgtcggggca
     3901 actggtggtg cccaagccgg tgacattcac cgccgccgac ggcaccctga tccacggaca
     3961 gctcttccgc agcacggatg ccagcgcgga gcagccgggc gtgatcttcg tgcacggcgg
     4021 cccgccgcgg cagatgctgc tcggttggca ttacatgggc tactacagca acagctacgc
     4081 ggtgaaccag tacctcgcca cccacggctt cacggtgctg tcggtcaact accggctcgg
     4141 gatcggccac ggccacgcct tccaccatcc cgcgcactgg ggtccgaccg gcgcctcgga
     4201 ataccaggat gttgtcgccg gcgcgaagta cctgcagcac gtgccgggcg tcgatgcctc
     4261 gcgcatcggc atctggggcg gctcctacgg cggctatctc accgccctgg cgctggcccg
     4321 caattcggac atcttcaagg ccggcgtgga catgcacggc atccacgact ggtcgcgcga
     4381 catcgccgaa tggttcgaca aacccgacgg acgctacgaa aagggcgact accagcaggc
     4441 gatgaaagtc gcctgggaat cctcgcccga cgcctcgatc gccgaatgga cgtccccggt
     4501 gctcctgatc cagggggacg acgaccgcaa cgtccatttc ctgcagatgg aagacgtggt
     4561 cccgcgcctg cggaaacacc atgtcccctt cgaggaaatg gtgctcccca acgagatcca
     4621 cgggttcctg cgccatgcgt cctggttgaa agccgacacc gcgacagtgg atttcctgga
     4681 acgcaaactc gatgcaaacg gagagcattg atgaaccccg ctcctgcggg agaggcggcc
     4741 gtggaccggg cgcgcagcgc tcgtgaagcg ccgggtgcag attcggaaat gatcgacctc
     4801 gacgcatatc tgcggcgcat cggcctccaa acttccgtcg cgccgaaccc gcaaacattg
     4861 cgcgccatag ttgccgccca cgtcgcggca atccccttcg aaaacctcga ccccttcctc
     4921 ggcattacgc cgtcgcttga catcgcatcg gtgcagcaga aactggtgcg taatggccgc
     4981 ggcggctact gcttcgaaca caaccgcctg ctcagcgacg ctctgcgtgc gatcggcttc
     5041 gacgtcaccg acctcggcgc ccgcgtgcta tgggggcaat ccgaagacgc gatcaccgcg
     5101 cgcagccaca tgctgctgcg cgtcgagatc gaagggcgtt catggttggc cgacgccggc
     5161 ttcgggggca atacgcctac agccccgctg gaactcgttc ccgatatcga acaggccacg
     5221 ccgcacgaac cctaccggct gatccgccgc agcgacagcg actggcggtt gcaacttttc
     5281 gcggcggacg cgtggcagac gctgtaccgc ttcgacctgc agccgcaatt tcccgccgat
     5341 taccgcgtca gcaactactg gacatccacg aaccccgatt cgcacttcgt ctcgggcctc
     5401 accgccgccc gcgcgcccgc cggccgcagg ctcgccctgc gcaaccgcca gttcaccgag
     5461 tacacgaccg gcggtggcac caccaggcga acacttgcca ccaccgcgga aatccgcgcg
     5521 atactccagg acgaattcct gattcccctg cccgagcgtc caaagctggg ggaaatgctg
     5581 gatcgccttc cggaatgact gccgccggtc tcgaagcgcc ggccgacgcg cggctgcgga
     5641 cctcgccggc cttccaggga cagactccat gccccgtaac ccctaccgtc tgatcgcggc
     5701 ggtcgcctgg aggccagcga tcttgcaccg cggcacccga ctgttcacac tgcgaccagc
     5761 ggaggccggc cggttgccgg cgggatgacc tggggcgcta ggtggccgtt gcacgccgac
     5821 tgttcgccga actcaggcgc cgcaacgtac tgcgcgcggc ggtgttgtac gtcggcgcgg
     5881 tgtgggcgct cgcgcagggc gtcgcgcaat tgggccccac cttcggcgcg cccgactgga
     5941 ccacgcgcgc tttcgtcatc gccgctgcgg tcggctttcc attctggatc gcgttcgcct
     6001 ggatcttcga gttcacgccg cagggcctca agcgcgagag cgaagtcacc gaggacgaat
     6061 cactggcgca ctgggccggc cgcaggctgg atttcgccat catcgccgtg ctgtcggtcg
     6121 cggtagccct tcttctcggc aaccagttcc tctggcacaa gggcgctgcc caggggccgg
     6181 ccgcatcggt cggggcagcg gcgaagattc cgcagaagtc cgtcgcggtg ttgccgttcg
     6241 ccaacgagag cggcaaagca ggcgaacaat tcttctccga cggcctgtcg gaagacctga
     6301 tcacggcgct ttcgcaggtc gacggcctga aggtgatcag ccgcaattcg gcgttccagt
     6361 tccgcgacag caaggaaaat gcgaaggcca tcggcgggaa actcggtgtc gcgcacctgc
     6421 tggaaggcag cgtgcaacat gtcgaggagg cagtgcgcat caccgccacg ctggtcaaca
     6481 cggccgacgg cagcatcgtg tggtcacagc gctacgacaa gccgtaccgg gacctgttct
     6541 cgttgcagga cgagatcacc ggagccgtgg ccagtgccct gaaggccagg ctgctgagcg
     6601 cacgcggcgc ggtggtgcaa agcgatcgcc cgcccagcgg cagcctcgcc gcctacgcgg
     6661 cctacaagca cgggcgtgcc tacggcgcgc tcggcaccga agccgacttg cgtcgcacca
     6721 tcgacgcgtt cgacgaggcg accaggctcg atccgcacta cgcggcggcg tatgcgcggc
     6781 tgtccctggc gtggaatgcg ctcgccgcgc aggcgctggg aggcaaggcg gccgccgagg
     6841 ccaacgcgca agcgcgcaat gcggtgaaca ccgcattgag cctcgacccg aattccgcgc
     6901 tcgcacatcg ggcgcacgca tacctgctcc tcaacgtgga catggactgg acggccgtcg
     6961 aggccgagac ccggcgcgca ctgcaactgg cgcccaacga cgcagtggca cgattcgacc
     7021 acggcgtcgc cttggcgtcg ctggggcagg tgcaagcggc agtgacgctg accaggcaat
     7081 ccctggacac cgatccgcgg gacgatgatt cgttttattg gctggcctct tatctcgccg
     7141 gattgggccg cctggacgaa gcgcgggagg cgatcaacac cgcgatcgcc ttgcaacctc
     7201 gcgcggacgg ctatcgcgcg ctgctcgcga ccatcgaagt gctgcgcggc gatgccaagg
     7261 cggcgagggt cgccgcgaag caggaatcac cgggaggctt gtggcacgac accgccgtgg
     7321 caatggcatc gcaaatcggc ccggatcgcc atctggcgga tgcggtgttc gacaagctgg
     7381 tcgccgggca cgccgaagac ggcgcgtacc agattgcgca gatccaggcg ctgcggcgcg
     7441 atgccgacca gatgttccag tggctggaac gtgcctggac gaaccgcgat ccgggcgtcg
     7501 gatacctgct ctcggatccc ttcctcctgc gcttccagca cgacccacgc ttcgccgcgt
     7561 tctgcaagaa ggtcggcctg ccgaccacga ccgatgcgaa ggcgatgccc tgagcgcgag
     7621 ccgccctccc ggcgtgggtg ctgcagcagt ctcgatcatg cagggcccat gcgtcagcgg
     7681 cggccggctt cttgacacgc gtgtgccaca ttgacatatt tatgccatgg ccgcaaaggc
     7741 aagcccgctt cgccagcgac aagcgcagca ggtgcgtgtg gcactgctcg aggcggcgat
     7801 tcgtcagttg gaaggcacag gtgacgtatt gatggccgag gtcgctgaag cagccggggt
     7861 ttcactccgg acgctttacc gctacttccc cgatcgggcg tcgttgctgg gtgccgcggg
     7921 cgaccacctg tatcgctcgc tcggcgttcc gttcgggatc gcggggccgg acaatatcgc
     7981 ggccagcttt cgcgaagctg cgcgccggct tggcactcgg cccctgcttg cgcgtgcgct
     8041 cgtgcggagc gaggccggga cactctcccg atccagtgtc cgccgagacc ggcttgaagc
     8101 cattcgtgat gccctcgagc cgttgaccga agccctcgat ccatgcaccg cccagcgcgc
     8161 gtccgccgtg atcgcccacc tgtgcagcgc ctcatcatgg gtgggcatcg ccgacgaggg
     8221 cggcctcagc gactcggatg cccatgagac cgtggcctgg gccatcgata cgttggtggc
     8281 tacgctccgc aaagaggctc gccgctcgcg accccaccgc aaccgccaac aggcaccacg
     8341 aaaccggagg accaaccatg agccagactg accacctctt gcacgattgc ggcaacggag
     8401 acctttacac cgagccggaa aggagccggg cctggtggtt cctgggcacc cttgccgtgc
     8461 tccggaaccc gcgcggcgcg ccgacgtgcc cgacgatcat cgagttgacc gttccgccag
     8521 gcggttcgcc gccgcggcac atccacgagt cgctggatga caatttcctg cttctggacg
     8581 gacaggtcgt cgtccgatgc ggcgagcaaa cgttcgtcgc gcggcccggg acttacgtaa
     8641 cgctcccgca cggcgtggac cacactttcc gcgtgaccag tgccctgccc gcccgcatgc
     8701 tcctcctgca cgcgagcgac gagtttctgc gattcatcga ggccgcgggt actccgaccc
     8761 atgaacggcg gctaccgccc cctggtgagc acgacgtgag ccgggatgtg ctcatccgcc
     8821 tcgccggcga acatggcgtc cggatcatcg gcgctcccat ggcagatgac gagacgcgaa
     8881 ggctcgtcga atcagcgtag gcgctttgcc tgctggtcgc cggagccagc tgcttgctga
     8941 tcgcaaagca cctccggttc tgtgcttccg atggaatcaa tcgctgaagt tggagtgcag
     9001 gtagcaggtc agcgctacgg cacaaaccct gctgacaggg aaagccatga ccgacgctac
     9061 aagcgaatat ctcgcgggct gccactgcgg cgcactcacc gcgcgattcc gaacggcgct
     9121 ggcgacatcg gcatggcagg tgcgcgggtg ccagtgcagc ttctgtcgtg cgcatgctgg
     9181 tctgaccatc tccgatccat ccggaagcct cgagttcgcc gctgtccacc ccgagcaagt
     9241 ccaacgctat cgctttggtc gtcgtgtaac cgactttctt ctctgccgca cctgcggcat
     9301 ctacgttggt gcgtgcgttg ccgggccaaa tggccgattc ggcgtcctga atgcgcatgc
     9361 gttgcggccg attccggcga accttccgca gccggtgccg atggactacg ccgcagaaac
     9421 cctgcttgaa aaacaacagc ggcgtgaagc gcgatggacg ccgctcacgg catcgagcct
     9481 gtgagaaggc aaagactggt cgcgaccccc aagccgacat cgtgaccatc cgcacccggc
     9541 cggccggtaa ccgacgagcc tcgaccgaaa tcggacattc gcggggactg cggtgaaatg
     9601 atgaagcacg ctgctcgcac gccgcccgac ttcgcttttt gcttgtcgtt cggcgacatg
     9661 tacacacgct tcggctggcg catctgcaga acgcggttga agccactcgc ctcgctggat
     9721 gggggtaaag tggcgtgctc gaggaacgac gcgcagaacg acagcgcggg atcggtgcca
     9781 agctcggcgt agcgaccctg ctccaaggca ccgtgcgcag acagggcgac caggcgcgca
     9841 tcgtggtatc cctgatcaag cctccgatgg cagccagatc tggtcatgct gatcgaatga
     9901 cggatatcgg gaaattgaag atcggacgtg accgaccggc catgccccgt ttttctgccg
     9961 gttgcaccga aacaaaaccc gccctcgacc ctcacacgat gggcacaccg ctgacgttgt
    10021 tcttggggtc gagccagaac gactccggca ggaagcggtt gaagatttcg agcggcagga
    10081 tcggtttgcg ctccggggct tcgaccttgg agcggaccgt gtgcagcccg ggcatgccga
    10141 tacgcgtgtc gaattcgctg gcgccgtatt cgacactgtc gaaatcgtgc tcgaaccatg
    10201 gttcgccgat gaagtcgtag accgcgcgca gcgccgcttc cggattcttc gtcagggtgt
    10261 cgtaggtcag gagcatcagg tggccggcgg cgtactggcc gtaaaacgcg tccttgatgg
    10321 cctggaagga aaagcccacc atgccgcgcg gatcggtgag cgcctgcacc cgcgagtaaa
    10381 tagtcgtatt ggtatcgaac cggaacacct tgctgacgtt gaccggctgc ttgcgcacga
    10441 ggcgctccat gctgtccagc acccacggca atggtcgcac gcaggcgatg atcttcgcct
    10501 tcgggaacaa cgtgtcgagc atctggatcc ggctgcacca caggcggctg gtgtcgaaca
    10561 ccacttcggc atcgctctgg gctgcataga agttctcgac cagtccgcgg aggacggaga
    10621 cccgagcttc gtcggaaatc gcgaatgaaa aatcgttctt gctgctcatc tccgcgacca
    10681 cgacgcccat gatgtcggcg accgggctgg tcatgccggc atggaatcgc gggttctgct
    10741 tcaggatcgc cgccagcaac gtgctgccgg agcgcggcag cccggaaatg aaatgaaacg
    10801 ccctggatgg cttggctggt tccggtgcgg ctggcatgcg tgcgctcctc gtggtcggtg
    10861 tgctttgccg ggccagtatc gcaggcgcgg cacgcggatt tttgtacgtc gcaggttgcg
    10921 attgttgggc ttcaccggca ggagcagccg gttcaacccg acctaccgcc agctctatcg
    10981 gcggaaggcc aacgtcgtcc tgacgttggg cttcacccgg agcatgctcc gggttcaacc
    11041 caacctacga tcgcgcgaac gggtggcgcc tatggccgtg ggcccgtcga agcgcggatc
    11101 cgcatttcgt agtcgagttc gaccatggcc gccttgcccc tgccaagcac gagccgcaac
    11161 agttcttcgg tggcgcgccg cgccatggcg tgcacgggct ggtggatggt ggtcaacggc
    11221 ggccacacct gggtggcgat ggtggtgtcg tcgaagccgc acacggaaat gtcgccaggc
    11281 accgacatgc cagcctccga tgcggcccag atcgtgccgg tggccatgtc gtcgtcgccc
    11341 gcgaagatgg cggtgggtcg ttgcttcagc gccagcaatt tgcgcgccgc ggcgacgccc
    11401 gattcgaacg agaaccggcc ttgcaccatg taggccggct tctcctcgag gccggcgcgc
    11461 ttcatgccgt cgcggtagcc ggccaggcgc caaacgccgg cgccgtgctc aaggtcgccc
    11521 acgatgtgcg cgatgcgcgt gtggcccagc gaaaccaggt gcgccaccat cgcggccgca
    11581 gcctcgcgct cgcgcaggat aacgccgacc tggttttcgg gatgccgtgg ggagatcgag
    11641 gcgaacggca ccccgctctt gcgcaggttc ttcagcaagg ccgcgtgatc ggtgatcggc
    11701 ggcgtgagga tcaaaccgtc gggccggtgc cgcgtcagga tctcgtccac ccgttcgacg
    11761 aaatcgggtg aagtcgaaac gagcggctgc accgtcatgc tgtagtgctg cgcttcgcag
    11821 gcttccagca cgccggcctg cacggccatg atgtagctgg gtgaccggtt gtcgtacagc
    11881 aggccgaccg tgaaggagcg attggtcgcg aggctgcgcg ccgaggtgag cgggctatac
    11941 ttgagggagg ccatcgcatc cagcacctgc tggcgcgtgg actcgcgcac gttgggctcg
    12001 ttgttgagca cccgcgaaac ggttttccgc gagattttcg caacacgggc cacgtcgctg
    12061 atgttggcgc gcttcatggg cgtcgttggc aatggtttca gcgtgcgtcc acgttcgccg
    12121 cgggcgcggc cgacacgtgc tggaccggga cattccagcc gctcacctcg accaccggcg
    12181 ccgcggttcc cttcgccgcg tagcgcgcgg tcagcgtcac cgactcaccg ggccacaggg
    12241 tgatgtcgtt gtcgctccac aggatcggca acgccgcctc gccatcagcg gatcggtgga
    12301 ccgacaggtg ttggaacaaa gccaccgcct tcgacgagtc cggaatcgag agcgtgacgg
    12361 tggtgacatt gtcgtcgccc tcgcgccgcg tcgtggcgtg cacgttgctg gtggcggcgg
    12421 gcaagccttg caaggcggtc aggtcggcgt actgcgtgac cggcgtcagg taccagttcg
    12481 aatgtttcca gtcgagcacg tcctgctgga tcgagagcca atagacgttg cggctgagcg
    12541 gtttgccatc gctcgaggcc aggtcgagtt cgacgaaata cgtcggcgac aatccggaga
    12601 gctcgggtag cgtcgtcagt ttcaccgtgt ggttgccggc gagatcgacg cctgcgaatt
    12661 gcttgtcgta gcggacgctg ccatcgaggt tccgcacccg cacccttgcg tgcaggccct
    12721 gcgcatcggt gagggtatgg ttcaccagca agattgcatg gtcgtcgtac gagtactgga
    12781 tgtgcagcgc ctcgttggcc ttcttggcgc cgaagtacgc gcccgccgga ttcatgtacc
    12841 agtcgtacag gtgccaatgc agcgacggcc acgcgttgtt gagcatccag tagatcaccc
    12901 ccgtcgaagg cttgggcgaa tccatgtgtg cgttgaaggc ttcgaactgt gcgcggacat
    12961 tgtcgtagtt ggcaagctgc gccttctgca cgtaatccgc gaggtccctg ggcggtccgt
    13021 agcgcttcgc cagtgcgatg tcgaacggct ccagcgttgc gaacggcgac cacgactccg
    13081 tcgcgtggaa ttgcctgacc ttcgggaact tccagagcgc ctcctggaac agcggtgaca
    13141 gcatgcgctg gacgtcttcc aggcgtggga tgtcggcccc cgcgctcacc tcggaatcga
    13201 acccgaacgc cccacccagc ttgtcggcgt accagtacga cggcggcacc cacgcatacg
    13261 gcccgtccat tttcatgccc gaaggaccgg ccaccgtgct ggtctgatcg acggcggcag
    13321 agacgaccgg caggttccag tccgccgcgc gcaaggtatc gacgtacatc ttggcgaggg
    13381 ctggcggcgg cgcgttgtcg ctgccgatca ggaaaccgat gaccgacgga tggttccgca
    13441 gcagtcgcgc ctcgctcgcc atggattcct gcgcgatctc catgtccgcg tcgtcccatg
    13501 gctcgccgcc cgtcttcgcc gccgactccc atttgtcgca gcattcccag ccgggcagga
    13561 tcatgatgcc gtagcgatcg gcgaggtcat agaaatgctg gtcctcgagc ttgccttcgc
    13621 tgcgtatcgc gttgagcccc atgttgcgga cgtaactgaa ttcagcctcc atgcgtcccg
    13681 gcggcgcacg caggaacatg tcgggtgccc agccgccgcc gcggatcagc accggcttgc
    13741 cattgacgaa gaactggatg tagccctgcc tcgtgagctt cgaggtaaca ctgcggatgc
    13801 cgaagctcgc cgctgccacg tcggactccg caccatccac ggaagcggtg agcttcaact
    13861 ggtacagcgg ctggtcgccc attccgatcg gccaccagac cctgggatga tccagctcga
    13921 gccgcgggtc ggtttcgggc gaaaatgaaa cggtttgcgt ctgccccgca gcgatatgga
    13981 ccttctgctg caaaggcacg ccggcgactt tcccggcgac gacgacatcg tgcggcgcgc
    14041 tgtcgagatt ccgcgcctcc accttcacgg tgagtgccgc gcgcgcaagg tccggaagcg
    14101 aaaggtccgg cgtgaccacg gggaaacgaa gctcgaccgg cccggtctcg atgacgtcaa
    14161 cgccacgcca cggccccatg ttgttgtcgg gcggtgtcgg attccagtca acccagccca
    14221 tcgagaaatc gagctgcgga tcggccggct gaacccgcaa cgcgagcgtg ttcaagccgt
    14281 tccggatcca ttgcgtgaca tcgaactcgt gcaccggata cgcgcccgcg acaacggcgt
    14341 gatccgcgat cttgtgccca ttgagccaaa tgtccgcgcc accgatgatt ccgttggtgc
    14401 gcaacaacac gtgtcgggcg cttgcgcccg gcgtgagcgt gaactgcgtg cggtaccacc
    14461 acggaatcac gaaatcatgg cgggctaccg tgcggagctc gtctccgtgg aatatgtcct
    14521 tgtacttgcc gttttccatc agcccggcca tcaccgttgc gcggccgctg accgactgcc
    14581 agtccttcgc ctggaaaccg ttggtcgaaa tctcggcccc gccctgctgc gccttggcgc
    14641 tcgactggat ttgccaatgg gcgatcgggg tgacctggcc gacactttgc cctgcctgcc
    14701 ttggcgcgaa cgtgttggcg gcatgcgtgg caggtgccgc gaccagcgcg agcgcagccg
    14761 cagccaccaa agcatgatgg acgaaagttc gcatggcgtc agttgtcccc cagataggct
    14821 tcccggatct tgacgggcgc gaacggccag gaatggttga ccttcgccca caccacgaac
    14881 accaccaggc cggcgatgat ccacgcgccg gacaggatca gcgacaacgt cgaagccgac
    14941 acgtacacgt agatccatcc gagcatcgcg acgatgcacg gcaccgggta cagccactgc
    15001 cgatacggcc gcggcaattc cggctggcgc ttgcgcagta ccaccagcgc cacgatctgc
    15061 gcggccgact gcacgatgat gctggcagcc agcagcatgt tgatcacctg cgtcagattg
    15121 aagaaggtgc ccgccgccat cacgacgccc atcgtcagca aggccacgtg cgggaagccg
    15181 tacttcggat gcagccgtcc gaacgaggac aggaacacct tgtcctgcgc cgcctggtaa
    15241 ggcacgcggg agcctcccag caggccggcg aataccgatg cgaacgcggt cacgatgatc
    15301 aggatcgtca tcaccgccgc cgcggtgtgc ccccaactgt gctcgacgac cagcgaggcg
    15361 atcgacttcg aggtcgcaac ctcctgccac ggcacgacgc ccaggacgct gatgttgagg
    15421 gcgaggtaca ccagcatcat cgccaccacc gagatcagga tggaacccgg catcgtccgg
    15481 cccggattgc gaagttcatc gcccatgtac gcggtcgtga agtagccgag atagtcgtag
    15541 atgccgatga tcaggccggc gccgagtccc acgaagaact ggccgccgaa ggcgttgctc
    15601 ggaaagtcga aggccagcgc gggatggaaa tcggagaacc ctgccactga tgtcccgacg
    15661 accgcgacca gcatgatggc ccacaacgcc gccgtgatcg cgcggatgga ttcgatccgg
    15721 cggtacaaca ggaaaacgac gaggaagctc gcgagcacgc tgacgaggtg cgcggcggtc
    15781 cagcccatgt gcgggaaaaa gaagccgagg tattcgacca tgccgatgat gccggtggac
    15841 atgatcaacg ggatcgtcag cagcaccgtc caaatgaaaa ggaacggcat cagcttgccg
    15901 gtacggtact ggaaggcctc gcgcaggtac acgtaggttc cacccgaccc cggcatcgcc
    15961 gcacccagtt cggcccagac catgccatcg gccgcggcga ggatggcacc ggcgacccag
    16021 ccgacgatgg cctgcggtcc gcccatggtc gccaccatga tcgggatcgt gatgaacggc
    16081 ccgatgccgc acatctgggt catgttgatc gtggtgccgg agaacaggcc gattgaccgg
    16141 cgaaagcccg tgccgcccgc gggcgtgctg ctttccgcta ctcgtttgct ggtttccgct
    16201 actggcatgt cgtttccgga tggatgttgg aatgcgccgg gcgagcgctg catcaggtca
    16261 cacttgctgc atacccgaag caatgtcccg aaatgtattc gtcattccgg acgaatccac
    16321 gaagtggatg ggacccggag cctgctcctc atcgcgcaag accggagcag attccggatt
    16381 ctcgcgccgg caaggccggc gcaagccccg gaacgacgat gtgtctcgat ggttcatcac
    16441 acccaggaac tcctcagaac tgcccgcgaa tgccggccat gaccatgcgc tccgagaagt
    16501 tcgaatgcgc cggctggtcg ggccacacca ggtaaaaacg ctggtactcg ttgctgaggt
    16561 tggtgccttg caggaaaact tccgcgtact tgctgatctt gtacgagacc gacgcgtcga
    16621 tgtacttctg cggcgcctcg tacatttcca ggccggcgat ggcgccgacg tcggcggaaa
    16681 cggcgcggcg cgaacgatag ttgtaggcga cgcgcacctg cagccggctg tcctggtacc
    16741 aaaggatcag gttccccgac ttggtcgaat tgtcctggaa cggaatcttg ttgccggcaa
    16801 ggtccgtctg tcccgagttg ctgggggcgt aggtggcgtt gaactccagg cccgtgttgc
    16861 gcaggaagcc gggcaggaag gtgaacgcct ggcgataatc gaactcggcg ccatggatgc
    16921 tgttgccggt gccctgcacg ggttcggtga tcacaacgca acggtcgcgc accacgccgt
    16981 cctcatccgg cagcgtgcag tcgatcacgc tgccgctttc gatgaagctc tgcacgttga
    17041 tccggaagaa cgcgaggctc accatgcttg aaggattgat gtagtactcg agcgatgcgc
    17101 caaagttctt ggagcgccac ggatcgagat tggggttgcc atccgaattg ccgcccgcga
    17161 cgcggaacag cgcgggctgc ccgggaacct cggtcagcga ataattcagc gaaagcccac
    17221 cgccccaggt gccgaggtcg agcggcatca tgttcttcga ataggccagc cggagcttga
    17281 agtcatcggt gaggtcgagc gcgaaattgg cggtcggcaa ccagtcccgg taactgcgcc
    17341 gcgtgacctc ggtgccgccg tcggccggtt ccaggccgta cggggcgggc gcgccggtga
    17401 ggtgctgggt gacattgaga ttggtgtcga tcacgcgcat gccgacgttg gcggcgtatg
    17461 gcaggctgcc gagatggccg tccaggtcga gttgcaggta ggccgagctc tccttcagcc
    17521 acaccgccca cgtccgcccc ggcgtcacct ggcgttggct gcctggatac aacgacgccc
    17581 aataggcttc gggattgtcc atcgcgtgcg ggtcgaccgc ccagaaggtg atcccggatc
    17641 cgagcaggtc cgtgtattcc ttccagttgt tcgcaagcgg tggcggcgtc tgcgaaaggt
    17701 gctgtgcgga caacggtccc gcgcggaaga aaccctgctc gttgccggcc gagcaactcg
    17761 gatcatccat caccacgtcg gcggcgacgt aacgcacgaa gcatccattc gggtcgctgg
    17821 cgcccatgcc ggcgtacacg ggggcaatca gcgtgaaccc gaggttgtgc gcgctgcgga
    17881 tgctgttgcg caccccgaag tcgagcttga tgccgttgtc gaagtcgtaa cggccatcga
    17941 agcgaaccgc actcagtccc accgttcggt cgtagtcgcc gccggattcc agcgtcttca
    18001 tggaccagcc gttcgggttc gcgaagttgt ccgcgagcac atcgggcatg ctgatgccga
    18061 tattgcggcc ggtgaaatcg gcggtgaacg gaaccgtgtt cgcgggaatg ccgttgtcgt
    18121 tgaacacgcg gttgccgccg agctccgcgg gatagatgta cgtgccggga ggcgccgcat
    18181 cctcgggatc gttgggccac tgggccccgt cggcattggc gatgttgagg tcggtctcga
    18241 cgttcaactg gtgcgccgtg tcgcggatgc cccgcaaggt cgcggtgaag gggccgccat
    18301 tgtcgaaatc gacctggaca ttgaagttcc tcgcgacgga atccgtccgc gtgatctgcg
    18361 aaaacgattc gacgtcgccc ggccacttct tgtagacctg cgtggtgtac acgttcgtgt
    18421 tttcccacgg cgggtcatcg ggcgtgccgt actggccggc gacactggcg ccggtattgc
    18481 gcgattgcag cggtacgtag gtcgcgcctt cccagtcgag cgaattgaac tggatgccga
    18541 aagtgcggtc gtagcggtcc tggcgggcat agaagagatc gctggtgagg gtgaacccgc
    18601 caccgaggtc catctggaac gaagcgttgc cggacttgcg cctgcgctgg gtggtggtgt
    18661 cgtacagcgc gatgttctgg ctgcccatga acacgccatc ggattgcccg tcgccgttga
    18721 catcgacgct gccgtcggga ttctgcttga tgtcgggcgg aatcggcgca tcgccccagg
    18781 gcgtgagaaa gccgtaatag ctgtccgcgc tgagtgcatt ctcgccgttc agcacgacgc
    18841 cgtactggtc gaggccctcc gtcgagttgg cggtggtggt gtcggagaaa tcccccgaca
    18901 acaacaagcc ccaacgtccc ccggcgttgt aggagatgag gccgctggcg gccttccccc
    18961 atttgtcggt ttcgtcacca tactggccat gcgcggaata gctgtaggtg aagccgctgg
    19021 gcagatccca gggacgatag gtgtgaaggt tgaggtcgcc actgatgccg gaatccagca
    19081 tgctggcggt cggcgacttg atgacgtcca cgccgcggaa cagcgtggct ggcagcattt
    19141 cgaagtcggg ctgctgtgaa tcgatctggt tgggcgtgat gaacacctcg ccgttcaaca
    19201 tcgtgcccac ttccggaagg ccgcggacgt ccaccgagct gcccaccccg gcatcgcggt
    19261 tgatctggac gccggtgacg cgctgcaagg catcggtaat ggtgacgtcg ggcaactggc
    19321 cgatgctttc cgccgtgatg ctgtcctgga tgttgggggc gatccgcttg agctcgatcg
    19381 cgcgcatctg ggacgcccgc acgccgacca cgttcaccac cgacagggtc gccggatttg
    19441 ccgcctccgg ggccttggag gtctttttat tgctgtcgtc cgtgggctgc aattgaccgg
    19501 cgacggtagc ggactgcgcg gtggcctgcg tcgacggatc ggtggcctgg ctgcccgggg
    19561 cctgcgacgc cgccgccgag gttgccatca tggcaatcag cgacgcggaa atggcaagcg
    19621 acagcggagt cctgcgatgg atggttggca tggtcttatc cctcgatgat tatcggtaaa
    19681 gcgtcacaag cctgcgtcgt ggcgttgatc ggatcgcggc gcgccggggc tagcgctgta
    19741 ttgcccacac ccgggcatcg tcggtcgtgg cgcggcgggt aatgcccgcc cggcttcacc
    19801 ccgtggctcg cacatcggat cagcgtcatc acgttcacct cgacaacgac acattcgcga
    19861 tcgcccattg gccgtcccgt ggatcgccgg tggcgacgat gcacagcgtt cgcgcaccgg
    19921 cccggctccc ggagtcaact tcggccccga gggtggtctg gtacttcgtg gctgccgcag
    19981 gggccagtgg cagcgtggcc aacaatgtcc cctggcacga atcgccatgg acctggagtt
    20041 cgccggctgc actcgccgcc ggacgcacca ccgcgccgtc gctgtcatcc ccgaatcgcc
    20101 acgcgatccg gccgacggtc acgcggatct gccggattcc atggagcggc gcgtccttcc
    20161 acaaccagca catgtttccc acgtccaccg cgtataccgg cgccgcgccg tgcgcgggtc
    20221 gatagcctgc cagccgcatg ggtggtcgac cggaacatgt ggcgagttgg ctgccgtcgc
    20281 ggatcaggag tgccgtggaa ccgatgacat gggagcgcgg cgccgacagt tcgaagccat
    20341 cgggcgcgaa agtggcggcg cgcacggtcg ttcccgcgcc cagggaaaac tcgagaggcg
    20401 ccgcgtagcg ccgggacgcg gcagtcggtg gcgaaccatc ggtcgtgtag tggattgcgc
    20461 cgaagccggc ctggttcgag agcgtgacgc gcaacttccc gtcctgctcc ggggccagcg
    20521 aaaacgcggg tgcgaacgcg ctgtcggcat aggcgatgcc gagcgcgcga taccgcgcca
    20581 tctcggcagg cattcgcgag aggaaaccat cccattcgtg ggcgtccgcc ggcgaccagc
    20641 ccagttccga caacgcagcg acgcgcggga acaaggcatg ctcggcgcgt gggaagttcg
    20701 gcaccaactc cgtccacagt tgtgcctgca cgcccagcac gtgcttcgct tccgcggacg
    20761 tggctccctc cggcgccagg gcggtgtcgt aggcgtcctt caacgagatc atcccgggtt
    20821 gccccggccc ctcgtcgggc agatccgatt ggtagtagtc gaagtacagc gactccaccg
    20881 gtgcgagcag cacgtcgtgg ccctgctgga ttgcctgcag cgcgacccgc aatttgtcgc
    20941 gcccgtgcca tgtcatcacg acctcggata cgggcaacgt gccgccggcc agaagttcgt
    21001 cgtcccaacc caccggcgtc cggccgtgct tcacgaggta atccgcgagc cgtttggtga
    21061 accagccttg caacgcgtcc atgttcgcga ggccgaggct tcgcatccgg gcgcgcacct
    21121 ccggcgatgc gttccactgg gtcttgtccg cttcatcccc gccgagcgag atgtatctcg
    21181 aagggaacag ccgcatcacc tcgtcgagga catcatcgag gaaccgcaac gtcttctcgt
    21241 ccggcttcag cagccatgcg ctgatgcccc agtcggtcca gacttccggc cgcttgccgg
    21301 tgacgcccag ccacggatag gcggcgacgg cagcctggga atgcccggga acgtcgatgc
    21361 ccgggacgat ggtgacgaag cgcgcggctg catggcgcac gatttcgcgc acctgggcct
    21421 cggtgtagaa cccgcagtac ggcttgtccg gcgaacctgt cagttcgaca tcgagaccca
    21481 cggccttgcg gcacgcgccg atgctggtga gctccggata cttcggcact tcgaggcgcc
    21541 agccctggtc ttcggtcagg tgccacagca acacgttgag cttgttgagc gacatccagt
    21601 cgatcagctt ctcgatctcc gcgacgctct ggaagtggcg gccggagtcg agcaacaggc
    21661 cacgccaggc aaaacgtggg tggtcatcga tggatccctc cgcgacgtat gccgtggaat
    21721 gcggcttcca gccgggcggc gtcaacaatt gccaggcggt gacggcgccg tagtagagcc
    21781 cgcggggctt ccgcgcgacc acgcggatgg cgccatcgcc gattgcgatg tggtaaccgg
    21841 cgtcaccggt gacgtcggcc tgcggatcga ggtcgaaaac gatggctgaa tgcgtggccg
    21901 gcgacccatc caggcgcaag tgcaggccac gcgtactccc gacccatccc acgaactgct
    21961 ttgcaatcgg gagagcctgc gtatgctgcg agcccgacag cgcgaccgtg gcgccgtcgg
    22021 cgaccgcgac cacgccgttc ccggccggat gcattgcggc gggttgcggc agcagtgacc
    22081 acgccgacgc cgcagtcttg gcgcccgcca aaggcaccca gagcgtggcg aacaccacga
    22141 atgcgagacc ggcgtggatg ccgttcgatc gcggacggcg ccagcggcga cctggccgcg
    22201 gatcgatggc aacgtttgcc gtcatcccgc caccccgcac gccgccaccc gctcatcttc
    22261 cgggcgatcg tgcgacagct ggtcgaggta ccacctggcg gcaccgagca acccatgctg
    22321 gccatgctcg atcacgcgta ccggcacgcg cgacaggaac tcgcgaacgc tgcgttcgtg
    22381 caggaaccgt tcggcgaagc ggctttgccg cagcagatcg aaaatcgacg acaggaaccc
    22441 cccggcgaga tacacgccgc cacgcgccat gaataccatc gcgaggttgc cgacgaagct
    22501 tcccaacacg tcgcagaaca tcccgacggc ttccacggct tgccggtcga cgcgtgcccg
    22561 cgcggcggcg gtaatggcct ccggcgtcgc cagtgccggc gggtccccgt tcaacccgca
    22621 cagcgtccgg tacaaggtca gcaacccggg gccggacacg atggactcgt acggcacgta
    22681 tccgccgccg ggcgacacgc tccgtaacac ctcgcgctcg cgaatggttt gcggcgcgaa
    22741 gtccatctgc cccgcttccg tcgtcaacac gacacttcgt gcacggccgg caagtcgtac
    22801 ggccgcgccg agtccggtcc cgggcccgac cacaagcgtc ggtcccttcg cacacgtgtc
    22861 gggaccgcac aacaactcgc cgccagcgct gcggatctca tccagcgcgt agccgagcgc
    22921 ctcgaagtcg ttcagcacgg cgacgtcgtc gaactccagg gccttgcgca gccgcgagag
    22981 cgtgatcggc catgggagat tgtcgtgaat gacctt
//
LOCUS       DASSNH010000189         9060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_158029, whole genome shotgun sequence.
ACCESSION   DASSNH010000189 DASSNH010000000
VERSION     DASSNH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158029"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFK00_07075"
     CDS             complement(<1..312)
                     /locus_tag="VFK00_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592748.1"
                     /translation="MPRYTFTDLGTLGGTFSLAYGINNGGQIAGFSTLVGDAAEHSSV
                     WEFGRTTDLGTLGGPDSQSFTGLNEFGAVPGVTELASPDPNGEDFCGIGTHLTCLGFL
                     WR"
     gene            675..3074
                     /locus_tag="VFK00_07080"
     CDS             675..3074
                     /locus_tag="VFK00_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7592749.1"
                     /translation="MNTAPKIVYAFGPYRADPDQELLLRDGQRVPVTPKAFEVLLALL
                     RRSREVVCKDELMRIVWPDTVVEEANLSQSIFMLRKALGDTSGTRHYIVTLPGRGYRF
                     AEPVRVITSEDANDEDGGDSHQQAQAASPASEGAIAWPPRNRWKYPLLLGAGSIALAV
                     AAAMLVRIHGPAALPTADPVLVADFANRTGDPLFDDTLRQGLAVQLQQSPLLSLISQD
                     RIQQTLRLMDRPGDAALTPALAREICERTSSDIVLDGSIAKLGSAYVVGLQARDCDSG
                     EVLDTEQAQAAKREDVLRALDEIAGRFRSRIGESGAMIARHNTPLAQATTSSLAALKA
                     YSQAVRVQASAGSAAALPLFQRAISIDPRFAMAHALLGRVYGDLGDSARAMQSTSEAW
                     RLRSHSSDTERFFIEASYHLEVTGNMEAAREVCESWIRTYPRAMVPHAFLGGIVEPVL
                     GNYRAAVEHARAAIDLDPDFVIGYGILAYGHQYQGDLEGANTSLDSAAARKLSMPELV
                     VQRYDIAFLRGDEQEMDHQASIAKDLSGAEDWMAFHQSLALAYRGRVRQADVLSEQAT
                     VEALQASQSERAALFQAGAAWRASLFGDVAAAQRHAKAALKLSNNRYVEFGAAFALAL
                     AGHSAQARTFADDLRARFPEDTSLRFSQLPALLGQLAIDDGKPMQAIEALRIAAPYEF
                     GVPRSSMHGSFGALAPVYVRGEAYLALHRGADAAAEFRKVLAHPGVIGSDPVGALARL
                     QLARAYAMQGEEGKAMDAYRALEALWQGADSVLPAVRQATAESAKLRGSATAAALHHP
                     M"
     gene            complement(3059..3799)
                     /gene="phbB"
                     /locus_tag="VFK00_07085"
     CDS             complement(3059..3799)
                     /gene="phbB"
                     /locus_tag="VFK00_07085"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HET7592750.1"
                     /translation="MSARVALVTGGTGGIGTAIVCRLAKMGHKVVTNYRNEEKTRAWR
                     DKLASEGFDVLTVKADVSDPQSCEQMVREIEEKAGPVEILVNNAGITRDTTFHKMNYQ
                     QWMEVVNTNLNACFNVTRPIIEGMRARKWGRIIQISSINGQKGQYGQANYAASKAGMH
                     GFTISLAQENAKFGITVNTVSPGYIGTDMVMAVPEDVREKIVAQIPLGRLGEPDEIAY
                     AVAFFLDDKAAWVTGANLAVNGGHYMGW"
     gene            complement(3891..4769)
                     /gene="gluQRS"
                     /locus_tag="VFK00_07090"
     CDS             complement(3891..4769)
                     /gene="gluQRS"
                     /locus_tag="VFK00_07090"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET7592751.1"
                     /translation="MAYRGRFAPSPTGELHFGSLVAALASWLRARRQGGTWLVRVEDI
                     DPPREVPGSARSILAAFEQLDLVADAPPLYQSQRGDAHAAALARLRDAGHAFACWCSR
                     AELEAAGTVHRDGRCIAPARDDRAPAWRLRVPDLDIAFEDALQGPQRENVRGAVGDFV
                     LKRADGLWSYQLACVVDDAFQGITEVVRGADLLDSTPRQILLQRLLGLPTPAYLHLPL
                     AVDDAGRKLSKSTDAPPVDTRHPRATIAAALQFLGQPVPDAPDATGMLHAAARAFDLS
                     GLHGIAARAANPHLRH"
     gene            4894..6957
                     /locus_tag="VFK00_07095"
     CDS             4894..6957
                     /locus_tag="VFK00_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HET7592752.1"
                     /translation="MNAVSGLPRQRELTLRGVIIGIVITIVFTAANVFFGLKAGLTFS
                     TSIPAAVISMAILKAMRDATIQENNIVQTIASAAGTLSAIIFVLPGLVIVGYWSGFPF
                     WISAGICATGGILGVMYTIPLRRALVTDSDLPYPEGVACAEVLKVGSGASEDIGRMGE
                     AESVPVAADGRGREAPDPAAAVQASSAGLKAVVWGAIVSAIFYVIVQTRVFAAAVTDY
                     FRVGGARGGATGFDFGLSFALFAIGHLVGLWVGVAMLVGAAIGWAWAVPYFTHVHWVA
                     GAAATVAQDGWSHYVRFIGAGAIGVAAIWTLAKLVKPLVAGLAGALRASRARKTGTLH
                     ELPRTEHDMPIGLVGLISLVCMIPIAYLLWHFSVTNGLGDATWTLVIGGVVFVILLSF
                     LVSAVCGYMAGLIGSSNSPLSGVGILVVVIAALLLVVGVKPFLPEGADKALVGFALFI
                     TAIVFAVASIANNNLQDLKTGQLVDATPAMQQWALVIGVLAGAVVIPPVMDLLQHTYG
                     FLGAPGADPSRALPAPQAGLISALAKGVITGGVPWDMIGLGAAIGAGVIIVDEVLGVW
                     KKGPRLPPLAVGLGIYLPTSTTLMVVVGALVGAWFDRHAGRRPNTEGTRQLGVLLASG
                     LIVGESLLAVVFAGIVGLSGKQGPIALVGEGFATPSIILGGVVFAVTVIVLYRWILRM
                     GRATA"
     gene            7196..>9060
                     /gene="htpG"
                     /locus_tag="VFK00_07100"
     CDS             7196..>9060
                     /gene="htpG"
                     /locus_tag="VFK00_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7592753.1"
                     /translation="MTTTTETRPFEAEVAQVLRLVTHSLYSHKEIFLRELVSNASDAC
                     DKLRFEAIARSELLRDDAELHIDVEFDKDAGTITVRDNGIGMSRDEIVANLGSIASSG
                     TRRFLETLSGEQQADARLIGQFGVGFYSAFVVADKVTVLTRRAGAEADEGVRWESDGK
                     GEYSLAAEEIPARGTSVVLHLKDDEKEFLDNWKLRDLIRKYSDHVAFPIRMHKQKDGK
                     PTDEWETVNDASALWAKPKSEITDDDYKAFYKSLGHDFNDPLAWTHNRVEGRQSFTTL
                     LYVPAAPPFDLMMGARDERKGVKLYIKRVFVMDAAEELLPNYLRFVRGVVDADDLPLN
                     VSREILQHNRQIDHIKAACVKRVLDLVEKLASNEPEKFETFRRAFGNTLKEGIVEDPS
                     NRERIAKLLRFASTKGEGGEQKVSLDDYLARMKPGQEAIWYVTADSYKAAAGSPQLEA
                     FRAKDIEVLLMFDRVDEWIMGQFGEYQGKPFRNVAKGELPLDEADKKKQEEVAKEAEP
                     LVAKFKELLGDRVSEVKVSARLTDSPSCLALGDYDLAPHLAPLLREAGQEVPDSKPTL
                     EINPAHPLVRRVQSEADEAQARDLALLLLEQAEITAGAQLPDPAAFVQRMNRMLGG"
BASE COUNT         1513 a         2898 c         3115 g         1534 t
ORIGIN      
        1 gcgccagagg aagccgaggc acgtcaggtg cgtgccgatc ccgcaaaagt cctcgccgtt
       61 cggatccggc gacgcgagtt ccgtgacgcc cggcactgcc ccgaattcat tgaggccggt
      121 gaaggactgg ctgtcggggc cgccgagggt gcccagatcg gtcgtgcggc cgaactccca
      181 caccgaggag tgttccgccg catcgcccac gagcgtcgag aagccggcga tttgcccccc
      241 gttgttgatg ccataggcta ggctgaaggt gccccccagg gtacccaggt cggtgaaggt
      301 gtagcgcggc atcgaatgag ccgagtggcc tcccgccatc gcgccgagcg gcgcaaccgc
      361 gagtgccagt gccaacgctc gtgagcgtgc ccttgccagg tggcttcgcc gggatatcga
      421 atgcttcggt tgcatgattt gttctccttg atggctgcgc gcgggccccg gcagtcgcgc
      481 gacgagcgcg tgccgggccc ggaagtaact tgcgctggct ctgcttcaaa agtcctcatc
      541 gatcccctca aaaatgctca aacctcctga aaccggctat ccacgctcgc aacgtcgctc
      601 ggccgccgcg gtgctagatt ggagccggct cgcagcgacc gatccggtgg cgagcggcgg
      661 gcaggtgggg gcccatgaac accgcaccca aaatcgttta cgccttcgga ccgtaccggg
      721 cggacccgga ccaggaattg ctgctgcgtg acgggcagcg ggtaccggtt accccgaagg
      781 cgttcgaggt gttgctggcc ctcctgcggc gcagccgcga ggtcgtctgc aaggacgaac
      841 tgatgcggat cgtgtggccg gacaccgtcg tcgaggaagc gaacctcagc cagagcatct
      901 tcatgctgcg aaaggcgctg ggggacacct ccggaacgcg ccactacatc gtgacgctgc
      961 cgggccgcgg ctaccgtttt gcggagccgg tgcgcgtcat cacgtcggag gacgcgaatg
     1021 acgaagatgg cggagattcg caccagcaag cccaggcggc ttcccctgca tcggagggcg
     1081 cgatcgcttg gccgccccgg aaccgatgga aatatccgct gctgctcggc gccggatcga
     1141 tcgcgcttgc ggttgcagcc gcgatgctgg tgcgcatcca cggacccgct gcgctgccca
     1201 cggccgatcc ggtgctggtc gccgacttcg ccaaccgcac cggcgacccc ttgttcgacg
     1261 acacgctgcg gcaggggctt gcggtgcaac tccagcaatc gccgctgctc agcctgatat
     1321 cgcaggaccg gatccagcaa accctgcgcc tgatggatcg ccccggggat gctgcgctca
     1381 ctccggcgtt ggcgcgcgaa atctgcgagc gtacatccag cgacatcgtg ctcgatggct
     1441 cgatcgcgaa gctgggcagt gcctacgtgg tcgggctgca agcccgcgat tgcgacagcg
     1501 gcgaagtact cgatacggag caggcgcaag ccgcgaaacg ggaggacgtc ctgcgggcgc
     1561 tggacgaaat cgccggcagg ttcaggagca ggatcggcga atcgggcgcg atgatcgcgc
     1621 ggcacaacac gccgctcgcg caggcgacga cctcatcgct cgctgcattg aaagcgtaca
     1681 gccaggcggt gcgcgtgcag gcgtcggcgg gctccgcggc ggccctgccg ctgttccagc
     1741 gcgcgatctc catcgacccc cgtttcgcga tggcacatgc gttgctgggg cgagtctacg
     1801 gcgacctcgg agattccgcg cgcgccatgc aaagcaccag cgaagcttgg cggttgcgca
     1861 gccacagcag cgatacggaa aggttcttca tcgaagcgtc ctaccacctc gaggtgaccg
     1921 gcaacatgga ggcggcgcga gaggtgtgtg aatcctggat acgcacctac ccgcgcgcga
     1981 tggtgccgca cgcctttctg ggcggcattg tcgaaccggt gctgggcaat tatcgcgcgg
     2041 cggtcgaaca cgcgcgcgcc gcgatcgacc tggatccgga tttcgtgatc ggttacggca
     2101 tcctcgccta cgggcatcaa taccagggtg atcttgaagg tgcgaacacg tcgctggaca
     2161 gcgccgcggc gcgcaaacta tccatgcccg aactggtcgt gcagcgctac gacatcgcat
     2221 tcctgcgcgg tgacgaacag gaaatggatc accaggcgag catcgcgaaa gacctgtcgg
     2281 gcgcggagga ctggatggcg ttccatcaga gccttgcatt ggcgtaccgg gggcgggtgc
     2341 ggcaggcgga cgtgctgtcc gagcaggcga cggtggaggc attgcaagcc tcgcaaagcg
     2401 agcgcgcagc actcttccag gccggcgccg cctggcgcgc gtcgctgttc ggggatgtgg
     2461 ccgccgcgca acgccacgcg aaggctgcgc tcaaactgtc gaacaaccgc tatgtggaat
     2521 tcggcgccgc ctttgcattg gcgctggcag ggcattccgc gcaggcgcga acgttcgcgg
     2581 atgacctgcg agcgcgtttt cccgaagaca cctcgctacg cttcagccag ctgccggcgt
     2641 tgcttgggca actcgcgatc gacgacggca aaccgatgca agccatcgaa gcgttgcgaa
     2701 tcgccgcacc ttatgagttc ggggttcctc gcagcagcat gcacggatcg ttcggcgcgc
     2761 tcgctcccgt gtatgtgcgc ggcgaggcct atctcgcgct gcaccggggg gccgacgctg
     2821 ccgcggaatt tcgcaaggta ctcgcgcatc ccggggtgat cggaagcgac ccggtcggcg
     2881 cgctggcacg cttgcaattg gctcgcgcct acgcaatgca gggcgaagag ggcaaggcca
     2941 tggatgcgta ccgcgcgctg gaggcgttgt ggcaaggcgc ggactccgtc ctgcccgcgg
     3001 tgcgccaggc caccgcagaa agcgcgaaac tgcgtggaag cgcgactgca gccgcgcttc
     3061 accaccccat gtaatgcccg ccattgacgg cgaggttcgc gccggtgacc caggcggcct
     3121 tgtcgtcgag gaagaacgcg acggcgtagg cgatttcgtc gggttcgccg aggcgcccca
     3181 gcggaatctg cgcgacgatc ttctcgcgca cgtcctcggg cacggccatc accatgtcgg
     3241 tgccgatgta gcccggcgac acggtattga cggtgatgcc gaatttcgcg ttttcctgtg
     3301 ccagcgaaat cgtgaaaccg tgcatgccgg ccttcgacgc cgcgtagttc gcctggccgt
     3361 actggccctt ctggccgttg atggaactga tctggatgat ccgcccccat ttgcgcgcgc
     3421 gcatgccttc gatgatcggg cgggtgacgt tgaagcacgc gttcaagttc gtgttcacca
     3481 cttccatcca ctgctggtaa ttcatcttgt ggaaggtggt gtcacgggtg atgccggcgt
     3541 tgttgacgag gatctcgacc ggcccggctt tttcttcgat ctcgcggacc atctgctcgc
     3601 aggactgcgg atcggacacg tcggccttga cggtgaggac atcgaagccc tcgctcgcca
     3661 gcttgtcccg ccacgcgcgc gttttctcct cgttgcggta gttcgtgacg accttgtgcc
     3721 ccatcttcgc caggcggcag acgatcgcgg taccgatgcc cccggtaccc cccgtaacca
     3781 gtgccacgcg tgcgctcatg acgactcccc atcggtggtg gtgactccat cctacccgct
     3841 tcccgagcat tcgggcgcgg ccgcgaaacg gttgcagagg cgttcgaaga tcagtgccgc
     3901 aggtgcggat tggcggcacg cgcggcgatg ccgtgcaggc cggacagatc gaaggcccgg
     3961 gccgcggcgt gcagcatgcc ggtcgcgtcc ggcgcgtcgg gaaccggttg cccgaggaat
     4021 tgcagggcgg ctgcgatcgt cgcccgcgga tggcgggtgt ccaccggcgg tgcatcggtg
     4081 gatttggaga gcttgcgtcc cgcgtcatcc accgccagcg gcaggtgcag gtacgcgggc
     4141 gtcggcagcc cgagcaggcg ttgcaggagg atctggcgcg gcgtcgaatc cagcaggtcg
     4201 gcgccccgca cgacttccgt gatgccctgg aaggcatcgt ccacgacgca cgcgagctgg
     4261 tagctccaca agccgtcggc gcgcttcagc acgaaatcgc cgaccgctcc gcgtacgttt
     4321 tcgcgctggg ggccctgcag cgcatcctcg aatgcgatat cgagatcggg gacccgcagc
     4381 cgccacgccg gagcgcggtc gtcgcgcgcc ggggcaatgc agcgtccgtc gcggtgcacg
     4441 gttccggcgg cttcgagttc ggctcggctg caccagcaag cgaaagcgtg acccgcatcg
     4501 cgcagccgcg ccagcgctgc cgcatgggca tcgccccgct gcgactgata cagcggcggg
     4561 gcatcggcca cgagatccag ttgctcgaag gcggcgagga tcgatcgtgc ggaacccggc
     4621 acttcgcgcg gcggatcgat gtcctcgacc cgcacgagcc aggtgccgcc ttgccggcgc
     4681 gcacgcagcc agctcgccag cgccgcgacc agcgacccga aatgcagctc gccggtcggt
     4741 gagggtgcga agcggccgcg gtaggccaag gaacctcctt tgcgcgatgc tgtgcggcgg
     4801 ctagtatgcg ggcgggatgg cccgcgaagg accggccatc ccgccgcggg ccaccatggc
     4861 ggccgcggaa ccaccgatcc aggggagcca cgcatgaacg ccgtttcagg attgcccagg
     4921 cagcgcgaac tgacccttcg tggcgtgatc atcggcatcg tgatcacgat cgtgttcacc
     4981 gccgcgaacg tgtttttcgg gttgaaggcg ggcctgacct tttcgacatc catcccggcc
     5041 gcggtgatct cgatggcgat cctgaaagcc atgcgcgacg ccacgatcca ggaaaacaac
     5101 atcgtgcaga cgatcgcttc ggcggccgga acgctgtcgg cgatcatctt cgtgctgccg
     5161 gggctggtga tcgtcggata ctggagcggt ttcccgttct ggatttcggc cggcatctgc
     5221 gcaaccggcg gcatcctcgg ggtgatgtac acgatcccat tgcggcgggc gctggtcacc
     5281 gattccgatc tgccttaccc ggaaggcgtg gcgtgcgccg aggtgctgaa ggtcggcagc
     5341 ggggcgtccg aggacatcgg ccggatgggc gaggcagagt ccgtgcctgt cgctgccgac
     5401 ggccgcgggc gggaggcgcc cgatccggcc gcggcagtgc aggccagcag cgcgggcctg
     5461 aaggccgtgg tgtggggcgc gatcgtctcg gcgattttct acgtcatcgt gcagacccgg
     5521 gtgttcgccg ccgcggtgac cgactatttc cgggtcggcg gtgcgagggg aggcgcgacc
     5581 ggcttcgact tcgggttgtc gttcgccttg ttcgcgatcg ggcatctggt cggactgtgg
     5641 gtcggcgtgg cgatgctggt gggcgcggcg atcggttggg cgtgggcagt accgtacttc
     5701 acgcacgtgc actgggtcgc gggcgcggcg gccacggttg cgcaggacgg ctggagccac
     5761 tacgtgcgtt tcatcggcgc gggcgccatc ggcgtggcgg cgatctggac gcttgccaaa
     5821 ctcgtgaagc cactggtggc gggactcgcg ggtgcgttaa gggcgtcacg cgcgcgcaag
     5881 accggcacgc tgcacgaact gccgcgcacc gagcacgaca tgccgatcgg cctggtcggg
     5941 ctgatctcgc tggtctgcat gatcccgatc gcgtacctgc tatggcactt cagcgtcacc
     6001 aacggcctgg gcgacgcgac gtggacgctc gtgatcggcg gcgtggtgtt cgtgatcctg
     6061 ctgagcttcc tggtgtcggc cgtgtgcggc tacatggccg gcttgattgg ttcatcgaac
     6121 agcccgctgt cgggcgtcgg aatcctggtg gtggtgatcg ccgcactgct gctggtggtg
     6181 ggggtgaagc cgttcctgcc ggagggcgcg gacaaggcgc tggtgggatt cgcactgttc
     6241 atcaccgcga tcgtgttcgc ggtcgcgtcg attgccaaca acaacctgca ggacctgaaa
     6301 accgggcaat tggtggatgc cacgcccgcc atgcagcagt gggcgctggt gatcggcgtg
     6361 ctggccggcg cggtggtgat cccgccggtg atggacttgt tgcagcacac ctacggtttc
     6421 ctcggcgcac caggcgccga tccctcgcgc gcgctgccgg ccccgcaggc cggcctgatc
     6481 tcggcactgg cgaagggcgt gatcaccgga ggggtgccgt gggacatgat cggcctgggt
     6541 gccgcgatcg gtgccggagt catcatcgtc gacgaggtgc tgggcgtctg gaaaaaaggc
     6601 ccgcggttgc cgcccctcgc ggtggggctc ggcatttacc tgcccacttc gaccaccttg
     6661 atggtcgtcg tcggcgcgct ggtcggcgcg tggttcgacc ggcacgctgg gcgacgcccg
     6721 aacaccgaag ggaccaggca actgggcgtg ctgctggcct ccggcctgat cgtgggtgaa
     6781 agcctgctgg cggtggtttt cgcgggcatc gtggggctga gcggcaagca gggaccgatc
     6841 gcgctggtcg gggaaggctt cgcgacgccg tcgatcatcc tcggcggggt ggtcttcgcc
     6901 gtcaccgtga tcgtgctcta tcgctggatt ctccggatgg gccgcgcaac ggcttgagaa
     6961 accatcgaac cgctaccaac taggcgactt ggctgcccgt cgcttgtcgc tggcctccgg
     7021 ctgttcattg tgggcctgcg cagcgcgcag aagccgggat ctgctgtgcc acaaccttcg
     7081 atgaaaggca ggttccgggt tccgcccgca cacggcgcga gcggcccgaa tggcgaagtt
     7141 gtggaaatct cgcgcaaacg aacccgtatg ccctgactgt catggaaatg cccttatgac
     7201 caccaccacc gaaacccgtc cgttcgaagc cgaggtcgcc caggtcctgc gcctggtgac
     7261 ccactcgctg tattcgcaca aggaaatctt cctgcgcgaa ctggtctcca atgcttccga
     7321 cgcctgcgac aagctgcgct tcgaagcaat cgcccggtcc gagctcctgc gcgacgacgc
     7381 cgagctgcac atcgacgtcg agttcgacaa ggatgccggc accatcaccg tgcgcgacaa
     7441 cggcataggc atgagccgcg acgagatcgt cgcgaacctg ggctcgatcg cgagttccgg
     7501 gacacgtcgg ttcctcgaga ccctgtcggg cgagcagcag gcggacgcac gccttatcgg
     7561 ccagttcgga gtgggcttct attccgcttt cgtggtggcc gacaaggtga cggtgctgac
     7621 ccgtcgtgcg ggcgcagaag cggacgaagg cgtgcgctgg gagagcgacg gcaagggcga
     7681 gtattcgctc gcggcggagg agattcccgc acgcggcaca tcggttgttc tgcatctcaa
     7741 ggacgacgaa aaggagttcc tcgacaactg gaagctgcgg gacctgatcc gcaagtactc
     7801 cgaccacgtc gccttcccga tccggatgca caagcagaag gacggcaagc cgaccgacga
     7861 gtgggaaacc gtcaacgatg cctcggcgct gtgggccaag ccgaaatcgg aaatcacgga
     7921 cgacgactac aaggcgttct acaaatcact gggacacgat ttcaacgatc ccctggcgtg
     7981 gacccacaac cgggtcgaag gccgccagag tttcaccacc ctcttgtatg tgccggcggc
     8041 gccgccgttc gacctgatga tgggcgcccg ggacgagcgc aagggcgtga agctctatat
     8101 caagcgggtc ttcgtcatgg atgcggccga ggagctcctg cccaactatc tgcgattcgt
     8161 gcgcggcgtg gtggacgcgg atgacctccc gctcaacgtc agccgcgaga tcctccagca
     8221 caatcgccag atcgaccaca tcaaggccgc ttgcgtgaaa cgcgtgctgg acctggtcga
     8281 gaagctggcg tcgaacgagc cggaaaagtt cgaaaccttc cgcagggctt tcggcaacac
     8341 gctgaaggaa ggcatcgtcg aggatccgtc gaaccgcgag cgcatcgcca agctgctgcg
     8401 cttcgcgtcg accaagggcg agggcggcga gcagaaggtt tccctggacg actacctcgc
     8461 gcggatgaaa cctgggcagg aggccatctg gtacgtcacc gccgacagct acaaggcggc
     8521 ggcgggcagc ccgcagctcg aggccttcag ggcgaaggac atcgaggtgc tgctgatgtt
     8581 cgatcgcgtc gacgaatgga tcatgggtca gttcggcgag taccagggca agccgttccg
     8641 gaacgtcgcc aagggcgagt tgccgctgga cgaagccgac aagaagaagc aggaagaggt
     8701 cgcgaaggaa gccgagccgc tggtcgcgaa gttcaaggaa ctgctgggcg atcgcgtctc
     8761 cgaagtgaag gtttccgcgc gcctgaccga ttcgccctcg tgcctcgcgc tcggcgacta
     8821 cgacctcgcg ccgcatctcg cgccgctgct ccgcgaggcg ggccaggaag ttccggactc
     8881 caagccgacg ctggaaatca atcccgcgca tccgctggtc cggcgcgtgc agtcggaggc
     8941 cgacgaggcc caggccaggg atcttgcgct gctgctgctc gagcaggccg agatcaccgc
     9001 cggcgcccaa ttgcccgatc ccgcggcatt cgtgcagcgg atgaaccgga tgctcggcgg
//
LOCUS       DASSNH010000190         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_5717, whole genome shotgun sequence.
ACCESSION   DASSNH010000190 DASSNH010000000
VERSION     DASSNH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5717"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFK00_07105"
     CDS             <1..1020
                     /locus_tag="VFK00_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET7592754.1"
                     /translation="TGVCHTDAYTLSGADPEGLFPVILGHEGGAIVEEVGPGVTTVKP
                     GDHVIPLYTPECGECEYCTSGLTNLCQKIRVTQGEGVMPDGTSRFSHKGRMLRHYMGT
                     STFSEYTVLPEISVAKIDPKAPLDKVCLLGCGITTGVGAVLNVAKVRPGSTVAVFGLG
                     AIGLAVVQGAVMAKAERIIGIDINPGKWTMAEAMGATDFVNPGDHAGPIQQVIVEMTR
                     GGVDYSFECIGNVNVMRAALECCHKGWGESTIIGVAGAGQEISTRPFQLVTGRVWRGA
                     AFGGVKGRSQLPGYVDSYMRGKLKVDEFVSKVLPLEKINEAFDLMHEGKVIRPVIRHE
                     SPAEA"
     gene            1400..>3181
                     /locus_tag="VFK00_07110"
     CDS             1400..>3181
                     /locus_tag="VFK00_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592755.1"
                     /translation="MIGTLGAAALLLPPLAGAQSTGSTQQGAPQSLPTVVVTGTRIPE
                     PEFDVPASISVVTSKEIRSGPPGTNLSQTLARVPGLVAQNRQSLAQDLQLSLRGFGAR
                     ASFGVTGIRLLVDGLPYSDPDGQGQTDPFDLAAAERVEVLRGPFSVLYGNAAGGVIQV
                     FTKDGPAQPEVGVGTMVGSYGTVENQLNAGGTAGRLNYMANVSWFQTDGYRQHSAAER
                     DHLYSKLRYEPNDDASLTFIFNAENQPFAEDPSALSAEQVAEDPRQAVSRVFQFGSGE
                     FHRHRQGGVVYRQKLGERDELQATAWLGSRRVVQFLPFSGDDPLSGGAVIDLDNDAGG
                     GDARWTHRATLAGFPLTTTAGFDYQRLHERRKGFVNDDGRKGPLARDEDDILSRLGAY
                     AQAQWVLGRWQLVGGVRQSRVSFEVDDHFITDVDPDDSGRQAFSQATGFASVLYELAP
                     AVNGYASFGRGFETPSFAELAYRPDGTSGLNFDLQPSTSNNYEAGLKADFGLRGKLRL
                     AFYKIDTANEIVTAVSANGRSTFRNAGSTTRKGVELSVQGGLGGGFQGYLAWTWLQAD
                     YAAGPSAGNRLPGVPRTTLYGELDWTYA"
BASE COUNT          539 a         1030 c         1093 g          519 t
ORIGIN      
        1 accggtgtgt gccacaccga tgcctacacc ttgtcgggtg ccgacccgga gggcctgttc
       61 ccggtgatcc tgggccacga gggcggcgca atcgtcgagg aggtcgggcc gggcgtgacc
      121 acggtgaagc cgggcgacca cgtgattccg ctgtacacgc ccgaatgcgg cgagtgcgag
      181 tattgcacgt cgggcctgac caacctgtgc cagaagatcc gcgtgacgca gggcgagggc
      241 gtgatgccgg acggcacctc gcgcttttcc cacaagggca ggatgctgcg gcactacatg
      301 gggaccagca cgttttccga gtacacggtg ctgccggaaa tctcggtcgc gaagatcgat
      361 ccgaaggcgc cgctcgacaa ggtgtgcctg ctgggttgcg gcatcaccac cggcgtgggt
      421 gcggtgctga acgtcgcaaa ggtgcggccc ggttccaccg tcgccgtgtt cggcttgggt
      481 gcgatcggct tggcggtggt gcagggcgcg gtgatggcga aagccgaacg catcatcggc
      541 atcgacatca atcccggcaa gtggacgatg gccgaagcga tgggcgcgac cgacttcgtg
      601 aatccaggcg accacgccgg cccgatccag caggtgatcg tcgagatgac ccgcggcggc
      661 gtcgactatt cgttcgagtg catcggcaac gtcaacgtga tgcgcgccgc gctcgagtgc
      721 tgccacaagg gctggggcga atcgaccatc atcggcgtcg ccggggcggg gcaggagatc
      781 agcacgcgtc cgttccagtt ggtcaccggc cgggtctggc gcggggcggc cttcgggggc
      841 gtcaagggcc gttcgcaatt gccgggctac gtcgattcgt acatgcgggg caagctcaag
      901 gtggacgaat tcgtgtcgaa agtgctgccg ctggagaaga tcaacgaggc cttcgacctg
      961 atgcacgaag gcaaggtcat tcgtccggtc atccggcacg aaagtcccgc ggaggcgtaa
     1021 ggcgaatcca tccggcagga gaatccgcac gggcggagga tggtccgctc ctgcaccgtg
     1081 ccccggtttt cgaatccatc ctgccgcccg cgcggacgcc gccatgtctt cgccaagccg
     1141 cgtgatgccg gcttcacgag gtctgcgtta cggtcggcag tgacaccggt accgcctcgc
     1201 tggctccttg gcctgcgcca tgacttcgca ggagctgggc atcgagactg actcccgttg
     1261 caatccaccg cacggggtgc ccgtacccac aaggaagctt gcgatggcag gtcctggaaa
     1321 cttgtacccg acgcgccggc gacgtgccgc agggttggca cgcgggatgc cgccgtatcg
     1381 acgacgattg ccgctcgccg tgatcgggac gctcggagcg gcggcactgc tgttgccgcc
     1441 gctggccggt gcgcagtcga ccggctcgac gcaacagggc gcgccgcagt cactgccgac
     1501 cgtggtggtc accgggacac ggattcccga accggaattc gacgtgccgg catcgatcag
     1561 tgtggtgacg agcaaggaaa tacgttcggg tccgccagga acgaatctct cgcaaacctt
     1621 ggcgcgggtc ccgggcttgg tcgcgcaaaa ccgccaaagc ctcgcccagg acctgcaact
     1681 ttcgctccgc ggcttcggcg cgcgcgcatc cttcggcgtg acgggaatcc ggttgctggt
     1741 ggacggcctt ccttattcgg atcccgacgg acagggccag accgatccgt tcgacctcgc
     1801 cgcggccgag cgcgtggaag tgctgcgcgg gccgttctcg gtgctctacg gcaacgccgc
     1861 cggtggcgtc atccaggtct tcaccaaaga tgggccggcg cagcccgagg tcggcgtggg
     1921 cacgatggtc ggcagctacg gcaccgtcga gaaccagctc aacgccggcg gaaccgcggg
     1981 acgtctcaat tacatggcca acgtgtcatg gttccagacc gacggctatc gccagcacag
     2041 cgcggcggaa cgcgatcacc tctacagcaa attgcgctac gagcccaatg acgatgcatc
     2101 gctgactttc atcttcaacg ccgaaaacca gccgttcgcg gaggacccct cggcgctgag
     2161 cgccgaacag gttgccgagg acccgcggca agccgtctcg cgggtgttcc agttcggctc
     2221 cggcgagttc caccgccacc gccagggcgg cgtggtgtac cggcagaaac tcggcgaacg
     2281 cgatgagctg caggcgaccg catggctggg cagccggcgc gtggtgcagt tcctgccgtt
     2341 cagcggcgat gatcccttgt cgggcggcgc ggtcatcgac ctcgacaacg acgccggcgg
     2401 cggcgacgcg cgttggacgc atcgcgccac gcttgcaggt tttccgctga ccacgacggc
     2461 ggggttcgac taccagcgcc tgcacgaacg gcgcaagggc ttcgtcaatg acgacggtcg
     2521 caagggcccg cttgcccgcg acgaggacga catcctttcg cggctcggtg cgtacgcgca
     2581 ggcgcagtgg gtgctcggac gttggcaatt ggtcggcggc gtgcgccagt cgcgcgtcag
     2641 cttcgaggtc gacgaccact tcatcaccga cgtggatccg gatgacagcg gcaggcaggc
     2701 gttttcgcag gcaaccggat tcgccagcgt gctttacgag ctggccccgg cggtgaatgg
     2761 ctacgcaagt ttcggccggg gcttcgaaac gccgtcgttc gccgaactcg cgtatcggcc
     2821 ggatgggacc tccggcctca acttcgacct gcagccgagt accagcaaca actacgaggc
     2881 gggcctcaag gcggatttcg gcctccgggg caagctgcgc ctggcctttt acaagatcga
     2941 cacggccaac gagatcgtca cggcggtgtc cgccaacggt cgctccacct tccgcaacgc
     3001 cggcagcacg acacgcaagg gcgtcgagct gagcgtgcag ggcggcctcg gcggcggctt
     3061 ccagggttac ctcgcgtgga cctggttgca ggcggactat gctgcgggtc cgtccgccgg
     3121 caatcgcttg ccgggcgtgc cgcgcaccac gctgtacggc gaactggact ggacctacgc
     3181 a
//
LOCUS       DASSNH010000191         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_6231, whole genome shotgun sequence.
ACCESSION   DASSNH010000191 DASSNH010000000
VERSION     DASSNH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6231"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..807)
                     /locus_tag="VFK00_07115"
     CDS             complement(121..807)
                     /locus_tag="VFK00_07115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7592756.1"
                     /translation="MGAAADAGIHLAQPGIAPRHAQIERRGARVQLQPCAEPVRLNGR
                     PVHDAIVLKAGDTLEFADVRCRAVSVATEAELDDGSTRMRATVPRYVLRGVSGPTFGR
                     SFGVGGEMVFGRQADCDVCIPIDEISRRHARIRPANGGVLVEDLDSANGTYINGKRIR
                     SGLLHPGEELALDTVRFMLLAPGADMAAPAAAPAEPMDERGGNGLRWAIGVIAALILA
                     AAATYFAGLW"
     gene            981..1412
                     /locus_tag="VFK00_07120"
     CDS             981..1412
                     /locus_tag="VFK00_07120"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HET7592757.1"
                     /translation="MDQALLPLSICTAAGLLWYAAMRARELAIGHASRLCAEHGAQML
                     DQSVALHGLKPLWRGGPPRLARSYRFELSYDGDDRHRASLTLIGDRLVDYSLPARPDP
                     FAPVPPPAPLPRTAPSAPPSAPIASSGGNVVPITRGRRTLH"
     gene            complement(1522..2001)
                     /locus_tag="VFK00_07125"
     CDS             complement(1522..2001)
                     /locus_tag="VFK00_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HET7592758.1"
                     /translation="MAEDQPAMTSNRPYLLRAIYDWISDNGFTPYILVDAGRPGVRVP
                     ASAIREGKVVLNVAMRAVANLDLGNDRVRCMARFGGVSQAIDVPVPAVLAIYAQENGQ
                     GMMFPQEDVEGEPAGDEAPQRPSLQTVAPGNQSDDSPPTPSPDEPPPKGPPKLRVIK"
     gene            complement(2140..>2729)
                     /locus_tag="VFK00_07130"
     CDS             complement(2140..>2729)
                     /locus_tag="VFK00_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7592759.1"
                     /translation="SDNVQCHRVRMVLAAKGVGYDRVDVAVGKPSEELLEFNPYGDVP
                     TLLDRDLVLYQPALICEYLDERYPHPPLMPVDPLSRARLRLACVRIEREWLPLIETIQ
                     GGGRTAEPARKRLRDALMAALPLFKASKFFLNPEMSLADCLVSPVIWRLPMLNVQLPR
                     EGKPIVDYGERIFRGQGFARSLTPDERSLHEVANG"
BASE COUNT          431 a          972 c          868 g          458 t
ORIGIN      
        1 ggattacatt tcgatcggcg cgatcaccaa gaacattcgc gcgatcgatc tttcgatgcg
       61 gtttttgcaa agcgggtttt gacgtgcctt cggtaccgcc ggcgacccga tcaacgccgc
      121 ctaccagagt ccggcgaagt acgtcgctgc ggcagccagg atcagcgcgg cgatgacgcc
      181 gatcgcccag cgcaaaccgt tgcccccgcg ctcgtccatc ggttcggcgg gcgccgccgc
      241 aggcgccgcc atatccgcgc ccggcgccag cagcatgaag cgcacggtgt cgagcgcaag
      301 ctcctcgccc ggatgcagaa ggccgctgcg gatgcgcttg ccgttgatgt aggtgccgtt
      361 ggccgaatcc agatcctcga ccagcacacc gccgttcgcg ggccggatgc gggcatgccg
      421 acgcgagatt tcatcgatcg ggatgcaaac gtcgcaatcg gcctggcggc cgaacaccat
      481 ttcgccgccc actccgaagc tgcgtccgaa ggtcgggccg gaaacgccgc gcaacacata
      541 gcgcggcacc gtcgcgcgca tgcgcgtgct gccgtcgtcg agttcggctt ccgtcgccac
      601 gctgaccgcg cggcagcgca catcggcaaa ttccagggtg tcgccggcct tcaacacgat
      661 ggcatcgtgc accggccgcc cgttcaggcg aaccggctcc gcacacggct gcagctgcac
      721 ccgcgcgcca cgtcgttcga tctgcgcatg gcgcggcgcg atgcccggct gggccaggtg
      781 aatgccggcg tcggccgccg cgcccactac cgtgacgcca tcggtcagcg tcaccgggcc
      841 gtgctcaccg cctgcaaaaa ccagtttcat cttgtgcccc ttggatgcgc gtattcttcc
      901 ctgatgcgtt tctgccgact ggtacacccc tgccggaagt cacgcctgct taaaatcccg
      961 tgcatcttag ccgaaatccc atggaccaag cactgctccc gctctcgatt tgcaccgccg
     1021 ccggcctgct ctggtacgcc gccatgcgcg cccgggagct ggccatcggg cacgccagcc
     1081 ggctgtgcgc cgagcacggc gcgcagatgc tggaccagag cgtggccctg cacggcctga
     1141 agccgctctg gcgcggcggc ccgccgaggc tggcccgcag ctatcggttc gagctgagct
     1201 acgatggcga tgaccgccac cgcgccagcc tcaccctgat cggcgaccgg ctggtggatt
     1261 acagcctgcc ggcacgcccc gatccgtttg cacccgttcc tccgccggcg cccttgccgc
     1321 gaaccgcgcc atccgcgccg ccttccgctc cgatcgcttc ctccggcggc aacgtggtgc
     1381 cgatcacccg cgggcggcga acgctgcact gaagcagcgg ctcaaccata tccacctgct
     1441 ggccggctga ccccctcatc aattccggat ccccgtgtcg tcgcgcatcc ctgtgctgcg
     1501 cgggatgggt tcatcctgaa ctcactttat gacccgcaat ttcggcggcc ccttgggcgg
     1561 cggctcatcc ggcgaaggtg tcggcggcga atcgtcggat tggttgccgg gagcgaccgt
     1621 ctgcaatgac gggcgttgcg gcgcctcgtc gcccgccggc tcgccttcca cgtcctcctg
     1681 cggaaacatc atcccctgcc cgttctcctg ggcgtagatc gccagcacgg ccgggaccgg
     1741 cacgtcgatg gcttggctca cgccgccgaa acgggccatg cagcggaccc ggtcgttgcc
     1801 gaggtccaga ttggcgaccg cgcgcatggc gacgttcaac accaccttgc cctcgcggat
     1861 cgcgctggcc ggcacgcgca cgccgggccg tccggcgtcg accaggatgt acggggtgaa
     1921 accgttgtcg ctgatccagt cgtagatcgc ccgcaacaga tacggacggt tcgaagtcat
     1981 tgcgggttgg tcttcagcca cttcgttttc cggtgcagca agctgcattg ggtcgccggt
     2041 tgccggcatg atgcgtgacg tcattcccgc gcgagccgct gcgcggcgtc gggaatgaac
     2101 gctgttctgg aacccggctc ccgggcccga acccgcagtc taaccgtttg ccacttcgtg
     2161 gagcgaacgc tcgtcgggcg tgaggctgcg cgcgaaaccc tggccgcgga aaatgcgttc
     2221 gccgtaatcc acgatcggct tgccctcgcg cggcaattgc acgttgagca tgggcaggcg
     2281 ccagatcacc ggcgaaacca ggcagtcggc caggctcatt tcgggattga ggaagaactt
     2341 cgaagccttg aacagcggca gcgcggccat cagcgcgtcg cgcaaacgct tgcgcgcggg
     2401 ctccgcggtg cgcccgccgc cctggatggt ttcgatcagc ggcagccatt cgcgctcgat
     2461 gcgcacgcag gcaaggcgca gccgcgcgcg ggaaagcgga tccaccggca tcagcggcgg
     2521 atgcggataa cgttcgtcga ggtattcgca gatcagcgca ggctggtaga gcaccaggtc
     2581 gcggtcgagc agggtcggca cgtcaccgta cgggttgaac tccagcaact cctccgacgg
     2641 cttgccgacg gccacgtcca cgcgatcgta gccgacgccc ttggccgcca gcaccatgcg
     2701 cacgcgatgg cattgcacgt tgtccgacg
//
LOCUS       DASSNH010000192        10169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1392073, whole genome shotgun sequence.
ACCESSION   DASSNH010000192 DASSNH010000000
VERSION     DASSNH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392073"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /gene="mutS"
                     /locus_tag="VFK00_07135"
     CDS             complement(<1..2486)
                     /gene="mutS"
                     /locus_tag="VFK00_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7592760.1"
                     /translation="MDHAELARHTPLMRQYFAAKAEHPHTLLFFRMGDFYELFYDDAR
                     KAARLLDITLTQRGESAGAPIPMAGVPYHAMESYLAKLVRAGESVAICEQVGDPGGKG
                     PMERKVVRVVTPGTLTDEALLPERRDNLLLAIAAGQSGFGLAWVDLAGGRFLLCEVAD
                     ADALAAELARLAPAEILVCEDVTWPPAVTASNGLRMRPPWHFDETSGRRELTRFFGTR
                     DLSGFGADGLSLAVAAAGCLLGYLQETQKAALPHLTGLAIESADETVAMDAATRRNLE
                     LDTHPSGDTRYTLLGVLDSTITPMGARALRRWLHRPLRDHATLRLRHQALAALIESGV
                     LDALREKLRAIGDLERILSRVALRSARPRDLATLRDGLAAAPDLHATLAKLDSPLLHE
                     LLERLGEHQGTVQLLQAAIVERPPALLREGGAIGDGYDAELDELRRLATHADQYLVEL
                     EARERAATGIATLKVGYNRVHGYYIEFGKAQADKAPAHYTRRQTTKNAERYITEELKA
                     FEDKVLSAKERSLMRERALYDGVLDALIEKLGTLKQAAQALAELDVLAALAERAYALN
                     WSRPRLVDEACIAIERGRHPVVEAVRTEPFEPNDLALDDARRMLVITGPNMGGKSTYM
                     RQNALIVLLAHIGSYVPAASATIGAIDRIFTRIGAGDDLARGQSTFMVEMSETANILH
                     NATARSLVLMDEVGRGTSTYDGLALARAAAVHLATANRAFTLFATHYFELTELAGLFD
                     GIANVHLDAVEYRDPQLGEQLVFMHAVKEGPANRSFGLQVAALAGLPKAVIAEAKRTL
                     AALERGEPQGRASAAGSSPQSDLFVKAPDP"
     gene            complement(2628..3710)
                     /locus_tag="VFK00_07140"
     CDS             complement(2628..3710)
                     /locus_tag="VFK00_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HET7592761.1"
                     /translation="MRVKMLAGAIATAVLFPGLTAANPPAPAEAPPQPDEVARGSDPP
                     GQSSASSSISGTIFVDFSDVDPARNGSKTDASGTSLDVTRFYLSFDHAFNDVWSANLT
                     TDANYASATGESEYFVKKAYVQGAFSRMATLRVGAAGTPWIPFVEDWYGYRFVEKLLV
                     DRLRLASSVDWGLHLAGDDGVLDYQVSAVTGAGYRNPTRPDHVDIAARFGVQPVKGLV
                     FAIGGYEGELGAGTSTAPAVHTAHRYDAMAAWNRDGLRLGGEWFKAGNWKNVTTPTAD
                     SASGWSLWGSYDFARASIFARYDRAEPSKQLDPSLRDTYWHTGVAFPVTKDVRIALAY
                     KDEHLRNATGTGVHSREVGGWGEIKW"
     gene            3867..4418
                     /locus_tag="VFK00_07145"
     CDS             3867..4418
                     /locus_tag="VFK00_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HET7592762.1"
                     /translation="MARCGKTRFSVCRIQLVEAVPTDATLRALAESVGEMLRERRQVL
                     ATGESCTGGWIAKLLTDIAGSSGWFEGAAVTYGNDAKQALLGVGAGTLKRHGAVSRNT
                     ALEMVQGVVAGFGASVAVAVTGIAGPGGGTRDKPVGTVWIAWQRRGGQARAEEFRFAG
                     DRDAVRRRTVAAALRGIEKILVS"
     gene            4696..5736
                     /gene="recA"
                     /locus_tag="VFK00_07150"
     CDS             4696..5736
                     /gene="recA"
                     /locus_tag="VFK00_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7592763.1"
                     /translation="MDDNKRRALASALGQIEKQFGKGAVMRMGDRTAEAIDVVSTGSL
                     GLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQAIANCQKNGGTAAFVDAEHALDPSY
                     AEKLGVNVDDLLVSQPDTGEQALEIADMLVRSNAVDMVVVDSVAALTPKAEIEGEMGD
                     NHMGLHARLMSQALRKLTGNIKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFYA
                     SVRLDIRRIGAVKKGDEVIGSQTRVKVVKNKVAPPFRQAEFEILYGEGTSREGELIEM
                     GVANDLVEKSGAWYSYGGERIGQGKENARQYLKDNPQAADELDKTLRAKLLEGTRKGG
                     ATATEDAEEVEA"
     gene            5830..6306
                     /locus_tag="VFK00_07155"
     CDS             5830..6306
                     /locus_tag="VFK00_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET7592764.1"
                     /translation="MKRRLISGSGQPERSAYDRGLSMLARREYSQRELRARLEHAGCD
                     EAESEAALGKLRAQHYQDDARFGEMIVRARVGQGYGPARVRAELRSHGLADSEIRDLI
                     DAAGADWEALAAAQLRKKYGSRTPADRAERGKRAAFLLRRGFPAATVRIVTHSDVD"
     gene            6492..9122
                     /gene="alaS"
                     /locus_tag="VFK00_07160"
     CDS             6492..9122
                     /gene="alaS"
                     /locus_tag="VFK00_07160"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7592765.1"
                     /translation="MKTAEIRARFLEFFRDRGHTIVPSAPLVPANDPTLLFTNSGMVQ
                     FKNVFLGSEKLPYVRAADVQRCLRAGGKHNDLDSVGYTARHHTFFEMLGNWSFGDYFK
                     KEAIGWAWELLTKVFGLPPERLWVTVYHTDDEAFAIWRDAIGVPPERIVRIGDNKGAP
                     FASDNFWQMADTGPCGPCSEIFYDHGPEVAGGPPGSDDEDGDRYIEIWNLVFMQFDRA
                     EDGTLSPLPAPCVDTGMGLERLAAVLQHVHSNYEIDLFQRLIAAAQQVTGCVDRENKS
                     LRVIADHIRACSFLIVDGVLPSNEGRGYVLRRIIRRALRHGWMLGVRGEFFWKMVAPL
                     VAEMGEAYPDLAKKQKFVQEALRAEEQRFGETLEHGMKMFDDIATNSGNAIAGEDAFR
                     LYDTYGFPVDLTADIARERGLTVDMDGFERAMNEQRERARSASRFESKAQLSAELAST
                     LAPTRFIGYETLEAKDCTVLAILRDGRAVEELAPGESAVVILDMTPFYAESGGQVGDT
                     GALEHRAGRFIVTDTQKFGGQFHGHVGEWLGSAPLHVGDKVFARVDAARRQATVLNHS
                     ATHLLHAALRETLGEHVTQKGSLVAPDRLRFDFSHFQPVKPDELRRIEDRVNEQVRRN
                     DPAEISQMAYDEAISTGAMALFGEKYGDEVRVLRMGDFSTELCGGTHVARTGDIGLFK
                     IVSESGVAAGIRRIEAVTGAGAMAHVADEEHRLGEVAGLLSSSAGDVADKLRQVLDRQ
                     KRLERELDALKAKAASSATGDLVAQARDLDGIKLVAARVDGMDAKGLREAVDGLKQKL
                     GDCVVLLASGHDGKAALVGGVHGAALGRIKAGEVVAHVASQIGGKGGGRPDMAQGGGV
                     DSPALDRALDGLGDWLGRAG"
     gene            9357..9581
                     /gene="csrA"
                     /locus_tag="VFK00_07165"
     CDS             9357..9581
                     /gene="csrA"
                     /locus_tag="VFK00_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HET7592766.1"
                     /translation="MLILTRRVGETLMIGDQVTVTVLGVKGNQVRIGITAPKDVAVHR
                     EEIYQRIRQEHGAGALAGADESDERSGTQG"
     gene            9932..10021
                     /locus_tag="VFK00_07170"
     tRNA            9932..10021
                     /locus_tag="VFK00_07170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9966..9968,aa:Ser,seq:gct)
BASE COUNT         1734 a         3249 c         3508 g         1678 t
ORIGIN      
        1 ttcggatcgg gcgccttcac gaagagatcg gattgcggcg agctgccggc ggccgacgct
       61 cgtccctgcg gttcgccgcg ttcgagggcg gccagcgtcc gcttcgcctc ggcgatgact
      121 gccttgggca atcccgccag cgccgccacc tgcaatccga agctgcggtt ggcgggacct
      181 tccttcaccg cgtgcatgaa caccagttgc tcgccgagtt gcgggtcgcg gtattcgacg
      241 gcgtcgaggt gcacgttggc aatgccatcg aacagccccg cgagttcggt cagctcgaag
      301 taatgggtcg cgaacagggt gaatgcgcgg ttcgcggtcg ccaggtggac ggccgccgcg
      361 cgcgccaatg ccaggccgtc ataggtcgag gtgccgcgcc cgacttcgtc catcagcacc
      421 agcgatcgcg cggtcgcgtt gtgcaggata ttggcggtct cgctcatctc gaccatgaag
      481 gtcgattgcc cgcgtgcgag gtcgtcgccg gcgccgatgc gcgtgaagat ccggtcgatc
      541 gcgccaatcg tggcgctggc tgccggcacg taactgccga tgtgcgccag cagcacgatc
      601 agcgcgttct ggcgcatgta ggtcgacttg ccgcccatgt tggggccggt gatcaccagc
      661 atccggcgtg cgtcgtcgag cgcgaggtcg ttcggctcga acggttccgt gcgcacggct
      721 tccaccaccg ggtggcggcc gcgttcgatc gcgatgcagg cttcatccac gaggcgcggg
      781 cgcgaccagt tcagtgcata cgcgcgttcg gcgagcgcgg ccagcacgtc gagttcggcg
      841 agtgcctgcg ccgcctgctt cagggtgccg agcttttcga tcagcgcatc caggacgccg
      901 tcgtacagag cgcgctcgcg catcagcgag cgttccttcg cggacaatac cttgtcctcg
      961 aatgccttca gttcctcggt gatgtagcgc tcggcgttct tggtggtctg gcggcgggtg
     1021 tagtgcgcgg gtgccttgtc ggcctgcgcc ttgccgaact cgatgtagta gccgtgcacg
     1081 cggttgtagc ccactttcag cgtcgcgatg ccggtggcgg cgcgctcgcg ggcttccagt
     1141 tcgacaaggt attggtcggc gtgggtggca agccggcgca gctcgtcgag ttctgcgtcg
     1201 taaccgtcgc cgatggcacc gccttcgcgc agcaaggcgg gcgggcgttc gacgatggcc
     1261 gcctgcaaca attgcaccgt gccctggtgc tcgccgaggc gttccagcag ttcgtgcagc
     1321 aacggtgaat cgagcttcgc cagcgtcgcg tgcaaatccg gcgcggcggc gaggccatcg
     1381 cgcagggtcg caagatcgcg cgggcgcgcc gagcgcagcg caaccctgct gaggatgcgt
     1441 tcgaggtcgc cgatggcgcg tagcttctcg cgcaacgcat cgagcacacc ggattcgatc
     1501 agcgccgcga gcgcctggtg gcgcaagcgg agcgtcgcgt gatcgcgcaa tgggcgatgc
     1561 agccagcgcc gcagtgcgcg ggcgcccatc ggcgtgatgg tggaatccag cacgccgagc
     1621 agggtgtagc gcgtgtcgcc cgacggatgc gtgtcgagtt cgaggttgcg gcgcgtggcc
     1681 gcgtccatcg ccaccgtttc atcggccgac tcgatggcga gtccggtcag gtgcggcagc
     1741 gcggccttct gggtttcttg caggtagccg agcaggcagc ccgccgcggc caccgccagc
     1801 gacaagccgt cggcgccgaa accggacaag tcgcgggtgc cgaagaaccg cgtcagctcg
     1861 cggcgtccgc tggtctcgtc gaaatgccac ggcgggcgca tgcgcagccc gttcgacgcc
     1921 gtgacggccg gcggccacgt cacgtcctcg cacacgagga tctcggcggg cgcgagccgc
     1981 gcgagttcgg cggcaagtgc gtcggcatcg gcgacttcgc acagcaggaa gcgcccgcca
     2041 gcaaggtcga cccaggcgag cccgaatccg gactgtccgg ccgcgatcgc cagcagcagg
     2101 ttgtcccgtc gctccggcag cagcgcctcg tcggtgaggg tgcccggcgt gaccacccgc
     2161 accaccttgc gctccatcgg ccccttgccg cccggatcgc cgacctgctc gcaaatcgca
     2221 accgactccc ccgcgcgcac cagcttggcg aggtacgact ccatcgcgtg gtagggcacg
     2281 cccgccatcg ggatcggcgc accggccgat tcgccgcgct gggtcagcgt gatgtccagc
     2341 agccgcgcgg ccttgcgggc gtcgtcatag aacagctcgt agaaatcccc catccggaag
     2401 aacagcagcg tatgcgggtg ctccgccttg gcggcgaagt actggcgcat cagcggcgtg
     2461 tggcgggcga gttcggcgtg gtccatcgga gcgctgcggg ttcgggcagg cgcgcagttt
     2521 cgcatgcgcc agtcgcagcg cccaagacat ggcctggaat ggattgtcag gtcacgccgc
     2581 agcccagccg cgcgaccgcc ggcatggagc cgatgccgcg gcgcggatca ccatttgatt
     2641 tcgccccatc cgccgacctc ccggctgtgc acgccggtgc cggtggcgtt gcggaggtgc
     2701 tcgtccttgt acgccagcgc aatcctgacg tccttggtca cggggaaggc cacgcccgtg
     2761 tgccagtagg tgtcgcggag tgacggatcg agttgtttgc ttggttcggc gcggtcatat
     2821 cgcgcgaaaa tcgatgcgcg tgcgaagtcg tagctgcccc acagcgacca gcccgaggcg
     2881 gagtcggcgg tgggcgtcgt gacatttttc cagttgccgg ccttgaacca ttccccgccc
     2941 aggcgcaggc cgtcgcggtt ccacgcagcc atggcgtcgt agcggtgcgc cgtatgcacg
     3001 gccggcgccg tcgacgtgcc cgctccgagc tcgccttcat agccgccgat cgcgaacacc
     3061 agccccttga ccggctgcac gccgaagcgg gcggcgatat cgacgtggtc cggccgtgtc
     3121 gggttcctgt atccggcgcc ggtcaccgcc gacacctggt agtcgagcac gccgtcatcg
     3181 ccggcaaggt gcaagcccca gtcgacggaa ctcgcgagcc tcaggcggtc gaccagcaac
     3241 ttttcgacga agcggtagcc ataccagtcc tcgacgaacg ggatccaggg cgtaccggcc
     3301 gcgccgaccc gcagcgtggc catcctggag aacgcgccct gcacataggc cttcttgacg
     3361 aagtactcgg actcgccggt cgcactcgcg tagttggcgt ccgtggtcag gttggccgac
     3421 cagacatcgt tgaacgcgtg gtcgaatgac agatagaacc gcgtcacgtc gaggctggtg
     3481 ccggaggcgt cggtcttgct gccgttgcgt gccgggtcga cgtcggaaaa gtcgacgaag
     3541 atggtgccgc tgatgctgga gctggcggac gattgccccg gcggatccga cccccgcgcg
     3601 acttcgtcgg gttgcggcgg cgcttccgcg ggtgcgggtg gattcgcggc ggtgaggccg
     3661 ggaaacagga cggcggtggc gatggccccc gcaagcatct tcacgcgcac gcagctctcc
     3721 ttgcagaagc gcaagggtag cgggacgggg actatgccgc gagcgcacgg tggcggctcc
     3781 ggagcctgtt tccgtggcag gcacacggcc actttccgcg cccggaatgg ccgggtctgg
     3841 tgccgaggca tgcacccttg gcgcgcatcg cgcgttgcgg taaaactagg ttctccgtct
     3901 gccgaatcca gctcgtggaa gccgttccca ccgatgccac gttgcgcgcg ctcgccgaga
     3961 gcgtgggcga gatgttgcgc gagcgccgcc aggtgctggc aaccggggaa tcctgcaccg
     4021 gtggctggat cgccaagctg ctgaccgaca tcgccggcag ttccgggtgg ttcgaaggcg
     4081 cggccgttac ttacggcaac gacgccaagc aggcattgct cggcgtgggg gcgggaacgc
     4141 tgaagcggca cggcgccgta agccggaaca cggcgctgga gatggtccag ggtgtcgtgg
     4201 cgggcttcgg cgcctcggtc gcggtggcgg tgaccggtat cgccgggcct ggcggcggca
     4261 cgcgggacaa gccggtcgga acggtctgga tcgcctggca gcggcgcggc ggccaggccc
     4321 gagccgagga attccgcttc gcgggcgacc gcgatgcggt gcgccgccgg acggtcgcgg
     4381 cggcacttcg cggtatcgag aagatcctgg tttcctaggg gcagaagctg cgatacaggg
     4441 gtgcaccccc caaggatcgg ggtgacaccc gatcggtgaa ggcggatcgg aacacgcgcg
     4501 aacccacgtg cgcaagcaag ccaaggagtg gctcgtctcg cgcatgggaa accgcttaca
     4561 agccggcgcg gcgtgcgccg ctttccttgc cgatccggta tgaaataatg gcaacgtctc
     4621 cggattcgcg cggtctcaac ccgtgagaac ccgccccgcg atacttcgct ccacgcacac
     4681 cccgaggatt acgcaatgga tgacaacaag cgccgcgcac tggcttccgc gctgggccag
     4741 atcgagaagc agttcggcaa gggcgcggtg atgcgcatgg gcgaccgcac cgccgaggcc
     4801 atcgacgtgg tctcgaccgg ctcgctcggc ctcgacatcg cactgggcgt cggcggtctg
     4861 ccccgcgggc gggtggtcga aatctacgga ccggaatcct cgggcaagac cacgctcacc
     4921 ctgcaggcga tcgccaactg ccagaagaac ggcggcaccg cggcgttcgt cgacgccgag
     4981 cacgcgctcg atccgagcta cgcggaaaaa ctgggtgtca atgtcgacga cctgctggtc
     5041 tcgcagcccg acaccggcga acaggcgctc gaaatcgcgg acatgctggt gcgctcgaac
     5101 gccgttgaca tggtcgtggt cgattccgtg gccgcattga cgcccaaggc cgaaatcgag
     5161 ggcgagatgg gcgacaacca catgggattg cacgcgcgcc tgatgagcca ggcgctgcgc
     5221 aagctgaccg gcaacatcaa gcgttccaac acgctggtga tcttcatcaa ccagatccgc
     5281 atgaagatcg gcgtgatgtt cggcaatccg gaaacgacca ccggcggcaa cgcgctgaag
     5341 ttctacgcgt cggtgcgcct cgacatccgc cggatcggcg cggtcaagaa gggcgacgag
     5401 gtgatcggct cgcagacccg cgtgaaagtc gtcaagaaca aggtggcgcc gccgttccgc
     5461 caggccgaat tcgagatcct gtacggcgag ggcacctcgc gcgaaggcga actgatcgag
     5521 atgggcgtcg ccaacgacct ggtcgagaag tccggcgcct ggtacagcta cggcggcgag
     5581 cggatcgggc agggcaagga aaacgcccgc cagtacctca aggacaaccc gcaagctgcg
     5641 gacgagctcg acaagacgtt gcgtgcgaag ctgctcgaag gcacccgcaa gggcggcgcc
     5701 accgcgaccg aggatgccga ggaagtcgag gcataacgcc aagatttgtt tcacgtagga
     5761 gcggcccaag gccgcgacct gggtggttac gccggatcgc ggccaggggc tgctcctaca
     5821 attgtgcgaa tgaaacgccg actgatttcc ggttcgggcc agcccgagcg cagcgcctac
     5881 gaccgcgggc tctcgatgct cgcccgccgc gagtattccc agcgcgagct gcgcgcgcgg
     5941 ctggagcacg ccggttgtga tgaagccgag tccgaagccg cactcggcaa actgcgtgcg
     6001 cagcactatc aggatgacgc gcgcttcggc gaaatgatcg tgcgggcacg ggtggggcag
     6061 ggctacggac cagcgcgggt ccgggcagaa ctccgttcgc atggactcgc cgattcggag
     6121 atccgcgacc tcatcgatgc cgcgggtgcg gactgggaag ccctggccgc cgcccagctt
     6181 cgcaagaagt acggcagccg gacgcccgcc gatcgtgccg aacgcggcaa gcgcgcggcc
     6241 ttcctgttgc gtcgcggctt ccccgctgcc accgttagaa ttgtcaccca ttccgacgtc
     6301 gactgacttt gtttttgcga tcatccctga tcgcggcgtc gcgggactgt gtcgcagggg
     6361 aagggttacg gtggacgtgg gcaccagaac ggccagccat ggcctgactt tcaacaacag
     6421 cggctccgaa tgaagcgtct ttggtacgct tcgaaggttg ctaccatcca tgaaatttta
     6481 gacgtaaccg catgaaaact gcagagattc gcgcgcgctt cctcgagttc ttccgcgacc
     6541 gtggccacac gatcgtgccg tccgcaccgt tggtgccggc caacgatccg accctgcttt
     6601 tcaccaactc ggggatggtg cagttcaaga acgtgttcct cggcagcgag aagctgccgt
     6661 acgtgcgcgc ggcggacgtg cagcgctgcc tgcgcgccgg cggcaagcac aacgacctcg
     6721 attcggtcgg ctacaccgcg cgccaccata ccttcttcga aatgctcggc aactggtcgt
     6781 tcggcgatta tttcaagaag gaagcgatcg gatgggcctg ggagctgctg acgaaggtgt
     6841 tcgggctgcc gccggagcgc ctctgggtca cggtctacca cacggatgac gaggcctttg
     6901 ccatctggcg cgacgcaatc ggcgtaccgc ccgaacgcat cgtccgcatc ggcgacaaca
     6961 agggcgcgcc gttcgcctcg gacaatttct ggcagatggc ggacaccggt ccctgcggtc
     7021 cgtgcagcga aatcttttac gaccatggcc ccgaggtcgc gggcggtccg cctggctccg
     7081 acgacgaaga cggcgaccgt tacatcgaga tctggaatct cgtgttcatg cagttcgacc
     7141 gtgccgagga cggcacgctg tcgccgctcc ccgcgccatg cgtcgatacc ggcatggggc
     7201 tcgaacgcct cgccgcagtc ctccagcacg tgcactccaa ttacgagatc gacctgttcc
     7261 agcggctgat cgccgcggcc cagcaagtca ccggctgcgt ggaccgcgaa aacaagtcgc
     7321 tgcgcgtgat cgcagaccac atccgcgcct gctcgttcct gattgtggac ggcgtgttgc
     7381 catccaacga aggccgcggc tacgtcctgc gccggatcat ccgccgcgcg ctgcggcacg
     7441 gctggatgct gggcgtgcgc ggtgagttct tctggaagat ggtcgcaccg ctggtcgcgg
     7501 agatgggaga ggcctatccc gatctagcga agaagcagaa gttcgtgcag gaggcactgc
     7561 gagcggaaga gcagcgcttc ggcgagacgc tggaacacgg catgaagatg ttcgacgata
     7621 tcgccacgaa ctcggggaac gccattgcgg gcgaagatgc attccgcctg tacgacacct
     7681 acggctttcc ggtggacctg accgccgata tcgcgcgcga gcgcggattg accgtggaca
     7741 tggacggctt cgagcgcgcc atgaacgaac agcgcgaacg cgcgcgctcc gccagcaggt
     7801 tcgagagcaa ggcacagttg tccgccgagc tcgcgagcac cctcgccccc acccgattca
     7861 tcggttacga gacgctggaa gcgaaggatt gcacggtgct ggcgatcctg cgcgacggcc
     7921 gcgcggtgga ggaactcgcg cccggcgaat ccgcggtggt gatcctggac atgacgccgt
     7981 tctacgccga atccggcggg caggtcggcg ataccggtgc gctggaacat cgtgccggac
     8041 gcttcatcgt caccgatacg cagaagttcg gcggccaatt ccacggccac gtgggcgagt
     8101 ggctgggcag tgcgccactg cacgtcgggg acaaggtgtt cgcgcgggtc gatgccgcgc
     8161 gccgtcaggc cacggtactg aaccactcgg ccacgcacct gctgcatgcg gcgctgcgcg
     8221 agacgctggg tgaacacgtg acgcagaaag gctcgctggt cgcgcccgat cgcctgcgct
     8281 tcgacttctc gcatttccag ccggtgaagc ccgacgagct gcggcggatc gaggatcgag
     8341 tcaatgagca ggtccgccgc aatgatccgg ccgaaatcag ccagatggcc tacgacgagg
     8401 cgatctccac cggtgcaatg gcgctgttcg gcgagaagta cggcgacgaa gtgcgcgtgc
     8461 tgcggatggg ggatttctcg acggaattgt gcggcggtac ccacgtcgcg cgcaccggcg
     8521 acatcgggct gttcaagatc gtgtcggaat caggcgtcgc cgccggcatc cgccgcatcg
     8581 aagcggtgac cggtgcgggc gcgatggctc acgtcgccga cgaagaacat cgcctgggcg
     8641 aggtcgcggg cctgctttca tccagtgccg gcgacgtcgc cgacaagttg cgccaggtgc
     8701 tcgacaggca aaagaggctg gagcgcgaac tcgatgcact gaaggccaag gcggccagtt
     8761 ccgccaccgg cgatctggtc gcgcaagcgc gggacctcga cggcatcaag ctggtcgccg
     8821 cgcgagtcga cggcatggat gccaagggct tgcgcgaagc ggtcgatggc ttgaagcaga
     8881 agctcggcga ttgcgtggtg ctgctcgcat ccgggcacga cggcaaggcg gcgctggtgg
     8941 gtggcgtgca cggcgcggcg ctcggccgga tcaaggccgg cgaggtcgtg gcgcatgttg
     9001 ccagccagat cggcggcaag ggcggtggtc gtccggacat ggcgcagggc gggggcgtcg
     9061 actcgccggc gctcgatcgc gcgctggacg gattggggga ctggctgggg cgcgcgggct
     9121 gagtcgaccc tgccggaagt catggtggtt tcgttgcgcg aataacactc gttcctatat
     9181 gctcgcggag caacgatcgc ccctgcaccg gtctagtgcg ggtgatggag gaacctgaac
     9241 gtcgaaacgc ggcctgtgtc cgttcgcgat cgcccagtca cgccgtacgt gcaacccgga
     9301 ggggaacgcg ctatgcggtt tcgggtccaa aggactcgaa tacatggagt tcagtcatgt
     9361 tgattttgac tcgccgtgtc ggcgagacct tgatgatcgg cgaccaggtc accgtcacgg
     9421 tgttgggggt caagggtaac caggtgcgca tcggtattac cgcacccaag gatgtcgccg
     9481 ttcaccgtga ggaaatctac cagcgcatcc gacaggagca tggtgccggc gcactggctg
     9541 gtgccgatga gtccgacgag cgaagcggta ctcagggttg atccgggggt tcttcgactt
     9601 taccgaagaa cgattcgcgt ttattctcga ccaccggttt gccgcggccg acaggtatcg
     9661 tgtcgccgcc atttgccgcg cgtgaagttt gcctggaaga cacccggaga gatgcccgag
     9721 aggccgaagg ggctcccctg ccaaggggat atcgggtcaa aagcctgatc gagggttcga
     9781 caaaatcgcc gagagcgatt ttagacggcc caaaggccgg ccccgagcgc aacgaggggt
     9841 gagcatcagg gatgatgcaa acttatcccg aacgggcggt cagcgtgcct cgacctccgt
     9901 tgttacactt ctgctttgca gtcaaaaatc cggagagatg cccgagaggc cgaaggggct
     9961 cccctgctaa gggagtatcg ggtcaaaagc ctgatcgagg gttcgaatcc ctctctctcc
    10021 gccggatacg caaaacgccc cgaacggggc gttttgcgta tctggcggag agagatgtgt
    10081 gagcgaaccc tccggtgact cggcgcatcc tgcgcctcac ccttcgggcc agcggctttg
    10141 ccgctgttcg ctccggcatc ctgcctccg
//
LOCUS       DASSNH010000193         5360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1014942, whole genome shotgun sequence.
ACCESSION   DASSNH010000193 DASSNH010000000
VERSION     DASSNH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014942"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VFK00_07175"
     CDS             complement(<1..123)
                     /locus_tag="VFK00_07175"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483319.1"
                     /note="catalyzes the hydrolysis of pyrophosphate to
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HET7592767.1"
                     /translation="MGLDLVPAGRDVPDDINVVIEIPKDAEPVKYEVDKASGAIF"
     gene            complement(204..2333)
                     /locus_tag="VFK00_07180"
     CDS             complement(204..2333)
                     /locus_tag="VFK00_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7592768.1"
                     /translation="MKAIWPALALGIIVSGTALANPSPKNNEPAPAASSADPYLWLEP
                     SHSQRAMEWVHAQNAITQKAFMESPEFDKTRGRILEVLDSEARIPYVYRMGDYLYNFW
                     QDKANPRGIWRRTTLAEYEKADPKWETVLDIDALNKAEDKKWVFKGARCMKPDYTHCL
                     VSLSPGGGDAVEVREFDLPSKTFVDGGFFLPVAKTEVDWIDADHIYVGTDFGPGSMTK
                     SSYPRIVKEWTRGTPLSAATVVYEGKPTDLGVGAYHDRTKGYERDFVSVQKDFFHGDM
                     YLRSGGKLAKVDVPEDANADVHRQWLLVQTKSPWTTGGRTWPAGALIAMKFDDFMAGK
                     RDFAALFTPDAHTALAGYSWTQNHIILDVLDDVKSRLDVLTPPASGNGEWQHQALPGA
                     PKFSTIAVVDTDPDHSDEYWLTVTGFLDPPSLERGVLGSEPAKKIKQEPAFFDASKFE
                     VSQHFVTSKDGTRVPYFQIAPKDMKLDGSNRTLEYGYGGFEVSLLPHYSGSIGRAWLE
                     RGGVYVVANIRGGGEYGPSWHKAALKANRPKAYQDFAAVAEDLIKRGVTSPQHLGAEG
                     GSNGGLLMGNMLTEYPQLYGAIACEVPLLDMKRYTHLSAGASWMAEYGDPDDPEQWAF
                     IKAFSPYQNVEPNMHYPDVLFYTTTSDDRVGPAQARKMAAKMEAMGYENVWFYENTEG
                     GHGAGADNQQSATMHALAYDFLWDKLQ"
     gene            2803..3714
                     /locus_tag="VFK00_07185"
     CDS             2803..3714
                     /locus_tag="VFK00_07185"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7592769.1"
                     /translation="MFIGALLFSSPLLAVVILPTNAVLTSNVAYGSDPLQKLDVYAPK
                     GVHGAPIIFMVHGGGWWRGDKAFPGVVQNKINHFLPKGIIFVSVNYRLSPQVGVLDEA
                     KDVARALIYVQAHATQWGGDPNRVVVMGHSAGAHLTSLVTTAADIHKGAKRWLGNIEL
                     DSAALNVVQIMKAPHPRSLYDPIFGANPDLWAEASPTLRITAAPPPMLMVCSIAHPGS
                     CRQTEEFVDAAYGMAVEYQTGLDHGSIDTTLGQSNQLTDAVDAFLCSLGVGCGESGAR
                     STDRSTKTPPRVLCSLRKHSAKPCPKR"
     gene            complement(3848..>5360)
                     /locus_tag="VFK00_07190"
     CDS             complement(3848..>5360)
                     /locus_tag="VFK00_07190"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7592770.1"
                     /translation="HTWTISYRTNRQVGFFPEHDELFWNVNGTGWIFPVDRVGASVHL
                     PRAVPAAKLEAYGYTGSQDSREQALAASVHDGGADYITTHPLGSNEGLSIVLEFPKGV
                     VTAPSTAQRWRWWLADNFNILLAVIALVGLWIYYVQVWRRKGRDPAAGPLVAEYEPPD
                     GDSAAALRYVQRMGYDDTCFTAGILGVAAKGGLDIREDQDKTYIATRRDGGKAVLSAD
                     EEALRDALFAAGAEIEFKQSEHSRVQAARSAHQKALKAAFYSKYFLTNSLWLLPGIAI
                     TVLSAWLASLGGAEGTGFLLAWIGFWTVGVVLLFNRAAHGGRGAVGGWIMFVFFAAAE
                     LIAIVALGTQAGYALIPLCIALVVTNLLFYQWLKAPTQLGATLLDRIRGFRWYLGVAE
                     KQELDARYKPESRPDLFAAYLPYALALGVGNAWAERFSSALTPAQMEQARPSWYSGTG
                     AGGFTGASFTTFASSMTSGFGSAIASASVAPGSSSGSGGSSGGGGGGGGGGGW"
BASE COUNT          890 a         1749 c         1734 g          987 t
ORIGIN      
        1 gaagatcgcg ccggaggcct tgtccacctc gtacttgacg ggttcggcgt ccttggggat
       61 ttcgatgacg acgttgatgt cgtccggcac gtcgcggccg gcggggacga gatccaggcc
      121 catgggagtg tccttgcgaa acccgggatt ttacgacggg cacaggaaaa ccggcggcga
      181 ccccggtttt cccccggttt tccttactgc aatttatccc acaggaagtc gtaggccagc
      241 gcgtgcatgg tcgccgactg ctggttgtcc gcgccggcac cgtggccgcc ttccgtgttt
      301 tcgtagaacc agacgttctc gtagcccatg gcttccatct tcgcggccat cttgcgcgcc
      361 tgcgccgggc cgacgcggtc gtcgctggtc gtggtgtaga acagcacgtc cggatagtgc
      421 atgtttggct cgacgttctg gtacggcgag aaggccttga tgaacgccca ttgctcggga
      481 tcgtcgggat cgccgtattc ggccatccac gatgcgcccg ccgacaggtg cgtgtagcgc
      541 ttcatgtcga gcagcggcac ttcgcacgcg atcgcaccgt agagctgcgg atattcggtc
      601 agcatgttgc ccatcagcag gccgccgttg ctgccgcctt cggcaccgag gtgctgcggc
      661 gaggtgacgc cgcgcttgat caggtcttcc gcgaccgcgg cgaagtcctg gtacgccttc
      721 ggacggttgg ccttgagcgc agccttgtgc cacgatgggc cgtattcgcc gccgccacgg
      781 atgttggcga ccacgtacac cccgccgcgt tcgagccacg cgcggccgat cgagccggag
      841 tagtgcggca gcagcgacac ctcgaagccg ccgtagccgt attccagcgt gcggttgctg
      901 ccgtcgagct tcatgtcctt cggagcgatc tggaaatacg gcacgcgggt gccgtccttc
      961 gaggtcacga agtgctggct cacctcgaat ttcgaagcgt cgaagaacgc cggttcctgc
     1021 ttgatcttct tcgcgggttc gctgcccagc accccgcgtt cgagcgacgg cggatcgagg
     1081 aacccggtga ccgtgagcca gtattcgtcg gaatggtcgg gatccgtatc gacgaccgcg
     1141 atggtgctga acttcggcgc gcccggaagc gcctggtgtt gccattcgcc gttgccggag
     1201 gccggcggcg tcagcacgtc gagacggctt ttcacgtcgt ccagcacgtc caggatgatg
     1261 tggttctgcg tccacgagta gcccgcgagc gcggtgtgcg catcgggcgt gaacagcgcg
     1321 gcgaaatcgc gcttgccggc catgaaatca tcgaacttca tcgcgatcag cgcgcccgcc
     1381 ggccaggtcc tgccgccggt cgtccagggc gatttggtct gcaccagcag ccattggcga
     1441 tgcacgtcgg cattggcgtc ttccggcacg tcgaccttcg cgagcttgcc gccgctgcgc
     1501 aggtacatgt cgccgtggaa gaagtccttc tgcaccgaaa cgaagtcgcg ctcgtagcct
     1561 ttggtgcggt cgtggtaggc gccgacgccg agatcggtcg gcttgccttc gtacacgacg
     1621 gtggccgccg acagcggcgt gccgcgcgtc cattccttga cgatccgcgg atagctcgac
     1681 ttggtcatgg agccgggccc gaagtcggtg ccgacgtaga tgtgatcggc gtcgatccag
     1741 tcgacctcgg ttttcgcgac cggcaggaag aagccgccgt ccacgaaggt tttcgagggc
     1801 aggtcgaact cgcgtacctc caccgcatcg ccgccgccgg gcgacagcga caccaggcaa
     1861 tgggtgtagt cgggcttcat gcagcgtgcg cccttgaaca cccacttttt gtcttcggcc
     1921 ttgttcagcg cgtcgatgtc gaggaccgtt tcccacttgg gatcggcctt ctcgtattcg
     1981 gccagcgtgg tgcgccgcca gatcccgcgc ggattggcct tgtcctgcca gaagttgtac
     2041 aggtagtcgc ccatccggta gacgtagggg atgcgcgcct ccgagtcgag cacttcgagg
     2101 atcctgccgc gggtcttgtc gaactccggc gattccatga acgctttttg ggtgatggcg
     2161 ttctgcgcgt gcacccactc catcgcacgt tggctgtggc tgggctcgag ccagagatac
     2221 gggtccgcgg acgatgccgc cggcgccggt tcattgttct ttggggaagg attagccaat
     2281 gccgtgcccg aaacgatgat gcccaatgcc agtgccggcc agatcgcctt catcgtcgct
     2341 gcctcgtgtg ggattccggc catgttgaac ggattggcgc gacggcgcac gtgacgaaag
     2401 gcatggcatg ccgacaggga tcaaccgttc accagcagcc accgccgccg tccgatgcgt
     2461 cgccgacccg gaatgccacc aggggtgtgc ctgctcgatc tgcgcggtcg tcagtgccta
     2521 ccagaaccgt cccggcgctg ccgtgagctg cggacaactt catccacgtc acggcagatg
     2581 cgccgagttc gacacgtccg atgtctgttg gcgcatcgag tgaatcgagc gatcgcagtg
     2641 atattgaggc agccgtcact ttcaccagct tcccccgcag ggatactcgg ttcgcgacag
     2701 cattcctgtt gtcttgaccg accatccttc cagtgaagag ttgaaatgac ccacgctact
     2761 ttcccccgca acgcccgcaa gacgcgcacc ttcgcagcgt tgttgttcat cggcgcattg
     2821 ttgttttcga gtcccctcct ggccgtggtc atccttccga ccaatgccgt cctgacttcc
     2881 aacgtggcct acggaagtga tccattgcag aagctggacg tgtacgcccc gaagggggtc
     2941 cacggggcgc ccatcatctt catggtccat ggcggtggct ggtggcgcgg cgacaaggcg
     3001 ttccccggtg tcgtgcagaa caagatcaac cacttcctgc ccaaggggat catcttcgtt
     3061 tccgtcaact accggctttc cccgcaagta ggcgtgctgg atgaggcgaa ggacgtggcg
     3121 cgcgcattga tctacgtgca ggcgcacgcg acacaatggg gaggtgatcc gaatcgcgtg
     3181 gtggtgatgg gacattccgc gggcgcgcac ctgacaagcc tggttacgac cgcggccgac
     3241 atccacaagg gcgcgaaacg gtggctcgga aatatcgagc tagacagcgc ggcgctcaac
     3301 gtggtgcaaa tcatgaaagc cccgcacccg cgttcgctgt atgaccccat tttcggcgcc
     3361 aacccggacc tctgggcgga agcctcgccg accttgcgca ttaccgcggc gcccccgccg
     3421 atgctgatgg tgtgctccat cgcacacccc ggatcctgcc ggcagacgga ggagttcgtc
     3481 gatgcggcct atggcatggc ggtggagtac caaaccggcc tggatcatgg atccatcgac
     3541 accactctcg gccagtcgaa ccagctgacg gacgcagtcg acgctttcct ctgctcgctt
     3601 ggcgtgggtt gtggcgagtc tggagcaaga agtacggatc ggtcgacgaa gacgccgccc
     3661 cgggttcttt gctccttgcg gaagcattcg gcaaagccat gcccgaaacg atgatgcccg
     3721 atgccagcga cggccagacc gccgttgctg gctcacgggg gcaactccga cggtggtgag
     3781 cggatcgtcg gggcggtgca ggtgacggaa agcctggcgc gtccaaaggc caggctccgc
     3841 acctctctca ccagccgcct ccgccgccac cgccgccgcc gccgcccgag ctcccgcccg
     3901 agcccgaact ggagcccggc gccaccgacg ccgaggcgat tgcgctgccg aaccccgagg
     3961 tcatgctgct ggcgaatgtc gtgaagctcg cgccggtgaa gccgccggcg cccgtgcccg
     4021 aataccacga aggtcgcgcc tgctccatct gcgcaggcgt cagcgcactg gaaaagcgtt
     4081 cggcccaggc gttgccgacg cccagcgcga gggcatacgg aaggtaggcg gcgaacaggt
     4141 ccggtcgcga ttcgggcttg tagcgggcgt cgagttcctg tttctcggcg acaccgagat
     4201 accagcggaa accgcggatg cggtcgagca atgtcgcgcc caattgggtg ggcgccttca
     4261 gccactggta gaacaacaga ttcgtcacga ccagggcgat gcacagcgga atcagcgcat
     4321 acccggcctg tgtccccagc gccacgatcg cgatgagctc ggccgccgca aagaagacga
     4381 acatgatcca gccgccgacc gcgccgcgac cgccatgcgc cgccctgttg aacagcagga
     4441 ccacgcccac cgtccagaag ccgatccacg cgagcaggaa gccggtccct tcggcgccgc
     4501 cgaggctcgc cagccatgcg ctcaagacgg tgattgcgat acccggcagc agccacaggc
     4561 tgttggtgag gaaatacttg ctgtagaacg ccgccttcag cgctttctgg tgggcgctgc
     4621 gcgcggcctg tacgcggctg tgctcgctct gcttgaactc gatctcggcg ccggcggcga
     4681 acagcgcgtc ccggagtgcc tcctcgtcgg cggacaagac ggccttgccg ccatcgcgcc
     4741 gggtggcaat gtaggtcttg tcctggtcct cgcggatgtc gagcccgcct ttggcggcaa
     4801 cgcccaggat gcctgcggtg aaacaggtgt cgtcgtagcc catgcgctgg acgtaacgga
     4861 gcgcggcggc ggagtcgcca tccggcggtt cgtattcggc gaccaacggc cccgccgcgg
     4921 gatcccggcc cttgcgccgc cagacctgca cgtaatagat ccacaagccg accagcgcga
     4981 tcacggccag caggatgttg aagttgtctg cgagccacca gcgccaccgc tgcgcggtcg
     5041 aaggcgcggt gaccacgccc ttcgggaact cgagcacgat gctgagaccc tcgttggacc
     5101 cgagcgggtg cgtggtgatg tagtccgcgc cgccgtcatg gacgctcgcc gccagcgcct
     5161 gctcgcgcga atcctggctg ccggtgtagc cgtaggcctc gagtttcgcc gccggcaccg
     5221 cgcgtggcag gtgcacgctc gcgcccacgc ggtccaccgg aaagatccag ccggtgccgt
     5281 tgacgttcca gaaaagttcg tcgtgctcgg ggaaaaaccc gacctggcgg ttggtgcggt
     5341 agctgatcgt ccaggtgtgc
//
LOCUS       DASSNH010000194         8561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1302125, whole genome shotgun sequence.
ACCESSION   DASSNH010000194 DASSNH010000000
VERSION     DASSNH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302125"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..3107
                     /locus_tag="VFK00_07195"
     CDS             504..3107
                     /locus_tag="VFK00_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET7592771.1"
                     /translation="MRILKRLALIALIGIACLVVLGLAAGGIAYWLIEPRVPSVASLK
                     DVQMQVPLRVLSADGKLIAEFGETRRMPVDIRNVPDKLRNAVLAAEDADFYHHSGIDF
                     TSTFRAAVEVALHRGEKVQGGSTITQQVARNFFLSPQKTYTRKITEILTSFRIENELS
                     KDQILQLYLNKMFLGHRSYGVAAAAQYYYGKKIDQLTLAQCAAIASTFQLPSVVNPLD
                     HKERLIARRNWVLGQMLSHGFIQRAQYEEAVAAPDDASPHEPPVEVDAPYLAEMVRQQ
                     AMQKLGNQALTDGYVIHTTLDSRLQALANHALRSRLEEYDHRHGYRGPEAHVDLPQPA
                     TPTTLAEAVSGYGDLSDLLPAIVTASDAEAATVYVQGTGTVTLPTDDMKWALGYARKA
                     KTGGKGAASILAPGDIIRVRSIHGENDVLAAAAPAEPADPKAEADKGNTNRDDTTKGK
                     SGKAPEEAAADKSRWALEQVPAAQAAMVVLSPDDGAVQALVGGFNFRTSKFNRATQTQ
                     RQPGSSFKPFIYSAAFSRGYTAASIINDAPIALPDPSKPGGLWTPQNDDGKFRGPMRL
                     REALAQSVNLVAIRLLDAVGVRYVREYASRFGFPLDAIPPNLSMALGTASVSPMAMAR
                     AYAVFANGGFLVNPYYIAEIDNRDGKAIYKADPVRACRNCEARLLDEGKTTAAPAQPA
                     SAAGDPGALGDTANALATGESNGPLLAPRVIDPRNDFLTVSLLHSVIDHGTGYAVKQL
                     GRSDLAGKTGSTNDHHDGWFCGFNGDMVATVWVGFDDYRSLGRGEFGAKTALPIWMAF
                     MGPALEGKPESALAMPPGIVTATIDRSTGLPAPPGDTNTMSEIFRVENLDRLRAEAKA
                     QQNKAPAYDIF"
     gene            3344..3979
                     /locus_tag="VFK00_07200"
     CDS             3344..3979
                     /locus_tag="VFK00_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592772.1"
                     /translation="MQKTAEFHRIHAEGRAHQLRRRVAIEAARLISEQGIRDYRMAKR
                     KAAQRIGVRDEGFLPRNREIEDALREHQRLFRGDEQARAQQFRLETARDAMKFLSRFE
                     PRLVGSVLEGTSDVHSPVSLHVFDDTPEAVARFLHDHRIAFETRDRTVRLDRTRSGEF
                     PVLLFEADGVPVDVTIFPRDALRQAPLDRIDERPLQRASLAAVEMMLAGVG"
     gene            complement(4134..5420)
                     /locus_tag="VFK00_07205"
     CDS             complement(4134..5420)
                     /locus_tag="VFK00_07205"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7592773.1"
                     /translation="MPNQNVTLTAADHSVSLPLVEGSQGPSCVDISSLYRETGHFTFD
                     PGFAATASCKSAITFIDGEKGVLLYRGYPIEQLAERSSFLEVAYLLLEGELPTKAEMT
                     KFEHSVTHHTMMHESLKNFLHGFHYDAHPMAMLCAAIASLSAFYHDTLDIQDPDQRRL
                     AAVRLLAKMPTIAAASYRYSIGWPIRYPRNSLRYVDRFLHMMFEVPSEPLELNPVVAE
                     ALDLLFILHADHEQNASTSTVRLVGSTGANPYASVAAGIAALWGPAHGGANEAVLKML
                     TEIGDEKNVGKAIDRAKDKESGFRLMGFGHRVYKNFDPRARIIRAKCHEVLDKLGVHD
                     PLLDVAMKLEEAALKDDYFIEHKLYPNVDFYSGIIYKALRIPTEMFTVMFAIARTAGW
                     IAHWIEQHEMPGGRIGRPRQVYVGATPREYVAVEKR"
     gene            complement(5799..6047)
                     /locus_tag="VFK00_07210"
     CDS             complement(5799..6047)
                     /locus_tag="VFK00_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET7592774.1"
                     /translation="MKADTHPNYQPVVFQDAASDFAFLTRSTMTSSETIQWEDGQEYP
                     LVKIDISSASHPFYTGKQKVMDTGGRIDKFKRRYTAGK"
     gene            complement(6128..8299)
                     /gene="recG"
                     /locus_tag="VFK00_07215"
     CDS             complement(6128..8299)
                     /gene="recG"
                     /locus_tag="VFK00_07215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7592775.1"
                     /translation="MTSPADTRIERSPDPGLAPATLLPGVGPALAQTLRKLGIERVQD
                     LWFHLPLRYEDRTRITAIRDLVAGESAQVEGVVEAVESGFRYRPQLRVAIGDASRDTL
                     VLRFFHFRRQQAQQFSPGTRLRCYGAIRHGQHGLEMVHPQYRVLRGEAAVETNLTPVY
                     PVTEGLGQQRLRGVIDKALARLPDAAVLELVPAGMLASLGMMPLRDALLGVHRPPPDA
                     DVAALLAGRHPAQRRLAFEELLAHHLSLKQRRARVRRQRAPALPADPELESRFLEALP
                     FSLTRAQRRVADEVGRDLGKRVPMLRLVQGDVGSGKTVVAALAALRAIADGRQTALMA
                     PTELLAEQHLRSFREWLEPLGIEVVWLAGKVAGRARTKALAQIAAGAPVVVGTHALMQ
                     EGVEFHGLALAIVDEQHRFGVQQRMALRDKGVVAPQAPQARGIDAGRKHTREGFLPGY
                     APHQLVLTATPIPRTLAMSAYADLDVSVLDELPPGRTPVRTVAIANTRREEVIERIHA
                     ACAAGRQVYWVCTLIEDGEQLQAQAAEVAHAQLSAALPQVEVGLVHGRLKAREKQAAM
                     DAFKDGRCAVLVATTVVEVGVDVPNASLMVIENAERLGLAQLHQLRGRVGRGDVASSC
                     VLLYQPPLSALARQRLETLRETSDGFVIAETDLRLRGPGELLGTRQTGDMAFRIADIV
                     RDAPLMPEVQRVGAAMLRSHPALARRLADRWIGGAARFAGA"
     gene            complement(8328..>8561)
                     /locus_tag="VFK00_07220"
     CDS             complement(8328..>8561)
                     /locus_tag="VFK00_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HET7592776.1"
                     /translation="RVFENLKAVLGAANATFDDVVRVAIYMTDLGNFSAVNEVMKRYF
                     NEPYPARSTIGVAALPRGAAVEVDVIAVTGASA"
BASE COUNT         1452 a         2981 c         2771 g         1357 t
ORIGIN      
        1 cggtcgaact gatgtcgacg ccgatcaggg gcgcagcact ggttgtcagt attcccactg
       61 gagttcccct tccccatccc cgcatgcgct gaaaccctgc gcgcctgcat tagatacgaa
      121 agttaacgaa atatcaataa ccttgcaaag tgcatacaag cccatgaagt tccgcagctt
      181 tcggccttag atgatgcact ccaacgtcac ttttggccgt gcatgcgtca caaatccgtc
      241 agctcgtttt cagggcggtg cgggcgttgc gacggacccc tcgaaaacga cccttccggt
      301 gggcgatcga ctccggtcga aggggccttg caccaccgca ggtcgcgcat gccgccatgt
      361 gacccgcatg gaaccccggc gtggcatgag cctctatact ttcgtgtccg ctccgcacgg
      421 gcagcgaacc tcgtgccgac attccaagca acatcggccg tataccgcgc aacgcggccg
      481 gccacgccgc aaccggaaag cgcatgagaa tcctgaaacg actggcgctg atcgcactga
      541 tcggcattgc gtgcctggtg gtgctgggcc tcgccgcagg cggcatcgcc tactggctga
      601 tcgagccgag ggttccgtcg gtcgcctcgc tcaaggacgt gcagatgcag gtgccgctgc
      661 gggtgctctc cgcggatggc aagctgatcg ccgaattcgg ggagacgcgg cgcatgccgg
      721 tcgacatcag gaacgtcccc gacaagctcc ggaacgcggt gctggccgcc gaggacgcgg
      781 acttctacca ccactccggc atcgacttca cctccacttt ccgcgccgcc gtcgaggtcg
      841 cgttgcaccg gggcgaaaaa gtccagggcg gctcgaccat cacccagcag gtcgcccgca
      901 atttcttcct gagcccgcag aagacctaca cccgcaagat caccgaaatc ctcacctcgt
      961 tccgcatcga gaacgagctg agcaaggacc agatcctgca gctctacctc aacaagatgt
     1021 tcctgggaca caggtcctac ggcgtcgccg cggccgcgca gtattactac ggcaagaaga
     1081 tcgaccagtt gaccctcgcc caatgcgcgg cgatcgcctc gaccttccag ttgccctcgg
     1141 tggtcaatcc gctcgaccac aaggaacggc tgatcgcgcg ccgcaactgg gtgctcggcc
     1201 agatgctctc gcacgggttc atccagcgcg cgcaatacga ggaagccgtc gcggcaccgg
     1261 acgacgcctc gccgcacgag ccgcccgtcg aagtcgatgc gccctacctc gccgaaatgg
     1321 ttcgccaaca ggcgatgcag aagctcggca accaggcgct cacggacggc tacgtgatcc
     1381 acacgaccct cgacagccgg ctccaggcgc tcgccaacca cgccctgcgc agccgcctcg
     1441 aggagtacga ccatcggcac ggctaccgcg gcccggaagc gcacgtcgac ctgccgcaac
     1501 cggcgacgcc cacgacgctc gccgaggcgg tgtccggcta cggcgacctc agcgacctgc
     1561 tgcccgcgat cgtcaccgcc agcgacgccg aggccgcgac cgtctacgtg cagggcaccg
     1621 gcaccgtcac cttgcccact gacgacatga aatgggcact cggctacgcg cgcaaggcga
     1681 agaccggcgg caagggcgcg gcatcaatcc tcgcgcccgg cgacatcatc agggtgcgga
     1741 gcatccatgg cgagaacgat gtgctcgccg ccgcggcccc cgccgaacct gccgacccga
     1801 aggccgaggc ggacaagggc aacacgaacc gggacgacac gaccaagggc aagagcggca
     1861 aggccccgga ggaagcggct gccgacaaat cccgctgggc cctcgaacag gttccggctg
     1921 cgcaggccgc gatggtcgtg ctctcgcccg acgacggtgc cgtgcaggcg ctggtcggcg
     1981 gcttcaactt ccgcaccagc aagttcaacc gcgcaaccca gacccagcgc cagcccggct
     2041 ccagcttcaa gcccttcatc tattctgccg cgttcagccg cggctatacg gccgcctcga
     2101 tcatcaatga cgctccgatc gcgctgccgg atccctccaa gcccggcggg ctgtggacgc
     2161 cgcagaacga cgacggcaag ttccgcggcc cgatgcggct gcgggaagca ctggcgcaat
     2221 cggtgaacct cgtggcgatc cggctgctcg atgcagtcgg cgtccgctac gtgcgcgagt
     2281 acgcctcgcg cttcggattc ccgctcgacg cgataccccc gaacctttcg atggcgctcg
     2341 gcaccgcatc ggtttccccg atggcgatgg cgcgcgccta cgcggtgttc gccaacggcg
     2401 gcttcctggt caacccctac tacatcgccg agatcgacaa ccgcgacggc aaggccatct
     2461 acaaggccga tcccgtccgc gcctgccgga actgcgaggc gcggctgctg gacgaaggca
     2521 agaccaccgc cgcgcctgca cagcccgctt ccgccgcggg cgatcctggt gcgctcgggg
     2581 acactgccaa cgcgctcgcc acgggcgaat cgaacggacc gctgctcgcg ccccgggtga
     2641 tcgatccgcg caacgacttc ctgaccgtct cgctgcttca cagcgtgatc gaccacggga
     2701 ccggctacgc ggtaaagcag ctcggacgca gcgacctcgc cggcaagacc ggttccacca
     2761 acgaccacca cgacggctgg ttctgcggat tcaacggcga catggtcgca accgtatggg
     2821 tcggcttcga cgactaccga tcactcggcc ggggcgaatt cggcgccaag accgcgctgc
     2881 cgatctggat ggccttcatg ggacctgcgc tcgaaggcaa gccggaaagc gccctggcca
     2941 tgccgcccgg catcgtcacc gcgaccatcg atcgcagcac cgggctgccc gcgccgcccg
     3001 gcgacaccaa caccatgagc gaaatcttcc gcgtcgaaaa cctcgaccgg ctgcgcgccg
     3061 aggccaaggc gcagcagaac aaggcgccgg cgtacgacat cttttagcga tcatgcctga
     3121 tcgcggctgc ggcggaacgg tcagcgagca caagcggctc gagcaatgga gtccggaacg
     3181 gccttccatg gcccgacttt tcacaacagc tgctccggat aaaactttac ggactccgga
     3241 gctcggcacc tgttccccgt tccggcttcc cgttcccgct tttgcttttc tcccccgctt
     3301 tttgcgacac tgggtccaac gcggcaacag ggggcgattc acgatgcaaa agaccgccga
     3361 gttccaccgg atccacgcgg agggtcgtgc ccaccagttg cggcgccgtg tcgcgatcga
     3421 ggccgcgcgc ctgatcagcg agcagggcat ccgtgattac cggatggcca aacgcaaggc
     3481 cgcgcagcgg atcggcgtgc gcgacgaggg cttcctgccc cgcaaccgcg agatcgagga
     3541 tgcgctgcgc gagcaccagc gcctgtttcg tggcgacgag caagcgcgcg cccagcagtt
     3601 ccggctggaa accgcccggg acgccatgaa gttcctgtcg cgtttcgagc cacgcctggt
     3661 gggctcggtg ctggagggca cttccgacgt ccattcgccg gtcagcctgc acgtgttcga
     3721 cgacacgccg gaggccgtcg cacggttcct ccacgaccac cgcatcgcct tcgagacccg
     3781 cgatcgcacc gtgcgcctcg accggacccg cagcggcgaa ttcccggtcc tgctgttcga
     3841 agccgacggg gtcccggtcg atgtcacgat cttcccgcgc gatgccttgc gccaggcgcc
     3901 gctggaccgc atcgacgagc gcccgctgca gcgggcttcg ctggccgcgg tggagatgat
     3961 gttggcgggc gtcggttgat ccgggacccg ggacgagcgt aaatgctgtc acccccttcc
     4021 atggaagggg tcgcaccaaa ggcgcggggg gatgccgcga agacgcgagc gtcttcccct
     4081 aaaaaaagcc atccccctcg ctcccccttc gcatgcgaag ggggaagaaa cctctatcgt
     4141 ttttcgaccg ccacgtactc gcgcggcgtg gcaccgacgt agacctggcg cggacggccg
     4201 atccggccgc cgggcatctc gtgctgctcg atccagtgcg cgatccagcc ggcggtgcgg
     4261 gcgatcgcga acatcaccgt gaacatttcg gtcgggatcc ggagcgcctt gtagatgatg
     4321 cccgagtaga aatccacgtt cgggtacagt ttgtgctcga tgaagtaatc atccttgagc
     4381 gcggcttctt ccagcttcat cgcgacatcc agcagcgggt cgtggacgcc gagcttgtcg
     4441 agcacttcgt ggcacttcgc gcggatgatc ctggcgcggg ggtcgaagtt cttgtagacg
     4501 cggtggccga agcccatcag gcggaaaccg gactccttgt ccttcgcgcg gtcgatcgcc
     4561 ttgccgacgt tcttctcgtc gccgatttcg gtgagcatct tcagcacggc ctcgttggcg
     4621 ccgccgtgcg ccggccccca cagcgcggcg atgcccgccg ccacggacgc gtacggattg
     4681 gcgccggtcg agccgaccaa ccgcaccgtc gaggtcgagg cgttctgctc gtggtccgcg
     4741 tgcaggatga acaacaggtc cagcgcttcg gcgacgacgg gattgagttc cagcggctcg
     4801 ctcggcacct cgaacatcat gtgcaggaag cggtcgacgt acctcaggct gttgcgcgga
     4861 tagcggatcg gccaaccgat cgaatagcga tacgatgccg cggcgatcgt cggcatcttc
     4921 gccagcaggc ggaccgccgc caggcggcgc tggtccggat cctggatgtc gagggtgtcg
     4981 tggtagaacg ccgacagcga cgcgatcgcg gcgcacaaca ttgccatcgg atgggcgtcg
     5041 tagtggaagc cgtgcaggaa gttcttgagc gactcgtgca tcatcgtgtg gtgcgtgacc
     5101 gagtgctcga acttggtcat ctcagccttg gtcggcaact cgccttccag cagcaggtac
     5161 gcgacttcga ggaagctgga gcgctcggcg agctgctcga tcgggtagcc gcggtacagc
     5221 agcacgccct tctcgccgtc gatgaatgtg atcgcgctct tgcaggaggc ggtcgcggca
     5281 aaccccggat cgaacgtgaa gtggccggtc tcccggtaga gcgatgaaat gtcgacgcag
     5341 ctcggcccct ggctgccctc caccagtggc agggacacgg aatggtcggc ggcggtcagg
     5401 gtgacattct ggttgggcac tggatactcc ttgggtacgt cgcgacgctg ccgcgatcgc
     5461 ggcatggaac cctgcaatta tcgcacggcg ggcgtccgca cgcaggtgcg gcaccggact
     5521 ggcgtcgcaa actcgcggat caggggcacg caaaaacgcc ctccgcgcac tggcgcggaa
     5581 ggcgtcggaa attctgcgca ggatgaagct ggatcccgtg ccatcggcca tccaccatca
     5641 gcctcggcat cctgcttcgg accaatggcc ggccttgggc atcctgccta cggcgctcgc
     5701 cactgcgcga gatgcgttag tcgcatcccg caaacgcagc agctcgccct gcacgggatg
     5761 agttcatccc cggatcaagt ccggggcagg ctctgaactc actttcccgc ggtgtagcgc
     5821 cgcttgaact tgtcgatgcg accgccggtg tccatcacct tctgcttgcc ggtgtagaac
     5881 gggtgcgaag ccgaggaaat gtcgatcttg accagcggat actcctggcc atcctcccac
     5941 tggatggttt cgctgctggt catggtcgag cgcgtcagga acgcgaaatc ggacgcggcg
     6001 tcctggaaga ccaccggctg gtagttcggg tgggtgtcgg ctttcatggg ggtgtccgtc
     6061 tgctgtcgca ggcttgaggc aagggcggaa ttgtaacgtg cccgcgatcc gccgggcaag
     6121 tccggcctca ggcgccggcg aaccgcgcag ccccgccgat ccagcgatcc gcgaggcgcc
     6181 gcgccagcgc cgggtgcgag cgcaacatcg cggcgccgac ccgctgcacc tctggcatga
     6241 gcggggcgtc gcgcacgatg tcggcgatcc ggaacgccat gtccccggtc tggcgggtgc
     6301 cgagcagttc gccgggcccc cgcagccgca ggtcggtttc cgcgatcacg aagccgtcgc
     6361 tggtttcgcg cagcgtttcg agccgctgcc gcgccagcgc cgaaagcggc ggctggtaca
     6421 gcagcacgca gctcgatgcg acgtcgccgc gccccacccg accgcgcaac tggtgcaact
     6481 gggcgaggcc gaggcgctcg gcattctcga tcaccatgag gctggcattg gggacgtcca
     6541 cgccgacttc gaccacggtg gtcgcgacca gcaccgcgca gcggccgtcc ttgaaggcat
     6601 ccatcgccgc ctgtttttcc ctggccttca agcgcccgtg caccaaaccg acttcgactt
     6661 gcggaagcgc cgcggaaagc tgcgcgtgcg cgacctcggc cgcctgcgcc tgcagttgct
     6721 cgccgtcttc gatcaacgtg cacacccagt agacctggcg accggccgcg cacgccgcat
     6781 ggatccgttc gatgacctcc tcgcggcggg tattggcgat cgcgaccgtc cgcaccgggg
     6841 tgcgccccgg cggcaactcg tccaacacgg agacgtcgag gtcggcgtag gcgctcatgg
     6901 ccagcgtgcg cgggatcggc gtcgcggtca gcaccagttg gtgcggcgcg taacctggca
     6961 agaaaccttc cctggtgtgc ttgcgcccgg catcgatgcc tcgcgcttgt ggcgcctgag
     7021 gcgccactac gcccttgtcg cgcagggcca tccgctgctg cacgccgaag cggtgctgct
     7081 cgtccacgat cgcgagcgcc aggccgtgga attccacgcc ctcctgcatc agtgcgtggg
     7141 tgccgacgac caccggcgcg ccggcggcga tctgcgcgag ggccttggtg cgcgcacggc
     7201 cggccacctt gccggccagc cacaccacct cgatcccgag cggctctaac cactcacgga
     7261 agctgcgcag gtgttgctct gcgagcagtt cggtcggcgc catcaaggcc gtctgccggc
     7321 cgtccgcgat cgcgcgcagc gcggccagcg ccgcgacgac cgtcttgccg gagcccacat
     7381 cgccctgcac caaccgcagc atcggcacgc gcttgccgag gtcgcgcccg acttcgtccg
     7441 cgactcgtcg ctgcgcgcgc gtcaggctga acggcaaggc ctcgaggaaa cgcgactcga
     7501 gttccggatc cgccggcagc gcgggggcgc gctgccggcg cacgcgggcg cgccgctgct
     7561 tcaggctcaa gtggtgggcc agcaattcct cgaatgcgag ccgtcgctgc gcgggatgcc
     7621 ggccggcgag cagcgccgca acgtcggcat ccggcggcgg acggtgcacg cccaggagcg
     7681 cgtcgcgcag gggcatcatt ccgagcgacg cgagcatgcc ggcaggcacc agctcgagca
     7741 ccgccgcgtc gggcaggcgc gccagggcct tgtcgatcac cccgcgcagg cgttgctggc
     7801 cgagcccctc cgtcaccggg tataccggcg tcaggttcgt ttcgaccgcc gcctcgccgc
     7861 gcagcacccg gtattgcggg tgcaccattt cgagtccgtg ctggccgtgg cggattgcgc
     7921 cgtagcaacg cagccgcgtg cccgggctga actgttgcgc ctgctggcgc cggaaatgga
     7981 agaacctgag caccagggtg tcgcgcgagg catcgccgat cgcgacgcgc agttgcggcc
     8041 ggtaacggaa gccggattcg accgcttcga ccacgccttc cacctgggcg gattcaccgg
     8101 ccacgaggtc ccggatcgcg gtgatgcggg tgcgatcctc gtagcgcagc ggcaggtgga
     8161 accagagatc ctgcacccgc tcgatcccga gcttgcgcag cgtttgggcg agcgccgggc
     8221 cgacgccggg cagcagcgtc gccggcgcga gtccgggatc gggcgagcgt tcgatgcgcg
     8281 tgtcggcggg ggatgtcacc cgcgcaagat tagcgggcga attcccgcta tgcggaggcg
     8341 ccggtgaccg cgatcacgtc gacctcgacc gccgccccgc gtggcagtgc ggccacgccg
     8401 atggtcgagc gagccgggta cggttcgttg aagtagcgct tcatgacctc gttgaccgcg
     8461 gaaaaattgc cgaggtcggt catgtagatg gcaacgcgaa ccacgtcgtc gaatgtcgca
     8521 ttcgccgcgc ccagcacggc cttcaggttc tcgaacacgc g
//
LOCUS       DASSNH010000195         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1798390, whole genome shotgun sequence.
ACCESSION   DASSNH010000195 DASSNH010000000
VERSION     DASSNH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798390"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1017)
                     /gene="epmA"
                     /locus_tag="VFK00_07225"
     CDS             complement(73..1017)
                     /gene="epmA"
                     /locus_tag="VFK00_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HET7592777.1"
                     /translation="MRAALALRAQFNAAMREFFASRGVMEVETPILSAAGNTEPNIDS
                     FSTGFSGPIAAGARTRYLRTSPEFPLKRLLAAGIGDCYELGRVFRNGEAGASHNPEFT
                     MLEWYRVGFDHRRLALECAQLVRAALALTERDATVHATSYRDWFQSGLGIDPFAADES
                     SLREPLREFSINPDGLTRDDWLDLLVTHCLQPALPHDGITIVYDFPATQCSLARIRHA
                     NPPVAERFELYLGRHELANGYHELNNAAEQRARFASDNLRRRARGQPEPPIDEHLLAI
                     LDALPDCAGVALGVDRLLMHLAATDDIRDVIAFPFAQA"
     gene            complement(1028..>2993)
                     /gene="ligA"
                     /locus_tag="VFK00_07230"
     CDS             complement(1028..>2993)
                     /gene="ligA"
                     /locus_tag="VFK00_07230"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7592778.1"
                     /translation="AAHPELATSDSPSRRVGARPASGFAEVRHAIPMLSLANAFTDSD
                     PPEGVDANHEVRDFVRRVEQGLGTVDPVFSVEPKLDGLAISLIYRDGLFVQGATRGDG
                     TTGEDVTANLHTVKAIPLKLRGRGWPALLEVRGEVFMPKAGFDAYNARMLREGGKPLA
                     NPRNGAAGSLRQLDPHVTAGRPLAFYAYGVYLPDDPDAVIADRHSAAMAKLREWGFPI
                     SPQNELAHGAEGCLAYFRKIGAARATLPYGIDGVVYKVDRYDQQRELGFVSRAPRWAL
                     AHKFPAEEQSTIVEAIDIQIGRTGAATPVARLKPVQVAGVTVTNATLHNADQVARLGV
                     RVGDSVIVRRAGDVIPEVVRVILERRPAHTRPWKMPAHCPICGSAIEREDGEAAARCT
                     GGLVCAAQRREAIRHFASRRAMDIDGLGERYIEDLIAFGYVHSIADLYKLTLDDFVAM
                     KRRAEERDGLVPETVKAGKIATRWAENLIAAIAASKDTTLERFLFALGIPDVGEATAK
                     TLARHFGSLDALMQAGEGALTEVPDVGPIMAAHITAFFAEPRNRDAIGALRAAGVHWP
                     ESTPQRGVAGPLAGQTVVLTGSLSALTRDEAKDKLEALGAKVAGSVSKKTAFVVAGEA
                     AGSKLDKAQALGVEIWDEARLLAFLDEHAA"
BASE COUNT          510 a         1000 c          983 g          500 t
ORIGIN      
        1 atcatttcaa gcgcctcgcg ccgctggtcg actatctgtg cgccgcgcag aacctcgagt
       61 tctgatcgcc tcttacgcct gcgcgaacgg aaacgcgatg acgtcgcgga tatcgtcggt
      121 agcggccaga tgcatcagca agcgatcaac gcccagtgcg acaccggcgc agtcgggcaa
      181 ggcatccagt atcgccagca gatgttcgtc gatcggcggt tcgggttgac cacgcgcgcg
      241 gcggcgcaag ttgtcgctgg cgaagcgcgc gcgctgctcc gctgcgttgt tcaattcgtg
      301 gtagccgttg gcgagctcat gccggccgag gtacagctca aagcgctcgg ccacgggcgg
      361 attggcatgc cggatgcgcg ccaacgaaca ctgcgtcgcc gggaagtcgt aaacgatcgt
      421 gatgccgtcg tgtggcaacg caggctgcaa gcagtgcgtc accagcaaat ccagccagtc
      481 gtcgcgggtc aggccatcgg ggtttatgga aaactcgcgc aggggctcgc gcaacgatga
      541 ttcgtcggcc gcaaacggat cgatcccgag gccggactgg aaccagtcgc gatagctggt
      601 ggcgtggacc gttgcatcgc gctcggtcaa tgccagcgcc gcgcgtacca gctgcgcgca
      661 ttccagcgcg agccggcgat gatcgaatcc gacgcgatac cactcgagca tcgtgaattc
      721 cgggttgtgc gatgcacccg cctcgccgtt gcggaacacg cggcccaatt cgtagcagtc
      781 gccgatgccc gctgccagca gtcgcttcaa cggaaattcg ggcgacgtcc gcaggtaacg
      841 cgtgcgcgcg ccggctgcaa tcgggccgct gaacccggtg ctgaaactgt cgatgttcgg
      901 ctcggtattg ccggccgcgg acaggatggg cgtctcgact tccatgacgc cacggctcgc
      961 gaagaactcg cgcatcgccg cgttgaactg cgcacgcagc gccagtgcag cgcgcaattt
     1021 ggcaccctca ggctgcatgt tcgtccagga acgccagcaa ccgcgcctcg tcccagattt
     1081 caacgccgag cgcctgcgcc ttgtcgagtt tggaaccggc ggcctcgccg gccacgacga
     1141 atgcagtctt cttcgaaacc gagccggcaa ccttggcgcc caaggcctcc agtttgtctt
     1201 tcgcttcgtc gcgggtcagc gcggaaagac tacccgtcaa gaccacggtc tggccggcga
     1261 gcggcccggc gacaccgcgc tgcggcgtgg actccggcca atggacgccg gctgcgcgca
     1321 aggcgccgat ggcatcgcga ttgcgcggtt cggcgaagaa tgccgtgatg tgcgcggcca
     1381 tgatcgggcc gacgtcgggc acttcggtca acgcgccctc gcccgcctgc atcagggcat
     1441 caagggaacc gaaatggcgc gcgagcgttt ttgccgtcgc ctcgccgacg tccgggatgc
     1501 cgagcgcgaa caggaaacgc tcaagcgtgg tgtccttgct cgcggcgatt gcggcgatca
     1561 ggttttcggc ccagcgggtg gcgatcttgc cggccttcac cgtttccggc accagcccgt
     1621 cgcgttcctc ggcacgacgc ttcatcgcga cgaaatcgtc caacgtgagc ttgtacaggt
     1681 ccgcgatgga gtgcacgtaa ccgaacgcga tcaggtcttc gatatagcgc tcgcccaagc
     1741 cgtcgatgtc catcgcgcgg cgtgacgcga agtgccggat ggcctcgcgg cgctgcgccg
     1801 cgcacaccaa cccaccggtg cagcgtgcgg cggcctcgcc gtcctcgcgt tcgatagcgg
     1861 aaccacagat cgggcaatgc gcgggcatct tccacggccg cgtgtgtgcg ggccggcgtt
     1921 cgaggatcac ccgcacgact tcggggatca cgtctcccgc gcggcgcacg atcacgctgt
     1981 cgccgacgcg cacgccgagg cgcgcgacct ggtcggcatt gtgcaaggtg gcgttggtga
     2041 cggtgacccc ggcgacctgc acgggcttca ggcgcgcgac cggggtcgcg gcgccggtgc
     2101 ggccgatctg gatgtcgatc gcttcgacga tcgtcgactg ttcctcggcc ggaaacttgt
     2161 gcgcgagcgc ccagcgcggc gcccgcgaaa cgaatcccag ctcgcgttgc tgatcgtagc
     2221 ggtcgacctt gtaaaccacc ccgtcgatgc cataaggcaa ggtagcgcgc gccgcgccga
     2281 tcttgcggaa gtacgcgagg caaccttcgg cgccgtgcgc cagttcgttc tgcggactga
     2341 tcggaaatcc ccattcgcgc agcttcgcca ttgcagcgct gtggcggtcg gcgatcaccg
     2401 catcaggatc gtccggcaaa tacacgccgt aggcgtagaa cgccaagggc cgtcccgccg
     2461 tcacgtgggg gtcgagctga cgcagcgaac ccgccgcacc attgcgcgga ttcgccagcg
     2521 gtttgccgcc ttcgcgcaac atgcgtgcgt tgtaggcatc gaagccggcc ttgggcatga
     2581 agacttcgcc gcgcacttcc agcaacgccg gccaaccgcg gccacgcaat ttcagcggga
     2641 tggccttgac ggtgtgcagg ttggcggtga cgtcttcgcc cgtagtgcca tcgccgcggg
     2701 tcgcgccctg cacgaacagg ccgtcgcgat agatcaggct gatcgcaaga ccgtcgagtt
     2761 tcggctccac cgagaacact ggatcaaccg tgccgagtcc ctgctcgacc cgccgcacga
     2821 aatcgcgcac ttcgtgattg gcgtccacgc cttcaggcgg gtccgaatcc gtgaacgcgt
     2881 tggcgagcga cagcatcgga atcgcgtggc gaacttccgc aaatcccgac gcgggccgcg
     2941 ccccgacgcg ccgggacgga gaatcggacg tggcaagctc cggatgcgca gct
//
LOCUS       DASSNH010000196         7710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_132812, whole genome shotgun sequence.
ACCESSION   DASSNH010000196 DASSNH010000000
VERSION     DASSNH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132812"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFK00_07235"
     CDS             <1..1185
                     /locus_tag="VFK00_07235"
                     /inference="COORDINATES: protein motif:HMM:NF044995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HET7592779.1"
                     /translation="NGQTLVSGSTALALKAAPDPYDPTRVNVVSQTGATLDGQLQGGN
                     LGGLLDYRSSVLEPARNQLGRVAIAFASAVNAQHQQGMDLDGQLGGDLFSLPAPQAFP
                     AEGNAGNATLTASIADVGALQASDYTLKFDGSNWTVATSGGNPVPVTGSGTAADPLSF
                     DGLQLVVAGSASAGDTFKVQPTRAAAGGIQLAVTDPNQLALAAPFKITGADGNQATAG
                     AMKITDAGNADVLQPVSIEFTSATTYSINGTGSYPYSAGAPIEVNGWSLSLNGTPATG
                     DQFSIQPNSDGVGDNSNALALGNTADVGVLSGGTVSAGVAYASLIADTGTTGAQAQVN
                     LDSQTSLYQQAQQAQQSVAGVNLDEEAGNLIKYQQSYQAAAQVISTANTIFDTLIGAV
                     RG"
     gene            1190..2377
                     /gene="flgL"
                     /locus_tag="VFK00_07240"
     CDS             1190..2377
                     /gene="flgL"
                     /locus_tag="VFK00_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HET7592780.1"
                     /translation="MRISTSWMQQSAINDILDRQSALAVTQAHISSGKRILNPSDDPV
                     AAAQVLNLQHVQAANQQYQRNIDSANTRLGLETSALDQVTSILQRVRTLALQGTNGTE
                     TSSDRGAVATELRQLFSQLVQTANSSDAQGDALFAGNATHTTPFVQGSGFSVSYAGDN
                     GQRMVAAAPGMQVATGDPGGNVFMDIPAGNGRFVVNANPGNTGSVVVGANSVVDTSAY
                     MPGNYTVTFTAPDQWEATDASGTVVGSGSYDGSTSITFNGMQIQLDGQPAAGDTLTVQ
                     SGASQDMFTTIGNLIDSFENMPAGAESSNVVNRQIEGIDQALQGVSGVQASVGARINT
                     LDQQKTSEGDLGVQYQGQISNLQDLDMASAIGQLNLQTVALQAAQQTYTQVQGMSLFD
                     YLR"
     gene            complement(2778..7127)
                     /locus_tag="VFK00_07245"
     CDS             complement(2778..7127)
                     /locus_tag="VFK00_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7592781.1"
                     /translation="MNAILTDRDTATTPALPRLTDFVRGKDGVWQSPSAKPFNYSDGD
                     ATEERIHRIVAAAADKSVYSKELIAQINDWPSEYHFSPRRSNLIRLLDVRPQHRVLEL
                     GCGCGAITKALAETGCRVDAIEGSPRRAGVAAARVREHERARVHHSNFQDVAMEPVHD
                     LVTLIGVLEYSPVYLDADDPFVACLQIAMSALKPGGTLLVAIENRMGLKYFAGISEDH
                     FNQPYYGIEGRYGRKETTTYGRNGLERKLRAAGFGGVSFYFPFPDYKLPEVVIAERAL
                     QHSGLRADDLLQGMEHRDYAGPGKASFNLDMALQSLHEEGLLGDMSNSFLVLATRQGP
                     SPLVAPSLLAQKYTDIRRPGLNVVTSFIEDDRGIEVVKTPIGTSPQPSMAVEHAFTSE
                     RYQSGRNLHTLLKRAAVARRVDEVRALLRRWAGILQEAADADGELPGNWMDAIPSNLI
                     VHEDGSAGLIDREWRMPHPVAASLPFYRGLQLLVGDAAFKPLLPKGDMTAAVRWACEQ
                     AAWTFDADEFKQALDTTNTLWRDVFDNNQWGRADRYRLRRGSTTIQIKPLVLGYADIV
                     AGRKPGEARLLELARTIRPSAGKRIAYAVMAAGSDTAALVRTLDSIAAMGHAAHEVCV
                     IGDIAMPDGHKRIAQVKVATGGMREALNRFLAQSDAEWVQLMAAGDTLSPLFAALLDE
                     KLANHPGLAAVYTDEDSTTDAGPAAPVLKPDVNLDLMRSYPYVGHALAFRREYGLAVG
                     GFAEEPSELGAQDLLFRLLECYGLHTIGHIAEPVLSAGQPYTAWLAMPAVTARSGAVV
                     SAHLDRLRIAHEMQPGGAAGVNRVRYLHDRQPPISIIIPTRDQMPMLNGLIESLLTKT
                     RYKTYELLIVDNDSSDPAACAYLDGIERMQNPQLRVLRWPHPFNYSAINNFAAREARG
                     EYLILLNNDTAILHEDWIEALLNHAQRPEVGIVGAKLHYPDGRIQHGGVVLGLRGPAD
                     HPFIGGAMDAPGYMQRLQVDQNYTAVTAACLMIRKSVYEEVGGLDEVDFKVSYNDVDL
                     CLKVREAGYLTVWTPYARLMHEGSVSQTKVDKTAQEAKGKRFKGEQQAMYRKWLPVLA
                     DDPAYNRNLGLTGAGFDLDQARHLAWQPFEQPLLPRMFCVAADAMGCGQYRIRQPLLA
                     MQREGIAEGLLAPTHLTPVLMERFMPSSIVLQRQLDAKQLAAMQDYRDFSKAFKVYEL
                     DDYLPNLPVKSVHRKDMPKDILRTLRKAVALTDRFAVSTEPLAEQFAGFHADLRVVLN
                     RLPVEWWSGLSSERRKSAKPRVGWAGGAGHRGDLELIVDVVRDLADEVEWVFFGMCPE
                     KLRPYVHEAHEGVAIGGYPAALAALDLDLALAPLEDNTFNACKSNLRLLEYGACGFPV
                     VCSDIVCYRGDLPVTRVRNRYRDWMDAIRMHLADLDATAKAGDALRAAVQRDWMLTGD
                     NLVAWRNAWLPDA"
     gene            7469..>7710
                     /locus_tag="VFK00_07250"
     CDS             7469..>7710
                     /locus_tag="VFK00_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592782.1"
                     /translation="MQFVTVRTHESTRLVLKFAQAVPKQFPRRYTTGCTVPPEQPKPA
                     VRHTVSSGVNPMSQVINTNIMSLTTQNNLNKSQSALA"
BASE COUNT         1373 a         2614 c         2434 g         1289 t
ORIGIN      
        1 aatggccaga ccctggtcag cggctcgacc gcgctcgccc tgaaagccgc gcccgatccc
       61 tacgacccca cccgggtgaa cgtggtgtcg cagaccggcg ccaccctcga tggccagttg
      121 cagggcggca acctgggcgg cttgctcgac tatcgcagca gcgtgctcga acccgcacgc
      181 aaccagttgg ggcgcgtggc gatcgccttc gccagcgcgg tgaatgccca gcaccagcag
      241 ggcatggacc tcgacggcca gctcggcggc gacctgttct cgctgccggc gccgcaagcg
      301 tttccggcgg agggcaatgc cggcaacgcc accctcaccg ccagcatcgc ggacgtgggt
      361 gccctgcagg ccagtgatta cacccttaaa ttcgacggca gcaactggac ggtcgcgacc
      421 agcggcggca accccgtccc ggtcaccgga agcggcaccg ccgccgatcc gctcagtttc
      481 gacgggctgc agctggtggt cgcgggcagc gcgtccgccg gcgacacctt caaggtccaa
      541 cccacgcgcg ccgccgccgg cggcatccag ctcgcggtga ccgatcccaa ccagctcgcg
      601 ttggcggcgc cgttcaagat caccggcgcg gatggcaacc aggccaccgc cggcgcgatg
      661 aagatcaccg acgccggcaa cgccgacgtg ctgcaaccgg tgagcatcga attcaccagc
      721 gccaccacgt attcgatcaa cggcaccggc agctatccgt actccgcggg cgcgccgatc
      781 gaggtcaacg gctggagcct ttcgttgaac ggcacgcctg cgaccggcga tcaattctcg
      841 atccaaccca acagcgacgg cgtgggcgac aacagcaacg ctttggcgct gggcaacacc
      901 gccgacgtcg gcgtgctttc gggcggcacc gtttcagccg gcgtcgccta cgcaagcctg
      961 atcgcggaca ccggcaccac cggcgcgcag gcccaggtca acctggattc gcagaccagc
     1021 ctgtaccagc aggcccagca agcgcagcag tcggtggcgg gcgtcaacct cgacgaagag
     1081 gccggcaacc tcatcaagta tcagcagtcc taccaggccg cggcgcaggt gatctccacg
     1141 gccaacacca tcttcgacac tttgatcggc gcggtaaggg gttgacgcca tgcgtatctc
     1201 gacgagctgg atgcaacaaa gcgccatcaa cgacattctc gatcgtcagt cggcgctggc
     1261 cgtgacccag gcgcacatct ccagcggcaa gcgcatcctg aatccttccg acgatcccgt
     1321 tgcggcggcc caggtgctga acctgcagca tgtgcaggcg gccaaccagc agtaccagcg
     1381 gaacatcgac agcgccaaca cacggctcgg gctggaaaca tccgcgctcg accaggtgac
     1441 ctcgatcctg caacgcgtac gcacgctcgc gctccagggc accaacggca ccgagaccag
     1501 cagcgaccgc ggggcggtcg ccaccgaatt gcgccagttg ttttcgcagc tcgtgcagac
     1561 cgccaattcc agcgacgccc agggcgacgc cttgttcgcc ggcaacgcga cccacaccac
     1621 cccgttcgtg cagggatccg gtttcagcgt cagttatgcg ggcgacaacg gccagcgcat
     1681 ggtggccgcg gcgcctggca tgcaggtggc gacgggcgat cccggcggca acgtcttcat
     1741 ggacatcccg gccggcaacg gacgcttcgt ggtcaatgcg aaccccggca acacgggatc
     1801 ggtcgtggtc ggcgccaaca gcgtggtgga cacctctgcc tacatgcccg gcaattacac
     1861 cgtgaccttc acggcaccgg accagtggga ggccacggac gcgagcggca ccgtcgtcgg
     1921 cagcggcagc tacgacggca gcacctcgat caccttcaat ggcatgcaga tccagcttga
     1981 cggccagcct gctgcgggtg acacgctcac cgtccaatcg ggcgcgagcc aggacatgtt
     2041 cacgacgatc ggcaacctga tcgacagctt cgagaacatg ccggccggcg ccgagtccag
     2101 caacgtcgtc aaccgccaga tcgaaggcat cgaccaggcc ctgcaaggcg tatccggcgt
     2161 ccaggccagc gtcggggcgc gcatcaatac cctggaccag cagaagacct ccgaaggcga
     2221 cctcggcgtc cagtaccagg gccagatttc caacctgcag gacctcgaca tggccagcgc
     2281 catcggccag ttgaacctcc agaccgtggc actccaggcc gcgcagcaga cctacaccca
     2341 ggtccagggc atgtcgctgt tcgattacct gcgctgagcg aaggctcgcg cggcaaagcc
     2401 cattcccctc ccgcttcggg cgcgttcgct tggcttcgcc cttcgcgcga agggcgagac
     2461 aagcggactg cgcgcggatg aaaaacgcag tggcggccaa gacgcgtgcg ctcgcatcgt
     2521 tccgggattc cattgcactt catccgggct acggggcgag atcaatcgcg caggcaaaac
     2581 aggcaggccc gatgaacgcg taactgcgct tgcagcaatg cgatccgagc ggagagtagc
     2641 cggcccgcaa catgagccat atggctgtcc cgaatcagcc ggagcgccgc cgttccgtca
     2701 ctcccggatt ccgttgcacg ccatccgggc ttcgaagctc gacgcccctt caagcggggg
     2761 gcgtcgatcg tttcgcgtca ggcgtccggc agccaggcgt tccgccaggc aaccaggttg
     2821 tcgccggtga gcatccagtc gcgctgcacc gccgcgcgca atgcatcgcc ggctttcgcc
     2881 gtggcgtcga gatcggcgag gtgcatgcgg attgcatcca tccagtcccg gtagcggttg
     2941 cgcacccggg tcacgggcag gtcgccgcga tagcacacga tgtcgctgca gacgaccggg
     3001 aaaccgcagg cgccgtactc gagcaaccgc aggttgctct tgcaggcgtt gaaggtgttg
     3061 tcttcgagtg gcgccagggc caggtcgaga tccagtgcgg ccagggcggc cggatatcct
     3121 ccgatcgcca cgccctcgtg ggcctcgtgc acgtaggggc gcagcttctc cgggcacatc
     3181 ccgaaaaaca cccactcgac ttcgtccgcg aggtcgcgta ccacgtccac gatcaactcg
     3241 aggtcgccgc gatgcccggc gccgccggcc cagccgaccc gcggcttggc ggactttcgc
     3301 cgctcgctcg agagaccact ccaccactcc accggcaggc ggttgagcac gacgcggaga
     3361 tcggcgtgga aacccgcgaa ctgttcggcc agcggttccg tcgacaccgc gaagcggtcg
     3421 gtcagtgcca ccgccttgcg cagcgtccgc aggatgtcct tgggcatgtc cttgcggtgc
     3481 acgctcttca ccggcaggtt gggcaggtaa tcgtccagtt catagacctt gaaagccttg
     3541 ctgaagtcgc ggtagtcctg catggccgca agctgcttcg catcgagctg gcgctgcagc
     3601 acgatgctcg agggcatgaa gcgctccatc agtactggcg tcagatgcgt cggcgcaagc
     3661 aggccttcgg cgatcccctc gcgttgcatc gcgagcaacg gctgccggat acggtactgg
     3721 ccacaaccca tggcatccgc cgccacgcag aacatccgcg gcagcagcgg ctgctcgaac
     3781 ggctgccagg ccaaatggcg ggcttggtcc agatcgaatc cggcaccggt gagcccgagg
     3841 ttcctgttgt aggcgggatc atcggccagc acgggcagcc acttgcggta catcgcctgc
     3901 tgctcgccct tgaaccgctt gcccttggcc tcctgcgccg tcttgtcgac cttggtctgg
     3961 ctgacactgc cctcgtgcat caagcgggca tacggagtcc ataccgtgag atagccggct
     4021 tcacgcacct tcaggcacag gtcgacatca ttgtaggaga ccttgaagtc cacctcgtcc
     4081 aggccgccga cttcctcgta gaccgatttg cggatcatca ggcatgcagc ggtcaccgcg
     4141 gtgtagttct ggtcgacctg caggcgctgc atgtaaccgg gagcgtccat cgccccgcca
     4201 atgaacggat ggtcggcggg cccacgcagg cccagcacca cgcctccgtg ctggatgcgc
     4261 ccgtccgggt aatgcagctt ggcacccacg atccccactt ccggccgctg cgcatgattg
     4321 agcaacgcct cgatccagtc ttcgtgcagg atcgcggtat cgttgttgag caggatcagg
     4381 tactcgccgc gcgcttcgcg ggcggcgaag ttgttgatcg cggaatagtt gaacggatgc
     4441 ggccagcgca agacgcgcaa ttgcggattc tgcatccgct cgatgccgtc caggtaggcg
     4501 caggcggccg gatcactgct gtcgttgtcg acgatcaaca gctcataggt cttgtagcgg
     4561 gtcttggtca gcaagctttc gatcagcccg ttcaacatgg gcatctggtc gcgggtcggg
     4621 atgatgatcg aaatgggcgg ttgtcggtcg tgcaggtagc gcacccggtt cacccccgcc
     4681 gcgccacccg gctgcatttc gtgcgcaatg cgcaggcgat cgaggtgcgc ggaaaccacc
     4741 gcgccagaac gcgcagtcac cgcaggcatc gccagccacg cggtgtacgg ttgcccggcc
     4801 gaaagcaccg gctcggcgat gtggccgatg gtgtgcaggc cgtagcattc gagcagccgg
     4861 aacagcaggt cttgtgcgcc cagttccgaa ggctcctcgg cgaatccccc cacggccagg
     4921 ccatactcgc gccggaatgc cagcgcgtgg cccacgtacg ggtagctacg catcaggtcc
     4981 aggttgacgt cgggcttcaa caccggggcg gcgggtcccg cgtcggtggt gctgtcttcg
     5041 tccgtgtaaa cggccgcgag cccgggatgg tttgcgagct tttcgtcgag cagcgcggca
     5101 aacaatggcg acagggtgtc gccggcggcc atgagctgca cccattcggc atcgctctgc
     5161 gcgaggaatc ggttgagtgc ctcgcgcatc ccgcccgtgg cgaccttgac ttgtgcgatg
     5221 cgcttgtgcc cgtccggcat ggcaatgtcg ccgatgacgc aaacctcgtg cgcggcatgg
     5281 cccattgcgg cgatgctgtc cagcgtgcgt accaaggcag ccgtatcact gcccgcggcc
     5341 atcacggcat aagcgatgcg cttgcccgcg gatggacgaa tcgtcctggc cagctccagc
     5401 agccgcgcct cacccggctt gcgccccgcg acgatgtcgg catagcccag caccaggggc
     5461 ttgatctgga tcgtcgtgct gcctcgccgc aggcgatatc gatctgcccg accccattgg
     5521 ttgttgtcga acacatcgcg ccaaagggtg ttggtggtat cgagtgcctg cttgaattca
     5581 tccgcgtcga acgtccaggc ggcttgctcg caagcccagc gcaccgcggc ggtcatgtcg
     5641 cctttgggca acaacggctt gaacgcggcg tcgcccacca gcaactgcaa gccacgatag
     5701 aacggcagcg atgcagccac cggatgcggc attcgccatt cgcgatcgat caagccggcg
     5761 ctgccgtctt cgtgcacgat caggttggac ggaatcgcgt ccatccagtt gcccgggagt
     5821 tcgccatcgg catccgcggc ttcctggaga atgccggccc accggcgcag cagcgcgcgc
     5881 acctcgtcga cccgccgcgc gacggcggcc cgcttcaaca acgtgtgcag attgcgcccg
     5941 gattgatagc gttcgctggt gaaggcgtgc tcgacggcca tgctcggctg gggcgatgtg
     6001 ccgatgggcg tcttgacgac ctcgatgccc ctgtcgtctt cgatgaagct cgtgacgaca
     6061 ttgaggccgg ggcgccggat atccgtgtac ttctgtgcca gcaacgatgg cgccaccagc
     6121 ggtgaaggcc cctgccgggt cgccagcacc aggaacgaat tggacatgtc acccagcagg
     6181 ccttcctcat ggaggctttg cagggccatg tcgagattga agctcgcctt gcccgggccg
     6241 gcgtagtcgc ggtgctccat gccctgcagg aggtcgtccg cacgcaaccc cgagtgttgg
     6301 agcgcacgtt ccgcaatcac cacttccggg agtttgtagt cggggaacgg gaagtagaac
     6361 gacacgccac caaaacctgc cgcgcgcagt ttgcgttcca acccgttgcg cccgtaggtg
     6421 gtggtttcct tcctgccgta gcgtccttcg atgccgtagt agggctggtt gaagtggtct
     6481 tcggagatgc cggcgaagta cttcaggccc atcctgtttt cgatggccac cagcaaggtg
     6541 ccgccgggct tgagcgcgct catggcgatc tggagacacg ccacgaaggg atcgtcggcg
     6601 tccaggtaga ccggcgaata ttccagcacc ccgatcaagg taaccaggtc gtgtacgggc
     6661 tccatcgcca cgtcctggaa gttggagtgg tgtacccgcg cgcgttcatg ctcacggacc
     6721 cgtgcggccg ccacgcccgc gcgccgtggc gagccctcga ttgcatccac gcggcagccg
     6781 gtttccgcca gtgccttggt gatcgcgccg caaccgcatc ccagttcgag aaccctgtgt
     6841 tgcgggcgga catccagcaa gcggatcagg ttggatcgac gcggcgagaa atgatattcg
     6901 ctgggccaat cgttgatctg ggcgatcaac tccttcgaat agacgctctt gtcggcggcc
     6961 gcggccacta tgcgatggat gcgttcctcg gtcgcatcgc cgtcggaata attgaagggc
     7021 ttggcagagg gcgactgcca caccccgtct tttccgcgta cgaagtcggt gaggcgaggc
     7081 agcgcgggcg tggtggcagt atctcgatcg gtcaggatgg cattcatgga ttccggggat
     7141 cctcccgcgg ggtggcggct gtttggatgg cgacaccggc ccaggggctg tccggcgtcg
     7201 gttttctgac gcgcgcacac gtcatgccct gtaggcagca agatcggtgc cagctctccc
     7261 aaggcccgtt cgggcggctc cccggcgccc ggcccattga gtctccacgc gttgacgggc
     7321 ctgcggcagc cagcgcagtc gtcgccctgg cgttccgcgg accagccctg cccggccccg
     7381 gaaaccaggg ggttcggcgg gcaagtcctc ccttcgccgg ccgcaccgta atcgcagcca
     7441 tccacaccaa accggccggc ctgcgacgtt gcaattcgtg accgtgcgca cacacgaaag
     7501 cacgcggctt gttctcaagt tcgcgcaagc cgtgccgaaa caattcccga ggcggtacac
     7561 aaccggatgc acggtaccgc cggaacaacc caagccggct gtccggcata ccgtatcttc
     7621 aggagtaaat cccatgtctc aggtcatcaa caccaacatc atgtcgctga ccacgcagaa
     7681 caatctgaac aagtctcagt ctgcgttggc
//
LOCUS       DASSNH010000197         6107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1014794, whole genome shotgun sequence.
ACCESSION   DASSNH010000197 DASSNH010000000
VERSION     DASSNH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014794"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFK00_07255"
     CDS             <1..491
                     /locus_tag="VFK00_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HET7592783.1"
                     /translation="GVAEGRRLLDTLGWAALLPLTLAALGGVFAATGVGTAVADLVRM
                     VIPVDSALACVVAYGLGMVLFTVIMGNAFAAFPVLTAGVGLPLLIQLHGANPAILGSI
                     GMVTGYCGTLFTPMAANFNIVPVALLELPNQNDVIRAQWKTGAALLVVNLALMYVLVF
                     RF"
     gene            488..1153
                     /gene="pcp"
                     /locus_tag="VFK00_07260"
     CDS             488..1153
                     /gene="pcp"
                     /locus_tag="VFK00_07260"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET7592784.1"
                     /translation="MKPATRHHEPAILLTGFTPFGGEAVNPSWQAVRELDGSVIQGHR
                     VTAVELPTEFDGSLPALWHALRATEPRVAIAVGLAGGREGISLERVAINLVDARIPDN
                     AGAQPIDVPVLRSGAAAFFSTLPLKASLLALQDAGIAAHISQTAGTYVCNQVFYALMH
                     ALRRSRNVRAGFVHVPWLPEQAERHHAFGMPLEDIQRAIGIIIHTALATTVDAKVSGG
                     AES"
     gene            complement(1212..1619)
                     /locus_tag="VFK00_07265"
     CDS             complement(1212..1619)
                     /locus_tag="VFK00_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592785.1"
                     /translation="MNLRPFLARLGGAALLATMLALAACNGNVRSGGESVVNVPHQYD
                     MTIGAFKDNQFLLDGAVLTAPDLEAHFRYLQDQKQLPKTVLLKDSDSDKISNSDLEEF
                     ASLQLSFHFTGYVEHKGKLTLMSAVEAPKPKQK"
     gene            complement(1873..2436)
                     /gene="dcd"
                     /locus_tag="VFK00_07270"
     CDS             complement(1873..2436)
                     /gene="dcd"
                     /locus_tag="VFK00_07270"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET7592786.1"
                     /translation="MSIKSDNWIRRMADNGMIDPFEPGQVKENGGGRIVSYGTSSYGY
                     DVRCADEFKIFTNINSTIVDPKAFDSRNFIDFKGDCCIIPPNSFALARTVEYFRIPRN
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYAHEGVAQMLFLE
                     SDEVCTTSYKDRGGKYQGQKGVTLPKT"
     gene            complement(2561..3655)
                     /gene="apbC"
                     /locus_tag="VFK00_07275"
     CDS             complement(2561..3655)
                     /gene="apbC"
                     /locus_tag="VFK00_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HET7592787.1"
                     /translation="MASAVTEAQVRARLAGIIDPHTGASLEESGEVHAVGVDGDRVAV
                     EIALGYPADSWRNQFAGEIRELLEADAAIAQATVSVTTRIATHKVQGNLMPLPGVKNI
                     IAVASGKGGVGKSTVAVNLALALVAEGANAGVLDADIYGPSQPLMLGIHGRPESPDGK
                     SIIPMTNHGLQVMSIGFLVGEDTPMVWRGPMVTQALSQLLHSTRWKDLDYLVVDMPPG
                     TGDIQLTLTQRVPVAGAVIVTTPQDIALLDARKGLKAFEKVQVPVLGVIENMATHVCS
                     NCGHEEHVFGEGGGARIAEQYGVPLLGSLPLDVRIREQADGGNPTVSAMPDSDLAMRY
                     REIARNTAARLSLQSERDMTGVPNIRMADD"
     gene            3803..4255
                     /locus_tag="VFK00_07280"
     CDS             3803..4255
                     /locus_tag="VFK00_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HET7592788.1"
                     /translation="MRTAVRSMLITAALLLATAAYAATDTATPVGTWTQIDDDTGKPK
                     SIIEITEQSDGTLQGVVKQVLFSDQGPHPICDKCEGERHNQPVEGMTIMWGVKKDGDV
                     WDGGQILDPAKGKSYRVELSLRDDGQKLDVKGFLLFFHRTQTWIRKTD"
     gene            4528..>6107
                     /locus_tag="VFK00_07285"
     CDS             4528..>6107
                     /locus_tag="VFK00_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET7592789.1"
                     /translation="MATRFALIPQTCAGALLAALLGISPGAQAQTTAAPATPSTTSTA
                     PRSGDYARLEAEARNVTITRDDWGIPHIHGKTDADAVFGMIYAQCEDDFSRVQSNLLD
                     ALGWTARAEGKSAIWSDLREQLWIDPAVLKQDYAKSPGWLKKLMNAWADGLNWYLANH
                     PEVQPVIAHYEPWMALSFTEGSIGGDVTRVELAPLAKFYGADPATTLALADTTPPTWV
                     DPIGSNGIAIAPKLSADGHALLLINPHVTFNFRTESQMSSDEGLDAYGASTWGQFFIY
                     QGFNQHVGWMHTSTGIDAVDEFAETIEQKNGKYFYRFGKQLLPVKTRDITIRYRDDDG
                     SMAERTFTAYFTRHGPIVRSENGKWIAEALMNRPVPALEQSWLRTKATDLASYMKVAE
                     LKANSSNNTLFADDKGEIAFLAPQFIPKRDNSFDYLEPVDGSNPATAWEGMTPLKDMP
                     NSIDPPNGWVFNTNNWPYSAAGQYSPKRTDFPQYMDSFGENARGIHATLMLTGAKDFT
                     KAKVITDAFDSYLPAFAR"
BASE COUNT         1069 a         2053 c         1939 g         1046 t
ORIGIN      
        1 cgggcgtcgc cgaaggacgg cgcctgctcg acacgctggg ctgggcggcg ctgctgccgc
       61 tgaccctggc ggcgctcggc ggcgtgttcg ccgcgaccgg cgtcggcacc gcggtcgccg
      121 acctggtacg catggtcatt cccgtcgaca gcgccctcgc ctgcgtggtc gcctacggcc
      181 tcgggatggt gctgttcacg gtgatcatgg gcaatgcctt cgccgcattc ccggtactga
      241 ccgccggcgt cgggctgccg ctgctgatcc agttgcacgg ggcgaatccg gcgatcctgg
      301 gatcgatcgg catggtcacc ggctactgcg gcacactgtt cacgccgatg gccgcgaact
      361 tcaacatcgt gccggttgcg ctgctcgaac tgccgaacca gaacgacgtg atccgcgccc
      421 agtggaaaac cggcgcggcg ctgctggtcg tgaacctcgc gctgatgtac gtcctggtat
      481 tccggttttg aagccggcca cccgccacca cgagccggcc atcctgctca ccggcttcac
      541 gccgttcggg ggcgaggccg tcaatccgtc gtggcaggcg gtacgcgagc tggatggcag
      601 cgtcatccag ggacatcgcg tcacggcggt cgaactgccc accgaattcg acggctctct
      661 gccggccctg tggcacgccc tgcgggcgac cgaaccccgc gtcgcgatcg cggtcgggct
      721 cgccgggggg cgcgagggca tttcgctcga gcgggtggcg atcaacctcg tcgatgcgag
      781 gattccggac aatgcgggcg cgcagcccat cgacgtgccg gtgctgcgca gcggcgccgc
      841 ggcgttcttt tccacgctgc cgctcaaggc atcgcttctg gcattgcagg acgcaggtat
      901 cgccgcgcac atctcgcaga cggcgggcac ctacgtctgc aaccaggtgt tctacgcgct
      961 gatgcacgcc ctgcgccgca gccgcaacgt ccgggcgggt ttcgtgcacg tgccctggct
     1021 cccggagcag gcggagcgcc accacgcatt cggcatgccg ctggaggaca tccagcgcgc
     1081 catcggaatc atcatccaca ccgcgcttgc gacgaccgtc gacgcaaagg tcagcggcgg
     1141 cgccgaaagc taacggagct cgcgcggctg cgagcccgcc cacctgcagg ctcggcggac
     1201 atgggaagcc gctatttctg tttcggcttg ggcgcctcga cggcgctcat cagcgtgagc
     1261 ttgcccttgt gctccacgta gcccgtgaag tggaagctca actgcaggct ggcgaattcc
     1321 tcgaggtcgc tgttgctgat cttgtccgaa tcggaatcct taagcagcac cgtcttcggc
     1381 aactgcttct ggtcctggag gtagcggaag tgtgcttcga ggtcgggcgc cgtgaggacc
     1441 gcgccgtcga gcaggaactg gttgtccttg aacgcgccga tcgtcatgtc gtactggtgc
     1501 ggcacgttga ccacgctctc gccaccgctg cggacattcc cgttgcacgc ggccagcgcg
     1561 agcatcgttg ccaacagcgc cgcgccgccg aggcgcgcaa ggaacggacg caggttcatg
     1621 ggacggtctc cgtgggtcga tgaaagcagg cggcagcgct gcagccgtgc aagtctgcca
     1681 caaacgagct gaatccgcgc gccgccatcc gttgcgggca aatggtgcgg ctaaaaaagt
     1741 catttgacgc tgatcaacgc ggatagaagc tgatttcctg cgctttaccc gcgctcatcc
     1801 gcgcaaatcc gtgtcggaaa gtttgtattc cgacatgtca gcgcgagcca caggcgaaag
     1861 cgagcagagg cttcaggtct ttggcaacgt cacgcccttc tgcccctggt acttgccgcc
     1921 acggtccttg taactggtcg tgcacacttc gtcggattcc aggaacagca tctgcgccac
     1981 gccctcgtgg gcatagatct tggcgggcaa cggggtggtg ttggagaact ccagcgttac
     2041 gtggccctcc cattccggtt ccagcggcgt cacgttgacg atgatgccgc agcgcgcgta
     2101 ggtgctcttg ccgaggcata tcgtcagcac gttgcgcggg atccggaagt attcgaccgt
     2161 gcgggcgagc gcgaacgaat tgggcgggat gatgcagcag tcgcccttga agtcgatgaa
     2221 gttgcgcgaa tcgaaggcct tgggatccac gatggtcgaa ttgatgttgg tgaagatctt
     2281 gaactcgtcg gcgcagcgta cgtcgtagcc gtagctcgag gtgccgtagg agacgatgcg
     2341 gccgccgccg ttttccttga cctggccggg ctcgaatggg tcgatcatcc cgttgtcggc
     2401 catccggcgg atccagttgt cgctcttgat gctcatgggg aacggtgagg gtttagggac
     2461 ttgggattag ggactggact gcagaccggg gactgcagac cggggaccag ggaccggaaa
     2521 tgctagcagg ccatctctcg cccctcgccc ctgcttcttc tcagtcatcg gccatgcgga
     2581 tgttcggcac gccggtcatg tcgcgctcgc tttgcagcga caggcgcgcc gcggtgttgc
     2641 gcgcgatttc gcgataacgc atggcgagat ccgaatctgg catcgcggac accgtggggt
     2701 tgccgccgtc ggcctgttcg cggatccgca cgtcgagcgg cagcgagccg agcaacggca
     2761 ccccgtattg ctccgcgatc cgcgcgccgc caccttcacc gaacacgtgt tcctcgtggc
     2821 cgcagttcga acagacatgg gtggccatgt tctcgatcac cccgagtacc ggcacctgca
     2881 ccttctcgaa ggccttgagg cccttgcggg catccagcag cgcgatgtcc tggggcgtgg
     2941 tcacgatcac cgcacccgcc accggcacgc gctgggtcag cgtcaactgg atatcgcccg
     3001 tgccgggcgg catgtccacg acgaggtagt cgaggtcctt ccagcgggtg gaatgcagca
     3061 actggctcag cgcctgggtc accatcggcc cgcgccagac catcggcgtg tcttcgccga
     3121 ccaggaaccc gatcgacatc acctggaggc cgtggttcgt catcggaatt atcgacttgc
     3181 cgtccggcga ttccgggcgg ccgtgtatgc cgagcatcag cggctggctg ggtccgtaga
     3241 tgtcggcgtc gaggacacct gcgttggcgc cttcggcaac cagcgccagc gcgaggttca
     3301 ccgcgaccgt ggacttgccg acgccgccct tgccggatgc gacggcaatg atgttcttga
     3361 cgcccggcag cggcatcagg ttgccctgca ccttgtgggt cgcgatgcgc gtcgtcaccg
     3421 agaccgtcgc ctgcgcgatc gccgcgtcgg cttcgagcag ctcccggatt tcgccggcaa
     3481 actgattgcg ccaactatcg gccgggtaac cgagtgcaat ttcgaccgcg acccggtcgc
     3541 cgtccacgcc gaccgcatgc acctcgccgc tctcctcgag cgaggcgccg gtgtgcggat
     3601 cgatgatgcc tgcgaggcgg gcgcggacct gcgcctcggt gacagcggat gccatgcaac
     3661 ttccctgcga agaccagcct ttcattatgc cagagggtgc cgcggcgacc ctgattccgg
     3721 tcagatccgg cgccggcgcc ggggcagttg acgcgcacgc gggcttcgcg caggctagcc
     3781 ccaaccaccc cggagagttt ccatgcgcac cgccgtccgc tccatgctga tcaccgccgc
     3841 gctgctgctc gccaccgccg cgtacgccgc caccgacacc gccacgccgg tcgggacgtg
     3901 gacccagatc gacgacgaca ccggcaagcc caagtccatc atcgagatca ccgagcaatc
     3961 cgacggcacg ctgcagggcg tcgtcaagca ggtgctcttt tccgaccagg gcccccatcc
     4021 catctgcgac aagtgcgagg gcgagcgcca caaccagccg gtggaaggca tgaccatcat
     4081 gtggggcgtc aagaaggatg gcgacgtgtg ggatggcggc cagatcctcg atcccgccaa
     4141 gggcaagagc taccgcgtcg aactgtcgct gcgcgacgac ggacagaagc tcgacgtcaa
     4201 gggcttcctg ctgttcttcc atcgcaccca gacgtggatc cgcaagacgg attgagccac
     4261 gcggtacctg acgaagagcc atccccctcg atcccccttc ctgcggaagg gggaggacaa
     4321 gcgcgcaatc ggctgcctca cccccttcca actgaaaggg gcgatgcgca gcatcggggg
     4381 atgcttggaa aagccttgcg caaccaggga atacgggaat cgctccgccc gttcgtccaa
     4441 tctcatgcgc cgccgcccgc gtcgacccac gataagcggt ttcacgctaa ggttgcgggg
     4501 ttccagcact cgtcgcgggg aagatcgatg gccacacgtt ttgcgttgat tccacagacc
     4561 tgcgccgggg cgctgctcgc ggcgctcctg gggatttccc ctggcgcgca ggcgcagacg
     4621 acagccgcgc cggcaacgcc atcgacgacg tcgactgcac cccggagcgg cgactacgcg
     4681 cgtctcgagg ccgaagcgcg gaacgtcacg atcacccgcg acgactgggg catcccgcac
     4741 atccacggca agaccgatgc ggatgcggtg ttcgggatga tctacgcgca atgcgaggac
     4801 gacttcagcc gcgtccagag caacctgctg gacgcgctcg gctggaccgc gcgggccgaa
     4861 ggcaagtccg cgatctggtc cgacctccgc gaacagttgt ggatcgaccc ggcggtgctg
     4921 aagcaggact acgcgaaaag tcccggctgg ctcaagaagc tgatgaacgc gtgggcggac
     4981 gggctcaact ggtacctcgc caaccacccc gaggtgcaac ccgtcatcgc gcactacgaa
     5041 ccgtggatgg cgctgtcctt caccgaaggt agcatcggtg gcgacgttac ccgcgtggag
     5101 ctcgccccgc tcgcgaagtt ctacggcgcc gatcccgcga ccacgctcgc gctggccgac
     5161 acgacgccgc cgacctgggt cgatccgatc ggctccaacg gcatcgcgat cgcgccgaag
     5221 ctgtccgccg acggccacgc gctgctgctg atcaatccgc acgtcacctt caatttccgc
     5281 accgaatcgc agatgtccag cgacgagggc ctcgacgcct acggcgcctc gacctggggc
     5341 cagttcttca tctaccaggg cttcaaccag cacgtcggct ggatgcatac ctcgaccggc
     5401 atcgatgccg tggacgagtt cgccgaaacc atcgaacaga agaatggcaa gtatttctac
     5461 cggttcggca agcaactgct gccggtcaag acccgcgaca tcacgatccg ctatcgcgac
     5521 gatgacggct cgatggccga gcgcaccttc actgcgtact tcacccggca cggcccgatc
     5581 gtccgcagcg agaacggcaa gtggatcgcg gaagccctga tgaaccgacc cgttccggcg
     5641 ctggagcaga gctggctgcg caccaaggcg acggaccttg cgagctacat gaaggtggcc
     5701 gaactcaagg ccaattcctc caacaacacc ctgttcgccg acgacaaagg cgagatcgcg
     5761 ttcctcgccc cgcagttcat tcccaagcgc gacaacagtt tcgattacct cgaacctgtc
     5821 gacggcagca atccggccac cgcctgggag ggcatgacgc cgctcaagga catgccgaac
     5881 agcatcgatc cacccaacgg ctgggtgttc aacaccaaca actggcccta ctccgccgcc
     5941 ggccagtaca gcccgaagcg cacggacttt ccgcaataca tggattcgtt cggcgagaac
     6001 gcgcgtggca tccacgcgac cctgatgctc acgggcgcga aggatttcac caaggcgaaa
     6061 gtcattaccg acgcgttcga ttcctacctg cccgcgttcg cccggtt
//
LOCUS       DASSNH010000198         6852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1774108, whole genome shotgun sequence.
ACCESSION   DASSNH010000198 DASSNH010000000
VERSION     DASSNH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774108"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFK00_07290"
     CDS             <1..635
                     /locus_tag="VFK00_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592790.1"
                     /translation="RSAVANNEAFRSALANNEAFRAVLANNEAFRSAVTDNEAFRSVL
                     ANNAAFRSALADNEAFRSALANNDALRSALAHSAAFRSAMANNAAFRSAVANSAAFRS
                     ALANNDAFRSAVASNAAFRSALADNAAFRSALTHNEAFRSALANNEAFRSVLANNAAF
                     RQALRSEAFRQALQSAAFRNAMQSEAFRQALRSDAFRQAMATDAERSAQR"
     gene            814..3759
                     /locus_tag="VFK00_07295"
     CDS             814..3759
                     /locus_tag="VFK00_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592791.1"
                     /translation="MKRWGLVALLCLAAALPGRAIAKSPPFELDMLETRDLRVLYTPG
                     DAYLAPYAVQTFENSMDFQRRAFDYTPWERTTVVLTDFSDYGDAGTSVSPHDQVTVYI
                     APDDLTLETSPGSERMFMTANHELVHVATMDGWNQRDERWRDFFNGKPRATDEHPETI
                     LYNYLTVPRSNVPRWYLEGIAVFMETWMSGGIGRAQGAYDEMVFRAMVRDHAHFYSPL
                     GLVSKGTAVDFQTMTNAYLYGTRFISYLALHYSPQMVIDWYRRGKDSKAYYADQFEQV
                     FHVPLDRAWNDWIAWEHSFQGKNLEKVREFPLTKARRLTGRALGNVSQLYFDAQNDSL
                     IGAFQYPGVLAYVGVLSLKDGSIRHLADIKGPMAYRVASTAYDPASKTFFYTADNQEL
                     RDLMEVDVRNGRSRMLLKDARIGDLAFDTADRSLWGLRHLNGYVTLVRIPYPYKDWTQ
                     VHTWPYGDDPFSLAVSKDGSMLSMSVATIDGHKMLRIFKTADLLAGTVEPFAHFDFGQ
                     ATPEGFVFTADGRYLYGSSYYTGISNVYRYDIATKKIDAVSNAETGFFRPVPLADGKL
                     VALEYTGQGFVPVMIDPAPLQDLSAITFLGTRIADQHPVVKAWGVGSPDKVPLKSTIT
                     GKGKYRPSHQIRPQTRYPVVEGYRGGVALGWHFDFTDALNLYKLGVTAAVSRGGQGGA
                     GERLHLTVDYQGMNWYAHYWHNHADFYDLFGPVETSLKGDALMGGYKRALVFDSPRQM
                     DLDVGAAYYTGLATLPGSQNVAAQVNDIFAAHTSLMYKNRRASLGGLDREKGLSWNVD
                     ATAYVAGGKTVPQLRGGFQFGFLLPLPHTSLWSYTSAGVSGGQRSSALANFYFGGFHN
                     NYVDNQDPKQYRGYDSFPGFEIDEISGHTFVKQMVELNLTPLRFAEVGTPGFYLKWLR
                     PALFAGTLVTDPNHAAYRHTVRDLGVQIDIDISVLNHLPMTLSAGYARGFNGPDGDSD
                     EWMLSLKIL"
     gene            3761..4789
                     /locus_tag="VFK00_07300"
     CDS             3761..4789
                     /locus_tag="VFK00_07300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET7592792.1"
                     /translation="MHGAPSILDLLSIIPVGLLPVITFLLLLVYLDSYQMVRMRLVVA
                     VLAAGGVVAIVCYALNGGLMTLLGMNLTHYSRWVAPVVEETLKASVVVWLFRTHRVGF
                     LVDSAILGFAVGAGFAVVENFYYLYLVNDTTLTVWIVRGFGTAMMHGGVVAIFAVLSQ
                     TLAERSVAVRPIHCLPGLAVAISIHSIFNHFYVSPVLSTLGVMVILPPVLYLVFRISA
                     YRTHRWLEVDFDEDAELMRMIDSGHFDRTAAGIFLSGLRDKLDGTLVADMFCYLRVYT
                     ELALRAKGLLLARENGLDVEMDRETREKFAELRYLEHSIGVAGRLTMRPFLQMTTKDL
                     WQLYVLGK"
     gene            complement(4771..6054)
                     /locus_tag="VFK00_07305"
     CDS             complement(4771..6054)
                     /locus_tag="VFK00_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET7592793.1"
                     /translation="MAAADLSADPGLLEDVCAAIRTLDGSGSAPASVALVEVARTRTG
                     ACAAALWLRDRPSGELSMQVPAREPLPRLQPGVGLAGRCAQSAQMLRLDNLSGNPELV
                     DIRLLDSANAPSVGAALNVPLRAGREVLGVLQLLHASPCGFGAEAVLVAETVAAQLAL
                     VLRGAAHDAAMGAASALHTEVEVAREIQRGTLPTEMPSVPGYDLHGHFQPATYAGGDM
                     LDVVKFSQGVFLLLGDATGHGFGPALSAMQMQGMLRVAFRLGADLDQAYRNVNNQLTE
                     DLSDDRFITAFMGLLDAGAHRVRYQCAGQGPLLHYHAADDRCEWLQPNAFPVGVMELE
                     EVPASASLELAPGDILALISDGLYERLDAGNAEFGPGRVAEVVRSGQHLSMAVLCRRL
                     LAAADDFAAGVAAADDVTLILVRRLPADAAHLPST"
     gene            complement(6232..>6852)
                     /gene="fghA"
                     /locus_tag="VFK00_07310"
     CDS             complement(6232..>6852)
                     /gene="fghA"
                     /locus_tag="VFK00_07310"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HET7592794.1"
                     /translation="MLVAPDTSPRDTGIPGATDDWQFGEGASFYVDATQAPYSSRFRM
                     ETYVVEELPKVIAGRFPADFARCGIMGHSMGGHGALTLALRHPGRYRSLSAMSPIAAP
                     TRVPWGDRKIFILYLGSDREEWKRHDACELLRAGASFPGTILIDQGEEDPYLAEQLRP
                     ELFEQACRETGQPLTLRMQPGYDHSYWFVQSFIEDHLRHHARSLCA"
BASE COUNT         1178 a         2256 c         2205 g         1213 t
ORIGIN      
        1 tccgctcggc cgtggccaac aacgaggcgt tccgatcggc gttggctaac aacgaggcgt
       61 tccgtgcagt tctcgccaat aacgaggcat tccgctcggc cgtgacagat aacgaggcct
      121 tccgctcggt gcttgccaac aacgcggcgt tccgttcggc cctggccgac aacgaggcat
      181 tccgatcggc gcttgccaac aacgacgcgc tgcgttcggc gctcgcccac agtgccgcgt
      241 tccgctcggc catggccaac aatgctgcgt tccgctcggc cgtggccaac agcgctgcgt
      301 tccgctcggc gctggccaac aacgacgcgt tccgctcggc cgtggccagc aatgcggcat
      361 tccgctcggc gcttgccgac aacgcggcgt tccgttcggc cctgacccac aacgaagcgt
      421 tccggtcggc gctcgccaac aacgaggcgt tccggtcggt actcgccaac aacgcggcct
      481 tccgccaggc gctccgcagt gaagcgttcc gccaggcgct gcaaagtgcc gccttccgca
      541 atgccatgca aagcgaggca tttcgccagg ccctgcggag cgacgcattc cgccaggcga
      601 tggccaccga tgccgaacgg agcgcgcagc gctaaacatg gcccggggcg cccgacctga
      661 ggggggcgct ccaggcgggc ccgtcgttcc ggaaagccgc ccgcgattgc gggcggcttt
      721 gtttgcggag gggaatattg ccgccggcca ttgttgatgc aagcgggcca acgagcccat
      781 acttgcgccg gggttgatca acgggggtga tcgatgaagc gctggggatt ggtcgcgttg
      841 ttgtgtctcg ccgcggcgct gccgggccgc gcaatcgcca aatccccgcc cttcgagttg
      901 gacatgctcg agaccaggga cctgcgggtg ctgtacacgc cgggcgatgc ctatctcgcc
      961 ccgtacgcgg tccagacctt cgagaattcg atggatttcc agcgccgcgc tttcgattac
     1021 acgccgtggg agcgcaccac ggtcgtgctc accgacttca gtgattacgg cgacgcgggc
     1081 acttcggtct ccccgcacga ccaggtgacg gtgtacatcg cgcccgacga cctgaccctg
     1141 gagacctcgc ccggcagcga acgcatgttc atgacggcca accacgagtt ggtgcacgtg
     1201 gcgacgatgg acggctggaa ccagcgggac gagcgctggc gggacttctt caacggcaag
     1261 ccgcgcgcca ccgacgagca tcccgaaacc atcctctaca actacctgac ggtgccgcgg
     1321 tcgaacgtgc cgcgttggta cctggaaggc attgcggtgt tcatggaaac ctggatgtcg
     1381 ggcgggatcg gacgcgcgca gggcgcctac gacgaaatgg tgtttcgcgc catggtgcgc
     1441 gatcacgccc atttctacag cccgctcggg ctggtctcga aaggcaccgc ggtcgacttc
     1501 cagaccatga ccaatgccta cctgtacggc acccgtttca tcagctacct ggccttgcat
     1561 tactcgccgc agatggtgat cgactggtac cgtcgcggca aggacagcaa ggcgtactac
     1621 gccgaccagt tcgagcaggt gttccacgtg ccactggaca gggcgtggaa cgactggatc
     1681 gcatgggagc attcgttcca ggggaagaat ctcgaaaagg tgcgggaatt cccgctcacc
     1741 aaggcccgcc gtctgaccgg ccgcgcgttg ggcaatgtct cgcagctgta cttcgatgcg
     1801 cagaacgaca gcctgatcgg cgcgttccag taccccggcg tcctggccta cgtcggcgtg
     1861 ctctcgctga aggacggcag catccgccac ctggccgaca tcaagggtcc gatggcctac
     1921 cgggtggcat cgactgccta tgatccggcc agcaagactt tcttctacac agcggacaac
     1981 caggaattgc gcgacttgat ggaagtggac gtgcgcaacg gacggagccg aatgctgctg
     2041 aaggacgcgc gcatcggcga cctggcgttc gacaccgcgg atcgctcgtt gtggggcctt
     2101 cgccatctca acggctacgt cacgctggta cggatcccgt acccgtacaa ggactggacc
     2161 caggtgcaca cctggccgta tggcgacgat ccgttttcgt tggcggtatc gaaagatggc
     2221 agcatgcttt cgatgtcggt ggctacgatc gacggccata agatgttgcg catcttcaag
     2281 accgcggatt tgctggcggg cacggtcgag cctttcgccc atttcgattt tggccaggcc
     2341 acgcccgagg gtttcgtatt cacggccgat ggacgatatc tctacggcag ctcgtattac
     2401 accggcatct ccaacgtcta ccgttacgac atcgccacca agaagatcga tgcggtcagc
     2461 aatgccgaaa cgggtttctt tcggcccgtc cctttggccg acggcaagct ggttgcgttg
     2521 gagtacaccg gccagggctt cgtgcccgtg atgatcgatc ccgcgccctt gcaggacctg
     2581 agtgcgatca ccttcctcgg cacccggatt gccgaccagc acccggtggt gaaagcgtgg
     2641 ggtgtcgggt ccccggacaa ggtcccgctc aagtcgacaa tcacgggcaa gggcaagtac
     2701 cggccatcgc accagatccg gccccagacg cgctacccgg tggtcgaggg ctatcgtggc
     2761 ggtgtcgcac tcggctggca tttcgacttc accgacgcgc tgaacctgta caagcttggg
     2821 gtcaccgcgg ccgtgtccag gggcgggcag ggaggtgcgg gcgagcggtt gcacctgacg
     2881 gtcgactacc agggtatgaa ctggtatgcc cactactggc acaaccatgc cgacttctac
     2941 gacctgttcg gcccggttga aaccagcctc aagggcgacg cgctgatggg tggctacaaa
     3001 cgcgcgctgg ttttcgacag tccgcggcag atggacctgg acgtcggcgc ggcgtactac
     3061 acgggactcg caacgttgcc cggaagccag aacgtggccg cgcaggtcaa tgacatcttc
     3121 gccgcgcaca cgtcgctcat gtacaagaac cggcgtgcgt cgctcggcgg cctggaccgc
     3181 gaaaaaggcc tgagctggaa cgtcgatgcc acggcctatg tcgccggcgg caagaccgtg
     3241 ccgcaattgc gcggcgggtt ccagttcgga ttcctgttgc ccttgccgca tacctccctg
     3301 tggtcataca cctcggccgg tgtatccggg gggcagcgca gcagcgccct ggccaacttc
     3361 tatttcggcg ggttccacaa caactacgtc gacaaccagg accccaagca ataccgcggc
     3421 tacgacagtt tccccggttt cgaaatcgat gaaatcagcg gccatacgtt cgtcaagcag
     3481 atggtggaac tgaacctgac gccgctgcgc ttcgccgaag tgggcacgcc cgggttttac
     3541 ctcaaatggt tgcgcccggc gttgttcgcg ggaacgctgg tcaccgatcc gaaccatgcg
     3601 gcgtaccgtc ataccgtccg cgatctcggc gtccagatcg acatcgacat ctcggtgctc
     3661 aaccacctgc cgatgaccct gtcggcgggc tacgcgcgcg ggttcaacgg ccccgacggc
     3721 gactccgacg aatggatgct gtcgctgaag atcctgtgaa atgcacggcg ctccctccat
     3781 cctcgacctg ctgagcatca tcccggtcgg ccttctgccg gtcatcacgt tcctgctgct
     3841 gctggtttac ctcgacagct accagatggt gcggatgcgc ctggtggtgg cggtgttggc
     3901 ggccggaggc gtggtggcga tcgtttgcta cgcgctcaac ggcgggctga tgacgctgct
     3961 cggcatgaac ctgacgcact actcgcgctg ggtggccccg gtggtcgagg agaccctgaa
     4021 ggcgtcggtc gtggtgtggt tgttccgcac ccatcgcgtg ggattcctcg tggattcggc
     4081 gatcctcggc ttcgcggtcg gggcgggctt cgcggtggtc gagaacttct actacctgta
     4141 cctcgtgaac gacaccacgc tgaccgtctg gatcgtgcgc ggtttcggca ccgcgatgat
     4201 gcacggtggg gtggtggcga tcttcgcggt gttgtcgcag acgctcgccg aacgcagcgt
     4261 ggccgtgcgg cccattcatt gcctgcccgg gttggccgtc gctatttcga tccattccat
     4321 cttcaaccac ttctatgttt cgccggtgct gtccaccctc ggcgtgatgg tgatcctgcc
     4381 gccggtgctg tacctggtct tccgcatcag cgcataccgc acccaccgct ggctggaggt
     4441 cgacttcgac gaagacgccg aattgatgcg gatgatcgac tcgggccact tcgaccggac
     4501 ggccgccggc attttcctct cgggactgcg agacaaactc gacggtacgc tggtcgcgga
     4561 catgttctgc tacctgcgcg tctataccga actggcgctg cgcgccaagg gcctgctgct
     4621 cgcgcgcgag aacggtctcg atgtggagat ggaccgggaa acccgggaga aattcgcgga
     4681 actccgctac ctcgagcaca gcatcggcgt cgccgggcgc ctgacgatgc ggccgttcct
     4741 tcagatgacc accaaggatc tctggcagct ttacgtgctc ggcaagtgag ccgcgtccgc
     4801 gggcagccgc cgcaccagga tcagggtgac gtcgtcggcc gccgcaacgc ctgcggcgaa
     4861 gtcgtcggcc gctgccagca gtcgccggca cagcacggcc atagacaggt gctggccgga
     4921 acggaccact tcggcgacgc gccccggacc gaattcggcg ttgccggcgt caaggcgctc
     4981 gtacaacccg tctgaaatca gcgcgaggat gtcgccgggc gcaagctcga ggctggcgga
     5041 tgccggcacc tcctcgagtt ccatcacccc gaccgggaac gcgttcggtt gcagccattc
     5101 gcagcgatcg tcggcggcgt ggtaatgcag cagcggaccc tggcccgcgc actggtaacg
     5161 gacgcggtgc gcgccggcgt ccagcaagcc catgaacgcg gtgatgaagc ggtcgtcgga
     5221 caggtcttcg gtcaactggt tgttgacgtt gcggtacgcc tggtcgagat cggcaccgag
     5281 gcggaacgcc acccgcagca tgccttgcat ctgcatcgcc gacaacgccg gaccgaagcc
     5341 atggcccgtt gcatcgccca atagcaggaa cacgccttgg gagaatttca ccacgtcgag
     5401 catgtcgccg ccggcgtagg tggcgggctg gaagtggccg tgcaggtcgt agcccggcac
     5461 gctcggcatc tcggtcggca acgtgccgcg ctggatttca cgcgccactt ccacttcggt
     5521 gtgcagcgcg ctggccgcgc ccatggccgc atcgtgcgcc gcaccccgca ggaccagggc
     5581 gagttgcgcc gcgacggttt cggccaccag aacggcttcc gcgccgaacc cgcacgggga
     5641 cgcgtgcagc agttgcaata cgccgagcac ctcgcggccc gcgcgcagcg gcacgttcaa
     5701 ggctgcaccg acgctcgggg cattcgcgga atcgagcagc cggatatcca ccagctcggg
     5761 gtttccggac aggttgtcga gccgcagcat ttgcgcactt tgtgcacagc gcccggcgag
     5821 cccgacaccg ggctgcagcc gtggcagtgg ctcgcgtgcg ggcacctgca tgctgagttc
     5881 gccggacggc cgatcacgca gccacaaggc agccgcgcat gccccggtcc gcgtgcgcgc
     5941 aacctcgacg agcgcgacgg aggccggcgc gctgcccgag ccatccaggg tgcggatggc
     6001 ggcgcagaca tcttccagca atccagggtc cgccgacaga tcggcagcag ccatcgccct
     6061 gcggtccttc atccgctatg gcgccggaac gccaaaaaca ggccgcgcat cttcacgttg
     6121 tgcccccatc gccgcggttg gtctacgcgt ctgctagtgt cggcccccgg aacggcccgt
     6181 gcaagcgggc ggcgcgcacc tcgcggcatg gcgcttccga agtcccggca gtcaagcgca
     6241 caaagatcgc gcgtgatgcc gcaggtggtc ctcgatgaag ctttgcacga accagtagct
     6301 gtggtcgtag ccgggctgca tgcgcagggt gagtggctgc ccggtttctc ggcaggcttg
     6361 ctcgaacagt tccgggcgaa gctgttccgc gagatacgga tcttcctcgc cttggtcgat
     6421 gaggatcgtg ccggggaaac tcgcgccggc gcgcagcagc tcgcaggcgt catgccgctt
     6481 ccattcctcg cgatcggagc cgagataaag gatgaaaatc ttgcgatcgc cccacggcac
     6541 gcgggtcggc gccgcgatcg gtgacattgc ggaaagcgag cggtaccggc cgggatgccg
     6601 gagcgccagc gtcagcgcac catggccgcc catggaatgg cccatgattc cgcagcgcgc
     6661 aaaatccgcc ggaaatcgtc cggcaatcac cttcggcagc tcctcgacga cgtacgtctc
     6721 catccggaac cgcgacgaat acggcgcctg ggtggcgtcc acgtagaaac tcgcgccttc
     6781 gccgaattgc caatcgtccg ttgcacccgg gatgccggtg tcgcgcgggc tggtgtcggg
     6841 cgcgaccagc at
//
LOCUS       DASSNH010000199         7275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1519798, whole genome shotgun sequence.
ACCESSION   DASSNH010000199 DASSNH010000000
VERSION     DASSNH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519798"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFK00_07315"
     CDS             complement(<1..315)
                     /locus_tag="VFK00_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592795.1"
                     /translation="MQRKPGSLRNKLMVASALVATAVLATPAAMARGYHHNGDDTAKV
                     VGALVVGAVIGGVLASSGHHDNYYRGGYAYPARTYYPSNYYGSYPAYSYNSYPTYSYN
                     TYP"
     gene            complement(446..1051)
                     /locus_tag="VFK00_07320"
     CDS             complement(446..1051)
                     /locus_tag="VFK00_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592796.1"
                     /translation="MRKAAVVIKRNSKPARKSDKTVRKAKAEATTTRKTAGRKAAKPA
                     NAKAAALARTGKKGGTKAKAAKPAAAKSKTAARKPVAKKPAAKKAAAPATTRTAARPA
                     SVKTAAKAVAAARASASKRRPPSYIPRSRFNDDWMSAGPVELPRQPNPAAHTPTRKPP
                     KPRAPKPKAISHEQAVANLKALLESRKRKDRGGEASASKGA"
     gene            1578..1796
                     /locus_tag="VFK00_07325"
     CDS             1578..1796
                     /locus_tag="VFK00_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592797.1"
                     /translation="MRSFDDERGGHWQAALMEASFGNVLLVLSRIGAPDVLQTALDSA
                     NFHEAEDWLAAAGEDQLRTMLAEARPWD"
     gene            complement(1805..2263)
                     /locus_tag="VFK00_07330"
     CDS             complement(1805..2263)
                     /locus_tag="VFK00_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592798.1"
                     /translation="MLSSRVAAAAPFATLGVAAVLAGGATAAAIAYHPSEHLVWMVAY
                     LVLVVGVMQVVFGAGQAWLAGRPPSGVVAWGQWILFNLGNAGVIGGTLCNRPEIVAAG
                     TLLFVVAIAWFLFGVGHCRMRAWGNAYRVLLALILLSACVGLVISFVSHA"
     gene            complement(2257..3288)
                     /gene="lipA"
                     /locus_tag="VFK00_07335"
     CDS             complement(2257..3288)
                     /gene="lipA"
                     /locus_tag="VFK00_07335"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7592799.1"
                     /translation="MGEPNTPRPLPGSKFTSPQGTRAVKDGIRPSKLSRLPDADRKPP
                     WLRVKLPAGAKYEEIRDIVRTHRLSTVCAESKCPNIAECWGRGTATLMLMGSVCTRAC
                     KFCSVSTGNPHGWLDPMEPASVADAVALMNLKYVVLTSVDRDDLPDGGAGHYAACIRA
                     IHGRTPVTAVEALTPDFAGRHEMVAKVLDAGLATYAQNIETVERLTHPVRDPRAGYRQ
                     TLDILKFAKGYAPGTVIKTSMMLGLGETDDEIDRTLDDIRAADVDVVTMGQYMRPTRN
                     HLPVERFVTPEQFHRYRQMALAKGFLEVVSGPLVRSSYRAEQVLEHNNVGLDAAIMGG
                     IRQSASMRC"
     gene            complement(3483..4094)
                     /locus_tag="VFK00_07340"
     CDS             complement(3483..4094)
                     /locus_tag="VFK00_07340"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7592800.1"
                     /translation="MLLTIAIVAVTCVVSWMAFKDARLLDRLLLWPPAIQRKHEYWRL
                     LSYGLVHADFQHLLFNMITLFFFGRAMAGLYAQYLGPLGFLWFYLGGLLVSILPTYIA
                     NRRNPNYRSLGASGAVSAVLFAFILMAPWSMILVFVIPVPAILYAVAFVAYSIWADRH
                     GGSYINHNAHMWGAAYGIVFTVIMAPPIIPHFLQQLRNPHFGF"
     gene            4213..5955
                     /locus_tag="VFK00_07345"
     CDS             4213..5955
                     /locus_tag="VFK00_07345"
                     /inference="COORDINATES: protein motif:HMM:NF023643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N4-asparagine amidase"
                     /protein_id="HET7592801.1"
                     /translation="MSQASRTRSPRLPILLATTAFVAMSAAAAMAPEPIAGPPIAIGE
                     TGVAVADPLVPVPPETPCVVQLYTADSPTGTFDDYANHPFQYTPPAGCEGPWNKVVFK
                     ADYNVTAGRQFDRTASVWIGGANIYFGTTQEPSSSRSPSWHVERDVTDLSALLETAQP
                     GNAAVYNIYDSTYNGSITGSAELDFYPATPEFPAPRVPDQVLSLSSDPAGVYANIYTD
                     ETPLSRTFTLPSNIDRAYIDVIAEPQSSDEFWYSCFPEPYDSELGNCGGTGFREAEVA
                     IDGNAAGIVPISGWIYTGGIDPYLWRPIPGVQTLEFEPYRVDITPFAGLLDDGQPHTV
                     SVSVTTGDPAFENQSQYFATAATLLLYQDPEATQLSGGLSEVVDSGVHPVQTNTAGPD
                     GTTFGFKVTSHHSLVTRGWLDTPHGRATTSVVQDVRFSNDQSVVATDTRFDQSITQTS
                     TALQRSYGRPLEGPRNTTTNWSFPLAMDINLQLADDGSETQATTVNQGFSLQYRETGG
                     PGGQYTFNRNQSDSAADTLNFDPDGNFTGPTGQQASQSYTFTDSRGACYSRTVEAVAG
                     AVTSVVDGSACSQR"
     gene            6111..6983
                     /locus_tag="VFK00_07350"
     CDS             6111..6983
                     /locus_tag="VFK00_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592802.1"
                     /translation="MSTSLRSRPFALGFAAGVLAACIALPVFADDATAVSFQANNPWT
                     QEVGSVDRFHESRDYTVQVGAGKTLQINLVTRDPNVFFKIRNETRDEQLVDTFQTGAT
                     TWSTQNTDAATYTIHVYVDPATMQRGEEAKYALQIGQYGAADMRSPTTTVTFQDNKPW
                     VQAVGTLDSQASARDYAVAIGAGETLAVNLVTHNPKVYFKVENQASGQILVDSATSHN
                     DKWSMPVDAAATFTVSVYADPAALPPGSRAGYALQFGHYVQNNLSPVAAGTAASPASA
                     TSAPVPAASAGSAG"
BASE COUNT         1223 a         2575 c         2285 g         1192 t
ORIGIN      
        1 cgggtaggtg ttgtacgagt acgtcggata gctgttgtac gagtacgccg gatagcttcc
       61 gtagtagttc gacggatagt aggtccgcgc cggataggcg tacccgccgc ggtaatagtt
      121 gtcgtggtgg ccggagctcg ccagcacgcc gccgatcacg gcgcccacca ccagcgcacc
      181 caccactttc gcggtgtcat cgccgttgtg atggtagcca cgtgccatgg cggccggcgt
      241 ggccagtacc gccgttgcga ccagcgcgga ggcgaccatc agcttgttgc gaagcgaacc
      301 gggtttacgt tgcatggctt tcggccctcg gtgagacgcc cgcagtatcc cacatcgagg
      361 taaatctaaa ctgaagagtt ttgttatgga agcgctaacc ttcggggccg cgtcgcacgc
      421 ggcaccgaag attcggccat tcggcttacg cgcccttgga tgccgaggct tcgcccccgc
      481 gatccttgcg cttgcgcgat tccagcagcg ccttcaggtt cgccaccgcc tgctcgtggc
      541 tgatcgcctt cggcttcggc gcacgcggct tgggcggctt gcgagtcggc gtgtgggcgg
      601 ccggattggg ctggcgcggc agttcgaccg ggccggccga catccagtcg tcgttgaagc
      661 gcgagcgcgg aatgtaggac ggtggccgcc gcttgctggc actggcccgg gccgcggcga
      721 ccgccttggc agccgtcttg accgaagcgg gacgcgccgc cgtcctggtg gtcgccggcg
      781 ccgcagcctt cttcgccgcc ggtttcttcg cgacaggctt cctggccgcc gtcttcgact
      841 tggccgcggc cggcttcgcc gccttggcct tggtgccgcc tttctttcca gtcctggcga
      901 gcgcggctgc cttggcattc gccggcttgg ccgccttccg gcccgccgtc tttcgggtag
      961 tcgtcgcctc ggccttcgcc ttgcgaaccg tcttgtcgct cttgcgagca ggcttgctgt
     1021 tgcgtttgat gaccactgca gctttcctca tgtagtcgct ccgcgcctcc ggcccacggg
     1081 gccggaactt gcgagcttga tcaagcgaga aggaacttcc ggccgcgacg gcgcctgcta
     1141 ggacatgtcg tcctggaacc ggcgcccatc gacgtgcctg acgttcagct ccgtcggttc
     1201 aatcccctcg aggcaacgca cattcaccgc cgccatggcg cggccctcct ctccccttcg
     1261 gcaacggcgc cacaccgggc agaaatgatg ggcgatggca tgcttgttga accggtaggt
     1321 gcccatctcc gcgtccggcg tgacagccgc agtccttcgc tgcagaaatc acaacaggta
     1381 gccttttggc cggcaatgcg agcagttgca ttccaccacg gcaccggttt cgtcctcgaa
     1441 agcggtccgg ccgcagtggc acccaccact ccaagtcgtc atgcgcgtat cgtcgggcac
     1501 catccattgc tgattatcgc atgaaacggg gcttctccgc acgtgtatcc tgttgatccc
     1561 cctctcgaag gtttgcaatg cgcagtttcg acgacgaacg gggcggccac tggcaggccg
     1621 cgctgatgga agcttccttc gggaacgtgc tgctggtgtt gagccggatc ggcgcgccgg
     1681 acgtgttgca gaccgccctg gactcggcca atttccacga agccgaggac tggctggccg
     1741 cagccggcga agaccagctg cgcacgatgc tggcggaggc ccggccgtgg gactgagccg
     1801 cccgctatgc gtgtgaaacg aacgagatca cgaggcccac gcaggcactg aggaggatca
     1861 gcgcgagcag gacgcggtag gcgtttcccc aggcacgcat gcggcaatgg ccgacgccga
     1921 acaggaacca tgcgatcgcc accacgaaca gcagcgtgcc cgcggccacg atctccggcc
     1981 ggttgcagag ggtcccgccg atcaccccgg cgttgcccag gttgaagagg atccactggc
     2041 cccacgccac gacgccggat ggcgggcgcc cggcgagcca tgcctgtccg gccccgaaca
     2101 ccacctgcat cacccccacc accagcacca ggtaggccac catccagacc aggtgctcgc
     2161 tcgggtggta tgcgatcgcg gcggcggtcg cgccgccggc aaggaccgcg gccacgccta
     2221 gcgtcgcgaa cggcgccgcc gcggcgacgc gactgctcag catcgcatcg aggccgactg
     2281 gcggattccg cccatgattg cggcatcgag gccgacgttg ttgtgctcga gcacctgctc
     2341 ggcgcgatag ctggagcgga ccagcggtcc cgacacgacc tccaggaaac ctttcgcgag
     2401 cgccatttgc cggtaacggt ggaactgctc gggcgtcacg aaacgctcca ccggcaagtg
     2461 gttcctggtg gggcgcatgt attggcccat cgtcaccacg tcgacgtcgg ccgcccggat
     2521 gtcatcgagg gtgcggtcga tctcgtcgtc cgtctcgccg agacccagca tcatgctggt
     2581 cttgatcacc gtaccgggcg cgtagccctt ggcgaacttc aggatgtcca gcgtctgccg
     2641 gtagccggca cgcgggtcgc gcaccgggtg cgtcagtctt tctacggttt cgatgttctg
     2701 tgcgtaagtg gctaatcctg catccagaac cttggccacc atctcatgcc gcccggcgaa
     2761 atcgggggtc aacgcctcga ctgccgtgac cggcgtgcgg ccatggatcg cccggatgca
     2821 cgccgcgtag tgcccggccc cgccgtccgg gaggtcgtcg cggtcgaccg aggtcagcac
     2881 cacgtatttc aggttcatca gcgcgaccgc gtcggccacg ctggccggct ccatcggatc
     2941 cagccagccg tgcgggttgc cggtcgagac cgagcagaac ttgcatgcac gggtgcatac
     3001 cgagcccatc agcatcaggg tcgcggtgcc gcggccccag cattcggcga tgttcggaca
     3061 tttggactcc gcgcacacgg tggacagcct atgcgtgcgc acgatgtcgc ggatttcctc
     3121 gtacttggcg ccggccggca gcttcacccg cagccagggc ggcttgcggt cggcgtcggg
     3181 aagtcggctc agtttgctcg gccgtatgcc gtccttgacc gcacgggttc cctgcggact
     3241 ggtgaacttg ctgcccggca acgggcgcgg cgtgttcggt tcgcccatcg attttcccat
     3301 tcaatgattg tcgacgcacg agttcgcgcg cccagccgga cattgtagcg gcctgcgcgg
     3361 agggcggaac ccgcgccgcg gcttccgtcg attcttccgc gagatgtggt ggaagcggct
     3421 gcgccgatcc caccctacga cctggcatcg acacgcgctc cagcgcgcag ccgaagcgtt
     3481 tttcagaacc cgaagtgcgg attccgaagt tgctgcagga agtgcgggat gatcggcggg
     3541 gccatgatca ccgtgaacac gattccgtaa gccgcgcccc acatgtgggc gttgtggttg
     3601 atgtagcttc cgccatgccg gtccgcccag atcgaatagg cgacgaacgc caccgcgtac
     3661 aggatcgccg gcaccgggat cacgaacacc aggatcatcg accacggcgc catcaggatg
     3721 aaggcgaaca gcaccgccga caccgcgccc gacgcgccga ggctgcggta gttcgggttg
     3781 cggcgattgg cgatgtacgt cggcaggatc gacaccagca ggccaccgag atagaaccac
     3841 aggaatccca gcggcccgag atactgcgcg tacagccccg ccatcgcccg tccgaagaag
     3901 aacagcgtga tcatgttgaa cagcaggtgc tggaaatcgg cgtgcaccag cccgtagctc
     3961 agcagccgcc agtattcgtg cttgcgctgg atcgccggcg gccacagcag cagccggtcg
     4021 agcagcctgg cgtccttgaa cgccatccac gagacgacgc aggtgaccgc gacgatggcg
     4081 atcgtcaaca acatgccggg atccaggatc gggcgcgcac gtgcgcagga cccgcgactg
     4141 taacgtttcg ccgcacgcgg tgctaggctg gcgcagccgg accggggagg gacacacttc
     4201 aaggagaacg ccatgtccca agcgtcgcgt acgagatctc cgcggctccc catcctcctg
     4261 gccaccaccg ccttcgtcgc gatgagcgcc gccgccgcaa tggcgccgga gcccatcgca
     4321 ggcccgccga tcgcgatcgg cgaaacgggc gttgcggtcg ccgacccgct ggtgccggtg
     4381 ccgcccgaaa cgccctgcgt cgtgcagctc tacaccgccg actcgccgac cggcaccttc
     4441 gacgactacg ccaaccatcc gttccagtac accccgcccg ccggttgcga gggaccctgg
     4501 aacaaggtgg tgttcaaggc cgactacaac gtcaccgccg gccgccagtt cgatcgcacc
     4561 gcgagcgtct ggatcggcgg cgccaacatc tacttcggca ccacccagga gccgtcgtcg
     4621 tcgcgcagcc cgagttggca cgtcgaacgc gacgtgaccg acctgtccgc gctgctcgag
     4681 acggcgcaac cgggcaatgc cgccgtctac aacatctacg acagcaccta caacggttcc
     4741 atcaccggca gcgcggaact cgacttctac ccggcgacgc cggagttccc ggcgccgcgg
     4801 gtgccggacc aggtgctgtc cctgtcgtcg gatcccgctg gcgtgtatgc caacatctat
     4861 accgacgaga cgccgctgtc gcgcaccttc acgctgccta gcaacatcga ccgcgcctac
     4921 atcgacgtga tcgccgagcc ccagtcgagc gacgagttct ggtattcgtg cttcccggaa
     4981 ccctatgaca gcgaactcgg caactgcggc ggcaccggct tccgcgaggc cgaggttgcg
     5041 atcgacggca acgcagccgg catcgtcccg atctcgggct ggatctacac cggcggcatc
     5101 gacccctacc tgtggcgacc gatcccgggc gtgcagaccc tcgaattcga accctaccgc
     5161 gtcgacatca ctccgttcgc cggcctgctc gacgacggcc agccgcacac ggtgagcgtc
     5221 agcgtcacca cgggcgatcc ggccttcgag aaccagagcc agtacttcgc gaccgccgcg
     5281 accctgctgc tctaccagga tccggaagca acccagctca gcggcgggtt gtcggaggtc
     5341 gtcgactcgg gcgtccatcc ggtccagacc aacaccgccg ggccggacgg cacgacgttc
     5401 gggttcaagg tcacctcgca ccacagcctc gtcacccgtg gctggctgga tacgccgcac
     5461 ggccgcgcca cgaccagcgt ggtccaggac gtccgcttca gcaacgacca gagcgtcgtc
     5521 gccacggaca cccgcttcga ccagtccatc acccagacca gcacggcgct ccagcgctcc
     5581 tacggacggc cgctcgaagg tccccgcaac acgaccacga actggtcgtt cccgctggcg
     5641 atggacatca acctgcagtt ggcggatgac ggcagtgaaa cccaggccac caccgtcaac
     5701 cagggctttt cgttgcagta ccgcgaaacg ggtgggcctg gcggccaata caccttcaat
     5761 cgcaatcaga gcgacagcgc cgcggatacg ctgaacttcg atccggacgg caacttcacc
     5821 ggcccgaccg gccagcaggc gagccagagc tacaccttca cggactcgcg cggcgcctgc
     5881 tacagcagga cggtcgaagc ggtcgccggt gcagtgacgt cggtcgtcga cggcagcgcg
     5941 tgcagccagc ggtaacctgc ccgccatcga ggatgcgcgg ccgccgcgct tgcggcggcc
     6001 gcggctacgc cgcaagatag ccgaatgctg ccgagagcgc cggggacgct ccacgctcga
     6061 cccgtccctt cgttccgcgc gagcaaccca ttccagcgcg aggattcccc atgtccactt
     6121 ccctccgttc ccgccccttc gccctcggct tcgccgccgg cgtcctggcg gcgtgcatcg
     6181 cactgcctgt attcgccgac gatgccaccg cggtcagctt ccaggcgaac aatccgtgga
     6241 cgcaggaagt cggcagcgtc gaccgcttcc acgagtcgcg cgattacacg gtccaggtcg
     6301 gggcgggcaa gaccttgcag atcaacctgg tgacccgcga cccgaacgtg ttcttcaaga
     6361 tcaggaacga aacccgggac gagcaactgg tcgacacctt ccagaccggc gccactacct
     6421 ggtccacgca gaatacggac gcagcgacct acacgatcca cgtctacgtc gacccggcca
     6481 cgatgcagcg cggcgaggaa gccaagtacg cgctccagat cgggcagtac ggcgccgcgg
     6541 acatgcgctc gccgaccacg acggtgacct tccaggacaa caagccctgg gtgcaggccg
     6601 tcggcaccct cgattcgcag gccagcgcgc gcgactacgc cgttgcgatc ggcgccggcg
     6661 agaccctcgc cgtgaacctc gtgacccaca atcccaaggt ctacttcaag gtcgaaaacc
     6721 aggccagcgg ccagatcctc gtcgacagcg ccaccagcca caacgacaag tggtccatgc
     6781 ccgtggacgc ggcggcgacc ttcacggtca gcgtgtacgc cgaccccgcg gcgcttccac
     6841 ccggcagtcg cgcgggttat gcgctgcagt tcggccatta cgtccagaac aacctgtcgc
     6901 ccgtcgccgc ggggacggcc gcgtcgcccg cttccgcgac cagcgcgccg gttcctgctg
     6961 catccgccgg cagcgcaggc tgagcgacgg ctcgcgatcc ggctgatcga tccgcgcatc
     7021 gcgagcacct tccgcgtcaa cgaccgtgcc ttccagcacg gtcgttttcg tgcgcacggc
     7081 ggtaagctcg cgggctttgt ccttggggaa ccgccgatgc acaagcctct tctcgcgacc
     7141 gcgttcgccg tcgccgcctg tttcgcctgt gcggcttcgc tgcccgcgca cgccgagtcc
     7201 gccaaggcca aggccgacag cagttcggac ttccagttgc cgccgttgcc tgctgccgcg
     7261 agcaagtcgc agagc
//
LOCUS       DASSNH010000200         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1271870, whole genome shotgun sequence.
ACCESSION   DASSNH010000200 DASSNH010000000
VERSION     DASSNH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271870"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFK00_07355"
     CDS             complement(<1..1116)
                     /locus_tag="VFK00_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET7592803.1"
                     /translation="MPEIIRPELPAPPGPTRARITAAWLRDETEAVNELLNLAQAPAD
                     ERAAVESHAAALVKRVRAKQDERSAVAAFMQQYDLSSTEGVLLMCIAEALLRIPDSET
                     ADRLIRDKLVEADWKKHLGTSKSVFVNASTWGLMLTGHIVTLPDTTRRDYQAALRRLL
                     NRAGEPLVRLAVRQAMRIMGQQFIMGRTIKAALGRAQRSDNAAYRYSFDMLGEAALTA
                     PDAERYLRAYRDAIDTLGNHPKAGDVLAAPSVSVKLSALHPRYEVADRERVHAELTPR
                     VLELAQLAMARGIALTIDAEEAARLELSLDVIHAVFTDPSLAGWNGFGLAVQAYQKRA
                     PFVIDWLAETARNAGRRWCVRLVKGAYWDAEIKLAQEQ"
     gene            1268..1762
                     /locus_tag="VFK00_07360"
     CDS             1268..1762
                     /locus_tag="VFK00_07360"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HET7592804.1"
                     /translation="MITRLPGVAGAGQVTLLLAPNRALNRRQVRRVVWELSIAALLVA
                     AVAAWGGDVFAPLFTLIVIPVVAIAFWLVWRSGNRGERITLDTDNVRIEQVPPSGRAA
                     STFPAAWTRVRVLERGDGHRHVVLTAHGRAQEVGQCLGDDERMQLSRWLGNLLSGYSG
                     WRDG"
     gene            1932..2858
                     /gene="coxB"
                     /locus_tag="VFK00_07365"
     CDS             1932..2858
                     /gene="coxB"
                     /locus_tag="VFK00_07365"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7592805.1"
                     /translation="MTMSSSNCKRWIQGTAFAFGGMLLSMTAAANPRPWQLNLDAGVT
                     PLSNHIWQLHMAAFWVCVGLGVIVFGVMFAAMFRFRKSRGAVAATWSHNTKLEFMWTL
                     LPVLILIGLAAPATIVTRELYDASGAQMTVKITGYQWLWRYDYVSYDGKPITKVPTIY
                     SRLAWDSNTARQLDSGIDPNSIVAPDGFHDYLLDVTHPLVIPAGVKVRFLITGGDVIH
                     GWWVPDFAAKRDAIPGIMNDAWATVEKPGIYRGQCYVLCGQDHSAMPVVVKVLPRDQF
                     NAWLAAQQNAATLDKASRTAEVAPPKPAPAKG"
BASE COUNT          483 a          973 c         1061 g          538 t
ORIGIN      
        1 ctgttcctgg gccagcttga tctcggcatc ccagtacgcg cccttgacca gccgcacgca
       61 ccagcggcgg ccggcattgc gcgcagtctc ggccagccag tcgatcacga acggcgcgcg
      121 cttctggtag gcctgcacgg caaggccgaa gccgttccag cccgcgagcg acggatccgt
      181 gaataccgca tggatcacgt ccagcgacag ttccaggcgc gcggcttcct cggcatcgat
      241 cgtgagcgcg atgccgcgag ccatggcgag ttgcgcgagt tccagcaccc gcggcgtgag
      301 ttcggcatgc acgcgctcgc ggtcggccac ttcatagcgc gggtgcagcg cggacagctt
      361 caccgacacg gacggcgcgg ccagcacgtc tccggccttc ggatggttgc cgagggtatc
      421 gatggcgtcg cggtaggcgc gcaggtaacg ctcggcgtcg ggcgcggtca gcgcggcttc
      481 gcccagcatg tcgaacgaat agcggtaggc ggcgttgtcg ctgcgctgcg cgcgcccgag
      541 cgcggccttg atcgtgcggc ccatgatgaa ctgctggccc atgatccgca tcgcctgccg
      601 caccgcgagc cgcaccagcg gttcgcccgc gcggttcagc aggcggcgca gcgcggcctg
      661 gtaatcgcgg cgggtggtgt cgggcagcgt gacgatgtgc ccggtgagca tcagccccca
      721 cgtggaagcg ttgacgaaca ccgacttgct ggtgccgagg tgcttcttcc agtcggcttc
      781 gacgagcttg tcgcggatca gccggtcggc ggtttcgctg tcggggatcc gcaacagcgc
      841 ttcggcgatg cacatcaaca gcacgccttc cgtgctcgac aagtcgtact gctgcatgaa
      901 ggcggcgacc gcgctccgct cgtcctgctt ggcgcgcacc cgcttgacca gtgcggcggc
      961 gtggctttcc accgcggcgc gttcgtcggc gggtgcttgc gccaggttca gcaattcgtt
     1021 gacggcctcg gtctcgtcac gcaaccatgc cgcggtaatg cgcgctcggg tcgggcccgg
     1081 cggagcgggc aattcgggtc ggatgatttc gggcatcgga gcaggctgcg acgtgaccgc
     1141 ccattgtacg tcgctgcatc cgcgcggcca atgatgcgtg gcacaagtca tggacaggcg
     1201 cgggcttatt cttgccgtgc cggagcgggc gccttatgat tccctgactg cggtaccgcg
     1261 aatgtccatg atcacacgtc ttccgggagt cgctggagcc gggcaggtga cgttgctgtt
     1321 ggcgccgaac cgggcgctca atcgtcgcca ggtgcggagg gtggtgtggg agttgagcat
     1381 cgcggcattg ttggttgcgg cggttgcggc gtggggcggt gacgtgttcg cgccattgtt
     1441 cacgttgatc gtgatcccgg tggtggcgat cgcgttctgg ttggtgtggc gcagcgggaa
     1501 tcgcggtgaa cgcatcacgc tcgacacgga caacgtccgg atcgagcagg tgcccccgag
     1561 tggccgtgcc gccagcactt tcccggcggc gtggacgcgg gtgcgggtgc tggaacgcgg
     1621 cgacgggcat cggcacgtgg tgctgaccgc acatggtcgc gcgcaggaag tggggcagtg
     1681 cctcggggat gacgaacgga tgcaattgtc gcggtggttg ggaaacttgc tgtccggcta
     1741 cagcggctgg cgtgatggtt gaggaaggcc tgggtcactt gtcgttgcag cgccggcgac
     1801 gaggtcatgc ttcccggtcc cgccggaccc ttgcccgcgt ggcgacggtt cggcagcccg
     1861 cctgaccgaa agacgggcgg cgaaacgatg agacgaacca caacacagat tccaagcgag
     1921 ccgggtgtgc gatgacgatg agttccagca actgcaagcg gtggatccag gggacggcgt
     1981 tcgccttcgg gggcatgctg ctgtcgatga ccgccgccgc gaacccgcgc ccgtggcaat
     2041 tgaacctcga tgccggggtg acgccgctct cgaaccacat ctggcagctc cacatggcgg
     2101 ccttctgggt gtgcgtcggc cttggcgtga tcgtgttcgg cgtcatgttt gccgcgatgt
     2161 ttcgattccg caaatccagg ggcgcggtgg ccgcgacctg gagccacaac accaagctgg
     2221 aattcatgtg gacgctgctg ccggtgctga tcctgatcgg cctcgcggcc ccggccacca
     2281 tcgtcacgcg cgagctctac gacgccagcg gcgcgcagat gaccgtcaag atcaccggct
     2341 accagtggct gtggcgctac gactacgtca gctacgacgg caagccgatc accaaggtgc
     2401 cgacgatcta ttcgcgtttg gcatgggaca gcaacacggc ccgccagctc gattcgggca
     2461 tcgacccgaa cagcatcgtc gcccccgatg ggttccatga ttacctgctc gacgtgaccc
     2521 acccgctggt gatcccggcc ggcgtcaagg tgcgcttcct gatcaccggc ggcgacgtga
     2581 tccacggctg gtgggtgccg gatttcgcgg ccaagcgcga tgcgatcccc ggcatcatga
     2641 acgatgcctg ggccaccgtg gagaagccgg gcatctatcg cggccagtgc tacgtgctgt
     2701 gcggccagga ccacagcgcg atgccggtgg tcgtcaaggt gctgccgcgt gaccagttca
     2761 acgcgtggct ggcggcgcag caaaacgccg cgaccctcga caaggcatcg cgcaccgccg
     2821 aggtggctcc gccgaaaccg gccccggcca agggctgacg acggcaccga gatcagagca
     2881 gcagcaggca ttccagagga ttcgcatcat gtcgagcacg gcgcacgcgg ttcattacga
     2941 agaacaccac cagagcttct tcgagcgctg gttcatgtcc accaaccaca aggacatcgg
     3001 gacgctgtac ctgctgttct cgttcctgat gctgttcgtg ggcggcggct tcgcg
//
LOCUS       DASSNH010000201         5382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_425971, whole genome shotgun sequence.
ACCESSION   DASSNH010000201 DASSNH010000000
VERSION     DASSNH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425971"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..997
                     /locus_tag="VFK00_07370"
     CDS             32..997
                     /locus_tag="VFK00_07370"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7592806.1"
                     /translation="MKASLRTASAPPSSQPSGTVIPGKPPRSVKANPSVLKVTPLGEG
                     DTIQVQTLTRAHLLQPLPVAILDTLGSRVLPHLLGVEEPPPAGPSPPPAAPPSSAPAP
                     AAPATAAIPLKLAADTAVPPRRGAVYRVTKDNGTVLYTNIASLAKGSHATRLFTYIVE
                     CYACNLHSKVDWSTVPLHLAAYRDDIRSAAESTGVDVALLRAVIHAESGFNSRALSYK
                     GAQGLMQLMPGTATELGVGDAFDPGQNISGGARYLAMLLRDYHGDVQLAAAAYNAGEG
                     AVTKYGGVPPYAETQVYVKRVALLYQRYRKALAGESTLAVARGAG"
     gene            complement(1209..2015)
                     /locus_tag="VFK00_07375"
     CDS             complement(1209..2015)
                     /locus_tag="VFK00_07375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7592807.1"
                     /translation="MARPAVTVAIRAARAAGSVILRYMNRLDSVVVTEKRHLDFVSEV
                     DRQAEGEIVRELRRAYPDHAILGEESGASGKAGARFTWAVDPLDGTHNYLRGIPQFAV
                     SIAQLDRGEPVHAVILDPLRNEIFTASKGDGAFLNDRRIRVGRRDSLDGAMLATGFPY
                     RQRQHLDAQLAMTREVLKRAEDIRRAGSAALDLAWTAAGRLDGYFETGLEVWDMAAGA
                     LLVREAGGRYCDLVGRDGLPQSGNIVAANHMLAEQIVEAIAPALTSALKR"
     gene            2115..2924
                     /locus_tag="VFK00_07380"
     CDS             2115..2924
                     /locus_tag="VFK00_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7592808.1"
                     /translation="MNQSTASIPPQPQTIRFVLSRTSHPGNIGAAARAIRTMGFWRLV
                     LVAPQRYPDPQATAMAAGADDVLAHLAVADSVTDAVGDCERVYGLSARRRGVNLPELG
                     PRAAVAEIAPLVRQGREVALLFGNERSGLDNEELSVCHAMVRIPSVEEFSSLNLAQAV
                     QLMAWELRMAMLGEVPAAPPAHDDPATADQMERFFEHLFATLHAIDFHKGRSATTIEQ
                     RLRRLFHRAMPDARELRILHGVLADAERMARLAKGEIPPPLRGDPLRPRRG"
     gene            complement(2951..4309)
                     /locus_tag="VFK00_07385"
     CDS             complement(2951..4309)
                     /locus_tag="VFK00_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7592809.1"
                     /translation="MLTELGLVLLLALCNAFFALAEIALIAARKSRLRHMARTSTRAQ
                     VALRLAQRPDRFLSTVQVGITLITLITGAAAGAAVSDRIRQWLIDLGMPAAANASLVI
                     GLVLGFILVTTINITVGELVPKRVALIAPERIAQVVAFPMQVLSWLLTPFVWVLTALS
                     ELVLRLLGMHKVRREKVSEEEIRMLVAEGAEQGVLDPDERNMVNRVLRLGDRTVDSLM
                     TPRPRIAWLDAAAPLEENLAVMHATPYSRYPVYRADEDEVLGVLEVKSLLQAMSQDPR
                     KGMAPAKPDLFRKLAKPLFVPATARALGILDEFRDAGMPFALVVDEYGDIEGLVTVND
                     LLNAVVGRATHADGKASHGGSAVPRDDGSWLVDGSLSTDDLRELLQIDELPNEKDGDY
                     YTLAGMLIDALGHIPAVGENTVWQQWSFEVVDLDGVRIDKVLVTPLARSEAEDEAGLG
                     GG"
     gene            4418..5047
                     /locus_tag="VFK00_07390"
     CDS             4418..5047
                     /locus_tag="VFK00_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pit accessory protein"
                     /protein_id="HET7592810.1"
                     /translation="MFSLQTMFGKGDRFYDLLEASAGAARDGAKALQGLLEAHGEPML
                     LDGFRAARRREKELATNISEELINTFVTALDREDIEAMSSALYRIPKTIEKFAERYVI
                     VADRVEGIDFASRASLLMSATEGVFEMVRELRNGLSIDRMRKLQDRLQAVEAEADRML
                     LEPYRALYLGSNDPIRIMLAKDLFELLEKAVDRCRDVGNVIYSIVLKNS"
     gene            5259..>5382
                     /locus_tag="VFK00_07395"
     CDS             5259..>5382
                     /locus_tag="VFK00_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7592811.1"
                     /translation="MSLGLLTAVIVIALVFTYINGFHDTANSIATVVATKVLSPG"
BASE COUNT          871 a         1803 c         1847 g          861 t
ORIGIN      
        1 cggcgactgg aacacatcgc cggcgcggcc cgtgaaggcg agccttcgca cggcgtcggc
       61 gccgccctcc agccagccca gcggcaccgt gatcccgggc aagccgccaa ggtccgtcaa
      121 ggccaatccg tcggtcttga aagtgacgcc gctcggcgaa ggcgacacca tccaggtcca
      181 gacgttgacc cgggcgcacc tgttgcagcc attgccggtc gcgatcctgg acacgctggg
      241 cagccgggtc ctgccgcacc tgctcggcgt cgaggaaccg ccgcccgccg gtccgtcacc
      301 tccacctgcc gcacctcctt ccagtgcgcc tgcgcctgcc gctcccgcga ccgccgcgat
      361 tccgttgaag ctcgcggccg atacggccgt tccgcctcgc cgcggcgccg tgtatcgggt
      421 gacgaaggac aacgggacgg tgctctacac caacatcgcg tcgctggcga agggctccca
      481 cgccacccgg ctgttcacgt acatcgtcga gtgctatgcg tgcaacctgc attcgaaggt
      541 cgactggagc accgtgccgc tgcacctcgc cgcttatcgc gacgacatcc gcagcgcggc
      601 cgagagcacg ggcgtcgacg tcgcgctgct gcgcgcggtg atccacgccg aatcgggctt
      661 caattcgcgc gcgctttcgt acaagggcgc gcaggggctg atgcaactga tgccgggcac
      721 cgcgaccgag ctcggcgtgg gcgatgcgtt cgatcccggc cagaacatct cgggcggtgc
      781 gcgctatctg gcgatgctgc tgcgtgacta ccacggcgac gtgcaactcg ccgcggccgc
      841 gtacaacgcc ggggaagggg cggtgacgaa gtacggcggc gtgcctccgt atgccgagac
      901 gcaggtgtac gtgaagcggg tggcgctgct gtaccagcgc tatcgcaagg cgctcgccgg
      961 agagtccacg ctggccgtcg ccagaggcgc aggctgagga acggcgcctg cgcgatcgtt
     1021 cgttgggctt cgctgcgctc agcaccaacc tgcaagagcg gcttccgtag gttgggttga
     1081 tcccggacgc agtccgggag aagcccaaca ttcatcgcac cgggatgtgc gatgggagcc
     1141 gtgaacctcg tgcggcttcc cgggatcacc ttcggggtgg gcgccgaccc gcaacagccg
     1201 tgcgtggatc agcgtttgag cgcggacgtc agggccgggg cgatggcctc gacgatctgc
     1261 tccgccaaca tgtggttcgc ggcgacgatg ttgcccgact gcggcaagcc gtcacggccg
     1321 accaggtcgc aatagcggcc gccggcttcg cgcaccagca gggcacccgc ggccatgtcc
     1381 cacacttcga ggccggtttc gaaatagccg tccagacgtc cggcggcggt ccaggcgaga
     1441 tccagcgccg ccgaacccgc ccggcggatg tcctcggcgc gcttcagtac ctcgcgggtc
     1501 atcgcgagtt gggcatcgag gtgttggcgc tggcggtagg gaaagccggt ggcgagcatc
     1561 gcgccgtcca gcgagtcacg tcggccgacc cggattcgac ggtcgttgag gaaggcgccg
     1621 tcgcccttgc tggcggtgaa gatctcgttg cgcagtggat ccaggatcac cgcgtgcacc
     1681 ggttcgcccc ggtcgagctg ggcgatcgac accgcgaact gcggaattcc gcgaaggtag
     1741 ttgtgggtgc cgtccaatgg atcgacggcc caggtgaagc gtgccccggc tttgccggag
     1801 gcacccgatt cctcgccgag gatcgcgtgg tccgggtacg cgcggcgcaa ctcgcgcacg
     1861 atttccccct cggcctggcg atcgacttcc gagacgaaat cgaggtggcg cttttcggtc
     1921 acgacgacgc tgtcgagccg gttcatgtag cgcaggatca cgctgcccgc ggcgcgcgcg
     1981 gcacggatcg caacggtgac ggcaggtcgg gccatggcga ctggatttcc gcaggttgga
     2041 aaaagagcgc gccgcagggg gcggcgcggt gacgatttta gcagcgccgt gccgcccgac
     2101 gttaatcttg cgcgatgaac cagtccaccg cgtcgattcc tccccagccg cagacgatcc
     2161 gattcgtgct gtcgcgcacc tcgcatccgg gcaacatcgg cgcggccgcg cgggcgatcc
     2221 gcacgatggg cttctggcgg ctggtgctgg tggcgccgca acgctatccg gatccgcagg
     2281 cgacggcgat ggcggcgggt gccgatgacg tgctcgcgca cctggccgtg gcggacagcg
     2341 tcacggacgc ggtcggtgat tgcgaacgcg tgtacgggct gtcggcgcgc cgccgcggcg
     2401 tcaatctccc ggaactcggt ccgcgcgcgg cggtggcgga aatcgcgccg ctcgtgcggc
     2461 agggaaggga agtggccttg ctgttcggca acgagcgttc gggactcgac aacgaggagc
     2521 tgtcggtgtg ccacgcgatg gtgcggatac cgagtgtcga ggagttttcc tcgctcaacc
     2581 tcgcgcaggc cgtgcaactc atggcctggg aattgcggat ggcgatgctc ggcgaggtac
     2641 ccgcggcgcc cccggcgcac gacgaccccg ccaccgccga ccagatggag cgctttttcg
     2701 agcatctgtt cgccacgctg cacgcgatcg acttccacaa gggccgctcg gccaccacca
     2761 tcgagcagcg gctgcgccgg ctgttccacc gcgccatgcc cgacgcacgc gagttgcgta
     2821 tcctgcacgg cgtgctcgcc gatgcggagc gcatggcgcg gctggcgaaa ggcgagatcc
     2881 ccccgccgct gcgaggcgac ccccttcgcc cgcgaagggg gtgaaacgag ggggatggct
     2941 tttgcgcttg ttacccgccg ccgaggcccg cttcgtcctc ggcttcgctc cttgccagcg
     3001 gcgtgacaag caccttgtcg atccgaacgc catcgaggtc gacgacctcg aagctccatt
     3061 gctgccagac ggtgttttcg cccaccgcgg gaatgtggcc gagcgcgtcg atcagcatgc
     3121 cggcgagggt gtaatagtcg ccgtccttct cgttcggcag ctcgtcgatc tgcagcagtt
     3181 cgcgcaggtc gtcggtggac agcgagccgt cgaccagcca gcttccgtcg tcgcgcggca
     3241 ccgcggaacc gccgtgcgag gccttgccgt ccgcgtgggt cgcgcgtccc accactgcgt
     3301 tcaggaggtc attgacggtg accagcccct cgatgtcgcc gtactcgtcg accaccagcg
     3361 cgaacggcat gcctgcgtcg cggaattcgt cgaggatgcc gagtgcgcgc gcggtggcgg
     3421 gcacgaacaa cggcttggcc agcttgcgga acaggtcggg cttggccggc gccatgccct
     3481 tgcgcgggtc ctgcgacatc gcctggagca ggctcttgac ctcgagcacg cccagcactt
     3541 cgtcttcgtc cgcacggtac accgggtaac gcgagtaggg cgtcgcgtgc atcacggcga
     3601 ggttttcctc gagcggtgcg gcggcatcga gccacgcgat gcgtgggcgc ggcgtcatca
     3661 ggctgtcgac ggtgcgatcg ccgagccgca gcacccggtt gaccatgttg cgctcgtcgg
     3721 gatcgagcac gccctgctcg gcgccttcgg cgaccagcat ccggatttct tcctcgctca
     3781 ccttctcgcg gcggaccttg tgcatcccga gcaggcgcag caccaattcc gacagggcgg
     3841 tcaacaccca cacgaacggc gtcagcagcc acgacagcac ctgcatcggg aacgccacga
     3901 cctgtgcgat gcgctcgggg gcgatcaatg ccacgcgctt gggcaccagc tcccccaccg
     3961 tgatgttgat ggtggtcacg aggatgaacc cgagcaccaa cccgatcacc agcgaggcgt
     4021 tcgcggcggc gggcatgccg aggtcgatga gccattgacg gatgcgatcg ctgacggcgg
     4081 cgccggcggc ggcaccggtg atcaaggtga tcaaggtgat cccgacctgc accgtggaca
     4141 ggaagcggtc ggggcgctgg gcgagccgca gcgccacctg tgcgcgggta ctcgtccgcg
     4201 ccatgtgccg aagacggctt ttgcgcgcgg cgatcagtgc aatttcggcc agcgcgaaga
     4261 acgcgttgca cagcgcaagc agcaatacca ggccgagttc ggtcagcatc ggggccgtgc
     4321 gcgagggtcc atcggggaag tttagcaagc gtcgcggcgc taggggccgg cgcgctgctc
     4381 gtgtaccatt tacgcgatct tgcacggatg aaccgccatg ttttcgctgc agacgatgtt
     4441 cggcaagggc gatcgtttct acgatctcct ggaggccagt gccggcgccg cccgcgatgg
     4501 cgccaaggcg ctccagggat tgctcgaggc gcacggcgaa ccgatgctgc tagacggttt
     4561 ccgggcggcg cggcgccgcg agaaggaact ggcgaccaac atcagcgaag agctgatcaa
     4621 cacgttcgtc acggcgctcg atcgcgagga catcgaggcg atgtcgtccg cgctctacag
     4681 gattcccaag acgatcgaga agttcgccga gcgctacgtg atcgtcgccg accgcgtcga
     4741 gggaatcgac ttcgcttcgc gcgccagcct gctgatgagc gcgaccgaag gcgtgttcga
     4801 gatggtccgc gagctgcgca acggcctcag catcgaccgg atgcgcaagc tgcaggaccg
     4861 cctgcaggca gtcgaagccg aggccgaccg catgctgctg gaaccgtacc gcgccctgta
     4921 cctgggcagc aacgatccga tccgcatcat gctcgcgaag gacctgttcg agctgctgga
     4981 aaaagccgtc gaccgctgcc gcgacgtcgg caacgtgatc tattcgatcg tgttgaagaa
     5041 ttcatgagac ggggaccggg gaccggggac cgggggccgg aaaagcgcga agtcgaccag
     5101 ccccttccgc aggaacgggg tcgccgcgat gcggcggggg gatgtacttc gccgtggaaa
     5161 gccatcccgc tcaatccccc ttcctgcgga agggggagga agagcaggag gcacgcgcga
     5221 tattcccagg tccccagccc ccggtccccg gtccccgcat gagcctcggc ctccttaccg
     5281 ccgtcatcgt gatcgcgttg gtgttcacct acatcaacgg cttccacgac accgccaatt
     5341 cgatcgcgac ggtggtcgcg accaaggtgc tgtcgccagg gc
//
LOCUS       DASSNH010000202         5702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_133747, whole genome shotgun sequence.
ACCESSION   DASSNH010000202 DASSNH010000000
VERSION     DASSNH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133747"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFK00_07400"
     CDS             complement(<1..615)
                     /locus_tag="VFK00_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7592812.1"
                     /translation="MLKVDVHSHILPPDWPDLAGKYGDPRFPVMVNAGGHHRIYKDRK
                     FFREVWQNAFDPEFRRDECAKLGVHVQVISTVPVLFSYWAKPNQARELHRFLNEDTAE
                     TCRRYPRNYAGIGTVPLQSPRLAIEEMEHCVEQLGLQGVQIGSHVNNWNLDAPELFPF
                     FEAAADLGAAILIHPWDMMGKETMPKYWLPWLVGMPAEQSRAACC"
     gene            complement(713..1777)
                     /locus_tag="VFK00_07405"
     CDS             complement(713..1777)
                     /locus_tag="VFK00_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HET7592813.1"
                     /translation="MHTWIRQAIATLEAEAARSADTHLIRLDLPAFPGIPLYFKDESV
                     HPTGSLKHRLGRSLFLYAICNGWLERDRPVIEASSGSTAISEAYFARLLALPFIAVMP
                     RHTSRQKIAQIEFFGGMCHLIDGTDVYGAAQQLAEDRGGHYMDQFTHAERATDWRGNN
                     NIAESIFRQMQFEPDPVPRWIVVSAGTGGTSATLGRYIRYGCAHREQRTMLAVVDPEN
                     SVFLDYYRTGDPTLTLERGSRIEGIGRPRVEPSFLPHVIDRMIRVPDADSMAALRVLE
                     KLLGRRCGGSTGTNFVGALQLMGEMKAAGETGPVVTLICDGGDRYAGSYYDDAWLASQ
                     QIDITEPRARLARLAHTGQA"
     gene            1985..2632
                     /locus_tag="VFK00_07410"
     CDS             1985..2632
                     /locus_tag="VFK00_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592814.1"
                     /translation="MIRVVLVDDHELVRSGLRLILAEQPDIEVVGEASNAEDGLRLVR
                     ASKPDVALIDVHMPGMSGIELTERVQRTDLPTRIVILTVVQDAQFPRRLLQSGALGYL
                     TKGCPAQELLEAVRAVAAGRRYLDVTVAREMALAAVDGDGSPLESLSSRELEVALMLA
                     RGLAVNEVANRLHLSAKTISTYKQRLFEKLGVAHTIALAHVLSAHGLLGEPQAQI"
     gene            complement(2768..5698)
                     /locus_tag="VFK00_07415"
     CDS             complement(2768..5698)
                     /locus_tag="VFK00_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET7592815.1"
                     /translation="MKRMLINATQREELRVAIVDGQSLYDLDLEIPSREQKKSNIYKA
                     RITRVEPSLEACFVDYGADRHGFLPLKEIAREYFASGVSPGKAGIRELLQEGQQLIVQ
                     VEKEERGNKGAALTTYISLAGRYMVLMPNSPNAGGVSRRIEGEDRQALKEALQHITVP
                     EEMGLIVRTAGVGRDAEELQWDLDYLLQLWKAISEAAAARSAPFLIYQESKLFIRALR
                     DYLRPDIGEILIDEPALYEDARQFMQQVMPNSLRKLKLYDDATPLFTRFQIETQIESA
                     YDRYVRLPSGGSIVIDQTEALTAIDINSSRATKGSDIEDTAFHTNCEAAVEVARQLRI
                     RDLGGLVVIDFIDMESSRHQREVEDKLRDALKVDRARVQLGRISRFGLMELSRQRLRP
                     SLEESTQIVCPRCDGHGRIRGIESLSLSALRLIEEHAMKENTGQVLVQAPTRVANFLL
                     NEKRAGVVEIELRHKVHVVIVADTNLETPRLEITRIRESEMGEHAKPSYERMTDVVAA
                     PQPRMGVVIHGEEPAVSGIQRATPMPQREEPASAAEAATAVPATAQAASGGLLGRLFG
                     WFRGAPEREPAALESPRPARASQGSNSSGSRRDRSPDRRQESASQHAGGRRGGRDNGG
                     QRSRPSQQRSSQAKSPRSRDDDASRQAQQKPAAQASAHAGNNEQAPRGSARKSARRDT
                     TTPESKEAALTETAAAPATDAAAVEANPAAAPVDAAANGGTAAGSGAKRRRGRRGGRR
                     RKHPATAEAGAATDAPHDIDDEDADRSARASRVAAKPAQPEQSPSSSPSRDANRRPPA
                     AANAPESSATEPPPAKPANGHAVAGSSPAAAPPAPKAPQESASQPAANGSRPDAAPPG
                     QPAPPKTTPTAIAQPAAPHTPAVPTPQPAPGSAPVVVAPEKVAAPAVSKPEPITPSLF
                     AAPPTTNPDPRPQPRHPAIPMPRPLSGAIHPVLAPQVLPQTPPPAAKPSGDTAPSAAD
                     KPQE"
BASE COUNT          801 a         1833 c         2051 g         1017 t
ORIGIN      
        1 gcagcaggcg gcgcgcgact gctcggccgg catcccgacg agccagggca gccagtactt
       61 cggcatggtt tccttgccca tcatgtccca tggatggatc aggatcgcgg cgccgaggtc
      121 cgccgccgct tcgaagaacg gaaacagctc gggcgcgtcg agattccagt tgttgacgtg
      181 cgacccgatc tgcacgccct gcaggccgag ctgctcgacg caatgctcca tttcctcgat
      241 cgcgaggcgc ggcgactgca ggggcacggt gccgatgccc gcatagttgc gcggataccg
      301 gcggcaggtc tcggcggtgt cctcgttgag gaaacggtgc agctcgcgcg cctggttggg
      361 cttggcccag tacgaaaaca gcacgggaac ggtcgagatc acctgcacgt ggacgccgag
      421 cttcgcgcat tcgtcccggc ggaactccgg atcgaaggcg ttttgccaga cctcgcggaa
      481 gaacttgcgg tccttgtaga tccggtggtg gccgcccgcg ttcaccatca ccggaaagcg
      541 cgggtcgccg tattttccgg cgagatccgg ccaatccggc ggcaggatgt gcgaatggac
      601 gtcgaccttc agcatgctgc cagtataggc gacgggcggg gcgccacagg tacgcactga
      661 acgggcaggt tgcgggtgcg gcgctcctgc ccgttcgtcg gcgatgcgtg catcaggcct
      721 ggccggtatg tgccaggcgt gcgagcctcg cgcgcggctc cgtgatatcg atctgttggg
      781 aagccagcca cgcgtcgtcg taatacgacc ccgcgtagcg gtcgccgccg tcgcaaatca
      841 acgtgaccac cggcccggtt tcgccggcgg ctttcatctc gcccatgagt tgcagcgcgc
      901 ccacgaagtt ggtgccggtg gaaccgccac agcgccgtcc gagcagcttc tccagcaccc
      961 gcagcgcggc catgctgtcg gcgtcgggga cgcggatcat gcggtcgatc acgtggggca
     1021 ggaacgacgg ctcgacccgc gggcggccga tgccttcgat gcgcgatccg cgctccagcg
     1081 tgagcgtcgg gtcgccggtc cggtagtaat cgaggaacac cgagttttcc ggatcgacca
     1141 ccgccagcat cgtgcgctgc tcgcggtgcg cgcagccgta gcggatgtag cggccgagcg
     1201 tcgccgaggt tcccccggtg ccggcgctga ccacgatcca gcgcggcacg ggatcgggtt
     1261 cgaactgcat ctgtcggaag atcgattcgg cgatgttgtt gttgccgcgc cagtcggtcg
     1321 cgcgttcggc atgcgtgaac tggtccatgt agtgtccgcc gcgatcctcg gcgagttgct
     1381 gcgcggcgcc gtacacgtcg gtgccgtcga tcaggtggca catcccgccg aagaactcga
     1441 tctgcgcgat cttttgcctg gaggtgtgcc gcggcatcac cgcgatgaac ggcagcgcca
     1501 gcaggcgcgc gaagtaggcc tccgaaatcg cggtactgcc gctggatgct tcgatcaccg
     1561 ggcgatcgcg ttcgagccag ccgttgcaga tcgcgtacag gaacaacgaa cgcccgagcc
     1621 gatgcttgag gctgccggtc gggtgcaccg attcgtcctt gaagtacagc ggaatgccgg
     1681 ggaacgccgg caggtcgagg cggatcaggt gggtgtcggc cgagcgcgcg gcttcggcct
     1741 cgagcgtggc gatcgcctgc cggatccatg tgtgcatccg cgcattttag tcggatcgtc
     1801 gcaatgccat gaccatcgtc cacgagtggt tctgcttgta catcatgagc gccgaggcgt
     1861 ttccgatcgg cgccgcgctc ggcaacagtc gccacggaca gcgtgccgcg ggcgggttgc
     1921 aatcccggaa tatggggttt agggtggttg gagacccgca tcggcacacc caggggggaa
     1981 gcgcatgatc cgcgtggtgt tggtggacga ccatgagctg gtgcgctccg gattgcgcct
     2041 gatcctggcc gaacagcctg atatcgaagt ggtgggcgag gccagcaacg ccgaagacgg
     2101 tttgcgcctg gtccgtgctt ccaagcctga tgtcgcgttg atcgacgtgc acatgcccgg
     2161 catgagcggc atcgaactga ccgaacgcgt gcagcgcacc gatcttccga cccgcatcgt
     2221 gatcctcacc gtggtccagg acgcgcagtt cccgcgccgc ctgctgcagt ccggagcgct
     2281 cggctacctg accaaggggt gcccggccca ggaattgctc gaggcggtgc gagccgtcgc
     2341 cgcggggcgc cgctacctcg acgtgaccgt tgcccgtgaa atggcgctcg cggcggtcga
     2401 tggcgacggg tcgccgctgg aatcactgtc ttcgcgggag ctcgaggtgg cgctgatgct
     2461 ggcgcgcggg ctcgcggtca acgaggtcgc gaaccggctg cacctcagcg ccaagaccat
     2521 ttcgacctac aagcagcgcc tgttcgagaa gctcggcgtc gcccacacga tcgcgctcgc
     2581 gcacgtgctg tcggcgcatg ggttgctcgg ggagccgcaa gcgcagattt gagcgcaggt
     2641 tgcgtcgccc agcgggtgcg caattcggtc gcgtagatgg agcaagcgct tcgcgcaggc
     2701 ccgctcctgc aacgcagggc cgacgatatc ggccctgcgg cggacgcgca cgccggactc
     2761 cgcgagctta ttcctgtggc ttgtcggcgg ccgaaggggc ggtgtctccg ctcggcttgg
     2821 cggctggcgg tggcgtctgg ggcaggacct gcggtgccag caccggatgg atagcgccgg
     2881 acagcggccg cggcatcggg attgccgggt gccgcggctg tgggcgcggg tcgggattcg
     2941 tggtcggagg cgccgcgaac agcgatggcg tgatcggctc cggcttcgaa accgcaggcg
     3001 cggcgacctt ctccggagcg acgaccacgg gcgcgctgcc gggcgcgggc tgcggcgtcg
     3061 ggacggcagg tgtgtgtggc gccgcgggct gcgcgatcgc cgtgggcgtg gtcttcggtg
     3121 gcgcgggctg ccccggtggt gcggcatccg ggcgggaacc gttggcggca ggctggctcg
     3181 ccgactcctg tggggccttc ggtgcgggag gcgcagcggc cggactggag ccggctacgg
     3241 catggccgtt cgcgggcttg gcaggcggcg gttcagtcgc gctggactcc ggcgcattcg
     3301 ccgcagccgg cgggcgccgg tttgcgtccc gggacggcga cgaagatggg gattgctcgg
     3361 gctgcgcggg ttttgccgcg actcgcgaag cgcgcgcgct tcgatcggca tcctcgtcat
     3421 cgatatcgtg cggagcatcc gtcgctgcac ctgcttcggc cgtggcggga tgtttgcgcc
     3481 gccggccacc gcggcggccg cggcggcgct tggctccgct gcccgcggca gttccgccgt
     3541 tcgcggccgc gtcgaccgga gccgccgcag gatttgcctc cacggccgct gcatcggtag
     3601 cgggagctgc ggccgtttcg gtgagcgcgg cctctttcga ttccggagtc gtcgtgtccc
     3661 tgcgcgcgga tttgcgtgcg cttccgcgcg gcgcctgctc gttgttgccg gcatgcgcgg
     3721 atgcctgcgc ggccggcttc tgctgcgcct gccgcgacgc atcgtcgtcg cgcgacctcg
     3781 gcgacttcgc ctgcgaggaa cgttgctggg acgggcggct gcgctggccg ccgttgtcgc
     3841 ggccgccgcg gcgcccgccg gcgtgctggg aggccgattc ctggcgtcga tccggcgaac
     3901 ggtcgcggcg gctgcccgac gagttgctgc cctgggacgc gcgcgccggg cgcggcgatt
     3961 ccaatgccgc gggctcgcgt tcgggcgcgc cgcggaacca gccgaacagt cggcccagca
     4021 ggccgccgga ggcggcctgc gcggtcgcgg gaaccgccgt cgccgcttcg gccgcggaag
     4081 ccggctcctc gcgttgcggc atcggtgtcg cgcgctggat gccgctgacc gcaggctcct
     4141 cgccgtggat gacgacgccc atgcgcggct gcggcgcggc gaccacgtcg gtcatgcgtt
     4201 catagctcgg cttggcgtgc tcgcccatct cgctttcgcg gatgcgggtg atttcgagtc
     4261 gcggtgtttc gaggttggtg tcggccacga tcacgacatg caccttgtgg cgcagttcga
     4321 tctcgaccac gccggcgcgc ttttcattga gcaggaagtt cgcgacccgg gtcggcgcct
     4381 ggaccagcac ctggccggtg ttttccttca tcgcgtgttc ttcgatcagc cgcaacgcgg
     4441 acagtgacag cgattcgatg ccccggatcc ggccgtggcc gtcgcagcgc gggcacacga
     4501 tctgggtgga ttcctccagg ctggggcgca agcgctggcg ggaaagttcc atcagcccga
     4561 aacgcgaaat gcgtccgagc tgcacgcggg cgcggtcgac cttcagcgca tcgcggagct
     4621 tgtcctccac ttcgcgctgg tggcgcgagc tttccatgtc gatgaagtcg atcacgacca
     4681 ggccgccgag gtcgcggata cgcagttgcc gcgcgacttc gaccgccgct tcgcagttgg
     4741 tgtggaacgc ggtgtcctcg atgtcgctgc ccttggtggc gcgcgaggaa ttgatgtcga
     4801 tcgcggtcaa cgcttcggtc tggtcgatca cgatcgagcc gcccgacggc agccgcacgt
     4861 agcggtcgta ggcgctctcg atctgggtct cgatctggaa gcgggtgaac agcggggtgg
     4921 cgtcgtcgta cagtttcaac ttgcgcaggc tgttcggcat cacctgctgc atgaactgcc
     4981 tggcgtcctc gtagagggcg ggttcatcga tcaggatctc gccgatgtcg gggcgcaggt
     5041 agtcgcgcag ggcgcggatg aacagtttcg attcctggta gatcaggaag ggcgcggaac
     5101 gcgccgcggc ggcctcggaa atcgccttcc acagttgcag caggtaatcg aggtcccact
     5161 gcagttcctc ggcatcgcga ccgacgcccg cggtgcgcac gatcaggccc atttcctcgg
     5221 gcaccgtgat gtgctgcagg gcttccttca gggcctggcg gtcctcgccc tcgatccgcc
     5281 gcgacacgcc gcccgcgttc ggactgttgg gcatcaacac catgtaacga ccggccagcg
     5341 agatataagt ggtgagcgcg gcacccttgt tgccgcgttc ctccttctcg acctggacga
     5401 tcagttgctg gccttcctgc agcaactcgc ggatgccggc cttgccggga ctgacaccgg
     5461 acgcgaagta ctcgcgggcg atttccttca gtggcaggaa gccgtggcgg tcggcgccgt
     5521 agtcgacgaa gcaggcttcg agcgaaggct cgacccgggt aatccgggcc ttgtagatgt
     5581 tggacttctt ctgttcgcgg gaggggatct cgaggtcgag atcgtagagg gattggccat
     5641 cgacgatggc cacgcgcaac tcctcacgct gagtcgcgtt gatcaacatg cgtttcattg
     5701 tg
//
LOCUS       DASSNH010000203         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1642621, whole genome shotgun sequence.
ACCESSION   DASSNH010000203 DASSNH010000000
VERSION     DASSNH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642621"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFK00_07420"
     CDS             <1..220
                     /locus_tag="VFK00_07420"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HET7592816.1"
                     /translation="KWSGNSFHAEFTCNGNGQDMHGTAEGTMAANGKSYHGTVRMEGD
                     NPHMGGHFVMEGESSGKWLGQTCDKSAG"
     gene            complement(564..1127)
                     /locus_tag="VFK00_07425"
                     /pseudo
     CDS             complement(564..1127)
                     /locus_tag="VFK00_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806208.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1403..2779
                     /locus_tag="VFK00_07430"
     CDS             1403..2779
                     /locus_tag="VFK00_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7592817.1"
                     /translation="MHVSKRKVGLVTVTALVLGNMIGSGVFLLPASLAPYGRYSLVGW
                     TVSAAGALLLAGVFYRLAKRAPRAGGPYAYSREAFGDCVGFLVAWVYWIALVGGNATI
                     AVAFASYMSAFIPALGAHPLYGALTAMAAVWVLIAVNIAGIRSAGAMQVVTTILKILP
                     LLAVALFGLMHFDPQLLAPGPHAGSALGAINVCVAATLFAFIGVECATIPAGHVRDPE
                     KTIPRATLLGTAIAAALYIACTAVVMGLLPASELAKSQAPFADAARLLWGGWASGLIA
                     GAAAISCFGALNGWTLIAGQFPQAVAKDGLFPGIFARESARGTPVPALLVAGVINSLM
                     VLANYSRGMVAMFTFMVLLTTLGNVVCYLFCTMADVVLAQRDGRRAPSRNLLLAFAAF
                     GFCLWAVIGAGKGAVFWNFVLLALGIPLYAWQARRGTAAAVPPADVALPLPPVPEVRS
                     VGMARRAD"
     gene            complement(2801..3196)
                     /locus_tag="VFK00_07435"
     CDS             complement(2801..3196)
                     /locus_tag="VFK00_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET7592818.1"
                     /translation="MSDQTAIRIDAWLWAARFFKTRSLAKQAVEHGRVTIAGASCKPS
                     HIVRIGDSLKITRGEETFEVEVLALSGKRGSAARAQTLYSESEASIRAREEARERRRA
                     ERGGYQPPAKRPDKRARRLIRALGDIDSR"
     gene            complement(3259..>3808)
                     /locus_tag="VFK00_07440"
     CDS             complement(3259..>3808)
                     /locus_tag="VFK00_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460950.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7592819.1"
                     /translation="AAAGSPQLEAFKAKDIEVLLMFDRVDEWIMGQFGEYEGKPFRNV
                     AKGELPLDEADKKKQEEVAKEAEPLVAKLKELLGDRVSEVKVSARLTDSPSCLALGDY
                     DLAPHLARLLREAGQEVPDSRPTLEINPAHPLVQRVQSETDEARAKDLALLLLEQAEI
                     TAGAQLPDPAAFVQRMNRMLAG"
BASE COUNT          547 a         1295 c         1304 g          662 t
ORIGIN      
        1 gaagtggtcg gggaacagct tccatgccga gttcacctgc aacggcaacg gacaggacat
       61 gcatggaacg gccgagggaa cgatggcggc gaacggcaag tcctaccatg gcaccgtgcg
      121 catggaaggc gacaatccgc acatgggcgg ccacttcgtg atggaagggg agtcctcggg
      181 caagtggctg ggccagacct gcgacaagag tgccgggtag tcctgcacgc gcaggaatac
      241 cgaaatcagg atcagtcgag gatgtcgtcg ccggcgcgtt ccatggccgc tccggcctcg
      301 atcgcgctgg ttgccgcctc gcggtgccgc ggctgatccg tggacacgga gatccgcggg
      361 cccggtgcgc cgctcgcgtc gggaaccaat gtcgggcgga tcgccatgct ggtcgccgtt
      421 ccgacgatca cgattcctgg cggcggctcc gcgcttcttg cacgtgccgt gcggccgcac
      481 gcaacgcctg catccggttg gatgcatacc gtggccggcg acggcggacg gctcgccggc
      541 ggcacgaaac cgggcaccac gtgctccgcg tcgggcatcg cgatctcggg gatcgcagcc
      601 cactcttccg gtgaaatgtc cgcgggaatt cccggcgccg gcattggcgg cgtcgggcgc
      661 ccgggcatgc gtccggcctg cacaagttcg cggatcgcat ggaacacctc tcggcgcccg
      721 aacggcttct tgacgaaccc gtcggcgccg aagcgctgca cgtagaactg ctccgtcgcc
      781 tgctggttgc cgctcatcat gacgatgggc gtggccttcg tgcgcggatc gtgccgcaac
      841 gcccgcaaca ccgcgaatcc cgacatcccc ggcagtacga tgtccaggaa gatcaggtcg
      901 ggggattgcg accgcgccac ctcgatcgcg gcctcgccat cgccggcttt caggacctcg
      961 taaccgtcct gcaccagcat cttgccgagc acggcgcgaa tcgtcgccga gtcgtcgacc
     1021 accagcagcc gggcgccgag cgtggtgcgg acgcgcgcgt ccacccggcg ttcgccgccg
     1081 gccgtttcgg tgttcgcccg tcgcttgaag atgtcgagta tcgccatcgc cccgcccctg
     1141 aagctccgcg ggccagtttc gacggcacaa atgagcggcg caagctccgg gcagcacgaa
     1201 tcctacatgg atcacgaatt taccccgcct ccttggcatg gacttcaagt cgagcccaca
     1261 acccattgaa ttcgcggtac tcggcccgag cgggggaccg gagtgcaccg ctgcggatcg
     1321 ccgcgcgatc gccgccgttc gccctgcatg cacgcgtaca atcccggaac ggtccagccg
     1381 acggcaccgc acgggggctc cgatgcacgt ctcgaaacgc aaggtcggcc tggtcaccgt
     1441 caccgcgctg gtgctcggga acatgatcgg atcgggcgtg ttcctgctgc cggcctcact
     1501 cgcgccgtac ggccgctaca gcctggtcgg ctggacggtc agcgccgcgg gggcactgtt
     1561 gctggccggc gtgttctacc gcctggcgaa gcgcgcaccg cgcgccggcg gcccgtatgc
     1621 ctatagccgc gaagccttcg gcgattgcgt cggcttcctc gtggcgtggg tgtactggat
     1681 cgcgctggtg ggcggcaacg cgacgatcgc cgtcgcattc gcgagctaca tgtccgcgtt
     1741 catcccagcg ctcggcgcgc acccgttgta tggcgcactg accgcaatgg ccgcggtatg
     1801 ggtgctgatc gcggtcaaca tcgccgggat ccgcagcgcc ggcgcgatgc aggtggtcac
     1861 gacgatactg aagatcctgc cgctgctggc cgtggcgttg ttcgggctca tgcacttcga
     1921 cccgcagctg ctggcgccgg gccctcacgc gggttcggcg ttgggcgcga tcaacgtgtg
     1981 cgtcgcggcc acgttgttcg cgttcatcgg cgtggaatgc gcgacgatcc cggccggcca
     2041 cgtgcgcgat ccggaaaaga ccatcccgcg cgccacgttg ctcggcaccg ccatcgcggc
     2101 ggcgctctac atcgcgtgca cggccgtggt gatgggcctg ctgcccgcat cggaactcgc
     2161 gaagtcgcag gcgccgttcg ccgacgcggc gcgcctgctg tggggcggat gggcgagcgg
     2221 gctgatcgcg ggcgccgcgg cgatctcctg cttcggtgcc ttgaacggct ggacgctgat
     2281 cgccggacaa tttccgcagg cggtggcgaa ggatggattg ttcccgggga ttttcgcgcg
     2341 ggaatccgcg cgcggcacgc cggtgcccgc gctgctggtg gcgggcgtca tcaacagcct
     2401 gatggtactc gccaactaca gccgcggcat ggtcgcgatg ttcaccttca tggtgctgct
     2461 gacgacactc ggcaacgtcg tctgctacct gttctgcacg atggccgacg tggtgcttgc
     2521 gcaacgcgac gggcgccgtg cgccgtcgcg caacttgttg ctggcgttcg ccgcgttcgg
     2581 gttctgcctg tgggccgtga tcggcgccgg caagggcgcg gtgttctgga atttcgtgct
     2641 gctggcgctc ggcattccgc tgtacgcatg gcaggcaagg cgcggcacgg cagccgccgt
     2701 gccgccagcg gacgtggcac tcccgctgcc cccggtcccg gaggtccgca gcgtgggcat
     2761 ggctcggcgc gccgattgaa cggcggaggg ggggggcgcc ctaccggctg tcgatgtcgc
     2821 cgagggcgcg gatcaggcgc cgtgcgcgct tgtcgggccg cttcgctggc ggctggtagc
     2881 cgccgcgttc ggcgcggcgg cgttcgcgcg cttcctcgcg tgcacggata gaagcttcgg
     2941 attccgagta caacgtttgc gcacgcgcgg ccgaaccgcg cttgccggac aacgccagca
     3001 cttcgacttc gaaggtttcc tcgccgcgcg tgatcttgag cgaatcaccg atacgtacga
     3061 tatgcgacgg cttgcaggac gcccccgcga tcgtcacccg cccgtgttcg accgcctgct
     3121 tggcaagcga ccgggtcttg aagaaccgcg cggcccagag ccacgcgtcg atgcggattg
     3181 cagtctgatc gctcattgcc gggctcggcg aagctcgttg tgccttggca agtcctgttg
     3241 aatcccgacg atcggccgtt accccgccag catccggttc atccgctgca cgaatgccgc
     3301 ggggtcgggc aattgcgcgc cggcggtgat ttcggcctgc tcgagcaaca gcagcgcaag
     3361 atccttggcc ctggcctcgt cggtttccga ctggacgcgc tggaccagcg ggtgcgcggg
     3421 attgatttcc agcgtcggcc tggagtccgg aacctcctgg cccgcctcgc ggagcagccg
     3481 cgcgagatgc ggcgcgaggt cgtagtcgcc gagcgcgagg cacgagggcg aatcggtcag
     3541 gcgggcggaa accttcacct cggagacgcg atcgcccagc agttccttga gcttcgcgac
     3601 cagcggctcg gcttccttcg cgacctcttc ctgcttcttc ttgtcggcct cgtccagcgg
     3661 cagctcgccc ttggcgacgt tccggaacgg cttgccttcg tattcgccga actggcccat
     3721 gatccattcg tcgacgcgat cgaacatcag cagcacctcg atgtccttcg ccttgaaggc
     3781 ctcgagctgc gggctgccgg ccgcggcc
//
LOCUS       DASSNH010000204         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_395406, whole genome shotgun sequence.
ACCESSION   DASSNH010000204 DASSNH010000000
VERSION     DASSNH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395406"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VFK00_07445"
     CDS             complement(<1..1394)
                     /locus_tag="VFK00_07445"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET7592820.1"
                     /translation="MDQSRLPGFYKLSVPERVNLVRERGLLSREDFQVLASGAHTLTV
                     ARADKMIENVIGVMGLPLGLGLNFLVNGKDHVVPLVVEEPSIVAALSSAAKLVRAAGG
                     FTTRSTEPILIGQVQIVDIDNSATAQAALLQRKQEILNLANSLHPKMVARGGGAKDIE
                     VRIHPAADGRRDMVVLHLLVNTCDAMGANMVNTMCEGVAPLVEGITGGKVFLRILSNL
                     TDRALVWAKAVIPTELLAGKGYDGEQVRDGIILANDLAVCDPYRAATHNKGIMNGVDA
                     AAIATGNDWRALEAAAHAYAARGEHYTSLTRWYKDDQGNLVGTIEMPMKVGTVGGNLQ
                     SNATVALNLRLANVATARELAEIMGAVGLAQNFSALRALVTDGIQQGHMTLHARSVAV
                     SAGATPEIFETVVERLLDSGEIKVWKAREIIAEVKNQVQPEAKTVAHGFEPVGEAEYA
                     TGYGKAILLGEHAVV"
     gene            complement(1414..>2247)
                     /locus_tag="VFK00_07450"
     CDS             complement(1414..>2247)
                     /locus_tag="VFK00_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592821.1"
                     /translation="GGARWIGDAPPELEWVATLLARVPQAQALPPCRVELDSDAFYLD
                     HGGTRGKLGLGSSAALTVALLGALHVHAHADPPAIDDAIETHRAIQHGHGSGIDIAAS
                     LAGGLSRFRLDGDAVRVAPIALPEDLHWVCVYSGRPASTRAMLATVAAWREREPSAFA
                     QQTHELATISSRGIDALTANDAAAFLSSIHDYATALARFGVAAGADIASREHRAIAAI
                     AEACGCVYKSCGAGGGDIGVTFAVEDTRLREFSERAARAGFPTIGLKADPKGLEVVSN
                     V"
BASE COUNT          357 a          747 c          751 g          392 t
ORIGIN      
        1 accaccgcgt gctcgcccag caggatggcc ttgccgtagc cggtcgcgta ctcggcttcc
       61 ccgaccggct cgaaaccgtg tgcgacggtc ttggcttcgg gctgcacctg gttcttgacc
      121 tcggcgatga tttcgcgcgc cttccacacc ttgatctcgc cggaatcaag caggcgctcg
      181 accaccgttt cgaaaatttc cggcgtcgca cccgcgctca ccgcgaccga acgcgcatgc
      241 aaggtcatgt ggccttgctg gatgccgtcg gtgaccagcg cgcgcagcgc ggagaagttc
      301 tgggccaaac ccacggcgcc catgatctcc gcgagttcgc gcgcggtcgc gacgttggcg
      361 agccgcaggt ttagggcgac ggtggcgttc gattgcaggt tgccgccgac cgtgccgacc
      421 ttcatcggca tctcgatggt gccgaccagg ttgccctggt cgtccttgta ccagcgtgtc
      481 agggaagtgt agtgctcacc gcgcgccgcg taggcgtgcg ccgcggcttc cagcgcgcgc
      541 caatcgttgc cggtcgcgat ggcggcggca tcgacgccgt tcatgatgcc cttgttgtgg
      601 gtggccgcgc gatacggatc gcacaccgca agatcgttcg cgaggatgat gccgtcgcgt
      661 acctgttcgc cgtcataacc cttgcccgcg agcagttccg tcgggatcac ggccttggcc
      721 cacaccagcg cgcggtcggt gagattggaa agaatgcgca ggaacacctt gccgccggtg
      781 atgccttcga ccaatggcgc cacgccttcg cacatggtgt tgaccatgtt ggcgcccatc
      841 gcgtcgcagg tattcaccag caggtgcagc accaccatgt cgcggcgccc gtcggcggcg
      901 ggatggatgc gcacctcgat gtccttggcg ccgccgccgc gcgcgaccat cttcgggtgg
      961 aggctgttgg cgaggttcag gatttcctgc ttgcgctgca gcaacgcggc ctgtgcggtg
     1021 gccgagttgt cgatgtcgac gatctgcacc tggccgatca ggatcggttc ggtcgaacgg
     1081 gtggtgaagc cgcccgccgc gcggaccagc ttcgcggcgg atgacagcgc cgccacgatc
     1141 gagggctcct cgaccaccag cggcaccacg tggtccttgc cgttgacgag gaagttgagg
     1201 ccgagcccga gcggcaaacc catcacgccg atcacgttct cgatcatctt gtcggcgcgc
     1261 gcgacggtga gcgtgtgcgc gccgctcgcc agaacctgga agtcctcgcg cgaaagcagg
     1321 ccgcgctcgc gcaccaggtt cacacgttcc ggcaccgaca acttgtagaa acccggcagc
     1381 ctcgattggt ccattttgat tccttgcaaa gcctcaaacg ttcgaaacga cctccagacc
     1441 cttggggtcc gctttcagtc cgattgtggg aaatccggcg cgcgccgcgc gctcagaaaa
     1501 ctcccgcaac cgcgtatctt ccacggcgaa cgtgacgcct atgtcaccgc cgcccgcgcc
     1561 gcagctcttg tagacgcatc cgcaagcttc ggcaatcgcg gcgatcgccc ggtgttcgcg
     1621 gctggcgatg tccgcgcccg cggccacgcc gaagcgcgcc aaggctgtcg cgtaatcatg
     1681 gatggaagac aggaaggcgg cggcatcgtt ggcggtcagg gcgtcgatac cgcgtgaaga
     1741 aattgtagcg agttcgtgcg tttgctgcgc aaaggcggac ggctcgcgtt cacgccaggc
     1801 ggcgaccgtg gcgagcatcg cgcgggtcga cgccggccgg ccgctgtaga cgcaaaccca
     1861 gtgaagatcc tccggcagcg cgatgggtgc gacccggacc gcatcgccgt ccagccggaa
     1921 gcgcgagagt ccgcctgcca gcgacgccgc gatgtcgatg ccgctgccat ggccatgctg
     1981 gattgcgcgg tgtgtttcga tggcgtcgtc gatcgccggt gggtcggcat gggcgtgtac
     2041 gtgcaacgcc ccgagcagcg cgacggtgag cgccgcactg gaaccgaggc ccagtttccc
     2101 gcgggtgccc ccgtgatcga gatagaacgc gtcgctgtcg agctcgaccc ggcaaggtgg
     2161 cagggcttgc gcttggggaa cacgcgcgag cagcgtggcg acccattcga gctcgggagg
     2221 tgcgtcaccg atccagcggg cgccgcc
//
LOCUS       DASSNH010000205         6509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_259854, whole genome shotgun sequence.
ACCESSION   DASSNH010000205 DASSNH010000000
VERSION     DASSNH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259854"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..449
                     /locus_tag="VFK00_07455"
     CDS             24..449
                     /locus_tag="VFK00_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7592822.1"
                     /translation="MRHPLHDDFRQLLDQFFETGESGDQSDVVTSQWAPRVDIREEDK
                     RFVIEADIPGVDPKDIDINMDKGVLSIRGERKSESKTGNGKFTRVERSHGVFYRRFAL
                     PDSANPDGISATGRHGVLEIEIPKKPETTPRKIDIKTGE"
     gene            548..1468
                     /locus_tag="VFK00_07460"
     CDS             548..1468
                     /locus_tag="VFK00_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7592823.1"
                     /translation="MEFQDYYDTLGVKSGASEDEIKSAYRKLARKYHPDKNKEAGAED
                     KFKAISEAYEVLHDKQKRAAYDNLRAGGYRGGDSFRPPPGWQQQAPGGFEFRDFGGGG
                     DAGGFSDFFESLFGGRAAGAGARAQRGPLRGNDVRAAVEIDLRTAYAGGKQRLGLRDA
                     SGERTLEVNIPAGILPGKVIRLAGQGEPGYNGGPAGDLLLEVQVRDDPQFKLHGRDVE
                     VEVPVSPWEAALGAKVPVTTLGGTVELRIPAGSRSGRKLRLKGRGMPAHTPGDQIVAL
                     SIQIPPAEDAKAKAAYHAFQKAFSGFDPRA"
     gene            complement(1529..1894)
                     /locus_tag="VFK00_07465"
     CDS             complement(1529..1894)
                     /locus_tag="VFK00_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592824.1"
                     /translation="MARILVVDDSPSQLEGLKRLVEKLGHQTITAVDGAAAVEAAKRE
                     KPDLVLMDVVMPNLNGFQATRAISKDATTAHIPVVLVTTKDQDTDKVWGMRQGAKAYV
                     TKPVNEAELTRTLNDHLPG"
     gene            complement(2030..3037)
                     /locus_tag="VFK00_07470"
     CDS             complement(2030..3037)
                     /locus_tag="VFK00_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HET7592825.1"
                     /translation="MSNRASFRAFRIRSDDTGYRAGIETMRVDDLSPGEVVVKTAYSS
                     VNYKDALAGTGRGKILRRFPLNGGIDVAGHVVESRDARFKEGDAVLCTGCGLSETRDG
                     GYAEYARLEAAWTVPLPRGLGLRESMILGTAGFTAALALYRMRDNRQSPALGPIAITG
                     ATGGVGMLAVDIFSRAGFAVHAISGKPDRADFLRNLGAREVLDRRGLRLGNHALESAR
                     FGGAVDSVGGKWLAQLLACTSPYGNVASIGLAADSKLETTVMPFIIRGVSLLGIASAG
                     TAREVRDEVWRHLASDWKPAHLDAICTEEIPLEALPANFDAKLGGRSFGRTLVAIAPS
                     A"
     gene            complement(3322..4749)
                     /gene="lpdA"
                     /locus_tag="VFK00_07475"
     CDS             complement(3322..4749)
                     /gene="lpdA"
                     /locus_tag="VFK00_07475"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7592826.1"
                     /translation="MADNKFDVIVIGAGPAGYICAIRAAQLGLKVACVDNFKGKDGKQ
                     ALGGTCLNVGCIPSKALLDSSKQFWGLSHNLPTHGISVENAKVDMATFIGRKDKIVKQ
                     FTGGVGMLFKANKVTPFFGTGKLLKGNKVEVTGEDGNKQALEAVNVIIATGSVPIELP
                     FAKFDGKNIIDNAGALDIDRVPKRLGVIGAGVIGLELGSVWKRMGAEVTILEALPDFL
                     GAADQDIAKAAAREFKKQGLDIHLAAKVSNAQVKKNAVEVTYADKDGEHKITVDKLLV
                     AVGRRAYTQGLLGDGTGVKTDDRGRIEVDEHCWTGADGVWAIGDCVRGAMLAHKGEEE
                     GVMVAELIAGKAGHVDYDVIPWVIYTEPEIAWAGKTEKQCKDEGVPYKTGSFPFAANG
                     RATAMNEPAGLVKVIAHAETDRILGVHMCGPVVSELIAEAVVAMEFKGSSEDLARIVH
                     AHPTLSEVVHEAALSVEKRALNKAN"
     gene            complement(4842..6107)
                     /gene="odhB"
                     /locus_tag="VFK00_07480"
     CDS             complement(4842..6107)
                     /gene="odhB"
                     /locus_tag="VFK00_07480"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET7592827.1"
                     /translation="MSTEVKVPVLPESVSDATIAAWHKKAGDAVKRDENLVDLETDKV
                     VLEVPAPADGVLKEIRHDTGDTVNSQDVIAILEEGAAAEAPQAASEKKEEKQPQPPTA
                     AEAPAKAAEPGTGERGPAKATAGSENLSPAARRVATEENIDTSKVEGTGRGGRVTKED
                     VVNYSKGGPSMPAPAGPRSPVPGPATPGSRPEERVPMTRMRARIAERLMQSKNSIAML
                     TSSNEVNLAKVVAMRKQLGEQFQKTHGIKLGFMSFFVKAACEALKRYPVVNASVDGND
                     IIYHGYQDISIAVATDRGLVTPVLRDAQDMGFADIEKAIADFAERARAGKLTLDDLQG
                     GTFTITNGGTFGSLFSTPIVNPPQSGILGMHTIKERAVVENGQIVAAPMMYIAISYDH
                     RIVDGKDAVLFLVDIKNQLENPHRMLLGL"
     gene            complement(6260..>6509)
                     /locus_tag="VFK00_07485"
     CDS             complement(6260..>6509)
                     /locus_tag="VFK00_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592828.1"
                     /translation="LDKYPAAKEVIWCQEEPQNQGAWFQIRHHLQACTGANRSLHYAG
                     RARSPAPACGHHDTHVAQQHALVQQALAEPVGDDHSAE"
BASE COUNT         1020 a         2135 c         2168 g         1186 t
ORIGIN      
        1 cgaactacca tcgtccctgg ggcatccgcc atcccctgca tgacgacttc cggcagttgc
       61 tggaccagtt tttcgagacg ggtgaatccg gggaccagtc ggacgtggtg accagccagt
      121 gggcaccgcg ggtggacatc cgcgaagagg acaagcgctt cgtgatcgag gcggacatcc
      181 ccggcgtcga tcccaaggac atcgacatca acatggacaa gggcgtgctt tccatccgcg
      241 gcgagcgcaa atcggagagc aagaccggga atggcaagtt cacgcgcgtc gagcggtcgc
      301 acggggtgtt ctaccgccgg ttcgcccttc cggacagcgc caacccggac gggatcagcg
      361 caaccggtcg ccacggcgtt ctggaaatcg aaatccccaa gaagcccgaa accaccccgc
      421 gcaagatcga cataaagacc ggcgagtgag ccggcgcggc gaaagcagcc ttccggcaat
      481 acgccataat cggggtccgc ggcggagcga tccgccgcgg acgatcgtct gagcgcgggg
      541 agggcagatg gagttccagg attactacga caccctcggc gtgaagtccg gcgcgagcga
      601 ggacgagata aagtccgcct atcgcaagct cgcgcgcaag taccacccgg acaagaacaa
      661 ggaagccggg gccgaggaca agttcaaggc catcagcgag gcctatgaag tcctgcacga
      721 caagcagaag cgcgcggcct acgacaacct gcgcgcgggc ggctaccggg gtggcgacag
      781 cttccggccg ccgccgggct ggcagcagca ggcgccgggt ggcttcgaat tccgcgactt
      841 cggtggcggt ggcgacgcgg ggggcttcag tgatttcttc gagtcgcttt tcggtggccg
      901 tgccgcgggt gcgggcgcgc gtgcgcagcg aggaccactg cgcggcaacg acgtgcgcgc
      961 cgccgtcgag atcgaccttc gcaccgccta tgcgggcggc aagcagcggc tcggcctccg
     1021 cgatgcgagc ggcgagcgca cgctcgaggt caacattccg gccggcatcc tgcccggcaa
     1081 ggtgatccgg ctggcagggc agggcgagcc cggatacaac ggcgggccgg ccggcgacct
     1141 gttgctggaa gtgcaggtgc gcgacgaccc gcaattcaag ctgcacggcc gcgacgtcga
     1201 ggtggaagtg ccggtgtcgc cgtgggaggc ggcgctcggc gccaaggtgc cggtgaccac
     1261 actcggcggc accgtcgaat tgcggattcc cgcgggttcg cgcagcggcc gcaagctgcg
     1321 cctgaagggc cgcgggatgc ccgcacacac gccgggtgac cagatcgtgg cgctctcgat
     1381 ccagattccg ccggccgagg acgccaaggc caaggccgcc taccatgcct tccaaaaggc
     1441 gttcagcggg ttcgatccgc gcgcctaggg cgggcatcgg ccccctcgcc aacggaagcg
     1501 ccgacgacgg acagggtccg ccctgcggtt acccgggcag gtggtcgttc aacgtcctgg
     1561 tgagttcggc ctcgttcacg ggcttggtga cgtaggcctt cgcaccctgg cgcatgcccc
     1621 acaccttgtc ggtgtcctgg tccttggtgg tgaccaggac caccgggatg tgtgcggtcg
     1681 ttgcgtcctt ggagatggcg cgggtcgcct ggaagccgtt caagttcggc atcacgacgt
     1741 ccatcaggac caggtcgggc ttttcccgct tggcggcttc caccgcggcg gcgccgtcca
     1801 cggcggtgat ggtctggtgg cccagctttt cgaccagacg cttcaggcct tccaactggg
     1861 acggcgaatc gtcgacgacc agaatgcgtg ccatgcagtg ctccccgcaa cgtttgccgg
     1921 cattgtaggg aaccgggacg cgaagcgcca cgctccgcgt gccgggtcac tatcgacacg
     1981 aagacacaag aaatcagcgc tttgtcgctg gtttgaaatc cggtttcgat caggcggaag
     2041 gagcgatcgc gaccaatgtg cggccgaagg agcgtccccc gagctttgca tcaaagttgg
     2101 cgggcaaggc ctccagcggg atctcttcgg tgcaaatggc gtcgagatgc gcgggcttcc
     2161 agtccgacgc caggtgccgc cagacctcgt cccggacctc acgcgcggtg cccgccgagg
     2221 cgatcccgag caggctgacg ccacggatga tgaagggcat gaccgtggtt tcgagcttgc
     2281 tgtcggcggc gaggccgatg ctggcgacat tgccgtaggg cgacgtgcag gcgagcaact
     2341 gggccagcca tttgccgccc acgctgtcca ccgcgccgcc gaatcgcgct gactcaaggg
     2401 cgtggttgcc gagacgcagg ccgcggcgat ccaggacttc gcgtgctccg agattcctca
     2461 ggaagtccgc acggtcgggc ttgccgctga tcgcgtggac cgcgaaaccc gcacgcgaaa
     2521 aaatgtccac cgccagcatc cccacgccgc cggtcgcgcc ggtgatcgcg atcggaccca
     2581 gggcgggcga ctggcggttg tcgcgcatcc ggtacagcgc cagcgccgcg gtgaacccgg
     2641 cggtgccgag gatcatgctt tcgcgcaggc cgagcccccg cggcagcggc accgtccatg
     2701 cggcctccag ccgggcatat tcggcatagc cgccgtcgcg cgtttccgaa agcccgcaac
     2761 cggtgcaaag cacggcatcg ccttccttga accgggcgtc gcgtgattcg accacgtggc
     2821 ccgcgacgtc gatcccgccg ttcaagggga accggcgcag gatcttgccc ctgcccgtgc
     2881 ccgccagggc gtccttgtag ttcaccgagg agtacgccgt cttgacgacg acctcgcccg
     2941 gcgagaggtc gtccacgcgc atcgtctcga tgccggcgcg gtagccggtg tcgtcgctgc
     3001 ggatgcggaa ggcgcggaag cttgcgcgat tgctcatgct attcccgtca ttccggcgga
     3061 gggccggcac tgtggaggca ggatgccgga acagaacgcc gcaggcggcc tgaagggcga
     3121 gccgccgggt gcggcgagta gtccatcttg cccttggatg ctttgcaaaa tcaaactgga
     3181 ttcccggcct ctgccgggat gacgagcaag agctcgccgc gtcatcgccc agccctgtgc
     3241 tgcactggat gagttcatcc tgaaactgga tcccgtgtca tcggccatcc atggcctgca
     3301 cgggatgacg acatcctgtc gtcaattggc cttgttgagc gcccgctttt ccaccgacaa
     3361 cgcggcttcg tgcacgactt cggacaaggt cgggtgcgcg tggacgatgc gggcgaggtc
     3421 ctcggacgag cccttgaatt ccatcgcgac cacggcttcg gcgatcagtt cggacaccac
     3481 cgggccgcac atgtgcacgc cgagaatgcg atcggtttcc gcgtgcgcga tgaccttcac
     3541 gaggcccgcg ggttcgttca tcgccgttgc gcggccgttg gcggcgaacg ggaacgagcc
     3601 ggtcttgtac ggaacgcctt cgtccttgca ctgcttctcg gtcttgccgg cccacgcgat
     3661 ctcgggctcg gtgtagatca cccacgggat cacgtcgtag tcgacgtgcc ccgccttgcc
     3721 ggcgatcagc tcggcgacca tcacgccttc ttcctcgccc ttgtgcgcga gcatcgcacc
     3781 acgcacgcag tcgccgatcg cccacacgcc gtcggcgcct gtccagcaat gttcgtccac
     3841 ttcgatacgg ccgcggtcgt cggtcttcac accggtgccg tcacccagca ggccctgcgt
     3901 gtacgcgcga cggccgaccg cgaccagcag cttgtccacg gtgatcttgt gctcgccgtc
     3961 cttgtcggcg taggtgacct ccaccgcgtt cttcttgacc tgggcgttgc tcaccttggc
     4021 ggcgaggtgg atgtcgagcc cctgcttctt gaactcgcgc gcagccgctt tcgcgatgtc
     4081 ctggtctgcc gcgccgagga aatcgggcaa cgcttcgagg atcgtgactt ccgcgcccat
     4141 gcgcttccag acggaaccca gttccagccc gatcacgccc gccccgatca cgccgaggcg
     4201 cttcggcacc ctgtcgatat cgagcgcacc ggcgttgtcg atgatgttct tgccgtcgaa
     4261 cttcgcgaac ggcagctcga tcggcaccga gccggtcgcg atgatcacgt tcacggcctc
     4321 gagcgcctgc ttgttgccgt cctcgccggt gacctcgacc ttgttgcctt tcaggagctt
     4381 gccggtaccg aagaaaggcg tgaccttgtt ggccttgaac agcatcccga cgccgccggt
     4441 gaactgcttg acgatcttgt ccttgcggcc gatgaaggtc gccatgtcga ccttggcgtt
     4501 ctcgacactg atgccgtggg tcggcaggtt gtggctgagg ccccagaatt gcttcgagga
     4561 atcgagcagc gccttggaag gaatgcagcc gacgttgagg cacgttccgc ccagtgcctg
     4621 cttgccgtcc ttgcccttga agttgtcgac gcaggcgact ttcaggccga gctgggccgc
     4681 gcggatcgcg cagatgtagc cggccgggcc agcgccgatc acgatgacgt cgaatttgtt
     4741 gtcagccatt gcagattcct tggaagcggg aacagggaac ggggaacagg gactgagcga
     4801 ggcttttcaa tccccgttcc ctgttccccg ttcccgcatc attacaaacc gagaagcatc
     4861 cggtgcggat tctcgagctg gttcttgatg tcgaccagga acagcaccgc gtccttgccg
     4921 tcgacgatcc ggtggtcgta gctgatcgcg atgtacatca tcggggcggc gacgatctgg
     4981 ccgttctcga ccacggcgcg ttccttgatg gtgtgcatcc cgaggatgcc gctctgcggc
     5041 gggttcacga tcggcgtcga gaacagcgag ccgaaggtgc cgccgttggt gatggtgaag
     5101 gtgccgccct gcaggtcatc gagcgtcagc ttgccggcac gcgcacgttc ggcgaaatcc
     5161 gcgatggcct tttcgatatc ggcgaagccc atgtcctggg cgtcacgcag caccggcgtc
     5221 acgaggccgc gatcggtcgc gaccgcgatc gaaatgtcct ggtagccgtg gtagatgatg
     5281 tcgttgccgt ccaccgacgc gttcaccacc ggatagcgct tcagcgcctc gcacgcggcc
     5341 ttcacgaaaa agctcatgaa accgagcttg atgccgtggg tcttctggaa ctgctcaccc
     5401 aattgcttgc gcatcgcgac cactttcgcg aggttgacct cgttggacga ggtcagcatc
     5461 gcgatcgagt tcttcgattg catcagccgc tcggcgatcc gtgcccgcat gcgggtcatg
     5521 gggacgcgtt cttcggggcg ggagcccggc gtggcgggac cggggaccgg ggaccgggga
     5581 ccggccggcg cgggcattga tgggccgcct ttcgagtagt tgaccacgtc ttccttggtg
     5641 acccgtccac cgcgcccggt tccctcgact ttcgaggtat cgatattctc ttcggtcgcg
     5701 acgcgccgcg cggcaggcga aaggttttcg gagccggctg ttgcttttgc cggtccccgc
     5761 tccccggtcc ccggctccgc cgctttcgcg ggcgcttccg ccgcggtcgg tggctgcggt
     5821 tgtttctctt ccttcttctc cgaagccgcc tgcggcgctt cggccgccgc gccttcctcg
     5881 aggatggcga tcacgtcctg gctgttcacg gtgtcgccgg tgtcgtggcg gatttccttg
     5941 agcacgccgt cggcgggggc cgggacttcc agcaccacct tgtcggtttc gaggtcgacc
     6001 aggttctcgt cgcgcttcac cgcatcgccc gctttcttgt gccacgcggc gatggtggcg
     6061 tcgctgacgg attcggggag taccggtacc ttgacttcgg tcgacatgat gggtccttgg
     6121 tgtagggcgg gcaccgcccg ccgattgtct cgcggcggac ggtgtccgcc ctacggtcgt
     6181 ttattgacgc aggggcctcg cccgccaatg ccgcggcgga catgggtccg cccctgcgat
     6241 ttcgttttcg tgcctcggtt cattccgccg aatggtcgtc acccactggc tcggccagtg
     6301 cctgttgcac cagcgcgtgc tgttgggcga cgtgggtgtc gtggtggccg caggcaggtg
     6361 ccggcgagcg ggcgcggccc gcgtaatgca ggctgcgatt cgcgcccgtg caggcctgca
     6421 ggtggtggcg gatctggaac caggcgccct ggttctgcgg ttcctcctgg caccagatca
     6481 cttccttcgc ggccgggtac ttgtcgagc
//
LOCUS       DASSNH010000206         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1142572, whole genome shotgun sequence.
ACCESSION   DASSNH010000206 DASSNH010000000
VERSION     DASSNH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142572"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFK00_07490"
     CDS             <1..1115
                     /locus_tag="VFK00_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7592829.1"
                     /translation="AQYADAHFQAISAVLSGLADVRKALAEAEGLYAQGRRSVLFVDE
                     VHRFNKAQQDAFLPHIERGVIVFVGATTENPSFELNSALLSRCRVHVMDTVGADDIVV
                     ALKRALADEERGLGALHLQVDDDALMLMAKAADGDVRRGLTFLEIASELAHDGRIEES
                     TLQQVLADRTRRFDKGGEQFYDQISALHKSIRSSDPDAALYWLVRMLDGGCEPLYLAR
                     RMVRMAVEDIGLADPRAWQLALHGWDTYERLGSPEGELALAEVALYLAMAPKSNAAYV
                     AYGAARGTVRETGTLEVPMHLRNAPTKLMKGLGYGKGYQYDPDVKGGVALDQQCLPDA
                     LVGTGFYEPTENGIEAKIRQKLDALREARARARTDG"
     gene            1201..1782
                     /locus_tag="VFK00_07495"
     CDS             1201..1782
                     /locus_tag="VFK00_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592830.1"
                     /translation="MRIAFAALSGAIVIFLWQFVSHMFSPIGEMGFRAPQNENLVLQA
                     AASGLPASGAYLLPYLAPGKMGDPAATQAWTEKAQQNPSLFVVVGPPNTMATNMAPAL
                     IKQFVTNLLGALLVAVVLAATAWSFGMRVLVALAFGIFGWLLDIVPLWTWYQFPPDYI
                     IGNLLDQGIGWLLAGIAMAWLLGRRRAALVPAV"
     gene            1878..3155
                     /gene="serS"
                     /locus_tag="VFK00_07500"
     CDS             1878..3155
                     /gene="serS"
                     /locus_tag="VFK00_07500"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7592831.1"
                     /translation="MLDPVLLRAQLADTAVRLKTRGFALDTTAVESLEAERKRLATET
                     QELQAQRNARSRAIGQAKAKGEDVATIMAEVAGIGDKLKANAAALAEVREKLSAVALG
                     IPNLPDASTPVGKDESENVEVARWGEPRTFGFEVKDHVELGARHGWLDGEAGAKLSGA
                     RFTVLRGQLARLHRALAQFMLDLHTGEHGYTECNVPLLVNPDTMQGTGQLPKFEEDLF
                     STSVGEGKRYLIPTAEVPLTNIVRDSIVEADTLPLRMTAHTPCFRAEAGSYGKDVKGM
                     IRQHQFEKVELVQVVEPAKSMDALEELTGHAERVLQLLELPYRKMALCTGDMGFAAAK
                     TYDLEVWLPSQNTYREISSCSNCTDFQARRMQARWRNPATGKPELVHTLNGSGLAVGR
                     CLIAVMENYQDADGSITVPEALQRYMSDAVRIA"
     gene            3311..3400
                     /locus_tag="VFK00_07505"
     tRNA            3311..3400
                     /locus_tag="VFK00_07505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3345..3347,aa:Ser,seq:tga)
BASE COUNT          649 a         1191 c         1303 g          612 t
ORIGIN      
        1 tcgcgcaata cgccgacgcc cacttccagg cgatctcggc ggtgctgtcg ggtctggccg
       61 acgtccgcaa ggcgctcgcg gaagcggaag gcctgtacgc gcagggcagg cgcagcgtgt
      121 tgttcgtgga cgaggtgcac cgcttcaaca aggcccagca ggatgccttc ctgccgcaca
      181 tcgagcgcgg cgtgatcgtg tttgtgggcg ccaccaccga gaacccctcg ttcgaactga
      241 attcggcgtt gctgtcgcgc tgccgcgtgc acgtgatgga cacggtcggc gccgacgaca
      301 tcgtggtggc gctgaagcgc gcgctcgccg acgaggagcg cggcctcggc gcgctgcacc
      361 tgcaggtcga cgacgacgcg ctgatgctga tggccaaggc cgccgacggc gacgtgcggc
      421 gcggattgac gttcctcgaa atcgcgtcgg aattggcgca tgacgggcgc atcgaggaat
      481 ccacgctgca gcaggtgctg gccgaccgca cccgccgctt cgacaagggc ggcgagcagt
      541 tctacgacca gatctcggcg ctgcacaagt cgatacgctc gtccgatccc gacgccgcgc
      601 tgtactggct tgtgcggatg ctggatgggg gctgcgaacc gctgtacctc gcgcggcgga
      661 tggtgcggat ggccgtcgag gacatcgggc ttgccgatcc gcgcgcctgg cagctcgcgc
      721 tgcatggctg ggatacctac gaacggctcg gttcaccgga aggcgagctc gcgctggccg
      781 aggtcgcgct gtatctcgcg atggcgccca agagcaatgc ggcctacgtc gcctacggcg
      841 ccgcgcgcgg gaccgtgcgc gagaccggca cgctggaggt gccgatgcat ctccgcaacg
      901 cgccgaccaa actgatgaag gggctcggct acggcaaggg ctatcagtat gatccggacg
      961 tcaagggcgg tgtcgcgctg gaccagcaat gcctgcccga tgcgctggtc ggcacggggt
     1021 tctacgagcc caccgaaaac ggcatcgaag cgaagatcag gcagaagctg gacgcattgc
     1081 gcgaggcgcg cgccagggcc cgcacggacg gctgatcgcg ttcgctgcga tgcagcttga
     1141 ccggcgcacc gcgccggcat agcgtgcgct cgccatgccg ggacccttgg gaggtgtgcc
     1201 atgcgtatcg ctttcgcggc gctgtccggc gccatcgtga tcttcctgtg gcagttcgtc
     1261 tcgcacatgt tctcgccgat cggcgagatg ggtttccgcg cgccgcaaaa cgagaacctc
     1321 gtcctgcaag ccgccgccag cggcttgccg gcgtcgggtg cctatctgtt gccctatctc
     1381 gccccgggga aaatgggtga tccggcggcg acgcaggcgt ggaccgagaa ggcgcagcag
     1441 aacccgtcgc tgttcgtcgt ggtcggccca cccaacacga tggccaccaa catggccccg
     1501 gccctgatca agcaattcgt caccaacctg ctgggcgcgt tgctggtcgc ggtcgttctc
     1561 gccgcgaccg catggagctt cggcatgcgc gtgctggtgg cgttggcgtt cgggatattc
     1621 ggatggttgc tggatatcgt gccgctgtgg acctggtacc agtttccgcc cgactacatc
     1681 atcggcaacc tgctggacca ggggatcggc tggctgctgg cgggaatcgc gatggcgtgg
     1741 ctgctggggc ggcgacgcgc ggcgctcgta cccgcggtct gatcggcagc gttcccgggt
     1801 tgccggcgcg ccgcgccggc acggctgcgc gcgttcggcg ataatccgcg ctttgtttcg
     1861 cgacccgagg atcgaacatg ctggatccgg ttctgctgcg cgcgcagctc gccgacaccg
     1921 ccgtgcggct gaaaacgcgc ggcttcgcgc tcgacacgac cgcggtcgag tcgctggagg
     1981 cagagcgcaa gcggctcgcg accgaaaccc aggaactgca ggcgcagcgc aacgcgcgtt
     2041 ccagggcgat cggacaggcc aaggccaagg gcgaggacgt cgcgaccatc atggccgagg
     2101 tcgccgggat cggcgacaag ctgaaggcca acgcggccgc gctggccgag gtgcgggaaa
     2161 agctgtcggc ggttgcgctc ggcatcccca acctgccgga tgcttccacg ccggtcggca
     2221 aggatgaatc ggaaaacgtc gaggtggcgc gctggggcga gccgcgcacg ttcgggttcg
     2281 aggtcaagga tcacgtggaa ctcggtgccc gccacggctg gctggacggc gaggcgggtg
     2341 cgaagttgtc gggtgcgcgc ttcacggtcc tgcgcggcca gctagcgcgg ctgcaccgtg
     2401 cgctcgcgca attcatgctg gacctgcaca ccggcgaaca cggctatacg gaatgcaacg
     2461 ttccgctgct ggtgaatccc gacaccatgc agggcacggg gcaattgccg aagttcgagg
     2521 aagacctgtt ctcgacgagc gtcggcgaag gcaaacgtta tctgatcccc acggcggaag
     2581 tgccgttgac gaacatcgtg cgcgactcca tcgtcgaagc cgacacgctg ccgctacgca
     2641 tgaccgcgca tacgccgtgc ttccgcgcgg aggcaggttc ctacggcaag gacgtcaagg
     2701 gcatgatccg ccagcaccag ttcgagaagg tcgagttggt gcaggtggtc gagccggcga
     2761 agtcgatgga cgcgctggag gaactcaccg gccacgccga gcgcgtgctg caactgctgg
     2821 agttgccgta ccgcaagatg gcgctgtgca ccggcgacat gggcttcgcg gcggccaaga
     2881 cctacgacct ggaagtctgg ttgccgtcgc agaacaccta ccgcgaaatt tcctcgtgct
     2941 cgaactgcac ggatttccag gcccgccgga tgcaggcgcg ctggcgcaat cccgccaccg
     3001 gcaagccgga gctggtacac acgctcaacg gctccggcct cgccgtcggc cgctgcctga
     3061 tcgcggtgat ggagaattac caggacgcgg acggttcgat caccgtgccc gaagcgttgc
     3121 agcgctacat gagcgatgcg gtgcggattg cgtagcgccg ctcggcgcca cgcaagggac
     3181 atcatagaat cgtcattccg caggccggaa tccatttctt gctgcccttg agcttggaac
     3241 aggatcaaaa tggattccgg gttccggccc gatgaagccg ggcctgcccc ggaatgacga
     3301 cgttgaaatc ggagaggtgg ctgagtggtc taaagcaccg gacttgaaat ccggcgtacg
     3361 gcttgccgta ccgtgggttc gaatcccacc ctctccgcca gatacgcgaa cgccccttgc
     3421 gggcgttctg cgtttctgag gaagcgaggt ggtgtggacg aaaccgcttg gggttcgacg
     3481 aatttgtctg gaacaaattt ggacagccga aggcgggccc cgagcgcagc gaggggcgag
     3541 gcgcatggat gcgccgagca atcccacccc ctccgccact caccgctgat cgctttacac
     3601 tgtgggcagg tcagcagcgc gaggtgtgcc atggacgaca agcgcaaacc gaaagtggaa
     3661 ccctacaagg gacctgccgg tggatggggt tccctgaaat ccgtcggcag catcctggcg
     3721 cgggagaagg caccgctcag gaccgcgcgc gagct
//
LOCUS       DASSNH010000207         7393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_232634, whole genome shotgun sequence.
ACCESSION   DASSNH010000207 DASSNH010000000
VERSION     DASSNH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232634"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFK00_07510"
     CDS             <1..338
                     /locus_tag="VFK00_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7592832.1"
                     /translation="LLLNSVLTVRARMAGSHQGKGWEGFTDQVIEKLNRDREGLVFLL
                     WGNYAQAKGRLVDPRRHCILKAPHPSPLSAHRGFLGCRHFSKANQYLEGNGQSPIDWS
                     LPRAARWRA"
     gene            493..1350
                     /gene="rpoH"
                     /locus_tag="VFK00_07515"
     CDS             493..1350
                     /gene="rpoH"
                     /locus_tag="VFK00_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HET7592833.1"
                     /translation="MSEALVANNLPIPSVVGSLDAYIGAVHRIPVLEASEERELARRY
                     HADDDLDAARKLVMSHLRFVVHVARGYNGYGLQLADLIQEGNIGLMKAVKRFDPDHGV
                     RLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKKRLGWMNMEEVRAV
                     AQDLGVPETTVLEMESRLSGRDVGFDPPVEGNDDMPPAPVAYLEDHGADPYLQVADAD
                     QEEHQLDTLQHALAKLDDRTRDIIRRRWLAEDKATLQDLADDYGVSAERIRQLEANAM
                     KKMRGAFEA"
     gene            complement(1625..3826)
                     /locus_tag="VFK00_07520"
     CDS             complement(1625..3826)
                     /locus_tag="VFK00_07520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7592834.1"
                     /translation="MNHLRQSDSPGGIVRVALPVPLPQTFDYLPGAFAAQAGMRVLVP
                     FGRGRRVGIVVECGRGAEVADDRLKPIAAVLDESPLLDPLLLADLRRAADYWCGAIGE
                     VLFGALPLALRRGRPPSDFAEEAWRATAAGTAARDAGTRRGGSAALLATLGDATLAAS
                     ELETLLPGWRAAARRLAAAGLIESTQRTHEPPRAREGAAPILTDDQADALADILSAGP
                     GFHPFLLQGVTGSGKTEVYLRLTERVLATGWQALWLVPEIGLVPQALRRLRARFDAHV
                     AVLHSNLADGDRARAWLDARSGTAAIVLGTRSAVFAPLPRPGLIVVDEEHDTSYKQQE
                     GFRYHARDFALMRAQSLGVPIVLGSATPSLETLANVDAGRYRSVALRHRVRSRPPSPV
                     HVLDLRGLGLAHGLSPALLEALDATVARGEQALVFRNRRGYAPVLLCHACGWHAACPR
                     CEKPLTLYRGRRQLLCHHCGRTEPAPGQCPNCSADALVPQGHGTERLEEALTARYPDV
                     PVLRVDRDSTRRRNAFDKLLDRLAGGAPAILVGTQMLAKGHDLPNLTTVAIVGVDEGL
                     HSADFHAGERLAQLIVQVAGRAGRADKPGTVWLQTHEPSHPLLTTLLKGGYGAAAALQ
                     LGERLASGLPPFGHLALLRAESRRRDVLDGFMAAAREAVVNPGGAIRVNGPVAAPMPL
                     RAGRHRAQLLLESPQRASLRQALTGWLPQLHALPQPRDLRWSIDVDPVELY"
     gene            complement(4129..4434)
                     /locus_tag="VFK00_07525"
     CDS             complement(4129..4434)
                     /locus_tag="VFK00_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HET7592835.1"
                     /translation="MIVYAVELEMDAALREEYLARLQGHVAEMLALPGFLGADVLERV
                     DPPPPAGRCTMSVHYRLRDRAAWEGYLSGHAPRMREAGLRRFGERVRASRQVLRQLA"
     gene            complement(4431..5513)
                     /locus_tag="VFK00_07530"
     CDS             complement(4431..5513)
                     /locus_tag="VFK00_07530"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HET7592836.1"
                     /translation="MQPEISTPPAPPVPATLPPDAPDATPRPVCSNCGAPLYGKYCYA
                     CGQPTVGMVRHFGSILSDVADSLLNIDERLFRTIGPLYLRPGKLTLDYFEGKRARYVT
                     PFRLVFFLAIIAFFAIQLTVRTGYSDLPKKSVVAISTDPGKPNVSVAPMPKPAESEHG
                     MFSNGNLSINGKVIWNRDSKPLQVAWLPDAVVAWINDLVGNAQQQLHAMNEGNYADQQ
                     VARRKFTLGMYSAAPTVLFVLLPLFALLLKIFYIFKRRLYMEHLIVAMHSHAFLMLSM
                     LVLVALGALRHLLVPHAGWLKVPLYLAVAAAWIWIFVYLWLMQKRVYRQGWFMTNLKY
                     WTLGFCYTVLMGFGFVFAVLISLTGA"
     gene            5528..6931
                     /locus_tag="VFK00_07535"
     CDS             5528..6931
                     /locus_tag="VFK00_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7592837.1"
                     /translation="MSSILALDRAHFAREVRATVKLALPLIAGQLSAVGMSAVDVALA
                     GHYNAHTLAAVAVGANVWVLGLVSAVGVMMALSPSVAQLDGANRHREIGPLFRQALWL
                     AAALSVLLWFGARHAGPLLGLIGVDPALTDDVQAFLHALSWGAPGLCGYFALRGLCEG
                     SSWTRPTMLFGLFGLVVLVPLDWIMIYGKFGFPALGARGAGLANALALWLQLLAFAAY
                     LKYGRRFRGLDLFARMERPDWRAIRHLLWIGVPMAVTLLMEAGLFVISALLISRLGTV
                     AIDGHQIAINVSSVTFMVPLGLALAVTVRVGHAAGRGDGRGVRYAGFTGVALTLATQA
                     VSATLMLALPHAIAHIYSDDGRVIALAAQLLILAGIFQFSDGIQVVSNGALRGLKDTR
                     MPMLITIFAYWVVGMPVGAWLAFGAGQGVRGMWFGLIAGLTVAALLLFARWVTQTRGD
                     AWRRFVAASPSPLGTVD"
     gene            7178..>7393
                     /locus_tag="VFK00_07540"
     CDS             7178..>7393
                     /locus_tag="VFK00_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7592838.1"
                     /translation="MTNPPRRGIGSFFRGLGRGINIVRLVIINVVFFGILFLILGLAF
                     RGPPKVGSDTVLLLKPSGMLVEQYSIDP"
BASE COUNT         1168 a         2416 c         2593 g         1216 t
ORIGIN      
        1 tgctgctgct gaactcggtg ctgacggtgc gggcgcgcat ggcggggtcg caccagggga
       61 agggttggga gggtttcacc gaccaggtga tcgagaagct gaaccgcgac cgggagggcc
      121 tcgtcttcct gctgtggggc aactacgccc aggccaaggg ccggctggtc gatccccggc
      181 gccattgcat cctgaaggcg ccgcacccat ctcccctctc ggcccacagg gggttcctgg
      241 ggtgcaggca cttttccaag gccaatcagt atcttgaggg gaatggccag tcgcccatcg
      301 actggtcgct gccgcgcgca gcgcgctgga gggcatgact ttcggcagtt tgtaattcct
      361 gaactggttg gtataattac tcccatgaag cagcggtgac caacgcggtc cttcagggaa
      421 cgtactggga accttgacgg gaatttggcg atcgtattgc tagagtgcaa cacaacgatt
      481 aggggtacaa cgatgagcga agccctggtg gccaacaatc ttccgattcc gagcgtggtg
      541 ggcagcctcg atgcctacat cggcgccgtg caccggatcc cggttctgga agcgagcgaa
      601 gaacgcgaac tcgcgcgccg ctaccacgcc gacgacgatc tcgatgccgc gcgcaagctg
      661 gtgatgtcgc acctgcgttt cgtggtgcac gtcgcccgcg gctacaacgg ttacggcctg
      721 caactcgccg acctgatcca ggaaggcaat atcggcctga tgaaggccgt caagcgtttc
      781 gatccggacc acggcgtgcg tctggtcagt ttcgcggtgc actggatccg cgccgagatg
      841 cacgaattca tcctgaagaa ctggcggatc gtcaaggtcg ccaccaccaa ggcccagcgc
      901 aagctgttct tcaacctgcg caagtccaag aagcgcctcg gttggatgaa catggaggag
      961 gtgcgcgcgg tggcccagga tctcggggtg cccgagacga ccgtgctgga aatggaatcg
     1021 cgcctgtccg gccgcgacgt cgggttcgac ccgccggtcg agggcaacga cgacatgccg
     1081 cccgcgccgg tcgcgtacct cgaggaccac ggggccgatc cgtacctgca ggtcgcagac
     1141 gccgaccagg aagagcacca gctcgatacc ttgcagcacg cgctggcgaa gctggacgac
     1201 cgcacccgtg acatcatccg ccgccgctgg ctggccgagg acaaggccac gctgcaggat
     1261 ctcgccgacg attacggcgt gtcggccgag cgcatccggc agctcgaagc caatgcgatg
     1321 aaaaagatgc gcggtgcgtt cgaggcttga gggcagggag cagggaagag ggagcacggc
     1381 cgaagcggtc cacttcctca tctgaaaaaa gcgcggcgcc tgccgcgctt tttttgtgct
     1441 ggtcttcccc cttccgaagg taggcggagc gaagcgaacg cccgcagcgc gacgagtggc
     1501 atggcttccg gagcggtgac gccgccgggc atccccccga agccgccggc ggcggcttcg
     1561 acccccttcc ttcggaaggg ggtgttcggc gcgtttccct gctcccttcg ccctgctccc
     1621 tgcttcaata caattccacc ggatcgacgt cgatcgacca gcgcaggtcg cgcggttgcg
     1681 gcagcgcatg caactgcggg agccatccgg tgagcgcctg ccgcagcgac gcgcgttgcg
     1741 gcgattcgag cagcagttgc gcgcgatggc gccccgcgcg tagcggcatc ggcgccgcca
     1801 cgggtccgtt gacgcgaatg gcgccgccag ggttaaccac ggcctcgcgc gcggcggcca
     1861 tgaaaccgtc cagcacgtcg cgccggcggc tttccgcgcg cagcaaggca aggtgaccga
     1921 acggcggcaa ccccgaagcc aggcgttcgc cgagctgcag cgccgccgcg gcgccgtagc
     1981 cgcccttgag cagggtggtg agcaacggat gcgaaggctc gtgggtctgc agccaaaccg
     2041 tccccggttt gtcggcgcgc ccggcgcgcc ccgcgacttg cacgatcagc tgcgcgagcc
     2101 gttcgccggc gtggaagtcg gcgctgtgca ggccctcgtc cacgccaacg atcgccacgg
     2161 tggtgaggtt cggcagatcg tggcccttgg ccagcatctg ggtgccgacc aggatcgcgg
     2221 gcgcgccgcc ggcgagccgg tcgagcagct tgtcgaacgc attgcggcgg cgggtgctgt
     2281 cgcggtccac gcgcagcacc ggcacgtccg ggtagcgcgc ggtcagcgct tcctcgagcc
     2341 gctcggtgcc gtgcccctgc gggaccagcg cgtcggcact gcaattcggg cactgccccg
     2401 gcgcgggttc cgtgcgcccg cagtggtggc agagcagttg ccggcggccg cgatagagcg
     2461 tcaacggctt ctcgcagcgc gggcaggccg cgtgccagcc gcaggcgtgg cacagcagca
     2521 ccggtgcgta gccgcgtcgg ttcctgaaga ccagcgcctg ctcaccgcgc gcgacggtgg
     2581 cgtccagcgc ttccagcagg gccggtgaca agccgtgcgc gagcccgagg ccgcgaaggt
     2641 ccagcacgtg caccggcgac ggcggccgcg accgcacgcg atggcgcagg gcgacgctcc
     2701 ggtagcgccc ggcgtcgaca tttgcgaggg tctcgagcga cggcgtcgcg ctgcccagca
     2761 cgatcggtac gcccagcgac tgggcgcgca tcagcgcgaa gtcgcgcgcg tggtagcgga
     2821 acccttcctg ctgcttgtag gaagtgtcgt gttcctcgtc caccacgatc aggcctgggc
     2881 ggggcagggg cgcgaacacg gccgagcgcg tgccgaggac gatggcggcg gtgccggaac
     2941 gtgcatccag ccaggcgcgg gctcggtcgc cgtcggcgag gttcgagtgc agcaccgcga
     3001 cgtgtgcatc gaagcgtgcg cgcaaccgcc ggagcgcctg cggcaccagg ccgatctcgg
     3061 gcaccagcca caaggcttgc cacccggtgg cgagcacgcg ctcggtcagt cgaaggtaca
     3121 cctcggtctt gccgctgccg gtgacgccct gcaacaggaa cggatggaag cccgggccgg
     3181 cggacaggat gtcggcgagc gcgtcggcct ggtcgtcggt caggatcggc gcggcgcctt
     3241 cacgggcccg cggcggctcg tgggttcgtt gcgtgctctc gatcaggccc gcggccgcca
     3301 gccgccgcgc cgccgcgcgc cagccgggca gcagggtttc gagttcgctc gcggcgagcg
     3361 ttgcatcgcc cagggttgcg aggagcgccg cggaaccgcc gcgccgggtg ccggcatccc
     3421 gcgccgcggt tccggcagcc gtggcacgcc aggcttcctc ggcgaagtcc gagggcgggc
     3481 gcccgcgacg cagcgcgagt ggcagcgcac cgaacagcac ctccccgatc gcgccgcacc
     3541 agtaatcggc cgcacggcgc aggtcggcga gcagcaacgg gtcgagcaat ggcgattcgt
     3601 ccagcaccgc ggcaatcggc ttcaggcgat cgtcggcgac ttcggcgccg cgtccgcatt
     3661 ccacgacgat gccgacccgc cggccgcgcc cgaacggcac gagcacgcgc atgccagctt
     3721 gcgccgcgaa cgcgccgggc aggtaatcga aggtttgcgg cagcggcacc ggcagcgcca
     3781 cccgcacgat tccgcccggt gaatcggatt gccgcaggtg gttcatgact tacgggaggt
     3841 tgttgtcatg cgaggcagga ttgtccgata agtgagtgag gcgttggcgg ttttttgctt
     3901 atccacaatc attgtggata acgttgtgga ggaacggcgg gcaagcctcg cgcgggctca
     3961 tggaatcgga ccgcggcacg acttggcgaa attttcagca accgtggttt cgctagcaaa
     4021 ttcagtgaca tgcgatcagc aacgccgcgc aaaggacggc caagcgtgtt ccaggcgtcg
     4081 ttccggcgcg cgtttttgcg gctgtgcaca acggccggcg atgccgggtc acgcgagttg
     4141 ccggaggacc tgccgcgagg cccgcacccg ctcgccgaaa cgcctcaacc cggcttcgcg
     4201 catgcgtggc gcgtggccgg acagatagcc ctcccatgcc gcccggtcgc gcagccgata
     4261 gtgcaccgac atcgtgcagc gtccggccgg cggtggcgga tccacgcgct ccagcacgtc
     4321 ggccccgagg aagcccggca aggccagcat ttccgcgaca tggccctgca gcctcgcgag
     4381 gtattcctcg cggagggccg cgtccatttc gagctcgacg gcataaacga tcatgcaccg
     4441 gtcaggctga tcaataccgc gaatacgaat ccgaacccca tcaggaccgt gtagcagaac
     4501 cccagggtcc agtacttcag gttggtcatg aaccagccct ggcggtagac gcgcttctgc
     4561 atcagccaca agtacacgaa gatccagatc cacgcggccg cgacggccag gtacaaggga
     4621 accttgagcc agccggcatg cggcaccagc aggtggcgca atgccccgag cgccaccagc
     4681 accagcatcg acagcatcag gaaggcgtgg ctgtgcatgg cgacgatcag gtgctccatg
     4741 tacagcctgc gcttgaagat gtagaagatc ttcaacagca gcgcaaacag cggcagcagc
     4801 acgaacaaca ccgtgggcgc ggccgagtac atgccgagcg tgaatttgcg ccgcgcgacc
     4861 tgctggtccg cgtagttgcc ttcgttcatc gcgtgcaact gctgctgcgc attgccgacc
     4921 aggtcgttga tccaggcgac gacggcgtcc ggaagccagg cgacctgcag cggcttgctg
     4981 tcgcggttcc agatcacctt gccgttgatc gacagattgc cgttggagaa catgccatgc
     5041 tccgactccg ccggcttcgg catcggcgcc accgaaacgt tcggcttgcc gggatccgtc
     5101 gagatggcaa caaccgactt tttcggcagg tcgctgtagc cggtccggac ggtcaactgg
     5161 atcgcgaaga acgcgatgat cgcgaggaag aacaccagcc ggaagggcgt gacgtagcgg
     5221 gcgcgcttgc cctcgaaata atcaagcgtc agcttgcccg gccgcaggta caatggaccg
     5281 atggtgcgga acagccgctc gtcgatgttg agcaggctgt cggcgacgtc gctcaggatg
     5341 ctgccgaagt ggcgcaccat gccgacggtc ggctgcccac aggcgtagca gtacttgccg
     5401 tagagcggcg cgccgcagtt gctgcaaacc ggccgcggcg tggcgtccgg cgcgtcgggc
     5461 ggcagcgtgg ccggtacggg cggcgctggc ggtgtggaaa tttccggttg catcgggtcg
     5521 tcccgcaatg tcttcgattt tagccctgga tcgcgcgcat ttcgcgcgcg aggtccgcgc
     5581 gacggtgaag ctcgcgctgc cgctgatcgc cggccaattg tcggcggtcg ggatgagtgc
     5641 ggtcgacgtt gcgctggccg gacactacaa cgcccacacc ttggccgcgg tggcggtcgg
     5701 cgcgaacgtg tgggtgctgg gactggtgag cgcggtcggg gtgatgatgg cgctgtcccc
     5761 ttcggtcgcg cagctcgacg gtgcgaaccg ccatcgcgaa atcgggccgt tgttccggca
     5821 ggcgctgtgg ctggccgcgg cgctgtcggt gctgctctgg ttcggggcgc gccacgcggg
     5881 cccgctgctg ggcttgatcg gcgtcgatcc ggccttgacc gatgacgtgc aggcgttcct
     5941 gcacgcgctc agttggggcg cgcccggcct gtgcggctat ttcgctctgc gtggcctgtg
     6001 cgaaggctcg agctggacgc gccccacgat gctgttcggc ctgttcggcc tggtggtgct
     6061 ggtgccgctg gactggatca tgatctatgg caagttcggc tttccggcgc tgggtgcgcg
     6121 cggcgcgggc ctcgccaacg cgctcgcgtt gtggttgcag ctcctcgcct tcgccgcata
     6181 cctcaaatac ggcaggcgat tccgtggcct cgacctgttc gcgcggatgg agcggcccga
     6241 ctggcgggcg atccggcatc tcctgtggat cggtgtcccg atggcggtga cgctgctgat
     6301 ggaagccggc ttgttcgtga tttcggcgct cctgatcagc cggctcggca ccgtcgcgat
     6361 cgacggccac cagatcgcga tcaacgtttc ttcggtgaca ttcatggtgc ccttgggcct
     6421 cgcgctggcc gtgacggttc gggtcgggca tgccgccggg cgcggcgacg ggcgcggggt
     6481 gcgctacgcg ggcttcaccg gcgtggcgct gacgctggcc acccaggccg tctcggcgac
     6541 gctgatgctg gcgctcccgc acgcgatcgc gcacatctat tcggacgatg gacgcgtcat
     6601 cgcgctggct gcgcaattgc tgatcctggc ggggatcttc cagttctccg atgggatcca
     6661 ggtggtgtcc aacggtgcgc tgcggggctt gaaggacacc aggatgccga tgctgatcac
     6721 gatcttcgct tactgggtcg tgggcatgcc ggtgggcgcg tggctggcgt tcggcgccgg
     6781 ccagggcgtg cgcggcatgt ggttcgggct gatcgcgggc ctgaccgtgg cggcgctgtt
     6841 gctgttcgcg cgctgggtaa cgcagacgcg cggcgatgcc tggcggcggt tcgtggccgc
     6901 gtcgccttcg ccgcttggca ccgtcgactg atccgcccgc cggcgcctgc ggtgtttact
     6961 tctcctctcg ggagcaggtg gcgcgcagcg gcggatcagg gtccgagcgc aggagcgaca
     7021 tcggcagtcc tgttgcacag cacggacgcg cacccccacc cacccctgcg ggaaagcgtt
     7081 ctcagcgacc tggattcaat gcaccggcca attgcgtgac gcgcaccccg cggtgggata
     7141 gtctggaaac atcggacttc tgttgcagga tcggcgcatg accaacccac cacggcgcgg
     7201 aataggaagc ttcttccgcg ggctcgggcg cggcatcaac atcgtgcgcc tcgtgatcat
     7261 caacgtggtg ttcttcggga tcctgttcct gatcctgggg ctggcgttcc gcggcccgcc
     7321 caaggtcggg tccgacacgg tgctgctgct gaagccgagc gggatgctgg tcgagcagta
     7381 cagcatcgat ccg
//
LOCUS       DASSNH010000208        14039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1048716, whole genome shotgun sequence.
ACCESSION   DASSNH010000208 DASSNH010000000
VERSION     DASSNH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048716"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VFK00_07545"
     CDS             <1..1718
                     /locus_tag="VFK00_07545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET7592839.1"
                     /translation="LLLESEHIDCLLQASDTLVAMIQALRDGENVPSPPAELMERLQP
                     AAGEASPAAAAAPESAATAEGAAGQGMGDDEFERLLDDLHGQGNAPVAQGVAPSGSID
                     DAEFEALLDSIYGKGGAPGVAEGGPEGSESSTDNVIPAKAVIHPDSEVDSKMDSGLRR
                     NDEQKKPSGDATAAESQKHRADTTIRVDTHRIDALINAAGELVLVRNRLANLAARGAN
                     SEMERAVGNLDRVAGDLQAAALRMRMQPIGRLFQRFPRIVRDLARQLGKQVTLVQDGE
                     DTNLDRTLVESLADPLVHLLRNAIDHGLEDPDTRAEAGKPRQGTVTLAAGQEGERIVI
                     RISDDGRGMDPEVLRRKAVEKGLISAAQADQLEPAECHALIFHPGFSTRSEISDISGR
                     GVGMDVVKTGIANLGGTIAIESTPGLGTTIHIAIPLTLAIVRVLMVRVGERLLALPLT
                     NVLEVFELHKTQMRMLDGRVVAAHRGRPLPLSDLAAWAGVPAGPETRAQVVVVHVGHQ
                     QLGLLATEVHGREEVMIKPLGPRLRHLPGIAGATITGNGRVALVLDLVSLAESSLPQP
                     LRMTA"
     gene            1723..2463
                     /locus_tag="VFK00_07550"
     CDS             1723..2463
                     /locus_tag="VFK00_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HET7592840.1"
                     /translation="MDPLSLIGAILAFMVLVFGIILKGGTVAELWNLAALIIVFGGTL
                     SATILHTPLPVVRRALSIVRWVFRPPHLHAPDLVKRIVGWSELSRRQGLLGLEPNIES
                     ESDPLTKKGLQLLVDGSEPETIRSVLEVEISTREHVDMDAAKVFEQAGVYSPTMGIIG
                     AVMGLMAVMQNLADPSKLGSGIAAAFVSTIYGISLAYLFMLPMSAKLKSITRNRARLD
                     ELLVEGLISIAQGDNPRQIESKLKGFLP"
     gene            2460..3401
                     /gene="motD"
                     /locus_tag="VFK00_07555"
     CDS             2460..3401
                     /gene="motD"
                     /locus_tag="VFK00_07555"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HET7592841.1"
                     /translation="MRKPHQEEHVDAEKWAIPYADLLTLLLAFFVVMYSLSSVNEGKF
                     RILAESMRAAFHGTGQVIGDVKVERAHQAKLPNEKHATGALPIARITVPVPPRQPPPP
                     GHEGAQTRPGGAGEDQTATLKAIANNVAKALQPWVASKQVVIRKTEQWLEIEIRTDVL
                     FPSGVAQLAEPARKVLDGVAGALAPFPNPIRIEGYTDNRPIDTWKFPSNWELSAARAG
                     SVARLFSDHGVDSGRIAIFGWGDTHPIASNATPEGRNRNRRIAIVVLGDQGVPKRYYS
                     SDPHSLAANSDQPSTATQGAAPDMASSIQAASMHGVQ"
     gene            3427..3894
                     /locus_tag="VFK00_07560"
     CDS             3427..3894
                     /locus_tag="VFK00_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7592842.1"
                     /translation="MNADSPTGSGSWLAFELAGQAYAVPLADVHEIIRPEPPAPVPGA
                     PAEVLGIVSLRGSIVTVLDGCRRLGLEGGKHDPGERLVVFRGEDENVGLRIDALRDVM
                     DLDLAGLMPPPPGTASRDNDPVLGVLDCGDGFVTLLDAGKLCRPHQPAMEAST"
     gene            3891..4274
                     /locus_tag="VFK00_07565"
     CDS             3891..4274
                     /locus_tag="VFK00_07565"
                     /inference="COORDINATES: protein motif:HMM:NF022428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HET7592843.1"
                     /translation="MNIVDTNIVLWGILLGVLVLLLVVCVVAWWQLARRLHAIAEAQQ
                     QFASTDVPTSMLVTAMARVEARLQQIEHERQIAPAPQAQPDDRAYDLAQRMARNGADA
                     AQIAEACGINLHEAELLRRLHAPAG"
     gene            4506..6149
                     /locus_tag="VFK00_07570"
     CDS             4506..6149
                     /locus_tag="VFK00_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET7592844.1"
                     /translation="MFVGIFALTACTAAAAATPPSFSTQRLSREVKTLSSDAFEGRGP
                     ATPAEDKTIDYVVARFKAAGVQPGGDLKDGKRTWTQAVPLLRSDIVGTPSLTLTVDGK
                     ARKLTQGEEIAIRSPLDGSKSVDVANLPLVFVGYGVAAPERNWDDYKGVDLHGKIAVV
                     LVNDPDFETGHGEFGGKAMTYYGRWTYKYEEGARRGAAGVLVVHETAPASYGWATVKN
                     SNTNSMFDIVRKQPAKAHTQLEGWIQHDLAVSMFKQAGLDFDALKKQAQTRDFKPVAL
                     KGETLGARFKVDSRVITSHNIVARIEGSKYPDQTVIYSAHWDHLGVGKPDASGDRVYN
                     GAVDNATGTAALIEMARAFAHGPRPERSVVFLNVTAEEKGLLGSEYYAANPLYPLATT
                     AGVLNMDALSPMGPTRDFTISGTAKLGLLDDLIAAGKAFGMTYTPDSHPEAGYFFRSD
                     HFSFAKRGVPAVSFGGGEDLVDGGVKAGRAAAADYTAHRYHQPGDEWQANWPFTGMAR
                     DLQLLYTVGSRLAHSQEWPNWGKDSEFRAMRDASEAERS"
     gene            complement(6300..6677)
                     /locus_tag="VFK00_07575"
     CDS             complement(6300..6677)
                     /locus_tag="VFK00_07575"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET7592845.1"
                     /translation="MSDKIFTVWKEFRFDAAHQLDAGPAGDPRYRRMHGHSYQVEVWL
                     RGPLTERGWVIDMGDLERRIEVARDALDHRLLNDVEGLGIPTMENIAAFVWDKLGELP
                     QLQRVVVKRTQNGEGCEYRGPGK"
     gene            complement(6674..7306)
                     /gene="queE"
                     /locus_tag="VFK00_07580"
     CDS             complement(6674..7306)
                     /gene="queE"
                     /locus_tag="VFK00_07580"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HET7592846.1"
                     /translation="MYAIKEIFHTLQGEGFHAGRPAVFCRFAGCNLWSGREADRAAAA
                     CNFCDTDFVGTDGDNGGKYPDAAAVAEKIASLWPADAPQNRFVVFTGGEPLLQLDTTL
                     LEAMHVRGFECAVETNGTLEAPEGLDWITVSPKGTQPVKLRRADELKLVYPQSNAPPE
                     RFTGFDAGHFFLQPMDGPRARENTRATVDYCKAHPRWRLSLQTHKYLGIP"
     gene            7408..8214
                     /locus_tag="VFK00_07585"
     CDS             7408..8214
                     /locus_tag="VFK00_07585"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7592847.1"
                     /translation="MLQREPPSALRSFVGMLWAGEPAAACRPVRERVLPTGRMHLVFR
                     ASTQPLRILGEPQASSGGTFCHAIVGGARACAYLREVAPGGASVGAELCAGASMALFG
                     VPADALAARHTPLEDLWGAAAMRACDRIFSAPDAARQLDVFAGILAARLPRVSGLHPA
                     VASALAASRECGEVHAMVAASGYSHRHFTAVFERETGLTPKRFCRVQRFGRLLAHAAA
                     RPALSWTELAMAGGYSDQPHFNREFHAFAGITPGEYRRAAPRYPHHVPID"
     gene            8322..8765
                     /locus_tag="VFK00_07590"
     CDS             8322..8765
                     /locus_tag="VFK00_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7592848.1"
                     /translation="MAIHEVFAYLTVDGAAEAIDFYVRAFGAREKFRLAEPGGRIGHA
                     ELDFGGTVVMLADTFPEFGLRDPKALGGTPASIHLHVDDADAMIRRAVDAGATLEREP
                     TDHFYGERAGSVFDPFGHRWLVGHSIEDVPPEEMQRRYTAMVQGQ"
     gene            8792..9289
                     /locus_tag="VFK00_07595"
     CDS             8792..9289
                     /locus_tag="VFK00_07595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592849.1"
                     /translation="MQIRTARVADAEAIARGEAETARMPGLLVGRPGEIPLDAYRAKI
                     AKLESQGRYIVAEHDGAVIGHAFLDPMPMRANAHVHLLTIVVHPAHIGRGIGRAMLRD
                     LLEWAQGDRRVGKVELNVRAGNLRAQNLYRSMGFVEEARFRGRVQRADGQLEDDVGMA
                     WFPER"
     gene            9344..9772
                     /locus_tag="VFK00_07600"
     CDS             9344..9772
                     /locus_tag="VFK00_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET7592850.1"
                     /translation="MADRVEDARALDTLRQMAALQARVCYMNVDRTLATWTRSALTLI
                     VFGTVVDRYGLLLLRDHFPHVGTRLAPNPVSSAGGLLLVAIGVFIAIAAAIRHQRYKA
                     TWHRAFGRDETFGPWLAFPFAVGVAMLGVTILALLLVLVQ"
     gene            9812..10243
                     /locus_tag="VFK00_07605"
     CDS             9812..10243
                     /locus_tag="VFK00_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET7592851.1"
                     /translation="MADDAHNLQVEAARELVELSLQRSYMNTERTLAVWTRTALSLMI
                     FGIAVDRFGLLMRHPPWHVLDTRQLLPDPLSTLGGVVLVAMGVAILLAAGFRFLAYGL
                     KWYRRNPRKVWFHTPWLAFSFAMLMAAFGIALLAVLLIFQQ"
     gene            complement(10297..10824)
                     /locus_tag="VFK00_07610"
     CDS             complement(10297..10824)
                     /locus_tag="VFK00_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592852.1"
                     /translation="MMSKSGRVAATAVAAGALLLAACSNPPLPPPASERNSPTAMVAA
                     IRAAGAADKSVVQVAPLRDPAVDGFLGQAHAAENAGNYQDALDRIDAALKLSPDAPDI
                     LQFRAEVEILLRNYPAAEADARHSFMLGPKVGSLCASNWQTILEVAEAQDRQADVTTA
                     RAARDKCHKAGPVRM"
     gene            complement(10842..13154)
                     /gene="mrcB"
                     /locus_tag="VFK00_07615"
     CDS             complement(10842..13154)
                     /gene="mrcB"
                     /locus_tag="VFK00_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HET7592853.1"
                     /translation="MRRVWPFARWPILIGIGLAVGFVVPYTLVLDHRVKTRVSEIDFT
                     QPTRVYARPQLLAAGVPMDKATLKLELGMAGYTEAPSSARVPGTWSADGGTFVIASRG
                     YMDPVGGELPRRIRVRLGSGRIQSVFDITANKPLHKTHLDPARIATMYGARQEERIIL
                     PLSEMPPLLVQGVQAVEDRGFKNNHGIDLSSIVRAALADLRAGRVVQGGSTITQQLVR
                     NLFLDRSQTLVRKFNEALLSILLDAHFSKGEILNAYCNEVFLGQQGNQVVRGFGAASW
                     YYFGRPLQTLRPQEIALLVGMVQGPSYYEPRRNPQRALARRNVALAAFHATGLLNDAQ
                     WQAAKSAPLDVTPKPQLVVDRFPAFMQVVREQIARDFGEQQLRNGGLSVYTTLDPAAQ
                     VYAEQSLDEAIKGLGKRGNQLEGAVVVVEPSTGSVLAMVGGRESDLHGFNRAYDARRP
                     VGSSLKPFYYLMALTDPSRWNVASLLDDSPISLKLRNGKSWTPHNDDNRSHGEVPMVE
                     ALAKSYNLASVHLGLQLGVDRVAAFLRSFGLEGVQPNPSLLLGAIDMSPFQLAQLYEF
                     FAGDGHALPLLTLRGVLDRSGHVLKQYEVKPGKGQYRQAERLVRWMMQQVTQYGTAAA
                     IGDHPELADLHAAGKTGTSDHQRDSWYSGFTGDRLAVAWMGRDDNKPTHLWGATGALK
                     VWMKLFEKMPSTPLDTSFGDGIQYAWIDPSTGQGTLPQCDGAREYPFIDGYLPQVQNH
                     CYLQRLEHIFSSGDSGDGSSQPSAGARAWR"
     gene            complement(13293..13826)
                     /locus_tag="VFK00_07620"
     CDS             complement(13293..13826)
                     /locus_tag="VFK00_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HET7592854.1"
                     /translation="MRSVPVVLAGFAWMFAAAATAQVTYSNRPPPPGYTGNPGQVVAC
                     GSPQYHLARCPVPPGWRGARLVRQTSDSACVEGRTWGFDRGSIWVNEGCGGEFAAVGG
                     WRPGPGWDRDFVVSCGSPQYRRNFCQVDVGARGHVRLQRQLSDSACIEGRSWGWNRAG
                     IWVDQGCGASFLVTRRW"
BASE COUNT         2283 a         4868 c         4702 g         2186 t
ORIGIN      
        1 gcctgctgct ggaatccgag cacatcgact gcctgctcca ggcatcggac accttggtgg
       61 cgatgatcca ggcgttgcgc gacggcgaaa acgtgccctc gcctcccgcg gagttgatgg
      121 aaaggctgca acccgcggcg ggcgaagcgt ccccggcagc agccgccgcg ccggaatcgg
      181 ctgcgacggc tgagggtgcg gccggccagg gcatgggcga tgacgaattc gaacggctgc
      241 tggacgacct gcacggccag ggcaacgcgc ccgtggcaca gggcgtcgcg ccctccggaa
      301 gcatcgacga tgccgagttc gaggcgctcc tcgacagcat ctacggcaag ggtggcgcgc
      361 ccggcgtggc cgaaggcggt cctgagggca gcgaaagctc caccgataac gtcatcccgg
      421 cgaaggccgt gattcatcct gattctgaag tcgacagcaa aatggattcc ggcctgcgcc
      481 ggaacgacga gcaaaagaaa ccgtccggcg acgcaacggc tgcggaatcc caaaaacacc
      541 gcgccgacac caccatccgc gtcgacaccc accgtatcga cgccctgatc aatgccgccg
      601 gcgaactggt gctggtgcgc aaccggctcg cgaacctcgc cgcgcgcggt gccaacagcg
      661 aaatggaacg cgccgtgggc aacctcgacc gtgtcgcggg cgacctccag gcggcggcac
      721 tgcggatgcg gatgcagccg atcggccggc tgttccagcg tttcccgagg atagtccgcg
      781 acctcgcgcg ccagctcggc aagcaggtga cgctcgtcca ggacggcgag gacaccaacc
      841 tcgaccgcac cctggtcgag tcgctggccg acccgctggt ccaccttttg cgcaacgcca
      901 tcgaccacgg gctcgaggat cccgacaccc gtgccgaggc cggcaagccg cgccagggca
      961 cggtgacact cgcagccggg caggaaggcg agcgcatcgt gatccggatc agcgacgacg
     1021 gccgcggcat ggacccggag gtgttgcgcc gcaaggcggt cgagaaagga ttgatcagcg
     1081 ccgcgcaggc ggaccagttg gaacctgccg aatgccacgc cctgatcttc caccccggtt
     1141 tcagcacgcg cagcgagatc tccgacattt ccggccgcgg cgtcggcatg gacgtggtca
     1201 agaccggcat cgccaacctc ggcggcacca tcgcgatcga atcgacgccc ggcctcggca
     1261 cgacgatcca catcgcgatc ccgctgacgc tggcgatcgt ccgcgtgttg atggtgcggg
     1321 tgggcgagcg cctgcttgcg ctgccgctca ccaacgtgct cgaagtcttc gaattgcaca
     1381 agacccagat gcggatgctc gatggccggg tcgtcgccgc acaccgcggg cgcccgctgc
     1441 cgctcagcga cctcgccgcg tgggcaggcg tcccggcggg cccggaaacc cgcgcccagg
     1501 tcgtggtggt gcacgtcggc caccagcagc tcgggctgct cgcaaccgaa gtgcacggcc
     1561 gtgaagaagt catgatcaag ccgctggggc cgcgtcttcg gcacctccca ggcatcgccg
     1621 gcgccacgat tacgggcaac ggccgcgtcg cgctggtgct cgacctggtg tcgctggccg
     1681 aatcctccct cccccaaccc ctgcggatga cggcctgacg ccatggaccc cctgagcctc
     1741 atcggcgcca tcctcgcctt catggtcctg gtcttcggca tcatcctgaa gggcgggacg
     1801 gtcgcggagc tgtggaacct ggccgcgctg atcatcgtgt tcggcggcac cctctcggcc
     1861 acgatcctgc atacgccgct gccggtggtg cggcgcgccc tgtcgatcgt ccgctgggtg
     1921 ttccggccgc cgcacctgca tgcgcccgac ctggtgaaac gcatcgtcgg ctggagcgaa
     1981 ctgtcgcgcc gccagggcct gctgggcctc gagccgaaca tcgaatccga atccgaccct
     2041 ctcacgaaaa aaggcctgca gctgctcgtc gatggcagcg agccggaaac catccgcagc
     2101 gtgctggaag tggaaatcag cacgcgcgaa cacgtcgaca tggacgcggc gaaggtgttc
     2161 gagcaggccg gggtgtactc gcccaccatg ggcatcatcg gcgcggtgat gggcctgatg
     2221 gccgtgatgc agaacctggc ggaccccagc aagctcggca gcggcatcgc ggccgcattc
     2281 gtgtccacga tctacggcat cagcctcgcg tatctcttca tgctgccgat gtcggccaag
     2341 ctgaagagca tcacccgcaa ccgtgcgcgg ctcgacgagc tgctggtcga gggcctgatc
     2401 tcgatcgcgc agggcgacaa tccgcgccag atcgaaagca agctcaaggg cttcctgcca
     2461 tgaggaagcc gcaccaggaa gagcacgtcg acgccgagaa gtgggcgatc ccctacgccg
     2521 acctcttgac cctgctgctc gcgttcttcg tggtgatgta ttcgctgtca tcggtcaacg
     2581 aggggaagtt ccggatcctc gccgaatcga tgcgcgcggc gttccacggc accggccagg
     2641 tgatcggcga cgtcaaggtg gaacgtgcgc accaggccaa gctgccgaac gagaaacacg
     2701 cgaccggtgc cctccccatc gcgcgcatta ccgtgccggt gccgccgcgc cagccgccgc
     2761 cgcccggcca tgagggcgcg caaaccaggc ccggcggggc gggtgaggac cagacggcca
     2821 cgctcaaggc catcgccaac aacgtcgcca aggcgttgca gccatgggtc gccagcaagc
     2881 aggtcgtgat ccgcaagacc gagcagtggc tcgaaatcga gatccgcacc gacgtgttgt
     2941 ttccgagcgg cgtcgcgcaa ctcgccgaac cggcgcgcaa ggtcctcgac ggcgtggcgg
     3001 gcgcgctcgc gcccttcccc aacccgatcc gcatcgaggg ctataccgac aaccgcccca
     3061 tcgatacctg gaagtttccg tcgaactggg agctctcggc cgcgcgcgcc ggcagcgtcg
     3121 cgcgcctgtt cagcgaccac ggcgtcgatt ccggccgcat cgcgatcttc ggctggggcg
     3181 acacccaccc aatcgccagc aacgcgaccc ccgaaggccg caaccgcaac cgtcgcatcg
     3241 cgatcgtggt gctgggcgac cagggcgtgc cgaagcgcta ttacagcagc gatccgcatt
     3301 ccctcgccgc gaattccgac caaccatcca cggcgacgca gggcgccgca ccggacatgg
     3361 catcctcgat ccaggctgcc agcatgcacg gcgtgcaatg atcgaggtcc ggggagatcc
     3421 atcggcatga acgcggacag cccgactggc agcggttcct ggctcgcctt cgaactggca
     3481 ggccaggcct acgccgtgcc gctcgccgac gtgcacgaga tcatccgccc cgagccgccg
     3541 gcgccggtac cgggcgcacc cgccgaggta ctcggcattg tcagcctgcg cggcagtatc
     3601 gtcaccgtac tcgatggctg tcggcgcctg ggcctcgagg gcggcaagca cgatcccggc
     3661 gagcggctgg tggtctttcg cggcgaggac gaaaacgtcg gcttgcgcat cgatgcgctg
     3721 cgcgacgtga tggatctcga cctcgccggg ctgatgccgc cgccccccgg aacggccagc
     3781 cgcgacaacg acccggtgct cggggtgctg gactgcggcg acggattcgt tacgctgctg
     3841 gacgcgggca agctgtgccg tccgcaccaa cccgcgatgg aggcatcgac gtgaacatcg
     3901 tcgacacaaa cattgtctta tggggcatcc tgctgggcgt cctggtgctg ctgttggtgg
     3961 tgtgcgtcgt ggcgtggtgg caactggcgc gccggctgca cgccatcgcc gaagcccagc
     4021 agcaattcgc cagcaccgac gtgcccacct cgatgcttgt gaccgcgatg gcacgggtcg
     4081 aggcgcgcct gcagcaaatc gaacacgaac gccagatcgc gcccgcgccg caggcgcagc
     4141 ccgacgaccg cgcctacgac ctcgcgcaac gcatggcacg aaacggcgcc gacgccgccc
     4201 agatcgccga agcctgcggc atcaacctgc acgaagccga actgctgcga cggttgcacg
     4261 cgccggcggg ctgagccgca ccgccgcagc ggccagtcgc atgtgattcc gcggcgacgc
     4321 atgctggctc gcatcgccac cgcggcgtaa ccacactgga tcccggatat cgcttcgcga
     4381 ttccgggatg gccagcaaaa accggggttc gctggagatt gtcgaacggt agtgcggccg
     4441 cgctgcctgc gggtttgcta gtatccgcgg tcaccccgaa cctgcaggtg cgctacgtga
     4501 aaccattgtt tgtcggtatc ttcgcgctca ccgcgtgtac ggccgcggcc gccgccacgc
     4561 cgcccagctt cagcacccag cgcttgtccc gggaggtgaa aaccctgtcc tcggatgcgt
     4621 tcgagggccg cggtccagcc actccggcgg aggacaagac catcgattac gtggtcgcac
     4681 gattcaaggc cgcgggtgtg cagccgggcg gcgatctcaa ggacggcaag cgcacctgga
     4741 cgcaggcggt gccgctgctg cgcagcgaca tcgtcggaac gccgtcgctg accctgaccg
     4801 tcgacggcaa ggcccgcaag ctgacccagg gcgaggaaat cgcaattcgc tcgccgctgg
     4861 acggctcgaa atccgtcgac gtcgccaacc tgccgctggt gttcgtcggc tacggcgtcg
     4921 ccgcgccgga acggaactgg gacgactaca agggcgtcga cctgcacggc aagatcgcgg
     4981 tggtgctggt caacgacccc gatttcgaaa ccggccacgg cgagttcggc ggcaaggcca
     5041 tgacctacta cggccgctgg acctacaagt acgaggaagg cgcgcgccgc ggcgcggccg
     5101 gcgtcctggt ggtgcacgaa accgcgccgg cttcgtacgg ctgggcgacg gtgaagaact
     5161 ccaacaccaa cagcatgttc gacatcgtgc gcaagcagcc agccaaggcg catacccagc
     5221 tcgaaggctg gatccagcac gacctcgcgg tgtcgatgtt caagcaggcg gggctcgact
     5281 tcgatgccct gaagaagcag gcgcagacgc gcgacttcaa accggtggcg ttgaagggcg
     5341 aaaccctggg cgcccgcttc aaggtcgatt cgcgagtcat cacctcgcac aacatcgtcg
     5401 cacgcatcga gggcagcaag tatcccgacc agacggtgat ctacagcgcg cactgggacc
     5461 acctcggcgt gggcaagccg gacgccagcg gcgaccgcgt ctacaacggc gcagtcgaca
     5521 acgcgaccgg cacggccgcg ctgatcgaaa tggcccgcgc gttcgcccat ggtccgcggc
     5581 ccgagcgttc ggtcgtgttc ctcaacgtca cggccgagga aaagggcctg ctcggctcgg
     5641 agtactacgc ggccaacccg ctgtacccgc tggccaccac cgccggcgtg ctgaacatgg
     5701 acgcgctcag tccgatgggt cccacccgcg acttcacgat ttccgggacc gccaaactcg
     5761 gcttgctgga tgacctgatc gccgccggca aggccttcgg catgacctac acgcccgatt
     5821 cccatccgga ggccggatac ttcttccgct ccgaccactt ctcgttcgcc aagcgcggcg
     5881 tgccggcggt ttcgttcggt ggcggcgagg acctggtgga tggcggcgtc aaggccggcc
     5941 gcgccgcggc cgccgattac acggcccacc gctaccacca gcccggcgac gagtggcagg
     6001 cgaactggcc gttcaccggc atggcgcggg acctgcaact gctctacacg gtcggcagca
     6061 ggctcgccca ttcgcaggaa tggccgaact ggggcaagga ttccgaattc cgcgccatgc
     6121 gcgacgccag cgaagccgag cgcagttgat gcaacgttgc gccctcccgc agtgcgggag
     6181 ggcgcacccc gagccggcgg acggtcgccc cgagcctgag gattgcaccg tggcaaggtc
     6241 cggtcgcagg gcgcacaatg cccgcgtcca caactcgcag gcggcgacgg aatggggctc
     6301 tactttcccg gtccgcgata ttcgcagcct tcgccgttct gggtgcgctt gacgaccacc
     6361 cgctggagtt gcggcaactc gccgagcttg tcccagacga atgcggcgat gttttccatc
     6421 gtcgggatgc cgaggccctc gacgtcgttg agcaggcgat ggtcgagcgc gtcgcgcgcg
     6481 acctcgatcc gtcgctccag gtcgcccatg tcgatgaccc agccgcgctc ggtgagcggg
     6541 ccgcgcagcc atacctcgac ctggtacgaa tgcccgtgca tgcgccggta gcgcggatca
     6601 cctgccggtc cggcatcgag ctggtgggcc gcgtcgaagc ggaattcctt ccagacggtg
     6661 aagatcttgt cgctcatgga atgcccaggt acttgtgggt ttgcaggctc agccgccagc
     6721 gcgggtgggc cttgcagtaa tcgactgtcg cgcgggtgtt ttcgcgggcg cgcgggccgt
     6781 ccatcggctg caggaagaag tgcccggcgt cgaagccggt gaatcgctcc ggcggcgcgt
     6841 tcgactgcgg atacacgagc ttcagctcgt ccgcgcggcg cagcttcacc ggctgggtgc
     6901 ccttggggct gacggtgatc cagtcgaggc cttccggtgc ctccagggtg ccgttggttt
     6961 cgacggcgca ttcgaacccg cggacgtgca tggcttccag caatgtggtg tcgagttgca
     7021 gcagcggctc gccgccggtg aacaccacga agcggttctg cggcgcgtcg gccggccaca
     7081 gcgaagcgat cttctctgcg accgcggcgg catccggata cttgccgccg ttgtcgccgt
     7141 cggtgcccac gaaatcggtg tcgcagaaat tgcacgcggc agccgcgcgg tcggcctcgc
     7201 ggcccgacca caggttgcac cccgcgaatc ggcagaacac cgccggacgg ccggcgtgga
     7261 agccttcgcc ctgcagggta tggaaaattt ccttgatcgc gtacatgccg gcatcgaatc
     7321 ctgaacccgc tattttgcca gccccgcgtt cggccgtcgc ggtaagctgt ccgctccgtc
     7381 cgtcaggcac ttgccgggaa ccgaaggatg ctgcaacgcg agccgccttc cgcactgcga
     7441 tctttcgtcg gcatgctctg ggcgggcgag ccggccgcgg cctgccggcc cgtgcgcgaa
     7501 cgtgtgttgc ccacggggcg catgcacctg gtgttccgcg cgtcgaccca accgctgcgg
     7561 atcctcggtg agccgcaggc ctcttccggc ggcacgttct gccatgcgat cgtgggcggc
     7621 gcccgcgctt gcgcttacct gcgcgaagtc gccccgggcg gcgcttcggt cggtgccgaa
     7681 ttgtgcgctg gcgccagcat ggcgctgttc ggtgtacccg ccgacgcgct ggcggcccgg
     7741 cacacgcccc tcgaagacct gtggggcgcc gcggccatgc gggcctgcga ccgcattttc
     7801 tcggcgcccg acgcggcgcg ccaactcgat gtattcgcgg gcatcctcgc cgcgcggttg
     7861 ccgcgcgtca gtggactgca tccggcggtg gcgtcggcac tcgcggcatc ccgcgaatgc
     7921 ggggaagtgc acgccatggt cgcggccagc ggctacagcc atcggcactt caccgcggtg
     7981 ttcgaacgtg aaaccggatt gacgccgaag cgcttctgtc gcgtgcaacg cttcggcagg
     8041 ctgctcgcgc atgccgcggc ccgcccggcg ctgtcatgga cggaactggc aatggccggc
     8101 ggctacagcg accagccgca cttcaaccgc gaattccatg ccttcgccgg catcacgccg
     8161 ggcgaatatc gccgtgcagc accgcgctac ccgcaccacg ttccgatcga ttgaatgtgg
     8221 ttcgcgttcc acgcgcggat cgcccggcgc aggtcaaatt ccttcaagca ccggacgcgc
     8281 agccgcacca caatggcgat cgtccattcc gaggaaccgc catggccatc cacgaagtat
     8341 tcgcctatct caccgtcgac ggcgcggccg aggccatcga cttctacgta cgcgcgttcg
     8401 gcgcgcgcga gaagttccgc ctcgccgaac cgggtggacg catcggccat gccgaactcg
     8461 acttcggcgg caccgtcgtc atgctggccg atacattccc cgaattcggc ctgcgcgatc
     8521 cgaaggcgct gggcggaacg ccggcttcga tccacctgca cgtggacgat gccgacgcga
     8581 tgatccggcg cgcggtcgac gccggcgcga ccctcgagcg cgagcccacc gaccatttct
     8641 acggtgaacg cgccggatcg gttttcgatc cgttcggcca ccgctggctg gtcggacaca
     8701 gcatcgagga cgtgccgccg gaggagatgc aacgacgcta tacggcgatg gtgcaggggc
     8761 aataagcccg ccacgggaag agaatcgcgg catgcaaatc cgtacggccc gcgtcgccga
     8821 cgccgaagcg atcgcaaggg gggaagccga aaccgcgcgg atgccgggcc tgctggtcgg
     8881 tcggcccggc gaaatccccc tggatgccta ccgcgccaag atcgcgaagc tggaaagcca
     8941 ggggcgctac atcgtggccg agcacgatgg cgccgtcatc ggccatgcgt ttctcgaccc
     9001 gatgcccatg cgcgccaacg cgcacgtcca cctgctcacg atcgtggtgc atccggccca
     9061 catcggacgc gggatcggcc gggcgatgtt gcgcgacctg ctcgaatggg cgcagggcga
     9121 ccgccgcgta ggcaaggtcg agctgaacgt ccgtgccggc aacctgcggg cgcagaacct
     9181 gtaccgcagc atgggcttcg tcgaggaagc ccgtttccgt gggcgcgtgc agcgagccga
     9241 cggacaactc gaggatgatg tcggcatggc atggttcccc gaacgctagc ctgccgccgg
     9301 catcggtgac aaactgacgg ccgacgagcc agcgggagcg cgcatggcgg accgcgtcga
     9361 agacgcccgg gcactcgata ccctgcggca gatggccgcg ctgcaggcgc gcgtgtgcta
     9421 catgaacgtc gatcgcacgc tggcgacctg gacgcgcagc gcattgacgc tgatcgtgtt
     9481 cggcacggtg gtcgatcgct atggcctgct gctgttgcgc gaccactttc cgcacgtcgg
     9541 cacccggctg gcccccaacc cggtatcgag cgccggcgga ctgctgctgg tcgcgatcgg
     9601 cgtattcatc gcgatcgcgg cggcgatccg ccaccagcga tacaaggcga cgtggcaccg
     9661 cgcattcggc cgtgacgaaa cgttcggccc gtggctggca tttcctttcg ccgtgggagt
     9721 ggcgatgctc ggtgtcacga tcctcgcgtt gctgctggtg ctggtgcaat gaggccgtcc
     9781 cgcgtttccc gcgcgcccgc ggagtcctcg catggccgat gacgcccaca acctgcaggt
     9841 cgaggccgca cgcgagctgg tggagctctc gctccagcgc tcgtacatga ataccgagcg
     9901 cacgctggcg gtatggacgc gcacggcgct gtcgctgatg atcttcggga tcgcggtgga
     9961 ccgcttcggg ctgctgatgc ggcatccgcc gtggcatgta ttggacaccc gccaattgct
    10021 gccggatccg ctttccaccc tgggcggggt ggtgctggtc gcgatgggcg tcgcgatcct
    10081 cctggcagcc ggattccgct tcctcgccta cggcttgaag tggtaccggc gcaacccgcg
    10141 caaggtgtgg ttccacaccc cgtggctggc cttctcgttc gcgatgctga tggcggcgtt
    10201 cggcatcgcg ctgctcgcgg tgttgctgat cttccagcag taggacggac ggcgcccgcc
    10261 gttgggcgac cgagtgcact tgcggcgcgg aggcccctac atcctgacag gtcccgcctt
    10321 gtggcacttg tcgcgcgcgg cgcgcgcggt ggtcacgtcc gcctggcggt cctgcgcttc
    10381 ggcgacttcc aggatcgtct gccagttgct cgcgcacagg ctgccgacct tggggcctag
    10441 catgaacgag tgccgtgcgt cggcctcggc cgcggggtag ttgcgcaaca ggatctcgac
    10501 ctcggcgcgg aactgcagga tgtccggcgc gtcgggcgac agcttcagcg cggcgtcgat
    10561 cctgtcgagg gcatcctggt agttgccggc gttctcggcc gcatgcgcct gccccaggaa
    10621 gccgtcgacc gccggatcgc gcagcggcgc aacctgcacc accgacttgt cggcggcgcc
    10681 cgcggcgcgg atcgccgcca ccatcgccgt cggtgaattg cgctccgatg ccggcggcgg
    10741 cagtggcgga ttcgagcatg ccgcaagcag caacgcacct gcggcgacgg ccgtggccgc
    10801 gacgcggccg gatttcgaca tcatctgttc cactccaggt ttcagcgcca ggcccgtgcg
    10861 ccggccgacg gctgcgacga tccgtcgccg gaatcgccgc tgctgaaaat gtgttccagg
    10921 cgctgcaggt agcaatgatt ctgcacctgc ggcaggtagc cgtcgatgaa aggatactcg
    10981 cgtgcgccgt cgcactgggg cagcgtgcct tgtccggtgg acggatcaat ccacgcgtac
    11041 tggatcccgt cgccgaacga ggtatccagc ggcgtgctcg gcatcttctc gaacagtttc
    11101 atccacacct tcagcgcgcc ggtggcaccc cacaggtggg tcggcttgtt gtcgtcgcgg
    11161 cccatccacg cgaccgccag gcgatcgccg gtgaagccgg aataccacga atcgcgctga
    11221 tggtcggagg tgccggtctt gccggcggcg tgcaggtcgg cgagttcggg atggtcgccg
    11281 atcgcggcgg cggtgccgta ctgggtgact tgctgcatca tccagcggac caggcgctcg
    11341 gcctgccggt actggccctt gccgggcttc acctcgtact gcttcagaac gtgtccgctg
    11401 cgatcgagca cgccgcggag ggtcagcagc ggcagcgcgt gcccgtcgcc ggcgaagaac
    11461 tcgtacaact gcgcgagctg gaacggcgac atgtcgatgg cgcccagcag cagcgacggg
    11521 ttgggctgca cgccctcgag cccgaacgaa cgcaggaacg cggcgacccg atcgacgccg
    11581 agctggaggc caaggtgcac gctcgcgagg ttgtaggact tggcgagcgc ctcgaccatc
    11641 ggcacttcgc cgtgcgaccg gttgtcgtcg ttgtgaggcg tccacgactt gccgttgcgc
    11701 agcttcaacg agatcggcga atcatccagc agcgacgcca cgttccagcg ggacgggtcg
    11761 gtcagcgcca tcaggtagta gaacggcttc aggctggagc cgaccggccg ccgcgcgtcg
    11821 taggcgcggt tgaaaccgtg caggtccgat tcccgtccgc cgaccattgc cagcacgctg
    11881 ccggtggacg gctcgaccac caccaccgcg ccctcgagct ggttgccgcg cttgccgagt
    11941 cccttgatgg cttcgtccag cgactgctcg gcgtacacct gcgcggcagg atcgagcgtg
    12001 gtgtacacgg aaagcccgcc gttgcgaagc tgctgctcgc cgaagtcgcg ggcgatctgc
    12061 tcgcgcacca cctgcatgaa tgccgggaag cgatcgacca ccagctgcgg cttcggggtc
    12121 acgtccagcg gcgccgattt cgcggcctgc cactgcgcat cgttcaacag cccggtggca
    12181 tggaaggcag cgagcgcgac attgcggcgg gcgagcgcgc gctgcggatt gcggcggggt
    12241 tcgtaatagc tcgggccctg caccattccc accagcagcg cgatttcctg cggccgcagc
    12301 gtctgcagcg gccgcccgaa gtagtaccag ctcgcggcgc cgaaaccgcg caccacctgg
    12361 ttgccctgct ggccgaggaa cacctcgttg cagtacgcat tgaggatttc gcccttgctg
    12421 aagtgcgcgt ccagcaggat cgacagcagc gcctcgttga acttgcgaac cagtgtctgg
    12481 ctgcggtcga ggaacaggtt gcgcaccagt tgctgggtga tcgtcgaacc gccttgcacc
    12541 acccggccgg cgcgcaggtc ggcgagtgcc gcacgcacta tcgaggacag gtcgatgcca
    12601 tggttgttct tgaagccgcg gtcctcgacc gcctgcacgc cctgcaccag cagcggcggc
    12661 atttccgaca gcggcaggat gatgcgctcc tcctgccggg cgccgtacat cgtcgcgatc
    12721 cgcgcggggt cgaggtgggt cttgtggagc ggcttgttcg ccgtgatgtc gaacaccgac
    12781 tggatgcggc ccgaaccgag ccgcacgcgt atccggcgcg gcaattcgcc gccgaccgga
    12841 tccatgtaac cgcgcgaggc gatcacgaag gtgccgccgt ccgcgctcca ggtacccggc
    12901 acccgcgcgc tggatggcgc ctcggtgtag ccggccatgc cgagttccag cttcagggtt
    12961 gccttgtcca tcggcacccc ggcggccagc agttgcggac gcgcgtacac ccgcgtcggc
    13021 tgcgtgaagt cgatttcact cacgcgggtc ttcacgcgat ggtcgaggac cagcgtgtag
    13081 ggaaccacga agccgacggc gagtcctatg ccgatcagga tgggccagcg tgcgaagggc
    13141 cagacccggc gcacgcgctc gaaccaggac aaggtcttgt caacgatcca gcagggtgag
    13201 cgcgaagtct agcggatcga cccggcatcc cggcgtgatg ccgctggcgg attcagcggc
    13261 gattgtcgtt ccgcccccgc cgccgcgccg cgtcaccaac ggcgcgtcac caggaagctc
    13321 gcgccgcagc cctggtcgac ccagatgccc gcgcggttcc agccccagct ccggccttcg
    13381 atgcacgccg agtcggacaa ctggcgctgc aggcgcacgt ggccgcgcgc gccgacgtcc
    13441 acctggcaga agttgcggcg gtattgcgga ctgccgcagg agacgacgaa gtcacggtcc
    13501 cagcccggcc ccgggcgcca gccgcccacg gccgcgaact cgccgccgca gccttcgttg
    13561 acccagatgc tgccacgatc gaatccccag gttcgtccct ccacgcaggc cgaatcggag
    13621 gtctggcgga ccagacgcgc accgcgccaa cccggcggca ccgggcaccg cgccaggtgg
    13681 tattggggac tgccgcaggc cacgacctgc cccggattcc cggtgtaacc gggcggcggc
    13741 ggacggttgc tgtaggtgac ctgcgcggtc gcggcggccg cgaacatcca cgcgaagcct
    13801 gcgagaacga ccggaacgct gcgcatgtcg ctgccctccc gtgaccgtac cgcaccagtg
    13861 ttgacgctcc aggctgaatg gagactggcg ggccccggcc acatgcgggc ggcaggtagg
    13921 tccgccctgg gacgaggccg ccgtcagtcc ttcgaagccg ccgatgtccc cgcagccttg
    13981 ggctcggccg cgttggcggt cttcgcgtcg ccgccgccgg aggtcggctt ctcgccggc
//
LOCUS       DASSNH010000209         5330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1153177, whole genome shotgun sequence.
ACCESSION   DASSNH010000209 DASSNH010000000
VERSION     DASSNH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153177"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..3255
                     /locus_tag="VFK00_07625"
     CDS             394..3255
                     /locus_tag="VFK00_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7592855.1"
                     /translation="METFAVPADLLTQPVLLVSTGGLIKACNVAFAASIGMTPEALQG
                     RDLASLVADADRDLLEYLHACADGQASPARLTFSSGGATETYSARGIAYGLSEDPASR
                     PILLLLKPLHGRADHPPPAEAAGDAAQPGRSAVSPGTGSKLLDVTLASIGDGVIVTDA
                     SGRVTFLNPVAEQLTGWSSAEARNAPFAEVFRIVNEYTGAPVDHPVAKVINSGGIVGL
                     ANHTMLIGRDGRRIPIDDSGAPIRHNDALAGIVVVFRDVTERRRAEHERGRLAAIVEG
                     SDDAIASKTLDGIVTSWNPAAARLLGYRPEEIIGKSVTLIIPPERQAEEDAILGQLRR
                     GERVDHFETVRLAKDGTRLDVSLTISPIRDPTGAIVGASKILRDITARKRSEERLREE
                     TQVRELLGEAARVVVTAQLDLERVVQGITDIAREITGAAFGAFFYNVQDERGESYRLY
                     TISGASIEAFSRFPQPRNTPVFGPAFRGEANVRSGDITADPRYGHNPPYHGMPEGHLP
                     VRSYLAVPVKAASGEVLGALFFGHPEPDVFTERAEQLVVAVAAQAAIAVESARAHQAL
                     KQEIDHRKQAEQALHQAGRQKDEFLAMLAHELRNPLAPIRNATELLSRMLADDSNAQA
                     ALRMIERQGLQLSRLVDDLLDVSRITTGKIQLEHKIIDLAAVVTQALETAEPQVREKR
                     HRVSVTTFHEPLYVEGDFARLVQCVGNVLANAIKYTEPGGEIWVRTRGDSSSAFIEIS
                     DTGAGIPPELLPQVFELFVQSDRTLDRAQGGLGVGLAVVKRLVEMHNGEVTARSEGLW
                     RGSTFEIRLPRVASPAPAAAAEARFKPESRRVLIVDDNRDAADSLALLLKFEGHETHV
                     VYSGEEALASMASFKPDIAFLDIGLPGMDGYELARQIRKMPSLKGLRLVALTGYGQVE
                     DQQRAHAAGFDSHLVKPVSLSALEHAISGVLSRPMNPGE"
     gene            complement(3423..3497)
                     /locus_tag="VFK00_07630"
     tRNA            complement(3423..3497)
                     /locus_tag="VFK00_07630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3463..3465),aa:Val,seq:cac)
     gene            3595..3915
                     /locus_tag="VFK00_07635"
     CDS             3595..3915
                     /locus_tag="VFK00_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HET7592856.1"
                     /translation="MEHILHAITNFQLTPWKVVGFLGTAVFASRWFVQLYATRKQRKV
                     VMPMLFWYMSVCGSVLLLAYFIFGKNDSVGIISNLFPMFVALYNVSVHMRHRQQEKAG
                     SAAT"
     gene            4072..4335
                     /locus_tag="VFK00_07640"
     CDS             4072..4335
                     /locus_tag="VFK00_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592857.1"
                     /translation="MNAQLSAREASPVSLGDWIITLIVLAIPIVGIVMLFVWAFSGST
                     PPSKQNYCRAALILAAVVFALWLVFFFTIGASLLHTAMGGATL"
     gene            complement(4376..>5330)
                     /locus_tag="VFK00_07645"
     CDS             complement(4376..>5330)
                     /locus_tag="VFK00_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET7592858.1"
                     /translation="ARANGSKFDWKNYTPPVPNKPGVTVIDDIPIPELLKVTDWTPFF
                     QAWELHGHYPAILNDPVVGPQATELFNDAQEILKRVIEEKWLRAKAVIGFWPVSRVGD
                     DLELVNGDERVTLHHLRQQADKPAERPNLCLADFIAPKETGVQDWIGGFAVTAGLGIE
                     EHLERFRKDNDDYSAIILKALADRMAEALAEWTHREVRTRYWGYAPDEAMSNEDLIAE
                     KWRGIRPAPGYPACPDHTEKATLFRLLDATNNASIELTEGFSMYPAAAVSGWYFSHPD
                     SQYFVVGKLTKDQVEDYAERKGWTLAEAERWLSANLDYDPD"
BASE COUNT          928 a         1684 c         1740 g          978 t
ORIGIN      
        1 tgcgccgacc agcgaaatac cgggtgattc cgggcattcg cccgacatcg gggttccggt
       61 tttcgaacgg caatccaccc gctcgatccg ccctgcccat ggatattcgc gcgggtggca
      121 ttggccgctg atccttcatg gcacttgtgc tagccttccg caactgtcaa cggtgcgtca
      181 tgcagccacg ctcctccgtt cccgaatgag ctgcaaccct ttcgaccggg tgaacgggaa
      241 gtcttcacgt tttccggcgg ctgcatcgga tgcgccaggt tcgatccgac ggcaaccgtc
      301 gcgtctgtcg gatcccgcga tcgcagagga gcggggcgtc ggggaaacat caccgaaggt
      361 aggattttct gcatcgctcg tacgcgcatg cccatggaaa ccttcgctgt cccagccgat
      421 ctcttgaccc agccggtact gctcgtttcg acgggcgggt tgatcaaggc atgcaacgtc
      481 gcctttgcgg ccagcatcgg aatgaccccg gaagcattgc agggccgcga cctggcctcg
      541 ctggtcgcag atgccgaccg cgacctgctc gagtacttgc acgcctgcgc agatggccag
      601 gcctcgccgg cgagactgac cttcagcagc gggggtgcca ccgagactta cagcgcgcga
      661 ggcatcgcgt acgggctgtc ggaggatcct gcctcgcgac cgatccttct tctgctgaag
      721 ccgctgcatg ggcgggcgga tcatccgccg ccggccgagg cagccgggga tgccgcgcaa
      781 ccgggtcgca gcgcagtctc tccgggaacc gggagcaagc tactcgacgt aaccctcgcg
      841 agcatcggcg acggcgtcat cgtcacggac gcctcgggcc gcgtgacatt tctcaatccg
      901 gtcgccgaac aactcaccgg ctggtcctcg gcggaggcga gaaatgcgcc gttcgcggaa
      961 gtcttccgta tcgtcaatga atataccggt gccccggtcg accacccggt ggcgaaggtc
     1021 atcaactccg gtggaatcgt gggtcttgca aatcacacga tgctcatcgg ccgggacggc
     1081 aggcgaattc cgatcgacga cagcggcgcg cccatccgcc acaacgatgc actggccgga
     1141 atcgtcgttg tctttcgcga cgtgaccgaa cgcaggcgcg cagagcatga gcggggcagg
     1201 ctcgcggcca tcgtggaggg gtcggacgat gccatcgcga gcaagacgct ggacggcatc
     1261 gtgacatcct ggaatcccgc cgcggcgcgc cttctcggat accggcctga agagatcatc
     1321 ggcaagtccg tcacgctcat catcccgccc gagcggcagg ccgaagagga cgcgatcctc
     1381 ggtcagctgc gacgcggaga gcgcgtggac cacttcgaaa ccgtgcgcct cgccaaggac
     1441 ggaacccgcc tcgatgtttc gctgaccatc tccccgatcc gggaccccac cggtgcaatc
     1501 gtcggcgcct cgaagatctt gcgggatatc acggcccgca aacgttccga ggagcgcctg
     1561 cgggaagaaa cccaggtgcg ggaactgctc ggggaggccg cccgggtcgt ggtcactgct
     1621 caactggacc tcgaacgcgt cgtacagggc atcaccgaca tcgcgaggga gatcaccggc
     1681 gccgcgttcg gtgcattctt ctacaacgtc caggatgaac gcggcgaatc ataccggctg
     1741 tacacgattt ccggggcgtc gatcgaggct ttttccaggt ttccccagcc ccgcaacacg
     1801 ccggtgttcg ggccggcgtt ccgcggcgag gccaacgtgc gcagcggcga catcacggcc
     1861 gatccgcgct acggccacaa cccgccctac cacggcatgc ccgagggaca cttgcctgtc
     1921 cgaagctatc tcgcagtgcc cgtgaaggcg gcttcggggg aggttctcgg ggcactcttc
     1981 ttcgggcatc cggaaccgga tgtattcacc gagcgggccg agcaactcgt ggtcgcggtt
     2041 gccgctcagg cggccatcgc agtcgagagt gcccgcgcgc atcaagcctt gaagcaggaa
     2101 atcgatcatc gcaagcaggc ggagcaggcg ctccaccagg ccggccggca aaaggacgag
     2161 tttctcgcca tgctcgcgca cgaactcagg aatccgctgg ctccgatccg gaatgcgacg
     2221 gaactgctgt cccgcatgct ggcggacgac agcaatgccc aggcggcgct tcgcatgatc
     2281 gagcgccagg gcctgcagct gtcgcgactt gtcgacgact tgctggatgt ttcacgcatc
     2341 acgaccggga agatccagct cgaacacaag atcatcgacc tcgccgcggt cgtcacccag
     2401 gcgctggaaa ccgcagagcc ccaggtgcgc gagaagcggc acagggtctc cgtcaccact
     2461 tttcacgaac cgttgtacgt ggagggcgac ttcgcgaggc tggttcaatg cgtcggcaac
     2521 gttctcgcga atgccatcaa atacacggaa cccggcggcg agatatgggt tcgaacccgc
     2581 ggcgacagca gcagcgcctt catcgaaatt tcggacacgg gcgcgggaat tccgccggag
     2641 ctgcttccgc aggtcttcga gctgtttgtg cagagcgacc gcacgctcga ccgtgcgcaa
     2701 ggcgggcttg gcgtcggact ggcggtggtc aagcggctgg tcgaaatgca caacggtgag
     2761 gtcaccgcgc gcagcgaagg actgtggcgg ggatcgacgt tcgagatacg gctgccccgg
     2821 gttgcgagcc cggctccggc ggccgccgcc gaggcgcggt tcaaaccaga gtcgcgacgc
     2881 gtgttgatcg tcgacgacaa cagggatgca gcggattcgc tcgcactgct gctcaagttc
     2941 gagggccacg aaacacatgt ggtgtacagc ggcgaagagg cgctcgcgag catggcgtca
     3001 ttcaagccgg acattgcctt cctcgacatc ggactgcccg ggatggacgg atatgaattg
     3061 gcgcggcaga tacgcaagat gccgtcgttg aagggcttgc ggctggttgc gttgaccggg
     3121 tatgggcagg tggaagatca acagcgtgcc cacgctgccg gttttgacag tcacctggtc
     3181 aagccagtca gcctgtcggc gctggagcac gccatcagcg gtgtcctgag ccggccgatg
     3241 aatcccgggg aatgaccgga tcgcatcgcc tcgagccggc gcgaattgct gcgtccacac
     3301 cttcgcgccc cagcaggaag aagtcctgat gctttcgtct cgactggctt gtccggtgct
     3361 gcgacacccg gatgcagaaa cggcccgact cgaggggcag gccgttcgca aatactcgat
     3421 gctggtgggc ggtgcaggga tcgaacctgc gacacctgcc gtgtgaaggc agtgctctac
     3481 cgctgagcta accgcccggc gcctgatgcg aacgcgcaag tctacgggcg cagccggatt
     3541 cggtcaatcg gcggttgcta caattcgcgc acctacagga agccggactc cgccatggaa
     3601 cacattctgc acgccatcac caatttccag ctcacgccct ggaaggtggt cggtttcctc
     3661 ggtaccgccg tcttcgcgtc gcgctggttc gtgcagcttt atgcgacgcg gaagcagcgc
     3721 aaggtcgtga tgccgatgtt gttctggtac atgtcggtgt gcggcagcgt gctgctgctg
     3781 gcctatttca tcttcggcaa gaacgattcg gtgggcatca tctcgaatct gttcccgatg
     3841 ttcgtggcgc tttacaacgt cagcgtgcac atgcgccatc gccagcagga aaaagcgggc
     3901 agcgccgcga cctgaccgct gccgcgggca catgcaagcg cggcaatggt gggctaggat
     3961 cgggcggccg atcaacgcgg cacggggact gacaacgatt ggctgccggc gccgtcgcat
     4021 cgggcggcgg tttgccgggg gcatccgcca tcacctgagc aggagagggg catgaacgca
     4081 cagctttccg cgcgtgaagc gtcgccggtt tctctgggcg actggatcat caccttgatc
     4141 gtgttggcga ttccgatcgt cggaatcgtg atgttgttcg tatgggcgtt ttccggcagc
     4201 acgcctccca gcaagcagaa ttactgccgg gctgccttga tactggccgc ggtggtgttc
     4261 gcgctgtggc tggtgttctt tttcacgatc ggcgcctcgc tcctccacac ggccatgggc
     4321 ggcgcgacgc tttgagttcg gcggcgccgc gcacgtcctt gcgcggcgcc gccgttcaat
     4381 ccgggtcgta gtcgaggttg gccgaaagcc agcgctcggc ctcggccagc gtccagcctt
     4441 tgcgttcggc gtagtcctcg acctggtcct tcgtgagctt gccgaccacg aagtactggc
     4501 tgtcggggtg gctgaaatac cagcccgaaa ccgcggcggc gggatacatc gagaagcctt
     4561 cggtgagttc gatgctggcg ttgttggtcg catccagcag gcggaacagc gtggccttct
     4621 cggtgtggtc ggggcaggcg ggatagccgg gtgccgggcg gatgccacgc cacttctcgg
     4681 cgatcaggtc ctcgttgctc atggcctcat cgggggcgta gccccagtac ctggtgcgca
     4741 cttcgcgatg cgtccactcc gcgagtgctt cggccatccg gtcggcgagg gccttcagga
     4801 tgatggccga gtagtcatcg ttgtccttcc tgaagcgttc gagatgttcc tcgatgccga
     4861 ggcccgcggt gaccgcgaag ccgccgatcc agtcctggac accggtttcc ttcggcgcga
     4921 tgaaatcggc aagacacagg ttggggcgct cggccggttt gtcggcctgc tggcggagat
     4981 ggtgcagcgt tacgcgttca tccccgttga ccagttcgag gtcatcgccc acgcgcgaca
     5041 ccggccagaa cccgatcacc gccttggcac gcagccattt ttcttcgatg acccgcttca
     5101 ggatttcctg tgcgtcgttg aaaagctcgg tcgcctgcgg accgaccacc ggatcgttca
     5161 ggatcgcggg gtagtggccg tgcagttccc acgcctggaa gaacggcgtc cagtcggtca
     5221 ccttcaggag ttcggggatg ggaatgtcgt cgatcaccgt gacgccgggc ttgttcggca
     5281 cgggcggcgt gtagtttttc caatcgaact tcgagccgtt cgcgcgcgcg
//
LOCUS       DASSNH010000210         9529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1438538, whole genome shotgun sequence.
ACCESSION   DASSNH010000210 DASSNH010000000
VERSION     DASSNH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438538"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFK00_07650"
     CDS             complement(<1..599)
                     /locus_tag="VFK00_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7592859.1"
                     /translation="MSVPASRVSQHASLHPQPFLAADVGGTHARVALLRAAAGGKREI
                     EVVAYRKFACADFAGLPELLQAFVARDATMPVAACVIACAGQVIDDVIVNDNLAWRVS
                     LSQLRDALAFEDVALLNDFEALGYALDDVRDSDSLLLCGPDIHPEGPALVVGPGTGLG
                     AALRLPGAGHAQVLATEAGQMDFAPGTAREREVLARLAPG"
     gene            997..2310
                     /locus_tag="VFK00_07655"
     CDS             997..2310
                     /locus_tag="VFK00_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HET7592860.1"
                     /translation="MTTPASRSNAPSYVMSMVIIGVLFFIFGFVTWLNSSLILFVKVG
                     FDLDNVSAFLIPLAFYLSYFFLALPSAVVLKRTGMKKGMALGLFVMAVGSVLFGQFMA
                     MYWYAGALIGVFVIGAGLALLQTASNPYVSILGPIESGAKRVAIMGICNKFAGGVAPY
                     VFGALLLSGVDAYADKIKAAASPQARTALQHAFVDRAFAPYMVMAIVLVILAIWVVFS
                     SLPEIKPEANRAVADTRQPTRSIFSFPHLWLGVVCLFIYVGMEVMAGDGIVVYGQASG
                     LALGVVKHLTTFTMIGMLVGYLVGVALTPRFISQQRYLAMSAVLGALFAFGAWATHGP
                     LSVAFVAALGFANAMMWPAIFPLAIRGLGKWTEFGSALLIMGIAGGALIPQLFVHLEE
                     IIDFQLAFLLVMVPGYAYILFYGLHGYRAGQYNQARAIDSAIAAP"
     gene            2363..3385
                     /locus_tag="VFK00_07660"
     CDS             2363..3385
                     /locus_tag="VFK00_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7592861.1"
                     /translation="MATATIRDVAKQAKVSLKTVSRVINREGSVREETRERVLRAVEA
                     LDYRPDLSARSLRSAKAYAIGLVYDNPNPYYIIAVQQGALAACREMGFGLQIHPCNSR
                     GPTLAEELRDLARRSRLAGLVLAPPMSEHVELLRELAAYNVPLVRIISAAEDPRDGFP
                     CVFVDDRDAAYAITAYLIQLGHVRIGFLWGGKQHRSSPERYKGYEQALQDYGVPLSDE
                     LVLEGDYSFDDGFRGARRLLGLAEPPTAIFGSNDEIAAGVLAAARAGGLHVPYDLSVA
                     GFEDSPFSRHSWPSLTTSRQRTEAIAEHATRLLIAQIHGDDVENEGFSPELVVRGSTA
                     PPRPRT"
     gene            3406..4461
                     /locus_tag="VFK00_07665"
     CDS             3406..4461
                     /locus_tag="VFK00_07665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7592862.1"
                     /translation="MSATPNRDLRPESTFLYREAAEAADAVARQLEHNDACVHRLAQA
                     LRDSPPPFVVTCARGSSDHAATYAKYVIETQTGCATASASPSVESVYAVPLRLRGALY
                     IVISQSGASPDLLRSAEAAREAGARVVALVNAEGSPLATLADTVIPLRAGIEQSVAAS
                     KSFIGSLAAILHLVAHWRADAALLRGLHDLPEQLRRAFALDWSPLVDGLAGMHDLFVL
                     GRGYGLGAAQEAALKLKETCALHAEAFSSAEVRHGPMELVGPDLPVLLFAQADDALAG
                     VLATAEDFRRRGATVWTAAPGASGMGALPIVPAVQPMCAPILAIQSFYRAANALALRR
                     GRDPDRPSNLAKVTETV"
     gene            4461..5603
                     /gene="nagA"
                     /locus_tag="VFK00_07670"
     CDS             4461..5603
                     /gene="nagA"
                     /locus_tag="VFK00_07670"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7592863.1"
                     /translation="MTTALVNARVLTAQGWRDDVAVVVDGGRILELVASDGIAFGTEV
                     RDLGGRMLLPGFIDCQVNGGGGVLFNDQPTVEGIRAIGEAHRRFGTTGFLPTLISDTS
                     EKMQAAIAAVGAAIGEGVPGVLGIHLEGPFLSREKRGVHAEQFLHAPDSVELQTAASL
                     QRGLTLLTLAPERVDPEAIRGLAERGVIIAAGHSNADHDTVRAALDAGVRGFTHLFNA
                     MSPLASRAPGMVGAALDHAESWCGVIADGHHVHPAAIRIALKAKPRGKIFFVTDAMPP
                     VGADDPSFTLGGETITARDGICQTANGTLAGSALSMIEAIRNAVTMLGVPLEEAARMA
                     STYPANFLGLGDTHGRIAPGCRANFTVIDAELRVTETWIDGVPQAN"
     gene            complement(5898..7085)
                     /locus_tag="VFK00_07675"
     CDS             complement(5898..7085)
                     /locus_tag="VFK00_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7592864.1"
                     /translation="MKRNGLFAVIALALGTAGVMSVAHAQDTGMATESTGSFTNPDYS
                     NWYIVPRIGVVVPDSDRRVQTDPVGGLGVGFWITPNFTLDVEATGDNADFKDDSPRPG
                     KQWETVGLDVAGRWYFMDPTSQWRPFVMAGVGFWRHAAVSEELAQAGGECGCLGPHSN
                     SGWGPGATVGVGVLYNVSDRIAVRGEIAARYYRDVNSAQLAGLAGPGLPNTYGPKQWL
                     DTMATVGLVFNMGHPAPPPPPPAAPPPPPAAPPPPPPQQNVVIDLRGVNFKFDRPKKG
                     ESDIGPTLKPPQADSIAILDQAVDTLNRYPQVQIEIDGYTDSVGTDQYNQGLSERRAN
                     IVDQYLTSHGIDSSRITAVKGFGENDPIDTNKTAAGRQRNRRVEFKVEGQGMNQDQQQ
                     QQQ"
     gene            complement(7270..8733)
                     /locus_tag="VFK00_07680"
     CDS             complement(7270..8733)
                     /locus_tag="VFK00_07680"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HET7592865.1"
                     /translation="MSHADIRSAARPAPDQPLLDIADYVLDYRVESQEAWETARYMLM
                     DALACAMLAMKFPQCVRHLAPIVSGATLAGGARVPGTDLELDPAQAAFCIGTQIRWLD
                     FNDTWLAAEWGHPSDNLGAVLAVADWLSRRNMRNGDKPLTIRDVLGWAIKAHEIQGCY
                     ALKNSFNRAGMDHVILVRLASTAVATAMLGGDRGQVVTAVSNSWIDNGALRTYRHAPN
                     TGPRKSWAAGDACRRAVIHALDALTTGEPSYPSALSAKTWGFYDVAFGGKPFEFERPF
                     GSYVMENILFKISYPAEFHAQTAVECAMKLHPQVKDRIGDIERIEIETQEAGARIIDK
                     TGPLANYADRDHCIQYMVAVPLIFGRLVADDYSDVVAADPRIDALRGRMSVRENPQFT
                     RDYFDPDKRYIGNSVQVFFKDGSKTAKVSIDYPIGHRRRRAEGIPVLLGKFRGALSAF
                     LPEAQAAEIIGLCEKPEDMLDTPVPAFMDQFAMQARA"
     gene            8991..>9529
                     /locus_tag="VFK00_07685"
     CDS             8991..>9529
                     /locus_tag="VFK00_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7592866.1"
                     /translation="MPPIEALLHHPLDSAFWSGLASIIVIDLVLAGDNAVVIALAARH
                     LPKNHQFRAIFWGTVGAIVVRVAMTAGVIWLLRIPALMLVGGLVLIPIAFRLLKQDEQ
                     DDDPHLRHKVTGFWSAMGTIVVADALMGLDNVLAIAGASNGHLGLVALGLLISVPLVV
                     WGSTLLLKLIERFPVIIYIG"
BASE COUNT         1557 a         3133 c         3156 g         1683 t
ORIGIN      
        1 ccgggcgcga ggcgcgcgag cacctcgcgt tcgcgcgcgg tgcctggcgc gaaatccatc
       61 tggccggctt cggtggcgag cacctgcgcg tggcccgccc ccggcaggcg cagcgccgcg
      121 ccgagaccgg tacccgggcc cactaccagc gccggaccct ccggatggat gtccggcccg
      181 cacagcagca ggctgtcgga atccctcacg tcgtccagcg cgtagccgag cgcctcgaag
      241 tcgttgagca gcgccacgtc ctcgaacgcg agcgcgtcgc gcaactgcga aaggctgacc
      301 cgccacgcaa gattgtcgtt gacgatgacg tcatcgatca cctggcccgc gcaggcaatg
      361 acgcaggccg ccaccggcat cgtcgcgtcg cgcgcgacga aggcctggag cagttccggc
      421 aacccggcga aatccgcgca cgcgaacttg cggtaggcca cgacctcgat ttcgcgcttg
      481 ccacccgcgg ccgcgcgcaa cagcgcgacc cgcgcatggg tgccgcccac gtccgcggcc
      541 aggaatggct gggggtggag gctcgcgtgc tggctcacac ggctggcggg cacactcatc
      601 ctgcgctcgg acaatggaat gggcccagtg tggtcaagga tgacaacgtt gtcaatttgc
      661 gctgcagcaa tgcagagaag catttgtcga tagcggaaaa gccgcggcgt ggcgcccgaa
      721 tgcaagcatg tataaggcag tgccagggtt atcgatgcgg cgttgcatca aaacaaggcg
      781 cggctgctgc cccgcgatta cgagcttcaa accaatcgcc tacccaaatg cgagtcatga
      841 tcatgacaac gttggtcaag ccatagtacg ctgtgccccg aagtggattg gcgcgcatgc
      901 gatggatccc gacgcagcgg atgcatggca tggtgaaatg cacggcggag ttttcgatcc
      961 ggctacccac ccatcggcaa tcgagcaggc gcaggcatga ctaccccggc aagtcggtcg
     1021 aacgcaccct cctacgtcat gtcgatggtg atcatcggcg tgttgttctt catcttcgga
     1081 ttcgtcacct ggctcaacag ttcgttgatc ctgttcgtca aggtcggatt cgacctcgac
     1141 aacgtcagcg ccttcctcat tccgctggcg ttctatctct cctacttttt cctcgcgctg
     1201 ccttcggcgg tggtgctcaa gcgcaccggc atgaagaagg gcatggcgct cggcttgttc
     1261 gtgatggccg tgggctcggt gctgttcggc cagttcatgg ccatgtactg gtacgccggc
     1321 gcgttgatcg gcgtgttcgt gataggcgcg ggcctggcgc tgttgcagac ggcttccaat
     1381 ccctacgtca gcatcctcgg cccgatcgag agcggggcca agcgggtcgc gatcatgggc
     1441 atctgcaaca agttcgccgg cggcgtcgcg ccttacgtat tcggtgcctt gctgctcagc
     1501 ggcgtcgacg cctacgcgga caagatcaag gccgccgcgt cgccgcaggc aaggacagcg
     1561 ctgcagcacg cattcgtgga tcgcgcgttc gcgccctaca tggtcatggc aatcgtgttg
     1621 gtgatcctgg cgatatgggt cgtgttttca tcgttgccgg agatcaagcc ggaggccaac
     1681 cgcgccgtcg cggacacccg gcaacccacg cgcagcatct tcagctttcc gcacctgtgg
     1741 ctgggcgtgg tctgtctgtt catctacgtg ggcatggaag tcatggcggg cgacggcatc
     1801 gtggtctacg gccaggcgtc ggggctggcg ctcggggtgg tcaagcacct gactacgttc
     1861 accatgatcg gaatgctggt cggctacctc gtcggcgtgg cgttgacgcc gcggttcatt
     1921 tcgcagcagc gttacctcgc gatgtcggct gtgctcgggg cgctgttcgc cttcggcgcg
     1981 tgggcgacgc acggtcctct ttcggtggcg ttcgtggcgg cgctggggtt cgccaatgcg
     2041 atgatgtggc cggcgatctt cccgctcgcc atcaggggcc tgggcaagtg gaccgagttc
     2101 ggctcggcgc tcctcatcat gggcatcgcg ggcggcgcgt tgatcccgca actcttcgtg
     2161 cacctcgagg agatcatcga cttccagctc gcgttcctgc tggtgatggt gccgggatac
     2221 gcgtacattc tgttctacgg actgcacggt taccgcgccg gccagtacaa ccaggcacgc
     2281 gccatcgact ccgcgatcgc ggcgccttga cggcgatggc tgcatggcct gcgagaacct
     2341 cccgaacctg aaggagcatc cattggcaac cgccaccatc cgggacgtcg cgaagcaggc
     2401 caaggtgtcc ttgaagacgg tgtcgcgcgt gatcaatcgc gagggatcgg tacgcgaaga
     2461 gacgcgtgaa cgcgtgttgc gcgcggtcga ggcgctcgac tatcgccccg acctctccgc
     2521 gcgcagcctg cgcagcgcga aggcgtacgc gatcgggctg gtgtacgaca accccaaccc
     2581 ctattacatc atcgcggtcc agcagggcgc gctcgcggcg tgccgcgaga tgggcttcgg
     2641 gcttcagatc catccctgca actcgcgcgg cccgacgctc gccgaagagc tgcgcgatct
     2701 cgcgcgccgt tcgcggcttg ccggcctggt gctggcgccg ccgatgtccg agcacgtgga
     2761 gttgctgcgt gagctggcgg cgtacaatgt cccgctggtc cgcatcatct cggcggccga
     2821 ggatccgcgc gacgggtttc cgtgcgtgtt cgtggacgac cgcgacgcgg cctacgcgat
     2881 cacggcctac ctgatccagc tcggccatgt caggatcggc ttcctgtggg gcggcaagca
     2941 gcatcgttcc agcccggaac gctacaaggg ctacgaacag gcgctgcagg actacggcgt
     3001 gccgctgtct gacgaactgg tactggaagg cgactattcg ttcgacgacg gcttccgggg
     3061 cgctcgccga ttgctgggac tcgccgaacc gccgaccgcg atcttcggtt ccaacgacga
     3121 aatcgccgcc ggcgtgctgg cagccgcgcg tgcaggcgga ttgcatgttc cctacgacct
     3181 gtcggtggcc ggtttcgagg acagcccgtt ctcccgccat tcgtggccgt cgttgaccac
     3241 ttcgcgccag cgcaccgagg cgatcgccga acacgccacc cgactgctga tcgcgcagat
     3301 ccacggcgac gatgtcgaaa acgagggctt cagtccggag ctggtggtgc gcggttcgac
     3361 cgcgccgccg cggccgcgga cctgagcggc gcgtacgttt cgatgatgtc cgccacaccg
     3421 aatcgcgacc tccgacccga atcgacgttc ctgtaccggg aagccgccga ggcggccgat
     3481 gcggtcgcga ggcaactgga gcacaacgac gcttgcgtgc atcgactcgc gcaggcactg
     3541 cgcgattccc cgccgccgtt cgtcgtcacc tgcgcgcgcg gcagctccga ccacgcggcc
     3601 acctacgcca agtacgtgat cgaaacgcag accggctgcg ccaccgcctc ggcttcgccg
     3661 tcggtcgaat cggtctacgc ggtcccgctg aggctgcgcg gcgcgctgta catcgtgatc
     3721 tcgcaatcgg gcgcgagccc ggacctgctg cgcagcgccg aagccgcgcg cgaggccggc
     3781 gcgcgcgtgg tggcgctggt gaatgccgaa ggctcgccgc tggcgacgct cgccgacacc
     3841 gtgattccgt tgcgcgcggg catcgagcaa agcgtggcgg ccagcaagag cttcatcggc
     3901 tcgctggccg cgatcctgca cctggtggcg cactggcgcg ccgatgccgc gctgctgcgc
     3961 ggcctgcacg acctgccgga acaattgcgc cgggcattcg cgctggactg gtcgccgctg
     4021 gtggatgggc tcgccggcat gcatgacctg ttcgtgctag gccggggcta cggcctcggc
     4081 gcggcgcagg aagccgcgtt gaagctgaag gaaacgtgcg cgctgcatgc cgaggcattc
     4141 agcagcgcgg aggtccgcca tggccccatg gaactggtcg gccccgacct tccggtgctg
     4201 ctgttcgcgc aagccgatga cgcactggcg ggcgtgctcg cgaccgcgga ggattttcgc
     4261 agacgcggcg ccacggtgtg gacggcagcg cccggcgcat ccgggatggg cgccttgccg
     4321 atcgtccccg ctgtccagcc catgtgcgcg ccgatccttg cgatccagag cttctatcgc
     4381 gcggccaacg cgcttgcgct gcggcgcggg cgcgatccgg atcgtccttc gaacctcgcc
     4441 aaggtcaccg agaccgtgtg atgacgacgg cgctcgtcaa cgcacgggta ctcaccgcgc
     4501 agggatggcg cgacgatgtc gcggtggtcg tcgatggcgg ccgcatcctc gaactcgtgg
     4561 cgtccgatgg catcgcgttc ggcacggaag tccgggacct cggcggccgg atgctgctgc
     4621 cgggattcat cgattgccag gtcaatggcg gtggcggcgt gttgttcaac gaccagccga
     4681 cggtcgaggg catccgcgcg attggcgagg cgcatcgacg cttcggcacg acgggtttcc
     4741 tgccgacgct gatcagcgac acgtccgaaa agatgcaggc ggcgatcgcg gcggtgggtg
     4801 ccgcgatcgg ggaaggcgtc ccgggcgtgc tcggcatcca tctcgagggg cccttcctca
     4861 gccgcgaaaa gcgcggcgtc cacgcggagc agttcctgca tgcccctgac agcgtcgaat
     4921 tgcagacggc cgcttcactc cagcgcgggc tcaccttgct gacgctggca cccgaacgcg
     4981 tcgatcccga ggcgatccgt ggactcgccg aacgcggcgt cattatcgcc gcggggcatt
     5041 ccaatgccga ccatgacact gtgcgcgctg cgctggacgc tggcgtgcgc ggcttcacgc
     5101 acctcttcaa tgcgatgtcg ccgcttgcga gccgcgcgcc cggcatggtc ggcgcagcac
     5161 tcgaccacgc cgagagctgg tgcggtgtga tcgccgacgg ccaccacgtc cacccggcgg
     5221 cgatccgcat cgcgctgaag gcgaagccgc gagggaaaat cttcttcgtc accgatgcga
     5281 tgccacccgt cggtgcggac gatccatctt tcacgctggg cggcgagacg atcacggcac
     5341 gcgacggaat ctgccagacc gcgaacggca ccctcgcagg ttccgcgctc agcatgatcg
     5401 aggcgatccg caatgcggtc acgatgctgg gcgtaccgct cgaggaagcg gcgcggatgg
     5461 cgtcgaccta ccccgccaat ttcctcggcc tcggcgacac ccatggccgg atcgcgccgg
     5521 gctgtcgcgc caacttcacc gtgatcgatg ccgaactgcg cgtcacggaa acctggatcg
     5581 acggcgtacc gcaggcaaac tgaagtccaa gccggtttct gcgtcctcgc gggtttttcc
     5641 gaccgaccag cgaagcagcg gcttgggacg atcgcgtcgc ggccatgggc cgctcttaca
     5701 acatccaggc cacacgtaaa cacgcaggct cggcattgcc aagggcgcct gcaggagcgt
     5761 gcctgcgcga agcgcttgct ccttcttctg gacgcgacag gctgggtccc gccgattgaa
     5821 aaaaagaagc ccggcttgcg ccgggcttcc gaaccaccgg gccgtgctgg cgataccgcc
     5881 gccggcactt cccttgatta ctgctgctgt tgctgctggt cctggttcat gccctgacct
     5941 tccaccttga actcgacgcg acggttgcgc tgacggccag ccgcggtctt gttggtgtcg
     6001 atcgggtcgt tttcgccgaa gcccttgacc gcggtgatgc ggctcgaatc gatgccgtgc
     6061 gaggtcaggt actggtcgac gatgttggca cgacgctcgg acaggccctg gttgtactgg
     6121 tcggtaccga ccgaatcggt gtagccgtcg atttcgatct gcacctgcgg gtaccggttc
     6181 agggtgtcga ccgcctggtc caggatcgcg atcgaatcgg cttgcggcgg cttcaaggtc
     6241 ggaccgatgt cgctctcgcc cttcttcggg cgatcgaact tgaagttcac gccacgcagg
     6301 tcgatgacga cgttctgctg cggcggcggc ggcggcggag cagccggcgg cggcggcgga
     6361 gcagccggcg gcggcggcgg cggagccggg tggcccatgt tgaacaccag gcctacggtg
     6421 gccatcgtgt cgagccactg cttcggaccg taggtattcg gcagcccggg accggcaagc
     6481 ccggccagtt gggccgagtt gacgtcgcgg tagtagcgcg cggcgatttc gccacggacc
     6541 gcgatgcggt cactgacgtt gtagagcacg cccacgccga cggtcgcacc gggaccccag
     6601 ccggagttgc tgtgcggccc caggcacccg cattcgccgc ccgcctgggc cagctcttcg
     6661 gataccgctg catgacgcca gaatccgacg ccggccatca cgaacggacg ccactgcgaa
     6721 gtcggatcca tgaaatacca gcgaccggcg acgtcgaggc cgacggtttc ccactgcttg
     6781 cccgggcggg gggagtcgtc cttgaaatcc gcgttgtcac cggtggcttc gacgtccaac
     6841 gtgaagttgg gcgtgatcca gaaaccgaca ccgagtccac cgaccggatc ggtctgcacc
     6901 cgacgatcac tgtcgggaac caccacgccg atgcgcggaa cgatgtacca gttgctgtag
     6961 tcggggttgg tgaagctgcc cgttgattcc gtcgccatgc ccgtgtcctg cgcatgcgcg
     7021 actgacatca cgcccgcagt acccaaggcc agcgcgatga ccgcgaacaa gccattacgt
     7081 ttcatcggaa agtctcctcg ttgttgattc tttcgacgcc gaccgtccgg cggacgtcgt
     7141 gcttccagac cgcaaaacga ctgcgcgaaa aacggccctt cctgtcccgg ccctttccgc
     7201 tacggctgcg ccagtttagc aaaaaacaaa caattgcgcg gggctggcgt tcagccgttc
     7261 ataaaaagct caggcgcggg cttgcatggc gaattggtcc atgaacgccg gaaccggggt
     7321 gtccagcatg tcctcgggtt tttcgcaaag gccaatgatt tcagccgctt gcgcttcggg
     7381 aaggaatgcg gacagggcgc cccggaactt gcccaggaga accggaatgc cctcggcacg
     7441 gcggcgacgg tggccgatcg gatagtcgat ggaaaccttc gcggttttcg atccgtcctt
     7501 gaaaaaaacc tggactgaat tgccgatata acgcttgtcg ggatcgaaat aatcgcgggt
     7561 gaattgcgga ttttcacgca cgctcatccg gccgcgcaat gcatcgatgc gcggatcggc
     7621 cgcgacgaca tcgctgtaat cgtccgcaac caggcgaccg aaaatcagcg gcacggccac
     7681 catgtactgg atgcagtggt cgcgatctgc gtaattggcg agcggtccgg tcttgtcgat
     7741 gatgcgcgcg ccggcttcct gggtctcgat ctcgatccgc tcgatgtcgc cgatccggtc
     7801 cttcacttgc ggatgcagct tcatcgcgca ctcgaccgcg gtctgcgcgt ggaattcggc
     7861 ggggtaggag atcttgaaga ggatgttttc catcacgtac gaaccgaacg gccgctcgaa
     7921 ctcgaacggt tttccgccga acgcgacgtc gtagaaaccc caggtcttcg cgctcagcgc
     7981 cgagggatag ctcggctcgc ccgtggtcag cgcatccagc gcatggatca cggcgcggcg
     8041 gcacgcatcg cccgccgccc agctcttgcg gggtccggta ttgggcgcat ggcgataggt
     8101 ccgcaatgcg ccgttgtcga tccaactgtt cgacaccgcg gtgacgacct gcccgcgatc
     8161 gccgccgagc atcgcggtgg ccaccgcggt cgatgccagc cgcaccagga tcacgtggtc
     8221 catccccgct cggttgaaag aattcttcag cgcgtagcag ccctggattt cgtgcgcctt
     8281 gatcgcccag ccgagtacgt cgcggatcgt gagcggcttg tcgccattgc gcatgttgcg
     8341 gcgcgacagc cagtccgcga ccgccagcac cgcgccgagg ttgtcggacg gatggcccca
     8401 ctcggccgcg agccaggtgt cgttgaaatc cagccagcgg atctgggttc cgatacagaa
     8461 cgcagcctgc gcgggatcga gttcgagatc ggtgccgggt acccgggcgc cgccggccaa
     8521 cgtcgcaccg gacacgatcg gcgcgaggtg ccgcacgcac tgcgggaact tcatcgccag
     8581 catcgcgcac gccagcgcgt ccatcagcat gtagcgcgcg gtctcccacg cttcctgcga
     8641 ttcgacccgg tagtccagca cgtaatcggc gatgtcgagc aacggctggt cgggtgcggg
     8701 gcgggccgcg gaacggatgt cggcgtggct catggcgaat ttccgagcgg aaaacccgtc
     8761 attgtgccag agcgccctgc gcttgaccgc aaaccgaggg aagccgcaca atcgcccact
     8821 gccgaaaggg agtagcacgg gacccgggtc gatccgggtt cgcgtggcgg tcgtcaacac
     8881 gggcgcaaaa aaacgccccg gtcgccacgg cggatcgcga ccgcgcgcga gactttcgag
     8941 attccgcacc gcgtgtggga ttccgatcgt ccacgtaccg ggaagactgc atgccgccca
     9001 tcgaagccct gttgcaccat cccctcgaca gcgcgttctg gagcggcctc gcttcgatca
     9061 tcgtcatcga cctcgtcctg gccggcgaca atgccgtcgt gatcgcgctg gcggcgcggc
     9121 acctcccgaa gaaccaccag ttccgcgcga tcttctgggg cacggtgggc gcgatcgtgg
     9181 tcagggtcgc gatgacggcc ggcgtgatct ggttgctgcg gatcccggcg ctgatgctgg
     9241 tgggcggcct cgtgctgatc ccgatcgcgt tccggctgct caagcaggac gagcaggacg
     9301 acgacccgca cctgcggcac aaggtgaccg ggttctggag cgcgatgggc acgatcgtcg
     9361 tcgcggatgc gctgatgggc ctcgacaacg tgctggcgat cgcgggtgca tcgaacggcc
     9421 atctcggact cgtcgcgctg ggcttgctga tcagcgttcc gctggtggtg tggggttcga
     9481 ccctgctgtt gaagctgatc gagcgattcc cggtgatcat ctacatcgg
//
LOCUS       DASSNH010000211         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_22757, whole genome shotgun sequence.
ACCESSION   DASSNH010000211 DASSNH010000000
VERSION     DASSNH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22757"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFK00_07690"
     CDS             <1..853
                     /locus_tag="VFK00_07690"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592867.1"
                     /translation="QRAVPELAVVADSFHTKPLMRIMQSADRFQVLGLNRHEARLFEG
                     NRDRIAEIDLAPDVPRSLEDALEREFGRDRDIRTYGPIRDGTMGRHGKSDVKQDAIRA
                     DTEQFFRIIDRGVFEHHSRPTGLPLLLAALPEHHHMFREVSENPHLLEETIDVFPGDV
                     SLDELRERAWDKVLPHYLARQEAFIDRFHAATPRGQATADLADAARAVAESRIATLLL
                     DAGRQVPGRMDGDSGEIILGKLDDPHVDDLLDDLGERVLATGGEVVVVPSERMPTETG
                     LAAIFRY"
     gene            1001..1708
                     /gene="tsaB"
                     /locus_tag="VFK00_07695"
     CDS             1001..1708
                     /gene="tsaB"
                     /locus_tag="VFK00_07695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7592868.1"
                     /translation="MNILAIETATEACSVALLHGDALIGRSEIAPRRHAELVLPMAES
                     LLAEAGIVRRQLDAIAVGRGPGAFTGVRLAISVAQGLALALDVPVLPVSSLAALAMQA
                     PANGAAVLAAIDARRGEIYAGAFRLDEAGLAEPLDEERVLPAADLALPGADAWNVIGS
                     GWGAYGDSIRERLPAPPHWAEGERYPQARDVVRLAAPRFAAGEGVPPEQALPVYLRDK
                     VALTLREQRHARTGDAL"
     gene            complement(1789..2172)
                     /locus_tag="VFK00_07700"
     CDS             complement(1789..2172)
                     /locus_tag="VFK00_07700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7592869.1"
                     /translation="MPLHVRWRDLDAFNHVNNSSYLTFLEEARLQWLRTVPGEWFTEH
                     AMPVMAAVELHYRKPITWPSEVDVLLSCERLGNTSITIGNRIVDRNDATCVYADGNIV
                     MVWVDPASGAAVPLPEAIRAACAAP"
     gene            complement(2471..>5096)
                     /gene="uvrA"
                     /locus_tag="VFK00_07705"
     CDS             complement(2471..>5096)
                     /gene="uvrA"
                     /locus_tag="VFK00_07705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7592870.1"
                     /translation="AISIEQKSTSHNPRSTVGTITEIHDYLRLLYARVGTPRCPRHGT
                     SLEAQTVSQMVDATLALPADKRFMLLAPVIRERKGEHVQVFEQLRAQGFVRARVDGNV
                     YDLDAVPALGLRIKHTIEVVIDRFRPRADLKQRLAESFETALRLGEGLAILVDLDDEK
                     APEQLFSSRYSCPQCDYSLPELEPRLFSFNSPVGACPACDGLGVTQFFDPARVVAHPE
                     RSLANGAIRTWDRRNPYYWQMVQSLAKHYRFDPETPWNRLPAATREAVLNGSGNETIA
                     FRYTPGHSGGPVTRRHRFEGIIPNLERRYRETDSTAVREELARFISERPCPECHGERL
                     NLSARNVFVGEKNLPSLSALAISDARGFVDGLQLAGWRGEIAERIVKEIRERLRFLID
                     VGLDYLTLDRKADSLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLGT
                     LTRLRDLGNTVIVVEHDEDAIRTADRIVDIGPGAGVHGGEVIAQGSLADILAAPRSLT
                     GQYLSGKRSIEVPRLRRPPDPRRVLKLKGAHGHNLQHVDLEIPAGLFTCITGVSGSGK
                     STLVNDTLFRIAAVELNGASAQPSPYASIEKLDLFDKVLDIDQSPIGRTPRSNPATYT
                     GVFTPLRELFAQVPEARARGYKPGRFSFNVKGGRCEACEGDGLIKVEMHFLPDMYVSC
                     DVCHGKRYNRETLEILYKGYTIADVLDMTVEQALEVFAPVPQIARKLETLMDVGLGYI
                     KLGQSATTLSGGEAQRVKLSKELSRRDTGNTLYILDEPTTGLHFLDIEQLLRVLHRLV
                     DGGSTVIVIEHNLDVIKTADWIVDLGPEGGAGGGRIIAAGTPEDIATTPGSYTGQFLA
                     PVLNAASTSKPSRRKKRA"
BASE COUNT          778 a         1698 c         1757 g          863 t
ORIGIN      
        1 gcagcgagcc gtgccggaac ttgcggtggt cgccgacagc ttccatacca agccgctgat
       61 gcggatcatg caatccgcgg accgcttcca ggtgctcggc ctgaaccgcc acgaggcgcg
      121 cctgttcgaa ggcaatcgcg atcggatcgc cgagatcgat ctcgcgccgg acgtgccgcg
      181 ttcgctcgag gatgcgctgg aacgcgaatt cgggcgcgat cgcgatatcc gcacctacgg
      241 ccctatccgc gacggcacga tgggccgcca tggcaaaagc gacgtgaaac aggacgcgat
      301 tcgcgccgat accgaacagt tcttccggat catcgaccgc ggcgtgttcg aacaccactc
      361 gcgtccgacc gggctgcctc tgctgctggc cgcactgccc gaacaccacc acatgttccg
      421 ggaagtcagc gagaatccgc acctgctcga ggaaaccatc gacgtgtttc cgggcgacgt
      481 cagtctcgac gaactgcgcg agcgcgcctg ggacaaggtg ctgccgcact acctggcacg
      541 gcaggaagcc ttcatcgacc gcttccacgc ggcgacgccg cgcgggcagg ccaccgccga
      601 tctcgcggat gccgcgcgtg cggtggcgga gagccgcatc gcgaccctgt tgctggacgc
      661 cggacgccag gttcccgggc ggatggatgg agacagcggc gaaatcatcc tcggcaagct
      721 cgacgatccg cacgtggacg acctgctcga cgacctcggc gagcgcgtgc tcgcgacggg
      781 cggagaggtg gtcgtggtgc cgagcgagcg catgcccacc gaaaccggcc tcgcggccat
      841 ctttcgctac tgactcgggc gtggaccctt tccgtcaggc ccgtgaggct gacaccgtcc
      901 gacgccccct gcagggcggc aaccgccagg cgggccgggc tgtggtcggc attgctgcta
      961 cgcttcgtcg attctcggtt cgggatcgcg ttcgcgcagc atgaacatcc tcgccatcga
     1021 aacagccacg gaagcctgct cggtcgcgct cctgcacggc gatgcattga tcggccgcag
     1081 cgaaatcgca ccgcggcgtc acgcggagct ggtgctgccc atggcggaat cattgctggc
     1141 cgaagccggg atcgtgcgcc gccagctcga cgcaatcgcg gtcgggcgcg ggccgggtgc
     1201 gttcaccggg gtccggctgg cgatctcggt ggcgcagggc ctggctctgg cgctggacgt
     1261 gccggtactg ccggtttcgt ccctggcggc gcttgcgatg caggcaccgg cgaacggcgc
     1321 cgccgtactg gccgcgatcg acgcgcgtcg cggggaaatc tacgccgggg cattccgtct
     1381 ggacgaagcg ggattggccg agccgctgga cgaagagcgc gtgctgccgg ctgccgacct
     1441 cgcgttgcca ggggccgatg cctggaacgt gatcggcagc ggctggggcg catatggcga
     1501 ctccatccgc gagcggttgc ccgcgccgcc gcactgggcc gaaggcgaac ggtatcctca
     1561 ggcgcgcgat gtcgtgcgcc tggccgcgcc acgttttgcc gccggcgaag gcgtaccgcc
     1621 cgaacaggcg ctgccggtat acctgcgcga caaggtggcg ttgaccctcc gggagcaacg
     1681 acacgcccga accggcgacg cgttgtagga ccgcgcctgc gcgaagcact tgctccttct
     1741 cttgcgcccg atcagggttg cacgatcgcg agaccgggcg cgccgctcct acggggcggc
     1801 gcaagccgcg cggatcgcct cgggcagcgg caccgccgcg ccgctcgcag gatccaccca
     1861 caccatcacg atattgccgt cggcgtagac gcaggttgcg tcattccgat ccacgatgcg
     1921 gttgccgatc gtgatggagg tgttgccgag ccgttcgcag ctcagcagca cgtcgacttc
     1981 cgaaggccag gtgatcggct tgcggtagtg caattcgacc gctgccatca cgggcatcgc
     2041 gtgttcggtg aaccattcgc cgggcaccgt gcgcagccat tgcaggcgcg cctcctcgag
     2101 gaaggtgagg tacgacgagt tgttgacgtg gttgaacgcg tcgaggtcgc gccagcgcac
     2161 gtgcagcggg atggtcgcga gggtgcggga gaccgccggc ggagcgtcgc cggactgcga
     2221 cggatcttgt tgcaccgcac ccggacccgg ggcttgcgcc cttgctttgc cgcgctttcg
     2281 gggtgcacgc ctggccgggc ggttttgctt gtcgttgttc atgtccatga cttttcggtt
     2341 ggggccggtg tgtcttcgtc attccgcggc catcgtcggc tccatcgcct ctgctgccgg
     2401 caaacagcgc ggccagcccc ggaatgacca cggcgaaggc gcaggcttgc tgccgactcc
     2461 gactttctcc tcacgcgcgc ttcttcctgc gcgagggctt cgacgtcgaa gcggcattca
     2521 ggaccggcgc gaggaattgg ccggtgtacg agcccggcgt ggttgcgatg tcttccggcg
     2581 tgccggcagc gatgatgcgt ccgccgccgg cgccaccttc ggggccgagg tcgacaatcc
     2641 agtcggcggt cttgatcacg tcgaggttgt gctcgatcac gatcacggta ctgccgccat
     2701 cgaccagccg gtgcagcacg cgcagcagtt gctcgatgtc gaggaaatgg aggccggtgg
     2761 tcggctcgtc gaggatgtac agggtgttgc cggtgtcgcg gcgcgagagt tccttcgaca
     2821 gcttcacgcg ctgcgcttcg ccgcccgaca gcgtggtcgc gctctggccg agcttgatgt
     2881 agcccaggcc cacgtccatc agcgtttcca gcttgcgcgc gatctggggc accggcgcga
     2941 atacttccag cgcctgctcc accgtcatgt cgagcacgtc ggcgatcgtg tagcccttgt
     3001 agaggatctc cagcgtctcg cggttgtagc gcttgccgtg gcagacgtcg caggacacgt
     3061 acatgtccgg caggaagtgc atttccacct tgatcagccc gtcgccttcg caggcctcgc
     3121 accggccgcc cttgacgttg aagctgaaac ggcccggctt gtagcctcgc gcgcgcgctt
     3181 ccggcacctg cgcgaacagt tcgcgcaggg gggtgaacac gccggtgtac gtggcgggat
     3241 tcgaacgcgg cgtgcgcccg atcggcgact ggtcgatgtc cagtaccttg tcgaacaggt
     3301 ccagtttttc gatggatgcg tacggcgacg gctgcgccga tgctccattc aattccaccg
     3361 ccgcaatccg gaacaaggtg tcgttcacga gcgtggattt gcccgagccc gacacgccgg
     3421 tgatgcaggt gaacaggcca gccgggattt cgaggtcgac gtgttggagg ttgtggccat
     3481 gcgcgccctt cagtttcagc acgcgcctgg gatccggtgg gcggcgcagc cgcggtacct
     3541 cgatcgaacg tttgcccgac aggtactgtc ccgtcaacga tcggggcgcg gcaaggatgt
     3601 ccgcgagcga accctgcgcg atcacctcgc cgccgtgcac gccggcgccg ggcccgatgt
     3661 cgacgatccg gtcggcggtg cggatcgcgt cctcgtcgtg ttcgaccact atcacggtgt
     3721 tgccgaggtc gcgcaggcgg gtcagcgtgc cgagcaggcg ctcgttgtcg cgctggtgca
     3781 ggccgatgga cggttcgtcc agcacgtaca tcacgccgac caggccggcg ccgatctgcg
     3841 aggccaggcg gatgcgctgc gcttcgccgc ccgagagcga gtcggccttg cggtcgagcg
     3901 tgaggtaatc gaggcccacg tcgatcagga atcgcaggcg ctcgcggatc tccttgacga
     3961 tgcgctcggc gatctcgccg cgccagcccg cgagctgcag gccgtcgacg aagccgcgcg
     4021 cgtcgcttat cgccagcgcc gacagcgacg gcaggttctt ctcgcccacg aacacgttgc
     4081 gcgcggaaag attcagccgc tcaccgtggc attccgggca gggccgttcg ctgatgaagc
     4141 gcgcgagttc ctcgcgcacc gcggtggaat cggtctcgcg gtaacggcgt tcgaggtttg
     4201 ggatgatgcc ctcgaaacgg tggcggcgcg tgacgggacc acccgaatgg cccggcgtgt
     4261 agcggaaggc gatcgtctcg ttgcccgagc cgttcagcac cgcctcgcgc gtggctgcgg
     4321 gcagcctgtt ccagggcgtc tccggatcga agcggtagtg cttcgccagc gactgcacca
     4381 tctgccagta ataagggttg cgccggtccc aggtccggat cgcgccgttg gcgagcgaac
     4441 gctccggatg cgcgaccacc cgggcgggat cgaagaactg ggtgacgccg aggccgtcgc
     4501 acgcggggca cgcgcccaca ggcgagttga acgagaacag ccgcggctcc agctccggca
     4561 gcgagtagtc gcactgcgga cacgaatagc gcgacgagaa cagttgttcc ggcgccttct
     4621 cgtcgtcgag gtcgaccagg atcgccagcc cctcgcccag ccgcagcgcg gtttcgaacg
     4681 attcggcgag gcgctgcttc aggtccgcgc gcggacggaa gcggtcgatc accacctcga
     4741 tcgtgtgctt gatgcgcagg cccagcgccg gcacggcgtc caggtcgtac acgttgccat
     4801 cgacgcgcgc gcgcacgaag ccctgcgcgc gcaactgctc gaacacctgc acgtgttcgc
     4861 ccttgcgttc gcggatcacc ggcgccagca gcatgaagcg cttgtcggcc ggcagcgcca
     4921 gcgtggcatc caccatctgg ctgaccgtct gcgcttccaa tgatgtgccg tggcgcgggc
     4981 agcgcggcgt gcccacgcgc gcgtacagca ggcgcaggta gtcgtggatc tcggtgatgg
     5041 tgccgaccgt cgatcgcggg ttgtgcgacg tcgacttctg ctcgatcgaa atcgcc
//
LOCUS       DASSNH010000212         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1053241, whole genome shotgun sequence.
ACCESSION   DASSNH010000212 DASSNH010000000
VERSION     DASSNH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053241"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1171
                     /gene="tsaD"
                     /locus_tag="VFK00_07710"
     CDS             146..1171
                     /gene="tsaD"
                     /locus_tag="VFK00_07710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7592871.1"
                     /translation="MLGIESSCDETGVAVYEPGRGLLAHALYSQISLHAEYGGVVPEL
                     ASRDHVRKLLPLLRQTLADADLGVRDLGAVAYTAGPGLIGALLVGASVARSLAWALEI
                     PAIGVHHMEGHLLAPLLEEPAPGTEPLAPPFVALLVSGGHSLVVGVEQIGSYTVLGDT
                     LDDAAGEAFDKTAKLMGLPYPGGPALAKIAETGKPGAFAFSRPMTDRPGLDMSFSGLK
                     TQVLLAWRDSDRADDTRAGIARGFEEAIVETLAIKCRRALAQTGYKRLVVSGGVGANR
                     RLRAQLAEAGQREGFQTYFPRLQFCTDNGAMIALAGAIRLAAGQHTGPEIQVRPRWDL
                     QTLPAVA"
     gene            1176..1535
                     /gene="folB"
                     /locus_tag="VFK00_07715"
     CDS             1176..1535
                     /gene="folB"
                     /locus_tag="VFK00_07715"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7592872.1"
                     /translation="MDTVFIEGLTVETVIGIYDWERRVRQPVVLDVEMAFDNTRPAAS
                     DRIDDTLDYKAVSKRLIGFIEDSSFQLVETLAERCAAIIRDEFGVAWVRLRLAKPGAV
                     RGARAVGVVVERGSRNA"
     gene            1657..>2013
                     /gene="folK"
                     /locus_tag="VFK00_07720"
     CDS             1657..>2013
                     /gene="folK"
                     /locus_tag="VFK00_07720"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7592873.1"
                     /translation="MPRVYLSLGSNLDAERHLREAVGALRARFDDVVVSPVYRSEAVG
                     FEGAPFLNCAAAFDADLAHDPLRAWLRELEDASGRDRSLPRYADRTLDLDIALWCEGG
                     ACNSDLSREEFERAHVL"
BASE COUNT          305 a          666 c          699 g          343 t
ORIGIN      
        1 tgaagcggcg cagggcgatt tcgaacggct cgttctcgcg gactttgacg ctgggcatgg
       61 acgctccact tggtaaactg gcccgcttgg ccgcgggtga gccgcgaatt atagcgtgat
      121 ttccgtgatc gattcaaagt ccccggtgct gggcatcgag agctcctgcg acgaaaccgg
      181 cgtggcagtt tacgaacccg ggcgcgggct gctggcgcac gccctgtatt cgcagatttc
      241 cctgcacgcc gaatatggcg gcgtggtccc ggagctggcc agccgcgacc acgtcaggaa
      301 gctgcttccc cttttgcgcc agacacttgc ggacgcggac ctcggcgtcc gggatctcgg
      361 cgcggtggcc tataccgccg ggcccggcct gatcggcgcg ctgctggtcg gcgcctccgt
      421 ggcccgttcc ctggcctggg cgctggagat ccccgcgatc ggggtccacc acatggaggg
      481 gcacctgctg gcgccgctgc tggaggaacc cgcgccgggc accgaaccgc tggcgccccc
      541 gttcgtggcg ctgttggtgt cgggggggca ttcgctggtg gtcggggtcg agcagatcgg
      601 cagctacacg gtcctcggcg atacgctgga cgacgccgcg ggcgaagcct tcgacaagac
      661 cgccaagttg atgggcctgc cgtaccctgg tggtccggcc ttggcgaaga tcgccgaaac
      721 cgggaagccg ggggcattcg cattttcgcg cccgatgacg gaccggcccg gcctcgacat
      781 gagtttttcc ggcctgaaga cccaggtcct gctggcgtgg cgggacagcg accgggccga
      841 cgacacccgg gcgggcatcg cgcgcggttt cgaggaagcg atcgtggaaa cgctggcaat
      901 caagtgccgg cgcgcgctcg cgcaaacggg gtacaagcgg ctggtggttt ccggcggtgt
      961 cggcgccaac cggcgcctgc gtgcgcaact cgccgaagcc gggcagcgcg aaggcttcca
     1021 gacgtatttc ccgcgccttc agttctgcac cgacaacggc gcgatgatcg cgctggcggg
     1081 tgcgatccgg ctcgccgccg gccagcacac cggccccgaa atccaggtgc ggccgagatg
     1141 ggatctgcag accttgcctg cggtagcctg agcgcatgga taccgtcttc atcgaaggcc
     1201 tgaccgtcga aaccgtcatc ggcatctacg actgggaacg cagggtgcgc cagccggtgg
     1261 tgctcgatgt cgaaatggcc ttcgacaaca cacgtccggc cgccagtgac cgcatcgacg
     1321 acacattgga ttacaaggcc gtatccaaac gcctgatcgg tttcatcgag gactcgagtt
     1381 tccagttggt ggagaccctc gccgagcgct gcgccgccat catccgtgac gagttcggtg
     1441 tcgcgtgggt gcgactgagg ctcgccaagc ccggcgccgt ccgcggtgcg cgggcagtgg
     1501 gcgtcgtggt cgaacgcggc tcgaggaacg cgtagctgct gtgcctggta gaccgacatt
     1561 gcccgccggg cattcggcgg ctgacggtgt ccgcccaaca ccccggtggc ccgccatccg
     1621 ctttgctcac gacattcctt cggaagtccc gaacccttgc cacgcgttta cctgagcctc
     1681 ggttccaacc tcgacgccga gcgccacctg cgcgaagcgg tcggcgcgtt gcgcgcgcgc
     1741 ttcgacgatg tcgtggtgtc gcccgtgtac cgcagcgaag cggtgggatt cgagggggcg
     1801 ccgttcctca attgcgcggc ggccttcgat gccgatctcg cgcacgatcc gttgcgcgca
     1861 tggctgcgcg agctggagga tgccagcggc cgcgatcgca gcctgccgcg ctacgcggac
     1921 cgcaccctcg acctcgacat cgcgctgtgg tgcgaaggcg gtgcgtgcaa cagcgacctg
     1981 tcgcgcgagg aattcgaacg ggcgcacgtg ctg
//
LOCUS       DASSNH010000213         6357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_268300, whole genome shotgun sequence.
ACCESSION   DASSNH010000213 DASSNH010000000
VERSION     DASSNH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268300"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1197
                     /gene="gshB"
                     /locus_tag="VFK00_07725"
     CDS             226..1197
                     /gene="gshB"
                     /locus_tag="VFK00_07725"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET7592874.1"
                     /translation="MNSTVAVLMDPIGTIHPEKDTTLALLLEAARRGHALRYMGQGDL
                     AVRDGVAWARLSPLEVRNDRSDWFTLGEPRWQPLAEAADIVLARKDPPFDAQFLYDTL
                     VLDLAARDGARVVNEPQALRDFNEKLAAQWFPQCCAPALVARDRGELRRFAREHGEVV
                     LKPLDGMGGRGIFRSRGDDPNLNSILETLAGDDRRFVMAQKFIPEITAGDKRILVVDG
                     EAVPYALARIPQGTDFRGNLAVGGRGVGQPLSERDRWIAAEVGPELTRRGIVLAGLDV
                     IGDYLTEVNVTSPTCVCELDAQFGLNIAGSVFDAIEAGTGFPSELSA"
     gene            1194..2102
                     /locus_tag="VFK00_07730"
     CDS             1194..2102
                     /locus_tag="VFK00_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7592875.1"
                     /translation="MNAATASMAHGTGHADRMGVALLFSLIVHAIVILGLGFQFAKPA
                     PSLPTLDVTLINTSNAEAPTKADFLAQADNAGGGNSDKAHRPGAPFSGPLPVANAGIA
                     PVAVMPGAPARQKATGPHILTTTGKASRSTPSERDTRDQPQAEQRTAAVPIPQRLEMA
                     RLAQEVRREQEAYARRPRRKYLSANTRGTTFAAYQVAWVDRIERIGNLNYPDAARRRH
                     LHGNVMLTVVLGMDGHVREVTVNQSSGYRVLDEAAVRIVHLAAPFPRIPQDSDANGNR
                     YNELVITRTWQFLPGNHLETQAVRSR"
     gene            2253..2777
                     /locus_tag="VFK00_07735"
     CDS             2253..2777
                     /locus_tag="VFK00_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET7592876.1"
                     /translation="MPGMGDPNFARGITFICQHNQDGAMGLVVNRLSDYRLGDVLQQM
                     NMPCGRKSVASAPVLVGGPVQPERGFVLHAAGQREWESSYRINDALAVTTSRDILAAM
                     ADGDGPGRVLVALGYAGWGAGQLEQELRENAWLTVQADDHILFDTALDERWGAAVALV
                     GVNPANLTSYFGRA"
     gene            2774..3199
                     /gene="ruvX"
                     /locus_tag="VFK00_07740"
     CDS             2774..3199
                     /gene="ruvX"
                     /locus_tag="VFK00_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7592877.1"
                     /translation="MTGCVLGFDYGARRIGVASGNRVVATAQPLPALVASAGKPDWTR
                     IDALLAEWKPEALVVGLPLALDGGEQAITRAARAFAAALGQHSGLPVHLVDERHTSQE
                     AARRFAAQRASGAARRRDAANIDSLAAAVILESWLQAGT"
     gene            3298..4209
                     /locus_tag="VFK00_07745"
     CDS             3298..4209
                     /locus_tag="VFK00_07745"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7592878.1"
                     /translation="MRHLLDLEDLDRTAINRLLDRADRFRSEGAPRDLLVGRTVLTLF
                     FEPSTRTRTSFVLAAKRLGADNVNFDLSRSSTIKGESLLDTLHTLEAMRLDALVVRHN
                     ENGMPAYLAEHARSGVAVVNAGDGTHAHPTQGLLDVLTIRQHRNDFANLRVTICGDIR
                     HSRVARSDVRALATLGTRDIRLCAPSAMLPESLQEFPGCTTSDDFDAAIDGADVVVML
                     RIQKERMLDAQFPDTAGYHAHYGLDTRRLALAAKDCQVLHPGPINRGIEIASEIADGT
                     QSRILDQVANGVWVRMGVLAELLAVPA"
     gene            4243..5922
                     /locus_tag="VFK00_07750"
     CDS             4243..5922
                     /locus_tag="VFK00_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592879.1"
                     /translation="MRPVLRRTAFAAACLAALVLAGCGKHVASDAPITYAPADTPYLF
                     ANFKGQPADVAEAWSGVGNSGLQGRIQQLDQIGKLTRQKDPAVAKVVDAVQAELANVQ
                     TNKELAATLGLSQSPLYAMYGVGDVPVMRVELDSPDTFKAFWARVEKRAGITVPTATL
                     DKQSYWVIGGAGAKVRILVAIEGKQLVATVAPANAGPDMLKQLLGLAKPSSNASDRLS
                     DLNGKQGYGDYGSGYVDLARLFSNLFDGKDAITQEFAKDLGGSLANPSCASEFASLAQ
                     QVPMVSAGVKSATAQKVSLSIDARLSPSLLGALTALKHQVPGMGEASDTSMFDMVLAL
                     PLTQWQAFLQGRAKAAAGKTYRCPALQSLNRFAQTAAHPPVQMPPEAASLMGFRIVLD
                     QWEAGPQIAGRLLVASSDPAALAQKIQQTLPQFALKPIPTNGQPVAFDLPPRLRALLG
                     GGSQGWIAANDKALAIGVGDDEQTKLAGTLTASTGNGDRLFRMHFDGKMYGVLGSWMD
                     RFSAMAPAASQAQLQQQVATFQQMGKMVASVDADGRLDGDGLHFDIDVTHR"
     gene            complement(6039..>6357)
                     /locus_tag="VFK00_07755"
     CDS             complement(6039..>6357)
                     /locus_tag="VFK00_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HET7592880.1"
                     /translation="RGRRVAMEVMLGTPLVQDYIRKGEIHKIKDVMKQSTNLGMCTFD
                     QSLVELYHAGNISYEDALRYADSSNEVRLAIKLAQGGDAHSLSQGLGDVELTETIERG
                     HRL"
BASE COUNT         1075 a         2199 c         2114 g          969 t
ORIGIN      
        1 cgtcacgtga tccgcacgcc gtcgctcgtg atccgttccc cggcggatgc catacccggc
       61 aaaaccggcc gcgtatgcca agatacacca agagccgccc gttcgaatgt ggacggattt
      121 gcgcttcggc cgaccggccc gacctccacc ccattgactt gccgctgatc caaccccggc
      181 acctcccatg acttcagtcg agttgctgat ccgccccacc agcccatgaa ttccaccgtc
      241 gcggtgctga tggaccccat cggcaccatc catccggaaa aggacaccac cctcgcgctg
      301 ctgctggaag cggcgcgccg cggccatgcg ctccgttaca tgggccaggg tgacctcgcg
      361 gtgcgcgacg gcgtggcgtg ggcgcggttg tcgccgctgg aggtgcgcaa cgaccggagc
      421 gactggttca cgctcggcga gccacgctgg cagccgctcg ccgaggccgc ggacatcgtg
      481 ctcgcgcgca aggatccgcc gttcgatgca cagttcctct acgacaccct ggtgctggac
      541 ctcgcggcgc gcgacggggc gcgcgtcgtc aacgaaccgc aggcgctgcg cgacttcaac
      601 gagaaactcg cggcgcagtg gttcccgcaa tgctgcgcgc cggcgctggt ggcgcgcgac
      661 cgcggcgaac tccgccgctt cgcgcgcgaa cacggcgagg tcgtgctgaa gccgctggat
      721 ggcatgggcg ggcgcggcat cttccgttcg cgcggcgacg atcccaacct caactcgatc
      781 ctggaaacgc tggccggcga cgatcgccgg ttcgtgatgg cgcagaaatt catccccgag
      841 atcaccgccg gcgacaagcg gatcctggtc gtggacggcg aggccgtgcc gtacgcgctg
      901 gcacgcatcc cgcaaggcac cgacttccgc ggcaacctcg cggtgggtgg acgcggcgtc
      961 ggccagccgc tgtccgagcg cgaccgctgg atcgcggccg aggttgggcc ggaactgaca
     1021 cggcgcggca tcgtgctggc cggactggac gtgatcggcg actacctcac cgaagtcaac
     1081 gtcacctcgc ccacgtgcgt ttgcgagctg gacgcgcagt tcgggttgaa cattgcgggc
     1141 tcggtgtttg atgcgatcga agcggggacc ggcttcccta gcgagttgtc agcgtgaacg
     1201 ccgcgacggc ctccatggcg cacggcaccg gccacgccga ccgcatgggc gtcgcgctgc
     1261 tgttctcgct gatcgtgcac gcgatcgtga tactagggct gggattccag ttcgcgaagc
     1321 cggcgccgag cctgccgacg ctcgacgtca ccctcatcaa tacctcgaac gccgaggcac
     1381 cgaccaaggc ggatttcctg gcgcaggccg acaacgccgg cggcggcaac agcgacaagg
     1441 cacacaggcc cggggcgccc ttctcggggc cgctgccggt cgcgaacgcc ggcatcgcac
     1501 cggttgcggt aatgccgggg gcgccggccc ggcagaaggc gaccggcccg cacatcctca
     1561 cgaccaccgg caaggccagc cggagcacac cgagcgaacg cgatacccgg gaccagccgc
     1621 aggccgagca gcggacggcg gcggttccga ttccgcagcg cctcgaaatg gcacgactcg
     1681 cacaggaagt gcggcgcgag caggaagcct acgcgcgccg tccgcgccgg aaatatctct
     1741 ccgcgaacac ccgcggcacg acgttcgccg cctaccaggt cgcctgggtg gacaggatcg
     1801 agcgtatcgg caacctcaac tatccagacg ccgcacgccg caggcacctg cacggcaacg
     1861 tgatgctcac ggtggtgctc ggcatggacg ggcacgtgcg cgaagtcacg gtgaaccaga
     1921 gctcgggcta tcgcgtcctc gacgaagccg ccgtgcggat cgtgcacctc gcggcgccct
     1981 ttccccggat cccgcaggac agcgacgcca acggcaaccg ctacaacgaa cttgtgatca
     2041 cccgcacatg gcaattcctg cctggtaatc atcttgaaac acaggccgtc cgttcacgct
     2101 gatcggccgc ctggccgcgt tacaatcggg ccatggccac caagtcacca cgcacttccc
     2161 gcagcagccg ttcccggccg caaaaggcgg acgacgcggg caccatccgc accgccgaac
     2221 cggcatcgct gaccgggcat ttcctgatcg ccatgccggg catgggcgat ccaaacttcg
     2281 cacgcggcat caccttcatc tgccagcaca accaggacgg cgcgatggga ctggtcgtca
     2341 accggttgtc cgattaccgg ctcggtgacg tgctgcagca gatgaacatg ccctgcgggc
     2401 ggaagtccgt cgcctcggcc ccggtgctgg tgggcggtcc cgtgcaaccg gagcgcggct
     2461 tcgtcctgca cgcggcgggc cagcgcgagt gggaatcgag ctaccgcatc aacgacgcac
     2521 tcgccgtcac gacctcgcgg gacatcctcg ccgcgatggc ggatggcgat ggtccggggc
     2581 gggtgctggt ggcgctcggc tacgcaggct ggggcgcggg ccagctcgag caggaactgc
     2641 gcgaaaacgc ctggctgacg gtgcaggccg acgaccacat cctgttcgac accgcgctcg
     2701 acgagcgttg gggcgccgcc gtggcgctgg tcggggtcaa ccccgcgaac ctcacgagct
     2761 acttcggtcg ggcgtgaccg gatgcgtgct ggggttcgat tacggcgccc gccggatcgg
     2821 agtcgccagc ggcaaccgcg tcgtcgccac cgcgcaaccg ctgcccgccc tcgtcgccag
     2881 cgccggcaag ccggactgga cgcgaatcga cgcgctgctg gcggagtgga agccggaagc
     2941 attggtcgtc ggcctgccgc tggcgctgga tggcggcgag caggcaatca cccgcgccgc
     3001 gcgggcattc gccgcggcac tgggccaaca cagcggactg ccggtgcacc tggtcgacga
     3061 gcgccacact tcgcaggaag ctgcacgcag gttcgccgcc cagcgcgcct ccggcgccgc
     3121 gcggcggcgc gatgccgcga acatcgattc gctcgccgcc gccgttatcc ttgaaagctg
     3181 gctgcaggcc gggacataac cgaaaccggg gtcagagtgc atttttcgct gctcgtggac
     3241 gtgcggtcca tgaaacggcg tcgaaaagta cactccgacc ccatatcatg cgcggcgatg
     3301 cgacacctcc tggatctcga agacctcgac cgcactgcga tcaaccggct gctcgatcgc
     3361 gcggaccgct tccgcagcga gggcgcgccg cgcgacctgc tggtcgggcg caccgtactg
     3421 acgctgttct tcgagccatc cacgcgaacc cgcacctcct tcgtgctggc ggccaagcgg
     3481 ctgggcgcgg acaacgtcaa cttcgatctt tcgcgctcgt ccacgatcaa gggcgaaagc
     3541 ctgctcgaca cgctgcacac gctggaggcg atgcgcctcg acgcgctggt ggtgcggcac
     3601 aacgagaacg gcatgcccgc gtacctcgcc gaacacgcga ggagcggcgt cgcggtggtg
     3661 aatgccggcg acggcaccca cgcgcatccg acccagggcc ttctcgacgt gttgacgatc
     3721 cgccagcacc gcaatgattt cgcgaacctg cgcgtgacga tctgcggcga catccgccat
     3781 tcgcgggtgg cgcgttcgga cgtgcgcgcg ctggcaaccc ttggaacacg cgacatccgc
     3841 ctgtgcgcgc cgagcgcgat gcttcccgag tcgctgcagg aattccccgg ctgcacgacc
     3901 agcgacgatt tcgatgccgc gatcgacggc gcggacgtcg tggtcatgtt gcgcatccag
     3961 aaggaacgga tgctggacgc ccagttcccg gataccgctg gctaccacgc gcactacggg
     4021 ctcgacaccc gcaggctcgc gctggccgcg aaggattgcc aggtgctgca cccgggaccc
     4081 atcaaccgcg ggatagagat cgcgtcggag attgctgacg ggacgcaatc gcggatcctg
     4141 gatcaggtcg cgaacggtgt ctgggtgcgg atgggggtgc tggcggagtt gctggcggtc
     4201 ccggcataat cgggacctac aaggggaaac gggagaacgc ccatgcgtcc agttctgcgc
     4261 cggacggcct tcgcggccgc ctgccttgcg gccttggtcc tcgcgggttg cggcaagcac
     4321 gtcgcctccg acgcgcccat cacctacgcg cccgccgaca cgccctacct gttcgccaat
     4381 ttcaagggcc agccggccga cgtcgccgag gcctggagcg gggtcggcaa cagcggcttg
     4441 caggggcgga tccagcaact ggaccagatc ggcaagttga cccgccagaa ggaccccgcg
     4501 gtcgcgaagg tcgtcgatgc ggtgcaggcc gaactcgcga acgtgcagac caacaaggaa
     4561 ttggccgcga cgctcggcct ctcgcagtct ccgctgtatg cgatgtacgg cgttggcgac
     4621 gtgccggtaa tgcgggtcga actcgattcg cccgacacct tcaaggcgtt ctgggcgcgg
     4681 gtcgagaagc gcgccggcat caccgttccg accgcgactc tcgacaagca gagctattgg
     4741 gtaatcggcg gcgccggcgc gaaagtgcgc atcctggtcg cgatcgaagg caagcaactg
     4801 gtcgccaccg tcgccccggc caatgccggt cccgacatgc tgaagcaatt gcttggcctg
     4861 gccaagccgt ccagcaatgc gtcggatcgc ctgtccgacc tgaacggcaa gcagggctac
     4921 ggcgattacg gctcgggcta cgtcgacctt gccaggttgt tctccaacct gttcgacggc
     4981 aaggacgcga tcacccagga attcgccaag gacctgggcg gctcgctggc caatccctcc
     5041 tgcgccagcg aattcgcctc gctcgcgcaa caggttccga tggtcagcgc gggcgtcaaa
     5101 tcggccaccg cacaaaaggt gagcctgagc atcgacgcac ggttgtcgcc gtcgcttctc
     5161 ggtgcactta cggcgctcaa gcatcaggtg cccggcatgg gcgaggcgtc ggacacgtcg
     5221 atgttcgaca tggtgctggc gctgccgctg acgcaatggc aggcgttcct gcagggccgc
     5281 gccaaggcag cggcggggaa gacctaccgg tgcccggccc tgcaatcgct caacaggttc
     5341 gcgcaaaccg ccgcccatcc gccggtgcag atgccgccgg aagcggcctc gctgatgggc
     5401 tttcgcatcg tgctcgacca gtgggaagcc ggcccgcaga tcgccgggcg cctgctggtc
     5461 gcgagttccg atccggccgc actggcgcag aagatccagc agaccctgcc gcagttcgcg
     5521 ctgaagccga tcccgaccaa tggccagcca gtcgcgttcg acctgccgcc caggctcagg
     5581 gccctgctcg gtggcggcag ccagggctgg atcgcggcca atgacaaggc gctggcgatt
     5641 ggcgtcggcg acgacgagca aacgaagctg gccggcacgc tgaccgcttc gaccggcaac
     5701 ggcgaccggc tgttccgcat gcacttcgac ggcaagatgt acggcgtgct cggaagctgg
     5761 atggaccgct tctccgcgat ggcgccggcc gcgagccaag cccagttgca gcagcaggtc
     5821 gccacgttcc agcaaatggg caagatggtc gcgtcggtgg acgccgacgg gcggctcgac
     5881 ggcgacggcc tgcacttcga catcgacgtg acgcaccgct gagccgggaa accggggtca
     5941 gagtgcgctt tcacgagcca ctccgcgttg ctcacggagg tacggtttga aagtgcactc
     6001 tgaccccggt tttcgctctg accccggtct gccggttttt acaagcggtg gccgcgctcg
     6061 atggtttcgg tgagctcgac gtcgccgagg ccctgcgaca gcgaatgcgc gtcgccgccc
     6121 tgcgccagct tgatcgcgag gcgcacctcg ttggacgaat cggcgtagcg cagcgcatcc
     6181 tcgtagctga tgttgcctgc gtggtacagc tcgacaaggc tctggtcgaa ggtgcacatg
     6241 ccgaggttgg tggactgctt catcacgtcc ttgatcttgt ggatctcgcc cttgcggatg
     6301 tagtcctgga ccagcggggt ccccagcatg acctccatcg cgacccgacg gccgcgg
//
LOCUS       DASSNH010000214         6914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_440736, whole genome shotgun sequence.
ACCESSION   DASSNH010000214 DASSNH010000000
VERSION     DASSNH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440736"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="rsfS"
                     /locus_tag="VFK00_07760"
     CDS             <1..207
                     /gene="rsfS"
                     /locus_tag="VFK00_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7592881.1"
                     /translation="ADEVVKFAKRAGMLPLGVEGEDEAEWVLVDLGDIIVHVMLPRIR
                     EFYGLERLWTVGDQGTDAEAARAG"
     gene            220..690
                     /gene="rlmH"
                     /locus_tag="VFK00_07765"
     CDS             220..690
                     /gene="rlmH"
                     /locus_tag="VFK00_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7592882.1"
                     /translation="MRARLYAIGERMPRWVADGFAEYAKRLSRDLPLELVEIKPGARG
                     KGRDDARATADEGTSLLAALPRDAHVVALDGHGAAWSSEQLAGQLSNWRMAGRDLALL
                     IGGPDGHANEVLQRADQRWSLGPLTLPHMLVRLVVAEQLYRAVTILNGHPYHRG"
     gene            947..2767
                     /locus_tag="VFK00_07770"
     CDS             947..2767
                     /locus_tag="VFK00_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET7592883.1"
                     /translation="MSIDLGVVLTGLLVVGFLAQWLAWRMRLPAILFLLLIGIVLGPA
                     SGFFKPDRALGGFLFPSVSLAVALILFEGSLTLRFSELRGIGHAVRGMVSYGAVLALL
                     MLAAAAHWIAGVRWEVAFLFGALTCVTGPTVITPMLRTLRPTARIASALRWEGIVLDP
                     LGALFAVLVFEAIITHQQGHSLAIFAETIGIGAGIGLVVAWGLGFLLRRQMIPEYLQA
                     YGTLIAVLGAFTLANQFAHESGLVAVTIMGITLGNLRRVHIENIMDFKEHLSTVLVSM
                     LFVILAARLQWPLPPGMLMAGILVFVAAQFVIRPVSILVATFGSQLDWRERALLSYVS
                     PRGVVAASVSSLFALRLEDLGVTGAGALVPLVFILIIATVVLQSATARPLAYWLGVAA
                     PDPTGVLIYGSDEVARSVGKALAEQQFQVVLADDDWGGISAARMAGLTTFYGNPTSQH
                     AAMHLDLTGIGRLLAMSWRREMNSLACMHFRQEFGRERVYRLRVLAPEESGNRAAFAG
                     SLSAKALFGDDMTHTRFRDLLEAGWRIKANALTNTFDWPAFVAQHGADTVLLFGIEPG
                     GKLRVASNRRKTEPRSGWTVIALVPPAASGTTPAATAAAA"
     gene            2795..3367
                     /locus_tag="VFK00_07775"
     CDS             2795..3367
                     /locus_tag="VFK00_07775"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7592884.1"
                     /translation="MLYLASQSPRRRELLARLGVRFDVVDVDVPEVRAPGESPRAYVE
                     RVARAKAQAGLRAVLARDPDAWVLGADTEVVLGDRVFGKPADADAAAAMLRDLSGRTH
                     TVISIVCCATPLEMNCAACESSVTFAPLDDAGISDYVFTGESFGKAGGYAIQGRAAAF
                     VAHIAGSYSGVMGLPLFETAGLLRRTGGEP"
     gene            3523..5004
                     /gene="rng"
                     /locus_tag="VFK00_07780"
     CDS             3523..5004
                     /gene="rng"
                     /locus_tag="VFK00_07780"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HET7592885.1"
                     /translation="MTEEILINVTPREARVGVVENGMLQEVHLERTGHRGYVGNIYCG
                     TVRRVMPGMQAAFVDIGLERSAFLHAADTARPGLAADPVDAAAPPAAAVPPITELVHE
                     GQRVIVQVIKDPIGTKGARLSTYLSIPSRYLVLLPDSTTLGVSVRIEDEPERERLRAI
                     LAALAEGSGAGLIARTNAEGLEQQELAEDVAYVRKVWQSVREAIANAKPGERVYEEPP
                     LPLRVLRDLMHDDIEKVRVDSRETFERTLLFAQRFMPGLAGRVEHYPGERPIFDLYGV
                     EDEIQKALRKEVPLKSGGYLVIDQTEAMTTIDVNTGGYLGSRNLEETAFRTNLEAAQA
                     AARQLRLRNLGGIIIIDFIDMHDDGHKRQVLRMLEKAVARDHAKTTVYDMSALGLVEM
                     TRKRTTESLARQLCEPCPACSGRGIVKTVETVGYEIFREITRAVRQFDAGKLLVLAAP
                     TVVARMLEEESAAVAELEAFIGKSIRFQPEEEYGQEQFDVVLL"
     gene            5188..>6914
                     /locus_tag="VFK00_07785"
     CDS             5188..>6914
                     /locus_tag="VFK00_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HET7592886.1"
                     /translation="MTSAWRHHLRRARFAATAAVAGLLIALAVAIGLVQLLLPLATHY
                     PGFIASQLSGRLHRPVTFAAVSSEWQPSGPLLKVRGLTLGPGHPGGSSITLPHAAVKF
                     DFGAWLHPAHRWITLRLNDMKLRVEHSTSGWQVVGFSSAPGGEHASLQSLPVDLDLGN
                     LHLDIVDQVGHHAWELFAPRLRVVNIGDAVRFGGSVQQSGTRQALAISGRIQMATRDY
                     ALHLSTSDLNLAEASRGVDLHGYAVSSGHADLEAWGSWHAGKLASASVRYNMHGLVAS
                     GPDGRSLNLPLLAGVVRANRIDGGWELAWRGPGKPGADIDGAGGAVVQVRGKPGAWRV
                     SAAARSIDLSPWLSLLAMAPQAPKALVDWTVDAQPHAEIDRAAVVWNQGGDYRATVRF
                     SGLRAAASGAIPGVALARGTIRADDQALSLELPPQAATLAVTDVFRKPFSFTRFGGTL
                     VAWRADGAWNIALDSLRFVDAEGLAGNGRAHLVWRGNGHAPFLSAYAVLDHGQVTDAK
                     RFLPYHRMPQAMVAWMDRALVAGKITSARVLMRGDLDDWPFLDHQGRFEATATVADAA
                     FDFSNEWPQA"
BASE COUNT         1022 a         2241 c         2478 g         1173 t
ORIGIN      
        1 gccgacgaag tcgtcaagtt cgccaagcgc gcgggcatgt tgccgctcgg cgtcgagggc
       61 gaggacgaag ccgaatgggt gctggtcgat ctcggcgaca tcatcgtcca cgtgatgctg
      121 ccgcgcatcc gcgagttcta cggactcgag cgcctctgga cggtgggcga ccagggaacg
      181 gacgcggaag cagcccgcgc cggctgagta ccgtcggcgg tgcgcgcgcg gctttacgcg
      241 atcggtgaac gcatgccgcg atgggtcgcg gacggctttg ccgaatacgc gaagcggctg
      301 tcgcgcgacc tgccgctgga actcgtcgaa atcaagcccg gcgcgcgcgg caagggccgc
      361 gacgacgcgc gcgcaaccgc cgatgagggt acgtcgttgc tggccgcgct gccgcgcgac
      421 gcgcacgtcg tcgcactcga cggccacggc gccgcgtggt ccagcgagca gctcgcgggt
      481 cagttgtcga actggcgcat ggcgggtcgc gatctcgcgc tcctgatcgg cgggcccgac
      541 ggccatgcca acgaagtact gcaacgcgcc gaccagcgct ggtcactggg accgctcacg
      601 ctcccgcaca tgctggtgcg gctggtcgtg gccgaacagt tgtaccgcgc ggtgacgatc
      661 ctcaacggcc acccgtatca ccgcggttga ttgtcgttcc attcgtcgtg gacatccttg
      721 ccgcctccct gcacgaacag tagctggcgt ttttcccgca tcggttgcct cctggtgttg
      781 ccctgggcaa ttcaatgtag aagcgcgcca tgcgcgcgac tcgtggcaaa ggtggtcgcc
      841 gggcacggcc cgctcctgcg agagcggggc gaatgaaggt ccttgctcca cacggcactt
      901 ccagcgttgg ttaccatgag ccggacctcg aaggacgcga gattgcatgt cgatcgacct
      961 gggcgtggtg ctcaccgggc tgctggtggt cggtttcctg gcccagtggc tggcttggcg
     1021 gatgcgcttg ccggcgatcc tgttcctgtt gctgatcggg atcgtgctgg gtcccgcgag
     1081 cgggttcttc aagcccgaca gggcgctcgg tggcttcctg ttcccatcgg tgtcgctggc
     1141 ggtcgcgctg atcctgttcg aaggcagcct gacgctgcgg ttttcggagc tgcgcgggat
     1201 cggacatgcg gtgcgcggca tggtcagtta tggcgcggtg ctggcgttgt tgatgctggc
     1261 cgcggcggcg cactggatcg ccggcgtgcg ctgggaagtc gcgttcctgt tcggggcgtt
     1321 gacctgcgtg accggcccga ccgtgatcac cccgatgctg cgcaccttgc ggcccaccgc
     1381 gcggattgcc agcgccttgc gctgggaggg catcgtgctg gacccgcttg gtgctttgtt
     1441 cgccgtgttg gtgttcgagg cgatcatcac ccaccagcag ggccattcgc tcgccatctt
     1501 cgcggagacc atcggaatcg gcgcgggcat cgggctggtg gtggcttggg gactggggtt
     1561 cctcctgcgc cggcagatga tccccgagta cctgcaggcc tacggaacgc tgatcgcggt
     1621 gctgggcgca ttcaccctgg ccaaccagtt cgcgcacgag tcgggcctgg tcgcggtcac
     1681 gatcatggga atcacgctcg gcaacctgcg ccgggtccac atcgaaaaca tcatggactt
     1741 caaggagcac ctgtccacgg tgctggtgtc gatgttgttc gtgatcctgg cggccaggct
     1801 gcagtggccg ctgccgcccg gcatgttgat ggcggggatc ctggtgttcg tggctgcgca
     1861 attcgtgatc cgcccggtgt cgatcctggt ggcgaccttc ggcagccagc tcgactggcg
     1921 cgagcgcgcg ttgttgtcgt acgtgtcgcc gcgcggcgtg gtcgcggcgt cggtgtcgtc
     1981 gctgttcgcg ctgcggctcg aggatctcgg ggtgaccggc gccggggcgc tggtgccgct
     2041 ggtcttcatc ctgatcattg cgacggtggt gctgcagagc gcgacggcgc gccctctggc
     2101 gtactggctc ggcgtcgccg cgcccgaccc gaccggcgtg ctgatctacg gttccgacga
     2161 agtcgcgcgc agcgtcggca aggcgctggc ggagcagcag ttccaggtgg tgctggccga
     2221 cgatgactgg ggcgggatca gcgccgcacg catggcgggc ctcacgacgt tctacggcaa
     2281 cccgacttcc cagcacgccg cgatgcacct ggacctcacc ggcatcgggc ggttgctggc
     2341 gatgtcctgg cgtcgcgaga tgaactcgct ggcgtgcatg cacttccggc aggaattcgg
     2401 ccgcgagcgg gtgtatcggc tgcgggtgct ggcgccggag gagagcggca accgcgccgc
     2461 gttcgccggc agcctcagtg ccaaggcgtt gttcggcgac gacatgaccc atacacgctt
     2521 tcgcgatttg ctggaggcgg gctggcgcat caaggccaat gcgctgacca acaccttcga
     2581 ctggccggca ttcgtggccc agcacggcgc ggacaccgtg ctgctgttcg gcatcgaacc
     2641 gggcggcaag ttgcgggtcg cgagcaaccg gcgcaagacc gaaccgcgca gcggctggac
     2701 ggtgatcgcg ctcgtaccgc ccgctgcgtc aggcacgacc ccggcggcca ccgcggccgc
     2761 ggcctgagga cagtccgccc attatgctgg cccgatgctt tatctcgcct cgcaatcgcc
     2821 gcgccgacgc gagctgctcg cccggctcgg cgtccgcttc gacgtcgtgg acgtggacgt
     2881 tccggaggtt cgtgcgccgg gagagtcgcc gcgcgcgtac gtcgagcggg tggcgcgcgc
     2941 aaaggcgcag gcgggattgc gggccgtgtt ggcgcgcgat cccgacgcgt gggtactggg
     3001 cgcagacacc gaagtcgtgc tgggcgatcg ggtattcggc aagccggctg acgccgatgc
     3061 cgcggcggcg atgctccgcg acctctcggg tcgcacgcac acggtgatct cgatcgtctg
     3121 ctgtgccact ccgctggaaa tgaactgtgc cgcatgcgag tcgtcggtaa cgttcgcgcc
     3181 gctcgacgac gccggcatca gcgactacgt cttcaccggc gagtcgttcg gcaaggccgg
     3241 cggctatgcg atccagggca gggcggccgc attcgtcgcg catatcgcgg gcagctattc
     3301 cggcgtgatg ggactgccgc tgttcgagac ggcgggcctg cttcgccgga ccggtggcga
     3361 accatgacgg gcactgaatc agcgcggtgg ttgcatcgac agtcgtggcc gtgggccgct
     3421 ataagggtga gccggagctt gcgagcgcgc gcacgcgtag gtgcgggccg gcaatcgctt
     3481 catggatgac tgcttgcaga aaccccactt cgagcgcccg acatgaccga ggaaatcctg
     3541 atcaacgtca cgccgcgcga ggcacgggtc ggcgtggtcg agaacggcat gctgcaggaa
     3601 gtgcacctcg agcgcaccgg gcaccggggc tatgtcggga acatctactg cggcacggtg
     3661 cggcgcgtga tgccgggcat gcaggcggcc ttcgtcgaca ttggcctgga gcgcagcgcc
     3721 ttcctgcacg cagccgacac ggcgcggccg gggcttgccg ccgatccagt cgatgccgcc
     3781 gcgccgcccg cggcggccgt gccgccgatc accgagctgg tccacgaggg ccagcgggtg
     3841 atcgtgcagg tcatcaagga cccgatcggc accaaggggg cgcgcctttc cacgtatctc
     3901 tccattccct cgcgctacct ggtgctgctg cctgacagca ccacgttggg cgtgtcggtg
     3961 cgcatcgagg acgaaccgga gcgcgagcgt ttgcgtgcca tcctcgcggc gctggccgag
     4021 ggcagcggcg cggggctgat cgcgcgcacc aacgccgaag gcctcgaaca gcaggagctt
     4081 gcggaggacg tggcctacgt acgcaaggtc tggcaatcgg tgcgcgaagc gatcgcgaac
     4141 gccaagcccg gcgagcgggt ctacgaggag ccgccgctgc cgctgcgcgt gctgcgcgac
     4201 ctgatgcacg acgacatcga gaaggtgcgc gtggactcgc gcgaaacctt cgagcgcacg
     4261 ctgctgttcg cccagcgctt catgccgggt cttgccggac gcgtcgagca ttacccgggc
     4321 gagcggccga tcttcgacct gtacggcgtc gaggacgaaa tccagaaggc gttgcgcaag
     4381 gaggtgccgc tgaagtccgg cgggtacctc gtgatcgacc agaccgaggc gatgaccacg
     4441 atcgatgtca acaccggcgg ctacctcggc tcgcgcaacc tcgaggaaac cgcgtttcgg
     4501 accaacctcg aggccgcgca ggcggccgcg cggcaactcc ggctgcgcaa tctgggcggc
     4561 atcatcatca tcgatttcat cgacatgcac gacgacggcc acaagcgcca ggtgctgcgg
     4621 atgctcgaaa aggcggtcgc gcgcgaccac gccaagacta ccgtctacga catgagtgcg
     4681 ctggggctgg tcgagatgac ccgcaagcgc accaccgaga gcctcgcccg gcaactgtgc
     4741 gagccgtgcc cggcgtgcag cggccgcggc atcgtcaaga cggtcgaaac cgtcggttat
     4801 gaaatcttcc gcgagattac ccgggcggtg cggcagttcg atgccggcaa gctactggtg
     4861 ctggccgcgc ccaccgtggt ggcacggatg ctggaggagg aatccgcggc ggtcgcggag
     4921 ctggaagcgt tcatcggcaa gtcgatccgc ttccagccgg aggaggagta cgggcaggag
     4981 cagttcgatg tggttttgtt gtaagagccg ggaccgggga cccgggaccg gggacccgcc
     5041 gaaggcggtg ccggcgatcg tggttttggc aggttgggtt gaccccggac gcagtccggg
     5101 ggaagcccaa cggactcgcc gcatgatgtt gggcttcacc cggatggaat ccgggttcaa
     5161 cccaacctac atcgcgggcg cgatcgcatg acgagcgcct ggcgccatca tttgcgccgc
     5221 gcgcgatttg ccgcgaccgc ggctgttgcg gggttgttga tcgcgctcgc tgtcgccatc
     5281 gggctggtgc aactgctgtt gccgctggcg acccactacc cgggtttcat cgcgtcgcaa
     5341 ctgtcggggc gcttgcatcg gccggtcacg ttcgccgcgg tcagcagcga atggcagccg
     5401 tcgggtccct tgctgaaggt gcgcggcttg acactcgggc ccgggcaccc cggtggcagc
     5461 tcgatcacct tgccgcacgc ggcggtgaaa ttcgatttcg gcgcatggct gcatccggcc
     5521 catcgctgga tcacgctgcg cctcaacgac atgaagctgc gcgtcgaaca ctcgacgagc
     5581 ggatggcagg tcgtcggttt cagcagcgcc ccgggcggcg agcacgcatc gttgcaatcg
     5641 ctgccggtcg acctcgacct gggcaacctg cacctcgaca tcgtcgacca ggtcggccac
     5701 cacgcatggg agttgttcgc gccacggctg cgcgttgtca acataggcga cgccgtgcgc
     5761 ttcggcggca gcgtgcaaca gtccggtacc cggcaggcgc tggccatcag cggtcgcatc
     5821 cagatggcga cgcgcgatta cgcgctgcat ctgtcgacca gcgacctcaa cctcgccgag
     5881 gcgtcgcgcg gcgtggacct gcacggctat gcggtgagca gcggccacgc tgacctcgag
     5941 gcgtggggca gttggcacgc gggcaagctg gcgtcggcat ccgtgcgcta caacatgcat
     6001 ggcctggtcg cgtcgggccc ggacgggcgc agcctgaacc tgccgttgct cgcgggtgtc
     6061 gttcgcgcca accgcatcga cggcggctgg gaactggcat ggcgcgggcc gggaaagccg
     6121 ggtgcggaca tcgatggggc cgggggcgcg gttgtgcagg tgcgtggaaa acctggcgca
     6181 tggcgcgtat ccgcggccgc gcgttcgatc gatctttccc cgtggctctc gttgctggcc
     6241 atggcgccgc aggcaccgaa ggcgctcgtc gactggacgg ttgacgcgca accgcacgcg
     6301 gaaatcgaca gggccgcggt ggtgtggaac cagggcggcg actatcgcgc gacggtgcgg
     6361 ttctccggcc ttcgggcggc agcttcgggc gcgatacccg gggtcgcgct ggcacgcggt
     6421 acgatccgtg cagacgacca ggcgctctcg ctggagttgc cgccgcaggc ggcgacgctc
     6481 gcagtgaccg atgtgttccg caagcccttc tcgttcacgc ggttcggtgg cacgctggtg
     6541 gcatggcgcg cggacggcgc atggaacatc gcgctcgatt cgctccgctt cgtcgatgcc
     6601 gaggggctcg cgggcaacgg gcgtgcccac ctggtgtggc gcggcaacgg acacgcgccg
     6661 ttcctttccg cgtacgcggt gctcgaccac ggacaagtga cggatgcgaa gcggttcctg
     6721 ccctaccacc gcatgccgca ggcgatggtg gcgtggatgg atcgcgcgct cgtcgcgggg
     6781 aagatcacct ccgcgcgcgt cctgatgcgc ggcgacctgg acgactggcc cttcctcgac
     6841 catcagggcc gcttcgaagc caccgctacg gtggcggatg cggcgttcga cttttccaac
     6901 gaatggccgc aggc
//
LOCUS       DASSNH010000215         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_886843, whole genome shotgun sequence.
ACCESSION   DASSNH010000215 DASSNH010000000
VERSION     DASSNH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886843"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFK00_07790"
     CDS             <1..372
                     /locus_tag="VFK00_07790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7592887.1"
                     /translation="GFDAETPRLLIGHTWYARSRQRSPVNTACKLLLIGYAFERLGCA
                     AVAWETDILNAVSQRAIERLGAHRDGVLRQHRRRHDGTIRDTVAYSMLASEWPAARVR
                     LATRLERPLGDGSRTRRPAST"
     gene            complement(427..1152)
                     /locus_tag="VFK00_07795"
     CDS             complement(427..1152)
                     /locus_tag="VFK00_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7592888.1"
                     /translation="MKTNGYLAGLVAAVALFASAQASATLIDFEDAPAKSLSDNDAVT
                     NQYLGDGVTFTGAYLERTFQRGNPDANPQGFYNDQTHHWDEGSAASPGLGDWFIRSND
                     DIGSRGGQGVYLSIDYTAPVFAASGQIWDIDGGPRGTEQWRVVAYLNGVQVAYEDSPL
                     GDSHGAGSLNGLPWTFNLSSAGGFDSIEFVFIGSKTDSLGLAFDNFNTDALVGVPEPA
                     ALGMFGLGALLIGLFAGLRRRLD"
     gene            complement(1327..3888)
                     /gene="plsB"
                     /locus_tag="VFK00_07800"
     CDS             complement(1327..3888)
                     /gene="plsB"
                     /locus_tag="VFK00_07800"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HET7592889.1"
                     /translation="MRPDHYQPPLRNMARVMNQTLSRDALVHAGWWRALWGWLLGPWI
                     RIKRDPAEPVALLAAGTPVIYVIERNGFSDSLILERACREAGLPSPLAMVPGLGRRRR
                     AMFAMRGTGGWFQRRGRDHAAQGTIRQLLRMLEADPNRNVQLMPVAIYVGRAPNRQSG
                     WFSVLFSENWAVVGHFRRLLAMLLNGRDTIVRFSPPISLAELREESDERPERLQRKLA
                     RVLRVHFRRVRTAVIGPDLSHRRTVVDSVLNAEAVRAAIAATAAKENISTAKAWKRAE
                     HYMREIAADYSPPFVRSMSFLLANFWNKLYDGITMHHFDTLRANAPGHEIVYVPCHRS
                     HADYVLMSFQLHQSGVVVPHISAGANLNLPVLGPLLRRGGAFFQRRSFKGNALYSVIF
                     KAYLAQLIDRGVPIEYFIEGTRSRTGRLLAPRGGLLSMTVRAFLRAPRRPILFQPVYI
                     GYEKLMEGRAYIGELSGRKKEAESWWGLLRGLRALRQRYGRVALNFGEPVLLNPLLDA
                     VEPAWREQAGGENKPEWLSTVTDTLATRIQVEINRAADVNPVNLLALALLASPRHALA
                     ETDLLAQLDLMKAILTELPYSPRTTVTPMAPADIVAYGEQMGWITRVAHPLGDVLAVH
                     GEKAVLLSYFRNNVLHLFAAGAWVSCCFINNRRIARETIKRLGRAVYPFIRAELFLPW
                     DTDGFEARIDALIEFLVSRGLLASGGDGRLLHQASGREDNSYQLRLLAHCLLQAFERY
                     YIAIAALVKNGPHTLSAGQLENLCQLAAQRLSLLYQQSAPEFFDKSLFRGFIQKLRER
                     RIIWADEDGKLDFDAVLNDVARDARLILAREVRQSIMKITPGAVEAVSPAGTHAG"
     gene            complement(3977..4891)
                     /locus_tag="VFK00_07805"
     CDS             complement(3977..4891)
                     /locus_tag="VFK00_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592890.1"
                     /translation="MTCLFALAACHQAPPPPSNVTPEKAVATNLRLTAAGNFDDLMRN
                     RLPAADYATWRSEWEKAHAHPLPASVAQQEQFAKIMQMLTEPGAEAKLAKRLQPQLAR
                     KHGDKGDTMPILGGIVEAAGKQMIANSPQLGSAQRTLVSQAFDAAMAWARSTDFSDAG
                     KAKQAIDLACATARALHVETLEQWRALDYAGTMERYGVLWRALEQLLDIYGLDLAKVL
                     NDASVKTIGHEGNRATVEVQMTLAGKALSARWPMVKQEGHWYDEAMLEAWRKAHPVPA
                     PGGSTAPAAGGSAAPVAPPVSAPAASHT"
     gene            complement(5035..5238)
                     /locus_tag="VFK00_07810"
     CDS             complement(5035..5238)
                     /locus_tag="VFK00_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592891.1"
                     /translation="MGGVWHIFAFIHERGFVAVVPNHGDRYTIDTRTAIHGPTFHNSR
                     SGQAFSRPDMKLMRVVREPASMA"
     gene            5237..6265
                     /locus_tag="VFK00_07815"
     CDS             5237..6265
                     /locus_tag="VFK00_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592892.1"
                     /translation="MNEIRYPTLRAFRHTATASYLAAAAALLLVMFLHLLPALIAGLL
                     VYAVVDALAPLLERKLPGELSRQVVVGVLAVVVAGLLALLIFGVVAFFRAELGDPNTL
                     FDRLMPTIDRARIQLPAWITAYLPVSAEDFRATATQLARDHSVQLQVAGKETVHVFGR
                     ILVGLALGALVALTQARNGAGAGPLRRDLSARCRFFVRAFHDIVFAQARISALNTLFT
                     AIFLVVALPLAGVHLPFTKTLIVITFIVGLLPVIGNLISNALIAIVALSVSLWVAVGA
                     LVFLVLIHKLEYFLNARIVGGRIHARAWEILLAMLVMEAAFGLPGVVAAPIYYAYLKS
                     ELTAARLI"
BASE COUNT         1003 a         2171 c         2164 g         1111 t
ORIGIN      
        1 ggcttcgacg cggaaacccc gcgcctcctg atcggccata cgtggtacgc gcgcagccgc
       61 cagcgcagtc cggtgaatac cgcctgcaaa ctgctgctga tcggctacgc cttcgagcgg
      121 cttggctgcg ccgcggtcgc ctgggaaacc gacatcctca acgccgtgtc tcagcgcgcg
      181 atcgaacgac tcggggcaca tcgcgacggg gtgctgcggc aacacaggcg gcgccacgac
      241 ggcacgatcc gggacactgt cgcctacagc atgctcgcct cggaatggcc ggcggcacgt
      301 gtccgtctcg ccacccggct ggagcgtccg cttggcgatg gcagccgcac ccgccgaccc
      361 gcctccacat gaagaaagcg gctccgtctc cgggagccgc gggtcagtcc agcctcgccg
      421 ggcggctcag tccaaacgcc ggcgcaggcc cgcgaacagg ccgatcaaca gcgcgccgag
      481 cccgaacatt ccgagtgctg ccggctccgg cacgccgaca agggcgtccg tgttgaagtt
      541 gtcgaacgcc agtccgaggc tgtcggtctt cgagccgatg aatacgaact ctatgctgtc
      601 gaacccgccg gcgctcgaca ggttgaacgt ccagggcagc ccgttcagcg aaccggctcc
      661 gtgggagtcc cccagcgggc tgtcctcata ggcgacctgg acgccgttga ggtacgcgac
      721 gacccgccac tgctccgtgc ccctgggccc gccgtcgata tcccagatct ggccggacgc
      781 cgcaaagacc ggtgccgtgt agtcgatgct caggtacacg ccttggccgc cgcggctccc
      841 gatatcgtca ttggaacgga tgaaccaatc gccgaggccg ggcgacgccg cggagccttc
      901 gtcccagtgg tgcgtctggt cgttgtagaa gccctgcggg ttggcgtcgg ggttgcctct
      961 ctggaaggtc cgttcgaggt aggcgccggt gaaggtgaca ccgtcgccga ggtattgatt
     1021 ggtcaccgca tcgttgtccg acaacgattt ggccggtgcg tcctcgaagt cgatcaaggt
     1081 ggccgaagcc tgcgccgacg cgaacagcgc cacggctgcc acgaggcccg ccaagtatcc
     1141 gttcgttttc atgaccctgc ccccggcatc gagtgttcgt cgaccgcaag ccaccgatga
     1201 aacagcattt cgcgtgccaa gcaaaaaaca gcaatccggt caatcgcttc gctttgcggt
     1261 ctttcgcgcc ccctgcctac tttgtaaatg ccgccgacac ttcggcgaag tcgggcgcgg
     1321 cgcgggtcag cctgcgtggg tccccgccgg ggataccgcc tcgactgcgc cgggcgtgat
     1381 cttcatgatc gactggcgga cctcgcgggc gaggatcaac cgcgcgtcgc gggcgacgtc
     1441 gttgagtacc gcgtcgaaat cgagcttgcc gtcttcgtct gcccagatga tgcgacgttc
     1501 gcgcagtttc tggatgaagc cgcggaacag gctcttgtcg aagaattccg gcgcgctctg
     1561 ctggtagagc agcgagagcc gttgcgcggc gagttggcag aggttttcga gctgtccggc
     1621 gctcagcgta tgcggaccat tcttcaccag ggccgcgatg gcgatgtagt agcgctcgaa
     1681 tgcctgcagc aggcagtgtg ccagcaggcg caactggtag gaattgtctt cgcggccgct
     1741 cgcctggtgc aggagccgcc cgtcgccgcc gctcgccagc agtccgcgcg agaccaggaa
     1801 ttcgatcagc gcgtcgatcc gcgcctcgaa accatccgtg tcccacggca ggaacaactc
     1861 cgcccggatg aacggataca cggcacgccc aaggcgcttg atcgtctcgc gcgcgatgcg
     1921 ccggttgttg atgaagcagc acgacaccca cgcaccggcg gcgaacagat gcagcacgtt
     1981 gttgcggaag taggaaagca gcaccgcctt ctcgccgtgc accgcgagca cgtcgccgag
     2041 cgggtgcgcg acgcgcgtga tccagcccat ctgttcaccg taggcgacga tgtcggcggg
     2101 tgccatcggc gtgacggtag tgcggggaga gtacggcagc tcggtcagga tcgccttcat
     2161 cagatccagt tgcgctagca ggtccgtctc ggccagcgca tggcgcggcg aggccagcaa
     2221 cgccagtgcg agcaggttca ccggattgac gtcggccgcg cggttgatct cgacctggat
     2281 gcgggtggcg agggtgtcgg tgacggtgga cagccattcc ggcttgtttt caccgccggc
     2341 ctgttcccgc cacgccggtt ccaccgcgtc gagcaacggg ttcagcagca ccggctcgcc
     2401 gaaattgagc gcgacccgtc cgtagcgctg gcgcagcgcg cgcagcccgc gcagcaggcc
     2461 ccaccacgat tccgcttcct ttttccggcc ggacagttcg ccgatgtagg cgcgaccttc
     2521 catcaacttt tcgtagccga tgtagaccgg ctggaacagg atcggccggc gcggcgcgcg
     2581 caggaacgcg cgcacggtca tcgaaagcag tcccccacgc ggcgccagca gccgccccgt
     2641 ccgcgagcgg gtgccttcga tgaaatactc gatcggaacc ccacggtcga tgagctgcgc
     2701 caggtacgcc ttgaagatca ccgaatacag cgcgttgccc ttgaagctgc ggcgctggaa
     2761 gaacgcgccg ccgcggcgca gcaacggacc cagcacgggc aggttgagat tggcgccggc
     2821 ggagatgtgc ggcaccacga ccccgctctg gtggagctgg aacgacatca gcacgtaatc
     2881 ggcgtggctg cggtggcacg gcacgtacac gatttcgtgg ccgggcgcgt tggcgcgcag
     2941 cgtgtcgaag tggtgcatcg tgatgccgtc gtacagcttg ttccagaagt tcgcgagcag
     3001 gaacgacatc gatcgcacga acggcggcga atagtccgcc gcgatttcgc gcatgtagtg
     3061 ctcggcgcgt ttccaggcct tggccgtgct gatgttctcc ttggcggcgg tggcggcgat
     3121 cgccgcacgc accgcttccg cgttcagcac cgagtccacc accgtgcggc gatgcgaaag
     3181 gtcggggccg atcaccgcgg tgcggacgcg ccggaagtgc acgcgcaaca cccgcgcgag
     3241 cttgcgctgc aaccgttcgg gccgctcgtc cgattcctcg cgcagctcgg cgagcgaaat
     3301 cggcggcgag aaccgcacga tcgtgtcgcg cccgttcaac agcatcgcga gcagccgccg
     3361 gaaatggccg accaccgccc agttttccga gaacagcacg ctgaaccagc cggattgccg
     3421 gttcggtgcg cggcccacgt agatcgcgac cggcatcagt tgcacattgc ggttcgggtc
     3481 ggcttccagc atccgcagca gttgccggat cgtgccttgt gcggcgtggt cgcggccgcg
     3541 ccgctggaac cagccaccgg ttccgcgcat cgcgaacatc gcgcgacgac ggcggccgag
     3601 tcccggcacc atcgcgagcg ggctgggcag cccggcttcg cggcacgcgc gctcgaggat
     3661 caggctgtcg gaaaagccat tgcgctcgat cacgtagatc accggcgtgc ctgcggcgag
     3721 cagcgccacg ggttcggccg gatcgcgctt gatccggatc cacggcccca gcagccatcc
     3781 ccacaaggcg cgccaccaac cggcatgcac gagcgcgtcg cgggacaggg tctggttcat
     3841 gacgcgggcc atgttgcgca gtggcggctg gtagtggtcg gggcgcacga atcgggattg
     3901 tcccacaacc ccgccgctcg ctcgtgcggc cacctgggcg ccggcctacg gaaaggggcc
     3961 agcggcccgg acaagatcag gtatgcgaag ccgcgggggc gctgacaggc ggcgccacag
     4021 gcgcggccga acctccggct gccggcgcgg tgctgccacc cggcgcaggc acggggtgcg
     4081 ccttgcgcca tgcctccagc atcgcctcgt cgtaccaatg cccttcctgt ttcaccatcg
     4141 gccatcgggc cgacaacgcc ttgccggcga gggtcatctg cacctcgacc gtcgcccggt
     4201 tgccctcgtg gccaatggtc ttgacgctgg cgtcgttcag caccttggcg aggtcgagcc
     4261 cgtagatgtc gaggagttgc tcgagcgccc gccagaggac cccgtagcgc tccatggtcc
     4321 ccgcgtagtc gagcgcgcgc cattgctcca gcgtctcgac gtgcaacgcc cgggcggtgg
     4381 cgcacgccag gtcgatcgcc tgcttcgcct ttccggcgtc gctgaaatcg gtggacctgg
     4441 cccaggccat cgcggcgtcg aacgcctggc tcacgagggt ccgttgcgcg gagccgagct
     4501 gcggcgagtt cgcgatcatc tgcttgccgg cggcttccac gataccgccg aggatcggca
     4561 tggtatcgcc cttgtcgccg tgcttgcgcg ccagttgtgg ctgcagccgc ttggcgagct
     4621 tcgcctcggc accgggttcg gtgagcatct gcatgatctt cgcgaactgc tcctgctgcg
     4681 cgaccgaggc cggcagcggg tgcgcatggg ccttttccca ctcgctgcgc caggtggcgt
     4741 agtccgcggc cggcaggcga ttgcgcatca ggtcgtcgaa gttgcccgcc gccgtgaggc
     4801 gcaggttggt ggcgacggcc ttctcgggcg tgacgttgct cgggggtggc ggcgcctggt
     4861 ggcaggccgc cagtgcgaac aggcaggtca cgacgatcgc ggggattcgc cggaccggcc
     4921 ggcgacgtgt tccggtttgc atgcggacgg agtgtggcga ggccgcgccg gataggcaag
     4981 ccaccaccca ttgagcccaa actcgagcat cggtcggagc gccttttgcg gaagtcaggc
     5041 catggatgcc ggttcgcgca cgacacgcat gagcttcatg tctggccgcg agaacgcttg
     5101 cccggagcgg ctgttgtgga aagtcgggcc atggatggcc gttctggtgt cgatggtgta
     5161 tcgatcgcca tgattgggga caactgcaac gaagccgcgt tcatggatga acgcaaagat
     5221 atgccagact ccgcccatga acgagatccg ctaccccacg ctgcgcgcgt tccggcatac
     5281 cgcgaccgcg agttatctcg ccgccgccgc cgccttgctg ctggtgatgt tcctgcacct
     5341 gttgcccgcc ctgatcgccg gcctgctggt gtacgcggtc gtcgatgcgc tggcgccgct
     5401 gctcgaacgg aagttgcccg gcgagctgtc gcggcaggtg gtggtcgggg tgctggcggt
     5461 ggtggtcgcg gggctgctgg cgttgctgat cttcggcgtc gtcgcgttct tccgcgccga
     5521 gctcggcgat cccaacacct tgttcgaccg gctgatgccg accatcgacc gcgcacgcat
     5581 ccagctccct gcctggatca cggcctacct gccggtcagc gcggaggatt tccgtgccac
     5641 cgccacgcaa ctcgcgcgcg atcactcggt ccaactgcag gtcgccggca aggaaaccgt
     5701 gcacgtattc gggcgcatcc tggtcggcct cgcattgggc gccctggtgg cgttgacgca
     5761 agcccgcaac ggcgccggtg ccggcccgtt gcggcgcgac ctgtccgccc gctgccggtt
     5821 tttcgtgcgg gcattccacg acatcgtgtt cgcgcaagcc cgcatctcgg cattgaacac
     5881 cctgttcacc gcgatcttcc tcgtggtcgc gctgccgctg gccggcgtgc acctgccgtt
     5941 caccaagacc ctgatcgtga tcacgttcat cgtcgggctg ctgccggtga tcgggaacct
     6001 gatttccaac gcgctgatcg cgatcgtggc gctgtcggtg tcgttgtggg tggcggtcgg
     6061 tgcgctggtg ttcctggtgc tgatccacaa gctcgagtac ttcctgaatg cgcgaatcgt
     6121 gggcggccgg atccatgcac gcgcctggga gatcctcttg gcgatgctgg tgatggaagc
     6181 cgcgttcggc ttgcccggcg tcgtcgcggc accgatctac tatgcgtacc tcaagagcga
     6241 actgaccgcg gcgcgcctga tttgaatatg cgcacgcagg cgccggcacg cgaaccgcac
     6301 cggccggagc cggcgcgcac caaagttata ctcgcccgct gccggcttcg aaccgcgcca
     6361 tgctgcgatt cctgtatacg ctcgcgatgt atctcgtcac gccggtcatc gtggcccgcc
     6421 tggtggtgca cggcgtccgc taccggagc
//
LOCUS       DASSNH010000216         4358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_519043, whole genome shotgun sequence.
ACCESSION   DASSNH010000216 DASSNH010000000
VERSION     DASSNH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519043"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="VFK00_07820"
     CDS             <1..1663
                     /locus_tag="VFK00_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET7592893.1"
                     /translation="VRARHEEMCAFMACAHAKFTGEVGVCMATSGPGAVHLLNGLYDA
                     KLDHQPVLAIVGQQARTALGGHYQQEVDLPNLFKDVASEFVEMATVPAQIRHLVDRAM
                     RIAMSKKTVTCLIIPNDLQTMEAVETPPRAHGTIHSGMGYRAPRIVPKHEDLEQAAEL
                     LNSGKKVAILVGAGALGATDEVMQVADMLGAGVAKALLGKAVLPDELPYVTGAIGLLG
                     TKPSWDLMTGCDTFLMIGSRFPYSEFLPKEGQARAVQIDIDPKMLSIRYPMEINLVGD
                     SAATLRELIPLLKRKEDRSWREKIEKGVAEWWQVLEARAMHDADPINPERVFWEASPR
                     LPDNCILSSDSGSAANWYARDLKIRRGMMASLSGGLATMGPGVPYVIGAKFAFPDRVP
                     IALVGDGAMQMNGNAELITIADYWKEWSDPRLVIVVLNNRDLNQVTWEERVQEGDPRY
                     DAAQHVPDFPYARYAEMVGLKGILVNQPEQIGPAFDAAFAADRPCVIEAITDPEVPPL
                     PPHITFKQAKAFVESMLKGDSKTPAIIRESAREMMASIFPGSKGG"
     gene            1666..2757
                     /locus_tag="VFK00_07825"
     CDS             1666..2757
                     /locus_tag="VFK00_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7592894.1"
                     /translation="MQVVALSAPRVSVYKVPTDSPESDGTLEWDSTMLVLVEIEAGGK
                     SGLGYSYADTATAALIRDKLAGVVEHRDAFDIPGAWMAMVHAIRNLGRPGIASMAISA
                     IDNALWDLKARLLDLPLCKLLGMVRECAPVYGSGGFTSYDERQLRDQLGDWAKHGFGM
                     VKMKVGREQQRDPERVQAARKAIGDRVQLFVDANGAYSRKQALRMAQTFAESDVAWFE
                     EPVSSDDRDGLRLLRDRVPATMDIAAGEYGYDSWYFRRMLEAGAVDVLQADATRCAGI
                     TGFMQASALCAAFQVPLSGHCAPSIHAHPACAAPPLRHIEYFHDHVRIESMLFDGALP
                     ARDGALHPDLSRPGLGLEFKRADANRYAI"
     gene            3050..4156
                     /locus_tag="VFK00_07830"
     CDS             3050..4156
                     /locus_tag="VFK00_07830"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7592895.1"
                     /translation="MAVAHDGTGKAGSPFAGKVVVVTGASAGVGRAAALAFAKQGARV
                     ALIARGEESLESARREIELAGGRSLAIMADVADAKAVEAAADKVEVELGPIDIWVNNA
                     MVTVFAPVSEIEPEEFKRVTEVTYLGGVYGTMAALKHMRRRDRGTIVQVSSALAHRSI
                     PLQSAYCGAKHALVGFVDSLRSELIHEGSGIHLTSVQMPALNTPQFGWARNKMPKRPQ
                     PVPPIFQPEVAANAILYAASHNRRNVPVGSSTWKAQWGQKFIPGLLDRYLGRTAYKGQ
                     QSDEADPHDRPDNLFSPVAGDPGTHGRFDARAKASSPALWVEEHRGPILGGAALLGML
                     AALGWYGARSRSAPARVVRHGIHKVSRNLRNLRP"
     gene            4153..>4358
                     /locus_tag="VFK00_07835"
     CDS             4153..>4358
                     /locus_tag="VFK00_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HET7592896.1"
                     /translation="MSLGQTLWVIPEGYIPPDSEEQKGSRFVSHEAACLLNANDRDAH
                     VAITLYFVEKEPVGPYKVRVPARRT"
BASE COUNT          773 a         1403 c         1495 g          687 t
ORIGIN      
        1 ggttcgcgcg cgccatgagg aaatgtgcgc cttcatggcc tgcgcgcacg cgaagttcac
       61 cggtgaagtc ggcgtatgca tggcaacttc gggccccggt gcggtacatc tcctgaatgg
      121 cctctacgac gccaagctcg atcaccagcc ggtgctcgcg atcgtcggac agcaggcgcg
      181 cacggccctc ggcggacact accagcagga ggtcgacctc ccgaacctgt tcaaggatgt
      241 cgcgagcgaa ttcgtggaaa tggcgacggt gcccgcgcag atccggcacc tggtcgaccg
      301 ggcgatgcgg atcgcgatgt cgaagaagac cgttacctgc ctgatcattc cgaacgacct
      361 gcagacgatg gaggcggtgg aaacgccgcc gcgcgcccat ggcaccatcc attccggcat
      421 gggctatcgg gcgccgcgga tcgtgccgaa gcacgaggat ctcgagcagg cggccgagtt
      481 gctgaacagc ggcaagaagg tcgcgatcct ggtcggggcc ggcgcactcg gcgccaccga
      541 cgaagtgatg caggtggccg acatgctggg cgcgggcgtc gccaaggcgc tgctcggcaa
      601 ggccgtgctg ccggacgagc tgccgtacgt caccggggcg atcggcctgc tcggcaccaa
      661 gccgagctgg gacctgatga ccggctgtga tacgttcctg atgatcggtt cgcgcttccc
      721 gtattcggaa ttcctgccca aggaaggcca ggcacgcgcg gtccagatcg acatcgatcc
      781 gaagatgctg tcgatccgct atccgatgga gatcaacctg gtcggcgatt ccgccgccac
      841 cttgcgcgaa ctgatcccgc tgctcaagcg caaggaggac cgttcctggc gcgaaaagat
      901 cgagaagggc gtcgccgaat ggtggcaggt gctcgaggcg cgggcgatgc acgacgcgga
      961 cccgatcaat ccggaacgcg tgttctggga ggcctccccg cggctgcctg acaactgcat
     1021 cctgtcgtcg gactcgggtt ccgcggccaa ctggtacgcg cgcgacctca agatccggcg
     1081 cgggatgatg gcctcgctgt ccggcggcct cgccacgatg ggcccgggcg tgccctacgt
     1141 gatcggcgcc aagttcgcgt tcccggatcg cgtgccgatc gcgctggtcg gcgacggcgc
     1201 gatgcagatg aacggcaacg cggaactgat cacgatcgcc gactactgga aggaatggtc
     1261 ggatccgcgg ctcgtgatcg tggtgctcaa caaccgcgac ctcaaccagg tgacctggga
     1321 agaacgcgtg caggagggcg acccgcggta cgacgccgcg cagcacgtgc cggatttccc
     1381 ttacgcgcgc tacgccgaga tggtggggct gaagggaatc ctcgtgaacc agcccgagca
     1441 gatcggcccg gcgttcgacg cggccttcgc ggccgaccgg ccgtgcgtga tcgaggcgat
     1501 caccgacccc gaggttccgc cgctgccgcc gcacatcact ttcaagcagg ccaaggcgtt
     1561 cgtcgagtcg atgctgaagg gcgactccaa gacgccggcg atcatccggg aatccgcgcg
     1621 cgaaatgatg gccagcatct ttccgggaag taaaggtgga tgacaatgca agtggtcgcg
     1681 ctgtccgccc cgcgcgtctc ggtctacaag gtgccgactg attcgccgga gtccgatggc
     1741 accctggagt gggacagtac gatgctcgtg ctggtcgaaa tcgaggcggg tggcaaaagc
     1801 ggactcgggt acagctacgc cgacaccgcc acggccgcgc tgatccgcga caagctggcc
     1861 ggcgtcgtcg aacaccgcga cgcgttcgac attcccggtg cgtggatggc gatggtccat
     1921 gccatccgca atctcgggcg gcccggcatc gcctcgatgg cgatcagcgc aatcgacaat
     1981 gcactttggg atttgaaggc gcgcttgctc gacctgccgc tctgcaagtt gctcggcatg
     2041 gtgcgcgagt gcgcgccggt gtatggcagc ggcgggttca cctcctacga cgagcggcaa
     2101 ttgcgcgacc agctcggcga ctgggccaaa cacggcttcg gcatggtcaa gatgaaggtc
     2161 ggccgggagc agcagcgcga cccggaacgg gtgcaggcgg cacgcaaggc gatcggtgac
     2221 cgcgtgcagc tcttcgtcga tgccaacggc gcgtattcgc gcaagcaggc tttgcgcatg
     2281 gcgcagacct tcgccgaatc cgatgtcgcc tggttcgagg agccggtctc ctcggacgat
     2341 cgcgacggcc tgcggctgct gcgcgatcgt gtgcccgcca cgatggacat cgcggccggc
     2401 gaatacggct acgactcgtg gtatttccgg cggatgctgg aggcgggcgc cgtggacgtg
     2461 ctgcaggccg atgccacccg ctgcgccggg atcacgggct tcatgcaggc ctcggccttg
     2521 tgcgcggcgt tccaggtgcc gttgtcagga cattgcgcgc cttcgatcca tgcgcacccc
     2581 gcctgcgcgg cgccgccgct gcgccacatc gaatatttcc atgaccacgt ccgcatcgaa
     2641 agcatgctgt tcgacggcgc cctgccggcc agggacggcg cgctgcatcc ggatctttcg
     2701 cggccggggc tggggctcga attcaagcgc gccgatgcga atcggtacgc gatttgacgt
     2761 ttcgggaaca agcaggcaag ccaggaggag cgcagccatg caagtcgatc atctggacag
     2821 caaaccgcag tgcaggcacc aagggtgcaa ttgcagcgtg ccctcgggcg aggaattctg
     2881 cagcgagcac tgccgcaacg ccgaggcgag gggaaacgcc agtgaaggcc gcggttcatg
     2941 cggctgcggc catcctgagt gcaacaacag cgcgcccgca ccgggcagtt gatggcaagg
     3001 gccacatgtt ccggcgccgg tcgcatacgg aaaagcatgg aggaatggaa tggctgtagc
     3061 tcatgacgga acaggcaaag ccggttcgcc gttcgccggc aaggtcgtgg tggtgaccgg
     3121 tgcgtcggca ggcgtcggcc gcgccgcggc gctcgcattc gcgaagcagg gcgcgcgggt
     3181 ggcgctgatc gcccgcggcg aggaatcgct agagtcggcg cggcgcgaga tcgaactcgc
     3241 cggcggcagg tcgctcgcga tcatggcgga cgtggccgac gcgaaggcgg tcgaggccgc
     3301 ggccgacaag gtcgaagtcg agctcggccc gatcgacatc tgggtcaaca acgcgatggt
     3361 cacggtcttc gcgccggtca gcgagatcga accggaggaa ttcaagcgcg tcaccgaggt
     3421 cacctacctc ggcggcgtgt acggcacgat ggcggcgctc aagcacatgc ggcggcgcga
     3481 tcgcggcacg atcgtacagg tcagttcggc gctcgcccat cgcagcattc cattgcagtc
     3541 ggcgtattgc ggcgccaagc atgcgctggt cggattcgtc gattcgctgc gttcggaact
     3601 gatccacgag ggcagcggca tccatctcac cagcgtgcag atgccggcac tcaacacgcc
     3661 gcaattcggc tgggcccgca acaagatgcc caagcgtccg caaccggttc cgccgatctt
     3721 ccagcccgag gttgccgcca atgcgatcct gtacgcggcc agccacaacc gccgcaacgt
     3781 tccggtcggg tcctcgacct ggaaggcgca atggggccag aaattcatcc ccggcctgct
     3841 cgaccgctac ctgggccgca ccgcgtacaa gggtcagcag agtgacgagg ccgacccgca
     3901 cgaccgaccg gacaacctct tctcgccggt cgccggcgat ccggggaccc acggtcgttt
     3961 cgacgcccgg gccaaggcca gcagtccggc gctgtgggtc gaggaacatc gcggaccaat
     4021 cctcggcggc gcggcgttgc tgggcatgct ggcggcgctc ggttggtatg gcgcgcgctc
     4081 gcgctccgcg cccgcgcggg tggtgcgcca cgggatccac aaggtttcac gcaacctgcg
     4141 gaacctgcgg ccatgagcct cggtcaaacg ctgtgggtaa ttccggaggg ctacattccg
     4201 ccggattcgg aggaacagaa gggctcgcgt ttcgtcagcc acgaagcggc ctgcctgttg
     4261 aacgccaacg atcgggacgc gcacgtggcg atcaccctgt atttcgtcga aaaggagccg
     4321 gtcggtccct acaaggtccg ggttccggcc cgtcgcac
//
LOCUS       DASSNH010000217         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_423391, whole genome shotgun sequence.
ACCESSION   DASSNH010000217 DASSNH010000000
VERSION     DASSNH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423391"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFK00_07840"
     CDS             <1..423
                     /locus_tag="VFK00_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET7592897.1"
                     /translation="VRGMQQQMAQRRMMDAVPKADVVVMNPSHFAVALRYDGDNMRAP
                     RVVAKGLDLVAQRIRGIATQHDVPLVTAPPLARALYHTTRIGQEIPAALYAAVAQILA
                     YVYRVQQAAAHDGDVPPTPNPEVDPQLLGPYQMPEGEH"
     gene            426..2525
                     /gene="flhA"
                     /locus_tag="VFK00_07845"
     CDS             426..2525
                     /gene="flhA"
                     /locus_tag="VFK00_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HET7592898.1"
                     /translation="MAAVPTLMQQFGRIGRRGIGAPIIMLVLLAMVVLPLPPFMLDVL
                     FSFNIALSLVIVLAVVYVMRPLEFGAFPTVVLGATLLRLALNIASTRVVLLHGHNGPG
                     AAGKVIEAFGEFVIGGNFTVGLVVFSILTIINFVVITKGATRVSEVTARFTLDSMPGK
                     QMAIDADLNAGLLTQEQARERRQEVREEADFYGSMDGASKFVRGDATAGILILLINIV
                     GGFAVGVLQHGLSAGEAAHTYTLLTIGDGLVAQIPALLLSVATAVIVTRVSRAQDMGK
                     QVLSQVFGQPRALAVTAVVLGVMGVIPGMPNVAFLLLGGICGGAAWMLDRRRRDAEKA
                     ARDRANAAADAEPAPAAERVELSWDDVAAVDTIGLEVGYRLIPLVDRNQGGELLGRIK
                     SVRRKLSQDLGFLVPPVHIRDNLDLGPSHYRISLLGVPVGEAEVHNERLLAINPGRVH
                     GRLDGIATRDPAFGLEAVWIEPGLREHAQTLDYTVVDAATVIATHLSHVLQAHAHELL
                     GHQEVQQLLDRLSAEAPKLVEDLVPKRLSLGVVVKVLQNLLAEHVPIKNMRGIVESLA
                     EHAGQSQDSGALTAQVRVALGRQIVQEISGLGSEVPVITLAPELEQILLQSLQGGGPA
                     GAAVEPGLAERLQQNVAEAAQRQEMAGEPAVLLVAPQLRTWLARFTRHVSPGLHVLAY
                     NEVPDNRRVRLVSALGR"
     gene            2693..>2814
                     /locus_tag="VFK00_07850"
     CDS             2693..>2814
                     /locus_tag="VFK00_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET7592899.1"
                     /translation="MKIKRFVAPAMRQAMQQVRDDQGPDAVILSTRRCEEGIEI"
BASE COUNT          457 a          959 c          965 g          433 t
ORIGIN      
        1 gtgcgcggca tgcagcagca gatggcgcag cgccggatga tggatgcggt gccgaaggcc
       61 gacgtggtgg tgatgaaccc cagccacttc gcggtggcgc tgcgctacga cggcgacaac
      121 atgcgcgcgc cgcgcgtggt cgccaagggc ctggacctcg tggcccagcg catccgcggc
      181 atcgccacgc agcacgacgt cccgctggtg accgcgccgc cgcttgcccg cgcgctgtac
      241 cacaccaccc gcatcggcca ggaaatcccg gccgcgttgt acgccgcggt cgcgcagatc
      301 ctggcgtacg tctatcgcgt acagcaggcc gccgcccacg acggcgacgt ccccccaacg
      361 ccgaatcccg aggtcgatcc gcagttgctc gggccctacc agatgcctga aggggagcac
      421 tgacgatggc cgccgtcccc acactcatgc aacagttcgg ccgcatcggc cggcgcggaa
      481 tcggcgcgcc gatcatcatg ctggtactgc tggcgatggt ggtactgccg ctgccgccgt
      541 tcatgctgga cgtgcttttc agcttcaaca tcgcgctgtc gctcgtgatc gtgctggcgg
      601 tggtgtacgt gatgcgtccg ctggagttcg gcgcgttccc gaccgtggtg ctgggcgcga
      661 ccctgttgcg gctcgcactc aacatcgcct ccacgcgcgt ggtgctgctg cacggccaca
      721 acggcccggg cgccgccggc aaggtgatcg aggcgttcgg cgagttcgtg atcggcggca
      781 acttcacggt cggcctggtg gtgttctcga tcctgacgat catcaacttc gtggtgatca
      841 ccaagggcgc gacccgcgtg tcggaggtga ccgcgcgctt caccctggat tcgatgcccg
      901 gcaagcagat ggcgatcgac gccgacttga acgccgggct cctgacgcag gaacaggcgc
      961 gcgagcgccg ccaggaagtc cgcgaggaag cggacttcta cggctcgatg gacggtgcct
     1021 cgaagttcgt gcgcggtgac gcgaccgccg gcatcctgat cctcctgatc aacatcgtcg
     1081 gcggcttcgc ggtcggcgtg ctgcagcacg gcctgtctgc cggcgaggca gcgcacacct
     1141 acaccctgct gacgatcggc gacggcctgg tcgctcagat cccggccctt ctgctgtcgg
     1201 tcgccacggc cgtgatcgtg acccgcgtgt cgcgcgcgca ggacatgggc aagcaggtgc
     1261 tgtcgcaggt gttcggccag ccgcgggcgc tcgcggtcac cgcggtggtg ctcggcgtga
     1321 tgggcgtgat ccccggcatg ccgaacgtcg cgttcctgct gctgggcggc atctgcggcg
     1381 gcgccgcgtg gatgctggac cgtcgtcgtc gcgatgccga aaaggccgcg cgcgaccgcg
     1441 ccaatgcagc cgccgacgcg gagccggcgc cggccgcgga gagggtcgag ctttcgtggg
     1501 acgacgtcgc cgccgtggac acgatcggcc tcgaagtcgg ctaccggctg atcccgctgg
     1561 tcgaccgcaa ccagggcggc gagctgctgg gccgcatcaa gtcggtgcga cggaagcttt
     1621 cgcaggacct cggattcctg gtgccgccgg tgcacatccg cgacaacctc gatctcgggc
     1681 ccagccacta tcggatcagt ctcctgggcg tgccggtcgg cgaggccgaa gtgcacaacg
     1741 agcgcctgct cgcgatcaat cccggccgcg tgcacggccg tctcgacggc atcgccacgc
     1801 gcgatcccgc gttcgggctg gaagccgtgt ggatcgaacc cggcctgcgc gaacacgcgc
     1861 agacactcga ctacaccgtg gtcgacgcgg ccaccgtcat cgccacccat ctgtcgcacg
     1921 tgctgcaggc acacgcccac gaactgcttg gccaccagga ggtgcagcaa ctgctcgacc
     1981 ggctttccgc cgaggccccc aagctggtcg aagacctggt gcccaagcgc ctgtcgctgg
     2041 gcgtggtggt caaagtgttg cagaaccttt tggccgagca cgtgcccatc aagaacatgc
     2101 gcggcatcgt cgaatcgttg gcggagcacg ccgggcagag ccaggattcc ggcgcgctca
     2161 ccgcgcaggt gcgcgtcgcg ctgggccgcc agatcgtcca ggaaatcagt ggcttgggaa
     2221 gcgaggtccc ggtgatcacg ctggccccgg agctggaaca gatattgctt cagtccctcc
     2281 agggcggcgg gcccgcaggt gccgccgtcg agccgggact ggccgagcgg ctgcagcaga
     2341 acgttgccga agccgcgcaa cgccaggaga tggccggcga gccggcggtg ctgctggtgg
     2401 caccccagct caggacgtgg ctggcgcgct tcacgcgcca tgtctcgccc gggttgcacg
     2461 tgctggccta caacgaggtg ccggacaacc gccgcgtgcg actggtctcg gcgctggggc
     2521 gatagagccg aagagcaggg aacagggagc ggggaacggg gaacagggaa cagagacaac
     2581 cgaacacgag cgccgccctc ccggttcccg gttcccgctt ttcacaacgg aacatcgaaa
     2641 tgcacacgcc acgtaacact tcgaaattca acacgccgcg gagtcatcaa ccatgaagat
     2701 caagcgattc gtggcaccgg cgatgcgcca ggccatgcag caggtccgcg acgaccaggg
     2761 ccccgatgcg gtgatcctgt ccacgcggcg ctgcgaggaa ggcatcgaga tcat
//
LOCUS       DASSNH010000218         7049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_192720, whole genome shotgun sequence.
ACCESSION   DASSNH010000218 DASSNH010000000
VERSION     DASSNH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192720"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFK00_07855"
     CDS             <1..211
                     /locus_tag="VFK00_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7592900.1"
                     /translation="ELVDEQLVEKRRGLGMFVTEGAHSALLKSEREHFLREEWPRLRA
                     RIERMGLDLDKLLAAPPHQPDKRDS"
     gene            211..1125
                     /locus_tag="VFK00_07860"
     CDS             211..1125
                     /locus_tag="VFK00_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7592901.1"
                     /translation="MNDVFEAVAERPQAAPEAGDTIIHAQGLTKRYKNAVALDEAEFE
                     VGPGRIVGLIGPNGAGKTTALKAILGLTDFEGDLKVLGMDPRRQRPQLMREVCFIADV
                     ATLPRWIRVHEAIDFVAGVHPRFDRAKCERFLARTKLSANQRVREMSKGMIVQLHLAI
                     VMAIDARLLVLDEPTLGLDILYRKQFYQNLLEDYFDENKTILITTHQVEEVEHILTDL
                     LFIRDGKIALSTTMDEMGARFAEVLVNADKVDAARALNPIDERAAVFGKSIFVYDSAD
                     PEQLAGLGEVKRPSVSDLFVATMKGTYA"
     gene            1122..2114
                     /locus_tag="VFK00_07865"
     CDS             1122..2114
                     /locus_tag="VFK00_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HET7592902.1"
                     /translation="MNAIVKSPFAWLLKREYWEERRGFLWAQIAAVAVILAITILGII
                     AGEVFRARMGGDMSVHMGAANLSQMLRSASAHDVGAVVKSLDMTLLLFGFISSVVLFF
                     VVFFYLLGALYDDRRDRSILFWKSLPVSDTATVISKVIAAVIIAPIIAVVVVLAGYVG
                     AQIIVSLWFLAHGVNPIGLLWAHSDPFSLWLHLLAMIPVNAVWALPTVGWLLVWSAAV
                     RSKPFLWAVVIPVIAGVLNFWIGLLGLPNIDTQFFWGNLVGRALLSVVPAGWIAPNEL
                     STHGINFVDGDQLSAISYSSMAHAFTLPGMWIGAAVGIGLIAVAIWFRRWRDEA"
     gene            2132..2794
                     /locus_tag="VFK00_07870"
     CDS             2132..2794
                     /locus_tag="VFK00_07870"
                     /inference="COORDINATES: protein motif:HMM:NF022551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 family protein"
                     /protein_id="HET7592903.1"
                     /translation="MNRILTFAGVVLVVVALAACSPGIHTLGNSVTMDSTGVVVHVAG
                     RPDAHVGRDGSLSIDGKIVAVTPDQRKLLQRYVQETSAAVEAGAKVGKQGISIAAHGI
                     GAAFASIFHDGPSPQEKKLDAESDQIEAAASKLCGHLKALGATQAELATEIPAFAPYA
                     SGDRPECKITHTTTHRIDGNKSTSVTYTSGDGTAASGKPAPSRHAASPGSPAASDESQ
                     QP"
     gene            2791..3420
                     /locus_tag="VFK00_07875"
     CDS             2791..3420
                     /locus_tag="VFK00_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592904.1"
                     /translation="MILQKPSILCIAASLAVLTACSAGQSPTTGQTTAAAASSTSQSI
                     AAAINRAMDKASVELATKNITVSNDDDTAPKAAITPQGDFLIAGKPVSLTPDQRKEML
                     AYRRQLIEIAKRGIAIGKQGAALGIHAASAAIAAAFSGESEQQVRQRVEAQTTSIRQA
                     AAQICDRLPALRDSQQKLASELPAFRPYADLTPAKIDECRTDALHDDKD"
     gene            3490..4086
                     /locus_tag="VFK00_07880"
     CDS             3490..4086
                     /locus_tag="VFK00_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592905.1"
                     /translation="MKLAHLARITALSCSLVLLTACTAFVSADHELDAASRQLATGKV
                     TLTAEADDVPDAAITPQGDFLVAGKAVALTPQQRREVSAYRAQYIAIARQGIAIGHEG
                     VDVGRRAVVPMVFAALSGASDDAIEARMKKRLAGVREATAKLCDRLPDLMAAQRQLAG
                     DLPAFAPYATLTQADIEDCRKDVADGFDVANARNDAGE"
     gene            4079..4798
                     /locus_tag="VFK00_07885"
     CDS             4079..4798
                     /locus_tag="VFK00_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET7592906.1"
                     /translation="MNELNRNARCAGLLYLLVILAAPVRLVYIPAKLFVSGDTGATIA
                     NVAAHETLFRFGIFCDLASGVLMLFLTLALHRLFANVDRKLATLMVILGGVLPAALYF
                     FNVLNDAAVLLIARGPDFLSVFDQPQRDALALLFLKLHGQEIGAAMVFWGLWLLPLAW
                     LVLRSGFLPKVFGWGLILNGLAYLAQSVTWSVLPQFDDIVTSVSMPLQLIEILFMLWL
                     LVLGARPTLRGRPVPFPATST"
     gene            4800..5273
                     /locus_tag="VFK00_07890"
     CDS             4800..5273
                     /locus_tag="VFK00_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HET7592907.1"
                     /translation="MPRTNALQHFDPPVQLKLSALWASVMLCYIYGDYFGLFVPGTLK
                     AMLEGQMGSLGPTTQGVLLGTSALITFPALMVALSLLLPPTVNRWLNILLGLVYTAIV
                     LFFMAGGWWFYEALAIVEIILQLLVVWHAWKWPKVPAIAGEDVPANPYRGATSAP"
     gene            complement(5343..6728)
                     /locus_tag="VFK00_07895"
     CDS             complement(5343..6728)
                     /locus_tag="VFK00_07895"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7592908.1"
                     /translation="MPNFRTEHDSMGELKVPADALWGAQTQRAVDNFPISGLTLPRDF
                     IRALGLIKVAAADANLKLGHLKSTQAAAIRKAAQRVAGGEFDSHFPIDIFQTGSGTSS
                     NMNANEVIAHIASQGGKAKIHPNDHVNYGQSSNDVIPTAIHVSASLAAREQLLPALKH
                     LQGTIDRRARGLKNVAKTGRTHLMDAMPVTFGQELSGWSAQVGSGIARISDALQRMQR
                     LPQGGTAVGTGINADPKFARAFCRELSKATKVRFTSMDNFFEGMASQDAAVELSGQLK
                     TLACSLMKISNDLRWMNSGPLAGLGEIELVALQPGSSIMPGKVNPVVPEAMTMVCAQV
                     IGNDATITIGGQAGNFQLNVMLPVVAYNLLQSITILANASRLLADKAIAGFTVNKQRV
                     DEALALNPILVTALNPVIGYEKGAATAKQAYKQKRPIIDVALETTGLSKAELKKLLDP
                     VALTRGGIHGK"
BASE COUNT         1198 a         2347 c         2212 g         1292 t
ORIGIN      
        1 ggaactggtc gacgagcaac tggtggagaa gcgccgcggc ctcggcatgt tcgtcaccga
       61 gggtgcccac tcggcgctcc tcaagagcga acgcgaacat ttcctgcgcg aagagtggcc
      121 gcgcctgcgc gcgcgcatcg agcgcatggg gctggacctc gacaagctgc tggcagctcc
      181 gccgcaccaa cctgacaaga gggattcgtg atgaatgacg ttttcgaagc ggtcgcggaa
      241 cggccccagg cggcaccgga agcgggcgac accatcatcc acgcgcaggg actgaccaag
      301 cgctacaaga acgcggtggc gctggacgag gccgagttcg aagtcgggcc tggccggatc
      361 gtgggcctga tcggtcccaa cggcgccggc aagaccaccg cgctcaaggc gatcctcggc
      421 ctcaccgact tcgagggcga cctcaaggtg ctgggcatgg acccgcgccg gcagcggccg
      481 caactgatgc gcgaggtgtg cttcatcgcc gacgtcgcca ccctgccccg ctggattcgc
      541 gtccacgagg ccatcgattt cgtcgccggc gtgcacccgc gcttcgaccg ggccaagtgc
      601 gagcgtttcc tcgcgcgcac caagctctcg gccaaccagc gcgtgcgtga aatgtccaag
      661 ggaatgatcg tgcagctcca cctcgcgatc gtgatggcca tcgacgcccg gctgctggta
      721 ctggatgaac cgaccctcgg cctggacatc ctgtaccgca agcagttcta ccagaacctg
      781 ctggaggact acttcgacga gaacaagacc atcctgatca ccacccacca ggtcgaggaa
      841 gtcgagcaca tcctcaccga cctgctgttc atccgggacg gcaagatcgc cctcagcacc
      901 acgatggacg agatgggcgc gcgcttcgcc gaggtgctgg tgaatgccga caaggtcgat
      961 gccgcccggg cgctgaatcc gatcgacgag cgcgcggcag tgttcggcaa gagcattttc
     1021 gtgtatgaca gcgcggatcc cgaacagctt gcgggcctcg gcgaggtcaa gcggccttcg
     1081 gtatccgact tgttcgtggc caccatgaaa gggacctacg catgaatgcg atcgtcaaat
     1141 cgcccttcgc ctggctgctc aagcgcgagt actgggagga gcgacgcggc ttcctgtggg
     1201 cccagatcgc cgcggtcgcg gtgatcctgg ccatcacgat cctcgggatc atcgccggcg
     1261 aggtattccg cgcacgaatg ggcggcgaca tgtccgtgca catgggtgcg gccaacctca
     1321 gccagatgct gcgcagcgcg tcggcgcacg atgtcggcgc cgtggtcaag agcctggaca
     1381 tgaccttgct gctgttcggc ttcatctcgt cggtggtgct gttcttcgtg gtgttcttct
     1441 acctgcttgg cgcgctctac gacgaccgtc gcgaccgcag catcctgttc tggaaatcgc
     1501 tgccggtgtc cgacacggcc accgtgatct ccaaggtcat cgcggccgtg atcatcgcgc
     1561 caatcatcgc ggttgtcgtg gtgctggccg ggtacgtcgg cgcacagatc atcgtgagcc
     1621 tctggttctt ggcgcacggc gtgaatccga tcgggctgct gtgggcgcac agcgacccgt
     1681 tctcgctgtg gctgcacctc ctcgcgatga ttcccgtgaa cgcggtgtgg gcgttgccga
     1741 cggtcggctg gctgctggtg tggtccgccg cggtccgcag caagccgttt ctctgggccg
     1801 tggtgatccc ggtgatcgcc ggcgtcctca atttctggat cggcctgctc ggtctgccca
     1861 acatcgacac ccaattcttc tggggcaacc tcgtggggcg cgccttgctc agcgtggtcc
     1921 cggccggctg gatcgcaccc aatgaactga gcacgcacgg catcaacttc gtggacggcg
     1981 accaattgtc ggcgatcagt tactcgtcga tggcgcacgc cttcaccctg cccggcatgt
     2041 ggatcggtgc cgcggtcggc ataggcctga tcgcggtcgc catctggttc cgccgctggc
     2101 gggacgaagc gtgacgacac gggagacagc catgaacagg atcctgacat tcgcaggcgt
     2161 ggttctggtg gtcgtggcgc tggccgcctg ctcgccgggt atccacacgc tcggcaacag
     2221 cgtcaccatg gactcgacgg gcgtcgtggt gcacgttgcg ggccgcccgg acgcgcacgt
     2281 cggccgggac ggcagcctca gcatcgacgg caagatcgtt gccgtgacgc cggaccagcg
     2341 gaaactgctg cagcgctatg tccaggaaac gagcgccgcc gtcgaagcag gcgcgaaggt
     2401 cggcaagcag ggaatctcga tcgccgcgca cggcatcggc gcggctttcg catccatttt
     2461 ccatgatggt ccctcgcccc aggagaaaaa gctggatgcc gagtccgacc agatcgaggc
     2521 cgcggcaagc aagttgtgcg gccacctcaa ggcgctcggc gccacgcagg cagaactcgc
     2581 caccgaaatc cccgcgttcg cgccctatgc gtcgggcgac aggcccgaat gcaagatcac
     2641 ccacaccacg acccaccgga tcgacggcaa caaatccacg tcggttactt acacatccgg
     2701 cgatggaacg gcggcctccg ggaaacctgc accgtcccgg cacgccgcca gccccggaag
     2761 ccccgccgct tctgacgaga gccagcagcc atgatcctgc aaaagccttc gatcctctgc
     2821 atcgcggcga gcctcgctgt cctcacggcc tgcagcgcgg gccagtcgcc gacgaccggc
     2881 cagacgaccg ctgccgcggc ttcgtccacc agccagtcga tcgcagccgc gatcaatcgg
     2941 gcgatggaca aggcgagcgt cgaactcgcg acgaagaaca tcaccgtctc caacgacgat
     3001 gacacggcgc cgaaagccgc aatcacgccg caaggcgatt tcctgatcgc gggtaaaccg
     3061 gtttcgctga cgcctgacca gcgcaaggaa atgctcgcct atcgccggca actcatcgaa
     3121 atcgccaagc gaggcatcgc catcggcaag cagggcgcgg ccctcggcat ccatgccgca
     3181 agcgctgcca tcgcggctgc gttttcgggc gaatccgagc agcaggtccg ccaacgcgtc
     3241 gaggcgcaga cgaccagcat ccgccaggcc gccgcacaaa tctgcgatcg tctgcccgcg
     3301 ttgcgggaca gccagcagaa actcgcctcc gaactgcctg ccttcaggcc gtacgcggac
     3361 cttacgccgg cgaagatcga cgaatgccgt acggacgcgc tgcacgacga caaagactga
     3421 catccagggg atgtcgcgtt gcggacgcgc atgtcgcgcc ggctcgagtc attcaacgag
     3481 gacactgcca tgaagcttgc ccatctcgcg cgcatcaccg ccctctcctg cagcctcgtg
     3541 ctgctcaccg cctgcacggc cttcgtcagt gccgaccatg agctcgacgc ggcctcgcgg
     3601 cagctcgcga ccggcaaagt gacgcttacc gccgaggcgg acgatgtccc ggacgccgcc
     3661 atcaccccgc aaggcgattt cctggtcgcc ggcaaggccg tcgcgctgac tccgcaacag
     3721 cgccgggagg tctcggctta ccgcgcccaa tacatcgcca tcgcgcgcca aggcattgcg
     3781 atcggccacg agggcgtcga tgtcggccgc cgcgccgtgg tgccgatggt gttcgccgcg
     3841 ttgtccggag cctcggacga cgcgatcgaa gcccgcatga aaaagcggct cgccggcgtg
     3901 cgcgaagcca ctgccaagtt gtgcgatcgt cttccggatc tgatggccgc gcagcggcaa
     3961 ctggctggcg atctccccgc atttgcgccc tacgcgacgc tcacgcaagc ggacatcgag
     4021 gactgccgca aggatgtggc cgacggtttc gatgtcgcca acgcgcggaa cgacgccggt
     4081 gaatgagttg aatcggaacg cgcgctgcgc tggcttgctg taccttctgg taatcctcgc
     4141 cgcaccggtc cgcctggttt acatcccggc gaagctgttc gtgtccggcg acaccggtgc
     4201 gaccatcgcg aatgtcgcgg cgcacgaaac gctgttccgc ttcggcatct tctgcgacct
     4261 ggcgtcgggc gtgctgatgc tctttctcac gctggcgctt cacaggttgt tcgcgaacgt
     4321 cgaccgcaag ctggcgacgc tgatggtgat tttgggcggc gtccttcccg cagcgctcta
     4381 cttcttcaac gtattgaatg acgccgcggt attgctgatc gcgcgcgggc cggatttcct
     4441 ctccgtattc gaccagccgc agcgcgatgc cttggccctg ctgttcctga aactgcatgg
     4501 tcaggaaatc ggcgccgcga tggtgttctg gggcttgtgg ctgctgcctt tggcgtggct
     4561 ggtgctgcgc tcgggattcc tgccgaaggt tttcggctgg gggctgatcc tcaatggatt
     4621 ggcctacctc gcgcaaagcg ttacctggtc ggtcttgcca caattcgacg atatcgtcac
     4681 cagcgtctcg atgccgctgc aactcatcga gatcctgttc atgttgtggc tgctcgtgtt
     4741 gggtgcgcgc cccaccttgc gcggccggcc ggtacctttc cccgccacga gtacctgaca
     4801 tgccacgaac caatgcactg caacacttcg atccgcccgt gcagttgaag ttgtccgcac
     4861 tgtgggcttc ggtgatgctt tgctacatct acggcgatta cttcgggctg ttcgtgccgg
     4921 gcacgctgaa ggccatgctc gaaggccaga tgggttcgct cggcccgacc acgcaaggcg
     4981 tcctgctcgg cacctccgcg ttgatcacgt tcccggcgtt gatggttgcg ctctcgttgt
     5041 tgttgccgcc aaccgtgaac cgctggctca acatcctgct cggactcgtc tacaccgcca
     5101 tcgtcctgtt tttcatggcg ggcggatggt ggttttacga agcgctggcc atcgtcgaaa
     5161 tcatcctgca actgctggtc gtctggcatg catggaagtg gccgaaagtg ccagcaatcg
     5221 cgggcgagga tgtgcctgcc aacccctacc gtggcgcaac ttcagcgccg tagggcaatc
     5281 ggcgggcatt gcccgcccta cttggtggcg tgcgcagccg caggcggcgg gcgatgcccg
     5341 tcctacttgc cgtggatgcc gcccctggtc aacgcgaccg gatcgagcag cttcttcaat
     5401 tccgccttgg acaagcctgt cgtttccagc gccacgtcga tgatcggacg cttctgcttg
     5461 taggcctgct tggcggtcgc ggcgcccttc tcgtatccga tcacggggtt cagcgcggtc
     5521 acgaggatcg ggttcagcgc cagtgcctcg tccacgcgct gcttgttgac cgtgaagccc
     5581 gcgatggcct tgtcggccag cagccgcgag gcgttcgcga ggatcgtgat cgactgcaac
     5641 aggttgtagg cgaccaccgg cagcatcacg ttcaactgga agttgccggc ctgccctccg
     5701 atcgtgatcg tggcgtcgtt gccgatcacc tgcgcgcaca ccatggtcat cgcttcgggc
     5761 accaccgggt tgaccttgcc cggcatgatc gacgaacccg gctgcagcgc gaccagctcg
     5821 atttcgccga gtcccgcgag tggcccggaa ttcatccagc gcaggtcgtt ggagatcttc
     5881 atcagcgaac acgccagcgt cttcaactgg cccgagagtt ccacggccgc atcctgcgat
     5941 gccatgccct cgaagaaatt gtccatcgag gtgaacctga ccttcgtcgc cttcgacaat
     6001 tcccggcaaa aggccctcgc gaacttcgga tcggcattga tgccggtgcc gaccgcggtg
     6061 ccgccctggg gcaggcgctg catccgctgc aaggcgtccg agatgcgtgc gatgccggaa
     6121 ccgacctgcg ccgaccagcc cgaaagttcc tgcccgaacg tcaccggcat cgcgtccatc
     6181 aagtgcgtgc ggccggtctt ggcgacgttc ttcaggccgc gcgcccgccg gtcgatggtc
     6241 ccctgcaggt gcttcagcgc cggcaacaac tgttcgcgcg ccgcgagcga agcactgacg
     6301 tggatcgcgg tcggaatgac gtcgttggag ctctgcccgt aattgacgtg gtcgttcggg
     6361 tggatcttcg ccttgccgcc ctgcgaggcg atgtgcgcga tcacctcgtt ggcattcatg
     6421 ttgctggagg tgcccgaccc ggtctggaaa atgtcgattg ggaagtgcga gtcgaactcg
     6481 ccgccggcga cgcgttgcgc ggccttgcgg atcgctgcgg cctgcgtgga cttcaggtga
     6541 ccgagcttca ggttggcatc cgccgccgcc accttgatga ggcccagcgc gcggatgaaa
     6601 tcgcgcggca aggtcagccc ggatatcggg aaattgtcca ccgcgcgctg ggtctgcgcg
     6661 ccccacagtg cgtctgcggg taccttgagt tcacccatgc tgtcatgctc ggtgcggaag
     6721 ttcggcattg cggaaaatcc agacaggaaa gccactcatt ataacgagca gctgacgcta
     6781 caatggcgca tcctccaccc gttcgccctg agcgtagcga tccacagatc gcgaagtcga
     6841 agggaaaacg gggtggctga gcgtttcgac tccgctcgcc ttgcgagcta cgctcaacga
     6901 acggggtaat ttcccatctc acaagggccg tccatgcaac tcgacccgct aaccgccctt
     6961 tcgccgctgg acggccgcta tgccgccaag gtggaggccc tgcgcccgat cttcagcgaa
     7021 tacggcctga tgcaccggcg cgttgcggt
//
LOCUS       DASSNH010000219         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1698373, whole genome shotgun sequence.
ACCESSION   DASSNH010000219 DASSNH010000000
VERSION     DASSNH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698373"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /gene="pcnB"
                     /locus_tag="VFK00_07900"
     CDS             complement(<1..1278)
                     /gene="pcnB"
                     /locus_tag="VFK00_07900"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET7592909.1"
                     /translation="MIPRDRHNVSRKQISRGALRVLYGLKDAGFRACLVGGAVRDLLL
                     ERQPKDFDVATDATPEDVRKLFRNCRLIGRRFRLAHVVFGPEIVEVATFRGTGDGTGS
                     GDRHVVNGRILRDNVWGTIEEDALRRDFRVNALYYDIADFSVIDFVGGMEDLEQKRLR
                     LIGDPATRYHEDPVRMLRAARLAAKLGFTIDPEAAAPFATLGHLLCDAPPARLFDECQ
                     KMFLAGYGLASFRTLVEYGLLEHLFPGTARALAHDESGSLRAMIERGLGGTDARVAER
                     RPVTPAFLFAVLLWGEVAQLAARLQAEGTVRSLAWQRAAHRVMAEQGARVAIPRRFAY
                     AIEEIWALQPRFAERTRKRVFRLMAHPRFRAAYDFLLMRADESEDLRESGEWWTRAQE
                     LSADELSASLGPKPAAGHAGEPAAKPKRRRRRGG"
     gene            1402..1477
                     /locus_tag="VFK00_07905"
     tRNA            1402..1477
                     /locus_tag="VFK00_07905"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1436..1438,aa:Glu,seq:ctc)
     gene            1686..2009
                     /gene="fdxA"
                     /locus_tag="VFK00_07910"
     CDS             1686..2009
                     /gene="fdxA"
                     /locus_tag="VFK00_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HET7592910.1"
                     /translation="MTFVVTDNCVKCKYTDCVEVCPVDCFHEGPNFLAIDPDECIDCT
                     LCEPECPANAIFPEDDVPAGQEHYIALNAELAKGWPVLTERKDPPPDAKEWDGKPDKL
                     PLLER"
     gene            complement(2123..2740)
                     /locus_tag="VFK00_07915"
     CDS             complement(2123..2740)
                     /locus_tag="VFK00_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592911.1"
                     /translation="MKRLPLLFFLLAPVALAGCQTIKAHNPFRHKEPAYKSAQQEQPL
                     EVPPGMDQPATSEALAIPGEEASAAAAAPAAGAPPMTSMAPPPAGVATAAQGAEGSTS
                     MVLADTPDSAYHRVGLALERGELGQVTAHDDAARTYQVAINTTVTKKEEGGFFHRLFH
                     RSKSETVAGDVTVTVAPQGNGSLVEAAGNPDAAARVIELLKQRLK"
     gene            complement(2758..3648)
                     /gene="dapA"
                     /locus_tag="VFK00_07920"
     CDS             complement(2758..3648)
                     /gene="dapA"
                     /locus_tag="VFK00_07920"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7592912.1"
                     /translation="MDIAGSLCAIATPFDASGALDLDAFARLVDHQLDGGTQGIVVAG
                     STGEAHMLDEGEYERLLAFAIERVAHRVPVIAGTGEAATAKTIALTRRARSLGADAAL
                     VVTPFYVRPTQDGLRRHFGEVAARCDIPVVMYNVPSRTGCDMQPETVAELRDAAGIAG
                     IKEAVATSERIRVIAQLQRPGFVYLSGDDGSAAEAMLAGAGGVVSVVTNLVPKRFRAL
                     CDAARGGDREATQRHASGIEPLLDALECAPNPIPVKAGLAELGLCGAALRLPLVELPP
                     GPERTRLRDVLVTLAGAAAP"
     gene            3832..>4214
                     /locus_tag="VFK00_07925"
     CDS             3832..>4214
                     /locus_tag="VFK00_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7592913.1"
                     /translation="MTVKPVTTLNRSSPRPAPGHQLLIQAVAPARQSPLLVLSRRIAD
                     AGCNLVESRVATVGADVSVLLLARGAWDAIAKLETALARIGRDENLHLLSYRTEARDE
                     QSHLLPYMVEVVAADKAGVLVELID"
BASE COUNT          651 a         1460 c         1426 g          677 t
ORIGIN      
        1 gccgccgcgc cgccgccggc gtttcggctt ggcggccggt tcgccggcat gacccgcggc
       61 cggcttgggg ccgagcgacg cggaaagttc gtcggccgaa agttcctgcg cccgggtcca
      121 ccattcgccc gattcgcgca ggtcttcgga ttcgtccgcg cgcatcaaca ggaaatcgta
      181 ggcggcgcgg aaccgtggat gcgccatcag ccggaaaacc cgcttgcggg tgcgctcggc
      241 gaagcgcggc tgcaatgccc agatttcctc gatcgcgtac gcgaagcggc gggggatcgc
      301 aacccgcgcg ccctgttcgg ccatgacccg gtgggccgcg cgttgccagg ccagcgaacg
      361 aaccgtgccc tccgcctgca gccgtgcggc gagctgcgcg acctcgcccc ataacaggac
      421 cgcgaacagg aaggccggcg tcaccggacg gcgctcggcg acgcgtgcat cggtgccgcc
      481 gaggccgcgc tcgatcatcg cgcgcaggct gcccgattcg tcgtgcgcca gcgcgcgcgc
      541 cgtaccgggg aacaggtgtt ccagcaggcc gtattccacc agcgtgcgga agctcgcgag
      601 gccgtagccc gcgaggaaca tcttctggca ttcgtcgaac agccgcgctg gcggcgcatc
      661 gcacagcagg tgtccgagtg tcgcgaacgg agcggccgct tcgggatcga tcgtgaaccc
      721 aagcttggcc gcgagccggg cagcgcgcag catccgcacc ggatcctcgt ggtagcgcgt
      781 ggcggggtcc ccgatcaggc gcagccgctt ctgctcgagg tcttccatgc caccgacgaa
      841 gtcgatcacg ctgaagtcgg cgatgtcgta atacagcgcg ttgacgcgga agtcgcggcg
      901 cagcgcgtcc tcctcgatcg tgccccacac gttgtcgcgc aggatccggc cgttcaccac
      961 gtggcggtca ccgctgccgg tgccgtcgcc ggtgccgcgg aaggtcgcga cctccacgat
     1021 ctccggcccg aacaccacgt gcgccaggcg gaaccggcgg ccgatcagcc ggcagttgcg
     1081 gaacagtttc ctgacgtctt ccggcgtggc gtctgtggcg acgtcgaaat ccttgggctg
     1141 gcgttccagc aggaggtcgc gcaccgcacc gccgacgagg caggcgcgga agccggcgtc
     1201 cttcagtccg tacaggacgc gcagcgcgcc gcggctgatc tgcttgcggg aaacgttgtg
     1261 ccggtcgcgc ggaatcacgc acgcgtcgga caggaggggg tcggatgctg tcgaatcgga
     1321 actcaaacgg ggacgtccat gcgaaatcgt ggccggatcc gggcacgaag tccgttatac
     1381 taccgcgctc cggtgcccga cgctcccttc gtctagtggc ccaggacatc gccctctcac
     1441 ggcggtaacg cgagttcgag tctcgcaggg agcgccagta tttgtgcaat cgtccctgat
     1501 tgctgctccg ttccggggcg atacgacgac acacgcgacc gccacctgtc acaccggaac
     1561 gggcttcctg ccctgacttt ccacgaaagc cgctcagggc gaagcgttca cggccgcttc
     1621 gattgaagcc gcatgtgtgc gatgatgtcg tgtcatcgca tactgattgc gagtccctgc
     1681 tcccaatgac cttcgtcgtc accgacaact gcgtgaagtg caaatacacc gactgcgtgg
     1741 aggtgtgtcc ggtcgattgc ttccatgaag gaccgaactt cctcgcgatc gatccggacg
     1801 agtgcatcga ctgcacgctt tgcgagccgg aatgcccggc caacgcgatc tttcccgagg
     1861 acgacgtgcc ggccggccag gaacactaca tcgcgctgaa cgccgaactg gcgaagggct
     1921 ggccggtgct taccgagcgc aaggatccgc cgcccgatgc caaggagtgg gacggcaagc
     1981 cggacaagtt gccgctgctg gaacggtaag cggcaagcgc gtcgccggac ggcgcgcgcc
     2041 tcccggtcca catgcggcgg gcagtgcccg ccttgcgatc ccggaaattc gaggcctggc
     2101 ggcgaaccgg atggtcgcct cgctacttca accgttgctt cagcagctcg atgacccgcg
     2161 ccgccgcgtc gggattgccg gccgcctcca ccaggctgcc gttgccctgc ggggcgacgg
     2221 tgacggtgac gtcaccggcc acggtttcgc tcttgctgcg gtggaacagg cggtggaaga
     2281 agccgccctc ttccttcttg gtcaccgtgg tgttgattgc gacctggtag gtgcgcgccg
     2341 cgtcgtcgtg cgccgtcacc tgccccagtt cgccacgctc cagcgccagg cccacccggt
     2401 ggtaggcgct gtcgggcgta tcggcaagca ccatgctggt gctgccttcg gcaccctgcg
     2461 cagcggttgc gacgcccgcg gggggcggtg ccatggacgt catcggcggc gcgcccgctg
     2521 ccggcgctgc ggccgcggca ctggcttcct cacccggaat cgcgagcgcc tcgctggtcg
     2581 ccggctggtc catgcccggt ggcacttcca gcggctgctc ctgttgcgcg gatttgtagg
     2641 ccggttcctt gtgcctgaac gggttgtggg ccttgatggt ctggcaaccc gccaaggcga
     2701 ccggcgcgag caggaaaaac agcaatggga gtcgtttcat gggcgcattg tgcatgatca
     2761 gggcgcggcg gcgccagcga gggtcaccag cacgtcgcgc aagcgggtgc gttctggtcc
     2821 cggcggcagt tcgaccaggg gcagccgcag cgcggcgccg cacagcccga gctccgcgag
     2881 cccggccttg accggtatcg gattgggcgc gcattcgagt gcatccagca gcggctcgat
     2941 ccccgacgcg tgtcgctgcg tcgcctcgcg atcgccgccg cgcgcggcat cgcacagggc
     3001 gcggaaccgc ttcgggacca ggttggtcac caccgagacc acgcctcccg cgccggccag
     3061 catcgcctcg gctgcgctgc catcgtcgcc cgatagatag acaaaacccg ggcgctggag
     3121 ttgcgcgatc acgcggatcc gctcggaagt cgccacggct tccttgattc cggcgatgcc
     3181 ggcggcatcg cgcagctccg cgacggtctc gggttgcatg tcgcagccgg tgcgcgaagg
     3241 cacgttgtac atcacgaccg gaatgtcgca acgggcggcg acctcgccga aatggcgccg
     3301 caggccatcc tgggtcgggc gcacgtaaaa cggtgtcacc accagcgcgg cgtcggcgcc
     3361 cagcgacctg gcgcggcggg tcagcgcaat ggtcttcgcg gtggccgcct cgcccgtgcc
     3421 cgcgatcacc ggcacccggt gcgccacgcg ttcgatcgcg aacgccagca gtcgctcgta
     3481 ctcgccctcg tcgagcatgt gcgcttcgcc cgtggagcca gcgactacga tgccctgcgt
     3541 gcccccgtcg agttgatggt cgaccaggcg ggcgaatgca tccaggtcga gcgcaccact
     3601 cgcgtcgaac ggcgtcgcga tggcgcacag gctgccggca atgtccacgg ggtgctccat
     3661 ctcatccaaa cgtcacatca taggacaatc gaatcggcga attgccgccg gccgctgtcg
     3721 tgaaagtccc gggtttttca cctcggcgga aaacgctgcg cgggttcagc tctgcccgtt
     3781 acccgcgcgt cgcggctgca ctccgaggcc gacgccacta tgctggcacg cgtgaccgtc
     3841 aagccagtca ccaccttgaa ccgctccagc ccacgtcccg cccccggtca ccagttgctg
     3901 atccaggccg tcgcaccggc gcgccaatcg ccgctgctgg tgctgtcccg gcggatcgcg
     3961 gacgccggct gcaacctggt cgaatcgcgg gtggcgacgg tcggcgccga cgtgtcggtg
     4021 ctgctgctgg ccaggggcgc ctgggatgcc atcgcgaagc tcgagaccgc gctcgcgcgg
     4081 atcgggcgcg acgagaacct gcacctgctc agctaccgca ccgaagcgcg cgacgagcaa
     4141 agccacctgc tgccatacat ggtcgaggtc gtcgcggcgg acaaggccgg ggtactggtc
     4201 gagttgatcg attt
//
LOCUS       DASSNH010000220         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1455945, whole genome shotgun sequence.
ACCESSION   DASSNH010000220 DASSNH010000000
VERSION     DASSNH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455945"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFK00_07930"
     CDS             complement(<1..230)
                     /locus_tag="VFK00_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HET7592914.1"
                     /translation="MTSSAAATHYPHLFAPLDLGFCTLPNRVLMGSMHTGLEDRARDF
                     GKLAEYFAARARGGVGLMVTGGFSPNIEGWLKP"
     gene            329..2308
                     /locus_tag="VFK00_07935"
     CDS             329..2308
                     /locus_tag="VFK00_07935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7592915.1"
                     /translation="MIRAHRWWLLAVVVLAGLPGLAAAASPFQLEDLRKLVRLSDPQL
                     SPDGKTVAMVATTPNWKTDKHDTAIEVVDVASGTSRAWTRGREDVSSPRWSPDGTRLA
                     FIAKDPDTKQDQIYVMPLAGGDARRITQGKNDVVEFTWSPDSGRLAFITQDPPVNEKD
                     IKAHNKVFQVTDGNFLLRKAVAPWHLWVVAAEGGKPVRLTDGDYSLQTEQEGATPLAW
                     SRDGHSIAFTKFPGAYWGPSFQSVIASVPAQGHGKPATLVDEHGASAFAYAPAGNAFA
                     FLRPRNGDQNNGNAVYVGGAGATRDATAALSRNINAYAWLPDGESLLLQGELGTRSVL
                     WEQPLHGAARVLDLGDVLPGNDLSVARNGEIAFIGSTASHPDELYVMASAHAKPRRLT
                     RINAFVDDLALGRSEAIDWTGPGGFHEDGVLTYPVGYHAGRKYPLVLVIHGGPEGAST
                     VRFSPLPQLLAAAGFLVFQPNYRGSINLGDAYQHAIFRDTGDGPGKDVMAGLAAVEKL
                     GVVDTRRVGVSGWSYGGYMTTWLTGHYDVWKAAVSGAALTDWVMDYTIAYYQMGDLYF
                     FGGSPWSPEYRDIWRSQSPIMYAHQVKAPTLIMGDVGDPNVPLVNSYEWYHALRDNGV
                     TVEFWAYPADTHFPGDIVQQTDVYRRWVDWMKKYL"
     gene            2372..3223
                     /locus_tag="VFK00_07940"
     CDS             2372..3223
                     /locus_tag="VFK00_07940"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HET7592916.1"
                     /translation="MGKFARTWRLMGASWQLLVQDKRLVVFPLVSGIALAAVIGLFAV
                     PMFVAMGGPVHAAAQWHASPQMYLAMFVFYFLAYFVIIFFNAGLIACVLKQMDGEQPT
                     IGYGLAFAWQRLPQICGWALLASSVGMLLRMLEERVGFIGKLIVGLLGMAWSVTSFLV
                     VPVLVVEGSGPIESYRRSVEMLKRTWGEQIIGNVSFGLIFALFGILPAILLFTVAAKF
                     GPAALLAAGAVLVVWLIALGLVQSTLQTIYQAAIYRYAANGEAPPGFDGGMIAAAFRE
                     KPANKWL"
     gene            3231..3770
                     /locus_tag="VFK00_07945"
     CDS             3231..3770
                     /locus_tag="VFK00_07945"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET7592917.1"
                     /translation="MTPGEILRFWFEEATPEQHFKRDDVFDAAIREHFADTYAAAARG
                     ELAAWRTGADGRLAEVIVLDQFPRNLFRGDPRAFACDVVALVLAQEAIHAGADRELPA
                     ARRAFLYMPFMHSESRVIHVDAERLFRQSGLERNYEFELKHKAIIDRFGRYPHRNAIL
                     GRRSTPEELAFLEQPDSSF"
BASE COUNT          656 a         1339 c         1416 g          684 t
ORIGIN      
        1 ggtttcagcc aaccctcgat gttcggcgag aaaccgcccg tcaccatcaa gccgacgccg
       61 ccgcgggcgc gcgcggcgaa gtactcggca agtttcccga aatcgcgcgc gcgatcttcg
      121 aggcccgtgt gcatcgatcc catcagcacg cggttcggca gtgtgcagaa gccgaggtcg
      181 agcggcgcga acaggtgtgg gtagtgggtg gcggcggcgg acgaggtcat tccggcgagc
      241 atgccgccgc agccgggccg cggcaagccg ggcatccgtt gcgctatgct tgccgcgcgc
      301 ccgcatggcg caagctcggg ggactgccgt gattcgcgcg cacaggtggt ggttgcttgc
      361 ggtggtggtg ttggccgggt tgccggggct ggccgcggcg gcgtcgccgt tccagctcga
      421 ggacctgcgg aagctggtgc ggctttccga tccgcagctc tcgccggacg gcaagacggt
      481 ggcgatggtc gcaaccacgc cgaactggaa aaccgacaag cacgacaccg cgatcgaggt
      541 cgtggatgtc gcgagcggca cgagtcgcgc ctggacccgc ggtcgcgaag acgtgtcgtc
      601 gccgcgctgg tcgcccgatg gcacgcgcct cgcgttcatc gccaaggacc cggacaccaa
      661 gcaggaccag atctacgtga tgccgctcgc cggtggcgac gcgcgccgga tcacccaagg
      721 caagaacgac gtggtcgagt tcacctggag cccggacagc gggcggctcg ccttcataac
      781 gcaggatccg ccggtcaacg agaaggacat caaggcgcac aacaaggtgt tccaggtgac
      841 cgacggcaac ttcctgctgc gcaaggccgt cgcgccctgg cacctgtggg tggtcgccgc
      901 cgagggcggg aaaccggtcc ggttgaccga tggcgactat tcgctgcaga ccgagcagga
      961 aggcgcgaca ccgctggcgt ggagccgcga cggccactcg atcgcgttca cgaagttccc
     1021 cggtgcgtac tggggtccgt cgttccaatc ggtgatcgcc tcggtgccgg cgcaaggcca
     1081 cggcaagccc gcaacactgg tggacgaaca cggcgccagc gccttcgcct atgcgcccgc
     1141 cggcaacgcc ttcgcgttcc tgcgcccgcg caacggcgac cagaacaacg gcaacgcggt
     1201 gtacgtcggc ggcgccggcg cgacgcgcga cgcaacggct gcactctcgc gcaacatcaa
     1261 tgcctacgca tggcttccgg atggcgagtc gctgctgctg cagggcgaac tcggcacccg
     1321 ctcggtgctg tgggagcagc cgctgcacgg cgcggcgcgc gtgctcgatc tcggcgacgt
     1381 gctgccgggc aacgacttga gcgtcgccag gaatggcgaa atcgccttca tcggcagcac
     1441 ggcttcccat ccggacgagc tgtacgtgat ggcgtcggcg cacgccaagc cgcggcggct
     1501 gacgcgcatc aacgcgttcg tggacgacct tgcgctcggc cgcagcgaag cgatcgactg
     1561 gaccggaccg ggcggcttcc acgaagacgg cgtgctgacg tacccggtcg gctaccacgc
     1621 cggccgcaag tatccgctgg tgctggtgat ccacgggggc cccgagggtg cctccaccgt
     1681 gcgcttctcg ccgctcccgc aactgctggc ggcggccgga ttcctggtgt tccagccgaa
     1741 ctatcgtggc agcatcaacc tgggtgacgc gtaccagcac gcgattttcc gcgacaccgg
     1801 cgacggcccc ggcaaggacg tgatggcggg actcgcggcg gtcgagaagc tcggcgtcgt
     1861 ggatacccgt cgtgtcggcg tgtcgggatg gtcgtacggc ggctacatga ccacctggct
     1921 gaccggccac tacgacgtgt ggaaggccgc ggtgtcgggt gcggcgctga ccgactgggt
     1981 gatggactac acgatcgcct actaccagat gggcgacctg tacttcttcg gcggctcgcc
     2041 atggtccccc gagtaccgcg acatctggcg gagccagtcg ccgatcatgt acgcccacca
     2101 ggtcaaggcg ccgacgctga tcatgggcga cgtcggcgat cccaacgtgc cgctggtgaa
     2161 cagttacgag tggtaccacg cgttgcgcga caacggcgtc acggtcgagt tctgggcgta
     2221 tccggccgac acgcatttcc cgggcgacat cgtgcagcag accgacgtgt accggcgctg
     2281 ggtcgactgg atgaagaagt acctgtgagc ggccccgtcg gcggggcagg cggtcgtacg
     2341 aaacattccg tgtagcgggg gaggtggcga gatgggcaag ttcgcgcgga cctggcggtt
     2401 gatgggcgct tcgtggcaac tgctggtgca ggacaagcgg ctggtggtgt ttccgctggt
     2461 ttccggcatt gcgctggcgg cggtgatcgg gttgttcgcg gttccgatgt tcgtcgcgat
     2521 gggcgggccc gtccatgcgg ccgcgcagtg gcacgcgtcg ccacagatgt acctggcgat
     2581 gttcgtgttc tatttcctcg cgtatttcgt gatcatcttc ttcaacgcgg gcctgatcgc
     2641 gtgcgtattg aaacagatgg atggcgagca gccgacgatc ggctacggcc tcgccttcgc
     2701 ctggcagcgg ctgccgcaga tctgcggctg ggcgttgctc gcgagcagcg tcggcatgct
     2761 gttgcggatg ctcgaggaac gggtcgggtt cataggcaag ctgatcgtcg gtctgctcgg
     2821 gatggcctgg tcggtgacct cgttcctggt ggtgccggtg ctggtggtcg aaggcagcgg
     2881 cccaatcgag tcgtaccgcc gctcggtgga gatgctcaag cggacctggg gcgagcagat
     2941 catcggcaac gtgagtttcg gcctgatctt cgcgctgttc ggaatcttgc cggcgatcct
     3001 gctgttcacc gtggcggcga agttcggccc ggcggcgctg ctcgcggcgg gggcggtcct
     3061 cgtggtctgg ctcatcgcgc tcgggctggt gcagtcgacc ttgcaaacga tctaccaggc
     3121 cgccatctac cgttacgccg ccaacggcga agcgccgccc ggcttcgacg gcggcatgat
     3181 cgccgcagca ttccgcgaaa agccggccaa caagtggttg tgaggaacgg atgacgcccg
     3241 gggaaatcct gcgattctgg ttcgaagagg caacaccgga acagcatttc aagcgcgacg
     3301 acgtattcga tgccgcgata cgcgaacact tcgcggacac ctacgcggcg gccgcgcgcg
     3361 gcgagctcgc agcctggcgg acgggcgccg acgggcggct cgccgaggtc atcgtgctgg
     3421 accagtttcc gcggaacctg tttcgcggcg atccgcgcgc cttcgcctgt gacgtcgtgg
     3481 cgctggtgct cgcgcaggag gcgatccacg ccggcgccga tcgggagctg ccggcggcac
     3541 gccgcgcctt cttgtacatg cctttcatgc attccgaatc gcgggtgatc cacgtggatg
     3601 ccgagcggct gttccggcaa tctggcctcg agcgcaacta cgagttcgaa ctgaaacaca
     3661 aggcgatcat cgatcgcttc ggccgctatc cgcaccgcaa cgcgatcctc gggcgccggt
     3721 ccacccccga ggaactcgcg ttcctggagc agccggactc gtcgttctga agtctcccac
     3781 ctcgcgaggc aagcgccggg agcaatggac acgtcccacg cggacaagca ccgcaaagat
     3841 cattgttccg cgtttatccg cgtcaaaagc tctttttgcc acccgtttgg cacgtccgca
     3901 atattcttcc gcagcgaagc gctaccccgg cttggccgcg cccgcgcggc gccgcatcca
     3961 ccagccgacc ccggccaccc ccgccaccag caccgcgtcc accaggtaac gcagcggatc
     4021 gtgggcgtcg aaccacccct cgagcaggtc gtcgtgggtg atcatccggg ccgaggtcca
     4081 ggcgatcgcg gccgc
//
LOCUS       DASSNH010000221         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_515189, whole genome shotgun sequence.
ACCESSION   DASSNH010000221 DASSNH010000000
VERSION     DASSNH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515189"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFK00_07950"
     CDS             <1..259
                     /locus_tag="VFK00_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592918.1"
                     /translation="YFVQSVVGPGIYTFSATAGGAAIALTNAGTGTSFLGAVPEGIKS
                     WMKLRLGTFDVARDEPSLEPVIGGVQAFTDRLLDEFTTFEF"
     gene            263..613
                     /locus_tag="VFK00_07955"
     CDS             263..613
                     /locus_tag="VFK00_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HET7592919.1"
                     /translation="MSILDIGSLNRQITIQAKQSGVDAAGQPIDTWTNVATVWANIKG
                     ETGMASIRKTLPRDGVAMSLDAYSFRIRFLAGIVAGMRVLYDGATFDILQVRMDYAGR
                     VWTDLVCEAGRYDD"
     gene            767..1279
                     /locus_tag="VFK00_07960"
     CDS             767..1279
                     /locus_tag="VFK00_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HET7592920.1"
                     /translation="MAGTELFDDRAVQASLSKLGGQLSVSLARSMGVAGGKIMRDTAK
                     ALAPEYDGSTGLAGGKNVDIPPVPGLLKSAIYLAFKERLSDSGHVTYGISWNSKIAPH
                     GHLLEFGHWRLNKIVNGIPTNIPLKTPKWVPAHPFLRPAYNVALVPARAAMFERGRER
                     LPELLREAKA"
     gene            1276..1629
                     /locus_tag="VFK00_07965"
     CDS             1276..1629
                     /locus_tag="VFK00_07965"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HET7592921.1"
                     /translation="MSLEASLFTILSPLVSGNVYPDITPEPPVFPCIIYQQVGGKALD
                     YADKTLPANDNARVQVLVWAKTRLEASTLIRQVRVALMASTLNVRTVAAAISQYHEYL
                     ALYGARQDFDIDYPP"
     gene            1704..2360
                     /locus_tag="VFK00_07970"
     CDS             1704..2360
                     /locus_tag="VFK00_07970"
                     /inference="COORDINATES: protein motif:HMM:NF020392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET7592922.1"
                     /translation="MQSFPNGTTFSVSTAFSAAVPFTAVTNAAPPVCSAAAPPTDGTV
                     GLITSGWAALNERVAKTTGMVASTSFELEGIDTTDTTKYPAGQGAGDFTPVSTWVQLS
                     QVTNVQKSGGTQQFYKWQYAEDPSSTQKQRPTFKDAKTLTLTLDYDPALAWYDALSKA
                     DAAQSIVILKAVLPSGDTLYYAVYPSFDADPSMDLNKNMENTATFSLVSPLTRYAAGA
                     "
     gene            2360..2668
                     /locus_tag="VFK00_07975"
     CDS             2360..2668
                     /locus_tag="VFK00_07975"
                     /inference="COORDINATES: protein motif:HMM:NF020330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HET7592923.1"
                     /translation="MFSIEPNPTFKASLVIVGQGREQTLNVVYRHKPRSEYIALLEKV
                     RSGDVPVADALLQLVESWDADAPLEAGTIEKLQEAQPGVDWAIVSGYTEALTVARKGN
                     "
     gene            2665..2976
                     /locus_tag="VFK00_07980"
     CDS             2665..2976
                     /locus_tag="VFK00_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1799 domain-containing protein"
                     /protein_id="HET7592924.1"
                     /translation="MTAAVQALYWRRPTTAELGDFGVTPDDFPEPHVDLWPENWPPIQ
                     LFIHHCTQWRVGAAGPVGLDYGVIFHELDRTGVTGDDFEDMMGCIRIIETAALEAINE
                     K"
     gene            2980..>3176
                     /locus_tag="VFK00_07985"
     CDS             2980..>3176
                     /locus_tag="VFK00_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592925.1"
                     /translation="MAVEGENLGTAAIYLTVDTTSMDAGIAKAKTKLAGMSAEAEKQY
                     TAMTASQRKVADSLVKQAELI"
BASE COUNT          602 a         1026 c          985 g          563 t
ORIGIN      
        1 ttatttcgtg cagtcggtgg tcggacccgg catctacaca ttttccgcga ctgctggggg
       61 cgccgcgatc gcgctcacca acgccggcac cggcaccagc ttcctgggcg cggtcccgga
      121 gggcatcaag tcgtggatga agctgcgtct tggtaccttc gacgtcgcgc gcgatgaacc
      181 atcgctggaa cccgtgatcg gtggcgtgca ggccttcacc gaccggctgc tggacgagtt
      241 cacgaccttc gagttctgac gcatgtccat cctcgacatc ggctcgctga accgccagat
      301 cacgatccag gccaagcaat ccggcgtgga cgcggcgggc cagccgatcg acacctggac
      361 gaacgtagcc acggtctggg ccaacatcaa gggcgagacc ggcatggcct cgatccggaa
      421 gacgctgccg cgcgacggcg tcgcgatgtc gctggatgcc tactcgttcc gcatccgctt
      481 cctggccggc atcgtcgccg gaatgcgcgt gctgtacgac ggtgcgacct tcgacatcct
      541 gcaggtgcgc atggattacg cggggcgcgt ctggactgat ctcgtttgtg aggcaggtcg
      601 ctatgacgat tgagcatgca acgatcacgc ttcgtttccg tcagcgcgag tggtcgaagc
      661 ggttcgtaaa gtggtgcatg cgccatcggc gtaccgcacg catcttggga attccggcga
      721 tcgtttggat tgcttcgctc ggcttcattg tcgaggtcga gaactgatgg ctggtacgga
      781 actgttcgac gatcgcgcgg tgcaggcctc gctgtcgaag ctgggcggcc agctttcggt
      841 gagcctggcg cgctcgatgg gcgtcgccgg cggcaagatc atgcgcgaca ccgcgaaggc
      901 gctcgcgccg gaatacgacg gttctaccgg gcttgcgggc ggcaagaacg tggacattcc
      961 gccggtgccg ggcctgttga agtcggcgat ctacctggca ttcaaggagc ggctgtccga
     1021 cagcggccac gtcacctacg gcatttcgtg gaacagcaag attgcgccgc acggccactt
     1081 gctggaattc ggccactggc gcctgaacaa gatcgtgaac ggcattccga ccaacattcc
     1141 gctgaaaacg ccgaagtggg tgccggcgca cccgttcctg cgacccgcct acaacgtggc
     1201 actcgtgcct gcccgggccg cgatgttcga acgcggccgt gaacggctgc cggagctctt
     1261 gcgcgaggcg aaagcgtgag cctcgaagcc agcctgttca cgatcctttc gccgctggtg
     1321 agcggaaacg tgtatccgga catcacgccg gagccgccgg tgtttccctg catcatctac
     1381 cagcaggtgg gcggcaaggc gctcgactat gcggacaaga cgctccccgc gaacgacaac
     1441 gcgcgcgtgc aggtccttgt atgggccaag acacggctgg aagcctcgac gctcatccgg
     1501 caggtccgcg tggcgctcat ggccagcacg ttgaacgtgc gtaccgtggc cgccgccatt
     1561 tcccaatacc acgaatacct cgcgctgtat ggcgcgaggc aggacttcga catcgattat
     1621 ccaccctgat tcaccgtttc acctgttgat cccaccaccg gcctcgtgcc ggtttttttg
     1681 ttcaacccgc cgaggacacg aagatgcaga gctttcccaa tggcaccacc ttcagcgtat
     1741 cgaccgcctt ttccgcggcc gttcccttca ccgccgtcac caacgccgcg ccgcccgtgt
     1801 gcagcgccgc tgcgccgcct accgatggca ccgtgggcct gatcacttcg ggctgggccg
     1861 ctctgaacga gcgcgtcgcc aagaccaccg gcatggttgc cagcacttcg ttcgagctcg
     1921 aaggcatcga taccaccgat accacgaagt acccggccgg gcagggcgct ggcgacttca
     1981 cccctgtttc gacgtgggtg cagttgtcgc aggtaaccaa cgtgcagaag tctggcggca
     2041 cccagcagtt ctacaagtgg caatacgccg aagacccgtc cagcacccag aagcaacgcc
     2101 cgaccttcaa ggatgcgaaa acgctgaccc tgacgctgga ctacgacccg gcgctcgcgt
     2161 ggtatgacgc gctctccaag gccgacgcgg cgcagtcgat cgtgatcctg aaggcggtgc
     2221 tgccatccgg cgacaccctg tactacgcgg tatacccgtc attcgacgcg gatccgtcga
     2281 tggatctcaa caagaacatg gaaaacacag ccacgttctc gctggtgtcg ccgttgaccc
     2341 gctacgcggc aggtgcgtga tgttctcgat cgaacccaac ccgacgttca aggcgtccct
     2401 ggtcatcgtt ggccagggac gcgagcagac gctcaacgtc gtctatcgcc acaagccgcg
     2461 ctcggaatac atcgcactgc tggaaaaggt gcgaagcggc gacgtgccag tcgcggatgc
     2521 gttgctgcag ctggtcgaat catgggacgc ggacgcgcca ctcgaagccg gcaccatcga
     2581 aaaactgcag gaagcacaac ccggcgtcga ctgggccatc gtgtccggtt acaccgaagc
     2641 cttgacggtg gcccgcaagg gaaattgacg gccgccgtgc aggcgctgta ttggcggcgc
     2701 ccgaccacgg cggagcttgg cgatttcggc gtgacgccag atgactttcc cgagccgcac
     2761 gtcgatctct ggcccgagaa ctggcccccg atccagttgt tcatccatca ctgcacgcag
     2821 tggcgcgtgg gcgcggcggg tccggttggg ctggattacg gcgtgatctt ccacgagctc
     2881 gaccgcaccg gcgtaaccgg cgacgacttc gaggacatga tgggctgcat ccgcatcatc
     2941 gaaaccgccg cacttgaagc gatcaacgag aaatgaccca tggccgttga aggcgaaaac
     3001 cttggcactg ccgcaatcta cctgacggta gacacgacca gcatggatgc tggcattgcc
     3061 aaggccaaaa ccaagctggc gggcatgtcg gcggaagcgg agaagcagta caccgccatg
     3121 accgcgagcc agcgcaaggt cgcggacagc ctcgtcaagc aggccgagtt gatcaa
//
LOCUS       DASSNH010000222        12659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1269505, whole genome shotgun sequence.
ACCESSION   DASSNH010000222 DASSNH010000000
VERSION     DASSNH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269505"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFK00_07990"
     CDS             <1..360
                     /locus_tag="VFK00_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET7592926.1"
                     /translation="QLAAANKRVANILRKEGIDAEAAARHELDAELLCEPAGQALAQA
                     LDEARSDNAPRLDVAAADRDYAGALARLARLQAPIDRFFDEVMVMAEDPKLRAARVSL
                     LARIKARFDAIADIALL"
     gene            360..1955
                     /locus_tag="VFK00_07995"
     CDS             360..1955
                     /locus_tag="VFK00_07995"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592927.1"
                     /translation="MASTAQPAGPSSNPRWLEGRQHLLRGQLDAAHHALASMRIHEPA
                     AALTHLLAAQIAWRENRIREGAAFALEAARAGSTDPETLCTVVAVLLVTGEIVAAREL
                     LEHPALAACEDPLLLMRIAGHRKRLEEHTEALALLDRAQAQGHGSPALEFRRGEELMF
                     NGRLPEAEASFAESLARAPGHGHVAVPLVRLRTQTPDHNHLELIARSLDAVKPGGTDH
                     AALEFARYKTLEDLGRDQEAWEALAHGNALMYARLRDQAEQRSAGLDRFLEQCVPGIR
                     RASARPVDGARPIFVVGMPRSGSTLLERMLGNHPEVRVTGELPDMGSQLHWVADSSNA
                     HPDVLLARLPGVDFAEVGRRYLAQTQWRAQGKRFYVDKHSPNWSLAGVIHAALPQAPI
                     LNLVRDPMDVCFSNWRMFFGDASAWSYDQPTLARYCADYRRTMARWHALMPGAILDVH
                     YADLVHEPEATLRKVLEFCGLEWQPGCADISRNPAPSSTLSTAQVRGRVHTRAFGEWR
                     RYEPQLSVLARVLAQGPDPVGSP"
     gene            1952..3508
                     /locus_tag="VFK00_08000"
     CDS             1952..3508
                     /locus_tag="VFK00_08000"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592928.1"
                     /translation="MSRSVERRWERAAQYLAQGQVAAARVQLEALEAAAPSDYRTSLL
                     AADLAWREDRIRDAVRLALAANEAAPDESQALCGVVESLLRVGEVVAARECLARPALA
                     RATEPALLLRLSDFRQRLDENVESLALIERAIAAGADGAEVGFHHGVQLYFNGRLADA
                     ESELEACVRQAPAAGRAALALSRLRKQTRDSNHLDSLAAGLARVAPGTRDHAALEFAH
                     SKELDDLGRFGEAWDALARGNAIMRTRNPFDPAAQHTYLERLIDACDQQRVRTDEAVN
                     AEGPQPIFIVGMARSGTTVLERMLGNHSGVASAGELVDFGHQLHWAADTSNTQGQAFL
                     ARLPDLDLAEVGRRYLAQTQWRARGKSFYIDKQPPNWVLAGLIHAALPHARLLHMVRD
                     PMDLCFSNWRAFFGETYAYSYDMAHLAAYHQDYLRMMAHWHRVMPGTILDVAYADLVS
                     DPESAMRRVFAHCGLPWEPGCLDIRSNAAPVATLSAAQVRAGLHGGAFGEWRRYQSQL
                     EPLRRALGAA"
     gene            3505..5100
                     /locus_tag="VFK00_08005"
     CDS             3505..5100
                     /locus_tag="VFK00_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592929.1"
                     /translation="MSSHPGGPRFERQWQRAQRYIASRQFAAARAALEALLVHRPDDI
                     QARLLLASVLLEQEHLRDACDELVQASRYLPDDPAIVHRVAYCLHQVGESVGVHQCLQ
                     HPAIEHARDGGVLVALAHLHQLQGTHEQALALMDRALQLGFDSPDVRYFRSLQLQFNG
                     RMREAEAELEACLRIGPTYGRASLALARMRRQTPEHNHLEAITSRLERVEPGSEHHAA
                     LEFARFKELDDLDLRDEAWAALVRGNNIMRARVPHDSERERRRFDALITTCDEAFVRA
                     TEDDVAAAGVDEGPQPIFIVGMPRSGTTLLERILGNHSQVASPGELPDFPRQLRWVAD
                     THGADLLDEKLLERLPDLDYRLLGQRYLAQSRWRANGKRFYVDKLPPNFMLAGLIHRA
                     LPHAPILHMRRDAMDVCYSNWKALFGESYAYSYGMDTLAAHFREYARLMAHWRGVMPG
                     AILDLDYEQVVTDPEAATAEVLSFCGLPFEAGCYDITRNRNPVSTLSSPQVRETIHRR
                     ALGEWRRYASHLEPLRKALAAGD"
     gene            complement(5199..8066)
                     /locus_tag="VFK00_08010"
     CDS             complement(5199..8066)
                     /locus_tag="VFK00_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017163068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592930.1"
                     /translation="MNRKLLASAICASLFVTGTAFAQGAPAPQQTQDQSPTNAASSQD
                     QDQNQDKTKTLQAITVTGSLIPQSQIETANPVLTISSQDMIRKGYSNVYEALRAQPLS
                     TGAVQDSQFSQGFTPGAQTISLLGLSPDFTLFLIDGRPMADYPLLYNGSANFTDLSNI
                     PMSMVDHIDILPGNQSSIYGSSAIAGVVNIILKKRMEGYNLNYRAGGYSQGGGQNMRL
                     ALSGGYNTDKLHLMYALQLDDQHPIFGFNRDLTSSTQNAPNPDSRNPFTVGVHWYFDA
                     GNHYLNPNTDAPGVGCSAMSGLFNGTTQLSYRPNRDDGHYCGSYIAPGYATLLNKKKD
                     GSGYLNFSYDLSPNAELYGHMLYSVQKQNWYAGPNYNWWAPDNGNYIINNNTGRFEFY
                     ERIFAPEEIGGLLVNSNYELSRMYSGNIGIRGNFGQSDWQYDAYVARSDYKSYDKWLH
                     PLTDEIDSFFRDKFLGPLLGTYYGYSIYAPDDAAFWQPLTPAEYQGFSDYVRATSHTY
                     TQNANLQITNSDLFELPAGAVGFAGVLQVGNQYWDNPIDPRLGAGDFWGYTGTSGNGK
                     RDNYAAAVELRVPIFSMLTADLSGRYDHYSNQEASSDSKATYKLGLEFRPFDTLLLRA
                     NYATAFRAPDMAYIFQGPSGFYSSTTDYYKCEKYEASTPLPDCTWYSFQYLGSQQGNP
                     DLKSITAKSWGYGLVWSPTTSLTLKADYYNVKIDNEVSPLSVSGLLQNEADCRLGTLD
                     PGSPTCIDALNRVQRTAPNANPQFSENITQIIVGPINVSHERVSGIIASGDWRVDAGR
                     FGDFTFGARYNVTMDHTYQQYPGDPDHDLLHEPRWSSEFKTIANASVTWDVGKWSTTI
                     QGQRYGATPNYAAQVYGWEGGGDQYSQAGTVGPWFVYNGSVTYNVTPDITTKLIVQNI
                     FDKMPPKDQTWVGWPYYNQFNYTPWGRAIWLDLNIHFGSGS"
     gene            8580..10202
                     /locus_tag="VFK00_08015"
     CDS             8580..10202
                     /locus_tag="VFK00_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592931.1"
                     /translation="MAANPATRRFDADLLWQKAQGSLVGGQLQEARQVLTAYKALGRG
                     DDVHAHLLAGQIALIEDRIRDGARNVLDAARLAPDDPHALAAVAGMLLQLGENVAALE
                     CLDRPSLAGCEDPLLLMQLAAHRRRLQQHAEGLALLDKAQSLGCDDPALRYMRGEALL
                     HSGRLEEAEAELASCLADAPERGRVAVPLVGLRRQTPERNYLAALDAGTRMAPSGTSD
                     QAAFEFARYKTLEDLGRNDEAWQALVHGNKLMHARLPERLRRPQPLSDRFLEAWPAAS
                     GDPAPAGESGPQPIFIIGMPRSGTTVLERMLGNHSEVTAAGELMDFALQMHWTADTRN
                     LAGDQFLSRLPGLDYGELGRRYLAQTRWHGRGKAFFIDKQPPNWMWAAAIRHALPRAP
                     ILNLVRDPMDVCFSHWRTYFGESCAYSHDFQTLAGVYRDYRRAMAHWHRIMPGAILDV
                     PYEELVRDPDATLRKVFDFCGLAWEPGCADITRNAAPSATPSAAQVRSSLHTRGFGEW
                     RRYESQLQSLADAIAAGGEFGSGNEDRPASGS"
     gene            10435..11790
                     /locus_tag="VFK00_08020"
     CDS             10435..11790
                     /locus_tag="VFK00_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7592932.1"
                     /translation="MLLGELIGGLLKVGEMVAGRRLLDDPAISTSADPNVLVRAARQR
                     KALGEHKAALALIERAARAGARGPTFLFQRATQLLFNGRIEEAEADLEQCIASGSPPT
                     RAYLELATLRTQTAQRNHLRAIKLAMDRVDPKTEDYAFLEFAYYKELEDLGRFGDAWR
                     ALERGNAVLHALMPYDAEHESAMFDRLMEVCTPEFLRSETDDSDEGPQPIFVVGLPRS
                     GTTVLERILGNHSQIESAGELVAFPRALAWMVDHDSPGVMDDVTVERLVDADWPAIGQ
                     RYLEQTRWHARGNAFYVDKLPPNWILAGLIHKALPHARIINLMREGVGVCFSNWRACL
                     GTHPEYRYAYDLGSLVAHFRQHRRLMAHWHRVLPGKILDVDYASLVRDPEATAREIFA
                     FCGLPFEPHCTDIAGNLSPVSSLSAPQVREPIHTRSSASWKPYAAQLEPLIRALSSPT
                     G"
     gene            complement(12166..>12659)
                     /locus_tag="VFK00_08025"
     CDS             complement(12166..>12659)
                     /locus_tag="VFK00_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7592933.1"
                     /translation="FGLFTFNFQGTYYDLQNQKPDNLPDTPLNHSNSFGSAFRYRVNA
                     SLDWSLGNWGATWTTRLYSAIKEQCSYDNTAEGGPECNTPGHYENGVVSNLNITGDTV
                     FNDLQVRYTIAPIQATVSLGVNNVFDRFGPILYSAPSSSIAPYYGGFDIGRFYYVRYN
                     QKF"
BASE COUNT         2095 a         4117 c         4265 g         2182 t
ORIGIN      
        1 cagctcgccg ccgccaacaa gcgcgtcgcc aacatcctgc gcaaggaagg catcgacgcg
       61 gaagccgcgg cacggcatga actggacgcg gagctgttgt gcgaaccggc cgggcaggcc
      121 ttggcgcagg cgctggatga agcgcgcagc gacaacgcgc cccggctgga tgtcgccgcc
      181 gccgaccgcg attacgccgg cgcgctggcg cgcctcgccc ggttgcaggc gccgatcgac
      241 cgcttcttcg acgaggtcat ggtgatggcc gaggatccga agttgcgcgc cgcccgcgtc
      301 tcgctgctgg caaggatcaa ggcccgcttc gacgcgatcg ccgacatcgc actgctgtga
      361 tggcatcaac ggcccagccc gccggcccgt cctccaatcc gcgctggctc gaagggcgcc
      421 agcacctgct gcgtggccag ctcgatgctg cgcaccacgc attggcatcg atgcgcatcc
      481 acgaaccggc tgcggcgttg acgcacctgc tcgccgccca gatcgcctgg cgcgaaaacc
      541 ggattcgcga gggcgcggcg ttcgccctcg aggcggcgcg cgcagggtcc acggatccgg
      601 agaccctctg caccgtggtc gccgtgctgc tggtcaccgg cgagatcgtc gcggcgcggg
      661 aattgctgga gcacccggcc ctcgcggcgt gcgaagaccc cctgctgctg atgcggattg
      721 ccggccatcg caaacgcctg gaggagcaca ccgaagcgct ggccctgctc gatcgcgcgc
      781 aggcgcaagg ccacggctcg cccgcactcg agttccggcg cggcgaggaa ctgatgttca
      841 acgggcgcct cccagaggcc gaagcttcgt ttgcggaatc gctggcgcgg gcgcccggcc
      901 atggacacgt cgcggtgccg ctggtgcgcc tgcggaccca gacgcccgac cacaatcacc
      961 tggagttgat cgcgcgcagc ctggatgcgg tcaagccggg cgggacggac cacgcggcgc
     1021 tcgaattcgc gcgctacaag acgctggagg atctcgggcg cgaccaggaa gcgtgggagg
     1081 cgcttgccca tggcaatgcc ctgatgtacg cgcgcttgcg ggaccaggcc gaacagcgaa
     1141 gcgcgggtct cgatcggttc ctcgagcaat gcgtgcccgg catccggcgc gcgagcgcac
     1201 gcccggtgga cggtgcgcgg cccatcttcg tcgtgggaat gccacgctcc ggctcgacgc
     1261 tgctcgaacg catgctcggc aaccatcccg aagtcagggt caccggcgag ttgcccgaca
     1321 tggggtcgca gttgcactgg gtcgccgact cgagcaacgc ccatcccgat gtcctgctgg
     1381 cgaggctgcc gggcgtcgac ttcgcggaag tcgggcggcg ctacctcgcg cagacgcagt
     1441 ggcgggcgca gggcaagcgg ttctacgtcg acaaacactc gcccaactgg tcgcttgcag
     1501 gcgtgatcca tgcggcgttg ccgcaggcgc cgatcctcaa cctggtccgc gaccccatgg
     1561 atgtgtgctt ctccaattgg cgcatgttct tcggcgatgc cagcgcctgg agctacgacc
     1621 agccgacgct ggcccgctac tgcgccgatt accgccgcac gatggcgcgg tggcacgcat
     1681 tgatgccggg cgccatcctg gatgtccact acgcggacct ggtgcacgaa cccgaagcga
     1741 ccctgcgcaa ggtcctcgag ttctgcggac tggaatggca acccggatgc gccgacattt
     1801 cgcgcaaccc ggcgccgagc tccacgttga gcacggcgca ggtgcgtggg cgcgtgcaca
     1861 cccgcgcatt cggagaatgg cggcgctatg agccgcaact gtccgtgctg gcgcgggtgc
     1921 tggcgcaggg gccggatccg gtggggagcc catgagccga tccgtcgagc ggcgctggga
     1981 gcgggccgcg cagtacctcg cgcagggcca ggtcgcggcc gcccgcgtgc aactggaggc
     2041 gttggaggct gcggcgccct cggattaccg gacgagcctg ctggcggcgg acctggcgtg
     2101 gcgcgaggac cggattcgcg acgcggtgcg gcttgcgctc gccgcgaacg aggcagcgcc
     2161 ggacgaatcg caggcactgt gcggcgtggt ggaatcgttg ctgcgggtgg gcgaagtggt
     2221 cgccgctcgc gagtgcctgg cgcggccggc gctcgcccgc gccaccgagc ccgccttgtt
     2281 gttgcgcctc tcggacttcc gccagcgcct ggacgaaaac gtcgaatcgc tggcattgat
     2341 cgagcgggcg atcgcggccg gtgcggacgg cgcggaggtc ggcttccatc acggggtgca
     2401 gctctatttc aacggccgcc tcgcggacgc cgagtcggaa ctcgaggcat gcgttcggca
     2461 ggccccggcc gccggtcgcg ccgcgctcgc cctgtcgagg ctgcgcaagc aaaccaggga
     2521 cagcaaccat ctcgattcac tggctgcggg ccttgcccgc gtcgccccgg gtacgcgcga
     2581 ccacgccgcg ctggaattcg cgcactccaa ggaactggat gacctcggtc gcttcggcga
     2641 agcgtgggac gcgctcgccc gcggcaacgc gatcatgcgg acgcgcaacc ccttcgaccc
     2701 cgctgcgcag cacacctacc tcgaacggct gatcgacgct tgcgaccaac agagggtacg
     2761 gacggacgaa gccgtgaatg ccgaaggacc gcagcccatc ttcatcgtcg ggatggcgcg
     2821 ctccggcacc accgtcctcg agcggatgct cgggaatcat tccggcgtgg ccagcgccgg
     2881 cgagctggtg gacttcggcc accagttgca ctgggcagcc gacacgagca acacgcaagg
     2941 acaggcgttc ctcgcgcggt tgccggatct cgacctcgcg gaagtcgggc gccgttacct
     3001 cgcgcagacg cagtggcgcg cgcggggcaa gtccttctac atcgacaagc agccgccgaa
     3061 ctgggtgctg gcgggcctga tccacgcggc gttgccccac gcgcggctgc tgcacatggt
     3121 gcgcgatccg atggacctgt gcttttccaa ctggcgcgcg ttcttcggcg agacctatgc
     3181 ctacagctac gacatggccc acctcgccgc ctaccaccag gattacctgc gcatgatggc
     3241 gcactggcac cgggtgatgc ccgggacgat cctggatgtc gcgtatgccg acctcgtgag
     3301 cgaccccgag tccgcgatgc gccgggtgtt cgcccattgc ggactgccct gggagcccgg
     3361 ttgcctggac attcgcagca acgcggcacc ggtggcgaca ttgagcgccg cgcaggtccg
     3421 tgcgggtctc cacggtggcg cattcggcga gtggcgacgc taccagtccc agctcgagcc
     3481 gctgcggcgc gcgctggggg cagcatgagc tcccatccgg gcgggccgcg cttcgagcgc
     3541 cagtggcagc gtgcgcagcg ttacatcgcc tcgcgccaat ttgccgcggc tcgggcggcg
     3601 ctggaggcgc tgctcgtgca ccgcccggac gacatccagg cgcgactgtt gctggccagc
     3661 gtgctgctcg agcaggaaca cctgcgtgac gcctgcgatg aactggtgca ggcgtcccgg
     3721 tacctgccgg acgatccggc catcgtccat cgcgtggcgt attgcctgca ccaggtcggc
     3781 gaatcggtgg gcgtccacca gtgcctgcaa catccggcga tcgagcatgc gcgcgatggc
     3841 ggcgtgctgg ttgcgctggc gcacctgcac caactccagg gcacgcacga gcaagcgctg
     3901 gcactgatgg atcgtgcgct gcaactcggc ttcgacagcc ccgacgtgag gtacttccgg
     3961 agcctgcagt tgcaattcaa cggccggatg cgcgaagcgg aggccgagct cgaggcctgc
     4021 ctccggatcg gccctaccta cgggcgcgcc tcgctggcgc tcgcgcgcat gcgccggcaa
     4081 accccggaac acaatcatct cgaggcgatc acgtcgaggc tcgagcgtgt cgagccgggc
     4141 agcgagcacc acgcggcact cgagttcgcc cgcttcaagg aactcgacga cctggacctg
     4201 cgcgacgagg catgggcggc gctggtgcgc gggaacaaca tcatgcgggc ccgtgttccg
     4261 cacgacagcg agcgcgaacg ccgccgcttt gacgcgctga tcacaacctg cgatgaagcc
     4321 ttcgtccggg caacggaaga tgacgtagcc gcggccgggg tcgacgaggg gccacagccg
     4381 atcttcatcg tgggcatgcc gcgctcgggc acgaccttgc tggaacgcat cctcggcaac
     4441 cattcacagg tcgcgagccc gggcgagctt ccggatttcc ccaggcagtt gcgctgggtc
     4501 gccgacacgc acggcgcgga tctgctggac gaaaagctgc tcgagcgcct cccggacctc
     4561 gattaccggt tgctcgggca gcgctatctc gcccagagcc gctggcgcgc gaacggcaaa
     4621 cgcttctacg tcgacaagct gcctcccaac ttcatgctgg ccggcctgat ccaccgggcg
     4681 ctcccgcacg cgccgatcct gcacatgcgg cgcgacgcca tggacgtgtg ttattccaac
     4741 tggaaggcgc tgttcggcga gtcgtacgca tacagctacg gcatggatac cttggcggcg
     4801 catttccgcg aatacgcgcg cctgatggcg cattggcgcg gggtgatgcc cggagccatc
     4861 ctcgacctcg attatgaaca ggtcgtcacc gacccggaag cggctaccgc cgaagtcctg
     4921 tcgttctgcg ggctgccgtt cgaggctggc tgctacgaca tcacccgcaa ccggaatccg
     4981 gtatcgacgc tgagcagtcc gcaggtccgc gagacgatcc accgccgggc gctcggcgaa
     5041 tggcgccgct acgcgtcgca cctggagcca ctccggaagg ccctcgccgc cggcgactga
     5101 tttccggatg ctttccgggc cgcgcgcccc gcagccacat ggccaaaaaa aggggccggc
     5161 gtttgccggc cccgagtgca ttgcttcggt gcttcgcgtc agcttccgct gccgaagtgg
     5221 atgttgaggt ccagccaaat ggcgcggccc cagggcgtgt agttgaactg gttgtagtac
     5281 ggccagccca cccacgtctg gtccttcggc ggcatcttgt cgaagatgtt ctgcacgatc
     5341 agcttggtcg tgatgtccgg cgtcacgttg taggtgaccg agccgttgta cacgaaccac
     5401 ggcccgaccg tgcccgcctg cgagtactgg tcgccgccac cttcccaacc atagacctgc
     5461 gcggcgtaat tcggggtggc accgtagcgc tggccctgga tcgtggtgct ccacttgccg
     5521 acgtcccagg tcacggacgc attggcgatg gtcttgaact cgctcgacca gcgcggctca
     5581 tgcagcaaat cgtggtccgg atcgcccggg tactgctggt acgtgtggtc catcgtcacg
     5641 ttgtagcgcg cgccgaaggt gaagtcgcca aacctgcccg cgtccacccg ccaatcaccc
     5701 gaggcgatga tgccggacac ccgctcgtga gacacgttga tcggcccgac gatgatctgg
     5761 gtgatgtttt ccgagaactg cggattggca ttgggcgccg tgcgctgcac gcggttgagc
     5821 gcgtcgatgc aagtcggcga gccgggatcg agggtgccga gccggcagtc ggcttcgttc
     5881 tgcagcaggc cgctgacgct gagcgggctg acttcgttgt cgatcttgac gttgtagtag
     5941 tcggccttca gggtcaggct ggtggtcggc gaccacacga ggccgtagcc ccaggacttg
     6001 gcggtgatcg acttcaggtc ggggttgccc tgctgcgagc caagatactg gaagctgtac
     6061 caggtgcagt ccggcaaggg agtgctggcc tcgtacttct cgcatttgta gtagtcggtc
     6121 gtgctgctgt agaacccgct cggcccctgg aagatgtacg ccatgtccgg tgcgcggaac
     6181 gccgtcgcat agttggcgcg cagcaacagc gtgtcgaacg gacggaactc gagccccagc
     6241 ttgtaggtcg ccttgctgtc cgagctggct tcctggttgc tgtaatggtc gtagcggccg
     6301 gagagatccg cggtcagcat gctgaaaatc ggtacgcgca actccaccgc cgcggcatag
     6361 ttgtcgcgct tgccgttgcc ggaggtgccg gtatagcccc agaagtcgcc cgcgcccagt
     6421 cgcggatcga tcgggttgtc ccagtactgg ttgccgacct ggaggacgcc ggcgaagccc
     6481 accgcgcccg cgggaagctc gaacagatcg gagttggtga tctgcaggtt ggcgttctgg
     6541 gtgtacgtgt gggacgtcgc gcgcacgtag tccgagaagc cctggtactc ggccggcgtc
     6601 agcggttgcc agaaagccgc gtcatcgggc gcgtagatgc tgtagccgta gtaggtgccg
     6661 agcaagggac caaggaactt gtccctgaag aaggaatcga tctcgtccgt cagcgggtgc
     6721 aaccacttgt cgtaggactt gtagtcggaa cgcgcgacat acgcatcgta ctgccagtcg
     6781 gactgtccga agttgccgcg gatgccgatg ttgccggagt acatgcggga cagttcgtag
     6841 ttggagttga ccagcaggcc gccgatttcc tccggcgcga agatccgctc atagaattcg
     6901 aaacgaccgg tgttgttgtt gatgatgtag ttgccgttgt ccggcgccca ccagttgtag
     6961 ttgggaccgg cgtaccagtt ctgcttctgg accgaataca gcatgtgtcc gtacagctcg
     7021 gcattggggc tcaagtcgta gctgaagttg aggtaccccg agccgtcctt cttcttgttc
     7081 agcagcgtcg cgtagcccgg cgcgatgtag ctgccgcaat agtgcccgtc gtcgcggttg
     7141 gggcgatagc tgagctgggt ggtgccgttg aacaagccgc tcatcgccga gcagccgaca
     7201 cccggcgcat cggtgttcgg attgaggtag tggttgccgg cgtcgaaata ccagtgcacg
     7261 cccaccgtga atggattgcg gctgtcgggg ttgggcgcgt tctgggtgga ggaggtcagg
     7321 tcgcggttga agccgaaaat cgggtgctgg tcatccagct ggagcgcgta catcaggtgc
     7381 agcttgtcgg tgttgtagcc gcccgacagc gccagccgca tgttctgccc gccaccctgc
     7441 gaatagccgc ccgcccggta gttcaggttg tagccttcca tccgcttctt gaggatgatg
     7501 ttgaccacgc ccgcgatcgc cgacgaaccg tagatcgacg actggttgcc cggcaggatg
     7561 tcgatgtggt cgaccatcga catcggaatg ttggagaggt cggtgaagtt ggccgagccg
     7621 ttgtagagca acgggtagtc cgccatcggg cggccgtcga tcaggaacag ggtgaagtcg
     7681 ggcgacaggc ccagcaggct gatggtttgc gcccccggcg taaacccttg ggagaactgg
     7741 ctgtcctgga ctgcgccggt cgaaagtggc tgggcgcgca gcgcctcgta cacgttcgag
     7801 tagcccttcc tgatcatgtc ctgcgagctg atcgtgagta ccgggttggc ggtctcgatc
     7861 tgcgattgcg gaatgagcga tcctgtgacg gtgatcgcct gcagggtctt ggtcttgtcc
     7921 tggttctgat cctgatcctg cgaagacgcg gcattggtgg gggactgatc ctgcgtctgc
     7981 tgcggcgcag gagccccctg ggcaaaggca gtgcccgtga caaacaggct ggcgcaaatg
     8041 gctgaggcca agagcttacg attcatcaga agtactcccc gggtgatgct gtttcggttg
     8101 tggggcggcg gaatgtccga cccgttcgat agtgaggccg cgccggcccg tgggcccgca
     8161 gcataagtga ttgattccct gcgaattcgc gtcaaaaaga ttttaacgcg cagttcacac
     8221 tagcaataac ttaatgccga cgcaacagtc gcgaaagatt tgcgaatggc ttggctcaag
     8281 gaccgctgcc gttggcttct ctgcgcccgc ggcagcctca cggaagcggc ggactccgac
     8341 gctggcggga tgctgagttc cggttccggc tgcccgatac ctgccgactt gcgcggcacg
     8401 agatcggcgc ctggttcggc gacgcatcgt gccagagcat gcggctggca caatggtggg
     8461 attcaccgtt gcatttcgga gatgacgatg gaacaagatt gcgcctcgtg cgccagcagc
     8521 tacgagcgcc cgatccggtc tggcgcccgc ggggcggacg cttgggtggc acgatcctga
     8581 tggcagccaa tccggcaacc cgccgcttcg acgccgacct gttgtggcaa aaggcgcagg
     8641 gttccctcgt ggggggccag ttgcaggagg cccgccaagt gctcaccgcc tacaaggcgc
     8701 tgggccgcgg cgacgacgtc catgcgcacc tgctggccgg gcagatcgca ttgatcgagg
     8761 atcgcatccg cgatggcgcc cggaatgtcc tggatgcggc acgtctcgcg cccgacgatc
     8821 ctcacgcgtt ggctgcggtc gcggggatgt tgctgcaact gggtgagaac gtcgccgcgc
     8881 tcgaatgcct ggatcgcccg tcgcttgcag gttgcgaaga cccgttgttg ctgatgcaac
     8941 tggcagcgca ccgcaggcgg ctgcagcagc acgccgaagg gctggcgctg ctggacaagg
     9001 cgcagtccct ggggtgcgac gaccctgccc tccggtacat gcgcggcgaa gccctcctgc
     9061 acagcggccg gctcgaggag gccgaggcgg aactcgcttc ctgcctggcc gatgcgccgg
     9121 agcgtggccg ggtcgcggtg ccactggtcg ggctgcgccg ccagacgccc gagcgcaatt
     9181 acctcgctgc gctggatgcc ggcacccgca tggcgccatc gggaacgagc gaccaggcgg
     9241 cgttcgaatt tgcgcgctac aagacgctcg aagatttggg gcgcaatgac gaggcgtggc
     9301 aggccctcgt gcacggcaac aaactgatgc atgcccggct gccggagcgg ctccggcgac
     9361 cccagccttt gtctgacagg ttcctcgaag cgtggccggc cgcgagtggc gatccggcgc
     9421 ccgctgggga atccggtccg caaccgatct tcatcatcgg catgccgcgg tcgggtacca
     9481 ccgtgctgga acgcatgctc ggaaatcatt ccgaggtcac ggccgccggc gagttgatgg
     9541 atttcgccct gcagatgcac tggacggccg acacccgcaa tctggctggt gaccagttcc
     9601 tgtcgcgcct gccgggcctc gactacggcg aactgggccg ccgctatctc gcgcaaacgc
     9661 ggtggcatgg ccgcggcaaa gcctttttca tcgacaagca gccgccgaac tggatgtggg
     9721 ccgcggccat ccgccacgca ttaccgcgtg caccgatcct gaacctggtg cgcgatccga
     9781 tggatgtctg cttctcgcac tggcgcacgt atttcggcga gagctgcgcg tacagccacg
     9841 acttccagac gctcgcaggc gtttaccgcg actaccggcg cgcgatggcg cactggcacc
     9901 ggatcatgcc gggcgcgatc ctggacgtgc cttacgagga actggtccgc gaccccgacg
     9961 ccaccctgcg aaaggtgttc gacttctgcg ggctggcatg ggaaccaggc tgcgcggaca
    10021 tcacccgcaa tgccgcgccg agcgcgacgc cgagcgcggc gcaggtgcgc tccagcctgc
    10081 acacccgcgg attcggtgag tggcggcgct acgagtctca attgcaatcg ctggccgatg
    10141 cgatagcggc tggcggggaa ttcggctccg gaaacgagga tcgcccggct tcgggttcct
    10201 gataccttgc acgttgtgga tacccctgca gacaatcgat ggtccaggat cgaccggtac
    10261 cttgcaacgc ggcaatttgc cgccgcccgg gccgatctgg aagccatcct ggcgtccgat
    10321 cccgcgtctg cgcccgcaca cctcttgctg agcggcgtcc atagcgccga aaaccgtcca
    10381 cgactcgccg caagccacgc cctcgaggcg gcacgtcatc cgcccgcgga tccgatgttg
    10441 ctgggtgaac tgatcggcgg attgctgaag gtcggcgaga tggtggcggg gcgacgcctg
    10501 ctcgacgatc cggcgatctc cacgagcgcc gatccgaatg tcctggtacg cgcggcccgc
    10561 cagcgaaagg cattgggcga acacaaggcc gcgctcgcgc tgatcgagcg agccgcccgc
    10621 gccggtgcgc gcggcccgac cttcctgttc cagcgcgcga cgcaactgct gttcaacggc
    10681 cggatcgaag aggccgaagc cgacctggag cagtgcatcg cgtccgggtc gccgccgacc
    10741 cgggcctacc tggagctcgc caccctccgc acccagacgg cccagcgcaa ccacctgcgt
    10801 gcgatcaagc tggccatgga tcgtgtcgat ccgaagaccg aggactacgc tttcctagag
    10861 ttcgcgtatt acaaggaact ggaggacctc ggccgctttg gggatgcgtg gcgcgcgctg
    10921 gaacgcggca acgccgtatt gcatgcgctg atgccgtacg acgccgagca cgagtcggcg
    10981 atgttcgatc gcctgatgga agtctgcacc ccagaattcc tgcggtccga aacagacgat
    11041 tcagacgagg gcccccagcc gattttcgtg gtcggactgc cacgctccgg caccaccgtc
    11101 ctggagcgga tcctcggcaa ccactcgcag atcgagtccg ccggcgagct cgttgctttc
    11161 ccgcgcgcgc tggcttggat ggtcgaccat gactcgccgg gcgtgatgga tgacgtcacg
    11221 gtcgagcggc tggtcgacgc ggactggcca gccatcgggc aaaggtatct cgaacagacc
    11281 cggtggcacg cgcgcggcaa cgcgttttac gtggacaagc tgccgcccaa ctggatactg
    11341 gctggcctga tccacaaggc gttgccccac gcgcgcatca tcaacctgat gcgcgagggc
    11401 gtgggtgtct gcttctccaa ttggcgcgcc tgcctcggca cgcatccgga ataccgttac
    11461 gcatatgacc tcggcagcct ggttgcgcac ttccggcaac accggcgctt gatggcgcat
    11521 tggcatcgcg tgctaccggg aaagatcctc gacgtggatt acgcgagtct ggtccgggat
    11581 ccggaagcaa ccgcgcggga gattttcgcg ttttgcggcc tgccgttcga gccgcattgc
    11641 acagacatcg ccggcaacct gtcgccggtg tcgtcgctga gcgcgccaca ggtgcgggag
    11701 cccatccaca cgcggtcttc cgcaagctgg aaaccctacg ccgcgcaact cgagccgctg
    11761 attcgggccc tgtcctcgcc cacggggtaa agaacagcca cgctccagtc tgatgcgcac
    11821 cgaatctcgg tgcgcacatc ggcagcggca ttcgcttgtt cgcgcgccat gcgcgcgaac
    11881 gtcggcagcg atgcccaaca agggacgttc acgctgaacg tggcttgcgc cagcaagcga
    11941 agtcgaagcg tttgccggtc aggttccttc gacttcggcc ctgccggcca tgccaagggc
    12001 gaacggcgtg tagagcatcc cgtcaacgca ttcacttttg cagtccgcag aggcagtcga
    12061 atctcggcca cgccgggagc ttgcccgcaa caccagatgc attagctccc aaaaaaaagc
    12121 ggccccgttt ccggggccgc tgaaggtgca tcgttgcagc tttgcttaga acttctggtt
    12181 gtaccgcacg tagtagaacc ggccaatgtc gaatccgccg tagtacggag cgatgctcga
    12241 gctcggggcg ctgtacagga tcggcccgaa acggtcgaac acgttgttga caccgagcga
    12301 caccgtcgcc tggatcggcg cgatggtata gcgcacctgc aggtcgttga acaccgtgtc
    12361 gccggtgatg ttcaggttgg aaacgacgcc gttctcgtag tgtccggggg tgttgcattc
    12421 cggaccgcct tcggccgtgt tgtcatacga acactgctcc ttgatcgccg agtacaggcg
    12481 cgtggtccag gtggcgcccc agttgcccag cgaccagtcg aggctggcat tgacgcggta
    12541 ccggaaggca ctgccgaagc tgttggagtg gttgagcggc gtgtccggca ggttgtcggg
    12601 cttctggttc tgcagatcgt agtacgtgcc ctggaagttg aacgtgaaca ggccgaacg
//
LOCUS       DASSNH010000223         7940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_774259, whole genome shotgun sequence.
ACCESSION   DASSNH010000223 DASSNH010000000
VERSION     DASSNH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774259"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="hrcA"
                     /locus_tag="VFK00_08030"
     CDS             complement(<1..807)
                     /gene="hrcA"
                     /locus_tag="VFK00_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7592934.1"
                     /translation="MSAAPDQPLDARSRQLLRALIAQYLEEGQPVGSRTLARSAGLDV
                     SPATVRNIMSDLEELGLVASPHTSAGRVPTPRGLRVFVDSLLELKPLPRGAVMKIQRE
                     LPRDSATPRELLGSASTLLSAMTRFVGLVTVPGAVDAPLKQIEFVAIDTRRVLAILVF
                     ADGQVQNRVLNLSQRLESHRLAQAANYLNAHYGGLQLDQIRVRVLAELRAASSELNRL
                     LDTAVEVAAQTFAAEPKADMLVSGQTNLMSSEDGAQIDRLRELFEAFQRKR"
     gene            1009..2685
                     /gene="recN"
                     /locus_tag="VFK00_08035"
     CDS             1009..2685
                     /gene="recN"
                     /locus_tag="VFK00_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7592935.1"
                     /translation="MLTHLSVRNFAVVEGAELEFGPGLTVVTGETGAGKSLLVDALLL
                     LSGARADTGMVRAGCERAEISTEFDLANAPVTRAWLSEQAFDDEDGACRLRRVIAAEG
                     GSRTWINGRTASLAQLSELAATLVEIHGQHEHQALLDRARQLQMLDAFGGNGDAVAKV
                     VELALRHRDIVARERQLSGGDDRSARIDLLRHEIAELERWALPADALADLDARHKRLA
                     NAGKLAEGTSGIADLLDGDAEFAATRSVVRAYTELERLAELDPALHAPLELLDNARIQ
                     LGEAADALSRYAQDLDLDPESYAEADAHLTALHDLSRRHRLPVAELQAHLAGLREELQ
                     ALEDAGESLTRLAAERKQCFADYGIAAAALSKRRIAAATTLGKAVTALMDELGMSGGS
                     FAVDVQPQFSGEPDPQGRERCEFTVSANPGMPLRPLRKVASGGELSRIGLAIEVAALG
                     ADSVATMVFDEVDAGIGGAVAETVGAKLRALGSRCQVLCVTHLAQVAAQGHAHLSVTK
                     IVEGDATRTRIEALDAAGRKEELARMLGGIEIDRETRAHAKKMLEKAQIT"
     gene            complement(2819..3226)
                     /gene="fur"
                     /locus_tag="VFK00_08040"
     CDS             complement(2819..3226)
                     /gene="fur"
                     /locus_tag="VFK00_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HET7592936.1"
                     /translation="MESQELRNVGLKVTHPRIRVLEILEHSHDHLTAENIYKTLLEGS
                     EDIGLATVYRVLTQFEAAGLVVKHNFEGGQAVYELDRGQHHDHMIDVESGKVIEFVNE
                     DIEKLQREIAAKHGYEIVDHSLVLYVRPKKKKS"
     gene            3319..3756
                     /gene="bamE"
                     /locus_tag="VFK00_08045"
     CDS             3319..3756
                     /gene="bamE"
                     /locus_tag="VFK00_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HET7592937.1"
                     /translation="MRKTIPTLLFAMAAVSMLGGCHAIYRPDVQQGNLLIGKNVSELK
                     PGLTKQQVLALLGTPSVVSPFDQNQWNYVATLQRRGGTVKERTLTLYFDNDTLVRTDG
                     DFMKETPQELLRESTKYGPLYPTNLTKEQQKELEKKQKQGGGG"
     gene            complement(3894..4193)
                     /locus_tag="VFK00_08050"
     CDS             complement(3894..4193)
                     /locus_tag="VFK00_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HET7592938.1"
                     /translation="MAENPSIGVSVVYAEADRAFERALRLPAGATVGDAIEASRVRDA
                     RPDIELDPHRIGIFARRATLDTRLRDGDRVEIYRPLKVDPKEARRRRAETSRRLG"
     gene            complement(4186..4614)
                     /locus_tag="VFK00_08055"
     CDS             complement(4186..4614)
                     /locus_tag="VFK00_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HET7592939.1"
                     /translation="MQIHRHALVRYTPEQMFDLVNDVEAYPRRFPWCLGAHVIERDDQ
                     HLVARLDLRFAGVTQHFSTRNTLDRPHSIRMHFVDGPFDWLHGVWSFTPLGDAGCKVA
                     LDLDFEVSNKLTGFAFGLGFKKLADRMVDDFSAEAKRVYG"
     gene            4674..5165
                     /gene="smpB"
                     /locus_tag="VFK00_08060"
     CDS             4674..5165
                     /gene="smpB"
                     /locus_tag="VFK00_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7592940.1"
                     /translation="MASKKDKPGKGTATIALNKRARHEYHIDERYEAGVALEGWEVKS
                     LRAGRINLTEAYALVKGGELYLFGASIPPLLSASTHVIADERRTRKLLLHRAEIDKLI
                     GAVERKGLTLVPLAMYWKNNRAKLEIGIARGKQAHDKRASEKEREWNIEKQRTLRAHN
                     KAG"
     gene            complement(5351..6196)
                     /locus_tag="VFK00_08065"
     CDS             complement(5351..6196)
                     /locus_tag="VFK00_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET7592941.1"
                     /translation="MGSIVWLASYPKSGNTWLRAFLANLVADRPTPLAPNELRGFTDE
                     EASAARFTELAGRPNTELSPEELVALRPQVHALIARQAQGTRLVKTHNMCGAFEGLPL
                     FNWQVTGGAIYVVRNPLDVAVSMTHHFGLTTEQAIERLGDDRVASANDELFVHHIIGS
                     WSTHVRSWADMAARAPDKVLVLRYEDLLEKPAKHFAKAAKLIKLGQDKARVDRAVKHT
                     SFRTLSALEREHGFVEAVDEKTQFFYKGRANQWREALTREQVQRIVTDHREQMQRFKY
                     IPAGY"
     gene            6289..6643
                     /gene="ssrA"
                     /locus_tag="VFK00_08070"
     tmRNA           6289..6643
                     /gene="ssrA"
                     /locus_tag="VFK00_08070"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6801..>7940
                     /locus_tag="VFK00_08075"
     CDS             6801..>7940
                     /locus_tag="VFK00_08075"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET7592942.1"
                     /translation="MRQALTFALALAFASPGVIAAAPPSSAQTANTPTAQLHALFDKA
                     WNEQHAKPPAGTTDYKLLWGDPSAAHEQEQLAQVKANLAALKKIDRAKLSPNDQLSYR
                     LFEYQLQDAIAAAAYPDEAQGLITQLWGPQLAGIDFKDSRYDNAEGYETLVHKLNAVG
                     PYLAAFTGRLQQAIDKGVTQPRAVMERVPDEIKANIADDPTQSPFYAPFKNLPDSIPA
                     DQQAKLQREARDAITRVVNPAYAKFLEFFEDDYLPHARSKAGVSNLPGGSEYYAYLVR
                     HYTTTDVTPKQVHDMGLKLVAQIHGEMEAVFRQIGFKGSYKAFVQHLRTDPKFYYKDP
                     DDLLEAYRAAAKRIDPYLTDVLSLWMQPRQPWGVRVIPASLAPNTY"
BASE COUNT         1419 a         2638 c         2583 g         1300 t
ORIGIN      
        1 gcgtttgcgc tggaacgctt cgaacagctc gcgcagccgg tcgatctgcg caccgtcctc
       61 ggacgacatc agattggtct ggccgctgac cagcatgtcc gccttcggct ccgcggcgaa
      121 ggtctgggcg gcgacctcga cggcggtatc cagcaggcgg ttcaattcgc tgctggcggc
      181 gcgcagttcc gccagcaccc gcacccggat ctggtccagt tgcaggccgc cgtaatgggc
      241 gttgaggtaa ttcgccgcct gcgccaggcg atggctctcg aggcgctggc tgaggttcag
      301 cacgcggttc tgcacctggc catccgcgaa caccaggatc gccagcacgc gccgcgtgtc
      361 gatcgccacg aattcgatct gctttagcgg cgcgtccacc gcacccggaa ccgtcaccaa
      421 cccgacgaac cgggtcatcg ccgaaagcag cgtcgaggcg ctgccgagca actcgcgcgg
      481 cgtcgccgaa tcgcgtggaa gctcgcgctg gatcttcatg accgcgccgc gcggaagcgg
      541 cttcaattcc agcaggctgt cgacgaacac ccgcaagcca cgcggcgtcg gcacccgccc
      601 ggccgaggtg tgcggcgagg cgacgagccc cagttcctcg aggtccgaca tgatgttgcg
      661 gaccgtggcc gggctcacgt ccaggccggc ggagcgggcc agggtccgcg agccgaccgg
      721 ctggccttcc tccaggtact gcgcgatcag ggcgcgcaac aattggcggg aacgggcgtc
      781 gaggggctgg tcgggggctg cggacatggc tgcgaaatca tcaatacggg tggcgggtgc
      841 gaaatgcaag tgaaaggcgg cggggagaat ggatcgtggg tgtgcagttc tgtggcctcg
      901 ctcacaggac ggacgtctgg tccggcgatg gggctccggt ttcgcgcccg actccccgcc
      961 cccaatcccc gcttcgctag aatcaccgct ccgaccgagc gccaccccat gctcacccac
     1021 ctctccgtcc gcaatttcgc cgtggtcgaa ggtgccgaac tcgaattcgg gccaggcctc
     1081 accgtggtga ccggggaaac cggcgccggc aagtcgctgc tggtcgatgc gttgctgctg
     1141 ctgtccggcg cgcgtgccga caccggcatg gtacgcgccg gctgcgagcg cgcggaaatc
     1201 tcgaccgaat tcgacctcgc gaacgcgcct gtcacgcgcg cgtggctctc cgaacaagcc
     1261 ttcgatgacg aggacggcgc ctgccgcttg cgccgcgtga tcgccgcgga aggcggctcg
     1321 cggacgtgga tcaacgggcg cacggcatcg ctcgcgcaac tgtcggaact cgccgccacg
     1381 ctggtggaga tccacggcca gcacgagcac caggcgttgc tggatcgtgc acggcagttg
     1441 cagatgctgg acgcgttcgg cggcaatggc gatgcggtgg ccaaggtcgt cgaacttgcg
     1501 ctgcggcatc gcgacatcgt ggcgcgggaa cgccaactgt cgggtggtga cgaccgctcc
     1561 gcccgcatcg atctcctgcg ccacgaaatc gcggaactgg aacgctgggc gctgccggcc
     1621 gatgcgctcg ccgacctcga cgcccgtcac aagcggctgg cgaatgccgg caagctggcg
     1681 gagggcactt ccggcatcgc cgacctgctg gatggcgacg cggaattcgc ggcgacccgc
     1741 tccgtggtcc gtgcgtatac ggaactggaa cggctggccg agctcgatcc cgcgctgcat
     1801 gcgccactgg aacttctcga caacgcgcgc atccagctcg gcgaggccgc ggacgcgctg
     1861 tcgcgctacg cgcaggacct cgacctcgac ccggaaagtt acgccgaagc cgatgcacac
     1921 ctgaccgccc tgcacgatct ttcgcgtcgc caccgcctgc ctgtcgcgga actgcaagcg
     1981 catctcgccg ggctgcgcga ggaactgcag gcgctggaag atgccggcga gtcgctcacc
     2041 aggctggccg ccgaacgcaa gcaatgtttc gccgactacg gcatcgccgc cgccgcgctg
     2101 tccaagcgcc gcatcgccgc cgcgacgacg ctcggcaagg cggtcaccgc gctgatggac
     2161 gaactcggca tgtccggcgg gtcgttcgcc gtggacgtgc aaccgcagtt ttccggcgaa
     2221 cccgatccgc agggccgtga gcgctgcgag ttcacggtca gcgcgaaccc cgggatgccg
     2281 ctgcggccgc tgcgcaaggt cgcctccggc ggcgaactgt cgcggatcgg ccttgcgatc
     2341 gaagtggccg ctctcggcgc cgacagcgtc gcgacgatgg tgttcgacga ggtcgacgcc
     2401 gggatcggcg gcgcggtggc cgaaacggtg ggcgcgaaac tgcgcgcact cggttcacgc
     2461 tgccaggtgc tttgcgtcac ccacctggcg caggtcgcgg cgcaggggca tgcccatttg
     2521 tcggtcacca agatcgtcga gggcgacgcc acccgcaccc ggatcgaagc tctggatgcc
     2581 gccgggcgca aggaagaact cgcgcggatg ctcgggggca tcgaaatcga ccgcgaaacc
     2641 cgggcgcatg cgaagaagat gctggagaag gcgcagatta cgtaggccgt ggccgctgtt
     2701 gtgccgggtc gcggccatgg gccgctctca ttcgccttcg gccgcacgta gcggcgtagc
     2761 gtgcgccaac gtgcggtgga aggggccgcg ccgatgccac cctgcgccgg gtcccgaatc
     2821 aggatttttt ctttttgggt cgaacgtaca acacgaggct gtggtcgacg atctcgtagc
     2881 cgtgcttggc agcgatctcg cgctgcaact tctcgatgtc ctcgttgacg aattcgatga
     2941 ccttgccgct ctcgacatcg atcatgtggt cgtggtgctg gccacgatcc agttcgtaca
     3001 ccgcctggcc gccttcgaag ttgtgcttga ccacgaggcc ggccgcctcg aactgggtca
     3061 gtacgcggta caccgtcgcc aagccgatgt cctcggagcc ctcgagcagg gtcttgtaga
     3121 tgttctcggc cgtgaggtgg tcatggctgt gctcgaggat ttccagcacg cggatgcgcg
     3181 gatgggtcac tttcaacccg acgttgcgca gttcctggga ttccataggc tgaactccgg
     3241 caagatggtg ctgtctctga tccggaccag gacccgggct agtgtatcat tcgcggtccg
     3301 gaatccggct gaccacgaat gcgcaaaacg atccctaccc tcctgttcgc gatggcggcc
     3361 gtgtcgatgc tcggcggctg tcacgcgatc taccggcccg acgtccagca gggcaacctg
     3421 ctgatcggca agaacgtcag cgagctgaaa cccggcctga ccaagcagca ggtgctggcg
     3481 ctgctcggca cgccgtcggt ggtcagcccc ttcgaccaga accagtggaa ctacgtcgcc
     3541 acgctgcagc gccgcggcgg cacggtgaag gaacgcacgc tgacgctgta tttcgacaac
     3601 gacaccctgg ttcgcaccga tggcgacttc atgaaggaga cgccgcagga gttgctgcgg
     3661 gaaagtacca agtatggccc gctctatccg acaaacctga ccaaggaaca acagaaggaa
     3721 ctggagaaga agcagaaaca gggtggtggc ggctagccgc ggccttcgtt ctgcggagca
     3781 aatcattcgc aggatggccg ctcggtccgc ggcaaatcgg atggacgtgg ctacagcgaa
     3841 atcgtagttg ccacttgcgg gcatgtcgtg gggcttctcc ggactcgtcc ggatcagccc
     3901 aacctacggc tggtttccgc ccgccggcgc cgcgcttcct tcggatcgac cttgagcggc
     3961 cggtagattt ccacgcggtc gccatcgcgc agccgggtgt cgagcgtcgc cctgcgcgcg
     4021 aagatgccga tgcgatgggg atcgagttcg atgtcgggcc gcgcgtcgcg gacgcgcgac
     4081 gcctcgatcg catcgccgac cgttgcgccc gccggcagcc gcaacgccct ttcgaacgcg
     4141 cggtcggctt cggcatacac caccgaaacg ccgatgctcg ggttttcagc catacacgcg
     4201 cttggcctcg gcgctgaaat catcgaccat ccggtcggcg agcttcttga agccgagccc
     4261 gaacgcgaag ccggtcagct tgttgctgac ctcgaagtcg aggtccagcg cgaccttgca
     4321 gccagcgtcc ccgagcgggg tgaaagacca caccccgtgc agccagtcga agggaccatc
     4381 gacgaaatgc atgcggatgc tgtgcgggcg gtccagcgtg ttgcgggtcg aaaagtgctg
     4441 ggtcacccct gcaaagcgca gatcgagccg ggccacgagg tgctggtcgt cgcgttcgat
     4501 tacgtgcgcg cccaggcacc aggggaaccg cctcgggtaa gcttccacgt cgttgacgag
     4561 gtcgaacatc tgttccggcg tgtagcgaac cagcgcatgg cggtggatct gcacgaagac
     4621 tccttcaagc gcagcgcaag ccgcgcgcgc atacggtccc cattgtaccg agcatggcca
     4681 gcaaaaaaga caaaccgggc aagggcacgg cgacgatcgc gctgaacaag cgcgcgcgcc
     4741 acgaatacca catcgacgag cgctacgagg cgggtgtcgc gctggaaggc tgggaggtca
     4801 agagcctgcg cgccgggcgc atcaacctca ccgaggccta cgcgctggtc aaaggcggcg
     4861 aactgtacct gttcggcgcg tcgattccgc cgctgctgtc ggcctcgacc cacgtgatcg
     4921 ccgacgaacg ccgcacccgc aagttgctgc tgcaccgcgc cgagatcgac aagctgatcg
     4981 gcgcggtcga gcgcaagggg ctgacgctcg tcccactcgc gatgtactgg aagaacaacc
     5041 gcgcaaagct cgagatcggc atcgcgcgcg gcaagcaggc gcacgacaag cgcgccagcg
     5101 agaaagagcg cgagtggaac atcgagaagc agcgcaccct gcgggcgcac aacaaggctg
     5161 gatagctcga ctccacctcg ggccgttccg ctcgggaaag gatggttgcg tgagtattgg
     5221 ggagagccat ccccctcgat cccccttcgt tccgaagggg aggaaaagcg cgaaagctat
     5281 cccccttcgg aacgaaaggc gccgcgccgc aggcgccagg tggatgcact cccagcgacg
     5341 agatgcgccc tcaatatccc gccgggatgt acttgaaccg ctgcatctgc tcgcggtgat
     5401 cggtgacgat tcgttgaacc tgctcgcgcg tgagcgcttc gcgccactgg ttcgcgcggc
     5461 ccttgtagaa gaattgggtc ttctcgtcca ccgcctccac gaaaccgtgc tcccgctcca
     5521 gcgccgacag ggtccggaac gacgtgtgct tcacggcgcg atccacccgc gccttgtcct
     5581 ggcccagctt gatcagcttc gcggccttgg cgaagtgctt cgccggtttc tccagcaggt
     5641 cctcgtagcg tagcaccagc accttgtccg gtgctcgcgc ggccatgtcg gcccagctgc
     5701 gaacgtgggt cgaccaggaa ccgataatgt gatggacgaa cagttcgtcg ttcgccgaag
     5761 ccacccggtc gtcgcccagc cgttcgatcg cctgctccgt ggtcaggccg aaatggtggg
     5821 tcatgctgac ggccacgtcc agcggattgc gtaccacgta gatcgcaccg cccgtcacct
     5881 gccagttgaa caacggcagg ccttcgaacg cgccgcacat gttgtgggtc ttgaccaacc
     5941 gcgtgccctg cgcctggcgc gcgatcagcg cgtggacctg gggacgcagg gcgaccagct
     6001 cttccgggga cagctcggta ttcggccggc ccgctagctc cgtgaaacgc gcggcactcg
     6061 cctcctcgtc ggtgaaaccg cgcaattcgt tcggcgccag cggcgtgggg cgatcggcga
     6121 ccagattcgc caggaacgcc cgcaaccagg tgttgccgga tttcggatag gaggccagcc
     6181 agacgatgct tcccatgaag cggcaaccct tgcaatgaac gaaccccgca tgatgccagc
     6241 cgccgcgatt gatttcctgc atccgggccc gcatcatggg tgttccccgg gggcgacctg
     6301 gcttcgacgg gggcagtgaa gtagcctgga gcatgccgag ggcgtcatct tcctcgttaa
     6361 tccggtggca aaaaactaga cgcgaacgac gacgtctacg ctctggccgc ttaaggccta
     6421 gccccgaacc catcttgtgc ctgtgctcgt gggtgtaggg tcaccatcac gggatcgccc
     6481 ctggttgccg cgggtcggcc attggctaaa acaaaacctg ctggtttcgc ggtgcgcttt
     6541 gcccagcgtg ctgccgcggg acgagaccga acgctgagct aagcatgtag cgccgggcgc
     6601 ggagcgcctt cggacggggg ttcgattccc cccgcctcca ccagacacga agagccgccc
     6661 ttgtggcggc tttttcgtgt ctggcgaaga tgggtgcgga attgagcctc gttcaacaat
     6721 tcattcccgc caacttccgg cacacgtccc gcatttcacc ctggggctat agtcgaagtt
     6781 cgacttccgg ggaatccacg atgcgtcagg ccctcacctt cgcccttgcc cttgccttcg
     6841 cttcgcccgg tgtcatcgcc gccgcgccgc cctcatcggc gcagacggcg aacaccccca
     6901 ccgcgcaatt gcacgcgctg ttcgacaagg cctggaacga gcaacacgcc aagccaccgg
     6961 cgggcaccac cgattacaag ctgctttggg gcgacccgag cgccgcgcac gagcaggaac
     7021 aattggcgca ggtcaaggcg aacctggccg cgctgaagaa aatcgaccgc gcgaagctgt
     7081 cgccgaacga ccagttgagt taccgcctgt tcgaatacca gttgcaggac gcgatcgcag
     7141 ccgcggcgta cccggacgag gcacaaggcc tgattacgca attgtggggg ccgcagctcg
     7201 ccggcatcga tttcaaggat tcccgctacg acaacgccga gggctacgaa acgctggtcc
     7261 acaagttgaa tgccgtgggg ccgtatctcg cggcgttcac cgggcggctg caacaggcga
     7321 tcgacaaggg cgtcacccag ccgcgcgcgg tgatggaacg cgtcccggat gagatcaagg
     7381 ccaacatcgc cgacgatccg acgcagagcc cgttctacgc gccgttcaag aacctgccgg
     7441 attcgattcc cgccgaccag caggcgaagc tgcaacgcga agcgcgcgat gcaatcaccc
     7501 gcgtggtgaa tcccgcctac gcgaagttcc tggagttctt cgaagacgat tacctgccgc
     7561 acgcgcgcag caaggccggc gtgtcgaacc tgccaggcgg atcggagtac tacgcgtacc
     7621 tggtgcgcca ctacaccacc accgacgtga cgccgaagca ggttcatgac atgggcctga
     7681 agctggtcgc gcagatccac ggcgaaatgg aggccgtgtt caggcagatc ggcttcaaag
     7741 gcagctacaa ggcattcgtg cagcacctgc gcaccgatcc gaagttctat tacaaggatc
     7801 cggacgactt gctggaggcc taccgcgccg ccgccaagcg catcgacccg tacctcaccg
     7861 acgtgttgag cctgtggatg cagccgcgcc agccgtgggg cgtgcgcgtg atcccggcgt
     7921 cgctcgcgcc caacacctac
//
LOCUS       DASSNH010000224         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1778038, whole genome shotgun sequence.
ACCESSION   DASSNH010000224 DASSNH010000000
VERSION     DASSNH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778038"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFK00_08080"
     CDS             complement(<1..945)
                     /locus_tag="VFK00_08080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7592943.1"
                     /translation="MAVMTDSQNASALLGAEGPFACEVPNFAPRETQQRMADAVTAAV
                     ESGGSLVVEAGTGTGKTFAYLVPALLSGKRVIVSTGTRNLQDQLFHRDLPRVLSILGI
                     KADVALLKGRANYLCRYRLQQTVESGQFKSRAQATDLQEIRAWGSRTRAGDRAELAEV
                     PEASPLWPRVTSSAENCLGTECPLHDECFVFRARRRAQEADLVVVNHHLLLSDLALKR
                     EGFGAILPEADAYILDEAHQLPELAGQYFSIGISARQVAELAADTLAECAGQPGALAL
                     LQPALDGLNDAVRKARLAFDTLPRRGAFAQLEADAGAAE"
     gene            complement(1451..1843)
                     /locus_tag="VFK00_08085"
     CDS             complement(1451..1843)
                     /locus_tag="VFK00_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7592944.1"
                     /translation="MDRFTGGCLCGNVRMVAAGRPYRVGICHCLDCRKHHGALFHASA
                     IFPRDAVTVEGETREYAGRFFCPRCGSSVFGRSADEIEVNLGSLDAPDQLQPTYELWT
                     IRRESWLPPFPLARRYERDREATSRPED"
     gene            1968..2312
                     /locus_tag="VFK00_08090"
     CDS             1968..2312
                     /locus_tag="VFK00_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7592945.1"
                     /translation="MQPETPAFPDAMVVIYRWRLRPGMEATFTQAWSRVTELLRARGS
                     LGSRLHRGAEGIWYGYAQWPSAKARADAFAPGPVDEAATAQMRSAIAERFPEIILEPV
                     ADYLVPLRHHQA"
     gene            2379..2576
                     /locus_tag="VFK00_08095"
     CDS             2379..2576
                     /locus_tag="VFK00_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592946.1"
                     /translation="MNRKIRRYCTGNGAGMAAGIAIGIALGTAMDNFAIGVCIGVAIG
                     VSFESGRKKPRKSTGKDGKGG"
     gene            2660..2842
                     /locus_tag="VFK00_08100"
     CDS             2660..2842
                     /locus_tag="VFK00_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592947.1"
                     /translation="MSASTQLRWKAAAFGAAIGIALGAGNGVVFGNIPLGAGAGLVLG
                     VGLGLVIGSLRAKRRA"
     gene            2911..3462
                     /locus_tag="VFK00_08105"
     CDS             2911..3462
                     /locus_tag="VFK00_08105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HET7592948.1"
                     /translation="MQTERQKMLAGELYDPFDPELVAARERARSLCAALNATAESQRD
                     ERRRLLGGLFGSGGDTVWMQPPFYCDYGSNIRLGARVFFNFNCTVLDVCEVRIGDYTL
                     FGPGVQILTPLHPRDARLRREQEYGKPIEIGADVWIGGGALVLAGVRIGSGSIIGAGS
                     VVARDIPDGVLAVGNPCRVVGKA"
     gene            complement(3494..3796)
                     /locus_tag="VFK00_08110"
     CDS             complement(3494..3796)
                     /locus_tag="VFK00_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7592949.1"
                     /translation="MALICDSDPDGERITLHISDRFDFSVHRGFHEACLARPRARSYL
                     VDLEGVTSMDSSALGMLLLLREHAGGDDADIRILNAGRDLRNTFRVAGLDRLVTLG"
     gene            complement(3810..>4685)
                     /locus_tag="VFK00_08115"
     CDS             complement(3810..>4685)
                     /locus_tag="VFK00_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7592950.1"
                     /translation="GELGSGFDGLSRKNTAQQQLLQQVIESSGSEGGSVQDFIRRTGD
                     LLEHFVALVVHLSRDSLRIVYRIDGMSREMDAIFGLLKNVDTIAEETNLLALNAAIEA
                     ARAGESGRGFAVVAAEIRNLARHSNQFNEQIGSHVERSRRSMEQLRELVGAMASQDMS
                     VALATKGDIDAMMTRLASNDRRSAEVTAQVASIAQGLAGDVATTVRSLQFEDILSQLM
                     GQTNARLLDLQDVADRCSEDLESMLRLGAEPARLQEHAERMRGRLQRQRDRSHVRARG
                     PAMQTSMSAGEIDLF"
BASE COUNT          768 a         1555 c         1579 g          783 t
ORIGIN      
        1 ttcggcggcg ccggcatcgg cttccagttg cgcgaatgcg ccgcgccgcg gcagcgtgtc
       61 gaacgccagc cgagccttgc gcaccgcatc gttcaggcca tccagcgccg gctgcagcaa
      121 tgccagcgca cccggctgcc cggcgcactc ggcgagcgtg tcggcggcca gttcggctac
      181 ctgccgcgcg ctgatcccga tcgagaaata ttgccccgcc agctccggca actggtgcgc
      241 ctcgtcgagg atgtaggcat cggcttccgg caggatcgca ccgaagcctt cgcgcttcag
      301 tgcgagatcg gacagcagca gatggtggtt cacgacgacg agatcggctt cctgtgcgcg
      361 ccggcgtgcc ctgaacacga agcactcgtc gtggagcggg cattcggtgc caaggcagtt
      421 ttccgcgctg gacgtcaccc gcggccacag cggagaagcc tccggcacct ccgcgagttc
      481 cgcgcgatcg cccgcgcggg tacgggaacc ccatgcgcgg atctcctgca agtcagtggc
      541 ttgtgcgcgg gatttgaact gccccgactc gacggtttgt tgcaggcgat atctgcaaag
      601 gtagttggcg cggcctttca gcagggcgac gtctgccttg ataccgagga tcgacaacac
      661 ccgcggcagg tcgcggtgga acaactggtc ctgcaggttg cgggtgccgg tcgaaacgat
      721 cacccgtttg ccggacagca gcgccggcac caggtacgcg aaggtcttgc cggttccggt
      781 accggcctcg accaccagcg aaccgccgga ctcgacggct gcggtgaccg cgtcggccat
      841 ccgttgctgc gtctcgcgcg gcgcgaagtt cggcacctcg cacgcgaacg gcccctccgc
      901 gcccagcagc gcggaggcat tttgcgaatc ggtcatcacg gccatcgagc aagtatcgcg
      961 gaatggagcg gggcgcgaaa tgcgctggcg gcgatcccgc gcaccaggcg gacgcatcag
     1021 cacttgcagg tctcgcttgg ggggacaaat gctcgcacga caatccttcg caaatctcgt
     1081 ccgtgccgcg tgcgatactc gcccgacacg aggggagaac cagcatggct gaacaaacgg
     1141 attgcttgcg ctgtcacgtg ccgatggagg ccggtttcgt tgcggaccgc acctacggcg
     1201 gttgggtcga aacgcagtgg gcaccgggac tcccggagcc acactggtgg ggcatggcta
     1261 cacccaagag tcgcattcct gtagtgacct tgcggtgtcc gaaatgcgga gcactcgagt
     1321 cgttcgcgcg ccccgcatga gtgaagccgg cttgtcatgc gccgcgaacg cgagccgatg
     1381 attcatgtgc gcccagccga ccgattcagc aggccgccgg ttcgtccgaa cgcgtgctgg
     1441 gccctgcccg ctagtcctcg gggcggctcg ttgcttcgcg gtcgcgctcg tatcgcctcg
     1501 ccaacggaaa tggcgggagc caggattcgc gacgaatcgt ccagagttcg taggtgggct
     1561 gcagttggtc gggggcgtcc agcgacccga ggttcacttc gatttcgtct gcagatcggc
     1621 caaaaacgga tgaaccgcag cgcgggcaga aaaatcgccc cgcgtactcc cgcgtctccc
     1681 cttcgaccgt caccgcatcc cgcgggaaga ttgcggaggc gtggaacagc gcgccgtgat
     1741 gcttgcgaca gtcgaggcaa tggcaaatgc cgacccggta cgggcgtccc gcagcgacca
     1801 ttcgaacgtt gccgcacagg caaccgccgg tgaatcgatc cattctggcc tcctctaaac
     1861 tcgggcttct tgccagcgca cccatcggcg cggccgcgac gatggcgtgg gcagcgcctt
     1921 ggcgaatgca ttctattccg cgaccgtctg gtcgcttagg agctccggtg cagcctgaaa
     1981 cacctgcatt tcccgatgcc atggtggtca tctaccgctg gcgcctgcgt cccggcatgg
     2041 aggcgacgtt cacgcaagcc tggtcccgcg tgacagagtt gctgcgggcg cgcggatcgc
     2101 tggggtcccg cctccaccgc ggcgccgagg ggatctggta cggctacgcg cagtggccga
     2161 gcgccaaggc gcgcgcagat gccttcgcgc ccggtcccgt ggacgaggcg gccactgcac
     2221 agatgcggtc cgccatcgcg gaaaggtttc ccgagatcat cctcgaaccc gttgccgact
     2281 acctcgtgcc gttgcggcac caccaagcct gaccccggca ccctgcgatc cgttatcctc
     2341 gcgaccatgc acccggcacg cttccaaaac ggtccgcgat gaacaggaaa atccggcggt
     2401 actgcacggg caatggcgcc ggcatggcgg ccggcatcgc gatcgggatc gcgctcggca
     2461 cggcgatgga caacttcgcg atcggcgtgt gcatcggggt tgcgatcggt gtgtcgttcg
     2521 aaagcggccg gaaaaaaccg cggaaaagca caggcaagga tggcaagggc ggatagcagc
     2581 agggagcgcg ccgcctgacc ctgagcttca ccagttccgc cactgcaggc agtccaatca
     2641 ggatccgggg agggatcaga tgagcgcaag cacgcaactt cgttggaaag ccgcggcgtt
     2701 cggtgccgcc atcggcattg cgctgggtgc gggcaatggc gtcgttttcg gcaatattcc
     2761 tctgggtgcc ggcgccgggc tggtcctcgg cgtcggactc gggctggtga tcggctcact
     2821 gcgcgcgaag cgcagggcgt aaccggcgaa cgctgccgcg cgatcgcgga gccgcaacaa
     2881 gcccggcgcc agcgtgggat catggacgcc atgcagaccg agcgccagaa gatgctcgcg
     2941 ggcgaactgt atgacccgtt cgatccggag ctggttgccg cgcgcgaacg cgcccggtcg
     3001 ctgtgcgcgg cattgaacgc gactgcggaa tcgcaacggg acgagcgacg gcgcctcctc
     3061 ggcgggctgt tcggcagcgg cggcgacacc gtctggatgc aaccgccgtt ctactgcgac
     3121 tacggttcga acatccgcct cggcgctcgg gtgttcttca atttcaactg caccgtcctc
     3181 gacgtctgcg aggtccggat cggcgattac acgctgttcg gtccaggcgt ccagatcctc
     3241 accccgctgc atccgcgcga cgccaggctg cggcgggagc aggaatacgg caagccgatc
     3301 gagatcggcg cggatgtctg gatcggtggc ggcgcgctgg tcctggccgg cgtccgcatc
     3361 ggctcgggca gcatcatcgg tgccggcagc gtcgtggcgc gggacattcc cgacggcgtg
     3421 ctggcggtcg gcaatccatg ccgggtggtc ggcaaggcgt agggtttcga gcttccccga
     3481 agtcccggcc gcctcagccc agcgtcacca accggtcaag acccgccacg cggaacgtgt
     3541 tgcgcaaatc cctgcccgca ttgaggatcc ggatgtcggc atcgtcgcca ccggcgtgct
     3601 cgcggagcag cagcaacatg ccgagcgccg agctgtccat gctggtgacg ccctcgaggt
     3661 cgaccaggta gctgcgcgcg cgcgggcgcg ccaggcaggc ttcgtggaag ccgcggtgca
     3721 cggagaagtc gaagcggtcg ctgatgtgca gcgtgatgcg ctcgccgtcg ggatcggaat
     3781 cgcagatcag ggccatcgca cgccttcctc tagaacaggt cgatttcgcc ggcgctcatc
     3841 gaggtctgca tcgccggccc cctcgcgcgc acatgggaac gatcgcgctg gcgctgcagg
     3901 cgaccgcgca tccgctcggc gtgttcctgc agtcgcgcgg gctcggcgcc caggcgcaac
     3961 atcgactcga gatcctcgct gcaacgatcg gccacgtcct gcaggtcgag gaggcgggca
     4021 ttggtctggc ccatgagctg gctcaggatg tcctcgaatt gcagcgagcg cacggtcgtg
     4081 gcgacgtcgc cggccagccc ctgcgcgatc gatgccacct gcgccgtgac ctcggcgctg
     4141 cggcgatcgt tgctggcgag gcgcgtcatc atcgcatcga tgtcgccctt ggtcgccagc
     4201 gcgacgctca tgtcctgcga agccatcgcg ccgaccagct cgcggagctg ctccatgctg
     4261 cggcgcgaac gctcgacgtg gctgccgatc tgttcgttga actggttgga atggcgcgcg
     4321 aggttgcgga tttcggcagc caccacggcg aagccgcggc ccgactcacc cgcgcgcgcc
     4381 gcttcgatgg cggcgttcag cgccagcagg ttggtttcct cggcgatcgt gtcgacgttc
     4441 ttcaacaggc cgaagatggc atccatttcg cgcgacatgc cgtcgatgcg atagacgatc
     4501 cggaggctgt cgcgcgaaag gtgcacgacc agcgccacga agtgctccag caggtcgccg
     4561 gtcctgcgga tgaaatcctg cacgctgccg ccttcgctgc cgctgctctc gatcacctgc
     4621 tggaggagtt gctgctgggc ggtgttcttg cgggatagtc cgtcgaaacc gctgcccaac
     4681 tcgcc
//
LOCUS       DASSNH010000225         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1643291, whole genome shotgun sequence.
ACCESSION   DASSNH010000225 DASSNH010000000
VERSION     DASSNH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643291"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFK00_08120"
     CDS             complement(<1..479)
                     /locus_tag="VFK00_08120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7592951.1"
                     /translation="MPSDEPLADAALLIIDVINDFAFPGGEQLVRQMDPVVEAIDALR
                     LRADALQRPVIHVNDNFGRWRSNFAQVIEWCRREGGRGADIATRLSPRQSDYFVLKPG
                     QSGFYQTPLPALLEYLKVSRLVLTGVAGDQCVMTTAMDAHIRDYHLWVPANATASLTP
                     "
     gene            complement(755..1720)
                     /locus_tag="VFK00_08125"
     CDS             complement(755..1720)
                     /locus_tag="VFK00_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET7592952.1"
                     /translation="MLTIHCDGKAQSTTWQAGNPIPAEAAWLDLCQPEGGETEACQDA
                     AGVRLPKREDIRGIGLAGRNRCLGDALFLQVSSFADAEDDKKRPTPLALVLTPRLLVS
                     QRYAPSRTFDIAASQWHETQRDDGPASALAEIIETMAERTAQSMQKVADEVADLSRKV
                     FTGKREDTRVLRRWLVHVGHLEARLAATRGSLLGITRVVTFICENDPDWIPEAQGSRI
                     SMVRNDLKALDEFDEQLTGKLQFLLDSIFGFISVNQNSVMKLFTVVAVAAFPPTVLAG
                     IWGMNFKYMPALSQPWGYAMALAAIALSILVPLAIFKWRGWLSRD"
     gene            2159..3997
                     /locus_tag="VFK00_08130"
     CDS             2159..3997
                     /locus_tag="VFK00_08130"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HET7592953.1"
                     /translation="MHTKLHLSALVVALGMAMAGAAGAAAPAPGTAMAHPTRLAQGDV
                     VAGPLALSQPMHIVIGLKLRNKAELDAYIAKPGFKPLSSAQFAQRYSPTAAQAQKVAD
                     FLRQRGFTNIRIAPNRLLVSGDAPASSVQSAFDTSLVSVRTHDGRQAYANTRAATVPS
                     SLAGMINGVMGLQTVHIPHLLHQKTGGRAGTNAVTGHSPTEFPSIYGATSLPTAAGVT
                     VGILAEGKLTQTINSLNTFTSQNGYPTVTTRIVGTSTRDTAGTLEWELDSQTIVGAAG
                     GEVGSIVFYMMPNISTDTAVAEGINLAVTDNTAQVVNGSLGVSEVDAQNDGTFASSDA
                     LLEQGVAQGQTFSFSTGDDGASSCFALHQDAACWPATSQYVVAVGGTTLDASTGPDAT
                     WNQEVVWGGAGGAESEVEPKPSWQTLWSGDHRAAPDVSFDASPNSGALIIDGGGTVQV
                     GGTSLSSPMFAGFWARVVAGKGNLGFAAPLLYPLSASDFHDITQGSNGNAAAPGFDLA
                     SGRGSMVWSEVYQELSGGGGDEPPVASFTVKTRGKFVRLTDTSTDPDGTIASRMWDLG
                     DGRTSTRQKLTYRYASPGTYTVTLTVTDNDGESSSTSQDVTIGGGQ"
     gene            4344..>5552
                     /locus_tag="VFK00_08135"
     CDS             4344..>5552
                     /locus_tag="VFK00_08135"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HET7592954.1"
                     /translation="MPSQSPSNRGGQDRLARLAAERVVIEEVWPEIDGGRHPIKRVVG
                     DELEVWADIFCDGHERLAACICWREVGARKWREAPMQLVNNDRWRGTFTVDRNTRYVY
                     TIEAWRDRFATWREDFLKKRDAGQDIALELLEGRELVHAAAEGAPRSFGSMVKNLDTA
                     ADDAQLQALLLDDELLERMRHEGTRTNATRYRHELEVVVDRPAARFGAWYELFPRSAS
                     NDAHRHGNFDDVIARLPYVRELGFDVLYLPPIHPIGVSHRKGRNNALEAAQDDPGSPW
                     AIGSAGGGHTAIHPELGTLEDFRRLIAAAKKQGIEIALDFAIQCSRDHPWIKEHPEWF
                     DWRPDGTIKYAENPPKKYQDIVNVHFYRDALPSLWLALRDVVRFWIDQGVRIFRVDNP
                     HTKPVPFWEWL"
BASE COUNT          978 a         1809 c         1847 g          918 t
ORIGIN      
        1 ggcgtgagcg atgcggtcgc attggccggt acccacaggt ggtagtcgcg gatgtgggcg
       61 tccatcgcgg tcgtcatcac gcattggtcg ccggccaccc cggtcagcac cagccgcgaa
      121 accttcaggt attcgagcag cgccggcagc ggtgtctggt agaaacccga ctgaccgggc
      181 ttcagcacga agtaatcgct ttgccgtggc gacagccggg tcgcgatgtc ggcgccccgg
      241 ccgccttcgc gccggcacca ctcgatcact tgcgcgaaat tggaccgcca ccggccaaag
      301 ttgtcgttga catggatgac cggccgctgc agcgcatcgg cgcggagccg caaggcgtcg
      361 attgcttcga cgaccggatc catctgccgc accagttgtt cgccgcccgg aaacgcgaag
      421 tcgttgatca cgtcgatgat caacaatgcc gcgtcggcaa ggggttcgtc ggatggcatg
      481 gcggaaatcc cggtgcggag ccacattgtg ggcccggtgg cgtgtgggtc gtgtgactgc
      541 ccgaacgggc aggggagttc tccaccgacg atccgacagg atcgggaacc cggcccggtt
      601 gggcgcacac cccactcttt gccttccccg aaagcagggc cggacgtcac cccaagctac
      661 aaaatcgcct ggccccgcgc gggagcgcgt cgcgcccgca accgaacaag ggcgtccgcc
      721 gggatgatgg cagcgtccac cgctcgcatc gtgcctaatc ccgcgacagc cacccccgcc
      781 atttgaaaat ggccaggggc accaggatgc tgagggcgat ggcggcgagc gccattgcgt
      841 acccccaagg ctgcgaaagt gccggcatgt atttgaaatt catgccccag atgccggcca
      901 gcaccgtggg cggaaacgcg gcgaccgcga ccacggtgaa cagcttcatc acgctgttct
      961 ggttgacgct gatgaaaccg aagatcgaat ccagcaggaa ctgcagcttg cccgtcaact
     1021 gctcgtcgaa ttcatcgagc gccttgaggt cgttgcggac catcgagata cgcgaaccct
     1081 gggcttccgg gatccagtcc ggatcgtttt cgcaaatgaa ggtcacgacg cgggtgatgc
     1141 cgagcaggga cccgcgggtc gccgcgagtc gcgcctcgag gtgtccgacg tgcaccagcc
     1201 agcggcgcag gacgcgggtg tcttcgcgct tgccggtgaa gaccttgcgg gagaggtcgg
     1261 ccacttcgtc cgcgaccttc tgcatgctct gggcggtgcg ctcggccatg gtttcgatga
     1321 tctcggcgag cgcgctcgcg gggccgtcgt cgcgttgggt ctcgtgccat tggctcgcgg
     1381 caatgtcgaa agtccgggaa ggggcgtagc gctggctcac cagcaggcgc ggggtgagca
     1441 ccagcgcaag cggggtggga cgcttcttgt cgtcctcggc gtcggcgaag ctcgacacct
     1501 gcaggaacag cgcatcaccc aggcaacggt tgcgtccggc gaggccgatg ccgcggatgt
     1561 cctcgcgctt cggcaaccgc acgcccgccg cgtcctggca ggcctcggtt tccccgccct
     1621 ccggctggca caggtcgagc catgccgcct ctgcggggat ggggtttccc gcttgccacg
     1681 tcgttgattg cgctttgccg tcgcaatgga tcgtcagcat gggaagcccg ggctggaatg
     1741 gaccgggcaa ggatagccgc gtccgccgcc ggttcggcat gtcgtcccgt gacgtgcgag
     1801 tcccgcatac gcccgcgacg tcagcggttg tcaccgctcg gagtccgggg ttgcctagca
     1861 catccggtcc cctgtcgcaa gtacatggtt cacgctttcg gacatccgtt gtgcactgca
     1921 gcgtgcgcgg cgtatttgcc gctgctagcg tgcgggcgcc gcgacccggg atctcgactg
     1981 cacgagccgc gaccaagggt ggttcgcgcg tgcgaaccga tcgagtctgg ggatcccgct
     2041 tcacggtcgc aatcatggtg ctgcacgtat cgccagatat gtgtcgggta gccgttgatt
     2101 cggtcgctgc tgcgtcgtgt aaccagacat tccttcatat cagtcaaggg agtctcggat
     2161 gcacaccaaa cttcatttga gtgccctggt ggtcgcgctc ggcatggcca tggccggggc
     2221 ggctggagcg gctgcgccag cgcccggcac ggcgatggca cacccgaccc ggttggcgca
     2281 gggcgacgtc gtcgccggac cgctcgcatt gtcgcaaccg atgcacatcg tcatcggcct
     2341 caagctgcgg aacaaggcgg agctggacgc gtacatcgcg aagccagggt tcaagccgct
     2401 ttccagtgcg cagttcgcgc agcgttattc gccgaccgcg gcgcaagccc agaaggtggc
     2461 cgatttcctg cggcagcgcg ggttcacgaa catcaggatc gcgccgaacc ggctgctggt
     2521 ttccggcgat gcgccggcgt cgagcgtgca aagcgcattc gacacgtcgc tggtcagcgt
     2581 ccggacgcat gatggccgcc aggcctacgc caacacgcgg gcggcaacgg tgccttcctc
     2641 gcttgccggg atgatcaacg gcgtgatggg cctgcagacc gtgcacatcc cgcacttgct
     2701 gcaccagaag accgggggca gggcaggcac caatgcagtc accggccaca gcccaacgga
     2761 gtttccttcg atctacggtg caacgagcct gccgaccgcg gccggcgtca cggtcggcat
     2821 ccttgcggaa ggcaagctca cccagaccat caactcgttg aatacattca cctcgcagaa
     2881 cggctatccg acggtgacga ctcggatcgt cggcacctcc acccgcgaca cggccggtac
     2941 cctcgagtgg gaactcgaca gccagaccat cgtgggtgcg gcaggcggcg aggtcggctc
     3001 gatcgtgttc tacatgatgc ccaacatcag caccgatacg gccgtagccg agggtatcaa
     3061 cctcgcggtc accgacaaca ccgcccaggt cgtcaatggg tcgctgggtg tcagcgaagt
     3121 ggacgcacag aacgacggga ccttcgcttc ctccgacgcc ctcctggaac aaggcgtcgc
     3181 gcaaggacag acattctcgt tctcgaccgg cgatgatggc gccagcagtt gctttgcgtt
     3241 gcaccaggac gcagcgtgct ggccggccac gtcgcaatac gtggtggctg ttggcggcac
     3301 gacactggac gccagcacgg ggccggacgc cacgtggaac caggaagtcg tctggggcgg
     3361 cgcgggcggc gccgagagcg aggtggagcc caagccgagt tggcagacgc tctggagcgg
     3421 cgatcaccgc gcggcgccgg acgtctcgtt cgacgcgagc ccgaactcgg gcgcgttgat
     3481 catcgacggc ggcggcactg tgcaggtcgg aggaaccagc ctttcctcac cgatgttcgc
     3541 gggcttctgg gcacgcgtcg tcgccggcaa gggcaacctc ggctttgccg caccgctgct
     3601 gtatccgctg tcggcctcgg acttccatga catcacccag ggcagcaacg gcaacgcggc
     3661 ggcaccgggt ttcgaccttg ccagcggccg cggcagcatg gtctggagcg aggtgtacca
     3721 ggaactgagc ggtggcggtg gcgacgagcc gccggtcgcg agcttcaccg tcaagacccg
     3781 cggaaagttc gtgcggctga cggacacgtc caccgatccc gacggaacca tcgcgtcacg
     3841 gatgtgggac ttgggggatg gtcgtacgtc aacgcgccag aagctcacgt atcgctatgc
     3901 gtcgccaggt acctacaccg tgaccctgac ggtgacggac aacgatggcg agtcgtcgtc
     3961 gacgagtcag gatgtaacca tcggtggcgg ccagtaatac acgcacgcag cgcgttttgc
     4021 ggaccgagcg gccgggtcat accggccgct attttcatgg aatgcggtcg gagcgccagc
     4081 ggctacgatg gcgttggcgt gtccggccaa ttgcgaggcg ccaatctgga cgattcttcg
     4141 gcgcgtctga tctgagaggg cgctgggagc ggacctgggc cgcctctggc gtcccaacca
     4201 cgcaaatctc ctggagtcag aattttacac aataggcatt tgcatcaatg tgaggtttgg
     4261 cttggcccga agcactggct tatagtcggg gctcccgcgc gcccccgcgc ggctcgaaac
     4321 ccacacggaa cgagggttcg tacatgccca gccagtctcc ttccaaccgc ggcggccagg
     4381 atcggctggc gcgattggct gccgaacgcg tggtgatcga ggaggtctgg cccgaaatcg
     4441 atggcggccg gcatccgatc aagcgcgtgg tcggcgacga gctggaagtc tgggcggaca
     4501 ttttctgcga cggccatgaa cggctcgcgg cctgcatctg ctggcgcgag gttggtgccc
     4561 gcaagtggcg cgaagcgccg atgcagctgg tgaacaacga ccgctggcgc gggacgttca
     4621 ccgtggaccg gaacacccgc tacgtctaca cgatcgaggc gtggcgtgac cgcttcgcga
     4681 cctggcgcga ggacttcctg aagaagcgcg acgcgggcca ggacatcgcc ctggaactgc
     4741 tcgagggtcg cgaactggtg cacgcggcgg ccgagggagc accccgcagc ttcgggtcca
     4801 tggtcaagaa cctcgacacc gccgcggacg acgcgcaact ccaggccctg ctgctcgacg
     4861 acgagctgct agaacggatg cgccacgagg gcacccgcac caacgcaacg cgctaccgcc
     4921 acgaactgga agtcgtcgtc gaccgaccgg cggcgcggtt cggcgcgtgg tacgagctgt
     4981 tcccgcgctc ggcctccaac gatgcgcacc gccacggcaa cttcgacgat gtgatcgcgc
     5041 gcctgccgta cgtacgcgaa ctcggcttcg acgtgttgta cctgccgccg atccacccga
     5101 tcggcgttag ccatcgcaag ggccgcaaca acgcgctgga agccgcgcag gacgatcccg
     5161 gcagcccgtg ggcgatcggc tcggccggcg gcggccacac cgcgatccat ccggaactcg
     5221 ggacgctcga agacttccgg cggctgatcg cggccgcgaa gaaacaaggc atcgagatcg
     5281 cgctcgactt cgcgatccaa tgctcacgcg accatccgtg gatcaaggaa caccccgaat
     5341 ggttcgactg gcgtccggac ggcacgatca agtacgccga gaacccgccc aagaaatacc
     5401 aggacatcgt caatgtccac ttctatcgcg acgccctgcc ctcgctgtgg ctggcgttgc
     5461 gggacgtggt gcggttctgg atcgaccagg gcgtgcgcat cttccgcgtt gacaatccgc
     5521 acaccaagcc cgtgccattc tgggaatggc tg
//
LOCUS       DASSNH010000226        21817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1144775, whole genome shotgun sequence.
ACCESSION   DASSNH010000226 DASSNH010000000
VERSION     DASSNH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 21817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21817
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144775"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..423)
                     /locus_tag="VFK00_08140"
     CDS             complement(22..423)
                     /locus_tag="VFK00_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HET7592955.1"
                     /translation="MAPATDIYYIADSRKPFEQAVADLDAAVRRHQFGVLHVHDLGAT
                     LRGKGQPSEGECKVFEVCNPRQAANVLARDLDLNMALPCRISVYTEGGNTRIGMIEPA
                     RMLGMLSDDAALGPIAREVGDATRRMIDEAK"
     gene            complement(444..650)
                     /locus_tag="VFK00_08145"
     CDS             complement(444..650)
                     /locus_tag="VFK00_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET7592956.1"
                     /translation="MNIDRMVMRFAGGMVIASVALAYFISPWWMLLALFVGLNLFQAS
                     FTGFCPLARILKKLGLQPGCAFPH"
     gene            846..1160
                     /locus_tag="VFK00_08150"
     CDS             846..1160
                     /locus_tag="VFK00_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7592957.1"
                     /translation="MASHSEEAARLLRALANPQRLRILCMLVGQELTVGRINENLPDL
                     SQSALSQHLAKLRDEDLVATRRDAQNVYYSLPPGPAQEIIAVLYGIYCGPSRPDRRRA
                     AR"
     gene            complement(1218..1781)
                     /locus_tag="VFK00_08155"
     CDS             complement(1218..1781)
                     /locus_tag="VFK00_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HET7592958.1"
                     /translation="MNLQTTRNRYNALSIAVHWLTLALLVAVYALILLRELYPKGSDP
                     RESMKAWHFILGLTVFGIVGVRMLLRLAFRTPPISPAPPAWQHALATAMPLALYAFLI
                     VMPLLGWLTLSAKGNPIPFFGLQLPALIGPDKALGGKLEEIHETIGTVGYYLVGLHAA
                     AALFHHYLMRDDTLRRMLPRRRRRELA"
     gene            complement(1810..3096)
                     /locus_tag="VFK00_08160"
     CDS             complement(1810..3096)
                     /locus_tag="VFK00_08160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HET7592959.1"
                     /translation="MAKIVVMGAGLGGMSAAFEIRAALPAEHGVTVVGDGPYFSFIPS
                     NPWVAIGKRTASQIRFEVAAPLRRHDIDFVDRKVVRVHPHERRLELADGSDLGYDYLV
                     ITTGPRLAFDEVPGLGPEGHTQSVCTTPHAERAWQAWQAFLENPGAIVVAAAAGASCF
                     GPAYETAFILDADLRRRKLRDRVPMTYVTSEPYIGHMGLGGVGDSQGLLEAELRKRHI
                     RWVTNARIRDVQAGQMHVEEVGAHGRIAQELAIPFSYAVVIPAFTGVDALRGIEGLVN
                     PRGFVLIDKHQRNPAFPEIFSAGVCVAIPPVEQTPVPTGAPKTGFMIESMVRAIACNL
                     RDELAGVPAQAEATWNAVCLADMGDTGAAFVALPQIPPRNVTWTRMGRWVHAAKLAFE
                     KYYLHKLRNGRPEPFYEKRILKWLGIEMLKPDVKPH"
     gene            complement(3385..3780)
                     /locus_tag="VFK00_08165"
     CDS             complement(3385..3780)
                     /locus_tag="VFK00_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592960.1"
                     /translation="MWLAGKAIVVWLVILGLAFANAALREAVLVPWIGKVRGLTLSGL
                     ILSALVLAVAYLALPWIGAVRIVELVAIGIAWLVFTLSFDLGMGAFQGRPVRRQFDAY
                     LFKRGNLWPVVLLVTAGAPLFAARLRGLI"
     gene            complement(3850..5247)
                     /gene="hemN"
                     /locus_tag="VFK00_08170"
     CDS             complement(3850..5247)
                     /gene="hemN"
                     /locus_tag="VFK00_08170"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET7592961.1"
                     /translation="MDTTAQFDPVLMARYDVSAPRYTSYPTAPQFHAGFGETQLCDAI
                     LASNNAAPLRPLSLYVHVPFCWSPCFYCGCTRVITRDPRNVDKYLDYLHRETAMIARS
                     FDARRPVRQLHFGGGTPNFLDFPRLETLMRDLAAHFRFAKGDDIEAGIEVDPRFADFD
                     YVCELGTLGFNRISIGVQDFDPAVQAAVNRIQTLAQTAAVVNGARAADFRSVNIDLIY
                     GLPKQTLDGFAQTLDQTIGLGPDRVAVYGYAHLPRLFKAQGQIESADLPGPAARLKLL
                     GLALEKLLGAGYVYIGMDHFARPDDELVRAQAQGTLQRNFQGYSTGGECDIVGLGMSA
                     IGRIGNCYSQNARDLPGYYMALDNGRLPVARGIMLDDDDVIRRAAIGNLMCHGFLDMQ
                     SFGSRHGIVFVDYFATELERMQQLEADALVELDSRMLRITPRGRFLMRNVAMCFDAYL
                     PAEMQAPQPRYAQSI"
     gene            complement(5316..5441)
                     /gene="cydX"
                     /locus_tag="VFK00_08175"
     CDS             complement(5316..5441)
                     /gene="cydX"
                     /locus_tag="VFK00_08175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HET7592962.1"
                     /translation="MWYFAWLLGTGLACTFGILNAMWCEVHADDERDWAAVINDY"
     gene            complement(5470..7017)
                     /locus_tag="VFK00_08180"
     CDS             complement(5470..7017)
                     /locus_tag="VFK00_08180"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7592963.1"
                     /translation="MSGPLGFHVSALWPHLVALYCMTLLPLAVGLAPIIAIIESVYVM
                     TGREIWKQAARFWGKLFGIILLTWAMGSIVLAILYILDSAPLTQYVHGTPSPALIAFF
                     LPFLLAFAWLLWSWFRDWWNPHRVRHLLVTWLWVPLFGIAVFKVAVGYGLLDNPAGVE
                     LDPGSLQVWIYDVPALLLNPAAQSRFVHLTGACYLTAATWVLSVSAWYLLRDRNVRIA
                     RRSLTVAASFGLAAALSLAVLGDRSGYGASPGQQMRIAAIAAEWNTQAAPAPFTIFGF
                     PDSGHRTTRMSVNLPWALGWGATHSWRKAVPGLDELESVNESRIRDGISAFTTLDTPW
                     ANPSGLSGPVFDSEDAPNFGYALLLLRSTSRPAYANDDQVAAAARSTIPDVPLLFWSF
                     RFMALLGVYSIVLFGWAFVLASRRRLEKRAFLRVALWSLPLPWIAGALGWIVSEAGRG
                     PWLLDGLLPVTETYASRSAVVIGSVACLGIAVIYAIGMVLLVRLVRRGPEELKFWPAE
                     SAQARKY"
     gene            complement(7062..7499)
                     /locus_tag="VFK00_08185"
     CDS             complement(7062..7499)
                     /locus_tag="VFK00_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7592964.1"
                     /translation="MFTHILLPIDDDPGSRHAIEIGVALAKGIGARITGFHAMTEFNH
                     AGIVEELLEPPPQELERLAQAHADKLLAPLRRKAERAGVRCETLARRGDRPHEAIVDA
                     AAARGCDLIVMASHGRRGIAKLVLGSQTQQVLNHTGISVLVVR"
     gene            complement(7563..8216)
                     /locus_tag="VFK00_08190"
     CDS             complement(7563..8216)
                     /locus_tag="VFK00_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7592965.1"
                     /translation="MKSDVELQHDVTAELDWEPSVDPSRISVDVRDGVARLAGLVGSY
                     AQKCDAEAAALRVRGVKGVIVEIDVELPESSRRDDVDIGEAAHHALSWNAAIPRGSVR
                     VAVRNGWVVLSGAVEWEYQSRAAAASVRNLFGVRGLTNLIEIKPRIEPRDVSRQVEAA
                     LRRRANHQARNLSVAVNGRTVTLSGQLDSWGERQAARLAAWNAPGIRNVVDQTTIAA"
     gene            complement(8280..8771)
                     /locus_tag="VFK00_08195"
     CDS             complement(8280..8771)
                     /locus_tag="VFK00_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET7592966.1"
                     /translation="MTQQQIRNLSALMDSRFTREIDEIRAVRARTRDEREEGAQGDRS
                     DAASFETTLATDDAVISQDVTDVRDILAARERLAAGTYGICTDCGDAIAYERLLAYPT
                     AKRCIHCQRVCEHGRSVGRILRVSRGGRPTTHVAARTGQNTESCAGSGSLDTTCAADL
                     RLS"
     gene            9178..9903
                     /locus_tag="VFK00_08200"
     CDS             9178..9903
                     /locus_tag="VFK00_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7592967.1"
                     /translation="MSTGMHLLVVDDDIGVLDLLRRYFTGQGFEVSTAANGAEMRDAL
                     TRTSIDLVLLDLGLPGEDGFELMRRLRKTWNGALIIITGRGESVDRVVGLELGADDYV
                     TKPFELRELLARVRSVLRRAAPAAKPDGQSDQAVFRFGDFLLDPGSRSLSTNRGETIA
                     LTTGEYELLRVLVERPNRVLSRDDLMECIHGRNAGPFDRAIDVQIARLRRKIEVDPAS
                     PELIKSVRGAGYLFSARVARQAR"
     gene            9904..11955
                     /locus_tag="VFK00_08205"
     CDS             9904..11955
                     /locus_tag="VFK00_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7592968.1"
                     /translation="MNPRAIPAASFVMLSTWFDIAPDAMIAVDQDGIIVLANTQAERM
                     FGYEHGALQGLTLEALVPESLRHGHRMHRHEYMHNPRVRPMGIGYELLGVKLDGQTFP
                     LEVGLSSIRIENGVVAVASVRDISETQRVRQALARARRDEYVASISRLALESPDYELA
                     VRRILELTTVALELPAAAIFTTDWRHSSLRLRASTGLSEQTARTVAAILGNAESVRST
                     LEAREHTVITSEMLREGPSEAIRADFSRAGFHDVALVPLYGRQEALGFLLVLASAPRS
                     FDKGKAAFLQSIASLLTSAIHRSRIEEQLAHSQRLDAIGQLTGGVAHDFNNLLTVISG
                     NLQLLEAELSDRPALQGTIDSALRAVDMGSNLTRRLLTFARRQPLQPRAVVPQPLLEE
                     IGAMLRRTLGESIHVQIDCAAAVPDVYADPNELDTALVNLALNARDAMPHGGRLTISA
                     REIGVDGAVNKWKLPSGHYVAITVGDTGTGMPPDVQAHAFEPFFTTKDSGKGSGLGLS
                     MVYGFATQSGGTITIDSRLGYGTRVELMLPAAASTAETSNDVQGDQAAPAKAHETILV
                     VEDENDVRTVATRFLTAVGYHVVAAASAREALDLLIANPDVDLLFSDVVLGSGMDGRE
                     LAHEARRIRPELAILLVSGYEDRAGRPHDDTPAEYFELLRKPYRREQLVTTIRRILDS
                     A"
     gene            complement(11962..12684)
                     /locus_tag="VFK00_08210"
     CDS             complement(11962..12684)
                     /locus_tag="VFK00_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7592969.1"
                     /translation="MDTPFVPAATETANPQQPTFEQPLELDLEELQRHMVVTRRKVKA
                     GQYLYRNGQPFHALYLVHAGFLKTCELSKDGREQVTGFRMRGDLLGVESIGLAVHTCD
                     AVALEDSQIWELPYPPVLSACLHIPELQVRLSAALAEEIRRDRSWMLTIGTLPAEQRV
                     AAFLLDLASRLARLGFSARHFILRMGRADIASFLALKHETVSRALSHLEQLEYISVLR
                     REVKLLDIPKLSACAGMPLTVQ"
     gene            12869..13957
                     /locus_tag="VFK00_08215"
     CDS             12869..13957
                     /locus_tag="VFK00_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592970.1"
                     /translation="MIDLHQPSPFIELAAVEAWDAWFRWREQTDLRDVSIEDTWLRVS
                     SALASVEPRGETAMWQARFMDALASWRLLPDERLLTDAGTGRPMRYGGGIHAALNAAS
                     FVSFDRGNVSALDLAALSECAMLTVRALDNVALLATVAAPRLHIGLLGVADALALLGL
                     AYDSDAGRVQAASMANALAQGCLRGNMLLAITRGAVASDTPVAFARAQSRGTSADLLR
                     DIARHGLRHAQLTAIAPHPRLALLANDVADAVDPLRGEDHPNVIAVPGGQRRTIRSSG
                     YALNVLQGRGNGSGEEPDTLANLPWTAQIAMRAAVQPWIDEPIDYPLLTTSDLDDARR
                     LEAQKLAASHGLGDPAWRDSAELQAMWP"
     gene            complement(13967..16366)
                     /locus_tag="VFK00_08220"
     CDS             complement(13967..16366)
                     /locus_tag="VFK00_08220"
                     /inference="COORDINATES: protein motif:HMM:NF003621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7592971.1"
                     /translation="MKTNVERGAVCPQVLGDVDAWWRAANYLSVGQIYLRANPLLREP
                     LKLEHVKLTLVGHWGTTPGQNFVYAHLNRIIKQHDLDMIYVSGPGHGGPAVVANAYLD
                     GTYSEVYPEITADEAGLQKLFKQFSFPGGIGSHATPECPGSIHEGGELGYSLSHACGA
                     VLDNPDLIAVCVIGDGEAETGPLATSWHSNKFLNPVTDGTVLPILHLNGYKIANPTVL
                     ARIPAEELEQFLRGCGWNPHFVRGSDPGAMHKLMAKTLDGVVEQIARIRRDARGHGVS
                     TRPTWPMIVLDSPKGWTGPKYVDGKPIEGTFHAHQVPLSISANTPPQHLAELEAWMRS
                     YRPEELFDGNGRVCEELRAFAPHGDRRMSANPHANGGLLLRDLRMPDFRAHAVDVPAP
                     GAPGIGDTHVLGRFMREVIRANEDQRNFRIFGPDETVSNGLEAAFEVTNRQWEPPTVA
                     TDEFLAREGRVLEMLSEHQCEGWLEGYLLTGRHGIFNCYEAFIHIVDSMFNQHAKWLK
                     MTACIPWRRRIASLNYLLASHVWRQDHNGFTHQDPGFIDHVVNKKAEIVRVYLPPDAN
                     CLLSVMDHCLRSRHYVNVVVAGKHPAPQWLDMRAAVSHCSRGIGIWPWASNDEDGEPD
                     VVLACAGDVPTLETLAAASILRRHAPNLKVRVVNVVDLMKLQSHSLHPHGLNDQDFDQ
                     LFTNGKPVIFAYHGYPALIHRLTYRRRNHDNFHVHGYKEEGTITTPFDMTVLNDLDRW
                     HLVLDVLQRVPGLGAKTAYLKQKLLDKLIEHGHYIREEGMDLPEVRDWSWSSPDAGVL
                     R"
     gene            complement(16366..17460)
                     /gene="adhP"
                     /locus_tag="VFK00_08225"
     CDS             complement(16366..17460)
                     /gene="adhP"
                     /locus_tag="VFK00_08225"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET7592972.1"
                     /translation="MPQTMRAAVVRGFGQPLSIEEWPVPRPGYGEVLVRITASGVCHT
                     DLHAADGDWPVKPSLPFIPGHEGIGRVVAAGPGVHHLKENDVVGIAWLHDACGHCEHC
                     VAGWETLCETQHNSGYSVNGCFAEYALGNAAYVARLPGHSDPMAMAPILCAGVTTYKG
                     IKETGARPGQWIAISGIGGLGHLAVQYATAMGMRVVAIDVAEDKLALARTDGAEVVVD
                     ARLDDAAQRVIEATGGGAHAVLVTAVSNSAFGQALRMVRRKGTVSLVGLPPGTFETSI
                     FDVVLKRITVRGSIVGTRQDLAEAIAFAAAGKVRAHTKAVSLEDINAVFADLKAGRVT
                     GRVVLDLDRRSERTAGAAIPSDVRLTASVE"
     gene            17581..18543
                     /locus_tag="VFK00_08230"
     CDS             17581..18543
                     /locus_tag="VFK00_08230"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HET7592973.1"
                     /translation="MRTAALPADASVETRHSSFDVLLKAAAALGPIPTAVVHPCDPLS
                     LQAALEAARSGSIQPILVGPRSRILRAAESASLDVGGCHIEDTPHSHAAAARAVGLAR
                     AGDVAALVKGALHTDELMAEVVDRERGLRTERRVSHVFALDVPRYPKLLLLTDGALNI
                     APDLLDKRDIIQNAIDLAHAIGIALPQVAILSAVETINPRIASTIDAAALCKMRDRGQ
                     ITGGTLEGPLAFDNAVSVAAAAIKGIESPVAGHADILVAPDLMTGNMLAKQMVYLAGA
                     SSAGVVLGARVPIALTSRADAVRARVMSCALARLVAHHASTVLT"
     gene            18540..19715
                     /locus_tag="VFK00_08235"
     CDS             18540..19715
                     /locus_tag="VFK00_08235"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET7592974.1"
                     /translation="MSRGILALNIGSSSIKLALFDAATLEPLLRGKVTDVGHDPHATL
                     TLPGHSTVSERPAAADIGEAVRWLLAELRERHSEIRLAAVGHRVVHGGTRFDKPVSID
                     ENVLAELEALAVLAPAHQPAEIALIRNVQELLPDVPQIACFDTSFHRTQPKLSQWFAL
                     PRALSESGIVRVGFHGLSYEYIASVLPQTAGTRPYGKTVVAHLGHGASVCALRDLRSV
                     STSMGFTPLDGLMMATRSGAIDPGVLLHLQQQRGMSPQEVADLLGNRSGLLGVSGISD
                     DVRVLEASDDPCAREALEMFAERTAAAIAAQCVALEGIDALVFTGGIGEHADRTRKRI
                     GERLAWLGVALDDARNARHGARISPDDARIQVLVIPTDEERMVARATAALLESPSHH"
     gene            19984..20454
                     /locus_tag="VFK00_08240"
     CDS             19984..20454
                     /locus_tag="VFK00_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7592975.1"
                     /translation="MTIRALRKEDAELEREFIRNLSPESRWMRFLGQISEPSDSLIRK
                     LTNLDYQRDMAFIALSHQGGGTREVGVSRYSLAPDGQSCECAVTVADAWQGKGLGTVL
                     MRDLIDIARRRGIRSMFSVDASANERMRELARDLGFERNRDPGDPTRVIYRLVP"
     gene            complement(20576..>21817)
                     /locus_tag="VFK00_08245"
     CDS             complement(20576..>21817)
                     /locus_tag="VFK00_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET7592976.1"
                     /translation="GRGWRATQRTFTKGRYDTEQTRNIQVVGNKVYHAFPMKDKAGKW
                     QLWTATSNVDGSSWQARQQTTSGGIIPSFKVSGNKIYYIFPAPTAKKKIYDIVIASAN
                     LDGSGWHELRRIHGGAWWIIAQLTVDKGVIYFSYAKGDPDKSVHLWTGNVATDGSGFK
                     ESQRTFGVGNSIPAGVQVVGDKIQYAFSVMQQPIAVMRKTGLMGFAFWTAKSDLNGDH
                     WVQQRLIGGDGDDHLTGYKMLSVVGGKAYYDVNRLHYVKNEGGMVRQENGVLAYSGSN
                     VLSKGDSYGIGMSASGIVSGFVNAGEDYLYHGEAPEDTAGAMVEQKLAPGWHHVAVTY
                     DGDLLRLYVDGTLARETRYDKKPAANPFPLSIGDGFVGKIARLKLFDRALDSSEVASL
                     ASRHSLTSCGLVLQRRGAGMR"
BASE COUNT         3960 a         7240 c         6750 g         3867 t
ORIGIN      
        1 ggtggatggg gcgctgccgc atcacttcgc ctcgtcgatc atccggcggg tcgcgtcacc
       61 gacctcgcgc gcgatcggcc cgagcgcggc atcgtccgac agcatgccga gcatccgcgc
      121 cggttcgatc atgccgatgc gagtgttgcc gccttcggtg tacaccgaga tccggcacgg
      181 cagggccatg ttgaggtcga ggtcgcgggc cagcacgttg gcggcctggc gcggattgca
      241 cacctcgaac accttgcatt cgccctcgga gggctggccc ttgccgcgca acgttgcgcc
      301 gaggtcgtgc acgtgcagca cgccgaactg gtgccggcgc acggcggcgt cgaggtcggc
      361 cacggcctgc tcgaacggct tgcgggagtc cgcgatgtag tagatgtctg ttgctggtgc
      421 catgatgcaa accttccctg ctttcagtgg gggaatgcgc agcccggttg caggccgagc
      481 ttcttgagga tccgcgccag cgggcagaaa cccgtgaacg aggcctgaaa caggttcagg
      541 ccgacgaaca acgccagcag catccaccac ggcgaaatga aatacgccag cgccacgctc
      601 gcgatcacca tgccgccggc aaaacgcatc accatccgat cgatgttcat cgtcacgctc
      661 cgtcgatgtg ggtctttgca ctcctggaac cgctgcgggc gacgcatccg tcccgggctg
      721 gtcgtgtata tcttgttgtg ctaatgtagt atcatcttat ttagaatgac gtatactgtc
      781 aacacggccg ccgccggaca cctccgatga agcaagcccg cgcccctgcc ccgcgccttg
      841 gacaaatggc atcccattcc gaagaagccg cgcggctgct gcgggcgctc gccaatccgc
      901 aacgcctgcg catcctgtgc atgctggtcg gccaggagct gacggttggc cgcatcaacg
      961 agaacctgcc cgacctgagc cagtcggcgc tgtcccagca cctggcgaaa ctgcgtgacg
     1021 aggacctggt ggccacccgc cgcgatgccc agaacgtcta ctactcgctg ccgccgggac
     1081 cggcacagga aatcattgcg gtcctgtacg gcatctactg cggcccgtcc aggcccgacc
     1141 gccgtcgcgc cgcgcgctga cggcaacgaa gtgcgggtct ggcctgcgtg accgggcatg
     1201 caccgcacgt cccacgctca agccagctcg cggcgccggc ggcggggcaa catccgccgc
     1261 agcgtgtcat cgcgcatcag gtaatggtgg aacagcgccg ccgcggcgtg caggccgacc
     1321 aggtaatacc cgacagtgcc gatggtctcg tggatttcct cgagcttgcc gcccaatgcc
     1381 ttgtccggcc cgatcagggc cggcaactgc aacccgaaga acgggatcgg gttgcccttc
     1441 gcactcagcg tgagccagcc gagcaacggc atcacgatca ggaatgcgta cagcgcgagg
     1501 ggcatggccg tggccagcgc atgctgccac gccggcggcg ccggcgaaat gggcggtgtc
     1561 cggaatgcca ggcgcagcag catccgcacg ccgacgatgc cgaacacggt cagccccagg
     1621 atgaaatgcc aggccttcat cgactcgcgc ggatcgctgc ccttgggata cagctcgcgc
     1681 agcaggatca atgcatacac ggccaccagc agcgccagcg tcagccagtg caccgcgatc
     1741 gacaaggcgt tgtaacgatt gcgggtggtt tgcaggttca tgaaagtcct ccgtcgcggc
     1801 gtgcagccct cagtgcggct tgacatctgg tttcagcatt tcgatgccca accatttcag
     1861 gatgcgcttc tcgtagaacg gttcgggtcg cccgttgcgc aacttgtgca ggtagtactt
     1921 ctcgaacgcc agcttggccg cgtgcaccca tcgccccatg cgcgtccagg tgacattgcg
     1981 cggcgggatt tgcggcaggg ccacgaacgc cgcgccggtg tcgcccatgt cggccaggca
     2041 cacggcgttc caggtggcct cggcctgcgc gggcacgccg gccaattcgt cacgcaggtt
     2101 gcaggcgatc gcgcgcacca tcgattcgat catgaagccg gtcttgggcg ccccggtcgg
     2161 caccggggtc tgctcgaccg gaggaatcgc cacgcacacg ccagcggaga agatttcggg
     2221 aaaggccggg ttgcgctggt gcttgtcgat cagcacgaac ccgcgcggat tgaccagccc
     2281 ttcgatgccg cgcagcgcgt cgacgccggt gaacgcggga atcaccacag catacgaaaa
     2341 cgggatcgcg agctcctggg cgatgcggcc gtgcgcaccc acttcctcga cgtgcatctg
     2401 gccggcctgc acgtcccgga tgcgcgcgtt ggtgacccat cggatgtggc gcttgcgcag
     2461 ctccgcttcc agcaacccct gcgaatctcc gacgccgcca agccccatgt gtccaatgta
     2521 gggttcgctg gtcacgtaag tcatgggcac ccggtcgcgc agcttgcgcc ggcgcagatc
     2581 ggcgtcgagg atgaaggcgg tttcgtaggc cgggccgaag cagctcgcgc ccgctgccgc
     2641 ggccaccacg atggcccctg gattttccag gaacgcctgc caggcttgcc aggcacgttc
     2701 ggcgtggggc gtggtgcata ccgactgggt gtgaccctcc gggccgaggc ccggcacttc
     2761 gtcgaacgct agccgcggcc cggtggtgat caccaggtag tcgtagccga ggtcggagcc
     2821 gtcggccagt tccaggcgac gctcgtgcgg atgcacgcgt acgaccttcc ggtcgacgaa
     2881 atcgatgtcg tgccggcgca acggtgcggc gacttcgaac cggatctggc tcgcggtgcg
     2941 cttgccgatg gcgacccaag gattggaggg tatgaacgag aaatacggcc catcgcccac
     3001 caccgtcacg ccgtgttcgg cgggcagcgc ggccctgatc tcgaatgcgg cgctcatgcc
     3061 gccgagacca gcacccatca ccacgatctt cgccatgatc atccctccga agggtttgct
     3121 agaaactaac ccccgccact ccgggcgagt tgacgacggt caactcaagc cgcgatcggc
     3181 gttgcatcgt ccaaagaatg ctcagtggac ggggcatgcg cctcgcggcg cattcgccct
     3241 cgacgcgacc gcaatcccgc cccggaccac acggaacatt gcttgcacca cagccgtgcc
     3301 agctcacgac tccggcgagt ctcccgtgcc gccctgacgc cgcgcatatc ttgcggtcag
     3361 tcggcagatc tgccctgcac ccactcagat caggcccctg agtcttgctg cgaacaacgg
     3421 cgcgccggcg gtgaccagca ggacgacggg ccagagattg ccccgcttga agagataggc
     3481 gtcgaactgt cggcggactg gcctgccctg gaacgcaccc atccccaggt cgaaggacaa
     3541 ggtgaatacg agccacgcaa ttccgatggc gaccaactcg acgatgcgca cggcgccaat
     3601 ccacggcagc gccagatagg cgaccgccag caccagcgcc gacaggatga ggccgctcaa
     3661 cgtcagtccg cgcaccttgc cgatccacgg caccagcacc gcttcgcgca gcgccgcatt
     3721 ggcgaacgcc agccccagga tgaccagcca taccacgatt gcctttcctg ccagccacat
     3781 caccacggga tcacctaggg acttcgcggc tcgggttttc cgccatggcg gccggtagcc
     3841 ggctcgcggt cagatgcttt gcgcgtagcg aggttgcgga gcctgcattt ccgctggaag
     3901 gtaggcatcg aagcacatcg caacgttgcg catcaggaag cgcccgcgcg gcgtaatgcg
     3961 cagcattcgt gaatccagct ccaccagggc atccgcttcc agttgctgca tgcgctccag
     4021 ttcggtcgcg aaatagtcga cgaacacgat gccgtggcgc gatccgaagg actgcatgtc
     4081 caagaatccg tggcacatga gattgccgat cgccgcgcgt cggatcacgt cgtcgtcatc
     4141 gagcatgatt ccccgcgcca cgggcaggcg cccgttgtcc agcgccatgt aataacccgg
     4201 aagatcgcgc gcgttctggc tgtagcaatt gccgatgcgg ccgatcgcgc tcatgcccag
     4261 ccccacgatg tcgcattcgc caccggtcga atacccctgg aaattgcgtt gcagcgtgcc
     4321 ctgcgcttgc gcgcgcacca attcatcgtc gggacgcgcg aagtgatcca tgccaatgta
     4381 cacataaccc gcacccagca gtttttccaa cgcaagccca agcaacttca gccgggctgc
     4441 ggggccgggc aagtcggcgg actctatctg cccctgtgcc ttgaacaggc gcggcaggtg
     4501 cgcgtagcca tatacggcca cccggtcggg gccgaggcca atggtctggt cgagcgtctg
     4561 cgcgaaaccg tccagcgtct gcttcggcag accgtagatc aaatcgatgt tgacggaccg
     4621 aaagtcggcg gcacgcgcgc cgttcacgac ggcggctgtt tgcgccagcg tctggatccg
     4681 gttgactgct gcctgcacgg ccgggtcgaa atcctgcacg ccgatcgaga tgcggttgaa
     4741 gccgagcgtg ccgagttcgc agacgtaatc gaaatcggcg aagcgcgggt ccacttcgat
     4801 accggcctcg atatcgtcac ctttcgcaaa acggaaatgt gcggcaaggt cgcgcatcag
     4861 cgtctcgagg cgcgggaaat ccaggaaatt cggcgtgccg ccgccgaaat gcaattgccg
     4921 caccggccgc cgggcatcga acgaccgcgc gatcatcgcc gtctcgcgat gcaggtagtc
     4981 gagatacttg tcgacgttgc gcgggtcgcg cgtgatcacc cgcgtgcagc cgcaatagaa
     5041 gcaggggctc cagcaaaacg gcacgtggac gtacagcgac aatggccgca gcggggccgc
     5101 gttgttcgag gcgagaatcg cgtcgcacag ctgcgtctcg ccgaaacctg cgtggaattg
     5161 cggtgccgtc gggtaactgg tatagcgcgg tgcggacacg tcgtaacgcg ccatcagcac
     5221 ggggtcgaat tgggcggtcg tatccattgc cgtagtctcc gcgaccaccg ggcttggtcg
     5281 tttgaccgag gtcaatccag tgcaggaatt ggcactcagt agtcgttgat gactgccgcc
     5341 cagtcccgtt cgtcgtccgc atgcacctcg caccacatcg cgttcaggat gccgaaggtg
     5401 catgccaggc cagtgcccag cagccatgcg aagtaccaca tcgtgcgacc tctcgattcg
     5461 atcggatgac tagtatttcc tcgcctgcgc ggattccgcc ggccagaact tcaattcttc
     5521 cgggccgcgc cggaccaggc gcaccagcaa gaccatgcct atcgcgtaaa tgactgcaat
     5581 gcccagacac gcgacggagc caatgaccac ggcggaccgg ctggcgtacg tctcggtgac
     5641 gggtaacagc ccgtccagca gccacggtcc gcgcccggct tcactcacga tccagcccag
     5701 cgcccccgcg atccatggca gtggaaggct ccacaaggcg accctcagga acgcccgttt
     5761 ctccagccgg cgcctcgacg ccagcacgaa tgcccaaccg aacagcacga tgctgtatac
     5821 gcccaacagt gccatgaacc ggaagctcca gaacagaagc ggcacgtccg gaatcgtact
     5881 ccgggccgcg gcggcaactt gatcgtcgtt tgcgtacgcc gggcgcgagg tgctacgcaa
     5941 cagcaacaac gcgtagccga aattcggtgc atcttccgaa tcgaagacag ggccgctcag
     6001 gcccgaagga ttcgcccatg gcgtatcgag cgtggtgaat gccgatatgc catcccggat
     6061 gcgcgactcg ttcaccgatt cgagttcgtc caggccgggg accgcttttc tccacgaatg
     6121 ggtcgcaccc caccccagcg cccaaggaag gttcaccgac atgcgtgtcg ttcgatggcc
     6181 actgtccgga aaaccgaaga tcgtgaacgg tgctggtgcg gcttgggtat tccattccgc
     6241 cgcaatcgcg gctatccgca tctgttggcc aggggacgcg ccatagccgc tccggtcgcc
     6301 gagcaccgcc agcgagagcg ctgccgccaa accgaagctc gccgccacgg tcaacgaacg
     6361 ccgggcgatc cgcacattgc ggtcgcgcaa caggtaccac gccgaaaccg aaagcaccca
     6421 agtcgccgcg gtgagatagc acgcacccgt gagatgcacg aaccgcgact gcgcggccgg
     6481 attcaggagg agcgccggca cgtcgtaaat ccagacctgc aggctgccgg gatcaagctc
     6541 gacgcccgcg gggttgtcca gcaatccgta cccgacggcg accttgaaca ccgcaattcc
     6601 gaacagcggg acccacagcc aggtaacgag cagatgccgc acgcgatgcg gattccacca
     6661 atcgcgaaac cagctccaca gcagccaggc aaaagccagc aagaacggca ggaaaaacgc
     6721 gatcagcgca gggctgggtg ttccatggac atactgggtg agcggggcgg aatccaggat
     6781 gtacaggatc gcgagcacga tggagcccat ggcccacgtc agcaggatga tgccgaacag
     6841 cttgccccag aagcgggcgg cctgtttcca gatttcccgc ccggtcatga cgtaaacgct
     6901 ttcgatgatc gcaatgatcg gcgccagtcc taccgccagc ggcagcaacg tcatgcagta
     6961 cagcgccaca aggtgcggcc atagtgctga aacatggaac cccaaggggc cgctcatggc
     7021 gtttgctcag gaatcttggc cgaccggcgg tgcaaggcga ctcagcgcac caccagcacc
     7081 gaaatgccgg tatggttcag gacttgctgc gtctggctgc cgagcacgag cttcgcaata
     7141 ccacgccggc cgtgggacgc catgacaatc aggtcgcagc cccgcgccgc tgctgcatcg
     7201 acgatcgcct cgtggggccg atcgccgcgc cgcgccagcg tttcgcaccg cacccccgcg
     7261 cgctcggctt tgcgccggag cggggcgagc agtttgtccg catgcgcctg tgccaaccgt
     7321 tccaattcct gcggcggcgg ttccagcaat tcctccacga tgccggcgtg attgaattcc
     7381 gtcatcgcgt gaaagcccgt gatgcgcgcg ccgataccct ttgccagagc cacgccgatc
     7441 tcgatcgcgt gcctggagcc ggggtcgtca tcgatcggaa ggaggatatg cgtgaacatg
     7501 tcggagattc ccctggaccg cgtaccttcc ctggtgcgcg gcccgtacgc gttggccgcg
     7561 gctcatgccg cgatcgttgt ctggtcgacc acattgcgga tgccgggcgc gttccatgcg
     7621 gcgaggcgtg ccgcctggcg ttcgccccag gagtccagtt gacccgaaag cgtcactgtc
     7681 cgcccattca ctgcgaccga aaggttcctg gcctgatgat tggcgcgccg gcgcaacgcc
     7741 gcctcgactt gtcgcgagac gtcgcgaggt tcgatcctcg gcttgatttc gatgaggttg
     7801 gtcaggccgc gcacgccgaa caggttgcgc accgaagccg cggcggcgcg gctctggtac
     7861 tcccattcga cggctcccga caacacgacc cacccgttcc ggaccgccac cctcacgctc
     7921 ccgcgcggga tcgcagcatt ccaggacagt gcatggtggg ccgcctctcc gatatcgacg
     7981 tcgtcccgcc gactgctctc cggcaattcg acatcgatct ccacgatcac gcccttgacg
     8041 ccgcgtaccc gcaaggcggc ggcctccgca tcgcactttt gcgcatagct gcccaccagc
     8101 ccggccagtc tcgcaacacc gtcgcggaca tccacactga tccgtgacgg atcgacggac
     8161 ggttcccaat ccagttccgc cgtcacgtca tgctggagtt ccacgtcgga cttcatttcc
     8221 gcatctcctc acccgcaggg cgaatatcga cctttccggc ccaacagtct tgatgcgcat
     8281 caactcaatc gaagatcggc tgcgcaggtg gtgtcgagac tcccactccc cgcgcagctt
     8341 tcggtgttct gccctgtgcg cgcagctacg tgcgtggtag gtcgcccgcc cctcgacacg
     8401 cgcaggatcc ggccaacgga tctcccgtgc tcgcacaccc gctggcaatg aatgcagcgc
     8461 ttggccgtgg ggtaggccag caggcgttcg tacgcaatcg cgtcgccgca atccgtgcag
     8521 atgccgtacg tacccgccgc caagcgctcg cgcgcagcca ggatgtcccg cacgtctgtg
     8581 acatcctggc tgatcaccgc atcatccgtc gcgagcgtgg tttcgaacga cgccgcgtca
     8641 ctccggtcac cctgagcccc ttcctcgcgc tcgtcccgcg tgcgtgccct gacagcgcgg
     8701 atctcatcga tttcgcgggt gaaccgcgag tccatcaggg cggacagatt tctgatttgt
     8761 tgctgcgtca atttggtcat attgcctcca gactcatcgc ggtctcgtcg attaccgttg
     8821 tcgcgactca atgcttcaaa cgaacagcgg aaacgacccg cttccagccc cgctcgaatt
     8881 ggattgccat cgccatttct tcccgcattg cagggctccg ctgcgccaat gacaacagca
     8941 tgggtccttc atgggcgatg aaggtgacgt ggatcaagcc gcacgcactt cagcggttgg
     9001 cagccgggcg ggacagacga tgccacgggt gcgggagcga acgaagctgt gtcgccccat
     9061 ccggcgggtc ctctcctccc gcgcaggtgg acaggcgacc ctccgcgcgg atcccaaagc
     9121 cctgccacaa cggcggtgcc ggtatagaat ctggcgcatt ccgtcgggca cagtgccatg
     9181 tcgactggta tgcatttgct ggtcgtggac gacgatatcg gcgtcctgga cctgcttcgc
     9241 cgctatttca ccggccaagg cttcgaggtc agcaccgcgg cgaacggcgc cgagatgcgt
     9301 gacgccctca cacgcacgag catcgatctg gtattgctcg acctcggcct ccccggcgaa
     9361 gatggcttcg agctgatgcg ccggctccgc aaaacctgga atggcgccct catcatcatc
     9421 accggccgcg gcgaatcggt cgatcgcgtg gtcgggctgg aactgggtgc ggacgattac
     9481 gtcaccaaac ccttcgaatt gcgcgagttg ctggcgcgcg tgcgcagcgt actgaggcgc
     9541 gccgctccgg ccgccaagcc cgatgggcaa tccgaccagg ccgtgtttcg tttcggcgat
     9601 ttcctcctcg accctggctc gcgcagcctc tccaccaatc gcggtgagac gatcgcactg
     9661 acaaccggtg aatacgagtt gttgcgcgtg ctcgtcgaac gccccaaccg cgtactgtcg
     9721 cgcgacgacc tgatggaatg catccatggc cgcaacgccg gcccgttcga ccgcgccatc
     9781 gatgtgcaga ttgcacgact gcgtcgcaaa atcgaggtgg atccggcgag ccccgagctg
     9841 atcaaatcgg tgcgcggcgc cgggtatctc ttttcagcgc gtgtcgccag acaggcacga
     9901 tgaatgaatc cgcgggcgat tcctgccgcc agtttcgtga tgttgagcac ctggttcgac
     9961 atcgcgcccg acgcgatgat cgcggtcgat caggatggca tcatcgtgct cgccaataca
    10021 caggccgagc ggatgttcgg ctacgagcac ggcgcgctgc agggtttgac tctggaggct
    10081 ctggttccgg aatcgctgcg ccacgggcat cggatgcatc gacacgagta catgcacaac
    10141 ccgcgcgttc gaccgatggg catcggctac gagctgctgg gcgtgaagct ggacggccag
    10201 acattccccc tggaggttgg cttgagctcg atacgcatcg aaaacggcgt cgtcgccgtt
    10261 gcgtcggtgc gtgacatttc cgaaacgcaa cgtgtacgcc aggcgctcgc ccgggcccgc
    10321 cgcgacgaat atgtcgcatc catcagccgg ttggcgctgg aatcgcccga ttacgagctc
    10381 gcggtccgcc ggatactcga attgaccacg gtcgcgctgg agctgccggc ggcagccatt
    10441 ttcacgaccg attggcgcca cagctccctc cggctccgtg cctctaccgg gctttcggaa
    10501 cagaccgccc ggacggtcgc agccattctt ggcaacgcgg aatccgttcg cagcacgctg
    10561 gaagcacgag aacataccgt gatcacttct gaaatgctgc gggaggggcc ttcagaggct
    10621 attcgcgcgg acttttccag agcgggcttt cacgatgtcg cactggttcc gttgtacggg
    10681 cggcaggaag cgctgggctt cctgcttgtg ttggcgagcg caccgcggag cttcgacaaa
    10741 ggcaaggctg cctttttgca gtcgatagca agcttgctga cgtcggccat acatcgcagt
    10801 cgaatcgagg aacagctggc gcactcgcaa agactcgacg caatcggcca gcttaccggt
    10861 ggcgtcgccc atgacttcaa caacctgttg accgtgattt ctggaaacct gcagttgctg
    10921 gaggcggaat tgtccgaccg tccggccctg caagggacga tcgacagtgc cctgcgcgcc
    10981 gtggacatgg gatcgaatct cacccgccgg ctgctcacct tcgcgcgcag gcagccgctg
    11041 cagccgcggg cggtcgtgcc gcagcccttg ttggaagaaa tcggggccat gctgaggcgc
    11101 accctgggcg aatcgatcca cgtgcagatc gattgcgcgg cggcggtgcc cgacgtgtat
    11161 gccgatccca acgaactcga cacggccctg gtcaaccttg cactgaatgc gcgtgacgcc
    11221 atgccgcatg gcgggaggct gaccatcagc gcgcgggaaa tcggcgtgga tggcgccgtc
    11281 aacaaatgga agttgccgtc cggccactac gtggcgatta ccgtcggcga caccggcacg
    11341 ggcatgccac ccgacgtgca agcacatgca ttcgaaccct ttttcaccac caaggattcc
    11401 ggcaagggca gcgggctcgg tctcagtatg gtctacgggt tcgccaccca atccggcgga
    11461 accatcacca ttgacagccg gctgggctat ggaacgcgcg tggaactcat gttgcctgcg
    11521 gccgcctcaa ccgccgaaac cagcaatgac gtacagggcg accaggccgc cccggcgaaa
    11581 gcacatgaaa cgatattggt cgtcgaggac gaaaacgatg ttcgcaccgt cgccacgagg
    11641 ttcctgaccg ccgtcggcta ccacgtggtt gccgcggcca gtgcacgcga agcgctggat
    11701 ctccttatcg caaacccgga cgtcgacctg ctgttttcgg acgtggtgct ggggagcggc
    11761 atggatggca gggagctcgc gcacgaggca agacgcattc gtccggagct ggcgatactc
    11821 ctcgtttccg gctacgaaga ccgggccggc aggccacacg acgacacccc ggccgaatac
    11881 ttcgaattgc tgcggaaacc gtatcgacgc gagcaactcg tcacgacgat caggcggatc
    11941 ctcgacagcg cctgaattcc gtcactgcac cgtaaggggc atcccggcgc aggcgctcag
    12001 cttcgggatg tccagcagct tcacctcgcg acgcagcacc gaaatgtatt cgagctgttc
    12061 gaggtgcgac agcgcccggc tgacggtctc gtgcttcagc gcgaggaaac ttgcgatgtc
    12121 ggcacggccc atgcgcagga tgaagtggcg ggcgctgaac cccaggcggg ccagacgcga
    12181 agccaggtcc agcaggaacg ccgcaacccg ttgttcggcc ggcagtgtgc cgattgtcag
    12241 catccaggaa cggtcgcggc ggatttcctc ggcgagggcc gcggacaacc ggacctggag
    12301 ttcgggaatg tgcaaacacg cgctcaacac ggggggataa gggagttccc atatctgcga
    12361 atcctcgagt gcgacggcat cgcaggtgtg caccgccaaa ccgatggatt cgacccccag
    12421 caggtcgccg cgcatccgga aaccggttac ctgctcgcgg ccgtctttcg acaattcgca
    12481 ggtcttcagg aagcctgcgt gcacgaggta caacgcgtgg aacggctgcc cgttccggta
    12541 aagatattga cccgccttga ccttccttcg cgtcacgacc atgtgccgct gcaactcctc
    12601 gagatcgagt tcgagcggct gttcgaatgt tggctgctgc ggattcgccg tttcggtggc
    12661 ggccggcacg aaaggagtat ccatgacgct gctccggtgg tgggtgttcg acgccacgat
    12721 gtcgcatcaa cgtcaccgca cgatgtttcc aacgcaacgt tctgtaacgg tgtgtaacag
    12781 gagcggcccc ccggtgggtt cttgatcagc atcaagccgc cggccggagc cgcagcgata
    12841 gtgggcacag tccacgtggt gttgcttcgt gatcgatctg catcaaccat cgccgttcat
    12901 cgagctcgcc gccgtcgagg cctgggatgc gtggttccgg tggcgcgagc aaaccgatct
    12961 gcgtgacgtt tcgatcgagg atacgtggct gcgggtatcc agcgcattgg cttcggtcga
    13021 gccacgcggc gagaccgcaa tgtggcaggc gcgtttcatg gatgcattgg catcctggcg
    13081 gttgttgccc gacgagcgac tgctcacgga cgccggcacc ggtcggccga tgcgttacgg
    13141 cggcgggatc catgcagccc tgaacgcggc atccttcgtc tccttcgatc gcggcaatgt
    13201 gtcggcgctc gaccttgcgg ctctttcgga gtgcgcgatg ctgaccgtgc gcgcgctgga
    13261 caatgtggcg ttgctggcca cggtcgcggc ccctcgtctg cacatcgggt tgctcggtgt
    13321 cgccgacgcg ctggcgctgc tgggcctggc ctacgacagc gatgccggtc gcgtgcaggc
    13381 ggcatccatg gccaacgcct tggcgcaggg ttgtctgcgc ggcaacatgc tgctcgccat
    13441 cacgcgaggt gcagtcgcca gcgacacgcc cgttgccttc gcgagggccc aatcacgggg
    13501 tacttcagcg gatctgttgc gcgatatcgc ccgtcacggc ctgcgccatg cgcagctgac
    13561 cgccatcgct ccacacccac gactcgcatt gctcgccaat gacgtggcgg atgcagtgga
    13621 cccgctgcgg ggagaggacc acccgaacgt catcgccgtc ccgggtggac aacgacgcac
    13681 gatacgatcc tccggctacg cgctgaacgt gttgcaaggc cgcggaaacg gatccggaga
    13741 ggagccggac acactggcga atttgccttg gaccgcgcag atcgcgatgc gcgccgccgt
    13801 gcagccttgg atcgacgagc ccatcgatta tccgctgctg acaacgagtg acctggacga
    13861 tgcgcggcgg ctcgaagcgc aaaagcttgc cgcgagccac gggctgggcg atccggcctg
    13921 gcgcgacagc gccgaactgc aggcgatgtg gccgtaggtg ccatgctcag cggagcacgc
    13981 cggcatccgg tgacgaccac gaccaatcgc gcacctcggg caggtccatg ccctcttccc
    14041 ggatgtagtg accgtgctcg atcaacttgt cgagcagctt ttgcttgagg taggccgtct
    14101 tcgcgcccag tccgggcacg cgctggagca cgtccaacac gaggtgccag cggtcgagat
    14161 cattcagcac ggtcatgtcg aagggcgtgg tgatcgtgcc ctcttccttg tagccgtgca
    14221 cgtggaagtt gtcgtgattg cggcgccgat aggtcaggcg gtggatcaac gccggatagc
    14281 cgtggtaggc gaagattacc ggcttgccgt tggtgaacaa ctgatcgaag tcctggtcgt
    14341 tcaagccatg cggatgcagg ctgtgcgact gaagcttcat caggtccacc acgttgacca
    14401 cgcgcacctt caaattcggc gcatggcggc gcaggatcga tgccgccgct agcgtttcca
    14461 gcgtgggaac gtcgcccgcg caggccagca ccacgtcagg ctccccgtcc tcgtcgttgc
    14521 tcgcccacgg ccagatgcca atgccgcggc tgcaatggct gactgccgcc cgcatgtcca
    14581 gccactgcgg ggccgggtgc ttgcccgcga ccaccacgtt gacgtaatgc cggctgcgca
    14641 ggcagtggtc catcaccgac agcaggcaat tggcgtcggg cgggaggtac acgcgcacga
    14701 tctccgcctt cttgttgacc acgtggtcga tgaagccggg atcctggtgc gtgaaaccgt
    14761 tgtggtcctg ccgccatacg tgcgaggcca acaggtaatt gagcgaggcg atcctgcgcc
    14821 gccatggaat gcacgcggtc atcttcaacc acttggcgtg ctggttgaac atcgaatcca
    14881 cgatgtggat gaacgcttcg tagcaattga agatgccatg gcggcccgtc agcaggtagc
    14941 cctccagcca gccttcgcat tgatgctcgc tcagcatttc gagaacccgc ccctcgcggg
    15001 caaggaattc gtcggtcgca acggtgggcg gctcccattg ccggttggtg acctcgaaag
    15061 ccgcctccag gccgttcgac acggtttcat ccggcccgaa gatgcgaaaa ttgcgctggt
    15121 cctcgttggc gcggatcact tctcgcatga accggccgag cacgtgcgtg tcgccaatgc
    15181 cgggggctcc cggcgcgggc acgtccactg cgtgcgcacg gaaatccggc atgcgcaggt
    15241 cgcgcaacaa cagcccgccg ttggcgtgcg ggttggcact catgcgccga tcgccgtgcg
    15301 gtgcgaacgc gcgcagctcc tcgcacacgc gcccgtttcc gtcgaacagc tcctccggac
    15361 gatagctgcg catccatgcc tccagctcgg cgagatgctg cggaggcgtg tttgccgaga
    15421 tcgataacgg cacctgatgc gcgtggaagg tgccctcgat cggtttcccg tccacgtact
    15481 tgggcccggt ccagcccttc ggtgaatcca gcacgatcat cggccaggtc ggacgcgtcg
    15541 agacaccgtg cccgcgtgcg tcgcggcgga ttcgcgcgat ttgctcgacc accccatcga
    15601 gcgtcttggc catcagcttg tgcatcgctc caggatcgct cccacggacg aagtgcggat
    15661 tccagccgca accacgcagg aactgctcca gttcttccgc cggaatgcgc gccaacaccg
    15721 tcggattggc aatcttgtat ccgttgagat gcaggatcgg cagcaccgtc ccgtcggtga
    15781 ccgggttcag gaacttgttg gaatgccacg acgtcgccag cggaccggtt tcggcctcgc
    15841 catcgccgat cacgcagacg gcgatcaggt ccggattgtc gagcacggcg ccgcacgcat
    15901 ggctcaggga atagccgagt tcgccgcctt cgtggatgga accggggcac tccggcgtgg
    15961 cgtggctgcc gatgccgccc ggaaacgaaa actgcttgaa gagcttctgc aagccggctt
    16021 cgtctgcggt gatttccgga tagacctcgc tgtaggtgcc gtccaggtag gcattggcta
    16081 ccaccgccgg cccaccatgg cccggaccgg agacgtagat catgtccagg tcgtgctgtt
    16141 tgatgatccg gttgagatgg gcgtaaacga aattctgccc cggcgtggtg ccccaatggc
    16201 ccaccaaggt aagtttgacg tgttccagct tgagcggttc gcgcaacagc ggattcgcgc
    16261 gcagatagat ctggccgacc gaaaggtagt tcgctgcgcg ccaccaggca tccacgtcgc
    16321 caagaacttg cggacaaacc gccccgcgct cgacattggt tttcattatt cgacgctcgc
    16381 tgtcaaccgg acatcacttg ggatcgccgc acccgcggtc cgttcactcc gccgatcaag
    16441 atccaggacg acacgccccg ttacccggcc cgccttcagg tcggcgaaca cggcattgat
    16501 atcctcgagc gataccgcct tcgtgtgggc ccgcaccttg cccgccgcgg caaatgcgat
    16561 cgcctcggcc aggtcctggc gtgtgccaac tatggatccc cgaacggtga tccgcttcaa
    16621 cacgacatcg aagatcgacg tctcgaaggt tcccggcggc agtccgacca ggctcaccgt
    16681 acccttgcgg cgcaccattc ggagcgcctg cccgaaggcg ctgttggaca ccgcagtgac
    16741 cagcactgca tgcgcaccac caccggtcgc ctcgatcacg cgctgcgcgg catcgtccag
    16801 ccgcgcatcc accacgacct cggcaccgtc cgttcgcgca agggccagct tgtcctcagc
    16861 cacatcgatc gccaccaccc gcatgcccat tgcagtcgcg tactgcaccg cgaggtgccc
    16921 gaggccgccg atgccggaaa tcgcgatcca ttggcccgga cgcgctccgg tctccttgat
    16981 gcccttgtac gtggtcacac ccgcacacag gatcggcgcc atggccatcg gatcggaatg
    17041 ccccggcagc cgcgccacgt aggccgcgtt acccagcgcg tactccgcga agcatccgtt
    17101 cacgctgtat ccgctgttgt gctgcgtttc gcagagggtt tcccacccgg caacgcagtg
    17161 ttcacagtga ccacaggcat cgtgcagcca ggcgatgccg acgacatcat tttccttcag
    17221 atgatgaacg cccgggccgg ccgccaccac ccgaccgatc ccttcgtggc cggggatgaa
    17281 tggcaacgag ggtttgaccg gccaatcgcc gtccgcggca tgcaggtccg tatggcacac
    17341 gcctgatgcc gtgatgcgca ccagcacttc accgtaacca ggccttggga ccggccattc
    17401 ctcgatcgac agcggctgcc cgaagccgcg gacgaccgcg gcacgcatgg tctgcggaat
    17461 ggtcatgacg aatgtcctgt tcacttgacg ggtacgaccc atgtcagcac gcatccgcct
    17521 gttccttgtg atcccgatca agatcgccgc acctggagca cgcacaatgg gcatatccgg
    17581 atgaggaccg ctgccttgcc agccgacgcg agcgttgaga cccgccattc gtctttcgac
    17641 gtcctgctga aagccgcggc ggcactcggg cccattccca cggcggtggt ccatccctgc
    17701 gatccgctgt cgctgcaggc tgcgctggaa gcggcacgct ccggatcgat ccagccgatc
    17761 ctggtgggac cgcggtcgcg catcctgcgc gcggcggaat cggcgagcct ggacgtgggt
    17821 ggttgtcaca tcgaggatac gccgcacagc catgcggcgg cggcgcgcgc tgtcgggctg
    17881 gcacgcgccg gcgacgtcgc cgcccttgtg aagggtgcgc tgcataccga cgagctgatg
    17941 gccgaggtgg tggatcgcga acgcggcttg cgcaccgagc gccgcgtcag tcatgtcttc
    18001 gcgctggacg tgccacgcta cccgaagctg cttttgctga ccgatggcgc gctcaacatc
    18061 gcgcccgacc tgctggacaa gcgcgacatc atccaaaacg cgattgacct cgcccacgcg
    18121 atcggcatcg cgcttccgca agtcgcgatc ctttcggcgg ttgaaacgat caatccacgc
    18181 atcgcttcga ccatcgacgc cgcggccttg tgcaagatgc gcgaccgcgg ccagatcacc
    18241 ggcgggacac ttgaaggtcc cttggccttc gataacgcgg tatcggtcgc cgctgccgcg
    18301 atcaagggca tcgaatcccc ggtcgcgggc cacgccgaca tcctggtggc gcccgacctg
    18361 atgaccggca acatgctggc gaagcagatg gtgtatttgg ccggcgcttc atcggcaggc
    18421 gtcgtgctgg gcgcgcgcgt tccgatcgca ttgaccagca gggcggatgc ggtgcgtgcc
    18481 cgggtgatgt cctgcgcgct ggcgcggctg gtcgcccacc acgcctcgac cgttctgaca
    18541 tgagccgcgg catcctcgcg ctcaacatcg gctcgtccag catcaagctg gcgttgttcg
    18601 atgcggccac gctcgaaccg ctcctgcgcg gcaaggtcac ggatgtcgga cacgacccgc
    18661 atgccacctt gacgttgccc gggcattcca ccgtttcgga acgacctgcg gccgcggaca
    18721 tcggggaggc ggtccgctgg ttgctggccg agctgcgcga acggcattcg gaaatccgcc
    18781 tggccgcggt cggccatcgc gtggtccatg gcggcacccg tttcgacaag ccggtcagca
    18841 tcgacgaaaa cgtcctggcc gaactcgagg cgctggcggt gctggcgccg gcgcaccaac
    18901 cggccgagat cgcgctgatc aggaacgtgc aggaactgtt gccggacgtg ccgcaaatcg
    18961 cctgcttcga tacgtcattc caccgcaccc aaccgaagct gtcgcaatgg ttcgcgttgc
    19021 cgcgcgcatt gagtgaatcg ggcatcgtgc gcgtaggctt ccacggcctc tcgtacgaat
    19081 acatcgccag cgtgttgccg caaaccgccg gtacacgccc gtatgggaaa acggtggttg
    19141 cgcaccttgg ccacggcgcc agcgtgtgcg cgctgcgcga cctgcggagc gtttcgacct
    19201 cgatggggtt caccccgctg gatggcctga tgatggccac ccgcagcggc gcgatcgatc
    19261 ccggcgtgct gttgcatttg caacaacaac gcggaatgag cccgcaggag gtggcggacc
    19321 tcctgggcaa tcgttccgga ttgctgggcg tgtccgggat cagcgacgac gtgcgcgtgc
    19381 tggaagccag cgacgacccc tgcgcacgcg aagcgctgga aatgttcgcc gaacgcacgg
    19441 ccgctgcaat cgcggcgcag tgcgtggccc tggagggaat cgatgcgctg gtattcaccg
    19501 gcggcatcgg cgaacacgcc gaccgcacac gcaagcgcat cggcgaacgc ctggcatggc
    19561 tgggcgtcgc cctggacgat gcgcggaatg cgcggcatgg cgcacgcatc agtcctgacg
    19621 atgcgcggat ccaggtgctc gtgatcccga ccgacgagga aaggatggtc gcgcgtgcca
    19681 ccgcggccct gctggaatcg ccatcgcatc attgattgcg caatgcctgt ccgtgctgga
    19741 tcacgacgtt cgcgtttcag cgaatcagcg gccaagttga tcctggtcac aacgatcccc
    19801 atcgcgcgga ccatgctgct tccacaaaag ccaagcgagg tgctcgtcat gcgcacatca
    19861 ggaagcccgg ctcgggatca aacaaccacc gccaccgcgg tcagtgccgg caccagggcc
    19921 gccaagctgc ctgccccggc ggatgtcccg gaatcgcagg taaccttgct ggacgggagt
    19981 gaagtgacga ttcgggcgct gcggaaggag gacgcagagc tcgagcgcga attcatccgc
    20041 aacctgagcc cggaatcgcg ctggatgcgt ttcctcggcc aaatcagcga gcccagcgac
    20101 tcgctgatcc gcaagctgac caacctcgat taccagcgcg acatggcatt cattgcgctg
    20161 tcacaccaag gcgggggcac ccgcgaggtc ggcgtcagcc gttacagcct tgcgccggac
    20221 ggccaatcgt gcgagtgtgc ggtcactgtt gccgacgcgt ggcaaggcaa ggggctcggg
    20281 accgtcctga tgcgcgatct catcgatatc gcgcgccggc gcggcattcg ttccatgttt
    20341 tcggtggatg caagcgcgaa cgagcggatg cgggaattgg cgcgagacct cgggttcgaa
    20401 cgcaatcgag atccaggcga tcctacgcgg gtgatctacc ggcttgtgcc ctgaggacaa
    20461 gttcggccaa atgaggccaa gctgacctgg ccccaatccc cgtaccacgg ctaagcagga
    20521 gttgccacgc agcccttggt tcccgcgctc gtcgggttcc agcacgatcg cttcttcaac
    20581 gcatgccagc gcctcgtcgt tgcaggacca aaccgcacga ggttaggctg tgtcggcttg
    20641 ccaaactggc gacctcggat gaatcgagcg cgcgatcaaa cagcttcaga cgtgcgatct
    20701 tcccgacaaa gccgtctccg atactcaatg gaaagggatt cgctgccggc ttcttgtcgt
    20761 agcgggtttc gcgcgccaac gtgccatcaa cgtacaatcg caagagatcg ccatcgtacg
    20821 taacggccac atgatgccaa ccaggagcga gcttctgctc taccatcgct ccggccgtgt
    20881 cctccggggc ttcgccgtga taaaggtagt cttcgccggc attcacgaac cccgacacga
    20941 ttcccgacgc cgacatcccg atgccgtagg agtctccctt gctcaagacg ttggagcccg
    21001 agtaagccag aacgccattc tcctgcctca ccataccgcc ttcgtttttc acgtaatgaa
    21061 gtcggttcac gtcgtaatag gccttgcctc cgacgacgct caacatcttg tatcccgtca
    21121 ggtggtcgtc gccgtccccg ccaatcaggc gctgttgcac ccaatggtcg ccattcaggt
    21181 ccgacttggc tgtccagaaa gcgaagccca tcaacccggt ttttctcatc accgcaatgg
    21241 gctgctgcat gaccgaaaat gcgtattgaa tcttgtcgcc tacaacttgc acccccgcag
    21301 gtatggagtt gcccacgccg aacgtcctct ggctctcctt gaacccggaa ccgtccgtcg
    21361 ccacgttgcc ggtccacaag tggacgcttt tgtccgggtc accttttgca tatgaaaaat
    21421 agatgacgcc cttgtcgacc gtgagctggg cgatgatcca ccatgcgcca ccatgaatcc
    21481 ggcgaagttc atgccatccc gacccgtcga ggtttgcgga cgcaatgacg atgtcgtaga
    21541 ttttcttttt tgccgtaggc gcaggaaaga tgtaatagat cttgttgcct gacaccttga
    21601 agctcggaat gatccctccg cttgtcgtct gttgccgtgc ctgccaggac gatccatcca
    21661 cgttggatgt cgccgtccac aattgccact ttccagcctt gtctttcatc ggaaaggcgt
    21721 ggtagacctt gtttccaacg acctgaatat ttcgggtctg ctcggtgtcg tagcgtccct
    21781 tcgtgaaggt cctctgggtc gctcgccaac ccctgcc
//
LOCUS       DASSNH010000227         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_393962, whole genome shotgun sequence.
ACCESSION   DASSNH010000227 DASSNH010000000
VERSION     DASSNH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393962"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFK00_08250"
     CDS             <1..885
                     /locus_tag="VFK00_08250"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET7592977.1"
                     /translation="HVVLQRDIRIPVKVQCRVDPATHQGCVLVARAAASAKGVTADRT
                     FRTWGRRLLTGRPGACHHPPGARRRGGVVEDPREPRILNIAEIGALAHLYRGEVYRST
                     VWRTRLDTTTNWAVVTLGVALSITFASPDASPLPLLLVGILILFFLVLEARRYRYFNV
                     WRARCRWLEVHFFAPLLDGGEPRMDGWEKRLADDYRRPDYHVSTLVATGRRVRHNYLW
                     ILAIQAAAYAGKIIVHPARVQTMGQFIERAAIGPVPGLVVLSVGIAYILSWLGLAVWS
                     WADDARRARDRQGRGGMG"
     gene            complement(1002..1991)
                     /locus_tag="VFK00_08255"
     CDS             complement(1002..1991)
                     /locus_tag="VFK00_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7592978.1"
                     /translation="MHVPLWIWLATIVGVIALLALSFFAHIRDAHEPSMKEAGAWTAF
                     FVSLSVVFGVGLTLVWGHDHGVEFFAGYITEYSLSIDNLFVFVIIMSSFAVPRAFQQK
                     VLLVGIIVALILRTIFILIGAAAIAHFSWVFYIFGLFLILTALKLAFEKHDKDETYEP
                     NILVRAVQKILPATDEYDGANVTTVIDGKRYMTPMMMVMLAIGMTDILFALDSIPAIY
                     GLTSEPYIVFTTNAFALLGLIELYFLIGGLLKKLVYLSLGLAVVLGFIGIKLIIEAMH
                     GNSLPFLNGGEPIHVVPAIPTWLSLVIILSILAVTTIASLIKSRREAAATGRP"
     gene            complement(2042..3349)
                     /locus_tag="VFK00_08260"
     CDS             complement(2042..3349)
                     /locus_tag="VFK00_08260"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7592979.1"
                     /translation="MTKHLRILACLFTALPTIAVAAAPPAKDAELAARVERVLTATPI
                     IDGHNDLPWGIRSEFGNIGNIDLAADTSQLRSKTGDDDVHLMTDIPRLRAGHVGAQFW
                     SVWIPATTTGPDAVKTTLEQIDIVRTMCRKYPESFEMAYTADDIERIEKAGRIACLIG
                     VEGGHQIDNSLAVLRDYYALGARYMTLTHGKTTDWADSATDEPTHQGLTAFGRAVVRE
                     MNRLGMLVDLSHVSPDTMKDALETTRAPVIFSHSSARALVDHPRDVPDEVLRLVRQNH
                     GIVMVNFYPTFVSEAVARWHADRAGVAARARALYVGQPQRAKAAIDQWEQEHPQPKAT
                     IAQVADHIDHVRKVAGIESVGIGSDFDGIETTPDGLDGVDKYPALFEELARRGWTDDE
                     LADLAGRNLLRVMQEAEAVAGRMQADEVPSHATIEMDAKDDRA"
BASE COUNT          567 a         1064 c         1190 g          633 t
ORIGIN      
        1 catgtggtgc tgcaacgcga tattcgaatc ccggtgaagg tgcaatgcag ggtcgatcct
       61 gcaacgcatc agggatgtgt actcgttgcg cgggccgccg cgtcagcaaa aggagtaact
      121 gccgatcgaa ccttccgaac atgggggcgc cgtcttttga ccgggcggcc gggtgcgtgc
      181 catcatccgc ccggggcacg gcgtcgcgga ggcgttgtgg aagacccgcg cgaaccaagg
      241 atcctgaaca tcgccgagat cggtgcgctg gcccaccttt atcggggcga ggtctaccgt
      301 tccacggtct ggcgcacccg gctcgacacg accaccaatt gggctgtcgt cacgctaggc
      361 gttgcattgt cgatcacctt cgcgtccccg gacgcatctc cgcttccact cttgctggtg
      421 ggcatcctca tcctgttctt tctcgtgctc gaagcgcggc gttaccgcta cttcaacgtc
      481 tggcgggcgc gctgccggtg gctggaggtg cacttcttcg cgccgctcct cgacggcggc
      541 gagccccgga tggacggctg ggaaaaacgc ctggccgacg attatcgccg gccggattac
      601 catgtcagca cgctggttgc gaccggccgc cgcgtgcgtc acaactatct ctggatcctt
      661 gcgatccagg cggcagccta cgccggaaag atcatcgtgc acccggcgcg cgtgcagacg
      721 atgggacagt tcatcgagcg ggccgcgatc ggcccggttc cgggcctcgt cgttctttcc
      781 gtcgggatcg cctacatcct gagctggctg ggcctggcgg tctggagctg ggccgacgac
      841 gcacggcgcg ccagggaccg gcaaggccgc ggcggcatgg gttagcggcg cggtccaccc
      901 ggcggcccat gcgcccatcg catgtgcaag agggcgcgac gatccgccac gacggcgccg
      961 gcgcgcgaag cctcgattcg ccgcacgtcg tggacatcgg ctcagggacg gccggtggcc
     1021 gcggcttcgc gccggctctt gatcaggctg gcgatggtgg tgacggcaag aatggagagg
     1081 atgatcacca gcgacagcca ggtcgggatg gccggcacca cgtggatcgg ctcgccgccg
     1141 ttgaggaacg gcaggctgtt gccgtgcatg gcctcgatga tgagcttgat gccgatgaag
     1201 ccgagcacga cggccagccc gaggctgagg tacaccagct tcttgagcag gccgccgatc
     1261 aggaagtaca gttcgatcaa gccgagcagg gcgaaggcat tggtggtgaa cacgatgtac
     1321 ggttcgctgg tgaggccata gatcgcgggg atcgaatcca gggcgaacag gatgtcggtc
     1381 atgccgatcg ccagcatcac catcatcatc ggggtcatgt agcgcttgcc gtcgatgacc
     1441 gtggtcacgt tcgcgccatc gtattcgtcg gtcgccggca ggatcttctg gacggcgcgc
     1501 accaggatgt tgggttcgta ggtctcgtcc ttgtcgtgct tttcgaatgc cagcttgagc
     1561 gccgtcagga tcaggaacaa cccgaagatg tagaacaccc aactgaagtg cgcgatggcc
     1621 gccgcgccga tcaggatgaa gatcgtgcgc aggatcagcg ccacgatgat gccgaccagc
     1681 aggaccttct gctggaacgc ccgcggcacc gcgaagctcg acatgatgat cacgaacacg
     1741 aacaggttgt cgatcgacag gctgtattcg gtgatgtagc cggcgaagaa ctcgacgccg
     1801 tgatcatggc cccatacgag cgtcagcccg acgccgaata ccaccgacag cgaaacaaag
     1861 aaggcggtcc atgccccggc ttccttcatg gaaggttcgt gggcgtcgcg gatgtgcgcg
     1921 aagaagctga gcgccagcag cgcgatcacg ccgacgatcg tggccaacca tatccagagg
     1981 ggtacgtgca tgggcagggg cggaagcagt cacgcggaca ggtgttgcct gtgtccgctt
     2041 ctcaggctcg gtcgtctttg gcgtccatct cgatcgtggc atgcgacggc acttcatcgg
     2101 cctgcatccg cccggcgacg gcttcggcct cttgcatcac gcgcagcagg ttgcgcccgg
     2161 cgaggtcggc cagttcgtcg tcggtccagc cgcggcgcgc cagttcctcg aacagggcgg
     2221 gatacttgtc gacgccatcc aggccatcgg gtgtggtctc gatgccgtcg aagtcgctgc
     2281 cgatgccgac actttcgatg cccgcgacct tgcggacgtg gtcgatgtgg tcggcgacct
     2341 gcgcgatcgt ggccttcggc tgcggatgtt cctgctccca ttggtcgatg gcggccttgg
     2401 cgcgctgggg ttggccgacg tacagcgccc tggcgcgcgc tgcgactccc gcgcggtcgg
     2461 cgtgccagcg cgcgaccgct tccgaaacga aggtgggata gaagttcacc atgacgatgc
     2521 cgtggttctg cctgaccagg cgcaagacct cgtcggggac gtcgcgcggg tggtcgacca
     2581 gcgcgcgtgc gctggagtgc gagaagatca ccggcgcccg ggtcgtttcg agtgcatcct
     2641 tcatggtgtc gggcgacacg tgggagaggt ccaccagcat ccccagccgg ttcatctcgc
     2701 gcaccaccgc gcggccgaag gcggtgagtc cctggtgcgt gggctcatcg gtggccgaat
     2761 ccgcccagtc cgtggtcttg ccgtgggtca gcgtcatgta gcgcgcgccc agcgcgtagt
     2821 agtcgcgcag caccgccagc gagttgtcga tctggtggcc accctcgacg ccgatcaggc
     2881 aggcaatgcg gccggccttt tcgatgcgct cgatgtcgtc ggcggtgtag gccatctcga
     2941 acgattccgg gtacttgcgg cacatcgtgc ggacgatgtc gatttgctcc agcgtcgtct
     3001 tcaccgcgtc gggtccggtg gtcgttgcgg gaatccagac cgaccagaat tgtgcgccga
     3061 cgtggcccgc gcgcaggcgc ggaatgtcgg tcatcaggtg cacgtcgtcg tcgccggtct
     3121 tgctgcgcag ttgggaggta tcggcggcga ggtcgatgtt gccgatgttg ccgaattcgc
     3181 tgcggattcc ccacggcagg tcgttgtggc cgtcgatgat gggcgttgcc gtgagcaccc
     3241 gctcgacccg ggcggccagc tccgcgtcct tggcgggcgg cgcggcggcg acggcgatgg
     3301 tgggcagggc ggtgaacagg caggcaagga ttcgaagatg cttggtcatg gcgtcccgat
     3361 aatgtcacga ggagaggtgc acgttgcatt gcacgccgca tggttcgaag gtcgagccta
     3421 ccatcgtttt cggggcattc agaaaaacgt cggc
//
LOCUS       DASSNH010000228        14479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1518469, whole genome shotgun sequence.
ACCESSION   DASSNH010000228 DASSNH010000000
VERSION     DASSNH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518469"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFK00_08265"
     CDS             complement(<1..878)
                     /locus_tag="VFK00_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592980.1"
                     /translation="MCESQTYSEAPGSELVVERSASAEARIASRPGLRLTAADRPGQA
                     QPVPQRDVPDRRWGAIMLGALAIFLLLMGAWEWHWRGYGVEPSIANTDALWATQRRRI
                     DNGEGDGATVLIGSSRMLFDLQLPVWQKLSGTRPIQLAMEGTSPMFALEDLASDPDFR
                     NGRLLVGIAPDLFFSGFMVRDNLLAYTRKQSPSQRVGQWLSMKFVEPHFAFDDPDYAL
                     PTVLARQAWWPARPGMHAAKPVRKLMVVVDEDRASHIWDKLEKDPAYATLAQSIWAQH
                     FTLLRGMPAPVLQKSI"
     gene            complement(994..2409)
                     /locus_tag="VFK00_08270"
     CDS             complement(994..2409)
                     /locus_tag="VFK00_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET7592981.1"
                     /translation="MVFNSLTFIAFFAIVLAIHSLPLAWKIRKVNLLVASYLFYSAWN
                     PPFVILLWISTVVDWYAAQGLVRAQRPVARRAWMLLSVVANLGMLGYFKYGGFLLANF
                     TALLASIGMTYHAPAPDIVLPVGISFYTFATMSYTLDVYLRRALPARNFLDYALFVTF
                     FPHLVAGPIMRPTELVPQFEQPRRANRQQLFFGLALMTIGMFNKVVLADGFLAHAAEA
                     VYDTDKIPGALDAWMGTLAFSGQIFCDFAGYSTIAIGVAMCLGFAMPDNFRFPYAAIG
                     FSDFWRRWHITLSSWLRDYLYIPLGGNRHGPRRTYAALMGTMLLGGLWHGASWTFVAW
                     GGLHGIYLSVERRLRQRFRGFRPNLWTLLAFALVTYALVNVTWVFFRAKTFERAWTVL
                     RGMAGTNDKPVPLLGTESLIVVAAIIAGLVVVHWLMRNRTLESVLARAPAAVLSCTWA
                     VMAFAIIATQGNGNAFIYFQF"
     gene            2546..4051
                     /locus_tag="VFK00_08275"
     CDS             2546..4051
                     /locus_tag="VFK00_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7592982.1"
                     /translation="MRTRYRGPMLGVQRPPCEEGVIRSRPRIGTARRAHAGLALAASV
                     LGSSIAFIDGSVVNVALPTLQREMAAGLGAMQWVVNAYLLMLGALVLVGGVLGDRLGQ
                     RTVFTWGIVVFTLASVACGLAPDAGVLIAARALQGIGAALLVPSSLAIIAAVYPEAER
                     GKAIGTWAAAGAITTAIGPVLGGWLVDAVSWRAIFFINVPLAIAAVVLAHAAVPNQRS
                     GESERLDWPGAILAIAGLALLTWALIAWPDRTSNALMIDAALVLGVAALALFVVIEAR
                     SRAPMVPLGLFRSHDFSGANLVTLLLYFALGGALFFLPFNLIRAHGYSATAAGASLLP
                     LSILMGALSPTAGALATRFGARMVLTVGPALAALGLALLAVGATTENYWRGTFPGITV
                     LGLGMTIAVAPLTATVMGAVPAGQAGVASGINNAVARMASLLAVAVLGLLFRAPADAA
                     APAGTTAGFRAVAAGAALCAIAAAVCALAILRPTPTQKSRQTRAGRSPHES"
     gene            complement(4080..4868)
                     /locus_tag="VFK00_08280"
     CDS             complement(4080..4868)
                     /locus_tag="VFK00_08280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7592983.1"
                     /translation="MHKPGFSLIAALLLPLAATAQSTAPAPEPAMTASNVPAYITRAI
                     NDPARAADRKDDARRKIAEVMAFTGVKPGDKVLELVPGSGYWTRVFSKIVGPDGHVYT
                     VWPDEMAKYAEKSYAEWQGLVKDTYTNVSLLKQPGATLAVPQKVDVVFTSQNYHDYHD
                     PFMGPVDMKKFDKEVYDALKPGGVFVVIDHAAPAGSGLNDTNTLHRIDPAVVKQEVEA
                     VGFVFDGSSDALVNPKDPLDIKVFDPSIRGHTSQFMYRFRKPAQ"
     gene            complement(4973..5383)
                     /locus_tag="VFK00_08285"
     CDS             complement(4973..5383)
                     /locus_tag="VFK00_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592984.1"
                     /translation="MGSASYEVVGRVACFRLAGEQELEDGVHRITDAILRTKQLGLEK
                     LLVDITAITGVEPPSVDTRYWLMGEWADAGRGSVRVAIVTRPEFIAPDRFAVIFGMNR
                     GFINNVFETEAQALEWLHGHGGRADPGKGGAGSG"
     gene            complement(5447..6505)
                     /locus_tag="VFK00_08290"
     CDS             complement(5447..6505)
                     /locus_tag="VFK00_08290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7592985.1"
                     /translation="MSSGALPVPTRVIADELRRAMRRSHVLRALGLGLGAVMVGTVLY
                     RHHAPAPVWGLLALYVLVWPHAAWLLARRSERPVAADRRWMAVDAAMMGVWIALMQFN
                     LLPSVLLAVMFAMTLVAIGGGALLLRGTLVQVVACGITAALNGLAAEPVTGIPELLAA
                     IPLLVVFPMALSVIMYGLSQRVRQQNRVLREMSSIDGLSGLFNRRHWEGVVDALLAAR
                     HRPAAVMLLIDIDHFKRVNDQHGHTAGDDVIRMVGATIRTSLREGDLAGRYGGDEFCV
                     VLRDIDAIAAERVAERIRSRIACSLLENVPGLHCTVSIGVAQDDPEFRSVSDWVKEAD
                     GALYRAKLAGRNGFAVAS"
     gene            complement(6744..8984)
                     /gene="bglX"
                     /locus_tag="VFK00_08295"
     CDS             complement(6744..8984)
                     /gene="bglX"
                     /locus_tag="VFK00_08295"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HET7592986.1"
                     /translation="MIRIAFAVLAILWLAPSASALAADAQPLRHDDKAMQSFVDGLLQ
                     RMTIEEKVGQLTILGADDKDLEQLVPEGKVGGTNGVLQDTDVVAYTHRMQQLAMQSRL
                     KIPLWFMGDVCHGFATLFPVPLAMAASWDPALVQQVDRAAATEATAAGVDWTFCPMVD
                     ISRDPRWGRVVEGAGEDPFLGSAMAAAQVRGFQGEDLAAPDTMMATVKHFAGYGAVQA
                     GRDYNSAWIPPREFRDVYLPPYRAAVDAGARSVMAAFVALDYVPATADRELLTGILRD
                     HWGFKGLVVSDYDAVPELQQHGIARDPAEAARLALHAGVDIDLHSGTYLKELPALVRS
                     GRVPEAELDAAVRRVLVAKYKLGLFDDPFRYGNRATAERVTLSPRHRELARRIARESM
                     VLLENRDGTLPLQRRGRIAVIGPLARARETLQGPMPAVAKPRDVVTILDGIREVAGND
                     VDVAFAKGVDVHGDDMSGIEGAVKLARSAGIAVLVLGEDRPMIGEGNSRAHIDLPGRQ
                     LELARAVKAAGVPIVVVLVNGRPLTIPWLHEHADAVLEAWLPGDEGGHAVADLLFGEE
                     NPSGKLPITFPRRLGQVPIHYAHLETGRPFDPAQPAYTSRYVDVPNTPLYPFGYGLSY
                     TTFAFGPVLLDSHALAPGDTLHATVRVTNTGKRAGTEVAQLYIRDEVADVSRPLRQLR
                     GFRRVTLAPGESRDVTFTITPSMLAFHRLDMSFGTEPGRFDVYVGADSGASDRASFTL
                     EAAGQP"
     gene            9167..10036
                     /locus_tag="VFK00_08300"
     CDS             9167..10036
                     /locus_tag="VFK00_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7592987.1"
                     /translation="MSHPAASSKAVLITGCSTGIGKATAERLARGGWTVYATARKPET
                     LEDLKATGCRTLALDVRDEGSMQAAVHAVEEAHGAVGVLINNAGYSQSGAVESVPLSK
                     VRAQFETNVFGLVRMCQLVLPGMRRRGLGRIVNMGSMGGKLTFPGGGIYHATKYAVEA
                     LSDALRFEVRGFGVDVVLIQPGLIRSRFSATVGEAIDAVQADAGPYEAFNAAVAKSTR
                     DAYENGLLARLGGDPEAVAAVIERVLRSARPRARYTVTPSARLMMTQRALMSDRLWDR
                     FLRGNFPTPGSPG"
     gene            10045..10737
                     /locus_tag="VFK00_08305"
     CDS             10045..10737
                     /locus_tag="VFK00_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7592988.1"
                     /translation="MHGARARKHPPAIRRRAVSDDQKARRRRAILEAALELFDAVGFE
                     RVSMAAVAARIRLAKGTLYLYFPTREALFLALLTEQFEAFFDALDAALERPLSRSAFV
                     DHLVRDLRDRPRLLRLIAVMHTVLERNVDLDTAIGFKRFLATHAQHSGARIDARLRGV
                     PAGFGLRLLLWLHALAIGLHHMATPAPVVRQAIQADPRLAVFDIDFARELRAMLIRLL
                     QAPAGSTLGLDG"
     gene            complement(10741..11502)
                     /locus_tag="VFK00_08310"
     CDS             complement(10741..11502)
                     /locus_tag="VFK00_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7592989.1"
                     /translation="MNLHGVRAIYRFEMARTGRTLMQSVLSPVVSTSLYFVVFGSAIG
                     SRMSSIDGVPYAAFLVPGLVMLSLLMQSVSNASFGIYFPKFVGTIYEVHSAPLSYLEV
                     VMGYVGAAATKAILVGLIILGTARLFVDYSIAHPLVMVAFLVMTTVTFSLFGFIIGIW
                     ADNFEKLQVVPLMVITPLTFLGGSFYPIHVLPPLWQKVTLLNPVVYLVSGFRWSFYGL
                     ADVNVGISVAATLGFLLLCLAGVWWIFRTGWRLKA"
     gene            complement(11597..12556)
                     /locus_tag="VFK00_08315"
     CDS             complement(11597..12556)
                     /locus_tag="VFK00_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7592990.1"
                     /translation="MNETLSPPRAATDPVIEVRGLDKTYKGGFQALKGIDLTVHRREI
                     FALLGPNGAGKTTLISIICGIVNPGHGSITVDGHDIIREWRKTRSMIGLVPQELTTDA
                     FESVWDTVSFSRGLFGKAPDDAYIEQLLKDLSLWEKRDNKILMLSGGMKRRVMIAKAL
                     SHQPTVLFLDEPSAGVDVELRRDMWAMVRRLRESGVTIILTTHYIEEAEDMADRVGVI
                     NKGELIVVEEKTTLMQKLGKKQLRLQLNEPIDALPESLSAYALDLNGGRDELVYTYDT
                     RRERTGITSLLKDLSAAGIGFKDLSTRQDSLEQIFVDLVREGP"
     gene            complement(12720..14036)
                     /locus_tag="VFK00_08320"
     CDS             complement(12720..14036)
                     /locus_tag="VFK00_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7592991.1"
                     /translation="MKRILPLLLCLFAWPALAVAPKPLLLTHVGLIDGSGSPVQRDMT
                     IAIEGQQITAVYPGDSLPAPKDAEVRDLSGKYVIPGLIDAHVHITDAEPDIAHYQTFL
                     HALLLGGVTGVRDMAGDDRLLQFLADQANSDTFASPDIFYVALMAGPTFFKEDFRAQD
                     ASKGEPLGFAPWMQAITAKTDVRLAVAEAKGTGATGIKLYANLPAALVEAITAEARRQ
                     GLRVWTHATIFPAKPSDAVDAGVDTISHSAYLVWEAAPSVPDDYRMRAMGDFEHIKPA
                     APRILALFDAMKKRGTILDATLLVFKNEAEHHPQRVGGGIMPWSYAVTKLAHQRGVLV
                     DAGTDDAGFAYGKDGPELDQPPSVHEEMALLVEHAGFTPLQAIEAATEVGAGALGQSA
                     KRGTVTPGKLADLVVLDADPSQDIHNTTKIDFVVKHGRIYTRQAEH"
BASE COUNT         2449 a         4905 c         4726 g         2399 t
ORIGIN      
        1 tcgatgctct tctgcagcac cggcgccggc atgccgcgca gcagggtgaa gtgctgcgcc
       61 cagatgctct gcgccagcgt ggcataggcc ggatcctttt ccagcttgtc ccagatgtgg
      121 ctggcacggt cctcgtccac caccaccatc agcttgcgga ccggtttggc ggcgtgcatg
      181 ccgggacgtg ccggccacca cgcctgccgc gcgagcaccg tggggagcgc gtagtcgggg
      241 tcgtcgaacg cgaaatgcgg ttcgacgaac ttcatcgaaa gccattgccc gacacgctgc
      301 gagggtgatt gcttgcgcgt gtacgccagc aggttgtcgc gcaccatgaa gccgctgaag
      361 aacaggtcgg gtgcaattcc gaccagcagc cggccattcc tgaaatccgg atcgctggcc
      421 aggtcctcga gcgcgaacat cggcgaggtg ccttccatcg cgagctggat cggtcgagta
      481 cccgagagct tctgccacac cggcagttgc aggtcgaaca gcatgcgcga ggaaccgatc
      541 agcacggtcg cgccgtcacc ttcgccgttg tcgatccgtc ggcgctgcgt cgcccacagc
      601 gcgtcggtat tggcaatgct cggttcgacg ccgtagccgc gccagtgcca ttcccaagcg
      661 cccatcagca gcaggaagat cgccagtgca cccaacatga tcgcgcccca gcggcggtcg
      721 ggaacgtcgc gctggggtac cggctgggcc tggcccgggc ggtccgccgc ggtcaaccgc
      781 agcccgggac gcgaggcaat gcgtgcctcg gcgctcgccg accgctcgac gaccaattcg
      841 gaaccgggcg cttcagaata cgtctgactc tcgcacatcg cagtgggttc cgcttcagtc
      901 cccttgagtc aaggggacgg aatcgtcgcg aagcgacggt ttcggggtta tggaggtatg
      961 acgcccttgg ataggcggca ctcgtcgatt gctttagaac tgaaaataaa taaatgcatt
     1021 gccattgccc tgcgtcgcga tgatcgcgaa cgccatgact gcccaggtgc acgacagcac
     1081 cgccgccggc gcgcgcgcca gcaccgattc cagcgtgcgg ttgcgcatca gccagtgcac
     1141 gacgaccagt cccgcgatga tcgcggccac cacgatcagc gattcggtgc cgagcagtgg
     1201 caccggcttg tcgttggtgc cggccatgcc gcgcagcacc gtccatgcgc gctcgaacgt
     1261 tttcgcacgg aagaacaccc aggtcacgtt gaccagcgcg taggtcacca gtgcgaatgc
     1321 cagcagcgtc cacaggttcg gccggaatcc ccggaagcgc tggcgcaagc gacgttcgac
     1381 ggaaaggtag atgccgtgca agccgcccca ggcgacgaag gtccagctcg cgccatgcca
     1441 aaggccgccg agcagcatcg tccccatcaa tgctgcgtag gtgcggcgcg ggccgtggcg
     1501 attgccgccg agcgggatgt acaggtaatc gcgcagccac gacgacagcg tgatgtgcca
     1561 gcgccgccag aagtcggaga acccgatcgc ggcatacggg aaacggaagt tgtccggcat
     1621 tgcgaacccg aggcacatcg ccacgccgat cgcgatcgtg gaatagcccg cgaagtcgca
     1681 gaagatctgg ccgctgaagg cgagcgtccc catccacgca tccagggcgc cgggaatctt
     1741 gtccgtgtcg tacaccgcct ccgccgcatg cgcgaggaag ccgtcggcca gcaccacctt
     1801 gttgaacatg ccgatggtca tcagcgccag cccgaagaac agttgctggc gattcgcgcg
     1861 gcgcggctgc tcgaactgcg gcaccagttc ggtcggccgc atgatgggac cagcgacgag
     1921 gtgcggaaag aacgtcacga acaacgcgta gtcgaggaaa ttgcgcgccg gcaatgcgcg
     1981 gcggaggtat acgtcgagcg tgtaggacat cgtcgcgaag gtgtagaacg agatccccac
     2041 cggcagcacg atgtccggcg ccggcgcgtg gtaggtcatg ccgatcgatg ccagcagcgc
     2101 ggtgaaattc gccagcagga atccgccgta cttgaagtag ccgagcatcc cgaggttggc
     2161 gaccaccgac agcaacatcc aggcgcggcg tgccaccgga cgctgcgccc gaaccagccc
     2221 ctgcgcggcg taccagtcca ccacggtcga gatccacagc aggatcacga acggcggatt
     2281 ccacgcggaa tagaagaggt agctcgcgac cagcaggttg accttgcgga tcttccacgc
     2341 gagcggaagc gaatggatcg caagcacgat cgcgaagaac gcgatgaacg tcagcgaatt
     2401 gaagaccatg caggcctccc cgcccggggc cggaggcggt ccgcaggcgg cgggagtatg
     2461 tcacaaccgg tgggctgcgc cagtgcctgc cgagcgggcg ctccgtgtgc gtcgctggaa
     2521 ccgccacaag gcagatgttg ccgccatgcg aacccgctac cgtggcccga tgctcggtgt
     2581 ccagcgtccc ccgtgcgaag aaggcgtcat ccgcagccgg ccgcgcatcg gcacggcacg
     2641 tcgcgcgcat gcgggactcg cgctggcggc gagcgtcctc ggctcgagca tcgccttcat
     2701 cgacggctcg gtggtcaacg tcgcgctgcc cacgctgcag cgcgagatgg ctgccgggct
     2761 gggcgcgatg caatgggtgg tcaacgccta tttgttgatg ctcggcgcac ttgtgctggt
     2821 gggcggggtg ctcggcgacc gcctcggcca acgcacggtc ttcacctggg gcatcgtcgt
     2881 gttcacgctg gcctcggtgg cctgcggcct tgcgccggat gccggcgtac tgatcgcggc
     2941 acgtgcgctg caaggcatcg gtgccgcgct gctggtgccg agcagcctgg ccatcatcgc
     3001 cgccgtgtac ccggaagccg agcgcggcaa ggcgatcggc acctgggcag cggcgggtgc
     3061 gatcaccacc gcgatcggcc cggtgctggg cggctggctg gtcgatgcgg tgtcgtggcg
     3121 cgccatcttc ttcatcaacg tgccgctggc gatcgccgcc gtcgtgctgg cgcacgctgc
     3181 cgtgccgaac cagcgcagcg gcgaatccga acgtctcgat tggccgggtg cgatattggc
     3241 catcgccggc ctcgccctcc tgacgtgggc gctgatcgcg tggccggacc ggacttcgaa
     3301 cgccctcatg attgacgccg cgctggtact cggcgtcgcc gcgctggccc tgttcgtggt
     3361 gatcgaggcg cgcagccgcg cacccatggt gccgctgggc ctgttccgtt cgcacgactt
     3421 ttccggcgcc aacctggtca cgttgctgct gtacttcgct ctgggcggtg cgctgttctt
     3481 cctgccgttc aacctgatcc gcgcgcacgg ctattcggcg acggcggctg gcgcaagcct
     3541 gttgccgctt tcgatcctca tgggcgcgct gtctccgacc gccggcgcgc tggccacgcg
     3601 tttcggtgcg cgcatggtat tgaccgtggg tccggcgctg gcggcgctcg gcctggcatt
     3661 gctggccgtg ggcgccacga ccgaaaatta ctggcgcggt acttttcccg gcatcaccgt
     3721 gctcggcctg ggcatgacca tcgcggtcgc gccgctgacc gccaccgtga tgggcgccgt
     3781 gcccgccggg caggccggtg tcgcgtcggg aatcaacaac gccgttgcac gcatggccag
     3841 cctgctggcc gtggcagtgc tgggactgct gttccgcgca ccggccgatg cggcggcgcc
     3901 ggccggaacg accgccggct ttcgcgccgt cgcggccggc gccgcactct gcgcaatcgc
     3961 ggcggcggtg tgcgcgctcg caatcctccg cccgacgcca acgcaaaaaa gtcgacaaac
     4021 gcgggccggg cgatcaccac acgaatcgtg aggagcccgg cccgccgtgc attccggttt
     4081 tattgcgcgg gtttgcggaa ccggtacatg aactggctgg tgtggccacg gatcgacgga
     4141 tcgaacacct tgatgtcgag cgggtccttc gggttgacca gcgcatcgct ggaaccgtcg
     4201 aacacgaagc ccaccgcttc gacttcctgc ttgaccaccg cgggatcgat ccggtgcagc
     4261 gtattggtgt cgttcaaccc ggacccggca ggcgctgcat ggtcgatcac gacgaacacg
     4321 ccgcccggct tcagcgcgtc gtacacttcc ttgtcgaact tcttcatgtc caccggaccc
     4381 atgaacgggt cgtggtagtc atggtagttc tgcgaggtga acacgacatc gaccttctgc
     4441 ggcacggcca aggtcgcccc gggttgcttc aacaggctga cgttggtgta ggtgtccttt
     4501 accaggccct gccactccgc atagctcttt tccgcgtact tggccatttc gtccggccat
     4561 accgtgtaca cgtggccgtc gggtccgacg atcttgctga acacgcgcgt ccagtagccc
     4621 gatcccggaa ccagctcgag caccttgtcg cccggcttca cgccggtgaa cgccatcacc
     4681 tccgcgatct tgcgccgcgc gtcgtccttg cggtcggcgg cgcgtgcagg atcgttgatc
     4741 gcacgggtga tgtaggccgg cacgttgctg gccgtcattg cgggctcggg cgcgggagcc
     4801 gtcgactgcg cggtcgccgc gagcggcaac agcaaagcag cgatcaagga aaaaccgggc
     4861 ttgtgcatgg caatctcctt gcggtcaggg atggccattg tgctaccaaa ccggcgagcc
     4921 tgccgaagcc agcggatcgc ggcattggca gcgggccgaa cggtcgggct ggtcagcccg
     4981 acccggctcc gcctttcccc gggtcggccc tgccaccgtg gccatgcagc cactcgagcg
     5041 cctgcgcctc ggtctcgaag acgttattga tgaacccgcg gttcatgccg aagatcacgg
     5101 cgaagcggtc cggcgcgatg aactcgggcc gggtcacgat cgcaacccgc acgctgcccc
     5161 gaccggcgtc cgcccattcg cccatcagcc agtagcgggt atccaccgat ggcggctcga
     5221 cgccggtgat cgcggtgatg tcgacgagca gcttttccag cccgagttgt ttggtccgca
     5281 ggatggcatc ggtgatccgg tgcacgccat cctccagttc ctgctcaccc gccagacgga
     5341 agcaggcaac ccgtccaacc acttcgtacg acgcgctccc catggggcac cttgccggcg
     5401 aagccctgaa cctggctaga tggtagcatc cgccgggggc gccgcatcaa ctcgccacgg
     5461 caaacccgtt gcggccggca agcttggccc tgtacagcgc gccatcggct tccttcaccc
     5521 agtcgctgac gctgcggaac tccgggtcgt cctgcgccac cccgatgctc acggtgcaat
     5581 gcagcccagg cacgttctcg agcagcgagc aggcgatccg cgaccggatc cgctcggcca
     5641 cgcgctcggc ggcgatggca tcgatgtcgc gcagcaccac gcagaactcg tcgccaccgt
     5701 agcggcccgc gagatccccc tcgcgcaggc tggtgcggat agttgccccg accatgcgga
     5761 tgacatcgtc gccggcggtg tggccgtgct ggtcgttgac ccgcttgaag tggtcgatgt
     5821 cgatcagcag catcaccgcc gcgggccggt ggcgcgccgc gagcagcgcg tccaccacgc
     5881 cctcccaatg cctgcggttg aacaggccac tgagcccgtc gatgctgctc atctcgcgca
     5941 gcacgcggtt ctgttgccgg acccgttgtg acagcccgta catgatgacg ctcagtgcca
     6001 tcgggaagac gaccagcagg ggaatggcgg cgagcagctc cggaatgccg gtgaccggct
     6061 cggccgccag gccgttgagc gcggccgtga tgccgcaggc gaccacctgt accagcgtgc
     6121 ctcgcagcag caacgctcca ccacctatcg ctaccagcgt catggcgaac atcaccgcca
     6181 gcagcacgct cggcaacagg ttgaactgca tcagcgcgat ccatacaccc atcatcgcgg
     6241 cgtccaccgc catccagcgc cggtcggcgg ccaccggtcg ttcgctccgg cgcgccagca
     6301 gccatgccgc gtgcggccac accaagacgt acagtgcgag cagcccccac accggtgccg
     6361 gcgcgtggtg ccggtacagc accgtgccca ccatcacggc cccgagtccc aagcccagcg
     6421 cgcgcaacac gtgcgagcgc cgcatggcgc gccgcagctc gtcggcaatt acccgggtcg
     6481 ggactggcaa ggcaccgctg gacatcggaa tcgggcccct cgaaataaga cgctcgatta
     6541 cgggtgagca aaatgggtgc cacgcggcga ctacctgcgg gttcgacccg cggttcgcat
     6601 gcggaaatgt gatcggcgca ctcccccgaa acccttgttg accgatcgac ccgtggcgtg
     6661 cgtcccgttc gcatgcgtcc agccaatgtg ccgaactgtg aacattccgg cggctgcgcc
     6721 agagctcgtg cgatggatat gcgctacggc tgccctgcgg cttcgagcgt gaatgatgcg
     6781 cggtcgctcg cgcccgaatc ggcgcccacg tacacgtcga aacgacccgg ttcggtgccg
     6841 aagctcatgt cgagccgatg gaacgccagc atcgacggcg tgatcgtgaa tgtcacgtcc
     6901 cgtgactcgc ccggcgcgag tgtcacgcgc cggaagccgc gcaattggcg caacggccgg
     6961 ctgacgtccg cgacctcgtc gcggatgtac aactgcgcga cttcggttcc cgcgcgcttg
     7021 ccggtattgg tgacgcgcac cgtcgcatgc agggtgtcgc cgggcgccag ggcgtggctg
     7081 tcgagcagca ccggtccgaa ggcgaacgtt gtgtagctca agccgtagcc gaacggatac
     7141 aacggcgtgt tcggaacatc gacgtaacgg ctggtgtacg ccggctgggc cggatcgaat
     7201 ggcctgccgg tctcgaggtg cgcgtaatgg atcggcacct ggcccaacct gcgcggaaag
     7261 gtaatcggca gtttcccgga aggattctcc tcgccgaaca ggaggtcggc gaccgcatgg
     7321 ccaccctcgt cacccggcag ccatgcttcc agcaccgcgt cggcgtgttc gtgcaaccac
     7381 ggaatcgtca gcggtcgtcc gttgaccagc acgaccacga tggggacccc ggcggccttc
     7441 accgccctgg cgagttcgag ttgccggccg gggagatcga tgtgcgcgcg gctgttgccc
     7501 tcgccgatca tggggcgatc ctcgccgagc accagcaccg cgatgccagc ggacctggcg
     7561 agtttcacgg caccctcgat tccggacatg tcgtcgccgt gaacgtcgac gcctttcgcg
     7621 aatgccacgt cgacatcgtt gccggcaact tcgcggatgc cgtcgaggat cgtcaccacg
     7681 tcgcgcggct tggcgaccgc aggcatcggc ccctgcagcg tttcgcgcgc cctggcgagc
     7741 gggccgatca ccgcgatgcg cccgcggcgc tgcagcggca aggtgccgtc gcggttttcg
     7801 agcagcacca tcgattcgcg cgcgatcctt cgcgcgagct cgcggtgccg cggcgacagc
     7861 gtcacgcgtt ccgcggttgc gcgattgccg taacggaatg gatcgtcgaa caaccccagc
     7921 ttgtacttcg ccaccagcac gcggcgcacc gccgcatcga gttcggcctc cggcaccctg
     7981 ccgctgcgca ccagcgcggg cagttccttc aggtaggtgc cgctgtgcag gtcgatgtcg
     8041 accccggcgt gcaacgcgag tcgagcggcc tcggctgggt cgcgggcgat cccgtgctgc
     8101 tgcaattcgg gcacggcgtc gtagtccgag acaaccagcc ccttgaagcc ccaatggtcg
     8161 cgcaggatac cggtcagcag ctcgcggtcg gcggtcgccg gcacgtagtc gagcgcgacg
     8221 aatgcggcca tcaccgatcg ggcgccggcg tccaccgcgg cacggtatgg cggaaggtac
     8281 acgtcgcgga attcgcgcgg cgggatccac gccgagttgt agtcgcgccc ggcctggacc
     8341 gcgccatagc cagcaaagtg cttgaccgtg gccatcatgg tgtccggggc ggcaaggtcc
     8401 tcgccctgga aaccgcggac ctgcgcggcc gccatcgcgc tgccgaggaa cggatcctcg
     8461 cccgcaccct ccaccaccct gccccagcgc gggtcgcgcg aaatatcgac catcgggcag
     8521 aacgtccagt cgacgccggc ggcggtcgcc tccgtggcgg ccgcgcgatc gacctgttgg
     8581 accagcgccg ggtcccaact ggcggccatc gccagcggca ccgggaacag cgtcgcgaac
     8641 ccgtggcaca cgtcccccat gaaccacagc gggatcttca agcgcgactg catcgcgagc
     8701 tgctgcatgc ggtgcgtgta agccacgaca tcggtgtcct gcagcacgcc attggtgccg
     8761 ccgaccttgc cctccgggac cagttgctcg aggtccttgt cgtcggcgcc gaggatcgtg
     8821 agttggccga ccttctcctc gatcgtcatc cgctgcagca ggccgtcgac gaacgactgc
     8881 atcgccttgt cgtcgtgccg caacggttgg gcatcggccg caagcgccga cgcggaaggc
     8941 gccaaccaca ggatcgccaa aacggcgaaa gcgatgcgga tcatgcgcag gatcccttgc
     9001 aggggtattc ccgcatccta acgcgcacgc acggacgaac ggtgcgacga gcgcgcgcat
     9061 cacgcgcccg cgggatggtg gttgggccat cagcttcaca aatgacctac ggtcatttag
     9121 agtttgcggc atccccctgt acccccggac ctcccgagga acacacatgt cccatcccgc
     9181 cgcgtcctcc aaggctgtac tcatcaccgg ctgctccacg ggcatcggca aagccaccgc
     9241 cgagcgcctc gcgcgcggcg gatggacggt gtacgcgact gcccgcaaac cggagacgct
     9301 ggaagatctg aaagcgaccg gctgccgcac gctcgcgttg gacgtccgcg acgaagggtc
     9361 catgcaggcg gcggtgcacg cggtggaaga agcccatggc gccgttggcg tgctgatcaa
     9421 caatgccggc tacagccagt ccggcgccgt ggaatcggtg cccctgtcga aggtgcgcgc
     9481 gcagttcgaa accaatgtct tcggcctggt ccggatgtgc caactggtac tcccgggcat
     9541 gcgccgccgc ggcttgggca ggatcgtcaa catgggttcc atggggggca agctcacatt
     9601 cccgggcggc ggcatctatc acgccaccaa gtacgccgtg gaagcgctca gcgacgcgct
     9661 gcgtttcgag gtgcgagggt tcggcgtgga cgtcgtgctg atccagcccg gcctgatccg
     9721 cagccggttc agcgccacgg tgggcgaggc catcgacgcc gtgcaagccg atgcgggtcc
     9781 gtacgaagcg ttcaacgccg ccgtagccaa gtccacccgg gacgcctacg aaaacggtct
     9841 gctggcaagg ctcggcggcg acccggaagc cgtcgcggcc gtgatcgaaa gggtcctgcg
     9901 cagcgcccgg cccagggcgc gctataccgt cacgccctcg gcccgactga tgatgacgca
     9961 gcgcgccttg atgagcgatc gcctgtggga ccgcttcctg cgcggcaact ttcccacccc
    10021 gggatccccg ggctgactgc cggcatgcac ggcgcccgcg cacgcaagca tccgccggcc
    10081 attcgccggc gggcggtcag cgacgaccag aaggcccgcc gccgccgcgc gatcctcgaa
    10141 gcggcgctgg agctgttcga cgcggttggt ttcgagcgcg tcagcatggc cgcggtggcc
    10201 gcgcgcatcc ggctggccaa gggcacgctg tatctctact ttcccacccg cgaggcgctg
    10261 ttcctggcgc tgttgaccga gcagttcgag gcgtttttcg acgcgctcga cgctgcgctg
    10321 gagcggcccc tctcgcggag cgcgttcgtg gaccacctgg tccgggactt gcgcgaccgg
    10381 ccacggctgc tgcgcctgat cgccgtgatg cacacggtgc tggagcggaa tgtcgatctc
    10441 gacacggcga tcggattcaa gcgcttcctg gccacgcacg cccaacacag cggtgcgcgg
    10501 atcgatgcca ggctccgcgg cgtgcccgcc ggcttcggcc tgcgcctgtt gctgtggctg
    10561 cacgccctgg cgatcgggtt gcaccacatg gccacgccgg cgccggtggt gcgccaggcc
    10621 atccaggcgg acccgcgcct cgcggtgttc gacattgatt tcgcgcggga actgcgcgcc
    10681 atgctcatcc gcctgttgca ggcgccggcg ggaagcacct tgggcctcga tgggtaatcc
    10741 tcaggccttg agccgccacc cggttcggaa aatccaccac acgcccgcga ggcacaacaa
    10801 caggaacccg agtgtcgcgg ccacgctgat cccgacgttg acgtcggcga ggccgtagaa
    10861 gctccagcgg aatccggaga ccaggtacac caccgggttc aacaaggtga ccttctgcca
    10921 cagcggcggc agcacatgga tcggatagaa gctgccgccg aggaacgtca acggggtgat
    10981 caccatcagc ggcaccacct gcagcttctc gaagttgtcg gcccagatcc cgatgatgaa
    11041 cccgaacagg ctgaaggtga cggtggtcat caccaggaac gcgaccatca ccagcggatg
    11101 cgcgatcgaa tagtcgacga acaaccgcgc ggtgccgagg atgatcagtc ccacgaggat
    11161 ggccttggtc gcggccgcgc ccacatagcc catgaccact tcgaggtagg acaacggcgc
    11221 cgaatgcacc tcgtagatcg tgcccacgaa tttcgggaag tagatcccga acgaggcgtt
    11281 ggaaacgctc tgcatcagca gcgacagcat caccagcccc gggacgagga aggccgcata
    11341 cgggacgccg tcgatgctgc tcatccgtga accgatcgcc gatccgaaca ccacgaagta
    11401 cagggacgtc gataccaccg gggacagcac gctctgcatc agcgtgcggc cggtgcgcgc
    11461 catctcgaag cggtagatcg cgcgaacgcc gtgcaggttc atgcgccctc cattatgcgc
    11521 accccagcgc atgaaatccg ccatccatgg ccgtactttt ccagaacagc agccactgaa
    11581 aatcgccacg aggaattcat gggccttccc tcactagatc cacgaagatc tgctccagcg
    11641 aatcctggcg ggtgctcaaa tccttgaatc cgataccggc ggccgagaga tccttcagca
    11701 gcgaggtgat gccggtccgc tcgcgccggg tgtcgtaggt atagacgagt tcgtcgcgcc
    11761 cgccgttgag gtccagcgca taggcggaca gcgactcggg caaggcgtcg atcggctcgt
    11821 tcaattgcag tcgcaactgc ttcttgccga gcttctgcat cagcgtggtc ttttcctcga
    11881 ccacgatcag ctcgcccttg ttgatcacgc ccacccggtc ggccatgtcc tcggcctcct
    11941 cgatgtaatg cgtggtgagg atgatcgtga cgcccgactc gcgcagccgc cgcaccatcg
    12001 cccacatgtc gcggcgcagc tcgacgtcca cgcccgcgct cggttcatcg aggaacagca
    12061 ccgtcggctg gtgcgacaag gccttggcga tcattacccg gcgcttcatg ccaccggaca
    12121 gcatcaggat cttgttgtcg cgtttttccc acagcgacag gtccttgagc aactgttcga
    12181 tgtatgcgtc gtcgggggcc ttgccgaaca agccccggct gaaactcacg gtgtcccaca
    12241 cggattcgaa cgcgtcggtg gtgagttcct gcggcaccag cccgatcatg gaacgggtct
    12301 tgcgccattc acggatgatg tcgtgcccgt ccacggtgat cgagccgtgc ccgggattga
    12361 cgatgccgca gatgatgctg atcagggtgg tcttgccggc gccgttcggc cccagcaacg
    12421 cgaagatctc gcggcggtgc accgtcaggt cgatgccctt gagggcctgg aagccgccct
    12481 tgtaggtctt gtcgaggccg cgcacctcga tcacgggatc agtcgccgcg cgcggcgggg
    12541 aaagggtttc gttcatgggc gtgccttcgg accgctgttt ggcgtcgctg cggccgtcat
    12601 cgaaccggcg ggcatcgtat tgacgatcgt gactggatcg caacccgcgt tgcggagtgc
    12661 aaggcaacga cgaacgaacg aggcgggatt cgacagcctg ccacggcgct ggtcgatgat
    12721 cagtgttcgg cttgacgcgt gtagatgcgg ccgtgtttca ccacgaagtc gatcttcgtg
    12781 gtgttgtgga tgtcctgcga cggatcggcg tcgagcacca ccaggtcggc gagcttgccg
    12841 ggcgtgaccg tgccccgctt cgcggactgg ccgagcgccc cggcaccgac ttcggtggcg
    12901 gcctcgatcg cctgcaacgg cgtgaagccc gcgtgctcga ccagcagcgc catctcctcg
    12961 tgcaccgaag gcggctggtc cagttcgggt ccgtccttgc cgtacgcgaa gcccgcgtcg
    13021 tcggtgcccg catcgaccag gacaccgcgc tggtgcgcca gcttcgtcac cgcgtagctc
    13081 cacggcatga tgcccccgcc gacacgttgc ggatggtgtt ccgcctcgtt cttgaacacc
    13141 agcagcgtcg cgtcgaggat cgtgccgcgc ttcttcatcg catcgaacaa cgcgaggatc
    13201 cgcggtgcag ccggtttgat gtgctcgaaa tcacccatcg cgcgcatcct gtaatcgtcc
    13261 ggcacgctcg gggccgcttc ccacaccagg taagcgctgt gcgagatcgt gtccacgccc
    13321 gcatcgaccg catcgctcgg ctttgccggg aaaatcgtcg cgtgcgtcca cacccgcaag
    13381 ccctgccggc gcgcctccgc agtgatcgcc tcgaccagcg ccgcgggcag gttggcgtac
    13441 agcttgatac cggtcgcgcc ggttcccttg gcttcggcga ccgcaagccg aacgtccgtc
    13501 ttggcggtga tcgcctgcat ccacggtgcg aagcccagcg gttcaccctt cgaggcatcc
    13561 tgcgcgcgga agtcttcctt gaagaaggtc ggtccggcca tcagcgcgac gtagaagatg
    13621 tccggcgaag cgaacgtgtc cgaattggcc tggtcggcga ggaactgcag cagccggtcg
    13681 tcgcccgcca tgtcgcggac gccggtaacg ccacccaaca gcaaggcgtg caggaacgtc
    13741 tggtagtgcg cgatgtccgg ttcggcatcg gtgatgtgca cgtgcgcgtc gatcaggccc
    13801 ggaatcacgt acttcccgga gaggtcgcgc acttcggcat ccttcggcgc cggcaggctg
    13861 tcgccgggat ataccgccgt gatttgctgc ccctcgatcg cgatggtcat gtcgcgttgc
    13921 accggcgaac cgctgccatc gatcagtccg acgtgggtca gcagcaacgg cttaggcgcg
    13981 acggccagcg ccggccacgc gaacaggcac aacaacagcg gcaggatgcg tttcatggaa
    14041 cccccgaatg acggccaagg ataaagggga tgcgcttggg cagccggacg attgcgcgct
    14101 cgatgggctt tttggccttg tcgacattga cgaaggcgtc cgggtcgagc gtagccaagt
    14161 cgcaacaccc aaagtccgct tcgaatggca attccaagtc gcgccattgg cgctacgctc
    14221 agtgcgaacg gcccattaca tgcaacgtct ttgcaggtcg ggttgatccc ggatctggtc
    14281 cgggagaagc ccaacgttga gccaacgggt tacagtagtc ggcgaacgaa aaggagaccg
    14341 ccatggacgc gaacacggac cgcatcgaga agcaggtcct gctgaaggtg ccgcgcgagc
    14401 ggatgtggcg cgcgctcgcc gacgcggatc agttcggcac ctggttcggc gttcgcttcg
    14461 atgcgccgtt cgtcgaagg
//
LOCUS       DASSNH010000229         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1147094, whole genome shotgun sequence.
ACCESSION   DASSNH010000229 DASSNH010000000
VERSION     DASSNH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147094"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2202)
                     /locus_tag="VFK00_08325"
     CDS             complement(<1..2202)
                     /locus_tag="VFK00_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET7592992.1"
                     /translation="MLQDEPPLARIEVAPVLLETVAKGRLGRAVPLDPKQLLHAALSE
                     ADARLAASILGLPQTRRKGRSYAQLAGAFGDALLREILTSAPSLLGGVAGLRLALGKP
                     RPLEWDWQLERDGRQRLLPKLPAHQRVVRVGGLWYLDPERAEFGTLEAGPAEVALIDA
                     PPLAPEYAEALAARMAASAWAARVPVPQAFDAVQRSDVSPKPVMIFRALTRHPRIGAG
                     TPPLAYARLAFDYGGERLPGRGGEATVRRVRNGKLVEIMRKRAEELATMERMEAFGLA
                     PAVDCEGLPWDMADALPEDAWVFPGKGYAGALEVNTPARWLGVREKLEHDGFVVEYDS
                     TFPFEVLEGPPRWYGVARADDGGEAFDFEMGIEVEGKRVNLLPTVARALSEHTLSLTA
                     PENETNDAVWYAPVDERRRVAVPLAVLRKLLAPLAEYLAHPRDKLRLPRVQAGRLEEF
                     AGALPKGGKLDAPKDLTGFTTRLREASEHASDAVPEGLAGELRHYQREGLRWLNALAA
                     AGLGGVLADDMGLGKTVQMLAHILVLKQNSALTQPALIVAPTSLIPNWQAETARFAPS
                     LKVLTLHGPERVKSFDRLAGHDVILTSYALLPRDLQVLREQPFALVVLDEAQQVKNPR
                     TQARRALLSIASKRRVCMTGTPLENHLGELWSQVDLAVPGLLGDEGSFRRHYRLPIER
                     QADADCQARLNHRIAPFILRRTKAQVVSELPEKTEIARRVVLSGKQRELYDS"
     gene            complement(2549..3568)
                     /locus_tag="VFK00_08330"
     CDS             complement(2549..3568)
                     /locus_tag="VFK00_08330"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7592993.1"
                     /translation="MAARATVAVLGAGSWGTALAALLCGNGIATRLWGRDAAALAGIA
                     ASRRNRRYLPDLELPAELACVPDLADALEAVDLALVVVPSHGFHELLDEAVPSLPAGA
                     GFAWATKGFEPGTGRFLHELVAERLPDTPAAVVTGPSFAREVAAGMPTAVTVHSGDDA
                     FAHRVAELLHNHTFRAYTGNDMLGAELGGAMKNVLAVATGVADGMELGLNARAGLITR
                     GMNEMLRLGAALGAQPQTLMGLAGLGDLVLTCTGQLSRNYRFGQALGRGVSLDEALAD
                     IGQVVEGTVAADEVMRLAQRHDLDLPISQHVQSVLRGEMTPTDALHSLLARERKPEYP
                     ETLFG"
     gene            complement(3572..>4052)
                     /gene="secB"
                     /locus_tag="VFK00_08335"
     CDS             complement(3572..>4052)
                     /gene="secB"
                     /locus_tag="VFK00_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET7592994.1"
                     /translation="QAAPQMQMALQKIYARDVSFEVPNAPKVFQEEGQPQVQLNLSHK
                     ANALEGGNYEVVLTLTVTCTLNEHTAYLAEVHQAGVFSFVGFDPQNLTTVLATYCPNV
                     LFPFARQTISDLVLNGGFPPLLLQPINFDALYAQQQQQQAQQQGQLGNGGDKPTLNA"
BASE COUNT          608 a         1426 c         1389 g          629 t
ORIGIN      
        1 cgagtcgtac agctcccgct gcttgcccga cagcaccacc cgcctcgcga tctcggtctt
       61 ctcgggaagt tcggagacca cctgcgcctt ggtgcgccgc aggatgaagg gcgcgatccg
      121 gtggttgagg cgcgcctggc aatcggcgtc ggcctggcgt tcgatcggga gccggtagtg
      181 gcggcggaac gatccctcgt cgccgagcag gcccggcacc gcgagatcga cctgcgacca
      241 gagttccccc aagtggtttt cgagcggggt gccggtcatg caaacgcgcc gcttcgatgc
      301 gatcgacagc agcgcgcgcc gtgcctgggt gcgtgggttc ttgacctgct gcgcctcgtc
      361 cagcaccacc agcgcaaacg gctgctcgcg cagcacctgc aggtcacggg gcagcaatgc
      421 gtagctcgtg aggatcacgt cgtgcccggc gagccggtcg aaactcttga cgcgctccgg
      481 gccgtgcagg gtcagcacct tgagcgatgg cgcgaagcgt gcggtttcgg cctgccaatt
      541 cggaatcagg ctggtgggcg cgacgatcaa cgccggctgc gtcagcgcag agttctgctt
      601 gagcacgagg atgtgggcga gcatctgcac ggtcttgccg aggcccatgt cgtcggcgag
      661 cacgccgcca aggcccgccg cggcgagtgc gttcaaccag cgcaacccct cccgctggta
      721 gtggcgcagt tcgccggcca atccttccgg cacggcgtcc gatgcgtgct ccgatgcctc
      781 gcgcaggcgc gtcgtgaacc cggtcaggtc cttcggtgca tcgagtttgc cgcccttcgg
      841 caatgcaccc gcgaattcct ccagccggcc ggcctgcacc cgcggcaggc gcagcttgtc
      901 gcgcgggtgc gcgaggtatt cggcaagtgg cgcgagcagc ttgcggagca ccgccagcgg
      961 caccgccacc ctgcggcgct cgtcgaccgg cgcataccac accgcgtcat tcgtctcgtt
     1021 ctccggcgcg gtcagcgaca acgtgtgctc ggacaacgcg cgcgcgaccg tcggcagcag
     1081 gttgacgcgc ttgccttcga cctcgatgcc catttcgaaa tcgaaggcct cgcctccgtc
     1141 gtccgcgcgc gcgacgccat accagcgcgg cggtccttcc agcacctcga acggaaacgt
     1201 cgagtcgtac tcgaccacga atccgtcgtg ctccagcttc tcgcgcacgc ccagccaccg
     1261 tgccggcgtg ttgacctcca gtgccccggc gtagcccttg ccgggaaaca cccaagcgtc
     1321 ctccggcagc gcgtcggcca tgtcccacgg caggccctcg cagtccacgg ccggcgcaag
     1381 cccgaacgcc tccatccgtt ccatcgtggc cagttcttcg gcacgcttgc gcatgatctc
     1441 gaccagcttg ccgttccgca cgcgccgcac ggtcgcctcg ccgccgcgac cgggcagccg
     1501 ctcgccgccg tagtcgaatg ccagccgcgc ataggcgagc ggcggggtcc ccgcgccgat
     1561 gcgcggatgc cgggtgagcg cacggaagat catcaccggc ttgggcgaaa cgtccgagcg
     1621 ctgcacggca tcgaatgcct gcggcaccgg cacgcgcgcc gcccatgccg atgcggccat
     1681 gcgcgcggcc agtgcttcgg catattcggg ggcgagcggc ggggcgtcga tcaacgccac
     1741 ttccgcaggg ccggcctcca gcgtgccgaa ctccgcgcgc tccggatcga ggtaccacaa
     1801 gccgccgacg cgaaccacgc gctggtgggc cggcagcttc ggcaacagtc gctggcggcc
     1861 gtcgcgctcg agttgccagt cccattccag cgggcgcggc ttgcccaacg ccagccgcaa
     1921 gcccgcgacc ccgcccagca ggctcggggc cgaggtcagg atctcgcgca gcagtgcgtc
     1981 gccgaaggcg ccggcgagct gcgcatagct gcggcccttg cgccgggtct gcggcaagcc
     2041 caggatgctg gccgccagcc tcgcatcggc ttcggacaac gccgcgtgca gcaattgctt
     2101 gggatcgagc ggcaccgcgc ggccgagccg acccttggcc acggtttcca gcaacacggg
     2161 agcgacctcg atgcgcgcca gcggcggctc gtcctgcaac atttccagca ggaagccgag
     2221 ggtggtcgca tcggtattgg ccgcggggtc atccggggcc ctggccagca gccgtgtcca
     2281 ttccgagggc aggggcgggg tccgcgcggc gttgcgcggc acggatgtct cctgctggtc
     2341 gctgcggtgt tgcatgcgcg cggggcactc aaaaggctca agcatacacg tacttttgcg
     2401 atcatccatg atcgctgccc cggccagcca cgaggtcgcc gcgccgcatt cacccaacgg
     2461 acaccagaac gggcatccct gccctgactt tccgccaaaa gcggcttcga gcgcgcaccg
     2521 ggtggggccg cgaaagcctc gctacctttc agccgaacag ggtttcggga tactcgggct
     2581 tgcgctcgcg ggcgaggagc gaatgcaggg cgtcggtggg ggtcatttca ccccgcagga
     2641 cggactggac gtgctgcgag atcggcaggt cgaggtcgtg gcgttgggcg aggcgcatca
     2701 cttcgtcggc cgccacggtg ccctcgacca cctggccgat gtcggccagc gcctcgtcga
     2761 gcgacacgcc gcggccgagc gcctgcccga accggtagtt gcgggacagt tggccggtgc
     2821 aggtgagcac caggtcgcca agccccgcga ggcccatcag ggtttgcggc tgcgcgccaa
     2881 gcgccgcgcc cagcctgagc atctcgttca tgccgcgggt gatgagtccc gcccgggcgt
     2941 tcaggccgag ctccatgccg tcggccacgc cggtcgcgac cgccaacacg ttcttcatgg
     3001 cgccgccgag ttccgctccc agcatgtcgt tgccggtgta ggcgcggaag gtgtggttgt
     3061 gcagcagctc ggccacccgg tgcgcgaagg catcgtcgcc ggagtggacc gtcaccgcgg
     3121 tgggcatccc ggctgcgacc tcgcgcgcga acgaaggacc agtgacgacc gccgccgggg
     3181 tgtccggcag gcgctcggcc accagttcgt ggaggaaacg ccccgttccc ggctcgaagc
     3241 ccttggttgc ccaggcaaag cccgcccccg cagggagcga gggcacggct tcatccagta
     3301 gttcgtggaa cccgtggctg gggaccacca ccagcgccag atcgaccgcc tccagcgcat
     3361 cggcgaggtc cggcacgcaa gccaattcgg ccggcagctc caggtcgggc aggtagcgcc
     3421 ggttccggcg gctggccgcg atcccggcga gcgccgcggc gtcgcggccc cacaagcggg
     3481 tcgcgatgcc attgccgcac aacaaggcgg ccagtgcggt gccccacgag ccggcgccga
     3541 ggacggcaac cgtcgcgcgc gcagccatgg ttcaggcgtt cagcgtcggc ttgtcgccgc
     3601 cattgccgag ctgcccctgt tgctgcgcct gctgctgctg ttgctgggca tacagcgcgt
     3661 cgaaattgat cggctggagc agcagcgggg ggaatccgcc gttcaggacc aggtccgaaa
     3721 tggtctgccg cgcgaacggg aacagcacgt tcgggcagta ggtcgccagc accgtcgtga
     3781 ggttctgcgg atcgaagccg acgaacgaga acacgccggc ctggtggact tcggcgaggt
     3841 aggcggtatg ctcgttcagg gtgcaggtca ccgtgagggt caataccacc tcgtagttgc
     3901 cgccttccag cgcattggcc ttgtggctca ggttgagctg gacctgcggc tggccttcct
     3961 cctggaacac cttgggcgca ttcggaactt cgaacgagac gtcgcgcgcg tagatcttct
     4021 gcagcgccat ctgcatctgg ggggcggctt gg
//
LOCUS       DASSNH010000230         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_692973, whole genome shotgun sequence.
ACCESSION   DASSNH010000230 DASSNH010000000
VERSION     DASSNH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692973"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFK00_08340"
     CDS             complement(<1..583)
                     /locus_tag="VFK00_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7592995.1"
                     /translation="MQASPARGRAIEILAYRKLRLDDYPDLADLLRSFVAADVGAPVR
                     RCVIACAGQVMGDKVIHDNLAWPIKLSRLRKALGFDDVAVLNDFEALGYALDEIRSAG
                     GQLLCGPDTRAKGPTLVIGPGTGLGAAVRLAGRERQVVLTTEAGQMDFAPQTIREREV
                     LRSVSPGGGYVPYESIVSGPGLLTLYRTLCGLNG"
     gene            complement(940..3687)
                     /locus_tag="VFK00_08345"
     CDS             complement(940..3687)
                     /locus_tag="VFK00_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7592996.1"
                     /translation="MDSTEHPDRPLYRYRFDDAEFDEARVTLRVAGERVDLEQRPLQV
                     LALLLQRVDEVVPRAELFDTVWAGRPTVDNVLANAVAKLRKALGATAGARIVNVPRVG
                     YRLRGPVERMVAGRRASGGFELAAGQAVPGREHFRLEACLDASGGAAVWLARHEKTGE
                     PRVYKFAVDGERLAALKREVTIYRVLKECLGERDDRVRLFDWNFDTPPFFLECEYAGQ
                     DLARWSQENPAFDIAPRERRIAWFIEIAEAVAAAHGAGVLHKDLKPANVLVMPRGDGW
                     RWRVTDFGSGRLLDPGKLESLGITRLGMTVTGALSEQSGVTLLYLAPELLAGAAPSVR
                     SDVYALGMMLFQMVVGDLHRTLAPGWEQAFETQDIDDQLLCEDIAAATSGDPERRLAS
                     VDALVQRLRSREARAIERKRLRDSDARALAAERLLERSRARRPWLIAAVVLLGAGLGV
                     SLWQLRQIRTARTEAQQQAALAAATNRFLNNDLLGAGVGGNSPAWYERNPTLREILDT
                     AAKHLDDGRFAGEPLVLADLHQTLGRAYRSTGDFAKAGGQLQAAADWLQRVLGANGQR
                     TVLAQYELAGVLSHLSRFKDADALLDRADAAAGARRDAVSEIALRAHLARGDVLYQQM
                     RVEPALAAYIVAERLQRQLHPDDVMLSMHLLLAIAGCDLRMGKPKEAESVARKILSGA
                     PYTQERIGLAAIATAHSRLGDALRGQGQYAEAIPVTQQSLQEYETSQGQDSQGAISAL
                     STLSYLYSLTGDDAKALTLQRDVYQRAVKRWGANNQYALVELLNLGSSEYDSGDLKTA
                     LPHLQQAEGGLVKVSGEKSPTTQAARVALANALADLGRNEQALAMIASVDPVAYQATT
                     SDPDRAVVLKAMQARIELRLHNPGSAARLRAAIAAMQTAGVADDEIATFRSALADADV
                     H"
     gene            3902..4204
                     /locus_tag="VFK00_08350"
     CDS             3902..4204
                     /locus_tag="VFK00_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592997.1"
                     /translation="MIGAWLMLLCVAAVLFLIAESWRAWAWHHRGVGERKALVEYARI
                     RREQPDTAEARLPEAEFVRYYVGLRPGIARYAIVALLLVLIGLPASCALMAGWRWD"
     gene            4229..4744
                     /locus_tag="VFK00_08355"
     CDS             4229..4744
                     /locus_tag="VFK00_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592998.1"
                     /translation="MTTRHRHVWLHVALLALAAGFGTGSARALTLHQLKGAQLDSIYG
                     TYAPRSDCQREPRITVDDSGFTYHYRGHTSHPTTFEYALTYMGQDYQGIGQWFFPFPV
                     NESDFGRVLMTINPDEKAGTLKFEPNVAPGESLTPLQAALVKGSPYAKCAKPIATSGV
                     APRAARQGAVP"
     gene            4741..6165
                     /locus_tag="VFK00_08360"
     CDS             4741..6165
                     /locus_tag="VFK00_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7592999.1"
                     /translation="MKATHTTCRHFRRSVSTVLLLVLVAISFNAAAACSGKLANLQSY
                     AGNYRATSDLLAAPELATLLQRLPGDEATHLTRNLDVSGPVMLVNCHLVVSGNAPHMG
                     TEQDAMLDVDLVSGTVIAAIHGGGRIDIYTLADPASGAPGWNALPRSMREWAVRADMG
                     FPQQPPHSLAQPDSVRLHAPPPVAATANAAPPSTGKPLQINFDKDAIKPNAAQAAAIR
                     RAAGDALAQPLPGHAGEPLYAVALADLNDDGRADLVVQYTYASGFCGSAGCSGMILMA
                     TPRGHPGKGIGLPNFGTLAVLPATHGGMHDLQFNGDSPIWRWTGKDYEIPKADLPQSN
                     AEPWKTAQAPGHPLMAYVTPIDSVIKRVFVACEQGRPLLLILTKQALPAGAKTLTFVF
                     NGWTVNVPLQRNVTNEDLWMADLPRSQLPYWFAHRGFDANTRQIAPHVTQSFLRINGE
                     MQGQISMTNSTAASQAALSSCYRY"
     gene            6213..>6533
                     /locus_tag="VFK00_08365"
     CDS             6213..>6533
                     /locus_tag="VFK00_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593000.1"
                     /translation="MRGKIIQYNGADGSGTIVADGRQYCFALASWRGDSAPSINKTVE
                     FVADGDAVTAVTVVGDDVLIKEKAAELGGKLGASLNKLRASIPASGGAAAGTPTGGAT
                     PVAGG"
BASE COUNT         1107 a         2317 c         2062 g         1047 t
ORIGIN      
        1 ccccgttcaa cccgcacagc gtccggtaca aggtcagcaa cccggggccg gacacgatgg
       61 actcgtacgg cacgtacccg ccgccgggcg acacgctccg taacacctcg cgctcgcgaa
      121 tggtttgcgg cgcgaagtcc atctgccccg cttccgtcgt caacacgact tgccgctcgc
      181 ggccggcaag tcgcaccgcc gcgccgagcc ccgtccccgg accgatcacc agcgtcggtc
      241 ctttcgcccg cgtgtcgggg ccgcacaaca actggccgcc cgcgctgcga atctcgtcaa
      301 gtgcgtagcc gagcgcctcg aagtcgttca gaacggcaac gtcgtcgaat cccagggcct
      361 tgcgcaggcg cgagagcttg atcggccatg cgaggttgtc gtgaatgacc ttgtcgccca
      421 tcacctggcc agcgcaggcg atcacgcaac gacgcacggg cgcgcctacg tcggcagcca
      481 cgaacgaccg cagcaaatcg gcaagatccg gatagtcgtc caggcgcagt ttgcgatagg
      541 caaggatttc gatggcgcga ccgcgcgcgg gggacgcctg cacccatgcg acccgtgcgt
      601 gcgttccgcc gacatccgca gctagaaatg gccgcgccga cacgcgctcc tgccgattcg
      661 aacgattgac cacagcgccc ttcaacccca cgcatggcga accctctcca gtgtgggcag
      721 cgatgacacc ggtgtcaatt tgcaccgcaa caaggtctcg cccggctcgc ctggggccaa
      781 ttttcctgcc gttcaccctt ccgcacgaga aaaagcagcg ggcgagcaga cccccgcggc
      841 aggcaaaaat gctgcgtcac agcaccgttg ctgcgcaagc gtctcgcttt cgcgtcgtcg
      901 gcgcggtccg ttccatcccg gttcaagccg tggtgctgct cagtgcacgt ccgcgtcggc
      961 cagcgccgag cggaacgtcg cgatttcgtc gtccgcgacc cctgccgtct gcatggcagc
     1021 gatggcagcg cgaagccgcg cggccgaccc cgggttatgc aagcgcaatt cgattcgtgc
     1081 ctgcattgcc ttcaggacaa cggcacgatc cggatcggag gtggtcgcct gataggccac
     1141 cggatcgacc gaggcgatca tcgccagtgc ctgctcgttg cgtccgaggt cggccagggc
     1201 attggccaag gcgacgcgcg cggcctgagt cgtcggactc ttctcgccgc tgaccttcac
     1261 gagtccgcct tcggcttgct gcaagtgcgg caatgcggtc ttcaggtcgc ccgagtcgta
     1321 ttcgctggac ccgaggttca acagctcgac cagtgcgtac tggttgttcg cgccccagcg
     1381 cttgaccgca cgttgataga cgtcgcgttg caaggtcaac gccttggcat cgtcgccggt
     1441 cagtgaatac agatagctca gcgtgctcaa cgcactgatg gcgccctggc tgtcctgccc
     1501 ctgcgaggtt tcgtattcct gcagcgattg ttgcgtgacc ggaatcgcct ccgcatactg
     1561 gccctgtcca cgcaacgcat cgcccagccg cgaatgtgcg gtggcgatcg cggccagccc
     1621 gatgcgctcc tgcgtgtagg gcgcgcccga aagaattttc cgcgcaaccg attcggcctc
     1681 tttcggtttg cccatccgca aatcgcaacc ggcgatcgcc agcagcaggt gcatggacag
     1741 catcacgtcg tcaggatgca actgtcgttg cagccgttcg gcgacgatgt acgcagccag
     1801 cgccggttcc acgcgcatct gctggtacag cacgtcgcca cgcgcgaggt gcgcgcgcag
     1861 ggcgatttcc gaaaccgcat cgcggcgcgc tccggctgcc gcatcggcgc gatcgagcag
     1921 cgcatcggca tccttgaacc gcgacaggtg ggagagcacg ccggcgagtt catattgcgc
     1981 cagtacggtg cgttgaccgt tcgcgcccag gacacgctgc agccagtccg ccgcggcctg
     2041 caattgcccc cccgccttgg caaagtcacc ggtgctgcga tacgcgcgtc ccagtgtctg
     2101 atggagatcg gccagtacca gcggctcgcc ggcgaagcgg ccgtcgtcga ggtgtttcgc
     2161 ggcggtgtcc aggatttcgc gcagggtcgg attgcgctcg taccacgccg gcgaattgcc
     2221 gccgacgcct gcaccaagca ggtcgttgtt gaggaagcgg ttggtggcgg cggccagcgc
     2281 ggcctgctgc tgcgcttcgg tgcgcgcggt cctgatctgc ctgagctgcc acaggctcac
     2341 gccgaggcct gcccccaaca ggacgaccgc cgcgatcagc cagggacgcc gcgcacggct
     2401 gcgttcgagc aggcgctccg ccgccagagc gcgcgcatcg gaatcgcgca gacgcttgcg
     2461 ttcgatcgcg cgcgcctcgc ggctgcgcag gcgctgtacc agcgcgtcca cgctcgcgag
     2521 gcggcgttcc gggtcgccgc tggtcgccgc ggcgatgtcc tcgcacagca actggtcgtc
     2581 gatgtcctgg gtttcgaaag cttgttccca gccgggcgcg agcgtgcggt gcaggtcacc
     2641 gacgaccatc tggaacagca tcatgcccag ggcgtacacg tcgctgcgca ccgatggcgc
     2701 ggcgcccgcc agcaattccg gcgcgagata cagcagcgtc acgccggact gctccgacaa
     2761 cgcgccagtg acggtcatgc ccagccgggt gatgcccaac gattccagct tgccgggatc
     2821 gagcaaccgc ccgctgccga aatccgtgac acgccagcgc cagccgtcgc ctcgcggcat
     2881 caccagcacg ttggcgggct tcaggtcctt gtgcagcacg ccggcgccgt gcgcggcggc
     2941 gaccgcctcg gcaatctcga tgaaccacgc gatgcgacgt tcccgtggcg cgatatcgaa
     3001 ggccgggttc tcctgcgacc agcgcgcgag gtcttgcccc gcgtactcgc attccaggaa
     3061 aaacggcggc gtatcgaaat tccagtcgaa cagccgcacc cggtcatcgc gctccccgag
     3121 acactccttg agtacgcggt agatcgtgac ttcccgtttc agcgcggcca gccgttcgcc
     3181 atcgaccgcg aacttgtaga cgcgcggttc tccggtcttc tcgtgacgcg ccagccatac
     3241 cgccgcaccg ccggacgcgt ccagacaggc ttccaaccgg aagtgttccc ggcccggcac
     3301 ggcttgcccg gcagccagct cgaacccacc gctggcgcgg cgtccggcca ccatgcgttc
     3361 gaccggcccg cgcagccggt agccgacgcg cggcacattg acgatgcgtg cgccggctgt
     3421 cgcgcccaag gccttgcgca gcttggccac cgcattcgcc agcacgttgt ccacggtggg
     3481 ccggcctgcc cacacggtgt cgaacaactc ggcgcgcggc accacctcgt cgacgcgctg
     3541 cagcaacagc gcgagcacct gcaggggccg ctgttccaga tccacgcgct cgccggccac
     3601 gcgcagggtc acgcgcgcct cgtcgaattc ggcgtcgtcg aaacggtaac ggtacagcgg
     3661 ccgatcggga tgctccgtgc tgtccacgtg tgcctacccc atttcgccct ctttggcgat
     3721 tgtgcaccaa ccacgcgcca gccgcacgcg gcctcgcgcg ccccgaaccg gcgccgttag
     3781 cgatccgtta gaccaccgtg gcgacatttt cgaagcccgg gcgcccaatc gcaccacggc
     3841 cgtccattgg ccactaccgc aaccggagac ctttccgcga tgacccgagg gatgaccgga
     3901 catgatcggt gcatggctga tgctgttgtg cgtggccgcc gttttgttcc tgatcgcgga
     3961 gagctggcga gcctgggcgt ggcatcaccg cggcgtgggc gagcgcaagg cgctcgtcga
     4021 atatgcgcgg atccggcgcg agcaaccgga caccgccgaa gcacgtctgc ccgaagcgga
     4081 attcgtccgc tactacgtag gtttgcgccc gggcatcgcc cgctacgcca tcgtcgcgct
     4141 gctactggtc ctgatcggcc tgccggcgtc ctgcgcgctg atggcgggct ggcggtggga
     4201 ttgaaccgtt tcacgggagg atccagacat gaccacacgc caccgccacg tatggctgca
     4261 cgttgcgcta ctggcactcg ccgccggctt tggaaccgga agcgcgcggg ccctgacgct
     4321 gcatcagctc aagggcgctc aactcgacag tatctacggc acctatgcac cgcgcagcga
     4381 ctgccagcgc gagccgcgga tcactgtgga cgattccggt ttcacctacc actaccgcgg
     4441 ccacacgagc cacccgacca cgtttgaata cgccctcacc tacatgggtc aggactacca
     4501 gggcatcggc cagtggttct ttccgtttcc cgtcaacgag agcgacttcg gccgggtgtt
     4561 gatgacgatc aatccggacg agaaggccgg cacgctgaag ttcgagccga atgtcgcccc
     4621 gggtgaatcg ctgacgccat tgcaagccgc gctggtcaag ggttcgccgt acgcgaaatg
     4681 cgccaaaccg atcgcgacat ccggcgttgc gccccgcgcc gccagacaag gcgccgtgcc
     4741 gtgaaagcca cgcataccac ttgccgtcat ttccgccgca gcgtatccac cgtgctgctg
     4801 ctggtgctcg tcgcgatttc gttcaatgcc gcggcagcgt gcagcggaaa acttgcaaac
     4861 ctgcagtcgt atgccggcaa ctaccgcgcc acctccgatc tgctcgcggc acccgagctg
     4921 gcaacgctgc tgcagcgcct gccgggcgac gaggcgacgc acctcacgcg caacctcgac
     4981 gtgtcgggcc cggtcatgct cgtgaattgc catctcgtcg tatccggcaa cgcgccgcac
     5041 atgggcaccg agcaggatgc gatgctggac gttgatctcg tgtcgggcac cgtgatcgcg
     5101 gcgatccacg gcggtgggcg catcgacatc tacacgctcg cggacccggc ctcgggcgcg
     5161 ccgggctgga acgcgttgcc caggtcgatg cgggaatggg cggtccgcgc ggacatggga
     5221 tttccgcagc agccaccgca cagcctcgcg cagccggaca gcgtgcgcct gcatgcgccg
     5281 ccgcccgttg ccgcaacagc caacgccgcg ccaccatcga cggggaagcc gctgcagatc
     5341 aacttcgaca aggacgccat caagccgaac gccgctcaag ccgccgccat ccggcgtgcg
     5401 gccggcgacg cgctggcgca accgctgccg gggcatgccg gcgagccgct gtacgcggtc
     5461 gcgctggccg acctcaatga cgacggtcgc gccgacctcg tcgtccagta cacctacgcg
     5521 tccggctttt gcggctcggc gggctgcagc ggcatgatcc tgatggcgac gccgcgtggc
     5581 catcccggca agggcatcgg tctcccgaac ttcggcacgc tcgcggtgct gcccgcgacg
     5641 cacggcggca tgcacgacct gcagttcaac ggggacagtc ccatctggcg gtggaccggc
     5701 aaggactacg aaattccgaa agccgacttg ccgcagtcca acgcggaacc ctggaagacg
     5761 gcgcaggcgc cgggccatcc gctgatggcg tacgtgacgc cgatcgactc cgtgatcaag
     5821 cgcgtgttcg tggcgtgcga acaaggccgg cctctgttgc tgatcctgac caagcaagcg
     5881 ctgccggcgg gcgccaagac gctgaccttc gtgttcaacg gctggacggt gaacgtgccg
     5941 ctgcaacgca acgtcacgaa cgaggacctg tggatggccg acctgccgcg ttcgcagttg
     6001 ccgtactggt tcgcgcaccg cggcttcgac gccaacacgc gccagatcgc cccacacgtc
     6061 acccagtcct tcctgcgcat caacggcgaa atgcaggggc aaatatccat gaccaactcc
     6121 accgcggcat cccaagccgc gctctccagt tgctaccgct actgacggac acgccccacc
     6181 gcttcgaccg accacagcag gaggaacaca ccatgcgcgg caagatcatc cagtacaacg
     6241 gcgccgacgg cagcggcacg atcgtcgccg acggcaggca atattgcttc gcacttgcca
     6301 gttggcgagg cgacagcgcc ccaagcatca acaaaaccgt cgagttcgtg gccgatggcg
     6361 atgccgtcac ggcggtcacc gtggtcggcg atgacgtgct gatcaaggag aaggcagccg
     6421 aacttggcgg aaagctcggc gcgtcattga acaaactgcg cgcgtcgatc ccggcttccg
     6481 gcggagctgc ggcgggtacg ccaaccggtg gcgccacgcc cgtcgccggt ggg
//
LOCUS       DASSNH010000231         6440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1272129, whole genome shotgun sequence.
ACCESSION   DASSNH010000231 DASSNH010000000
VERSION     DASSNH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272129"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFK00_08370"
     CDS             <1..544
                     /locus_tag="VFK00_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7593001.1"
                     /translation="MLIAALALAFAIAVAWLFNRLVRLRNQVRVAWSDVDVQLQRRHD
                     LVPPLVQTVKAYAAHESGLLERVAVERHAALSATSTPDRETSEAALGRSLTRLIAVAE
                     AYPDLKANGNFMQLSRQLVDTENLIQHARRFYNGSVRQYNTAIERFPAVLAARPLGFK
                     PAAFFAADDDARGNVQVGLG"
     gene            541..2424
                     /locus_tag="VFK00_08375"
     CDS             541..2424
                     /locus_tag="VFK00_08375"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7593002.1"
                     /translation="MRKRMRHHVLLMAFCLFGLGVGVVDAGAAERILDYHSDITLAVD
                     GGMQVTETIRVQAEGARIRHGIYRDFPTDYRDRQHHRYHVGFAVTRATRDGAPESWRT
                     ERQSNGVRVYLGDEATLVTPGVHTWTIRYRTNRQIGFFPDHDELFWNVNGTGWIFPID
                     RASASVHLPLAVPAAKLEAYGYTGSQDSREEALAASVHDGGADYITTRPLGSNEGLSI
                     VLEFPKGVIIAPSTAQRWRWWLADNSNILLAVIALVGLWIYYVQVWRRKGRDPAAGPL
                     VAEYEPPDGDSAAALRYVERMGYDDTCFTAGILGVAAKGGLDIREDDAKTYVATRRDG
                     GKAVFSADEKALRDALFAGGADIEFKQGEHSRVQAARSAHQKALKAAFYSKYFLTNSL
                     WLLPGVAITVVSAWLASLGGAEGTGFLLAWIGFWTVGVVLLFNRAAHGGRAAVGGWIM
                     FVFFAAAELIAIAALGTLAGYALIPLCIALVVTNLMFYQWLKAPTQLGAKLLDRIRGF
                     RWYLGVAEKQELDARYKPESRPDLFAAYLPYALALGVGNAWAERFSSALSPVQMEQAR
                     PSWYSGTGASGFNGASFTTFASSMTSGFGGAIASASMAPGSSSGSGGSSGGGGGGGGG
                     GGW"
     gene            complement(2564..3373)
                     /locus_tag="VFK00_08380"
     CDS             complement(2564..3373)
                     /locus_tag="VFK00_08380"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7593003.1"
                     /translation="MQKLDVYAPKGVHGAPIVFMVHGGGWWRGDKAFPGVVQNKINHF
                     LPKGIIFVSVNYRLSPQVGVLDQAKDVARALIYVQAHATQWGGDPNRVVVMGHSAGAH
                     LTSLMTTAADIHKGAKPWLGNIELDSAALNVVQIMKAPHPHSLYDPIFGADPNLWAEA
                     SPTLRITAAPPPMLMVCSIAHPGSCRQTEEFVDAAYGKAVEYQTGLDHGSIDTTLGLS
                     NQLTDAVDSFLCALGVGCGRTQAKGTASKTKTPPRVLCSLRKNSAKPCPKR"
     gene            complement(3534..3845)
                     /locus_tag="VFK00_08385"
     CDS             complement(3534..3845)
                     /locus_tag="VFK00_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593004.1"
                     /translation="MAPDDMLLANLAVKPPLPVEECHRSCAFSICADSAYQNRSGAAV
                     SCGQVQPHHGRCTEFDAPSELRDRNDIEATVTFTSFPRGDTRFATAFLLSRPNHPSSE
                     E"
     gene            4017..6146
                     /locus_tag="VFK00_08390"
     CDS             4017..6146
                     /locus_tag="VFK00_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7593005.1"
                     /translation="MKAIWPALALGVIVSGTALANPSPKNHEPASTASSGDPYLWLEP
                     SHSQRAMEWVHAQNAITQKAFMESPEFDKTRGKILEVLDSEARIPYVYRMGDYLYNFW
                     QDKANPRGIWRRTTLAEYEKADPKWETVLDIDALNKAEDKKWVFKGARCMKPDYTHCL
                     VSLSPGGGDAVEVREFDLPSKTFVNGGFFLPVAKSDVDWIDADHIYVGTDFGPGSMTK
                     SSYPRIVKEWTRGTPLSAATVVYEGKPTDLAIGAYHDRTKGYERDFVSVQKDFFHGDM
                     YLRDGGKLAKVDVPEDANADVHRQWLLVQTKSPWTTGGKTWPAGALIAMKFEDFMAGK
                     RDFTALFTPDAHTALAGYSWTQNHIILDVLDDVKSRLDVLTPPASGNGAWQHQALPGA
                     PKFSTIAVVDTDPDHSDEYWLTVTGFLDPPSLERGVLGREPAKKIKQEPAFFDAAKFE
                     VSQHFVTSKDGTRVPYFQIAPKDMKLDGSNRTLEYGHGGFEVSLLPHYSGSIGRAWLE
                     RGGVYVVANIRGGGEYGPSWHKAALKANRPKAYQDFAAVADDLIKRGVTSPQHLGAEG
                     GSNGGLLMGNMLTGYPQLYGAIACEVPLLDMKRYTHLSAGASWMAEYGDPDDPGQWAF
                     IKAFSPYQNVKPDMHYPDVLFYTTTSDDRVGPAQARKMAAKMEAMGYKNVWFYENTEG
                     GHGAGADNQQSATMHALAYDFLWDKLQ"
     gene            6295..>6440
                     /locus_tag="VFK00_08395"
     CDS             6295..>6440
                     /locus_tag="VFK00_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7593006.1"
                     /translation="MGLDLVPAGRDVPDDINVVIEIPKDAEPVKYEVDKASGAIFVDR
                     VLTTP"
BASE COUNT         1143 a         2126 c         2096 g         1075 t
ORIGIN      
        1 catgctgatt gccgcgctcg cgctcgcctt cgccattgcg gtcgcatggc tgttcaaccg
       61 gctggtgcgc ctgcgcaacc aggtgcgggt cgcgtggagc gacgtggacg tgcaactgca
      121 gcgccgccac gacctggtgc cgccgctggt ccagacggtg aaggcgtatg ccgcgcatga
      181 gagcggactg ctcgagcgcg tggccgtcga gcgccacgcg gcgctgagcg caacctccac
      241 gcccgatcgc gaaacctcgg aagccgcgct cggccggagc ctgacgcgcc tgatcgccgt
      301 ggccgaggcc tatcccgacc tcaaggccaa cggcaatttc atgcaattgt cgcggcaact
      361 ggtcgacacc gaaaacctga tccagcacgc ccgccgcttc tacaacggct cggttcgcca
      421 atacaacacc gcgatcgagc ggtttccggc cgtgctggcc gcgcgcccgc tcgggttcaa
      481 gccggccgca ttcttcgcgg ccgacgatga tgcgcgcggg aacgtgcagg tgggcctcgg
      541 atgaggaagc gcatgcgcca ccacgtattg ctgatggcgt tctgcctgtt cggccttggc
      601 gtcggcgtcg tcgatgccgg cgctgccgaa cgcatcctcg actaccactc ggacatcacc
      661 ctcgccgtcg acggcggcat gcaggtaacc gagacgatcc gggtgcaggc ggaaggcgcc
      721 cggatccgcc atggaatcta tcgcgacttt ccgaccgact accgcgaccg ccagcaccac
      781 cgttaccacg tcgggttcgc ggtgacccgc gccacccgcg acggtgcgcc cgaatcgtgg
      841 cgcacggagc ggcagtccaa cggcgtgcgt gtctatctcg gtgatgaagc cacgctggtc
      901 acgcccggcg tgcacacctg gacgatccgc tatcggacca atcgccagat cggtttcttc
      961 cccgaccacg acgagctgtt ctggaacgtc aatggcaccg gctggatctt cccgatcgac
     1021 cgcgcgagcg ccagcgtgca cctcccgctc gcggttccgg ctgcgaaact cgaggcctac
     1081 ggctacaccg gcagccagga ttcgcgcgag gaggcgctgg cggcgagcgt gcatgacggc
     1141 ggcgcggact acatcacgac gcgcccgctc gggtccaatg agggcctcag catcgtgctc
     1201 gagttcccga agggcgtgat catcgcgcct tcgaccgcgc agcggtggcg ctggtggctc
     1261 gccgacaact ccaacatcct gctggccgtg atcgcgctgg tcggcttgtg gatctactac
     1321 gtgcaggttt ggcgccgcaa gggccgggac cccgcagcgg ggccgttggt cgccgaatac
     1381 gagccgccgg atggcgactc cgcggccgcg ctccgttacg tcgaacggat gggctacgac
     1441 gatacctgtt tcaccgccgg catcctcggc gttgccgcca agggcgggct cgacatccgc
     1501 gaggacgacg ccaagaccta cgtcgccacc cggcgcgatg gcggcaaggc agtcttttcc
     1561 gccgacgaga aggcgctgcg ggacgccctg ttcgccggcg gcgccgacat cgaattcaag
     1621 cagggcgaac acagccgcgt acaggccgcg cgcagcgccc accagaaggc actgaaggcg
     1681 gcgttctaca gcaagtattt cctcaccaac agcttgtggc tgctgccggg agtcgcgatc
     1741 acggtcgtga gcgcatggct ggcgagcctc ggcggcgccg aaggcaccgg cttcctgctc
     1801 gcgtggatcg ggttctggac cgtgggtgtg gtcctgctgt tcaacagggc tgcgcacggc
     1861 ggccgcgccg cggtcggcgg ctggatcatg ttcgtcttct ttgcggcggc cgagctcatc
     1921 gcgatcgcgg cgctgggaac gctggccggc tacgcgctga ttccgctgtg catcgccctg
     1981 gtcgtgacca acctgatgtt ctaccagtgg ctgaaggcac cgacgcaact gggtgcgaaa
     2041 ttgctcgacc gcatccgggg tttccgctgg tacctcggcg ttgccgagaa acaggaactc
     2101 gacgcccgct acaagccgga atcacgaccg gacctgttcg ccgcctacct tccgtatgcc
     2161 ctcgcgctgg gcgtcggcaa cgcctgggcc gaacgctttt ccagtgcgct gtcgcccgtg
     2221 cagatggagc aggcgcgacc ctcgtggtat tcgggcacgg gcgccagcgg cttcaacggc
     2281 gcaagcttca cgaccttcgc cagcagcatg acctcggggt tcggcggcgc aatcgcctcg
     2341 gcgtcgatgg caccgggctc cagttccggc tcgggcggga gttcgggcgg cggcggcgga
     2401 ggcggcggcg gaggcggctg gtgagagatc ggtgcggagc ctggtctgtg caagcgccag
     2461 gctttccgtc acctgcaccg tcccttcgat ccgttcaccc tcgtcggaat tcaccagcgc
     2521 gcggcgacga tgacggcgat ccggtcggca ctggcaccgg gcatcatcgt ttcgggcatg
     2581 gctttgccga attcttccgc aaggagcaaa ggacccgggg cggtgtcttc gtcttgctcg
     2641 ccgtaccttt tgcctgggtc cggccacagc cgacgccaag cgcgcagagg aaagagtcaa
     2701 ccgcgtccgt cagttggttc gacaggccga gggtggtgtc gatggatcca tgatccaggc
     2761 cggtttgata ctccaccgcc ttgccatagg ccgcatcgac gaactcctcc gtctgccggc
     2821 acgatcccgg gtgtgcgatc gagcacacca tcagcatcgg cgggggcgcg gcggtaatgc
     2881 gcaacgtcgg cgaggcttcc gcccacagat tggggtccgc gccgaaaatg gggtcgtaga
     2941 gcgaatgcgg gtgcggggct ttcatgatct gcaccacgtt gagcgccgca ctgtcgagtt
     3001 cgatgttccc gagccacggt ttcgcgccct tgtggatgtc ggccgcggtt gtcatcaggc
     3061 ttgtcaggtg cgcgccggcg gaatgcccca tgacgaccac gcgattcggg tcgcctcccc
     3121 attgtgtcgc gtgcgcctgc acgtagatca atgctcgcgc cacgtccttc gcctggtcca
     3181 gcacgccgac ttgtggggaa agccggtagt tgacggaaac gaagatgatc cccttgggca
     3241 ggaagtggtt gatcttgttc tgcacgacac cggggaacgc cttgtctccg cgccaccagc
     3301 cgccgccatg caccatgaag acgatcggcg ccccgtgtac cccctttggg gcgtacacgt
     3361 ccagcttctg caacgggtcg ctcccgtagg ccacgttgga cgtcaggacg gcattggtcg
     3421 gaaggatgac cacggccaag aggggactcg agaacaacaa tgcgccgatg cacaacaacg
     3481 ctgcgaaggt gcgcgtcttg cgggcgttgc gggggaaagt aacctgggtc atttcactct
     3541 tcactggaag gatggttcgg tcgggacaac aggaatgctg tcgcgaaccg agtatccccg
     3601 cgggggaagc tggtgaaagt gacggttgcc tcaatatcgt tccgatcgcg caattcgctc
     3661 ggcgcatcga actccgtaca tctgccgtga tgtggttgaa cttgcccgca gctcacggca
     3721 gcgccggaac ggttctggta ggcactgtcc gcgcagatgg agaaggcaca actccggtgg
     3781 cattcctcga cgggcagcgg cggtttcacc gcgaggtttg ccagcagcat gtcatcgggc
     3841 gccatcgcat ccgcgtcgta gcgccggggc ccggttccgg gtcgggcaac gcatcggacg
     3901 gcggccgtgg cggctggtga acggttggac gcccagcgag gtgccatgcc tttcgtcacg
     3961 tgcgccgtcg cgccgatccg ttcaacatgg ccggaattcc acacgaggca gcgacgatga
     4021 aggcgatctg gccggcactg gcattgggcg tcatcgtttc gggcacggca ttggccaatc
     4081 cttccccaaa gaaccacgaa ccggcgtcga cggcatcatc cggcgacccg tatctctggc
     4141 tcgagcccag ccacagccaa cgtgcgatgg agtgggtgca cgcacagaac gcgatcaccc
     4201 agaaagcttt catggaatcg ccggagttcg acaagacccg cggcaagatc ctcgaagtgc
     4261 tcgactcgga ggcgcgcatt ccctacgtct accggatggg cgactacctg tacaacttct
     4321 ggcaggacaa ggccaatccg cgcggcatct ggcggcgcac cacgctggcc gaatacgaga
     4381 aggccgaccc gaagtgggaa acggtgctcg acatcgacgc gttgaacaag gccgaagaca
     4441 agaagtgggt gttcaagggc gcgcgctgca tgaagcccga ctacacccat tgcctggtgt
     4501 cgctgtcgcc gggcggcggc gatgcggtgg aggtgcgcga gttcgacctc ccgtcgaaaa
     4561 ccttcgtgaa tggcggcttc ttccttccgg tcgccaaatc ggacgtcgac tggatcgacg
     4621 ccgaccacat ctacgtcggc accgacttcg ggcccggctc gatgaccaag tcgagctatc
     4681 cgcggatcgt caaggaatgg acgcgcggca cgccgctttc ggcggcgacc gtggtgtacg
     4741 aaggcaagcc gaccgatctc gccatcggcg cctaccacga ccgcaccaaa ggctacgagc
     4801 gcgacttcgt ctcggtgcag aaggacttct tccacggcga catgtacctg cgcgacggcg
     4861 gcaagctcgc caaggtcgac gtgccggaag acgccaatgc cgacgtgcat cgccaatggc
     4921 tgctggtgca gacgaagtcg ccctggacga ccggcggcaa gacctggccg gccggcgcgc
     4981 tgatcgcgat gaagttcgag gatttcatgg ccggcaagcg cgatttcacc gcgctgttca
     5041 cgcccgatgc gcacaccgcg ctcgcgggtt actcgtggac gcagaaccac atcatcctgg
     5101 acgtgctgga cgacgtgaaa agtcgtctcg acgtgctgac gccaccggca tccggcaacg
     5161 gcgcatggca gcaccaggcg ctgccgggcg cgccgaagtt cagcaccatc gcggtggtcg
     5221 acaccgatcc cgaccactcc gacgaatact ggctcacagt caccgggttc ctcgatcctc
     5281 catcgctgga acgtggggta cttggccgcg aacccgcgaa gaagatcaag caggaaccgg
     5341 cgttcttcga cgccgcgaaa ttcgaggtca gccagcactt cgtgacctcg aaggacggca
     5401 cccgcgtacc gtatttccag atcgctccga aggacatgaa gctcgacgga agcaaccgca
     5461 cgctggaata cggccacggc ggcttcgagg tctccctgct gccgcactac tccggctcga
     5521 tcggccgggc gtggctcgaa cgcggcggcg tctacgtggt cgccaacatc cgtggcggcg
     5581 gcgaatacgg cccgtcgtgg cacaaggccg cgctcaaggc caaccgtccg aaggcgtacc
     5641 aggatttcgc cgcggtcgcg gacgacctga tcaagcgcgg ggtcacctcg ccgcagcacc
     5701 tcggggccga aggcggcagc aacggcggcc tgctgatggg caacatgctg accgggtatc
     5761 cgcaactcta cggcgcgatc gcctgcgaag tgccgctgct cgacatgaag cgctacacgc
     5821 atctctcggc cggcgcatcg tggatggccg agtacggcga tcccgacgat cccgggcagt
     5881 gggcattcat caaggccttc tcgccgtacc agaacgtcaa gccggacatg cactatccgg
     5941 acgtgctgtt ctacaccacg accagcgacg accgcgtcgg ccctgcgcag gcacgcaaga
     6001 tggccgcgaa gatggaggcc atgggctaca agaacgtctg gttctacgag aacaccgaag
     6061 gcggtcacgg cgccggcgcc gacaaccagc agtcggcgac catgcatgcg ctcgcgtacg
     6121 acttcctgtg ggacaaattg caataaaagc cggggtcaga gtgcaatttc gtcggaagct
     6181 gtcgcgttgc ggaggaaact tgcccgaaat ggcactctga ccccggtttt tccgcatcac
     6241 gcagcgtctg cccgccgcgc gtaacatggg cggtttccga aaggacaccc ccccatgggc
     6301 ctggatctcg tccccgccgg ccgcgacgtg ccggacgaca tcaacgtcgt catcgaaatc
     6361 cccaaggacg ccgaacccgt caagtacgag gtggacaagg cctccggcgc gatcttcgtg
     6421 gatcgcgtgc tgaccacgcc
//
LOCUS       DASSNH010000232         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_529317, whole genome shotgun sequence.
ACCESSION   DASSNH010000232 DASSNH010000000
VERSION     DASSNH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529317"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1152)
                     /locus_tag="VFK00_08400"
     CDS             complement(277..1152)
                     /locus_tag="VFK00_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593007.1"
                     /translation="MKAIVACVLSFVACGWAWAGVSFAGPAAGVQATTRPAAPFKIIA
                     TDSGFEAPATLSAGLRHIVFENRGSTIHEAMLVKLPDGMTPAAYVDRIRHGQLFPEGA
                     LDYSGAGLTSPGEAVEIWTRVDPGHYVLICWNGDHATTVPLRAFTVLADGSPDDTPPK
                     EDAVLKLGDFRFELHGRLRSGSRVIRVETPGPSMHELDIFRLRGDHTAADVNRWRKRE
                     DSGSPSETTGPADALGGLLDSHDIKRVSWLRQNFRPGRYVLHCEMPMDPTAVAGRVDV
                     THADAGMVQEFEVAP"
     gene            1434..2156
                     /locus_tag="VFK00_08405"
     CDS             1434..2156
                     /locus_tag="VFK00_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7593008.1"
                     /translation="MPRSPRVDLPGIAQHVIQRGNDRRPCFFVGIDYTRYLQDLREIA
                     RREGCAVHAYVLMTNHVHLLMTPGAAGRIGRTMQALGRRYVRYVNDRDGRSGTLWEGR
                     YKACPVGDDRYLLQCHRYIELNPLRAKMVADPADYAWSSHRCHAVGIPDPLITPHPAM
                     ARLGRTGEERRRNYRELVMQAVDPEETDTIRRHLQHQRIYGPDHFRLALERRLGRKLG
                     PRKIGRPKKAVIDTDGLLESRL"
     gene            complement(2169..2894)
                     /locus_tag="VFK00_08410"
     CDS             complement(2169..2894)
                     /locus_tag="VFK00_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593009.1"
                     /translation="MAGQQPDADRVDAAQQHLQARIDRYRPRTRAFRGVAWAGAACTA
                     VLAMALTLGPIFTGRGDVQAFAAVQHRLQDFKTLQMTIHQRANGIEQPTIRTWTDRAG
                     DARTDIGDGTSVIVNVQQRTVLTLLHAQRKAMRMPLHADAGNPAEKALGWIDTIRQFK
                     GNARPLAGTRLIDGVVTHGWALDTSGLHIELWADDDSVPRAVDISGGVNLHQRLKLIL
                     DQPIDPARFSTKLPSGYTLAQPD"
     gene            complement(2945..>3172)
                     /locus_tag="VFK00_08415"
     CDS             complement(2945..>3172)
                     /locus_tag="VFK00_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7593010.1"
                     /translation="AERSQQAARLRRALTRLPVREAECFALRCLHGMDVADIARSTGM
                     TANHVSVSLHRATRKLESALGTTTPTTEENA"
BASE COUNT          537 a         1047 c         1056 g          532 t
ORIGIN      
        1 gggccggtct tgaggatcac cagcacgtag tgccgcatgc cgtgctcgtc cgcgcccgtc
       61 ttcttcgcga gggacgcgtc ataagccggc ttggcgggcg gttgcgaaaa cgctggcccc
      121 gccgcggttg ccatcgcaat accgaaacac gccacccatc cgcgaatgtt catggcagtc
      181 cctccccggg atccgtggcg ggagtatgaa tccaaaaccg gggtcagagt cgacttttac
      241 cgacagccaa ccgccggcag cgacgcacac aaaccgctac ggggcgacct cgaattcctg
      301 caccattccc gcgtccgcgt gggtcacgtc gaccctgccc gccaccgcgg tcgggtccat
      361 cggcatttcg cagtgcagca cgtagcggcc gggtcggaag ttttggcgca gccagctcac
      421 ccgcttgatg tcgtggctgt cgagcagccc gcccagggca tcggccgggc ccgtggtttc
      481 ggaaggagac cccgagtctt cgcgcttgcg ccaacggttg acgtcggcag cggtgtggtc
      541 gccgcgcagg cggaaaatat cgagttcgtg catcgagggg ccgggcgtct ccacgcgtat
      601 cacgcgcgag ccgctccgca agcgcccgtg gagttcgaag cggaaatccc ccagcttcag
      661 gaccgcatct tccttcggcg gggtgtcgtc cggtgaacca tcggcgagca ccgtgaaggc
      721 tcgcaacggc accgtggtcg cgtggtcgcc gttccagcag atcaggacgt agtgtcccgg
      781 gtcgaccctg gtccagattt ccacggcttc gccgggcgac gtcagccccg ctccggaata
      841 gtcgagcgcg ccctcgggaa agagttgtcc gtgccggatg cggtccacgt aagcggctgg
      901 agtcatgcca tcgggcagtt tcaccagcat ggcttcgtgg atcgtcgagc cacggttctc
      961 gaagacgatg tgccgaagcc cggccgacaa cgtggccggg gcctcgaagc cggaatccgt
     1021 cgcgatgatc ttgaacggcg cggctgggcg cgtggttgcc tggacgccgg cggcaggccc
     1081 tgcgaaggac acccccgccc aagcccaacc gcaggccacg aacgagagga cgcaagccac
     1141 gatcgctttc atgacgcctc ccgattgtgc ttgatggccc gccacttgaa tgcgaaacac
     1201 aaaccggatt ctggattccg gcctgcgccg gaatgacgcg caaaagctca agcgccgcgg
     1261 caacgcgtga tatccaacgg cgccccctac gcgatgtccg cgatgctact ccatcacccg
     1321 gaaaaccggg gtcagagtcg acttttcccg acaggccttc ccggccaccg ccgacagcat
     1381 cgcatgcaag ccgacgcgag aatgccgcag tccgtcttgc aaggattgcg gccatgcccc
     1441 gctctccccg ggtcgacctt cccggcattg cacaacacgt gatccagcgc ggcaacgatc
     1501 gccgcccgtg cttttttgtc gggatcgatt acacccgtta cctgcaggat ctgcgcgaga
     1561 tcgcgcggcg cgaaggctgc gccgtgcacg cgtacgtcct gatgaccaat cacgtgcatc
     1621 tactgatgac acccggcgcc gcgggtcgca tcgggcgcac catgcaggcg ctggggcgtc
     1681 gctacgtccg ctacgtgaat gatcgggatg gccgaagcgg aacgctgtgg gaaggacgct
     1741 acaaggcctg tccggtgggc gacgatcgct acctgttgca gtgccaccgc tacatcgaac
     1801 tgaacccgct tcgcgcgaag atggtggcgg acccggccga ctacgcctgg tccagccatc
     1861 gctgccacgc cgtcggcata cccgatccct tgatcacgcc gcatcctgca atggcgcggc
     1921 tgggccgcac cggcgaagag cggcggcgga attaccggga attggtcatg caagccgtcg
     1981 atcccgaaga aaccgacacc atccgccggc acctccaaca tcaacgcatc tacggccccg
     2041 accacttccg ccttgcgctt gaacgacggc tgggaagaaa gctcggcccc aggaagatcg
     2101 gccgtccgaa aaaggccgtc atcgacactg acggcctgct ggaaagtcga ctctgacccc
     2161 ggttttcctc agtcgggctg cgcaagggtg tagcccgagg gcagcttggt gctgaagcgt
     2221 gccggatcga tgggctggtc caggatcagc ttcaagcgct ggtgcagatt cacgccgccg
     2281 ctgatatcga ccgcacgcgg cacgctgtcg tcatcggccc agagctcgat gtgcaggccg
     2341 ctggtgtcca gggcccagcc gtgggtgacg acgccatcga tcagccgggt gccggccagg
     2401 ggcctggcgt tgcccttgaa ctgacggatc gtgtcgatcc agcccagcgc cttctcggcg
     2461 ggattgcctg catcggcatg caatggcatg cgcatggcct tgcgctgggc atgcagcagg
     2521 gtgagcacgg tgcgctgctg gacgttgacg atgacgctgg tgccgtcccc gatatccgtg
     2581 cgcgcgtcgc cggcacgatc ggtccaggtg cggatggtcg gctgttcgat gccgttggca
     2641 cgctgatgga tggtcatctg cagtgtcttg aagtcctgca gccggtgctg cacggcagcg
     2701 aaggcctgga cgtcgcctcg gccggtaaaa atcgggccca gcgtcagggc catggcgagc
     2761 acggcggtgc atgccgcgcc ggcccaggca acgccgcgaa acgcgcgcgt gcgcgggcgg
     2821 taccggtcga tacgagcctg caggtgctgc tgtgcggcat cgacgcgatc ggcatccggc
     2881 tgctgcccgg ccatggcgcg gacatgggat tcgaggttcg gggacaaggg cgtgttggtg
     2941 ttcatcaggc gttctcctcg gtggtcggcg ttgtcgtccc cagcgcggac tcgagcttgc
     3001 gcgtcgcgcg gtgcagactg acgctgacgt gattggcggt catgccggtg ctgcgtgcga
     3061 tgtcggcgac atccatgccg tgcaggcagc gcagggcgaa gcattccgcc tcgcggacag
     3121 gcagccgggt cagcgcgcgg cggaggcgag cggcctgctg cgagcgttcg gc
//
LOCUS       DASSNH010000233         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1712426, whole genome shotgun sequence.
ACCESSION   DASSNH010000233 DASSNH010000000
VERSION     DASSNH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712426"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFK00_08420"
     CDS             complement(<1..334)
                     /locus_tag="VFK00_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HET7593011.1"
                     /translation="MRQAIPANLDESRLAMLVERFYDKVRADPLLAPVFNPVVDDWDA
                     HKVLMTSFWATVALRTGHYRGNPLARHQPLPVGVEHFRRWLALWRETAGELLDAEPAA
                     LMTGYAERI"
     gene            453..800
                     /gene="fdxA"
                     /locus_tag="VFK00_08425"
     CDS             453..800
                     /gene="fdxA"
                     /locus_tag="VFK00_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HET7593012.1"
                     /translation="MTHVVTDNCINCKYTDCVEVCPVDCFHEGPNFLVIDPGECIDCT
                     LCVAECPVFAIFPEMDVPAGQEHFLGINAELAKNWPVIASKKPEMEGADLWDGIPDKL
                     PLLDRGDAKADAA"
     gene            886..1386
                     /gene="tpx"
                     /locus_tag="VFK00_08430"
     CDS             886..1386
                     /gene="tpx"
                     /locus_tag="VFK00_08430"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7593013.1"
                     /translation="MSSVNFKGNPVHVDGQFPKVGDKAPAFRLVAGDLSEKTLRDFAG
                     KRKVLNIFPSIDTGVCAASVRHFNKDAAGLRNAVVLCISADLPFAQARFCGAEGIENV
                     VMLSMMRGREFLKDYGVAMTDGPLAGLAARAVVVLDENDKVIHAQLVDEIGHEPDYDS
                     ALKALG"
     gene            complement(1626..2633)
                     /gene="gap"
                     /locus_tag="VFK00_08435"
     CDS             complement(1626..2633)
                     /gene="gap"
                     /locus_tag="VFK00_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7593014.1"
                     /translation="MAIKVAINGYGRIGRNILRALYESNRTGAIRIVAINDLGDAETN
                     AHLTQYDTAHGKFPGTVGVEGGDLVVNGDHIKVCAERDPSKLPWKDLGVDVVIESTGL
                     FTQRDKAAAHLAAGAKKVVISAPGKDVDGTFVFGVNHDQIKPSDTIISNASCTTNCLA
                     PLAKVLHEKIGIVHGLMTTIHAYTNDQVLTDVFHKDLRRARSATHSQIPTKTGAAVAV
                     GLVLPELNGRLDGFSMRIPTINVSCVDLTFVAQRETSREEIDAAINEAANGRMKGVMA
                     VNTKPLVSIDFNHNPASCTYDATQTRVMGGTLVKVLGWYDNEWGFSNRMLDMTEAFAN
                     A"
     gene            2751..>3154
                     /locus_tag="VFK00_08440"
     CDS             2751..>3154
                     /locus_tag="VFK00_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET7593015.1"
                     /translation="MRRTFHTRRRARAVFAALAMLACTPPASAWGPLGHRMVARLAEA
                     RLTPEAEAEVKALLAARGERYLDQIANWADELRDTDPALYRRTARLHYVNFGSRDCIY
                     VPPRDCRDGECVVAGIAKYSAILADRSNGIAER"
BASE COUNT          511 a         1002 c         1064 g          577 t
ORIGIN      
        1 cgatgcgttc ggcgtagccg gtcatcagcg cagcgggttc ggcatccagc agttccccgg
       61 cggtctcgcg ccacagcgcc agccagcgtc ggaagtgctc gacgccgacc ggcagcggct
      121 ggtgcctggc cagtggattg ccgcggtagt ggcccgtgcg cagtgcaacc gtcgcccaga
      181 acgacgtcat caggaccttg tgtgcgtccc agtcgtcgac caccgggttg aataccggag
      241 cgagcaaggg gtcggcgcga accttgtcgt agaagcgctc caccagcatc gcgagccgcg
      301 attcgtcgag gttggcaggg atggcttgcc gcattcccga agcgtcgcct ttcgcgcctc
      361 gcgacgccat gacctgcatc aatgacgcgt atcaatgcgc acccatgcgg attgcgacac
      421 agtgcgatcc cgccgacatg ccggcaaacg ccatgaccca cgtcgtcacc gacaactgca
      481 tcaactgcaa gtacaccgat tgcgtcgagg tgtgcccggt cgattgtttc cacgagggcc
      541 cgaacttcct cgtgatcgat cccggcgaat gcatcgactg caccctgtgc gtggccgaat
      601 gcccggtgtt cgccatcttc ccggaaatgg acgtgccggc gggccaggag catttcctcg
      661 gcatcaatgc ggaactcgcg aagaactggc cggtgatcgc cagcaagaag ccggaaatgg
      721 agggcgcgga tctgtgggac ggcattcccg acaagctgcc gttgctcgat cgtggcgacg
      781 ccaaggccga cgcggcctga ccccgacgcc gccgtgggct agactgaaag gatcgcggag
      841 cgcacggcaa cgcacctcag tgatccacac gcaaggaggt ccaccatgtc cagcgtcaac
      901 ttcaagggca accccgtcca cgtcgacggc caatttccga aggtcggcga caaggcgccc
      961 gcgttccggc tggttgccgg ggacctgtcg gaaaagacgc tccgggattt cgccggcaag
     1021 cgcaaggtcc tgaacatctt cccgagcatc gataccggtg tctgcgccgc ctcggtgcgg
     1081 cacttcaaca aggacgcggc gggattgcgg aatgccgtgg tgctgtgcat ctcggccgac
     1141 ctgccgttcg cacaggcgcg tttctgcggc gccgaaggga tcgagaacgt cgtgatgctc
     1201 tcgatgatgc gcggccgcga gttcctgaag gattacggcg tcgcgatgac cgatggtccg
     1261 ctggccggac tcgccgcgcg cgcggtggtg gtgctcgacg aaaacgacaa ggtcatccac
     1321 gcgcaattgg tcgacgagat cggccacgag cccgattacg attcggcgct gaaggcgctc
     1381 ggctgagaac acaggccggc gctttctccc gaaggcgcct tccggctgct tcgaactttc
     1441 tcgaggagcc gcttgttttc ccccttccgc aggaagggcg gagcgaacgc gcgcgaagcg
     1501 cgacgagggg gtggctttcg gggcgggctg tcgtctccgg tgcatccccc gaagccgcct
     1561 cgcggcggct tcgaccccct tgcttcggaa gggggtgaat gggctgcgac cgtagcgccc
     1621 cgctcctaag cgttcgcgaa cgcctcggtc atgtcgagca tgcggttcga gaaaccccac
     1681 tcgttgtcgt accagccgag taccttgacc agggtgccgc ccatgacgcg ggtctgggtg
     1741 gcgtcatagg tgcaggacgc ggggttgtgg ttgaagtcga tcgacaccag cggcttggtg
     1801 ttgaccgcca tcacgccctt catcctgccg ttcgccgctt cgttgatcgc ggcgtcgatt
     1861 tcttccctgc tggtttcgcg ttgcgcgacg aacgtgaggt ccacgcacga tacgttgatc
     1921 gtcgggatgc gcatcgagaa accgtccaga cgtccattca gctccggcag gaccagtccg
     1981 acagcgacgg cggcgccggt cttggtcggg atctggctgt gggttgccga tcgcgcgcgg
     2041 cgcaggtcct tgtggaagac gtcggtcagc acctggtcgt tggtgtaggc atggatggtg
     2101 gtcatgaggc cgtgcacgat gccgatcttc tcgtgcagca ccttcgccag cggtgcgagg
     2161 cagttggtgg tgcacgaggc gttggagatg atcgtgtccg acggcttgat ctggtcgtgg
     2221 ttgacgccga agacgaaggt gccgtccacg tccttgccgg gcgcggagat gacgaccttc
     2281 ttcgcgccgg ccgcgaggtg cgccgcggcc ttgtcgcgct gggtgaacaa gccagtcgac
     2341 tcgatcacca cgtccacgcc caggtccttc cacggcagct tggacggatc gcgttcggca
     2401 cacaccttga tgtggtcgcc attgaccacg aggtcgccgc cctcgacacc gacggtgccc
     2461 gggaacttgc cgtgcgccgt gtcgtactgc gtcaggtgcg cgttggtttc ggcatcgccc
     2521 aggtcgttga tcgcgacgat gcggatcgcg ccggtgcggt tggattcgta cagcgcacgc
     2581 aggatgttgc ggccgattcg gccgtagccg ttgatcgcga ccttgatcgc catggtgtgt
     2641 cctttgccaa gcagtgtggg gaaacgccaa ttttagcttg tttgccgggg cgcttcatgc
     2701 gcgacggcgg cgtgcgacgt ggcaaactgg cgttttcctc cggagccgtg atgcgccgga
     2761 ctttccacac gaggcggcgt gctcgagccg tcttcgcggc cttggcgatg ctggcgtgca
     2821 cgccgccggc ctcggcctgg ggaccgctcg ggcaccggat ggtggcgcga ctcgcggagg
     2881 cgcgtctgac gccggaagcc gaggccgaag taaaggcgct gcttgcggca cgtggcgagc
     2941 ggtacctcga ccagatcgcc aactgggccg acgagctgcg cgacaccgat cccgcgctgt
     3001 accgacgcac ggccaggttg cactacgtca atttcgggtc ccgcgattgc atctatgtac
     3061 cgccgcgcga ctgccgcgac ggcgagtgcg tcgtggccgg gatcgccaag tactcggcga
     3121 tcctggccga ccgctcgaac ggtatcgccg agcg
//
LOCUS       DASSNH010000234        22847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1084117, whole genome shotgun sequence.
ACCESSION   DASSNH010000234 DASSNH010000000
VERSION     DASSNH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 22847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22847
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084117"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..337
                     /locus_tag="VFK00_08445"
     CDS             71..337
                     /locus_tag="VFK00_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593016.1"
                     /translation="MDKRLLDILCDPVTKTPVRPLRRDELDALNRAISAGQIDTVAGQ
                     KVSAPLAAGLITTDRRVVYRVEDDIPVMLADEGIGTLQLTDFPG"
     gene            511..2886
                     /locus_tag="VFK00_08450"
     CDS             511..2886
                     /locus_tag="VFK00_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HET7593017.1"
                     /translation="MTPIPTDPPADQQRNATTKVVEIGSRATDADHDHVGGLLAAVRD
                     MTVKEAANLVAGLLDNVDDTLFDLAEKAESNASQTEYFDGMRETRKRRPRIERLFSER
                     VTRGFTDFAAGRAARPGSEAQKAFSASGELSLVDDRELEESLAVSSMVAKADGRLSSA
                     LVALNQRLSVLSGGRTTTNANNPLGPNALTEAFRVALGELTDINIRVRLIILKMFERY
                     VLNALDALYHDVNMRLVQAGILPQLRRPAVVRGHGYTGGTTRAQTTAPDEAVEAADDG
                     EYDTADQLHSELMQRLTSLLAARRHGSAGMGGGRMSAGAGAGIASLSPAELLSALTLL
                     QGETRPLPSQPIATDTNSAAEVVQHLKDALMAQIQRLSGEAKARISGSDEDTIDLVGM
                     LFEYILQDHTIPAPMQVLLSRLQIPYLKVAILDRRMFAHASHPARKLLDQLADAAKGW
                     SEESDRDHRLFGKVKAVVETLLQDFDDDIGVFERQLADFTQFAEQNRKRAELAESRAT
                     EAARGRERLQDARRRAAQEILSRISGRNLPTLLRGVLTRPWANYLVLIVLRQGVESQE
                     FRSAVRFIDDFVASADAPQGEAAHAEYKNALPSIEKHLRTGLATVAFQEPDIERLLDE
                     LRKFWRQQLGEPAPVAPADAPAVDAAVLGMNQDAQPAITDAAAEAEEDFESDDMSVDI
                     DAGSLQAIRELKVGGWVEFIDEQGTRERAKLSWISPISGKYLFVNRRGLKVADRTAVQ
                     LANELQGQRLMILEEVPLFDRALDAIVDRLRNAHAEQPPQQLQQQPQQPEG"
     gene            2883..3743
                     /gene="nadC"
                     /locus_tag="VFK00_08455"
     CDS             2883..3743
                     /gene="nadC"
                     /locus_tag="VFK00_08455"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET7593018.1"
                     /translation="MSGPESRQPLPPQEAIRADVARALAEDIGSGDVTAALIDAGAIA
                     SARIVSREQAVLAGSAWADECFRQLDAGVHIDWLAVDGEPIAPDTELCHIRGNARALV
                     AGERTALNFLQTLSATATTTRAYVDAVAGTRAKILDTRKTLPGLRLAQKYAVRCGGGD
                     NHRIGLFDAILIKENHVAAAGGIAAAIHAARAAHPELLLECEVESLDELREAIDAGAD
                     RVLLDEFTDSMLREAVALAAGRMPLEVSGSVYLERVRVIAETGVDFISIGALTKHVRA
                     IDLSMRFDNA"
     gene            complement(3910..4122)
                     /locus_tag="VFK00_08460"
     CDS             complement(3910..4122)
                     /locus_tag="VFK00_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593019.1"
                     /translation="MDGIDANQTERVLSYLARLKHQGDPAQMDTLESLRPSWLGMDAW
                     DVVLEDLEAQHYRNRARVVRLQVVAG"
     gene            complement(4330..8226)
                     /gene="hrpA"
                     /locus_tag="VFK00_08465"
     CDS             complement(4330..8226)
                     /gene="hrpA"
                     /locus_tag="VFK00_08465"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET7593020.1"
                     /translation="MEKQSNDPTLRNLQRAMGSVLSRDAGKLRARLRRLERERPRSAG
                     TVGALAGEIAASAARREARAALVPPIAVDASLPIAQRADEITRLIRRHQVLVIAGETG
                     SGKTTQLPKLCLAAGRGVAGMIGCTQPRRLAARAMARRVASELGGGHNDQPGGIVGYE
                     VRFARQLGTDTLVKFMTDGILLAEMAHDRWLSEYDTIILDEAHERSLNIDFLLGYLKH
                     LLRRRRDLKLIVTSATIDTARFAEHFDGAPVLTVEGRGFPVELRWRAPEGDAGLTERV
                     VDALDDISREDPRGDVLVFLPGEREIRDAHLALSRRQYHHTEVLPLYARLSVKEQDRV
                     FKPGPARRIVLATNVAETSLTVPRIRWVVDSGDARVKRYSRRSQIERLHIEPVSQAAA
                     NQRAGRCGRVGPGICVRLYDEADFAARPPYGDPEILRSALSDVILRMLDLGLGDVEAF
                     PFIDPPDPRAVNDGWRRLAEIGAVDGERRLTPMGRQLARIPVDAQLARMIVEAGTLGV
                     AREVLPIVAFLGTQDPRERPADKQAQADAAHRAFADPRSDFIGVLNLWDAYRSAHEEL
                     SQSKLRDWCGKNFVSFMRMREWGALHRQLLLEAGTGDQGPGTGKAAGPRSPVPGPELY
                     EPVHLALLAGLPTNVARQDEQGLYRGARERKFRIFPGSALSKKPPPWVLVAQILDLGA
                     KVWGMQCARIEPTWIERQAAHLLRRTCSDPHWSRARGSVQAFEQVSLYGLVLVERRAV
                     TFGRQDPALAHDIFLREALARCDFDSRADFVGANRRVLDEAREIEAQQRREGLVKSDE
                     DLAGFFRGKVPDMISTTAALDAWFRRAAASERAAMRWSLDDVLEAGAAGAAGAFPEAL
                     EVSGQALHLEYRFVPGDDADGVTLRVPLALLNAVSAARCEWLVPGLLADKVTALLRGL
                     PKALRRNFVPAPDFARAFVEAEPPRDEPLAHALAAFLKRITGVAIDSAALDEASLPAH
                     LRMRFDVRDEQDRSLAAGRDLAALRAGWAARARKAFSRAAELPVTREEVRTWDFDEIP
                     VVSETRAGLPAYPALVDLGSAVALRVFETGQEAITAHRAGVERLLRLSLEGEFKRARK
                     QLPLKPRLAIAFAPHGRADTLREDVVEGGFVDLLEALDLDVRDRVAWQALHEALARGV
                     FGAAMERLKLAEPILVALSDLQPWMTPAIKAQAPASYADLRGQLDALLAPGFLRELPR
                     SRLVHLPRYLKAMRLRAEKLRGDPGRDHSRLQQVLPYWRRVLDARAAGDKSEALDALR
                     WLVEEWRVSVFAQELKTAGPVSGKRMAQALAALGGV"
     gene            8374..10494
                     /locus_tag="VFK00_08470"
     CDS             8374..10494
                     /locus_tag="VFK00_08470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HET7593021.1"
                     /translation="MPRTTLILAAAIACACAACSQQAPTGPVEAAAAAPAATAANPAS
                     ASTAPDIGIDLSYVDHSVKPGDDFFKYANGAWLETAKIPADRSSIGAFFDMSRRTEER
                     TADLIENAGKGHPPAGSNARKIADYYAAWMDTDAIEQHGLAPLKGELDAIDGIQSRAD
                     LARVLGSRLRQDVDPINATDFHTDHLFGLFVAQGLSDPSHNIAYLLQGGLTMPGRDYY
                     RSDDKTMAGYRDQYKRYIAAIFDAAGIKDAQAAAGRVFDLETKIAMAQESLVDSEDVH
                     AANNLWSLADFGKKAPGLDWNAYFKAAGLGGQKIIDAWQPGAIARLSKLTADAPLESW
                     KDLLTFHTLDRHADLLPKKFADLSFQFHSHALQGVPQQRERSKRAVDATSEALGFAVG
                     QLYVERYFPASAKQQVEQLVQNLIAAFNTRLETLEWMTPSTREKARAKLKTLRVQVGY
                     PDHWPSYDGLEITADDPLTNELNVSRFEYRQRLAVLGKPVNRDHWWMTPQLVNAINLP
                     LENELQFPAAILQPPFFDPKSDAAANYGAIGAVIGHEISHSFDNMGSQFDAEGRLHNW
                     WTPADAEHFKEATERLVKQYDQYEALPGLHIDGQQTLGENIADVSGLTIAHLAYHKSL
                     GGQPAPVIDGLTGDQRFFLAYGQTWREKMRDAALRQRVQTNVHAPGEFRAETVRNLDA
                     WYKGFDVQPGAKLYLKPDQRVQIW"
     gene            10612..11133
                     /locus_tag="VFK00_08475"
     CDS             10612..11133
                     /locus_tag="VFK00_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HET7593022.1"
                     /translation="MFVRVSLCALLIGFAGVAGAQTQPAAAPQKAPPQQQLTPQQREA
                     LQKQNQVLIQYADRIVAMLDNGQAGQVWDQASDVAKKSVSRDQFINTVQSDHARLGAM
                     KSRKLAVVTHTLSKGDGKLPAGQYVNINYATEFGSQAKPTRELLSFHLDSDRKWRLSG
                     YTLHAAENTVSRK"
     gene            11414..12004
                     /locus_tag="VFK00_08480"
     CDS             11414..12004
                     /locus_tag="VFK00_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7593023.1"
                     /translation="MSRMIHRSLSLAIGTTAVLAFAGCTNYVKKTDFNAAIAQLQSKD
                     QSLQQQIDSLSQEMKAGFAKYDTEITAMQGRISVNDIVHFGFDQSTIPEQAKPKLDEF
                     AKIMQAHHSDALVTVEGFADPAGSSAYNKRLGQARAKAVRDYLVNNDGMAADQVRVVS
                     YGESRNRQVKPGATHAAGVPNRRATLVVDFAGSTES"
     gene            complement(12093..13493)
                     /gene="murD"
                     /locus_tag="VFK00_08485"
     CDS             complement(12093..13493)
                     /gene="murD"
                     /locus_tag="VFK00_08485"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7593024.1"
                     /translation="MRFAELERADLAVWGWGREGRSVLAALRRRFPDKPLTLYCAPDE
                     AAALRKESTSFPSAVEGKGIDSESLDLRTRPPDADALSSHGIVIKSPGISAYKPEILA
                     ARECGTRFTSGTALWFDENPDARVVAVTGTKGKSTTTALIAHLARGLGVRTALAGNIG
                     FPLLDLDGQRADLWALELSSFQTGEAGPLELGVVVSLAEEHLDWHGSRERYVADKLKL
                     ADASRTLLVDAGSPLLMERTATHPHRAVFGEACGWHVAGGAIHRGSEAIIELDDLPLR
                     GAHNARNACAALAAIELLGMDARGAAGSLRTFRPLPHRLTRLGTRDGIEWIDDSISTT
                     PDAALAALESLRGRDIVLIIGGFDRGLEWGAFADELAARPPHAVIVQGAAGPRILDAL
                     RRARVGCTIDEAADVALAVTRARALAPAGGVVLLSPGSPSFDQFKDYAERGRTFARLA
                     GFDEEAIGAMAGLGIA"
     gene            complement(13474..14892)
                     /gene="murL"
                     /locus_tag="VFK00_08490"
     CDS             complement(13474..14892)
                     /gene="murL"
                     /locus_tag="VFK00_08490"
                     /EC_number="5.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-
                     glutamate epimerase"
                     /protein_id="HET7593025.1"
                     /translation="MNMLVDPRQAERFRFVRCGYDDGVAELVYAFDEGPDLVERIRFP
                     DAPPLQEERRQAFDAALRLLHLVAGVSYYKAGIPPAIQVENGALDAALADLLDTLYRH
                     GLAEFAYRNGIDLDGRIAFPRGAPLANDLPVSSPRRRPGSSAFAVPGAGEAATLDLPH
                     RTLVPIGGGKDSLVVVESLKSIGVGATAAWVGASPLIATVAARTGLPMLNLGRELSPV
                     LFEYNRMGAWNGHVPVTAINSAILACAAVLYGFDSIAFANERSASAATLEYAGRQVNH
                     QWSKGRDFENEFAANLRSRVAADLDYFSLLRPFSELAVTRAFAKSAVDYFDVFSSCNR
                     NFRILGPKPVDRWCGKCPKCHFVFLALAPFLPKPRLLAIFGRNLLDDETQADGFDALI
                     EYHDHKPFECVGEAAEARAAFATLAARPEWREDALVARFREDILPQLDASTLALAPWL
                     QPAGEHRIPTRFSGALDAIRGA"
     gene            complement(14889..17846)
                     /locus_tag="VFK00_08495"
     CDS             complement(14889..17846)
                     /locus_tag="VFK00_08495"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7593026.1"
                     /translation="MTASNPAPLPPDAPWVVLKFGGTSVATVDRWHTILRLAAARRAE
                     GLRVLVVVSALAGVTDELKALCACRADEREGALVKLVARHRELAAQMELANTAAMERW
                     LASLQALAFPLPSGEGGASAPGEGSGIRPSLKDPVPSPQPLSRGERGYAWHAAMQAHG
                     ELLSSSLGAAFMTANGLATEWLDARDALQARELPFLNERARMLSAQVQAEPDPAFAAA
                     LAARGEVFITQGFIARDASGATVLLGRGGSDTSAAYMGALLRAARVEIWTDVAGMFTA
                     DPRQVPVARLLRHLDYEEAQEIATTGAKVLHPRCLSPCRRARVPLWIKDTRQPDLEGT
                     VIGPQPAEAVPSIKAISSRRGITLVSMETSGMWQQVGFLADVFALFKQHGLSVDLVGS
                     AETNVTVSLDPAENLLDSDVVASLAADLARVCRVKVISPCAAVTLVGRGMRSMLHRLS
                     GVLAEFGRERVHLISQSSNNLNLTFVVDEGMLDGLTLRLHELLIRAGALRTDDAGLFG
                     PAWQALYGGGGARTRAPWWQGARETLLELAGSPSSPAKRGTGGEGSTELNHLADPEPS
                     PALTRAIHGARPSGRQMPFTADPVGRSRAPSPEGRAEKVTTPRYVYHLPTIRERARTL
                     RGLQSIDRVYYAVKANAHPAILAVLAKEGLGFECVSPGELVAVEAIGEARESSRLFTP
                     NFAPRSDYAEALQNQGGAVAVRSGSSPSPKSRVPSPELFLTLDALHPLQHWPGIFRGR
                     EIMLRIDLGRGAGHHDKVRTGGSTSKFGLPLEELDAFLALARGLGVRVAGVHAHLGSG
                     ILDASHWALVCSQLAGIADRIGTVRCLNLGGGLGVPSHPGEAELDLDVLDSALAEVRR
                     TCPQYELWLEPGRWLVADAGVLLARVTQTKRKGALNYLGCDAGMHALIRPALYDAWHE
                     IVNLTRIDAPADTLYQVVGPICESGDVLGADRHLPESREGDVLLIAQAGAYGESMASH
                     YNLRSLGESVVIE"
     gene            17996..18520
                     /locus_tag="VFK00_08500"
     CDS             17996..18520
                     /locus_tag="VFK00_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7593027.1"
                     /translation="MQQTPQNPPASIVPEPIRRGIDLRINGKQFSHTGDSDLPLLWYL
                     RDVLRLTGCKYGCDDASCGACSVLVDGKLEHACTLAMQDLDGREVTTVEGLAGPDGAL
                     HPVQQAWIDEDAILCGYCQPGQIMAAMDLLSRKPKPSDKDIDGIGNLCRCGSYPRIRR
                     AIKRASKAMQGKAK"
     gene            18517..20760
                     /locus_tag="VFK00_08505"
     CDS             18517..20760
                     /locus_tag="VFK00_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7593028.1"
                     /translation="MKRPHDAGRRRFLKVVGAATGGLVVGLPAFGWVPDELLGQNLVR
                     LGPYVRIEPDGTTVIGSRDPEVGQGIRTAEARILAEELDADWTRVVVEPLDLGVTAVG
                     GEPHWTLGHQVASGSTSIPAAWNDLRIVGAAARELLARAAAERWKTTIGNVRTVRGVA
                     IAADGRRLGYGDLAHAASQLQAPAAAPAPKLPSQYVLVGQDAGDVDARDIVTGVQRYA
                     IDEWFGDVLVAVITRCPWPGGGLARLDDRAALKVKGVEKVMTIPAPDPARAPGTQQLA
                     AGIAVLATDTWSALQGVAKLDVEWQPGQAVPEGDGALGKSAEATMAGDPTKAVRTDGD
                     FAEALKSARSTFKAEYRIATVAHADLEPPGALVRVDHQQALIIAPVQNPRATYETVRR
                     LTGLAPDKIDVRLPRAGGGFGRFLESDYVAEAVMLAKAAGKAIKLMWTRGDAFTHDLY
                     RPFSVHALEACADRHKALTGWSHHIASTSRLAGREPRSRWWLSEMHPDDLPAGLVDNL
                     QYAWYALDSTLPRGNYRASSHAVNAFATECFLDEIAQTLKQDALEFRLALLGEPRKLP
                     YRGHGGPVLDTGRLAWVLKLAADAIGWSKPHTQGHGFGLACHFTFGGYVAHAMEVSME
                     GNRLLIHNAVCAADIGRVVNPLGARAALVGATLDGLSTALRERITVGKDGRIAQQGFK
                     DYPLATMAQMPHDVQVILVPSQESPAGAFAMGFPSAAPALANAIFAGTTVRVHQMPIW
                     PELMRLL"
     gene            complement(20939..21856)
                     /gene="truB"
                     /locus_tag="VFK00_08510"
     CDS             complement(20939..21856)
                     /gene="truB"
                     /locus_tag="VFK00_08510"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7593029.1"
                     /translation="MTSAESSSRSPVTGILLLDKPTGISSNRALQQAKQLFGASKAGH
                     TGSLDPLATGLLPVCFGEATKVAGYLLGARKAYAAECRLGTTTDTCDSDGTVLLEREV
                     PTLEDNAISRELAKLTGRITQVPPIYSALKQGGVPLYKRARRGEAVEAAPREVDVFRF
                     DLTGRSANTLRLYVECGSGTYVRSLVRDLGEALGCGAHLTSLRRLWVEPFRDPRMFTL
                     AELESLRDRDPAALHECLFPIDAGLADWPTLQLDERGADDVAHGRALRVTAAPGRRRA
                     YAPDGGLLAIGEIDAAGTFKVLRGFVDGR"
     gene            complement(21970..22356)
                     /gene="rbfA"
                     /locus_tag="VFK00_08515"
     CDS             complement(21970..22356)
                     /gene="rbfA"
                     /locus_tag="VFK00_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029216910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7593030.1"
                     /translation="MPTRSFHRTDRVNAELRREVGVLVHAAVRDHALPQASVVDVDVS
                     RELDVATVWVTALQPERSAEVVKALKALAREFRHQLAHTMRMRRVPELRFKYDDSVDK
                     GERIERLLREEGDRGPGTGDQSASSS"
     gene            complement(22521..>22847)
                     /locus_tag="VFK00_08520"
     CDS             complement(22521..>22847)
                     /locus_tag="VFK00_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET7593031.1"
                     /translation="ASGLMGTEVREEILGVAEVRDVFRSSKFGAVAGCMVIEGTVRRN
                     KPIRVLRDDVVIFEGELESLRHFKENVEEVRNGSECGIAVKQYNDVKPGDRIECFERI
                     EVARTL"
BASE COUNT         3981 a         7978 c         7499 g         3389 t
ORIGIN      
        1 accccgacgc ggggcgcgac cgtcgttttc cgcatggtgt gtccgtcgca aaacggctat
       61 tgtacgcgcc atggacaagc gattgctgga catcctctgc gacccggtca ccaagacccc
      121 ggtgcgtccg cttcgccgcg acgaactcga tgcgctcaac cgcgcgattt cggcgggcca
      181 gatcgacacc gtggccggac agaaggtctc cgcgccactc gcggcgggac tcatcaccac
      241 cgaccgcagg gtcgtgtacc gcgtcgaaga cgacatcccg gtgatgctgg cggacgaggg
      301 catcggcacg ctgcagttga ccgattttcc cggctaggcg tccggccgtc catcggacgg
      361 tggcgccgca ggtcctcgtc cgcggccggt tgcgaaagct cgttgtgaca gtttgtgacg
      421 gtgttgtgag cagcttcacg ttcccgccac cgcgacagcg catactgcag cctccctcct
      481 cgccttccaa gacgggcacc gttttccgcc atgacgccga tccccaccga tccgcccgcg
      541 gaccagcagc gcaacgcaac caccaaggtg gtcgaaatcg gttcgcgcgc taccgacgcc
      601 gaccacgacc acgtcggcgg cttgctcgcg gccgtccgcg acatgacggt caaggaagcg
      661 gcgaacctcg tcgccggcct gctcgacaac gtcgacgaca cgctgttcga cctcgccgag
      721 aaggccgaga gcaacgcgag ccagaccgaa tacttcgatg gcatgcgcga aacccgcaag
      781 cggcggccgc gcatcgagcg gctgttttcc gaacgcgtca cccgcggctt caccgacttc
      841 gcggccggtc gcgcggcacg ccccggcagc gaagcgcaaa aggcgttttc cgccagcggc
      901 gagctgagcc tcgtcgacga ccgcgaactg gaggagtcgc tcgcggtatc cagcatggtc
      961 gccaaggccg atggccggct gtcttcggca ctggtggcgc tgaaccagcg gctgtccgtg
     1021 ctgagcggcg gccgcaccac caccaacgcc aacaatccgc tcggtcccaa cgcgttgacc
     1081 gaggcgttcc gcgtcgcgct cggcgagctc accgacatca acatccgggt gcgcctgatc
     1141 atcctcaaga tgttcgagcg ctacgtgctc aacgcgctcg acgcgctcta ccacgacgtg
     1201 aacatgcggc tggtgcaggc cggcatcctg ccgcagttgc ggcgtccggc ggtcgtgcgc
     1261 gggcatggct acaccggcgg caccacgcgc gcgcaaacca ccgcgcccga cgaagcggtc
     1321 gaagctgccg acgacggcga atacgacacc gccgaccagc tccacagcga actgatgcag
     1381 cgcctcacct cgctgctggc ggcgcgccgc cacggcagcg ccggcatggg tggcgggcgc
     1441 atgtcggccg gggcgggcgc aggcatcgct tcgctcagcc cggccgaact gctcagtgcg
     1501 ttgacgctgc tgcagggcga gacgcgccca ctgccgtcgc agcccatcgc caccgacacc
     1561 aacagcgccg ccgaggtcgt ccagcatctc aaggacgcct tgatggcaca gatccagcgg
     1621 ctgtccggcg aagccaaggc gcggatttcc ggcagcgacg aggacacgat cgacctcgtc
     1681 ggcatgctgt tcgaatacat cctccaggac cacaccattc cggcgccgat gcaggtgctg
     1741 ttgtcgcggc tgcagattcc gtatctgaag gtggcgatcc tcgatcggcg gatgttcgcg
     1801 cacgcctcgc acccggcccg caaactgctg gaccagttgg ccgacgccgc caagggctgg
     1861 tcggaggagt ccgaccgcga ccatcgcctg ttcggcaagg tcaaggccgt ggtcgaaacg
     1921 ctgctgcagg atttcgacga cgacatcggc gtgttcgagc gccagctcgc ggacttcacc
     1981 cagttcgcgg agcagaaccg aaagcgcgcc gaattggccg agagccgcgc caccgaggcc
     2041 gcgcgcggcc gcgagcgcct ccaggatgcg cgtcgccgcg ctgcccagga aatcctctca
     2101 cggatcagcg ggcgcaacct gccgaccctg ctacgcggcg tgctcacgcg gccatgggcg
     2161 aattacctgg tgctgatcgt gcttcgccaa ggcgtcgaat cgcaggaatt ccgctccgcg
     2221 gtccgtttca tcgacgattt cgtcgccagc gccgacgccc cgcaaggcga ggccgcccac
     2281 gccgaataca agaacgccct gccctcgatc gaaaagcatc tgcgtaccgg cctcgccacc
     2341 gtcgccttcc aggagcccga catcgagcgg ctgctcgacg aactacggaa attctggcgc
     2401 cagcagctcg gcgagccggc acccgtcgcg cccgccgatg caccggcggt ggatgcggcg
     2461 gtgctcggca tgaaccagga cgcgcaaccg gcgatcaccg atgcggcggc cgaggcggag
     2521 gaagacttcg agtccgacga catgtcggtg gacatcgatg ccggctcgct gcaggcaatc
     2581 cgggaactca aggtgggcgg ctgggtcgag ttcatcgacg agcaaggcac ccgtgaacgc
     2641 gccaagctgt cgtggatcag ccccatcagc ggcaagtacc tgttcgtcaa ccgccgcggc
     2701 ctgaaggtcg ccgaccgcac cgccgtgcaa ctcgccaacg aattgcaggg ccagcggctg
     2761 atgatcctcg aagaggttcc gctattcgac cgcgcgctcg acgcaatcgt cgatcgcctg
     2821 cgcaacgcac acgcagagca gccgccgcag caattgcagc agcaaccgca gcaacccgag
     2881 ggatgagcgg cccggagtcg cggcagccgt tgccgccgca ggaggcgatc cgcgccgatg
     2941 tcgcgcgcgc gctcgccgag gacatcggca gcggcgacgt caccgcggca ctgatcgacg
     3001 cgggcgccat cgccagcgcg cggatcgttt cccgggaaca agccgtgctc gcggggagcg
     3061 cgtgggccga cgaatgtttc cggcaactcg atgcgggtgt gcacatcgac tggctcgccg
     3121 tcgacggtga acccattgcc cccgataccg agctgtgcca catccggggc aacgcgcgcg
     3181 cactggtcgc gggcgaacgc accgccctca acttcctgca gacactgtcg gccaccgcga
     3241 ccaccacccg cgcctacgtc gatgcggtcg cgggcacccg cgccaagatc ctcgacaccc
     3301 gcaagaccct cccgggcctg cgcctcgcac agaaatacgc ggtgcgttgc ggcggcggag
     3361 acaaccaccg aatcggcctg ttcgacgcga tcctgatcaa ggaaaaccat gtggccgccg
     3421 ccggaggcat cgccgcggcg atccatgccg cccgcgcggc gcaccccgag ttgttgctgg
     3481 aatgcgaggt ggaatcgctg gacgagctcc gcgaggccat cgacgccggc gccgaccgcg
     3541 tgctgctgga cgaattcacc gactcgatgt tgcgcgaggc ggtggcgctc gcggcggggc
     3601 gcatgccgtt ggaagtctcg ggcagcgtct atctcgagcg ggtgcgggtg atcgccgaaa
     3661 ccggcgttga cttcatttcg atcggcgcgc tgaccaaaca cgtccgtgcg atcgacctgt
     3721 cgatgcggtt cgacaacgcc tgaggcgtcg gacgtccgga tcgcgatcct gcgacggatt
     3781 cggggaagct gctttgctgt tcatgccgga gcaggccggc atccagcagc cagcgcaatc
     3841 gcggccgtgg gccgcgacag ggaaagtccg atcagggcgc cacgcgcggc gcgcgtggtg
     3901 cgcgcagcgt cagccggcga ccacctgcag ccgcacgaca cgggcacggt tcctgtaatg
     3961 ctgcgcctcg agatcttcga gcacgacatc ccaggcatcc atccccagcc atgaaggcct
     4021 caggctttcc agggtatcca tctgcgccgg atcaccctgg tgcttcagcc gcgccagata
     4081 gctcagcacc cgttcggtct ggttggcgtc gatcccgtcc atcgaacccc ctgctttgca
     4141 attgatcacg tgcatgcaaa cttcgatgca acgttcatgc cattcctgaa ccccggaatc
     4201 ggcgccgaac gcactcgatt cggaaaatgt gcgccagcgc acaggaacga attaccggcc
     4261 ggcccttccg gtcttgctga acacgggcat ggaagtcgcc ggcggcgatt cccggggcgg
     4321 ctggccgatt caaaccccgc ccagtgcagc cagcgcctgg gccatccgtt tcccggaaac
     4381 cggcccggcg gtcttcagtt cctgggcgaa caccgacacc cgccattcct cgaccagcca
     4441 gcgcagggcg tcgagcgctt ccgacttgtc gccggccgca cgcgcgtcca gcacgcgccg
     4501 ccagtacggc agcacctgtt gcaggcgtga atgatcgcgc ccggggtcgc cgcgcagttt
     4561 ctcggcgcgc aggcgcatgg ccttgaggta acgcggcagg tgcacgagcc gcgagcgcgg
     4621 cagctcgcgc aggaaccctg gcgccagcag cgcatcgagt tggccgcgca agtcggcgta
     4681 gctcgccggc gcctgcgcct tgatcgcggg cgtcatccac ggttgcaggt ccgacagcgc
     4741 gaccaggatc ggctcggcca gcttcaaccg ctccatcgcc gcgccgaaca cgccgcgcgc
     4801 aagtgcttcg tgcaacgcct gccaggccac tcgatcgcgc acatcgagat cgagcgcctc
     4861 caacagatcg acgaatcccc cctcgacgac gtcttcgcgg agcgtatcgg cacgaccgtg
     4921 cggcgcgaac gcgatcgcga gcctgggctt caatggcaac tgcttgcgcg ctcgcttgaa
     4981 ctcgccttcg agcgacagcc gcagcagccg ctcgacaccc gcgcggtgcg cggtgatcgc
     5041 ctcctgcccg gtttcgaaca cccgcagcgc aaccgcgctg cccaggtcga ccaaagccgg
     5101 atacgccggt agtccggcgc gtgtttcgga caccacggga atttcatcga agtcccacgt
     5161 gcgcacttcc tcgcgcgtca ccggcagctc ggccgcacgc gaaaacgcct tgcgcgcgcg
     5221 cgcggcccat ccggcgcgga gcgccgcgag gtcacgaccc gcggcgagcg aacgatcctg
     5281 ttcgtcgcgc acgtcgaacc gcatccgcag gtgcgcgggc agcgaggcct cgtcgagagc
     5341 cgcggaatcg atcgcgacgc cggtgatccg cttcaggaac gccgccagcg cgtgcgcgag
     5401 cggttcgtcg cgcggtggct cggcttccac gaacgcacgc gcgaagtccg gcgcaggaac
     5461 gaagttccgg cgcaacgcct tcggcaaccc gcgcagcagc gccgtgacct tgtcggccag
     5521 cagtccgggc accaaccatt cgcaacgcgc ggccgacacc gcattcaaca acgcgagcgg
     5581 cacgcgcaag gtgacaccgt cggcatcgtc gccggggacg aaccggtact cgaggtgcaa
     5641 ggcctgcccg gaaacctcca gcgcctccgg gaaagcgccg gccgcgccgg ccgcgcccgc
     5701 ttccagcacg tcatcgagcg accagcgcat ggccgcgcgt tccgaggcgg ccgcacgccg
     5761 aaaccaggca tccagtgcgg cggtcgtgga aatcatgtcg ggcacctttc ccctgaagaa
     5821 tccggcgagg tcctcgtcgg acttcacgag accctcgcgg cgctgctgcg cttcgatttc
     5881 gcgcgcttcg tccagtaccc gccgattggc gccaacgaaa tccgcgcgac tgtcgaagtc
     5941 gcagcgcgcc agcgcctcgc gcaggaagat gtcgtgggcg agcgccggat cctgccggcc
     6001 gaaggtcact gcgcggcgtt ccaccagcac caggccgtac agcgacacct gctcgaacgc
     6061 ctgcaccgaa ccgcgcgcac gcgaccagtg tggatcgctg caggtccgcc tgagcaggtg
     6121 cgccgcctgg cgctcgatcc aagtgggctc gatccgcgcg cattgcatgc cccagacctt
     6181 cgcgccgagg tcgaggatct gggccaccaa cacccacggc ggcggctttt tggagagcgc
     6241 ggagcccggg aatatcctga acttgcgctc gcgcgcgccg cgatagaggc cctgctcgtc
     6301 ctggcgcgcg acgttggtcg ggagaccggc gaggagggcg aggtggacgg gttcgtaaag
     6361 ctcgggaccg gggaccgggg accggggacc agcagctttt ccggtccctg gtccctggtc
     6421 cccggtcccc gcttcgagca acagctgcct atgcaacgcc ccccactcgc gcatccgcat
     6481 gaaggacacg aagttcttgc cacaccagtc gcgcagtttc gactgcgaga gttcctcgtg
     6541 ggcgctgcgg tacgcatccc agaggttcag taccccgatg aaatcggaac gcgggtcggc
     6601 gaatgcacgg tgggcagcgt cagcctgggc ctgcttgtcg gcgggccgct cgcggggatc
     6661 ctgggtgccg aggaaggcga cgatcggcag cacttcgcgc gccacgccga gtgtgccggc
     6721 ctcgacgatc atccgcgcga gttgcgcatc gaccggaatg cgtgcgagct gccgccccat
     6781 cggcgtcagg cgccgttcgc catcgaccgc gccgatttcc gcaagcctgc gccagccgtc
     6841 gttgacggcg cggggatccg gcggatcgat gaagggaaag gcttcgacat cgcccaggcc
     6901 gaggtcgagc atccgcagga tcacgtcgga caaggcgctg cgcaggattt ccggatcgcc
     6961 gtaaggcggg cgggcggcga aatcggcctc gtcatacaag cgcacgcaga tgccgggccc
     7021 cacccgcccg caacgccccg cgcgctggtt ggcggcggcc tgcgacacgg gctcgatgtg
     7081 caggcgctcg atctggctgc ggcgggaata gcgcttgacc cgcgcgtcgc cggaatccac
     7141 gacccagcgg atccgcggca cggtgagcga agtctcggcg acgttggtgg ccagcacaat
     7201 gcggcgcgcg ggaccgggct tgaacacccg gtcctgttcc ttgaccgaca gccgcgcata
     7261 caggggcagc acctcggtgt ggtggtactg cctgcgcgag agcgccaggt gcgcgtcgcg
     7321 gatctcgcgc tcgccgggca ggaacacgag tacgtcgcca cggggatcct cgcggctgat
     7381 gtcgtcaagg gcatcgacaa cccgttcggt gagtcctgcg tcaccctccg gggcacgcca
     7441 gcggagctcg accggaaagc cgcgcccctc gaccgtcaag actggtgccc catcgaagtg
     7501 ctcggcgaaa cgcgcggtgt cgatggtcgc cgaggtgacg atcagcttga ggtcacggcg
     7561 cctgcgcaac aggtgtttca ggtagcccag caggaaatcg atgttgaggc tgcgctcgtg
     7621 ggcctcgtcc aggatgatgg tgtcgtattc ggacaaccag cggtcgtgcg ccatctcggc
     7681 gagcaggatc ccgtcggtca tgaacttgac gagcgtatcg gtcccgagct gcctggcgaa
     7741 gcgcacctcg taaccgacga tgccgccggg ttgatcgttg tgtccgccgc ccagttcgga
     7801 agccacccgc cgcgccatcg cccgggcggc cagccgccgc ggttgggtgc aaccgatcat
     7861 cccggcgacg ccacgacccg cagccaggca cagtttcggc aactgcgtgg tcttgcccga
     7921 accggtttcg ccggcgatca ccagcacctg atgcctgcgg atcaaccgcg tgatttcgtc
     7981 cgcgcgctgc gcaatcggca gggacgcgtc caccgcgatc ggcggcacca gcgccgcacg
     8041 ggcttcgcgc cgggccgcgg aggcggcgat ttcgccggcc agcgcgccca cggttcccgc
     8101 cgagcgcggt cgctcgcgtt ccaggcgtcg caagcgtgca cgcagcttgc cggcgtcgcg
     8161 gctcagcacc gatcccatag cacgctggag attccgcagc gtcggatcgt tcgactgttt
     8221 ttccattcgc gatgccattg ccccggatga taacgagagg gatggcgtct attgcgaaag
     8281 cgcagtcgca aggtgtcgcc gccgtgcatg acggcacctg caaacgcgcg cgatttggtg
     8341 catcatcggc gttttcccaa ccggacccgc cccatgccgc gcaccacgtt gatcctcgcc
     8401 gccgccatcg catgcgcatg cgcggcctgc tcgcagcagg cccccaccgg acccgtggaa
     8461 gccgccgccg cagcgcccgc ggccaccgcg gcaaacccgg cctcggcgag cactgcgccg
     8521 gacatcggca tcgacctgtc ctacgtcgac cactcggtga aaccgggcga tgatttcttc
     8581 aaatacgcca acggcgcctg gctggagacg gccaagattc ccgccgaccg ttccagcatc
     8641 ggcgcgttct tcgacatgtc caggcgcacc gaggagcgca ccgcggacct catcgagaat
     8701 gcgggcaagg gccatccgcc cgcgggcagc aacgcgcgca agatcgccga ctactacgcg
     8761 gcctggatgg acacggacgc gatcgaacaa cacggactcg cgcccctcaa gggcgagctc
     8821 gacgccatcg atggcatcca gtcgcgtgcc gacctggccc gcgtgctggg tagccggctg
     8881 cgccaggacg tcgaccccat caacgccacc gacttccaca ccgaccacct gttcgggctg
     8941 ttcgtggccc aggggctgag cgatccgtcg cacaacatcg cctacctgct gcagggcggg
     9001 ctgaccatgc cggggcggga ctactaccgg tccgacgaca agacgatggc cggctaccgc
     9061 gaccagtaca agagatacat cgcggcaatt ttcgatgctg ccggcatcaa ggatgcgcag
     9121 gcggcggccg ggcgcgtatt cgacctggag acgaagatcg ccatggcgca ggaaagcctg
     9181 gtcgacagcg aggacgtgca cgcggccaac aacctgtggt cgctcgccga tttcggaaag
     9241 aaggcaccgg gcctcgactg gaacgcgtat ttcaaggccg ccggcctcgg tggccagaag
     9301 atcatcgacg cgtggcaacc tggcgcgatc gcgcggctgt cgaagctcac ggccgatgcg
     9361 ccgctggaat cctggaagga tctcttgacc ttccacacgc tcgaccgcca cgccgatctg
     9421 ttgccgaaga aattcgccga cctctccttc cagttccaca gccatgccct gcagggcgtg
     9481 ccgcaacagc gcgaacgttc caagcgcgcc gtggacgcca ccagcgaggc gctgggtttc
     9541 gcggtgggcc aactctacgt cgaacggtat ttcccggcat cggccaagca gcaggtcgag
     9601 caactggtgc aaaacctgat tgccgcgttc aacacgcgcc tcgagacgct ggaatggatg
     9661 acgccgtcca cccgcgagaa agcgcgggcc aagctcaaga ccctgcgcgt gcaggtcggc
     9721 tatcccgacc actggccctc ctacgacggc ctcgagatca ccgcggacga tccgctcacc
     9781 aacgaactca acgtttccag attcgaatac cggcaacgcc tcgcggtgct cggcaagccg
     9841 gtcaaccgcg accactggtg gatgaccccg caactggtca acgccatcaa cctgccgctg
     9901 gaaaacgagc tgcagttccc ggccgcgatc ctgcagccgc cgtttttcga tccgaagtcc
     9961 gatgccgcgg ccaactacgg cgcgatcggc gccgtgatcg gccacgagat cagccacagc
    10021 ttcgacaaca tgggttcgca gttcgatgcc gaggggcgct tgcacaactg gtggacgccg
    10081 gccgacgcgg aacatttcaa ggaagccacg gagcggctgg tcaagcagta cgaccagtac
    10141 gaggcgctgc cgggcctgca catcgatggc cagcagacgc tgggcgaaaa catcgccgac
    10201 gtgtcgggac tgaccatcgc ccatctggcg tatcacaagt cgcttggcgg ccagccggcg
    10261 cccgtgatcg acggcctgac cggcgaccag cgattcttcc tggcttacgg ccagacctgg
    10321 cgcgagaaga tgcgcgatgc cgcgcttcgc cagcgggtcc agaccaacgt gcatgcgccg
    10381 ggcgaattcc gcgccgaaac cgtgcgaaac ctcgacgcct ggtacaaggg cttcgacgtg
    10441 cagcccggcg ccaagctgta cctgaagccg gaccagcggg tgcagatctg gtgatcgcgg
    10501 ggaacctgcc ccggcacgca gccgtccgcg ggccggggct gtcccaccca cgctgcgaaa
    10561 agcccgacgt gctagcgtaa cccccctcgt gccgcaaccg gagtgaagcc catgttcgtt
    10621 cgtgtgtccc tctgcgcttt gctgatcggc ttcgccggcg ttgccggcgc ccagacccag
    10681 cccgcggccg ccccgcagaa agccccgccc cagcagcaac tcacaccgca gcaacgcgag
    10741 gcgctgcaga aacagaacca ggtgctgatc cagtatgccg accggatcgt cgccatgctc
    10801 gacaacggcc aggccggcca ggtctgggac caggcctccg acgtggccaa gaaatcggta
    10861 tcgcgcgacc agttcatcaa taccgtccag tcggaccacg cccggctcgg cgccatgaag
    10921 tcacgcaagc tcgcggtggt cacccacacg ctttccaagg gtgacgggaa actgccggcc
    10981 ggccagtacg tcaacatcaa ctacgccacc gagttcggca gccaggccaa gccgacccgc
    11041 gagttgctgt cgttccatct cgacagcgac cggaagtggc ggctgtcggg ctacacgctg
    11101 cacgcagctg aaaatacggt gtcgcgcaag taagccttgg aacgaacggc accggccggc
    11161 gatgccggcg cggcgctgtg catgttggta ccggtgccac gcgcgtttcc tcgcgcccgc
    11221 ggtgttcatc ccaaacgccg ccgcacgttg aggtcgcgtt cggagtgatc ctgaacctgt
    11281 tcgcaatgaa cggcgtttaa ccgcggcgtc ggctcgccgg ttgcggtacg gtgaccgcgc
    11341 agcgggatga ccgtgccttc gtcgctccgg gcgagctgcc gcgtggtgcg acccactact
    11401 ggagagttgc cgcatgtcca gaatgatcca tcgatcgctt tctctcgcta tcggcacgac
    11461 ggccgtgctc gcgttcgcgg gctgcaccaa ctacgtcaag aagaccgact tcaatgccgc
    11521 cattgcccag ctccagagca aggaccagag cctccagcag cagatcgatt cgctgtcgca
    11581 ggaaatgaag gcgggcttcg ccaagtacga caccgagatc acggccatgc agggccgcat
    11641 cagcgtgaac gacatcgtgc atttcggctt cgaccagtcg acgatcccgg agcaggccaa
    11701 gcccaagctc gacgagttcg cgaagatcat gcaggcacat cattcggacg cgctggtgac
    11761 ggtggaaggc ttcgccgacc cggcgggttc cagcgcctac aacaagcgcc tgggacaagc
    11821 gcgtgcgaaa gccgtgcgcg actacctggt caacaacgac ggcatggcgg ccgaccaggt
    11881 gcgggtcgtc agctatggcg agagccggaa ccgccaggtc aagccgggcg ccacgcatgc
    11941 cgccggcgtt cccaatcgcc gggcgacgct ggttgtggac tttgccggca gcaccgagag
    12001 ctgagacgac gctgttttcg cggcaggtgg gcccggagct ttccgggcgc acttgctcgc
    12061 gcggcgcgaa tcgcgccggt ttccataatc gctcaggcga tgcccaggcc ggccatcgcg
    12121 ccaatcgctt cttcgtcgaa gccggcaagc cgggcgaagg tgcgaccccg ctcggcgtaa
    12181 tccttgaact ggtcgaagct cggagacccg ggtgagagca gcacaacccc gcccgccgga
    12241 gcgagcgcac gtgcccgcgt caccgccaag gcgacatcgg ctgcctcatc gatcgtgcag
    12301 ccgacgcgcg cgcggcgcaa cgcatcgagg atacgcggac cggccgcacc ctgcacgatc
    12361 accgcatgcg ggggacgtgc ggcaagctcg tccgcgaacg cgccccactc caggccacgg
    12421 tcgaagccac cgatgatcag cacaatgtcc cggccgcgaa ggctctccag cgcagcgagg
    12481 gcggcgtcgg gcgtggtgct gatggaatcg tcgatccact cgatgccgtc gcgggtgccc
    12541 agtcgcgtga ggcgatgcgg caatggccgg aaggtccgca atgatcccgc ggcgccgcgt
    12601 gcatccatgc cgagcagctc gatggccgcc agcgcggcgc acgcgttgcg ggcgttgtgc
    12661 gcgccccgca agggcaggtc gtccagttcg atgattgcct cgctcccgcg atggatggcg
    12721 ccgccggcga cgtgccagcc gcaggcctcg ccgaagactg cacggtgcgg gtgcgtggcg
    12781 gttcgctcca tcagcagcgg cgatcccgcg tccaccagca gcgtgcgcga cgcgtcggcg
    12841 agcttcagct tgtcggcgac atagcgctcg cggctgccgt gccagtccag gtgctcctcc
    12901 gcgaggctga cgacaacgcc gagttccagt gggccggctt cgccggtctg gaaactggac
    12961 agctcgagcg cccacagatc cgcacgctgg ccatcgaggt ccagcaatgg aaagccgata
    13021 ttgccggcca gcgcggtacg aacgccaagc ccccgcgcga ggtgcgcaat cagtgccgtg
    13081 gtggtgctct tgcccttggt acccgtgacg gcaaccaccc gtgcatcggg gttttcgtcg
    13141 aaccagagcg cggtgcccga ggtgaaccgg gtcccgcatt cgcgcgccgc gaggatctcg
    13201 ggcttgtacg cgctgatccc gggcgacttg atcacgatgc cgtgcgaaga caacgcatcg
    13261 gcgtccggtg gccttgtccg caggtcgaga gactcggaat cgatgccctt cccctctacc
    13321 gctgaaggga aagacgtcga ctctttccgc agcgcggcag cttcgtccgg cgcgcaatac
    13381 agcgtcagcg gcttgtccgg aaaccggcga cggagcgccg ccagcacgct gcggccttcg
    13441 cgaccccagc cccacaccgc gaggtcggca cgctcaagct ccgcgaatcg catcgagcgc
    13501 ccccgagaaa cgcgttggaa tccggtgttc gccagccggt tgcagccagg gtgccagcgc
    13561 cagggtcgaa gcatcgagct gcggcagaat gtcttcgcgg aagcgcgcga ccaaggcatc
    13621 ctcgcgccac tccggccgcg cagccaaggt tgcgaacgcg gcgcgggctt cggccgcctc
    13681 gcccacgcac tcgaacggtt tgtggtcgtg gtactcgatc agcgcgtcga agccgtcagc
    13741 ctgcgtctcg tcgtccagca ggttgcgacc gaagatcgcg agcaggcgcg gcttgggcag
    13801 gaacggcgcg agcgcgagga acacgaagtg gcatttcggg cacttgccgc accagcggtc
    13861 aaccggtttt ggcccaagga tccggaagtt gcgattgcaa cttgagaata cgtcgaagta
    13921 gtccacggcc gatttggcga acgcacgagt gaccgccaat tcggaaaacg gccgcagcag
    13981 cgagaagtaa tcgaggtcgg cggcaacgcg ggagcgcaag ttcgcggcga actcgttctc
    14041 gaaatcccgg cccttgctcc attgatggtt gacctggcga ccagcgtatt cgagggtggc
    14101 ggccgaggcc gaacgttcgt tggcgaaggc aatggaatcg aagccgtaga ggaccgccgc
    14161 gcacgccagg atcgcggagt tgatcgcggt gaccgggacg tggccattcc aggcacccat
    14221 gcggttgtac tcgaacaaca ccggcgacaa ctcccggccg aggttgagca tcggcaaccc
    14281 cgtgcgtgcg gcgaccgtcg caatcagggg cgaggcgccc acccacgccg cagtcgcgcc
    14341 gacgccgatc gatttcaacg actcgaccac gaccagcgaa tccttgccgc caccgatagg
    14401 taccagcgtg cgatgcggca aatcgagcgt cgccgcttcg ccagcgccag gaacggcaaa
    14461 ggcactggat cccggcctgc gccggggtga cgataccggg aggtcgttcg caagtggcgc
    14521 gccgcgcgga aaagcgatcc gcccgtcgag gtcgatacca ttgcgatagg cgaactcggc
    14581 gagcccgtgc cggtacagcg tgtcgagcag gtcggccaac gccgcatcca atgcgccgtt
    14641 ctcgacttgg atcgcgggcg gaatgccggc cttgtagtag ctcactccgg ctaccaggtg
    14701 cagcaaccgc aaagccgcat cgaatgcctg gcggcgctcc tcctgcaacg gcggcgcatc
    14761 ggggaaacga atgcgctcga ccagatccgg tccctcgtcg aacgcgtaca ccagctccgc
    14821 cacgccatcg tcgtacccgc agcgcacgaa ccggaacctc tccgcctggc gcgggtcgac
    14881 gagcatgttc attcgatcac caccgattcg cccagcgagc gcaggttgta gtgggatgcc
    14941 atggattcgc cataggcgcc ggcctgtgcg atcagcaaca cgtcgccctc gcggctctcg
    15001 ggcaggtgcc ggtcggcgcc gagtacgtcc ccggattcgc agatcggacc gaccacctgg
    15061 tacagcgtat cggccggcgc gtcgatccgg gtgaggttca cgatttcgtg ccacgcgtcg
    15121 tacagcgcgg ggcggatcag cgcgtgcatg ccggcgtcgc agccgagata gttgagcgcg
    15181 cccttgcgct tggtttgggt gacacgcgcc agcagcaccc ccgcgtccgc caccagccag
    15241 cggccgggtt cgagccacag ttcgtattgc gggcaggtcc gccggacttc cgccagcgcg
    15301 gagtcgagaa catccaggtc gagttccgcc tcccccggat gcgacggcac gccgaggccg
    15361 ccaccgaggt tgaggcaccg gacggtgccg atgcggtcgg cgatgccggc gagctggctg
    15421 cacaccagcg cccaatgcga cgcatccagg atcccggagc cgagatgcgc atgcacgccg
    15481 gccacgcgaa ccccgagtcc gcgggccagg gcgaggaagg cgtcgagctc ctccagcggc
    15541 agtccgaact tgctggtcga gccgccggtg cgcaccttgt cgtggtgccc tgccccgcgg
    15601 cccaggtcga tgcggagcat gatttcgcgg ccgcggaaaa tcccgggcca atgctgcagc
    15661 ggatggaggg cgtcaagggt caggaataat tcaggactcg ggacgcggga cttgggactg
    15721 ggagaagaac cgctccggac tgcaaccgcc ccgccttgat tctgcaatgc ctccgcgtag
    15781 tcgctgcgcg gcgcgaagtt gggggtaaac aggcgcgacg attcgcgcgc ttccccgatc
    15841 gcctcgaccg caacgagttc gcccggcgag acgcattcga aacccaggcc ttccttggcg
    15901 agaaccgcca ggatcgcggg atgcgcattg gctttcaccg cgtagtagac gcgatcgatc
    15961 gactgcaaac cccgcaacgt ccgcgcgcgt tcgcggatcg tcggcagatg gtaaacgtag
    16021 cgcggcgtcg tgactttttc cgctctcccc tcgggagagg gtgcccgact acggccgacg
    16081 ggatcggccg tgaacggcat ttgccggccg gaagggcgag cgccatggat ggcgcgagta
    16141 agggcgggtg agggttcggg atccgcgagg tgattcaatt cagtcgaacc ctcacctcct
    16201 gtccccctct tggcgggaga ggaaggactt ccggcgagtt cgagcaacgt ttcccgcgcg
    16261 ccctgccacc acggcgcacg cgtgcgcgcg ccgccgccgc catagagcgc ctgccaggcc
    16321 gggccgaaca gcccggcatc gtcggttcgc aacgcgcctg cgcggatcag cagttcgtgc
    16381 aggcgcagcg tcaggccgtc cagcatgccc tcgtcgacca cgaaggtgag gttgaggttg
    16441 ttggacgact gcgaaatcag gtgtacgcgt tcgcgcccga actcggccaa cacccccgac
    16501 aggcgatgca gcatcgagcg catcccgcgc ccgaccagcg tgaccgccgc acacggcgag
    16561 atcaccttga cccggcagac cctggcgaga tccgccgcga gcgatgccac cacatcggaa
    16621 tcgagcaggt tctcggccgg atcgagcgag acggtgacgt tggtttcggc ggagccgacc
    16681 aggtccaccg acaggccgtg ctgcttgaac aaggcgaaca cgtcggcgag gaaccccacc
    16741 tgctgccaca tcccggaagt ttccatcgac accagcgtga tgccacgtcg cgaactgatc
    16801 gccttgatgc tgggcacggc ctccgcgggc tgcgggccga tcaccgtgcc ctcgagatcc
    16861 ggctggcggg tgtccttgat ccacaacggc acccgcgcgc gccggcacgg cgacaggcag
    16921 cgcgggtgca gcaccttggc gccggtggtg gcgatttcct gcgcttcctc gtaatcgagg
    16981 tgtcgcagca ggcgcgcgac aggcacctgc cgcggatcgg cggtgaacat gccggcgacg
    17041 tcggtccaga tctccacccg tgccgcgcgc agcaacgccc ccatgtacgc ggccgaggtg
    17101 tcggagccgc cacgaccgag cagcacggtg gccccggatg cgtcgcgcgc gatgaagccc
    17161 tgggtgatga acacctcgcc gcgggcggcg agtgcggccg cgaaagccgg atccggttcg
    17221 gcctgtactt gggccgacag catgcgcgcg cgctcgttga ggaacggcag ctcgcgcgcc
    17281 tgcaatgcgt cccgcgcatc cagccattcg gtcgcgaggc cattcgccgt catgaacgcc
    17341 gcgccgagcg aactcgacag caattcgccg tgggcctgca tcgccgcatg ccacgcatag
    17401 cccctctccc ctcgggagag gggttggggt gagggtacgg ggtctttcaa cgatgggcga
    17461 atgcccgaac cctcacccgg cgctgacgcg ccaccctctc ccgaagggag agggaaagcc
    17521 aaagcctgca aagacgcgag ccagcgttcc atcgccgcgg tattcgccaa ttccatctgc
    17581 gccgcgagtt ccctgtgccg cgcgaccagc ttcaccaacg cgccttcgcg ttcgtcggcc
    17641 cggcaggcgc acaatgcctt cagttcgtcg gtgacacccg cgagcgcaga caccaccacg
    17701 agcacgcgca agccttcggc gcggcgcgca gccgcgaggc gcaggatcgt gtgccagcgg
    17761 tcgacggtcg ccacgctggt cccgccgaat ttcagcacga cccaaggcgc gtcgggcggc
    17821 agcggcgcag ggttcgatgc ggtcatcagg gacggtgcgg caacgggcgc gcggcggcgc
    17881 ggacaaggcc cgcaagtgtt gcgtgcaggc gtggaaaacg caatcgcgca ggctcccgcc
    17941 ggtcgtcaag tcgctgttca gagccctgcc ggcaagcacc caatacaatc gtccgatgca
    18001 gcagacaccc cagaacccgc cggcctcgat cgtgccggag ccgatccgcc gcggcatcga
    18061 tctccggatc aacggcaaac agttcagcca taccggcgat tccgacctgc cgctcctgtg
    18121 gtacctgcgc gacgtgctgc gcctgaccgg ctgcaagtac ggatgcgacg acgcgagttg
    18181 cggcgcgtgc agcgtgctgg ttgacggcaa gctcgagcat gcctgcacgc tggcgatgca
    18241 ggatctggac ggccgcgagg tgaccacggt cgaggggctg gccggcccgg atggcgcgtt
    18301 gcatccggtg cagcaggcat ggatcgacga ggacgcgatc ctgtgcggct actgccaacc
    18361 gggacagatc atggcggcca tggacctcct gtcgcgcaag ccaaaaccct ccgacaagga
    18421 catcgacggc atcgggaacc tgtgtcgctg cggcagttat ccgcgcatcc ggcgcgcgat
    18481 caagcgcgcc tcgaaagcca tgcaaggcaa ggccaagtga agcggccgca cgacgccgga
    18541 cggcgccgct tcctgaaagt ggtcggcgcc gcgaccggag ggctggtggt gggcctgccc
    18601 gcattcggct gggtgcccga cgaattgctc ggccagaacc tggtacgcct gggtccgtac
    18661 gtccgcatcg agcccgacgg caccaccgtg atcggctcgc gcgacccgga agtcggacag
    18721 ggcatccgca ccgccgaagc gcgcatcctc gccgaggaac tggacgccga ctggaccagg
    18781 gtggtcgtgg aaccgctgga cctcggcgtc accgccgtcg gaggcgagcc gcactggacg
    18841 ctcggtcacc aggtggcttc cgggagcacc agcattcccg cggcctggaa cgaccttcgc
    18901 atcgtcggcg ccgccgcgcg tgaactgctg gcgcgcgccg cggccgaacg ctggaagacc
    18961 accatcggga acgtgcggac ggtgcgcggc gtggcgatcg ccgccgacgg ccgccggctc
    19021 ggctacggcg acctggcgca cgcggcatcg caactccaag cccccgctgc agcgccggcg
    19081 cctaagctgc cgtcacagta cgtattggtc ggacaggatg cgggcgacgt cgatgcgcgc
    19141 gacatcgtga ccggcgtgca gcgctacgcg atcgacgaat ggttcggcga cgtgctggtg
    19201 gcggtgatca cgcgctgccc ctggcccggt ggcgggctcg cgcgcctcga cgaccgcgcc
    19261 gccctgaagg tcaagggcgt ggaaaaggtc atgacgatcc cggcgcccga tcccgcccgc
    19321 gcaccgggca cgcagcaact ggcggccggc atcgcggtgc tggccacgga cacctggtcc
    19381 gcgttgcagg gcgtcgccaa gctggacgtg gaatggcagc ccggccaggc agtgcccgaa
    19441 ggcgacggcg cgctcgggaa gtcggccgaa gcaacgatgg ccggcgatcc caccaaggcg
    19501 gtgcgcacgg acggcgactt cgcggaggcc ctcaagagcg cgcgctccac cttcaaggcc
    19561 gaataccgca tcgcgacggt tgcgcacgcc gatctcgagc cccccggcgc gctggtcagg
    19621 gtcgaccacc agcaggcatt gatcatcgcg ccggtgcaga atccgcgtgc cacctacgaa
    19681 accgtgcgtc gcctgaccgg cctcgccccg gacaagatcg atgtgcggct tccgcgtgcc
    19741 ggcggcggct tcggacgctt cctcgaaagc gactacgtcg ccgaagcggt gatgctggcc
    19801 aaggccgctg gcaaggcgat caagttgatg tggacgcgtg gcgatgcatt cacccacgac
    19861 ctctaccggc cgttttcggt gcacgcattg gaagcctgcg ccgaccgcca caaggcattg
    19921 accggctgga gccaccacat cgccagcacc tcgcgcctcg cgggccgcga gccgcgttcg
    19981 cgctggtggc tttcggaaat gcatccggac gaccttcccg ccggcctggt cgacaacctg
    20041 caatacgcct ggtacgccct cgattccacc ctcccgcgcg gcaactaccg cgcgagctcg
    20101 cacgcggtga acgcattcgc gaccgagtgc ttcctcgacg agattgcgca gacgctcaag
    20161 caggatgcac tcgagttcag gttggcgctg ctcggcgaac cgcgcaagct cccgtatcgc
    20221 ggccacggcg gcccggtgct cgacaccggt cgtctggcct gggtgctgaa gctcgccgcc
    20281 gatgccattg gctggagcaa accgcacacc cagggccacg gcttcgggct ggcctgccat
    20341 ttcaccttcg gcggctacgt cgcacacgcg atggaggtgt cgatggaagg caaccggctg
    20401 ctgatccaca acgcggtgtg tgccgcggac atcgggcgcg tcgtcaaccc gctcggtgcc
    20461 cgcgctgcgc tggtcggagc gaccctcgat gggctatcca cggccttgcg cgagcggatc
    20521 accgtcggca aggacgggcg catcgcgcag cagggcttca aggattatcc gctcgcgacg
    20581 atggcccaga tgccgcacga cgtgcaggta atcctcgtac cctcgcagga gagtccggct
    20641 ggtgcattcg cgatgggatt tccctccgcc gcaccggcac tggccaatgc gattttcgcc
    20701 ggcaccacag tgcgcgtcca ccagatgccg atctggccgg aactgatgcg gctcttgtag
    20761 gagcgcgaca ggcacgcgac aggcatcaca ccgcgaacga atcgatcgcg gccacggacc
    20821 atcgagtcgt aggttgcgtt gaccccgggc ttgtcccggg ggaagcccaa cgtggacccg
    20881 ctgcgagcat tggcttcgcc ggacggagcc cggctcaacg caacccgcac catctgaatc
    20941 atcggccgtc cacaaagccg cgcaacacct tgaacgtgcc cgccgcatcg atttctccga
    21001 tcgcgagcag gccaccgtcc ggcgcgtacg cgcgacgccg acccggcgcg gcggtcacac
    21061 gcaacgcgcg gccatgcgcg acatcgtcgg cgccccgctc gtcgagttgc aacgtgggcc
    21121 agtcggcaag gcccgcatcg atcggaaaga ggcattcgtg cagtgccgcc ggatcccgat
    21181 cgcgcaacga ttccaattct gcgagggtga acatgcgcgg atcgcgaaac ggctccaccc
    21241 acagccgccg cagtgacgtc aggtgcgcgc cgcaacccag cgcctctccg aggtcgcgca
    21301 ccaggctgcg cacgtaggtg cccgagccgc attcgacata caggcgcagc gtgttggccg
    21361 accggccggt gagatcgaat cggaacacgt ccacctcacg cggcgccgcc tccacggctt
    21421 cgccgcggcg ggcacgtttg tagagcggca ccccgccctg cttcagcgcg gaatagatcg
    21481 gcggaacctg ggtgatgcgg cccgtgagct tcgcgagctc gcggctgatc gcgttgtctt
    21541 ccagcgtcgg aacctcgcgt tcaagcaaca ccgtaccgtc gctgtcgcag gtgtcggtgg
    21601 tggtaccgag ccggcattcc gcggcatacg ccttgcgcgc cccgagcagg taacccgcga
    21661 ccttggtcgc ctcgccgaaa cagaccggca agagtcccgt cgccagcgga tcgaggcttc
    21721 cggtgtgccc ggccttgctc gcaccgaaca gctgcttcgc ctgctgcaag gcgcggttgg
    21781 agctgatgcc cgtgggcttg tcgaggagga ggatgcccgt gactggggac cgggaagagc
    21841 tttcggccga tgtcatcggc tcaccccaag gccgcgatca acgccaactg acgacaacga
    21901 aagatgggtt gccccggaaa cgtcgaagat ctgtaccagc ggatccatgg gcggaaagac
    21961 tcgaacaaat cacgatgacg aggcgctctg gtccccggtc cctggtcccc ggtccccttc
    22021 ttcccgcagc agccgctcga tccgctcgcc cttgtccacc gagtcgtcgt acttgaagcg
    22081 cagctcgggg acgcggcgca tccgcatggt gtgcgcgagc tggtggcgga attccctggc
    22141 gagcgccttc agcgccttga cgacttccgc cgatcgctcg ggctgcaatg ccgtgaccca
    22201 gaccgtggcg acgtcgagtt cacggctgac gtcgacatcc accacgctcg cctgcggcag
    22261 cgcgtggtcg cgcaccgcgg cgtgcaccag cacgccgacc tcgcgacgga gctcggcgtt
    22321 gacacggtcg gtccgatgga aggagcgggt gggcatcaga ggactccgga aagcccttct
    22381 ctccaagaga aggtggcgcg aagcgccgga tgagggtacg ggtctcgcgc gagactgcac
    22441 aaatctggca acgccgaacc ctgaccccgg cgttgcgcgc tcggcctgcg gccgcctctc
    22501 ccgagggaga ggggctcgaa tcacagcgtc cgcgccacct cgatgcgttc gaagcattcg
    22561 atgcggtcgc cgggcttgac gtcgttgtac tgcttgacgg cgatgccgca ttcgctgccg
    22621 ttgcgcactt cctcgacgtt ctccttgaag tgacgcagcg actccagctc gccctcgaag
    22681 atcaccacgt cgtcgcgcag cacccggata ggcttgttgc gcctgacggt gccttcgatg
    22741 accatgcagc cggcgaccgc gccgaacttg gagctgcgga acacatcgcg cacttcggcc
    22801 acgccgagga tctcctcgcg cacctcggtg cccatcagcc cgctggc
//
LOCUS       DASSNH010000235        15873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_776240, whole genome shotgun sequence.
ACCESSION   DASSNH010000235 DASSNH010000000
VERSION     DASSNH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 15873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15873
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776240"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFK00_08525"
     CDS             complement(<1..951)
                     /locus_tag="VFK00_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7593032.1"
                     /translation="MNSHHRLYGRTLLVALACVFAASLAEGQTTLPQVVVTATRTAQT
                     QDATLSPVTVITREEIERLQPSSLQDLLNASPGMAISNNGGPGKQSSLFLRGTDGDQV
                     LVLVDGIRIGSASAGRTAIQDIPVDQIQRIEIVRGPFSSLYGSEAIGGVIQIFLRHAP
                     GTFTPNASVGFGSDSHWKGAAGFSAGGDKGWVSAQASHERTDGINSCRVGAAEAFAGC
                     FADQPDRDGFRDDALSVHGAYEFNEQWSADGLAFRSQGRNEYDGTFSDADDYSNQVLV
                     LVDGIRIGSASAGRTAIQDIPVDQIQRIEIVRGPFSSLYGS"
     regulatory      complement(1223..1481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1753..4149
                     /locus_tag="VFK00_08530"
     CDS             1753..4149
                     /locus_tag="VFK00_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7593033.1"
                     /translation="MHKRNLCIAVAFACAAFASSTAVAQDTGANNANDDQKQPATLQT
                     VIVTGTRAFDRTAADSLAPIDVLTPKELVSTGAPDLTTALRTLLPSFNFPQPSLTDGT
                     DSTMPAQLRGLAPDQTLVLINGKRQHTTAILNVNGSIGRGSSPVDLSAIPINAIQRIE
                     VLRDGAAAQYGSDAIAGVINIILKGGADHGGANVSYGQYDGGQGNTWNGGADGGIALG
                     NNGWVHLSANYLHQDRTSHAGPDTRYPDDPTYGTVTFRFGLPETIAKQAALNAQYNLG
                     ENATLYAFSVFNKRNVVAGGFFRSLSQYETSTPAAAAVYPDGYLPLEHSAILDDTTVL
                     GIKGDAGGWHYDLSGDTGGSHWKLHTTDTFNYSLDAESPTAFYIGTLTIRQNEFNADF
                     SRDFDTGWYHPLTVAWGLAYRHEEFAIKEGDPASYAGAGAQVYPGYQPGDAGSHSRSN
                     TAGYVDLETDFSERFSAGFAARYEHYSDFGNTTSWKLSGRYAFTPVVALRGTISTGFR
                     APSLQQEYYSSTSINFVNDGSGNLVPYTIRTFPVSDPAAVALGAQPLKPEKSHNYGIG
                     LVFTPGTGPYATLDLYQVDIDDRIILSGNLVGTPVQDYLTSVGIPFVSGGRFFTNAID
                     TRTRGADLVGTWPVQLQGSTLKFTGGINWNKTDIRSIKPNPPQLGLAGLVLPIIDRAE
                     EGRITVGTPRTKAFFAADWGIGNWTLHGQLTNYGHWESLGGTPSGDQTYGSRTLLDAS
                     IRYALDRWDFSLGGNNVTNEYPEKNNDANNYHGIIAYPLTSPFGFNGSYWYASAAYRW
                     "
     gene            complement(4302..6002)
                     /locus_tag="VFK00_08535"
     CDS             complement(4302..6002)
                     /locus_tag="VFK00_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593034.1"
                     /translation="MSTTTGTDACGCREAAMLRISRVILVALCLMGTSAASAGLQHEH
                     PGTIAPEGVIGHVHFPVSCPGEQQAFDQATTLLHSFWYEKAEATFAGIARRDPRCAMA
                     WWGEALSLWHPLWPDRPDSQVLARGRALLAQARAAKQQTQQETAWLDALAAFYGPDGS
                     TDYRERALAYEQAMARVHAGSPREPEAAAFYALALLASAQASPPDKSHARERKAADLL
                     NRVLAVEPDHPGALHYLIHAYDSPSLAKYALGAARSYAHIAPAVPHAQHMPSHIFTRL
                     GLWNESVASNRAAESAAKHFAAEMRLPGAWDEQLHAMDYLEYAYLQLARHRDALSVQD
                     ELLAIPVASPANFKVAYAYAAIPARYAIERRQWREAADLPAHHPEFPWARFPAAQAIT
                     EFARALGAARSGDLPRATRSVGRLASLEDALRQSGDAYWASQVEIQRLAAQAWLAHAQ
                     GRPGKALAAMRQAADLEDATEKRPVTPGPLVPARELLGDLLLELDRPAEALDAFRIAL
                     AESPSRFNSLAGAACAARRLGGQDTANRYARQLPRADARSRGGRFELARGKSTPTCNR
                     "
     gene            complement(6097..8694)
                     /gene="clpB"
                     /locus_tag="VFK00_08540"
     CDS             complement(6097..8694)
                     /gene="clpB"
                     /locus_tag="VFK00_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET7593035.1"
                     /translation="MRTDKLTSRFQQALADAQSIAVGRDHNMLEPAHVLAALLDQQGG
                     STAPMLAQAGVNVPLLRQRVGEIMERLPSVRGQEGNINVSNELNRLLNLTDKLAQQRG
                     DQFIASELFLLACLEDKGDIGRALQAAGANKQNIDAAIEKLRGGEKVQSEGAEDQRQA
                     LEKYTIDMTARAETGKLDPVIGRDEEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVKDEVPEGLKNKRLLALDLGALIAGAKFRGEFEERLKGVLNDLAKQEGQVILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KVFVGEPNVEDTIAILRGLKERYAVHHGVEITDPAIVAAATLSNRYITDRQLPDKAID
                     LMDEAASRIRMEIDSKPEELDRLERRLIQLKIQREMLKKEKDLESKKRLEDLETDIER
                     LEREFSDLNEVWKSEKATLQGDAKIKEEIEKAHLELEAAQRRQDYARMSEIQYGQLPA
                     LEKQLAAAQNTDQREFKLVKDKVTAEEIAEVVSRWTGIPVSKMLEGEREKLLQMESAL
                     HKNVIGQDEAVKAVSDAIRRARAGLSDPNRPYGSFLFLGPTGVGKTELCKALAGFLFD
                     TAEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNLGSQQIQEIASSGIDEDSPEA
                     YTQMKAAVMGVVQAHFRPEFINRLDEIVVFHPLDKSQIREIARLQTNYLARRLQERQI
                     DLEISDGALMLLGNVGFDPVYGARPLKRAIQAQLENPLAQKILAGEFVGGDTIRVDAE
                     GGKLVFHKR"
     gene            complement(8874..12119)
                     /locus_tag="VFK00_08545"
     CDS             complement(8874..12119)
                     /locus_tag="VFK00_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7593036.1"
                     /translation="MPIERKSPRRFAERSDSHAATQRARGSAARDDGGQLYRALFANA
                     PTPFLLLAPDAPRFTVVDVNEAYLAATMTTREGIVGRGLFEAFPDNPDDPAATGVRNL
                     RASLERALSSGRTDAMPRQKYDIARPDGVFEERWWDPYNTPVLSPDGSVAAIIHHVTD
                     ATGLELAEIALRQSEERKAFLLRLSDALRPLADPVAIQEAASRLTSEHLDIGRVAYCE
                     VRSEPDIMVIFERDWPRRGMPSVAAGRYRLEDLGKFLVEELGAGRDLSIADVATDSRF
                     SPAERSAWEAFSIQTACSIPLIKEGRFVACVTAQDNRPHAWTDAEVTLLRDVSERTWS
                     AVERARAEAALRTNEAWLAGQKAALQAAVNDEPLAVSLGVLVRVACARDPELRCAFYL
                     ANPSRTALHHVVGMGEDHARRMAGLAIGAGSLACGLATHLGQPVITPDVTEEPLWEPW
                     LALAEAFDFRACWSFPIETEGGKAVGTFAMYYRRPREAKPGDHHLAAIVTRTAAIIIS
                     RHQATKARARAEASLRKREERQAFLLKLSDALRAEADPVNVGAIGTRMLAEHMGVERS
                     YICEFSRERDLAWIGPEYHAPNIPPIAGQYRFRDFPESMRRLETEPLVISDLCNDPSF
                     PERDKRSIGAMGMNAVLTGILRKGERNYVWALVVGDSKARDWSAEDRSLVEAAAERIW
                     TAMEHARVEDALRENQDRLQQASRIKDEFIAMLGHELRNPLAPIATTLQLMKLRAPDV
                     FVREREIMEAQVRYLTGLVDDLLDVARIARGKVELEKEPLAMAEVVAAAVETTQPAME
                     EHRQSLDTQVEGDPMVMGDRRRLVQVLVNLLGNAAKYSLPGRSIDLRVRAEGGQVVVR
                     VRDQGRGIAPELLSRVFESFTQERQAIDRAGGGLGLGLAIVRNLVAMHGGSVEALSEG
                     HGKGSEFVVRLPLLPWRPASGEAAAVANVRFPRTPPAEADGAGGKVLIVDDFAVAAES
                     LALLLQEMGYRTRVVYDGAAALQAFAEFEPDIALIDIGLPVMDGYEVARSVRGTPGRE
                     HLPLIAITGYGQANDHVRVMEAGFDEHLVKPLRAGKLGPLIEKLVERGRRA"
     gene            complement(12227..14818)
                     /locus_tag="VFK00_08550"
     CDS             complement(12227..14818)
                     /locus_tag="VFK00_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7593037.1"
                     /translation="MADERESPDDSRPAGRGEGMRALIHAHDWSATPLGPMDGWPQTL
                     GTAVELILPNRFPSVVLWGRDLIQIYNDSFRPLMADKHPAGLGQPARECWPEVWHIAG
                     PIYDRVWQGESLMFEDALYPVLRNGAINDAWYDVCYSPIRDETGSIQGVLVTVIEVTA
                     RHQARLQQQRNDRVTSRLAAIVESSDVAIISKDLDGVIQTWNAGAEHIFGYTAEEAIG
                     KPVTMLMPPGREDEEQAILARIRRGERIDHYEAVRRRKDGTLIDVALTISPINDGDGK
                     PVGASKMARDISQRTRAGAALREAEERRAFLLKLSDAIRSLTDARAIQQAACGIVRAQ
                     LAADRVCCCETDQQEESILVTAEDGAPGDTVLVGQRLRLADFQDSASAMFRELRPVGC
                     TDLDTLAELSPEQKRAFAALGIRAYLAVPAVKEGRLVGAMIAKFATPHQWSAREFQLL
                     GETAERTWDAARRASTEAALRESEAQLQRASRAKDEFIAMLGHELRNPLAPIATTLEL
                     MKLRGPDLFVREREIIDAQVGHLTALVDDLLDVARIARGKVELQTAEVDLAAVVAAAV
                     EITQPLMEERQQSLCVLVAEGLAVSGDKRRLAQVVVNLLTNAAKYSPLGRKIEVRATR
                     EDRKVVLRVRDEGQGIDPALLPHVFELFTQAAQSADRQTGGLGLGLAIVRNLVTLHGG
                     TVVAASEGRGKGSEFTVRLPLVARRRHRGASKAVTGASHPAGHRTKAKVLVVDDYALA
                     AESLALLLGEMGYVTRVAQDGPSGLQAVAEFDPDIALVDIGLPVMDGYEVARAVRRTP
                     GREDLPLVAVTGFGQASDHARAKEAGFDEHLVKPLAAGKIGQLIDTLVEARTRERTSG
                     ERRLKPA"
     gene            14961..15752
                     /gene="otsB"
                     /locus_tag="VFK00_08555"
     CDS             14961..15752
                     /gene="otsB"
                     /locus_tag="VFK00_08555"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7593038.1"
                     /translation="MQPTFRQAPTARDADIGPLPAPPAPGGDDAWALFLDVDGTLLDF
                     ARHPDEVEVAEHLHEDLDRLRVRLQGAVALLSGRSLAQLDSLFDWRAHAAGGLHGAEL
                     RTPDGEQHFTGDEDSFAQVRARAARLVDEVPGLVLEDKLRALALHYRHAPGARQAAER
                     VAQALLRDTGDRYVAQRGDQVIELKPAGVDKGRALEALMQVVPFRGRRPWMLGDDLTD
                     EDAFHRVNAMGGTSVIIGDRRPTAARCAIASPAAVRAWLHALAGD"
BASE COUNT         2493 a         5561 c         5158 g         2661 t
ORIGIN      
        1 cgaaccgtac aggctggaga acggcccgcg cacgatctcg atgcgctgga tctggtcgac
       61 ggggatgtcc tggatcgcgg tccgcccggc gctcgccgaa ccgatgcgga tcccgtccac
      121 cagcaccagc acctggttcg agtaatcgtc cgcatcactg aaggtgccgt cgtattcatt
      181 gcggccctgg ctgcgaaacg ccaggccgtc ggcgctccat tgctcgttga actcgtacgc
      241 gccatgcacg ctcagcgcgt cgtcccggaa tccgtcgcgg tcgggctggt cggcgaagca
      301 accggcgaac gcttcggcgg caccgacccg gcacgaattg atgccgtccg tccgctcgtg
      361 cgaggcctgc gccgagaccc agcccttgtc gccgcccgcg gaaaaaccgg cggcgccctt
      421 ccagtgcgaa tcgctgccga agcccacgct cgcgttgggc gtgaacgtac cgggcgcgtg
      481 gcgcaggaag atctggatca cgccgccgat cgcctccgaa ccgtacaggc tggagaacgg
      541 cccgcgcacg atctcgatgc gctggatctg gtcgacgggg atgtcctgga tcgcggtccg
      601 cccggcgctc gccgaaccga tgcggatccc gtccaccagc accagcacct ggtcgccgtc
      661 ggtgccgcgc aggaagagcg aggattgctt gcccggtccg ccgttgttgc tgatcgccat
      721 gccgggactg gcgttcaaca gatcctgcag cgacgacggc tgcaggcgtt cgatctcctc
      781 gcgggtgatc acggtgaccg gggagagcgt cgcatcctgg gtctgggcgg tgcgggtcgc
      841 ggtgacgacg acctgtggga gggtcgtctg gccttcggcc agtgacgcag cgaaaacgca
      901 ggccagcgcg accagcagcg tgcggccgta caaacgatga tgggaattca cgaagcactc
      961 ctgtccgcgc ctgcccgcgc ggtgtcgatg gcgtcgggga caggcggagg gatgctggta
     1021 ccggcacgga atgcgcggcc ccagatacaa gcccaccgct tgtccggcta aggagcatgg
     1081 gccgcagaaa tcttccggcc tgcaacagtg cctgcgcggt ccagctttcc gccgatccct
     1141 ggcccatgga accaccatgg gccggcgaat cgtcgaaaac ctgtccttac gcagtcactg
     1201 tttcgtctcg aaagtttctg cggtccaagc tccagccgaa ggccggtctc cgggctcgcg
     1261 agcgaaggtg tcgccacctt cccgatccgc gccttcccat gctcgcgcat agtggctgcg
     1321 tgcggatcgt gactcgccta ccgttgcggg ggcagctccg gaatggccgt ccatttgcgt
     1381 gcagcacgcc aaatggcgca cgctgggtgg atcaggcgtc accggattcc cgtttcaacc
     1441 gccgacgtgc gtcgacggtc acctcaggca gcacgcatca tacggcaaca tggactgccg
     1501 gtgtgcaccg cggttttgcg ggcgattccg atcgagggcg ccgcgatcct cgcccgccga
     1561 tgccgcgctc gaacccgcgg gggcgcgctc ggtgcgcacc gtacgcgcac atgccgggca
     1621 ggtgcaggtt tgacaatctt cggctcgaag cctagcctgc ccggcagaag ccgcgtgtag
     1681 ccgacgtctg cggacgtcac gggagggttg cgccgggtga agtttcctcc atccacacct
     1741 ggggacccca gcatgcacaa gcgcaatctc tgcatcgcgg tggcatttgc ctgcgccgcc
     1801 ttcgcaagct ccactgctgt cgcacaggac accggcgcca acaatgccaa cgatgaccag
     1861 aagcagcctg cgacgctgca gaccgtcatc gtcaccggca cccgcgcctt cgaccgcacg
     1921 gcggccgact cgctggcgcc gatcgatgtg ctgacgccga aggaactggt gagtaccggc
     1981 gcgcccgacc tcaccacggc gctgcgaacg ctgctgccct ccttcaactt cccgcagccg
     2041 tcattgaccg acggcaccga ctcgaccatg ccggcccagt tgcgcggact cgcccccgac
     2101 cagacgctgg ttctgatcaa cggcaagcga cagcacacca ccgccatcct caacgtcaac
     2161 ggctcgatcg gacgcggatc gtcgcccgtg gacctgtccg cgatcccgat caacgccatc
     2221 cagcggatcg aggtgctgcg cgacggcgcg gcggcgcaat acggttccga tgccatcgcc
     2281 ggcgtcatca acatcatcct gaagggcggc gccgaccacg gcggcgcgaa cgtcagctac
     2341 ggccaatacg acggcggcca gggcaatacc tggaacggcg gcgccgacgg cggcatcgca
     2401 ctcggcaaca acggctgggt gcacctgtcg gccaattacc tgcaccagga ccggaccagc
     2461 catgcgggac cggacacccg ctacccggat gatccgacct acggcaccgt gaccttccgt
     2521 ttcggcctgc ccgaaaccat cgccaagcag gccgcgctca acgcccagta caacctcggc
     2581 gagaacgcca ccctgtacgc cttcagcgtg ttcaacaagc gcaacgtcgt ggccggcgga
     2641 ttcttccgct cgctgtcgca gtatgaaacc agcacgccgg ccgcggccgc agtgtatccg
     2701 gacggctacc tgccgctcga acacagcgcg atcctcgacg acacgacggt gctcggcatc
     2761 aagggtgatg ccggcggctg gcactacgac ctcagcggcg acaccggcgg cagccactgg
     2821 aagctgcaca ccaccgacac cttcaactac tcgctagacg ccgagtcgcc taccgcgttc
     2881 tacatcggca cgctgacgat ccgccagaac gaattcaatg ccgacttcag ccgcgacttc
     2941 gacacgggct ggtaccaccc gctcacggtc gcctggggcc tcgcctatcg ccacgaggag
     3001 ttcgcgatca aggaaggcga tcccgcgtcc tacgccggcg ccggggcgca ggtgtatccg
     3061 ggctaccagc cgggcgacgc aggttcgcat tcgcgcagca acaccgccgg ctacgtcgac
     3121 ctcgagaccg atttcagcga acggttctcg gccggcttcg cggcccgcta cgagcactac
     3181 agcgacttcg gcaacaccac ctcgtggaag ctgtcggggc gctatgcgtt cacgccggtg
     3241 gtggcgttgc gcggcacgat ctcgaccggc ttccgggcgc cgtcgctgca gcaggagtac
     3301 tactccagca cctcgatcaa cttcgtcaac gatggcagcg gcaatctggt gccctacacc
     3361 atccgcacct tcccggtgtc ggaccctgcg gcggtggcgc tgggcgcgca gccgctgaag
     3421 ccggagaagt cgcacaacta cggcatcggc ctggtgttca cgccgggcac cggcccgtac
     3481 gcgacactgg acctgtacca ggtcgacatc gacgaccgca tcatcctatc cggcaacctc
     3541 gtcggcacgc cggtgcagga ttacctcacg tcggtgggca ttccgttcgt gagcggtggc
     3601 cgattcttca ccaacgcgat cgacacccgc acccgcggcg cggacctggt tggaacctgg
     3661 ccagtccagc tgcaaggttc cacgctgaaa ttcaccggcg gcatcaactg gaacaagacc
     3721 gacatccgct cgatcaagcc caatccgccg caactgggcc tggccggcct ggtgctgccg
     3781 atcatcgacc gcgccgaaga gggccgcatc accgtcggca ccccacgcac caaggcgttc
     3841 ttcgcggccg actggggcat cggcaactgg accctgcacg ggcaattgac gaactacggg
     3901 cactgggaat cgctgggcgg aacgccctcc ggcgaccaga cctacggttc gcggacgctg
     3961 ctggatgcct ccatccgcta cgcgctggac cgctgggatt tcagcctggg cggcaacaac
     4021 gtcaccaacg aataccccga gaagaacaac gacgccaaca actaccacgg catcatcgcc
     4081 tacccgctga cctcgccgtt cgggttcaac gggtcctact ggtacgcgag tgcggcgtat
     4141 cggtggtagg tgcaacaggc tggaggacgg acccctctcc tccgggaggg ggcggtcagt
     4201 ccaggcggga gaagcgcacc gcctccccca tgttgcagtt cgtaccgacg agctcgtcga
     4261 cgtgatcatc tgacgccgac aaggtcccgg cagagcattc gtcatcggtt gcaggtgggc
     4321 gtcgacttgc cgcgcgccaa ttcaaacctg cccccgcggc ttcgggcatc cgcgcgcggc
     4381 aattgccggg cgtagcggtt cgccgtgtcc tgcccgccca atcgacgcgc cgcacaggcg
     4441 gcgccagcca ggctgttgaa gcggctgggc gattccgcca acgcgatgcg gaaggcgtcg
     4501 agcgcctcgg cgggccgatc gagttccagt aacagatcgc ccagcaactc gcgcgccggg
     4561 acgagcggcc ccggggtcac cggccgcttc tccgtggcat cctcgagatc ggcggcctgg
     4621 cgcatggcgg ccagtgcttt gcctggcctg ccttgcgcgt gtgcaagcca ggcctgagct
     4681 gccaaccgct gtatttcgac ctggctggcc caatacgcat cgcccgattg ccgcaacgca
     4741 tcctccagcg acgcgagcct tcccacggat cgcgtcgccc gaggcagatc gccgctgcgt
     4801 gccgcgccca atgcgcgcgc gaattccgtg atcgcctgcg cggccgggaa gcgcgcccag
     4861 gggaactccg gatgatgggc gggcaaatcg gcggcttcgc gccactgccg acgctcgatc
     4921 gcgtagcgtg cggggatggc ggcataggcg tatgcgacct tgaaattcgc cggactcgcg
     4981 acgggaatcg cgagcagctc gtcctgtacc gatagcgcat cccggtgtcg tgcgagctgc
     5041 agataggcgt attcgaggta gtccatcgcg tgcaactgct cgtcccacgc tccgggcagg
     5101 cgcatttccg cggcaaagtg cttggccgcg ctttcggctg cgcgattcga agcaaccgat
     5161 tcgttccaca accccagccg cgtgaagatg tgcgagggca tgtgctgcgc atgcgggacc
     5221 gccggtgcaa tgtgcgcata gctgcgagcc gcgcccaatg cgtatttcgc caaggacggc
     5281 gaatcgtagg cgtggatcag gtaatgcagt gcacccggat gatccggttc cacggccagc
     5341 acgcggttca acaggtcagc cgccttgcgt tcccgggcgt gcgatttgtc cggaggtgaa
     5401 gcctgtgcgc tggccagcag ggcaagcgcg tagaacgccg cggcctcggg ttcgcgcggc
     5461 gagccggcgt ggacccgcgc catcgcctgc tcgtaagcca gcgcgcgttc ccgatagtcg
     5521 gtcgaaccgt cgggcccgta aaatgccgcg agcgcatcca gccacgccgt ttcctgttgc
     5581 gtctgctgtt tcgcggcgcg ggcttgcgca agcagcgcac ggccgcgcgc cagcacctgg
     5641 ctatcgggac ggtccggcca cagcggatgc cagaggctga gggcctctcc ccaccacgcc
     5701 atcgcgcatc ggggatcgcg cctggcgatc ccggcaaacg tggcttccgc cttttcatac
     5761 cagaacgaat gcaacagcgt cgtggcttga tcgaacgctt gctgctctcc agggcaggaa
     5821 accgggaagt gcacatggcc gatcacgccc tccggcgcga tcgtcccggg atgttcgtgc
     5881 tgcagcccgg ccgaggctgc cgacgtgccc atcaagcaca acgcaaccag gatgacccgc
     5941 gatatccgga gcatggccgc ctcccggcag ccgcacgcat cggtgccggt cgtagtcgac
     6001 accagaataa tcgttctgcg ccggtgggca agtgccggtc cggtgggtga aaagcgcgca
     6061 ggcggcccct cccggcggga cactcctgat ggggcctcag cgcttgtgga ataccagttt
     6121 gccgccttcg gcgtccaccc ggatcgtgtc gccgccaacg aactcgcccg cgaggatctt
     6181 ctgcgccagc gggttttcga gctgcgcctg gatcgcgcgc ttcagcgggc gcgcgccgta
     6241 cacgggatcg aagccgacgt tgccgagcag catcagggca ccgtcggaaa tctccaggtc
     6301 gatctggcgt tcctgcagcc gcctggcgag gtagttcgtc tgcagccgcg cgatctcgcg
     6361 gatctgcgac ttgtcgagcg gatggaacac cacgatctcg tcgagccggt tgatgaactc
     6421 cggccggaaa tgcgcctgca ccacgcccat cacggcggcc ttcatctggg tgtaggcttc
     6481 cggtgaatcc tcgtcgatgc ccgacgaagc gatctcctgg atctgctgcg atccgaggtt
     6541 cgacgtcatc acgatcacgg tgttgcggaa atccaccgtg cggccctggc cgtcggtgag
     6601 ccgcccgtca tcgagaacct gcaacaggat gttgaacacg tccgggtgcg ccttctcgac
     6661 ttcgtccagc aggatcacgc tgtacgggcg ccggcgcacg gcctccgtga gataaccgcc
     6721 ttcttcgtaa ccgacgtagc ccggtggcgc cccgatcagc cgcgcgaccg agtgcttctc
     6781 catgaattcc gacatgtcga tccgcaccat cgcctcggcg gtgtcgaaca ggaagcccgc
     6841 gagcgccttg cacagctcgg tcttgccgac gccggtgggg ccgaggaaca ggaacgaacc
     6901 ataggggcga ttcggatcgg aaaggcccgc acgtgcgcgg cggatcgcgt ccgaaacggc
     6961 cttcaccgcc tcgtcctggc cgatcacgtt cttgtgcagc gcggattcca tctgcagcag
     7021 cttctcgcgc tcgccctcca gcatcttcga gaccggaatg ccggtccagc gcgatacgac
     7081 ctcggcgatc tcctcggcgg tgaccttgtc cttgaccagc ttgaactcgc gctggtcggt
     7141 gttctgggcc gcggcgagct gcttttccag cgccggcaac tggccgtact ggatctcgct
     7201 catgcgcgcg taatcctggc ggcgctgcgc ggcttcgagc tcgaggtgcg ccttttcgat
     7261 ttcttccttg atcttcgcgt caccctgcag cgtcgccttc tccgacttcc acacttcgtt
     7321 caaatcggag aactcgcgct cgagcctttc gatgtcggtt tcgaggtctt ccaggcgctt
     7381 tttcgattcc aggtcctttt ccttcttcag catctcgcgt tggatcttca actggatcag
     7441 ccggcgctcc aggcgatcga gttcctcggg cttggaatcg atctccatcc ggatccgcga
     7501 ggccgcctcg tccatcaggt cgatggcctt gtcgggcaac tggcggtcgg tgatgtagcg
     7561 attggaaagc gtcgcggccg cgacgatcgc gggatcggtg atctcgacgc cgtggtgtac
     7621 ggcgtagcgc tccttcaaac cacgcaggat cgcaatggtg tcctcgacgt tgggttcgcc
     7681 cacgaacacc ttctggaagc gccgctccag cgccgcgtcc ttttcgatgt acttgcggta
     7741 ttcgtccagc gtggtcgcgc cgatgcaatg cagttctccg cgggccagcg ccggcttcag
     7801 catgttgccg gcgtccatcg cgccctcggc cttgccggcg cccaccatgg tgtgcagttc
     7861 gtcgatgaac aggatgacct ggccttcctg cttggcgagg tcgttcaaca cgcccttcag
     7921 gcgttcctcg aattcgccgc ggaattttgc gcccgctatc agcgcgccca ggtcgagcgc
     7981 gagcagccgc ttgttcttga ggccttccgg cacctcgtcc ttgacgatgc gctgggccag
     8041 gccttcgacg atcgcggtct tgccgacgcc gggctcgccg atcagcaccg ggttgttctt
     8101 ggtgcggcgg ctcaggacct gcaccacgcg gcggatttcc tcgtcgcgcc cgatcacggg
     8161 atcgagcttg ccggtctcgg cacgcgcggt catgtcgatc gtgtacttct ccagcgcctg
     8221 gcgctggtcc tcggcgccct cggactgcac cttctcgccg ccgcggagct tctcgatcgc
     8281 ggcatcgatg ttctgcttgt tcgcacccgc ggcctgcagc gcgcggccga tgtcgccctt
     8341 gtcctcgagg caggccagca ggaacagttc ggacgcgatg aactggtcgc cacgctgctg
     8401 cgccagcttg tcggtgaggt tgagcagccg gttcagctcg ttcgatacgt tgatgttgcc
     8461 ttcctgtccc ctgacactgg gcaggcgctc catgatctcg ccgacgcgct ggcgcagcag
     8521 cggcacgttg acgccggcct gcgccagcat cggcgcggtc gaaccgccct gctggtcgag
     8581 cagcgcggcc agtacgtgcg ccggttccag catgttgtgg tcgcgaccga ccgcgatcga
     8641 ttgcgcgtcg gcgagcgcct gctggaagcg tgaagtgagt ttgtcggtgc gcatggcggt
     8701 gggtgcccag gctgtcgatt cgaccccgaa agatgcggcc gggggctgga agcttcaagc
     8761 gtggccggca ctcacctccc ttcttgcgaa ggcgtcgccg ggcaggggcg aaaggcgcag
     8821 ccacagggcg gcgttgccgg ccgcttgcga tccatccact aaacggtgcc gccctacgct
     8881 cgtcttcccc tctccaccag tttctcgatc aacggtccga gcttgccggc ccgcagcggc
     8941 ttgaccaggt gttcgtcgaa gcccgcctcc atcacgcgca cgtggtcgtt ggcctggccg
     9001 tagccggtga tcgcgatcag cggaaggtgt tcgcggcccg gcgtgccacg cacgctgcgc
     9061 gccacctcgt agccatccat taccggcaac ccgatgtcga tcaacgcgat gtccggctcg
     9121 aactccgcga acgcctgcag cgcggcggcg ccgtcgtaca cgacgcgcgt ccggtagccc
     9181 atctcctgca gcagcagcgc gaggctttcc gccgccacgg cgaaatcgtc cacgatcagg
     9241 accttgcccc ccgcgccatc ggcttcggcc gggggcgtcc gcgggaaccg gacgttcgcg
     9301 accgcggccg cttcgccact cgccggccgc cacggcagca atggcaagcg caccacgaat
     9361 tcgctgccct tgccgtgccc ctcgctcagc gcttccacgc tgccgccgtg catcgcgacc
     9421 aggttgcgca cgatcgccag acccaggccg aggccaccgc cggcgcggtc gatcgcctgc
     9481 cgctcctggg tgaacgactc gaagacccgc gacaacagtt ccggggcgat gccacggccc
     9541 tggtcgcgca cccgcaccac gacctgcccg ccctcggcgc ggacgcgcag gtcgatcgag
     9601 cggcccggca gcgagtattt cgcggcgttg ccgagcaggt tcaccaggac ctgcaccagg
     9661 cggcgccggt cgcccatcac catcgggtcg ccctcgacct gcgtgtccag cgattgccga
     9721 tgctcctcca ttgccggctg cgtggtctcc accgcggcgg cgacgacctc cgccatcgcc
     9781 aagggctcct tctccagttc gaccttgccg cgtgcaatgc gcgccacgtc cagcaggtcg
     9841 tcgaccaggc cggtgaggta gcgcacctgc gcctccatga tctcgcgctc gcgcacgaac
     9901 acatcgggcg cgcgcagctt catcagctgc agggtggtcg cgatcggcgc cagcggattg
     9961 cgcagctcgt ggccgagcat cgcgatgaac tcgtccttga tgcggctggc ctgctgcagc
    10021 ctgtcctggt tctcgcgcaa cgcatcctcg acgcgggcat gttccatcgc cgtccagatg
    10081 cgctccgcag ccgcctcgac cagcgaacga tcttcggcgc tccagtcgcg ggccttggaa
    10141 tcaccgacca ccagcgccca cacataattg cgctcgccct tgcgcaggat cccggtgagc
    10201 acggcgttca tgcccatggc gccgatcgaa cgtttgtcgc gctcggggaa gctggggtcg
    10261 ttgcagaggt cggagatgac gaggggctcc gtctccagcc ggcgcatcga ttccggaaag
    10321 tccctgaagc ggtattgccc ggcgatcggt gggatgttcg gggcgtggta ttccgggccg
    10381 atccacgcga ggtcccgttc gcgcgagaat tcgcagatgt agctgcgctc gacccccatg
    10441 tgttcggcga gcatgcgcgt gcctatggca cccacgttga cgggatcggc ctcggcgcgc
    10501 agagcgtcgc tgagcttcag aaggaatgcc tggcgctcct cgcgtttccg cagcgaggct
    10561 tcggcgcggg cgcgggcttt cgtcgcctgg tgccgggaaa tgatgatggc agcggtgcgg
    10621 gtcacgatcg cggcgaggtg atggtcgccc ggcttcgcct cacgcggtcg ccggtagtac
    10681 atcgcgaagg ttccgaccgc cttgccgccc tccgtttcga tggggaacga ccagcaggcg
    10741 cggaagtcga acgcctcggc cagtgccaac cacggctccc acaacggctc ctcggtcacg
    10801 tccggggtaa tgaccggctg cccgaggtgc gtggccaggc cacaggcgag cgagcctgcg
    10861 ccgatcgcca agccagccat gcgccgtgca tggtcttcgc ccattccgac gacatggtgc
    10921 agcgcggtgc ggctcggatt ggcgaggtag aaagcgcagc ggagttccgg atcccgcgcg
    10981 caagcgacgc gaaccagcac gccgagcgag acggccagcg gttcatcgtt gaccgcggct
    11041 tgcaacgccg ccttttgtcc ggcgagccac gcctcgttgg tgcgcagcgc cgcctcggcg
    11101 cgggcacgct cgaccgccga ccaggtgcgc tcggaaacgt cgcgcaacag cgtgacttcg
    11161 gcatcggtcc aggcgtgcgg ccggttgtcc tgggcagtga cacaggccac gaaacgccct
    11221 tccttgatca gggggatgct gcatgcggtc tggatggaaa acgcttccca agcgctgcgc
    11281 tcggccggcg aaaaccggga gtcggtggcc acgtcggcga tgctcaagtc gcgacccgcg
    11341 ccgagctctt cgaccaggaa ctttccgagg tcttccagcc gatagcgtcc agcggcgacc
    11401 gacggcatgc ctcgccgcgg ccagtcgcgc tcgaagatca ccatgatgtc cggttccgag
    11461 cgcacttcgc agtacgcgac acgcccgata tcgaggtgtt cgctggtaag ccggctggcg
    11521 gcttcctgga tggcgaccgg gtccgccagc ggccgcagcg catcggaaag cctcagcagg
    11581 aaggccttgc gctcttcgct ctggcgcaag gcgatttcgg cgagttcgag ccctgtcgcg
    11641 tcggtgacgt ggtggatgat cgcggcgacg ctgccgtcgg ggctcagcac cggggtgttg
    11701 tacggatccc accagcgctc ctcgaagacg ccatcgggac gcgcgatgtc gtatttctgg
    11761 cgcggcatcg catcggtgcg gccggacgac agcgcacgtt cgagcgacgc gcgcaggttg
    11821 cgcacgccgg tggcggcggg atcgtcggga ttgtcgggga acgcttcgaa caggccgcgc
    11881 cccacgatcc cctcgcgcgt ggtcatggtc gccgcgaggt acgcctcgtt gacgtcgacg
    11941 acggtgaagc gcggcgcgtc cggagccagc agcaggaagg gggtcggggc gttcgcgaac
    12001 agcgcacgat aaagttgccc gccgtcatcg cgggccgcgc tgccgcgcgc gcgttgcgtc
    12061 gccgcgtgcg agtcggaacg ctcggcaaaa cgccgcggag acttgcgctc gatcggcatc
    12121 ctcggcctgt ccctgccacc tcacggctgg tccagcatac accgccgaag gaacgcgccc
    12181 caatgggtgg tcggcaggcc tgtgggcgcc gaacggcgcg catggctcac gccggcttca
    12241 agcgcctttc gccgctcgtc cgctcgcgtg tccgcgcctc gacgagggta tcgatcaact
    12301 gtccgatctt tcccgccgcc aagggcttga ccaggtgttc gtcgaacccc gcctccttgg
    12361 cgcgcgcgtg atcactggcc tggccgaacc cggtgaccgc gaccagcggc aaatcctcgc
    12421 ggcccggggt gcggcgcacc gcgcgcgcca cctcgtagcc gtccatcacc ggcaacccga
    12481 tgtcgaccag cgcgatgtcg ggatcgaatt cagcgaccgc ctgcaggccc gacggcccat
    12541 cctgcgccac ccgggtgaca taacccattt ccccgagcag cagcgccagg ctctccgcgg
    12601 ccaacgcgta gtcgtccacg accagcacct tggccttggt gcggtgcccg gccgggtgcg
    12661 acgcccctgt cacggccttc gatgcaccac gatggcggcg gcgcgccacc agcgggagcc
    12721 gcacggtgaa ttcgctgccc ttcccgcgcc cctcgctcgc ggccacgacg gtcccgccgt
    12781 gcagcgtgac caggttgcgc acgatcgcaa ggcccaggcc caatccaccc gtctggcgat
    12841 ctgccgactg ggcggcctgg gtgaacaact cgaacacgtg cggcagcagg gcgggatcga
    12901 tgccctggcc ttcgtcgcga acccgcaata ccaccttccg gtcctcgcgc gtcgcgcgca
    12961 cctcgatttt ccggccgagc ggcgagtact tcgcggcgtt ggtcagcagg ttcaccacga
    13021 cttgcgccag ccggcgcttg tcgccggaaa ccgccagccc ctccgccacg agcacgcaca
    13081 gcgattgctg gcgctcttcc atcaagggct gggtgatctc caccgccgcg gccacgaccg
    13141 ccgccagatc cacctctgcc gtctgcagtt cgaccttgcc gcgggcgatc cgtgcgacat
    13201 cgagcaggtc gtccaccagg gcggtcaggt gccccacctg cgcgtcgatg atctcgcgct
    13261 cgcgcacgaa cagatccgga ccgcgcagtt tcatcaattc cagggtggtg gcgatcggcg
    13321 ccagcgggtt ccgaagctcg tgccccagca tcgcgatgaa ctcgtccttc gcgcggctgg
    13381 cgcgctgcag ttgcgcttcg ctctcgcgca aggcggcttc cgtgctggcg cgccgggcgg
    13441 cgtcccaggt gcgctctgcg gtttccccga gcaactgaaa ctcgcgcgcg ctccactggt
    13501 ggggcgtggc gaacttggcg atcatcgcgc caacgagcct gccctccttc acggcgggca
    13561 ccgccagata ggcgcggatc ccgagcgcgg caaaggcgcg cttctgttcg ggcgaaagct
    13621 cggccagcgt gtcgagatcg gtgcagccga cgggccggag ttcccggaac atcgccgacg
    13681 cgctgtcctg gaagtcggcg agtcgtaaac gctgcccgac cagcacggtg tctccggggg
    13741 cgccgtcttc cgccgtgacg aggatgcttt cttcctgctg gtcggtttcg cagcagcaga
    13801 cgcggtcggc ggcgagctgc gcccgcacga ttccgcaggc cgcctgctgg atcgcgcggg
    13861 cgtccgtcag cgaccggatc gcatccgaga gcttcagcag gaacgcccgt cgttcctcgg
    13921 cttcccgcag ggcggcacct gcacgcgtgc gctggctgat gtcacgcgcc atcttggagg
    13981 cgcccacggg cttgccatcg ccgtcgttga tcggggaaat ggtgagcgcg acatcgatca
    14041 aggtgccgtc tttcctccgc cgcacggctt cgtagtggtc gatgcgctcg ccacggcgga
    14101 tacgggccag gatcgcctgt tcctcgtcct cccggccggg tggcatcagc atcgtgacgg
    14161 gcttgccgat cgcttcctcg gccgtatagc cgaagatgtg ctccgccccg gcgttccagg
    14221 tctggatgac gccgtcgaga tccttgctga tgatggccac gtcggacgat tcgacgatcg
    14281 cggccagccg gctggtgacg cggtcgttgc gctgctgctg cagacgtgcc tgatgccgtg
    14341 ccgtgacttc gatgaccgtc acgagcacac cctggatgct gccggtttca tcccggatgg
    14401 ggctgtagca cacgtcatac caggcatcgt tgatcgcgcc gttgcgcagg acggggtaca
    14461 gcgcgtcctc gaacatcagc gattcgccct gccatacgcg gtcgtagatc ggtccggcga
    14521 tgtgccagac ctcgggccag cactcccggg ccggctggcc gagccccgcg gggtgcttgt
    14581 cggccatcag gggccggaaa ctgtcgttgt agatctggat gaggtcgcgg ccccagagaa
    14641 ccacggacgg gaagcggttc ggcaggatca attcgaccgc ggtcccgagc gtttgcggcc
    14701 agccgtccat cggtccgagc ggggtcgccg accagtcatg tgcgtggatg agcgcgcgca
    14761 tcccctcgcc gcggcccgca ggccgcgaat catccggcga ttccctttcg tcggccatct
    14821 gatccgccaa tggcaagctc caccctggac cggagtatag cccgcgggcg tggcccggcg
    14881 cccgtggctg ggtggccttg acacgagccg gatggaacgt gaatcgccct ttcacgaggg
    14941 ctccggcaca atatgcggtc gtgcaaccga ctttccgcca ggcaccgacc gcccgcgacg
    15001 ccgacatcgg cccgctgcct gcgccgcccg cgcccggcgg cgacgatgcc tgggccttgt
    15061 tcctcgacgt cgacggcacc ttgctggact tcgcccggca cccggacgaa gtggaagtcg
    15121 ccgagcacct gcacgaggac ctcgatcgcc tgagggtccg gctgcagggc gcggtagcgt
    15181 tgctcagcgg ccgctcgctg gcgcagctcg attcgctctt cgattggcgc gcgcatgccg
    15241 ccggcggtct gcatggtgcc gaattgcgga cgccggacgg cgagcagcat ttcaccggcg
    15301 acgaggactc gttcgcccag gtgcgcgccc gcgcggcgcg gctggtcgac gaggtgcccg
    15361 ggctggtgct ggaagacaag ctccgcgcgc tggccctgca ctacaggcat gcgccgggag
    15421 cgcgccaggc agccgagcgc gtcgcccagg cgttgcttcg cgacaccggc gaccgctacg
    15481 tcgcgcagcg gggcgaccag gtcatcgaac tgaagcccgc cggggtggac aagggccgtg
    15541 cgctcgaggc attgatgcag gtcgtgcctt tccgcggccg ccgtccctgg atgctcggtg
    15601 acgacctcac cgacgaggat gccttccacc gcgtcaacgc gatgggcggc accagcgtca
    15661 tcatcggaga ccgccgtccg acggccgcgc gctgcgcgat cgccagcccg gcggcggtac
    15721 gcgcgtggct gcacgcactg gcgggcgact gacaccgacg gaagcaggac cgccaccatt
    15781 caccccaagg agacttacgt ggccagcaaa acgtcccgat cgaaatcttc ccaacgcaag
    15841 cgcactgccg gcggcacctc ggggaagggg aag
//
LOCUS       DASSNH010000236         6079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1777526, whole genome shotgun sequence.
ACCESSION   DASSNH010000236 DASSNH010000000
VERSION     DASSNH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777526"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFK00_08560"
     CDS             <1..400
                     /locus_tag="VFK00_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7593039.1"
                     /translation="EDTGRHVVHDLERYLNTLGTIALIGPLLGLLGTVFGLIRMFLAV
                     MVSGVGDPMKMAGGIGEALVCTASGLVVAIPAYVLHRYFRSKVQGYVVQMEKQATALL
                     DELSATRPQAVDTRAATASAAPPRPARLAG"
     gene            412..843
                     /locus_tag="VFK00_08565"
     CDS             412..843
                     /locus_tag="VFK00_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7593040.1"
                     /translation="MRIGSGRSDDFEINVVSLIDVLLTLLMFFVLTTTFVQQGRLKVS
                     LPQASDQASAPANDTLVIVIDRQGHFFVGDNRVLGDDEGTLQRAIEAVAGSDRERQVL
                     LRADGQTPHQAVVTAMDALGRLGFAHLSIATAPAKAPAAGA"
     gene            836..2623
                     /gene="msbA"
                     /locus_tag="VFK00_08570"
     CDS             836..2623
                     /gene="msbA"
                     /locus_tag="VFK00_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HET7593041.1"
                     /translation="MPDTPRMQGSDAAVYRRLLGLLRPYRTIVVVTIMGMVVDAAAMT
                     AFARVIKPMIDKLFVTHEPRVIFWMPIVIVAIFFVRSIAVYIESYGSAYVGRGVVQDM
                     RRRIFGKYLRMPAAFFDAESSGQQISRITYTSEQVAQATTDSAKTTIVDGLTVVGLVG
                     VMLWTNWKLSLALLVMVPMIGVLVTYVSRRYRRINVTIQSVIGRVTGAVEEVVGAHRE
                     VKVFGGQDYESARFEEVTDRARKLNIKVAATNGLSTAFVQLAAAIALALIVFFATRPF
                     MLDHLTAGAFATLFLCMGGIMPSLKRLTTVQSNIQRGLAAAAELFGILDAPMERDAGT
                     VELQRCRGDIEYRDVHLTYPGSDAAALRGVSLDCPHGTITALVGRSGSGKSTLASLIP
                     RFYEPSSGTVLMDGRPLGDYTLRSLRAQIAWVGQDVVLFDDTIAHNIAYGALADLDES
                     RIVAAAEAANAMEFIRDLPNGIHSRVGEGGALLSGGQRQRIAVARALLKDAPILVLDE
                     ATSALDTESERLIQDALSRLMVNRTTLVIAHRLSTVEHADQIVVLDAGRIVERGTHAE
                     LLAAGGKYASLHRLQFHEPAAAAPAAATR"
     gene            2991..4007
                     /gene="lpxK"
                     /locus_tag="VFK00_08575"
     CDS             2991..4007
                     /gene="lpxK"
                     /locus_tag="VFK00_08575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET7593042.1"
                     /translation="MKLADSLQLRWYEPSARPAWWTLPLAAFYGALVRLRRRLYRGGA
                     LRSQRLAVPVVVVGNITAGGAGKTPLVIALVEGLRARGFQPGVVSRGYGGTERAPRLL
                     ASGPDPAQVGDEPALIAIRTGAPVAVGANRPAAARLLLDQGVDVIIADDGLQHYALAR
                     DVEICVVDGARRLGNGRLLPAGPLREPASRLREVDFVVCNGGEPRADEIPMRLVADAA
                     VSLADDAMRKPLAAFAGSRVHAVAGIGHPQRFFQSLREMDIEPIEHPFPDHHRYASAD
                     LAFGDELPVLMTDKDAVKCRAFAQPNWWRVPVTAALPESFPDAVAGRLPRPASESTAP
                     QSPS"
     gene            complement(4045..5433)
                     /gene="nhaD"
                     /locus_tag="VFK00_08580"
     CDS             complement(4045..5433)
                     /gene="nhaD"
                     /locus_tag="VFK00_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HET7593043.1"
                     /translation="MSSLSPAIAAATAADADVNFHLTAEPIGWLVLALFALAYVAVIM
                     EERIEIAKSKPVMLAAGLIWGALAWWTNTPGETTSAARAAFEGMFLEYAEIFFFLVVA
                     MTYVTAMGERGVFEALRTQLLKRKFSYRQLFWITGALAFFLSPFIDNLTTALVMSAVV
                     LAVGAGNARFVALAMINLVVAANAGGAWSAFGDITTLMVWQAHKVDFLEFFDIFLPSL
                     VNWLIPAILMFIAVPKGQPGASESHGSIKRGGISICITFGATIAITVIGKQWLQMPAA
                     YGMLTGLALLNLLASRIDYEQRRYAIAQGQEPYERYSIFKIIANAEWDTLLFFYGVFG
                     CVGGLAAAGYLTLASVHLYGGLGFTIANSLMGLLSAVVDNIPIMFAVLKMNPPMDHGQ
                     WLLITLTAGVGGSLLSVGSAAGVALMGASRGMYTFASHLRWTWAIGLGYVASIVVHLL
                     LNAHHFTGAATG"
     gene            5906..>6079
                     /locus_tag="VFK00_08585"
     CDS             5906..>6079
                     /locus_tag="VFK00_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593044.1"
                     /translation="MPSARACNVATKPEDSQRRATRLRAAIEDANHRYYVLDDPSIPD
                     AEYDALLRELEALE"
BASE COUNT         1045 a         1986 c         2061 g          987 t
ORIGIN      
        1 cgaggacacc gggcggcacg tggtgcacga tctcgagcgc tacctcaaca ccctcggcac
       61 gatcgccctg atcgggccgc tcctcggcct gctcggaacc gtgttcggcc tgatccggat
      121 gttcctggcg gtgatggtgt caggcgtcgg cgatccgatg aagatggccg gcggcatcgg
      181 cgaggcgctg gtctgcacgg cctcgggcct cgtggtcgcg atccccgctt acgtgctgca
      241 ccgctacttc cgctcaaaag tgcaaggcta cgtggtgcag atggagaagc aggccaccgc
      301 cctgctggat gagctttcgg ccacgcgccc acaggcggtc gacacgcgcg ccgccacggc
      361 atcggcggcg ccgccacggc ccgcgcggct cgcgggctga cggataccgc gatgcgcatc
      421 ggcagtggtc gcagcgacga tttcgagatc aacgtcgtca gcctcatcga cgtgctgctg
      481 acgctgttga tgtttttcgt gctgaccacg accttcgtgc agcaggggcg cctcaaggta
      541 tcgctgccgc aggcgagcga ccaggcgagc gcgcccgcca acgacacctt ggtgatcgtg
      601 atcgaccggc aaggccattt cttcgtcgga gacaaccgcg tactcggcga cgacgagggc
      661 acgctccagc gcgcgatcga ggcggtcgcc ggcagcgacc gcgagcgcca ggtattgctg
      721 cgggccgatg gccagacgcc gcaccaggcc gtcgtcacgg cgatggacgc gctgggccgg
      781 ctcggcttcg cgcacctgtc catcgcaacc gcgcccgcga aggcgcccgc cgccggtgcc
      841 tgacacgccc cggatgcagg gctccgatgc cgctgtgtac cgacggctgc tgggcctgct
      901 gcgcccgtac cgcacgatcg tggtggtcac gatcatgggc atggtcgtgg acgccgcggc
      961 catgacggcg ttcgcgcgcg tgatcaagcc gatgatcgac aagctgttcg tcacccacga
     1021 gccgcgggtg attttctgga tgccgatcgt catcgtcgcg atcttcttcg tgcgcagcat
     1081 cgcggtctac atcgagagct acgggagcgc ctacgtcggg cgcggcgtgg tccaggacat
     1141 gcgtcgacgg atcttcggca agtacctgcg catgcccgcc gcattcttcg atgcggagtc
     1201 ctccggccag cagatctccc ggatcaccta caccagcgag caggttgcgc aagccaccac
     1261 cgattcggcc aagaccacga tcgtggacgg cctcaccgtg gtcggcctcg tcggggtgat
     1321 gctgtggacc aactggaagt tgtcgctggc cctgctggtg atggtgccga tgatcggtgt
     1381 cctggtgact tacgtcagcc gccgctaccg ccgcatcaac gtgaccatcc agtcggtgat
     1441 cgggcgcgta acgggtgcgg tcgaggaagt cgtcggggca catcgcgagg tcaaggtctt
     1501 cggtggccag gattacgaat ccgcgcgatt cgaagaggtc accgatcgtg cgcgcaagtt
     1561 gaacatcaag gtcgccgcga ccaatgggct ttcgaccgcg ttcgtgcaac tggccgctgc
     1621 catcgcgctc gcgttgatcg tgttcttcgc cactcgtccg ttcatgctcg accacctgac
     1681 agcgggtgcg ttcgcgacgc ttttcctttg catgggcggg atcatgcctt cgttgaagcg
     1741 cctgaccacc gtccagtcga acatccagcg cgggctcgcg gccgcggcgg aattgttcgg
     1801 gatcctggac gcgccgatgg aacgggatgc cggcaccgtg gaattgcaac gctgccgcgg
     1861 cgacatcgag taccgcgacg tgcacctgac gtatccgggc tccgacgcgg ccgcgctgcg
     1921 cggcgtgtcg ctcgactgtc cgcatggaac gatcacggcg ctggtcggcc gctccggcag
     1981 cggcaagagc accctggcga gcctgatccc tcgattctac gagccgagtt cggggaccgt
     2041 gctgatggac ggccggccgc tcggcgacta caccttgcgc agcctgcgcg cgcagatcgc
     2101 ctgggtcggg caggacgtgg tgttgttcga cgacaccatc gcccacaaca tcgcctacgg
     2161 ggcgctggcc gacctcgacg aatcccggat cgttgccgcc gcggaagccg cgaacgcgat
     2221 ggagttcatc cgcgacttgc cgaacggcat ccacagccgg gtgggcgagg gcggcgcgct
     2281 gctctccggc ggacagcgcc agcgcatcgc cgtcgcgcgt gccctgctca aggatgcccc
     2341 gatcctggtg ctggacgagg cgaccagcgc actcgatacc gaatccgaac ggctgatcca
     2401 ggacgcattg tcgcggctga tggtcaaccg caccacgctg gtgatcgcac accggctttc
     2461 cacggtcgag cacgccgacc agatcgtggt gctggatgcc gggcgcatcg tcgaacgcgg
     2521 tacacacgcc gagctgctgg cggcgggcgg caagtacgcc tcgctgcacc gcctgcagtt
     2581 ccacgagccg gccgcggctg caccggccgc agctactcgg tagttgccgt catccccccg
     2641 aatgcgggac tgcgaggcag gacgccggag cgaacatcgg cgaggccgat ggcccgaagg
     2701 gcgcgccgca ggagaggcgc gtaaaccagt gacttcgagt gtattttgag cagcatgtct
     2761 tgatggagca ggcgataacc agggaaaaac aaagaagtgg gaccgccatg gaagcttgat
     2821 cttgtcgaaa aacataaacc ctattggcgc gacttgtatc cggaaccact cggctgagga
     2881 aaggcattgg acaaactcgc catccttggc gctcgccctg cgggccgcct gcggcgttcg
     2941 cgtttgcaat cctggaacgc agtccgcgtg cgcgggaacg acggagttcc atgaaactcg
     3001 ctgattcctt gcaacttcgc tggtacgaac cttccgcgag acccgcatgg tggacgctgc
     3061 cgttggctgc gttttatggt gcgctggtgc ggctgcggcg ccggctgtac cgcggcgggg
     3121 cgttgcggtc gcagcggctg gcagtgccgg tggtcgtggt cgggaatatc accgccgggg
     3181 gagccggcaa gacgccgctg gtgatcgcgc tggtcgaagg gttgcgagcc cgtggcttcc
     3241 aaccgggcgt cgtgagccgc ggttacggcg gcaccgagcg cgccccgagg ctgctcgctt
     3301 cgggacccga tccggcgcag gtcggggacg aacccgcgtt gatcgcgatc cggaccggtg
     3361 cgcccgtcgc ggtcggggcg aatcgcccgg cggcggcgcg cctgctgctg gaccagggtg
     3421 tcgacgtgat cattgctgac gacggcttgc aacactacgc ccttgcgcgc gacgtcgaga
     3481 tctgcgtggt cgatggtgca cgccggctgg gcaatggccg attgctgccc gcgggaccgc
     3541 tgcgcgaacc cgcctcgcga ctgcgtgagg tggacttcgt ggtctgcaat ggaggagagc
     3601 cgcgagcaga cgagatcccg atgcggctcg ttgccgatgc tgcggtatcc ctggctgacg
     3661 acgccatgcg aaaaccgctc gcggcgttcg cgggatcccg ggtgcatgcg gtcgccggca
     3721 tcggccatcc gcaacggttt tttcagtcac tgcgggagat ggacatcgag cccatcgaac
     3781 atccttttcc cgatcatcat cgatatgcgt cggcggacct cgccttcggt gacgagctgc
     3841 ccgtgctgat gaccgacaag gacgcggtga agtgtcgcgc gttcgcccag ccgaactggt
     3901 ggcgcgtgcc ggtaacggcg gcgttgccgg aatcatttcc cgacgctgtg gcggggcgtc
     3961 tgccgcgtcc ggcaagcgaa tccaccgcgc cgcagtcgcc ctcctgacgg cgcgccgtca
     4021 ggggccgatc gctgtcggcg aggttcagcc ggtcgcagcg cccgtgaagt ggtgcgcgtt
     4081 gagcaacagg tgcacgacga tgctggccac gtagccgagg ccgatcgccc aggtccagcg
     4141 caggtgcgag gcaaacgtgt acatgccgcg cgacgcgccc atcagcgcga cgcccgccgc
     4201 cgagccgacc gagagcaggc tgccgccgac gcccgcggtc agtgtgatga gcagccactg
     4261 gccgtgatcc atcggcgggt tcatcttcag caccgcgaac atgattggga tgttgtcgac
     4321 gaccgcggac aacagcccca tcagcgaatt ggcgatggtg aagccgagtc cgccatacaa
     4381 gtgcaccgag gcgagggtca ggtagccggc cgccgccagt ccgcccacgc agccgaatac
     4441 gccgtagaaa aacagcaacg tgtcccattc cgcgttggcg atgatcttga agatcgagta
     4501 gcgctcgtac ggttcctggc cttgcgcgat cgcgtagcgg cgctgctcgt aatcgatgcg
     4561 tgacgcgagc aggttgagga gcgccaagcc tgtcagcatg ccgtaggcgg caggcatctg
     4621 cagccattgc ttgccgatga ccgtgatcgc gatcgtcgcg ccgaaggtga tgcagatgct
     4681 gatgccgccg cgcttgatgg aaccatgcga ctcggaggcg ccgggctgac ccttgggcac
     4741 cgcgatgaac atcaggatcg ccggaatcag ccagttgacc agcgacggca ggaagatgtc
     4801 gaaaaattcc aggaaatcga ccttgtgtgc ctgccacacc atcagcgtcg tgatgtcgcc
     4861 gaacgcgctc cacgcaccgc cggcgttcgc cgccaccacc aggttgatca tggccagcgc
     4921 gacgaagcgc gcgttgccgg cgccgaccgc cagcacaacg gcggacatca ccagggccgt
     4981 ggtcaagttg tcgatgaaag gcgacaggaa gaatgccagc gcgccggtga tccagaacaa
     5041 ctgccggtag ctgaacttgc gtttcaggag ctgcgtccgc agcgcctcga acacgccgcg
     5101 ctcgcccatc gcggtgacgt acgtcatcgc caccaccagg aagaagaaga tctccgcgta
     5161 ttccaggaac attccttcga atgcggcgcg cgcggctgag gtggtttcgc ccggggtgtt
     5221 ggtccaccac gcgagtgcgc cccagatcaa gccggcggcc agcatcaccg gcttggactt
     5281 ggcgatctcg atgcgctctt ccatgatcac cgcgacatag gccaacgcga acaacgccag
     5341 caccagccag ccgatgggct ccgcggtgag gtggaagttc acgtcggcat cggcggctgt
     5401 cgcggccgcg atcgcgggcg acaggctgga catggcgagc atcgggaccc ctcggacgac
     5461 ggcgtgtgcg cagccaagcc taacagccgg cttgacgtgg gcgcgagact gacctccggc
     5521 gggacccggg ggtgcggcgc tttgcgcaac gccacacgaa gcgcaaaccg cgcctggcgt
     5581 cagtcgacgc ggctgtcgcg aaaagtgggg ccaaggattg ccgttccgct ttggcgcgga
     5641 cgggaagtgc gctagacaaa ggcgtctccg gcctgcgcca ccggggaggg gtaagacgca
     5701 ggccggatac gcggatgagg ggagccaacc aggcagtggc acgagggatg cccgagcctg
     5761 atcgctgcaa aataggggcc gcacctcacc cgcgcaacat ggtgctagcg caacctttcg
     5821 ccgacctttc gcgccggaaa atggccgcgg ccaaggcttt gcggcgggtt ccctgccgcg
     5881 cccgactata cttgcagcgg tcaggatgcc atccgcacga gcctgcaacg tggccacgaa
     5941 accggaagac agccagcgac gggccacgag gctgcgcgcg gcgatcgaag acgccaacca
     6001 ccgctactac gtcctcgacg acccatccat cccggatgcc gagtacgacg cgctgctgcg
     6061 cgagctggaa gcgctggag
//
LOCUS       DASSNH010000237        32624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_602338, whole genome shotgun sequence.
ACCESSION   DASSNH010000237 DASSNH010000000
VERSION     DASSNH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 32624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32624
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602338"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFK00_08590"
     CDS             complement(<1..380)
                     /locus_tag="VFK00_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7593045.1"
                     /translation="MRFHLAAVLLSGLIVQGAHALPYSPEPTSAGDRPSRAVSHHPFR
                     MPESERKNIAENPLWSAPQKPFRIYGNTWYVGPRGLGVFLVTAPTGDVLIDGGVPGEA
                     ALIEANIRSLGIDLRDIKWILNSHA"
     gene            complement(522..884)
                     /locus_tag="VFK00_08595"
     CDS             complement(522..884)
                     /locus_tag="VFK00_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593046.1"
                     /translation="MNPQTMLLTCFILFAITAVLGLSMAAIREARKHNPPAWLTMFHG
                     LPAAAGLTLLLYATFAFGVPRLAVIALILFLVAAGGGAMLNLAYQQRGQLLPRWLMYL
                     HLLLAVCGFVLLAIVVFA"
     gene            1218..1793
                     /locus_tag="VFK00_08600"
     CDS             1218..1793
                     /locus_tag="VFK00_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7593047.1"
                     /translation="MNDAVLGATFFAMDPGDFDHETALVARAQAHEVKAFEALYRLHV
                     GRIHALCRRLAGSHALAEDCTQEAFVQAWKALPGFRRDSAFGSWLHRIAVNVVIGHQR
                     TALRRSAWGREDGDDALDSCAAPAERPDVALDLDRAIAELPPGARTVFVLYDIDGYKH
                     EEIAELTGLAVGTCKAHLHRARRLLRARLTA"
     gene            1790..2338
                     /locus_tag="VFK00_08605"
     CDS             1790..2338
                     /locus_tag="VFK00_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593048.1"
                     /translation="MNNETGTDEIRDERLRKAVGSLPREIPPEHDLWPGIAARIAPAR
                     HRFQTWSIAAGIACMVAAGAIVTTLSQHRQQAPATLATRQPVTQPRPAAATPLPNERA
                     EMLAATIRRSTHEDPHTQAVLLRNLDLVETSITHLQQALDEDPANPGLQPLLYQQYRN
                     EATLVAAAQRVQLQTATGAIAL"
     gene            2335..3261
                     /locus_tag="VFK00_08610"
     CDS             2335..3261
                     /locus_tag="VFK00_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7593049.1"
                     /translation="MNNHTHPNVKRFALPAGLLFALLAATLPAFAGTPVDKTVTATPN
                     GHVQVSSVAGSIKVTAWNRNEVHVTGSLSRNAERLAVEKTGTGVNIQVVLPQHSSGNM
                     EGSDLVIEVPAASQLEVHTVSADIDAGGLGGPVRLESVSGNVGLVSSSANIYANTISG
                     DVRVTGSAPGATIEGHSISGKVLIEKVNGSLRAGSTSGDIELTGENSLQAAKLDTTSG
                     DVHFAAALGADGDYEFHSVSGNVVLSLPKVPAGSFDVSSFSGDIDTDFGPQPKRSSEF
                     GPGKEWHYQSGSGQADIRIRTLSGDVRISAAH"
     gene            3383..4807
                     /locus_tag="VFK00_08615"
     CDS             3383..4807
                     /locus_tag="VFK00_08615"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7593050.1"
                     /translation="MSHASIASNPILNTDSYKASHWLQYPPGVDATFFYLESRGGTYE
                     RTLFFGLQSILKEYLSKPVTPAMVDEAKAFFPAHGEPFNETGWRHVVDAHGGKLPLRI
                     RAVPEGTVVPTHQALMTIESTCPDCFWVPSYVETLLMRVWYPVTVATISWHVKQTIRE
                     FLDKTSDDPAAQIAFKLHDFGARGVSSAESAALGGMAHLVNFRGTDTILGVLAAREYY
                     GEEMAGFSIPAAEHSTITAWGKDRELDAYRNMLRQFAKPGSIVACVSDSYDVFRAVKE
                     MWGGALRDEVVQSGATLVVRPDSGDPADIVHSTLKMLNAAFGSTVNAKGFRVLDHVRV
                     IQGDGVNPDSIRAILERITRAKYSADNLTFGMGGALLQKMDRDTQRFALKCSAARIDG
                     QWMDVFKDPVTDHAKASRPGRLTLARHREFGTFKTVPLPDGAIEADAATLGAGWEEAM
                     TTVWEDGALLRDWTFTEIRARGET"
     gene            4921..5181
                     /locus_tag="VFK00_08620"
     CDS             4921..5181
                     /locus_tag="VFK00_08620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7593051.1"
                     /translation="MHDADERLFHADVDGHRGELDYVLRDGVMTITHTGVPPAIGGRG
                     VAAELTRVALETARRKGWKVVPACSYAAAFIRRHREFADLLA"
     gene            5267..6172
                     /locus_tag="VFK00_08625"
     CDS             5267..6172
                     /locus_tag="VFK00_08625"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7593052.1"
                     /translation="MPAKPAPDKPASDQPAAGKPAATRPAAGKPPEKLHFISGLPRSG
                     STLLSAILRQNPRFHAGMTSPLGDMISVLVAEMSGKNDFSVVINDAQRTSVLRGVFQN
                     FYGGLHGTDVVFDTGRVWCSKLPMLATLFPNSKVIACVREMPWVLDSVERLVRKQPLS
                     ANKIFHFNPNGTAYSRAEALIDVNGMVGFSFQATKDAFYGHFAPGRLMLLRYESLTQD
                     PGSAMQALYKFIGEPWFEHDFDHIEYAADEFDSRLGLPGLHTVKSRVSAPKRESVLPP
                     DLFNRFVKDSFWLDPKNNIRKVLVV"
     gene            complement(6313..7482)
                     /locus_tag="VFK00_08630"
     CDS             complement(6313..7482)
                     /locus_tag="VFK00_08630"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HET7593053.1"
                     /translation="MQDSYAAGDPGAAATIAIAGLAALAVAMGIGRFAFTPVMPMMEA
                     DAGLALRTAGWLAAANYLGYLLGALCAARLSFGQAARGGLLLIAVATFAMGLTHALAL
                     QLVLRLVAGIASAWVLVHVSAWALNGLLSLRRADAAGILYAGVGIGVAIAGLIVMAAM
                     QLGMDSDRTWQVLGGLSLLATLALWRVLRGPWAAPAPPAVEDARPRIRWTADERRLAW
                     CYGALGIGYIIPATYLPEMAHRFVTDPLVFGLAWPVFGATSALSTWIAARWLARIADR
                     KLWATCYVMMAAGCALPALWPTFAAILVSALLVGGTYMVVTMLGLREARRVAGAGATA
                     FIAVMTAAFAIGQIIGPLLVSAVAHLPHGFSATLLAAAVVVLLAAASLWRSARLA"
     gene            complement(7549..8025)
                     /locus_tag="VFK00_08635"
     CDS             complement(7549..8025)
                     /locus_tag="VFK00_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7593054.1"
                     /translation="MATNRLLKSLRTLHLYFGVFTAPALLFFAFTGGLQSLSLHEASR
                     VGDYQPPRWLASISHLHKKQSLDVPVKRRSRNASVSKQGAEADVMSARAADKPLPAPR
                     KRNPWPMKVFFVLVSLSLLLSTVSGVYMGWRCARNRRRYGAALFAGVAVPALLLLF"
     gene            8180..11518
                     /gene="recC"
                     /locus_tag="VFK00_08640"
     CDS             8180..11518
                     /gene="recC"
                     /locus_tag="VFK00_08640"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HET7593055.1"
                     /translation="MFRLVTSNNAGTLADELGDGLRDPRGNPLTPALVLVPQAGLRRW
                     LQVHLAERLGVIANVEFRAPAEFAWDLLRAAKPDLPRRSPFDPDVMRWHLHALLGESL
                     EGAAMAPVREYLQSHGDPLRRYALAGELAHVFERMQGYRRAKLLRWERHEESPDSDPQ
                     AWQAELWRRLIARTGGTSRAGRMEGWLRSFDPDYQPYAAAGKPAPPGLPSRLTCFACA
                     NVSPDVLRMLAVAGLHCDIDFYLPLPSREYLGDLPRSRQEVRERLAEGSGENPLVISM
                     ATASREFLELLYDYGCVQPDSESERFDESIARDTLLGRVRDDILAHRAAREDDRVALP
                     DDSLQFHACHTALREVQSLHDRLLAMFAADPTLKPREIAVMMPDVAAYRPAIEAVFGS
                     LPDHDPRFIPYNLGDLAAAATHPAARLFLSLMDAPTSRWRLSEILDVLAVPGVMRRLD
                     LDAPELEQLSRQLKESGARWGEDEHARAQVGGYREFSFAFGLDRMLAGFACGDDDEAL
                     VAGVAPLAGVEGAAFARLDALLAVLDAWKRLREFAARRMDAAEWQRALNGLFDALYAG
                     DPGDIAEARALERVRGALEDLVEHTRAAHLQQPLAWHDLRAFLRERMEDADPRQQLFS
                     GGVTFCGMVPLRVVPFRVICLLGMDEAAFPRRDPSGLDPLSADRRSGRAQRGDRSVRE
                     DDRLLFLQLIAAARDALYISWIGRDAHSNETLAPSVVVAELMDVLREGYLAQDDDSRA
                     SSAPLLPRVQPLHPFDVSLFDGRAPRSYGAEWLAAARRPDARAADAPFMPDAPLPRLE
                     AAQAAALTLEDLKRFLLDPARGFLEQGLGLALPRGRPADADEEPLSPRDGLTRWSLTR
                     ALLDFGEGNASGDRDLLRARGQLPPGALGDEALRDAHVQADALRAVVLEFTRGAPPSP
                     AHPLRVDWDDGTRLEGAPADPYPGGVLHVRPGEIDGRHVLRAWLDALLCSAGGTGKPV
                     MLVGLGEQDACSFALPQLAREAARGELRALIELCREGRCAPLPFFVRVSWKHALACAK
                     AEKKSGTPPGRADIAVFEKAAKQAEGDNGFGGSDEFGQDAVCMAWRGRELPGPADGGL
                     AQDLHRLALAVFARPARAWAEQFP"
     gene            11515..15138
                     /locus_tag="VFK00_08645"
     CDS             11515..15138
                     /locus_tag="VFK00_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7593056.1"
                     /translation="MTPLDLPLSGVRLVEASAGTGKTFTIATLYLRLVLERGLVPGSI
                     VVATFTRAATAELAMRLRARLRLASEILRDADPARERGGDDGETQATRAVIAQALGAA
                     DAGTLAGRARDAELAMDTAMIGTLHAFCNRVLNEFGFETGRAPGELELLEDPRALQQE
                     IMEDFWRSCSSDADAARLLAETWRTPEALAKQVCEPRWRARRVVAGDAVQIPAQQRRV
                     QREATLAQLDAIRNAIAGWDDAALQVAMQELADCISHQGARKARAQGLQALRDWARAG
                     TAADLLSAAARKAVDGLAADKLSEMKTCTRLPEGPVFAAIADLDANIKSLEQLDQAAR
                     ARLLLDARGWLERELPERMKALGKLGHDQVVDALAAALQDPQRGAQAVRAIRERWPVA
                     LVDESQDTDPQQWRILEKLFAHENGALVLVGDPKQAIYGFRGGDVHAWLMAKREAQGE
                     PLRLDESQRAGAGVNAAINALFSGDNSFVEPGIVHEDVHAAPRVAERALLVDGEPAPG
                     LTVWRVPSTGITNKDGGTRAWYKGDAQARIETACVAQIVDWLEGARRGRVRLREGSGD
                     SRELCPRDIAILVNSNREAKSMQRALGAAGVPAASCLRASIYASGEAADLRLLLQALR
                     DPGDAARARAARASLLVGDDALAIARTCSDGAALDALLTETAEWAALVQRRGPLPLLH
                     RLVARAAPRLLAAPGGERRVANYLQLADLLQDEYATCFGIDDLCEHYARAIRESGNDT
                     DADAVRLRLDTDAQAVQIATVHAAKGLEYSVVLLPCAVSGHGNGGSGRKPALTWHHEG
                     DRACVAVGSGVAAEIETLAHREALAEEVRKFYVAVTRARAACFMPWGCVRQADETAAH
                     WLLHVARREAPLGCDEPACEQALSELVARGSGHVAVSPLPEARSKRLSKARSGTTRIE
                     AARFHGDVERDWRAWSFSRLVRGEARGAAADPAPGHGDSPSAAGDARPQAAPMLAGAR
                     FGSAVHAALERADFDAWRGTDGIPELQRDLIARSLRAQGLPGPGTISMERAVTLVGEY
                     MRAALNVALACGTRLCHIQPERRCAEMEFHLRLAPSRVDALFALLHEHGYQRGRSGFG
                     VGRLHGLLTGVMDLVFEHNGRYHLVDYKTNLLPAYDAGALCEAIATHDYDLQYLLYVL
                     ALHRWLRQAVPGYDYDAHVGDVYYLFVRELTDGRGVHRDRPPRTLIEAMDALFDGEVE
                     QAA"
     gene            15135..16832
                     /gene="recD"
                     /locus_tag="VFK00_08650"
     CDS             15135..16832
                     /gene="recD"
                     /locus_tag="VFK00_08650"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HET7593057.1"
                     /translation="MSADLQALARAGWLRRVDAALGEWVAGAFPDAPAEVALAAALAA
                     RAVGEGHSALQLDAAQAWLGHLDGRGDGPALPDPAAWQATLRTSEAVHCGSSLDANDP
                     VPPRPLVLDAEGRVYLARYFDYERRLARNLVARAHTERLKLTTGGPGTGKTHGVVRLL
                     ATLAGKAHAQARGLRMALAAPTGKAAARLSQSVRAQLPRLGLPGTVDAMIPREASTLH
                     RLLGLSPANARAKFHRGAPLPYDVVVVDEVSMVDLPMMYRLADAVRDDATLVLLGDPG
                     QLAAVEAGDVLAALVTAAREPPLSRCHTHLTQSRRFAGHGALARLANAIAGGDAQAAL
                     ATLQEANDEVRLLADGTRSARLVEDAADSYRTVLDAADAGAALRAANGFRVLTALRHG
                     PAGNLALDHAIEQRLKRRAGVRADAPWWPGRLLMVTANRDGLGLFNGDVGVVWPDANG
                     EMKVWFEGTEGAPRAVSTAALPPHEGAFALSVHKAQGSEFGRVALVLGADTPVLTREL
                     LYTGITRAREGVEIHGDEALLRAGIARRTQRWNGLADRLREAANAPISIAESLPGPT"
     gene            16939..21432
                     /locus_tag="VFK00_08655"
     CDS             16939..21432
                     /locus_tag="VFK00_08655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7593058.1"
                     /translation="MHAFHPAVSAWFESAFDAPTEAQARAWPAIQSHQDVLIAAPTGS
                     GKTLAAFLAAIDQLVRRGLEGTLNDETHVVYVSPLKALSNDVRINLEVPIEGIRAELE
                     RTGLPDVEIRTAVRTGDTPQAERTAMRKRPPHIVVTTPESLYILLGSESGRAMLATTR
                     AVIVDEIHALTPNKRGSHLSLSLARLDALCGRRPVRIGLSATQKPIEEVARFLCADRD
                     CTIIDAGHQRARDLGIEVTNVPLQPVMSNDAWGLVYDRIAELISQHRTTLIFANTRRT
                     VERATRHLSERLGKEAVAAHHGSLSKEKRLDAERRLKSGQLRALVATASLELGIDIGE
                     VELVCQLGSPRSIAAFLQRAGRAGHAVSGTSKARLFPTSRDDLAECVALLDCVRRGEL
                     DTLHVPRAPLDVLAQQIVAEVACGERDENELFDLVRSAYPYRDLARDKFSEVLRMLAE
                     GYSTRRGTRAAYLHRDAVNGKLRARRNARLTAITSGGAIPDTADYDVVLEPAAQRVGS
                     VNEDFAVESIAGDIFQLGNQSYRILKVERGRVRVEDAHGAPPSIPFWLGEAPGRSDEL
                     SWAVSRLREEISARLSESPLPQTAASFGERRPQAERVALVRGQDVAESGGSAADRSLT
                     PLTPALSPNESVGGEGAQAALVWLRDEVGVVEPAAKQLLEYYQAARTELGVLPTHAML
                     AMERFFDESGGTQLVIHSPYGSRLNRAWGLALRKRFCRTFNFELQAAATEDAIVLSLS
                     TSHSFPLEEVSRYLHSNSVRGVLTQALLDAPMFALRWRWNASSALALPRFVGGAKVPP
                     QIQRMKSEDLLATVFPDQVACLENIVGERKIPDHPLVEQTLQDCLYDAMDLEGLVALL
                     RKLEAGEVTIVARDLTAPSSLCAEVLGARPYAYLDDAPLEERRTQAVVSRGFTDAGRA
                     EDLGKLDADAIAAVREEAWPQPRDADEMHEALLALGAITDGEAAHDVGWNDHLEELVR
                     ANRATRMLLKLPLARKGEGERACLWVAAEMLPMWRAIHPDAAMQPAIEAPAEFAARNW
                     SREDALLDIVRGRLGCVGPTRVGELAQTFGASDSDIAIALAALEREGGAMRGRFTPGA
                     YDEEWCDRHLLARIHRRTLQRLRREIEPVAPRDFMRFLADWQHVSPGARLRGPDALAG
                     VLAQLEGFEAAAGAWESQLLPARVAGYDIAWLDALCGSGRIAWTRLRAASGGAHASPV
                     RASPIVLLPRKQLATWNAIGAQSAGEAPALSSRAQAVVEFLSRHGASFFGEIADGTRL
                     LKVELEDALGELVGAGMVGADSFAGLRALLLPTAKRRGARQRRLARHMLNGIEDAGRW
                     SLIRRGDPAPPAHPGEGEHIEHIARTLLRRWGVLFWKLLQREAAWLPPWRDLRRVYQR
                     LEARGEIRGGRFVEGMVGEQFALPEAVEVLRRVRKREHDGALVAVSGADPLNLVGDVV
                     AGPRVPAVAASRIAYRDGLPVAIRVAGEVTLLEALSPEEERALRARMRAVVPDRRLVQ
                     PLA"
     gene            complement(21507..23366)
                     /locus_tag="VFK00_08660"
     CDS             complement(21507..23366)
                     /locus_tag="VFK00_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HET7593059.1"
                     /translation="MADAADARIPPPQDTPYAGTIKIVVDASDTSQGIFRVHETIPVQ
                     AGELTLLYPKWIPGNHSPTGPIDKVAGLTITAGGKPLAWTRDKYDVHAFHVDVPHGVS
                     SIDVEFQFLSARSHAEGPIEMTGKMLDLVWSKVSLYPAGHYTRDIAFAPSVTLPAGWQ
                     FGTALKVAHQDRQSQSGNTTTFEPTTYNTLVDSPMYAGKYFKRVDLDPGAKVPVHMDI
                     VADAPKDLEITPEQLQLHRNLVAQAYKLFHSHHYDHYDFLFSLSDELSGKGLEHHQSS
                     EDGARPEYFTDWKNNVAGRDLLAHEYTHSWNGKFRRPADLWTPNFNVPMGDSLLWVYE
                     GQTQYWGYVLTARSGLWTPDQFKEALALVAARYDRGRTGFAWRTLQDTTNDPTIAQRA
                     PLPYRNWQMSEEYYSAGQLLWLAVDAKIRSLTHDSKSLDTFAKAFFGVDNGSFLTSTY
                     TFDDLVAALDGVAKYDWANFLRSRLDAHQPPLDGIEAAGWKLVYNGEESDFQKQIAKR
                     YHAQRSFLFSIGLAIGKDGDINDVRWNGPAFKAGVGSGEKLVAVNGQAYKSGVLADAI
                     KAAQDDKSPIELLLEYQGHYKTVPVDYHGGPQYPHLVRIEGAPDYLSQIIKAK"
     gene            complement(23474..25351)
                     /locus_tag="VFK00_08665"
     CDS             complement(23474..25351)
                     /locus_tag="VFK00_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HET7593060.1"
                     /translation="MGFGGAAFADAADAHIPPPQDTPYPGTLQVHVDASDTRQGIFRV
                     HETIPVEPGELTLLYPKWLPGAHSPSGPIDMLAGLKVAAGGEPLRWKRDKYDVYAFRV
                     DVPQGVSSIDVDFQFLSGRGKFGFIQMTDKMLSLEWNAVSLYPAGHFSRQIRVLPSVT
                     FPAGWQFGTALEEQSRSGDTVTFEPTTYNTLVDSPIYAGKYFKRLDLDPGAKVPVHLD
                     VFADAPKDLEVTPGQLQVHRNLVSQAYKLFGSRHYDHYDFLFSLSDQLSGNGLEHHQS
                     SEDGMGPDYFTNWDDNAPNRDLLAHEYTHSWNGKFRRPADLWTPSFNVPMGDSLLWVY
                     EGQTQYWGFVLTARAGMWTPEQFRDALAMVAANYDRNRPGFQWRTLEDTTNDPTAAHR
                     AMLPYRSWQMSEEYYSGGQMMWLAVDAKIRSLTRGRKSLDDFAKAFFGVDNGSFVTNT
                     YTFDDVVAALDGVVKFDWKGFLDRYVDELEPPFTKGLEATGWKLVYTDEESPYEKQYE
                     SQDESPRHMYNFTYSIGLTLTKDGGINDVRWGGPAFEAGIGSGGEVVAVNGQDYKAAV
                     LKQAITAARDSKQPIELLIKYQGEYATVPVDYHGGLQYPHLVRIKGTPDYLSQIIKAR
                     K"
     gene            complement(25502..26203)
                     /locus_tag="VFK00_08670"
     CDS             complement(25502..26203)
                     /locus_tag="VFK00_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET7593061.1"
                     /translation="MIDLYYWPTPNGHKITLFLEEAGVDYAIKPVDIGKGAQFEPGYL
                     AISPNNKMPAIVDHDPAGGGAPISVFESGAILMYLADKAGKFIPADARGRVECLEWLF
                     WQVGGLGPMLGQYGHFHVYAPEKLPYAMERYRKEAERLLDVLDRRLAGRAFITGDDYS
                     IADMACYPWIDPYSKAPLDLEPFADVRRWRDAIAARPATARAYARATEANPSAGQPMS
                     DDEKKILFGQGARSR"
     gene            complement(26315..27796)
                     /locus_tag="VFK00_08675"
     CDS             complement(26315..27796)
                     /locus_tag="VFK00_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7593062.1"
                     /translation="MNLFQKMIRHKSVEQLQADAGGSQDFRRTMGVWQLTAIGVGAIV
                     GVGVFVLAPHEAALHAGPAIVISFLIAGIGSACAALSYAEFAGMIPVTGSAYTYGYSV
                     LGELPAWIIGWDLLIEYALIVAVVAAGTSNYLQSLIQQITGWQLPVYLQGAYGEGDGM
                     MFNLWAAVVALLIAGLQIFRTEIGARFNTVVVALKVVGVVIVIVVGAFYVNPDLWHPF
                     VPAVVEHADGTSQYGWAGVMAAASIVFFACFGYDTLTTAAEESKNPQRDLPWAVVLSL
                     VVAMAMYFGVSLVLTGMVHYTDLNVPAPVSAAFQERGLHWIGGIINVAAVCGIASVVF
                     AFMLGAARIWFALSRDGLLPRWFAKSHPKYGTPYRTTAILGVATAVTAGFVPLTELAK
                     LVNIGTLSAFILICTSVLVLRIRRPDLERRFRTPALWLLAPLGVVFSLFLIIGWPWVT
                     NGEFHLIGGLDRDTYIRFLVWMLIGFVIYFGYGIRHSALNRVE"
     gene            27977..29032
                     /gene="ubiA"
                     /locus_tag="VFK00_08680"
     CDS             27977..29032
                     /gene="ubiA"
                     /locus_tag="VFK00_08680"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HET7593063.1"
                     /translation="MPRKPARTRRESRPAHPVTQQELPLLDLRAPELQPAGAPDTSRP
                     ADASPRSTRTIEFLLRALPIGWRDKARDLLVLVRMDRPIGALLLLWPTWWALWLAADG
                     FPPWKFLVVFTVGVFVMRAAGCAMNDFADRKLDPRVQRTAGRPIAAGRVKPREAVMVF
                     VGLCLLGFALVLAFTNTLTIYLSFAGAALAALYPFSKRYTHLPQVVLGAAFGWGIPMA
                     FAAITNGLPPVCWLLFFGNVLFSTIYDTEYAMVDREDDLRAGAKSTAILFGDADLPIL
                     GILMATLLFTLLLVGQRAQLEWPFLAGLGVALLLFAWQLWSMRWRERDACFAAFRNNN
                     WVGGMVWLGMVVALAMK"
     gene            complement(29242..29994)
                     /locus_tag="VFK00_08685"
     CDS             complement(29242..29994)
                     /locus_tag="VFK00_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HET7593064.1"
                     /translation="MTGEWLELAGRGGTIKVLKAMHPRARRLRLSVTPHGARVSYPRG
                     THPAQIYSFLRENSDWLERKLSEMRVPRRGPPPLRAGVPTLIPLRGESVELTWRESEY
                     PRIEDRGDELRLHVPRPFTHAMPAARNLLATFLETRMRRDLSRWMAHAVERLDVAPTG
                     MRIKPLKSLWGSLDARDRITLDLALALAPPAALRYVLVHEMCHLKVRSHAPRFWARVE
                     SLMPGYQAQRDWLREHGAILKAEMERLIAPSP"
     gene            complement(30024..30941)
                     /locus_tag="VFK00_08690"
     CDS             complement(30024..30941)
                     /locus_tag="VFK00_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HET7593065.1"
                     /translation="MFFRNLTLFRFSASAAKSLKDLDAALADHVLRACGPLEVATRGF
                     VSPFGPDSEVLSHAVGKATLVTLGTEEKLLPAAVVNAELGARLRKEAARRGRPVGGRQ
                     RRAIKAEVLDTLLPQAFVRPSRLNVYLEAKSGWLVLDTASRKAAEGALTALREALGSF
                     PAQPLAAAETPRALMTDWLASGKLPEGLAFGDECELRDPAGGAVIRCRRQELESTEVR
                     AHLKQGKQVFQLGLQFEDRASFTLGEDLVIRKLRFLDVVQDSLDADGRDSAEAELDAR
                     FALMTLELERLFAGLEKWFGLQRPDAKDA"
     gene            complement(31022..31900)
                     /gene="ttcA"
                     /locus_tag="VFK00_08695"
     CDS             complement(31022..31900)
                     /gene="ttcA"
                     /locus_tag="VFK00_08695"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET7593066.1"
                     /translation="MSATHVQSVPGRKQAHEAARLAKRLRHAVGRAIADYNLVEDGDR
                     VMVCLSGGKDSWTLLDLLLSLKAKAPVRFELAAVNLDQKQPGFPGHVIPDYCRERGVP
                     FHVIEQDTYSVVTRVVPEGRTMCSLCSRLRRGALYAFAEREGFTKIALGHHRDDILAT
                     FFLNLFHGGTLKAMPPKLLSDDGRHLVIRPLAYCRESEIAEYATERAFPIIPCNLCGS
                     QETLQRRQVRKMLDGWEREHPGRSETMFRALRHVAPSQLADRELFDLAGLGARGETAR
                     PDTHAWLSGDAGAGDG"
     gene            31947..32156
                     /locus_tag="VFK00_08700"
     CDS             31947..32156
                     /locus_tag="VFK00_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET7593067.1"
                     /translation="MQPGNDPATLIRRLAELREEHRDLDAAIAKLVGDPSCDQLQLSR
                     LKKRKLKLKDMIAYFEDKLIPDLDA"
     gene            32228..>32624
                     /locus_tag="VFK00_08705"
     CDS             32228..>32624
                     /locus_tag="VFK00_08705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7593068.1"
                     /translation="MTELIPTVLQDGHVALEPLSLDHVAGLEAAAADGELWNLRVTYV
                     PPPDAMQAYVETALAMQAAGDSLPFAVRDKRDGALVGSTRYYGFDAETPRLLIGHTWY
                     ARSRQRSPVNTACKLLLIGYAFERLGCAAV"
BASE COUNT         5376 a        10914 c        11203 g         5131 t
ORIGIN      
        1 gcgtgcgaat tgaggatcca tttgatgtcg cgcaggtcga tgccgagact tcggatgttc
       61 gcttcgatca gcgcagcctc cccgggcacg ccgccgtcga tcagaacatc gccggtcggt
      121 gcggtgacga ggaagacgcc gaggccacgg ggtccgacgt accaggtgtt gccgtaaatg
      181 cgaaacggct tctgcggggc tgaccacaag ggattttccg cgatgttctt ccgctccgat
      241 tcgggcatgc gaaaaggatg atgtgacaca gcccgcgatg gacgatcacc cgcgcttgtg
      301 ggctccggtg agtatggcaa cgcatgcgcg ccctgcacga tcaaaccgga aaggagaact
      361 gcggccagat ggaagcgcat ggtcgaacgc cttgggaaag cgtgcaggat aatgcgccgt
      421 cgcgctcggc gatcgcagta cgcgacttac cgccccgaac ccgtcaacgc ggctcgccct
      481 gcgccgcggt gacaccggca gcgcaatctt tcgcacgcac ttcaggcgaa cacgacgatc
      541 gccagcagca cgaagccgca cacggctagc agcaggtgca ggtacatcag ccagcggggc
      601 agcaactggc cgcgctgctg gtatgcaagg ttgagcatgg caccgccgcc tgcggcgacc
      661 aggaacagga tcagcgcgat gactgccagc cgtgggacgc cgaatgcgaa cgtggcgtac
      721 aggagcaagg tcaggccggc ggccgcgggc aggccgtgga acatcgtcag ccaggcgggc
      781 ggattgtgct tcctcgcttc gcggatcgcg gccatcgaga ggccgagcac ggccgtgatc
      841 gcgaacagga tgaagcaggt caacagcatg gtctgcggat tcatggcggt gcctccttgg
      901 aatcggcaat cacccgtggg gggcaggaaa caagggacca gcaacgatga aacgcgtctc
      961 gctcgcgggg cgttttgcgg cccggcaaaa aaagcagtgg cagtcggccg gggtcacgct
     1021 agcgctgccg ctccaggcgg tcaacggtgg gtggaaaaag ccggtggccc ttcgtcggca
     1081 cgcctgatcc ggattgatgc aaggcagcga agcaaggctt ggatgggcct gtcctacgct
     1141 atcgccgtgg caatgcctgc agcttgccct actccaggcg agtaaacctt tccgccccac
     1201 cccgcgtctt atgccacatg aacgatgccg tgctcggcgc taccttcttc gccatggatc
     1261 ccggcgactt cgaccacgaa accgcgctgg ttgcgcgggc gcaggcgcac gaagtgaaag
     1321 cgttcgaggc cttgtaccgc ctccacgtcg ggcgcatcca cgcactgtgc cggcggctgg
     1381 cgggttcgca cgcgctcgcc gaggactgca cccaggaagc cttcgtgcag gcgtggaagg
     1441 cgttgccggg ctttcgccgt gacagcgcct tcggatcctg gctgcaccgg atcgctgtca
     1501 acgtggtcat cgggcaccag cggaccgcgc tgcggcgttc ggcgtggggg cgcgaagacg
     1561 gcgacgatgc gctcgactct tgcgccgccc cggccgagcg ccccgacgtg gcgctggacc
     1621 tggaccgcgc gatcgcggag ctaccgccgg gcgcgcgcac ggtcttcgtg ctgtacgaca
     1681 tcgacggcta caagcacgag gaaatcgcgg agctgacggg actcgccgtc ggcacctgca
     1741 aggcacacct gcacagggcc cgccgcttgt tgcgggcgag gttgacagca tgaacaacga
     1801 aaccggcacc gacgaaatcc gggacgaaag gctgcgcaag gccgtgggat ccctgccccg
     1861 cgaaatcccg ccggaacacg atctgtggcc cggcatcgcg gcgcggatcg cgccggcccg
     1921 ccaccgcttc cagacttggt cgatcgcggc ggggatcgct tgcatggtcg cagccggcgc
     1981 gatcgtcacc acgctgtccc agcaccgcca gcaggcgccg gcgaccctcg ccacccggca
     2041 accggtgacc caaccgcgac cggcggccgc cacgccgttg ccgaacgagc gcgccgaaat
     2101 gctcgcggcc acgatccggc gcagcaccca cgaggatccg cacacgcagg cggtgctgct
     2161 caggaacctc gacctcgtgg aaacctcgat cacccacctc cagcaggcgc tggatgaaga
     2221 ccccgccaac ccggggctgc agccactgct ttatcaacag taccgcaatg aagccacgct
     2281 cgttgccgcc gcgcagcggg tccagcttca aaccgccaca ggagccatcg cgctatgaac
     2341 aatcacaccc accccaacgt gaaacgcttc gccctcccgg ccgggttgct gttcgcgctg
     2401 ctcgcagcca cgctgcccgc gttcgccggc acgccggtgg acaagaccgt taccgccacg
     2461 ccgaacggac acgtgcaggt atccagcgtc gcaggcagca tcaaggtcac ggcctggaac
     2521 cgcaatgaag tccacgtcac gggttcgctc agccgtaatg ccgaacgtct cgcggtcgag
     2581 aagaccggta ccggcgtcaa catccaggtc gtgcttcccc agcactcctc cggcaacatg
     2641 gaaggcagcg acctcgtcat cgaggtgccc gcggcaagcc agctcgaagt ccacaccgtc
     2701 agcgccgaca tcgatgccgg cgggctcggc ggcccggtcc gacttgaaag cgtgagcggc
     2761 aacgtcgggc tggtttccag ctcggccaac atttacgcga acacgatcag cggcgacgtc
     2821 cgcgtcaccg gttcggcgcc gggcgcaacg atcgagggcc acagcatcag cggcaaggtc
     2881 ctcatcgaaa aggtgaacgg cagcctgcgc gcgggcagca ccagcggaga catcgagctc
     2941 acgggtgaaa acagcctgca agccgcgaaa ctcgacacca cgtcaggcga cgtccacttc
     3001 gccgccgccc tgggtgcgga cggggattac gaattccact cggtgagtgg caatgtcgtg
     3061 ttgtcgctgc cgaaggttcc ggccggctcg ttcgatgtgt ccagcttcag cggcgacatc
     3121 gacaccgact tcggcccgca gccgaagcgc agcagcgagt tcgggcccgg caaggagtgg
     3181 cactaccaga gcggttccgg ccaggccgac atccgcatcc gcaccctgag cggcgacgtc
     3241 cggatcagcg cggcgcactg agtcgtaccg gccggcgggc acggcctcat gggggaagcc
     3301 gccgcgggag tggcttcccc gcggcgagcg gtgcggttac cgcgctcgct agaatggccc
     3361 gatccgttgg cgaaaaacga cgatgtcgca cgcctccatc gcttccaacc cgatcctcaa
     3421 caccgacagc tacaaggcca gccattggct gcagtaccca ccgggcgtcg atgccacctt
     3481 cttctatctg gaaagccgcg gtggcaccta cgagcgcacg ctgttcttcg gactgcagtc
     3541 gatcctgaag gagtacctgt cgaagccggt gacgccggcg atggtcgacg aggcaaaggc
     3601 gttcttcccc gcgcacggcg aaccgttcaa cgaaacgggc tggcggcacg tcgtcgatgc
     3661 gcatggcggc aaactcccgc tgcgcatccg cgcggtgccg gaaggcacgg tggtgccgac
     3721 ccaccaggcg ctgatgacga tcgagagcac ctgccccgat tgcttctggg tgccctccta
     3781 cgtcgagacg ctgctgatgc gggtttggta tccggtgacg gtcgcgacga tcagttggca
     3841 tgtgaagcag acgatccgcg aattcctcga caagaccagc gacgacccgg cggcgcagat
     3901 cgcgttcaag ctgcacgact tcggcgcgcg cggcgtgtcc agcgcggaat cggcggcatt
     3961 gggcgggatg gcgcatctgg tgaatttccg cggcaccgac acgatcctcg gggtgctggc
     4021 ggcgcgcgaa tactacggcg aggagatggc cggcttctcg attcccgccg ccgagcacag
     4081 caccatcacg gcgtggggca aggaccggga gctcgacgcc tatcgcaaca tgctgcgcca
     4141 gttcgccaag cccggcagca tcgtcgcgtg cgtgtcggac agttacgacg tcttccgcgc
     4201 ggtcaaggaa atgtggggcg gcgcgctgcg tgacgaggtg gtccaaagcg gcgccacgct
     4261 ggtggtgcgc cccgattccg gcgatcccgc ggacatcgtg cacagcaccc tcaagatgtt
     4321 gaacgcggcg ttcggcagca cggtcaacgc gaaaggtttt cgcgtgctgg accacgtgcg
     4381 ggtgatccag ggcgacggcg tgaatccgga ttcgatccgc gcgatcctgg agcgcatcac
     4441 ccgcgcgaag tattcggcgg acaacctcac cttcgggatg ggcggcgcgt tgctgcagaa
     4501 gatggatcgc gacacccagc ggttcgcgtt gaaatgttcc gccgcgcgca tcgacggcca
     4561 gtggatggat gtcttcaagg atccggtgac ggatcacgcc aaggcttcgc ggcccggccg
     4621 cctgacgctg gcgaggcatc gcgaattcgg caccttcaag accgttccgc tgccggatgg
     4681 ggcgatcgaa gcggacgccg cgacactcgg cgccggctgg gaagaagcga tgacgacggt
     4741 atgggaagat ggagcattgc tccgcgactg gacgtttacc gaaatccggg cgcgcggcga
     4801 aacctagggc gggcagtgcc cgccatccaa tggcgtgcgg cggacggtgt ccgccccacg
     4861 tgtacggacc gtcgcggtcg agcggtccat cgacgatgag caggagtcca catggatgtc
     4921 atgcacgatg ccgatgaacg ccttttccac gcggacgtgg atggccaccg cggcgaactc
     4981 gactatgtcc tgcgcgacgg cgtgatgacc atcacccaca ccggcgtgcc gcccgccatc
     5041 ggcggacgcg gcgtggccgc ggaactgacc cgcgtcgcgc tggaaaccgc ccgccgcaaa
     5101 gggtggaagg tggtgccggc ctgttcgtac gcggcggcat tcatccgccg ccaccgggag
     5161 ttcgccgacc tcctcgcctg agctccgcga tctccggttg ccggacgtcg gagtcacgca
     5221 gcttttttcg gtacactcgc ctccctacgc cttggggaag acgcgcatgc ctgccaaacc
     5281 cgctccggac aagcctgcct cggaccaacc ggcagccgga aagcccgcgg caacgaggcc
     5341 ggccgcgggc aagccgcccg agaaactgca tttcatttcc gggctgccgc gctcgggcag
     5401 cacgctgctc tcggcgatcc tgaggcagaa cccccgcttc cacgccggca tgaccagccc
     5461 gctcggcgac atgatcagcg tgctggtcgc cgaaatgagc gggaagaacg atttctcggt
     5521 cgtgatcaac gacgcccagc ggacctccgt cctgcgcggc gtgttccaga acttctacgg
     5581 cggcctccac ggcaccgatg tggtgttcga caccggacgc gtgtggtgca gcaaactgcc
     5641 gatgctggcg acgctgtttc ccaactcgaa ggtgatcgcc tgcgtgcgcg aaatgccgtg
     5701 ggtgctggac agcgtcgagc ggctggtgcg caagcagccg ctttccgcga acaagatctt
     5761 ccatttcaat ccgaacggca ccgcgtactc gcgggccgag gcgctcatcg acgtcaacgg
     5821 catggtggga ttctcgttcc aggccaccaa ggatgcgttc tatggccact tcgcgcccgg
     5881 ccggctgatg ctgctgcggt acgagagcct gacccaggat cccgggagcg cgatgcaggc
     5941 gctctacaag ttcatcggcg agccgtggtt cgagcacgat ttcgaccaca tcgaatatgc
     6001 cgccgacgag ttcgactcgc ggctgggcct gccgggcctc cacaccgtga aatcgcgggt
     6061 gtcggccccc aagcgcgagt cggtgctgcc gccggacctg ttcaaccgct tcgtcaagga
     6121 ctcgttctgg ctggatccca agaacaacat ccgcaaggtg cttgtcgtgt aggttggttg
     6181 atccccggct tgccggggag aagcccaaca tgatcgtaac gaaacacgga gattccatcg
     6241 atcaagtgcg taggttgggc tgatcccgga acgtgttccg ggagaagccc aacggctctc
     6301 cccggcagca acctacgcca gccgcgccga gcgccacaac gaagccgccg ccagcaacac
     6361 cacgaccgcc gcggccagca acgtggcgct gaagccgtgc ggcaggtgcg ccacggcact
     6421 caccagcaac gggccgatga tctggccgat cgcgaacgcc gcggtcatga ccgcgatgaa
     6481 tgcggtcgcc ccggcgcccg ccacgcggcg cgcttcgcgc aggcccagca tcgtcaccac
     6541 catgtaggtg ccgccgacca gcaacgccga aaccaggatc gcggcgaacg tcggccacag
     6601 cgcgggcagg gcacaacccg cggccatcat cacgtagcac gtcgcccaca gcttccggtc
     6661 ggcgatccgc gcgagccagc gtgcagcgat ccaggtggac agtgccgagg tggcaccaaa
     6721 caccggccac gcgagaccga aaaccagcgg atcagtgacg aagcgatgcg ccatctccgg
     6781 caggtaggtc gccggaatga tgtagccgat cccgagcgcg ccatagcacc acgccagccg
     6841 gcgctcgtcg gcggtccagc gaatgcgcgg ccgcgcgtcc tccaccgcgg gcggggccgg
     6901 cgcggcccat gggccacgga gcacccgcca cagcgccagc gtcgccagca acgacaatcc
     6961 accgagcact tgccatgtgc gatccgaatc cattcccaac tgcatcgcgg ccatcacgat
     7021 caatcccgca atggcgacgc cgatgccgac gcccgcatag aggattccgg cggcgtccgc
     7081 gcgacgcagc gacagcaggc cattcaacgc ccaggcggac acgtgcacga ggacccatgc
     7141 gctggcgatg ccggcgacga gccgcaggac cagttgcaat gcgagtgcat gggtgaggcc
     7201 catcgcgaac gtggccaccg cgatcaacag cagcccgcca cgtgccgcct ggccgaacga
     7261 aagccgcgcg gcacacagcg cgccgaggag gtagccgagg taattggcgg ccgcgagcca
     7321 acccgccgtg cgcaacgcca ggcccgcgtc ggcttccatc atcggcatca cgggggtgaa
     7381 cgcgaagcgg ccgataccca tggctacggc cagtgcggcc aggccggcga tggcgatcgt
     7441 cgcggcagca cccgggtctc cggctgcgta cgaatcctgc atccggccat tctgcgcgat
     7501 cacgcggtgc cggaccaacg gcgtcgcaat cggcaatccc ggagtcgctc agaacaacag
     7561 cagcaacgcc gggaccgcga cacccgcgaa caatgccgct ccataccgcc tgcgattgcg
     7621 cgcacaccgc cagcccatgt acacgccgct cacagtggag aggagcaggc tgagcgacac
     7681 cagcacgaag aagaccttca tcggccaggg attgcgcttg cgcggggcag gcaaaggctt
     7741 gtcggccgca cgcgccgaca taacgtcggc ctcggcgccc tgtttcgaca cggacgcgtt
     7801 ccgggaccgg cgcttcacgg gcacgtccag gctctgcttc ttgtggaggt gcgagatgct
     7861 ggcgagccag cgcggcggct gatagtcgcc aacgcgcgat gcctcgtgga gggacaagct
     7921 ctgcaacccg ccggtgaatg cgaagaacag cagcgccgga gcggtgaaca cgccgaagta
     7981 caggtgcagg gtgcgaagtg acttcagcag acgattcgtg gccatctggg ctggattggg
     8041 gaaaggaccg gatgctcgca tgaatgcgaa tcattcgcaa taataagatg gattggcgac
     8101 caccgagccc ggcccggacg aatctcatgg ggtgagaagg tggcaggcat actgggagga
     8161 ttgctgcacc ggggcctgca tgtttcgact cgtcacctcc aacaacgccg gaacgctggc
     8221 cgacgaactc ggcgacggct tgcgcgatcc gcgcggcaat ccgctcacgc ccgcgctggt
     8281 gctggtaccg caggccgggc tgcggcgctg gttgcaggtg cacctggcgg agcggctcgg
     8341 ggtgatcgcc aatgtcgaat tccgggcgcc ggcggagttc gcctgggacc ttttgcgcgc
     8401 ggcgaaacca gacctgccgc ggcgctcgcc gttcgatccg gacgtgatgc gctggcacct
     8461 gcatgcgctg ttgggggagt cgctggaagg cgccgcaatg gcgccggttc gcgaatacct
     8521 gcaatcccac ggcgatccgt tgcgccgcta tgcgcttgcc ggcgaactgg cgcatgtgtt
     8581 cgagcggatg cagggctatc ggcgtgcgaa gctgttgcgc tgggaacgcc atgaagaaag
     8641 cccggacagc gacccgcagg cttggcaggc ggagttgtgg cggcggttga tcgcacgcac
     8701 gggcggtaca tcgcgcgccg gccggatgga aggttggttg cgcagcttcg atcccgacta
     8761 ccaaccgtac gcagcggccg gcaagcccgc gccgccgggg ctgccctccc gactcacgtg
     8821 tttcgcttgc gccaacgttt cgccggacgt gctgcggatg ctcgcggtgg cagggttgca
     8881 ctgcgacatc gacttttatt tgccattgcc ctcgcgtgaa tacctgggcg acctgccgcg
     8941 ctcgcgccag gaggtccgcg agcggctcgc ggaaggttcg ggcgagaatc ccctggtcat
     9001 ttcgatggcc accgcctcac gcgaattcct cgagttgctc tacgactatg gctgcgtgca
     9061 gccggattcg gagtccgagc gcttcgacga atccatcgcg cgcgacaccc tgctcggccg
     9121 cgtgcgagac gacatcctcg cgcatcgcgc agctcgcgaa gacgatcgcg tggcgctgcc
     9181 ggacgactcg ctgcagttcc acgcctgcca taccgcgctg cgggaggtac agtccctgca
     9241 cgaccggttg ctcgcgatgt tcgcggccga tccgacgctc aagccgcgcg agatcgcggt
     9301 gatgatgccg gacgttgcgg cctaccggcc ggccatcgag gcggtattcg gcagcctgcc
     9361 cgatcacgat ccgcgcttca ttccgtacaa cctcggcgat ctcgccgcgg ccgccacgca
     9421 tccggccgca cgattgttcc tgagcctgat ggatgcgccg acctcgcgct ggcggctcag
     9481 cgaaatcctg gatgtgctcg ccgtgccggg cgtgatgcgc cgcctcgatc tggacgcgcc
     9541 ggaactggag cagttgtcgc gacaattgaa ggaaagcggc gcgcgctggg gcgaggatga
     9601 gcacgcacgt gcgcaggtcg gcggctatcg cgaattctcc ttcgcgttcg gactggaccg
     9661 catgctggcc ggtttcgcct gcggcgacga tgacgaagcg ctggtcgcgg gcgtcgcgcc
     9721 cctggcgggc gtggaaggcg cggcgttcgc acggctcgac gcgctgctgg cggtactcga
     9781 cgcctggaag cgactccgcg aattcgccgc gcggagaatg gacgccgccg aatggcagcg
     9841 cgccctgaac ggcctgttcg atgcgctcta tgcgggcgat ccgggcgaca tcgccgaggc
     9901 acgcgcgctg gagcgggtac gcggcgcact ggaggatctg gtcgaacaca cccgggcggc
     9961 gcacttgcaa cagcctttgg cgtggcacga cctgcgggca ttcctgcgcg agcgcatgga
    10021 agacgccgac ccgcgccagc aactgttctc cggcggcgtc accttctgcg gcatggtgcc
    10081 gctgcgggtg gtgccgttcc gggtgatctg cctgctcggg atggacgagg ctgcgttccc
    10141 gcgacgcgat ccttccggcc tcgacccctt gagcgccgac cgccgctcgg gccgtgccca
    10201 acgcggcgac cgcagcgtgc gcgaagacga ccgcctgctg ttcctgcaat tgatcgccgc
    10261 cgcgcgcgat gccttgtaca taagctggat cggccgcgac gctcacagca acgaaaccct
    10321 ggcgccgtcg gtggtcgtgg cggaattgat ggacgtgttg cgcgaaggct atctcgcgca
    10381 ggatgacgat tcgcgcgcat cgagcgcccc tctgctgccg cgcgtgcaac cgctgcaccc
    10441 gttcgacgtc tcgttgttcg acggacgcgc gccgcgctcg tacggggccg agtggttggc
    10501 agccgcccgc cggccggacg cgcgggcggc ggacgcgccg ttcatgccgg acgcgccttt
    10561 gccccgactt gaagctgcgc aagcggccgc gctgacgctg gaggacctca agaggttcct
    10621 gctcgacccc gcgcgcggct tcctggaaca gggcctgggc cttgcactgc cgcgagggcg
    10681 acccgccgat gccgacgagg agcccctgtc gccacgcgat ggcctgacgc gttggagcct
    10741 cacccgcgcc ctgctggatt tcggcgaagg caatgcttcc ggcgaccgcg acctgctgcg
    10801 cgcgcgcggc cagttgcctc ccggcgcgct cggcgacgag gcgctgcgcg atgcacacgt
    10861 gcaagcggac gcgttgcggg ccgtggtgct ggagttcacc cgcggcgcgc cgccttcgcc
    10921 cgcccacccc ctgcgcgtgg actgggacga cggcacgcgg ctggaaggcg cgccggccga
    10981 tccttatccg ggcggcgtgc tgcacgtgcg acccggcgaa atcgatggcc ggcacgtgct
    11041 gcgtgcctgg ctggatgcgc tgttgtgttc cgcgggcgga accggcaaac ccgtgatgct
    11101 ggtcggcctc ggtgagcagg acgcgtgctc gttcgcgctg ccgcagttgg cgcgggaggc
    11161 ggcgcggggc gaattgcgcg ccctcatcga actctgccgc gagggccggt gcgcgccgct
    11221 gccattcttc gtgcgtgttt cctggaaaca cgcgctggcg tgtgccaagg cggagaaaaa
    11281 atccggcacg ccgccggggc gggcggacat cgccgtcttc gaaaaggccg ccaagcaggc
    11341 cgagggcgac aacgggttcg gcggctccga cgaattcggg caggacgcgg tgtgcatggc
    11401 ctggcgaggc cgcgagctgc cagggccggc cgatggcgga ctcgcgcagg acctgcaccg
    11461 cctcgcgctg gcggtattcg cgcgaccggc gcgtgcatgg gcggagcagt tcccatgacg
    11521 ccgctggatc tgccgctttc gggcgttcgg ctggtcgaag ccagcgcggg caccggcaag
    11581 accttcacga tcgccacgct ctacctgcgg ctggtcctgg agcgcgggct ggtgcccggg
    11641 agcatcgtgg tcgccacgtt cacccgcgcc gcgaccgcgg aattggcgat gcgcctacgc
    11701 gcgcggctgc ggctggcgtc ggagatcctt cgcgatgcgg atcctgcgcg cgagcgcggg
    11761 ggcgatgacg gcgaaaccca ggcgacccgc gcggtgatcg cacaggcact cggcgctgcc
    11821 gatgcgggaa cgctggccgg gcgcgcgcgc gacgcggagc tggcgatgga taccgccatg
    11881 atcggcacgc tgcacgcctt ctgcaatcgc gtgctgaacg aattcggctt cgagaccggc
    11941 cgcgcgcccg gcgaactcga gctgctggag gacccgcgcg cgctgcagca ggagatcatg
    12001 gaagatttct ggcgcagttg ttcctcggac gcggacgccg cgcgcctgct cgccgaaacc
    12061 tggcgcaccc ccgaggcact cgcgaagcag gtgtgcgagc cgcgctggcg cgcgcgccgc
    12121 gtggtcgccg gcgatgccgt gcaaataccc gcgcagcagc gacgagtgca acgcgaggca
    12181 acgttggcgc aactggatgc gatccgcaac gcgatcgccg gctgggatga cgctgcgttg
    12241 caggtcgcca tgcaggaact tgccgactgc atcagccatc agggagcgcg gaaggcccgc
    12301 gcgcaaggct tgcaggcttt gcgtgactgg gcgcgggcgg gaaccgcggc ggatcttctg
    12361 tcggccgccg cgcgcaaggc ggtcgacggc ctcgccgcag acaagctttc cgaaatgaag
    12421 acgtgcacgc ggcttccgga agggcccgtg ttcgccgcga tcgccgatct cgacgccaat
    12481 atcaaatcgc tcgaacagct cgaccaggcc gcacgtgccc ggctgctgct ggatgcccga
    12541 ggctggctcg aacgtgaact gcccgagcga atgaaggcgc tgggcaagct cggccacgac
    12601 caggtggtgg atgcattggc agcggcgttg caggacccgc agcgcggcgc gcaggcagta
    12661 cgcgccattc gcgagcgctg gccggtcgcg ctggtcgacg aatcccagga caccgatccg
    12721 caacagtggc ggatcctcga gaagctgttt gcgcacgaga acggcgcgct ggtgctggtc
    12781 ggcgatccca agcaggccat ctacggcttc cgcggcgggg acgttcacgc atggctgatg
    12841 gcgaagcggg aggcgcaggg cgaaccgctg cggctggacg aaagccagcg cgccggcgcc
    12901 ggcgtcaacg cagcgatcaa tgcgctgttc tcgggcgaca attccttcgt cgaaccgggc
    12961 atcgtccacg aggacgtgca tgccgcgccg cgcgtcgccg aacgcgcgtt gctcgtggac
    13021 ggcgagccgg cacccggact gaccgtctgg cgtgtgccgt ccaccggcat caccaacaag
    13081 gacggcggca cgcgggcctg gtacaaaggc gacgcgcaag cgcggatcga aaccgcctgc
    13141 gtggcgcaga tcgtcgattg gctcgagggc gcgcgccggg gccgcgtgcg tttgcgcgaa
    13201 ggcagtggcg actcgcgtga gctgtgcccg cgcgacatcg cgatattggt caacagcaac
    13261 cgcgaggcga aatccatgca gcgcgcgctc ggtgccgccg gggtaccggc tgcgtcctgc
    13321 ctccgcgcca gcatctacgc cagcggcgaa gccgccgacc tgcgcctgct gctgcaagcc
    13381 ttgcgcgacc cgggcgatgc cgcgcgtgcg cgcgccgcgc gcgcttcgtt gctggtgggc
    13441 gacgatgctc tcgcgattgc acgcacctgc agcgacggcg ctgcgctgga tgcgctgttg
    13501 accgaaaccg ccgagtgggc cgcgctggta cagcgacgcg gtccgctgcc gctgctgcat
    13561 cggctggtcg cgcgtgccgc gccacgcctg ctcgcagcgc ccggcggcga gcgccgcgtc
    13621 gccaactacc tgcaactcgc cgacctgctg caggacgagt acgcgacctg cttcgggatc
    13681 gacgacctct gcgagcacta cgcgcgcgca attcgggaga gcgggaacga caccgatgcc
    13741 gacgcggtgc ggctgcgcct ggacaccgat gcgcaggcgg tgcagatcgc caccgtgcat
    13801 gcggccaagg gcctggaata cagcgtggtg ctgctgccgt gcgcggtgtc gggccacgga
    13861 aatggcggca gcggcaggaa gcccgcgctg acctggcatc acgaaggtga cagggcctgc
    13921 gtcgcagtcg gcagcggggt cgctgcggag atcgaaacgc tggcgcaccg ggaagccctt
    13981 gccgaggagg tacgcaagtt ctacgtggcc gtgacgcgtg cgcgcgcggc ctgcttcatg
    14041 ccttggggct gcgtcaggca agcggatgaa accgccgcgc actggttgct ccatgtggcg
    14101 aggcgcgagg cgccactcgg ctgcgatgag cctgcatgcg aacaggccct gtccgaactc
    14161 gtcgcgcggg gcagtgggca tgtcgccgtg tcgccgctgc ccgaagcacg ctcgaagcgc
    14221 ttgtcgaagg cgcgatccgg cacaacgcgg atcgaagccg cacggttcca tggcgacgtt
    14281 gaacgtgact ggcgcgcctg gtcgttttcg cggctggtgc gcggcgaggc tcgcggcgcc
    14341 gcagccgatc ccgcgcccgg ccacggcgac tcgccttcgg ccgccggcga cgcacggccg
    14401 caggccgcgc ccatgctggc cggcgcgcgc tttggcagcg cggtgcacgc ggcactggag
    14461 cgcgccgatt tcgacgcgtg gcgcggtaca gacggaattc ccgagctgca acgcgacctg
    14521 atcgctcgca gcctgcgcgc gcagggcctg cccggccccg gcaccatttc catggagcgg
    14581 gcggtgaccc tggtcggcga gtacatgcga gccgcgctga acgtggcgct cgcctgcggc
    14641 acccgcctgt gccacatcca accagaacgc cgatgcgcgg aaatggaatt ccatttgcgg
    14701 ctcgcgccgt cgcgagtgga cgcgttgttc gcgctgcttc acgaacatgg ataccagcgc
    14761 gggcggagcg ggttcggcgt ggggcgtctc cacggcctgt tgaccggcgt gatggacctg
    14821 gtgttcgagc acaacggccg ctatcacctc gtcgactaca agaccaacct gttgcctgcc
    14881 tacgacgccg gcgcgctgtg cgaagcgatc gccacccacg actacgacct gcaatacctg
    14941 ttgtacgtgc tggcgctgca ccgctggctg cgccaggcag tgcccggcta cgactacgac
    15001 gcgcacgtcg gcgacgtgta ttacctgttc gttcgtgagc tgacggacgg ccgcggcgtg
    15061 caccgcgatc gtccgccgcg tacgctgatc gaggcgatgg atgcgctgtt cgacggcgaa
    15121 gtggagcagg ccgcatgagc gccgacctgc aagcgcttgc ccgcgccgga tggctgcgcc
    15181 gcgtggatgc agcactcggc gaatgggtcg cgggcgcgtt tccggatgcg ccggccgaag
    15241 tcgcgcttgc tgccgcgctg gccgcgcggg cggtgggcga aggccacagc gcattgcaac
    15301 tggatgcggc acaagcgtgg cttgggcacc tcgatggccg gggcgatggc ccggcactgc
    15361 ccgatccggc ggcctggcaa gccactctgc gcaccagcga agcggtgcat tgcgggtcga
    15421 gcctggacgc gaacgaccct gtaccgcccc gaccgctggt actggatgcc gaaggacgcg
    15481 tgtacctcgc gcgttacttc gattacgaac ggcggcttgc acgaaatctt gtcgcacgtg
    15541 cgcatacgga acgcctgaag ctgaccaccg gtggtcccgg caccggcaag acccacggcg
    15601 tggtgcgcct gctcgcaacg cttgcgggca aagcacacgc gcaggcgcgc ggcttgcgta
    15661 tggcgctggc cgcgcccacc ggcaaggctg cggctcggct ctcgcaaagc gtccgtgcgc
    15721 agttgccacg gctcggacta cccggcacgg tcgacgcgat gattccgcgc gaggcttcca
    15781 cgttgcatcg cctgctcggc ctttctcctg ccaatgcgcg ggcgaaattc caccgcggtg
    15841 cgccgctgcc gtatgacgtg gtggtggtcg acgaggtctc gatggtggac ctgccgatga
    15901 tgtacaggct ggcggacgcg gtgcgcgacg acgcgacgct ggtcctgctg ggtgaccccg
    15961 gccagctcgc cgcggtggag gcgggcgacg tattggcggc actggtgacc gccgcgcgcg
    16021 agccgccgct gtcgcgatgc catacccacc tcacgcagag tcggcgcttt gcgggacacg
    16081 gcgcactcgc gcgactggcg aacgcaatcg caggcggcga cgcgcaagcc gcgctggcca
    16141 cgttgcaaga agccaatgac gaagtgcggc tgctcgcgga tggcactcgc agcgcccggc
    16201 tggtcgagga cgctgcggat tcataccgca ccgtgctgga tgcagcggat gccggtgcgg
    16261 ccctgcgcgc ggccaatggg ttccgggtgc tgaccgcgct gcggcacggc ccggcgggga
    16321 acctcgcgct ggaccacgca atcgagcagc gcctgaagcg ccgcgccggc gtgcgtgccg
    16381 atgcaccgtg gtggcccgga cgcctgctga tggtgacggc gaatcgcgac gggctcggcc
    16441 tgttcaacgg cgacgtaggc gtggtctggc ctgacgccaa tggcgagatg aaagtctggt
    16501 tcgaggggac cgagggcgcg ccacgcgctg tttcgaccgc cgcgctgccg ccgcatgaag
    16561 gcgcattcgc gttgtccgtg cacaaggcac aagggtcgga attcgggcgc gtcgcgctgg
    16621 tgctcggcgc ggacacgccg gtgctgaccc gcgagctgct ctacaccggc atcacccggg
    16681 cgcgcgaagg cgtggaaatc catggcgacg aagcgctgct gcgcgccggc atcgcacggc
    16741 gcacgcagcg ctggaacggc ctggccgacc gcttgcgcga ggcggcgaat gcccccattt
    16801 cgattgcgga atctttaccc gggccgacat agactttcgc ttcggccgtt gcggacgtgg
    16861 cacggccgcg atccggaatg acgacaagac agaaggttcc cgttccccat tccccgttcc
    16921 ctgttcccgc ttcggcacat gcacgccttc catcccgccg tctccgcctg gttcgagtcc
    16981 gcgttcgatg cgccgaccga ggcgcaggcg cgggcgtggc cggcgatcca gtctcaccag
    17041 gatgtgctga tcgccgcacc gaccgggtcg ggcaagacat tggcggcgtt cctcgccgcg
    17101 atcgaccaac tggtgcgacg cggcttggaa ggcacgctga acgacgaaac gcatgtcgtc
    17161 tacgtgtcgc cgctgaaggc gctgtccaac gacgtccgga tcaacctgga agtgccgatc
    17221 gaaggcatcc gcgccgaact cgaacgcacg ggcctgccgg atgtcgagat ccgcaccgcg
    17281 gtgcgtaccg gcgacacccc gcaggcggag cgcaccgcga tgcgcaagcg cccgccccac
    17341 atcgtggtca ccacgccgga atcgctgtac atcctgctcg gttccgagtc cggccgcgcg
    17401 atgctcgcca ccacgcgcgc ggtgatcgtg gacgaaatcc acgccctcac gcccaacaag
    17461 cgcggcagcc atctttcgct ctcgctcgcg cggctggatg cgctgtgcgg ccggcgcccg
    17521 gtgcggatcg ggctgtcggc gacgcagaaa cccatcgagg aagtcgcccg attcctttgt
    17581 gcggatcgcg attgcacgat catcgacgcc ggccaccagc gcgcgcggga cctcggcatc
    17641 gaggtcacca acgtgccgtt gcagccggtg atgagcaacg atgcctgggg gctggtgtat
    17701 gaccgcatcg cggaactgat cagccaacac cgcaccaccc tgatcttcgc gaacacgcgg
    17761 cgcacggtgg aacgcgcgac gcggcatctt tcggaacgcc tcggcaagga ggcggtggcg
    17821 gcgcaccacg gcagcctttc gaaggaaaaa cggctggatg cggagcggcg cctgaagtcg
    17881 gggcagctgc gcgcgctggt tgcgaccgca tcgctggaac tgggcatcga catcggcgaa
    17941 gtcgagctgg tctgccagct cggctcgccg cgctcgatcg ccgcgttcct gcagcgggcg
    18001 ggtcgcgcgg ggcacgcggt cagcggcacc tcgaaagcac ggttgttccc tacctcgcgg
    18061 gatgaccttg ccgagtgcgt cgcattgctc gattgcgtgc ggcgcgggga gctggacacg
    18121 ttgcacgtgc cgcgcgcacc gctcgacgtg ctggcgcagc agatcgtggc ggaggtggcc
    18181 tgcggggagc gcgacgagaa cgaactgttc gatctggtgc gcagcgcgta tccgtatcgc
    18241 gacctcgccc gcgacaagtt cagcgaagtg ctgcggatgc tggccgaggg ctacagcacc
    18301 cggcgcggca cgcgcgcggc ctacctgcac cgcgatgcgg tgaacggcaa gctgcgcgcc
    18361 cgccgcaacg cccgcctgac cgcgatcact tccggcggcg cgattcccga caccgccgat
    18421 tatgacgtgg tgcttgaacc cgcggcgcag cgcgtgggca gcgtcaacga ggatttcgcg
    18481 gtcgagagca tcgccggcga catcttccag ctcggcaacc agtcgtaccg gatcctgaag
    18541 gtcgagcgcg gccgcgtgcg cgtggaggat gcacacggcg cgccgccttc gattccgttc
    18601 tggctcggcg aagcgcccgg gcggagcgat gaactgtcgt gggcggtatc gcggttgcgg
    18661 gaagagattt cggcgcggct atcagaaagc cctctccccc aaacggctgc atcgtttggg
    18721 gagaggcggc cgcaggccga gcgcgtagcg ctggtgaggg ggcaggatgt cgccgaatcc
    18781 ggcggctccg cggctgaccg aagcctcacc cccctcaccc cagccctctc ccccaatgaa
    18841 agcgttgggg gagagggagc acaagccgcg ctcgtctggc tccgcgacga ggtcggcgtg
    18901 gtcgagcccg cggcaaaaca attgctcgag tattaccagg ccgcgcgcac cgaactcggc
    18961 gtgctgccga cgcatgccat gctcgcgatg gagcgcttct tcgacgaatc gggcggcacc
    19021 caactggtga tccattcgcc ttacggcagc cgcctgaatc gcgcgtgggg cctggcgctg
    19081 cgcaagcgct tttgccgcac cttcaacttc gaattgcagg cggcggccac cgaagacgcc
    19141 atcgtgctat cgctctcgac cagccacagt tttccgctgg aggaagtttc gcgttacctg
    19201 cattcgaaca gcgtgcgcgg cgtgctgacg caggcgctgc tggatgcacc gatgttcgcg
    19261 ttgcgctggc gctggaacgc cagctccgcg ctggcgctgc cgcggttcgt gggcggcgcg
    19321 aaggtgccgc cgcagatcca gcgcatgaaa tcggaagacc tcctggcgac cgtgttcccc
    19381 gaccaggtcg cgtgcctcga aaacatcgtg ggtgaacgca agattccgga ccacccgctg
    19441 gtcgagcaga ccctgcagga ttgcttgtac gacgcgatgg acctggaagg tctggtcgcg
    19501 ctgctgagga aactcgaggc cggggaagtg acgatcgtag cccgcgatct caccgcacca
    19561 tcgtcgttgt gcgcggaagt gctgggcgcg cggccgtacg cgtatctgga tgatgccccg
    19621 ctggaagagc gccgcaccca ggccgtggtt tcgcgtggct tcaccgacgc aggtcgcgcg
    19681 gaagacctcg gcaagctcga cgcggacgca atcgccgcgg tgcgcgagga agcgtggccg
    19741 caaccgcgcg acgccgacga gatgcacgaa gcattgctgg cgctcggtgc cattacggat
    19801 ggcgaagctg cgcacgatgt cggatggaac gaccatctgg aagagttggt gcgggcgaac
    19861 cgggcgaccc ggatgctgct caagctccct ctcgcccgca aaggcgaggg cgagagggct
    19921 tgtttgtggg tcgccgccga aatgctgccg atgtggcgcg cgatccatcc ggatgcggcc
    19981 atgcaaccgg caatcgaggc accggcggaa ttcgccgcaa ggaactggtc gcgcgaggat
    20041 gcgctgctcg atatcgtgcg cggaaggctg ggctgtgtcg gaccgacacg agtcggcgaa
    20101 ttggcacaaa cgttcggcgc aagtgacagc gacatcgcca ttgcgctcgc cgcgctggag
    20161 cgcgaaggcg gcgccatgcg cggtcgcttc accccgggcg cgtacgacga ggaatggtgc
    20221 gaccggcacc tgctggcgcg tatccatcgg cgcaccctgc agcgcttgcg tcgcgagatc
    20281 gaaccggtag cgccgcgcga cttcatgcgg ttcctcgccg actggcaaca cgtttcgccg
    20341 ggtgcgcgcc tgcgtggccc cgacgcattg gccggggtgc tggcgcaact ggaagggttc
    20401 gaggccgcgg ccggtgcctg ggagtcgcaa ctgctgcccg cgcgcgtggc cggctacgac
    20461 atcgcatggc tggacgccct gtgcggatcg ggacgaatcg cgtggacgcg gctgcgcgcg
    20521 gcatcggggg gtgcgcatgc gtcccccgtg cgcgcttcgc cgatcgtgct gctgccacgc
    20581 aagcagctgg cgacctggaa tgcgatcggc gcgcagtctg cgggcgaagc gcccgccttg
    20641 tcgtcacgcg cgcaagccgt cgtggaattc ctctcccggc atggcgcgtc gttcttcggg
    20701 gagattgccg acggcacgcg tttgttgaag gtggagctgg aagacgcgct gggcgaactg
    20761 gtcggcgccg gcatggtcgg cgcggacagc ttcgccggcc tgcgcgccct gctgttgccg
    20821 accgccaaac gccggggcgc tcgccagcgt cgactcgcgc ggcacatgtt gaacggtatc
    20881 gaagatgcgg gacgctggag cctgatccgg cgcggcgacc ccgcccctcc cgcgcaccca
    20941 ggcgagggag aacacatcga gcacatcgcg cgcacgctac tgcgccgctg gggcgtgttg
    21001 ttctggaagc tgctgcaacg cgaggccgcc tggctgccgc cgtggcgcga tctgcgccgc
    21061 gtctaccagc gcctggaagc gcgcggcgaa atccgcggcg gccgtttcgt ggaaggcatg
    21121 gtgggcgaac agttcgcgtt gcccgaagcc gtggaggtcc tgcgtcgcgt gcgcaaacgc
    21181 gaacacgacg gagcgctggt agccgtcagc ggcgcggatc cactgaacct tgtcggcgac
    21241 gtggtcgccg gcccgagggt gcccgcggtc gccgcttcgc ggatcgctta ccgcgacgga
    21301 ttgccggtgg cgatccgggt ggccggggaa gtcacgttgc tggaagcgct gtcgccggaa
    21361 gaagaacgcg cgctgcgcgc acgcatgcgt gcggtcgttc ccgatcgccg gcttgtccag
    21421 cctctcgcgt aacccggcgt cgccggtcag ggaagtcccc ggcgccatcg aaggcgtcgc
    21481 aacagcgatt tcgggcgggc ccccgtctat ttcgccttga tgatctgcga caggtaatcc
    21541 ggagcgccct cgatgcgcac caggtgcgga tactgcgggc cgccgtggta atcgaccggc
    21601 acggtcttgt agtggccctg gtattccagc agcaattcga tcggcgactt gtcgtcctgg
    21661 gcggccttga tcgcatcggc cagcaccccg gacttgtagg cctggccgtt gaccgccacc
    21721 agcttctcgc ccgaaccgac gccggccttg aaggcgggac cattccagcg cacgtcgttg
    21781 atgtcgccat ccttgccgat ggcaaggccg atcgagaaca ggaagctgcg ctgggcgtgg
    21841 tagcgcttgg cgatctgctt ctggaaatcg ctttcctcgc cgttgtagac cagtttccag
    21901 cccgccgcct cgatgccgtc cagcggcggt tggtgcgcgt cgaggcgcga gcgcaggaag
    21961 ttggcccagt cgtacttcgc gacgccgtcc agcgcggcca ccaggtcgtc gaacgtgtac
    22021 gtgctggtca ggaagctgcc gttgtcgacg ccgaagaagg ccttggcgaa ggtgtccagc
    22081 gacttgctgt cgtgggtcag cgagcggatc ttcgcatcca ccgccagcca caggagctgg
    22141 cccgcggagt agtactcctc gctcatctgc cagttgcggt acggcaacgg cgcgcgctgc
    22201 gcgatggtcg gatcgttggt ggtgtcctgg agcgtgcgcc aggcgaagcc cgtgcgaccg
    22261 cggtcgtacc tcgccgcgac cagcgccagc gcttccttga actggtcggg cgtccagagg
    22321 cccgagcgcg cggtcaacac gtagccccag tactgtgtct ggccttcgta cacccacagc
    22381 agcgaatcgc ccatcggcac gttgaagttg ggcgtccaca gatcggccgg gcgacggaac
    22441 ttgccgttcc aggaatgcgt gtattcatgg gccagcaggt cgcgcccggc cacgttgttc
    22501 ttccagtcgg tgaagtactc ggggcgcgcg ccgtcctcgc tggactggtg gtgttcgagg
    22561 cccttgccgc tcaactcatc cgacagcgag aacaggaagt cgtagtggtc gtaatggtgc
    22621 gagtggaaca gcttgtacgc ctgggccacg aggttgcgat gcaactgcaa ctgctcgggc
    22681 gtgatctcga ggtctttcgg cgcgtcggcc acgatgtcca tgtgcaccgg caccttggcg
    22741 cccgggtcga ggtccacgcg cttgaaatac ttgcccgcgt acatcggcga gtcgaccagc
    22801 gtgttgtagg tggtcggctc gaacgtcgtg gtattgcccg actgcgactg ccggtcctga
    22861 tgggcaactt tcagcgccgt tccgaactgc cagccggcgg gcagcgtgac gctcggcgcg
    22921 aaggcgatgt cgcgggtgta gtgcccggcg gggtacagcg acaccttgct ccatacgagg
    22981 tccagcatct tgccggtcat ttcgatcggc ccctcggcgt gcgaacgcgc cgacaggaac
    23041 tggaattcca cgtcgatgct cgacacgccg tgcggcacgt cgacatggaa cgcatggacg
    23101 tcgtacttgt cgcgggtcca ggcgagcggc ttgccgcctg ccgtgatcgt gaggcccgcc
    23161 accttgtcga tcggccccgt cggcgaatga ttgccgggga tccatttcgg atacagcagc
    23221 gtcaactcac ccgcttgcac gggaatggtt tcgtgcacgc ggaagatgcc ctggctggtg
    23281 tcgctggcat ccacgacgat cttgatggtg ccggcgtagg gcgtgtcctg cggcggcggg
    23341 atgcgtgcgt cggcggcatc ggccatggcc gtgccggtga acgacgcggt cgcgagcgcc
    23401 gcggccagcg cggcggtgag gcaaagggtg cgtcgattgt gtgtattcga tggcgccatg
    23461 gcgttgctcc cgctcacttc ctggccttga tgatctgcga caggtaatcc ggcgtgccct
    23521 tgatccgcac gaggtgcgga tactgcagtc cgccgtggta atcgaccggc acggtggcgt
    23581 attcgccctg gtacttgatc agcaattcga tcggctgctt cgaatccctg gccgcggtga
    23641 tcgcctgctt caacacggcg gccttgtaat cctggccgtt cacggcaacc acttccccgc
    23701 ccgagccgat cccggcctcg aatgcgggac cgccccagcg cacgtcgttg atgccgccgt
    23761 ccttggtcag cgtgagtccg atcgaatacg tgaagttgta catgtgccgc ggcgattcgt
    23821 cctggctttc gtactgcttc tcgtacggac tctcctcgtc ggtatagacg agcttccagc
    23881 cagtcgcctc cagtcccttg gtgaagggcg gctcgagctc atcgacgtag cgatcgagga
    23941 agcctttcca gtcgaacttc acgacgccgt ccagtgcggc gacgacgtcg tcgaaggtgt
    24001 aggtgttcgt gacgaagctg ccgttgtcga caccgaagaa ggccttggcg aagtcgtcca
    24061 gcgacttgcg gccgcgggtc agcgagcgga tcttcgcgtc caccgccagc cacatcatct
    24121 ggccgcccga gtagtactcc tcgctcatct gccagctccg gtatggcagc atcgcgcggt
    24181 gcgcggcggt cggatcgttg gtggtgtctt ccagggtgcg ccactggaag cccggccggt
    24241 tgcggtcgta gttcgcggcg accatcgcca gcgcatcgcg gaactgttcc ggcgtccaca
    24301 tgcccgcacg cgcggtcagc acgaagcccc agtattgggt ctggccctcg tacacccaca
    24361 acagcgaatc gcccatgggc acgttgaagc tcggcgtcca cagatcggca gggcgacgga
    24421 acttgccgtt ccaggaatgc gtgtattcgt gggccaggag atcgcggttg ggagcgttgt
    24481 cgtcccagtt cgtgaagtag tccggcccca tgccgtcctc gctggactgg tggtgctcga
    24541 ggccgttgcc gctcaactgg tccgacagcg agaacaggaa gtcgtagtgg tcgtagtggc
    24601 gcgagccgaa cagcttgtag gcctggctga ccaggttgcg gtgcacctgc aactgcccgg
    24661 gggtgacctc gaggtccttt ggcgcgtcgg cgaacacgtc gaggtgcacc ggcaccttgg
    24721 cacccggatc gaggtcgagg cgcttgaagt acttgccggc gtagatcggc gaatcgacca
    24781 gcgtgttgta ggtcgtcggc tcgaaggtga cggtgtcgcc cgatcgcgac tgttcctcga
    24841 gcgctgtgcc gaactgccag ccggccggga acgtgacgct gggcagcacc ctgatctgcc
    24901 gcgagaagtg gccggccgga tacagcgaca cggcgttcca ttccagcgac agcatcttgt
    24961 cggtcatctg gatgaagccg aacttgccgc gccccgagag gaactggaag tccacgtcga
    25021 tgctggaaac gccctgcggc acgtccacgc ggaacgcgta cacgtcgtac ttgtcgcgtt
    25081 tccacctgag cggctcgccg ccggcggcga ccttcaatcc cgcgagcatg tcgatcggac
    25141 ccgacggcga atgggcgccg ggaagccact tcgggtacag cagcgtcagt tcaccgggtt
    25201 cgaccgggat cgtttcgtgg acgcggaaga tgccctgccg ggtgtcgctg gcgtcgacat
    25261 ggacctggag cgtgccggga tacggcgtgt cctgcggcgg cgggatgtgc gcgtccgcgg
    25321 cgtccgcgaa cgccgcgccg ccgaagccca tgccggcgag gctcaacgcg aacagggcgg
    25381 ccaggcgaac cggcgaggac caacggcgat gcagaaacat gcgggcactc cgcgagtatg
    25441 taaaccgtcg aacctaacac cgcccgcgcc ggcatgcata ggccggatgg cacaggcgcg
    25501 gtcagcggct gcgcgcgccc tgcccgaaca ggatcttctt ttcgtcgtcc gacatcggct
    25561 ggccggcgga cggattggcc tccgttgcgc gggcatacgc acgtgccgtc gcggggcgtg
    25621 cggcgatcgc atcgcgccag cggcgcacgt cggcgaatgg ctcgaggtcg agcggcgcct
    25681 tcgaatacgg atcgatccac ggatagcacg ccatatcggc gatgctgtag tcgtcgccgg
    25741 tgatgaatgc gcgcccggcg aggcggcgat ccagcacgtc gaggagccgc tcggcttcct
    25801 tgcggtaacg ctccatcgca tagggaagct tctccggcgc gtacacgtgg aagtgcccgt
    25861 actgcccgag catcgggccg aggccgccca cctgccagaa cagccattcg aggcactcga
    25921 cgcggccgcg cgcatccgcg ggaatgaact tgcccgcctt gtccgcgagg tacatcagga
    25981 tcgcgccgga ttcgaacacg ctgatcggcg cgccgccgcc ggcgggatcg tggtcgacga
    26041 tcgccggcat cttgttgttg ggcgagatcg caaggtagcc cggttcgaat tgcgcgccct
    26101 tgccgatgtc cacgggcttg atcgcgtagt cgacgccggc ctcttcgagg aaaagcgtga
    26161 ttttgtggcc gttcggcgtc ggccagtagt acagatcgat catcgggagc acctcgcaat
    26221 ggtgaacgcg gcgccgcagg gacggccggg cgcggcagac atcaccgaga tggtgtcgcg
    26281 ccgccgcccc cacaagcccg ctcgccgcga cggctcattc cacgcggttc agcgcgctat
    26341 ggcgaatccc gtagccgaaa tagatcacga agccgatcag catccagacg aggaagcgga
    26401 tgtaggtgtc gcgatcgagc ccgccgatca ggtggaactc gccgttcgtg acccacggcc
    26461 agccgatgat caggaacagc gagaacacca cgcccagggg cgccagcagc cacaaggcgg
    26521 gggtacggaa gcggcgttcc aggtcgggcc gacggatgcg cagcaccagc accgaggtgc
    26581 agatgaggat gaacgccgac agcgtgccga tgttgaccag cttggccagc tcggtcagcg
    26641 gcacgaagcc ggcggtgacc gcggtggcca cgcccaggat cgcggtggtg cgatacgggg
    26701 tgccgtattt cgggtgcgat ttggcgaacc agcgcggcag cagcccgtcc cgggacaatg
    26761 caaaccagat gcgcgcagcg ccgagcatga acgcgaacac cacgctggcg atgccgcata
    26821 ccgccgccac gttgatgatg ccgccgatcc agtgcagccc gcgttcctgg aaggccgcgc
    26881 tcaccggtgc cggcacgttg agatcggtgt agtgcaccat gccggtcagc accagcgaca
    26941 ccccgaaata catcgccatc gccaccacca gcgacagcac gaccgcccac ggcaggtcgc
    27001 gctgcggatt cttggattcc tcggccgcgg tggtcagggt gtcgtaaccg aaacaggcga
    27061 agaacacgat gctggccgcc gccatcacgc cggcccagcc gtactgcgag gtgccgtcgg
    27121 catgttcgac caccgccgga acgaacgggt gccacaagtc cgggttgacg tagaacgcac
    27181 ccaccacgat cacgatcacc acgccgacca ctttcagcgc aaccaccacg gtgttgaagc
    27241 gggcgccgat ttcggtgcgg aagatctgca ggccggcgat cagcagcgcc accaccgccg
    27301 cccacaggtt gaacatcatg ccgtcgcctt cgccgtaggc gccctgcagg tacacgggca
    27361 attgccagcc ggtgatctgc tggatcagcg actgcaggta attggacgtg ccggcggcca
    27421 ccactgccac gatcaatgca tattcaatca gcagatccca gccgatgatc caggccggca
    27481 attccccgag caccgaatag ccgtaggtat aggcgctgcc ggtgaccggg atcatcccgg
    27541 cgaattccgc ataggacagt gccgcgcatg cgctgccgat gcccgcgatc aggaagctga
    27601 tgacgatcgc ggggcccgcg tgcaacgcgg cttcgtgcgg cgcgagcacg aacacaccga
    27661 cgccgacgat ggcgccgacg ccgatcgcgg tgagctgcca cacccccatg gtgcggcgaa
    27721 aatcctggct gccgcccgcg tcggcctgca gttgctcgac cgatttgtgg cggatcatct
    27781 tctggaacag gttcatcgcg tagccccggt tgcgctagaa ccgaccgagg ataacggcac
    27841 agcctgccgg ttgggtgtgc cgatggtccc cggcgatgcg ctgcggcttc cgctccagcc
    27901 aggccggagg tgcaggctcc gcggccgccg tgcgacgata ggcacgaggt cgatccacca
    27961 cgacgccgcc cactccatgc cccgcaagcc cgcccgcacc cgacgcgaat cccggcccgc
    28021 gcatcctgta acccagcagg aattgccgct gctcgacctg cgggcgccgg aattgcaacc
    28081 ggccggggcg cccgacacct cgcgccccgc cgacgcctcg ccgcgttcca cgcgcacgat
    28141 cgagttcctg ctgcgggcgc tgccgatcgg ctggcgcgac aaggccaggg atctgctggt
    28201 gctggtgcgg atggaccgtc cgatcggcgc gctgctgctg ctgtggccga cctggtgggc
    28261 gctgtggctg gcggcggacg gattcccgcc gtggaaattc ctggtcgtct tcaccgtcgg
    28321 cgtgttcgtg atgcgcgccg cgggttgcgc gatgaacgat ttcgccgacc gcaagctgga
    28381 tccgcgggtc cagcgcaccg ccggacggcc gatcgccgcg ggacgggtga agccgcgcga
    28441 ggcggtgatg gtgttcgtcg ggctgtgcct gttgggcttc gcgctggtgc tggcgttcac
    28501 caatacgctg acgatctacc tttcgttcgc gggggccgcg ctggccgcgc tgtatccgtt
    28561 ctcgaagcgc tacacgcacc tcccgcaggt ggtgctgggc gcggcgttcg gctggggcat
    28621 cccgatggcg ttcgccgcga tcacgaatgg actgccgccg gtgtgctggc tgctgttctt
    28681 cggcaacgtg ttgttctcga ccatctacga caccgagtac gccatggtcg atcgcgaaga
    28741 cgatcttcgc gccggcgcga aatccaccgc gatcctgttc ggtgatgcgg acctgccgat
    28801 cctcggcatc ctgatggcga cgctgttgtt caccctgctg ctggtgggcc agcgcgcgca
    28861 gctcgaatgg ccgttcctcg ccgggctcgg cgtcgcgctg ctgctgttcg cctggcaact
    28921 ctggagcatg cgctggcgcg agcgcgatgc ctgtttcgcg gcgttccgca acaacaactg
    28981 ggtcggcggc atggtatggc tggggatggt ggtcgcgctc gcgatgaagt gaacgccgcg
    29041 gtgcccgctc ccgtctcgaa ttcgtttcgc tcagcccgcg gccatcgggc tctcgcagat
    29101 gagcgacaag gagcttcgac acggataaac gcggagaagc accaatcaat gaactccata
    29161 tccgagttca tcggcgttga tccgcgtcca ggaagtttct gacgagcgaa tcaagtcact
    29221 tgtttgcaat caccgtcacg gctacggtga tggcgcgatc aaccgctcca tttcggcctt
    29281 gaggatcgcg ccgtgttcgc gcagccagtc gcgctgcgcc tggtaaccgg gcatcaacga
    29341 ttcgacccgt gcccagaagc gcggcgcatg gctgcggacc ttcaggtgac acatttcgtg
    29401 caccagcacg taacgcagcg cggccggcgg cgccaatgcg agggcaagat ccagggtgat
    29461 gcggtcgcgg gcgtcgaggc tgccccaaag gctcttcagt ggcttgatcc gcatgccggt
    29521 cggcgcgacg tccagccttt cgaccgcgtg cgccatccag cgcgagaggt cgcggcgcat
    29581 ccgcgtttcg aggaatgtcg ctagcaggtt gcgcgcggcc ggcatcgcgt gggtgaacgg
    29641 gcgcggtacg tgcaggcgga gttcgtcgcc gcggtcctcg atgcgcgggt actcgctttc
    29701 ccgccaggta agctcgacgc tttcgccgcg cagcgggatc agcgtgggca ccccggcgcg
    29761 cagcggcggc ggaccgcggc gcggcacccg catttccgac agcttgcgtt ccagccagtc
    29821 gctgttttcc cgcaggaagg agtagatctg cgccgggtgg gtaccgcgcg gatacgaaac
    29881 ccgcgcgcca tgcggcgtaa ccgacaggcg cagccggcgc gcgcgcgggt gcatggcttt
    29941 caggaccttg atggtcccgc ccctgcccgc gagttccagc cattcgcccg tcattgtcgt
    30001 gcccgatgcg gctccgcaaa ccgctatgcg tccttcgcgt ccgggcgttg cagcccgaac
    30061 cacttttcca aaccggcaaa caaccgctca agttccagcg tcatcagcgc gaagcgcgcg
    30121 tccagctccg cttccgccga atcgcgcccg tcggcgtcga gcgaatcctg cacgacgtcg
    30181 agaaatcgca acttgcggat cacaagatcc tcgccgagcg tgaagctggc gcggtcctcg
    30241 aactgcaagc cgagctggaa cacctgcttg ccctgtttca gatgcgccct gacctcggtg
    30301 ctttcaagct cctggcggcg gcagcggatc accgcgcccc ccgcgggatc gcgcagctcg
    30361 cattcgtcgc cgaaggccag cccctccggc agcttgccag aggcgagcca atcagtcatc
    30421 agggcccgcg gggtctcggc cgccgccagt ggctgggccg ggaagctccc gagcgcctcg
    30481 cgcagggccg tcaaggcccc ctccgcggcc ttgcggctgg ccgtgtcgag gaccagccag
    30541 ccgctcttgg cctccaggta gacgttcagc cgcgagggcc gcacgaacgc ctggggcagc
    30601 agggtgtcca gcacctcggc cttgatggcc cgccgctgcc ggccgccgac cggccggccc
    30661 cggcgcgcgg cctccttgcg cagccgcgcg cccagctcgg cgttgacgac cgccgccggc
    30721 agcagttttt cctcggtgcc cagggtcacc agcgtggcct tgccgactgc gtggctcagg
    30781 acctcggaat ccggcccgaa gggcgacacg aagccacggg tcgcgacctc caggggcccg
    30841 caggcgcgca agacgtggtc ggccagggcc gcgtccaggt ccttcaggga tttggccgcg
    30901 gaggccgaga aacggaacag cgtgaggttg cggaagaaca tggtggagcc gggattggga
    30961 tgggtcgtgg gggtgcaagg acgggcttcg gcggaacgcg cgcagggggg ctcgcgcccc
    31021 gttacccgtc ccccgcgccc gcgtcgcccg aaagccaggc atgagtgtcc ggtcgcgctg
    31081 tctcaccccg cgcgccgagc ccggccaaat cgaacagttc ccgatcggcc aactgcgacg
    31141 gcgcgacgtg gcggagcgcc cggaacatgg tctcgctgcg gccgggatgc tcccgttccc
    31201 aaccatccag catcttgcgg acctgcctgc gctggagggt ctcctgcgaa ccgcacaggt
    31261 tgcaggggat gatcggaaac gcgcgttcgg tggcgtactc ggcgatctcg gactcacggc
    31321 agtaggcgag aggccggatc accaggtgcc ggccatcgtc ggacaggagc ttgggcggca
    31381 tggccttgag ggtgccgcca tggaacaggt tcaggaagaa cgtcgcgagg atgtcgtcgc
    31441 ggtggtggcc gagcgcgatc ttcgtgaagc cctcgcgttc ggcgaaggca tacagcgcac
    31501 cgcgccggag ccgtgaacac aggctgcaca tcgtcctgcc ttccggcacc acccgcgtca
    31561 ccacgctgta ggtgtcctgc tcgatcacat ggaagggcac gccgcgttcg cggcaatagt
    31621 ccgggatcac gtgtccggga aatccgggct gcttctggtc caggttcacc gcggccagtt
    31681 cgaaccgcac cggcgccttg gccttgagcg agagcagcag gtccagcagg gtccaggaat
    31741 ccttgccgcc cgacaggcat accatcacgc ggtcgccgtc ctcgacgagg ttgtagtcgg
    31801 cgatcgcgcg gccgacggcg tggcgcaggc gcttggccag gcgcgcggcc tcgtgggcct
    31861 gcttgcggcc gggaaccgat tggacatggg tggcggacat gagccggcca gtgtagcggc
    31921 gcgggcgccg gcgctacact cgcgccatgc aacccggcaa cgatcccgcc acgctgatcc
    31981 gccgtctcgc ggagctgcgc gaggagcacc gcgacctgga cgcggcgatc gcgaagctgg
    32041 tgggcgatcc cagctgcgac cagttgcagt tgagccggct caagaagcgc aagctgaagc
    32101 tcaaggacat gatcgcgtac ttcgaggaca agctgattcc ggacctcgat gcgtagggca
    32161 ggccgtgccg cttgcccggg acccggcgga agtgtccgcc cggacccgtc cggcgagccg
    32221 tcatgacatg accgaactga ttccaaccgt cctccaggat ggccatgtcg cgctggaacc
    32281 gctttcactc gaccacgtcg cgggcctgga agccgccgcc gcggacggcg agctctggaa
    32341 cctccgggtg acctacgtac ctccaccgga tgcgatgcaa gcctacgtcg agacggcgct
    32401 ggcgatgcag gctgcgggcg actcgctgcc gttcgcggtg cgcgacaagc gcgatggcgc
    32461 gctcgtgggc agcacccgct attatggctt cgacgcggaa accccgcgcc tcctgatcgg
    32521 ccatacgtgg tacgcgcgca gccgccagcg cagtccggtg aataccgcct gcaaactgct
    32581 gctgatcggc tacgccttcg agcggcttgg ctgcgccgcg gtcg
//
LOCUS       DASSNH010000238         5440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_274755, whole genome shotgun sequence.
ACCESSION   DASSNH010000238 DASSNH010000000
VERSION     DASSNH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274755"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFK00_08710"
     CDS             complement(<1..125)
                     /locus_tag="VFK00_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET7593069.1"
                     /translation="MSDRQSGTVKWFNDAKGFGFITPESGDDLFVHFRAIQGDGF"
     gene            complement(521..1462)
                     /locus_tag="VFK00_08715"
     CDS             complement(521..1462)
                     /locus_tag="VFK00_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7593070.1"
                     /translation="MLRLPNLLPFLVLALTCGAASAAETKPFFTLHEVGKGVWAAIAV
                     SGSGAGSNSGFIVGDDGVLVVDSFEDADAAKALLNVIHEKTSLPVRYVVDTHYHLDHV
                     AGNGVYQAAGAVVMAQANVRAWERTENLKFFGDEITPAQRKMVRSYVLPGVLYQDGID
                     VYLGSRKVVVRVLPGHTGGDSVVVVPDADVVFTGDLFWDHSLPNLIDANTLAQIHTNG
                     VFLTDYPTATFVPGHGDIGKASDVRAFRGYLVALRQAITTAEHKGESGTALHQDVLHQ
                     LQGTYGTWNYFDHFAPRNITQTIAELAGKKPLPEPVP"
     gene            1759..2496
                     /locus_tag="VFK00_08720"
     CDS             1759..2496
                     /locus_tag="VFK00_08720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7593071.1"
                     /translation="MMKHEEVTVGTRDGQCPVHVFTRGGEGPWPAVIVYMDAFGIRPA
                     LMQIGERLAGGGYVVLVPDLFYRYGAYGPLDPKEAFAGDFRGMVGPLMATTDNARAAQ
                     DTEALLGHLDTRDDFTGKVGTVGFCMGGGMAITAAGTYPERIAAAISFHGGRLATDDP
                     ASPHLLAPKIRAELYIAVADNDRSCPPEMVERFTKALDDAGVRYRCETYRGASHGWMK
                     PDFPVYDEAAAERGWSEMLALFGRALR"
     gene            complement(2551..3108)
                     /locus_tag="VFK00_08725"
     CDS             complement(2551..3108)
                     /locus_tag="VFK00_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET7593072.1"
                     /translation="MARVNAFFAVASIALLAACATARQQAPAVPTSSDYAARAHATLQ
                     SASGSDVAGTLSFVNTGEGVRITGRITGLEPDSTHGFHIHENGDCGAPDASSAGGHFN
                     PTHQPHGHAGQAASHAGDLPNQQANADGVATVDVLAHGVELGTGGSTDILDRAIIVHA
                     KPDDYTSQPSGDAGSRIACGVITRD"
     gene            complement(3203..5002)
                     /locus_tag="VFK00_08730"
     CDS             complement(3203..5002)
                     /locus_tag="VFK00_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7593073.1"
                     /translation="MEFQQIVFLVILAAALVLLVTEWVRIDLTGVLIVVALAGTGLLK
                     PADALSGFSSDPAILVVSMFVLSAGLAQTGVTGLLGAWIGRMAGESIWRAVLVIMPCV
                     ALMAAFSHHLMVTAMMLPVILELNRSHHLPASRLMIPMAFAASLGTTVTVIAAPAFLV
                     ARDLLERGGVPDVGIFSIAPMGIVLTLIGTAYILLFGRWLLPSHEGSGSGEDRFRLER
                     YYTELVVLESSPHVGKSMGDFGKEHAERFRVVDWLRQGSSRPRPWRRKLLASGDLLLV
                     RASPDELAALKEQQGLALSAVVQYGEEIGDDRRAAREDDERLVQAIVGPDSGLVGRTI
                     GNVDFLQRYGVVVVGLWRKRGFIRKELSKVKLEGGDLMVLWGDKRALDGLARDPSFLL
                     LVPFSGRARVRGRGWLAALIMGLSVGAAASGLLPVAISFLAGATAMVVTRCLTLRQAY
                     ESVELRIFVFIAGAIPLGLAMEQTGTATLFADWLSVLVSGWSPSWVLLALFLTAALFT
                     QVLSDTATTILLGPVALGLASLLDMSAFAMVFSVAMGAVAAFLTPIGHHGNLLVYEPG
                     HYRFMDFVRVGTPLTLLMGVAVAWLAPLLWPAG"
     gene            5250..>5440
                     /locus_tag="VFK00_08735"
     CDS             5250..>5440
                     /locus_tag="VFK00_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593074.1"
                     /translation="MKRHLLLLVLACLTCLAMPAFAARDHAALVKSVTGNVQIARHDH
                     AFKAASGTTLLVSDRVVTAS"
BASE COUNT          923 a         1788 c         1857 g          872 t
ORIGIN      
        1 ttgaaaccgt cgccctggat ggcgcggaag tggacgaaca agtcgtcacc actttccggg
       61 gtgatgaagc cgaagccctt ggcatcgttg aaccatttga ccgtgccgct ctgtcgatcc
      121 gacattacac ataacctttg agaactgtgg atgaattacg gcatcgagtg gggcgaactc
      181 gccgcaaggt gtgcagtgca tccgaagttg ccccaagcgc tgcccggcaa gcataaccga
      241 taatcgggtc gcgcgcgcgt gccggctggg ggacggggta cgggggcgcg ggagcattgc
      301 gacgccgccg acccgccccg ttgggcttcg ctgcgctcag cgccaaccta cgaaaagccg
      361 atgcgggttg gcgctggcgc cgggcttcat tgggcggaag ccccacgaag gcggaattcg
      421 cacgcaagcg gcacgtcctt ggtttttgcg ttgttgggct tcgctgcgcc cagcgccaac
      481 ctacaaaagc cgacgcaggt tggcaaagcc aggccggttg ctagggcacg ggttccggca
      541 ggggcttttt gccggcgagt tccgcgatcg tctgcgtgat gttgcgtggc gcgaaatggt
      601 cgaagtagtt ccaggtgccg taggtgcctt gcagctggtg cagcacatcc tggtgcaacg
      661 ccgttccgga ttcgcccttg tgctcggctg tcgtgatcgc ctgccgcagt gccacgaggt
      721 agccgcggaa tgcgcggacg tcggaggcct tgccgatatc gccgtggccc ggcacgaagg
      781 tggcggtcgg gtagtcggtg aggaacacac cgttggtgtg gatctgcgcg agggtgttcg
      841 cgtcgatcag gttcggcagg ctgtggtccc aaaagaggtc gccggtgaac accacgtctg
      901 cgtcgggtac gaccacgacc gaatcgccgc cggtatgtcc aggcaacacc cgcaccacga
      961 ccttgcggct gccgaggtac acgtcgatgc cgtcctggta gaggacgcct ggcagcacgt
     1021 aggaccgcac catctttcgc tgtgccggcg tgatctcgtc cccgaagaac ttgaggtttt
     1081 cggtccgttc ccacgcgcgc acgttcgctt gcgccatgac cacggcaccc gcggcctggt
     1141 agacgccatt cccggcgacg tgatcgaggt ggtagtgggt gtcgacgacg taacgcaccg
     1201 gcaaggacgt cttctcgtgg atgacgttca acagcgcctt cgccgcgtcg gcgtcctcga
     1261 agctgtccac caccagcacg ccgtcatcgc cgacgatgaa gccggagttg ctgccggcgc
     1321 cgctgccgga gaccgcgata gcggcccaga cacccttgcc gacctcgtgc agggtaaaga
     1381 agggcttcgt ttctgccgcg ctggcggcgc cacaggtcag cgccaggact aggaacggaa
     1441 gcaggttggg caatcgcagc atggcatcac ctcggcgtga tcgaacggaa ggcacccttc
     1501 gtcgggcttc ccccgggtca agcccggggt cagcgccaat ctacaaaagc aggccggccg
     1561 cgttgcagga atgcggcttc gccgcggatt ctcgtggcat tgcgcaccac gtgccacatc
     1621 atgccgcgcg ggccgcgatc ccggcccgcg ccgggacggc gatcaggggc gggcatttgc
     1681 caggcgctat catggcccgg atgcgtgcgt ggcgttcgtc gcggcgacac cacatctttc
     1741 gtggcaacgg gagcacgcat gatgaagcac gaggaggtca ccgtaggcac gcgcgatggg
     1801 caatgcccgg tgcatgtctt cacgcgcggc ggtgaaggtc cgtggccggc ggtgatcgtg
     1861 tacatggacg cctttggcat ccgcccggct ttgatgcaaa tcggcgaacg cctcgccggc
     1921 ggcggctacg tcgtgttggt gccggacctc ttctatcgct acggcgcgta cggtccgctg
     1981 gatccgaagg aagctttcgc cggcgacttc cgcggcatgg tcgggccgct catggcgacc
     2041 accgacaatg ccagggccgc gcaggacacc gaggcgctgc tgggccacct cgacacgcgc
     2101 gacgacttca ccggcaaggt cgggacggtc ggtttctgca tgggcggggg catggctatc
     2161 acggcggcgg gcacgtatcc ggagcgcatc gcggccgcga tcagcttcca tggcgggcgc
     2221 ctggccacgg acgatccggc gagtccgcac ctgctggcgc cgaagatccg cgccgagttg
     2281 tacatcgccg ttgccgacaa cgaccgcagt tgtccgccgg aaatggtcga gcgtttcacg
     2341 aaggcgctgg acgacgcggg cgtgcgttac cgctgcgaaa cctatcgcgg cgcctcgcac
     2401 ggctggatga agccggattt tccggtttac gacgaagcgg cagccgaacg cggctggtcc
     2461 gaaatgctgg cgctgttcgg ccgcgcactg cgatgaacaa gagggagacg gaggcaatca
     2521 aggcagcttt attgcctccg tcaccttttt tcagtcgcgg gtgattacgc cgcaggcgat
     2581 gcgcgagccg gcatcgccgg atggctggct ggtgtagtcg tcgggcttgg catgcacgat
     2641 gatcgcgcgg tccaggatgt cggtgctgcc gccggtgccg agttcgacgc catgggccag
     2701 cacgtccacc gtggcgacgc cgtcggcgtt cgcctgctgg ttgggcagat cgccggcgtg
     2761 gctggccgcc tgtccggcat gcccgtgcgg ctggtgcgtc ggattgaaat gcccgccggc
     2821 gctgctggca tcgggtgcac cgcagtcccc gttctcgtgg atgtggaaac cgtgggtgct
     2881 gtccggctcg agcccggtaa tgcggcccgt gatgcgcacg ccctcgccgg tgttcacgaa
     2941 cgatagcgtc ccggcgacat cgctgccgct cgccgattgc agcgtggcgt gggcgcgcgc
     3001 cgcgtaatcg gacgacgtcg gcactgccgg agcctgctgc ctggcggtgg cgcaggcggc
     3061 gagcaaggca atggatgcga cggcaaaaaa tgcgtttacg cgtgccatgc gtcttccttg
     3121 tgagcgtagg atccagcagc ttagtggacc ggcattaaag cgccgtcaat atccatgggc
     3181 gcatcgtcac gcgtgccatg attcatccgg ccggccacag cagcggcgcg agccatgcca
     3241 cggcgacgcc catcagcagg gtcaggggcg tacccacgcg cacgaagtcc atgaagcggt
     3301 agtggccggg ctcgtagacc agcaggttgc cgtggtggcc gatcggggtc aggaacgcgg
     3361 ccaccgcgcc catggccacg ctgaacacca tcgcgaaggc cgacatgtcg agcaggctgg
     3421 ccaggcccag cgcgaccggg ccgagcagga tcgtcgtcgc ggtgtcggac aggacctggg
     3481 tgaagagcgc cgcggtcagg aacagcgcaa gcagtaccca gctgggcgac cagcccgaca
     3541 ccagaaccga gagccagtcc gcgaacaagg tcgcggtgcc ggtctgttcc atcgcgagcc
     3601 cgagcggaat ggcgcccgcg atgaacacga agatgcgcag ctccaccgac tcgtaggcct
     3661 gccgcaaggt caggcaacgg gtgacgacca tcgccgtggc accggccagg aacgagatcg
     3721 ccaccggcag caagcccgat gcggcggcgc ccacgctgag ccccatgatc agcgccgcca
     3781 gccagccgcg cccgcgcacc ctggcgcggc cggagaacgg caccagcagc aggaacgagg
     3841 gatcgcgcgc tagcccgtcc agcgcacgct tgtctcccca caacaccatc aggtcgccgc
     3901 cctcgagctt caccttggac agttccttgc ggatgaagcc gcgcttgcgc cacagtccga
     3961 ccacgacgac gccgtagcgc tgcaggaagt cgacgttgcc gatggtgcgc ccgaccagtc
     4021 cggagtcggg tccgacgatc gcctgtacca ggcgttcatc gtcttcccgg gcggcgcgcc
     4081 ggtcgtcccc gatttcctcg ccgtattgca ccacggcgct cagcgcgagc ccctgctgct
     4141 ccttcagcgc cgccagttca tccgggctgg cgcgcaccag cagcaggtcg cccgaagcca
     4201 gtagcttgcg ccgccagggt ctcgggcggg agcttccctg gcgcagccaa tccaccacac
     4261 ggaagcgctc ggcatgttcc ttgccgaaat cgcccatgga cttgccgacg tgcggcgaag
     4321 actccagcac cacgagttcg gtgtaatagc gctccaggcg gaagcgatcc tcgccgctgc
     4381 cgctgccctc gtggctcggc agcagccagc gtccgaacag caggatgtag gcggtgccga
     4441 tgagggtcag cacgatgccc atcggcgcga tcgagaagat gccgacgtcg ggtacgccgc
     4501 cgcgctcgag caggtcgcgt gcgaccagga aggccggtgc ggcgatcacc gtgacggtgg
     4561 tgcccagcga cgccgcaaag gccatcggaa tcatcaaccg cgaggccggc aggtggtgcg
     4621 agcggttgag ctcgaggatc accggcagca tcatcgcggt gaccatcaag tggtggctga
     4681 aggcggccat caacgccacg cacggcatga tcaccagcac ggcgcgccag atgctttctc
     4741 cggccatgcg cccgatccac gcgccgagca ggcccgtcac gcccgtctgc gccagccccg
     4801 ccgacagcac gaacatggac accacgagga tcgccgggtc gctggagaag cccgagagtg
     4861 cgtcggcggg cttcagcagg ccggtgccgg ccagcgccac cacgatcagc acgccggtga
     4921 ggtcgatgcg cacccattcg gtgaccagca ggaccagcgc cgccgccagg atcaccagga
     4981 acacgatctg ctggaattcc atgccggaac aatcgtcggg gccgtcggcg cgatggtaat
     5041 ggcggccggc ccggcgccaa ggcgtgcccc ctcgtcgccg gacgccgcgt ggcggggccg
     5101 attcgcggca cgccgttcgt ggggcgttcg gtgagcgacc cccgcgccta ccacaacgtg
     5161 aaccttgccc catttgacgg ggttcgattg gcaccggtcg tgcaagctga tcatggggcg
     5221 gtgtatccat tccggacagg gagatcccaa tgaaacgcca tctgcttctg ctggtcctcg
     5281 cgtgcctcac atgcctggcg atgccggctt tcgcggcccg ggatcatgcc gcgttggtca
     5341 agagcgtgac cggcaatgtc cagatcgccc gccacgacca cgcgttcaag gcggccagcg
     5401 ggaccaccct gctggtttcc gaccgggtgg tgacggcgtc
//
LOCUS       DASSNH010000239         3707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1652222, whole genome shotgun sequence.
ACCESSION   DASSNH010000239 DASSNH010000000
VERSION     DASSNH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652222"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..2408)
                     /locus_tag="VFK00_08740"
     CDS             complement(207..2408)
                     /locus_tag="VFK00_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7593075.1"
                     /translation="MPPLRPAALPFALSLILFAPVAGAQEANSSNPQQTDQTTKTLST
                     INVKGEKPYDWRKYVAAKLKHQMSEVSGTEITVTKKATVTHLDLQPTVVGNNLEQLFA
                     RSPGVQVSQQPTPTQFNLSYRGLGNPQESEYVLVMQDGIPLASDWIGFPTLYAMPLSH
                     SIADIQLIRGGSSLLYGPEPAPVINLVSRRPKPGTPFSISTEQVIGSDSLYSTYNVLE
                     GSAGKWTYRADAGYVRSDGPRENAQSQMRQADLYVEYRPDETQYSWLDVHAIDADSGD
                     PGRLSFPQWQANPDVTPTPWNRDWVDRYQIVLGHQHEWANGWLLIGKLWAGYQDLASR
                     AANGPTAPDLAPQPTPTGTTLQDEQFRTQGLDVRVRRKWGQGNAFTAGVVAFHGDDPF
                     RQWDTPDLYVDRHDRTGTPRLRQQRQSNYYAVFAENVFRLPHEIHIVPSIRLEKERVA
                     VTETVRPPFLSRPLIDESDSRTVPLLGLGIGNDFGAGNETYFNVSQGWRPLRYFDMAS
                     PFSNVVPGHAPDPSKALSWEAGVHGVPLTGLYYDVSVFWIDFKNRIEAQHINATDVLE
                     VNSGDTRSRGFEGQINYDFFAPTARSAAGEHLEGFLSLSLLNARFTQSVIPDQVGKVP
                     AYAPRYVAKAGITWRQQQHFKASLTAVSTAEQYWQDSNQPFGSGATFIPAKIPAHTVV
                     DFASDWQVTPWLKLLAGVSNLTDRQYYNRVWRTGLEPAYGRTWYAGFELKM"
     gene            complement(2521..3513)
                     /locus_tag="VFK00_08745"
     CDS             complement(2521..3513)
                     /locus_tag="VFK00_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7593076.1"
                     /translation="MAAKSSTKLVVYAALIGNLLVAITKTAAAMLTGSSAMMSEAIHS
                     FVDTGNEVLLLYGMHRSARRPDPEHPLGYGRELYFWSFIVALLVFSLGAGVSIYEGIL
                     HIRKPEPITDPLINYVVLALALAFEGTSWVFSVRQFVAAKGSLGFYEAFRRSKDPTSF
                     TVFFEDTAALLGIAIAAAGTFVATSLDIEVADGVASILIGAVLAATAALLARESKSLL
                     IGERADRRISESILRVTREVDPALQTNGLFTVQLAPEQIVAVLSLEFADEARAPEIEE
                     LVTRIERKVREAHPEVVALFVKPQTRKGYESDLGGNMGDHGPLPAHASPTTPQR"
BASE COUNT          601 a         1153 c         1261 g          692 t
ORIGIN      
        1 tttacgtcca tcccgtcgtg ctcggccacg gcaagccgtt cttcgcgggc ccccgaccgc
       61 ggctccgcct ggtcgccagc gatttgatcg tcgaggacgt gatcaggctg acgtacgttc
      121 ctgcgtgatg acgccgtggg catcgacggg attgaaagca atggcgacgc gtgcggtggc
      181 ctggcgtgcc caggttctcc gctctatcac atcttcagtt cgaaccccgc ataccacgtg
      241 cggccgtagg caggctcgag gccggtgcgc cacacccggt tgtagtactg gcggtcggtg
      301 aggttgctga cgccggccag cagtttcagc catggcgtga cctgccagtc gctcgcgaaa
      361 tcgaccacag tgtgcgcggg aatcttcgct ggaatgaagg ttgcgccgct gccgaacggc
      421 tggttggaat cctgccagta ctgctcggcg gtcgacaccg cggtcaggct ggccttgaag
      481 tgttgctgct ggcgccaggt gatgccggcc ttggcgacgt agcgcggcgc gtacgcgggg
      541 accttgccga cctggtccgg gatcacgctt tgcgtgaagc gcgcgttcaa caggctgagg
      601 ctgaggaagc cttccaggtg ttcgccggcc gccgagcgcg cggtcggcgc gaagaagtcg
      661 taattgatct ggccttcgaa accgcggctg cgggtgtcgc cgctgttgac ctcgagcacg
      721 tcggtggcgt tgatgtgctg cgcctcgatg cggttcttga agtcgatcca gaacacgctg
      781 acgtcgtagt acaggccggt cagcggcacg ccgtgcacgc cggcttccca agacagcgcc
      841 ttggacggat cgggcgcgtg gccgggcacc acgttcgaga acggcgaggc catgtcgaag
      901 tagcgcagcg gccgccaccc ctgcgacacg ttgaaatagg tttcgttgcc ggcgccgaaa
      961 tcgttgccga tgccgaggcc gagcagcggc acggtgcgcg aatcggattc gtcgatcagc
     1021 gggcgcgaca ggaatggcgg ccgtacggtt tcggtgaccg cgacgcgttc cttctccagc
     1081 cggatcgacg gcacgatgtg gatttcatgg ggcaaccgga acacgttttc cgcgaacacg
     1141 gcgtagtaat tcgactgccg ctgctggcgc agccgcggcg tgccggtgcg gtcgtggcga
     1201 tcgacgtaca ggtcgggagt gtcccattgc cggaacggat cgtcgccgtg gaacgcgacc
     1261 acgcccgcgg tgaaggcgtt gccctggccc cacttcctgc gcacgcgcac gtccagcccc
     1321 tgcgtgcgga actgctcgtc ctgcagcgtg gtgccggtcg gtgtcggttg cggcgcgagg
     1381 tcgggcgcgg tcgggccgtt ggccgcgcgg ctggcgagat cctggtaacc cgcccacagc
     1441 ttgccgatca gcagccagcc gttcgcccat tcgtgctggt ggcccagcac gatttggtag
     1501 cgatccaccc aatcgcggtt ccatggcgtc ggcgtaacgt cgggattcgc ctgccattgc
     1561 gggaaactca ggcggcccgg atcgcccgaa tcggcgtcga tcgcgtgcac gtcgagccat
     1621 gaatattgcg tttcgtcagg ccggtactcg acgtacaggt cggcctggcg catctgcgac
     1681 tgcgcgttct cgcgtgggcc atcgctgcgg acgtaaccgg cgtcggcgcg gtacgtccat
     1741 ttgccggcgc tgccttcgag cacgttgtag gtggaataca ggctgtcgct gccgatcact
     1801 tgttcggtgc tgatcgaaaa cggcgtgccg ggtttgggac gcctggacac gaggttgatc
     1861 accggcgcgg gctccggacc gtacagcagg ctcgaaccgc cccggatcaa ctggatgtcg
     1921 gcgatgctgt gcgaaagcgg catcgcgtac agcgtcggaa agccgatcca gtccgatgcc
     1981 agcggaatgc catcctgcat caccagcacg tattccgatt cctgcggatt gccgagaccc
     2041 cggtaactca ggttgaactg cgtcggcgtc ggctgctgcg acacctgcac gcccggcgag
     2101 cgcgcgaaca gttgctcgag gttgttgcca accaccgtgg gctgcaggtc gaggtgcgtg
     2161 accgtggcct tcttggtcac ggtgatctcg gtgcccgaca cctcggacat ctggtgcttc
     2221 agcttggccg cgacgtactt gcgccagtcg tagggcttct cgcccttgac gttgatcgtc
     2281 gaaagggtct tcgtggtctg gtcggtttgc tgcgggttcg atgaattggc ttcctgtgcg
     2341 ccggcgaccg gtgcaaacag gatcagcgac aaggcgaagg gcagggcggc gggacgcaac
     2401 ggcggcatca gggttccatg aatgcaaacg gcaatcgttc acttttcacg atccgccgcg
     2461 tttacgccat aggccattcg tttactgctc gatcgagccc cggcatcgcg cgcctcgacc
     2521 ttacctttgg ggcgtcgtcg ggctggcgtg ggcgggaagg gggccgtggt cgcccatgtt
     2581 tccgccgaga tccgactcgt agcccttgcg cgtttgcggc ttgacgaaca gtgcgaccac
     2641 ctcggggtgc gcctccctga ctttgcgttc gatccgggtg accagttcct cgatttcggg
     2701 tgcgcgtgcc tcgtccgcga attcgagact gagcaccgcg acgatctgct ccggtgcgag
     2761 ctggaccgtg aacaggccgt tggtttgcaa tgcggggtcc acttcccggg tcacgcggag
     2821 aatcgattcg ctgatccggc gatcggcacg ctcgccgatc agcaagctct tgctttcgcg
     2881 cgcgagcagt gcggcggtcg ccgcgaggac cgcgccgatg agaatcgagg cgacgccgtc
     2941 ggcaacctcg atatcgaggg acgtagccac gaaggtgccg gcggccgcga tggcgatgcc
     3001 gaggagggcc gcggtgtcct cgaagaacac cgtgaaggat gtcggatcct tgctccggcg
     3061 aaacgcctcg tagaagccca gcgatccctt ggccgccacg aactgccgaa ccgaaaacac
     3121 ccaggaggtc ccttcgaacg ccagcgccag cgccagcacc acgtagttga tcagcgggtc
     3181 ggtgatcggc tcgggcttgc ggatgtggag gatgccctcg tagatcgaca cgccggcgcc
     3241 gagcgaaaac accagcagcg ccacgatgaa actccagaaa tacagttccc tgccatagcc
     3301 gagcggatgc tcgggatcgg gacgccgcgc cgagcgatgc atgccgtaca gcagcaggac
     3361 ttcgttgccg gtgtcgacga acgagtggat ggcctcgctc atcatcgccg agctgcccgt
     3421 cagcatcgcg gcggcggttt tggtgatggc caccagcagg ttgccgatca gggcggcata
     3481 gacgacgagt ttggtcgatg atttggctgc cacgatgcct gccgcaatgg ggcgcagcaa
     3541 ccaccatacg ggcgagccgg gagcgtcgcg tgaaagacgg gtgaatgctt ccaacgcgcc
     3601 gcgcggggcg actgcgcgct cgttgggctt cgctgcgctc agcaccaacc tacaggagca
     3661 ctccggcctc accttggtag gttgggttga tcccggaact tgttccg
//
LOCUS       DASSNH010000240        14867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1413684, whole genome shotgun sequence.
ACCESSION   DASSNH010000240 DASSNH010000000
VERSION     DASSNH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413684"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..3618)
                     /gene="smc"
                     /locus_tag="VFK00_08750"
     CDS             complement(112..3618)
                     /gene="smc"
                     /locus_tag="VFK00_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7593077.1"
                     /translation="MRLSTIKLAGFKSFVDPTILHLPTNLTGICGPNGCGKSNIIDAI
                     RWVMGESAASRLRGEAITDVIFSGSSGRKPVGQATVELIFDNSDGTLGGEYAGFNEIS
                     VKRSVSRDSQSDYYLNGVRCRRRDITDLFLGTGLGARSYSIIEQGVISDIVESRPEQL
                     RIHLEEAAGVSRYKERRKETESRIKATRENLDRVKDVREEVEKQLDHLNRQARAAERW
                     KKLKEERGLREAELRALNYRSVTAQLETHRQALREAETALEGKLAAQRHLEAEIETGR
                     EAHQQHTEAMNAVQAEVYQVGAELARVEQQIRHHREMLERLQHDRAESERAWTELADH
                     IKADTSQHDELKAAVAEAEPETGRLREAEARAAQALNDAEAAHAAWQQRWEAHSGQTA
                     STSREAEVERTHIDHLDKQALDLSRRREALRNERKNYDLDALTAGTAGLADEHALLKT
                     QVDAHAQGLDGHKADAERLLGEERQAQTWLAEANAQKQDLRGRIASLEALQRAALGRD
                     RDAAREWLQKVGLADGERLGARLKVNGGWERAVETVLAGMLEAVLVDDPAVHAGELAE
                     VAKSDITLIGSRAALDLDVAGTLAAEVDGPAAARALLAKVRTAGSIDEARNIASTLAP
                     TDTVITRDGVWLGPGFARVRRAEGGQVGVLARERELQEATTELAATEKRIADLQQRQA
                     EIRDQRRTAEQARDDAQRDLYNAHRRLSELAGEMQSRQGRIDSARARLEAIDTEHAEV
                     EKALAENEQLLQAARARMQSAVANMGEHEQARVALESERRHLLERREAARADAQEASD
                     AAHRHAIALESRRVTLAALEQSLARMRTQHEQLQRHRADLDRRLAEGSDPTAVLDNER
                     QVCLEQRLSVDKKLVEARRVVEESANSLREREQARHAAEQTMGDERDALEKLRLEEQG
                     HRLRAEQLAEAITEAGLEIEPLLANLSDEVQPEAWQGKIADLEARIARLEPVNLAAIT
                     EHAEAAQRKEYLDAQMTDLTTALDTLENAIRKIDRETRERFKDTFDRVNAGFQDLFPK
                     LFGGGHAYLELTGEDLLDAGVTMMARPPGKRNSHISLLSGGEKAMTAVALVMAIFNLN
                     PAPFCLLDEVDAPLDEGNIGRFSQLVRDMSEKVQFMVVTHNKATMEMAYQLCGVTMRE
                     PGVSRLVQVDLAEAAKLAGLAA"
     gene            complement(3835..4092)
                     /locus_tag="VFK00_08755"
     CDS             complement(3835..4092)
                     /locus_tag="VFK00_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET7593078.1"
                     /translation="MSAAPVEGIRARLERAFAPTSLEVIDEGHLHIGHAGEGSGHFRV
                     RIASAAFEGKPRVQQHRMVYDALADLMGHGIHALAIEARAP"
     gene            complement(4089..4388)
                     /locus_tag="VFK00_08760"
     CDS             complement(4089..4388)
                     /locus_tag="VFK00_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7593079.1"
                     /translation="MWYAITGIDIPNSLDKRKGARPVHLARLQKLLEEGRLLLAGPFP
                     AIDAEDPGPAGFSGTLIIAEFDDLEAARQWADADPYRVAGVFREVTVRPFRQTLP"
     gene            complement(4528..6045)
                     /gene="lysS"
                     /locus_tag="VFK00_08765"
     CDS             complement(4528..6045)
                     /gene="lysS"
                     /locus_tag="VFK00_08765"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7593080.1"
                     /translation="MSETNDQVATPAADENKLIAERRDKLRALRGQGIAFPNDFRPDA
                     FAGELQRETEGADSEALNAKARRVRIGGRLLGKRIMGKASFARVQDMTGSIQLFLQKD
                     ALAETYDAFKGWDVGDIVGAEGTLMRTRTGELSVKVDALRLLTKSLRPLPDKWHGLAD
                     VEQRYRQRYVDLIVTPEARETFKKRSAIVGFMRQWLEAEPRRFMEVETPMMHVIPGGA
                     TARPFVTHHNALDIDLYLRVAPELYLKRLVVGGFERVYEINRNFRNEGVSTRHNPEFT
                     MLELYQAYASYNEIMDLTEAMIRETAKAVLGTTDLRWEERDIDVGKPFRRWSLSDAVR
                     ELNPGIAEHELRDPAALRAHCARLKVPVKNEYGWGKLLLEIFEKTVEHTLVQPTFITQ
                     YPTEVSPLARANDADPEITDRFELFIGGKEMANGFSELNDPEDQAARFRAQVEALAHG
                     DDEAMHFDADYIRALEVGLPPTGGLGVGIDRLVMLFTDSASIRDVLLFPYMRPEG"
     gene            complement(6150..7275)
                     /gene="prfB"
                     /locus_tag="VFK00_08770"
     CDS             complement(join(6150..7199,7201..7275))
                     /gene="prfB"
                     /locus_tag="VFK00_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403117.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7593081.1"
                     /translation="MIESNPIRAHIEDLRERVESLRGYLDYPARRERLEEVTRELESP
                     NVWDDPQRAQDLGRERARLAEFVEGLDQITHGLDDAGELLELSADEGDDASLQSVSND
                     VDALEKRVAQLEFHRMFAGEMDPSNAFVDIQAGAGGTEAQDWAEMLLRMYLRWAEDRG
                     WQTELLEASAGEVAGVKSATFRVAGDYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFA
                     SVFVSPEVDDDIDIEINPADLKTDVYRSSGAGGQHVNKTESAVRITHVPSGIVVACQT
                     ERSQHANRDRAMKMLRAKLYEMELQKRNAEKAAVEATKSDVGWGNQIRNYVLDQSRIK
                     DLRTGVERTDTQRVLDGDLDEFIEASLKMGLEAGAKRADA"
     gene            7418..7831
                     /locus_tag="VFK00_08775"
     CDS             7418..7831
                     /locus_tag="VFK00_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593082.1"
                     /translation="MTPEPASDRHLRWRIFPAVAIIAIGVLFLLNNLGYNLAFLDRGN
                     WWAVIILLAAFAPLTRAWETYRERGRADAEVAHSLLCGIAVALVGVMFLLELDWGTWW
                     PLFVILGGLFALAPHRSRGERHRYGGSEDDASIKR"
     gene            complement(7821..8381)
                     /locus_tag="VFK00_08780"
     CDS             complement(7821..8381)
                     /locus_tag="VFK00_08780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET7593083.1"
                     /translation="MSKGQPVIAVLVGSLRKGSFNKQLAHALEKIAGDRAGFRHIRID
                     DLPLYNQDFDGDYPKVCEDRKQEIRSCDGVLFVTPEYNRSVPGVLKNAIDVCSRPYGT
                     NAFDGKPGAVIGTSAGAMSTALAQQHLRNICVFLDIPMLQQPEAFVRYTEGLFGRDGS
                     VTDDSARRFLGNFVDKYLDWVKRFSA"
     gene            complement(8418..9245)
                     /locus_tag="VFK00_08785"
     CDS             complement(8418..9245)
                     /locus_tag="VFK00_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593084.1"
                     /translation="MVAMIRRKLALFGCAALLAGSAFAAGPITASGDTPAMLQEAVAG
                     AWRSPANKARDQYRHPVATLEFFGIKPDMTVIELSPGGGWYTEILAPFLYAHGHLIEA
                     APPSRDGGPSRFEQMLKGNPEIYGHVAKVVPFSPPDHVNLGPEGSADMVLTFRNTHDW
                     LNRSPETLDAVFQAAYKVLKHGGIFGVTDHRARPFADAAESSKTLHRIPEDYMVEAGL
                     RAGFRLAGVSDINANPKDPEDINVHHLPPDLAGPDSEHAKMQAIGESDRMTLKFVKP"
     gene            9337..9996
                     /locus_tag="VFK00_08790"
     CDS             9337..9996
                     /locus_tag="VFK00_08790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7593085.1"
                     /translation="MSRRTLNLDDTLYQYLLDHSLREHPEQTALRQATAGHPRARMQI
                     SPEQGQFMQLLVKLIGARRTIEVGTFTGYSALTVALALPADGKVLACDISDEYTSIGK
                     SYWQRAGVAGRIELVIAPALQTLDARIAAGEAGTYDFAFIDADKTGYDAYYERCLQLV
                     RAGGLIAIDNSLWGGDVAKPAQDDDTRALQALNEKLHGDERIDMALLPIGDGLSLARK
                     R"
     gene            complement(10140..11849)
                     /gene="recJ"
                     /locus_tag="VFK00_08795"
     CDS             complement(10140..11849)
                     /gene="recJ"
                     /locus_tag="VFK00_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7593086.1"
                     /translation="MSTGAARLRIVRRTAGGAPRDWLASVHPVVQRVYAARGVCCPDD
                     AGHRLAGLLPPTGLAGLDRALELLTDAIANDRSILVAADYDCDGATGCAVAVRGLRML
                     GARYVGYVVPDRARHGYGLTPALVDTMQPAPDLIVTVDNGIASLAGVAAAKARGCRVI
                     VTDHHLPGPRLPEADAIVNPNLPGDGFASKYLCGAGVVFYLLLALRAAMGKRGDVDLA
                     SLLDLVALGTVADLVALDRNNRALVEGGLRRIRAGRACAGVAALFEISGRDAGTAVAA
                     DLGFAVGPRINAAGRLEDMSIGIECLLSNDPSHARALAAALHAINAQRRDLQTAMVDD
                     ALARLAGLAAGDALGIALFDQSWHPGIVGLVAARVKERLHRPVVAFAPAGEGDSLRGS
                     ARSVPGFHVRDALAEVDARCPGLIERFGGHAMAAGLSLALSNYVRFAELFDAVVRDRI
                     LEESLQPVLLSDGELAPAELELELARQLRLAGPWGQGFAAPLFDNVFECAAQKSMGAD
                     GAHRRLRLRDPRDGRLLDGVRFHADGDIPQHVPLRIAFELMIDEWQGRESMRLLLRHW
                     ELA"
     gene            complement(11846..12754)
                     /locus_tag="VFK00_08800"
     CDS             complement(11846..12754)
                     /locus_tag="VFK00_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET7593087.1"
                     /translation="MSVLHVLLPSPPPCSDAALRRWLARGDRLPDVPGSRDAQVRRLF
                     RFPGEAFPVAALRHHVHAGDAARGSWLCADPAYVRGEPTGARLMACPIPDLSGDEAQA
                     LAATLGPLFEDAGLALAADSPSAWCLHVGQALAGVAFTRPADALGADLLACLPAGEGG
                     RRWRHLFSGAQIALHAHPVNAARIAAGKVPVNALWFWGAGSLPVAVETDLTVVATTDD
                     VVRGLAKLAGVDIAEPSPEALESTVGDGLLDLGAVMSSEESSRWLPFFEQALGRRRFD
                     SVELVLPAGERFRVRHAYRLRFWRRG"
     gene            complement(12751..14727)
                     /locus_tag="VFK00_08805"
     CDS             complement(12751..14727)
                     /locus_tag="VFK00_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7593088.1"
                     /translation="MIGFRDFALRRGGRLLLAGVDAVLHDGWKIGVAGRNGTGKSSLF
                     AAILGEVEADAGALDIPAGLRIASVAQETPALSEAAIDYVLAGDTELAAALADEARAQ
                     AAGDHAAAAAAHHRIDAMGGYDGRARAGRLLHGLGFKAGEHERAVAEFSGGWRGRLNL
                     ARALMAPSDLLLLDEPTNHLDLDAVLWLEQWLRRYPRTLMVISHDRDFLDGTVDHVLH
                     LEQGGARLYAGNYSDFVRQRAERLHQQQAAFAKEQAERAHLQSFIDRFRAKATKARQA
                     QSRIKRLEKLAGTEAVRAERTFEFRFPEPARVPQSMLKLEHVDAGYGLAPDVGAAQGR
                     DHATADEREGPRPWAAPTSEIARILSGVRFNVEAGDRIGLLGPNGAGKSTLVKTLVGE
                     LPPLAGMREAHKDLVIGYFAQHTVESLRAGETPFDHLRELAPDAPAQVLRDWLGQWHF
                     AGDRAFDPVDGFSGGERARLALALIAWRKPNLLVLDEPTNHLDLDMREALADALNDYP
                     GALVLVAHDRHLLGLVCDGFWRVADGAVAPFDGDLDDYARWLRTRNSEPVARETTASP
                     AASPRERRREAAAMRERQRAQRKHLEKIEARMAAIDTVLAQLQERLADPAICEGPTAV
                     LADIGREQNALREEKTALENEWLARYEALEAATA"
     gene            complement(14724..>14867)
                     /locus_tag="VFK00_08810"
     CDS             complement(14724..>14867)
                     /locus_tag="VFK00_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004085672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET7593089.1"
                     /translation="EADIKKGLIAVSSPIARAMIGKREGDSFAFEAPAGERNYEITGV
                     RYV"
BASE COUNT         2265 a         5117 c         4905 g         2580 t
ORIGIN      
        1 cgctgagcgc agcgaagccc aacgatcttc gatccatgtc gcggctgttg ggcttctccc
       61 ggaccttgtc cgggatcaac ccaacctaca aaacgcagca aagccctacg attaagcggc
      121 gaggcctgcg agcttcgcgg cctcggccag gtcgacctgc accaggcgcg atacgccggg
      181 ttcgcgcatg gtcacgccgc acaactggta ggccatttcc atggtcgcct tgttgtgggt
      241 cacgaccatg aactggacct tttcggacat gtcgcgcacg agctggctga agcggccgat
      301 gttgccctcg tccagcggcg cgtccacctc gtcgagcaga cagaacgggg cggggttcag
      361 gttgaagatc gccattacga gcgccaccgc ggtcatcgcc ttttcgccgc ccgacaacag
      421 cgagatgtgg ctgttccgct tgccgggcgg ccgggccatc atcgtcacgc cggcatcgag
      481 caggtcttcg ccggtcagtt ccagataagc gtggccgccg ccgaacagtt tcgggaacag
      541 gtcctggaag ccggcattga cgcgatcgaa agtgtccttg aaacgttcgc gggtttcccg
      601 gtcgattttg cggatcgcgt tttcgagcgt atcgagggcc gtggtgaggt cggtcatctg
      661 cgcgtcgagg tattccttgc gttgcgcggc ttcggcgtgc tcggtgatcg cggcgaggtt
      721 gaccggttcc agccgcgcga tgcgcgcttc gaggtcggcg atcttcccct gccaggcctc
      781 gggctgtacc tcgtcggaaa gattcgcgag caacggctcg atttccagtc cagcctcggt
      841 gatcgcttcg gccagttgtt cggcgcgcag ccggtggccc tgctcttcca ggcgcagctt
      901 ttccagtgcg tcgcgctcgt cgcccatggt ctgctcggcg gcgtgccgcg cctgctcgcg
      961 ttcccgcaag ctgttcgcgg attcctcgac cacccgacgg gcctcgacca gcttcttgtc
     1021 caccgagagc cgctgttcaa ggcaaacctg gcgttcgttg tccagcactg cggtcgggtc
     1081 gctgccttcg gcaagacgcc ggtccaggtc cgcgcgatgc ctttgcagtt gctcatgctg
     1141 ggtgcgcata cgggccagcg attgttccag cgccgccagt gtcacgcgcc tggattccag
     1201 cgcgatcgcg tggcgatggg cggcgtcgga ggcttcctgc gcatcggcac gcgcggcctc
     1261 gcgacgctcc agcaaatgcc gacgctccga ttccagcgcg acgcgcgcct gctcgtgctc
     1321 gcccatgttt gcgacggccg attgcatgcg cgcccgcgcg gcctgcagca actgctcgtt
     1381 ttcggccagc gccttctcga cctcggcatg ttcggtatcg atggcctcca gtcgggcacg
     1441 cgcgctgtcg atgcggcctt ggcgactctg catctcgccg gcgagttccg acaggcgccg
     1501 atgcgcgttg tacaggtcgc gctgggcatc gtcgcgcgcc tgctcggcgg tgcggcgctg
     1561 gtcccggatt tcggcctgcc gctgctggag gtcggcgatg cgcttctcgg tcgcggcgag
     1621 ttccgtggtg gcttcctgca actcccgctc gcgcgccaac acgccgacct gcccgccttc
     1681 cgcgcgccgg acgcgtgcaa agcccgggcc gagccacacc ccgtcgcgcg tgatgacggt
     1741 gtcggtgggc gcgagggtcg aggcaatgtt gcgcgcttcg tcgatcgatc cggcggtgcg
     1801 caccttcgcc agcaaggcgc gggccgcggc cgggccatcc acttcggcgg ccagcgtgcc
     1861 ggcgacgtcc aggtcgagcg ctgcacggct gccaatcagc gtgatgtcgg atttcgcgac
     1921 ctcggcgagc tcgcccgcgt ggactgcggg atcgtccacc aggacggctt ccagcatgcc
     1981 ggccagcacg gtttccaccg cgcgctccca gccgccgttg accttcagcc gcgcacccag
     2041 gcgttcgccg tcggccagcc cgaccttttg cagccattcg cgcgccgcat cacggtcgcg
     2101 gccgagcgcg gcgcgctgca gcgcttccag tgaggcgatc cgcccgcgca ggtcctgctt
     2161 ttgcgcgttg gcctcggcca gccacgtctg ggcctggcgt tcctccccca ggaggcgctc
     2221 ggcgtcggcc ttgtgtccgt ccaggccctg cgcgtgcgca tccacctggg ttttcagcaa
     2281 tgcgtgttcg tcggcgaggc ccgcggtgcc ggcggtcaac gcatccaggt cgtagttctt
     2341 gcgttcgttc cgcaaggcct cgcgccggcg cgacagatcc agcgcctgct tgtcgagatg
     2401 gtcgatgtgg gtgcgctcga cctcggcttc acgcgaggtc gaagcagtct ggccgctgtg
     2461 ggcctcccag cgctgctgcc acgcggcgtg tgcggcttcc gcatcgttca atgcctgggc
     2521 ggctcgagcc tcggcttcgc gcaggcgccc cgtctccggt tcagcctctg ccacggccgc
     2581 tttcagttca tcgtgctggc tggtatcggc cttgatgtga tcggcgagct ccgtccacgc
     2641 gcgctcggat tccgcgcgat cgtgctgcag gcgttccagc atttcccggt ggtgccggat
     2701 ctgctgctcg acccgcgcga gttccgcgcc gacctggtag acctcggcct gcacggcgtt
     2761 catcgcttcg gtgtgctgct ggtgcgcttc gcggccggtt tcgatttccg cttcgaggtg
     2821 gcgctgggcg gcgagcttgc cttccagcgc ggtttccgcc tcgcgcagcg cctgtcggtg
     2881 ggtttcgagc tgcgccgtga ccgagcggta gttcagcgcg cgcaattcgg cttcgcgcaa
     2941 gccacgttct tccttcagct tcttccagcg ctcggcggcg cgcgcctggc ggttcaaatg
     3001 gtcgagctgc ttctcgactt cctcgcgcac gtccttgacc cggtcgaggt tctcgcgggt
     3061 ggccttgatg cggctttcgg tttccttgcg gcgctccttg tagcgcgaca cgccggcggc
     3121 ttcttccagg tggatccgca actgctcggg ccgcgattcc acgatgtccg aaatcacgcc
     3181 ctgctcgatg atcgagtagc tgcgcgcgcc gaggccggtc ccgaggaaca ggtcggtgat
     3241 gtcacggcgg cggcagcgca cgccgttcaa gtagtaatcg gactgcgagt cgcggctgac
     3301 gctgcgcttc accgagatct cgttgaagcc ggcgtattcg ccacccaggg tgccgtcgga
     3361 gttgtcgaag atcagctcga ccgtcgcctg cccgaccggt ttgcgtcccg acgagcccga
     3421 aaagatcacg tcggtgatgg cctcgccgcg caggcggctg gccgcgctct cgcccatcac
     3481 ccagcggatt gcgtcgatga tgttggactt gccgcagccg ttggggccgc aaatgccggt
     3541 gaggttggtc gggaggtgga ggatggtcgg atcgacgaac gacttgaatc cggccagctt
     3601 gatggtcgac aggcgcatgg gtcaggactt ggatgtcacg gcaacggcgg gtccggcgcg
     3661 cagcgcaccg gtcgaatgcg ccactgtgcc aagggcggcg gcgggaagca acgaaaaatt
     3721 ctgcggggtc ttggcagtca atgacttgcg ggacaatctt cccggccact cccgttcggg
     3781 cgcagggggc aagcgcgcgt ccctgcacgt cctcggcatg gctcggtcgg gatcctacgg
     3841 cgcccgcgcc tcgatcgcga gcgcatggat gccgtggccc atcaggtcgg ccagcgcgtc
     3901 gtacaccatc cggtgctgct gcacccgcgg cttgccctcg aaggctgcgc tggcgatgcg
     3961 cacccggaaa tgcccgctgc cctcgccagc atgtccgata tgcaagtggc cttcgtcgat
     4021 tacctcgagc gacgtcggcg cgaacgcccg ctccagccgc gcgcggatgc cttcgaccgg
     4081 cgccgcgctc acggcagggt ctggcgaaac ggccgcaccg tcacttcccg gaacacgccg
     4141 gccacccggt agggatcggc atccgcccac tggcgcgcgg cttccaggtc gtcgaactcg
     4201 gcgatgatca gggtaccgct gaatccagcc gggccgggat cttccgcatc gatggccggg
     4261 aacggcccgg ccagcagcag gcggccttct tccaggagct tctgcaagcg agcgaggtgc
     4321 accggcctcg cacccttgcg cttgtccagg gagttgggga tgtcgatacc ggtgatggcg
     4381 taccacatgg gagattccga tcgttttggc gggttggccc tccccttcaa cgggagggtt
     4441 gggcggggat gggtatgcgc cgcgcgcgcc ccgtacccct cccggccttc cttgtgaagg
     4501 gaaggaggaa agctcgcgac gccaagctta tccttccggc cgcatgtagg gaaacagcag
     4561 gacatcacgg atcgaggcgg aatcggtgaa tagcatcacc agccggtcga tgccgacgcc
     4621 caggccgccg gtcggcggca ggccgacttc cagcgcgcgg atgtagtcgg cgtcgaaatg
     4681 catggcttca tcgtcgccat gggccagtgc ctcgacctgc gcccggaagc gcgccgcctg
     4741 gtcctcgggg tcgttcaatt ccgagaagcc attggccatt tccttgccgc cgatgaacag
     4801 ctcgaagcga tcggtgattt cggggtccgc gtcgttggcc cgggcgagcg gcgacacctc
     4861 ggtgggatac tgggtgatga aggtcggctg caccagggtg tgctcgacgg tcttctcgaa
     4921 aatctcgagg agcagcttgc cccagccgta ttcgttcttt accggcacct tgagtcgcgc
     4981 gcaatgcgcg cgcaaggcgg cgggatcgcg caattcgtgt tccgcgatcc cggggttgag
     5041 ttcgcgcacc gcgtcgctga gcgaccagcg ccggaacggc ttgccgacgt cgatgtcgcg
     5101 ttcttcccac cgcaggtcgg tggtgccgag taccgctttc gcggtctccc ggatcatggc
     5161 ctcggtcagg tccatgatct cgttgtagct ggcgtaggcc tggtagagct ccagcatcgt
     5221 gaactcgggg ttgtgccggg tggacacccc ttcgttccgg aagttgcggt tgatctcgta
     5281 cacacgctcg aaaccaccga ccaccagccg cttgaggtac agctccggcg ccacgcgcag
     5341 gtacaggtcg atgtcgagcg cgttgtggtg ggtgacgaag ggccgtgcgg tcgcgccgcc
     5401 cgggatcacg tgcatcatcg gggtctcgac ttccatgaaa cggcggggct cggcttccag
     5461 ccactggcgc atgaagccga cgatcgccga acgcttcttg aaggtttcgc gcgcctccgg
     5521 cgtcacgatc aaatcgacgt agcgctgccg gtagcgctgt tcgacgtcgg ccaagccgtg
     5581 ccacttgtcg ggcaaggggc gcagcgattt cgtcagcagg cgcagcgcgt ccaccttgac
     5641 cgacaattca ccggtcctgg tgcgcatcag cgtgccctcg gcgcccacga tgtcgccgac
     5701 gtcccagccc ttgaacgcgt cgtaggtttc ggccagcgcg tccttctgca ggaacaactg
     5761 gatcgagccg gtcatgtcct gcacgcgcgc aaagctggcc ttgcccatga tgcgcttgcc
     5821 gagcaggcgg ccgccaatcc gcacgcggcg cgctttggca ttcaatgcct cggaatcggc
     5881 gccctcggtt tcacgctgca gctcgccggc aaatgcgtcg ggtcgaaaat cgttcgggaa
     5941 cgcaatcccc tgtccgcgca acgcccgcag cttgtcgcgc ctttcggcga tcagcttgtt
     6001 ttcgtccgcc gctggcgtgg cgacctgatc gttggtttct gacatttccg ggctagctct
     6061 gttgcgtgat cttgcgcatc gtaccggacc ctcatccggc gcttcgtgcc accttctccc
     6121 gaagggagaa gggaaacctc cgaaaacctc tacgcatcgg cgcgtttggc gccagcttca
     6181 aggcccatct tcaggctcgc ttcgatgaac tcgtcgaggt cgccgtcgag gacgcgctgg
     6241 gtatcggtgc gctcgacgcc ggtgcgaagg tccttgatgc gcgactgatc cagtacgtag
     6301 ttgcggatct ggttgcccca gccgacgtcc gacttggtcg cttccacggc ggccttctcg
     6361 gcgttgcgct tctgcaattc catctcgtac agcttggccc gcagcatctt catcgcgcgg
     6421 tcgcggttgg cgtgctggga gcgttcggtc tggcaggcca ccacgatccc gctcggcacg
     6481 tgggtgatac ggacggccga ctcggtcttg ttgacgtgct ggccaccggc gccggacgaa
     6541 cggtatacat cggtcttcag gtcagccggg ttgatctcga tgtcgatgtc gtcgtcgact
     6601 tcgggcgaca cgaacaccga ggcgaaactg gtgtgccgac ggttgtcgga atcgaacggc
     6661 gatttccgca ccagccggtg cacgccgatc tcggtcttga gccagccgta ggcgtagtcg
     6721 ccggccacgc ggaacgtcgc cgacttgacg cccgcgacct cgccggccga cgcttccagc
     6781 agttccgtct gccagccacg atcttccgcc cagcgcaggt acatgcgcag cagcatctcc
     6841 gcccagtctt gggcctcggt gccaccggcg cccgcctgga tgtcgacgaa ggcgttcgac
     6901 gggtccattt cgcccgcgaa catgcggtgg aactcgagct gggcgacgcg cttttcgagc
     6961 gcgtcgacat cgttcgagac ggattgcagc gaggcgtcgt cgccttcgtc ggcggacagc
     7021 tccagcagct cgccggcgtc gtccagcccg tgcgtgatct ggtcgaggcc ttccacgaat
     7081 tcagccagcc gcgcgcgttc gcgacccagg tcctgggcac gctgcggatc gtcccagacg
     7141 ttcgggcttt cgagttcgcg ggttacttct tcgaggcgtt cgcgcctggc cgggtagtca
     7201 aagatacccc ctgagcgatt ccacgcgttc acgcaggtcc tcgatgtggg cgcggatggg
     7261 gttgctctcg atcatgtggg gtcgctcgcg aaaagaggcg aatggtagca aagccgcgcg
     7321 ggcgccgcat gcgtcagcgc cacttgctga cgactgtcac tggcgccggc aacccattgg
     7381 gcctagagtg gacccgtcct tcccgcgagg caccgccgtg acccccgaac ccgcatcgga
     7441 ccgccacctg cggtggcgca tcttccctgc cgtcgcgatc atcgcgatcg gcgtgctgtt
     7501 cctcttgaac aacctcggct acaacctcgc gttcctcgac cgggggaact ggtgggccgt
     7561 catcatcctg ctggcggcat tcgcgccact gacgcgcgcc tgggaaacct accgcgagcg
     7621 cggccgggcc gacgccgaag tcgcgcactc gctgttgtgc ggcatcgccg tcgcgctggt
     7681 cggcgtgatg ttcctgctcg agctcgactg gggcacgtgg tggccgctgt tcgtgatcct
     7741 gggcggcttg ttcgcgctgg cgccgcatcg atcccgcggc gagcgccacc gctacggcgg
     7801 cagcgaagac gacgcgtcga tcaagcgctg aagcgcttta cccagtcgag gtacttgtcg
     7861 acgaagttcc cgaggaacct cctggcgctg tcgtccgtca cgctgccgtc ccggccgaac
     7921 aggccttccg tgtaacggac gaaggcttcg ggctgctgca gcatcgggat gtcaaggaac
     7981 acgcagatat tgcgcaggtg ctgctgcgcc agcgccgtcg acatcgcgcc cgccgaagtc
     8041 ccgatcaccg cgccgggctt gccgtcgaag gcattggtgc cgtaggggcg cgagcacacg
     8101 tcgatcgcgt tcttcaacac gccaggcacc gagcggttgt actcgggcgt cacgaacagc
     8161 accccgtcgc acgaccggat ttcctgcttg cggtcctcgc acaccttcgg gtagtcgccg
     8221 tcgaaatcct ggttgtagag cggcaggtcg tcgatgcgga tgtggcggaa cccggcgcgg
     8281 tcgccggcaa tcttctccag cgcgtgcgcc agttgcttgt tgaaagaacc cttgcggaga
     8341 ctgccgacca gtaccgcgat cacgggttgt cctttgctca tggtgttact ccttgtggtg
     8401 tcgctcgagc atgcccgtca gggcttcacg aatttcagcg tcatcctgtc cgattcaccg
     8461 atcgcctgca tcttcgcatg ctctgaatcc gggccggcga ggtcgggcgg caggtggtgc
     8521 acgttgatgt cttccggatc cttcggattg gcattgatgt ccgaaacccc ggcgagccgg
     8581 aaaccggcac gcagtccggc ctcgaccatg taatcctccg gaatgcggtg cagcgtcttc
     8641 gagctttccg ccgcgtcggc gaacggcctg gcgcgatggt cggtgacgcc gaagatgccg
     8701 ccgtgcttca gcaccttgta cgccgcctgg aacaccgcat ccagggtttc ggggctgcgg
     8761 ttcaaccagt cgtgcgtgtt gcggaaggtc agcaccatgt cggccgatcc ctccggcccc
     8821 aggttcacgt gatccggcgg cgaaaacggc accaccttcg cgacgtggcc gtaaatctcg
     8881 gggttgccct tcaacatctg ctcgaaacgg gacggaccgc cgtcgcgcga tggcggcgcg
     8941 gcttcgatca gatgcccgtg cgcatagagg aacggcgcga ggatctcggt ataccagccg
     9001 ccgcccggcg acagctcgat caccgtcatg tccggcttga tcccgaagaa ttccagtgtc
     9061 gcgaccgggt gccggtactg gtcgcgcgcc ttgttggcgg gcgagcgcca ggcgcccgcg
     9121 acggcttcct gcaacatcgc aggggtgtcg ccggatgccg tgatcggtcc cgcggcgaac
     9181 gcgctgccgg caagcaaggc ggcgcacccg aacaaggcaa gctttcggcg gatcatggca
     9241 accatgtcgc gatctcctgc gggcggaatc ccaagggtac gccgaaacgg atggcgccgc
     9301 gcgataatcg ggatgcaatc ggaaccggaa gccgccatgt cccgccgcac gctcaacctc
     9361 gacgacacgc tgtaccagta cctgctcgac cattcgctgc gcgagcatcc cgaacaaacc
     9421 gcgctgcgcc aggcaacagc cgggcacccg cgcgccagga tgcagatctc gcccgagcag
     9481 ggccagttca tgcagttgct ggtgaagctg atcggcgccc ggcgcacgat cgaagtcggc
     9541 acgttcacgg gctacagcgc gctcacggtt gcgctggcgt tgcccgccga cggcaaggtc
     9601 ctcgcctgcg acatcagcga tgaatacacc tcgatcggca agtcgtattg gcagcgcgcc
     9661 ggggtcgcag gcaggatcga actcgtgatc gcgcccgctc tgcaaacact ggatgcgcgc
     9721 atcgcggcgg gggaagccgg aacgtacgac ttcgccttca tcgacgccga caagaccgga
     9781 tacgacgcct attacgaacg ctgcctgcaa ctggtccgcg cgggcggact gatcgccatc
     9841 gacaatagct tgtggggcgg cgacgtcgcc aagcccgcgc aggacgacga tacccgcgcg
     9901 ctgcaggcgc tcaacgaaaa gttgcacggc gacgaacgga tcgacatggc gctgctgccg
     9961 atcggcgacg gcctcagcct ggcgcgcaag cggtaattgt ccatccgagc gacaaatgga
    10021 taccggcctg agccggtatc acgagcaaaa tcgcttttcc aggttgggtt gatgccggac
    10081 ttgctccggg agaagcccaa caaccaccgc cgagccctgc gaaaagatct cccgagtgcc
    10141 tacgccagct cccaatgccg cagcagcagc cgcatggatt cgcggccctg ccattcgtcg
    10201 atcatcaatt cgaacgcgat gcgcaacggg acgtgctgcg gaatgtcgcc atccgcatga
    10261 aaccgcacgc cgtcgagcaa gcgaccatca cgggggtcgc gcaggcgcag gcgacggtgc
    10321 gcgccgtcgg cgcccatcga tttctgcgcc gcgcattcga acacgttgtc gaacagcggc
    10381 gcggcgaatc cctgccccca gggcccggcc aggcgcagtt gccgcgccag ttcgagttcc
    10441 agctccgcgg gcgcgagttc gccgtcgctc agcagcaccg gttgcagcga ctcctcgagg
    10501 atgcgatccc gcaccacggc gtcgaacaac tcggcgaagc gaacgtagtt cgacaacgcg
    10561 aggctgagcc ccgccgccat cgcgtggccg ccgaaccgct cgatcagccc cgggcagcgc
    10621 gcatcgactt cggccagcgc atcgcgaacg tggaatccgg gcaccgaacg cgccgaaccg
    10681 cgcagcgaat cgccctcgcc cgcgggcgcg aacgcgacca ccggccgatg cagccgttcc
    10741 ttcaccctcg ctgccaccaa cccgacgatg ccgggatgcc atgactggtc gaacaacgcg
    10801 atgccgagcg catcgccggc ggcgagcccg gcgaggcgcg ccagcgcatc gtccaccatc
    10861 gccgtctgca ggtcccggcg ctgcgcattg atggcgtgca acgccgccgc cagcgcccgc
    10921 gcgtgcgaag ggtcgttcga cagcaggcac tcgatgccga tcgacatgtc ctcgagacgc
    10981 cccgcggcat tgatccgcgg gcccaccgcg aaccccaggt cggcggcgac cgcggtgccg
    11041 gcgtcgcggc cggagatttc gaacaatgcc gcgaccccgg cgcaggcacg gccggcacgg
    11101 atacgccgca acccgccctc caccagtgcg cgattgttgc ggtcgagcgc caccaggtcg
    11161 gcgaccgtgc cgagcgccac caggtccagc agcgacgcga gatcgacatc gccgcgtttc
    11221 cccatcgctg cccgcaacgc cagcaacagg tagaacacca ccccggcacc gcacaggtat
    11281 ttgctggcga agccatcgcc aggcaggttc ggattgacga ttgcatcggc ctcgggcagt
    11341 cgcggacccg gcaagtggtg gtcggtgacg atgacgcggc agccgcgcgc cttcgcggca
    11401 gcgacgccgg ccagcgatgc aatgccattg tcaacggtga cgatcaggtc gggcgcaggt
    11461 tgcatcgtgt cgaccagcgc tggtgtcagt ccgtaaccgt gcctcgcacg gtcgggaacc
    11521 acgtagccga cataccgcgc acccagcatc cgcaagcccc gcacggcgac agcgcaaccc
    11581 gtcgcgccgt cgcagtcgta atcggctgcg accaggatgg atcggtcgtt agcgatggca
    11641 tcggtcagga gctcgagtgc gcgatcgagg ccagccagcc cggtcggcgg caacagcccg
    11701 gccagccggt ggcccgcgtc gtcggggcag caaaccccgc gcgccgcata gaccctttgc
    11761 acgaccggat gcaccgaagc cagccaatcg cgtggcgccc cacccgctgt ccggcgcacg
    11821 atcctcagcc tggcggcgcc ggtgctcatc cgcgccgcca gaagcggagg cggtaggcgt
    11881 gccgcacccg gaaacgctcg ccggccggta acaccagttc gaccgaatcg aaccgccgcc
    11941 tgccaagggc ttgctcgaag aacgggagcc atctgcttga ttcttcggat gacattacgg
    12001 cgccgagatc gagcagcccg tcgccgaccg tgctctccag cgcttccggc gaaggttccg
    12061 cgatgtcgac tcccgccagc ttggcaagcc cccggaccac gtcatccgtt gttgcgacga
    12121 ccgtcaagtc ggtttccacc gccaccggca gcgagccggc gccccagaac cacagcgcgt
    12181 tgaccggcac cttgccggcg gcgatccgcg ccgcattcac cggatgcgcg tgcaacgcga
    12241 tctgggcccc gctgaacagg tgccgccagc ggcgccctcc ctcgccggcc ggaaggcaag
    12301 ccagcaagtc ggcgcccaac gcgtccgcag ggcgcgtgaa ggcgacgccg gcgagggctt
    12361 ggcccacatg caggcaccac gcggacggtg aatcggccgc aagcgccaat cccgcatcct
    12421 cgaacagcgg cccaagcgta gccgccagcg cctgcgcctc gtcgccggaa aggtccggga
    12481 tcggacatgc catcaaccgg gcgccggtgg gctcgcctcg cacatacgcc ggatccgcgc
    12541 acagccagga tccgcgtgcg gcgtcgccgg catggacatg gtggcgcagc gcggccaccg
    12601 ggaacgcctc gccgggaaag cggaacagtc gtcgcacctg ggcatcccgc gagcctggca
    12661 cgtccggcag gcgatcgccc cttgccagcc agcgccgcag cgccgcgtcg ctgcaggggg
    12721 gcggcgacgg caacaggacg tgcaggacgc tcatgccgtg gccgcttcaa gcgcttcgta
    12781 gcgcgccagc cactcgtttt cgagcgccgt cttttcctcg cgcagggcat tctgctcgcg
    12841 gccgatgtcg gcgaggacgg cggtcggtcc ttcgcagatc gcgggatcgg ccagccgttc
    12901 ctgcagttgt gccagcacgg tgtcgatcgc cgccatgcgc gcctcgatct tctcgagatg
    12961 cttgcgctgg gcacgctggc gctcgcgcat tgctgcggcc tcgcggcgac gctcgcgcgg
    13021 actcgccgcc ggcgaagccg tggtctcgcg cgcgacgggt tcgctgttgc gcgtgcgcag
    13081 ccagcgcgcg taatcatcga ggtcgccatc gaagggcgcg accgcgccgt ccgcgacacg
    13141 ccagaagccg tcgcacacga gtccgagcag gtgccggtcg tgcgccacca gcaccagcgc
    13201 accgggatag tcgttgagcg catcggccaa cgcttcgcgc atgtccaggt cgaggtggtt
    13261 ggtgggctcg tccagcacga gcaggttcgg cttgcgccac gcgatcagcg cgagcgcgag
    13321 gcgcgcgcgc tcgccgcccg agaagccgtc cacgggatcg aacgcgcggt cgcccgcgaa
    13381 atgccattgg cccagccagt cgcgcaggac ctgcgccggc gcgtccggcg ccagttcgcg
    13441 caggtgatcg aagggcgtct cgcccgcacg cagactctcc accgtgtgct gcgcgaagta
    13501 cccgatcacc aggtccttgt gggcctcgcg catccccgcg agcggcggca attcgccgac
    13561 cagtgtcttc accagcgtgg atttgcccgc gccattggga ccaagcaggc cgatgcggtc
    13621 gccggcttcg acgttgaatc ggacgccgga caatatccgt gcaatttcgc ttgtaggagc
    13681 ggcccatggc cgcggccctt cacgctcatc tgcggtagcg tgatcgcggc cctgggccgc
    13741 tcctacatcg ggtgccaggc cgtagccggc gtcgacgtgt tccagcttca gcatcgactg
    13801 cggcacgcgc gccggttcgg ggaaccggaa ttcgaaggta cgttcggcgc gcacggcctc
    13861 ggtgccggcg agcttttcca gacgcttgat gcgcgactgc gcctggcggg ccttggtggc
    13921 cttggcacga aagcggtcga tgaaggactg caagtgcgcg cgctcggcct gctccttcgc
    13981 gaatgcagcc tgctgctggt gcaggcgctc ggcgcgctgc cgcacgaagt cgctgtagtt
    14041 gccggcgtac aagcgcgcac cgccctgctc caggtgcagc acgtgatcga ccgtgccgtc
    14101 gaggaagtca cggtcgtgcg aaatcaccat cagcgtgcgc ggataccggc gcagccactg
    14161 ctcgagccac agaaccgcgt cgaggtcgag atggttggtg ggttcgtcga gcagcagcag
    14221 gtccgagggc gccatcagtg cgcgcgcgag gttcagccgt ccgcgccagc cgcccgagaa
    14281 ttcggccacg gcccgctcgt gttcgccggc cttgaagccc agcccgtgca gcagccggcc
    14341 ggcgcgcgca cggccgtcgt agccacccat cgcgtcgatg cggtgatggg cggccgccgc
    14401 ggcggcgtgg tcgccggctg cctgcgcacg cgcctcgtcg gccagcgccg cggcgagttc
    14461 ggtgtcaccg gccaggacgt agtcgatcgc cgcttcggac agagcgggtg tttcctgtgc
    14521 gaccgaggca atccgcaaac ctgccggtat gtcgagcgcc cccgcatccg cctcgacctc
    14581 gccgaggatg gccgcaaaca ggctcgactt gccggtgccg ttgcgaccgg cgacgccgat
    14641 cttccacccg tcgtgcagga ctgcgtcgac tcctgccagc agcagccgtc caccgcggcg
    14701 aagggcgaag tctcggaaac cgatcacacg tatcgcacgc ccgtgatttc gtagttgcgc
    14761 tcgcccgccg gagcctcgaa ggcgaaactg tcgccttcgc gcttgccgat catcgcgcgc
    14821 gcgatcggcg aggacaccgc gatcaggccc ttcttgatgt cggcttc
//
LOCUS       DASSNH010000241         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1035732, whole genome shotgun sequence.
ACCESSION   DASSNH010000241 DASSNH010000000
VERSION     DASSNH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035732"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(457..1749)
                     /locus_tag="VFK00_08815"
     CDS             complement(457..1749)
                     /locus_tag="VFK00_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593090.1"
                     /translation="MKTLLRKLAVASCAALLLASALAVQAQSLPPEVQVGTRVVNDFE
                     FDWGRDGTYCASCNYGAGNNRLTYIDQQGNVWVSHIDVTTGNFVPGNGKETLVDTNAV
                     PAQAIGNGSEWVGLPSASALVYTRKNESVPGDTCVGLAKVNTSGAWSGGCVPATGGGK
                     LPLGTAIVGDAYPMMSFQDQSDSGGTSIYWQLLSWQSTPQLVLDDNGTLQSRRWIPGT
                     HAMLLAASAPPDQSGNVYQQIFLYSTDDGSLQQLTFDPSSKNSAFMWQAPEFNNTYVF
                     LAVRANGKEIDIYRYLPDGSGASSWRVTKRIQSTPDYPYIISPEPFVYQGRSWVFFEV
                     DAAQRQRGSYAAGQIAISGIQTGISTFRMLTADTGPDIHTRRDPEYYITANGPYIYYN
                     RAVLSDGTPTSEGVFRVDTGLGAPESAAVRAPVANGSR"
     gene            complement(1826..3118)
                     /locus_tag="VFK00_08820"
     CDS             complement(1826..3118)
                     /locus_tag="VFK00_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593091.1"
                     /translation="MKARSRSTVASLYAGLLFALSPGVQAQSLPPEVQVGTKNVIDFE
                     FDWGRDGNYCQTCNYGAGNNRLAYIDSSGNVWVGHVDATTGDFVPTNGKETLVDIDGV
                     PSQAIGNGPEWLASRLASGLVYTRQLEGSGGHKCVGVAHVDTDGVWQGGCMSRTNGAS
                     LPLGTDIVGDYFPMVSFQNSSHPGFNVFWRLLSDGSAAHRVFTGSRAPPSRRWIPGTH
                     DMVLVAGAPPDDSGHVYRQVFLYSTEDDSLQQLTFGASDKNAPFMWQAPEFGSELVLL
                     AVPGNSREADIYRYLLNPDGSSSWKIIKRIHGTPDYPYVASPEPFVYQGKSWVFFEIA
                     AAPRQLGDFAPSQIAISGIEPDVPSFRVLTEDAGPDLHARRDPEYYITANGPYLYYNR
                     YILPAGGGATQTEGVFRVDVGLGPPQAHATYPAMQARH"
     gene            complement(3211..4494)
                     /locus_tag="VFK00_08825"
     CDS             complement(3211..4494)
                     /locus_tag="VFK00_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593092.1"
                     /translation="MRRPGLFVFLAGGLLAVAGSVYAQSLPPEVSVGSRVITDFEFDW
                     GRDGTYCATCNYGDGNNRLTFIDPFGYVWIGHVDVTTGDFVPTNGEGTLVDSDAVPSV
                     AIGNGSEWINLPKASAVVYTRYKYTKSTPDMCLGLAHVGPAGGWTGGCVPGSDNDTLP
                     IGTAIVGDPYPMVSFQNNAGQGFDLYWQLLSRGSTPQLVYPGSAAPPSHRWIPNTHAM
                     VLVAAAPPDGSGNVYRQVFLYSTDGTLQQLTFDPTNKNSPFMWEAPEFNNKYVFLQVP
                     EDSKEVDLYRYLPNRNGPPTWQVYQRIRGTTDYPYIYSPEPFVYQGKSYIFFELASAP
                     HQLGKFAPSQIAMSGIDPNAPSFRVLTGDVAPNLHARRDPEYYITTMGPYLYYNRIIP
                     APPGGQAVNEGVFRVDTGLGPPQAPATLRAARAHP"
     gene            complement(4728..6065)
                     /gene="mnmE"
                     /locus_tag="VFK00_08830"
     CDS             complement(4728..6065)
                     /gene="mnmE"
                     /locus_tag="VFK00_08830"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7593093.1"
                     /translation="MQGAEAIVAVATPPGRGGLGVVRISGGMLDGIAAGVCGGLPEAR
                     RARLGDFLDADGTAIDRGLALYFPAPHSFTGEDVLELHAHGSPVVLDRLLRRVCGLGA
                     RPARAGEFSERAFLNGKLDLAQAEAVSDLIAARSEAQAQAALRSLDGEFSRRVEAVLG
                     SIVRLRVEIEAGIDFSDDASEGASSSLIEQLFAGASELLGTLLVSARRGVRLCDGLHA
                     VIVGEPNVGKSSLMNALAGNERAIVTAIPGTTRDVLRESIVLDGVEVTLADTAGLRES
                     ADAVEGEGVRRARLELARADLALRVIDEPGALREEDLFAGAPMAARRILIVNKVDIRG
                     EPPRREERGGMVTIALSARTGEGVDLLRTELRRLALGSESEGVFSARARHVAALERAR
                     SSLDAARVASESATPELVAEDLRKVQQALGEITGAFSSEDLLGAIFSTFCIGK"
     gene            complement(6083..>6533)
                     /gene="yidC"
                     /locus_tag="VFK00_08835"
     CDS             complement(6083..>6533)
                     /gene="yidC"
                     /locus_tag="VFK00_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7593094.1"
                     /translation="ERFGDDKQKYQQAMMELYKKEKINPVAGCLPALITMPIFFALYY
                     VLIFSVELRHASFLWISDLSAADPYFILPILYALTMLGTSYLTPAQGMDPTQAKIMKV
                     MPVLFSVLFFFFPAGLTLYYVVRGICMLSQQWYVTRKIERADARARA"
BASE COUNT         1146 a         2087 c         2168 g         1132 t
ORIGIN      
        1 atggtcgagt ccggatgttg caggcgagcg agccggacac cagcgacatt gccgctcgag
       61 ccgcgacacg gttgtttcag tcacacgcag ctccgtgcgc gcgacgcccg ggggaacacg
      121 ccgtcgggtt tcccggcgtc gtgaaccgct ggagcggcgg agggtgaggc tcgtggcgat
      181 gggccggacc cctgtatccg ggatgcgaat gttgcaatcc ggcacggcgc gacgaagcct
      241 cgccgtgccg cgcggagata caggcccgcc gtcaccgatg agggacacac ccagcgcgaa
      301 gttgtccgcc gcggcgccaa tgcgacgggc cacccatcaa tgatgggtgg tgcgtgcgat
      361 tgttgccggt caggcgccgc aaatcgaacc gtgcgtctcg cgggagaggg acaacgttcg
      421 gaacaggaga cgcctgcctc gcgacgctcc ttcgattcag cggctgccgt ttgccacggg
      481 cgcgcgcact gccgcagact ccggtgcccc gagcccggtg tcgacccgga acacgccctc
      541 gctcgtcggc gttccgtccg agagtacggc acggttgtag tagatgtagg gaccgttggc
      601 ggtgatgtag tactcgggat cgcgcctggt gtggatgtcc ggcccggtgt ccgccgtgag
      661 catccggaac gtcgatatgc ctgtctggat tccggaaatg gctatctggc ccgccgcgta
      721 gctgccgcgc tgcctttgcg ccgcgtcgac ctcgaaaaac acccaggacc tgccctggta
      781 cacgaagggt tcgggagaaa tgatgtacgg gtaatcgggc gtggattgga tccgcttggt
      841 aacccgccag ctcgacgcgc cgctgccgtc cggaagatac cggtagatat cgatttcctt
      901 gccgttggcc cgcaccgcga gaaacacgta cgtgttgttg aattcgggtg cctgccacat
      961 gaacgccgaa ttcttgctgc ttggatcgaa ggtgagttgt tgcagggagc cgtcgtcggt
     1021 cgaatagagg aagatctgct gatagacatt ccccgactga tcggggggcg ccgacgccgc
     1081 cagcagcatc gcgtgcgtac ccgggatcca gcgacgcgat tgaagcgtcc cgttgtcgtc
     1141 cagcaccagc tgcggcgtgg actgccagga cagcaactgc caatagatgc tggtgccgcc
     1201 gctgtccgac tggtcctgga aactcatcat gggatacgcg tcgcccacga tggcggtccc
     1261 gagcggaagc ttgcccccgc ccgtggcggg cacgcagcca cccgaccacg cgccgctggt
     1321 gttgaccttg gccagcccga cgcaggtgtc gcccggcacg ctttcatttt tgcgggtata
     1381 gaccagcgcg gaggcgctcg gcagtcccac ccattccgac ccgttgccga tggcctgcgc
     1441 cggaaccgcg ttggtgtcga cgagggtttc cttgccgttg cccggcacga agtttcccgt
     1501 cgtcacgtcg atatgactca cccatacgtt gccttgctgg tcgatgtagg tgaggcggtt
     1561 gttgccggca ccgtagttgc agctcgcgca ataggtgccg tcacggcccc agtcgaattc
     1621 gaagtcgttg acgacgcgcg tgcccacctg gacttccggc ggcaaggatt gcgcctggac
     1681 agccagcgcc gatgcaagca acagggccgc gcatgatgcg acggccagct ttcgcaagag
     1741 tgtcttcatg ggtttcccga ccgaactgaa ccgttttgac ttgccgctgc ctgcgcctga
     1801 tgcccccggc agccgcgccc ttttctcagt gcctcgcctg catggcagga tacgttgcgt
     1861 gagcctgtgg aggacccagc ccgacatcca cccggaacac accctcggtt tgcgttgcgc
     1921 ctccaccggc cggaaggatg tagcggttgt aataaagata cgggccgttc gcggtgatgt
     1981 aatactcggg gtcgcgccgg gcgtgtagat ccggccccgc gtcctccgtg agcacgcgga
     2041 atgacggcac atcgggctcg atccctgaaa tggctatctg acttggcgcg aagtcgccga
     2101 gttgccgtgg cgcagcggcg atctcgaaaa atacccacga cttgccctga tagacgaagg
     2161 gttcgggaga cgcgacgtaa gggtaatcgg gcgtcccgtg gatacgcttg atgattttcc
     2221 aactcgacga cccgtccgga ttgagaaggt accggtagat gtcggcctcc ctggaattgc
     2281 ccggcaccgc aagcaggaca agctcgctgc cgaactcggg ggcttgccac atgaaggggg
     2341 cgttcttgtc ggatgcaccg aaggtaagct gctggaggct gtcgtcctcc gtcgaataga
     2401 ggaaaacctg tcggtagacg tggcccgaat catcgggcgg agccccagca accagaacca
     2461 tgtcgtgggt acccgggatc cagcggcgcg atggcggagc ccggctgccg gtaaacaccc
     2521 ggtgcgccgc cgagccgtcc gacaacaacc gccagaagac attgaacccc gggtgggaac
     2581 tgttctggaa gctcaccatg gggaagtagt cgcccacgat gtcggtgccg aggggaagcg
     2641 acgccccgtt ggtacgggac atgcagccac cctgccacac gccatcggtg tcgacatggg
     2701 caacgccaac gcacttgtgg ccgccgctgc cttcaagttg gcgggtatag acgagtcccg
     2761 aggccagccg cgaggccagc cattcggggc cattgccgat tgcctgcgag gggacgccgt
     2821 cgatgtccac caacgtctcc ttgccgttgg tcggtacgaa gtcgcccgtc gtggcatcga
     2881 catggccgac ccacacgttg ccggacgaat cgatgtacgc cagacggttg ttgccggcgc
     2941 cgtagttgca ggtctggcag tagttgccgt cgcggcccca atcgaactcg aagtcgatga
     3001 cgttcttggt ccccacctgg acttcgggcg gcaacgattg cgcctgcacg cccggggaca
     3061 aggcaaacag cagccccgcg tacagcgagg cgaccgtact ccgcgacctt gccttcatga
     3121 gtcctccctg gaatctgatc ggtgctgcct ttgccccggc tccttccgga tagccggcaa
     3181 ccggggcgga caagcctgcc atgccggtat tcaagggtgg gcgcgtgccg cgcggagggt
     3241 tgcgggcgcc tgcggcggac cgagccctgt atccacccgg aatactccct cgttcaccgc
     3301 ctggccgccc ggcggcgcgg ggatgatgcg gttgtagtag aggtagggcc ccatcgtggt
     3361 gatgtagtac tcggggtcgc gccgcgcgtg aagattcggc gccacgtccc cggtgagcac
     3421 gcggaacgag ggtgcgttcg gatcgatgcc ggacatggcg atctggctcg gcgcgaattt
     3481 gccaagctga tggggcgcgg acgcgagctc gaagaatatg taggacttgc cctggtacac
     3541 gaagggctcg ggcgagtaaa tgtacggata gtccgtggtg ccccggatgc gctgatagac
     3601 ctgccaggtc ggcggtccgt tcctgttcgg gaggtaccgg tagagatcga cttccttgga
     3661 gtcttccggc acctgcagga agacatactt gttgttgaat tcgggcgcct cccacatgaa
     3721 tggcgaattc ttgttggtgg ggtcgaaggt gagctgttgc agggtgccat ccgtggaata
     3781 gaggaagacc tggcgatata cgttgccgga tccatcggga ggcgccgccg ccaccagcac
     3841 cattgcgtgc gtattgggaa tccaacgatg cgagggcgga gctgcgctgc ccggatacac
     3901 cagctggggc gtcgatcccc tcgacagcag ctgccaataa aggtcgaaac cttgcccggc
     3961 gttgttctgg aaactcacca tgggataggg atcgcccacg atggcggtgc cgatgggaag
     4021 cgtgtcgttg tcgctccccg gcacgcagcc gcccgtccat cctccggcgg ggccgacatg
     4081 ggcgagcccg aggcacatat cgggcgtcga cttcgtgtat ttgtagcgcg tgtacaccac
     4141 cgccgaggcc ttgggcaagt tgatccattc ggagccgttg ccgatggcga cggagggcac
     4201 cgcgtcggag tcgacgaggg tgccttcacc attggtcggc acgaagtcgc ccgtggtcac
     4261 gtccacgtgg ccgatccaca cgtagccaaa cggatcgatg aacgtcaggc ggttgttgcc
     4321 atcgccgtag ttgcaggtcg cgcagtacgt tccgtcgcgg ccccaatcga actcgaagtc
     4381 ggtgatgacg cgggacccca ccgagacttc aggtggcagg gattgcgcat aaacacttcc
     4441 cgccacggcg aggagcccgc cggcgaggaa gacgaacagc ccaggacgac gcatgaccag
     4501 tccccccaag aacgtgagcc acgtcacgtt ttcggacgct actcccgggg atgttaagct
     4561 ttcgtaaaga cggccaatgg ggggccgtag ccgctcgaac cggcggaatt cacgcctctg
     4621 aacggggtcc cagcgcacag ccatgcgttg tcggggtggc gtcgtttgat actggacccc
     4681 gtgtcagcgg tcatccatgg cctgcacggg atcagcgcat cctgcactca ttttccgatg
     4741 caaaaggtgg agaagatcgc gccgagcaaa tcctcgctcg agaaggcgcc ggtgatttct
     4801 ccgagcgcct gctggacctt gcgcaggtcc tcggccacca gctcgggggt ggcgctttcc
     4861 gatgccacgc gcgcggcgtc caacgatgag cgggcgcgct cgagtgccgc cacgtgccgt
     4921 gcacgtgcgc tgaagacgcc ctcgctttcg ctgccgagtg cgaggcgccg caattccgtg
     4981 cgcaggagat cgacgccctc gcccgtacgc gccgacaacg cgatcgtcac catgcccccg
     5041 cgttcctcgc gccgtggcgg ttcgccgcgg atgtcgacct tgttgacgat gagaatgcgg
     5101 cgcgccgcca tcggagctcc cgcgaacagg tcctcctcgc gcagcgcacc cggctcgtcg
     5161 atgacccgca gcgcgagatc ggcgcgagcg agttcgaggc gcgcgcgccg gacaccttcg
     5221 ccttccacgg cgtcggcaga ctcgcgaagg cccgcggtgt cggcgagggt cacttcgacg
     5281 ccatccagta cgatcgattc acgcagcacg tcccgggtcg ttccgggtat ggcggtcacg
     5341 atcgcgcgtt cgttgccggc cagcgcattc atgagactgg atttgccgac gttgggttcg
     5401 ccgacgatca ccgcgtgcag gccatcgcaa aggcgtacgc cacgacgcgc ggataccagc
     5461 agcgtcccga gtagttccga cgccccggcg aagagctgct cgatcaggct ggacgatgcg
     5521 ccttccgacg cgtcgtcgga gaaatcgatg cccgcctcga tttcgacgcg gagacgcacg
     5581 atgcttccga gcacggcttc gacgcgccgg gagaattcgc catcgagcga acgcagcgcc
     5641 gcctgcgcct gtgcctcgga gcgcgccgcg atcagatcgg agaccgcttc ggcctgggca
     5701 agatcgagct tgccgttgag gaaggcgcgt tcggaaaatt cacccgcccg cgccgggcgt
     5761 gcacccaagc cgcacacacg ccgcagcagg cgatcgagca ccaccgggct gccgtgcgcg
     5821 tggagttcca gcacgtcttc gccggtgaag gaatgcggcg cggggaaata cagcgcgagc
     5881 ccgcggtcga tcgcggtgcc gtcggcatcg aggaagtccc ccagccgcgc ccgccgggcc
     5941 tccggcaggc cgccgcacac gccggccgcg atgccgtcca gcatgccgcc cgatattcgc
     6001 accacgccga ggccgccgcg ccccggcggc gtcgccaccg cgacgatggc ttcggcgccc
     6061 tgcacgtccg cggcgcgccg ggtcaggcgc gcgcgcgcgc gtcggcgcgt tcgatcttgc
     6121 gggtgacgta ccactgctgc gacaacatgc agatgccgcg gacgacgtaa taaagcgtca
     6181 gaccggccgg gaagaagaag aacagcaccg agaacagcac cggcatgacc ttcatgatct
     6241 tggcctgggt cgggtccatg ccctgcgccg gggtcaggta gctggtgccg agcatcgtca
     6301 gtgcgtacag gatcggcagg atgaagtacg gatcggccgc ggacaggtcc gagatccaca
     6361 ggaacgacgc gtggcgcagt tccacgctga agatcagcac gtagtacagc gcgaagaaga
     6421 tcggcatcgt gatcagcgcc ggcaggcagc ccgccaccgg gttgatcttc tccttcttgt
     6481 acagctccat catcgcctgc tggtacttct gcttgtcgtc gccgaagcgc tcc
//
LOCUS       DASSNH010000242        21714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_296131, whole genome shotgun sequence.
ACCESSION   DASSNH010000242 DASSNH010000000
VERSION     DASSNH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 21714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21714
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296131"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VFK00_08840"
     CDS             <1..1278
                     /locus_tag="VFK00_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET7593095.1"
                     /translation="GRFDLAIALGILAAGGQLPHAALQGCEFLGELALSGELRPVPGV
                     LPALLRARHGRRCVIVPVGNAPEAALLGDVDVRVARNLAEVCAHLRGADALPPPPDSQ
                     AEDAFGYPDLADVRGQRQARRALEIAAAGGHHLLMTGPPGTGKSMLAARLPGVLPPLT
                     EAEAIEACAVRSVAGVPFDAGNWRQRPYRAPHHTASSVALVGGGSHPRPGEISLAHNG
                     VLFLDELPEFDRRVLEVLREPMESGRIAISRAAHQAEFPARFQLVAAMNPCPCGYAGD
                     PDGRCRCTPDAIARYRGRISGPLLDRIDLIVDVPRIALAELDSSRGSSAEDSATVRAR
                     VAVARRVATQRAGKPNAALATRELERDCALGSAERALLERAMEKLSLSARAYHRVLRV
                     ARTIADLARCDRIACEHLAEAVQYRRTDAAIQP"
     gene            1359..1928
                     /locus_tag="VFK00_08845"
     CDS             1359..1928
                     /locus_tag="VFK00_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593096.1"
                     /translation="MKFRLLLPMMLALLCCACVTHIRPTVAGNPPPAEPLSHFQHFML
                     QPVKASDAAAHEADAVAKISTYMQQRVGGRLATWENRNQSGRVLDIEPYIVQLKFVSG
                     GARFFAGALAGSSAVLMRVKFVDAQTGRVIADPEFYQRAAAYGGAWSIGGTDNGMLAR
                     IATVVQQYLDRNYGQAVGGPTGLDGTEPD"
     gene            complement(1955..2509)
                     /locus_tag="VFK00_08850"
     CDS             complement(1955..2509)
                     /locus_tag="VFK00_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7593097.1"
                     /translation="MPQFPGSAWRHVFRAWVKASGWRLEGELPDVAKLVLIVAPHSSW
                     WDGIHGLLFKVALGADITFMAKRELFRWPLGGLLRRLGGVPIERRAAHDVVEQMVDRF
                     RANERLWLGIEPEGTRKAVVKWKSGFWHIAREAGVPILPGYFDYPRKVIGLGPLFQPT
                     GDKDADIAALREFYRPFRGRHRGV"
     gene            complement(2566..2985)
                     /gene="arfB"
                     /locus_tag="VFK00_08855"
     CDS             complement(2566..2985)
                     /gene="arfB"
                     /locus_tag="VFK00_08855"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET7593098.1"
                     /translation="MRISSSIDIPDSELTERFLRADGPGGQHVNRTESAVELRFDVAH
                     SPSLPDAVRARVLARRDRRLTDEGVLVIQARRFRDQSRNRDDARARLVEIIRGALTPP
                     KKRVATRPTRASRERRLAGKKQRGAVKRNRGRSWSED"
     gene            complement(3136..3894)
                     /locus_tag="VFK00_08860"
     CDS             complement(3136..3894)
                     /locus_tag="VFK00_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593099.1"
                     /translation="MAEPDWTRPRWSRDEARAMLVYFVFGEFAAEPTLDLSAHGSQGL
                     PQGVEILRIPKERLAHWQGHPLRGALGDILRDGNPAAFETARKAPECLLLRGELADPD
                     SLAYLRDTLGVISALLDAGGIAVVDPQILELFSAEDWRVRYAGGNPASSRRHVAVLCS
                     DGAADHAWIHTRGLRKFARPDISIRGVPREQVQHAGEVAARLAELQVQGMRFGDGSTV
                     DVEGIPGGLTVTRAGSLEDPEFNNTHLEMDWPSA"
     gene            3964..5940
                     /locus_tag="VFK00_08865"
     CDS             3964..5940
                     /locus_tag="VFK00_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7593100.1"
                     /translation="MLNPQQRAAVEYTDGPLLVLAGAGSGKTRVITEKIAHLVARGIV
                     PSGKIAAITFTNKAAREMRERVARRVKGDAAEGLNVCTFHALGLKFLQIEHARAGLRR
                     GFSVLDADDSAGLVKELAGAAVKSDKLFQLRSLLSRLKNEALTPEQALDAARAPRELE
                     AAQLYAAYQKRLAAFNAVDFDDLIRLPVAVLQRDEEARAAWRERIRYLLVDECQDTNA
                     AQYEFLRLLAGGRGAFTCVGDDDQSIYAWRGADPDNLDKLSQDYPTLRVVKLEQNYRC
                     AKRVLRAANALIAHNPHAHPKKLWSAHADGERIRVLECRDAGHEAERVASQIMYLREK
                     FQLAWSDCAVLYRGNHQSRPIEKALQLNRIPYRLAGALGFLDRAEVKDVLAYLRLVAN
                     PDDDAAFLRVVNVPRREIGATTLEKLGELAQSKHLSLLRAARNPAALQQLPARPAAAL
                     AGFVDLVESLVAHAKHAKAVELVEEVLKRTHYAEHLSKGSRDPALQERRLGNVRELME
                     WFRAMDSGKVRASDLAAQLALLNHADKDDGGEAVRLMTLHSAKGLEFRSVHIIGCEDG
                     TLPHEGAIDEGRLDEERRLLYVGITRAKELLHLSYSVQGRRYGQVFKQKPSRFLDELP
                     APELHRDGADPEVEAQTRRDVGAAQLAKIAAMLG"
     gene            6113..7030
                     /gene="lpxL"
                     /locus_tag="VFK00_08870"
     CDS             6113..7030
                     /gene="lpxL"
                     /locus_tag="VFK00_08870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HET7593101.1"
                     /translation="MQAPPPFPRKLLAPRHWPAWLGVAAVRMLARLPVRWLLAGGAAF
                     SAAASPLLRERRHVIARNLELCFPELDAGQRESLRREATREMGRMLGEFALGWFASDR
                     KLARLNVTIEGLHHLESARRQGRGVLLVGGHFSHLELCARLISRQIGIAGMYRHMDSA
                     VFETVVLRARLAYADAMFAKDELRATVKYLKGGGTVWYAPDQDMRGKDSVFAPFFGVQ
                     ASTITATHHLARLSGGAVMPFFHHRTPTGSYALRLEAPLEHFPSGDVIVDTTRVNAAI
                     ERMVREAPAQYLWAHKRFKTRPPGMPPVY"
     gene            complement(7101..8408)
                     /locus_tag="VFK00_08875"
     CDS             complement(7101..8408)
                     /locus_tag="VFK00_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7593102.1"
                     /translation="MSGFFPGAWLRSALLSPLTPIWLVILLLPFGRASEFGTLLCILG
                     TILLLARDRQALAGHAGARLALWLWAAYFVAALVSAPDAVDPSRSWSNVAAYLRYAPL
                     AVYVCFAVRRESRLQALCLATGVVVALWALDAWVQAFTGYSIGGHAQAQRISGIFGAG
                     NLKLGPVLASLSPFVLWAARRWLGRMGLVAAMVFLLGPILLAGSRAAWITYGVALLVF
                     FWVEARRPLRFAAWVLAACVVAAAATGIAWQTSPRFDARMQRTLAAFGDHPHAWNDAL
                     SGRLDIWRVASRMIAAHPVNGVGVRDFRYAYSTYAKPGDHFLVDEPCGEDAGACHPHQ
                     VVLEILGDTGAIGLALWLAGLALALRAWRAAGAAARRRAFPATVALAVTLFPLNTHLA
                     FYSAWWGLLFWWLLALWCAALYADVPDSAPAVLPRMRGAGSGD"
     gene            complement(8464..9636)
                     /locus_tag="VFK00_08880"
     CDS             complement(8464..9636)
                     /locus_tag="VFK00_08880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7593103.1"
                     /translation="MSDAPVPQGFVIEPAASAPRRLTVVQLLPALENGGAERSALEVA
                     RALVAAGHRSVVVSAGGRMVPQLEAEGSEHITLDIGSKSLRSLTRIGTLRRTLAGIGP
                     DIVHARSRLPAWLAWFALRGMRPRPGFVTTVHGLNTPGTWSGIMTRGDRVIAVSQSVR
                     DFVLGHYRRVDTARLTVIPRGIDPAAFPYGYRPEAGWRKAFFEEYPQLAGAPLLTLPG
                     RGTRLKGHADAIALIAGLRERGIEARLLLLGIAERGREAYLREMQELAKTRGVADVVV
                     FSPPRSDVRDVFAASDLVLQLSNKPESFGRVVIEALSLCRPVLGYAHGGVGELLAELY
                     PAGRVPVGDVDRLVVRAAELLGRAPPIPPLQRYHLSDMQSAVLALYEELAEKMHKA"
     gene            complement(9710..9925)
                     /locus_tag="VFK00_08885"
     CDS             complement(9710..9925)
                     /locus_tag="VFK00_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HET7593104.1"
                     /translation="MPSQPASSFPVGEPANAKRRYEVSEADLPLACPLPEMVLWNSHP
                     RIYLPIVDDGGETLCPYCGSTFVLKRS"
     gene            complement(10001..10723)
                     /locus_tag="VFK00_08890"
     CDS             complement(10001..10723)
                     /locus_tag="VFK00_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7593105.1"
                     /translation="MLSTSEIAIRLVLAAVLGGVIGFDRERHTWAAGLRTHMMVCLGA
                     ALTMIVSAFAFSGILQQWPRVVLDPSRIAAQVISGIGFLGAGTIMFLHRENVIRGLTT
                     AAGLWTVAAIGLAVGGGMYIAAAIATGIAWVILAALRPLERRFALRKTLPQIQVGFAD
                     RAPLAAVEALLVDRGLPASTVVTRRVPDDGDEVTIEFARGFDRAKLGELADGLRALPG
                     AQSVSLDVALRGSWIQARGGVR"
     gene            10813..11775
                     /locus_tag="VFK00_08895"
     CDS             10813..11775
                     /locus_tag="VFK00_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HET7593106.1"
                     /translation="MSTPPRTAWVISDGAAGNERQALALASALGASARVLSLPLRAPW
                     AWFAPRRLPGGRLALAARDRAGFTPPWPDLAIGCGRSAALLTRMLRDLSRGDCYTVQI
                     LDPRIDPRHWDAVIAPQHDRLEGPNVLSTLGSLNGIDDAWLASGRAAFPDFEAFPRPR
                     VAVLVGGPRHGVDFDAALAGRLVDTVSSLARNGSVLATTSRRTPAAFAASLRDRFAAF
                     PCAFWNGEGTNPYPGLLAWADCIVVTPDSVNMVSEACATGVPVHVPTSMPLPGKLARF
                     HAALRERGLLTEPGSARGDVPPLRETAAVAAELRDRIAARASQP"
     gene            complement(11809..14655)
                     /gene="glnE"
                     /locus_tag="VFK00_08900"
     CDS             complement(11809..14655)
                     /gene="glnE"
                     /locus_tag="VFK00_08900"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET7593107.1"
                     /translation="MPSLPTTLEHGLSERYAELAAHCRASGVVMHDDAGVAERVRCML
                     LASDFAFEVLRADPGLLTASGLERLRDPAPASARAAPLRHAPDTMAALRRFRRAEALR
                     LVFRDVNGLDEVTDTLAGTTSLYEELIAAALRHAEGRARARHGTPRNAEGVPQALTVI
                     ALGKLGGGELNFSSDVDLVLAYPEGGTTDGARPLDNGEFFARVAREFVRLLTEATEDG
                     IAARVDLRLRPFGEAGPVTASFGAMEQYYQREGRDWERYAWIKARPVAGDLAAGRRLL
                     EILRPFVYRRYFDYTALAGLRDMKALIDAEVARRDLADDVKLGAGGIREIEFTVQLQQ
                     LIRGGRDTSLRARGLLPALQACAARGYLSQRQAVNLREAYLFLRLLENRLQMFADRQT
                     HALPDDAVVRERIARGMGHSGWGPLAAALNRRREIVSAIFAEVLQPRESAGPPDGAHA
                     RAHDGTRLWRRAREASLGEAALAAAGFMPPGPCLEALRRVTGLQGLSARSARRIEHLM
                     PDLIEAAAATSAPADALVRLCRLVQAVSRRSAYLALLQEQPAARARVAGLCAESAFLA
                     ERVIGQPLLLDDVLAPRVEHLARGVSNLRDELAQQLAAAQAGGDPEAVLAAIAEWRGS
                     YRMRLGLAFRDGSLDAIATARALAELADAVVGAVLDIAEHELVIRHGRLPGEGSGIAV
                     LGYGSLGGDELGFNSDLDLVFVYDGTRGEATSDGARPLDGARWYARLAQRVVHWLSAG
                     TRGGRLYEVDTRLRPDGGKSLLVASLPAFIDYQRERAWTWEHQALVRARAVAGDAALG
                     AAFARERGEVVRRERAPEDVVTDVRRMRAEWRAQRDRSDPGHLDLKQGAGALLDIQFL
                     LQGLVLMHAHAHPELAVHADTPRLLDDCARTGALPAAAAAALHVAHSELLARALSATL
                     AGIHRVVPRDLVLDARCRKVLEVARRAGFDFGGA"
     gene            14828..16756
                     /locus_tag="VFK00_08905"
     CDS             14828..16756
                     /locus_tag="VFK00_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HET7593108.1"
                     /translation="MAPASPPRARRHSPGARVALLVLLAVAFVLCTAWLDGGKGIVPP
                     TLWTHPAWLPWWDTPLRMFCNALPGLLLAFVLFAWTRRAGWSFLLAFVLQAVLYGVNA
                     LKVQNLGAPLMPADFRMLGQLGKGGGDLLSGYLPHIALLVVVVLACVAALVAWYRFEP
                     PMLDKRRRVPRAAVSVVLLAGLVTLVAGVPFWRGLYNAKLLGLQPWSPTATREHNGLV
                     ESLLLFHLQYGAAHDKPDVDAALQLMARYEPRIKALGKSTAEAAPAQKPDIVVILSES
                     FFDPTILNGYDKDVDFMPNLRRLAQHGTSGWMHSPAFGGGTIRTEFEVLTGLPLRYFR
                     DVMFPYLQLHQKVIPGIVRLLEQRGYQTVAVHGGDPHFWNRASAFKALGFDKFISEGD
                     FPADDAVHDGKYMSDKSFTDEMLRQLKPDGPPQFMLGISIEAHGPYDQSYGIDTRERD
                     AIPVPPKVTGEAKKELQNYIYHIRHADQQLGRFADALAKRNRPTLLVFFGDHLPAIVP
                     AFQQAGFHDGKGFLVQTVPYLLIDTSELHTATPVTKDVAAWELPGMVLRQAGIHDPWF
                     ALTQLVAPQLEGLTRAPDAPVAPETPEQKALDKGMGNVAELRLEGKLEALWPKAATMA
                     AQPQPSSTASAGARSPGS"
     gene            complement(16871..20095)
                     /locus_tag="VFK00_08910"
     CDS             complement(16871..20095)
                     /locus_tag="VFK00_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7593109.1"
                     /translation="MRAIHFAAAALLAALAFPLQAAVDMVPAPKPSFAQPAISPDGSR
                     IAFVSGGAIWEVPATGGAAHLLVADKGEDSHPLFSPSGDKLAFVSDATGGGDVYVLDI
                     AAGTLTRLTHADGPDEPSGWSHDGNWVYFSSSRDNVGGFNAVYRVRALGGTPMPVSLE
                     AYRNEEQGVPSPDNASIALVGGGMGAFQWWRHGSAHIDHGAIWLRANDASHAYSRLTP
                     DDARADWPMWAPDNQSLYYMSDRSGAENLWHVQRSGSESALTRFTDGRLLWPTISGDG
                     KTIAFERDFGIWTVDTATGKAQRVAITLAGAVSGPEDQHKSLHDDFSDLALSPDGKKA
                     AFIAAGNVYAADAKKGGHAVRVTSEPGVAYGITWAPDNHRIVYGAFRDGSRHLYLYDF
                     DSGKETQLTSGDGEDASPAFSPDGKSLAFLRDGRELELLDVSSRRLRTLTRGHLDLHR
                     PLTSDRPLAWSPDGHWIAVLESGPRMFRNAAAVNVASGKTISLSGLANTFSDDVTFSP
                     DGKTFLFTTGQRTEQGQIASVDLVPRTPHFREDQFRDLFRQTTPEPDHENNQTATDES
                     AKSSSAKGKPSHKPVHVAIEADGIDRRLTLLPTGLDAQSIVASPDGKTLVVTAQVAGR
                     TNLYAWSLDPLAGADKPPVARQLTSTSGDKADAQFSPDGKAVYYLDDGKLHSVELAEG
                     SKAKQIDVDAETDVDFARDKMTAFEQAWTWLRDNFHDPAMNGADWNAVRAKYAPLIAG
                     APTRAAVNRLMNLMVGELDASHSGVRPDAHPDSVTGRLGLIFDTAAYERDGTLRVAEV
                     VPLSPAAIAGIEPGDVLQAVDGRVIRANDNLYALLENRIGKKTTLRLAGKSRTRHVEV
                     KPIDSHTLGVLAYDAWVARNRAYVAKISGGRLGYVHLPDMSEASLHRLYRDLDAQNMT
                     REGVVIDVRNNFGGFVNAYALDVLSRRPYLNMTFRGFDQAQPARSILGQRALERPTVL
                     VTNRVTLSDGEDFTEGYRELGLGKVVGEPTAGWIIYTSNEKLIDGATVRLPFITVTTE
                     DGQPMEMHPRPVDVPISRPLGEAYHDKDSDLDAAAGTLLQQLGR"
     gene            20393..21196
                     /locus_tag="VFK00_08915"
     CDS             20393..21196
                     /locus_tag="VFK00_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593110.1"
                     /translation="MHINPSRIAVLSLACAAALGFSMQATARVHAHGGHRGAGQAAHW
                     RATRFTGPNGGTLGHGERTVRDRQEQTLSRGRATRVTGPEGSTIAHGSKIVRDRDDGT
                     VTRSHGTKATGPNGSTLAHGGKTVYGPDGAVTHNGGTQFNGANGGTATGHNSYTKNPD
                     GSSDFSHTTSGTTANGTDFSHSKDVTKSADGTRTASKETTVDGANGGSLDTSKTSGGG
                     TMTRNTEATTKSGATYTGETTYTKGQGVTHTGTCTDANGNTITCPQKHQ"
     gene            complement(21430..>21714)
                     /locus_tag="VFK00_08920"
     CDS             complement(21430..>21714)
                     /locus_tag="VFK00_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET7593111.1"
                     /translation="NAGEEPSVVLNKIAEGKGNYGYNAATGEYGDMVEMGILDPTKVT
                     RSALQNAASIAGLMITTEAMIGEAPKKEEHNHGGAPGGMGGMGGMGGMDF"
BASE COUNT         3505 a         7682 c         7264 g         3263 t
ORIGIN      
        1 ggccgcttcg acctcgcgat cgcgctcggc atcctcgccg cgggcggcca gttgccgcac
       61 gccgcattgc aaggctgcga attcctgggc gaactcgcgc tgtccggcga gctccgtccg
      121 gtaccggggg tgctgcccgc gctgcttcgc gcccgccacg gccggcgctg cgtgatcgtg
      181 ccggtcggca acgcgccgga agcggcattg ctgggtgacg tggatgtacg cgtggcgcgg
      241 aacctcgccg aggtctgcgc gcacctgcgc ggcgccgatg ccctgccccc gccaccggat
      301 agccaggccg aggacgcctt cggttatccc gatctcgccg acgtgcgcgg ccagcgccag
      361 gcgcggcgtg cgctggaaat cgccgccgcg ggtggccatc acctgctgat gaccggtcct
      421 cccggcaccg gcaaatcgat gctggccgcg cgcctgcccg gcgtcctgcc cccgctgacg
      481 gaagcggaag cgatcgaagc ctgtgcggtg cgctccgtcg cgggcgtgcc cttcgatgcc
      541 ggcaactggc gccagcgtcc gtaccgcgcg ccccaccaca cggcgtccag tgtcgcgctg
      601 gtcggcggcg gttcgcaccc gcgcccgggc gaaatctcgc tggcgcacaa cggtgtcctg
      661 ttcctcgacg aattgccgga attcgaccgg cgtgtgctcg aggtgctgcg cgaaccgatg
      721 gaatccggcc gcatcgcgat ctcgcgcgcc gcacaccagg ccgagtttcc cgcgcgcttc
      781 caactcgtcg cggcgatgaa cccctgccct tgcggctacg cgggggatcc ggacggccgc
      841 tgccgttgca cgccggacgc gatcgcgcgt taccgcggcc gcatttcagg accactgctg
      901 gaccgaatcg acctcatcgt cgacgtgccg cgaattgcac tggccgaact cgattcgtca
      961 cgcggatcgt ccgccgagga ttcggcgacg gtacgggccc gcgtcgcggt ggcgcgccgc
     1021 gtggcgaccc agcgcgccgg caagccgaac gccgcgctcg cgacccgcga actcgagcgc
     1081 gactgcgcct tgggtagtgc ggaacgcgcg ctgctcgagc gcgcgatgga gaaactttcc
     1141 ctgtcggcgc gcgcctacca ccgggtgctt cgggtggcgc gcaccattgc ggatcttgcg
     1201 cgctgcgatc gcattgcgtg cgagcatctg gccgaggccg tgcagtaccg gcggacggat
     1261 gcagcaattc agccgtagca gcgcgcatcc cttctgcgac atgggaagtg gtgtgcatct
     1321 tgcttgcaag tgcgagcgta tccaaagggg gaatccacat gaagttccga ttgctgttgc
     1381 cgatgatgtt ggcgctgctg tgttgcgcat gcgttaccca tatccgcccg actgtcgcag
     1441 gaaatccgcc accggcggaa ccgctgtcgc acttccagca tttcatgttg cagccggtca
     1501 aggcatcgga cgccgcagcc catgaagcgg atgccgttgc caagatatcc acctatatgc
     1561 agcaacgcgt gggtggcagg ctcgcgacgt gggaaaaccg caaccagagc ggccgggtgc
     1621 tggacatcga gccgtacatc gtccagttga aattcgtcag tggcggcgcg cggttcttcg
     1681 cgggcgcact ggctggcagt tcggcggtat tgatgcgggt aaaattcgtc gacgcacaga
     1741 ccggccgcgt gatcgcggat ccggagttct accagcgcgc tgcagcctac ggcggtgcct
     1801 ggagcatcgg cgggaccgac aatggcatgc ttgcgcgcat cgccaccgtt gtgcagcagt
     1861 atctcgaccg caactatggc caggccgttg ggggaccgac gggactcgac ggtaccgagc
     1921 cggattgaag ctcggtggtc gcacgcgccg cgggtcacac cccgcggtgc ctgccccgga
     1981 acggacggta aaactcgcgc aacgcggcga tgtcggcatc cttgtcgccg gtgggctgga
     2041 acaatggacc cagcccgatg accttgcgcg gatagtcgaa atagcccggc aggatcggca
     2101 caccggcttc gcgcgcgatg tgccagaaac cggacttcca tttcacgacc gccttgcgcg
     2161 tgccttcggg ctcgatgccc agccacagcc gctcgttcgc gcggaagcga tccaccattt
     2221 gctcgacgac gtcatgtgcc gcgcgccgtt cgatcggaac gccgccgagc cgccgcagca
     2281 agccgccgag cggccagcgg aacagttcgc gcttggccat gaacgtgatg tcggcgccca
     2341 gcgcgacctt gaacagcagg ccgtggatac cgtcccacca cgatgagtgg ggcgcgacga
     2401 tcagcaccag cttcgcgacg tcgggcagtt cgccctccag tcgccagccg ctggccttca
     2461 cccaggcgcg gaacacatgc cgccacgcgc tgccgggaaa ttgcggcatc gccggcggca
     2521 gcgtccgggg cactggatcc gcgcgaaggt tcccgccgga ggaattcaat cctcactcca
     2581 cgagcggccg cgattgcgtt tcactgcgcc gcgctgtttc ttgccggcga gccggcgttc
     2641 ccgcgaggcg cgggtcggcc tggtcgcgac ccgcttcttc gggggcgtga gcgcgccgcg
     2701 gatgatctcg acgaggcgcg cacgcgcgtc atcgcgattg cgtgactggt cgcggaagcg
     2761 gcgcgcctgg atcaccagca cgccctcgtc ggtcagccgc cggtcgcgtc gtgccagtac
     2821 ccgtgcgcgc acggcgtccg gcagggaggg cgaatgggcg acatcgaaac gcaattccac
     2881 cgcgctttcc gtgcggttga cgtgctgccc gccggggccg tccgcacgca ggaagcgctc
     2941 ggtcaattcc gagtcgggaa tatcgatcga ggacgagata cgcatgctgc aaggttagac
     3001 gattcacgac gaggccgcgt cgcacaatcg ccttcggcga ttctcgccat tccgccgcag
     3061 gcgggatcca gcatcttttc cccgcgacat gacgtcaagc tctcgatcca ccccgatatc
     3121 aggtaaaacg tcacgttacg cgctcggcca atccatctcg agatgcgtgt tgttgaattc
     3181 cggatcttcc agcgatccgg cgcgcgtcac cgtcaggccg ccgggaatcc cctcgacatc
     3241 cacggtggag ccgtcgccaa aacgcatgcc ttgcacctgc aattccgcca ggcgggcggc
     3301 gacctcaccg gcgtgctgca cctgctcccg gggcacgcct cgaatgctga tgtcgggacg
     3361 cgcgaacttt cggagacccc gggtatggat ccaggcatgg tcggccgcgc cgtcgctgca
     3421 caggaccgcg acatgcctgc gagacgacgc ggggtttccg ccggcgtaac gcacgcgcca
     3481 atcctcggcg gagaagagtt ccaggatctg cggatcgacc accgcgatcc cgcccgcgtc
     3541 cagcagcgcc gatatcacgc ccagcgtgtc gcgcaggtac gcaagcgaat cgggatcggc
     3601 caactcaccg cgcagcagca ggcactccgg cgccttgcgc gcggtttcga acgcggccgg
     3661 attgccgtcg cggaggatgt cacccagggc gccgcgcaag ggatgccctt gccagtgcgc
     3721 cagccgctcc ttcgggatgc gcaggatctc gacgccctgc ggcaagccct ggctgccgtg
     3781 cgcggaaagg tcgagggtcg gttcggcggc gaattcgccg aacacgaaat acaccagcat
     3841 ggccctggcc tcgtcgcgac tccaacgcgg acgcgtccag tcgggttccg ccatgtgtcg
     3901 aagtcctcga gaaaccgaaa ggcgacacgg taacattgtc ggctggtttg ccggatcgac
     3961 cgcatgctca acccccaaca acgcgccgcc gtcgagtaca ccgacggccc cctgctggtt
     4021 ctggccggcg cgggctcggg caagacccgg gtgatcaccg agaagatcgc gcacctggtc
     4081 gcgcgcggca tcgtgccttc cgggaagatc gccgcgatca ccttcaccaa caaggctgcc
     4141 cgcgagatgc gcgagcgcgt ggccaggcgc gtcaaggggg atgccgccga aggcctgaac
     4201 gtgtgcacct tccacgcgct gggcctcaag ttcctgcaaa tcgaacatgc acgcgcggga
     4261 ttgcggcgcg ggttctctgt gctggacgcc gacgacagcg ccgggctggt gaaggaactc
     4321 gccggcgccg cggtgaaatc cgacaaactg ttccagttgc ggtcgctgct ttcgcgcctg
     4381 aagaacgagg cgctgacgcc cgagcaggcg ctcgatgccg cgcgtgcgcc gcgcgaactc
     4441 gaagccgcac aactgtatgc cgcctaccag aagcgcctgg ccgcgttcaa cgcggtggat
     4501 ttcgacgacc tgatcaggct gccggtcgcg gtgctgcagc gcgacgagga agcgcgcgcc
     4561 gcatggcgcg aacgcatccg ctatttgctg gtggacgaat gccaggacac caacgcggcg
     4621 cagtacgagt tcctgaggtt gctggccggc gggcggggcg cgttcacctg tgtcggcgat
     4681 gacgaccagt cgatctatgc ctggcgcggc gccgatccgg acaacctcga caagttgtcg
     4741 caggactacc cgacgctgcg ggtcgtgaag ctggagcaga actaccgctg cgcgaagcgc
     4801 gtgctgcgcg cggccaacgc gctgattgcg cacaatccgc atgcgcatcc caagaagctg
     4861 tggagcgcac atgccgatgg cgagcgcatc cgggtgctgg agtgccgcga tgccggccac
     4921 gaggccgagc gcgtcgcgtc gcagatcatg tacctgcgcg aaaagttcca gctcgcgtgg
     4981 tcggactgcg cggtgctgta tcgcggcaac caccagtcac ggccgatcga gaaggcgctt
     5041 cagttgaacc gcatcccgta ccgcctggcc ggtgcgctgg gcttcctcga ccgcgccgag
     5101 gtgaaggacg tgctggcgta cctgcggctg gtcgccaatc ccgacgacga cgcggcgttc
     5161 ctgcgcgtgg tcaacgtgcc gcgccgcgag atcggcgcga ccacgctgga aaaactgggc
     5221 gaactcgcgc aatcgaagca cctgtccctg ctgcgtgccg cgcgcaatcc ggcggcattg
     5281 cagcagctgc cggcgcgccc cgccgcggcg ctggcgggtt tcgtcgattt ggtcgaatca
     5341 ctcgtcgcgc acgccaaaca tgccaaagcc gtcgaactgg tcgaagaggt cctcaagcgc
     5401 acccattatg cggagcacct gtcgaagggc tcacgcgatc cggcgctgca ggaacgcagg
     5461 ctcgggaacg tccgcgaact gatggagtgg ttccgcgcga tggattccgg caaggtgcgt
     5521 gcgagcgacc tggccgcgca gctcgcgctg ctgaaccacg ccgacaagga cgacggcggg
     5581 gaggcagtgc ggctgatgac cctgcattct gcgaaagggc tcgaattccg gtccgtccac
     5641 atcattggct gcgaagacgg gaccctgccc cacgaaggcg cgatcgacga aggccgcctc
     5701 gacgaggaac gccggttgtt gtacgtgggg atcacgcgcg ccaaggaatt gctgcacctg
     5761 agctacagcg tgcagggccg tcgctatggc caggtgttca agcaaaagcc gtcgcgattc
     5821 ctggacgaat tgcctgcccc cgaattgcac cgcgacggtg ccgacccgga agtggaagcc
     5881 cagacccgtc gcgatgtcgg cgccgcgcaa ctcgcgaaga tcgcggcgat gctcgggtga
     5941 acttcttgcc ccttccgcag gaagggcgaa gcgaaggaac gcgcagagcg cgagaagagg
     6001 atgtggcttc cctcgctggc cgctgattcg cacctggaca tcccctgcca cttcgtcgcg
     6061 attcccttag cgtgcgaagg gggtgagccc gccgttgcta tcatcgcccg ccatgcaggc
     6121 gccgcccccg ttcccccgca agctgttggc gccacgccac tggccggcgt ggctcggggt
     6181 ggccgccgtt cgcatgctgg cgcggctccc ggtgcgatgg ctgctggctg gaggcgccgc
     6241 tttcagcgct gccgcttcgc ccttgctgcg cgaacgccgg catgtgatcg cgcgcaacct
     6301 cgagctgtgc tttccggaac tcgatgcggg gcagcgcgag tcgctgcgcc gcgaggcgac
     6361 ccgcgaaatg ggccggatgc tgggcgaatt cgcgcttggc tggttcgcga gcgaccgcaa
     6421 gctagcccgg ctcaacgtca cgatcgaagg cctccaccac cttgaaagtg cgcgcaggca
     6481 aggtcgtggc gtgctgctgg tcggcgggca tttctcgcat ctcgaattgt gcgcgcgcct
     6541 gatctcgcgg cagatcggga tcgcgggcat gtaccgccac atggattcgg cggtcttcga
     6601 gacggtggtg ctgcgcgcgc ggctcgccta tgccgacgct atgttcgcca aggacgagct
     6661 gcgcgcgacc gtgaagtacc tgaagggcgg cggcaccgtc tggtacgcgc ccgaccagga
     6721 catgcgcggc aaggattcgg tgttcgcgcc gttcttcggc gtccaggcct cgacgatcac
     6781 cgcgacccac cacctcgcgc gcctatccgg cggcgcggtg atgccattct tccaccaccg
     6841 cacgcccacc ggcagttacg cgttgcggct ggaagcgccg ctcgagcatt tccccagcgg
     6901 cgacgtcatc gtcgacacca cgcgcgtcaa cgccgcgatc gagcgcatgg tccgcgaagc
     6961 gcccgcgcaa tacctgtggg cgcacaagcg gttcaagaca cgaccgccgg gcatgccacc
     7021 ggtgtattga acggcgggaa gcaggcctgc ccgggtgggc gcggtcgctt ccggttttgc
     7081 tcttccccgt gccctgctcc ctagtccccg ctccccgctc cccgcattcg cggcaacacg
     7141 gccggcgcgg aatccggaac gtccgcgtaa agcgcggcgc accacagcgc cagcagccac
     7201 cagaacagca gcccccacca cgccgaatag aacgcgaggt gcgtgttcag cggaaacaag
     7261 gtcaccgcaa gggcaacggt ggccggaaac gcccgccggc gcgcggctgc gcctgccgct
     7321 cgccacgcgc gcaaggccag cgcgaggcct gccagccaca gggcgagtcc gatcgcgccg
     7381 gtatcgccca ggatctccag cacgacctgg tggggatggc aggcccccgc gtcctcgccg
     7441 cagggttcat caacgaggaa gtggtcgccg ggcttggcgt aggtcgaata ggcgtagcgg
     7501 aagtcgcgca cgcccacgcc gttgaccggg tgcgcggcga tcatcctgga cgcgacccgc
     7561 cagatgtcga gccgtccgct gagcgcgtcg ttccaggcgt gcggatggtc gccgaacgcg
     7621 gccagcgtgc gttgcatccg cgcgtcgaac cgcggcgagg tttgccaggc gatgcccgtc
     7681 gccgccgcgg ccaccacgca cgcggcgagc acccacgccg cgaaccgcaa cggcctgcgc
     7741 gcttcgaccc agaagaacac gagcagcgcc acgccgtagg tgatccatgc cgcgcgggaa
     7801 cccgccagca ggatcgggcc gagcaggaac accatcgcgg cgaccagtcc catcctgccg
     7861 agccaccgcc gcgcggccca cagcacgaag ggcgacagcg acgccagcac cggacccagt
     7921 ttcaggttcc ctgccccgaa gatcccggaa atccgttgcg cctgcgcgtg gccgccgatg
     7981 ctgtagccgg tgaacgcctg cacccaggcg tccagcgccc acaacgcgac caccacgccg
     8041 gtggcgaggc agagtgcctg caggcgcgat tcgcgccgca ccgcgaagca gacatagacc
     8101 gcgagcggcg cgtagcgcag gtaggccgcg acgttgctcc aactccgcga cgggtcgacc
     8161 gcatcgggtg ccgagacgag cgccgcgacg aagtaggccg cccatagcca cagcgccagg
     8221 cgcgcgcccg cgtggccggc gagcgcctgg cgatcccgcg cgaggagcag gatcgtgccc
     8281 aggatgcaca gcagcgtgcc gaattcgctg gcgcgcccga acggcagcag caagatcacc
     8341 agccagatcg gcgtcagcgg cgacagcagc gccgaccgca gccatgcacc agggaagaag
     8401 ccgctcatgg ggaaccgggc atgtcgaaac gcgcatcgta ccggtgcacc cgccggcccg
     8461 cggtcaagcc ttgtgcatct tctcggccag ttcctcgtac agcgccagca ctgccgactg
     8521 catgtccgac agatggtagc gttgcagcgg cgggatcggc ggcgcgcgcc ccagcagttc
     8581 cgcggcgcgc acgaccagcc ggtcgacgtc gccgaccggc acgcgcccgg ccgggtacaa
     8641 ctcggccagc agttcgccga cgccgccgtg cgcgtagccg agcaccggcc ggcacagcga
     8701 cagcgcctcg atcaccacgc ggccgaagct ttccggcttg ttggaaagct gcagcaccag
     8761 gtcggaggcc gcgaacacgt cgcgcacgtc gctgcgcggc ggcgagaaca ccacgacgtc
     8821 ggcgacgccg cgcgtcttcg ccagctcctg catttcacgc aaataggctt cgcggccgcg
     8881 ctcggcgatc ccgagcagca gcaaccgcgc ctcgatgccg cgctcgcgca gccccgcgat
     8941 cagtgcgatc gcgtcggcgt ggcccttcag ccgggtgccg cgaccgggca aggtgagcag
     9001 cggcgcgccg gccagttgcg gatattcctc gaagaatgcc ttgcgccaac ccgcttcggg
     9061 ccggtagccg taagggaacg ccgccggatc gatcccgcgg gggatcaccg tgagccgcgc
     9121 ggtatcgacc cgccgatagt gcccgagcac gaagtcgcgc acggattggg aaaccgcgat
     9181 gacccggtcg ccgcgggtca tgatgccgct ccacgtgccc ggggtattca gcccgtgcac
     9241 ggtggttacg aatcccggcc gcggccgcat gccgcgcagc gcgaaccagg ccagccatgc
     9301 gggcaaccgg gagcgcgcgt gcacgatgtc cgggccgatt ccggccaagg tgcggcgcaa
     9361 agtgccaatc cgcgtcaggc tgcgcagcga cttcgaaccg atgtcgagcg ttatgtgctc
     9421 gctgccttcg gcctcgagtt gggggaccat tcggcccccc gcggagacca ccaccgaacg
     9481 atggccggcc gcgaccaggg cacgcgcaac ctcaagcgcc gagcgctccg cgccaccgtt
     9541 ctccagcgcc ggcaggagct gcacgacggt gagtcgacgg ggggctgagg cggcgggctc
     9601 gatcacgaat ccctgcggaa ccggagcatc ggacatgcac caaaagcttg caagaatcac
     9661 gcccggaatc gcgggtcgga actccgcgaa cgcaggcgcg cggggcggtt taggatcgct
     9721 tcaggacgaa cgtcgatccg cagtacgggc acagcgtttc gccgccatcg tcgacgatcg
     9781 gcaggtagat gcgcggatgc gaattccaca gcaccatttc cggcagcggg catgccaacg
     9841 gcaggtccgc ctcgctcacc tcgtagcggc gcttggcgtt ggcgggttca ccgacgggaa
     9901 aggaggacgc aggctgggat ggcatgctgg gaatccctag gatcactgat gtggattgag
     9961 ccgcgtcgcc gcgcggcgtc ggctcgaatg aagcctgatt ctagcgcacg ccgccgcgcg
    10021 cctgaatcca actgccgcgc agcgccacgt ccagcgacac cgattgcgcg cccggcagcg
    10081 cgcgcaaccc gtcggccaat tcgcccaatt tggcacgatc gaagccccgc gcgaactcga
    10141 tggtcacttc gtcgccgtcg tccggcacgc ggcgggtgac gacggtgctg gcaggcaatc
    10201 cgcggtcgac caacaatgcc tcgacggccg ccagcggcgc acgatcggca aaaccgacct
    10261 ggatctgcgg cagcgtcttg cgcagcgcga accgccgctc cagcggcctg agcgcggcga
    10321 ggatcaccca cgcgatcccg gtggcgatcg ccgccgcgat gtacatcccg ccacccaccg
    10381 cgagcccgat cgccgcgacg gtccacaggc cggccgccgt ggtcaggccg cggatcacgt
    10441 tctcgcggtg caggaacatg atcgtgccgg cgccgaggaa cccgatcccg ctgatcacct
    10501 gcgcggcgat ccgcgaggga tccagcacca cccgcggcca ctgctggagg atcccggaaa
    10561 acgcgaacgc cgagacgatc atcgtcaacg ccgcgccgag gcacaccatc atgtgggtgc
    10621 gcagccccgc cgcccacgtg tgccgctcgc ggtcgaagcc gatgacgccg cccagcacgg
    10681 ccgcgagtac caggcggatc gcgatttcag aggtcgagag caccggccag cccgatccgg
    10741 acgacgctgc gagtctatcg gcatgcggcg cctcccgcat aatcgcggtc gccttcccga
    10801 atcgaatgcc cgatgtccac gccaccacgc actgcctggg tcatcagcga cggcgccgcc
    10861 ggcaacgaac gccaggcgct ggcgctcgcg tccgcgctgg gggcgtcggc acgggtgctg
    10921 tcgctgccgc tgcgcgcgcc gtgggcatgg ttcgcgccgc gccgcctgcc gggcggccgg
    10981 ctcgccctcg ccgcccgcga ccgcgcgggc ttcacgccgc cctggcccga cctcgcgatc
    11041 ggttgcgggc gcagcgcggc gttgctgacc cggatgctgc gcgatctgtc gcgcggcgat
    11101 tgctacacgg tgcaaatcct cgatccgcgc atcgatccgc gccactggga cgccgtgatc
    11161 gccccgcagc atgaccggct cgaaggcccc aacgtgctga gcacgcttgg ctccctcaac
    11221 gggatcgacg acgcgtggct cgcatccggc cgtgccgcgt ttcccgattt cgaagccttt
    11281 ccgcggccgc gcgtcgcggt gctggtcggc ggtccgcggc acggggtcga tttcgacgcc
    11341 gccctcgccg gccgtctggt cgacacggtg agcagcctcg cccggaacgg cagcgtgctc
    11401 gccacgacgt cccggcgtac gccggccgcg ttcgcagcgt cgctgcgcga tcggttcgcg
    11461 gcgtttccgt gtgccttctg gaacggcgag ggaaccaatc cgtatcccgg cctgctggca
    11521 tgggccgact gcatcgtggt gacccccgac tcggtcaaca tggtttcgga agcctgcgcg
    11581 accggcgttc cggtgcatgt gccgaccagc atgccgctgc cgggcaagct cgcgcgcttc
    11641 catgccgcgt tgcgcgaacg cgggctcctg accgaacccg gctccgcgcg cggcgacgtg
    11701 ccgcccttgc gggagacggc ggcggtcgct gccgagttgc gggacaggat cgcggcgcgt
    11761 gcatcgcagc cgtgatcgcg ggcatcgccc gcgagtcacc aggcggcgtc acgcgccgcc
    11821 gaaatcgaaa cccgcgcggc gcgcgacctc cagcaccttc ctgcagcgcg catcgagcac
    11881 caggtcccgc ggcaccaccc ggtggatgcc ggccagcgtc gccgacagcg cgcgtgcgag
    11941 caactcgctg tgtgcgacgt gcagcgctgc agcggcggcg gccggcaatg cgccggtccg
    12001 cgcgcagtca tcgagcaggc gcggcgtatc cgcatgcacc gcaagctccg ggtgcgcatg
    12061 ggcatgcatc agcaccaggc cctgcagcag gaactggatg tccagcaggg cgccggcgcc
    12121 ctgcttgagg tcgaggtggc cggggtcgga acggtcacgc tgggcacgcc actccgcgcg
    12181 catccggcgc acgtccgtga ccacgtcttc gggggcgcgc tcgcgtcgca ccacctcgcc
    12241 acgttcgcgc gcgaaggccg cgccgagcgc ggcgtcgccc gccaccgcgc gcgcccgcac
    12301 cagcgcctgg tgttcccagg tccaggcgcg ctcgcgctgg taatcgatga acgccggcag
    12361 gctcgcgacc agcagcgact tgccgccgtc cggccgcagc ctcgtgtcga cttcgtacaa
    12421 acggccgcca cgggttccgg ccgacaacca atgcaccacc cgctgcgcca gccgggcata
    12481 ccagcgggcg ccatccagtg gcctcgcacc atcgctggtc gcttcgccgc gcgtgccgtc
    12541 gtacacgaac accaggtcga gatcggaatt gaaaccgagt tcgtcgccgc cgaggctgcc
    12601 gtaaccgagc accgcgatgc cgcttccctc gccgggcagc cgtccgtgcc ggatgacgag
    12661 ctcgtgctcc gcgatgtcca gcaccgcgcc gaccaccgcg tcggcgagct cggccagcgc
    12721 gcgcgcggtc gcgatcgcat cgagcgaacc atcgcggaag gcgaggccta accgcatccg
    12781 gtagctgccc cgccattcgg cgatcgccgc gagcacggct tcgggatcgc cgcccgcctg
    12841 cgcggcggcg agttgctgcg ccaactcgtc gcgcaggttc gaaacaccgc gcgcgaggtg
    12901 ttccacgcgc ggcgcgagca cgtcgtcgag cagcaaaggc tggccgatga cacgctcggc
    12961 gaggaacgcg ctttccgcgc acaggccggc cacccgcgcg cgggccgcgg gctgctcctg
    13021 cagcagcgcc aggtaggccg agcgccgcga caccgcctgc accagccggc aaagccgcac
    13081 cagcgcatcg gcgggggccg aggtcgcggc cgccgcctcg atgaggtccg gcatcaggtg
    13141 ctcgatgcgc cgcgccgagc gtgcgctcaa tccctgcaga ccggtaacgc ggcgcagcgc
    13201 ttcgagacag ggaccgggcg gcatgaagcc ggcggccgca agcgcggctt cgccgaggct
    13261 ggcctcgcgc gcgcgccgcc acagccgggt gccgtcatgc gcccgcgcat gcgcgccatc
    13321 gggaggcccg gcggattcgc gcggttgcag gacttccgcg aagatcgccg agacgatctc
    13381 gcgccgtcgg ttcagcgcgg ccgcgagcgg gccccatccg gaatggccca tgccgcgtgc
    13441 gatccgttcg cgcacgacag catcgtccgg caaggcgtgc gtctgtcgat cggcgaacat
    13501 ctgcaagcgg ttttccagga gccgcaggaa caggtacgcc tcgcgcaggt ttaccgcctg
    13561 gcgctgcgaa agatagccac gcgcggcgca ggcctgcagc gccgggagca ggccgcgggc
    13621 gcgcagggac gtatcgcggc cgccgcggat caactgctgc aattgcaccg tgaactcgat
    13681 ttcgcggatg cccccggcgc cgagcttcac gtcgtcggcg aggtcgcgcc gcgcgacctc
    13741 tgcgtcgatc agggccttca tgtcgcgcag gcccgcgagc gccgtgtaat cgaagtaacg
    13801 ccggtacacg aacggcctga ggatctccag cagtcgcctg cctgcggcga gatcacccgc
    13861 gaccggccgc gccttgatcc aggcatagcg ctcccagtcg cggccctcgc gctggtagta
    13921 ctgctccatc gcgccgaacg atgcggtgac cggccccgct tcgccgaacg gccgcaaccg
    13981 caggtcgacg cgcgcggcaa tgccgtcctc ggtcgcttcc gtcagcagcc gcacgaactc
    14041 gcgcgccacc cgtgcgaaga actcgccatt gtcgagcggg cgcgcgccgt cggtcgtgcc
    14101 gccttccggg taggcgagca cgaggtcgac gtcggaggaa aaattgagct cgccgccgcc
    14161 cagcttgccg agcgcaatca ccgtgagcgc ctgcggcacg ccctcggcgt tgcgcggggt
    14221 gccgtgacgg gcacgtgcac ggccttcggc gtgccgcagt gccgcggcga tcagttcttc
    14281 gtacaacgac gtggtcccgg cgagggtgtc ggtaacctcg tcgaggccgt tgacgtcgcg
    14341 gaagaccagc cgcaacgcct ccgcgcgacg gaaccgccgc aacgccgcca tggtgtcggg
    14401 cgcgtgccgc aggggcgcgg ctcgtgccga tgcgggcgcc gggtcgcgca ggcgttcgag
    14461 ccccgaggcg gtgagcagcc ccgggtcggc gcgcagcacc tcgaaggcga agtcgctggc
    14521 cagcagcatg caccgcacgc gttcggccac gcctgcgtcg tcgtgcatca ccacgccgga
    14581 cgcgcggcag tgcgcggcca gctcggcgta gcgttcggac aacccgtgtt cgagcgtggt
    14641 cggcagggac ggcatgccgg cattatcagc gggatcattc cgggccgccg caccgctggg
    14701 tgccggaatg cctgctcgcc attcgcgttc cgatcgtccg aggacgttcc cggcaacagc
    14761 ccggtttgcg ccaatccgtt catccggctt cgggtacgct tgccgctcgc cgcatcccaa
    14821 gaaccgcatg gcccctgcat ctccacccag agcccggcgt cattccccgg gcgcccgcgt
    14881 cgcgctgctc gtgctgctcg cggtcgcgtt cgtgctgtgc acggcgtggc tcgacggcgg
    14941 caagggcatc gtgccgccca ccctttggac gcaccccgca tggttgccgt ggtgggacac
    15001 gccgctgcgg atgttctgca atgcgctgcc gggcctgctg ctggccttcg tgctgttcgc
    15061 gtggacgcgc cgggcgggat ggtcgttcct gctggcgttc gtcctgcagg cagtgctgta
    15121 tggggtgaac gccttgaagg tgcagaacct cggggcaccg ctgatgccgg ccgacttccg
    15181 gatgctgggc cagctcggca agggcggcgg cgacttgctg tccgggtacc ttccgcacat
    15241 cgcgctgctg gtcgtggtcg tgctcgcctg cgtcgcggcg ctggtcgcgt ggtaccggtt
    15301 cgaaccgccg atgctggaca agcgtcggcg cgtcccgcgc gcggcggtct cggtcgtgct
    15361 gctcgcgggc ctggtcaccc tcgtcgccgg cgtgccgttc tggcgcgggc tgtacaacgc
    15421 caagctgctc ggattgcagc cgtggtcgcc gaccgcaacc cgcgagcaca acggcctggt
    15481 cgaatcactg ttgctgttcc acctgcaata cggcgccgcc cacgacaaac ccgacgtgga
    15541 tgccgcgctg caattgatgg cgcggtacga accccggatc aaggcactcg ggaaatcgac
    15601 ggccgaggcg gcgccagcgc agaagccgga catcgtggtc atcctgagcg aatcgttctt
    15661 cgacccgacc atcctcaacg gctacgacaa ggacgtcgat ttcatgccca acctgcgccg
    15721 gctggcgcag cacgggacct ccggctggat gcactcgccc gccttcggcg gcgggaccat
    15781 ccgcaccgaa ttcgaggttc tcaccgggct gccgctgcgc tacttcaggg acgtgatgtt
    15841 cccctacctc cagctccacc agaaggtcat cccgggcatc gtgcggctgc tggaacagcg
    15901 cggctaccag acggttgcgg tccacggcgg cgatccgcat ttctggaacc gcgcgtcggc
    15961 gttcaaggcg ctcggcttcg acaaattcat ttccgagggc gacttccccg ccgacgacgc
    16021 cgtgcacgac ggcaagtaca tgtcggacaa gtcgttcacc gacgaaatgc tgcgccagct
    16081 caagccggat ggaccgccgc agttcatgct cggcatcagc atcgaggcgc acggcccgta
    16141 cgaccagagc tacggcatcg acacccgcga acgtgatgcg atcccggtac cgcccaaggt
    16201 caccggcgag gcgaaaaaag aactgcagaa ttacatctac cacattcgcc acgccgacca
    16261 gcaactcggc cggttcgcgg acgccctggc gaaacgcaat cgcccgaccc tgctcgtgtt
    16321 cttcggggac caccttcccg cgatcgtgcc ggccttccag caggcgggct tccatgacgg
    16381 caagggattc ctcgtgcaga cggtgccgta tctcttgatc gatacctccg agctgcacac
    16441 cgccacgccg gtgacgaagg atgtcgccgc ctgggaattg cccggcatgg tgctgcgcca
    16501 ggccggcatc cacgacccgt ggttcgcgct gacgcaactg gtggcgccgc aactggaagg
    16561 tctgacccgc gcgccggatg cgccggtcgc cccggaaacc ccggaacaga aggcgctcga
    16621 caagggcatg ggcaacgtcg cagagctgcg actcgaaggc aagctggagg cgctgtggcc
    16681 caaggccgcc acgatggcgg cccaaccgca accctcgtcc accgcgagcg ccggcgcgcg
    16741 aagcccgggc tcgtagggcg gacgcctccg ccgcaattcc gcctgcggat gacgagtcgc
    16801 gcgggggcgg tcggcgtccg cggcgcggga cgtggctggc catgcccccg cgccggaaag
    16861 cttcgggacg tcagcgcccg agttgctgca acaacgtccc cgccgccgca tccagatcgc
    16921 tgtccttgtc gtggtacgcc tcgcccaacg ggcgcgatat cggcacgtcc accgggcgcg
    16981 gatgcatttc catcggctgg ccgtcttcgg tggtcacggt gatgaacggc aggcgcacgg
    17041 tagcaccgtc gatgagcttc tcgttgctgg tgtagatgat ccagcccgcg gtgggctcgc
    17101 ccacgacctt gcccaggccc agctcgcgat agccttcggt gaaatcctcg ccatcggaaa
    17161 gcgtcacgcg attggtgacg agcaccgtcg ggcgctccag cgcgcgctgg ccgaggatgc
    17221 tgcgcgcggg ttgcgcctgg tcgaatccac ggaaggtcat gttcagatat ggacggcgtg
    17281 acagtacgtc aagcgcgtag gcattcacga agccgccgaa gttgttgcgc acgtcgatca
    17341 cgacgccctc gcgggtcatg ttctgggcat cgaggtcgcg gtacagccgg tgcagcgacg
    17401 cctccgacat gtccggcagg tgcacgtagc cgagccgtcc gccgctgatc ttcgcgacgt
    17461 aggcgcggtt gcgcgcgacc caggcgtcgt aggcaagcac cccgagggtg tgcgaatcga
    17521 tcggcttcac ctcgacatgg cgcgtgcggg acttgcccgc gagccgcagc gtggtcttct
    17581 tgccgatgcg gttctccagc agcgcgtaca ggttgtcgtt ggcccggatc acacggccat
    17641 ccacggcctg cagcacgtcg cccggttcga tgccggcgat cgccgccggc gacagcggca
    17701 ccacctcggc cacccgcaag gtgccatcgc gttcgtaggc cgccgtgtcg aagatcaacc
    17761 ccagccgccc cgtcaccgaa tcgggatgcg catccggccg cacgcccgaa tgcgaggcgt
    17821 ccagttcgcc gaccatcagg ttcatcaggc ggttcacggc cgcgcgggtc ggtgctccgg
    17881 cgatcagcgg cgcgtatttt gcgcgcaccg cattccagtc cgcgccgttc atcgccgggt
    17941 cgtggaagtt gtcgcgcagc caggtccagg cctgctcgaa cgcggtcatc ttgtcgcgcg
    18001 cgaaatcgac gtccgtttcg gcgtccacgt cgatctgttt ggccttgctg ccctcggcga
    18061 gctccaccga atggagcttg ccgtcgtcga ggtagtacac ggccttgccg tcgggactga
    18121 attgcgcatc ggccttgtcg ccgctggtcg acgtcaactg gcgggcgacc ggcggcttgt
    18181 ccgcgccggc aagcggatcg agcgaccatg catacaggtt ggtgcgaccg gcgacctgcg
    18241 cggtgaccac cagcgtcttg ccgtccgggc tggcgacgat cgactgtgca tcgagacccg
    18301 tcggcagcag cgtcaagcga cgatcgatcc cgtcggcttc gatcgccacg tgcaccggct
    18361 tgtgcgacgg cttgcccttc gcggaggacg acttcgcgga ttcgtccgtg gccgtctggt
    18421 tgttttcgtg gtcgggctct ggcgtcgtct ggcggaacag gtcgcggaac tggtcctcgc
    18481 ggaagtgcgg cgtgcgcggc accaggtcga cactggcgat ctgaccctgc tcggtgcgtt
    18541 ggccggtggt gaacaggaag gtcttgccgt ccgggctgaa ggtgacatcg tcggaaaacg
    18601 tgttggccag tccgctcaag gaaatcgtct tgccactcgc cacgttcacg gccgccgcgt
    18661 tgcggaacat ccgggggccc gactcgagca ccgcgatcca gtggccgtcc ggcgaccatg
    18721 ccagcggacg gtcggacgtg agcgggcgat gcaggtcgag gtggccgcgg gtgagcgtgc
    18781 gcaggcggcg gctcgataca tccagaagtt ccagttcgcg gccgtcgcgc aggaacgcca
    18841 gcgacttgcc gtccggcgaa aacgcgggcg aggcgtcttc gccgtcgccg ctcgtcaact
    18901 gcgtctcctt gcccgaatcg aaatcgtaga gatagagatg ccggctgccg tcgcggaacg
    18961 cgccgtacac gatgcgatgg ttgtccggtg cccaggtgat gccgtaggcc acgcccggtt
    19021 cgctcgtgac gcgcaccgcg tggccgccct tcttcgcatc ggccgcgtag acattcccgg
    19081 ccgcgatgaa agcggccttc ttgccatccg gcgacaatgc gaggtcgctg aaatcgtcgt
    19141 gcaggctctt gtgctggtcc tccggtcccg agaccgcgcc ggccagcgta atggcgaccc
    19201 gctgtgcctt gccggtggcg gtgtcgacgg tccagatgcc gaaatcgcgc tcgaacgcga
    19261 tcgtctttcc gtcaccggaa atggtcggcc acagcagccg gccatcggtg aatcgggtca
    19321 gcgcgctttc gctgccgctg cgttgcacgt gccaaaggtt ttcagcgccg gaacgatcgc
    19381 tcatgtagta gagcgactgg ttgtccggcg cccacatcgg ccagtccgcg cgcgcatcgt
    19441 ccggcgtcag ccgcgaatac gcgtgcgaag catcgttcgc gcgcagccag atcgcgccgt
    19501 ggtcgatgtg cgcgctgccg tgtcgccacc actggaacgc acccattccg ccgccgacca
    19561 gcgcgatgct ggcattgtcc ggtgaaggca cgccctgttc ctcgttgcgg tacgcctcca
    19621 gcgacaccgg catcggcgtg ccacccaacg cgcggacccg gtacaccgcg ttgaagccgc
    19681 ccacgttgtc gcgcgaggag ctgaaataga cccagttgcc gtcgtgtgac cagccgctcg
    19741 gttcatcggg gccgtcggcg tgggtgagcc gcgtcaacgt gccggccgcg atgtcgagaa
    19801 cgtagacgtc gccaccgccg gtcgcgtcgg acacgaacgc gagcttgtcg ccggacggcg
    19861 aaaacagcgg gtgcgagtcc tcacccttgt cggcaaccag caaatgcgcc gcgccgcccg
    19921 tcgccggaac ttcccagatc gcgccgccgg agacgaacgc aatgcggctg ccgtccggcg
    19981 aaatcgccgg ctgcgcgaaa ctcggctttg gcgcgggcac catatcgacc gccgcctgca
    20041 acggaaacgc cagcgccgcc agcaacgccg cggcggcaaa atggatcgcg cgcatcggga
    20101 actcccctgg accgtatggt cgcgggagtg tagccatgcc cacgcgaccg gccatgggcc
    20161 ataggtcgtg gcgagcgtga tggcgtcccg cagcgatctt tgtccgatcg ccgccgcgcc
    20221 cgccgttcag cttcggcggg taaaccgatg tttccgtggc gcgttatagc cagtgaagga
    20281 acactgaccc gtcaccacga ctggacaagc tccgttcatt cgcatcgcgc aacactccta
    20341 gcgcaccgca ccagcgtctc gcggtatccg acctccctcc ccggagcaca ccatgcacat
    20401 caatccatcc cgtattgctg tcttgagcct cgcatgcgcc gcagccctcg gcttcagcat
    20461 gcaggccacc gcacgtgtcc acgcccacgg cggtcaccgc ggcgcggggc aagccgccca
    20521 ctggcgcgcc acccgcttca ccggccccaa cggtggcacg ctcggccacg gcgagcgcac
    20581 cgtccgcgat cgccaggaac agaccctgtc gcgcggccgc gccacgcgcg tgaccggccc
    20641 ggaaggaagc acgatcgccc atggcagcaa gatcgtacgc gatcgtgatg acggcacggt
    20701 cacccgttcc catggcacca aggccaccgg tccgaacggc agcaccctcg cgcacggcgg
    20761 caagacggtc tatggccccg acggggcggt gacgcacaat ggcggaaccc agttcaacgg
    20821 tgcgaatggc gggaccgcca ccggccacaa cagctacacg aagaatccgg atggctcatc
    20881 ggatttcagc cataccacca gcggcaccac ggccaacggg accgacttca gccacagcaa
    20941 ggacgtcacc aagtcggccg acggcacacg caccgcaagc aaggaaacga cggtggacgg
    21001 cgcgaacggc ggcagcctgg acacctccaa gacctccggc ggcggcacca tgacccgcaa
    21061 cacggaggcg accacgaagt ccggcgccac ctacaccggt gagaccacct acaccaaggg
    21121 tcaaggcgtg acccacaccg gcacgtgcac ggacgccaat ggcaacacga tcacctgccc
    21181 ccagaaacac cagtgatcgt cgaccgttga tcagcggacg gagagccatg gcggcagcgt
    21241 agtatcacca ggacaaacct ggctcacgct tggccagccg cacaacaaaa agccccgcga
    21301 ttgctcgcgg ggcttttttg cgacgtataa cgcaacgcgg gcacgtaccc tcacccgccg
    21361 ctgcgctcgc gctacgcgcg cgttcgcttc gctccgcctc tcccggaggg agaggggagc
    21421 agcacgaact cagaagtcca tgccgcccat gccacccatg ccgcccatcc caccaggtgc
    21481 gccgccatgg ttgtgctctt ccttcttcgg cgcttcgccg atcatggctt cggtggtgat
    21541 catgaggccg gcaatcgagg ccgcgttctg cagcgccgaa cgggtgacct tggtcggatc
    21601 gaggatgccc atctcgacca tgtcgccgta ttcgccggtg gcggcgttgt agccgtagtt
    21661 gcccttgcct tcggcgatct tgttcagcac caccgacggc tcttcgccgg cgtt
//
LOCUS       DASSNH010000243         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1653131, whole genome shotgun sequence.
ACCESSION   DASSNH010000243 DASSNH010000000
VERSION     DASSNH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653131"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFK00_08925"
     CDS             <1..543
                     /locus_tag="VFK00_08925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7593112.1"
                     /translation="RGSYNRKLLEACGHRAPGNLRIKLFDGLADVPMFNEDMESESPL
                     HAGVGRLRQAVAGADGLLIATPEYNQTTPGMLQNAIDWLSRPLPADLLVGKPVAVLGA
                     SAGRWGTRLAQAALRHTLTATEAHVMPAPMLFVRNAEQVFDDEDALRDAGTSEALREF
                     LLAFSDWIELVGSRRAAVAA"
     gene            complement(573..>2511)
                     /locus_tag="VFK00_08930"
     CDS             complement(573..>2511)
                     /locus_tag="VFK00_08930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET7593113.1"
                     /translation="SHETEVAQPGYYAVTLATSGVRVELTAGKRVGWHRYTFPKGQDA
                     HVLLDLRPSIYDYPGKVLWARLRVRADGTVTGYRETRGWAPGRQLYFAIRFSQPMASH
                     QLVNRETDVPYKGFQAPAANPDGNDAVAGKQLEGAFEFGKLAKPLLVKVAISTVSADN
                     AVANLDKDGKGWDFDARRAEASAAWAKALSAIDVDAPPDVEKNFYTAMYHALIAPNLA
                     MDANGEYRGPDHQVHKADGFTFYSSWSLWDVYRAQQPLTAFLQPPRRVNDFVNSLIAA
                     QQESPFGILPVWAYQGLETWCMIGYHAVPVIADAYMKGFRGFDADAALKAMVASATYA
                     PYGGLGDYMKHGYVPIDKEPEAASKTVEYAFDDWTIARMAQAMGRNDVAAKFFKRAAN
                     WRNVFDPKTGFVRARKSNGEFRTPFDPAAVGYGSDYTEGNAWEYSWYEPQDVAGLIHE
                     LGGDAGLIEKLDGVFDAKVDPKEFAHVEDISGLIGYYAQGNEPSHHIAYLYDYAGQPW
                     KTQERLAQIMHTQYAPTPTGLAGNDDLGQMSAWYIFTALGFYPVAPASNQYVIGRPFV
                     RRAVMHLPNGKTFTITTDGLGQDHPYVGSVKLDGEPLTRDYLTYAEITRGGTLHFTMQ
                     AEPNKDWATRPQDQPYSMTAY"
BASE COUNT          398 a          817 c          832 g          464 t
ORIGIN      
        1 cgtggcagtt acaaccgcaa gctgctggag gcctgcggcc accgcgcgcc cggcaatttg
       61 cggataaagc tgttcgacgg cctcgcggac gtgccgatgt tcaacgaaga catggaatcc
      121 gaaagcccgc tgcacgctgg cgtgggccgg ctgcgccaag ccgtggccgg cgcggacggg
      181 ctgttgatcg caacgccgga gtacaaccag acgacgccgg ggatgttgca gaacgccatc
      241 gactggctgt cccggccgtt gcccgcagac ctcctcgtcg gcaagccggt ggcagtcctg
      301 ggcgcttcgg ccgggcgctg gggcacgcgg ctcgcccagg ccgcattgag gcacacgctc
      361 accgcgaccg aagcgcacgt gatgcccgcg cccatgctgt tcgtgcgcaa cgccgagcag
      421 gttttcgacg acgaagatgc cttgcgcgac gccggtacgt cggaggccct gcgcgagttc
      481 ctgctcgcct tttccgactg gatcgagctg gtcggcagcc ggcgcgcggc ggtcgcagcc
      541 tgaccctcga ccgccggcgc attgctcgcc gatcaatacg ccgtcatcga gtacggctgg
      601 tcctgcggtc gcgttgccca atccttgttc ggctcggcct gcatcgtgaa gtgcagcgtg
      661 ccaccgcggg tgatctccgc atacgtcagg tagtcgcgcg tgagcggctc gccgtccagc
      721 ttgaccgagc cgacataggg atgatcctgg ccgagcccgt ccgtggtgat tgtgaaggtc
      781 ttgccgttgg gaaggtgcat caccgcgcgg cgcacgaacg gacggccgat cacgtactgg
      841 ttgctggcgg gggcaaccgg atagaacccg agcgcggtga agatgtacca cgccgacatc
      901 tggccgaggt cgtcgttgcc cgccagaccg gtcggcgtgg gcgcgtattg ggtgtgcatg
      961 atttgggcga gccgttcctg cgtcttccag ggttgtccgg cgtagtcgta gaggtacgcg
     1021 atgtggtggc tgggttcgtt gccctgcgcg tagtagccga tcaggcccga gatgtcctcg
     1081 acgtgggcga attccttcgg atccactttg gcgtcgaaaa ccccgtccag tttttcgatc
     1141 aagccggcgt cgccgccgag ttcgtggatg aggcccgcga cgtcctgtgg ctcgtaccag
     1201 gaatattccc acgcgttgcc ctcggtgtag tcgctgccgt agccgaccgc cgccggatcg
     1261 aacggcgtcc ggaattcacc gttggatttg cgcgcacgca cgaagccggt tttcggatcg
     1321 aacacgttgc gccagttcgc ggcgcgcttg aagaacttcg ccgcgacatc gttgcggccc
     1381 attgcctggg ccatgcgtgc gatggtccag tcatcgaagg cgtattcgac cgtcttggat
     1441 gccgcctccg gttccttgtc gatgggcacg tagccgtgct tcatgtagtc gccgaggccg
     1501 ccgtagggcg cataggttgc gctcgcgacc atcgccttca gggcggcgtc tgcatcgaaa
     1561 ccgcggaaac ccttcatgta ggcatcggcg atcaccggca ccgcgtggta accgatcatg
     1621 caccaggtct cgaggccctg gtacgcccac accggcagga tgccgaacgg actttcctgc
     1681 tgggcggcaa tcagcgaatt cacgaaatcg ttgacgcgcc ggggcggctg caggaatgcc
     1741 gtcagcggct gctgcgcgcg ataaacatcc caaagcgacc agctcgaata gaaggtgaaa
     1801 ccatcggcct tgtgcacctg gtggtcgggg ccgcggtatt cgccgtttgc gtccatcgcg
     1861 aggttgggcg cgatcagcgc gtggtacatc gccgtgtaga agttcttctc gacgtccggc
     1921 ggcgcgtcca catcgatcgc cgagagtgcc ttcgcccacg ccgcactcgc ctcggcgcgg
     1981 cgcgcatcga aatcccaacc cttgccgtcc ttgtcgagat tcgcgacggc gttgtcggcg
     2041 ctcacggttg aaatcgccac cttcaccagc aagggcttgg caagcttgcc gaactcgaac
     2101 gcgccctcga gctgcttgcc cgcgaccgcg tcgttgccgt ccggattcgc ggccggggcc
     2161 tggaagccct tgtacggaac atcggtttcg cggttgacca gctggtgcga ggccatcggt
     2221 tgcgaaaacc ggatcgcaaa atacagttgc cgccccggcg cccaaccgcg cgtttcgcga
     2281 tagccggtga cggtgccgtc ggcacggact cgcagccgcg cccacagcac cttgcccggg
     2341 tagtcataga tgctcgggcg aagatccagc agcacgtgcg cgtcctggcc cttcgggaac
     2401 gtatagcgat gccagccgac gcgcttgccc gcggtcagtt ccacgcgcac gccggaggtc
     2461 gccagcgtca ctgcgtagta gcccggctgg gcgacttcgg tctcgtggct g
//
LOCUS       DASSNH010000244        11081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1851564, whole genome shotgun sequence.
ACCESSION   DASSNH010000244 DASSNH010000000
VERSION     DASSNH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851564"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..897)
                     /locus_tag="VFK00_08935"
     CDS             complement(52..897)
                     /locus_tag="VFK00_08935"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7593114.1"
                     /translation="MNDVLIDAETLAGLPSESVLIVDCRFALADPGQGEREFLEAHIP
                     GAVYASLDRDLSDLSKQGLGRHPLPEPEVFAQTLSRWGWRLGMRVVAYDDAGGALAAA
                     RLWWMLDAAGIAASVLDGGWRAWREAGLPTESGEAVPLPSAPVDFGFDPRRVVFYEEL
                     ENLRQGHATLLVDARAAPRFRGESEPIDRVGGHVPGAANRPFSQNLDSNARFKPAAAL
                     REEWGEALGGRVPRDVVHMCGSGVTACHNLLAMESAGLHGSRLFAPSWSGWVSDPSRP
                     VARGS"
     gene            complement(1017..1819)
                     /locus_tag="VFK00_08940"
                     /pseudo
     CDS             complement(1017..1819)
                     /locus_tag="VFK00_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
     gene            complement(1970..2707)
                     /locus_tag="VFK00_08945"
     CDS             complement(1970..2707)
                     /locus_tag="VFK00_08945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7593115.1"
                     /translation="MTHNVLETGMGRVVVAMVLIAGTLWALDAAAAQTAKPETSPAKR
                     AELSYALGYEAGLDLGRRKHDVDIATVLRGVQDGYSRKKPAYPPQEMQQVIRAMQQQM
                     IADARAAYQKLAAANLRKSHEFFARNQQKPGVVTLASGVQYKVLDGGSGDKSPTVDSK
                     VTLQYRGAFLDGTVFDNTEDRGKPVVFPVNQMIPGWRQIITRMHIGDHWQVFIPPEQA
                     YGIRGEPPRIGPNEALVFDIRLLGVEP"
     gene            complement(2787..3620)
                     /locus_tag="VFK00_08950"
     CDS             complement(2787..3620)
                     /locus_tag="VFK00_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET7593116.1"
                     /translation="MDSESYQREIEAWHAARVERLTAPRGWLSLVGLEWLQPGVNRIG
                     SAADNDVVLAEAPAHLGSIEWHADGSLSLTIDSAADATVDGRRTAHAALLDDSHPAPT
                     EVAFGTVNFVAIERGGRKGLRVRDSEAATRTRFSGIDRFPVDPAWRIVAEWQALDPPF
                     ALATGTVIGTIERYPAPGKAVFERDGRTFELFPVLEAPGDRQLFLIFGDATSGKETHG
                     AARFLYADMPSDGRIVLDFNKAYNPPCAFTAYATCPLAPPENRLDLAVRAGEKKYREK
                     Q"
     gene            complement(3703..5496)
                     /gene="mutL"
                     /locus_tag="VFK00_08955"
     CDS             complement(3703..5496)
                     /gene="mutL"
                     /locus_tag="VFK00_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7593117.1"
                     /translation="MTSIRPLAPELINQIAAGEVIERPASVVKELAENSLDAGAARID
                     IDIEQGGMRLIRVRDDGGGIAAAELPLAMAPHATSKIASFDDLTHVASLGFRGEALAS
                     IASVSRTSITSRRHSDAHAMRLDGGAAQPQPSAHPPGTTVEVRDLFHAVPARRKFLRS
                     ERTEFGHIEELVRALALVRPDVEFRLTHNGKPVRILKGAADDAAQLTRVGELLGDAFA
                     ANCLRIDHSAAGLRLAGWIGLPTASRAQADQQYFHVNGRLVRDRSVAHAVRQAYADVL
                     YHGRHPAFVLFLELDPAAVDVNVHPAKREVRFRDTRLVHDFLFHALHEAVAQTRAGDA
                     ELGPGTFSVAEDRLLPGSRVENVPGPIFPARQYGLGIRDAPTDPYAALLGADHGSGPA
                     SVGLQQAEAGEAPPLGYALAQLAGIYILAENAHGLVLVDMHAAHERVTYEKLKTARDA
                     NRVRSQQLLVPEAIAVSEREATAAEEHAAMLAACGFELDRSGPRQVTARRIPALLEGA
                     DASGLARDVLAELAEHGSSRRLEELQNELLSTMACHGSVRAHRRLTLPEMNALLREME
                     STERSGQCNHGRPTWTQLSVAELDKLFLRGR"
     gene            complement(5738..7147)
                     /gene="ppnN"
                     /locus_tag="VFK00_08960"
     CDS             complement(5738..7147)
                     /gene="ppnN"
                     /locus_tag="VFK00_08960"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HET7593118.1"
                     /translation="MQDVATPRRARPATVDAQIVPAGTLDVLSRNEIARLRDATASGL
                     HGLLRSCTLAVLTSGVVQDDARATLDRYPDFDIQVLQQDRGVKLDLRNAPAQAFVDGK
                     IIRGTNELLFAVVRDIVYVATQIEAGQFDLADSSGITHAVFDILRNARVLRARFDPNL
                     VVCWGGHSISREEYDYSKAVGYQLGLRGMDICTGCGAGAMKGPMKGATIAHAKQRHYE
                     NRYIGLTEPGIIAAESPNPIVNSLVILPDIEKRLEAFVRTGHGFVVFPGGVGTAEEIL
                     YLLGILLDPRNADLPFPLVFTGPAVAASYFEQIDTFLKLVLGEAAARRYRIIIDDPVA
                     VAHAMQHGIEDVRNHRVEQHDAFFFNWEMEIDLSFQQPFVATHEAMAALDLDRGRAPH
                     ALAADMRRAFSGIVAGNVKEEGMQRIERHGPYEIHGDPAFMRALDELLQGFVAQHRMK
                     LPGSKYVPCYRIVGGDAID"
     gene            complement(7271..7900)
                     /locus_tag="VFK00_08965"
     CDS             complement(7271..7900)
                     /locus_tag="VFK00_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET7593119.1"
                     /translation="MQTQMTMVSACITLFLVLDAPGNVPFFLSILKDVPPRRRRWVMV
                     RELLIALAVLVAFLLGGRELLSIMQLRQEAISIGGGVVLFLIAIKMVFPPREGGIFGP
                     VGQGEPFIVPLAIPGVAGPSAMATLLLLTSAQPGRQVEWGVALLIAWLATAVILLGST
                     WLFKFLGESVLAAVERVMGMLLIALSAQMILGGVEGYLVRSSVIAASLG"
     gene            complement(7903..8688)
                     /locus_tag="VFK00_08970"
     CDS             complement(7903..8688)
                     /locus_tag="VFK00_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7593120.1"
                     /translation="MSLPVARPPVDWEPAADALAERIVLITGAAGGLGRAAALACAQA
                     GATVVLADRKTRALEPVYDEIAALPGAPQPVLQPVDLQGASPLEYQTFAESIERQLGR
                     LDGLVHAAVKCEGLTPLAMLSPDEWMTTLHVDLSAPFLLTQACMPLLLAREDSAVVFV
                     LDDPERMGRAHWGAYGVAKAGLERLASILHDETDSTSLRVHALLPAPMRTALRRTVWA
                     GEDPAEVATPDAAARAVLYLLSPAGLAVRGKLLDLRVPEPHGG"
     gene            complement(8685..9014)
                     /gene="grxD"
                     /locus_tag="VFK00_08975"
     CDS             complement(8685..9014)
                     /gene="grxD"
                     /locus_tag="VFK00_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET7593121.1"
                     /translation="MDVIERIKHTLDEHSIVLYMKGTPDFPMCGFSMRTSQALNATGA
                     EYFSVNVLEDPEIRANLPRYSNWPTFPQLYINGELIGGCDITLELYESGELQRMVGEA
                     TKQPTER"
     gene            9124..9702
                     /locus_tag="VFK00_08980"
     CDS             9124..9702
                     /locus_tag="VFK00_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET7593122.1"
                     /translation="MAIELPPLPYERNALAPHISEETLEYHHDKHHKAYVDNLNKQIE
                     GTDFADMNLEDIVKKATGGMFNNAGQAWNHNFYWNCLSPNGGGEPSGKLADAIKKAFG
                     DIGKFKEEFAKIATGTFGSGWAWLVQRTDGSLGLVSTPNAGTPITGSDRPLLTCDVWE
                     HAYYIDYRNARAKYVDAYWSVVNWDFATTQMA"
     gene            complement(9768..>11081)
                     /gene="glmM"
                     /locus_tag="VFK00_08985"
     CDS             complement(9768..>11081)
                     /gene="glmM"
                     /locus_tag="VFK00_08985"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7593123.1"
                     /translation="GVRGRVGEWPITAEFMLKLGRAAGVVLGNGKRPLVLIGKDTRVS
                     GYMFEAALESGLVAAGANVGLLGPLPTPAVAQLTRALGASAGIVISASHNPYQDNGIK
                     FFSATGEKLDDDVEREIEREIDAPFTTVAPEGLGKARRIDDAVERYARFCLGTLQAPP
                     DLSGLHIVLDCAHGATYAVAPKVFESLGARITRIGCDPDGFNINREVGSTHPVALRGA
                     VIEQGADLGIAFDGDGDRVQMIDRNGELADGDDLLYVLARDWLEDGHLRGPVVGTLMS
                     NYGLERALTGAGVKFLRANVGDRYVLQQLKQHHGVLGGETSGHILCLDRASTGDGIVS
                     ALAVLDAVKRSGLDLSEARADLKKLPQITRNVRVSGGRQLVAQPAVQHALEEVQVVLH
                     GRGRVVLRPSGTEPLVRVTIEGEDADEVQALASKLATAVESAAAA"
BASE COUNT         1854 a         3827 c         3603 g         1797 t
ORIGIN      
        1 ggatcatgag gaagggtgct gcccgccgat agccccgagc agcggacccg cctacgaacc
       61 tcgcgccacc ggacgcgagg gatcgctcac ccagccgctc catgacggcg cgaacagccg
      121 cgagccatgc aggcccgccg actccattgc cagcaggttg tggcaggcag tcacgcccga
      181 accgcacatg tgcacgacgt cccgcggtac gcgcccgccg agcgcctcgc cccattcttc
      241 gcgcaacgcg gcggccggct tgaagcgtgc gttggaatcg aggttctgcg agaacgggcg
      301 gttggccgcg ccgggcacgt gcccgccgac ccggtcgatc ggttcggatt cgccgcggaa
      361 ccgcggcgcc gcacgcgcgt cgaccaacag tgttgcgtgt ccctgccgca agttctcgag
      421 ttcctcatag aacacgaccc gtcgcgggtc gaaaccaaaa tcgaccggtg cggacggaag
      481 tggtacggcc tcgcccgact cggtcgggag ccccgcttcg cgccaggccc gccagccacc
      541 gtccagcacg ctcgcggcaa tgccggcggc gtcgagcatc caccatagcc gcgcggcggc
      601 gagtgcgccg ccggcatcat cgtaggccac cacccgcatt cccaatcgcc agccccagcg
      661 cgagagggtc tgcgcgaaga cctccggctc gggcagcgga tgccttccca gcccctgctt
      721 cgacaggtcc gacaggtcac ggtcgaggct cgcatacacc gcgccgggaa tgtgcgcctc
      781 caggaactcg cgctcgccct gccccggatc ggccaatgcg aaccggcaat ccacgatgag
      841 cacggattcg gatggcaatc ccgccagcgt ctccgcatcg atcaaaacgt cattcatccg
      901 atgaaggctc cgcaaaacgt ccgcggcgaa gaacccttag cgtacaccgt gggccgggat
      961 tcggccgccg cggatggctt tcgcggggga atgctgccat gccagccgct gcgcgatcac
     1021 ggcatacgcc ccatccttcg caaaagattg accagcatca acgcggtggc gccccagatc
     1081 cgcggcgcga cgccggtgta ttcatacacc ttccggggct cgccgcgcag cgggatttcg
     1141 tattggcgca gattggcggg ttcgagcagg aaatcgagcg gcacctcgaa gatccgcgcg
     1201 acttcatccg gttgcggcga gaggacggcg tcgggcgcca gcctcgccac caccggcgtg
     1261 acgcaaaagc cgctggtcgt ttccagccgg tcgaggtaac ccagcagctc gactgcaccc
     1321 gcatcgagcg cgatctcctc gcgggcttcg cgcagtgcag tcgccgtggg gtcggcatcc
     1381 ccgggatcgg accggcctcc gggaaagctg acctggcccg catggtgcgc gagatcgtcg
     1441 cagcgcagcg tgaacaccac gccgggctcg ggcgcatcgc ggatagccat caacaccgcg
     1501 gcttcggcgc gtggtgcgtc ttccaggatg ccgcggagcg atgcatggtt ccagcccggc
     1561 ggcgcgggcg gagcatcgag tggatgcaac gcttgtccaa cggccgtgaa ccagttcgtc
     1621 atggccgtgc cgccctgcgc acgggctgca cctccgcgat ccagcgccgg cgttcggcgt
     1681 catccatcgt gccccagcga gcgatttccg ccaacgtgcg acggcaaccc acgcacaacc
     1741 cttcggcatc caaccggcaa atgccgatgc acggcgtcac cggcggttgg tgttccggac
     1801 gatggggatc gggcgacatg ccacaagcct accgcacccg caatgaccgg ggcgacggca
     1861 acgtgcgtgg acaacccctg tcgcgcgcgc ctggttcgcg cggacatcga aacaacgccg
     1921 attgcgcgtg aacgaacgcg ccggaaaggc gaggcgcttg ccgccgcggt cagggctcca
     1981 cgccgagcag gcggatgtcg aacaccagcg cctcgttcgg gccgatcctg ggcggctcgc
     2041 cgcggatgcc gtaggcctgt tccggtggga tgaacacctg ccaatgatcg ccgatgtgca
     2101 tgcgggtaat gatctggcgc cagccgggaa tcatctggtt gaccggaaat accacgggct
     2161 tgccgcggtc ttcggtgttg tcgaacacgg tcccgtcgag gaatgcaccc cggtactgca
     2221 acgtcacctt gctgtcgacg gtcgggctct tgtcgccact gccaccgtcg agcaccttgt
     2281 actgcacgcc cgacgccagc gtcacgacgc ccggcttctg ctggttgcgg gcgaagaatt
     2341 cgtgtgactt ccggaggttc gcggccgcca gcttctggta ggccgcgcgc gcatcggcga
     2401 tcatctgctg ctgcatcgcg cggatcacct gctgcatttc ctgcggcgga taggcaggct
     2461 tcttgcgcga gtagccatcc tgcacgccgc gcagcaccgt ggcgatgtcg acgtcgtgct
     2521 tgcgcctgcc gagatcgagg ccggcctcgt aaccgagtgc gtaggaaagc tccgcccgct
     2581 tggccgggga cgtctccggc ttggcggttt gtgcggcggc cgcgtcgagc gcccacagcg
     2641 tgcccgcaat gagcaccatc gccacgacga ccctgcccat gccggtttcc aagacgttgt
     2701 gcgtcatccg ttgtccgcga tcaatggcac caatccgcgc tgttgcgccc atttggagcg
     2761 ctgcggccgc gaaaggttcc cgaatgtcat tgtttttccc ggtacttctt ttcgcccgcc
     2821 cggaccgcca ggtccagccg gttctccggc ggcgccagcg gacaggtcgc gtaggccgtg
     2881 aaggcgcacg gcgggttgta cgccttgttg aagtcgagca cgatcctgcc gtcgctgggc
     2941 atgtccgcat acaggaagcg cgcggcgcca tgggtttcct ttcccgacgt ggcatcgccg
     3001 aagatcagga acagttgccg atcgccaggc gcctccagca cgggaaacag ttcgaaggtc
     3061 cggccgtcgc gctcgaacac ggccttgccg ggcgcaggat accgttcgat ggtgccgatc
     3121 acggtccctg ttgcaagggc gaacggcgga tccaacgctt gccattccgc gacgatgcgc
     3181 caagccggat cgaccgggaa gcgatcgatg cccgaaaagc gggtacgcgt cgcggcctcg
     3241 ctatcgcgca cccgcagtcc cttgcggccg ccgcgctcga tggccacgaa gttaaccgtg
     3301 ccgaacgcca cctcggtggg cgcgggatgg ctgtcgtcga gcaacgccgc gtgcgccgtg
     3361 cgtcgaccgt cgaccgttgc atcggccgcg gaatcgatag tcagggacaa cgacccgtcc
     3421 gcgtgccact cgatgctgcc gagatgcgcg ggtgcctcgg cgagcacgac gtcgttgtcg
     3481 gcagcgctgc cgatccggtt cacgccgggc tgcagccatt cgaggccgac caggctcagc
     3541 cacccgcgcg gcgcggtgag ccgctcgacg cgcgccgcgt gccacgcctc gatttcgcgt
     3601 tgatacgact cggaatccat ccggcgctcc cgtttccgcc aatcacaact gccccacgat
     3661 aacggatgcc cctgctctct gttccgtgcc cacctgctcc gctcaccgcc cgcgcaggaa
     3721 cagcttgtcc agctccgcga ccgacaattg cgtccacgtc ggccgtccgt ggttgcactg
     3781 gccggagcgt tcggtcgatt ccatctcccg cagcagcgca ttcatctccg gaagggtcaa
     3841 tcgccggtgg gcgcgcaccg aaccgtggca ggccatcgtc gacagcaatt cgttctgcaa
     3901 ttcctcgagc cgccgcgaac tgccatgttc ggcgagctcg gcgagcacgt cgcgcgcgag
     3961 accgctggca tcggcgccct cgagcaacgc cgggatgcgc cgcgctgtca cctggcgcgg
     4021 accgctgcga tcgagctcga agccgcacgc cgcgagcatc gctgcgtgtt cttccgccgc
     4081 cgtggcctcg cgttcgctca ccgcgatcgc ctcgggcacc agcaattgct gcgaccgcac
     4141 ccggttggcg tcgcgggcgg tcttcagctt ttcgtaggtc acgcgctcgt gcgcggcgtg
     4201 catatcgacc agcaccaggc cgtgcgcgtt ctcggcgagg atatagatgc ccgctagctg
     4261 cgcgagcgcg tagccgagcg gtggcgcctc gccagcttcc gcctgctgca agccgacgct
     4321 cgcaggacct gagccgtggt ccgcgccgag caatgcggca tacggatcgg tgggcgcgtc
     4381 gcggatgccg aggccgtact ggcgcgcagg gaaaatgggg ccaggtacat tttccacacg
     4441 cgaacccggc agcaaccgat cttcggcaac ggaaaatgtg cctggcccca attcggcgtc
     4501 tccggcgcgg gtctgtgcca ccgcttcgtg cagcgcgtgg aacaggaaat cgtgcaccag
     4561 ccgcgtgtcg cggaaacgca cctcgcgctt ggccggatgc acgttgacgt cgactgccgc
     4621 cggatcgagc tccaggaaca gcacgaatgc cggatggcgg ccgtggtaca gcacgtcggc
     4681 ataggcctgg cgcaccgcat gcgccacgct gcggtcacgc accagcctgc cgttcacatg
     4741 gaaatactgc tggtcggcct gcgcgcgcga ggcggtcggc aaccctatcc agccggccag
     4801 ccgcaggccg gccgcagagt ggtctatccg caggcagttg gctgcgaacg cgtcgcccag
     4861 cagctcgccg acccgcgtga gctgcgccgc gtcatcggcg gcccccttga ggatgcgtac
     4921 cggcttgccg ttgtgggtga gccggaactc gacgtcgggc cgcaccaggg caagcgcacg
     4981 caccagctcc tcgatgtgcc cgaactcggt gcgctcgctg cgcaggaact tgcggcgcgc
     5041 cggcaccgca tggaacaggt cgcgcacttc caccgtcgtg ccgggcggat gcgcggatgg
     5101 ttgcggctgc gccgcccccc cgtccaggcg catcgcatgc gcgtcggaat gacggcggga
     5161 ggtgatgctc gtgcgcgaca ccgatgcgat cgaggcgagc gcctcgccac ggaagccaag
     5221 gcttgcgacg tgcgtgaggt catcgaagct cgcgatcttg ctggtcgcgt gcggcgccat
     5281 cgccagcggc agttccgcgg ccgcgattcc gccgccatcg tcgcgcaccc ggatcaagcg
     5341 catccccccc tgctcgatat cgatgtcgat gcgcgccgcg ccggcgtcga ggctgttttc
     5401 ggcgagctcc ttgaccaccg acgcgggtcg ttctatcacc tcgccggcgg cgatctggtt
     5461 gatgagttcg ggagcaagcg gacggatgga agtcatcgtg cgcaggataa cggagaggtg
     5521 gagccaggaa cctggacgcg gatcaacgcg gataaaagcg gattgttgcc agtggtctgg
     5581 ccgagcccgc atccatttgc gtcgttcccg gatgggtagt gctcgacgaa gagccccgga
     5641 gcaggctcga tgcagatgga tgcggattgc ttttttgcct tatccgcatt tatccgcgtc
     5701 aacccgtgtc aaatgctctt tcttgaaccg tggcagatca atcgatggca tcgccaccga
     5761 cgatccggta gcaggggacg tacttgctgc ccggaagctt catccggtgc tgggcgacga
     5821 agccctgcag cagctcgtcg agggcgcgca tgaacgcggg atccccatgg atctcgtagg
     5881 gtccatggcg ctcgatccgc tgcatccctt cttccttgac gttgccggcg acgatgcccg
     5941 agaacgcgcg ccgcatgtcg gccgcgagcg catgtggagc gcggccgcgg tcgaggtcga
     6001 gcgcggccat cgcttcgtgg gtcgcgacga atggttgctg gaaggacagg tcgatctcca
     6061 tctcccagtt gaaaaagaac gcgtcgtgtt gctccacgcg gtggttgcgg acgtcctcga
     6121 tgccgtgctg catcgcgtgc gcgaccgcga cgggatcgtc gatgatgatc cggtagcggc
     6181 gggcagcggc ctcgccgagc accagcttga ggaaggtgtc gatttgctcg aagtaagacg
     6241 ccgccactgc agggccggtg aacaccagcg ggaacggcag gtccgcgttg cgcgggtcca
     6301 gcaggatgcc gagcaggtac aggatctcct cggcggtacc gacgccaccc gggaacacca
     6361 cgaaaccgtg gcccgtgcgt acgaacgcct ccaaccgctt ttcgatgtcg ggcagtatca
     6421 cgaggctgtt gacgatcgga ttgggcgatt cggccgcgat gattccgggc tcggtcagcc
     6481 cgatgtagcg gttctcgtag tgccgctgct tggcgtgcgc tatggtcgca cccttcatcg
     6541 gtcccttcat cgccccggcg ccgcagccgg tgcagatgtc catgccgcgc aggccaagct
     6601 ggtagccgac cgccttgctg tagtcgtatt cctcccgcga gatggaatgg ccgccccagc
     6661 agacgacgag attcggatcg aaacgcgcgc gcagcacccg cgcgttgcgc aggatatcga
     6721 acaccgcgtg ggtgatgccg ctggagtccg cgaggtcgaa ttgcccggct tcgatctgcg
     6781 tcgcaacgta cacgatgtcg cgaaccaccg cgaacagcag ttcgttggtg ccgcggatga
     6841 tcttgccgtc aacgaacgcc tgcgccgggg cgttcctgag gtcgagcttc accccgcggt
     6901 cctgctgcag cacctggatg tcgaaatccg gataccggtc gagcgtcgca cgcgcgtcat
     6961 cctgcaccac cccggaggtc agcaccgcca gcgtgcatga gcgcagcaag ccgtgcagcc
     7021 ccgacgccgt ggcatcgcgc aaccgcgcga tctcgttgcg ggacaagacg tcgagcgtgc
     7081 cggcgggaac gatctgtgca tcgaccgtcg cgggtctcgc gcgtcgtggc gttgcgacat
     7141 cctgcatgtt caatcgattc atccgaagca gctgttgtgg aaagtcagag cctgcccccg
     7201 aagtgcttct gatcgggggc catggatggc cgttctggtg tccgcgatcc gaaggacctg
     7261 gagccaatgc tcatcccagt gaagcagcga tcacggatga tcgcactaga tacccttcga
     7321 cgccgcccag gatcatctgc gccgacaagg cgatcagcag catgcccatc acccgctcca
     7381 cggctgccag cacgctctct cccaggaact tgaagagcca tgtcgagccg agcaggatca
     7441 ccgcggtggc aagccaggcg atcaacagcg ccacgcccca ttcgacttgt cggcccggtt
     7501 gcgcgctggt cagcagcagc agtgtcgcca tcgcggaggg tccggcgaca ccgggaatcg
     7561 ccaggggcac gatgaacggc tcgccctggc ccaccggacc gaagatgccg ccctcccggg
     7621 gcggaaacac catcttgatc gcgatcagga acagaacgac tccgccgccg atgctgatgg
     7681 cttcctggcg cagttgcatg atcgacagca actcgcggcc gccgagcagg aacgcgacca
     7741 gcaccgcgag cgcgatcagc aattcgcgca ccatgaccca gcgccggcgc ctcggcggga
     7801 cgtccttcag gatcgacagg aagaaaggca cgttgcccgg cgcgtcgagc accaggaaca
     7861 gcgtgatgca tgccgacacc atggtcatct gcgtctgcat ggctagcccc cgtgcggttc
     7921 gggtacacgc aggtccaaca gcttgccgcg cacggcgagc ccggcaggtg acagcagata
     7981 cagcacggcg cgtgccgcgg cgtcgggcgt cgccacctcg gccggatcct cgcccgccca
     8041 gacggtgcga cgcaatgccg tgcgcatcgg cgccggcagc aaggcgtgca cgcgcagcga
     8101 ggtcgaatcg gtctcgtcgt ggaggatcga ggcgagccgc tccagccccg ccttggccac
     8161 cccataggcg ccccagtgtg cccgccccat ccgttccgga tcgtccagca cgaataccac
     8221 ggcgctgtcc tcccgcgcca gcaacagcgg catgcaggcc tgggtcagaa ggaacggtgc
     8281 ggacaggtcg acgtgcaacg tcgtcatcca ttcgtccggc gacagcatcg cgagcggggt
     8341 gaggccctcg catttcaccg cggcgtggac cagcccgtcg agccggccca gctggcgctc
     8401 gatcgattcc gcgaaggtct ggtattcgag cggcgaggcg ccctgcaggt ccaccggctg
     8461 cagcaccggc tggggcgcac ccggcagtgc cgcgatttcg tcgtagaccg gttccagcgc
     8521 gcgcgtcttg cggtcggcca ggactaccgt cgcgcccgct tgcgcgcagg ccagcgccgc
     8581 tgcccggccg aggccgcccg cagcgccggt gatcagcaca atccgttccg ccagcgcgtc
     8641 cgcggcgggt tcccaatcca ccggcggtcg cgcgacgggc aggctcatcg ctcggtgggc
     8701 tgcttcgttg cctcgccgac catccgctgc aattcaccgg actcgtaaag ttccagcgtg
     8761 atgtcgcagc caccgatcag ctcgccgttg atgtagagct gcgggaaagt cggccagttg
     8821 gagtagcgcg gcaggttggc gcggatttcg gggtcttcca gcacgttcac cgagaaatac
     8881 tcggcaccgg tggcattcag cgcctgcgag gtgcgcatcg agaaaccgca catcgggaag
     8941 tcgggcgtgc ccttcatgta cagcacgatg gagtgctcgt cgagcgtgtg cttgatgcgt
     9001 tcgattacgt ccatgggata tgggtctaga atggagggtg gtgtcatgcg gacgccgcgt
     9061 attgtaccag cgcgcgaacc gggcatgcct ccacatccgc ccttcgaata aaaggagaat
     9121 cccatggcga tcgaacttcc cccgcttccc tacgagcgca acgcactggc gccccacatt
     9181 tcggaggaga cgctggagta ccaccacgac aagcaccaca aggcctacgt cgacaacttg
     9241 aacaagcaga tcgagggcac cgatttcgcc gacatgaacc tcgaggacat cgtcaagaag
     9301 gcgaccggcg gcatgttcaa caatgccgga caggcgtgga accacaactt ctactggaac
     9361 tgcctatcgc cgaatggcgg cggcgaaccc tccggcaagc tcgccgacgc catcaagaag
     9421 gccttcggcg acatcggcaa gttcaaggag gaattcgcca agatcgcgac cggcacgttc
     9481 gggtccggct gggcgtggct ggtgcagcgc accgacggct cgctgggcct cgtcagcacc
     9541 cccaacgccg gcaccccgat caccggcagc gaccgcccgc tgctgacctg tgacgtctgg
     9601 gaacacgcct actacatcga ctaccgcaac gcgcgcgcga agtacgtcga tgcgtactgg
     9661 agcgtcgtga actgggactt cgccacgacc cagatggcgt agcacgcgcc cgcccggagc
     9721 gctccgcagg cgaaagccac ggggcgtttc tttcttgcgc cggtccgtta cgccgcggcc
     9781 gcgctttcga cggcggtcgc cagtttcgag gccagcgcct gcacctcgtc cgcgtcctcg
     9841 ccctcgatgg tcacgcggac cagtggctcg gttcccgaag ggcgcagcac cacgcggccg
     9901 cgtccgtgca ggaccacctg cacttcctcc agcgcatgct gcacggcagg ctgggccacg
     9961 agttggcgac cgccgctcac ccgtacattg cgggtgattt gcggcagctt cttcaggtcc
    10021 gcgcgggctt cggaaagatc gaggccgctg cgcttcaccg catcaaggac cgcaagcgcc
    10081 gagacgatgc cgtccccggt gctggctcgg tccaggcaca ggatgtgccc cgaagtttcc
    10141 ccgccgagca ccccgtggtg ctgcttcaac tgctgcagga cgtagcggtc gccgacattg
    10201 gcgcgcagga acttgacgcc ggcgccggtc agcgcgcgct ccagtccgta gttgctcatc
    10261 aaggtaccca ccaccggccc ccgcaggtgg ccgtcctcca gccagtcgcg cgccagcacg
    10321 tacaacaggt cgtcgccgtc ggcgagttcg ccgttgcggt cgatcatctg cacgcggtca
    10381 ccgtcgccgt caaaggcgat gccgaggtcc gcaccttgtt cgatcacggc gccgcgcagc
    10441 gcgaccggat gcgtggaacc gacttcacga ttgatgttga agccgtcggg gtcgcagccg
    10501 atgcgcgtga tcctcgcacc cagcgattcg aacaccttgg gcgccacggc gtaggtcgcg
    10561 ccatgcgcgc agtccagcac gatgtgcagg cccgacaggt ccggcggcgc ctgcagcgtg
    10621 ccgaggcaaa agcgcgcgta gcgctccacc gcatcgtcga tccggcgggc cttgccgagc
    10681 ccttccggcg cgaccgtcgt gaacggcgcg tcgatctcgc gctcgatttc gcgctccacg
    10741 tcgtcgtcga gcttttcacc cgtcgccgag aagaacttga tgccgttgtc ctggtagggg
    10801 ttgtgcgagg cgctgatcac gatgccggca ctggcgccca gcgcgcgcgt caattgcgcg
    10861 accgccggcg ttggcaacgg gccgagcagc ccgacgtttg cgcccgccgc caccaggccc
    10921 gattccagtg cggcttcgaa catgtatccc gacacacggg tgtccttgcc gatcaacacc
    10981 agcggacgct tgccgttgcc cagcaccacc cccgcggcgc gccccaattt cagcatgaac
    11041 tcggcggtga tcggccattc gccgacgcgt ccgcggacgc c
//
LOCUS       DASSNH010000245        26929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1788861, whole genome shotgun sequence.
ACCESSION   DASSNH010000245 DASSNH010000000
VERSION     DASSNH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 26929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26929
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788861"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFK00_08990"
     CDS             complement(<1..179)
                     /locus_tag="VFK00_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET7593124.1"
                     /translation="MKEPLRYTRLSGLEPLVITPETLFVNIGERTNVTGSAKFKKLIK
                     EDRYDEAVEVARQQV"
     gene            complement(311..1414)
                     /locus_tag="VFK00_08995"
     CDS             complement(311..1414)
                     /locus_tag="VFK00_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7593125.1"
                     /translation="MAHTLPYLHPDRVDALHSALARRILILDGAMGTMLQRHELDEAG
                     YRGERFATGCDAWHAHGHAGEHAGPGGCQRDLKGDNDLLSLTHPEIIRDIHAAYLDAG
                     ADLVETNTFNSTSISQADYALQHLVPELNREGAKLARAACDEAERNTPGHPRFAIGVL
                     GPTSRTASLSPDVNNPGFRNVDFDELEAAYADATRGLFEGGADIVMVETIFDTLNAKA
                     ALSGISRVFDEVGARLPIMISGTITDRSGRTLSGQTAEAFWYSVAHARPLSVGLNCAL
                     GAKDLRQHIEALSRVADCCISAHPNAGLPNELGGYDETPEEMAKTLGEFARDGLLNIV
                     GGCCGTTPEHIEAIAEAVRGVPPRVLPSHAQAA"
     gene            complement(1500..2432)
                     /locus_tag="VFK00_09000"
     CDS             complement(1500..2432)
                     /locus_tag="VFK00_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7593126.1"
                     /translation="MLDLVTATALLRVLSDPTRVRLLALLRREELTVAELSSVLRLAQ
                     PRVSTHLAKLKESGLVHDRRAGVSAYYRFNEHGFQNAADLLRALEDSVDDAVLDGDAQ
                     RLPAVLTARARTRGWADSVAGDMERHYSPGRTWETLARTLLQLLETGDVLDIASGDGV
                     LAELLAPHAHSIVCVDSSANVVAAARKRLKVFRNVEVVEGDMHALELGERRFDLVLMM
                     HALTYSGQPQQAVAEAARVLKRGGRLLAATLAKHAHRAAVAPFDHKNLGFKREELSGF
                     ARKAGLEVLQCERISREARSPHFEVLSLLARKPG"
     gene            2580..3740
                     /locus_tag="VFK00_09005"
     CDS             2580..3740
                     /locus_tag="VFK00_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7593127.1"
                     /translation="MDFQFSEEQLQIQSIARDFAQKRIAPVAADFDKSGEFPLENIRE
                     MGKLGLMGIEVPAEYGGAGMDSISYVLAMIEVAAADCAHSTIMSVNNTLYCNGLLKFG
                     SEEQKHKYVTPIASGGAIGAFALTEPQSGSDATAMRCRAARQDDGSFVINGKKSWITS
                     GPVASYILLFAMTDPGKQARGITAFMVDTSRKGFSRGKTEPKLGIRASATCEIEFDDY
                     IAQPDEVIGEEGHGFKIAMGVLDAGRIGIASQAVGLARAAYEATLEYSRDRKAFGHPI
                     GSFQMTQAKIADMRCKLDAARVLTLRAAWLKQESAKTGARFSTEAAVAKLTASEAAMW
                     ITHQAVQIHGGMGYSREMPLERYFRDAKITEIYEGTSEIQRMVIARNETGLR"
     gene            complement(3862..4698)
                     /locus_tag="VFK00_09010"
     CDS             complement(3862..4698)
                     /locus_tag="VFK00_09010"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HET7593128.1"
                     /translation="MAIYAIGDVQGCHPELQRLLDRLRFDPASDRLWFCGDLVNRGNG
                     SLEVLRLVHGLREHCVVALGNHDLSLLAIAEREPEARARTNQDLRRVLEAEDGPVLID
                     WLRGQSLLHHDATLGFTLVHAGLAPSWTLKQAQHIAHEVERALRGPGHHALLQRMFGN
                     RPARWSSRLHGHDRLRAAINIFTRMRYCDAHGRIDFDAKGPPGSQRTGLYPWFEVPGV
                     RTRDTRIVCGHWSALGRFAGLGIYAIDTGCVWGGKLTALRLDREDPEFISVDAEPHRT
                     RK"
     gene            complement(4717..5139)
                     /gene="apaG"
                     /locus_tag="VFK00_09015"
     CDS             complement(4717..5139)
                     /gene="apaG"
                     /locus_tag="VFK00_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET7593129.1"
                     /translation="MSPRQTSTDQPRDAREPEPYSVEVEVEASFVAEQSKPEADRYVF
                     AYEITLRNLGVMPVRLQTRHWVITDANGKVEEVHGEGVVGEQPRLQPGEHYRYTSGAV
                     LETQVGTMRGAYLFHADDGTDFEVPIPEFVLSVPRTLH"
     gene            complement(5152..5976)
                     /gene="rsmA"
                     /locus_tag="VFK00_09020"
     CDS             complement(5152..5976)
                     /gene="rsmA"
                     /locus_tag="VFK00_09020"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7593130.1"
                     /translation="MPRPKKHFGQHFLHDRSVIERIVAAIAPKPGDRIVEIGPGEGAL
                     TLPLLRAAGRLTAIELDRELIEPLRARAAGVGELEVIQADVLKTDLGALAGAARLRLA
                     GNLPYYVSSPILFHCLDFADAIADMHFMLQKEVVERMAAQPGGKVYGRLSVMLQLACR
                     VEPLLRVAPSAFRPPPKVDSAVVRLVPLPAAKRPDAPIALAIENVVRAAFGQRRKTLA
                     NALRGLADPDLIRAAGIDPGVRAENVPPQGFAALAREVASANLQGGTKLADNAGAP"
     gene            complement(6152..7192)
                     /gene="pdxA"
                     /locus_tag="VFK00_09025"
     CDS             complement(6152..7192)
                     /gene="pdxA"
                     /locus_tag="VFK00_09025"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7593131.1"
                     /translation="MNGEPVATGSHPPALRKLAVTGGDPAGVGPELVARLAATPIPAD
                     LIAIGDQSLFERAAHRCGVALKIEAYDGRPRTMRAAGMLRVLDVPLRCEEVPGRLDPR
                     NAFYVLATLARAADGCMAGEFDSLVTAPVQKSVINDAGERFSGHTELFAQRAGRDVVM
                     MLASPELRVALATTHLPLREVPAAITPERLVRTLRIVHAELIGKFGIDGPRIAVLGLN
                     PHAGEGGHLGHEEIDTIVPAMDTLRGEGMRLLGPLPADTAFVPAARAGYDAVLAMYHD
                     QALPVLKSEAFERTVNITLGLPFVRTSVDHGTALDLAGTGRADPSSMFAAADVALQLA
                     ARKQESIDAAPI"
     gene            complement(7189..8607)
                     /locus_tag="VFK00_09030"
     CDS             complement(7189..8607)
                     /locus_tag="VFK00_09030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7593132.1"
                     /translation="MTRSRFFARLALSFAACAFALPAFAQLLSPNAPPPTGTQDSSLL
                     APQAAAPNGVQPLDRIVAVVNDGVILQSQLDQTMATVAQQISASGGRQPPREVLEKQV
                     LQRLILNELLVQKAQENGLRIPDAQVDAAVANIAKQNNMTVPQMQAAMQQQGIDYASF
                     RDQLRDQFLVRQVQQQVMESNGQVSDAEIKNLIASPAFKKGEVHLARILVGLPEGADA
                     NQIAAAKAKAEQIEGELKDGKGFAALAVSNSSAPEALEGGDLGWRRVDELSPAVQQVV
                     ESLQPGDHTQPLRDASGFTILRLEGRRTPDTKQIVTEYHARHLMIKPTAVLSEQEAKD
                     KINKLYQEIVDGHADFAKLAKQESDDPTTANAGGDMGWFMQDDWGSDVGKLLAGMQPG
                     QVSQPFQSHDGSWHIIELLGERQADKTRDLERQQARQAIAARKGQQAYEQFLRDLESS
                     AYISIRLPELAGASPAAADQGQ"
     gene            complement(8877..11150)
                     /gene="lptD"
                     /locus_tag="VFK00_09035"
     CDS             complement(8877..11150)
                     /gene="lptD"
                     /locus_tag="VFK00_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HET7593133.1"
                     /translation="MLRRIKPPTPRALAFAITLAAAPGMALAQTGPRVCPLGAFVCPV
                     ATNDFALCKKNDLLDFYQPGLPTTGDREASPAQVFGDTFSSPDSNLFHLSGHTSLQRL
                     DQLLRADNLTYNRETTAYDAEGDVRYQERGMLFSADSMKGTTTPEYGVADNVQYQLLD
                     SHGNGVASRAIMLDQDRDKLHDVTYSTCELHDRVWEIEAKTITMDRDEGLGRAHDVTM
                     RLKGVPFLWLPYMRFPLDDRRQTGFLFPSFGKRSRAGYFFSIPFYWNIAPNYDATFTP
                     TWYAERGAMLDSQFRWLTSTSKGELDLDYIPHDKVTDTDRTHLILANQTRLPWDLRWS
                     TRINWISDKAWYQDYGENFRTSNLRQLHSSSYVNGSGDWWNAGAGADFYQIADPSLSD
                     RSAAYRRLPRVYFNAGGPIGGTGGPEWGTETEAVHFVKPDEIGGDRFDFYPYLAWPLQ
                     GAAWFLRPELGLRYTTYNLPRPLVKGYSTHPSRTTPIFDLDAGLVFERDVHLFGQEYT
                     QTLEPRAYYLRVPYRDQDDIPLFDTRQMTFDFWQLFTTNTFSGADRQMNANNLSLALT
                     TRFLDENGVEKLSAGIGQIRYFEPQRVTLRRGGKEVDYAGSNYVANLTLSLSDNWRLT
                     ASQQWNPNTNQTDVSTVGLQRRLWGDGIVNLSYRYRRNLLDQADVSAEVPVGAAWKLV
                     AGYNYSFRDQMALDAFAGVEWDSCCTAIRILSRHYVYDYQGHSDDAIMFEIQFKGLGS
                     YGQHLGSFLQTAILGYQ"
     gene            complement(11225..13042)
                     /locus_tag="VFK00_09040"
     CDS             complement(11225..13042)
                     /locus_tag="VFK00_09040"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET7593134.1"
                     /translation="MTDANIPQRIAALRHVMHERGIAACIVPTADPHLSEYLPARWQA
                     REWLSGFTGSAGTLVVSRDFAGLWTDSRYFSQAERQLAGSGIELMKLNVPHTAEHVAW
                     LCGRLDEGDKVACAADMLSLAAEHSLREQFSAHGGELVEDDLPGAIWSGRPPLPHAPV
                     YEHPIDYAIRSRAEKLGDVREAMRASGASHHVVSALDEIAWVLNLRGSDVDYNPVFLA
                     HLLIGAGEATVFVDAAKLDAKLRSRLADEGVRVAPYEAASDALRELSKDTRLLLAPGQ
                     VSAALAHAIPEIVVVIEAPGPITAAKARKSDKEMEHVREAMRRDGVALVRGARWLEQS
                     LKDGRRLTELDVDEKLRGLRSQQPGFVSESFSTIAGYEANAALPHYSATPESHAELRA
                     RGMLLIDSGAQYLGGTTDITRMWALGETTPEQRRDVTLVLKGVIALSRAKFPRGASGQ
                     QLDALARAPIWAAGIDYGHGTGHGVGYCLNVHEGPQSIRPPRSASQLEPMDVGMITSI
                     EPGIYKPGRHGVRIENLAATVSAGDGEFGEFLAFETLTLCPIDTRLLDMALLDPAETA
                     WLDGYHAMVRERLAPLLDDPADRAWLDARCAPLSTARAA"
     gene            13166..14833
                     /locus_tag="VFK00_09045"
     CDS             13166..14833
                     /locus_tag="VFK00_09045"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7593135.1"
                     /translation="MTSLIFVTGGVVSSLGKGIASASLASILEARGLNVTLMKLDPYI
                     NVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFVRTRLSGKNSITTGKIYEAVIRKE
                     RRGDYLGATVQVIPHITNQIKHAIDEATRGFDVALVEIGGTVGDIESQPFLEAARQLR
                     IERGADNVLFMHLTLVPFIKAAGEIKTKPTQHSVKELRALGVQPDILVCRCEEPLPES
                     ERRKIALFTNVPESAVISAADADTIYKLPLWLHKEGLDDIVVERLHLDAKPTDLAEWR
                     RTVDAVENPRDEVRVYIVGKYVEHKDAYKSLGEALRHGGIHNQTRVNLEWVDSEIIET
                     RGADAVLHDADAILVPGGFGKRGFEGKIQTVRYARERGIPYFGICYGMHAAVVEFARD
                     VAGLSGAGSTENDRDCLEPVIALITEWTQAAGDVQHRDETSDLGGTMRLGAQECRLKP
                     GSLAHELYGADTIRERHRHRYEFNNRYRERLEKAGLVIAGVSVDDLVEIVELPQQKHP
                     WFLACQFHPEFTSTPRDGHPLFNGFVRAAREYKAMRDAPRLAKEAAG"
     gene            14830..15663
                     /gene="kdsA"
                     /locus_tag="VFK00_09050"
     CDS             14830..15663
                     /gene="kdsA"
                     /locus_tag="VFK00_09050"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET7593136.1"
                     /translation="MNLCGFDVGLDQPFFLIAGPCVVESEQLQIDTAGALKDITGRLG
                     VNFIFKSSFDKANRTSGKSFRGLGMEEGLRILGEVKKQLGVPVLTDVHEYTPFDEVAA
                     VVDVLQTPAFLCRQTDFIQKVAAAGRPVNIKKGQFLSPWDMKNVVEKARAVGNDQILV
                     CERGASFGYNNLVSDMRSLAVMRETHCPVVFDATHSVQLPGGQGTKSGGQREFVPVLA
                     RAAVAVGVAGVFMETHPDPDEALSDGPNAWPLDKMEALLTTLKELDAVTKHNGFLEMR
                     S"
     gene            15761..17059
                     /gene="eno"
                     /locus_tag="VFK00_09055"
     CDS             15761..17059
                     /gene="eno"
                     /locus_tag="VFK00_09055"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7593137.1"
                     /translation="MTTAIKTIHAREVLDSRGNPTLEAEVTLGDGAFGRAIVPSGAST
                     GTREAVELRDGDKARYLGKGVTRAVGNVNGEVAKALAGFDAADQKGLDEKLIALDGTP
                     NKSRLGANATLGVSLAAAHAVAASRRQPLWKYLAGDRAPMLPVPMMNVINGGVHADNP
                     LDMQEFMVMPAGFGKFSDALRAGVEVFHALKTQLKKRGMNTAVGDEGGFAPALRSHEE
                     AIEVILEAVNAAGYTAGEQVWIALDPASSEFFDHGVYDLKGENRKLDAAGMVGLYADW
                     CGKYPIISIEDGLAEGDQAGWKLITETLGQKIQLVGDDLFVTNPSIFREGIANHIANA
                     ILVKVNQIGTLSETLEAVAMADQAAYAAVISHRSGETEDTTIADLAVATTSTQIKTGS
                     LCRSDRIAKYNQLLRIEEQLGSGAKYAGRDAFPNLKALPG"
     gene            17072..17410
                     /gene="ftsB"
                     /locus_tag="VFK00_09060"
     CDS             17072..17410
                     /gene="ftsB"
                     /locus_tag="VFK00_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HET7593138.1"
                     /translation="MLRWIALVLIALLIGLQVKLWVGDGGMRDLRAIRARVSSQQAEN
                     AKLKQRNEALGADVEDLKHGQDAVEARARSQLGLIKPGEVFYQVVVAPSGSSAAASPA
                     AASTAAASAH"
     gene            17490..18284
                     /gene="surE"
                     /locus_tag="VFK00_09065"
     CDS             17490..18284
                     /gene="surE"
                     /locus_tag="VFK00_09065"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7593139.1"
                     /translation="MRMLLSNDDGVDAPGLGVLAERLAEVGEVTVVAPDRDRSGASNS
                     LTLDQPVRVARLDERRYRVVGTPTDCVHLALSGLLDGEPDIVVSGINNSANLGDDVIY
                     SGTVSAAMEGRFLGLPAIAVSLAGIGERHDGNGHHFDSAARAVLLLMRRLLVDPLPAD
                     TILNVNVPDCPWSAIRGFEVTRLGRRLRSEPCVPLKDPRGRTLWWIGAAGEADDAGPG
                     TDFDAVRRGFVSVTPIHVDLTRFQALEKVSSWIGGLTAQMEGEQAA"
     gene            18281..18961
                     /locus_tag="VFK00_09070"
     CDS             18281..18961
                     /locus_tag="VFK00_09070"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET7593140.1"
                     /translation="MNTLFGAVAASAGGRGMTSQRARDRLVATLRGEGISDPRVLDVI
                     RTTPRHLFIDEALASRAYENTALPIGLGQTISQPWVVARMTELLIERAIPDRVLEVGT
                     GSGYQAAVLSQLVGTVYTVERIEELLRKTRRLFRTMKLENIRTRHDDGNIGWTAEAPF
                     DAIVLTAAGETVPRALCEQLAEGGVLVAPLGPAGSQRLTRISREGESWPREEFEAVSF
                     VPLLGGLG"
     gene            19020..19622
                     /locus_tag="VFK00_09075"
     CDS             19020..19622
                     /locus_tag="VFK00_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HET7593141.1"
                     /translation="MKVFRPLYEWALKWAAKPRADAWLCVLSFFEAFIFPVAPEIMLA
                     PMTLARPKRWWYFATISLAGSLVGSMIGYELGHYAFHYLQPFLADIGWSQHLDAMVEK
                     FGTAAKRHPWEAFWMLVAAGFLPIPMKVASWACGIVGMPILPFLAGILIGRGKRVFLV
                     AGVIRLGGERAEKALHKHIETAGWVIVAIVVVLGLYFYLR"
     gene            19797..20858
                     /locus_tag="VFK00_09080"
     CDS             19797..20858
                     /locus_tag="VFK00_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7593142.1"
                     /translation="MKVLFAAVASAALLAGCASTPAPVVNHSFRPGAPASPGAPASQP
                     AASNAAGTQTFGLGHANGTVSAAGYTVAPGDTLYSVAFAHGMDVRTLAAQNEIPPPYV
                     IRPGQVLKLEAPQAAGATAAAGTNAIAPPPAPATQSTSPVFGPVTTQSMAANGVAPAA
                     SPPAASTAPVFGPVTTQQMTPGGVASASSSSAVVVAESPPAAANVPPVSAAPVPAMTP
                     TPPPGTTRTAAGITWRWPASGKVVGGFKAGVGGDGAGVDIAGNVGDPVRAAADGTVVY
                     SGNGLIGYGELVIIKHNDTYLSAYGHNSRRLVKEGEHVDAGQEIALMGASGAPRVELH
                     FEIRKDGKPVDPLGYLPDR"
     gene            complement(20982..21362)
                     /locus_tag="VFK00_09085"
     CDS             complement(20982..21362)
                     /locus_tag="VFK00_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HET7593143.1"
                     /translation="MELNLERPDDFLFVRKADAHSVIVGDRAFTRSLILTRTQVIDDW
                     GIAHVAEITPGHIEPIVALKPDVVLLGTGARQQFPPQEVLAAFLQRGIGLEAMDNAAA
                     ARTWDILAGEGRNVVAAFVLCDRP"
     gene            complement(21369..21662)
                     /gene="yhbY"
                     /locus_tag="VFK00_09090"
     CDS             complement(21369..21662)
                     /gene="yhbY"
                     /locus_tag="VFK00_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET7593144.1"
                     /translation="MLTASQTRYLRGLAHPLKPVVLLGGKGVTPSLLTELEQALADHE
                     LIKVRLSGGDRAARAAEVMKLAESSHAETVQTIGHVAVLYRRNDEQPKIALPK"
     gene            21739..22359
                     /gene="rlmE"
                     /locus_tag="VFK00_09095"
     CDS             21739..22359
                     /gene="rlmE"
                     /locus_tag="VFK00_09095"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HET7593145.1"
                     /translation="MARSKSSARWLKEHFGDEYVKRAQAEGLRSRAAFKLDELLERDH
                     LLKPGMVVVDLGAAPGGWSQLVRQRLGDNGRVFALDILPMQGIGGVEFLEGDFREQRV
                     FDALMERLAGSQVDLVLSDMAPNISGIQAADQARAMELAELASDFAGKCLKPGGSFLV
                     KLFQGRGFDEYIRGLRAGFGRVTLRKPKASRARSREVYALATGRKS"
     gene            22461..24398
                     /gene="ftsH"
                     /locus_tag="VFK00_09100"
     CDS             22461..24398
                     /gene="ftsH"
                     /locus_tag="VFK00_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7593146.1"
                     /translation="MNDLTKNILLWLVVAVIVVLVYQSLSPRMSGGEQEMSYSEFVQQ
                     VKNDNVSSITISPNMPPVITGKLKDGNDLRTVLPAVGDNSLLPLLQSHRVKTTQKPGD
                     NGFSLMRLIIEWLPFIVVFGLLFYIFRQMQSGAGGRGAMSFGRSRAKLQGEDQIKVTF
                     NDVAGCEEAKEEVSELVDFLRDPSKFTKLGGKIPRGVLMVGSPGTGKTLLAKAIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGG
                     HDEREQTLNQLLVEMDGFEGSEGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDLK
                     GREQILKVHMRKVPAGADVDPLTIARGTPGFSGADLANLVNEAALFAARENARDVRMD
                     FFERAKDKIMMGSERRSMIMSEDEKKLTAYHESGHAIVGLSVPDHDPVHKVTIIPRGR
                     ALGVTMFLPEQDRYSHSKLSLESKLASLFGGRVAEELIFGSEKVTTGASNDIQRATAL
                     ARDMVTKYGLSTELGPMTYTEDEDEVFLGRSVTQHKNVSDETSRKIDVAVRAVIDAAY
                     SRARQILTGDITKLHAMAAALLQYETIDREQIAAIMEGREPGPPKDWTPRGDNTGMGG
                     GKPATAPGVAPAPSSHPAGQH"
     gene            24578..26176
                     /locus_tag="VFK00_09105"
     CDS             24578..26176
                     /locus_tag="VFK00_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7593147.1"
                     /translation="MLAKLKLTFSEFRRRRVFRVAAAYVVAGWLLIQLGSATFEPMGL
                     PDWSMRFLLVLLVLGFVLACLLAWVYDYGAHGLERTLPARVPLPASDGRHAAAPDASV
                     AVLPFADLSEAHDQGYFCDGLAEEILNALASIKDLRVASRTSSFRFRNRDADAREIGH
                     ALNVAAIMEGSVRKAGDRVRVTAQLIDAANGYHLWSENFDRKLEDIFAIQEEIGRNVA
                     RALRVSLNGAAKLDPGCTCNAPRDMRAYEFYLRGRQMQSLKSSDNWTKASEMFRRAIE
                     LDPDYAHAHAGLADSLAQLLIWRILQPERVLDEAVASAKRALELAPNLAEAHVARANT
                     LSLAGDNQGAVAAFRRSIELDPELYEAHYYFGRHCYARGDYVLAIEEFEAAHRVRPDE
                     FQALSLASNAADSLGDTGRGDELIRLALPPALAEIARDPENGRALYLAAGMQVRLGDI
                     EGGRRNVEAALRLQPDDFGTLYNAACTYTHMGDTDRAFDLLERAISVGHGFRDWIEHD
                     SDLDSLRGLPRYGEILARLRNPGD"
     gene            26186..>26929
                     /gene="folP"
                     /locus_tag="VFK00_09110"
     CDS             26186..>26929
                     /gene="folP"
                     /locus_tag="VFK00_09110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7593148.1"
                     /translation="MFDTTPLLDCNGRRLELDHPRVCGILNLTDDSFSGDGLAGDVGA
                     AVDRGVAMVEQGAALLDVGAESTRPGAEPVPDADELARVLPVIEALARRVDVPISIDT
                     SKPDVMRAAVGAGAGLINDVYALRCEGALEAAATLKVPVCLMHMQGEPRTMQEDPHYD
                     DVVGEVHRFLAERVFACEMAGIDKRRVLVDPGFGFGKTLDHNLALLRGLERFRDLAAG
                     VFVGLSRKAMIGTLTGRGRGVERAAGSAAA"
BASE COUNT         4421 a         8619 c         9319 g         4570 t
ORIGIN      
        1 tcgacctgct ggcgcgcgac ttccaccgcc tcgtcgtagc ggtcttcctt gatcagcttc
       61 ttgaacttcg ccgagccggt gacgttggtg cgctcgccga tgttgacgaa cagcgtctcg
      121 ggcgtgatca ccagcggctc gaggccggag agtcgtgtgt agcgcaacgg ttccttcatt
      181 tcatccttct ttggaagaac agaagctttt gacgcggata agagcggatc aaatcggata
      241 aaaccccgat tgtttgctct atccgctctt gtccgcgttg atccgcgtca aaagcctttg
      301 ctgccgaggc tcacgcggcc tgggcgtgcg atggcaatac acgcggcggc acgccgcgca
      361 cggcttcggc gatcgcctcg atgtgttcgg gcgtggtgcc gcagcagccg ccgacgatgt
      421 tgagcagccc gtcccgcgcg aattccccga gggtcttcgc catttcctcg ggtgtttcgt
      481 cgtagccgcc cagttcattc ggcagcccgg cgttgggatg cgcgctgatg cagcaatcgg
      541 ccacccgcga cagcgcttcg atgtgctggc gcaggtcctt cgcacccagc gcgcagttga
      601 gcccgacgga aagcggccgg gcgtgtgcga ccgaatacca gaatgcctcg gcggtttggc
      661 cggagagcgt gcgtcccgag cggtcggtga tggtgcctga aatcatgatc gggagtcgcg
      721 cgccgacctc gtcgaacacg cgcgaaatgc ccgacagtgc ggccttcgcg ttcaaggtat
      781 cgaatatcgt ctcgaccatc acgatgtcgg cgccgccttc gaagaggccg cgtgtggcat
      841 cggcataagc ggcttcgagt tcgtcgaaat cgacattgcg gaagccggga ttgttgacgt
      901 cgggcgacag cgacgcggtg cggctggtcg gtcccagcac cccgatcgcg aaccgcgggt
      961 ggcccggcgt gttgcgctcg gcttcgtcgc acgcggcgcg cgccagcttc gcgccctcgc
     1021 ggttcaattc gggcaccagg tgctgcagcg cgtaatcggc ctggctgatc gaggtcgaat
     1081 tgaaggtgtt ggtctcgacg aggtccgcgc cggcatcgag atacgccgcg tggatgtcgc
     1141 ggatgatctc cgggtgggtc agcgacagca ggtcgttgtc gcccttgaga tcgcgctggc
     1201 aaccaccggg accggcgtgt tcacccgcgt ggccgtgcgc gtgccaggca tcacagcccg
     1261 ttgcgaaccg ctcaccccga taaccggctt cgtccaactc gtgacgctgc agcatcgtgc
     1321 ccatcgcgcc gtcgaggatc aggatgcgcc gggcaagcgc ggagtgaagt gcgtccacgc
     1381 ggtcggggtg caggtagggc aaggtgtgtg ccatggattt ctcctgtagg tgcgggccat
     1441 gcccgcgagg gcgcccgccg tggtgtgccg gatcgcgcac atggcgcgcc tgcatccgtt
     1501 tacccgggtt ttcgtgccag caggctcaac acctcgaaat gcggcgagcg ggcctcgcgt
     1561 gaaatgcgtt cgcactgcag gacttcgagg ccggccttgc gtgcgaagcc ggacagctct
     1621 tcgcgcttga agcctaggtt cttgtggtcg aatggcgcca ccgccgcgcg atgcgcgtgt
     1681 ttcgcgaggg tcgcggcaag cagccggccg ccccgcttca gtacgcgcgc cgcctcggcg
     1741 acggcctgtt ggggctgccc actgtaggtc agggcgtgca tcatcagcac gaggtcgaac
     1801 cggcgctcgc cgagttcgag tgcgtgcatg tcgccttcga ccacctcgac gttgcggaaa
     1861 accttgaggc gcttgcgcgc ggcggcgacc acattggcgc tggagtccac gcacacgatc
     1921 gagtgcgcgt gcggtgccag caactctgcc agcacgccgt cgccggaggc gatgtccagc
     1981 acgtcgccgg tttcgagcag ttgcagaagg gtccgcgcga gcgtctccca ggtgcgcccc
     2041 ggcgagtaat gccgctccat gtccccggcg acgctgtcgg cccaaccacg ggtgcgcgcg
     2101 cgcgcggtga ggacagccgg caggcgctgg gcgtcgccgt cgagcaccgc gtcgtcgacg
     2161 ctgtcctcga gcgcgcgcag caggtccgcg gcattctgga acccgtgttc gttgaagcgg
     2221 tagtacgccg atacgccggc gcgacggtcg tggaccaggc cggattcctt caacttcgcc
     2281 agatgcgtcg agacccgcgg ctgcgcgagc cgcagcaccg acgacagctc cgccacggtc
     2341 aattcctcgc ggcgcagcag cgcgagcagt cgcacgcggg tagggtccga cagcacccgc
     2401 agcaacgcgg tggcggtaac cagatcgagc attccatctt tatatcagga tagagagatt
     2461 aattaaagca tggcgtccgc cgccgcctcg gtcgcatgcg cgacgggtta tgatggcagg
     2521 ctggattgtc gcgtctcgtg gcgcgggagc ggacaggacc aacggcgaga ggtagtgcga
     2581 tggatttcca gttctccgaa gaacagttgc agattcaatc gatcgcgcgt gatttcgcgc
     2641 agaagcggat cgccccggtg gcggcggatt tcgacaagtc cggcgaattc ccgctcgaga
     2701 acatccgcga gatgggcaag ctcggcttga tggggatcga ggtgccggcc gagtacggcg
     2761 gtgccgggat ggattcgatc agctacgtgc tggcgatgat cgaagtcgcc gcggccgact
     2821 gcgcgcactc gaccatcatg agcgtcaaca acacgctcta ttgcaacggc ctgctgaagt
     2881 tcggcagcga ggaacagaag cacaagtacg tgacgccgat cgcctcgggc ggggccatcg
     2941 gtgcgttcgc gctgaccgag ccgcagtcgg gttccgacgc gacggccatg cgctgccgcg
     3001 ccgccaggca ggacgacggc agcttcgtga tcaacggcaa gaagagctgg atcacgtccg
     3061 gtcccgtcgc cagctacatc ctgctgttcg cgatgaccga cccgggcaag caggcgcgcg
     3121 ggatcaccgc cttcatggtg gatacgtcgc gcaagggctt cagccgcggc aagaccgagc
     3181 ccaagctcgg catccgcgcg tcggcgacct gcgaaatcga attcgacgac tacatcgctc
     3241 agcccgacga ggtgatcggg gaggaaggcc acggcttcaa gatcgcgatg ggcgtgctgg
     3301 acgccggccg catcggcatc gcttcgcagg cggtgggtct cgcgagagcg gcctacgagg
     3361 cgacgttgga gtattcgcgg gaccggaagg ccttcggcca cccgatcggt tcgttccaga
     3421 tgacccaggc gaagatcgcc gacatgcgtt gcaagctgga cgcggcgcgg gtgcttacct
     3481 tgcgcgccgc atggctgaag caggaatcgg cgaagaccgg ggcgcgcttc tcgaccgagg
     3541 cagcggtcgc caagctgacc gcgtcggaag cggcgatgtg gatcacccac caggccgtgc
     3601 agatccacgg cggcatgggt tattcgcgcg agatgccgct cgaacgttac ttccgcgatg
     3661 ccaagatcac ggagatctac gagggcacgt cggagatcca gaggatggtg atcgcgcgca
     3721 acgagacagg cttgcgttga aaccgaaaac cgggtcgcaa aactggggtc agggtgcaat
     3781 ttcgaggaag cgacttggct aactcggagg ccgattcgaa attgcactct gaccccagtt
     3841 ttgcgctgac cccgggtttc gtcacttgcg cgtgcggtgc ggctccgcgt ccaccgagat
     3901 gaattcgggg tcttcgcggt cgagccgcag cgcggtgagc ttgccgcccc agacgcagcc
     3961 ggtgtcgatg gcgtagattc caaggccggc gaaccgcccc agcgccgacc agtgcccgca
     4021 cacgatccgc gtgtcgcgcg tccggacgcc gggcacctcg aaccatggat acagccccgt
     4081 gcgctgcgag cccggcggcc ccttcgcgtc gaaatcgatg cggccgtggg catcgcagta
     4141 gcgcatgcga gtgaagatgt tgattgcggc acgcaggcgg tcgtggccgt gcagccgcga
     4201 cgaccatcgc gccggccggt tgccgaacat ccgttgcagc aatgcgtggt ggcccgggcc
     4261 gcgcaaggcg cgctcgacct catgggcgat gtgctgcgcc tgcttcagcg tccaggacgg
     4321 cgcgaggccc gcgtgcacca gtgtgaaacc cagcgtggcg tcgtggtgga gcagtgactg
     4381 gccgcgcagc cagtcgatca ggacgggtcc gtcctcggct tcgagcaccc gccgcaggtc
     4441 ctggttggtg cgggctcggg cctccggttc gcgttccgcg attgccagca ggctgaggtc
     4501 gtggttgccg agcgcgacca cgcagtgttc gcgcaggccg tggaccagcc gcagcacttc
     4561 cagggagccg ttgccgcggt tgaccaggtc gccacagaac cacaggcggt ccgatgccgg
     4621 atcgaaccgc aggcggtcca gcaggcgctg gagttccggg tggcagcctt gcacatcgcc
     4681 gatcgcgtag atggccatgc gttcagcagc ccggcatcag tgcagggtgc ggggtaccga
     4741 cagtacgaat tcggggatcg gtacttcgaa gtcggtgccg tcgtcggcgt ggaagagata
     4801 ggcgccgcgc atggtcccga cctgggtttc gagcacggcg ccggaggtgt agcggtaatg
     4861 ttcgcccggc tgcaggcgcg gctgctcgcc caccacgcct tcgccgtgga cttcctcgac
     4921 cttgccgttg gcatcggtga tgacccagtg gcgggtctgc aggcgcaccg gcatcacgcc
     4981 gaggttgcgc agcgtgatct cgtaggcgaa cacgtagcgg tccgcctccg gtttcgattg
     5041 ctcggcgacg aagctcgcct cgacctccac ctcgaccgaa tacggttcgg gctcgcgcgc
     5101 atcgcgtggt tggtccgtgc tggtctggcg tgggctcatg aggggatggc gtcaaggggc
     5161 acccgcattg tcggcgagtt tcgtgccgcc ctgcaagttg gcggaggcga cctcccgcgc
     5221 aagcgcggcg aagccttgcg gcggcacgtt ctcggcgcgc acgcccgggt cgatacccgc
     5281 ggcgcggatc aggtccgggt cggcgagtcc acgcagcgcg ttggcgagcg tcttgcgccg
     5341 ttgcccgaac gcggcacgca ccacgttttc gatggcaagt gcgatcgggg catccgggcg
     5401 ctttgccgcc ggcagcggta ccagccgaac caccgccgaa tcgaccttgg gcggcgggcg
     5461 gaacgcgctt ggcgcaacgc gcagcagcgg ctcgacgcgg cacgcgagtt gcagcatcac
     5521 ggagagccgc ccgtacacct tgccgccggg ctgcgccgcc atcctttcca ccacttcctt
     5581 ctgcagcatg aagtgcatgt ccgcgatggc atccgcgaaa tcgaggcagt ggaacaggat
     5641 cgggctggac acgtagtagg gaaggttgcc ggcgaggcgc aagcgggcgg cacctgcgag
     5701 cgcgccgaga tcggttttca gcacgtccgc ctggatgacc tcgagttcgc cgacgcccgc
     5761 cgcgcgcgcg cgcagcggct cgatcaattc gcggtcgagc tcgatcgcgg tcaaacgccc
     5821 ggcggcgcgc agcaaaggga gggtcagcgc accctcgccg ggcccgatct cgacgatgcg
     5881 atcgccaggc ttgggcgcga ttgccgcgac gatgcgttcg atcaccgatc gatcgtgcag
     5941 gaagtgctgg ccgaagtgct tcttggggcg tggcatatcg ttcgaaggcg ggcgacaaga
     6001 aagcggatgg acgcggatga acgccgataa ggcaacaaag ctatcggacg cggataaaaa
     6061 cggatcgacg cggattggaa cgacggcttt ggatttggtc agatttcgat ccgcgtcgct
     6121 cgatgtcgac gcttctcctg ctttcgctgg atcagatggg agccgcgtca atggattctt
     6181 gttttcgcgc ggcaagttgc aaggccacgt ccgctgcggc gaacatgctc gatggatcgg
     6241 cgcggccggt gccggcgagg tcgagcgcgg tgccgtggtc gaccgaggtc cgcacgaacg
     6301 gcaggccgag cgtgatattg acggtgcgct cgaacgcctc ggatttcagc accggcagcg
     6361 cctggtcgtg gtacatcgcg agcacggcgt cgtagccggc gcgcgccgca ggcacgaaag
     6421 cggtatcggc gggcagcggg ccgagcaggc gcatgccttc gccacgcagc gtgtccatgg
     6481 ccggcacgat ggtgtcgatt tcctcgtggc ccaggtggcc gccttcgccg gcatgcggat
     6541 tcaggccgag cacggcgatg cgtggtccgt cgatcccgaa cttcccgatc agttccgcgt
     6601 ggacgatgcg cagcgtgcgc accagccgtt ccggggtgat tgccgccggc acctcgcgca
     6661 acggcaggtg cgtggtggcg agcgcgacac gcaattcggg cgaggcgagc atcattacca
     6721 cgtcccggcc ggcgcgttgc gcgaacagtt cggtgtggcc ggagaagcgc tcgccggcgt
     6781 cgttgatcac cgatttctgg accggtgccg tgaccaggct gtcgaattcg ccggccatgc
     6841 agccgtcggc ggctctcgcc agcgtcgcca gtacgtagaa ggcattgcgg ggatcgagcc
     6901 ggccgggcac ctcctcgcag cgcagcggaa cgtccagcac gcgcagcatg ccggccgccc
     6961 gcatcgtgcg cggacggccg tcgtaggctt cgatcttcag tgcgacgccg cagcggtgcg
     7021 cggcgcgttc gaacagcgat tggtccccga tcgcgatcag gtcggcggga atcggcgtag
     7081 ccgccagccg cgcgacgagt tcggggccga ctccggcggg gtcgccgccg gtgaccgcca
     7141 gcttccgcag tgccggcgga tgcgagccgg tcgcgaccgg ctcgccgttc attgcccctg
     7201 gtcggcggcc gcgggcgatg cgcccgccag ctcgggcagg cggatgctga tgtaggccga
     7261 cgactccagg tcgcgcagga actgctcgta cgcctgctgg cccttgcgcg cagcgatcgc
     7321 ctggcgcgcc tgctggcgtt ccaggtcccg ggtcttgtcc gcctggcgct cgccgagcag
     7381 ctcgatgatg tgccacgatc cgtcatggct ctggaacggc tggctcacct ggcccggctg
     7441 catgccggcg agcagcttgc ccacgtcgct gccccagtcg tcctgcatga accagcccat
     7501 gtcgccgccc gcgttggcgg tggtcggatc atccgactcc tgcttggcga gcttggcgaa
     7561 atcggcgtgg ccgtcgacga tttcctggta cagcttgttg atcttgtcct tggcctcctg
     7621 ctcgctcagc acggccgtcg gtttgatcat caggtggcgc gcgtggtact cggtgacgat
     7681 ctgcttggtg tcgggcgtgc gccggccctc gagcctgagg atcgtgaagc cggacgcatc
     7741 acgcagcggc tgggtgtggt cacccggttg aagggattcg acgacctgct gcaccgcggg
     7801 cgacagctcg tcgacgcgcc gccagcccag atcgccgccc tccagggctt ccggcgcgct
     7861 ggagttgctg accgcgagcg cggcgaaacc cttgccgtcc ttcagctcac cctcgatctg
     7921 ctcggccttg gccttggccg cggcgatctg gttggcgtca gcgccttccg gcaagccgac
     7981 caggatgcgc gcgaggtgga cttcgccctt cttgaacgcg gggctcgcga tcaggttctt
     8041 gatctcggca tcggaaacct gcccgttgga ttccatgacc tgctgctgca cctggcgcac
     8101 caggaactgg tcgcggagtt ggtcccggaa cgaggcgtaa tcgatgccct gttgctgcat
     8161 ggcggcctgc atctgcggca ccgtcatgtt gttctgcttg gcaatgttgg cgactgccgc
     8221 atcgacctgt gcatcgggaa tgcgcaggcc gttttcctgg gccttctgca ccagcagttc
     8281 gttgaggatc aatcgctgca gcacctgctt ctccagcacc tcccgcggcg gctggcgccc
     8341 gcccgaagcg gagatctgct gggccacggt ggccatcgtc tggtcgagct ggctttgcag
     8401 gatcacgccg tcgttgacga cggccacgat ccggtcgagc ggctgcacgc cgttgggggc
     8461 ggccgcttgc ggggccagca gggaagagtc ctgggtgccg gtgggcggcg gcgcgttcgg
     8521 gctcagcagt tgcgcgaacg cgggcagcgc gaatgcgcag gccgcgaacg acagcgccag
     8581 gcgggcgaaa aagcgggaac gggtcatttt gctcatgggc gggaaggtcg tatgggcgtg
     8641 ccggaccaag cggtccgatg gaagcgaagg gttggacagc gccagtcgtg aaaaagttga
     8701 gttgtcgagc ggttgttgtg aaaagtcagg gccgggacgg acgcttacgc gatgccgggg
     8761 gcatcgcgtg cccggccaag cgaccggcca cggatggccc ggccggcgcc cggcgccatg
     8821 gacggctgct tgctaaaggc atttgcggtc accgccgatc atggacgatc gcacgattat
     8881 tgatagccaa ggatagcagt ttgcaggaag ctgccgaggt gctgcccata actgccgagg
     8941 cccttgaact ggatctcgaa catgatcgcg tcgtcggagt ggccctggta gtcgtagacg
     9001 taatgccgcg acaggatccg gatggcggtg cagcaactgt cccattcgac gcccgcgaag
     9061 gcgtcgaggg ccatctggtc ccggaacgag tagttgtatc ccgccaccag cttccaggcg
     9121 gcgccgaccg ggacttcggc ggagacgtcc gcctggtcga gcaggttgcg gcggtagcgg
     9181 taactgaggt tcacgatgcc gtcgccccac agccggcgct gcaggcccac ggtcgagacg
     9241 tccgtctggt tggtgttcgg gttccattgc tgcgacgccg tcagccgcca gttgtcgctg
     9301 aggctcaacg tcaggttggc gacatagttg gatccggcgt agtcgacctc cttgccgccg
     9361 cgtcgcaagg tgacgcgctg cggttcgaag tagcggatct ggccgatgcc cgctgagagt
     9421 ttctccacgc cgttctcgtc gaggaagcgg gtggtcagcg ccagcgacag gttgttggcg
     9481 ttcatctggc ggtcggcgcc gctgaaggtg ttggtggtga agagctgcca gaagtcgaag
     9541 gtcatctgcc gcgtgtcgaa cagcggtatg tcgtcctggt cgcggtacgg cacccgcagg
     9601 tagtaggcac ggggttccag cgtctgggtg tactcctgcc cgaacaggtg gacgtcgcgt
     9661 tcgaacacca ggccggcgtc gaggtcgaaa atcggcgtgg tgcgcgacgg atgcgtcgag
     9721 tagcccttga ccaggggccg cggcaggttg tacgtcgtgt agcgcaagcc gagttcgggc
     9781 cgcaggaacc aggccgcgcc ctgcagcggc caagcgaggt acggatagaa atcgaagcgg
     9841 tcgcctccga tctcgtccgg cttcacgaaa tgcaccgctt cggtttccgt gccccactcc
     9901 ggcccgccgg tgccgccgat gggtccgcct gcgttgaagt acacgcgcgg caggcgccgg
     9961 tacgcggcgg agcggtcgga aaggctgggg tcggcgatct ggtagaaatc ggctccggcg
    10021 cccgcgttcc accaatcgcc cgaaccgttc acgtacgacg atgagtgcaa ctggcgcagg
    10081 ttcgaggtcc tgaagttctc gccgtagtcc tggtaccagg ccttgtcgga aatccagttg
    10141 atccgcgtac tccagcgcag gtcccagggc aggcgcgtct ggttggcgag gatgaggtgg
    10201 gtccggtcgg tgtcggtgac cttgtcgtgc ggtatgtagt cgaggtccaa ctcgcccttg
    10261 ctggtcgagg tcagccagcg gaactggctg tcgagcatcg cgccgcgctc ggcgtaccag
    10321 gtcggcgtga aggtggcgtc gtagttgggg gcgatgttcc agtagaaggg aatcgagaag
    10381 aaatagccgg cgcggctgcg cttgccgaac gaggggaaga ggaagccggt ctggcggcga
    10441 tcgtcgagcg gaaagcgcat gtacggcagc cacaggaacg gcacgccctt gagccgcatc
    10501 gtgacgtcat gggcgcgccc gagcccctcg tcgcggtcca tggtgatggt cttcgcctcg
    10561 atctcccaca cccggtcatg cagctcgcag gtcgagtagg tgacgtcgtg cagcttgtca
    10621 cgatcctggt ccagcatgat cgcccggctg gcgacgccgt tgccgtggga atccagcaac
    10681 tggtactgca cgttgtcggc aacgccgtac tcgggggtgg tggtcccctt catgctgtcc
    10741 gccgagaaca acatcccacg ttcctggtaa cgcacgtcgc cttcggcatc gtatgccgtg
    10801 gtctcgcggt tgtaggtgag gttgtcggcg cgcagcaact ggtcgaggcg ctgcagcgag
    10861 gtgtgccccg acaggtggaa caggttgctg tccgggctgc tgaacgtgtc gccgaacacc
    10921 tgcgcagggc tggcttcacg atcgccggtg gttggcaagc cgggctggta gaaatccagc
    10981 aggtcgttct tcttgcacag cgcgaaatcg ttcgtggcca ccggacacac gaacgcgccg
    11041 agcgggcaga ccctgggacc ggtctgcgcc agcgccatcc cgggtgccgc cgccagcgtg
    11101 atcgcgaacg ccagtgcgcg cggtgtgggc ggcttgattc ggcgcagcac gggcattcct
    11161 ggcaatcgaa tccgcgtaag ctagcagaat gtcgcgcgcc gggtcagcag gctgggtgcc
    11221 gtattcaggc tgcccgggcc gtggaaagcg gtgcgcagcg cgcgtccagc caggcacggt
    11281 cggcgggatc atcgagcagg ggcgcgagcc gctcgcgcac catcgcgtgg tagccgtcga
    11341 gccacgcggt ttcggctgga tccagcagcg ccatgtccag cagccgggtg tcgatcgggc
    11401 acaaggtcag ggtttcgaac gcgaggaact cgccgaactc gccgtcgccg gcggacaccg
    11461 tcgccgcgag attctcgatc cgcacgccat ggcggccggg cttgtagatg cccggctcga
    11521 tcgaggtgat catgccgacg tccatcggct cgagctgcga ggccgagcgg ggtgggcgga
    11581 tcgactgcgg gccttcgtgc acgttgaggc agtagccgac gccgtggccg gtgccgtggc
    11641 cgtagtcgat ccccgcggcc cagatcggcg cgcgggccag ggcatcgagc tgctgcccgg
    11701 aggcgccgcg cgggaatttc gcgcgcgaca gcgcgattac ccccttcaac acgagcgtca
    11761 cgtcgcggcg ttgctcgggc gtcgtctcgc ccagcgccca catgcgggtg atgtcggtgg
    11821 tgccgccgag gtattgcgca ccggaatcga tcagcagcat gccgcgggcc cggagctcgg
    11881 catgcgattc cggcgtcgcc gaatagtggg gcagcgcggc gttggcctcg tagccggcga
    11941 tcgtggaaaa gctttcgctg acgaagccgg gttgctgcga gcgcaacccg cgtaattttt
    12001 cgtcgacgtc gagttcggtg agccgccggc cgtccttgag cgattgctcc agccagcgcg
    12061 cgccgcgaac cagcgccacg ccgtcgcggc gcatcgcttc gcgcacgtgc tccatttcct
    12121 tgtccgattt gcgcgccttg gccgcggtga tcggaccggg cgcttcgatc accacgacga
    12181 tttcgggaat cgcgtgcgcg agggcggcgg agacctgccc gggagccagg agcagccggg
    12241 tgtccttcga caattcgcgc aacgcgtcgc tcgccgcttc gtagggcgcg acgcggacgc
    12301 cttcgtccgc caggcgcgat cgcagcttcg catccagttt ggccgcatcg acaaacacgg
    12361 tcgcttcgcc cgcgccaatc aacaggtgcg cgaggaacac cgggttgtaa tcgacgtcgc
    12421 tgccgcgcag gttcaggacc caggcgattt catccagggc ggagacgacg tggtggctcg
    12481 cgcccgacgc ccgcatggct tcccgcacgt cgccgagttt ctccgcgcgc gacctgatcg
    12541 cgtagtcgat cggatgctcg tacaccggcg cgtgcggcag gggcggccgg ccgctccaga
    12601 ttgcgccggg caggtcgtct tcgaccagct ccccgccgtg cgccgaaaat tgctcgcgca
    12661 gcgaatgctc tgccgcgagc gacagcatgt ccgcggcgca cgccaccttg tcgccctcgt
    12721 ccaggcgccc gcacagccac gcgacgtgct cggcagtgtg cggcacgttc agcttcatga
    12781 gctcgatgcc gctgccagcg agctggcgct cggcctgcga gaagtagcgc gagtcggtcc
    12841 acaggccggc gaagtcgcgg ctgaccacca gtgtgccggc cgagccggtg aagcccgaca
    12901 gccattcgcg cgcctgccag cgggcgggca ggtattcgga aaggtgcggg tcggcggtgg
    12961 gcacgatgca cgcggcgatg ccgcgctcgt gcatcacgtg gcgcagcgcg gcaatgcgtt
    13021 ggggaatgtt ggcgtcggtc atggtgtcgg ccgaggtgca ggttccttca tgttctcgcg
    13081 tcaggaggat cgctgcaaac cgggttgcgc gttggttgtg cggggcgctt ccggtagaat
    13141 gacgctttcc acacacggtc cgatcatgac ctccctgatt ttcgtcaccg gcggcgtggt
    13201 gtcttcgctt ggcaagggca tcgcgtcggc gtcgctggcg tccatcctcg aggcccgcgg
    13261 cctcaatgtg acgttgatga agctggaccc gtacatcaac gtcgatcccg gcaccatgag
    13321 cccgttccag cacggcgagg tgtacgtcac cgacgacggc gccgaaaccg acctcgacct
    13381 cgggcactac gagcggttcg tccgcaccag gctgtcgggc aagaattcga tcaccaccgg
    13441 caagatctac gaagcggtga tccgcaagga acgccgcggc gattatctcg gcgcgacggt
    13501 gcaggtgatc ccgcacatca cgaaccagat caagcacgcg atcgacgagg ccacccgcgg
    13561 cttcgatgta gcgctggtcg agatcggcgg caccgtgggc gacatcgagt cgcagccgtt
    13621 cctggaggcc gcgcgccagt tgcggatcga gcgtggcgcc gacaacgtgt tgttcatgca
    13681 cctgacgctg gtgcccttca tcaaggccgc cggcgagatc aagaccaagc cgacccagca
    13741 ctctgtgaag gagttgcgcg cgctcggtgt ccagccggac atcctggtct gccgctgcga
    13801 ggagccgctg ccggaatcgg agcgccgcaa gatcgccctg ttcaccaacg tgcccgaaag
    13861 cgcggtgatc agcgcggctg acgcggacac gatctacaag ctcccgttgt ggctgcacaa
    13921 ggaagggctc gacgacatcg tggtcgaacg cctgcacctc gacgccaagc ccaccgacct
    13981 ggctgagtgg cggcgcacgg tggatgccgt cgaaaacccc agggacgaag tgcgcgtcta
    14041 catcgtcggc aagtacgtcg agcacaagga cgcctacaaa tcgctcggcg aagcgctgcg
    14101 ccacggcggc atccacaacc agacccgggt gaatctcgag tgggtggatt ccgagatcat
    14161 cgaaacgcgc ggcgcggacg ccgtgttgca cgacgccgat gcgatcctgg tgccgggcgg
    14221 cttcggcaag cgtggattcg agggcaagat ccagaccgtt cgttatgcgc gcgagcgcgg
    14281 cattccgtat ttcggcatct gttacggcat gcacgcggcg gttgtcgagt tcgcccgcga
    14341 tgttgccggt ctgtccggcg cgggatccac cgagaacgac cgcgactgcc tcgagcccgt
    14401 gattgcgctg atcacggaat ggacgcaggc cgcgggcgac gtacagcacc gcgacgagac
    14461 atccgatctc ggcggcacga tgcggctcgg cgcgcaggaa tgccgcctga aaccaggcag
    14521 cctggcgcac gagctgtacg gcgcggacac gatccgcgaa cgccaccgcc atcgctacga
    14581 attcaacaac cgctatcgcg aacggctgga gaaggcaggg ctggtgatcg cgggcgtatc
    14641 cgtggacgac ctggtggaga tcgttgaatt gccgcagcag aagcacccct ggttcctggc
    14701 ctgccagttc catccggaat tcacttcgac gccacgcgac ggccatccgc tgttcaacgg
    14761 gttcgtgcgc gccgcgcgcg agtacaaggc catgcgcgat gcgccccggc tcgccaagga
    14821 agccgccgga tgaatttgtg cggcttcgac gtcggcctcg accagccatt cttcctgatc
    14881 gccggcccct gcgtcgtcga atccgagcag ttgcagatcg acaccgcggg ggccctgaag
    14941 gacatcaccg gcaggctcgg cgtcaatttc atcttcaagt cgagcttcga caaggccaac
    15001 cgcacctcgg gcaagagctt ccgcgggctc gggatggagg aaggcctgcg gatcctcggc
    15061 gaagtgaaga aacagctcgg cgtgccggtg ctgaccgacg tgcacgagta cacgccgttc
    15121 gatgaagtcg ccgcggtggt ggacgtgctg cagacgccgg cattcctgtg tcgtcagacc
    15181 gatttcatcc agaaggtcgc ggctgccggc aggccagtca acatcaagaa gggccagttc
    15241 ctgtcgccgt gggacatgaa gaacgtggtc gagaaggccc gtgcggtcgg caacgaccag
    15301 atcctggtgt gcgaacgcgg cgcgagcttc ggctacaaca atctcgtgtc ggacatgcgt
    15361 tcgctggcgg tgatgcgcga aacccactgc cccgtggtgt tcgacgccac gcattcggtg
    15421 cagttgccgg gcgggcaggg cacgaagtcc ggaggccagc gcgaattcgt gccggtgctg
    15481 gcgcgggccg ccgttgcggt gggcgtcgcg ggcgtgttca tggaaaccca tcccgatccg
    15541 gacgaggcgc tgtcggatgg cccgaacgcc tggccgctcg acaaaatgga agcgctgctc
    15601 acgacgctca aggaactcga cgcggtcacg aagcacaacg ggttccttga aatgcgaagc
    15661 tgatctggac gcgggtcaac gcggattgat tttgccttat ccgccttgat ccgcggcaat
    15721 ccgcggcaat ccgcgtcaag ggcttcttct ggagtgattc atgacgacag caatcaagac
    15781 gatccacgcc cgcgaagtgc tggacagccg gggcaatccc acgctcgaag ccgaagtgac
    15841 gctgggcgac ggcgcgttcg gtcgcgcgat cgtgcccagc ggcgcatcca ccggcacgcg
    15901 tgaagccgtg gaactccgcg atggcgacaa ggcgcgctat ctcggcaagg gcgtgaccag
    15961 ggcggtcggc aacgtcaatg gcgaggtcgc caaggcgctg gccgggttcg acgcggccga
    16021 ccagaagggc ctcgatgaaa agctgatcgc gctggacggc acgcccaaca agtcgcgcct
    16081 cggcgccaat gccacgctgg gcgtgtcgct ggcagcggcg cacgcggtcg cggcctcgcg
    16141 caggcagccg ttgtggaaat acctggccgg cgaccgcgcg ccgatgctgc ctgttcccat
    16201 gatgaacgtc atcaacggtg gcgtgcacgc ggacaatccg ctcgacatgc aggaattcat
    16261 ggtgatgccg gcgggtttcg gaaaattctc cgacgcgctg cgcgcgggcg tcgaggtttt
    16321 ccatgcgttg aagacgcagc tcaagaagcg cggcatgaac accgccgtgg gtgacgaggg
    16381 cggcttcgcg ccggcgttgc gttcccacga ggaagcgatc gaggtgatcc tggaggcggt
    16441 gaatgcggcc gggtacacgg cgggcgaaca ggtctggatc gcgctcgacc ccgcctcgtc
    16501 ggagttcttc gaccacgggg tgtacgacct caagggcgaa aaccgcaagc tcgatgccgc
    16561 cggcatggtc gggctgtatg ccgactggtg cggcaagtac ccgatcatca gcatcgagga
    16621 tggcctggcc gaaggcgacc aggccggctg gaagctcatc accgaaacgc tgggccagaa
    16681 gatccagctg gtcggcgacg acctgttcgt caccaacccg tcgatcttcc gggaaggcat
    16741 cgccaaccac atcgccaacg cgatcctggt caaggtgaac cagatcggca ccttgagcga
    16801 gacgctggag gccgtcgcga tggccgacca ggcggcctat gccgcggtga tttcgcaccg
    16861 ttcgggcgaa accgaggaca ccacgatcgc ggacctcgcg gtggcgacca cctcgactca
    16921 gatcaagacg ggttccctgt gccgctcgga ccgcatcgcc aagtacaacc agttgctgcg
    16981 gatcgaggaa caactcgggt ccggcgcgaa gtatgctggc cgcgatgcgt ttcccaatct
    17041 caaggctctt cccggctgag gccgtcgccc cttgcttcgt tggatagcgt tggtcctgat
    17101 cgccctcctg atcggcttgc aggtcaagct gtgggtgggt gacggtggca tgcgcgacct
    17161 gcgtgcgatc cgcgcgcgcg tttcgtcgca gcaggccgag aacgccaagc tcaagcagcg
    17221 caatgaagcg ctcggcgccg acgtcgaaga cctcaagcac gggcaggatg ccgtcgaggc
    17281 cagggcgcgc tcgcaattgg gcctcatcaa gccgggcgaa gtgttctacc aggtcgtggt
    17341 ggcgccctcc ggcagcagcg cggcggcgtc gcctgcggcc gcgagtaccg ccgcagcttc
    17401 cgcccactga tgaaccgttc cgcggtgacg tatcggctaa actgatcgct gcgcgcgtcg
    17461 cgcaaggttt cggtttgggg ttcgactgca tgcggatgtt gctgagcaat gacgatggcg
    17521 tggatgcgcc cgggctgggc gtgctcgccg agcggctggc ggaggtgggc gaggtgacgg
    17581 tggtggcgcc ggatcgtgac cgctcgggcg ccagcaattc gctcacgctc gaccagccgg
    17641 tgcgggtcgc gcggctggac gaacgccgtt accgcgtggt cggcacgccc accgactgcg
    17701 tgcacctcgc gctcagcggc ctgctcgacg gtgaacccga catcgtcgtc tcggggatca
    17761 acaattccgc gaacctcggc gacgacgtga tctattcggg cacggtttcc gccgcgatgg
    17821 aagggcgctt cctgggactg cccgcgatag cggtgtcgct cgccggtatc ggcgaacgcc
    17881 atgacggcaa cggccaccac ttcgactccg cggcacgcgc ggtattgctg ttgatgcgcc
    17941 ggctgctggt ggacccattg ccggccgaca ccatcttgaa cgtcaacgtg ccggattgcc
    18001 cgtggtcggc gatacgcggc ttcgaggtga cccgcctggg ccggcggctg cgctccgaac
    18061 cgtgcgtccc gctcaaggat ccgcgcgggc gcacgctgtg gtggatcggc gcggccggcg
    18121 aagccgacga cgccggtccc ggaaccgatt tcgatgccgt gcgtcgcgga ttcgtttcgg
    18181 tgacccccat ccacgtcgat ctcacgcgct tccaggcgct cgaaaaggtg tcgagctgga
    18241 tcggcggttt gaccgcgcaa atggaggggg agcaggcggc atgaacacgt tgttcggagc
    18301 ggtggctgcc tccgcgggcg gacgcggcat gacctcgcag cgcgcgcgcg accggctggt
    18361 cgcgacgctg cgcggcgagg gcatttcgga cccgcgcgtg ctcgacgtga tccgcaccac
    18421 gccgcggcac ctgttcatcg acgaagcgct tgcctcgcgc gcctacgaaa acaccgcgct
    18481 gcccatcggc ctggggcaaa cgatttcgca gccgtgggtg gtcgcgcgca tgaccgaatt
    18541 gctgatcgag cgagcgatcc ccgaccgcgt gctggaggtc ggcaccggct cgggttacca
    18601 ggccgccgtg ctctcgcaac tcgtcggcac cgtctatacc gtcgaacgga tcgaggagct
    18661 gctgcgcaag acgcgccggt tgttccgcac gatgaagctc gagaacatcc gcacccgcca
    18721 tgacgacggc aacatcgggt ggacggccga agcaccgttc gacgcgatag tgttgaccgc
    18781 agccggcgaa accgtgccgc gcgcgctgtg cgagcaattg gccgagggcg gggtgctggt
    18841 ggcgccgttg ggaccggccg gcagccagcg cctgacgcgc atcagtcgcg aaggcgagtc
    18901 atggccgcgc gaggaattcg aagcggtaag cttcgtgccg ctgttgggcg gcctcggttg
    18961 atggttcctc gccacgcata caagcccttg ggatgcagcg accaaggacg atcgcaccag
    19021 tgaaagtctt ccggccgctt tacgagtggg cgctgaaatg ggccgccaag ccccgcgcgg
    19081 acgcgtggct gtgcgtgctg agcttcttcg aggccttcat cttcccggtc gcgccggaaa
    19141 tcatgctggc gccgatgacg ctggcgcgcc cgaagcgctg gtggtatttc gcgacgatca
    19201 gcctggccgg ctctctggtc ggttcgatga tcggttacga actcggtcat tacgccttcc
    19261 attacctgca accgttcctc gccgacatcg gctggtcgca gcacctggat gcgatggtgg
    19321 agaagttcgg cacggcggcg aagcggcatc catgggaagc tttctggatg ctggtcgcgg
    19381 ccggcttcct gccgatcccg atgaaggtcg cgagctgggc ctgcggcatc gtcggcatgc
    19441 cgatcctgcc gttcctcgcc ggcatcctga tcggccgcgg caagcgcgtg ttcctggtcg
    19501 cgggcgtgat ccgcctcggt ggcgagcgtg ccgagaaggc actgcacaag cacatcgaaa
    19561 ccgcaggctg ggtgatcgtc gcgatcgtcg tggtgctggg gttgtatttc tacctgcggt
    19621 agttgccgac aaatccgttc gggagcagct tcttggcgga aagtcaggcc aaggatggcc
    19681 gttctggagt ttccaaatgt cgagcgctcc gtccacgcca tggctggtgg agcagcgatc
    19741 caggacgatc gcacgaatac gttatccttg cgcgcatcat gcgaacgatg aaaccaatga
    19801 aggtactgtt tgccgccgtg gcgagtgcgg ccttgctggc gggctgcgcc tcgacacctg
    19861 cgccggtggt caaccattcc ttccggccgg gggcgccggc atcgccgggc gctcccgcgt
    19921 cgcagccggc ggcgtcgaat gcagccggaa cacagacctt cgggctgggc cacgccaacg
    19981 gcacggtcag cgcggccggt tacaccgtcg cgcccggcga cacgctgtac agcgtcgcgt
    20041 tcgcgcatgg catggatgtg cgtacgcttg cggcgcagaa cgagatcccg ccgccgtacg
    20101 tgatccgtcc gggccaggtg ctgaaactgg aagccccgca agccgcgggc gccacggccg
    20161 ccgcgggcac gaacgcgatt gcacccccgc ccgcgccggc aacacagtcc acgtcacccg
    20221 tgttcggccc cgttaccacg caatccatgg cggcgaatgg cgtcgccccc gccgcatcgc
    20281 ctccggccgc gagcacggct ccggtgttcg ggccagtcac cacgcaacag atgacccccg
    20341 gcggcgtcgc gtcggcgtca tcgtcatcgg ccgtggtcgt ggcggagtcg ccgcctgcgg
    20401 cagcgaacgt gccgcccgtt tccgcggcgc ccgtccctgc aatgacaccc acgccgcccc
    20461 ccggcacgac ccgcaccgca gccgggatca cctggcggtg gccggcgagc ggcaaggtcg
    20521 tcggcggttt caaggcaggc gtgggtggcg atggcgcggg ggtcgacatc gccggcaacg
    20581 tcggcgatcc cgtgcgcgcc gccgcagacg gaacggtcgt gtacagtggc aacggcctga
    20641 tcggctacgg cgagctcgtg atcatcaagc acaacgacac gtatctttcc gcctacggcc
    20701 acaacagccg gcgcctggtc aaggaaggcg agcacgtcga tgccgggcag gagatcgcgc
    20761 tgatgggcgc gagcggtgcg ccgcgcgtgg aactgcactt cgagatccgc aaggacggca
    20821 agcccgtcga cccgttgggt tatttgccgg atcggtaatc tatcgaaccg cgctggtgtg
    20881 gtccgaagcg gctgttgtgg aaattcaggc catggaaggc cgttctggtg atgcccggga
    20941 atgaagcggt gccggcgtcg ctatggcatg tggcgcagcg atcagggacg atcgcacaaa
    21001 acaaacgccg cgaccacgtt gcgaccttcg ccggcgagga tgtcccaggt gcgggccgcg
    21061 gcggcgttgt ccatcgcttc gaggcctatg cctcgttgca ggaaggcggc cagtacctcc
    21121 tgcgggggaa actgctggcg cgcgccggtg ccgagcagca ccacgtccgg tttcagcgcg
    21181 acgatcggtt cgatgtgccc tggcgtgatc tccgcgacgt gggcaatccc ccagtcgtcg
    21241 atgacttgcg tccgggtcag gatcaggctt cgcgtgaacg cacgatcgcc cacgatgacc
    21301 gagtgcgcat cggccttgcg cacgaacagg aagtcatcgg gacgttccag gttcagttcc
    21361 acggctgctt atttcggcag cgcgatcttc ggctgctcgt cgttgcgccg gtacagcacc
    21421 gcgacatgcc ctatggtctg cacggtctcg gcatggctcg attcggcgag cttcatgact
    21481 tccgccgcgc gcgcggcgcg gtccccgccg ctcaggcgga ccttgatcaa ttcgtggtcg
    21541 gcgagcgcct gctccagttc cgtgagcaag ctgggcgtga cgcccttgcc gcccagcagc
    21601 accaccggtt tcagcgggtg ggccaagccg cgcaggtagc gggtctgtga cgcagtgagc
    21661 atgtgttcag cgatgcgggg cggaagtgcg gcctgacagg gtatcatggg gtcgtgtcgg
    21721 aaccggcgtg cgcaacccat ggccaggtcc aaaagcagcg cgcgctggct gaaggaacat
    21781 ttcggcgacg aatacgtcaa gcgggcacag gcggagggcc tgcgctcgcg ggcagctttc
    21841 aagctggacg agttgctgga gcgcgaccac ctgctgaagc ccggcatggt ggtggtggac
    21901 ctcggggccg cgccgggcgg ctggtcgcaa ctggtgcgcc agcggctggg cgacaacggc
    21961 cgggtatttg cattggacat cctgccgatg cagggcatcg gcggtgtcga gttcctggag
    22021 ggcgatttcc gggaacagcg ggttttcgat gccttaatgg agaggctggc gggaagccag
    22081 gtcgaccttg tcctgtcgga catggcgccc aatatcagtg gtatccaagc ggcggatcag
    22141 gcccgcgcga tggagctggc ggaactcgca tcggatttcg ccggcaagtg tttgaaaccc
    22201 ggagggtcgt ttctcgtgaa gttgttccag ggccgcggct tcgacgaata catacgcggc
    22261 ttgcgcgcag gcttcgggcg ggtgacactt cgcaagccca aggcctcgcg cgcgcgttcg
    22321 cgcgaagtgt acgccctggc cacaggcagg aagagttgaa acatgaatca ccttgaaacc
    22381 gatccccagg ctgctgttcg cgagaccgtg catgcgcacg ccggctcccg ggcaggaggc
    22441 gcgcgaggat gaagcccccc atgaacgacc tgaccaagaa catcctgttg tggctggtgg
    22501 tcgcggtgat cgtggtgctg gtttaccaga gcctcagccc gcgcatgtcc ggcggcgagc
    22561 aggagatgtc gtattccgag ttcgtgcagc aggtgaagaa cgacaacgtc tcttcgatca
    22621 cgatcagccc gaacatgccg ccggtgatca ccggcaagct caaggacgga aacgacctgc
    22681 gcacggtgct gccggcggtg ggcgacaaca gcctgttgcc gttgctccag tcgcaccgtg
    22741 tcaagacgac gcagaagccg ggcgacaacg ggttctccct gatgcggctg atcatcgagt
    22801 ggctgccgtt catcgtggtg ttcggactgc tgttctacat ctttcgccag atgcaatcgg
    22861 gtgcgggcgg gcgcggcgcc atgagcttcg gccgctcgcg cgcaaagctg cagggcgagg
    22921 accagataaa ggtcacattc aacgacgtgg ccggctgcga ggaagccaag gaggaagttt
    22981 ccgaactggt cgacttcctg cgtgacccat ccaagttcac caagctcggt ggcaagattc
    23041 cgcgtggcgt gttgatggtc ggctcgccgg gcaccggcaa gacgctgctg gcaaaagcca
    23101 tcgcgggcga agccaaggtg ccgttcttct cgatctccgg ttccgacttc gtcgagatgt
    23161 tcgtgggcgt cggcgccgcc cgcgtgcgcg acatgttcga gcaggccaag aaacacgcgc
    23221 cgtgcatcat cttcatcgac gagatcgacg cggtgggccg tcatcgtggc gccggcctcg
    23281 gcggcgggca tgacgagcgc gagcagaccc tgaaccagtt gctggtcgag atggacggct
    23341 tcgagggcag cgaaggcgtg atcgtgatcg ccgccaccaa ccggccggac gttctcgatc
    23401 cggcactgct ccgcccgggc cgtttcgacc gccaggtcgt ggtgccgctg ccggacctca
    23461 agggccgcga gcagatcctg aaggtgcaca tgcgcaaggt gcccgcgggc gccgacgtcg
    23521 atccgctgac gatcgcgcgc ggcaccccgg ggttttccgg cgccgacctc gccaacctcg
    23581 tgaacgaggc ggcgctgttc gccgcgcgcg agaatgcccg cgacgtgcgc atggatttct
    23641 tcgagcgcgc caaggacaag atcatgatgg gctccgagcg gcgctccatg atcatgagcg
    23701 aggacgagaa gaagctcacc gcctaccacg aatcggggca tgcgatcgtc ggcctgtcgg
    23761 tgcccgacca cgaccccgtg cacaaggtga cgatcatccc gcgcggccgg gcgctgggcg
    23821 tcacgatgtt cctgccggaa caggatcgtt acagccacag caagctgtcg ctggaaagca
    23881 agctcgcgag cctgttcggc gggcgcgttg ccgaggaact gatcttcggc agcgagaagg
    23941 tcacgaccgg cgcgtccaac gacatccagc gcgccaccgc gctggcgcgc gacatggtga
    24001 cgaagtacgg cttgtcgacc gaactcggtc cgatgaccta cacggaggac gaggacgagg
    24061 tgttcctggg ccgctcggtc acccagcaca agaacgtgtc ggacgaaacg tcgcgcaaga
    24121 tcgacgttgc cgtgcgcgca gtcatcgacg ccgcatacag tcgcgcgcgc cagatcctca
    24181 ccggggacat caccaagctc catgcgatgg ccgccgcgct cctgcagtac gaaaccatcg
    24241 acagggaaca gatcgcggca atcatggaag gccgtgagcc cggcccgccc aaggactgga
    24301 ccccgcgcgg cgacaacacc ggaatgggcg gcggcaagcc tgcgacggca ccgggcgttg
    24361 cacctgcgcc ttccagccat cccgccgggc agcattgacc ggtctcgcaa tccgctgaaa
    24421 ggtcgccccc cggggcgacc ttattttccc gggctggacg gttgccgcgt cgctggacgg
    24481 cgtacccgat tccaccaccg ccgcgcggcg accggtggtg ttgcaggcgt gtagcatagg
    24541 cgcacgaacc ggtgcgcgcc gacgacgccg accgcccatg ctcgcgaaac tgaagctcac
    24601 cttctccgaa ttccggcgcc gccgcgtgtt ccgcgtcgcg gctgcctacg tggtggcggg
    24661 ctggctgctg atccagctcg gcagcgcgac cttcgaaccg atgggcttgc cggactggtc
    24721 gatgcgcttc ctgctggtgc tgctggtcct cggcttcgtg ctggcgtgcc tgttggcctg
    24781 ggtatacgac tacggtgcgc acggcctcga aaggacattg cccgccaggg ttccactacc
    24841 cgcttccgac ggccgacatg ccgccgcgcc ggatgcttcg gtcgcggtgc tgccgttcgc
    24901 ggacctcagc gaggcgcacg accagggcta tttctgcgat gggctggccg aggagatcct
    24961 caacgcgctc gcgtcgatca aggacctgcg ggtggcttcg cgcacgtcct cgttccgctt
    25021 ccggaaccgc gatgcggatg cgcgtgagat cggccacgcc ctgaacgtgg ctgcgatcat
    25081 ggagggcagc gtgcgcaagg ccggcgatcg cgtacgcgtc accgcgcagt tgatcgacgc
    25141 cgcgaacggc taccacctgt ggtcggagaa cttcgatcgc aagctcgagg acatcttcgc
    25201 gatccaggag gagatcgggc gcaacgtggc ccgcgccctg cgcgtgtcgt tgaacggcgc
    25261 cgcgaaactc gatcctggtt gtacctgcaa tgcgccgcgc gacatgcgtg cctacgaatt
    25321 ctacctgcgc ggccgccaga tgcagtcgtt gaagagcagc gacaactgga ccaaggcgtc
    25381 ggagatgttc cgccgcgcca tcgaactgga tcccgactac gcgcatgccc atgcaggcct
    25441 cgccgattcg ctcgcgcaat tgctgatctg gcggatcctt cagcccgaac gggtactcga
    25501 tgaagccgtc gcgtcggcca agcgcgccct ggagctggcg ccgaacctcg ccgaagccca
    25561 cgtggcccgc gccaacacct tgtcgctggc cggtgacaac caaggcgcgg tcgccgcctt
    25621 tcgtcgctcc atcgaactcg atccggagct gtacgaagcc cattactatt tcggccggca
    25681 ctgctatgcg cggggcgact acgtgctggc gatcgaggag ttcgaggccg cgcaccgtgt
    25741 gcgtcccgac gaattccagg cgctgtcgct ggcttccaat gccgcggatt cactcggcga
    25801 cacgggacgc ggcgacgaac tcatccgcct cgcgctgccg cccgcgctcg ccgagatcgc
    25861 ccgcgatccg gagaacggcc gtgcgcttta tctcgccgcc ggcatgcaag tgcgactggg
    25921 cgacatcgaa ggcggtcgcc gcaacgtcga ggcggcgttg cgcctgcagc cggacgattt
    25981 cggcacgcta tacaatgccg cctgtaccta cacgcacatg ggcgacaccg atcgcgcctt
    26041 cgatcttttg gagcgcgcga tttcggtggg ccatggtttc cgcgactgga tcgaacacga
    26101 ttcggacctg gacagcctgc gcggcctgcc gcgctatggt gagatcttgg cgcggcttcg
    26161 caatcccgga gattgaagca gcgacatgtt cgacaccact cccttgctcg actgcaacgg
    26221 tcgccggctg gaactcgatc atccgcgcgt ctgcggcatc ctgaacctta ctgatgattc
    26281 gttttcgggc gacgggctcg cgggcgacgt cggtgccgcc gtcgaccgtg gcgtggcaat
    26341 ggtcgagcag ggcgcggcgc tgctggacgt cggcgcggaa tcgacccgcc ccggggcaga
    26401 gccggttccg gacgcggatg agctcgcccg cgtgctgccc gtgatcgagg ccctggcgcg
    26461 acgcgtggac gtgccgattt cgatcgacac ttccaagccc gatgtgatgc gggccgccgt
    26521 cggtgcgggc gcggggctga tcaacgacgt gtacgcacta cgctgcgagg gggcgctcga
    26581 agcggccgcc acgctcaagg tgccggtatg cctgatgcac atgcaaggcg agccgcgcac
    26641 gatgcaggag gatccgcact acgacgacgt ggtcggcgag gtgcaccggt tcctcgcaga
    26701 gcgcgtgttc gcctgcgaga tggccgggat cgacaagcgc cgggtgctgg tcgatccggg
    26761 cttcgggttc ggcaagacac tggaccacaa tctcgcgttg ctgcgcgggc tggagcgctt
    26821 ccgtgatctc gccgcggggg tattcgtcgg gctctcgcgc aaggcgatga tcggcaccct
    26881 gaccggccgc ggcaggggcg tggagcgggc ggcgggctcg gccgcggct
//
LOCUS       DASSNH010000246         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1036949, whole genome shotgun sequence.
ACCESSION   DASSNH010000246 DASSNH010000000
VERSION     DASSNH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036949"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFK00_09115"
     CDS             complement(<1..479)
                     /locus_tag="VFK00_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7593149.1"
                     /translation="MQPKRNVMALALISAGIGFASCTNIAFAATAGSGGGQDQNQQQT
                     DTSQAAQPAQTDAQKKQQQQEKQKAKAVTLNAVQVTGFASSVQNSIEIKRQSDSIVEA
                     VSAEQIGKLPGTSIADTLGRLPGLAVQTVSGRPQVLTIHGLGPDFSTALVNGNQQVST
                     "
     gene            961..2628
                     /locus_tag="VFK00_09120"
     CDS             961..2628
                     /locus_tag="VFK00_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase family protein"
                     /protein_id="HET7593150.1"
                     /translation="MSDVPWWRGAVIYQIYPRSFLDTNGDGVGDLPGIIERLDYVASL
                     GVDAVWIAPFFKSPMADFGYDIADYRQVDPLFGTLGDFDSLLDKAHALGLKVMIDQVL
                     SHTSAEHDWFRESRQSRHNPKADWYVWADPRGDGTPPNNWLSIFGGPAWQWEPRRQQY
                     YLHNFLAEQPDLNFHHPDVRAAVLDNVEFWLDRGVDGLRLDAINFCFHDPELRDNPAK
                     PADQRIGRGFSPDNPYAHQYHFYNNTRPENLAFLERLRALLDRYPGVATLGEISSEDS
                     LATIAEYTSGRRLHMGYSFELLTDDFSAAWIRETVCRLDAKMQEGWPCWAISNHDVER
                     VLSRWGASRPAAALAKLLTAMLCSLRGAVCVYQGEELGLTEAEVPYEALRDPFGIAFW
                     PQFKGRDGCRTPMPWSGDMHAGFTTGEPWLPVPDEHLALAVARQETDPESILHGFRTF
                     LRWRRSHPALCTGTIRFLHTAEPVLAFTRHADDEVLLVAFNLSDRAAELTLPIAGASS
                     LLHGHGLREGALDGNHLHLPRYGALFARLEMVDAQPVFAATAMGAAS"
     gene            2625..4049
                     /locus_tag="VFK00_09125"
     CDS             2625..4049
                     /locus_tag="VFK00_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HET7593151.1"
                     /translation="MNESISAPENLQGRTGLRVILIAAAAALGGFLFGFDTAVINGAV
                     DSLRAAFGLSAALTGFAVSIALLGSALGAWYAGRLADRYGRVRTMQVAAILLAAGAVG
                     AGLSGSVAWLMAWRVIAGIGVGVASVVAPAYIAEISPASIRGRLGSMQQLAIVLGIFV
                     ALLSDAALAGAAGGAAETFWFGLGAWRWMFIVGVVPAVVYGVLAFSVPESPRHLVAGG
                     RNEEALGVLRRVLALGSEAALQKKLSDIQLSLKREHKPAWSDLVGGSAGLLPIVWIGI
                     VLSVFQQFVGINVIFYYSTTLWNSVGFSESNSFMLTVITSIVNVLVTLVAIALIDRIG
                     RKALLVIGSSGMLVTLAVMAACFAQATGSGTAVSLPGAWGPVALVAANLYVVFFGVSW
                     GPVVWVLLGEMFPNRIRAMALAVAAAAQWLANFAITSSFPPMAQFSLPFSYGLYAFFA
                     LLSLLFVLRFVRETRGVELEDMPG"
     gene            4156..5199
                     /locus_tag="VFK00_09130"
     CDS             4156..5199
                     /locus_tag="VFK00_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7593152.1"
                     /translation="MGNKKRITSFDIAELAGVSQATVSRALRGSARVSEETRQRVAAA
                     AASLHYTVDKNASSLRSKTSGTLALLFFEDPTPDDSRINPFFLSMLGSITRECARQGY
                     DLLISFQELARDWHADFAESKKADGIILLGYGDYLAHASRLEKLCAQGTRIVCWGAVL
                     PAQPCVTIGCDNFNGGRSVTGHLLGQGCRRIAFLGDASNHFPEFQERYRGHLHALRTH
                     GIAVDEALQVDAESSQYSGRRAVEALLARGARFDAIFAASDLIAIGALGALSAHGIEV
                     PDDVAIAGFDDIPMASFVNPPLTTVLQDTALAGGILVDSLLRLIRGEPVASRMLPMKL
                     VVRDSSRRQTACA"
     gene            5279..>6120
                     /locus_tag="VFK00_09135"
     CDS             5279..>6120
                     /locus_tag="VFK00_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7593153.1"
                     /translation="MEKLALIGDIGGTNARFALADLAAPEPRIVEEHTFTNSGFRSLG
                     EAAQHYLEDLGMHPKRAALAVASPITGDEITLTNRDWSFRRSVLQEALGLDELRLIND
                     FAAIAWAGPHLAEGDRMAVHGTIVTPPAGPVTLIGAGTGLGVALLTGSFADGWHVVPT
                     EGGHASFAPIDEEERALAAWLRVRHGRISNERLLSGHGLSNIDAWLRGLEPVAAPAQQ
                     PGSRRSKEVVDAALSGNDPDAERALHRFCRIYGAVAGDAALLHGASTVLIAGGIVLHF
                     LDFFR"
BASE COUNT          901 a         2082 c         2062 g         1075 t
ORIGIN      
        1 gtactgacct gctggttgcc gttgaccagc gcggtgctga agtcgggacc gaggccgtgg
       61 atcgtgagta cttgcggacg gccgctgacg gtctggaccg cgaggcccgg caggcgaccc
      121 agggtgtcgg cgatgctggt gccgggaagc ttgccgatct gctcggccga aacggcctcg
      181 acgatcgagt cggactggcg cttgatctcg atcgagttct ggacgctgct cgcgaatccg
      241 gtgacctgga ccgcgttcag ggtcacggct tttgccttct gcttttcctg ctgctgctgc
      301 tttttctggg cgtcggtctg ggccggctgc gctgcctggg aggtatcggt ctgctgctgg
      361 ttctggtcct gccctccgcc gctgccggcg gtggccgcaa aggcgatgtt ggtgcaggag
      421 gcgaagccga tcccggcaga gatcagcgcc aatgccatga cattccgttt cggttgcatg
      481 tgtctttccc cagcaggtta ttggtgtctt gtgtgctcgc gcttcccgac aaagcacgcg
      541 cagtccgccc atgggcgcgg ccggtgccga tggtaggggg cgagttcccg gccgcacgct
      601 tcgtgcatac gtattcatgc cgcgccggaa gcatcgatac tgcattgcag caatgcagtg
      661 gggaccgggc accggggacc agggagcggg agagcgcgaa acgctcgcac cttccggaca
      721 cgcgctccca agagccgggt ggagaggagc tacccatgcg aattcgttga ttcaccttcg
      781 cccgcttttg gtctccggtc tccggtcccc ggtccctggg ccctcgctcc tggatgacgc
      841 acgtgcagca tccatgaata cgtatgcatg cccgggcggg acggcggcgt ccggacgtta
      901 agcttggcgc cgatcacagc cgcccgggca tcgcgccggg cggcgttgcc tggggcctgt
      961 atgtcagacg ttccctggtg gcggggcgcc gtcatctacc agatctatcc gcgcagcttt
     1021 ctcgacacca atggcgacgg ggtcggcgac ttgcccggca tcatcgagcg gctcgactac
     1081 gtggccagcc tcggcgtgga cgccgtctgg atcgcgccgt tcttcaagtc gccgatggcg
     1141 gacttcggct acgacatcgc cgattaccgc caggtcgatc cgctgttcgg aacgctcggc
     1201 gacttcgaca gcctgctcga caaggcgcac gcgctgggcc tgaaggtcat gatcgaccag
     1261 gtgctcagcc acacctcggc cgaacacgac tggtttcgcg aaagccggca aagccgccac
     1321 aacccaaagg ccgactggta tgtctgggcc gatccccgcg gggacggcac gccgcccaac
     1381 aactggctgt cgattttcgg gggtccggcc tggcaatggg aaccgcgccg ccagcagtac
     1441 tacctgcaca acttcctcgc cgagcagccg gacctcaatt tccaccaccc ggacgtgcgc
     1501 gcggcggtgc tggacaacgt ggagttctgg ctcgaccgcg gcgtcgacgg cctgcgcctc
     1561 gacgcgatca acttctgctt ccatgatccg gagttgcgcg acaatccggc caagcccgcg
     1621 gaccagcgga tcgggcgcgg cttcagcccc gacaatccct acgcgcacca gtaccacttc
     1681 tacaacaaca cccggccgga gaacctggcg ttcctggaac gcctccgcgc cctcctggac
     1741 cgttatcccg gcgtggccac cctcggcgag atttcctcgg aagactcgct cgcgacgatc
     1801 gccgaataca ccagcggccg gcgcctccac atgggctaca gcttcgaact gctcacggac
     1861 gacttcagcg ccgcgtggat ccgcgagacc gtgtgccggc tcgacgcgaa gatgcaggaa
     1921 ggctggccgt gctgggcgat ttccaaccac gacgtcgagc gcgtgctctc gcgctggggc
     1981 gcgagccggc cggcggcggc actcgccaaa ctgttgacgg cgatgctgtg ctcgctgcgc
     2041 ggcgcggtct gcgtgtacca gggcgaggaa ctcggcctca ccgaggccga ggtgccctac
     2101 gaggcgctgc gcgacccgtt cggcatcgcg ttctggccgc aattcaaggg gcgcgacggc
     2161 tgccgcaccc ccatgccctg gagcggcgac atgcatgccg gcttcaccac cggcgaaccg
     2221 tggctgccgg tgccggacga acaccttgca ctggcggtgg cccgccagga aaccgatccc
     2281 gaatccatcc tgcacggctt ccgcaccttc ctgcgctggc gcaggtccca tcccgccctg
     2341 tgcacgggca cgatccgttt cctgcatact gccgaacccg tgctggcctt cacgcgtcac
     2401 gccgacgacg aggtgctgct ggtggcgttc aatctttccg atcgagcggc cgaactgacg
     2461 ttgccgattg ccggcgcttc cagcctcctg cacgggcacg gcttgcgcga aggcgcgctg
     2521 gacggcaacc acctgcacct gccgcgctac ggagcgctgt tcgcgcggct cgagatggtc
     2581 gatgcgcagc ccgttttcgc cgccaccgcc atgggagccg cgtcatgaac gaatcgataa
     2641 gcgcaccgga gaacctgcaa ggccgcactg gactgcgggt aatcctcatc gccgccgccg
     2701 ccgcactcgg cggtttcctg ttcgggttcg acaccgccgt gatcaacggc gcggtcgatt
     2761 cgctgcgcgc cgcgttcgga ttgagcgccg ccctgaccgg attcgccgtg tccatcgccc
     2821 tgctcgggtc cgcgctcggc gcatggtacg caggacgact cgcggaccgt tacggacgcg
     2881 tgcgcacgat gcaggtcgcc gcgatattgc tggccgccgg cgctgtcggc gccggccttt
     2941 ccggttcggt ggcatggctg atggcgtggc gcgtcatcgc cggcatcggc gtcggcgtgg
     3001 cctcggtggt agcgcccgcc tacatcgccg agatatcgcc ggcgtcgata cgagggcggc
     3061 tcggctcgat gcagcaattg gcgatcgtgc tcgggatatt cgtggccctc ttgagcgacg
     3121 cggcgctggc cggtgccgcg gggggcgccg ccgaaacgtt ctggttcggc ctcggcgcct
     3181 ggcgctggat gttcatcgtg ggcgtggttc ccgcggtggt ctacggcgtc ttggcgttca
     3241 gcgttcccga atcgccgcgg cacctggtcg ccggtggtcg caacgaggaa gcactgggcg
     3301 tgctgcggcg ggtgctggca ctcggttcgg aagcggcgct ccagaaaaag ctgagcgaca
     3361 tccagctcag cctgaagcgc gagcacaaac cggcctggag cgacctggtc ggggggagcg
     3421 ccggattgct gccgatcgtg tggatcggca tcgtgctgtc ggtgttccag cagttcgtcg
     3481 gcatcaacgt gatcttctac tactccacga cgctgtggaa ttccgtcggc ttcagcgaat
     3541 ccaactcctt catgctcacg gtcatcacct cgatcgtgaa cgtgctggtg acgctggtgg
     3601 ccatcgcgct gatcgaccgg atcgggcgca aggcgctgct ggtgatcggc tcgtcgggca
     3661 tgctggttac cctcgccgtg atggcggcgt gcttcgccca ggccaccggc agcggcacgg
     3721 cggtgagcct gcctggcgca tggggtccgg tcgcactggt cgcggccaac ctgtacgtgg
     3781 tcttcttcgg ggtgagctgg gggccggtcg tgtgggtgct gctgggcgag atgttcccca
     3841 accggatccg cgcgatggcg ctggcggtcg ccgcggcggc gcaatggctg gccaatttcg
     3901 cgattaccag cagtttcccg ccgatggccc agttcagcct gccgttctcg tacggcctgt
     3961 acgcgttctt tgccttgctg tcgttgctgt tcgtgctccg cttcgtgcgc gagacccgtg
     4021 gcgtggagct ggaagacatg cctggctgag gtgccgagca acggggcgcc cgcgacgcca
     4081 cgcagtgcac ccgcgcactg ctacgattcg cgcaatcgcg gccgccgggc cgcgttgcag
     4141 gggttgcgtc tcggggtggg caacaagaag cgcatcacgt cgttcgacat cgccgaactg
     4201 gccggcgtct cgcaggcgac ggtttcgcgc gccttgcgcg gcagcgcccg ggtcagcgag
     4261 gaaacccgcc agcgggtggc ggcggccgcg gcctcgttgc actacacggt cgacaagaac
     4321 gcgtccagcc tgcgcagcaa gacgtccggc acgctcgccc tgttgttctt cgaagacccg
     4381 accccggacg actcgcggat caatccgttc ttcctctcga tgctcggctc gatcacccgc
     4441 gagtgcgcgc gccagggata cgacctgctg atctcgttcc aggaactcgc gcgcgactgg
     4501 cacgccgatt tcgccgagag caagaaggcc gacggcatca tcctgctcgg ctacggcgat
     4561 tacctcgccc acgccagccg cctcgagaag ttgtgcgccc agggcacccg catcgtgtgc
     4621 tggggcgcgg tgctgcccgc gcaaccctgc gtcacgatcg gctgcgacaa cttcaacggc
     4681 ggccgctcgg tcaccggaca cctgctgggc cagggttgcc ggcgcatcgc gttcctcggc
     4741 gacgcgtcga accacttccc ggaattccag gagcgttacc gcggccacct gcacgcgctg
     4801 cgcacccacg gcatcgccgt cgacgaggcg ctgcaggtcg atgcggaaag ctcgcagtat
     4861 tccgggcggc gggccgtcga ggcgttgctc gcgcgcggcg cccgcttcga cgcgatcttc
     4921 gccgccagcg acctgatcgc gatcggggcg ctgggcgcgc tttcggcgca cggcatcgag
     4981 gtgcccgacg acgtggcgat cgccggcttc gacgacatcc cgatggcgag cttcgtcaat
     5041 cctcccttga cgaccgtgct gcaggacacc gcgctcgccg gcggaatact ggtcgacagc
     5101 ctgttgcgcc tgatccgcgg cgagcccgtg gcgagccgga tgctgccgat gaaactggtg
     5161 gtgcgcgact ccagtcgccg gcaaaccgcc tgcgcgtaaa tggcgcgaaa ccgcaacccg
     5221 ccgcatgaca tgcgcatcgg ttgttgctta tgctgtcgat ctgtcaccgg agcacatcat
     5281 ggagaagctg gccctcatcg gcgacatcgg cggcaccaac gcgcgcttcg ccctggccga
     5341 tctcgcggcg ccggaaccgc ggatcgtgga ggaacacacc ttcaccaaca gcggcttccg
     5401 gagcctgggt gaagccgcgc agcattacct ggaagacctc gggatgcacc cgaagcgggc
     5461 ggcgttggcg gtcgcctcgc cgatcacggg tgacgagatc acgctcacga accgcgactg
     5521 gtcgttccgg cggagcgtgc tgcaagaagc cttggggctc gacgagttgc ggctcatcaa
     5581 cgatttcgcg gcgatcgcct gggccgggcc gcacctggcc gaaggcgacc gcatggcggt
     5641 gcacggcacg atcgtgacgc cgcccgcggg gccggtgacc ctgatcggcg ccggcaccgg
     5701 gctcggcgtc gcgctgctca cgggatcgtt cgcggacggt tggcacgtgg tgccgacgga
     5761 gggaggccac gccagcttcg cgcccatcga cgaggaggag cgcgcactcg ccgcctggct
     5821 acgggttcgg cacggacgca tttccaacga acggctcttg agcggccacg gcctttccaa
     5881 catcgatgcg tggctgcgcg gcctcgaacc cgttgcggcg ccggcgcagc aacccggctc
     5941 gcgtcgctcc aaggaagttg tcgatgcggc gttgtcggga aatgacccgg acgccgagcg
     6001 tgccctgcac cgcttctgcc ggatctacgg cgccgtggct ggcgatgccg ccctgctgca
     6061 cggcgccagc acggtactga tcgccggcgg catcgtgctg cacttcctcg atttcttccg
//
LOCUS       DASSNH010000247         7510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1541344, whole genome shotgun sequence.
ACCESSION   DASSNH010000247 DASSNH010000000
VERSION     DASSNH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541344"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1696
                     /gene="rpsA"
                     /locus_tag="VFK00_09140"
     CDS             20..1696
                     /gene="rpsA"
                     /locus_tag="VFK00_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7593154.1"
                     /translation="MTESFAELFEQSQSLAKLKPGSIVSGIVVEIRPDVVVVNAGLKS
                     EGIVPIEQFKSERGDLEVEVGDEVKVALDAIENGFGETMLSREKAKRSMVWDELEEAF
                     SNEAAVTGRINGKVKGGFTVDIQDVRAFLPGSLVDVRPVRDPAYLEGKDLEFKIIKLD
                     RKRNNVVVSRRAIVEEEHSEEREQLLEKLQEGAILHGVVKNLTDYGAFVDLGGIDGLL
                     HITDMAWKRVRHPSEVVNVGDELDVRVLKFDRERNRVSLGLKQLGDDPWVSITRRYPS
                     GTRLFGKVSNVTDYGCFVELEPGVEGLVHVSEMDWTNKNVNPAKVVQVGDEVEVMVLD
                     VDEERRRISLGMKQTQSNPWEAFAATYKKGDKVQGAIKSITDFGIFVGLEGGIDGLVH
                     LSDISWQGNGEELVRDFKKGEEVDAVVLAVDPERERISLGIKQLEQDPFGQFMAGHPR
                     GAILTGTVKEVDAKGAVVDLADGIEGYLGARDFSKDRIDDLSQHLKVGDQVEAKFIGM
                     DRKGRMLQLSIRAKDEAEMAETLSEYQSQHPSQGTTKLGALLKEQLNKPE"
     gene            1830..2126
                     /gene="ihfB"
                     /locus_tag="VFK00_09145"
     CDS             1830..2126
                     /gene="ihfB"
                     /locus_tag="VFK00_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET7593155.1"
                     /translation="MTKSELISALADRQKHLAFADVELAVRNVIEQMSVALASGDRIE
                     IRGFGSFSLHYRPPRTGRNPKTGTAVALPGKYVPHFKPGKELRHRVNEGRDRAP"
     gene            2319..3500
                     /gene="lapB"
                     /locus_tag="VFK00_09150"
     CDS             2319..3500
                     /gene="lapB"
                     /locus_tag="VFK00_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HET7593156.1"
                     /translation="MLPVDPLLLLLLLVPLAAWLGWIIGRHASARRSGARVTELSSNY
                     FRGLNYLLNEQPDKAIEVFMKLAEFNRDTVETHLALGNLFRRRGEVDRAIRVHQHLVS
                     RSNLSEAERTVALLELGEDYMRAGLLDRAEALFDDLVAMDAHAPSALRHLIAIYQHER
                     DWHKAIGHARRLEKMTGESQAATIAQFQCELAEQSRQHGARTEARAYLEEAFASQPGC
                     VRGYMISGHLHAEEGRWNEAVLAFEQAVDADVDFVPEILPPLLDAYAHSGEMDRAEKF
                     LGQVIERYHGITPVLALTDLYRKREGERAAIDFLTGQLRQRPSVRGMMALIDATMGRA
                     EGEARENFLILRDLTRKLLEGRAMYRCNHCGFGAKAHHWQCPSCKSWGTVRPIRSVVS
                     E"
     gene            3718..4752
                     /locus_tag="VFK00_09155"
     CDS             3718..4752
                     /locus_tag="VFK00_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 4"
                     /protein_id="HET7593157.1"
                     /translation="MDPLISAVCGFVVSLLMTAATLRYARRRGLIDLPGKRRSHTQPT
                     PRGGGIGIVAAILLPGVPAWGVLDPAAAWIEPAAFAAAVLAVALIGWRDDHAPLSVLP
                     RLAVHAVAALLVGAAVLVQGGWLATIGWVALVPLAIVLIGSINAHNFMDGIDGILGQQ
                     GLFVLLGYGLLATGAGHAGLAALAFAAAAGCLGFLLFNFPPARIFMGDVGSGALGFAI
                     GAVAVFLIQRDPTLLWACVILPSAFLVDSGLTLARRMSAGQRWYAPHRQHLYQWLVRV
                     NWSHARTDVAYMIWNLAVVTPLAWLAACWPARGIWFFVAAYGLAIVAWHLGKRACLAS
                     ARKRFVHETA"
     gene            4739..6649
                     /locus_tag="VFK00_09160"
     CDS             4739..6649
                     /locus_tag="VFK00_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET7593158.1"
                     /translation="MKLRDLAGRIHPRTAVVAHDLGVTILAWWLANTVRYAMLPYGQR
                     LDLLDWKTLIVLGVQAAIFYWTGLYKGLWRFASVPDLWNILRAVVAGAVGIYLALFLY
                     DRMAGISRSVLLIYPVLLAIFLGAPRLAYRYWKDSQLDLFHARHSHRVLVIGAGRAGE
                     ALVRDLRRDSRYIPAGFIDDDVSLRGSRLQGVPVLGGTGRLVEMARRTGAEMLLIAMP
                     SADKQQMRQVVELCEQTGLPFRTVPRIDDVVSGRAQFNELKEVAIEDLLGREAVKLEW
                     NAIRRSLTGKRVLVTGGGGSIGSELCRQIARLGASELTILELSEYNLYRIEQELHAQY
                     PELLLRCLVGDCGDPATCANAFDLGKPQRVFHAAAYKHVPLLQNQLRETFRNNALATR
                     TVAEEAVRRGVESFVLISTDKAVNPTSVMGACKRVAEIACQVLAEGHATRFVTVRFGN
                     VLDSAGSVVPLFRRQIAAGGPVTVTHPEISRYFMTIPEACQLILQAMAMGRGGETFAL
                     DMGEPIRIRDLAEQMIRLAGKQPGRDISILYTGLRPGEKLFEELFHPLENYHSTSHPK
                     IFEATPRQQSGAMVMAQLARASDAVVAFDEEALRRCMSALLPTYRWETGNAAQTAEVL
                     PMQRGSGSDTAA"
     gene            6876..>7510
                     /locus_tag="VFK00_09165"
     CDS             6876..>7510
                     /locus_tag="VFK00_09165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET7593159.1"
                     /translation="MTRLRKVVFPVAGLGTRFLPATKVVAKEMLPVLDRPLIQYAVDE
                     AVDAGADTLVFVTNRYKHAIADYFDSAYELEETLERAGKHELLEMVQGLLPPHVRCVY
                     VTQEQALGLGHAVLCAKPVIGDEPFGVILPDDLIVNGGAGALRQMAERAADGRTGVLA
                     VEEVPHSDTGKYGIVDVGSDGDRIRHMVEKPKPEDAPSNLGVVGRYVLPAR"
BASE COUNT         1285 a         2453 c         2508 g         1264 t
ORIGIN      
        1 ttcccaacct ggaatcccca tgaccgaaag ttttgccgag ctgtttgaac agagccagtc
       61 gctggccaaa ttgaaacccg gatcgatcgt atccggcatc gtcgtcgaaa tccgccccga
      121 tgtggtggtg gtgaacgccg gactgaaatc ggagggcatt gtccccatcg aacagttcaa
      181 gagcgaacgc ggcgacctgg aagtcgaggt gggcgacgaa gtgaaggtcg cactcgacgc
      241 gatcgaaaac ggcttcggcg aaacgatgct ttcgcgcgag aaggccaagc gttccatggt
      301 gtgggacgag ctggaagagg cgttctcgaa cgaggcggcc gtcaccggcc gcatcaacgg
      361 caaggtcaag ggtggcttca ccgtcgacat ccaggacgtc cgcgcgttcc tgccgggttc
      421 gctggtggac gtgcgcccgg tgcgcgaccc ggcctacctc gagggcaagg acctcgaatt
      481 caagatcatc aagctcgacc gcaagcgcaa caacgtggtc gtcagccgcc gcgcgatcgt
      541 cgaggaagag cattccgagg agcgcgaaca gctgctcgag aagctgcagg agggcgcgat
      601 cctgcacggc gtggtcaaga acctcaccga ctacggcgcg ttcgtggacc tcggtggcat
      661 cgacggcctg ctgcacatca ccgacatggc gtggaagcgc gtgcgccatc cgtcggaagt
      721 cgtcaacgtg ggcgacgagc tggacgtgcg cgtgctgaag ttcgatcgcg agcgcaaccg
      781 cgtctcgctg ggcctgaaac agctgggcga cgatccgtgg gtcagcatca cccgccgcta
      841 tccgtcgggc acccgcctgt tcggcaaggt ctcgaacgtc accgactacg gctgcttcgt
      901 ggagctggag ccgggcgtcg aaggcctggt gcacgtgtcc gagatggact ggaccaacaa
      961 gaacgtcaac ccggccaagg tggtgcaggt cggcgacgaa gtcgaagtga tggtgctcga
     1021 cgtggacgag gagcgccgcc gcatttcgct gggcatgaag cagacccagt ccaacccgtg
     1081 ggaagccttc gcggccacct acaagaaggg cgacaaggtc cagggcgcga tcaagtcgat
     1141 caccgacttc ggcatcttcg tggggctcga aggcggcatc gatggcctgg tgcacctgtc
     1201 cgacatttcc tggcagggca acggcgagga actggtccgc gacttcaaga agggcgagga
     1261 agtcgacgcc gtggtgctgg cggtcgatcc ggagcgcgaa cgcatcagcc tcggcatcaa
     1321 gcaactcgag caggatccgt tcggccagtt catggctggg cacccgcgcg gcgcgatcct
     1381 caccggcacg gtgaaggaag tcgacgccaa gggcgcggtg gtcgaccttg cagatggcat
     1441 cgagggttac ctcggcgccc gcgacttctc caaggaccgc atcgacgacc tcagccagca
     1501 cttgaaggtg ggcgaccagg tcgaggccaa gttcatcggc atggatcgca agggccgcat
     1561 gctgcagctc tcgatccggg ccaaggacga ggccgagatg gccgaaacgc tgtccgagta
     1621 ccagagccag catccgagcc aaggcaccac caagctcggg gctctcctga aagaacagtt
     1681 gaataagccc gagtaagtct atataagggc ttgcgttttg atgctgcacg gtggtgccgc
     1741 cccggagccg gggcggcacc acgaacatgg gggtcgtccc tgctcgatcc gcacccgcct
     1801 tcctgccagg tagccgcacg ttgccatcga tgaccaaatc cgaattgatc tccgcgctcg
     1861 cggatcgtca gaagcatttg gcgttcgccg atgtggagct tgcggtgcgc aacgtgatcg
     1921 aacagatgag cgtcgcgctg gcgagcggcg atcgcatcga gatccgcggg ttcggaagct
     1981 tttcgctgca ttaccgtccg ccccggaccg gccgcaatcc caagaccggg acggcagtgg
     2041 cgctgcccgg caagtacgtt ccccacttca agcccggcaa ggaattgcgg caccgcgtca
     2101 acgaaggccg cgaccgcgcg ccgtgaggcc ggccggtccg ccgcgtcgcg ttttcttgca
     2161 atcgtccctg atcgcagctt cgttcgatcc ctctctgcgt cgatggcctt tcggttgaca
     2221 cacaccagaa tggccttccc tggcctgact tttaacaaca gccgcttcga gcagggcgtc
     2281 gaaaactgac gggcgcggca tcggagattt tggtgagcct gctgcccgtc gatccgctgc
     2341 tgttgttgct gctgctggtg ccgctcgccg catggctggg ctggatcatc ggccggcatg
     2401 ccagcgcacg ccgctcgggc gcacgcgtca ccgagctgtc ctcgaactac ttccgcggcc
     2461 tcaactacct gctcaacgag cagccggaca aggccatcga ggtgttcatg aagctggccg
     2521 agttcaaccg cgacacggtg gaaacccacc tcgcgctcgg caacctgttc cgccgccgcg
     2581 gcgaagtcga ccgcgcgatc cgggtccacc agcacctcgt cagccgttcc aatctttccg
     2641 aagccgagcg caccgtcgcg ctgctggaac tgggcgagga ctacatgcgc gcgggcctgc
     2701 tcgaccgcgc cgaagcgctg ttcgacgacc ttgtcgcgat ggacgcgcac gcaccatcgg
     2761 cgctcaggca cctgatcgcg atctaccagc acgagcgcga ctggcacaag gcgattggac
     2821 atgcgcgccg gctggagaag atgaccggcg aatcccaggc cgcgacgatc gcccagttcc
     2881 agtgcgagct cgccgagcag tcgcgccagc acggcgcgcg caccgaggcc cgcgcctacc
     2941 tcgaggaagc cttcgccagc cagccaggat gcgtgcgcgg ttacatgatc agtggccacc
     3001 tccacgccga ggaaggccgc tggaacgaag ccgtgctcgc attcgagcaa gcagtcgacg
     3061 ccgacgtcga tttcgttccg gagatcctgc cgccgttgct cgacgcgtac gcgcattcag
     3121 gagaaatgga ccgcgccgag aagttcctgg gccaggtgat cgagcgctac cacggcatca
     3181 caccggtgct ggcattgacc gacctctacc gcaagcgcga gggcgagcgc gccgcgatcg
     3241 acttcctgac cgggcaattg cgccagcggc cctcggtgcg cgggatgatg gcgctgatcg
     3301 atgccacgat gggccgcgcc gaaggcgagg cgcgcgagaa cttcctgatc ctgcgtgacc
     3361 tcacccgcaa gctgctcgag gggcgcgcga tgtaccgctg caaccactgc ggcttcggcg
     3421 ccaaggccca ccactggcag tgcccgagct gcaagagctg ggggacggtg cggccgatcc
     3481 gcagcgtcgt cagcgagtag gggctgcccg ggcacgggcg ccaaagcgag agcctttgac
     3541 gcggatcaac gcggacatag gcggataaag gcggatgcaa ggagctggtg tggaggtgct
     3601 tgccaactct gatctgctgc tgctccgccc ccgcgcatcc gcgtagatcc gcgtcaacaa
     3661 atgccttgtg aggaaaccga aaatggtccc cgcgtgcgac ctgaaggaaa ttgacgcatg
     3721 gaccccctga tcagcgcggt gtgcggcttc gtggtatccc tgctgatgac cgccgccacg
     3781 ctgcgctacg cgcggcgtcg cgggctcatc gatttgccgg gcaagcgccg ttcgcacacg
     3841 cagcccacgc cgcgcggggg cggcatcggc atcgtcgcgg cgatcctcct gcccggtgtg
     3901 ccggcttggg gcgtcctcga tccggcggcg gcctggatcg agccggcagc cttcgcggcg
     3961 gccgtgctag cggtcgcgct gatcgggtgg cgcgacgacc atgcgccgct ttcggtgctg
     4021 ccgcgcctgg ccgtccatgc ggtggcggcg ctgctggtcg gcgcggccgt actggtgcag
     4081 ggcggctggt tggcgacgat cggctgggtg gcgctggtcc cgctggcgat cgtgctgatc
     4141 ggctccatca atgcgcacaa cttcatggac ggcatcgacg ggatcctcgg ccagcaaggc
     4201 ttgttcgtgc tgctgggcta tggtttgctc gcgaccggcg ccggccacgc cgggctcgcc
     4261 gcgctggcgt tcgccgcggc ggcggggtgc ctcggcttcc tgctgttcaa cttcccgccc
     4321 gcccgtattt tcatgggcga cgtcggatcg ggcgccttgg gattcgcgat cggtgccgtg
     4381 gccgtattcc tcatccagcg cgacccgacg ctgctctggg cctgcgtgat cctgccctcg
     4441 gccttcctgg tggacagcgg cctgaccctc gcgcgacgca tgtcggccgg acaacgttgg
     4501 tatgcgccgc accgccagca cctctaccaa tggcttgtac gggtaaactg gagccatgcg
     4561 cgcacggacg tcgcctacat gatctggaac cttgccgtcg tcacgccgct ggcatggctg
     4621 gcggcgtgct ggcctgcgcg cgggatctgg tttttcgtgg cggcgtatgg cctggcgatc
     4681 gtcgcctggc acctcggcaa gcgcgcctgc ctcgcttcgg cgcgcaagag gttcgtgcat
     4741 gaaactgcgt gatctcgcgg gcaggatcca tccgcgcacc gctgtcgtgg cccacgacct
     4801 cggcgtgacg atcctcgcat ggtggctcgc caacacggtg cgctacgcga tgctgccgta
     4861 cggacagcgg ctcgacctgc tcgactggaa gaccctgatc gtgttgggcg tgcaggcggc
     4921 gatcttctac tggaccggcc tgtacaaggg actgtggcgt ttcgcgagcg tgccggatct
     4981 ctggaacatc ctgcgtgcgg tggtggccgg tgcggtcggc atctaccttg cgctgttcct
     5041 gtacgaccgc atggccggga tttcgcgttc ggtgctgctg atctacccgg tgttgctggc
     5101 gatcttcctg ggtgcccccc ggctcgcgta ccgctactgg aaggacagcc aactcgacct
     5161 gttccatgcg aggcacagcc atcgggtgtt ggtgatcggc gcgggccgcg ccggcgaggc
     5221 gctggtgcgc gacctgcgcc gcgactcgcg ctacatcccg gcgggcttca tcgacgacga
     5281 cgtgtcattg cgcggttccc gcctgcaagg cgtgccggtg ctgggcggca ccggccggct
     5341 ggtggagatg gcgcgccgca cgggcgccga gatgctgttg atcgcgatgc cgagcgcgga
     5401 caagcagcag atgcggcagg tggtcgagtt gtgcgagcag accggcttgc cgttccgcac
     5461 cgtgccgcga atcgacgacg tcgtgtccgg ccgcgcgcaa ttcaacgaac tcaaggaagt
     5521 cgcgatcgag gacctgctcg gccgcgaggc cgtcaagctg gagtggaatg ccatccggcg
     5581 cagcctcacc ggcaagcgcg ttctggtcac cggcggcggc ggttcgatcg gttcggaact
     5641 gtgccgccag atcgcccgcc tcggtgcgtc cgaactcacg atcctcgagc tctcggagta
     5701 caacctctac cggatcgagc aggagctgca cgcgcagtat ccggagctgt tgttgcgctg
     5761 cctggtcggt gactgcggcg atcccgcgac ctgcgccaac gcgttcgacc tcggcaaacc
     5821 gcaacgcgtg ttccacgcgg ccgcctacaa gcacgtgccg ctgctgcaga accagttgcg
     5881 cgagacgttc cgcaacaacg cgctggcgac gcgtacggtc gccgaagaag ccgtccgccg
     5941 cggcgtcgaa agcttcgtgc tgatctcgac cgacaaggcg gtcaatccca ccagcgtgat
     6001 gggtgcctgc aaacgcgtcg ccgaaatcgc ctgccaggtg ctggcggaag gccacgccac
     6061 gcgtttcgtc acggtccggt tcggcaacgt gctcgattcg gcgggctcgg tggtgccgtt
     6121 gttccgcagg cagatcgcgg ccggcgggcc ggtcaccgtc acccacccgg agatctcgcg
     6181 ctacttcatg accattccgg aagcctgcca gttgatcctg caggcgatgg cgatggggcg
     6241 gggcggcgaa acctttgcgc tcgacatggg cgaaccgatc cgcatccgcg atctcgccga
     6301 gcaaatgatc cggctcgcgg gcaagcaacc cgggcgcgac atttcgatcc tctataccgg
     6361 gctccggccg ggcgaaaaac tgttcgagga actgttccat ccgctcgaga actaccacag
     6421 cacttcgcat cccaagatct tcgaggcgac gccccggcag cagtcgggcg cgatggtgat
     6481 ggcgcagctc gcgcgggcat ccgacgcggt ggtggccttc gacgaagagg cgctgcgccg
     6541 ttgcatgagt gcactgctgc cgacctatcg ctgggaaacc ggcaacgccg cgcagaccgc
     6601 ggaggtgctg ccgatgcaac gtggcagtgg aagcgacacg gcggcataag ttgtgcgatc
     6661 gtccctgatc gctgcttcgt cggaggcgcc gattacaatt gcttctgcag gcgtccatcc
     6721 atggcgcggg ggctgggtcc gccatccctg gccggtcgct cgggcgggca ggcgatgtca
     6781 ccggcatcgc cggagcgccc gtcctcgccc cgacttttca ccaacaaccg cttcggacga
     6841 gcggatttgg aaatcgcaac aatcaaggag aagaagtgac acgattgcgc aaagtggtat
     6901 ttcccgtggc cgggctcggc acgcgtttcc tgcccgcgac caaggtggtt gccaaggaaa
     6961 tgctgccggt actggaccgt cccctgatcc agtacgcggt cgacgaggcg gtcgacgccg
     7021 gcgccgacac cctggtattc gtcaccaacc gctacaagca cgcgatcgcc gactatttcg
     7081 actccgccta cgagctcgag gaaaccctcg aacgcgcggg gaagcacgaa ctcctcgaaa
     7141 tggtgcaggg cctgctgccg ccgcacgtgc gttgcgtata cgtcacgcag gaacaggcac
     7201 tgggcctggg ccacgcggtg ctgtgcgcca agccggtcat cggcgacgag ccgttcgggg
     7261 tgatcctgcc cgacgacctg atcgtcaacg gcggcgccgg agccctgcgc cagatggccg
     7321 aacgcgcggc cgacggcagg accggcgtgc ttgcggtcga ggaagtaccc cattccgata
     7381 ccggcaaata cggcatcgtc gacgtcggtt ccgatggtga tcgcatccgc cacatggtcg
     7441 aaaaaccgaa gcccgaggac gctccgtcga acctcggggt cgtcggccgc tacgtgctgc
     7501 cggcgcgcat
//
LOCUS       DASSNH010000248         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1437826, whole genome shotgun sequence.
ACCESSION   DASSNH010000248 DASSNH010000000
VERSION     DASSNH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437826"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="VFK00_09170"
     CDS             <1..1487
                     /locus_tag="VFK00_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7593160.1"
                     /translation="TANIWEYGKSGLYDVDSATGHAVLIGEIDHGGMSLLAHDGVARI
                     AAGSNTQMHAAAFHSTNGRTWKPLPESVVGKSFDPLTISADGKHLYALSSLDGGPDQL
                     IECDLDGGNRKVLASDSFSSVTDVMWNPETRTPVAAVFAAGGKPKVQYISNGIYAQVM
                     QALARAQPDAFVSMEGASADGSALLISVRSDRNPGALALFDKAHMHARPLYQVLPWID
                     PARMPVRMPIRFTDRAGVELAGFLTLPSGGDHKNLPLVLIPHGGPIGPSDGWFYDPWA
                     ALLADRGYASLQINYRGSGERGFNFGRSGYRQFATGIQEDLIDGVKWAIAQGYVDPNR
                     ICVFGGSFGGYSSLMQPILAPGLYKCAVDYAGVYDWRIGMDRSDTRRLAGGQVYFKDA
                     IGTREDAYAMSPVSMMDKFNVPVLIAHGKDDPRVPYQNATDLRSYLDKAGKPYEWLAQ
                     PGELHGFASEEHNEEFFGMITAFLAKHIGPGDAAPAKSAGAAPE"
     gene            1564..>2445
                     /locus_tag="VFK00_09175"
     CDS             1564..>2445
                     /locus_tag="VFK00_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HET7593161.1"
                     /translation="MPNRLASASSPYLRMHAGNPVDWQPWDEAALAQARDADTPVLLS
                     VGYSACHWCHVMARECFEDPEIAAAMNASFVNIKLDREERPDIDRVYQLAHQALAGRG
                     GGWPLTAFLDPQDLSPFYIGTYFPPSPRHGLPGFPELLQRVRAYFDSHRTELQANAEQ
                     LRAWMRQVQAPAPGPVPDAESAAHMGLQRIAARADPDWGGTRGAPKFPRAIELEWLLD
                     PAPFGRSSRAAPDSPEGKAVRKQAHLTLATMAARGLQDHLGGGFFRYSVDASWTIPHF
                     EKMLYDNAQLLPVYARLA"
BASE COUNT          410 a          841 c          804 g          390 t
ORIGIN      
        1 ccaccgccaa catctgggag tatggcaaga gcgggctcta cgatgtcgac agcgccaccg
       61 gccatgccgt tctgatcggc gagatagacc atggcgggat gtcgttgctt gcgcatgacg
      121 gggtcgcgcg catcgccgcg ggctcgaaca cccagatgca cgccgcggcc ttccacagca
      181 ccaacggcag gacctggaag ccgctgcccg aatcggtggt gggcaagtcg ttcgacccgc
      241 tcacgatcag cgccgacggc aagcacctgt acgcgctgtc cagcctcgat ggtggtccgg
      301 accagttgat cgaatgcgac ctcgatggcg gcaaccgcaa ggtgctggct tcggattcgt
      361 tctcctcggt caccgacgtg atgtggaacc ccgagacccg tacgccagtc gccgccgtct
      421 tcgccgcagg cggcaagccg aaggtgcaat acatctccaa cgggatctac gcgcaggtga
      481 tgcaggcgct ggcacgcgcg cagccggacg cgttcgtgtc gatggagggc gccagcgccg
      541 acggctcggc cttgctcatc agcgtgcgca gcgaccgcaa tcccggcgcc ctggccttgt
      601 tcgacaaggc ccacatgcac gcccgcccgc tctaccaggt gctgccgtgg atcgatcccg
      661 ccaggatgcc ggtccggatg ccgatccgct ttacggaccg tgcaggggtc gagctggcgg
      721 gattcctgac gttgcccagc ggcggcgacc acaagaacct gccgctggtg ctgatccccc
      781 acggcggccc gatcggccca tccgatggct ggttctacga tccatgggcc gcgctgctgg
      841 ccgaccgcgg ctacgcgagc ctgcagatca attaccgcgg ctcgggcgaa cgcggcttca
      901 acttcggccg ctccggctac aggcaatttg ccacgggcat ccaggaggac ctgatcgacg
      961 gcgtgaaatg ggcgatcgcc cagggctacg tggacccgaa ccgcatctgc gtgttcggcg
     1021 gaagcttcgg cggctattcc tcgctgatgc agcccatcct cgcgcccggg ctctacaagt
     1081 gcgcggtcga ttacgccggc gtgtacgact ggcgcatcgg catggacagg agcgacacgc
     1141 ggaggcttgc cggcggccag gtgtatttca aggatgccat cggcacccgc gaggacgcct
     1201 acgcgatgtc gccggtcagc atgatggaca agttcaacgt gccggtgctg atcgcgcacg
     1261 gcaaggacga cccgcgcgtg ccgtaccaga acgccacgga tttgcgctcg tacctggaca
     1321 aggcgggcaa gccctacgaa tggctggcgc aaccggggga actgcacggc ttcgcgagcg
     1381 aagaacacaa cgaggaattc ttcggcatga tcaccgcctt cctggccaag cacatcgggc
     1441 cgggcgacgc ggcgccggcg aagtccgcag gggccgcacc ggaatagcgg caacgctgcg
     1501 atcctgattc gaagcccggc accggccggg cttctttttt ggggccgcgc tagcatggca
     1561 gccatgccca atcgcctcgc cagcgcgtcg agcccgtacc tgcgcatgca tgccggcaac
     1621 ccggtggact ggcagccgtg ggacgaggcg gcgctcgcgc aggcgcgcga cgccgacacg
     1681 ccggtcctcc tttcggtcgg ctacagcgcc tgccactggt gccacgtgat ggcgcgcgag
     1741 tgcttcgagg atcccgagat cgcagcggcg atgaacgctt cgttcgtcaa catcaagctc
     1801 gaccgcgagg agcgcccgga tatcgatcgc gtgtaccagc tcgcgcacca ggcgttggca
     1861 ggtcgcggcg gcggctggcc gctgaccgcg ttcctggatc cgcaggattt gtcgccgttc
     1921 tacatcggca cctatttccc gccgtcgccg cggcatggat tgcccgggtt tcccgaactc
     1981 ctgcaaaggg tgcgtgccta cttcgactcg caccgcaccg agctgcaagc gaacgccgag
     2041 caactgcgcg cctggatgcg ccaggtgcaa gcccccgcgc cgggcccggt tcctgacgcg
     2101 gagtcggccg cccacatggg gttgcaacgc atcgctgcgc gcgccgatcc cgactggggc
     2161 ggtacccgcg gcgcgcccaa gttcccgcga gccatcgaac tggagtggct tttggaccct
     2221 gctcccttcg ggagaagctc gcgcgcagcg ccggactccc ccgaagggaa agcggttcgg
     2281 aagcaggcgc acctcaccct cgcgaccatg gccgcccgcg gcctgcagga ccatctcggc
     2341 ggcggttttt tccgttactc ggtggacgcg agctggacca tcccgcactt cgaaaaaatg
     2401 ctctacgaca acgcgcagtt gctgccggtg tatgcgcggc tcgcg
//
LOCUS       DASSNH010000249        16698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_157095, whole genome shotgun sequence.
ACCESSION   DASSNH010000249 DASSNH010000000
VERSION     DASSNH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 16698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16698
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157095"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFK00_09180"
     CDS             complement(<1..343)
                     /locus_tag="VFK00_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593162.1"
                     /translation="MSIRSGLLAGSLVLALGCSSLAGAESTTQAMPETMDMLRHAIAA
                     QTVEGKDQVPAFARYLAGKLESAGFAESDIEIIPVEHTAALVVHYRGSDKSLKPILLS
                     AHMDVVAANPRD"
     gene            complement(414..1055)
                     /locus_tag="VFK00_09185"
     CDS             complement(414..1055)
                     /locus_tag="VFK00_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7593163.1"
                     /translation="MRTRGSSNRRTLRSLCLALPLLALATATVAQPVTYTIDPDHTYP
                     SFAADHMGLSTWRGKFDHSKGTIMLDRTKHTGTIHIVTQIDSINFGNPPLKQMVLRDA
                     MPASLCKTQCAFFGAAKYPTATYDGTLADFVNGAPTRVVGKLTLRGVTRPLDLEIEHF
                     KCIPDFVLKPRERCGAEASATFDRSEFGIDAGKSFGLDMKVGLQIQVEAVQDK"
     gene            complement(1061..1483)
                     /locus_tag="VFK00_09190"
     CDS             complement(1061..1483)
                     /locus_tag="VFK00_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7593164.1"
                     /translation="MQLANYLFFDNQAGAAFDFYARCLGGEVAMKVTFGDMPGPSDLP
                     PEAEDLIAHVRLQVGDAVLMGSDWCTPPDGEAYPGIHGNAVSVSVDKPEEAERLFEAL
                     SEGGKVTMPITETSWALRFGMFTDRFGAKWMINCNRPE"
     gene            complement(1572..2198)
                     /locus_tag="VFK00_09195"
     CDS             complement(1572..2198)
                     /locus_tag="VFK00_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7593165.1"
                     /translation="MMQLYWADVLSPRKACAVAKYLGSPIEYVYLDLGRGEQRTPGYL
                     ALNPNGKVPTLVDGDRVLWEADAIMCHLAGRCDSDLWPQDARQIDVLRWLSWNLQHFS
                     RHAGSLYFEYVIKPRFGLGEVDPATIAQATDDWRRFARVLDAHLRDRDWLVGTALTVA
                     DFAVAVALPDAERAHIPVDEFPAICRWHDRLNGIEAWRVPFPAPALAA"
     gene            complement(2195..2665)
                     /locus_tag="VFK00_09200"
     CDS             complement(2195..2665)
                     /locus_tag="VFK00_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET7593166.1"
                     /translation="MEPDAALSETTGCLCLASRRAARAITREFDSALRSRGLRATQFT
                     LLAALHLTGPRSIGELAELLSADRTTLTRNLAVAEQHGWVVTRPCRDDARARIAAIAP
                     KGSRVLKAALPAWRKVQQRLLGEIGPRAAESLRRLAGGACALPAPHLQTGREQP"
     gene            complement(2829..4493)
                     /locus_tag="VFK00_09205"
     CDS             complement(2829..4493)
                     /locus_tag="VFK00_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7593167.1"
                     /translation="MTNEYAVRRTVGAWALMFTGLGSIIGSGWLFGAWRAAQLAGPGA
                     IWAWVIGAVVILFIAVTYAELGAMFPESGGMVRYGQYSHGTLVGFIAAWANWIAIASV
                     IPVEAEASVQYMASWPWQWAQDLYHVAPGGLGELSPTGLGISAALVIVYFLLNYWSVN
                     LFAKTNTWITIYKLVVPALTAIALMATSFHPENFSVGAHGGAHAISLSAVLTAVAISG
                     IVFSFNGFQSPVNLAGEAKNPGRSVPFGVIGSIVLATVVYLLLQVAFLGATEPALLAK
                     GGWAAIEHSSPFAQLAIALNLNWLALLLYSDAFISPSGTGATYTATTARMIYGMQRNG
                     TLPKVFGKVHPRFGIPRPAMWLNLVVAFIFLFFFRGWAALAEVISVATIISYLTGPVS
                     VSVLRRTAPELHRPLRIPLLPVIAGLAFVFATELLYWARWPRTGEIILLMVVALPVWF
                     WYAVRRGGGDLLLQVRGALWLIFYLPAVAALSWAGSEKFGGHGYLSYGWDLAIVAAVA
                     IGFFVWGLACGWRTPEVEAIKMEAHAHPDAIMVPPDEETAERMAGR"
     gene            4982..6865
                     /locus_tag="VFK00_09210"
     CDS             4982..6865
                     /locus_tag="VFK00_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7593168.1"
                     /translation="MWRVAIAYAVVAWLLLQLLSIVFPTFGAPAWVLKVVTVLLAAGL
                     PVALVLAWAFEVTPEGVRRTEPVGSPQSRPEDQSHRIGRTLNTAIIVALILAVGLLGW
                     RLMVLRHAPAAPSLVASATRSPDAAKRNPGISSKAAPGSAAGAAASGLQADAVPVSAA
                     SIPEKSVAVLPFVNESGAKDQRFFSDGLSEDLITALSQFAGLKVISRNSAFQFRDSKD
                     SSSEIGKLLGVAHLLEGSVQRAGDEVRITATLVNAADGSVLWTQRYDKPYKDLFALQD
                     AITTAVASALKAKLLTAPGAVVQSDRPPSGNLAAYTAFLRGIALNASATEVDERKAID
                     AFALAIKLDPKYARAYAAQGRAWAGLGAIFLGGAAHERAYDKARNAIGRALHVAPDLA
                     AGYAARSYLEAYGEFDWQSALADAQRAVQLAPDDSDAQLQLGGILAALGQVDRAVNLT
                     RQALKADPRNGGAYFNLSWFFASLGQFENAEQAIRTAIEVQPQGAANHEQLAVVAILS
                     GDTSVALTAAHKETSLPWRESALALALQNSPDRAAADAALKTLTAAQGSAYQVAEVYA
                     LRHDPDNMFQWLDRAWNNRDGGLGYLLSDPFILRYRYDPRFAAFCEKVGLPTTTDAVA
                     MKL"
     gene            complement(7036..8370)
                     /gene="pepQ"
                     /locus_tag="VFK00_09215"
     CDS             complement(7036..8370)
                     /gene="pepQ"
                     /locus_tag="VFK00_09215"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HET7593169.1"
                     /translation="MTASPQDNLSALYPAHLAEIEKRSADALAAAGRDTLAIAAGRPH
                     GWFLDDQDYPFKANPHFLHWLPLTGAPGSWIIYTPGSKPKLVFLQPDDYWHVVPAAPS
                     GYWVEHFDIVVIRKPEDARAHLPKDPSRCAVIGEANAAVGDIEPDNPEAALNLLHYRR
                     AYKTPYELTMMRVASKLGVHGHRAAERGFREGLSEFDIHCRYLEATRLTESQLPYGNI
                     IALDQHAAVLHYMHFDRNPPTHAHSFLIDAGGQFHGYASDITRTHASPDAGEFQALID
                     SVERAQHGFCAMVRAGRSYPELHLQAHHVLMQAMHDHGFITCSPEAAVASGISATFFP
                     HGLGHSIGLQVHDVAGFARDEQGGMIAKPQGHPNLRLTRTLAPDMVVTIEPGLYFIDM
                     LLAELKSRPAAKDVAWSNVDAFRKYGGIRIEDDVVCTHGEPVNLTREAFAAA"
     gene            complement(8592..8933)
                     /locus_tag="VFK00_09220"
     CDS             complement(8592..8933)
                     /locus_tag="VFK00_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7593170.1"
                     /translation="MNDERRSAPRKPSMAPIEVIDTVSGEPIGRLGNLSRNGMMLICH
                     RPLHDNALYQLRFRLPDRRGGSPCEIETGVHTMWSENASTNGYQWAGLRIISISGPSA
                     ASLDRWLDAAA"
     gene            complement(8930..11128)
                     /locus_tag="VFK00_09225"
     CDS             complement(8930..11128)
                     /locus_tag="VFK00_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HET7593171.1"
                     /translation="MEKHPTLADRSDLAPRARHLLAQQLAECGGLLEPLLRNVLDDLE
                     HELFRIGEQSSGGQVQHEAMEALREVKQSRPLFQGRLMAALERRFAGIGRPAPAAARA
                     AQPLASEGLSLVDPHEFDESMALDDLSTRTELHAGSAIFELCYRYGVLLASPPLESEV
                     LPAGPQVLAAVLGEAANGLAIEREQRLLFYRLCDRRLFGAAESFYGRLNEHLKACGIL
                     PDLRSYLPRRGASEQRSADAPSPLEAPETRSIPPAVNFTPPPAAGDLHNLLDARRRTL
                     HPEDAAAAQSGEFASMPQLQDALAALQALPPRRVGPGAGENLAKELAAELQRGASGGK
                     RPQLQPEHRDALDLIGLLYDQLLTETRAEGVAQQLLSSLQVPLLRIALSDSNFFTVRE
                     HPARRLLNLVLETANLWLDRSSDSFDAALNQRLQRSVQRITSDFQGDIGIIEREADDL
                     DSHLRLLSRRAEIAERRQVEAAQGRDRLREARTTAGAAISKRLAGRNTTPLVRALLES
                     AWTDALTLTLLREGENSEAYRRRLADADQLLDSPVGRDDTRLAADLNHGLTQVGLQAS
                     EADQITRYALDLPQRPSSRAATPPPSQTELLIRLKSRHKRGEGEAAPAAPAAPQKLPL
                     TPSERRALDELKRLPFGSWLEFTINQQGQKVARKLAWHNPVSGRCLLVNARGAAAPER
                     TLEQIARLMARGQVRLLPPQDKGMIDRAWDALVTGLRKFSGTNLTTETAT"
     gene            complement(11223..11405)
                     /locus_tag="VFK00_09230"
     CDS             complement(11223..11405)
                     /locus_tag="VFK00_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HET7593172.1"
                     /translation="MPDFVPVGCPYCGESVELIVDSSAGSAAYIEDCPVCCRPMQVTV
                     EVDGEAVTVRVRTGDE"
     gene            complement(11398..12696)
                     /gene="hemW"
                     /locus_tag="VFK00_09235"
     CDS             complement(11398..12696)
                     /gene="hemW"
                     /locus_tag="VFK00_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7593173.1"
                     /translation="MSACLERPPSTADSLSGRSAGSPLAPGPRSPVPLSLYIHIPWCV
                     RKCPYCDFNSHGLRSELPVREYVAALMADLDGDLADFGTALAGREIASVFFGGGTPSL
                     MPPEAIAAIFDGAAARLTFAHDCEITLETNPGTVEHGRFDGYLAAGVNRISFGVQSFD
                     DEKLGALGRIHSAAEAETAVKLAQDAGYDNINLDLMYALPEQRPEGALADVERALALQ
                     PAHISHYQLTLEPGTPFAKRPPSLPDDDAAWDMQEACHARLAAAGFAQYEVSAYARNG
                     RRCRHNLNYWEFGDYLAIGAGAHGKISGPGTGDPGPGKTGEFAVQRRWKVRSPRAYLQ
                     KAATGQRVGGDEAVAAEQLPFEFMLNALRLGDGIAAQAFTVRTGLPLSAIDAPLGNAR
                     DRGWLVHDPAFLRTTPLGRRFLNDVIASFLPEPARRYRHA"
     gene            complement(12697..13323)
                     /gene="rdgB"
                     /locus_tag="VFK00_09240"
     CDS             complement(12697..13323)
                     /gene="rdgB"
                     /locus_tag="VFK00_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET7593174.1"
                     /translation="MGNRIVLASGNTGKLAELRELLAGSGIEFIPQTELGIGGAEETG
                     HTFVENAILKARHAARASGLPALADDSGICVDALGGAPGLDSALYAGVHGADEANNDK
                     LLRALADVPDEARTAHYTCVVVLLRSAADPDPLIATGRWRGRILRRRRGARGFGYDPL
                     FLPEDGGGLSSGELDPAEKNRISHRGQALAALREILGTRNREPGTGKR"
     gene            complement(13344..14078)
                     /gene="rph"
                     /locus_tag="VFK00_09245"
     CDS             complement(13344..14078)
                     /gene="rph"
                     /locus_tag="VFK00_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7593175.1"
                     /translation="MATTRPSGRAPDELRAVTIERHYTRHAEGSVLISAGDTRVLCTA
                     SVEERIPPWLRGKGQGWVTAEYGMLPRATSDRTQREAARGGQGGRTMEIQRLIGRSLR
                     ACVDRVALGERVVTLDCDVLQADGGTRTAAITGAYVALVDAVDVLMRRGLLKRNPIHG
                     AVAAVSVGIFQGVPVLDLDYAEDAHCDTDMNIVMNDGGGFIELQGTAEGHAFRREELD
                     TLLSLAEKGVGELIAKQRAALARRES"
     gene            14146..15006
                     /locus_tag="VFK00_09250"
     CDS             14146..15006
                     /locus_tag="VFK00_09250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7593176.1"
                     /translation="MIRSMTAYASDEETTPLGWLACEVRAVNHRFLETGVRLPDELRA
                     LEPQVREHIAARLSRGKLDATLRYRAPSAAADLLLDERTAMQLSTAAETLKARFPQLA
                     TDFAALLDWPGLLVKPELDQEGVAQAALALLDRTLDEFIAGREREGEKLAAALRDRLD
                     GIEHILAGLRAILPDIRKALRAKLQARLGELQAAADPGRLEQELVLQLSRIDVDEELD
                     RLDAHVGEARRVLTLSEPVGRRLDFLMQEFNREANTLASKSADQRTTRAAVDLKVLIE
                     QMREQVQNLE"
     gene            15415..>16698
                     /locus_tag="VFK00_09255"
     CDS             15415..>16698
                     /locus_tag="VFK00_09255"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HET7593177.1"
                     /translation="MAGCRGLVRSLLWVSLAVAGVAHAGNAPTGGQIAAGSGQIQQSG
                     NTTTIRQDSQTLSLNWQSFDIGAAQTVQFLQPGADSIAVNRILGSTASEIQGHLEANG
                     QVWLINPNGVLFGRDAQVNVGGLVASTLAVDDSTIGSGDVRFAGNGKGKVVNRGRITA
                     ADGGYVALLGNQVSNQGVIRARLGTVALGGGTAVTLTFADHHLMHLQVDANAVRSLVE
                     NRQLIVADGGAVLMTAGARDSLVASAVNNSGTVQARSVEEHDGTVTLLGGMQAGSVQV
                     GGTLDASAPDGGDGGQIETSAAHFELADDARITAAAAGGKAGTWLVDPVDLTIDAAAA
                     STISGTLEGGTNVIEQTTATGATGTGTKTSGNGDINVDAALSWTNPSATLTLDAYHDI
                     NVNAPINGSGGIVMKAANGSVTIGSGGSLQAGSGAT"
BASE COUNT         2731 a         5500 c         5745 g         2722 t
ORIGIN      
        1 agtccctggg attggcggcg accacgtcca tgtgcgcgga cagcaggatc ggcttcaggc
       61 tcttgtcgct gccgcggtag tgcaccacca gcgcggcggt gtgttcgacc ggaatgatct
      121 cgatgtcgga ctcggcgaag ccggccgatt ccagtttgcc ggccaggtac cgcgcgaaag
      181 ccggcacctg gtccttgcct tcgaccgttt gcgcggcgat cgcgtgccgc agcatgtcca
      241 tggtctcggg catcgcctgc gtggtgcttt ccgcgccggc gagcgaagaa cagccgagcg
      301 ccaggaccaa actgcctgcc aacagcccac tgcggatgga catgaagttt cccgttcagt
      361 cgaagtggct cagggtaccg caaacgggtt ccgcggcatg gcggcgatgc ggactatttg
      421 tcctgcaccg cctccacctg gatctgcagg cccaccttca tgtcgaggcc gaacgacttt
      481 ccggcgtcga tcccgaattc ggagcggtcg aacgtggcgg atgcttccgc gccgcagcgt
      541 tcgcgcggct tgagcacgaa atcggggatg cacttgaagt gttcgatctc caggtccagc
      601 gggcgggtca cgccacgcag cgtgagtttg ccgaccacgc gcgtgggcgc accgttcacg
      661 aaatccgcaa gcgtgccatc gtaggtcgca gtcggatact tcgcggcgcc gaagaatgcg
      721 cattgcgtct tgcacagcga cgccggcatg gcatcgcgca gcaccatctg cttcagcggc
      781 gggttgccga agttgatgct gtcgatctgc gtgacgatgt ggatcgtgcc cgtgtgcttc
      841 gtccgatcca gcatgatcgt gcccttgctg tggtcgaact tgccgcgcca ggtcgagagg
      901 cccatgtggt cggcggcgaa gctcgggtag gtgtgatccg ggtcgatggt gtaggtgacc
      961 ggctgggcca ccgtcgcggt ggccagcgcc agcagcggca gcgcgaggca gagcgatcga
     1021 agggtgcgtc gattggatga gccgcgtgtg cgcatggtgt tcattccggc cggttgcagt
     1081 tgatcatcca cttcgcgccg aagcggtcgg tgaacatccc gaagcgcagg gcccaagagg
     1141 tctccgtgat gggcatcgtc accttgccgc cttcgctgag cgcctcgaac aggcgttccg
     1201 cctcctcggg tttgtcgacg ctcaccgaca ccgcgttgcc gtggattccg gggtacgcct
     1261 cgccgtcggg cggcgtgcac cagtccgaac ccatcaggac ggcatcgccg acctgcagcc
     1321 ggacgtgcgc gatcagatcc tcggcttccg gcggaaggtc cgaaggtccc ggcatgtcgc
     1381 cgaaggtcac cttcatcgcg acttcgccac cgaggcatct cgcgtagaaa tcgaaagccg
     1441 ctccggcctg attatcgaag aacaggtagt tcgcgagttg catgacactc ctccgaatga
     1501 tggtggacga ctcggccctt gcgggactcg ataccgcgac gaacgaccaa gccggagatc
     1561 gacatcgacg ctcaggccgc caaggcaggc gccgggaagg gtacgcgcca ggcctcgatc
     1621 ccgttcaggc ggtcgtgcca gcggcagatc gcgggaaatt catccacggg aatgtgcgcg
     1681 cgctcggcgt ccggcagcgc caccgcgacc gcgaaatcgg ccacggtgag cgcggtgccg
     1741 accagccagt cgcggtcgcg cagatgggca tcgaggaccc gtgcgaagcg gcgccaatcg
     1801 tcggtggctt gcgcgatcgt ggccggatcg acctcgccca atccgaaacg cggcttgatg
     1861 acgtactcga agtacagcga cccggcgtgc cgcgagaaat gctgcaggtt ccaactcagc
     1921 cagcgaagca cgtcgatctg gcgcgcgtcc tgcggccaca ggtcggagtc gcagcgcccc
     1981 gcgaggtggc acatgatcgc gtcggcttcc cacaggaccc ggtcgccgtc gaccagcgtc
     2041 ggtaccttgc cgttgggatt cagcgcgaga tatccaggcg tgcgctgctc gccacggcca
     2101 aggtcgagat acacgtactc gatcggggag cccaggtatt tcgcgaccgc acaggccttg
     2161 cgcggcgaca gtacgtcggc ccagtaaagc tgcatcatgg ctgttccctt ccggtctgga
     2221 ggtgaggcgc cgggagcgca caagcgccgc ctgccagccg gcgcaggctt tcggcggcgc
     2281 gtggaccgat ttcgccgagc aggcgttgct ggaccttgcg ccacgcaggc agcgcggcct
     2341 tcagcacgcg cgaacccttc ggcgcgattg cggcgatgcg tgcgcgggca tcgtcgcggc
     2401 agggccgcgt caccacccag ccatgctgct ccgcaacggc caggttgcgg gtgagggtgg
     2461 tccggtcggc ggacaacagt tcggcgagtt cgccgatcga tcgcgggccc gtgaggtgca
     2521 gcgccgcgag caacgtgaac tgggtggcgc gtaggccccg cgaccgcagc gcgctatcga
     2581 attcgcgcgt gatcgcccgc gccgcacgcc gcgaggccag gcacaggcac ccggtggtct
     2641 cggacagggc ggcatcgggc tccatgtaat gagtgtatgt acacgcaatc gatgtggtca
     2701 aggccgcagc ggcggcccgc cgaaagccgt gaagccgcct gcggtgcccg gccggccatg
     2761 gcgccgtacg acgcctgcgc aagcatggcc gggcgacttt ttttggccct ctccaaacgc
     2821 cgcagcgctt accggcccgc catgcgttcc gcggtctctt catccggcgg caccatgatg
     2881 gcatccgggt gcgcgtgggc ctccatcttg atcgcctcga cttccggagt gcgccagccg
     2941 caggccagtc cccagacgaa gaagccgatc gcaacggcag cgacgatcgc gaggtcccat
     3001 ccgtacgaaa gataaccgtg gccgccgaac ttctcactgc cggcccacga cagcgccgcg
     3061 accgcgggca ggtagaagat cagccacagc gcgccgcgca cctgcagcag caggtccccg
     3121 ccgccgcgcc tgaccgcgta ccagaaccac accggcagcg cgaccaccat cagcaggatg
     3181 atctcgccgg tgcggggcca gcgcgcccag tacaggagtt cggtcgcgaa cacgaaggcg
     3241 agtcccgcga tcaccggcag cagcgggatc cgcagcggcc ggtgcaactc gggcgcggtg
     3301 cggcgcaaca ccgatacgct caccgggccg gtcaggtacg aaatgatcgt cgcgaccgaa
     3361 attacttcgg ccagcgccgc ccagccccgg aagaagaaca ggaagatgaa cgcgacgacc
     3421 aggttcagcc acatcgccgg gcgcgggatg ccgaagcgcg gatgcacctt gccgaacacc
     3481 ttgggcagcg tgccgttgcg ttgcatgccg tagatcatcc gggcggtggt cgcggtgtag
     3541 gtcgcgccgg tgccggacgg gctgatgaag gcgtcggaat acaacagtag cgccagccag
     3601 ttgaggttca acgcaatcgc caattgagcg aaaggcgagg aatgttcgat cgcggcccag
     3661 ccgcctttcg cgagcaacgc gggttcggtc gcgccgagga aggcaacctg cagcaacaga
     3721 tagacgacag tcgccagcac gatcgatccg atcaccccga acggcacact gcgccccggg
     3781 ttcttcgctt cgcccgcgag gttcacgggg ctctggaaac cgttgaagct gaacacgatg
     3841 ccgctgatcg cgaccgccgt gaggaccgcc gacaagctga tcgcatgggc gccgccgtgc
     3901 gcgcctacgc tgaagttctc cggatggaac gaagtcgcca tcagcgcgat cgcggtcagc
     3961 gccggcacca ccagcttgta gatcgtgatc caggtgttgg tcttggcgaa gaggttcacg
     4021 ctccagtagt tcaggaggaa gtacacgatc accagcgccg ccgagatgcc gaggccggtc
     4081 gggctcagct cgccgagacc gcccggcgcg acgtgataca gatcctgcgc ccactgccac
     4141 ggccaggacg ccatgtactg caccgaagcc tcggcctcga ccgggatcac cgacgcgatc
     4201 gcgatccagt tggcccaggc cgcgatgaag cccaccagcg tcccgtgcga atactggccg
     4261 tagcgcacca tgccgcccga ttccgggaac atcgccccga gctcggcgta ggtgaccgcg
     4321 atgaacagga tcaccaccgc gccgatcacc cacgcccaga tcgcgcccgg gcccgcgagt
     4381 tgcgccgcgc gccacgcgcc gaacagccag cccgatccga tgatcgagcc caggcccgtg
     4441 aacatcagcg cccatgcgcc cacggtgcgg cgaactgcgt attcattggt catcgatgga
     4501 ttccacccgg aaaatgtgcc ggatgatcgc agatcacggc gtgtctgtcc gcgtggcgat
     4561 cgtcatgggt gccagccatt cgctgtcgct gaaccccggc agatggcgcg agtcaaccgg
     4621 ctttgggtgt ggactcggga gcagatcccg gatcgccgcc gcgcggcgtc cggagtgacc
     4681 atggggctgc gccacgacca tcgacaccgt gaaacaacac cccgtgagct tgcgaagggc
     4741 gttgatgcaa agcatcgggg ggatggcttc tgtcacgcga ggcacgccgg taaccaggtt
     4801 gcaacaaccg catccctcca tctctccatt gcgacagcta cgttgcgagc ccgatcgcac
     4861 ctgaagctgc cgacgcaatg atttacactc gcgtgcggag gccggggcgt gcccggcggg
     4921 ggatccgcgt gaggtggcag tccacaagcc cggtttcttc gaggaactga aacgccgcca
     4981 cgtgtggcgc gtcgcgatcg cgtacgccgt ggtcgcatgg ctgttgctgc aactgctttc
     5041 catcgtcttt ccgaccttcg gggcgccggc gtgggtgctg aaggtggtga cggtgttgct
     5101 ggccgcgggg cttcccgtcg cgctggtgct ggcgtgggcc ttcgaggtca cgccggaggg
     5161 cgtgcgccgc accgagccgg tcggttcgcc ccagtcccgc ccggaggacc agtcccaccg
     5221 catcggccgc accctcaaca cggcgatcat cgtggcgctg atcctggccg tggggctgct
     5281 cggctggcgg ttgatggttc tgcggcatgc gcctgccgca ccatcactgg ttgcaagtgc
     5341 aacccgtagc ccggatgcag cgaagcgcaa tccgggaata tcgtccaagg ccgccccggg
     5401 ttccgccgct ggcgcggctg catccgggct gcaggccgat gccgtcccag tatctgcggc
     5461 cagcatcccc gaaaaatccg ttgcggtatt gccgttcgta aacgagagcg gcgcgaagga
     5521 tcagcggttc ttttccgacg gtctttccga ggacttgatc accgcgctat cccagttcgc
     5581 gggcttgaag gtcatcagcc gcaattcggc gttccagttc cgcgacagca aggattcgag
     5641 ctccgaaatc ggcaagcttc tcggcgtggc gcacctgctg gagggcagtg tgcagcgcgc
     5701 gggcgacgaa gtgcgcatca ccgcgacgct ggtcaacgcg gccgatggct cggtgttgtg
     5761 gacgcagcgc tacgacaagc cgtacaagga cctgttcgcg ctgcaggacg cgatcacgac
     5821 agccgtggcc agcgcgctaa aggcgaagct gttgaccgcg cccggtgcgg tggtgcagag
     5881 cgaccggcca cccagcggga atctcgccgc ctacaccgct ttcctgcgcg ggattgccct
     5941 caatgccagc gcgaccgagg tggatgagcg taaggccatc gatgcattcg cgctggccat
     6001 caagcttgat ccgaaatatg cgcgggcgta cgcagcccaa gggcgagcgt gggcgggtct
     6061 gggtgcgatc tttctgggtg gagccgccca tgaacgggcc tacgacaagg cgcggaatgc
     6121 catcggcagg gctctgcatg tggccccgga ccttgcagcg ggttacgccg cgcgcagtta
     6181 tctcgaggca tacggcgaat tcgactggca atcggcactc gccgacgcgc agcgggccgt
     6241 gcaactggcg cccgacgaca gcgatgcgca attacagctg ggcggcattc tggcggccct
     6301 gggacaagtt gaccgcgcag tgaatttgac taggcaggcg ctgaaggccg accctcgcaa
     6361 tggcggtgcg tacttcaatc tgtcatggtt cttcgcaagc ttggggcagt tcgaaaatgc
     6421 cgagcaggcc atccggacag cgatcgaggt tcagccgcag ggagcagcca atcacgaaca
     6481 gttggcggta gtcgcaatcc tgagcggcga tacgagcgtt gcgctgacag cggcgcacaa
     6541 ggaaaccagc ctgccctggc gtgagtccgc gctggcgctg gctctgcaaa acagtcccga
     6601 ccgagcggcc gccgatgcgg cactgaagac attgaccgcg gcgcagggga gtgcctacca
     6661 ggtagcggag gtgtatgcgc tgcgccacga cccggacaat atgttccagt ggctggatcg
     6721 tgcgtggaac aaccgagacg ggggtctggg ttatttgctc tccgacccct tcatcctgcg
     6781 ctatcggtac gacccgcgct tcgccgcgtt ctgcgagaag gtcggcctgc ccactaccac
     6841 cgacgccgtt gcaatgaagt tgtaagagcg gcccgtggcc gcggccggtc cagccgatcg
     6901 caatccgaga cacgcccagc tttcgtaggt tgggctgatc ccgggcttta cccgcgagaa
     6961 gcccaacatt gccggcacgc acgcatcgtg atgttgggct tcgctgcgct cagcgccaac
     7021 ctgcgaactt cgcatctacg ccgcggcaaa tgcttcgcgg gtgaggttga ccggctcgcc
     7081 gtgggtgcag accacgtcgt cctcgatgcg gatgccgccg tacttgcgga atgcatcgac
     7141 gttcgaccac gcgacgtcct tggcggcggg cctggacttc aattccgcga gcagcatgtc
     7201 gatgaaataa agcccgggct cgatggtgac gaccatgtcg ggcgcgagcg tgcgggtgag
     7261 gcgaagattg ggatggcctt gcggcttcgc gatcatgccg ccttgctcgt cgcgcgcgaa
     7321 gccggcgacg tcgtgcacct gcaggccgat cgaatggccg aggccgtgcg ggaagaacgt
     7381 cgcggaaatg ccgctggcca ccgccgcttc gggcgaacag gtgatgaagc cgtggtcgtg
     7441 catcgcttgc atcagcacat ggtgcgcttg caggtgcagc tccggatagc ttcgccccgc
     7501 acgcaccatg gcgcagaagc catgctgcgc ccgctcgacc gaatcgatca gggcctggaa
     7561 ctcgcccgca tccggcgatg catgcgtgcg cgtgatgtcg gaggcatagc cgtggaactg
     7621 cccgccggca tcgatcagga acgaatgcgc atgcgtaggc gggttgcggt cgaagtgcat
     7681 gtagtgcagc acggcggcgt gctggtccag cgcgatgatg ttgccgtagg gcaactgcga
     7741 ttcggtcagc cgggtggctt cgagataacg gcagtggatg tcgaattccg acaggccctc
     7801 gcggaacccg cgctcggcgg cgcggtgccc gtgcaccccg agcttgctcg cgacccgcat
     7861 catcgtcagt tcgtacggcg tcttgtacgc gcgccggtag tgcagcaggt tcagtgcagc
     7921 ctccggattg tccggctcga tgtcgccgac cgcggcattg gcttcgccga tgacggcgca
     7981 gcgcgatggg tccttcggca ggtgcgcgcg cgcgtcttcg ggtttccgaa taaccacgat
     8041 gtcgaagtgc tcgacccaat atccggaagg cgccgccggc acgacgtgcc agtaatcgtc
     8101 cggttgcagg aacaccagtt tcggcttgct gccgggcgta tagatgatcc agcttccggg
     8161 cgcgccggta agcggcagcc agtgcaggaa atgcgggttg gccttgaagg ggtaatcctg
     8221 gtcgtcgagg aaccagccgt gcggccggcc ggcggcgatg gccagcgtgt cgcgcccggc
     8281 cgcggcgagg gcgtcggcgc tgcgcttttc gatctctgcg aggtgcgccg ggtacagcgc
     8341 cgaaagattg tcctgcgggg aagcggtcat ggcgtgcggc gtcgaaaagg agctgccatg
     8401 atcccgcgac gcgcgccgca aagcaaaaca cccgcgcggc acgtcttgta cgggtgccaa
     8461 gttgaccagg atttccttcg gagcggctgt tgtggaaagt caggccgtgg atggccgtgg
     8521 tcgactacac gagctcgaga tccttcagtc tcgtccgccc ggcgaggccg caatcatgga
     8581 cgattgcacg atcatgccgc ggcgtccagc cagcggtcca gcgatgcggc ggaagggccg
     8641 ctgatggaga tgatgcgcag gccggcccac tgatagccat tggtcgaggc gttttcgctc
     8701 cacatcgtat ggacgccggt ctcgatctcg cagggcgagc caccgcgccg atccggcagg
     8761 cggaagcgca attggtacag ggcgttgtcg tgcaggggcc gatggcagat cagcatcatc
     8821 ccgttgcgcg agaggttgcc aagccggccg atgggctcgc cgctgactgt gtcgatgact
     8881 tcgatgggcg ccatcgacgg tttgcgtggt gcgctgcggc gttcgtcgtt catgtcgccg
     8941 tctccgtggt caggttggta ccgctgaatt tgcgcaatcc tgtcaccagc gcatcccagg
     9001 cgcggtcgat catacccttg tcctgcggcg gcagcaggcg aacctggccg cgcgccatca
     9061 ggcgtgcgat ctgttccagc gtgcgctcgg gcgctgccgc gccgcgtgca ttcacgagca
     9121 ggcagcggcc gctgaccgga ttgtgccaag cgagtttgcg cgcgaccttc tggccttgct
     9181 ggttgatggt gaactccagc cagctcccga acggcagccg cttgagttca tcgagcgcgc
     9241 gccgctccga tggcgtcaag ggcagcttct gcggcgcagc cggcgcggcg ggcgccgctt
     9301 cgccttcgcc gcgcttgtgg cgcgacttca ggcggatcag cagctccgtc tggctgggcg
     9361 gtggcgtcgc ggcgcgcgaa ctcggccgct gcggcaggtc gagcgcgtag cgggtgatct
     9421 ggtcggcctc ggatgcctgc agcccgacct gggtcaagcc atggttgagg tcggcggcga
     9481 gcctggtgtc gtcgcgcccg accggggaat cgagcaactg gtcggcatcg gcgagcctgc
     9541 gccggtacgc ctcgctgttt tcgccctcgc gcagcagggt cagcgtgagc gcgtcggtcc
     9601 acgcgctttc gagcagcgcg cggaccaggg gcgtggtgtt gcgtccggcg agccgcttgc
     9661 tgatcgccgc cccggcggtg gtccgtgctt cgcgcaggcg gtcgcggcct tgcgcggctt
     9721 cgacctggcg gcgctcggcg atttcggcgc gccgcgagag gagccgcagg tgcgagtcga
     9781 gatcgtcggc ctcgcgttcg atgatgccga tgtcgccctg gaagtcgctg gtgatccgct
     9841 ggacgctccg ttgcagccgc tggttgagcg cggcgtcgaa gctgtcgctg ctgcggtcga
     9901 gccagaggtt cgcggtttcc aggaccaggt tcagcaggcg ccgtgcgggg tgctcgcgga
     9961 cggtgaagaa gttgctgtcg gacaacgcga tccgcaacag cgggacctgc aggctcgaga
    10021 gcaattgctg tgcgacgccc tcggcgcggg tttccgtcag caactggtcg tagagcagcc
    10081 cgatcaggtc gagcgcgtcg cggtgctcgg gttgcagttg cggacgcttg ccgcctgacg
    10141 cgccgcgttg cagttcggcc gccagttcct tggcgaggtt ctcgccggcg cctggcccga
    10201 cgcggcgcgg cggcagggcc tgcaacgcgg cgagcgcatc ctgcagttgc ggcatcgagg
    10261 cgaactcgcc cgattgcgcg gccgcggcgt cctcggggtg cagggtgcgg cggcgcgcat
    10321 ccagcaggtt gtgcaggtca cccgccgcgg gcggtggcgt gaaattgact gcgggcggaa
    10381 tcgatcgcgt ttccggcgcc tcgagcgggc tcggcgcatc cgccgaacgc tgctcggacg
    10441 cgccgcgcct gggtaggtag ctgcgcaggt cgggcaggat gccgcaggcc ttcaggtgtt
    10501 cgttgagccg gccgtagaaa gattccgccg ccccgaacag gcggcggtcg cacaaccggt
    10561 agaacagcag gcgctgttcg cgttcgatgg caagaccgtt cgcagcttcg cccagcaccg
    10621 cggccagcac ctgcggaccc gctggcagga cctcgctttc cagcggcggc gacgccagca
    10681 gcacgccgta gcggtagcag agctcgaaga tggcgctgcc ggcgtgcagc tcggtgcgcg
    10741 tggaaaggtc atccagcgcc atgctctcgt cgaactcgtg cgggtccacg agcgacaagc
    10801 cttcgctggc gagaggctgt gccgcacgcg ccgcggcggg cgcggggcgg ccgatcccgg
    10861 cgaagcggcg ttccagcgcc gccatcaagc ggccctggaa aagcgggcgc gactgcttca
    10921 cctcgcgcag cgcttccatg gcttcgtgct ggacttgtcc gccgctggat tgctcgccga
    10981 tccggaacag ctcgtgctcg aggtcgtcga gcacgttgcg cagcagcggt tccagcaatc
    11041 cgccgcattc tgcgagttgc tgggcgagca ggtgacgggc gcgtggcgcc aggtcgctgc
    11101 ggtccgcgag tgtcgggtgc ttttccatcg attcccccgc ggtccgcgtg tcggcacttg
    11161 ccggcgcacg cccgcgcacc gcggatatta cggaaccggc gcgcaaagcg ccatggccag
    11221 ggtcattcgt cgccggtccg cacgcgcacc gtcaccgcct cgccgtcgac ttcgacggtc
    11281 acctgcatcg gccggcagca caccgggcaa tcctcgatgt acgcggccga gccggccgac
    11341 gaatcgacga tgagttcgac ggattccccg caatacggac aaccgacggg cacgaaatca
    11401 ggcatggcgg tacctcctcg ccggctcggg caggaacgac gcgatcacgt cgttgaggaa
    11461 acggcgcccg agcggcgtgg tcctcaggaa tgccgggtcg tggaccaacc agccgcgatc
    11521 gcgggcgttg ccgagcggcg cgtcgatcgc ggacagcggc aagccggtgc gcacagtgaa
    11581 cgcctgcgcg gcgatgccgt ctcccaggcg caaggcattc aacatgaatt cgaacggcag
    11641 ttgttccgcc gcgacggcct cgtcaccgcc gacgcgctgc ccggtggcgg ctttctgcag
    11701 ataggcccgc ggcgaacgca ccttccagcg ccgttggacg gcgaactcgc cggttttccc
    11761 gggtccgggg tccccggtcc ccggtcccga gattttcccg tgcgcccccg cgccgatcgc
    11821 gaggtaatcg ccgaattccc agtagttgag gttgtgccgg cagcgacgac cgttgcgtgc
    11881 atacgccgac acttcgtatt gcgcgaagcc cgccgcggcc aaccgcgcat gacatgcctc
    11941 ctgcatgtcc caggccgcat cgtcgtccgg cagcgacggc gggcgcttgg cgaacggcgt
    12001 gccgggttcc agcgtcaact ggtagtgcga gatgtgcgcg ggctgcaggg ccaaagcgcg
    12061 ttccacgtcg gcaagcgcgc cctcgggtcg ttgctccggc agcgcgtaca tcaggtccag
    12121 gttgatgttg tcgtagccgg catcctgcgc gagcttcacc gcggtctcgg cctcggccgc
    12181 cgaatggatg cggccgagcg cgcccagctt ttcgtcgtcg aagctctgca cgccgaacga
    12241 gatgcggttg acgcccgcag cgaggtagcc gtcgaagcgg ccgtgttcga cggtgcccgg
    12301 gttggtttct agcgtaattt cgcaatcgtg cgcgaacgtc agccgcgccg cggcgccgtc
    12361 gaagatggcc gcgatcgcct ccggtggcat caggctcggc gttccgccac cgaagaacac
    12421 gctggcgatc tcgcgtcccg ccagcgcggt cccgaaatcc gcaaggtcgc cgtcgagatc
    12481 ggccatgagc gccgcgacgt attcgcgtac cggcagttcg ctgcgcaggc cgtgcgaatt
    12541 gaagtcgcag tacggacact tccgcacgca ccaggggatg tggatgtata gcgacaacgg
    12601 gaccggggag cggggaccgg gggccagcgg tgagccggca gagcggcccg acagcgagtc
    12661 ggcggtcgat ggcggacgct cgagacaagc ggacattcag cgtttcccgg tccccggttc
    12721 ccggttccgg gtcccgagta tttcgcgcag cgccgccagc gcctgcccgc ggtggctgat
    12781 ccggttcttt tcggcgggat cgagttcgcc cgacgaaagg ccgccgccgt cttcgggcag
    12841 gaacagcggg tcgtagccga agccgcgcgc gccgcggcgc cggcgcagga tgcgtccgcg
    12901 ccagcgcccg gtcgcgatca gcggatcggg atcggccgcg ctgcgcagca gtaccacgac
    12961 gcaggtgtag tgtgcggtgc gagcctcgtc gggcacgtcg gccagcgcac gcaacagctt
    13021 gtcgttgttg gcctcgtccg cgccgtgcac gcccgcgtag agggccgaat cgaggccggg
    13081 tgcgccgccc agcgcatcca cgcagatgcc tgagtcgtcg gccagtgcgg gcaagcccga
    13141 cgcgcgcgcc gcgtgccggg ccttcaggat cgcgttctcc acgaaggtgt ggccggtctc
    13201 ctcggcgccg ccgatgccga gttccgtttg cgggatgaat tcgatgccgc tgcccgccag
    13261 cagctcgcgc agttcggcga gcttgccggt gttgccgctg gcgaggacga tcctgttacc
    13321 catgacgatg cacttcggtg aagctagctt tcccggcgtg ccaatgcggc acgctgtttc
    13381 gcgatcagtt cgcccacgcc tttttccgcg agcgacaaca acgtgtccag ttcctcgcgc
    13441 cggaaggcgt ggccttcggc ggtgccctgc agttcgatga agccgccgcc gtcgttcatc
    13501 acgatattca tgtcggtgtc gcagtgggcg tcctcggcat agtcgaggtc gagcacgggc
    13561 acgccctgaa agatgccgac cgagaccgcg gccaccgcgc cgtggatcgg attgcgcttg
    13621 agcaagccgc gccgcatcaa tacgtccacc gcgtcgacca gcgcgacata ggcgcccgtg
    13681 atcgccgcgg tgcgggtgcc cccgtccgcc tgcagcacgt cgcagtcgag cgtaaccacg
    13741 cgttcgccga gggcgacgcg gtccacgcag gcacgcaacg agcgcccgat cagtcgctgg
    13801 atttccatgg tgcggccgcc ctgtcctccg cgcgcggctt cgcgctgggt gcggtcgctg
    13861 gtcgcgcgcg gcagcatgcc gtattcggcg gtgacccagc cctggccctt gccgcgcagc
    13921 cacggcggga tgcgttcctc gacgctggcc gtgcacagca cccgggtgtc gcccgcgctg
    13981 atcagcaccg agccctcggc gtggcgggtg tagtggcgtt cgatcgtgac cgcgcgcaac
    14041 tcgtcgggcg cgcgcccgga agggcgggtg gttgccatcg ggaaaggttt ccgcgcaggg
    14101 cccgacagtt taccatcgca ctttgccttt cccggaacga tgccgatgat tcgcagcatg
    14161 accgcgtacg cgagcgacga ggaaacaacg ccgctcggct ggctcgcttg cgaagtacgc
    14221 gcggtcaatc atcgattcct ggaaaccggc gtgcgcctgc ccgacgagct gcgcgcgctg
    14281 gagccgcagg ttcgcgaaca catcgcggca cgtctttcgc gcggcaagct cgatgcgacg
    14341 ctccgctatc gcgcgccttc cgccgcggcc gacctgctgc tggacgaacg caccgccatg
    14401 cagctctcca cggcggccga aaccctcaag gcgcgattcc cgcaactggc caccgatttc
    14461 gcggcgctgc tcgactggcc cggcctgctg gtcaagccgg agctcgacca ggagggcgtg
    14521 gcccaggcag ccctggcgct gctggaccgc acgctggacg aattcatcgc cggacgcgag
    14581 cgcgaaggcg agaagctcgc ggccgcgctg cgcgaccggc tcgatggcat cgaacacatc
    14641 ctcgcgggac tgcgcgcaat cctgccggac atccgcaagg cgctgcgcgc gaagctgcag
    14701 gcgcgcctcg gcgagctgca ggctgcggcg gatcccggca ggctcgagca ggaactcgtg
    14761 ctgcagctgt ccaggatcga cgtcgacgag gaactggatc gtctcgacgc gcacgtcggc
    14821 gaggcacgcc gggtgctgac gctgagcgag ccggtgggac gtcggctgga cttcctgatg
    14881 caggaattca accgcgaagc caacaccttg gcttccaagt ccgcggacca gcgcacgact
    14941 cgcgcggcgg tcgatctcaa ggtgttgatc gagcagatgc gcgagcaggt gcagaacctc
    15001 gaatagtgcg gcgtttcgca agcggtggca aagcgccgcg aggttggccc gaatcggggc
    15061 acgcaacagg ttcgcagtcg ccaacgagtc cgggtggccg cagacgcttg agtgaggcgg
    15121 acgttcttgc ggttcgaaac gcaagccctg gggcatcctg gtgcacggtg ctgctgtgcg
    15181 gtgtgtcaca aattgcagcg gatcgcgtga tcgcggctgc aatgggcgac tgcgttgctc
    15241 gtcgtccgtt cctcggccgg catgaatctt gcgtgccaaa cagcagcaat gaatcccggg
    15301 gaccacttgt aagtcatgaa cctgatccac cgtgtgtgct ggaaccgccc gtctgaccaa
    15361 cgggttccgg caccggagct tgcgcgttgt cgtcggaaag gaccgacacg gaggatggcg
    15421 ggatgtcgcg ggctggtccg ctcgctgctg tgggtgtcgc tggccgtggc gggggtcgcg
    15481 cacgcgggca atgctccgac gggcggacag atcgcggcag gctcgggcca gatccagcag
    15541 tcgggcaaca ccaccacgat tcgacaggac agccagacgc tgtcgttgaa ttggcagagt
    15601 ttcgacatcg gcgccgcgca aacggtgcaa ttcctgcagc ccggtgccga ctcgatcgcg
    15661 gtgaaccgca tcctcggcag taccgccagc gaaatccagg ggcatctcga ggcgaacggc
    15721 caagtgtggc tgatcaatcc gaatggcgtg ctgttcggcc gcgatgcaca ggtcaatgtc
    15781 ggcggcttgg tcgcctccac gctggcggtg gacgattcga cgatcgggtc cggtgatgtc
    15841 cgcttcgccg gcaacggcaa gggcaaggtc gtcaaccggg gccgcatcac cgccgccgat
    15901 ggcggctacg tggctttgct cggcaaccag gtatccaacc agggcgtcat ccgcgcccgc
    15961 ctcggtacgg tcgcgctggg tggtggcacc gccgtcaccc tcaccttcgc cgaccatcac
    16021 ttgatgcacc tgcaggtgga cgcgaacgcc gttcgcagcc tggtcgaaaa ccgccaattg
    16081 atcgtcgccg acggcggcgc cgtgctgatg accgccggtg cccgcgattc gttggtggcc
    16141 agcgcggtga acaacagcgg caccgtgcag gcgcgcagcg tcgaggaaca cgacggcacc
    16201 gtcacgctgc tgggaggcat gcaagccggc agcgtgcagg taggcggcac gctcgacgcc
    16261 agcgcccccg atggcggcga tggcggccag atcgaaacct ccgctgcgca tttcgagctg
    16321 gccgacgatg cacgcatcac cgccgccgcg gccggcggca aggccggcac ctggctggtc
    16381 gacccggtcg acctcacgat cgacgccgcc gcggcgtcca ccatctccgg cacgctggag
    16441 ggcggcacca acgtcatcga gcaaaccacc gccaccggcg ccaccggtac cggcacgaag
    16501 acctcgggca acggcgacat caacgtcgac gccgcgctga gctggaccaa cccgtccgcc
    16561 acgctcaccc tggacgcgta ccacgacatc aacgtcaacg caccgatcaa cggcagcggc
    16621 ggcatcgtca tgaaggctgc gaatggcagc gtgacgatcg gcagcggcgg cagcctgcaa
    16681 gcgggcagcg gtgcgacc
//
LOCUS       DASSNH010000250         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_18721, whole genome shotgun sequence.
ACCESSION   DASSNH010000250 DASSNH010000000
VERSION     DASSNH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18721"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..>2387)
                     /locus_tag="VFK00_09260"
     CDS             complement(338..>2387)
                     /locus_tag="VFK00_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HET7593178.1"
                     /translation="AYAGGYSSAAIGAYATALGDSSVALGNTSYASAGYSTAVGPFAV
                     AQGYSSTAVGDTANALGDDSTAIGHYATAEGASSSALGDNARASGDYSTALGSGADTM
                     GYSNSVALGAGSVVDRENSVSVGAPGAERQVTNVADATEDHDAVNLEQLTDAVNSGPG
                     ADTAHYFKANGLGDGSDDAVAIGTQAVAAGPNAYASGANSVAVGAGSQAGGTNASAVG
                     SGAIATGLNATAVGAGASATRANASAFGAGAIAGGIGSSAVGAGSLAGGNNASAFGAG
                     ATASNSRSSAFGAGAIATGSGAVAFGAGAQASGNNSVALGAGSLASFANTISVGSAGF
                     FRRVVNVADPVNAHDAVTLQYLQNNYVAKSDVTTSAAPAQGGTMDASAMMVQDAGSSK
                     VSGTSAANAATGAPIAAADTTSVAAGTGTATTQAMPADAAGTGTPVANSAVTGTPVAS
                     AANAPATGTGGSTVSPAAAGTPVATAAGGTAAATSAPAAATGTGSATVATAGTGSQSV
                     NGAVAAGANGGAPGAVPFGNPANAPQSQDAVATANSYTDQQTQEALQSAQVYTDASTA
                     RAVTQANAYTNDVLTGYVTTDTFNAYQQQVNSRFAIQDKRIDTLGAMAAASTQMAINT
                     AGLSGQNRVGVGVGTYGSRNAASLGFQHVFSNNNASISVGVSSADGETSGGVGAGFSW
                     "
BASE COUNT          343 a          859 c          776 g          409 t
ORIGIN      
        1 ccgccggccg gtgcggcggc aactgccgtg cccgcgacgc ccaatgcgcc cgcaacggca
       61 agtgtaagaa cccggctacg caaacctgca cgatttttga acatgagttg ttcctctcca
      121 cggggtttcg aagggcatcg aaaaatcgat gcgttcactg ctgcgccatc aacaaaatcc
      181 gttccaccgg atccatgcac tccccctgtt tttgctgccg ccgcacctcg gttccggaat
      241 gcggcgattt cttgcctctc catcggtccg cgccgggcca ttaccggccc aacttggccg
      301 cgggtacacc ccacccgcgg tccgcaccac gcgcgactta ccagctgaag ccggcgccca
      361 cgcctccgga cgtttcgcca tcggccgagg acacgcctac cgagatgctc gcgttgttgt
      421 tggagaacac atgctggaag ccgagcgaag cggcgttcct gctgccgtag gtaccgacgc
      481 cgacgccgac gcgattctgt cccgacaagc ccgcggtatt gatggccatc tgggtggagg
      541 ccgcggccat ggcaccgagc gtgtcgatcc gcttgtcctg gatcgcgaag cgcgagttca
      601 cctgctgctg gtaggcgttg aacgtgtcgg tggtgacgta gccggtcagc acatcattgg
      661 tgtaggcgtt ggcttgggtc acagcacgcg ccgtcgaggc atcggtatag acctgggccg
      721 actgcaaggc ctcctgcgtc tgttggtccg tgtaggagtt ggccgtcgcg acggcatcct
      781 ggctctgcgg cgcgttcgcg gggttgccga acggcacggc accgggcgcc ccgccattcg
      841 cgccagcggc cacggccccg ttgacgctct gggaacccgt gcccgccgtc gctaccgttg
      901 cgctgcccgt tccggtggcc gccgcaggcg cgcttgtcgc ggcggctgtt ccgccggccg
      961 cggtcgcgac cggcgtacct gcagcggcag gactgaccgt ggagcctccg gtgccggttg
     1021 ccggggcgtt ggccgcgctc gcgaccgggg tgcctgtgac ggccgagttc gcgaccgggg
     1081 taccggtccc ggcagcgtct gccggcatcg cctgcgttgt cgcggtaccg gtcccggctg
     1141 ccaccgacgt cgtgtcggca gcggcaatcg gggcaccggt ggcagcgttg gcggccgagg
     1201 tcccggaaac cttcgagctg ccggcgtcct gcaccatcat tgccgacgcg tccatggtgc
     1261 caccctgcgc cggcgcggcg ctggtggtca catccgattt cgccacataa ttgttctgca
     1321 ggtactggag cgtcaccgcg tcatgtgcgt tgaccggatc ggcgacgttg acgacgcgcc
     1381 ggaagaatcc cgccgaaccc accgatatgg tgttcgcaaa cgaggccagc gaacccgcac
     1441 cgagtgccac gctgttgttg ccgctggcct gggcaccggc gccgaacgcc accgcaccgc
     1501 tgccggtggc gatcgccccc gcgccgaagg cggacgacct gctgttgctc gcggtcgcgc
     1561 ccgcgccgaa tgccgacgca ttgttgccgc cagccaatga gcctgcaccg actgccgacg
     1621 atccgatgcc gccggcgatc gcaccagcgc cgaaggccga agcattggcg cgggttgccg
     1681 atgcgccggc gcccacggcg gtcgcattga gtccggtcgc gatcgcaccg ctgcccacgg
     1741 cagatgcatt ggttccgccc gcctggctgc ccgcgccgac cgcgacgctg ttggcgccgg
     1801 aggcgtacgc gttgggaccc gccgccacgg cctgtgttcc gatcgctacc gcatcgtcgg
     1861 aaccgtctcc caagccgttg gccttgaagt agtgcgctgt atcggcaccc gggccgctat
     1921 tgacggcatc ggtcaactgt tccaggttga ccgcgtcatg atcctcggtg gcgtcagcca
     1981 cgttggtgac ctggcgctct gcacccggcg cacccaccga gacgctgttc tcgcgatcca
     2041 cgaccgagcc cgcacccagc gctaccgaat tggaatagcc catggtgtcg gcgccgctgc
     2101 caagcgcggt gctgtagtca cccgaggccc ttgcgttgtc gcccagggca ctcgaacttg
     2161 cgccttcagc cgtcgcgtaa tgcccgatcg ccgtcgagtc atcgcccagc gcattggccg
     2221 tgtcgcccac cgccgtcgag gaatacccct gcgccaccgc gaacggaccc accgccgtgc
     2281 tgtagcccgc cgaggcatac gaggtgttgc ccagcgccac gctcgagtca ccgagtgccg
     2341 tcgcatacgc gccgatcgcc gcgctcgagt agccgcccgc gtacgcg
//
LOCUS       DASSNH010000251        21240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1492962, whole genome shotgun sequence.
ACCESSION   DASSNH010000251 DASSNH010000000
VERSION     DASSNH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 21240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21240
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492962"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..682
                     /locus_tag="VFK00_09265"
     CDS             17..682
                     /locus_tag="VFK00_09265"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7593179.1"
                     /translation="MAFEDYDEYEQGEAVRKWIRENGIAVAVGVVLALLLIFGYRQWQ
                     AHRTNHHVQAAAQYAVVQNAVQRDDKQAMAAALGELQKEYADTPYAALGSAAVAEYDV
                     GKDDLKPAAQNLQWAVSHSGQAALKSLFTLRLARVLIAQGQAKQALDALDGIPAGDYA
                     AIAAELRGDAQLKLGNADAARKAYSQALAKLDKDAPQRNAVQMKLDNLTQPAAAPTGK
                     PAT"
     gene            679..1908
                     /gene="bamB"
                     /locus_tag="VFK00_09270"
     CDS             679..1908
                     /gene="bamB"
                     /locus_tag="VFK00_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HET7593180.1"
                     /translation="MKRRHLLILPLLLATVVLSGCGLWNKIFNRGENIQPPKPLVEFP
                     ASVQVQEVWSTSIGGGAGRSGVRLQPAYADGKLYVISTDGKIEALDAATGKSVWEQST
                     GTRGGIWPFRHDKAGVDARYAGGPAVSGSLLVVGTLDGHVYAMDAATGKQLWSAEVDN
                     EVISPPAIDAGTVYVRTNSSYVYAFDANTGERKWLNDQGNVPLLSLRGNGPLLAGHGV
                     VMFGTDDGNLQALLADSGASVWKIPVTKGLGRTDIQKLNDADDALQLDGTTLYATAYH
                     GDLVAVDASQGQVLWDRPFSSYVGVAVGDDELVGVDDDSLVWAFSKNGGGDMWKQDAL
                     QYHWLTAPAIQGQYAVVGGIEGYVHWLDLSDGKLAARMRMSKDPIRARPVVNGDMVYV
                     EDVDGRITAYRISGNAG"
     gene            2132..3532
                     /gene="der"
                     /locus_tag="VFK00_09275"
     CDS             2132..3532
                     /gene="der"
                     /locus_tag="VFK00_09275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7593181.1"
                     /translation="MLPVVALVGRPNVGKSTLFNVLTRSREALVADLPGVTRDRHYGV
                     CRLGARPFVLVDTGGLSGERVEMDALTERQVQVAIDEASVCVLLVDAREGLLPQDRAI
                     LDRLRRTGKPVLLAVNKTDGVDVATTLAEFSALGVAATFAIAAAHGRGSDALLAGILP
                     LLPADLDAGEAEGESPSGTRIAIVGRPNVGKSTLVNRLLGEERVVVSDIAGTTRDSIH
                     VPLERDGRQYTLIDTAGVRRRARVEDAIEKFSVIKTLQSIAAAEVAVVMLDAGEGVTD
                     QDLALIGQVLDAGRALVIAANKWDGLEQYDRERCKAGLERKLDFVAWAKIVFLSAKHG
                     SGLRELMRAIDRAGAAASRELGTSDLTRALEKAVAAHQPPLVRGHSPKLRFAHPGGDH
                     PPVIVIHGTRTKHIADSYRRYLENFFRKRYKLEGTPIRIEFREGENPYAGRRNVLTEA
                     QQRKRRRMVRHVRSRR"
     gene            3529..4161
                     /locus_tag="VFK00_09280"
     CDS             3529..4161
                     /locus_tag="VFK00_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7593182.1"
                     /translation="MSGIGTLGVILAGGAGSRVGGADKGLLAVHGAPVVEHVAAALRP
                     QCDDMLIVANRNRPDYARVARVVGDEIPGHAGPLAGIAAALALVVESGENPFEDCRWL
                     LTVPVDCPDFPGDVGDRLRGAFDGDARLACAYARDERRLQPLFALYSLASGGALLESA
                     RAAASLHGSVLRWHMGLAARAVEFRGQAAAFHNLNAPEDFREYERSHAAI"
     gene            4148..5386
                     /gene="glp"
                     /locus_tag="VFK00_09285"
     CDS             4148..5386
                     /gene="glp"
                     /locus_tag="VFK00_09285"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET7593183.1"
                     /translation="MPQSDYPTRLSLGDALAIVATRAAQHRLPVETVDLATAHGRVLA
                     KDIHAPHPLPPFDNSAMDGFAVRGEDLPAKGERQFRLTGEVFAGATAAPALEAGECIR
                     VTTGAPMPPGADTVVIKENAVVDVGEVTLKAGEKAGANVRSAGEDYARGDVALMAGTS
                     LHAPQVAVLAALGVAEVSVRRQPRIAVIATGNELVPAGQPLGFGQIHESNAVMLAALG
                     REAGAPIATQEVVRDDPGALRGALLRAAADADIVVTSGGVSAGEADHLPRVLQAVGEI
                     HFHKVRLKPGMPTLFGQIGASLYFGLPGNPVASAVTFRVFVGFALRSMLGITKVSEPR
                     RARLDAPLHKRHARAEFARCSLRSDEHGALWATLHARQGSGMLRGLAETEALALLPEG
                     TREFAEGDVVMLWPEHFWHG"
     gene            5594..6718
                     /gene="moeB"
                     /locus_tag="VFK00_09290"
     CDS             5594..6718
                     /gene="moeB"
                     /locus_tag="VFK00_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET7593184.1"
                     /translation="MTVREISPQEALQRQQAGALLLDVRETYEHATGMAEGAIALPLG
                     EVLQRIGDVAPDSTREILAICAHGQRSLRAAQALQQRGYADVVSVAGGTVRWIEEGLP
                     TSKPGGDEDFFDRYSRHLRLPEVGEAGQRKLESARVVLLGAGGLGSPAAYYLAAAGVG
                     TLVIADDDVVERSNLQRQILHTDDRIGVSKLQSAAHALHGLNPRVDIEGLPERVTSAN
                     VDALLEGADVVLDGADNFPVRYLLNDACVKLAIPLVYGAVQRFQGQVGVFDAGRRRGV
                     APCYRCLFPQPPAPEDAPNCSEAGVLGVLPGVVGMLQATEVIKLILGIGEPLVGRVLM
                     FDALAMRFHETRLAPDPECPVCAPGKPFPGYIDYERFCTG"
     gene            complement(6851..7150)
                     /locus_tag="VFK00_09295"
     CDS             complement(6851..7150)
                     /locus_tag="VFK00_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HET7593185.1"
                     /translation="MSSPDRIQLEAAAFRRLLRHLNQDRLDVQNIDLMILAGFCRNCL
                     ADWYRAAAAERGSEMTKDQAREAIYGMPFAEWKAKYQKDATPEQLAAFEAAPKKG"
     gene            7223..8095
                     /gene="folD"
                     /locus_tag="VFK00_09300"
     CDS             7223..8095
                     /gene="folD"
                     /locus_tag="VFK00_09300"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET7593186.1"
                     /translation="MPETAHNARILDGRRIADEVLEQVATGVAARLAAGRSAPGLAVV
                     LVGADPASAVYVRNKRKACKRAGFRSFDYDLPAETPEAELFALVDRLNADPEVHGILV
                     QLPLPPHIDASALIDRIDPRKDVDGFSAINLGHLALRRFGLRPCTPKGVMTLLGRTDR
                     PVRGQHAVVVGVSNHVGRPLVLELLIAGCTTTCCHKFTQGLDQHVARGDIVIAAVGKP
                     GIVKGEWIKPGAVVIDVGINRMQDGHLVGDVEFEEAAKRASWITPVPGGVGPMTVATL
                     MENTLEAAQALDGP"
     gene            8277..9734
                     /gene="guaB"
                     /locus_tag="VFK00_09305"
     CDS             8277..9734
                     /gene="guaB"
                     /locus_tag="VFK00_09305"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7593187.1"
                     /translation="MRILAEALTYDDVYLVPAHSSVVPRDVDTSTDLTRAIRLNIPLM
                     SSAMDTVTEARLAITMAQTGGIGIIHKNMTVERQAAEVRLVKKFEAGVIRDPLTVGPT
                     TSIGEVMHITRSHNISGVPVVEEGNKLVGIVTSRDLRFEKKPDDPVKNIMTRKDKLVT
                     VQEGAGEDEIVGLLHKHRIEKVLVVNDAFELRGLITVKDIQKARDNPHSAKDDHERLR
                     VGAAVGVGGETERRVAALAEAGVDVIVVDTAHGHSQGVIERVAWIRKNFPGLQMIAGN
                     IVTGDAAKALRDAGVDAVKVGVGPGSICTTRIVAGVGVPQITAISMVADALKGDIPLI
                     ADGGVRYSGDVAKAIVAGASCVMLGSMFAGTEEAPGEIELYQGRSYKSYRGMGSLGAM
                     QSGSSDRYFQEQAAADKLVPEGIEGRVPYRGPLRAIVHQLVGGLRASMGYCGCANIEQ
                     MRTMPQFVRVTGAGTREAHVHDVQITKEAPNYRLD"
     gene            9876..11423
                     /gene="guaA"
                     /locus_tag="VFK00_09310"
     CDS             9876..11423
                     /gene="guaA"
                     /locus_tag="VFK00_09310"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7593188.1"
                     /translation="MSNIHTDKILILDFGSQYTQLIARRIREIGVYCEIWAWDHDPSE
                     IAKFAPKGVILSGGPDSVVEAGSPRAPGDVFALDVPILGICYGMQAMAVQLGGNVEGG
                     HAREYGHAIIATESCALFEGTPARQDVWMSHGDRVTALPTGFALAASTESVPIGAMAD
                     EAHRWYGIQFHPEVTHTEHGMAILRRFVVDICGCATLWTPARIIDDAIAQVRGTVGDD
                     RVLLGLSGGVDSSVVAALLEKAIGDQLTCVFVDTGLLRAGEGDQVMDTMARHMGVHVI
                     RVDASDRFFAALEGIADPEAKRKIIGRLFVEVFDEESHKLQGVKWLAQGTIYPDVIES
                     AGGKTGKAHVIKSHHNVGGLPEKMHLELLEPLRELFKDEVRRIGVELGLPREMVYRHP
                     FPGPGLGVRILGEVRREYADILKAADHIFIEELRAADLYDKVSQAFAVFLPVRSVGVV
                     GDGRAYEWVIALRAVETIDFMTAHWAHLPYEFLGRVSTRIINELRGISRVVYDISGKP
                     PATIEWE"
     gene            11535..13052
                     /locus_tag="VFK00_09315"
     CDS             11535..13052
                     /locus_tag="VFK00_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET7593189.1"
                     /translation="MSTSKDEPEAELPRISTGSNGLDLILGGGFDPQRMYLYEGQPGA
                     GKTTLALQFLLEGVRRGERTLYITLSETERELELVAQRHGWSLAGVDVFELVPSETTL
                     DPSQELTILQPSEMELSETSQLIFDKVEELNPSRIVIDSLSELRLLAQSALRYRRQVL
                     ALKHFFSSRKSTVILLDDLTAQQKDLQLHSIAHGVVLLEQLAIDYGSERRRLRVVKMR
                     GIPFAGGFHDFTIEHGGLRVYPRLVAAEHHKDFAGAYVPSGNRELDMLLGGGLERGTN
                     ALLIGAAGVGKSSIAVTYAIAAAQRGEHTMIFAFDEGRGTINARAKSLGLPFEEAIES
                     GMIKLQQIDPAELSPGEFAQLVRQSVETEEARIVIIDSLNGYLNSMPDERFLVLQMHE
                     LLSYLSQLGVLSILVLAQHGLVGAMQTPVDISYLSDAVLMLRYFEYDGTVRRALSVVK
                     KRSGHHEHTIREFRLSHQGVRLGPPLKGFNGILSGTPIYTGDRSPLLADEDSRAG"
     gene            13057..14715
                     /locus_tag="VFK00_09320"
     CDS             13057..14715
                     /locus_tag="VFK00_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7593190.1"
                     /translation="MDARVLILAPVGRDAGACARLVAQAGLVPQACSDMAELVAQLDL
                     GADMVLLTEEALYGTALDPLAGWVASQPPWSDLPFIVMTSHNEGPRFARFRRDLVRKL
                     RNVSFLERPMQAISLQAAVLSAARGRARQYEARAYLEAQRNAAEDLERLVAQRTEALQ
                     AANEQLREESRRRERAQAALMQAQKLETMGQLVGGVAHDFNNLLMAVIGNLELLAKRI
                     DHDPRQARLLRGAMEGARRGATLTQRLLAFARKQELQFRATDVLALVKDMRDLIARSV
                     GPLIDVQIRASEPLPPVVGDPNQLEMALLNLAVNARDAMPSGGVLTISLEQCVATAEQ
                     SELKPGVYVKLSVADTGEGMDAATLARAVEPFFSTKGVGKGTGLGLSMVHGLAEQSGG
                     TFRLESRQGVGTKASLWLPLAHSELEVAAPPERLGTTAAAAIIVLVDDDALIATSTAA
                     LLEDLGHQVTEFRSGQEALAAIRGGLRPDIVITDHAMPGMTGVELAIALRELEPELPI
                     LLATGYAELEGAPRIDLPRIAKPYTQQQLSVEIERLLSQRVHST"
     gene            complement(14707..15237)
                     /locus_tag="VFK00_09325"
     CDS             complement(14707..15237)
                     /locus_tag="VFK00_09325"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7593191.1"
                     /translation="MHDTLQRLFRYKACADDALLDALARLDGKSPVTELAIKALGHSY
                     IVDRIFAAHLRGKPHGYTAVNPAETPTLEGLSADIRESDREYVDYVPTLDADQLMERI
                     DFAFTDGAPARMSREEMLVHVINHGTGHRGQVSAVMLLNALAPARDGFTTYLHEAEAS
                     TRARAPGQGHPCSKSG"
     gene            15358..15984
                     /locus_tag="VFK00_09330"
     CDS             15358..15984
                     /locus_tag="VFK00_09330"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7593192.1"
                     /translation="MARKPPTKPRKTASQARSRATVEALLGATARILVREGFDKASTN
                     RIAEVAGVSVGSLYQYFPGKEALVAAVMERHQHEIMQEVRDGLAKLFALPVEQAMREL
                     VALAVKAHRVDPALHRVLTEQIPRVGKLEEREAFDRGNFTLFRTYLERNRDELHVRDL
                     DLATFVCVTSIEALTHNAVLHYPAALPAQAMESLVEEGARLVTGYLKA"
     gene            complement(16024..16653)
                     /locus_tag="VFK00_09335"
     CDS             complement(16024..16653)
                     /locus_tag="VFK00_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HET7593193.1"
                     /translation="MKRSSAEILREYGPFPDAERVHGVAWDGERVWFASGDKLNAVDP
                     ASGRPVRSIDVAAHAGTAFDGKHLFQIAEDRIQKIDPANGRVLSTIPAPGDGGNSGLA
                     WAEGTLWVGEYRARKIHQIDPETGKILRTIECRRFVTGVTWADGDLWHGTWEEDESDL
                     RRIDPVSGEVLEQIDMPEGTTVSGLETDGNGQFLCGGGSSSRIRVVRRH"
     gene            complement(16687..17907)
                     /locus_tag="VFK00_09340"
     CDS             complement(16687..17907)
                     /locus_tag="VFK00_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7593194.1"
                     /translation="MDSLITAAARALAAGDPLGALKRVALREDAPALALRGIALAQLG
                     DFERAKALLRRATRAFGRRETVARARCVVAEAEVALASRELGWPERRLEAAQDVLEAH
                     GDRVNAAHARYLAIRRLLLIGQLDQAEGALAAFESAPLPPALRAVHELTAAGIAVRRV
                     RAGTARAALARAGRAARGAGVPALLAEVEYAQRMLDAPAARLIGRGGERFLRLDEVEA
                     LFASEALVVDACRRTVRDGRATVSLASRPVLFALAQALAEAWPHDASRHVLITTAFHT
                     KFADETHRARLRVEIGRLRAALRSFAHIRATRRGFAVVPARAREVAVLARPTEEKHAA
                     VLALLADGQPWSSSALAMALGMSQRHLQRALDALAADAKIQPVGRGRARRWATPSLPG
                     FTTALLLPAPLPGD"
     gene            18043..18669
                     /locus_tag="VFK00_09345"
     CDS             18043..18669
                     /locus_tag="VFK00_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET7593195.1"
                     /translation="MTTHKTGTRDEWLEERLRLLDAEKELTRRSDELAQRRQELPWVR
                     IEKPYHFETDDGEASLADLFRGRSQLLVYHFMFGPDYTAGCPSCSMIADGFNGFTGHL
                     ANHDVMLWAVSRAPLPKLKAYERRMGWTFPWASSFGSDFNFDFAVGITEMQQREGGAE
                     YNYRREAATSSGGTPDGPSTKFGSESPACASHDRAGRILRRTCVNVRA"
     gene            18666..18836
                     /locus_tag="VFK00_09350"
     CDS             18666..18836
                     /locus_tag="VFK00_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET7593196.1"
                     /translation="MSAFVLDNGVVYHTYSSYSRGLDGLWGTYQWLDRAPRGRNEEGG
                     IWWRRHDEYGRS"
     gene            18836..19234
                     /locus_tag="VFK00_09355"
     CDS             18836..19234
                     /locus_tag="VFK00_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593197.1"
                     /translation="MRCADTRGAVAWLHLASTPTFVAMAVANAAMPGAGMDMLCPALN
                     GVTPLPGSMAMMYLLMGAFHAGPWLRMFIVRRKRLRRSGFPGGRERGPARMNAYICPA
                     ATKVQATADARNPAPFMQETKALDAAPSSE"
     gene            19522..20001
                     /gene="tadA"
                     /locus_tag="VFK00_09360"
     CDS             19522..20001
                     /gene="tadA"
                     /locus_tag="VFK00_09360"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET7593198.1"
                     /translation="MTDHPTPPLREDDVAWMRRALELAIHARDADGEVPVGAVLVLDG
                     EIVGEGWNRNITLNDPSAHAEIGALRDAGKRLGNHRIPGSTLYVTLEPCAMCAMALVH
                     ARVARVVYGAADPKTGAAGSVFDTLVSERHNHRVAVEGGLLAEESAELLRGFFRAKR"
     gene            20169..20924
                     /locus_tag="VFK00_09365"
     CDS             20169..20924
                     /locus_tag="VFK00_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7593199.1"
                     /translation="MARYRERYGQEDHGVGDAARRHAEADYRDQFGTGGRTQNLPDDY
                     GTGRDPQAFERRYRDEPYYEERYGEGESRFPGGGWSSHHRGGGRPGWRGDAGFGRTYS
                     GNYESLGRREPLRYGEGGGQAMARGYRGRGPRNYTRSDERIQEDISERLWDADDVDAS
                     DVTVEVKNGEVTLRGNVERRSVKHRIEDIADGCSGVKDIHNEIRVQPRNEWPYDKGVA
                     AGSIDDASRRSGNGERNEGMVQSAIEEMPPRSH"
     gene            complement(21027..>21240)
                     /locus_tag="VFK00_09370"
     CDS             complement(21027..>21240)
                     /locus_tag="VFK00_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7593200.1"
                     /translation="LLEYLKVSRLVLTGVAGDQCVMTTAMDAHIRDYHLWVPANATAS
                     LTPKRNQRALDHISEALGASVAPVDA"
BASE COUNT         3590 a         7010 c         7239 g         3401 t
ORIGIN      
        1 tgtgcgtgga tgaaccatgg ctttcgaaga ctacgacgaa tacgagcagg gcgaagccgt
       61 ccgcaagtgg atccgggaga acggcatcgc cgtggccgtc ggcgtggtgc tcgcgctgct
      121 gctgatcttc ggataccgcc agtggcaggc gcacaggacc aaccatcacg tccaggcggc
      181 ggcgcagtac gccgttgtgc agaacgcggt gcagcgcgac gacaagcagg ccatggcggc
      241 cgcgctcggc gagctgcaga aggaatacgc cgatacgccg tatgcggcgc tgggcagcgc
      301 cgcggtcgcc gagtacgacg tcggcaagga cgacctgaag ccggccgcgc aaaacctcca
      361 atgggcggtt tcgcacagcg ggcaagccgc attgaaatcg ctgttcacgc tgcggctggc
      421 gcgggtgctg atcgcccagg gccaggcgaa gcaggcgctg gacgcgctgg atggcatccc
      481 ggccggcgac tacgccgcga tcgccgcgga acttcgcggt gatgcgcaac tcaaactcgg
      541 caatgccgac gccgcgcgca aggcctactc gcaggcgctg gccaagctcg acaaggacgc
      601 gccccagcgc aacgcggtgc agatgaagct cgacaacctc acgcagccgg cggctgcacc
      661 gaccgggaag cccgccacat gaagcgtcgc cacctcctga ttttgccgtt gctgttggcg
      721 acggtagtgc tttccgggtg cggactctgg aacaagatct tcaaccgcgg cgagaacatc
      781 cagcctccca agccgctggt cgagttcccg gccagcgtgc aggtccagga ggtatggtcg
      841 accagcatcg gcggtggcgc cggccgctcg ggcgtgcgcc tgcaaccggc gtatgcggac
      901 ggcaagctgt atgtcatcag caccgacggc aagatcgagg cgctcgacgc cgccaccggc
      961 aagagcgtgt gggaacaatc gacgggcacc cgcggcggca tctggccgtt ccgccacgac
     1021 aaggccggcg tcgatgcacg ctacgccggc ggtccggccg tgtcaggcag cctgctggtg
     1081 gtggggacgc tcgacgggca cgtgtacgcg atggacgcgg cgaccggcaa gcagctgtgg
     1141 agcgccgaag tcgacaacga ggtgatttcg ccgcccgcga tcgacgcggg cacggtgtac
     1201 gtgcggacca actcgagcta tgtgtacgcc ttcgatgcca ataccggcga acgcaagtgg
     1261 ctcaacgacc agggcaacgt gccgctgctc agcctgcgcg gcaatggtcc gctgctggcg
     1321 ggtcacgggg tcgtgatgtt cgggacggac gacggcaacc tccaggcgct gctggccgac
     1381 tccggcgcgt cggtctggaa gattcccgtc accaagggtc tggggcgcac cgacatccag
     1441 aagctcaacg acgccgacga cgcgctgcag ctcgacggca ccacgcttta cgccacggcc
     1501 taccatggcg atctggtcgc agtggatgcc tcgcaagggc aggtgctctg ggatcgtccg
     1561 ttctccagct atgtcggagt cgcggtcggc gatgacgaac tggtcggcgt cgacgacgat
     1621 tcgctggtgt gggcattctc gaaaaacggc ggtggcgaca tgtggaagca ggacgccctg
     1681 cagtaccact ggctgaccgc gccggcgatc cagggccagt acgcggtggt cggcggaatc
     1741 gaaggctacg tccattggct cgacctgtcc gacggcaagc tcgcggcacg gatgcggatg
     1801 tccaaggacc cgatccgtgc gcggccggtc gtcaacggcg acatggtgta cgtcgaagac
     1861 gtcgacggac ggatcacggc gtaccggatt tccggaaacg ccggctgaag cgcccgcaaa
     1921 cggcgtcgca acattaccca tgtaggagcg gcccaaggcc gcgacgggct gccgcttcgt
     1981 tttgcgttcc gctcgcgcgc ctgcgcgcgg tctcgatacg tacgagaccg cgttggctcg
     2041 gatcgaggcc atgggacgct cccacatgta cactcgggcg tcgcccgcgg cggctccgcc
     2101 cgcacgcttc gcgagtctgg attcctgacc catgctgccc gtagtcgccc tggtcggccg
     2161 ccccaacgtc ggcaagtcca cactgttcaa cgtgctgacc cgcagccgcg aggcgctggt
     2221 cgcggacctg ccgggcgtca cccgtgaccg ccactatggc gtgtgccgcc tcggcgcgcg
     2281 tccgttcgtg ctggtcgaca ccggcggcct gtcgggcgaa cgcgtcgaaa tggacgcgct
     2341 gaccgaacgg caggtgcagg tggcgatcga cgaggccagc gtgtgcgtgt tgctggtcga
     2401 tgcgcgcgaa ggtttgttgc cgcaggaccg cgcgatcctc gatcgcctgc gccgcaccgg
     2461 caagccggtg ctgctcgccg tcaacaagac cgatggcgtg gacgttgcga cgacgctcgc
     2521 agaattttcg gcgctgggcg tcgccgcgac cttcgcgatc gcggcggcgc atgggcgtgg
     2581 gagcgatgcc ttgctggcgg ggatcctgcc gttgctgcct gccgacctcg atgccggcga
     2641 agccgaaggc gaaagcccgt cgggcacccg catcgcgatc gtcggccgcc cgaacgtcgg
     2701 caagtccacg ctggtcaacc ggctgctggg cgaggagcgc gtggtggttt cggacatcgc
     2761 cggcacgacc cgcgactcga tccatgtgcc gctcgagcgc gacggcaggc agtacacgct
     2821 catcgatacc gccggcgtac gccggcgcgc gcgcgtcgag gatgcgatcg agaaattcag
     2881 cgtcatcaag acgctccagt cgatcgccgc cgcggaagtg gccgtggtga tgctcgacgc
     2941 gggcgagggc gtcaccgacc aggacctggc gctgatcggg caggtgctcg acgccggccg
     3001 cgcgctggtg atcgcggcca acaagtggga cggcctcgag caatacgatc gcgagcgctg
     3061 caaggccggg ctcgagcgca agctagattt cgtcgcctgg gcgaagatcg tgttcctctc
     3121 ggccaagcac ggctccgggc tgcgcgagtt gatgcgggca atcgatcgcg ccggggcggc
     3181 ggcgtcgcgc gagctcggca cctcggatct cactcgcgcg ctcgaaaagg cggtggccgc
     3241 gcaccagccg ccgctggtcc gcgggcactc gccgaaactg cgcttcgcgc atccgggcgg
     3301 cgaccatccg cccgtgatcg tgatccacgg cacccgcacc aagcacatcg ccgacagcta
     3361 ccggcgctac ctcgagaatt tcttccgcaa gcgctacaag ctggaaggca cgccgatccg
     3421 catcgaattc cgcgaaggcg agaacccgta cgccggcagg cgcaacgtat tgaccgaagc
     3481 ccagcaacgc aagcgcaggc ggatggtccg gcacgtccgg agccggcgat gagcgggata
     3541 ggcaccctcg gcgtgatcct cgccggcggc gccggcagcc gcgtcggcgg cgcggacaag
     3601 gggctgctgg cggtgcatgg cgcaccggtg gtcgaacacg tggccgcagc gctgcgcccg
     3661 cagtgcgacg acatgctgat cgtcgccaat cgcaaccggc cggactacgc gcgggtcgcg
     3721 cgcgtcgtcg gcgacgagat cccgggccat gcgggtccgc tggccggcat cgccgcggcg
     3781 ttggcgctcg tggtcgagtc cggagagaac ccattcgagg attgccgctg gcttttgacc
     3841 gtgccggtcg attgccccga tttcccgggc gatgttggcg atcggctgcg cggggcgttc
     3901 gacggcgacg cacgattggc ctgcgcatac gcacgcgatg aacgaagact gcaacccttg
     3961 ttcgcgctgt actcgctcgc gagtggcgga gccttgctgg aatcggcgcg cgccgcggca
     4021 tcgctgcacg gatccgtctt gcgctggcac atggggctgg cagcgcgcgc ggtggaattc
     4081 cgcgggcagg cggcggcatt ccacaacttg aacgcacccg aggactttcg cgagtacgaa
     4141 cggagccatg ccgcaatctg attatccgac gcggctgagc ctgggggatg cgctcgcgat
     4201 cgtggccacg cgcgccgcgc agcaccgcct gccggtcgag accgtcgacc tggcgacggc
     4261 gcacgggcgc gtgctggcca aggacatcca cgcaccgcat ccgctaccgc cgttcgacaa
     4321 ttccgcgatg gacggctttg ccgtgcgcgg agaagacctg cccgcaaagg gcgagcgcca
     4381 gttccgcctc acgggggagg tgttcgcggg cgcgaccgcc gcgccagcgt tggaagcggg
     4441 cgagtgcatc cgggtcacca ccggcgcgcc gatgccgccc ggcgccgaca ccgtggtgat
     4501 caaggagaac gccgtcgtcg acgtcggcga agtcacgttg aaggccggag aaaaggcggg
     4561 cgccaatgtc cgcagcgcgg gggaagacta cgcccgaggc gatgtcgcgc tgatggcggg
     4621 tacctcgctg cacgctccac aggtcgccgt gctggcggcg ctcggcgtgg cggaggtttc
     4681 ggttcggcgc cagccgcgga tcgcggtgat tgccaccggc aacgaactgg tgcccgccgg
     4741 gcaaccgctc ggcttcgggc agatccacga gtccaacgcc gtgatgctgg cggcactcgg
     4801 gcgcgaggcc ggcgcgccca tcgccacgca ggaagtcgtg cgcgacgatc ccggggcgct
     4861 gcgcggtgcg ctcctccgag ccgcggccga cgccgacatc gtcgtgacca gcggcggcgt
     4921 gtcggccggc gaagccgacc acctgccgcg cgtgctgcag gccgtcggcg aaatccattt
     4981 ccacaaggtg cggctcaagc cgggcatgcc gacgctgttc gggcaaatcg gcgccagcct
     5041 ttatttcggg ctgcccggca acccggtcgc gtcggcggtg acgttccggg tgttcgtcgg
     5101 gttcgcgttg cggtcgatgc tcggcatcac gaaagtgtcc gaacctcgac gggcccggct
     5161 ggacgcgccg ctgcacaagc gccatgcgcg cgcggaattc gcgcgctgca gcctgcgcag
     5221 cgacgagcac ggcgccctgt gggcgacgct gcatgccagg caaggctcgg gcatgctgcg
     5281 cggcctcgcg gaaaccgagg cgctcgcctt gctgccggag ggcacgcgcg aattcgccga
     5341 aggcgacgtg gtcatgttgt ggccggaaca cttttggcac ggatgaacgc ggatctgcat
     5401 ccacgccgct gggcggatac cgtccgccgg tttggtagat cgcatggcac gcggcggaca
     5461 acgcgcgccc tacgttttgc tccggaaaat ccttgacacg gatcgacgcg gatggaagcg
     5521 gatcattgtt ctgctttatc cgtggtcatc tgctgttatc cgtgtccaat ggcttttgca
     5581 ccataatgcg ggcatgaccg tccgcgaaat ttccccgcag gaggccctcc agcgccagca
     5641 ggcaggcgcg ctgctgctgg acgtgcgcga gacctacgaa cacgcgaccg gcatggccga
     5701 aggcgcgatc gcgctgccgc tcggcgaggt gctgcaacgg atcggcgacg tcgctcccga
     5761 ttcgacgcgc gaaatcctcg cgatctgtgc gcacggacaa cgctcgttgc gcgcggcgca
     5821 ggcgctgcag caacgcgggt acgccgatgt cgtttcggtc gccggcggca ccgtgcgctg
     5881 gatcgaggaa ggcctgccga ccagcaagcc cggcggtgac gaggatttct tcgatcgcta
     5941 ctcgcgccac ctgcgcctgc cggaagtcgg cgaggccggg cagcgcaaac tggaatccgc
     6001 gcgggtggtg cttctcgggg ccggcgggct gggctcgccg gcggcgtact accttgccgc
     6061 cgcgggcgtc ggcacgctgg tgatcgccga cgatgacgtg gtcgagcgca gcaacctgca
     6121 acggcagatc ctccacaccg acgatcgcat cggcgtgtcc aagctccaat ccgccgcgca
     6181 cgccctgcac gggctcaacc cgcgggtgga catcgagggc ctgcccgagc gcgtcacctc
     6241 cgccaacgtc gatgccttgc tcgaaggtgc cgacgtggtg ctggacgggg ccgacaactt
     6301 cccggtgcgc tacctcctca atgacgcctg cgtgaagctc gcaattccgc tcgtgtacgg
     6361 cgcggtgcag cgtttccagg ggcaggtcgg cgtattcgac gcggggcgcc gtcgcggcgt
     6421 tgcgccgtgc taccgctgcc tctttccgca accacctgca ccggaagacg cgccgaactg
     6481 ttcggaagcc ggcgtgctgg gcgtgctgcc cggcgtcgtc gggatgctgc aggcgaccga
     6541 ggtgatcaag ctgatcctgg gtatcggcga accgttggtg gggcgcgtcc tgatgttcga
     6601 tgccctggcg atgcgattcc acgaaacacg cctcgcgccg gatcccgaat gcccggtgtg
     6661 tgcacccggc aaaccgtttc ccgggtacat cgattacgag cggttctgca cggggtagcg
     6721 cgggtgacgc ccgcaacggg tgcattggcg gccggcgtcc gccctgcggg attcgcggaa
     6781 tccaggtgcc aatcgaagga acgtggcgcg gggactgcca gcccaatggt ttgctgccgg
     6841 tccgctccgc tcaacccttt ttcggcgcag cctcgaatgc cgccaactgc tcgggtgtcg
     6901 cgtccttctg gtatttcgcc ttccattcgg cgaacggcat gccgtagatc gcttcgcgtg
     6961 cctggtcctt cgtcatttcc gagccgcgct cggcggcggc tgcgcggtac cagtcggcca
     7021 ggcagttgcg gcagaacccc gcgaggatca tcaggtcgat gttctggacg tccagcctgt
     7081 cctggttgag atggcgcagc agccggcgga aggcggcggc ttcgagttgg atccggtcgg
     7141 gtgatgacat gctggcgttt tgcgcgaacc gcggaatcgg cgagaatgca cgcttactgt
     7201 aacgcatggg gtccgcctcg gcatgcctga aaccgcccac aacgcacgca tcctcgatgg
     7261 ccgcaggatc gccgacgagg tgctcgagca ggtcgccacc ggcgtcgcgg cccggctcgc
     7321 cgccggccgg tccgcgcccg ggctggcggt ggtgctggtc ggcgccgatc cggcgtcggc
     7381 ggtctacgtg cgcaacaaac gcaaggcctg caagcgcgcc ggattccgtt cgttcgacta
     7441 cgaccttccc gcggagacgc cggaggccga actgttcgcg ctggtggaca ggctcaacgc
     7501 cgacccggag gtccacggga tcctcgtgca gttgccgctg ccgccgcaca tcgacgccag
     7561 cgcgctgatc gaccggatcg atcctcgcaa ggacgtcgac gggttcagtg ccatcaacct
     7621 cgggcatttg gcgctgcggc gtttcggctt gcggccgtgc acgcccaagg gcgtgatgac
     7681 actgctcggc cgcaccgacc ggcccgtacg cggccagcac gccgtggtcg tcggcgtctc
     7741 caaccacgtc gggcggccgc tggtgctcga gctcctgatc gccggctgca cgacgacctg
     7801 ctgccacaag ttcacgcagg gcctcgacca gcatgtcgcg cgtggcgaca tcgtgatcgc
     7861 cgcggtcggc aagccgggca tcgtcaaggg cgagtggatc aagccggggg cggtggtgat
     7921 cgacgtcggc atcaaccgga tgcaggacgg gcaccttgtc ggcgacgtcg aattcgagga
     7981 agccgccaag cgcgcatcgt ggatcacccc cgtccccggc ggcgtcggac cgatgaccgt
     8041 cgcgacgctg atggagaaca cgctggaggc ggcgcaagcg ctcgacggcc cgtaggacag
     8101 cgagaagggg tcagggagaa ggggtcagag tgcactttcg gcagagtggg tgacctgtcc
     8161 gaagccgcga ttccgcatgc cgaaagtgca ctctgacccc ggttttccgg gcaagctcct
     8221 acaacgccct gcgacaacgg cgtataattg cggattgcat tgagggatag catccaatgc
     8281 gcatcctcgc cgaggccctg acctacgacg acgtctatct cgtccccgcc cattcctccg
     8341 tcgtgccgcg cgacgtcgac acgtcgaccg acctgacccg cgcgatccgc ctcaacatcc
     8401 cgctgatgtc ctcggccatg gacaccgtga ccgaagcgcg cctcgcgatc acgatggcgc
     8461 agacgggcgg catcggcatc atccacaaga acatgaccgt agagcgccag gccgcggaag
     8521 tgcggctggt caagaagttc gaggccggcg tgatccgcga cccgctgacg gtcgggccga
     8581 cgacctcgat cggcgaggtg atgcacatca cccggtcgca caacatctcc ggcgtgccgg
     8641 tggtggaaga gggcaacaag ctggtcggca tcgtcaccag ccgcgacctc cgcttcgaga
     8701 agaagccgga tgatccggtc aagaacatca tgacccgcaa ggacaagctg gtcacggtgc
     8761 aggaaggcgc gggcgaggac gaaatcgtcg gcctgctgca caagcaccgc atcgagaagg
     8821 tcctggtggt caacgatgcg ttcgagctgc gcgggctgat taccgtcaag gacatccaga
     8881 aggcccgcga caacccgcat tcggccaagg acgaccacga gcgcctgcgg gtcggtgccg
     8941 cggtaggcgt gggcggcgaa accgaacggc gggtcgcggc gctcgccgag gccggcgtgg
     9001 acgtgatcgt ggtcgatacc gcgcacgggc attcccaggg cgtgatcgaa cgcgtcgcct
     9061 ggatccgcaa gaacttccct ggcctgcaga tgatcgccgg caatatcgtg acgggcgatg
     9121 ccgccaaggc gctgcgtgac gcgggcgtgg atgcggtcaa ggtcggggtc gggccgggct
     9181 cgatctgcac gacccgcatc gtcgcgggcg tgggcgtgcc gcagatcacc gcgatttcga
     9241 tggttgccga tgcgctgaag ggcgacattc cgttgattgc cgacggcggc gtgcgctact
     9301 ccggcgatgt cgccaaggcg atcgtcgcgg gcgcgtcgtg cgtgatgctc ggctcgatgt
     9361 tcgccggcac cgaggaagca ccgggcgaaa tcgagctgta ccaggggcgc tcgtacaaga
     9421 gttaccgcgg catgggctcg ctcggcgcga tgcagagcgg atcgtccgac cgctacttcc
     9481 aggaacaggc cgcggccgac aagctggtgc ccgaaggcat cgagggcagg gtgccttacc
     9541 gcggcccact gcgcgcgatc gtgcatcaac tggttggcgg gctgcgggcc tcgatgggct
     9601 actgcggctg cgcgaacatc gagcagatgc gcaccatgcc acagttcgta cgcgtcaccg
     9661 gcgccggtac ccgcgaggcg cacgtccacg acgtgcagat caccaaggaa gcgccgaatt
     9721 acaggttgga ctgatgaggc agggaccggg accggggacc ggggaccagc caagcgtgca
     9781 gcaccgcctt cgcgtgaagc ggcgagacgc caggacgcca caattctctc ttgcgcatcg
     9841 gggaatccgg tccccggtcc ccttacccgg tccccatgtc aaacatccat accgacaaga
     9901 tcctcatcct cgatttcggt tcgcagtaca cgcaactcat cgcgcgacgc atccgcgaga
     9961 tcggggtgta ctgcgagatc tgggcctggg accacgatcc ctccgaaatc gcgaagttcg
    10021 cgccgaaagg ggtgatcctt tccggcgggc cggactcggt ggtggaagcc ggttcaccgc
    10081 gggcaccagg cgacgttttc gcgctggatg tgccgatcct gggcatctgc tacggcatgc
    10141 aggcgatggc ggtgcagctc ggcggcaacg tggagggcgg ccacgcccgc gaatacggac
    10201 atgcgatcat cgccaccgaa agctgcgcct tgttcgaagg cacgccggcc cggcaggacg
    10261 tgtggatgtc ccatggcgat cgcgtgacgg cgctgccgac cggcttcgcg ttggccgcct
    10321 ccacggaatc cgtgccgatc ggagcgatgg ccgacgaggc gcatcgctgg tacggcatcc
    10381 agttccatcc cgaggtcacc cacaccgaac acggcatggc catcctgcgc cggttcgtgg
    10441 tcgacatctg cggttgcgcg acgctctgga cgcccgcccg gatcatcgac gatgcgatcg
    10501 cgcaggtgcg cggcacggtc ggcgacgaca gggtgctgct gggcctttcg ggcggcgtcg
    10561 attcctcggt ggtcgcggcg ctgctggaaa aggcaatcgg cgatcaattg acctgcgtgt
    10621 tcgtcgacac cggcctgctg cgcgcgggcg agggcgacca ggtgatggat acgatggcgc
    10681 gccacatggg cgtgcacgtg atccgggtcg atgcctcgga tcgtttcttc gccgcgctgg
    10741 aaggcatcgc cgatcccgaa gccaagcgca agatcatcgg caggctgttc gtcgaggtgt
    10801 tcgacgaaga gtcgcacaag ctccagggcg tgaagtggct cgcgcagggc acgatctatc
    10861 cggacgtgat cgagtcggcc ggcggcaaga ccggcaaggc gcacgtgatc aagagccacc
    10921 acaacgtggg cggcctgccc gagaagatgc acctggaatt gctggaaccg ctgcgcgagc
    10981 tgttcaagga cgaggtgcga cggatcggcg tggaactcgg cctgccccgc gagatggtct
    11041 atcgacaccc gttccccggc cccggcctcg gtgtgcggat cctgggcgaa gtaaggcgcg
    11101 agtacgccga catcctgaaa gccgccgacc acattttcat cgaagagctc cgcgccgcgg
    11161 atttgtacga caaggtcagc caggccttcg cggtgttcct gccggtgagg tctgtcgggg
    11221 tcgtcggtga cggccgcgcc tacgaatggg tgatcgccct gcgcgccgtg gagaccatcg
    11281 acttcatgac tgcgcactgg gcccatctgc cctacgagtt cttgggtcgt gtctccacta
    11341 gaatcatcaa tgagttgcgt ggtatttctc gcgtcgtgta tgacatcagc gggaagccgc
    11401 ccgccaccat cgagtgggaa tgattcccgg tcggccggca ggggcgagac cgtgacgcgc
    11461 gcgaccgcgc aatgctaatc tgcatcgctg cccgcgctca tggccctgcg cggttgcata
    11521 gagggaatct gcacatgagc acctccaagg acgagcccga ggctgaactg ccgcggatct
    11581 ccaccggcag caacggtctg gacctgatac tcggcggcgg cttcgacccg cagcgcatgt
    11641 acctgtacga ggggcagccc ggggcgggca agaccacgct ggccctgcag ttcctgctgg
    11701 agggcgtgcg ccggggggaa cgcaccctct acatcaccct ttcggaaacc gagcgcgaac
    11761 tggaactcgt ggcccagcgc cacgggtggt ccctggccgg cgtcgatgtt ttcgaactcg
    11821 ttccatccga aacgacgctg gatccgagcc aggagttgac gatcctgcag ccctccgaga
    11881 tggaactcag cgagacgagc caactgatct tcgacaaggt cgaagagctc aatcccagcc
    11941 gaatcgtgat cgacagcctg tcggagcttc ggctgctggc acagagcgcc ttgcgctacc
    12001 gccgacaggt cctggccctg aagcactttt tctccagccg caagtccacc gtgatcctgc
    12061 tcgatgacct cacggcgcag cagaaggacc tccagctcca ttcgatcgcg cacggggtgg
    12121 tgctcctgga gcagttggcg atcgactacg gctcggaacg ccggcggctg agggtggtga
    12181 agatgcgggg catcccgttc gcgggcggct tccacgattt caccatcgag cacgggggcc
    12241 tgcgggttta tccccgcctc gtcgctgcag agcaccacaa ggatttcgct ggcgcttacg
    12301 tgcccagcgg caaccgcgaa ctcgacatgc tgttgggtgg cggactggag cgaggcacca
    12361 acgccctctt gatcggagcc gcgggcgtcg ggaaatcatc gattgcggtc acctatgcca
    12421 tcgcggcagc gcagcgtggc gaacatacga tgatcttcgc gttcgacgaa gggcggggca
    12481 cgatcaatgc ccgcgcgaag tcgctcgggc tgccgttcga agaggccatc gagagcggca
    12541 tgataaagct gcagcagatc gatccggcgg agctgtcgcc cggagagttc gcgcagttgg
    12601 tccgccaatc cgtggaaacg gaagaagcgc ggatcgtgat catcgacagc ctcaacgggt
    12661 acctcaattc gatgccggac gagcgctttc tcgtcctgca aatgcacgag ctgctgagtt
    12721 acctgagcca gctcggcgtt ctttccatac tggtgctggc acagcacggg ctggtcggag
    12781 cgatgcaaac gcccgtggac atcagttacc tgagcgacgc cgtgctgatg ctgcggtact
    12841 tcgagtacga cggcaccgtt cgccgggccc tgtcggtggt gaaaaaacgc agtgggcacc
    12901 acgaacacac catccgcgag tttcgattga gccaccaagg ggtcaggctg gggccgcccc
    12961 tgaaagggtt caacgggatc ctgtcgggca cgccgatcta caccggcgac aggagcccgt
    13021 tgctggcgga tgaagacagc cgggccggct gacctcatgg acgcaagggt cctcatcctc
    13081 gcgccggtcg gccgtgatgc gggcgcctgc gcccgactgg tcgcgcaggc cgggctggtc
    13141 ccgcaggcgt gcagcgacat ggccgaattg gtggcacagc tcgacctcgg cgccgacatg
    13201 gtgctgctta cggaggaggc gctctacggc accgcgctgg atccgctggc cgggtgggtg
    13261 gcctcacaac cgccctggtc ggacctgccg ttcatcgtga tgaccagcca caacgaaggt
    13321 ccgcgcttcg cccggtttcg cagggacctg gtccgcaagc tccgcaacgt ttccttcctg
    13381 gaacgtccga tgcaggcgat ttcgttgcag gcggccgtgt tgtcggcggc acgcggacgc
    13441 gcccgccagt acgaggcgcg tgcttatctg gaagcacagc gcaacgccgc cgaggatctt
    13501 gaacgcctgg tcgcccagcg caccgaagcc ctgcaagccg ccaacgagca gttgcgcgag
    13561 gaaagcaggc ggcgcgaacg cgcgcaggcg gcgctcatgc aggctcagaa actggagacg
    13621 atggggcaac tcgtcggcgg tgtcgcgcat gacttcaaca acctgctcat ggcggtgatc
    13681 ggaaatctcg aattgcttgc caagcgaatc gatcatgatc cccgccaggc aaggctgctg
    13741 cgtggtgcca tggagggcgc gcgacgcggc gccaccctga cccagcggct gctcgccttc
    13801 gcccgcaagc aggagctgca gttccgggcc accgatgtgc tggcgctcgt caaggacatg
    13861 cgcgacctga tcgcgcggtc ggtcgggcct ttgatcgacg tgcagatccg ggcaagcgaa
    13921 ccgctgcctc ccgtcgtcgg tgatccgaac cagttggaaa tggcgctcct gaaccttgcc
    13981 gtcaacgcgc gggacgcgat gccatccggc ggggttctca cgatcagcct ggagcaatgc
    14041 gttgcgaccg cggagcagtc ggaattgaag ccgggtgtgt acgtgaagtt gtcggtggcg
    14101 gacaccggcg agggcatgga cgcggctacg ctcgcgcgag ccgtggaacc ttttttctcg
    14161 acgaagggtg tcggcaaggg aaccgggctt ggtttgtcca tggtgcatgg actggctgag
    14221 cagtcgggtg gaaccttccg gctggaaagc cgccagggcg tcggcaccaa ggccagcctt
    14281 tggctgccgc tcgcgcacag cgagctggaa gtcgctgcgc cgcccgagcg gctcgggacg
    14341 acggcagcgg cggccatcat cgtgctggtg gacgacgatg cgctgattgc gaccagcacg
    14401 gccgccttgc tggaggatct cgggcaccag gtcaccgaat tccggtccgg acaggaagcg
    14461 cttgctgcca tccgcggcgg gcttcgcccg gacatcgtga tcacggatca cgcgatgccg
    14521 gggatgaccg gggtggagct cgcgattgcg ctgcgcgagc tcgagccgga gctgcccatc
    14581 ctgctggcga ccggttacgc cgagctcgaa ggagccccgc gcatcgacct tccccgcatc
    14641 gccaagcctt acacccagca gcaactctcg gtcgaaatcg aacgtctgct gtcccagcgc
    14701 gttcattcaa cctgacttcg agcacgggtg tccttgtccg ggagctcgcg ctcgcgtcga
    14761 cgcttccgcc tcgtgcaggt aggtcgtgaa accgtccctt gccggagcaa gcgcattgag
    14821 cagcatcacc gcgcttacct ggccccggtg gcccgtgccg tggttgatca cgtgcacgag
    14881 catctcctcg cgcgacatgc gtgccggtgc accgtcggtg aacgcgaaat cgattcgttc
    14941 catcagttgg tccgcatcga gcgtcggcac gtagtcgacg tactcgcggt cgctttccct
    15001 gatatcggcg gacaaaccct cgagcgtcgg cgtttcggcg gggttgactg ccgtgtaccc
    15061 gtggggcttt ccccgcaggt gtgccgcgaa gatccggtcg acgatgtagc tgtggcccag
    15121 cgccttgatc gccagctcgg taacgggaga tttcccgtcg agccgggcca gcgcgtccaa
    15181 cagcgcatcg tcggcacacg ccttgtatcg aaacagccgc tgcagtgtgt catgcatgtc
    15241 ctggtcctcc tcgggtcttg cgcgccgcga gctgcaacgc gttgcattcg tgtgccatgc
    15301 aagccagaat atgagcgatc gctcgttttg tgtactcgca ttgacggcct gtctttcatg
    15361 gcgcgcaaac cacccacgaa acccaggaaa acggccagcc aggcccgatc gcgcgcgacc
    15421 gtcgaggcgc tgctcggagc gactgctcgc attctcgtca gggagggctt cgacaaggcc
    15481 agtaccaacc gcatcgccga agtggccggc gtgagcgtcg gctcgctgta ccagtatttc
    15541 ccgggcaagg aagcgctcgt tgccgccgtg atggaacgcc accagcatga gatcatgcag
    15601 gaggtgcggg acggactggc gaagcttttt gcactgccgg tggagcaggc gatgcgcgaa
    15661 ctcgtcgccc tcgcggtgaa ggcgcatcgc gtcgatcccg ccttgcatcg cgtgctcacg
    15721 gagcagatcc cgcgggtagg caagctcgaa gaacgggaag ccttcgatcg cgggaacttc
    15781 accctgttcc ggacatacct cgaacgcaat cgcgacgaac tccacgtccg tgacctggat
    15841 ctcgcaacgt tcgtgtgcgt gacgtcgatc gaggccctga cccacaacgc ggtgctgcat
    15901 taccccgcgg cgcttccggc ccaggcgatg gagtcgctcg tcgaggaggg ggcgcggctc
    15961 gtaacgggat acctcaaggc gtagccggta ggccggcacc ctcggggggg tgccccaagg
    16021 ccctcaatgc cgacgaacga ccctgatcct gctgctgctg ccgcccccgc acaggaattg
    16081 tccgttgccg tccgtttcca gcccggaaac ggtggtccct tccggcatgt cgatctgctc
    16141 caggacttcc ccactcacgg gatcgatccg gcgcaggtcg ctctcgtctt cttcccaggt
    16201 gccgtgccag aggtcgccgt ccgcccaggt gacaccagtg acgaagcgcc tgcactcgat
    16261 ggtgcgcagg atctttcccg tttccggatc gatctggtgg attttccgcg cgcggtattc
    16321 gccgacccac aaggttcctt cggcccatgc cagacccgag tttccgccgt caccgggcgc
    16381 cggaatcgtg gacagcacgc gtccgttcgc gggatcgatc ttctggatgc gatcctcggc
    16441 gatctggaac agatgcttgc cgtcgaacgc cgtgcccgcg tgtgccgcga catcgatcga
    16501 gcgcacgggc cttccgctcg cgggatccac ggcgttcaac ttgtcgccgg acgcgaacca
    16561 gacgcgctct ccgtcccagg ccacgccgtg cacccgctcg gcgtcgggga acgggccgta
    16621 ttcgcgaagg atttcagcgg atgaccgttt catgattgcg ccctgatcgc tcgtgacggc
    16681 gccatcctaa tccccgggga gcggagcggg gagcaacaat gctgtcgtga atcccggcag
    16741 ggatggcgtg gcccagcgac gtgcacggcc gcgtcccacg ggctggatct tggcgtccgc
    16801 cgccagcgca tcgagcgccc gttgcaggtg gcgctggctc atccccagcg ccatggccaa
    16861 cgccgagctc gaccacggtt ggccatccgc aagcagcgcc aggaccgccg catgtttttc
    16921 ttccgtcggg cgcgccagca ccgcgacctc gcgcgcgcgg gccggcacca ctgcgaatcc
    16981 gcgccgagtc gcgcggatgt gggcgaagga tcgaagcgcg gcccttagtc gaccgatctc
    17041 gacccgcagc cgggcgcgat gtgtctcgtc cgcgaatttc gtatggaatg cggtggtgat
    17101 cagtacgtgc cgcgacgcgt cgtgcggcca ggcttcggcc agtgcctgcg caagcgcaaa
    17161 cagcaccggc ctggatgcga gcgacaccgt ggctcgtcca tcgcggacgg ttcggcggca
    17221 agcgtccacg accaatgcct ccgacgcgaa cagtgcctcg acctcatcca gccgcaggaa
    17281 cctttcccca ccgcggccga tcagtcgggc ggcgggcgca tccagcatgc gctgtgcgta
    17341 ttccacttcg gcaagcagcg ccggaacgcc ggccccgcgc gccgcccgtc cggcgcgcgc
    17401 gagcgcggca cgcgcggtcc ccgcccggac gcgtcgcacc gcgatcccgg ccgcggtcaa
    17461 ttcgtggaca gcccgcaacg ccggtggcaa tggcgcggat tcgaatgcgg caagcgcacc
    17521 ttccgcctgg tcgagctggc cgatcaacag cagccggcgg atcgcgagat agcgcgcatg
    17581 tgccgcattc acccggtcgc cgtgcgcttc cagcacgtcc tgcgcagcct cgagccttct
    17641 ttcgggccaa ccgagttcac gggaggcaag ggcgacctcg gcctcggcga ccacgcaccg
    17701 cgcgcgtgcc acggtttccc tgcgcccgaa agcccgtgtc gcacggcgca gcagcgcttt
    17761 cgcgcgttcg aaatcgccga gctgggcgag cgcgatgcca cgcaatgcca aggcaggcgc
    17821 atcctcgcgc agcgcaacac gcttcagtgc acccagcgga tcgcctgccg ccagtgcacg
    17881 cgccgcggcc gtgatcagcg aatccatggc acccgcatca tcaatcgcgc cacatttgtc
    17941 actcccgctc ccgcgcgccg ggcgcctaat ctacctcacg acgatcaacc aagccgccgt
    18001 tcgcgcagct ggcgagcggc cggaaacccg gaggagaaga cgatgaccac gcacaagacc
    18061 ggcacgcgcg acgagtggct ggaagagcgg ctccgcctgc tcgacgcgga aaaggaactc
    18121 acccgccgca gtgacgaact ggcgcaacgg cggcaggagc tgccgtgggt gcgcatcgag
    18181 aagccatacc acttcgagac cgatgacggc gaggccagcc tggcagatct gttccgcggg
    18241 cgttcgcagc tgctggtcta ccacttcatg ttcgggccgg actacaccgc cggctgccct
    18301 tcatgttcca tgatcgcaga cggcttcaac gggtttaccg ggcatctcgc aaaccacgac
    18361 gtgatgcttt gggccgtttc gcgtgcgcca ctgccgaaac tgaaagccta tgaacggcgg
    18421 atgggctgga cctttccctg ggcgtcctcg ttcggcagcg atttcaattt cgacttcgcg
    18481 gtcggcatta ccgagatgca gcaacgcgaa ggcggtgcgg aatacaacta tcgccgtgag
    18541 gccgcgactt catccggagg cacgccagac ggcccgagca cgaaattcgg cagtgaaagt
    18601 cccgcgtgcg cttcgcacga tcgtgcggga cggatccttc gacgtacctg cgtgaacgtc
    18661 cgggcatgag cgcgttcgtg ctggacaatg gcgtggtcta ccacacctat tccagctact
    18721 cgcgcggact ggacggcctt tggggcacat accagtggct cgatcgtgcg ccgcgcggcc
    18781 gcaacgagga aggcggcatc tggtggcggc ggcacgacga gtacggacgg tcgtgatgcg
    18841 atgcgccgat acccgcggcg ccgtggcgtg gctgcacctc gcctccaccc cgaccttcgt
    18901 ggcgatggcg gtggccaacg cggcgatgcc gggcgcgggg atggacatgc tttgcccggc
    18961 gttgaacggc gtcacgccgc tccctggcag catggcgatg atgtacctgc tgatgggggc
    19021 cttccacgcg ggaccgtggc tgcgaatgtt catcgtgcgg cgcaagcggc tccgccggtc
    19081 cggctttccc ggtgggcgcg aacgcggtcc ggcccgcatg aatgcttata tatgcccagc
    19141 cgcgacgaag gtgcaggcca cggccgacgc gcgtaatcca gcgcctttca tgcaggaaac
    19201 gaaggcactg gatgccgctc cgtcatccga atgacgaaca aagcgtcacg gcctgcgcgc
    19261 gcccaggtcg gctcaagcca ccttgtcgta tttgtccacc gccccagcca tctcggccag
    19321 gcaccaccag catcggatcg gctatgccct gcaggtccga aagcctgcgc gtcgtcttga
    19381 ttggggagtt cgtagtcggg aaaggcctgg ccaaccgcga tcttgccgtt catggtgact
    19441 ccggtttcgt tccgcggcgc aaggcgcttc gagagcactt tgcccgcttg tagacttgag
    19501 cgacacgcta gtcctgtgcg catgactgac cacccaacgc ccccgctgcg cgaagacgac
    19561 gttgcatgga tgcgccgtgc gctggaactg gcgatccacg cgcgcgacgc ggacggcgaa
    19621 gtgccggtgg gcgcggtgct ggtgctggac ggcgaaatcg tcggcgaagg ctggaaccgc
    19681 aacatcaccc tgaacgatcc cagcgcccac gcggaaatcg gcgcgctgcg agatgccggc
    19741 aagcgcctcg gcaaccaccg cattccggga tccacgctgt acgtgacgct cgagccctgc
    19801 gcgatgtgcg cgatggcatt ggtgcacgca cgggtggcgc gtgtcgtcta cggtgcggcc
    19861 gatccgaaga ccggcgccgc gggcagcgtg ttcgacacgc tggtctcgga gcgccacaat
    19921 catcgcgtcg cggtcgaggg tggactgctg gccgaagaat cggcggaact gctgagaggt
    19981 tttttccgcg ccaagcggtg aacggacatt cattgcacgc ttcgcgggcg acgaattctt
    20041 tgccggaaat attttcgacg ctggcctcgt cgacggcgca gtcgacgccg cgcagcgcgc
    20101 gtagcgcgga cttgcgcgtg gcacatggca tgcatgcccc ggggtcttcg gcacgaggag
    20161 caaagcccat ggcacgctac cgcgaaagat atggacagga agaccacggc gtgggcgacg
    20221 ccgcgcgccg ccatgcggag gcggattatc gcgatcagtt cggtaccggc ggccgcacgc
    20281 aaaaccttcc ggacgactac ggcaccgggc gcgatccgca ggcgttcgag cgccgctatc
    20341 gcgatgagcc gtattacgag gagcggtacg gcgaaggcga atcacgcttc ccgggcggcg
    20401 gctggagcag ccaccatcgc ggcggcggcc ggcccggctg gcgcggcgac gcgggattcg
    20461 gccgcaccta cagcggcaac tacgaaagcc tcgggcgcag ggaaccactg cgctacggcg
    20521 aaggcggcgg ccaggcgatg gcgcgcggct accgcggccg cggccccagg aactacacgc
    20581 gttccgacga gcgcatccag gaagacatca gcgagcgcct ctgggacgcc gatgacgtgg
    20641 acgccagcga cgtgacggtc gaggtgaaga acggcgaggt caccctgcgc ggcaacgtcg
    20701 agcggcgcag cgtcaagcac cgcatcgagg acatcgcgga cgggtgcagc ggcgtcaagg
    20761 acatccacaa cgagatccgc gtgcagccgc gcaacgagtg gccgtacgac aagggcgtcg
    20821 cggccggctc gatcgacgac gcctcgcgcc gctccggcaa cggcgagcgc aacgaaggca
    20881 tggtccagtc cgcgatcgag gagatgcctc cgcgttcgca ttgaacgcgc tccgcacctc
    20941 ggccggggtc gcgcggggac ggcgttgcag gacggacacc ttccggcgat cggttcgagg
    21001 aagttggacg gcggaaagtg cccgccctac gcgtctaccg gcgcgacgct tgcgccgagc
    21061 gcctcgctga tgtggtccag cgcacgctgg ttgcgcttgg gcgtgagcga tgcggtcgca
    21121 ttggccggta cccacaggtg gtagtcgcgg atgtgggcgt ccatcgcggt cgtcatcacg
    21181 cattggtcgc cggccacccc ggtcagcacc agccgcgaaa ccttcaggta ttcgagcagc
//
LOCUS       DASSNH010000252         6108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_772145, whole genome shotgun sequence.
ACCESSION   DASSNH010000252 DASSNH010000000
VERSION     DASSNH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772145"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /gene="thrS"
                     /locus_tag="VFK00_09375"
     CDS             <1..1842
                     /gene="thrS"
                     /locus_tag="VFK00_09375"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7593201.1"
                     /translation="VAASIGAGLAKAALAGKVDGKLVDLSRTIDHDARVEIVTEKSPE
                     ALDVIRHSTAHLLAQAVQRLYPGTQVTIGPVIEDGFYYDFAPRGEPFKPEDLPAIEEE
                     MRKIAKESLPLSRSVKSRDEAVQFFRGLGEDYKAEIIESIPANEDLSLYSQGEFTDLC
                     RGPHVPDTGKLKSFKLTKTAGAYWRGDSDNAMLSRIYGTAWLNDKDLKAYLTRIEEAE
                     KRDHRKLGKQLDLFHMQEEAPGMAFWHAKGWTLWQQVEQYMRRVYRDSGYQEVRCPSI
                     LDVSLWKKSGHWDNYKDNMFFTESEKRTYAVKPMNCPGHVQIYNDGLHSYRDLPIRYG
                     EFGSCTRNEPSGALHGLLRVRSFTQDDGHIFCTEDQIEQEVVDFHRQALKVYGDFGFT
                     NVELMLALRPESRIGDDAVWDKSEDALRAALRRAGAEWKELPGEGAFYGPKIEYHLRD
                     AIGRDWQLGTMQVDFSMPGRLGAEYVGEDSQRHTPVMLHRAIVGSMERFLGILIEHHA
                     GNFPTWLAPVQAVVMNITDSQAEYAERAAANLNEAGFRVEADLRNEKIGYKIREHTLQ
                     KVPWLLVVGDREKERGTVSVRTRSGEDLGSMSLPGFIQRLEAEPRPR"
     gene            1891..2412
                     /gene="infC"
                     /locus_tag="VFK00_09380"
     CDS             1891..2412
                     /gene="infC"
                     /locus_tag="VFK00_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7593202.1"
                     /translation="MATENKGNRRNQDIRVPRVRVIDADGEQVGILDRDAALELAGEA
                     GLDLVEIQPTADPPVCRIMDFGKFKFEQQKKAAAQRKKSKQVETKELKFRPTTDVGDY
                     NIKLRNMVRFLEEGDKVKVNIRFRGREMRHQELGIELAQRIQKDISETGTVDQFPRME
                     GRQMTMMISPKKK"
     gene            2833..3030
                     /gene="rpmI"
                     /locus_tag="VFK00_09385"
     CDS             2833..3030
                     /gene="rpmI"
                     /locus_tag="VFK00_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7593203.1"
                     /translation="MPKIKTNRAAAKRFRKTATGKFKAGHAFKSHILTKKSTKRKRGL
                     RAPNHVHESDTKRVARMLPYV"
     gene            3046..3405
                     /gene="rplT"
                     /locus_tag="VFK00_09390"
     CDS             3046..3405
                     /gene="rplT"
                     /locus_tag="VFK00_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7593204.1"
                     /translation="MARVKRGVTARARHKKTLKAAKGYYNARRKVYRVAKQAVTKAQQ
                     YAYIGRKQKKRQFRALWIVRINAAARQFGLSYSRLIAGLDKAGITVDRKVLADIAVHD
                     IAAFGAIAEKAKASLAA"
     gene            3496..4491
                     /gene="pheS"
                     /locus_tag="VFK00_09395"
     CDS             3496..4491
                     /gene="pheS"
                     /locus_tag="VFK00_09395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7593205.1"
                     /translation="MHTLAQRTDEALAAIAAAATLDALEAQRVALLGRNGAITAELKA
                     LGKLPPDERKMRGAEVNAARQALDAAITARRASLEQAALDQRLASERVDVTQPGRDGG
                     RGSIHPLTRTRERIEAIFAQLGYSVAEGPEIEDDWHNFEALNFPAHHPARAMHDTFYF
                     DDGRLLRTHTSPVQIRAMRGKQPPVRIIAPGKVYRSDSDQTHSPMFHQVEGLLVDEAA
                     TFADLKGTLEAFVAAFFGKALEMRFRPSYFPFTEPSAEVDIRWQREDGEARWLEVLGC
                     GMVHPNVLMHCGVDPERYTGFAFGMGVERLAMLRYGVGDLRAFFESDVRFLAQFA"
     gene            4676..>6108
                     /gene="pheT"
                     /locus_tag="VFK00_09400"
     CDS             4676..>6108
                     /gene="pheT"
                     /locus_tag="VFK00_09400"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7593206.1"
                     /translation="MKFSENWLRELVDIAADREALTRRLTMSGLEVGSVESLGGSLEG
                     VVVARIVACEPHPDADRLRVCRVETGSGEVQIVCGAPNAREGLVAPLAAIGATLPNGV
                     TIKAAKLRGIESQGMLCSARDLGIDADVSGLLELPGDAPVGKALANYLGLPDASIEIE
                     LTPNRGDCLGMLGLAFEVAAEFGSNAKPFEPAPVAPAISALREIRLEAGADCPRYLGR
                     AVRGIDMDAATPVWIAQRLRAAGLHPINAVVDVTNYVMLETGQPLHAFDEAKLHGPVV
                     VRRAREGEALKLLDGSEAQLDPGFLVIAGECDPLALAGVMGGFESRVTGETRDVFFEA
                     AHFAPAAVTGRGRKFGLATDAAHRFERGVDPDLPRKAMERATALLMEIAGGQPGEVIE
                     AVWPEHLPRREAVRLRPVRIARVLGITVEDAVVERILAGLGMTVAAQPDGWLVTPPSR
                     RFDIEREEDLIEEVARIHGYGTIPARLP"
BASE COUNT         1152 a         1946 c         2061 g          949 t
ORIGIN      
        1 gtcgcggcgt ccatcggtgc cggtctcgcc aaggccgcac tggccggcaa ggtggacggc
       61 aagctggtgg acctttcgcg cacgatcgac cacgacgcgc gggtggagat cgtcacggaa
      121 aaatcgccgg aggcgctgga cgtcatccgc cactcgaccg cgcacctgct ggcgcaggcg
      181 gtgcagcggt tgtatcccgg cacgcaggtg acgatcgggc cggtcatcga ggatggcttc
      241 tattacgact tcgcgcccag gggcgagccg ttcaagcccg aggacctgcc tgcgatcgaa
      301 gaggaaatgc gcaagatcgc gaaggaatcg ctgcccctct cgcgcagcgt gaagtcgcgt
      361 gacgaggccg tgcagttctt ccgtgggctg ggcgaggact acaaggcgga gatcatcgaa
      421 tcgatcccgg ccaacgagga cctgtcgctg tattcgcagg gcgagttcac cgacctgtgc
      481 cgcggcccgc atgtgcccga taccggaaaa ctcaagtcgt tcaagctgac caagaccgcg
      541 ggcgcctatt ggcgcggcga ttccgacaac gcgatgctca gccgcatcta cggcaccgca
      601 tggctgaatg acaaggacct caaggcgtac ctcacccgga tcgaggaagc cgagaagcgt
      661 gaccaccgca agctcggcaa gcagctcgac ctgttccaca tgcaggaaga ggcgccgggc
      721 atggcgttct ggcacgcgaa gggctggacg ctttggcagc aggtcgagca gtacatgcgg
      781 cgggtctatc gcgactccgg ctaccaggag gtgcgttgtc cttcgatcct ggacgtctcg
      841 ctgtggaaga aatccggcca ctgggacaac tacaaggaca acatgttctt caccgagtcg
      901 gagaagcgca cctacgcggt gaagccgatg aattgcccgg gccacgtaca gatctacaac
      961 gacgggctgc acagctaccg cgacctgccg atccgctacg gcgaattcgg ttcgtgcacc
     1021 cgcaatgagc cctccggtgc actgcacggg ctgctgcggg tgcgcagctt cacccaggac
     1081 gatggccaca tcttctgcac cgaagaccag atcgagcagg aggtggtgga tttccatcgc
     1141 caggcgctga aggtgtacgg ggacttcggt ttcacgaacg tggaattgat gctggcgctg
     1201 cgccccgagt ccaggatcgg cgacgacgcc gtgtgggaca agtcggaaga tgccttgcgc
     1261 gccgccctgc ggcgggccgg cgccgagtgg aaggagctgc cgggcgaggg cgccttttac
     1321 ggtcccaaga tcgaatacca cctgcgcgac gcgatcgggc gagactggca actcggtacc
     1381 atgcaggtcg atttctcgat gccggggcgc ctcggcgccg aatacgtggg cgaggattcc
     1441 cagcgccata ccccggtgat gctgcaccgg gccatcgtcg gctcgatgga acgtttcctc
     1501 gggatcctga tcgagcacca tgccggcaat ttcccaacct ggctggcccc ggtccaggcc
     1561 gtggtgatga acatcaccga cagccaggcc gaatacgcgg agcgggcggc ggcgaacctg
     1621 aacgaggcgg gtttccgggt cgaggccgat ttgcgcaacg agaaaatcgg ctataaaatt
     1681 cgcgagcata cgttgcagaa ggtgccttgg ctgctcgtgg tcggcgaccg cgagaaggag
     1741 aggggcaccg tttccgtgcg tacgcgttcc ggtgaagacc tcggcagcat gtcgctgccc
     1801 gggttcatcc agcgactcga agccgagccg cggccgcgtt gacggatgca cgccaggcat
     1861 cgctggcgga cttgaccagg agaagacggt atcgcaaccg aaaacaaggg caatcggcgc
     1921 aaccaggata tccgcgttcc gcgtgtacgc gtgatcgacg ccgatgggga acaggtcggt
     1981 attctcgatc gcgatgcggc attggaactg gcaggggaag ccggcctgga tctggtcgag
     2041 atccagccca ccgcggatcc gcctgtctgc cggatcatgg acttcggcaa gttcaagttc
     2101 gagcagcaga aaaaggcggc tgcgcagcgc aagaagtcca agcaggtgga gaccaaggag
     2161 ctgaagttcc gcccgacaac ggatgtcggc gactacaaca tcaagctccg caacatggtc
     2221 cggttcctgg aggaaggcga caaggtcaag gtcaacatcc gtttccgtgg acgcgagatg
     2281 cgccaccagg aactcggcat cgaactggcc cagcgcatcc agaaggacat ctcggagacc
     2341 ggtacggtcg accagttccc gcgcatggaa ggccgccaga tgaccatgat gatcagcccc
     2401 aagaagaagt agttgcggag taccacaagc ttttcgaatg cagcgggatt ggtgcgaagc
     2461 gataccgcat ggcaccaaac gttcccctct cctccaaacg gcttcatcgt ttgggggaga
     2521 gggaaaaggt gagggggcaa gcgtttcgta cttgccgaat gtttcggcta gtgcagaaaa
     2581 aagcccccct cacccagcgc tacgcgctcg gcctacggcc acctctcccc cgagcagaaa
     2641 tcgctcgggg gagagggaga caagcattgg cgcttcgaag tttgaggcgc aaaccatccg
     2701 ccgcggatcg gcggacggtc aaagcgacaa cccgaaatgg tcgtcgcaca cacaagccgg
     2761 caagggcatg gatggaaagt gcgatcgtat cgccgcccgg gccagtcacg aaaaccgaaa
     2821 cggagtggca acatgccaaa gatcaagacc aaccgggccg ccgccaagcg gttccgcaag
     2881 accgcgaccg gcaagttcaa ggccggacac gccttcaagt cgcacatcct caccaagaag
     2941 tcgaccaagc gcaaacgcgg cctgcgcgcc cccaaccacg tgcatgagtc cgacaccaag
     3001 cgtgtcgcgc gcatgctgcc atacgtctaa gcggaggaca ccaccatggc acgagtcaag
     3061 cgtggcgtca ccgcgcgtgc gcgtcacaag aaaacgctga aggccgccaa gggctattac
     3121 aacgcacggc gcaaggtcta tcgagtcgcc aagcaggcgg tcaccaaggc ccagcagtac
     3181 gcctacatcg gccgcaagca gaagaagcgc cagttccgcg cgttgtggat cgtgcgcatc
     3241 aacgcggccg cacggcagtt cggactgtcc tacagccggc tgatcgcggg cctcgacaag
     3301 gccggcatca ccgtcgatcg caaggtgctt gccgacatcg cggtgcatga catcgcggcg
     3361 ttcggcgcga tcgccgagaa agccaaggcc agcctggctg cttgatgcat cccggcgttc
     3421 gcgccgggtc gctatgatgc aagcgagcgg gaagagcctt ggctcttccc gctttttgtt
     3481 tgcgaatggg gtcccatgca cacacttgcg caacgcaccg atgaagcgct ggcggcgatc
     3541 gcggcggcgg caaccctgga tgcgctggag gcgcagcgtg tcgcgctgct cggccggaac
     3601 ggcgcgatca ccgcggaatt gaaggcgctc ggcaagttgc cgccggacga gcgcaagatg
     3661 cgcggtgcgg aagtcaatgc cgcacgccag gcactggatg cggcgatcac ggcgcgtcgc
     3721 gcgtcgctcg aacaggcggc cctcgaccag cgcctggcat ccgagcgcgt ggacgtcacc
     3781 cagcccgggc gcgacggggg gcgcggcagc atccaccccc tcacgcgcac gcgggagcgc
     3841 atcgaggcga tcttcgcgca actcggctac tcggtggccg aaggccccga gatcgaggac
     3901 gactggcaca atttcgaggc gctgaacttc cccgcacacc accctgcccg cgcgatgcac
     3961 gacacgttct atttcgacga cggccggctg ctgcgcaccc acacctcgcc ggtgcagatc
     4021 cgcgcgatgc gcggcaagca gccgccggtc cgcatcatcg cgccgggcaa ggtgtaccgc
     4081 agcgattccg accagaccca ttcgccgatg ttccaccagg tcgaaggcct gctggtggac
     4141 gaagcggcga ccttcgccga cctgaagggg acgctggagg ccttcgtcgc ggcgttcttc
     4201 gggaaagcgc tcgagatgcg tttccggccg agctatttcc ccttcaccga gccatcggcg
     4261 gaagtcgaca tccgctggca gcgcgaggac ggcgaagccc gctggctgga ggtgctgggt
     4321 tgcggcatgg tccatccgaa cgtgttgatg cattgcggcg tggatccgga gcgttacacc
     4381 ggctttgcct tcggaatggg tgtcgagcgg ctggcgatgc tgcgttacgg cgtcggcgac
     4441 ctgcgcgcgt tcttcgagag cgacgtgcgg ttcctggcgc aattcgcatg aagaaggtgg
     4501 gaccaggggc cgggaacccg ggaccgggcg aaaagcctgg agtcctgcgc tgcccgaaaa
     4561 cgccgggctt tgccgcattc cgtccaagct gtccgacagg cagagtgcgc atggtcggca
     4621 tgttcccgag tctcgcgcgt catccggtcc ccggtcccct gtccccggtc cccctatgaa
     4681 attctccgaa aactggctgc gcgaactcgt cgacattgcc gccgaccgcg aggcactgac
     4741 caggcgcctg accatgagcg ggctcgaggt gggttcggtc gagtccctcg gcggatcact
     4801 cgaaggcgtg gtcgtcgccc gtatcgtcgc ctgcgagccc catcccgatg cggaccggct
     4861 gcgggtgtgc cgcgtcgaga cgggctccgg tgaggtgcag atcgtgtgcg gcgcgccgaa
     4921 cgcgcgcgag gggctggtcg cgccgctggc cgcgatcggt gcgacgttgc cgaacggcgt
     4981 cacgatcaag gcggcaaagc tgcgcgggat cgaatcgcaa ggcatgctgt gttcggcaag
     5041 ggacctcggt atcgacgccg atgtttcggg cttgctggaa ttgcccggcg atgcgccggt
     5101 cgggaaggcg ctggcgaact atctcggcct gcccgatgcc tccatcgaaa tcgaactcac
     5161 gccgaatcgg ggcgactgcc tcggcatgct cggactggct ttcgaggtgg cggcggaatt
     5221 cgggtcgaac gccaagccgt tcgagccagc tccagtggcc ccggccattt ccgcgctgcg
     5281 cgaaatccgg ctcgaggccg gcgccgactg cccgcgttac ctcggccgcg cggtccgcgg
     5341 catcgacatg gatgccgcga cgcccgtgtg gatcgcgcag cggctgcgcg ccgcgggcct
     5401 gcacccgatc aacgcggtgg tggacgtcac caactacgtg atgctggaaa ccggacagcc
     5461 gctgcacgcg ttcgacgaag cgaagctcca cggcccggtc gtggtgcgac gtgcgcgcga
     5521 aggcgaagcc ctcaagttgc tcgatgggag cgaggcgcag ctcgatcccg gcttcctggt
     5581 gatcgcgggc gaatgcgatc cgctggcgct cgcgggcgtc atgggcggat tcgagtcgcg
     5641 ggtgacgggg gagacgcgcg acgtgttctt cgaagccgcg cacttcgccc cggccgcggt
     5701 cacgggccgc gggcgcaagt tcggcctcgc caccgacgcg gcgcaccgtt tcgagcgcgg
     5761 cgtggatccg gacctgcccc gcaaggccat ggagcgcgcc acagccttgc tgatggaaat
     5821 cgcgggcggc cagccgggcg aagtcatcga ggccgtatgg ccggagcacc ttccgcggcg
     5881 cgaggcggtg cggctgcgcc cggtgcggat cgcgcgggtc ctcggcatca cggtcgagga
     5941 tgccgtcgtc gagcgcatcc tggctgggct gggcatgacc gtcgccgccc agcccgacgg
     6001 ttggctcgtg acgccgccgt cgcggcgctt cgacatcgaa cgggaagaag acctgatcga
     6061 ggaagtcgcc cggatccatg gctacggcac gattcccgcg cggctgcc
//
LOCUS       DASSNH010000253         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1275820, whole genome shotgun sequence.
ACCESSION   DASSNH010000253 DASSNH010000000
VERSION     DASSNH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275820"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFK00_09405"
     CDS             <1..895
                     /locus_tag="VFK00_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HET7593207.1"
                     /translation="VVDEALSVGDEAFQRKCFARIDQIRAGGATVVFVSHAASTVIEL
                     CNRAFLLDRGELLSEGSPKYIVSRYQKLLYSPAALYPSIREWIRAGREADADLSEASR
                     EASASIAHSPTSTSAAVMAAGSADNAIDEPFFEAGLVPKSTFRYDHSGATILDPHVET
                     PDGRRVNVLRPQCEYVYAYRVRIDRDVRNVRCGMLIKSVTGLDLAGCITSWRGEDLPD
                     VEAGSELEVRFRFPCLFASGAYFLNAGVQGSVAGEHVYLDRWIDVAMFKVLHEPARLA
                     TTTLDLGIRPEIRVGSTVEVP"
     gene            892..2445
                     /locus_tag="VFK00_09410"
     CDS             892..2445
                     /locus_tag="VFK00_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593208.1"
                     /translation="MTRPLDSGRRALLVLGMHRSGTSAVARVLNLLGAGLGRELVPPG
                     PDNPEGFWEHAEAVRINDDLLHGLGRTWYGMRDMPERWIDSPPANAARQRIGELIERD
                     FGSSSLCAIKDPRMCLTVPLWIDAFEAKGFEVACLFVVRDPREVVASLHHRNRWSRAS
                     LYLMWVQYLLEAASATGRRRRTLLTYDQLMSDWRGSIARVGRTLQLRWPVAPECAASE
                     AIDGFLDDDYRHHALPPSEADGDGEAMPSLAASLYQACRGIAAGEDHWAAISGLQDAH
                     RDIARLYSTHVEHLVAERKGAERRAQTAETALAEQASVATVVRDTFQALQEKLHARLE
                     SMEQGLKTPLQQLQDELSALSGETEARFERQRERLMAVEARIQRQHALLNTLSLRLEQ
                     AGRDRGVDRPLATEPLPRTDMQKVRAELASSNATVAALLASTSWKMTAPVRWLSTHVL
                     RRPPALVATMALAAPPADPPRNRRFGRFAIRRRAAAAEAMAEQGAAPARDRRDRRDGS
                     PESVAFRWM"
     gene            2485..>3366
                     /locus_tag="VFK00_09415"
     CDS             2485..>3366
                     /locus_tag="VFK00_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HET7593209.1"
                     /translation="MSSRKTNGMVADTMAGAQASRLPQNAETARAAVLDGLFASAAGA
                     RGSEYVPIDDQPADAAKIDVRAIAFYLPQFHRIPENDGWWGRGFTEWTNVSKAVPQFV
                     GHYQPRLPAELGYYDLRVVDVMRRQVELARHYGLEGFCFHYYWFNGRRLLERPLQQFL
                     DHPDIDYPFCVCWANENWTRRWDGLEDDVLIRQEHSPEDDLAFIASLEPMLRDPRYIR
                     VDGRPLVVLYRPSLLPDAAATLERWREHCRRTGIGELFLAMVQFDLEDPRVHGFDAAI
                     EFPPHKLGRGLEPINRRL"
BASE COUNT          547 a         1083 c         1180 g          556 t
ORIGIN      
        1 ggtggtggat gaggcgcttt cggtcgggga cgaggcgttc cagcgcaagt gcttcgcccg
       61 tatcgaccag atccgggcgg gcggcgccac ggtggtgttc gtctcgcacg ccgcgagtac
      121 ggtgatcgag ttgtgcaacc gcgcgttcct gctggaccgc ggcgagttgc tgagcgaggg
      181 cagcccgaaa tacatcgtgt cgcggtacca gaagctcctg tattcgcccg ccgccctcta
      241 cccttcgatt cgagagtgga tccgggccgg gcgggaagcg gatgcggact tgtccgaagc
      301 atcgcgcgag gcgtcggcat ccatcgcgca ttccccgacg agcaccagtg cagcggtcat
      361 ggcggccgga agcgccgaca acgcaatcga cgagccgttc ttcgaggcgg ggctggtccc
      421 gaagagcacg ttccgctacg accacagcgg tgcgaccatc ctcgatcccc atgtcgaaac
      481 gcccgacggt cgccgcgtca acgtcctgcg gccccagtgc gaatacgtat atgcgtaccg
      541 ggtgcgcatc gatcgcgatg tccgcaacgt ccgctgcggc atgttgatca agtcggttac
      601 cggactggac ctggccggtt gcatcacgtc ctggcggggg gaagacctgc ccgacgtcga
      661 agcagggtcc gagctggagg tgcggttccg gtttccatgc ctgttcgcgt cgggggcgta
      721 tttcctcaac gccggcgtgc agggcagcgt cgcgggtgaa cacgtgtacc tggatcgctg
      781 gatcgacgtc gcgatgttca aggtcctgca tgaaccggca cggttggcga ccaccacgct
      841 ggatctcggc atcaggccgg aaatccgcgt cggcagcacg gtggaagtgc catgacgcgc
      901 ccgctcgatt cggggcggcg tgcgttgctg gtgctcggga tgcaccggag cggcacgtcc
      961 gccgtggcac gcgtgctgaa cctgctcggc gccgggctcg gcagggaact cgttcccccc
     1021 ggtcccgaca atcccgaggg cttctgggaa cacgccgaag ccgtcaggat caacgacgac
     1081 ctcctgcacg ggctgggacg cacgtggtat ggcatgcgcg acatgcccga acgctggatc
     1141 gattcgccgc cagcgaatgc ggccaggcaa aggatcggtg agttgatcga gcgtgatttc
     1201 ggatcgtcca gcctgtgcgc gatcaaggat ccgcgcatgt gcctcaccgt gccactgtgg
     1261 atcgatgcct tcgaggcgaa gggcttcgaa gtcgcttgcc tgttcgtcgt gcgcgacccg
     1321 agggaagtgg tcgcgtccct gcaccaccgc aaccgctggt cgcgcgcgtc gctttacctg
     1381 atgtgggtcc agtacctgct ggaggcagca tctgccaccg gacgacggcg caggacgttg
     1441 ctgacctatg accaattgat gtccgactgg cggggcagca tcgcgcgcgt cggccggacc
     1501 ttgcaattgc gctggccggt tgcgccggag tgcgcggctt ccgaggccat cgatggcttc
     1561 ctcgatgacg attaccggca ccatgcgttg ccgcccagcg aagcggacgg cgacggcgag
     1621 gcgatgccga gtcttgccgc ttcgctctac caggcttgtc gcgggatcgc ggcgggcgag
     1681 gaccactggg ccgcgatcag cggcctgcag gatgcgcatc gcgacatcgc ccggctttac
     1741 tccacgcacg tcgagcacct ggttgccgaa cggaagggtg cggagcggcg ggcgcaaacg
     1801 gcggaaaccg ctttggcgga acaggcttcg gtcgccacgg tggttcgcga caccttccag
     1861 gcattgcagg agaagctgca tgcccgcctg gaaagcatgg agcagggctt gaaaacgccg
     1921 ctccagcagt tgcaggacga actgtccgcg ctctccggcg aaaccgaggc ccgcttcgaa
     1981 cggcagcgcg agcgcctgat ggccgtggaa gccaggatcc agcgccagca cgcgctgttg
     2041 aacaccctgt cgctgcggct ggagcaggca ggacgcgacc ggggcgtgga ccggccgctc
     2101 gccaccgaac cgttgcctcg aaccgacatg cagaaggtgc gggccgaact ggcgagcagc
     2161 aacgccacgg tcgcggcact gctcgcgtcg acgagttgga agatgaccgc cccggtgcgc
     2221 tggctgagca cgcacgtgct gcggcgtccg ccggcgctgg tggccacgat ggcgctcgcg
     2281 gcaccgccgg cggatccgcc gcgcaatcga cgcttcggaa ggttcgccat ccgtcgccgc
     2341 gctgccgccg cggaggccat ggcggagcag ggtgccgcgc ccgcccggga caggcgcgat
     2401 cgtcgcgacg ggagtcccga gtcggtggcg tttcgctgga tgtagcgcgg cggcttcgct
     2461 ttgaaatgga aaggtagcga tggaatgagc agccggaaga cgaacggcat ggtcgcggac
     2521 acgatggcgg gcgcgcaggc ttcgcggttg ccacagaatg cggagacggc ccgcgcggcg
     2581 gtcctcgacg gcttgttcgc gtcggctgcg ggagcgcgcg gctcggaata cgtcccgatc
     2641 gacgaccaac ccgccgacgc cgcgaaaatc gacgtgcgcg cgatcgcgtt ctacctgccc
     2701 cagttccacc gcatcccgga gaacgacggg tggtggggac gcggcttcac cgagtggacc
     2761 aacgtgtcca aggcggtgcc gcaattcgtg gggcactacc agccgcggct gcccgccgaa
     2821 ctcggctact acgacctgcg cgtcgtcgac gtgatgcggc gccaggtcga attggcgcgg
     2881 cattacggtc tcgagggatt ctgcttccat tactactggt tcaacggccg gcggttgctg
     2941 gaacgtccgt tgcagcagtt cctggaccat cccgacatcg actacccgtt ctgcgtgtgc
     3001 tgggccaacg agaactggac ccgtcgctgg gacggcctcg aggacgatgt cctgatccgt
     3061 caggagcatt cgccggagga cgaccttgcg ttcatcgcgt cgctcgaacc gatgctgcgg
     3121 gatccgcgtt atatccgggt ggacgggcgt ccgctggtcg tgctgtaccg gccatcgctg
     3181 ctgccggacg ccgccgcaac actggaacgc tggcgcgagc attgtcggag gaccggcatc
     3241 ggcgagctgt tcctcgcgat ggtgcagttc gacctggagg acccgcgggt ccacggcttc
     3301 gatgcggcga tcgagttccc gccccacaaa ctcggccggg ggctcgagcc gatcaaccgg
     3361 cggctg
//
LOCUS       DASSNH010000254         8053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1038856, whole genome shotgun sequence.
ACCESSION   DASSNH010000254 DASSNH010000000
VERSION     DASSNH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038856"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFK00_09420"
     CDS             complement(<1..594)
                     /locus_tag="VFK00_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593210.1"
                     /translation="MSAGDGQRTPPYVRLTQRETPPRSRRKGWALLALVVAGHVLLAW
                     VAWFILRPVHQPRDAREVFQVTLFESPSELPAPPPLVPPPPLPGQPPSAPPRHLHYEP
                     PAKGAMRATLEGVEGPPLELYDSNGQIRMPAATSQKQAPAPAYSAPAIKGSHIYSGKS
                     PVPYEPTRFNKDWAPLNESLGAKTVGRAFDKAVEATTL"
     gene            878..2929
                     /gene="fusA"
                     /locus_tag="VFK00_09425"
     CDS             878..2929
                     /gene="fusA"
                     /locus_tag="VFK00_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7593211.1"
                     /translation="MTAYTTGDIRNVALAGHAGAGKTTLMEALLHAGGALQTPGTIER
                     GSTVSDFDDQEKDRRHSIHSAIASVDTAGCHVNLIDTAGYADFRGETLSALAAVETCA
                     VVVNAGNGIEHGTRRLMHYAKQRRLARMLVVNRIDMDGLDLASLVEELRDEFGAECLP
                     VNLPAEGGAKVVDCFFTASGDSDLGPVAGAHQRIIDQVVEINERLMGRYLEDGEETLS
                     PEDLHSAFEQCLREGHLVPICFTSAEAGTGVAEWLQIACKLLPHPGEANPPPFVKGSG
                     PKAKPIEAKPDPDAHVIADVFKIINDPFVGKLSVFRVWQGTVKKDSQLFIDDGKKPFK
                     VGHLLKLQGKEHIEVDEAIPGDIAGVAKVEDIHFDAVLHDSHDEDAIHLQPLAFPLSM
                     FGLAVEPKHKGQDQKLANALARLAEEDPCLGVEHNKELNETVVRGLSDLHLKVTLARM
                     RDKYGVEVITHPPRIAYRETIGGKAEGHHRHKKQTGGAGQFGEVYLKVEPLPRGEGFE
                     FRDDSKGGVIPNQFIPAIEKGVRQVLEHGAIAGYPMQDVRVVVYDGKYHPVDSKEVAF
                     VSAGRKAFLDAVAKASPIVLEPIVNMDVNVPERFMGDVTGGLASKRARISGTDVLRGG
                     TLVVKAQAPLAEVIGYQTELRAITGGEGRFAMELSHYDPVPAQVQKELTEAYKPKTEE
                     D"
     gene            2972..3529
                     /locus_tag="VFK00_09430"
     CDS             2972..3529
                     /locus_tag="VFK00_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593212.1"
                     /translation="MTLASLWLPIILSAVFVFIASALINMLLRLWHTPDYLRFANEDD
                     VRAAIRAGNPAPGQYSLPWCGPEVMKDPAMQEKFVQGPVGLVNLRRPGPVNMGAYLVQ
                     WLLFCILVSVLCALVALVLPVGADPHPVFHTTALTALLGYSIGLVQSAIWFAWPWKNA
                     FKYMLDGLLYAIITGFTFLWLWPAG"
     gene            complement(3655..4623)
                     /gene="pip"
                     /locus_tag="VFK00_09435"
     CDS             complement(3655..4623)
                     /gene="pip"
                     /locus_tag="VFK00_09435"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET7593213.1"
                     /translation="MHETSRRSLYPEIEPFDSGMLPVSALHTLYYEQCGNPHGKPVVF
                     LHGGPGAGCNAKCRRFFDPAVYRIVLFDQRGCGRSTPHAELRENTTWDLVADIERLRE
                     HLRIAKWQVFGGSWGSTLALAYAETHPEHVTELVLRGIFMLRRAELEWFYQGGCDMLY
                     PDAWEKYLAAIPEAEHGDLMQAYHRRLVSCDPDVRLAAARAWSVWEASTSFLLQNEAY
                     IAASAGDEFALAFAGIENHYFVNGGFFEHDDQLLRNAGRLRDIPGVIVQGRHDVVCPM
                     RSAWDLHRAWPEADLRIVPDAGHSAFEPGNAHELVSATDRFRESTR"
     gene            4700..5932
                     /gene="serA"
                     /locus_tag="VFK00_09440"
     CDS             4700..5932
                     /gene="serA"
                     /locus_tag="VFK00_09440"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7593214.1"
                     /translation="MKTSFPKGDIKIVLLEGIHASAVEAFEAAGYTLVERHDKALPRE
                     QLLEAIADAHIVGIRSRTQLDAEAISEARRLLAVGCFCIGTNQVDLANARGRGVPVFN
                     APYSNTRSVAELVIAEAILLIRRIPERNAECHRGGWAKSAEGSYEVRGKTLGIVGYGH
                     IGTQVGVLAEGLGMHVLFHDIEAKLALGNAVAARDLDDLLARSDIVTLHVPQTPATAG
                     MIGGPQIAGMRTGALLINASRGNVVDIDALAAALDAKRIGGAAVDVFPKEPKGRDEEF
                     VSPLRAFDNVLLTPHVGGSTQEAQLNIGTEVASKLVRYSDNGSTVSAVNFPEVNLPEH
                     AGSRRLLHIHHNVPGVLSRINDVFSQGGVNINAQYLQTAGDLGYVVIDVSAPEAQSES
                     LREQLAAIPGTVRTRLLY"
     gene            complement(6249..6325)
                     /locus_tag="VFK00_09445"
     tRNA            complement(6249..6325)
                     /locus_tag="VFK00_09445"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6289..6291),aa:Pro,seq:ggg)
     gene            complement(6374..6748)
                     /locus_tag="VFK00_09450"
     CDS             complement(6374..6748)
                     /locus_tag="VFK00_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7593215.1"
                     /translation="MLDQGRNNELPAIPAKRYFTIGEVSELCAVKPHVLRYWEQEFPA
                     LNPVKRRGNRRYYQRHDVLMIRQIRALLYDQGFTITGARARLEGQQGKMEASLSHQIA
                     HQMRLELEQLLQLLREQSSRRV"
     gene            complement(6726..7028)
                     /gene="ihfA"
                     /locus_tag="VFK00_09455"
     CDS             complement(6726..7028)
                     /gene="ihfA"
                     /locus_tag="VFK00_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HET7593216.1"
                     /translation="MTLTKAEMAERLFLDVGLNKREARECVDAFFEVLREALERGEQV
                     KLSGFGNFDLRNKNQRPGRNPKTGEEIPISARRVVTFRPGQKLKLRVENHAGSGPQ"
     gene            complement(7030..>8053)
                     /locus_tag="VFK00_09460"
     CDS             complement(7030..>8053)
                     /locus_tag="VFK00_09460"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7593217.1"
                     /translation="EDLIEEVARIHGYGTIPARLPAGAPPAPRDEETILPLAALRAGL
                     AARDYREAICYAFVDPHLLDTWQLDAGAVALANPLSADLAVMRTSLLPGLVEALQRNR
                     NRQQSRVRLFETGVVFAREGAKTGDGDAPVETPMLAAVACGSAHAEQWGEPKRALEFF
                     DIKGDLESLLALSGSPQAWSFDASDLPGWLHPGRSARVLRDGVAVGAVGALHPGLQRR
                     LDLPETLLFEVRSDALRQREVPAAQRLPRFPSIRRDIAIEVDERAAWAEVAGVIRSAL
                     PRMLQELVLFDCFRGPGLSEGRKSLAIGLILQDSSRTLTDEDADRSVADAVARLERDL
                     GAQLRR"
BASE COUNT         1390 a         2706 c         2648 g         1309 t
ORIGIN      
        1 cagcgtggtc gcctcgaccg ccttgtcgaa tgcccgcccc acggtcttgg cgccgaggct
       61 ctcgttgagc ggcgcccagt ccttgttgaa ccgtgttggc tcgtacggaa ccggtgactt
      121 gccactgtag atgtgcgagc ccttgattgc gggcgcgctg tacgcgggcg ccggcgcctg
      181 cttttgcgag gtagcggctg gcatgcggat ctgcccgttc gagtcataca gttccagcgg
      241 cggaccctcc acgccctcga gggtcgcgcg catcgcgcct ttcgcggggg gttcgtaatg
      301 caggtgccgc ggcggcgccg agggcggctg ccccggcaac ggcggcggtg gcaccaacgg
      361 tggcggagcg ggcaactcgg atggcgactc gaacagggtg acctggaaca cttccctcgc
      421 gtcgcgcggc tggtgcaccg ggcgcaggat gaaccaggcg acccatgcca gcaacacatg
      481 gcccgcgacg accagcgcga gcagagccca ccctttgcgc cggctccgcg ggggcgtttc
      541 gcgctgggtg agacgcacgt acggcggcgt gcgctggcca tcgcccgcag acactccggc
      601 tccgggttca cgcgggcgaa attcctgcgc tgggtgtccc tcgctgcgag gcatgcaatc
      661 ggccaatggg cgcaagctgc gcgcgttgaa gtgtagggga agcgttgcgg tcgcccatgc
      721 gtggcgcggc cgcgccacgt ctcccgcagc cacggcggac gcgacttcag accgtccgcg
      781 ccagcgcagg ttccgcgggg caccgccgca acttgaccgg cgccccggtt gggagcatgg
      841 tcagcggacc gccgccaccg gaaaatagcg aggccccatg accgcctata ccaccggaga
      901 catccgcaac gtggccctgg cgggccacgc cggcgccggc aagaccacgc tgatggaagc
      961 gctgctgcac gccggcggcg cgctgcagac gccgggaacg atcgagcgcg gcagcacggt
     1021 gtcggatttc gacgaccagg agaaggatcg ccggcattcg atccactcgg caatcgcatc
     1081 ggtggatacc gcgggttgcc acgtcaacct gatcgatacc gcgggttatg ccgatttccg
     1141 gggcgaaacc ctgtccgcgc tggcggccgt cgagacctgc gcggtggtgg tcaacgccgg
     1201 caacggcatc gagcacggca cccgccgcct gatgcactac gcgaagcagc ggcgcctcgc
     1261 gcgaatgctg gtcgtcaacc gcatcgacat ggatgggttg gacctggcgt cgctcgtcga
     1321 ggaactgcgc gacgagttcg gcgccgagtg cctgccggtg aacctgccgg ccgaaggcgg
     1381 cgccaaggtg gtcgactgct tcttcaccgc gagcggcgat agcgacctcg gtcccgtggc
     1441 gggcgcgcac cagcggatca tcgaccaggt ggtggaaatc aacgagcgcc tgatgggccg
     1501 ctacctcgag gacggcgaag aaacgctgtc gccggaagac ctgcattccg ccttcgaaca
     1561 atgcctgcgc gaggggcacc tggtgccgat ctgtttcact tcggcggaag ccggcaccgg
     1621 tgtcgccgaa tggttgcaga tcgcgtgcaa gctgctacca catccgggtg aggccaatcc
     1681 accgccgttc gtgaaggggt ccggcccgaa ggccaagccg atcgaggcca aacctgatcc
     1741 ggacgcgcac gtgatcgccg acgtgttcaa gatcatcaac gatccgttcg tcggcaagct
     1801 ctcggtgttc cgggtctggc aaggcacggt gaaaaaggat tcgcagttgt tcatcgacga
     1861 cggcaagaag ccgttcaagg tggggcatct cctgaaattg cagggcaagg agcatatcga
     1921 ggtcgacgag gcgatccccg gcgacatcgc cggcgtcgcg aaggtcgaag acatccactt
     1981 cgatgcggtc ctgcacgatt cgcacgacga ggacgcgatc cacctgcagc cgctcgcgtt
     2041 tccgctatcg atgttcgggc tggccgtgga accgaagcac aaaggccagg accagaaact
     2101 cgcgaatgca ctggcgcggc tcgccgagga agatccctgc ctcggcgtcg aacacaacaa
     2161 ggaattgaac gagaccgtgg tgcgcggcct gtcggacctg cacttgaagg tgaccctggc
     2221 gcggatgcgc gacaagtacg gggtcgaggt gatcacccac ccgccgcgca tcgcctaccg
     2281 cgagaccatc ggcggcaagg ccgagggcca ccaccggcac aagaagcaga ccggcggcgc
     2341 tgggcagttc ggcgaggtgt acctcaaggt cgaacctttg ccgcgcggcg agggcttcga
     2401 attccgcgat gattcgaagg gcggcgtgat ccccaaccag ttcattccgg cgatcgagaa
     2461 aggcgtgcgc caggtgctcg agcacggcgc gatcgccggc tacccgatgc aggacgtgcg
     2521 ggtggtggtg tacgacggca aataccatcc cgtcgattcc aaggaagtcg ctttcgtctc
     2581 tgccggcagg aaggcgttcc tcgatgccgt ggccaaggca agccccatcg tgctggagcc
     2641 gatcgtcaac atggacgtca acgtgccgga acgattcatg ggcgacgtta ccggggggct
     2701 cgcgtccaag cgcgcccgca tcagcgggac cgacgtgctg cgcggcggca cgctggtggt
     2761 caaggcgcag gcgccgctcg ccgaagtgat cggctaccag accgagctgc gcgcgatcac
     2821 cgggggcgag ggtcgcttcg cgatggagct gtcgcactac gacccggtgc ctgcccaggt
     2881 ccagaaggaa ctcaccgagg cctacaaacc gaagaccgaa gaagattgag ttcgcgttgt
     2941 cccgaagcac gaacctacga ggagagccgt catgacactc gcttcgctgt ggctgcccat
     3001 catcctgtcc gcggtgttcg tattcatcgc gagcgcactg atcaacatgc tgctgaggct
     3061 ctggcacacc cccgattacc tacggttcgc gaacgaggac gatgtacgcg ccgcgattcg
     3121 tgccggcaat ccggcgcccg gccagtattc gctcccgtgg tgcggcccgg aggtgatgaa
     3181 agaccccgca atgcaggaga aattcgtcca gggcccggtt ggattggtca acctcaggcg
     3241 tccggggccg gtgaacatgg gcgcatacct ggtgcagtgg ctgctgttct gcatcctggt
     3301 atcggtgctg tgcgcactgg tcgcgctggt gctcccggtg ggcgctgatc cgcacccggt
     3361 cttccatacc accgcgctga cggcgctgct gggttattcc atcggactcg tccagagcgc
     3421 catctggttc gcgtggccct ggaagaatgc gttcaagtac atgctcgacg gcctcttgta
     3481 cgcgatcatc accggtttca ccttcctgtg gttgtggcct gctggctgac gccgatcggc
     3541 gctacgaatc tggaatccgc cgaagccagg cgcgggccgt tccccggcgg ctcccgcgcg
     3601 tggcggacct ctaggtgcct tgcagccgcc acgcggtgaa acgacgtggg ccgctcatcg
     3661 cgtactttcg cggaaccggt ccgtcgcgct gaccagttcg tgcgcattgc cgggctcgaa
     3721 cgcggagtgc ccggcatccg gcacgatgcg caggtccgct tcgggccagg cacggtgcag
     3781 gtcccaggcg ctgcgcatcg ggcataccac gtcgtggcgg ccttgcacga tcacccccgg
     3841 gatgtcgcgc aaacgcccgg cgttgcgcag caactggtcg tcgtgttcga agaacccgcc
     3901 gttcacgaag taatggttct cgatgccggc gaaagccagc gcaaattcgt cgccggcgct
     3961 ggcggcgatg taggcctcgt tctgcagcag gaagctcgtc gaggcctccc acaccgacca
     4021 ggcgcgcgcc gcagcgagcc tcacatccgg atcacaggac accaggcgcc ggtggtacgc
     4081 ctgcatcagg tcgccgtgct cggcctcggg aatcgccgcg aggtattttt cccacgcgtc
     4141 gggataaagc atgtcgcagc cgccctggta gaaccattcg agctcggcgc gccgcagcat
     4201 gaagatcccg cgcagcacca gttcggtgac gtgttccgga tgggtttccg cataggcaag
     4261 cgcgagcgtc gagccccacg agccaccgaa cacctgccac ttggcgatcc gcaggtgttc
     4321 gcgcaggcgt tcgatgtcgg ccacgagatc ccaggtggtg ttctcgcgca gttccgcgtg
     4381 cggcgtggaa cgaccgcagc cacgctggtc gaacagcacg atccggtaca ccgccggatc
     4441 gaagaaccgc ctgcacttgg cgttgcagcc ggcgcccggc ccgccgtgca ggaaaaccac
     4501 cggcttgccg tgcgggttgc cgcactgctc gtagtagagc gtgtgcaggg ccgaaaccgg
     4561 cagcatgccg ctgtcgaacg gctcgatctc gggatagagg ctgcggcggc tggtttcgtg
     4621 catgtccatg tgcgggtttc atctgcggcg ggaaccccgc tagcctagca ctccaccggt
     4681 tccggacgcc gccgatcgca tgaagacttc gttccccaag ggcgacatca agatcgtgct
     4741 gctggaaggc atccacgcga gcgcggtgga ggcattcgaa gctgcggggt acacgctggt
     4801 cgaacggcac gacaaggcgc tgccgcgtga gcaactgctg gaggccattg cggacgcgca
     4861 catcgtcggg attcgttcgc gcacgcaact ggacgccgag gcgatctcgg aggcgcggcg
     4921 ccttctcgcg gtcggctgct tctgcatcgg aaccaaccag gtcgacctcg cgaatgcacg
     4981 cggtcgcggc gtgccggttt tcaacgcgcc gtattcgaac acgcgcagcg tcgcggagct
     5041 cgtgatcgcc gaagcgatcc tgctgatacg acggatcccg gagcgcaacg ccgaatgcca
     5101 ccgcggcggc tgggcgaaat ccgccgaagg cagctacgag gttcgcggca agaccctcgg
     5161 catcgtcggc tacggtcata tcggaaccca ggtgggcgtg ctggcggaag gcctgggcat
     5221 gcacgtgcta ttccacgaca tcgaagccaa gctcgcgctc ggcaacgccg tggcggcgcg
     5281 cgacctggat gatttgctgg ctcgctcgga catcgtgacg ctgcacgtgc cgcagacccc
     5341 cgccaccgcc ggcatgatcg gcggtcccca gatcgcgggc atgcgaaccg gcgccctgct
     5401 gatcaatgcc tcccgcggca acgtggtcga catcgatgcg ctggccgctg cgctggacgc
     5461 gaagcggatc gggggcgcgg ccgtggacgt gtttccgaaa gaacccaagg gtcgcgacga
     5521 ggaattcgtc tcgccgctgc gcgcattcga caacgtgctg ctgacgccgc acgtcggcgg
     5581 cagcacccag gaggcccagc tcaacatcgg caccgaagtc gcctccaagc tggtgcgcta
     5641 cagcgacaac ggatcgaccg tgtccgccgt gaactttccc gaggtcaacc tgcccgaaca
     5701 cgccggcagc cggcggctgc tgcacatcca ccacaacgtc cccggcgtgc tgtcgcgcat
     5761 caacgacgtg ttctcgcagg gtggcgtcaa tatcaacgcc cagtacctgc agacggcggg
     5821 cgacctcggc tacgtcgtca tcgacgtgtc cgcgcccgag gcgcaatcgg aatcgctccg
     5881 cgagcagctc gcggccattc cgggcaccgt gaggacgcgg ttgttgtatt gacccggctg
     5941 cggaatgcat cgcgggcaag gaaggctgcg cttcgcgcgg gccggctcct gcgattgtcg
     6001 catcgcggcc catggccgcg acctgccaac cccattatgg aaaggcaaca tcacgtacgc
     6061 gcaccggcgg cgggattact cgcgccttcc tggcgctcgc ccttcgggcc atcgcgcgct
     6121 gaagcgcgcg atgttcgctt cgccatccat ggctacgcag tcgaaccggc accttctacg
     6181 aaggcgccgc ttctcattcc caccttggcc aataacaaaa acgggccacg agggcccgtt
     6241 tttgttattg gtcggggcgg cgggattcga acccacgacc ccctgccccc caggcagatg
     6301 cgctaccagg ctgcgctacg ccccgaccga acccacatga taacggaaca cgaagcgcaa
     6361 ccgcccgatc gcatcacacc cggcgcgacg actgctcgcg cagcaattgc agcaactgct
     6421 cgagttccag gcgcatctgg tgggcgatct ggtgcgacag gctggcttcc atcttgccct
     6481 gctggccctc gagccgcgcg cgggcgccgg tgatggtgaa gccctggtcg tacagcaacg
     6541 cccggatctg ccggatcatc agcacgtcgt ggcgctggta atagcgccgg ttgccgcgcc
     6601 gcttgaccgg gttaagcgcg ggaaattcct gctcccagta gcgcagcacg tgcggcttga
     6661 ccgcacacag ctcgctgact tcgccaatcg tgaaataccg cttggccggg atggcgggca
     6721 gttcgttatt gcggccctga tccagcatgg ttctcgaccc tcagtttcag cttttggccc
     6781 ggacggaacg tcacgacccg gcgcgcggag atcgggatct cctcgccggt cttggggttt
     6841 cgccccggac gctgattctt gttgcgcagg tcgaaattgc cgaaaccgga caacttcacc
     6901 tgctcccccc gctcgagagc ctcgcgcaac acctcgaaga aggcgtccac gcactccctg
     6961 gcttcacgct tgttgaggcc gacgtcgaga aacaggcgct cggccatctc tgccttggtc
     7021 agcgtcatgt catctcctca gctgtgcccc caaatcgcgt tccagccgcg ccaccgcatc
     7081 cgcaaccgac cggtccgcgt cctcgtccgt aagcgtgcgc gaactgtcct gcaaaatcaa
     7141 gcctatggca agactttttc gaccttcgct caatcccggg ccgcggaaac agtcgaacag
     7201 taccagttcc tggagcatcc tcggcagggc cgaacggatc acgccggcga cttcggccca
     7261 ggccgcgcgc tcgtcgacct cgatcgcgat gtcgcggcgg atcgacggga aacgcggcaa
     7321 tcgctgagct gccggaacct cccgttgccg caaggcatcg gagcgaacct cgaagaggag
     7381 ggtttccggc aggtcgagcc gccgctgcag cccgggatgg agggcgccga ccgccccgac
     7441 cgcgacgccg tcgcgcagta cccgcgcgct gcgaccggga tgcagccagc cgggcaggtc
     7501 gctggcgtcg aacgaccacg cctgcggcga tccggacagc gccagcagcg attccaggtc
     7561 gcctttgatg tcgaagaact ccagcgcgcg cttcggctcg ccccactgct cggcgtgtgc
     7621 gcttccgcac gcgaccgccg ccagcatcgg cgtttccacc ggcgcatcac cgtccccggt
     7681 ttttgctccc tccctggcaa acaccacccc cgtctcgaac agccgcacgc gcgactgctg
     7741 ccggttgcgg ttgcgctgga gcgcctcgac cagtccggga agaagcgagg tccgcatcac
     7801 cgccaagtcg gccgacaacg gattggcaag cgccaccgca cccgcatcga gctgccaggt
     7861 gtcgagcaag tgtggatcca cgaacgcgta gcagatcgct tcgcgatagt cgcgtgcggc
     7921 gaggccggcg cgcaaggcgg ccaggggaag gatggtctcc tcgtcgcgcg gcgccggagg
     7981 cgcgcccgcg ggcagccgcg cgggaatcgt gccgtagcca tggatccggg cgacttcctc
     8041 gatcaggtct tct
//
LOCUS       DASSNH010000255         9341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_535619, whole genome shotgun sequence.
ACCESSION   DASSNH010000255 DASSNH010000000
VERSION     DASSNH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535619"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFK00_09465"
     CDS             <1..754
                     /locus_tag="VFK00_09465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7593218.1"
                     /translation="AAMLKSLGVFHPNALGTPPLPHRFEGGGPDLSTRFDAPVLLENT
                     PGHYDSVVEFDHCSDDYEMAVKPFSQPVFDELLALLLPHLVPDARILDPSCGPGIEAI
                     ALARLVPEGEVVAADLSRGMVETAWRNARDAGLKNMAFVQADVGDPPATFEGYFDAIS
                     CCLAFHHYPDGAAAVRAWRKVLRPGGMAFVADPGPKWFNDMSNPWAKLADPGFIQHRT
                     GAEFQALFREAGFKDVYWVEALPGIGITVASA"
     gene            764..1876
                     /locus_tag="VFK00_09470"
     CDS             764..1876
                     /locus_tag="VFK00_09470"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7593219.1"
                     /translation="MVLASTLPYDADVVNDALSCVRAAIAAAIGRYLDARAAESGAYA
                     ELYEVLRDYPFRQGKMLRPTICISAARAVGGAGHSALTMATALELYHNAFLIHDDIED
                     GSESRRGGQTLHRRIGVARAINAGDATNVMSVGLLLKNLDVVGVAKSLHLLHEVELMA
                     RQSAEGQAMELDRISDNVPASSEDDYFAMCTKKTCWYSFITPLRAGLIVGWATGRGGE
                     LAPALEAITRFGAAIGIAFQIQDDLLNLQGEIATYGKEIHGDIYEGKRTLMLNHVLRA
                     SPHATRILEFLATPRPRKTDAEAAFVFSEMRRCGSIERGWQVARAHAELAARLLGEMA
                     FLAKSTPVRTDEDWSCEVTDARFLRELVNYVIYRNL"
     gene            2030..3040
                     /locus_tag="VFK00_09475"
     CDS             2030..3040
                     /locus_tag="VFK00_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593220.1"
                     /translation="MPPVPRVDLEARPGTAIALRLKLANRMMAAVLVRPSVDDWVASD
                     GDILPSGARVEPAYAYLLQGAEVRQTISLRVPLHLEEGSRLTSALRFPGIGNEIVPIS
                     VAILAGDDRVPRSALECPLEVSLPLGDELPRAGGDEFGSAAQASYSLVAGLAGLDMLP
                     ARWLVAELLAAIGQVGEARAETDEGRTLLERLGRTRFFKNGVVAFASSQAPRWILSGL
                     SATSGLHAAMGGTMGQGQLLYIWERWLLGLADIDIERGDAAAEVRMSEGQLQAFAASV
                     GTQADRWFADLLLGLAALSPRIARVLDEIAARAPEPPAPPDEAPDAPPDDVLGEDGSL
                     GR"
     gene            3059..4105
                     /locus_tag="VFK00_09480"
     CDS             3059..4105
                     /locus_tag="VFK00_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593221.1"
                     /translation="MNAILQASELFDRVEALRIPAAPHAAAADWKDWYHFVLWEPANG
                     WRVLANVSLTGGGERASLQYTLVAYAPGQAAGMQGVSRGSPWHAGMVRTEPLSIRGNE
                     VELVLRDRVWHLNLQPPRLDLALQLHARADSTPLLVTEESPFGSGHIGWGLVPRLSVD
                     GELRACGQRVAISDDWFCYQDHNFGRFAWGGDFGWEWLVAHAAVPDGRAFTVVIDLRT
                     DRSHQRSGLPYVFIFAGHELRKVFLGPAMTLQWTWSDRAVLPPRLPGTMATLLADRTE
                     RVPTGLSVRGADERDQLHLQLVFDAHLQVVAPDAIQPAYTRITELSGTTILSMRLDGS
                     SILAHGTAYAEYTR"
     gene            4102..4665
                     /locus_tag="VFK00_09485"
     CDS             4102..4665
                     /locus_tag="VFK00_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593222.1"
                     /translation="MTPAAAEERLNDWLVRLAGLLQHSDVHTADLFRRVVAGRRAVIE
                     IDAARLLVQADDARPVTVNIGPAAPGDAATVRTSGDVLREIIDGRSLLDATIASDRLH
                     VRAGLAELLAFHELVLHVLARAPREPALQALWAEFDAEWSGGQQRCNPIDGQPARHGA
                     LCDATPAGVREARSPLFDPYKPPSADS"
     gene            4985..5350
                     /locus_tag="VFK00_09490"
     CDS             4985..5350
                     /locus_tag="VFK00_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593223.1"
                     /translation="MGGVSIPSHYDITLHGGADMDLDNIHVKEMPQVNMAIKELPPLS
                     IAVTQLPTIRLEMAVKELPTIRLDADVGLKPTRVSMPMNTHVCLKLLGLQLMNLSLCG
                     ENMVIIEPYVPRRAETGCA"
     gene            5433..6203
                     /locus_tag="VFK00_09495"
     CDS             5433..6203
                     /locus_tag="VFK00_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593224.1"
                     /translation="MSNPGSEKLNLKFAALRCEKHAADNARIALRARLAPGETRHARV
                     ALSLDPHTPPGQVRGRATIGGETRDVVINVLEHLEMGVTPDQFVVHGRPGGRITVPVV
                     LTNLGNVPCAVPRVALANISQAAGFDQLFHVAIARSGSEGYQAALDTFAQLLQDSEVQ
                     PPKVLVGTGAHGSLAPGSSIETELTFELPGDLARHRRYLGSFVLGRTVCPLEIAVDAA
                     GPDEHEAADTAKPRATSKASRKAGTRATRTSRKRRNSP"
     gene            6200..6874
                     /locus_tag="VFK00_09500"
     CDS             6200..6874
                     /locus_tag="VFK00_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593225.1"
                     /translation="MSHATVRSMAGSANAIDPEPLLAGLPDLVNTATTGYVDLLERGL
                     KMSRAMFSGSQALFRIPDSCCSVPTRECPPHCVAEIEWDGCGCEAQRAVISVKNTSRE
                     DREYTFSAGSLGPAKVEVAPAKASLAPGESVTLNVTAQGSDAYEPGETYTGELLVRGA
                     YEQCVILKRRISPQAVARLTIEQGDIPERITELQWYRHWQCVEPCPQTKPWSRDPAKP
                     EQRPHT"
     gene            6888..7487
                     /locus_tag="VFK00_09505"
     CDS             6888..7487
                     /locus_tag="VFK00_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593226.1"
                     /translation="MLTHDLQHLLEQSIRANTQLLSRTSGLLKGAALAARNPHRLRGN
                     DVSGLLGDWIRLEIDYLGALSRSNSQYLNDVVSLAESVLASGASASATTDDATALRGP
                     VGSVVAFQFQLDNPNSESVAAAIEAEPWRGQGGGSVGANSIRFEPGSTVVPAGGNATI
                     SGRIAVDDRFKAHEIYDTVIRVAGFPKQVRLRLTVVEPS"
     gene            7815..8120
                     /locus_tag="VFK00_09510"
     CDS             7815..8120
                     /locus_tag="VFK00_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593227.1"
                     /translation="MKKNLELLLMDVSARERALILIVNPGSRSDVGDPVTFGEATCDR
                     YAAREWHATRRDPLHIGRFRQRNNFYSIDLPYFSKWHGPCFSCRRAEGGSFHGDCWR"
     gene            8196..8828
                     /locus_tag="VFK00_09515"
     CDS             8196..8828
                     /locus_tag="VFK00_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7593228.1"
                     /translation="MNKHSFVSRSLAVLAACVLIAPAAANATPYVVTLTQQGSNIVAA
                     GSGSFDLTGLTFEYTTVSVAADMEPDTAYIGNGPTTFITDIDAYGGFTGPTSFGIGTG
                     GYAATGSGDWVGIWGSSAIYGAPTFWVPSGYVSGTPLSNSAIYDSASFASLGLTPGTY
                     VWTWGTGAEQSFTLQIGNAVGVPEPAALGTFGLGLLLIGGFMGLRRRTAQ"
     gene            complement(9030..>9341)
                     /locus_tag="VFK00_09520"
     CDS             complement(9030..>9341)
                     /locus_tag="VFK00_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7593229.1"
                     /translation="LVAEAARKLQADGVKVRAVSMPSWELFGQQDQAYRDSVLPPQVK
                     KRLAVEAGASQGWERWVGDAGATLTVDRYGASAPGELVLEKYGFTVDNVIVQAKALLG
                     G"
BASE COUNT         1607 a         3102 c         3038 g         1594 t
ORIGIN      
        1 tgcggcaatg ttgaaaagcc tgggcgtttt ccaccccaac gccctcggca cgccacccct
       61 gccgcatcgg ttcgaaggtg gcgggccgga cctctcgacg cgtttcgacg cacccgtcct
      121 gctggagaac acgcccgggc attacgactc cgtggtcgag ttcgatcact gcagcgacga
      181 ctacgagatg gcggtcaagc cattctccca acccgtgttc gacgaactgc tggccctgct
      241 gctgccgcat ctcgtgcccg atgcgcggat cctcgatccg tcctgcggac ccggcatcga
      301 agcgatcgca ctggcgcgcc tggtccccga aggagaggtc gtggccgctg atctttcccg
      361 cggcatggtc gagactgcct ggcgcaatgc ccgggatgcg ggactgaaga acatggcctt
      421 cgtacaagca gacgtgggcg atccgccggc gacattcgaa ggctacttcg atgcgatcag
      481 ctgctgcctc gcgttccacc actatccgga cggcgcggct gccgtgcgcg cctggcgcaa
      541 agtgcttcgc ccgggcggca tggcattcgt cgccgatccc ggcccgaagt ggttcaacga
      601 catgagcaat ccgtgggcca agctggccga ccccggattc atccagcacc gtaccggcgc
      661 cgagttccag gcgctgttcc gcgaggcggg attcaaggac gtctattggg tcgaggcgct
      721 gcccggcata ggcatcacgg tggccagcgc gtgagcggcc cccatggtcc tcgcgagcac
      781 gctcccgtac gacgctgatg tagtcaacga cgcattgtcg tgtgtccgcg cggccatcgc
      841 cgccgcgatc gggcggtacc tcgatgcgcg cgccgccgaa tcgggcgcct atgcggaact
      901 ctacgaagtc ctgcgcgact atccgttccg gcagggaaag atgctgcggc ccaccatctg
      961 catcagtgct gcccgcgccg tgggtggagc gggccattcc gcactgacga tggcgacggc
     1021 gctggagctt taccacaacg ctttcctgat ccacgacgac atcgaggacg gttccgagag
     1081 ccggcgcggc ggccagacgc tccatcgccg tatcggtgtt gcacgcgcga tcaacgccgg
     1141 cgacgccacc aacgtgatgt cggtcggctt gctgctgaag aacctcgacg tcgtcggcgt
     1201 cgccaagtcg ctgcacctgc tgcacgaagt cgaactgatg gcgcgccagt ccgccgaggg
     1261 acaggccatg gagctcgacc ggatcagcga caacgtgccc gcctcgagtg aagacgacta
     1321 cttcgcgatg tgcactaaga aaacctgctg gtactcgttc atcacgccgc tgcgcgcggg
     1381 gctgatcgtc ggctgggcga ccggccgcgg tggcgagctg gcgccggcgc tggaggccat
     1441 cacgcgcttt ggcgccgcca taggcatcgc cttccagatc caggacgacc tgctgaacct
     1501 gcagggagaa atcgcgacct acggcaagga aatccacggc gacatctacg agggcaagcg
     1561 caccctgatg ctcaaccacg tgcttcgcgc gagcccgcac gccacgcgca tcctggaatt
     1621 cctggccacg ccgcgcccac gcaagaccga cgccgaagcg gcgttcgtct tttcggaaat
     1681 gcggcgttgc ggcagcatcg agcgcggctg gcaggtggcg cgtgcgcacg ccgaattggc
     1741 agcgcgcctg ctcggcgaaa tggcgttcct cgcgaaatcc acgccagtgc gcacggacga
     1801 ggattggtcc tgcgaggtca ccgacgcacg cttcctgcgc gaattggtga actacgtgat
     1861 ttaccgcaat ctttaggacg ccacggatac attgatcggg cttgccatgg gttccgttgc
     1921 cacgctcgcg ctgtcccaag ccacggcttg atgcccgtgg tccgggcagg gcagcctgca
     1981 cgatcggtcg aattgcattg ccctgcacac ccgaaggagc cggatcgcca tgccgcctgt
     2041 ccctcgcgtc gatctcgaag cccggcccgg cacggccatc gcgctgcgcc tgaagctggc
     2101 caaccgcatg atggccgccg tactggtgcg cccgtccgtc gatgactggg ttgcaagcga
     2161 tggcgacatc ctgccttccg gcgctcgcgt cgagccggcc tacgcgtatc tgctgcaagg
     2221 ggcggaagtc cgacagacca tctccttgcg cgtcccgttg catctcgagg aaggctcccg
     2281 actgacatcg gcactgcgct tccccggcat cggcaacgag atcgtgccca tcagcgtggc
     2341 catcctggcg ggcgacgacc gggtgccccg gtctgcactg gaatgtccgc tcgaggtttc
     2401 cctgccactg ggcgacgaat tgccgcgcgc cggaggggac gagttcggat cggcggcgca
     2461 ggccagctac agcctcgtgg ccgggctggc gggcctcgac atgctgccgg cgcgctggct
     2521 ggtggccgaa ctgctggcgg ccatcggaca ggtcggcgag gcccgcgcgg aaaccgacga
     2581 agggcggacg ttgctggagc gcctggggcg cacgcgtttt ttcaagaatg gcgtggtcgc
     2641 gttcgcgtcg agccaggcac cgcgctggat cctttccggg ctctcggcga cttccggatt
     2701 gcacgcggcc atgggaggaa ccatgggcca gggccagttg ctctacatct gggagcgctg
     2761 gttgctgggc ctggccgaca tcgacatcga gcgcggcgac gccgccgcgg aagtgcgcat
     2821 gagcgaaggg cagctacagg ccttcgccgc ttccgtgggc acccaggccg accgctggtt
     2881 cgccgacctg ttgctgggcc tcgctgcttt gtcaccccgc atcgcccgcg tactggacga
     2941 aatcgccgca cgcgcaccgg aacccccggc gccgccggat gaagcacccg atgcgccgcc
     3001 cgacgacgtg ctgggcgaag acggttcgct gggtcgctga ggcgcgcacg gcgtttccat
     3061 gaacgccatc ctgcaggctt cggagctgtt cgaccgcgtc gaggcattgc gtatcccggc
     3121 ggcgccccat gctgccgccg cagactggaa ggactggtat cacttcgtcc tgtgggagcc
     3181 cgccaacggc tggcgggtcc tcgccaacgt cagcttgacc ggtggcggcg aacgggcctc
     3241 gttgcagtac acgctggtcg cgtatgcgcc gggacaagcc gcggggatgc aaggcgtcag
     3301 ccggggcagc ccgtggcacg ccggaatggt ccgaaccgag cctttgtcga tccgcggcaa
     3361 cgaggtcgag ctggtcttgc gcgaccgggt ttggcacctg aatttgcagc cgccacggct
     3421 ggatcttgcc ttgcaactgc atgcacgggc agattccacc cccctgctgg tcaccgaaga
     3481 atccccgttc ggcagcggcc acatcggttg gggcctggtt ccacggctgt cggtcgatgg
     3541 agaactgcgg gcctgcggcc agcgcgtcgc catctcggac gattggttct gctaccagga
     3601 ccacaacttc ggccgcttcg cgtggggcgg cgacttcgga tgggagtggt tggtggccca
     3661 cgcagcagtc cccgacggcc gagccttcac cgtggtgatc gacctgcgca ccgaccgtag
     3721 ccaccaacgt tccgggttgc cttacgtatt catcttcgcc ggccacgaac tgcgcaaggt
     3781 gttcctgggg ccggcgatga cgctgcagtg gacctggtcc gaccgcgcgg tgctgccacc
     3841 ccggttgccc ggcacgatgg cgacactcct cgcggaccgg acggagcgcg tacccactgg
     3901 gctgagcgtg cgcggcgctg acgagcgcga tcagttgcac ctgcaactcg tgttcgatgc
     3961 gcacctgcag gtggtggccc cggacgccat ccaacccgcc tacacgcgca tcaccgaact
     4021 cagcggaacc acgatcctgt cgatgcggct ggacggttcg agcatcctgg cgcacggcac
     4081 ggcctacgcg gagtacacgc gatgacacct gccgcggccg aagaacgatt gaacgactgg
     4141 ctggtgcggc tggccggcct gctgcagcat tcggacgtgc atacggccga tctgtttcgg
     4201 cgcgtggtgg cggggcgccg cgccgtgatc gaaatcgatg ctgcacggct gctggtgcaa
     4261 gccgacgatg cgcggccggt gaccgtgaac atcggccccg ccgctcccgg cgatgccgcg
     4321 accgtgcgca cgagcggtga cgtgttgcgg gaaatcatcg atggacgttc gttgctggat
     4381 gcgaccatcg cgagcgaccg cctccatgtc cgtgcgggtc tggcggagtt gctggcgttc
     4441 cacgaattgg tactgcacgt gctggcgcgc gcgccacgcg aaccggccct gcaggcgctg
     4501 tgggcggaat tcgatgcaga gtggtccggc ggccagcaac gctgcaaccc gatcgacggg
     4561 caaccggcgc ggcacggcgc gttgtgcgac gcaacaccgg ccggcgtgcg cgaggcgcgc
     4621 agcccacttt tcgaccccta caaaccacca tcggccgatt cttgacaggc gccgcgcgac
     4681 ggctcacgat gcttgccgac gagggagatg accgaatatg aggtcgtttc gtccccatgc
     4741 cgtttgcgcc gcgacggcgc gtctcctgcc ggttcgggat tgtccgtgcg tgcctgtgcc
     4801 tggttcgcag ccgcgcagcc ttctcgtcgg ctccctccct tccccacacg accgggtcaa
     4861 ccgtgcgagc ccgcccgccc tgcgacgggt gcaacgctgg cgacgtagcc atcgcacatt
     4921 cgtgaaggga aaaaacagat tgccgggtcc gtcaaccact gaccactgag tcctggaggg
     4981 tgccatgggc ggtgtttcca ttccttccca ctacgacatc acgcttcacg gtggtgccga
     5041 catggacctg gacaacatcc acgtcaagga gatgccccag gtcaacatgg cgatcaagga
     5101 gctgccgccg ttgagcatcg cggtgaccca gttgccgacg atccggctgg agatggccgt
     5161 gaaggagctc ccgaccattc ggctcgatgc cgacgtcggc ctgaagccca cgcgcgtatc
     5221 catgccgatg aacacgcatg tgtgcctcaa gttgctgggc ctccagttga tgaacctctc
     5281 cttgtgcggc gagaacatgg tcatcatcga gccgtacgtg ccgcgccgcg ccgaaacggg
     5341 ctgcgcatga ggagaagcgt atggcagccg cagagaacgc agaacgcatc gtcctgattg
     5401 gcccccctgc gagcgtgacc ggagtggtcc gcgtgagcaa tcccggcagc gagaagctca
     5461 acctcaagtt cgcggccctg cgttgcgaga aacatgccgc cgacaatgcg cgcattgcgc
     5521 tcagggcgcg attggcgccc ggagaaacgc ggcacgcgcg cgtggccctg tcgctcgacc
     5581 cgcacacgcc cccggggcag gtgcggggcc gcgccaccat cggcggagag acacgcgacg
     5641 tggtcatcaa tgttctcgag catctcgaaa tgggggtgac gcccgaccaa ttcgtcgtgc
     5701 acggccgtcc cggcggccgc atcaccgtac ccgtggtgct gaccaatctc ggcaacgtcc
     5761 cgtgcgcggt gccgcgtgtc gcgttggcga acatttcgca ggcagcgggg ttcgatcagt
     5821 tgttccacgt ggccattgcg cgatcgggct cggagggcta tcaagctgcc ctggacacgt
     5881 tcgcgcaatt gctccaggac agcgaggtcc agccacccaa agtcctggtc ggcaccggcg
     5941 cgcacgggtc gctggcgccg ggtagttcca ttgaaaccga actgacgttc gagctgccgg
     6001 gcgatttggc gcgccaccgg cggtacctcg gcagcttcgt cctgggacgc accgtgtgtc
     6061 cgctggagat cgcggtcgac gcggccggtc cggacgagca cgaagcagcc gatacggcaa
     6121 agccgcgcgc gacgagcaag gcgagccgca aagccggaac acgcgcgact cgcacatcca
     6181 ggaagcggag gaactcccca tgagccatgc caccgtgcga tccatggccg gatcggccaa
     6241 cgcgatcgat cccgaaccct tgttggcggg cctgccggat ttggtgaata ccgccaccac
     6301 cggatacgtc gatcttctgg agcgcggcct gaaaatgagc cgcgcgatgt tttccggcag
     6361 ccaggccctc ttccggatcc ccgacagttg ttgctccgtt ccaacccgcg aatgccctcc
     6421 ccactgcgtc gccgaaatcg agtgggacgg ctgcggttgc gaagcgcagc gtgccgtcat
     6481 ctcggtgaag aacaccagcc gggaagatcg cgagtacacc ttttccgccg gctcgctggg
     6541 gccggccaag gtcgaggttg cgcctgccaa ggcgtcgctc gcgcctggcg agtcggtgac
     6601 gttgaacgtc accgcgcaag gcagtgatgc ttatgagccc ggggagacct acacgggaga
     6661 gctgctggtc cgcggcgcct acgagcagtg cgtgatattg aagcgccgga tcagcccgca
     6721 ggcggttgcc aggctgacga tcgaacaggg cgacatcccc gagcggatca ccgaattgca
     6781 gtggtaccgg cattggcaat gcgtcgaacc ctgcccgcag accaagccgt ggtcccggga
     6841 tccggcgaaa cccgaacaac gtccccacac gtgagaggtt ctaccgcgtg cttacccacg
     6901 acctccagca ccttctcgaa cagagcatcc gggccaacac gcaactcttg tcacggacca
     6961 gcggcctgct gaaaggcgca gcattggccg cacggaatcc ccatcgcctt cgcggcaacg
     7021 atgtcagcgg cctgctcggc gactggatca ggctcgagat cgactacctg ggcgcgctct
     7081 cgcggagcaa cagccaatac ctcaatgacg tggtcagcct ggcggaaagc gtgttggcat
     7141 cgggcgcatc cgcatcggca accaccgacg atgcgactgc gctgagggga cccgtcggca
     7201 gcgtggtcgc cttccagttc cagttggaca acccgaacag cgagtccgtg gcagccgcca
     7261 tcgaagccga accctggcgc ggccagggag gcggcagcgt cggggccaac agtatccggt
     7321 tcgaacccgg atccacagtc gtgcccgctg gcggcaatgc cacgatctcg ggccggattg
     7381 ccgtcgacga ccgcttcaag gcccacgaga tatacgacac cgtcattcgc gtcgcaggtt
     7441 ttcccaaaca ggtcaggctg cgactcaccg tcgtcgaacc cagttgaacg ggcccatcag
     7501 ggacccagca gcgttttcgc ctgcacgatc acctcgtcga cggcgaactt ctcgagcacg
     7561 aattgtcgct cgcgcaggac taccatgacc gccatcgggt cagctgcggc gccgccagcc
     7621 gcaccccacc tcgccgacac ctaccagagc gcctgaaaga aagtggtggg ccgccgccat
     7681 ccgtgcgacg gaaaggcctg caacgccaac gacgtcggcc aggatcaacg cggtggtgcc
     7741 gccgcaagcg ggcgcaaggg atcggtcctc tacaccgctt gcatccctag cgcggccagc
     7801 attcgcaaac cgaaatgaag aagaacctcg agctgctgct gatggacgtc agcgccagag
     7861 agcgagctct gattttgatt gtcaatcccg gatcgcgaag cgatgtcggg gatccagtga
     7921 cgttcggcga ggcgacctgc gatcgatacg cagcgcgcga gtggcacgcg acacgtcggg
     7981 atcctttaca catcgggcgg tttcggcaac gcaacaactt ctattcaatt gatcttcctt
     8041 atttttccaa gtggcacgga ccatgctttt cctgccgccg tgcggagggg ggatcgttcc
     8101 acggagattg ctggcgatga acgccgcact ctcttcgcgc aggtatgccg cctcagcggc
     8161 attcccgctc aatcatctgc gcagaggaga gtcgagtgaa caagcacagc ttcgtttcgc
     8221 ggtcgttggc ggttttggca gcgtgtgtgc tgattgcgcc tgctgcagcc aacgccacgc
     8281 cgtacgtggt cacgctgacg caacaagggt cgaatatcgt cgccgcagga agtggatcgt
     8341 tcgatctgac gggtttgacg tttgagtaca caaccgtctc cgtggcggcc gacatggaac
     8401 ccgataccgc gtacataggc aatggaccca ctacgttcat tacagatatc gacgcgtacg
     8461 gtgggttcac gggacccacg agtttcggga ttggcactgg cgggtacgct gccaccggga
     8521 gtggagactg ggtggggatc tggggcagtt ccgcgattta cggtgcaccc acgttttggg
     8581 ttccgtcggg gtatgtctcg ggaactccgc tttcgaatag cgcgatttac gacagtgcaa
     8641 gcttcgccag ccttggactg acgccgggca cctacgtgtg gacctggggc accggcgcgg
     8701 aacaaagctt cacgcttcag atcggcaatg cggtcggcgt gcccgagccc gcggcactgg
     8761 gcacgttcgg tctgggcttg ctgctgatcg gcgggttcat gggtttgcgg cggcgtacgg
     8821 ctcaatagtc cggccaccgg gcaccggatg cgaacacccc cggcaactgg gggtgttcgt
     8881 ttcagttggc ggatccgggc aagctggtag gagtgcgaca tgcgtgcgat cgcttttcgc
     8941 aagtgcggtc aggccatggg ccgctcctgc aaaagcggta taacctgcac ccgcgttcgc
     9001 ttgttcggaa ggcgagttgt aataacgctt cagcccccca gcaacgcctt cgcctgcacg
     9061 atcacgttgt cgacggtgaa gccgtatttc tcgagcacca gttcgccggg cgccgaggcg
     9121 ccgtagcggt ccaccgtgag cgtcgcgccg gcgtcgccga cccagcgctc ccagccttgg
     9181 cttgcgccgg cttcgacagc caggcgcttc tttacctgcg gcggcaggac cgaatcgcgg
     9241 tacgcctggt cctgttgccc gaacagctcc caactcggca tcgacacggc gcgcaccttg
     9301 acgccgtcgg cctgcagctt ccgtgcagcc tccgcaacga g
//
LOCUS       DASSNH010000256         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1629676, whole genome shotgun sequence.
ACCESSION   DASSNH010000256 DASSNH010000000
VERSION     DASSNH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629676"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2732)
                     /locus_tag="VFK00_09525"
     CDS             complement(<1..2732)
                     /locus_tag="VFK00_09525"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7593230.1"
                     /translation="MVTIVSDGGSLLQQLAETSQFGGGNAAYLDQLYEDWLRDPQSVA
                     PEWRTWFQGLRGTGDVSHADAIARIVAAQQQGRRAGVPMDDAHARKQAGVLRFLTAYR
                     SRGHLAADLDPLAIAPKRPAPDLDLPFHGLSDADLDTEFDCGTFAGGGHRMSLRNLRE
                     RLRRTYVASIGAEFMHISDHNQRRWIYDRLEQASGDAGLDDETRKRVLAKLTAADGLE
                     RYLHTKYVGQKRFSLEGGDSLIPLLDDLIRRAAGDDVHELVIGMAHRGRLNVLVNILG
                     KPPQQLFAEFEGKHDGPDDPAHSGDVKYHMGFSADIAAPADNIHVALAFNPSHLEIVN
                     PVVSGSVRARQIRAHDGSHEHVLPVLIHGDAALSGQGVNMELLNMSKTRGFGVGGTVH
                     IVVNNQIGFTTDAEDGRSTLYCTDVAKMVNAPVLHVNGDDPEAVLQCARLAFDFRQQF
                     HKDVVVDLVCYRRHGHNEADEPSATQPVMYDVIKQLRPTRELYADRLARQGLIAADYG
                     KKLFEDYRAKLDAGKPVTELAPPGTQKFAVDWSRFSHGSLAQPVKTGVARKRLNQLAA
                     RILVWPENVTLQSRVAKIYADRRKMAEGELPLDWGYAENLAYATLIDQGWGLRLVGQD
                     TPRGTFFHRHAELHDQKTGNVYTPLAHVREDVAVEVIDSLLSEMAVMAFEYGFSTADP
                     YTLVIWEAQFGDFANGAQVVIDQFISSGEAKWGRLCGLVLLLPHGYEGQGPEHSSARL
                     ERYLQLCALDNMQVCVPTTPSQMFHLLRRQVLRDCRKPLVALTPKSLLRHKLAVSSLD
                     DLENGSFHLVIPDQRIIDKGANAKKVKRVVLCSGKVYYDLFEEAEKRKLGDVAVVRVE
                     QLYPFPRTEVTAELDKYPAAKEVIWCQEEPQNQGAWFQIRHHLQACTGANRSLH"
BASE COUNT          477 a          948 c          968 g          527 t
ORIGIN      
        1 taatgcaggc tgcgattcgc gcccgtgcag gcctgcaggt ggtggcggat ctggaaccag
       61 gcgccctggt tctgcggttc ctcctggcac cagatcactt ccttcgcggc cgggtacttg
      121 tcgagctcgg cagtgacctc ggtgcgcggg aacggataga gctgttcgac gcgcacgacc
      181 gcgacatcgc cgagcttccg cttttccgct tcctcgaaca agtcgtagta caccttcccc
      241 gaacagagca ccacgcgctt gacctttttc gcattcgcgc ccttgtcgat gatgcgctgg
      301 tcgggaatca cgagatggaa gctgccgttc tcgagatcgt cgagcgacga caccgcgagc
      361 ttgtgccgga gcagcgactt gggcgtcagc gcaaccagcg gcttgcggca gtcgcgcaac
      421 acctgccggc gcagcaggtg gaacatctgc gacggcgtgg tcggcacgca cacctgcatg
      481 ttgtccagcg cgcacagttg cagatagcgc tcgagtcgcg ccgatgaatg ttccggtccc
      541 tggccttcgt agccgtgcgg cagcagcagc accaggccgc acagccggcc ccacttcgcc
      601 tcgccggacg agatgaattg gtcgatcacc acctgcgcgc cgttggcgaa gtcgccgaac
      661 tgggcctccc agatcaccag cgtgtaggga tccgcggtgg agaatccgta ctcgaacgcc
      721 atcaccgcca tttcggacag cagcgaatcg atcacctcga ccgcgacgtc ctcgcgcacg
      781 tgggcgagcg gggtatagac gttgccggtc ttctggtcgt gcaattcggc atggcggtgg
      841 aagaaggtcc cgcgcggcgt gtcctggccg accagccgca ggccccagcc ctggtcgatc
      901 aaggtcgcat aggcgaggtt ctccgcgtag ccccagtcga gcggcaactc gccctcggcc
      961 atcttgcggc gatccgcgta gatcttggcg acccgcgact gcagcgtgac gttttccggc
     1021 cacaccagga tcctcgcggc gagttggttg agccgcttgc gcgccacgcc ggtcttgacc
     1081 ggttgcgcca agctgccgtg gctgaaacgg ctccagtcca ccgcgaactt ctgggtaccg
     1141 ggcggcgcga gttcggtgac gggcttgccg gcatccagtt tcgcgcgata gtcctcgaac
     1201 agcttcttcc cgtaatcggc ggcgatcagc ccctgcctgg cgaggcggtc ggcgtacagc
     1261 tcgcgcgtgg ggcggagctg cttgatcacg tcgtacatca ccggctgggt ggcagaaggt
     1321 tcgtcggcct cgttgtggcc gtggcgccgg tagcagacca ggtcgaccac cacgtctttg
     1381 tggaactgct ggcgaaaatc gaatgccagg cgcgcgcact gcagcacggc ctcggggtcg
     1441 tcgccgttca cgtgcagcac cggcgcgttc accatcttgg cgacgtcggt gcaatacagc
     1501 gtgctgcgcc cgtcctcggc gtcggtggtg aagccgatct ggttgttgac cacgatgtgg
     1561 acggtgccgc cgaccccgaa gccgcgggtc ttcgacatgt tcaggagttc catgttgacg
     1621 ccctggcccg acagcgcggc gtcaccatgg atcaggacgg gcaggacgtg ttcgtgcgac
     1681 ccgtcgtgcg cgcggatctg gcgcgcgcgc accgaacccg aaaccaccgg attgacgatt
     1741 tccagatgcg acggattgaa agccagcgcg acgtggatgt tgtcggcggg cgcggcgatg
     1801 tcggccgaga aacccatgtg gtatttcaca tcgcccgaat gcgcaggatc gtcggggcca
     1861 tcgtgcttgc cctcgaattc cgcgaacaac tgttgcggcg gcttgccgag gatgttgacc
     1921 agcacgttga ggcgcccgcg gtgcgccatg ccgatcacca gttcgtgtac gtcgtcgccg
     1981 gcggcgcggc ggatcaggtc gtccagcagc gggatcagcg aatcgccgcc ttccagcgaa
     2041 aagcgcttct ggccgacgta cttggtgtgc aggtagcgtt ccagtccgtc ggctgcggtc
     2101 agcttcgcca acacgcgctt gcgggtttca tcgtcgagtc ccgcgtcacc gctggcctgt
     2161 tccagccgat cgtaaatcca gcgacgctgg ttgtggtcgc tgatgtgcat gaactcggcg
     2221 ccgatgctgg cgacgtaggt gcggcgcagg cgttcgcgca aattgcgcaa gctcatgcga
     2281 tggccgccgc cggcgaacgt gccgcagtcg aattcggtgt cgaggtcggc gtcggacaag
     2341 ccgtggaagg gcaggtcgag gtcgggcgcg ggacgcttgg gcgcgattgc cagcggatcg
     2401 aggtcggccg caagatggcc gcgtgaacga tatgcggtca ggaaccgcaa cacccccgct
     2461 tgcttgcggg cgtgggcatc atccatcgga acgccggctc gacggccttg ctgctgcgcg
     2521 gcgacgatgc gtgcgatcgc gtcggcgtgg gaaacgtcgc cggtgccgcg cagtccctgg
     2581 aaccacgtgc gccattccgg cgcaaccgac tgcggatcgc gcagccagtc ctcgtacaat
     2641 tgatcgagat aagcggcgtt gccgccgccg aattgcgaag tttccgccaa ctgctgcaac
     2701 aaactgccgc cgtcactcac gatggtcacc acggatagtt gatcgttgtc gaaaggtgcc
     2761 gtcccgcact cttcggcgta cctggttcgg cgcaggtccg gaacaggcct tgcgggaccg
     2821 agcatgcaac cgctcggccc gcgccaacgg gcgtttgacc ccgcctgtcg acggacaccg
     2881 cgcgcgcggt caccagccgc ctcgccgcga cccaaaatta
//
LOCUS       DASSNH010000257         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1166522, whole genome shotgun sequence.
ACCESSION   DASSNH010000257 DASSNH010000000
VERSION     DASSNH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166522"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFK00_09530"
     CDS             complement(<1..618)
                     /locus_tag="VFK00_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET7593231.1"
                     /translation="MSKGTHDYTPDARNAAIKINLNGELVARDEAKVSVFDAGYVLGD
                     GVWEGIRVHDGGLPFLDMHLDRLYEGAKAIDMDIGLTREQLTQRIVETLRANDMRDHV
                     HIRLMVTRGIKATPHQDPRFVIGPPTIVIVPEYKEPLPETVERGIRLFTVHVRRGAPD
                     VQDPKLNSHSKLNCILACIQAQKAGYDEALMLDPHGFVSTCNSTHF"
     gene            complement(661..1356)
                     /locus_tag="VFK00_09535"
     CDS             complement(661..1356)
                     /locus_tag="VFK00_09535"
                     /inference="COORDINATES: protein motif:HMM:NF042613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6796 family protein"
                     /protein_id="HET7593232.1"
                     /translation="MHERIRIAGALGLMGALLLAVGDQCLYFAATSGADFMHGLRGIV
                     AAAPTGRVLFGASLGPIAALLYLAGFWHVYMRLRPRHPRMAAIIAIGLSLCLLAGTAY
                     HVLWGEKALAIKATLGATATAQPVLAALEAQLHAFAANVYLIAEIAGYPSAVLLAIMV
                     ATGRSDYPRWLALLTPVVPLVILQLVAPYTPAPFGSLVAGSASNLSFAVFFAASLATP
                     RKPMHADVSFAGS"
     gene            complement(1349..1798)
                     /locus_tag="VFK00_09540"
     CDS             complement(1349..1798)
                     /locus_tag="VFK00_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7593233.1"
                     /translation="MTERTIGQLAHDAGVGIETIRYYQRIGLIAKPQRAFNGWRRYPE
                     RTLQLLHYLRQGRQLGFTLRELGDLFSQSDIGAPRFCMAFRAAIEAKVAELDSELERL
                     QAHRAQLREFAKSCRQREQSGRCPMLNRLACSGEPPIQRGTDIDGDA"
     gene            complement(1843..2856)
                     /locus_tag="VFK00_09545"
     CDS             complement(1843..2856)
                     /locus_tag="VFK00_09545"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET7593234.1"
                     /translation="MDSADLYREAFVLDCNALESIGTQFDEAARLQDLRDSGVSAIKT
                     TLGGANGTFEQAVADIASAQALIERHPEHFIKVRRHADLERAKPEQKVAVIFSFEAAT
                     MLADKLERIDLFRNLGVLIMQLSYNHATPLGHGCLDGETGGLTGLGRDAIARMNDLGV
                     ALDLSHSNTRTTAEAIAASRKPPLVTHAGCRAVFMHPRNKRDEDMKALADRGGVMGIY
                     MLPFLTPDDRQPMLADYMQHMAHALKICGEDHVGIGSDTAFFTVGDAELKAMADEAES
                     RRKHGVGSPGENRPPYIPDINTPRKLERVADALLKHGYSARVAEKVLGLNFGRAFREI
                     WTA"
     gene            2967..3644
                     /locus_tag="VFK00_09550"
     CDS             2967..3644
                     /locus_tag="VFK00_09550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7593235.1"
                     /translation="MESSGLDNPFWASLHTRHRRLALDAGKVARYPPEFAPFLGVAEA
                     GADVEEGLASLVAPDETVLLLGVAPRLSANWRLESLGELAQMVCGGRIEMPGGPEIVP
                     LGGAQRADALALTALVYPHYFRPRTMELGRYFGMYVDGRLAAMIGERLGTETHTEMSA
                     ICTHPDFEGLGYASRLTAFLTNDTLERGQTPFLHVSLANLRAKALYERLGYRLRRRIP
                     FWSLRRA"
     gene            3819..>4182
                     /locus_tag="VFK00_09555"
     CDS             3819..>4182
                     /locus_tag="VFK00_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7593236.1"
                     /translation="MLLEPISLGELHLRNRIVVSPMCQYSSPDGVPTDWHLVHLGSRA
                     VGGAALVFTEATAVSPEGRISPDDAGIWNDTQAEAWSRIASFIRGQGAAAGMQLAHAG
                     RKASTDAPWRGGGPLDAGQ"
BASE COUNT          675 a         1314 c         1438 g          755 t
ORIGIN      
        1 gaagtgcgtc gagttgcagg tcgagacgaa accgtgcgga tcgagcatca acgcttcgtc
       61 gtagccggcc ttctgggcct ggatgcaggc gaggatgcag ttgagcttcg agtggctgtt
      121 gagcttcggg tcctgcacat cgggcgcgcc gcggcgcaca tgcacggtga acagccgaat
      181 gccgcgctcc accgtttccg gcagcggctc cttgtattcg ggcacgatca cgatggtcgg
      241 cggtccgatc acgaaacgcg ggtcctggtg cggcgtggcc ttgatgccgc gcgtgaccat
      301 caggcggatg tgcacgtggt cgcgcatgtc gttggcgcgc agcgtttcga cgatgcgctg
      361 cgtcaactgt tcgcgcgtga ggccgatgtc catgtcgatg gccttggcgc cttcgtacag
      421 acggtcgagg tgcatgtcga gaaagggcag gccaccgtca tgcacgcgga tgccttccca
      481 caccccgtcg ccgagcacat agccggcatc gaacacggaa accttcgctt cgtcgcgtgc
      541 gaccagttcg ccgttcaagt tgatttttat ggccgcgttg cgcgcgtcgg gcgtgtagtc
      601 gtgggtgcct ttgctcatgg gcctgcctgg agaagtggac ccgccgagtc tatcgcgcga
      661 tcaggagccg gcgaaggaca cgtccgcatg catcggcttt cgcggggtgg cgagtgacgc
      721 tgcgaaaaag acggcgaacg agaggttgct ggcactgccg gctaccagcg agccgaacgg
      781 cgccggggtg taaggcgcaa cgagctgcaa gatcacgagc ggcacgaccg gagtcagcag
      841 tgccagccag cgcgggtagt cgctgcggcc cgtggcgacc atgatcgcca gcagcaccgc
      901 ggatggataa cccgcgatct cggcgatcag gtacacattc gcggcgaaag cgtgcaattg
      961 tgcttcgagc gcggccagca caggctgcgc ggttgcggtg gcgcccagcg tggcttttat
     1021 cgccagcgct ttttcgcccc acaacacgtg atacgccgtg ccggccagga ggcagagtga
     1081 caggccgatc gcgatgatcg ccgccatgcg cggatgccgt ggacgcagcc gcatgtacac
     1141 gtgccagaac ccggcgagat acagcaatgc cgcgatcggc cccaggctcg caccgaacag
     1201 cacgcggccg gtgggcgccg cggcgacgat gccgcgcaag ccgtgcatga aatccgcgcc
     1261 gctggtggcg gcgaagtaca ggcattgatc gccgaccgcc agcagcaatg cgcccatcag
     1321 cccgagtgcg cccgcgatcc ggattcgttc atgcatcgcc atcgatgtcg gttcctcgct
     1381 gaataggcgg ctcgccggaa caggccaggc gattgagcat cgggcaccgc ccggactgtt
     1441 cacgttgcct gcatgacttc gcgaattcgc gcaactgcgc gcgatgagcc tgcaggcgtt
     1501 ccagctcgct atccagctcg gcgacctttg cctcgatcgc agcgcgaaac gccatgcaga
     1561 accggggtgc gccgatgtcc gattggctga aaagatcgcc gagttcgcgc agggtgaagc
     1621 cgagttgccg tccttgccgc aggtaatgca ggagctgcag cgtgcgttcc ggataacgac
     1681 gccagccgtt gaaggcgcgt tgcggtttcg cgatcaagcc gatgcgctgg taatagcgga
     1741 tcgtctcgat gccgacgccg gcatcgtgtg ccaattgccc gatggttcgt tccgtcatgg
     1801 ccttgcctcc gcgacgttct aaacccgtac ctgggtacgg gttcaagctg tccagatctc
     1861 cctgaatgca cggccgaagt tcaggccgag caccttttcc gcgacgcgcg cggaatagcc
     1921 gtgcttcagc agggcatcgg cgacgcgctc cagcttgcgc ggcgtgttga tgtcgggaat
     1981 gtatggcgga cggttctcgc ccggcgaacc gacgccgtgt ttgcgccggg attccgcttc
     2041 gtctgccatc gccttcagct cggcgtcgcc gacggtgaag aacgcggtgt cgctaccgat
     2101 gccgacgtgg tcctcaccgc agatcttcag cgcgtgcgcc atgtgttgca tgtaatcggc
     2161 cagcatcggc tgcctgtcgt cgggcgtcag gaacggcagc atgtagatgc ccatcacgcc
     2221 accgcggtcg gccagcgctt tcatgtcctc gtcgcgcttg ttgcgcggat gcatgaacac
     2281 ggcacggcag ccggcgtggg tgaccagcgg cggctttcgt gatgcggcga tcgcctccgc
     2341 cgtggtccgg gtgttggaat ggctcaagtc cagcgcgaca cccaggtcgt tcatgcgtgc
     2401 gatggcatcg cggccgagcc cggtgaggcc gcccgtttcg ccgtcgaggc agccgtggcc
     2461 cagcggcgtg gcgtggttgt agctcagttg catgatcagc acgccgaggt tgcggaacag
     2521 gtcgatgcgc tcgagcttgt ccgccagcat cgtggcggcc tcgaacgaga agatcaccgc
     2581 gaccttctgc tcgggcttgg cgcgttcgag gtcggcgtgg cgccggacct tgatgaaatg
     2641 ctccgggtgg cgctcgatca atgcctgcgc ggaggcaatg tcggccacgg cttgctcgaa
     2701 cgtgccgttg gcgccgccca gcgtggtctt gatggcgctg acgccggagt cgcgcaggtc
     2761 ctgcaggcgc gccgcctcat cgaactgtgt tccgatcgac tcgagggcat tgcagtcgag
     2821 cacgaaagct tcgcggtaaa gatcggcgct gtccaaggtg atggtggctc cggcgcttgc
     2881 cgaaagcggt gcaacgtatc acgaatgcaa gcgggttggc ggtcggtgcc gccacgatgt
     2941 cgcgcgttcc tcgttaacat cgccggatgg aatcatccgg gctcgacaat ccgttctggg
     3001 catcgctgca cacccggcat cgccgtttgg cgctcgatgc cgggaaggtc gcgcgttacc
     3061 cgcccgagtt cgcccccttc ctcggtgttg ccgaggcagg tgcggatgtg gaggaggggc
     3121 tcgcatcgct ggtggcgccg gatgaaacgg tgctgctgct gggcgtggcg ccgcggctgt
     3181 cggccaattg gcggctcgag tcgctcggcg agctcgcgca aatggtctgc ggcggacgca
     3241 tcgaaatgcc tggcggtccg gaaatcgtgc cgctcggtgg cgcccaacgc gccgacgcgc
     3301 tggcgctcac ggcgctggtg tatccgcatt actttcgccc gcgcaccatg gaactcggcc
     3361 gctatttcgg aatgtacgtg gatggtcggc tggcggcgat gatcggcgaa cgcctcggta
     3421 ccgaaaccca cacggaaatg agcgcgatct gcacccatcc cgatttcgag ggactcggct
     3481 acgcgagccg cctgaccgca ttcctgacca acgacaccct cgagcgcggc cagacgccgt
     3541 tcctgcacgt cagcctggcg aacttgcgcg ccaaggcgtt gtatgagcga ctgggctatc
     3601 ggctgcgtcg caggattccc ttctggtcgc tgcggcgcgc ataagtgcga tcgccggaca
     3661 tatcccggga gtgccatcag ccgtttgggg ggcgatgggc gggagccgcg acctcgttgg
     3721 gcttcgctgc gctcagcgcc aacctacaag agcactcggc attgaaggcg cgttcgcggc
     3781 cctcatgtcg atgggatcag tgcaagccgg gagtcgtgat gctgctggaa ccgatctcgc
     3841 tgggcgaact tcaccttcgc aaccgcatcg tggtatcgcc gatgtgccag tactcgtcgc
     3901 cggacggcgt cccgaccgac tggcacctgg tacacctcgg gagtcgcgcg gtgggtggcg
     3961 cggcgctggt cttcaccgag gcgactgcgg tttcgcccga aggacgcatt tcgccggacg
     4021 acgccggcat ctggaacgat acgcaggcgg aagcctggtc gcggatcgca tcgttcatcc
     4081 gcgggcaggg cgccgctgcg ggcatgcagc tcgcgcacgc ggggcgcaag gccagcaccg
     4141 atgcgccgtg gcgcggcggc ggtccgctgg acgcagggca gg
//
LOCUS       DASSNH010000258        10867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1535052, whole genome shotgun sequence.
ACCESSION   DASSNH010000258 DASSNH010000000
VERSION     DASSNH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535052"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1167)
                     /locus_tag="VFK00_09560"
     CDS             complement(181..1167)
                     /locus_tag="VFK00_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET7593237.1"
                     /translation="MIDREDSPDLFSDIPSRDAAPAAPMEQGPPAAEGKLGERLRAAR
                     EAQGRSLADCAGELHLPIKVLERLERNEFGEAEHFVFLRGALSGYAKLLGLPPGSCDA
                     TLRTVAPPEQPALVSVTRTSTARWLLQRYGTAATYIVLTATIAVPLVLLGVRGGLERP
                     ATRIVSLDQAPTASTQAAGLKSRPSSPDTPASSPDATPLRASMTPFAAIGLTDDTASD
                     GAANRTPVAPAPVPPAVAGGQHTLAITAKSDCWFEITDAGGNKVDSGLLHAGDTRTWH
                     SAGVLHVTVGNADGVSVTRDGQPFALAPYRRANVARFDVFGKTAGPIESNRY"
     gene            complement(1164..1973)
                     /gene="pilW"
                     /locus_tag="VFK00_09565"
     CDS             complement(1164..1973)
                     /gene="pilW"
                     /locus_tag="VFK00_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HET7593238.1"
                     /translation="MRLRSSSVLAVALLALGLAACKHDPGIIKYPTPMSNLENAPTES
                     AQRDKQSAAQTHTQLAAAYMGQNNLKEAETALHKAIGFDSKYIPAHTMLAILDWRINR
                     LQDADKEFRAAISLDPSNGDTNNNYGKFLCETGKRDEAMKYFKRALTDPFYKTPAVAN
                     ANAGMCLLEGNDYAGAEPYLHKALSIDSTYGAALLAMTELDYRKGDAFAARGYLQRFE
                     AAGRATPDSLLLGYRIATRLGDKETATNYANRLQDQFPNSAQAQSLNGSSQ"
     gene            complement(1963..3087)
                     /gene="rlmN"
                     /locus_tag="VFK00_09570"
     CDS             complement(1963..3087)
                     /gene="rlmN"
                     /locus_tag="VFK00_09570"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7593239.1"
                     /translation="MADLARANLLDFDRDGLRNFFAELGEKPWRADQVMKWMYHRLES
                     DFEAMTDLGKSLREKLPEVAVIAPPKALLDNASSDGTRKWLLGMDAGNAVETVYIPEP
                     TRGTLCVSSQIGCGLNCTFCSTGTQGFNRNLAASEIIGQVWHAAKQLGNVTHQQRRIT
                     NVVMMGMGEPLLNFDNVVKAMSLMRDDLGFGLASKRVTLSTAGVVPMIDKLGEVADVS
                     LAVSLHAPNDELRTQLVPLNKRWGIDELLDACARYAARRPRTTITFEYTLMKGINDTP
                     ALAKQLVKRMRRLPGKVNLIPFNPFPGTRYERSDEGTIRAFQTILLDAGVQTMVRRTR
                     GEDIDAACGQLKGRVADRTRRHAEFQRRLAARTEVQTHAA"
     gene            complement(3104..3529)
                     /gene="ndk"
                     /locus_tag="VFK00_09575"
     CDS             complement(3104..3529)
                     /gene="ndk"
                     /locus_tag="VFK00_09575"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7593240.1"
                     /translation="MALERTLSIIKPDAVAKNVIGQIYARFEEAGLRIAAAKMKQLSR
                     AEAEGFYAVHRERPFFKPLVDFMLSGPVMIQVLEGDGAIAKNRELMGATNPKEAAPGT
                     IRADFAQSIDANAVHGSDGPDTARFEIGYFFAATELCSR"
     gene            3754..4374
                     /locus_tag="VFK00_09580"
     CDS             3754..4374
                     /locus_tag="VFK00_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7593241.1"
                     /translation="MHGTPPFSTRQRILGAAEELFAERGFAGASLRQVTAAANVNLAA
                     VNYHFGSKDNLIEEVFRRRLDELSRRRLEQLAALEAAGTPTLEAVLDAFITPALELSL
                     DRRGGSVFMRVLARAFAEHNSHLRRFLSDNYGYVLKDFATAFARLLPQLGKEELYWRL
                     DIAVGALTYAMADFGMIQRRTRDSEREHREQTAKHLIHFTAAGLRA"
     gene            4568..6940
                     /locus_tag="VFK00_09585"
     CDS             4568..6940
                     /locus_tag="VFK00_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET7593242.1"
                     /translation="MPSETLRIRKAAVLGAGVMGAQIAAHLTNAGIPTVLFDLAAKDG
                     PKNGIAVKAIQHLGKMSPAPLALKDLAAAIRPADYEEDLDALKDCDFVIEAIAERMDW
                     KLDLYRKVAAHIPERTILASNTSGLSINQLAEALPENLRHRFCGVHFFNPPRYMHLVE
                     VIPGQATDPKLLEGLEAFLTSTLGKGVVFAKDTPNFIGNRIGVFSILSTMHHTTRFKL
                     GFDAVDALTGPAIGRPKSATYRTADVVGLDTMAHVIETMADTLPDDPWHEHFKSPAWL
                     TALIGKGALGAKVGAGFYRKAGREIQVLDLAKQDYRPSEQKASEEVAAILAIKNPAEK
                     FAKLRESNDPQAQFLWACFRDLFHYTAFHLESIADTARDVDFAIRWGYGWKLGPFETW
                     QAAGWEQVAKWIAEDIAAGKAMSNAPLPAWVTDGRKGVHFADGSYSPRENKDIPRSSA
                     SVYKRQLFPDALIGEKFDQGTTVWENDGIRLWTLDGDDIGIVSFKTKMCTFDDQVLDG
                     IQHAVGLAEQKLKALVVWQTGPVFSAGANLKGVLALLKDGKVSQLEAMIANFQRATMR
                     VQHGLVPTIAAVRGLCLGGGCELQMHSARTVAALESYIGLVEAGVGLLPAGGGLKEIA
                     ERCSRAAHGEDPFDHVKRYFETVAMAKASTGALEARQFGLLRDSDVVVFNANELLHVA
                     KNVASSLAESGWRPPLPRATRVAGDVGTATIKAQLVNMLAGHFISEHDMEVASRIADT
                     LCGGNIERGSMVDAQWLLDLERHHFMELAQMEKSQARIAHTMTTGKPLRN"
     gene            7056..8252
                     /locus_tag="VFK00_09590"
     CDS             7056..8252
                     /locus_tag="VFK00_09590"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7593243.1"
                     /translation="MTKQIQDAYIVAAKRTPVGKAPRGVFRNTRPDDMLAHVLRETLD
                     ECAGIDVNRISDVVIGCAMPEAEQGMNVARIGALLAGLPDTVPGMTINRFCSSGLQSV
                     AIAANTIRLGESNLVLAGGTESMSMVPMMGHKVAMNPDIFKDDNIGIAYGMGITAENV
                     AERWKVSREEQDAFALRSHQRAIAAQDAGNFKEEIAPYPLDDRYPDLRSRGVTEDRRL
                     IENDEGPRRDTSAEALAKLRPVFKQGGSVTAGNSSQMSDGAGAVLLASEQAIKDYGLT
                     PLARFVGYSVAGVAPDIMGIGPKEAIPKALERTGIKRDQLDWIELNEAFAAQALAVIK
                     DLGLDPGKVNPEGGAIALGHPLGATGAIRIATLVHGMRRRNQKYGMVTMCIGTGMGAA
                     GIFEMV"
     gene            8284..8811
                     /locus_tag="VFK00_09595"
     CDS             8284..8811
                     /locus_tag="VFK00_09595"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET7593244.1"
                     /translation="MDVPPPAVVVMGVSGSGKTTIGRALAALLDWCFRDGDEFHGPAN
                     VEKMRAGHPLDDADRAPWLAAIARWIDECAREGVPTVVACSALKLRYRDFLREHRPQV
                     WFFYLRVPRSELERRLQTRDHPYMPGSLLASQLQTFEEPDGREPRTIALDAADDVETI
                     VDVALRELRAQGVCR"
     gene            complement(8862..9347)
                     /locus_tag="VFK00_09600"
     CDS             complement(8862..9347)
                     /locus_tag="VFK00_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HET7593245.1"
                     /translation="MDWNILWYVLAGIIIVAGFIGSILPNLPGIPVMFGGMLLAAWVG
                     HFEKIPVWVVVFLGLLAAFSILFDFLAGTWGAKRYGASKAAVWGAFIGTIIGLFFGIP
                     GIILGPFIGAVAGQLASGSRVEQAARVGVGTWIGLVIGTAIKLATAFMMLGTFALALL
                     I"
     gene            complement(9604..>10867)
                     /locus_tag="VFK00_09605"
     CDS             complement(9604..>10867)
                     /locus_tag="VFK00_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HET7593246.1"
                     /translation="IERERAHPYRPFLHYNSWYDIGYFTPYTEAEALNRINAFGKELV
                     EKRHVKMDSFLFDDGWDDYSGSWHFSKAFLHGFVPLCKATKKYGAAPGVWLSPWGGYG
                     KPRQERIANGRKAGYEIVDGGFALSGPKYWKRFHHVVMTLLDDDCINQFKLDGTGNVD
                     SVYPGSRFDSDFAAAIQLVSDIRADKPDTFINLTTGTWASPFWLRHADSIWRDGYDHN
                     FTGVGPKREQWITYRDEQTYRNIVEKGPLFPINSLMLHGIIFAQHAVGLNTDPGHDFA
                     NEVHSYFATGTDLQEMYISPSLLTDEDWDTLAQAANWSRANADVLRDSHWIGGDPGRL
                     DVYGWAAWSPAKAFITLRNPDNKPQLAVLDVARQLELPKDAAHTFNVSDVWHTGGDAV
                     PKTVDADHPITITLQPFEVLTLQLTPGE"
BASE COUNT         1891 a         3525 c         3573 g         1878 t
ORIGIN      
        1 agatcagcag cagcgcgagc accacgccga cggccacggc gatgccgttc tcccggatcc
       61 acttgcggac ggcttcgccc tgctcgtatt cgtcgtagtc ttcgaaagcc atggttcatc
      121 cacgcacagc gaaaccccgc ctgcgcgggg aaacggccaa ggatacagca gaacgggtcg
      181 tcagtaccgg ttgctttcga tggggccggc ggtcttgccg aacacatcga agcgcgcgac
      241 gttggcgcgc cggtacggtg ccagcgcgaa cggctggcca tcgcgcgtca cgctgacgcc
      301 gtcggcattg cctacggtga cgtgcagcac gcccgccgaa tgccaggtgc gggtgtcacc
      361 cgcatgcagc aggcccgaat cgaccttgtt gccgccggca tcggtgatct cgaaccagca
      421 gtcggatttg gccgtgatcg ccagcgtgtg ctgcccaccg gccaccgcgg gcggaacggg
      481 cgccggtgcg accggggtgc ggttggccgc gccgtcggaa gccgtgtcgt cggtgaggcc
      541 gatcgcggcg aatggcgtca tcgaagcgcg gagtggcgtg gcatccggcg aactcgccgg
      601 cgtgtcaggc gacgacggcc tcgatttcag tccggcggct tgggtggatg ccgtcggcgc
      661 ctggtccagg gaaacgatcc gggtcgccgg ccgctcgagt cccccgcgca cgccgagcag
      721 cacgagcggc acggcgatgg tcgcggtcag gacgatgtag gtcgcggcgg tcccgtagcg
      781 ctggagcagc cagcgcgccg tcgacgtgcg cgtgaccgac accaatgcag gctgctccgg
      841 cggcgcgacg gtgcgcaagg tggcatcgca actgccgggc ggcagcccca gcaacttcgc
      901 gtagccggac agcgcaccgc gcaggaaaac gaaatgctcg gcctcgccga actcgttgcg
      961 ctccagccgt tccagcacct tgatgggcag gtgcaactcg cccgcgcagt cggcgaggga
     1021 gcgcccctgc gcctcgcgcg cggcgcggag gcgctcgccg agctttcctt ccgcagcggg
     1081 cgggccttgt tccatcggag cggccggcgc cgcgtcgcgc gatggaatgt cggagaacag
     1141 gtcggggctg tcttcgcgat cgatcattgg ctggaaccgt tcagcgattg cgcctgcgcg
     1201 gaattcggga actgatcctg caggcggttg gcgtagttgg tggcggtctc cttgtcacca
     1261 aggcgggtgg cgatccggta gccgagcagc agcgagtcgg gggtggcgcg tcctgcggct
     1321 tcgaagcgtt gcaggtaacc acgcgcggcg aaggcgtcgc ccttgcgata gtccagctcg
     1381 gtcatcgcga gcaaggccgc accgtacgtg gaatcgatgg acagcgcctt gtggaggtac
     1441 ggctcggcgc cagcgtaatc attgccttcc agcaggcaca tgccggcgtt ggcgttcgcc
     1501 actgccggtg tcttgtagaa cgggtcggtg agcgcgcgct tgaagtactt catggcctcg
     1561 tcgcgcttgc ccgtctcgca caggaacttg ccgtagttgt tgttggtgtc gccgttcgag
     1621 ggatccaggg agatcgcggc gcggaattcc ttgtcggcgt cctgcagccg gttgatgcgc
     1681 caatcgagga tggcgagcat cgtatgcgcg ggaatgtact tgctgtcgaa accgatcgcc
     1741 ttgtgcaggg cggtctcggc ttccttcagg ttgttctgcc ccatgtacgc cgcggcgagc
     1801 tgggtatggg tctgggcggc cgactgcttg tcccgctgcg ccgattcggt gggcgcgttc
     1861 tcgagattgc tcatcggggt cgggtacttg atgatccccg ggtcgtgctt gcacgccgcc
     1921 agcccgagcg cgagcagcgc caccgccaac acactggaag acctaagccg catgcgtctg
     1981 cacctcggtt cgggccgcca ggcggcgctg gaattcggcg tgtcggcggg tgcggtcggc
     2041 caccctgccc ttgagctggc cgcaggccgc atcgatgtcc tcgccgcggg tgcggcgcac
     2101 catcgtctgc acgcccgcat ccagcaggat cgtctggaat gcccggatgg tgccttcgtc
     2161 ggaccgttcg tagcgggtgc ccgggaaagg attgaacggg atcaggttca ccttgcccgg
     2221 cagccggcgc atccgtttca ccaattgctt cgcgagcgcc ggcgtgtcgt tgatgccctt
     2281 catcaaggtg tattcgaagg tgatcgtggt acgcgggcgg cgcgcggcgt agcgcgcgca
     2341 ggcatcgagc agttcgtcga tgccccagcg cttgttgagt ggcaccagtt gcgtgcgcag
     2401 ctcgtcgttg ggtgcatgca gcgatacagc gagcgacacg tcggcgactt cgccaagctt
     2461 gtcgatcatc ggcaccacgc cggcggtcga gagcgtcacg cgcttggagg cgaggccgaa
     2521 gccgaggtcg tcgcgcatca agctcatcgc cttgacgaca ttgtcgaagt tcagcagcgg
     2581 ctcgcccatg cccatcatca ccacgttggt gatgcggcgt tgctggtggg tgacgttgcc
     2641 gagctgcttc gcggcatgcc acacctgccc gatgatctcg gaggcggcga ggttgcggtt
     2701 gaagccctgg gtgcccgtgg aacagaaggt gcagttgagg ccgcagccga tctgcgagga
     2761 cacgcacagc gtgccgcgcg tgggctccgg gatgtacacg gtctcgaccg cgttgcccgc
     2821 gtccatgccc agcagccatt tgcgggtgcc atcgctggag gcgttgtcca gcaaggcctt
     2881 gggcggagca atgaccgcga cctcgggcag cttttcacgc agcgacttgc cgagatcggt
     2941 catcgcctcg aaatcgcttt cgaggcggtg gtacatccac ttcatcacct gatcggcgcg
     3001 ccaaggcttc tcgcccagtt cggcgaagaa gttgcgcagt ccgtcgcgat cgaaatccag
     3061 cagattggca cgggccaggt cggccatgct gttccccaac gtactaccgg ctgcaaagtt
     3121 ccgtcgcggc gaaaaagtaa ccgatttcga accgcgcggt gtccgggccg tccgagccgt
     3181 gcacggcgtt cgcgtcgatg ctttgtgcga aatccgcccg gatggttccg ggtgccgctt
     3241 ccttcggatt ggttgccccc atcagttcgc gattcttggc gatcgcgcca tcgccttcga
     3301 gcacctggat catcaccgga cccgacagca tgaaatcgac cagcggcttg aagaacggac
     3361 gctcgcggtg caccgcgtag aagccctcgg cttccgcgcg cgagagttgc ttcattttcg
     3421 cggcggcgat cctgagcccc gcctcctcga aacgcgcgta gatctggccg atcacgttct
     3481 tcgcgacggc gtcgggtttg atgatggaaa gggtgcgctc cagcgccatg gggtcctcga
     3541 ctgttgaacg aagggcagga aaaatgcacg cgatgccctt cggcgtccga gcatagtccg
     3601 aaaaacccgc tcaaatgcgg ggttagctca caaaagaatg aagaattcta gctcaacggg
     3661 gccatcttcg ccatgcgccg gcgccccgat tgacccctgc gacgggggga cctagacttc
     3721 aaacgattgt ttgaaatccg agcagggcac ggcatgcacg gcaccccgcc attctccacc
     3781 cgccagcgga tcctcggggc tgccgaggaa ttgttcgccg agcgcggctt cgccggggca
     3841 tcactccgcc aggtcacggc cgcggccaac gtcaacctgg cggccgtcaa ctaccacttc
     3901 gggtccaagg acaaccttat cgaggaagtc ttccgccgcc gcctggacga gctgagccga
     3961 cggcgcctcg agcagctggc cgccctcgaa gcggccggga cgcccacgct ggaagccgtc
     4021 ctggacgcct tcatcacccc tgccctcgaa ctgtcgctgg atcgccgcgg cggctcggta
     4081 ttcatgcgcg tgctggcgcg tgccttcgcc gagcacaaca gccacctgcg ccggttcctg
     4141 tcggacaatt acggctatgt gctcaaggat ttcgccaccg cgttcgcgcg gctgctgccg
     4201 cagctcggca aggaagagct gtattggcga ctcgacatcg cggtcggggc gttgacctat
     4261 gccatggccg atttcgggat gatccagcgg cgtacccgcg acagcgagcg cgaacaccgg
     4321 gaacagaccg ccaaacatct gatccatttc accgcggcgg gcttgcgggc atagcaaagg
     4381 cctgcactgc ttgcatgtcg tcactcccgc gaaagcggga acccagtgtc tttctcgcaa
     4441 cagccggcac ggacaaaaag gctctgggtc ccggatatcg cttttcgatt ccgggatggc
     4501 gagcaaaacc aggggccaca ccgcacaata gtccgttact cgattcatcg atcgctccgg
     4561 agattccatg ccatccgaaa cccttcgcat ccgcaaagcc gccgtgctgg gcgccggcgt
     4621 catgggcgcg cagatcgcgg cccacctgac caacgccggc atcccgacgg tgctgttcga
     4681 cctcgccgcc aaggacggcc cgaagaacgg catcgccgtc aaggcgatcc agcacctcgg
     4741 caagatgtcg cccgcgccgc tcgccctgaa ggacctcgct gccgcgattc gtcccgcgga
     4801 ttacgaggag gacctcgacg cattgaagga ttgcgatttc gtgatcgagg cgatcgccga
     4861 acggatggac tggaagctcg acctctacag gaaggttgca gcacacattc cggagcgcac
     4921 gatcctcgcc agcaacacct cgggcctgtc gatcaaccag ttggccgaag cgttgccgga
     4981 aaacctgcgg catcgcttct gcggcgtgca cttcttcaac ccgccgcgct acatgcacct
     5041 ggtcgaggtg atcccgggcc aagcgaccga tccgaaactg ctcgagggtc tggaagcctt
     5101 cctcacatcc acgctcggca agggcgtggt gttcgcgaag gacacgccga acttcatcgg
     5161 caaccgcatc ggggtgttct cgatcctctc gacgatgcac cacaccaccc gattcaagct
     5221 cgggttcgac gcggtggacg cgctgaccgg tcccgcgatc ggacggccga agtcggcgac
     5281 ctaccgcacc gccgatgtcg tcggcctcga caccatggcg cacgtcatcg agacaatggc
     5341 cgacaccctg cccgatgacc cgtggcacga gcatttcaag tcgcccgcgt ggttgacggc
     5401 gctgatcggg aaaggcgcgc tgggcgcgaa agtcggtgcc ggtttctatc gcaaggccgg
     5461 cagggagatc caggtgctgg atctcgcgaa gcaggactac cgcccgtcgg agcagaaggc
     5521 ttcggaggaa gtcgctgcga tcctcgcgat caagaatcct gccgagaagt ttgccaagct
     5581 gcgcgagtcg aacgatccgc aggcgcagtt cctgtgggca tgcttccgcg acctcttcca
     5641 ctacaccgct ttccacctcg aatcgatcgc cgacaccgcg cgcgatgtcg acttcgcgat
     5701 ccgctggggc tacggctgga agctgggccc gttcgaaacc tggcaggctg ccggttggga
     5761 acaggtggcc aagtggatcg ccgaggacat tgccgccggc aaggccatga gcaatgcccc
     5821 gcttcccgcg tgggtgacgg acggccgcaa gggcgtccat ttcgcggacg gctcgtactc
     5881 gccacgcgag aacaaagaca ttccgcgttc gtcggcgtcc gtctacaagc gccagttgtt
     5941 ccccgatgcg ctgatcggcg agaagttcga ccagggcacc acggtctggg aaaacgacgg
     6001 gatccgcctg tggaccctgg acggcgacga catcggcatc gtcagcttca agaccaagat
     6061 gtgcactttc gacgaccagg tgctggacgg catccagcac gcggtcggcc tggccgagca
     6121 gaagctgaaa gcactggtgg tctggcagac cggcccggtg ttctcggccg gcgccaacct
     6181 caagggcgta ctcgcgctct tgaaggacgg caaggtttcg caactcgaag cgatgatcgc
     6241 caacttccag cgcgcgacga tgcgcgtgca gcacggactg gttccgacca tcgccgcggt
     6301 gcgtgggctg tgcctcggcg gcggctgcga actgcagatg cactcggcgc gcaccgtcgc
     6361 cgccctggaa agctacatcg gcctggtcga agccggcgtc ggcctgttgc ccgccggcgg
     6421 cggcctgaag gagatcgccg aacgctgctc gcgcgcggcg cacggcgaag atccgttcga
     6481 ccacgtcaag cgctatttcg agaccgtggc gatggccaag gcgtcgaccg gcgcactgga
     6541 agcccggcaa ttcggactgc tgcgcgactc cgacgtggtg gttttcaacg ccaacgagtt
     6601 gctccacgtc gcgaagaacg tcgcgtcctc actggccgaa tccggctggc gtccgccgct
     6661 gccgcgggcc acgcgcgtgg ccggcgatgt cggcaccgcg accatcaagg cacagctcgt
     6721 caacatgctg gcggggcatt tcatttccga gcacgacatg gaagtcgcgt cccgaattgc
     6781 cgacaccctg tgcggcggca acatcgagcg cggcagcatg gtcgatgcgc aatggctgct
     6841 cgacctcgaa cgccaccact tcatggaact cgcgcagatg gaaaagagcc aggcgcgcat
     6901 cgcgcacacg atgactaccg gcaagccgct acgaaattga gagcgggaac cgggaacagg
     6961 gaacggggag tgtgcgctcg gttggccctc cgcgctcctc ttcgtctctg ttccctgttc
     7021 cctgatccct gttcccgctt ttggagattc gacccatgac caagcaaatc caggacgcct
     7081 acatcgtcgc cgccaagcgc acgccggtcg ggaaggcccc gcgcggcgtg ttccggaaca
     7141 cccggcccga cgacatgctg gcgcacgtgc tgcgcgagac gctggacgag tgcgccggga
     7201 tcgacgtcaa ccgcatcagc gacgtggtga tcggctgcgc gatgccggaa gccgagcaag
     7261 gcatgaacgt ggcgcgcatc ggtgcgctgc tcgccggcct gccggacacc gtgccgggca
     7321 tgaccatcaa ccgcttctgc tcgtccggat tgcagtcggt cgcgatcgcg gccaacacga
     7381 tccgcctcgg cgaatccaac ctcgtgctcg cgggcggcac cgagtcgatg agcatggtgc
     7441 cgatgatggg ccacaaggtc gcgatgaacc cggacatctt caaggacgac aacatcggca
     7501 tcgcctacgg catggggatc accgccgaga acgtcgccga gcgctggaag gtcagccgcg
     7561 aggagcagga tgccttcgcg ctgcgcagcc accagcgcgc gatcgccgcc caggacgcgg
     7621 gcaacttcaa ggaggagatc gcgccctacc cgctcgacga ccgctatccg gacctgcgct
     7681 cgcggggcgt gaccgaagac cgccgcttga tcgagaacga cgaaggccct cgccgcgata
     7741 ccagtgccga ggcgctggcc aagctgcggc cggtgttcaa gcagggcggc agcgtcaccg
     7801 ccggcaattc atcgcagatg tcggacggtg ccggcgcggt cctgctggcc agcgaacagg
     7861 ccatcaagga ttacggcctg acgccgctcg cgcggttcgt cggctattcg gtcgcaggcg
     7921 tcgcgcccga catcatgggc atcggcccca aggaagcaat cccgaaggcg cttgagcgga
     7981 ccggcatcaa gcgcgaccag ctcgactgga tcgaattgaa cgaggccttt gcggcccagg
     8041 cgctcgcggt gatcaaggat ctcggcctcg accccggcaa ggtcaatccc gaaggcggcg
     8101 cgatcgcgct cggccatccg ctcggcgcca ccggcgccat ccgcatcgcc accctggtcc
     8161 acggcatgcg tcggcgcaat cagaaatacg gcatggtcac gatgtgcatc ggcaccggca
     8221 tgggcgcggc cggcatcttc gagatggtgt gagggcggga ctgctcgatt gtcgtcgtcc
     8281 cccatggatg tgccgccccc ggccgtggtg gtcatgggcg tttccggttc cggcaagacc
     8341 acgatcggcc gcgccctcgc ggcgctcctc gactggtgct tccgcgacgg agacgagttc
     8401 catggtcctg ccaatgtcga aaagatgcgc gctggccatc cgctcgacga cgcggaccgc
     8461 gcgccatggc tggctgccat cgcgcgctgg atcgacgagt gcgcgcgcga gggcgtaccc
     8521 accgtcgttg cctgctccgc gctgaagctg cgctatcgcg atttcctgcg tgagcatcgg
     8581 ccgcaggtgt ggttcttcta tctgcgcgtg ccgcgcagcg aattggagcg gcgactgcaa
     8641 acgcgggatc acccgtacat gccggggtcc ctgcttgcca gtcagttgca aacgttcgag
     8701 gaacccgacg gccgcgagcc gcgaacgatc gcgctcgacg ctgcggatga cgtcgagacg
     8761 atcgtggacg tggcgctgcg cgagttgcgt gcccaagggg tctgccgctg acgtccacga
     8821 acgagcagtt gccggagcgt ttggcttctt tgtacgcgga ctcagatcaa gagtgccagc
     8881 gcgaaggttc ccagcatcat gaacgcggtt gcgagcttga tcgcggtgcc gatcacgagg
     8941 ccgatccagg tgccgacgcc gacgcgcgcg gcctgttcga cgcggctgcc gctcgccagt
     9001 tgaccggcca ccgcaccgat gaacggtccg aggatgatgc cggggatgcc gaagaacagt
     9061 ccgatgatcg tgccgatgaa ggcgccccac accgccgcct tgctggcgcc gtagcgtttg
     9121 gcgccccagg ttccggccag gaagtcgaac aggatcgaga acgcggcgag caggccgagg
     9181 aagacgacca cccataccgg gatcttctcg aaatggccca cccacgcggc cagcagcatc
     9241 ccgccgaaca tcacgggaat ccccggcagg ttgggcagga tcgagccgat gaaaccggcg
     9301 acgatgatga tgccggcgag gacgtaccag aggatgttcc agtccatgct cggaggataa
     9361 cgggtgccgg cttctgtcag tcgttgcccg tcttggctcg tcgtccggag ccggccgcgt
     9421 tgcccatggg gtcgaatcag caacctgcaa ttccgtaggt tgggctgatc ccggacctgt
     9481 tccgggagaa gcccaacagc cgaaaccgcg atgggatgcg acgtcggtgg caagcgtatg
     9541 accgggtttg tcgttgggct tcgctgcgct cagcgccaac ctacaagagc aatctttgct
     9601 tcgctattcc ccgggtgtca attgcagcgt caggacttcg aagggttgca gcgtgatcgt
     9661 aatgggatgg tctgcatcga ccgtcttcgg tacggcgtca ccgcccgtgt gccagacatc
     9721 gctcacgttg aatgtgtgcg cagcatcctt cggcagttcc agttgccgcg cgacatcgag
     9781 gaccgcaagc tgcggcttgt tgtcgggatt acggagcgtg atgaaagcct tcgccggaga
     9841 ccacgccgcc cagccgtaga cgtccagccg tccgggatcg ccgccgatcc agtggctgtc
     9901 gcgcagcacg tcggcgttgg cacgcgacca gttcgccgcc tgcgccagcg tgtcccaatc
     9961 ctcgtcggtc agcagcgacg gagagatgta catctcctgc aaatcggtgc cggtcgcgaa
    10021 ataggaatgc acctcgttgg cgaagtcgtg gccaggatcg gtattcaacc ccaccgcgtg
    10081 ctgggcgaag atgatcccgt gcagcatcag cgaattgatc gggaacagcg gacctttctc
    10141 cacgatgttg cggtaggtct gctcgtcgcg ataggtgatc cactgctcgc gcttgggacc
    10201 cacgccggtg aagttgtggt cgtaaccgtc gcgccagatc gaatcggcat ggcgcagcca
    10261 gaacggcgat gcccaggtcc cggtggtgag gttgatgaaa gtgtccggct tgtcggcgcg
    10321 gatgtccgag accaactgga tcgccgcggc aaagtcgctg tcgaaacggc tacccggata
    10381 cacgctgtcg acgttgccgg tgccgtcgag cttgaactgg ttgatgcagt cgtcgtcgag
    10441 cagcgtcatc accacgtggt ggaagcgctt ccagtacttc gggccggaga gcgcgaaacc
    10501 gccgtcgacg atctcgtagc ccgccttgcg gccattggcg atgcgttcct gccgtggctt
    10561 gccgtagccg ccccacggcg acagccacac gcctggcgcg gcaccgtact ttttcgtcgc
    10621 cttgcacagc ggcacgaaac cgtgcaggaa cgccttggag aaatgccagc ttccgctgta
    10681 gtcgtcccag ccgtcgtcga acaggaacga atccatcttg acgtggcgct tctcgaccag
    10741 ctccttgccg aacgcattga tgcggttgag cgcctccgcc tcggtgtacg gagtgaaata
    10801 gccgatgtcg taccacgagt tgtagtgcag gaacggccgg tacggatgcg cccgttcgcg
    10861 ttcgatg
//
LOCUS       DASSNH010000259         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1166585, whole genome shotgun sequence.
ACCESSION   DASSNH010000259 DASSNH010000000
VERSION     DASSNH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166585"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..139)
                     /locus_tag="VFK00_09610"
     tRNA            complement(63..139)
                     /locus_tag="VFK00_09610"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:His,seq:gtg)
     gene            372..647
                     /locus_tag="VFK00_09615"
     CDS             372..647
                     /locus_tag="VFK00_09615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7593247.1"
                     /translation="MSKPPAEPIPPSEEANAVAQFEQSLDELEQVVAKMEGGELNLDE
                     SLRSFERGIALYRNCQAALEQAEARVKLLLDPAHPETAQSFAPPEER"
     gene            complement(752..1138)
                     /locus_tag="VFK00_09620"
     CDS             complement(752..1138)
                     /locus_tag="VFK00_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET7593248.1"
                     /translation="MSVPPESAVPNPPVAGGSAEDRNLAMLTHLSGIILGFIVPLIVW
                     LVNKDNATKSYLTTEAKEALNFQLTILIGYVICWFLTMTIILAVIGGPLWLILWVVNL
                     VFCIIAAMAVNSKGSYRYPFALRLIK"
     gene            1339..2448
                     /locus_tag="VFK00_09625"
     CDS             1339..2448
                     /locus_tag="VFK00_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HET7593249.1"
                     /translation="MHDQTMALDAGPIEPAASSPPRAHPERPFEFRGSPREYFRIWIV
                     NLALSIATLGIYSAWASVRTRRYFYANTWLAGSPFEYSAQPLPILRGRLIAVLIFACY
                     ILASRVSPWAQLAAAGAVALVMPWLLVRGLAFRARYSSWRGLPFRFEPDFKSAYAWYL
                     AAYLLMLPTAGFAYPWIKKRQQHWMVRHHRYGNAEFDFENKLSSYFEVLFATIGIVLL
                     ALLVLVVFAAIIGLASAGPHGLDLKGHPILVLLAVYAAYIPAYLAVYAYNRSRMLNLL
                     YNGAILGGGHRLRSTLGFREMLWIYLSNLAAIVFTVGLAMPWAKVRMVRYRASNLCLE
                     PAGDLDAFVAETQAQTDVGALGAEMDGFFSIDIGL"
     gene            2445..3536
                     /locus_tag="VFK00_09630"
     CDS             2445..3536
                     /locus_tag="VFK00_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7593250.1"
                     /translation="MTEVAGTYYDGRSSRPHAVAVLCTGAMLHTTGEGVDRTDPLDAV
                     MPSAPLAGVPFTLRFADGARLQVPGDAPVAQWFPRHHRLENRVNRWERRAGIVAAAVL
                     VVALALVALFNWGVPMAADYGAMHLPQSVDRAIGKRTLGVLRAHWLAPSTLPEARRGE
                     LKKRFRDFVARTGDADRLQLEFYRSKTLGANALSLGGGIIVVTDAMVRALPDDDAFLA
                     VTAHEMGHQRYRHMLRTVLRSSGVAVVAGVLAGDVSGSAFATAIPVFLLNAHYSRGFE
                     AQADDFAFHALARAGISPMAFVRAMLALEKAHPELRNDASARYLSSHPVTAGRIARAK
                     AAAQRFERAKAAGKRAGSLARDGHLPDQD"
     gene            complement(3498..>3695)
                     /locus_tag="VFK00_09635"
     CDS             complement(3498..>3695)
                     /locus_tag="VFK00_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7593251.1"
                     /translation="GDYSRGVAGFWVENGKIAHPVQEVTVAGNLRDMFMGVQAIGTDV
                     DPRGSILTGSILVGKMTVAGE"
BASE COUNT          611 a         1243 c         1183 g          658 t
ORIGIN      
        1 attaagagtc tcctgctcta ccaactgagc taacggcccg cgaactccgc attgtacaaa
       61 attggggtgg acgatgggac tcgaacccac gacaaccgga atcacaatcc gggactctaa
      121 ccagctgagc tacgtccacc actgaaactt tcagccaacc gtctcctcag agcgcgccaa
      181 ggcgctgtag ggcgggcact gcccgccggc tcgggtgcgg cggaccatgt ccgccctacc
      241 ctccggcacc ggacgcgcgc agtccacgtt ccgtgcagtg aacgggcaat tttcatcgac
      301 cgggtcgaaa cttgcaagcg ccgcggcgcg gaccgattga ttcaccgcag gccgtgcggt
      361 accgtgcgcg gatgtccaag ccgcctgccg aaccgatccc gccatcggaa gaagccaacg
      421 ccgtcgcgca attcgagcaa tcgctggacg aactcgaaca ggtggtggcc aagatggaag
      481 gcggcgagct gaacctcgac gaatcgctgc gctccttcga gcgcggtatc gcgttgtacc
      541 gcaactgcca ggccgcactg gagcaggccg aggctcgcgt gaagctgctg ctcgaccccg
      601 cgcatccgga aaccgcgcag tcttttgcgc cgcccgagga acgctgacgc agggtgtgct
      661 ggccgcgggg tccgcggcac cacgcccggg atggaccgcg gatctcgacc ggagcgcgcc
      721 tcgcgacgcg ccccgggagc tgcgttcgcc gttacttgat cagccgcaac gcgaagggat
      781 agcgatagct tcccttgctg ttgacggcca tcgcggcaat gatgcagaac accaggttca
      841 cgacccaaag gatgagccaa agcggcccac cgatcaccgc gaggatgatc gtcatggtca
      901 ggaaccagca aatcacgtag ccgatcagga tcgtgagctg gaagttcagc gcttccttcg
      961 cttccgtcgt cagatagctc ttcgtcgcgt tgtccttgtt caccagccac acgatcaacg
     1021 gcacgatgaa cccgaggatg atgccggaca ggtgcgtcag catcgcaagg tttcggtctt
     1081 cggcgcttcc gcctgcaacg ggcgggttgg gtactgcgga ttccggtgga acgctcatcg
     1141 gattgctccc ctctatccag tggtggacgg ttgatgaacc gcgccgtgac tctcgccctc
     1201 ccggcgcggc ctgtcaagcg ccgcgggcca ggtccccgtc gtgcgcggca cggcgctccc
     1261 tgaacaggcc cgcctgcccg gcgccatgcg agtacactcg gggaacggcc aggggtggcc
     1321 gctgccaagg gggaagccat gcacgaccag acaatggcgc tggacgctgg gccgatcgag
     1381 cccgccgcat cttcgccgcc gcgcgcgcat cccgaacggc cattcgaatt tcgcggctcc
     1441 ccccgtgaat atttcaggat ctggatcgtc aacctggcgc tcagcatcgc caccctcggc
     1501 atctattcgg cctgggcatc ggtacgcacc cggcgctact tttacgcgaa tacctggctg
     1561 gctgggtctc cattcgagta ttccgcgcag ccgctgccga tcctgcgcgg gcggctgatc
     1621 gccgtactga tatttgcctg ctacatcctt gccagccggg tttcgccatg ggcgcaattg
     1681 gcggcggccg gtgccgtggc gctggtgatg ccgtggctgc tcgtgcgcgg gctcgcgttt
     1741 cgcgcccgct actcctcatg gcgcggattg cctttccggt tcgagccgga tttcaagagc
     1801 gcctatgcgt ggtacctggc ggcgtacctg ctgatgttgc ccacagccgg gttcgcttat
     1861 ccatggatca agaagcggca gcagcattgg atggtgcgcc accaccgcta cgggaatgcg
     1921 gagttcgact tcgaaaacaa gttgtcgagc tacttcgagg tgctgttcgc gaccatcggc
     1981 atcgtgctcc tggcattgct cgtcctcgtg gtcttcgccg ccatcatcgg cctggcatcg
     2041 gcggggcccc acgggctgga cttgaagggg catcccattc tcgtcctgct cgcggtctat
     2101 gccgcgtaca tacccgccta tcttgccgtc tacgcctaca accgttcgcg catgctgaac
     2161 ctcctctaca acggcgccat cttgggcgga ggccaccggt tgcgcagcac actcgggttc
     2221 cgggaaatgc tgtggattta cctcagcaac ctggcggcga tcgtcttcac ggtcggcttg
     2281 gcgatgccgt gggccaaggt gcgcatggtc cgctatcgcg cgtcgaacct ttgtctcgaa
     2341 ccagcgggcg acctggatgc cttcgtggcc gaaacccagg cgcagaccga tgtcggtgcg
     2401 ctgggcgcgg agatggacgg cttcttcagc atcgacatcg gactgtgacg gaggtcgccg
     2461 gcacttacta cgacggtcgc agcagccggc cccatgccgt cgcggtgctg tgcaccgggg
     2521 ccatgctgca tacgaccggc gaaggagtgg atcgcaccga tccgctcgac gctgtgatgc
     2581 cgagtgcgcc gctggcgggc gtgcccttca cgctccggtt cgctgacggc gcccgcctcc
     2641 aggtgcccgg cgatgctccc gttgcccaat ggtttccgcg ccaccaccgt ctggaaaacc
     2701 gggtcaatcg ctgggagcgc cgcgccggca tcgttgccgc cgcggtactg gtcgtggcgc
     2761 tggcgttggt cgccttgttc aactggggcg tgccgatggc cgccgactac ggggccatgc
     2821 acctgccgca gtcagtggac cgcgcgatcg gcaagcggac actcggcgtg ctgcgtgcgc
     2881 actggctggc accgagcacg ctgccggaag cacgccgcgg cgaactgaaa aaacgcttcc
     2941 gcgatttcgt cgcccgcacc ggcgatgcgg acaggttgca actggagttc tatcgatcga
     3001 agaccctggg ggccaacgcc ttgtcgctcg gcggcggcat catcgtggtg acggatgcca
     3061 tggtgcgcgc gctccccgac gacgacgcgt tcctggcggt caccgcccac gagatgggcc
     3121 accagcgtta ccgccacatg ctgcgcacgg tgctgaggag ttcgggagtg gcggtcgtgg
     3181 caggggtgct ggccggcgac gtgagtggca gcgcgttcgc cacggcgatc ccggtgttcc
     3241 tgctgaacgc acactactcc cgcggcttcg aagcgcaggc cgacgatttc gccttccacg
     3301 cgctggcgcg ggcgggcatt tcaccgatgg cattcgtgcg cgcgatgctg gcgctcgaaa
     3361 aggcgcatcc ggaattgcga aacgatgcca gcgcccgcta cctgtcctcg catccggtca
     3421 cggccggacg catcgcacgc gccaaggccg ccgcgcaacg gttcgaacgc gcgaaggctg
     3481 cgggaaagcg cgcaggatca ctcgcccgcg acggtcatct tcccgaccag gattgagccg
     3541 gtgaggatcg agccgcgcgg atccacgtcg gtcccgatgg cctgcacgcc catgaacatg
     3601 tcgcgcaggt tgccggcgac cgtcacttcc tgcaccggat gggcgatctt gccgttctcg
     3661 acccagaatc ccgccacccc gcgcgaatag tcgcc
//
LOCUS       DASSNH010000260        13675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1145496, whole genome shotgun sequence.
ACCESSION   DASSNH010000260 DASSNH010000000
VERSION     DASSNH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 13675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13675
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145496"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFK00_09640"
     CDS             <1..996
                     /locus_tag="VFK00_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7593252.1"
                     /translation="VGDLFTRGEEQIITALPLYHIFSLTVNALFMTYFGGENVLITNP
                     RDIPAFIKTLKQNRWTAITGVNTLFNALVHHPDFASIDFSRVKFSLAGGMAVQRAVAE
                     RWKKATGIPLIEGYGLTETSPVVTANPLDVKEYSGSIGMPLPSTDVVLRDDGHEAPPG
                     EPGELCVRGPQVMKGYWNRPEETANVMDGDGWLRTGDIARMDEKGFLYIVDRKKDMIL
                     VSGFNVYPNEIEDVVVRHPGVLEAAAIGVPDEHSGEVVKLFVVKKDPALTEAALKQYC
                     HDELTGYKRPKYIEFRDELPKSNVGKILRRELRDEGKEREQGTGNWEQKSSPPAR"
     gene            1009..1467
                     /locus_tag="VFK00_09645"
     CDS             1009..1467
                     /locus_tag="VFK00_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7593253.1"
                     /translation="MPRIDIEATPRHTGCGYPPPFDEPCRPRIRQRLGDAGGLTDFGV
                     NLLELAPGAWSSQRHWHSAEDEFVWVLDGEVTLVDDGGEHVLHAGECAAFPKGDANGH
                     HLVNRSGATVRCLEIGSRKPGADACDYPDVDLRIGPGRDAGYTHKDGSRY"
     gene            1674..2831
                     /locus_tag="VFK00_09650"
     CDS             1674..2831
                     /locus_tag="VFK00_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593254.1"
                     /translation="MKRIATTVLVAGLMATPMLALPPPAKAAISVGISVNLAPPPLPV
                     YGQPAVPGPGYIWTPGFWAWDPGFGYYWVPGTWVMPPQIGLLWTPGWWGWSDGYYRWH
                     PGYWGRHVGFYGGINYGHGYFGAGYVGGEWRGRDFYYNRAVNNVSVANIHNVYVNRTV
                     VRNVHVDRVSYNGGRGGITAHPTAAQRAVANARRWSPTSMQERQREVAKGNPGQRFKA
                     HQGRPAVFATQHAGKFEGPHAVHAPSAPRGYATGASNHASRAAPQPHPANTPDRARTM
                     VQAPRERTHPAPNNVPNRGHAPVQAPRERARPVQQQPMQPQPSFRNAPVHGNARPNPS
                     HPATLQNAPRNAPPHNGQSSRGQPPASSSHGNDRGGKPQNDKHKHDGGHRN"
     gene            complement(2898..3287)
                     /locus_tag="VFK00_09655"
     CDS             complement(2898..3287)
                     /locus_tag="VFK00_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7593255.1"
                     /translation="MFEVAGDYNTDRTVDHVRHARMVRETAVLMSRRGDVWQTDLADI
                     SATGVRLARPTEWHGSSGDVWVLDMLFSDDINVHVMAEVVRVTAAHVALAFARIPEEV
                     QVSLWNLLGGYADTLERWDDELDAIRG"
     gene            3796..4641
                     /locus_tag="VFK00_09660"
     CDS             3796..4641
                     /locus_tag="VFK00_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HET7593256.1"
                     /translation="MSPASAPEASAATAAPRQEEMPLAVVLGQPVLRIPQDLYIPPDA
                     LEVILEAFEGPLDLLLYLIRRQNLDILDIPVAEITRQYMDYIDVMREMRLELAAEYLV
                     MAAILAEIKSRLLLPRPPQEEGIEEDPRAELVRRLQEYERFKKAAEDIDAMPRLERDL
                     ENTRAFVPDHKVVKLPPPVELREMLLALRDVLHRAELYGHHAVEREPLSVRQRMSDVL
                     RKLGDGAFHRFETLFEATEGRLGVVVTFLSLLELAKDRLVDIMQEVPLAPIYLKARAT
                     AETAD"
     gene            4875..5552
                     /gene="scpB"
                     /locus_tag="VFK00_09665"
     CDS             4875..5552
                     /gene="scpB"
                     /locus_tag="VFK00_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7593257.1"
                     /translation="MEQEKLKNIVEAALLAAHQPLTLPMLAALFQDTDAVSHEDLARA
                     VETLGQACEGRGIELQEVASGFRYQVREDVHPWVSRLWAERQTKYSRALLETLALIAY
                     RQPITRGEIEQVRGVAVATSIMRTLEEREWVRVVGYRDVPGKPALFGTTRQFLDYFNL
                     KSLDQLPTLAEIREIADPDPQLALASSRDDAGNPPDAPSDPSDGTDEPAGADAPADAP
                     RTTEDAA"
     gene            5549..6781
                     /locus_tag="VFK00_09670"
     CDS             5549..6781
                     /locus_tag="VFK00_09670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7593258.1"
                     /translation="MSSAPRSLLTLKRSSAHEADGALEERLHKVLANAGLGSRRMLEE
                     RIGAGEILVNGSVATTGSSVRAGDRVTVDRKQFVVATDTHERAAVLMYHKPEGELTTR
                     DDPEGRPTVFENLPHLNGSRWISVGRLDANTTGLLLLTTDGELANTLMHPSHELEREY
                     LCRVHGEVPDEALEKLKAGVELDDGPARFDEIHVVNRGGSHCWFRVVMHEGRNREVRR
                     LWDSQGFLVSRLKRIRYGNVELPRGLRPGQNEMLAEDAVRSLRELAGAGPEQPTLTLK
                     PVLHQRHTPRGVTEVRPADQTPHAWTGVRHDEARELAAFDRVRPDDFNPRGKRRRGPP
                     RGDVNGNVLKPEKPSGRKRKNRRGVAPGQELPSVRTWFAGDKRPGERQGRRPGGGKRG
                     PGGGRGPGGGKGRNGPRG"
     gene            6795..7154
                     /gene="arsC"
                     /locus_tag="VFK00_09675"
     CDS             6795..7154
                     /gene="arsC"
                     /locus_tag="VFK00_09675"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464934.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HET7593259.1"
                     /translation="MSTRPGVTIWHNPRCSKSRGTLELLQGRGIEPAIVDYQKTPPST
                     SEIARALELLGIEPRDLMRKGEAAYAELGLEDPKFTREQLIDAMATHPILIERPVVFA
                     NGKAALGRPPANVLAIL"
     gene            complement(7317..8123)
                     /locus_tag="VFK00_09680"
     CDS             complement(7317..8123)
                     /locus_tag="VFK00_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7593260.1"
                     /translation="MQALRVSLVQGATRWHDPAANRDYYASLIAPLAGRTDLVVLPET
                     FTSGFSNEAIHDAETMEGPSVAWLKEQAAALDAAVTGSMQLRAGEGPAADKVFNRLLF
                     VTPDGALRHYDKRHLFRYADEHKRYAAGGERLVVEWRGWRICPLVCYDLRFPVFSRNR
                     HHAGQGFDYDLALFVANWPAARAHAWRMLLRARAIENLAYCAGVNRVGTDGNQLPYSG
                     DSAVLDFLGQTMADIGAQEQTVTVALDPDALAAFREKFPAWMDADAFELT"
     gene            complement(8239..9384)
                     /locus_tag="VFK00_09685"
     CDS             complement(8239..9384)
                     /locus_tag="VFK00_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7593261.1"
                     /translation="MQIETKLPKVGTTIFTVMSQLALEHKAVNLGQGFPDFETPRNLR
                     EALTRAMEEGRNQYAPMSGIPKLREQIALQVDRLYGRRISADTEVTVTSGATEAIFAA
                     IACAVHADDEVIVLDPCYDCYEPAIALAGGKAVHVPLALPDFAVDWQRVRDALTPKTR
                     MIMVNSPHNPSGAIFEQHDLDALAEIVTRHGLLVISDEVYEHIVFDGARHMSASRKPE
                     LAERSFVISSFGKTWHCTGWKVGYCVAPKPLTAEFRKVHQYLTFCTFHPAQCAFAEVM
                     EKDAQHPGELAAFYQDKRDRFRELLSGSRFVLPAVRGGYFQLADYSEIRDTDDLNFCE
                     WMVREAGVAAIPVSAFYDAPPAAKIVRFCFAKNDDTLSAAAERLCKL"
     gene            complement(9485..10477)
                     /locus_tag="VFK00_09690"
     CDS             complement(9485..10477)
                     /locus_tag="VFK00_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET7593262.1"
                     /translation="MPAANGSLPDLNDFYFFAAVVEHGGFSAASRALNVPKSRLSKRV
                     SQLEDKLGVRLLQRTTRKIAVTEAGERFYRHCQGMLAEARAATEAAAELAGEPRGVVR
                     ISCPVSVAQSLLARHLPVFLAEHPEVQVRLFVSNRRVDIIGEGFDVAIRVREKLDTDA
                     LLVARSFGALRVVLVASPGFLAEHGEPASPHALSTLPVLSMFEHEGDQAWDLYDRTCS
                     KTAVAVKPRLITGDFRVLIEAAAQGIGIAWVPESSCADELRSGRLRIVLPEWGLPQGT
                     LHMVYPSRRGMLPAVRALVDFLAETFARDVRDGSMAGGGSGHAASSPRPTNGHR"
     gene            10589..11170
                     /locus_tag="VFK00_09695"
     CDS             10589..11170
                     /locus_tag="VFK00_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7593263.1"
                     /translation="MKLLHIDSSVLGENSASRSLSAAIVARLRREHRDLEVAYRDLAA
                     QTLPHFTPALADGHPCVSRNAEILDEFLGADIVVIGAPMYNFTIPSQLKAWIDRILIA
                     GKTFGYSESGPEGLAGGKRVIVASARGGIYSEGPASAIDFQESYLKHVFGFIGITDVE
                     FVRAEGLNLGAEQREAAMAQAHGQIAGGMAIAA"
     gene            complement(11311..13593)
                     /locus_tag="VFK00_09700"
     CDS             complement(11311..13593)
                     /locus_tag="VFK00_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HET7593264.1"
                     /translation="MDDKRKPKVEPYKGPAGGWGSLKSVGSILAREKAPLRTARELMR
                     QNKADGFMCVSCAWAKPADAHPFEFCENGAKATAWELTSETTGPDFFAEHTLTELLGW
                     TDHELERHGRLTQPMRYDRATDTYMPCEWSEAFAAIGAELKKLDPKSTVFYASGRASL
                     ETSYMYALFARLYGHNNLPDSSNMCHETTGTALKKSVGSPVGTVTLDDFDKTDCVFFF
                     GQNVGSNSPRMLHSLQHLSQRGVPIITFNPLRERGLESFKNPQNPVQMLGGKATPISS
                     QYHQVKAGGDIAAIMGICKALFALDDESVAQNLPRVLDHEFIAQHTHGFEEFAVSVHS
                     HEWDAIERAAGLERAALEEAARVYAQSHAAIGIYGMGLTQHHFGEHNLHMIANLLLLR
                     GNVGKPGAGLCPVRGHSNVQGQRTVGIADDPKLVPLDRLAEQYGFEPPREPGMKTVQV
                     CEGVLKGTVHAFIGLGGNFLRAVPDSPRVEEAWSRLRLTVQIATKLNRSHLINGEVAY
                     LLPCLGRIDLDVQNGKPQVVSVEDSTSCIHGSFGKHKPISDALLSEPRIVAEMAKATL
                     ASNPNVPWDEWVNDYSKVRDAIEQTYPEQFKDFNKRMFSPGGFHRPNAARHRQWKTES
                     GKANFISPPTLSATGFEDAPDRLRLVTLRSNGQFNTTVYSNDDRFRGVHGTRMIVFMN
                     PADIAHFKLRDGQEITLVSDAGDDVHREVSGLRVVGYDIPQGCIGGYYPECNALIPYW
                     HHAQESKTPAAKSVPVRIVV"
BASE COUNT         2430 a         4644 c         4419 g         2182 t
ORIGIN      
        1 gtgggcgacc tgttcacgcg cggcgaagaa cagatcatca ccgcgctgcc gctgtaccac
       61 atcttctcgc tgacggtgaa cgcgcttttc atgacgtact tcggcggcga gaacgtgctc
      121 atcaccaatc cgcgcgacat acccgccttc atcaagacgc tgaagcaaaa ccgctggacc
      181 gcgataacag gcgtcaacac actgttcaac gcgctggtcc accatcccga cttcgcatcg
      241 atcgactttt cgcgggtgaa gttctccctg gccggcggca tggcggtgca gcgcgcggtg
      301 gcggagcgct ggaagaaagc gacaggcatc ccgctgatcg agggctacgg ccttacggaa
      361 acctcgccgg tggtcaccgc caatccgctg gacgtcaagg aatactcggg ctccatcggg
      421 atgccgctgc cgtccaccga cgtcgtgctg cgcgacgacg gccacgaggc gccgcccggc
      481 gagccggggg agctgtgcgt gcgggggccg caggtgatga agggctactg gaaccgcccc
      541 gaggaaacgg ccaacgtgat ggacggcgac ggctggttgc ggacggggga catcgcacgc
      601 atggacgaga agggtttcct gtacatcgtc gatcgcaaga aggacatgat cctcgtgtcc
      661 ggcttcaacg tgtatccgaa cgagatcgag gacgtggtcg tccgccatcc cggggtgctc
      721 gaggccgccg cgatcggcgt gccggacgaa cactcggggg aagtcgtgaa actgttcgtg
      781 gtgaagaagg atcccgcgct cacggaggcc gcgctcaagc agtattgcca cgacgagctg
      841 accggctaca agcggccgaa gtacatcgag ttccgcgacg agctgcccaa gagcaacgtc
      901 ggcaagatcc tgcgacgcga gttgcgcgac gagggaaaag agcgggaaca gggaacgggg
      961 aactgggaac agaaaagcag cccaccagca aggtagaaac tccagaccat gcccaggatc
     1021 gacatcgaag cgacgccccg ccacacgggt tgcggttacc cgccgccatt cgacgagcct
     1081 tgccgcccgc gcatccggca gcggctcgga gacgctggcg gactcacgga tttcggcgtg
     1141 aacctgctgg aactcgcgcc gggtgcgtgg tccagccaac gccattggca ttccgccgag
     1201 gacgaattcg tgtgggtgct ggacggcgag gtcacgctgg tcgacgacgg cggcgagcat
     1261 gtcctccacg ccggggaatg cgcggcattc ccgaagggcg atgccaatgg ccatcatctg
     1321 gtgaaccgtt ccggcgcgac ggtccgctgc ctcgagatcg gctcgcgcaa accgggcgcc
     1381 gacgcctgcg actatccgga cgtcgatctt cgcatcggtc ccggccgcga cgccggctat
     1441 acccataaag acggttcgcg gtattagcgc gaacccgagg ccggcattcc cccatatcgc
     1501 gtgcacgcgg cggacgtggc cgccctgcac catcacgccg accgatccca acgctgaatc
     1561 cgcgcccaac gcagcatgaa cgctgctcga ccgcgacggc gctgcgcgtc aactggcgcc
     1621 gcctcgtcgc gttcaaacgt ttgggccaac cccggcccga ccggaggatc accgtgaagc
     1681 gcattgctac aaccgtactg gttgcaggtc tcatggccac gccgatgctg gcgttgccgc
     1741 cgcccgcgaa ggcggcgatc agcgtcggca tttcggtcaa cctcgcgccg ccgccgctgc
     1801 ccgtgtacgg gcaaccggcc gttccggggc cgggctacat ctggacgccg ggcttctggg
     1861 cgtgggatcc cggattcggc tactactggg tgccgggcac gtgggtgatg ccgccccaga
     1921 tcgggctgct gtggaccccg ggctggtggg gctggtccga cggctactat cgctggcatc
     1981 ccggatactg gggccggcac gtcggcttct acggcggcat caattacggc cacgggtatt
     2041 tcggcgccgg ctacgtcggc ggcgagtggc gcggacgcga cttctactac aaccgcgccg
     2101 tgaacaacgt cagcgtggcc aacatccaca acgtgtacgt caaccgcacg gtggtgagga
     2161 acgtccatgt cgatcgcgtg agctacaacg gcggtcgcgg cggcatcacg gcgcatccca
     2221 cggccgcgca gcgcgcagtg gcgaacgcac gtcgctggtc gcccaccagc atgcaggagc
     2281 gccagcgcga ggtagcgaag ggcaacccgg gccagcgctt caaggcccac cagggccggc
     2341 ccgccgtatt cgccacgcaa catgccggca agttcgaggg tccgcatgcg gtgcacgcgc
     2401 cgtccgcacc gcgcgggtat gcaacgggtg cgtcgaacca tgcatcccgt gctgccccgc
     2461 agccacaccc ggccaacacg cccgatcgcg cacgcaccat ggtccaggca ccgcgcgaac
     2521 gtacccatcc cgccccgaac aatgtgccga accgcggaca cgcgcccgtc caggccccgc
     2581 gcgaacgggc acgcccggtg cagcagcagc cgatgcaacc gcagccctcg ttccggaacg
     2641 cacctgtcca cggcaacgca aggccgaacc cgtcgcaccc ggcgaccttg cagaacgcgc
     2701 cccgcaacgc accgccgcac aacggccaat cgagccgggg ccagccgcct gcatcgtcgt
     2761 cacacggcaa cgaccgtggc gggaagccgc aaaacgacaa gcacaagcac gatggcggtc
     2821 atcgaaactg acaatgtgcg gcacatgcga acggggcgac ccatggtcgc cccgtcgtcg
     2881 tccgaccgct gcccggctca gccgcgaatg gcgtcgagct cgtcgtccca ccgctccagc
     2941 gtgtcggcat agcccccgag caggttccac agcgacacct gcacttcttc cggaatccgc
     3001 gcgaacgcga gggcgacgtg cgccgcggta acccgcacga cttccgccat cacgtgcacg
     3061 ttgatgtcat cgctgaagag catgtccagc acccagacgt cgcccgagct gccgtgccac
     3121 tcggtcggcc gcgcaagacg cacgcccgtc gccgaaatgt cggcgaggtc ggtctgccag
     3181 acatcgccgc gacggctcat cagcaccgcc gtctcgcgca ccatgcgtgc atgccgcacg
     3241 tgatcgaccg tccgatccgt gttgtagtcc ccagccacct cgaacattcc cttggctccg
     3301 tatcgtgttc gccggcgcgc ggccggtccc ccattgaacg acgttctgcc gcctcatggc
     3361 aagcaaggaa tgggccaagc cggcggcaag gaccgctgga cttgcaaccg gttagccctt
     3421 aacctacgca cgtgacgccg gtgcaccttg gccggcaccc ggtaaccatg ttgccgcgac
     3481 ccggtgccag gggcatcggg cggatacggg atcgggggat ccttcgaccc gggatagcgc
     3541 gaaacctgcc cgacgccgtt ggtgagtcac cgatgcccgg ctcggcaagt ccagaggcca
     3601 gcctgagtcc gagccgatgc cgccgcgcgg gaatgcgcgg tcgcgggcgc gtgcacaccc
     3661 gaaccagcga cgcccggtcc gcgacagttg atgttgcacg atgagcgaat ttcgccaatc
     3721 cgaggattgc ggcaccgcgc gtgccgtgga tccgatccat acgagtgccg gatgagcacc
     3781 gaatcgcagt ccaccatgag ccccgcttcc gcgccggaag cgtccgctgc aaccgcggca
     3841 ccgcgccagg aagagatgcc gctggcggtc gtgctggggc agccggtcct gcggatcccg
     3901 caggaccttt acattccgcc ggacgcgctc gaggtgatcc tcgaggcgtt cgagggtccg
     3961 ctggacctgc tgctgtacct gatccgcagg cagaacctcg acatcctcga catccccgtc
     4021 gccgaaatca cccggcagta catggactac atcgacgtga tgcgcgagat gcggctggag
     4081 ctcgccgcgg aatacctggt gatggccgcg atcctcgcgg agatcaaatc ccggctgctg
     4141 ctgccgaggc cgccacagga agaaggcatc gaggaagatc cgcgcgccga gctggtgcgc
     4201 cgcctgcagg agtacgagcg gttcaagaag gcagccgagg acatcgacgc gatgccgcga
     4261 ctggaacgcg acctggagaa cacccgggcg ttcgtcccgg accacaaggt ggtgaaactg
     4321 ccgccgccgg tggagcttcg cgaaatgctg ctggcactgc gcgacgtgct gcaccgcgcg
     4381 gaactctacg ggcaccacgc ggtggaacgc gagccgctct cggtgcgcca gcggatgagc
     4441 gacgtactgc gcaagctcgg agatggcgcc ttccaccgct tcgagacgct gttcgaggcc
     4501 accgaaggcc gattgggcgt ggtggtgacg ttcctgtcgc tgctcgagct ggccaaggat
     4561 cgcctggtgg acatcatgca ggaggttccg ctggcgccga tctacctgaa ggcgcgcgcg
     4621 acggcggaaa cggcggattg accggcggaa aagagcatct atccgcaagc aagcgcaaag
     4681 aacgcaaatg caagcaatgc ctactgctgc tgatgtaacc agatgacagt tgatcgtttt
     4741 tcggaaggcc ttttttccga cgcggatcaa agccggtgaa cacggataag gggcttttgc
     4801 tcagcgaata tccgcgttca tccgcgtcag gagctctgcc tttgattttg gacaacgcgc
     4861 tcagcgaggc gtaagtggaa caggaaaagc tgaagaacat cgtggaggcc gccctgcttg
     4921 cggcgcacca accattgacc ctgccgatgc tggcggcgct gttccaggac accgatgcgg
     4981 tttcccacga ggatctcgcg cgggcggtcg agacgctcgg ccaggcctgc gaaggccgcg
     5041 gcatcgaact ccaggaagtg gcttcgggtt tccggtacca ggtgcgcgag gatgtgcatc
     5101 cgtgggtctc gcggttgtgg gccgagcgcc agaccaaata ctcgcgtgcc ctgctggaga
     5161 cgctggcgct gatcgcctac cgccagccga tcacccgcgg cgaaatcgag caggtgcgcg
     5221 gcgtcgccgt cgccaccagc atcatgcgca cgctggagga gcgcgaatgg gtgcgcgtcg
     5281 tcggctaccg cgacgtcccc ggcaagcccg cgctgttcgg caccacccgc cagttcctcg
     5341 attatttcaa cctcaagtcg ctggaccagc ttcctacgct cgcggagatc cgcgagatcg
     5401 ccgatcccga cccacagctc gcgctcgcga gcagccggga cgacgcaggc aacccgccgg
     5461 atgcgccgtc ggatccttcc gacggcaccg atgaacctgc cggcgccgat gcgcccgccg
     5521 atgccccgcg caccaccgag gacgccgcat gagttccgca ccacgttccc tgctgaccct
     5581 gaagcggtcg tctgcccatg aggccgatgg cgccctggaa gaacgcctgc acaaggtgct
     5641 cgccaacgcc ggcctggggt cgcgccgcat gctcgaggag cggatcggtg ccggggagat
     5701 cctggtcaac ggcagcgtcg cgacgaccgg ctcgagcgtg cgtgccggcg atcgcgtgac
     5761 cgtggaccgc aagcagttcg tcgtcgccac cgatacccac gagcgcgcgg cggtcctgat
     5821 gtaccacaag cccgagggcg agttgaccac gcgcgacgat cccgaaggcc gtcccacggt
     5881 gttcgagaac ctgccccacc tgaacggttc gcgctggatc tcggtaggtc gcctcgatgc
     5941 caataccacc ggcttgttgc tgctgaccac cgacggcgaa ctcgccaata cgttgatgca
     6001 cccgagccac gaactcgaac gcgaatacct gtgccgcgtg cacggcgaag ttccggacga
     6061 ggcgctcgaa aaactcaagg ccggggtgga actggacgat ggccccgccc gcttcgacga
     6121 aatccacgtc gtcaaccgcg gcggcagcca ctgctggttc cgcgtcgtga tgcacgaagg
     6181 ccgcaaccgc gaggtgcgcc ggctgtggga ttcgcaaggc ttcctcgtca gccgcctgaa
     6241 gcgcatccgc tacggcaacg tcgagttgcc gcgaggcctg cggcccggcc agaacgaaat
     6301 gctcgccgaa gacgccgtga ggtcgctgcg cgaactcgcg ggcgcgggcc ccgagcagcc
     6361 gacgctgacg ctgaaaccgg tgctccacca gcgccacacg ccgcgggggg tgaccgaggt
     6421 caggcccgcc gaccagacac cccacgcatg gaccggcgtc cgccacgacg aggcgcgcga
     6481 gctggcggcg ttcgaccggg tccggcccga tgatttcaat ccgcgcggca agcggcgccg
     6541 cggaccgccg cgcggcgacg tcaatggcaa tgtcctgaaa cccgagaagc cttccgggcg
     6601 caagcgcaag aaccggcgtg gcgtcgcgcc cggccaggag ctgccgtcgg tgcgcacctg
     6661 gttcgcgggg gacaagcgtc ccggcgaacg ccaggggcga agacccggtg gcggcaagcg
     6721 tgggccgggc ggcggccgcg gtcccggcgg cggcaagggc cgcaacgggc cccgtggctg
     6781 acgaaggggg ctccatgagc acgcgaccgg gcgtcaccat ctggcacaac ccgcgctgct
     6841 ccaagtcgcg cggaaccctc gagctgttgc aggggcgcgg catcgaaccc gcgatcgtcg
     6901 attaccagaa gacgccgccg tccacgtcgg aaatcgcgcg cgccctggaa ctcctcggca
     6961 tcgaaccgcg cgaccttatg cgcaagggcg aggcggccta cgcggaactc ggactcgaag
     7021 acccgaagtt cacccgcgag caattgatcg acgcgatggc gacccacccg atcctgatcg
     7081 agcgcccggt cgttttcgcg aacggcaagg ccgcgctggg gcgcccgccc gcaaacgtcc
     7141 tcgcgatcct gtaactcctc tcccgccggg agaggggttg ggactgaggg ttcggcacgg
     7201 caaacgcatt acacgcgccg ccgtttgttg ctccttgcgg gaccgaaccc tcatccggcg
     7261 ctgcgcgcga aagaaacggc ggcgttcccc gccccttctc ccgaagggcg aagttgtcac
     7321 gtcaattcga acgcgtcggc atccatccag gcgggaaact tctcgcggaa tgcggcgagg
     7381 gcatcgggat cgagcgcaac ggtcacggtt tgctcctgcg cgccgatgtc agccatcgtc
     7441 tggccgagga aatccagcac cgcgctgtcc cccgaatacg gcaactggtt gccatcggtg
     7501 ccgacccggt tgaccccggc gcaatacgcg aggttctcga tcgcacgtgc gcgcagcagc
     7561 atgcgccacg cgtgggcgcg cgccgccggc cagttggcaa cgaacaatgc aaggtcgtag
     7621 tcgaagccct gcccggcgtg gtgtcgattg cgcgagaaca ccggaaacct gaggtcgtag
     7681 cagaccagcg gacagatcct ccagcctcgc cactcgacca ccagccgctc gccaccggcg
     7741 gcatagcgtt tgtgttcgtc ggcgtaccgg aacaggtgcc gcttgtcgta gtgccgcagc
     7801 gcgccgtccg gcgtcacgaa cagcagccgg ttgaacactt tgtccgcagc gggcccctcg
     7861 cccgcccgca actgcatgct gcccgtgacg gcggcatcca gtgccgccgc ctgctccttc
     7921 agccaggcca cgctcggacc ctccatggtc tcggcatcat ggatggcttc gttggaaaaa
     7981 ccgctggtga aggtttccgg aagcaccacg aggtcggtgc gccccgcgag cggggcgatc
     8041 agcgacgcgt agtagtcgcg gttggccgcc gggtcatgcc agcgggtcgc accctggacg
     8101 agcgaaactc tcagtgcttg cattcagttc accgacaccg aagcaacctt cttgacgcgg
     8161 atgaacgcgg attaaggcgg atgaacatcg atcacaaggc tcccattcgc gttgatccgc
     8221 ttctaagccg cttccatctc acagcttgca caaacgctcc gccgctgccg acagcgtatc
     8281 gtcgttcttg gcgaaacaga atcgcacgat cttggcggcg ggcggcgcgt cgtagaacgc
     8341 cgacaccggg atcgcggcca cgccggcctc acgcaccatc cactcgcaaa aattgaggtc
     8401 gtcggtatcc cggatctccg aatagtcggc caactggaag taaccgccgc gcaccgccgg
     8461 cagcacgaac cgcgatcccg acagcaattc gcggaagcgg tcgcgcttgt cctggtagaa
     8521 cgccgccagc tcaccggggt gctgcgcgtc cttctccata acttcggcaa aagcgcactg
     8581 tgccgggtgg aacgtgcaga acgtaaggta ctggtgcacc ttgcggaact cggcagtcaa
     8641 cggtttcggc gccacgcaat acccgacctt ccagccggtg cagtgccagg tcttgccgaa
     8701 cgaggagatc acgaagctgc gttcggcaag ctcgggcttg cgcgaagcgc tcatgtgacg
     8761 cgcaccgtcg aacacgatgt gctcgtacac ttcgtcggaa atcaccagca acccgtggcg
     8821 cgtgacgatc tcggccaggg cgtccaggtc gtgctgctcg aagatggccc ccgatggatt
     8881 gtgcgggctg ttgaccatga tcatccgcgt cttcggcgtc agcgcgtcgc gcacccgctg
     8941 ccagtccacc gcgaaatccg gcagcgccag cggcacgtgc acggccttgc ccccggccag
     9001 cgcgatggcg ggctcgtagc agtcgtagca ggggtccagc acgatcacct cgtcgtccgc
     9061 gtgcaccgcg cacgcgatcg cggcgaagat cgcctcggtc gcgccggacg tgaccgtcac
     9121 ctcggtatcg gcactgatgc ggcggccgta caagcggtcc acctgcagcg caatctgctc
     9181 gcgcagcttc ggtatgccgc tcatcggtgc gtactggttc ctgccctcct ccatcgcgcg
     9241 ggtcagcgcc tcgcggaggt tccgcggcgt ctcgaaatcg ggaaagccct gcccgaggtt
     9301 caccgccttg tgttccagcg cgagctggct catcaccgtg aagatggtgg tgccgacctt
     9361 gggcaacttg gtttcgatct gcatgggcac cggcatccgc acaacggtat tcgttcgatg
     9421 ttagcacgca gaagtggcgg cgctgctgcg caagtccggg ggaaaccgct gcgtgggttc
     9481 cgacctacct gtggccattc gttggccgcg gggaggaagc ggcatggccg cttccgccgc
     9541 ccgccatcga cccgtcgcgt acgtcgcgcg cgaacgtctc ggccaggaaa tccaccagcg
     9601 cgcgcacggc cggcagcatg ccgcgccggc tcggatacac catgtgcagc gtgccctgcg
     9661 gcagacccca ctcgggcagc acgatgcgca gccggccgct gcgaagttca tccgcgcacg
     9721 aggattccgg cacccacgca atccctatgc cttgcgccgc ggcttcgata agcacccgga
     9781 agtcaccggt aatgaggcgc ggcttcaccg ccaccgcggt cttgctgcag gtgcgatcgt
     9841 agagatccca agcctggtcc ccttcgtgct cgaacatgct gagcaccggc aacgtggaca
     9901 acgcatgcgg cgacgccggt tccccgtgtt cggcaaggaa gccagggctc gcaaccagca
     9961 ccacgcgcag cgcgccgaag ctccgtgcca ccagcagcgc atcagtgtcc agcttctcgc
    10021 gcacacggat cgcgacatcg aagccctcgc cgatgatgtc gacgcggcgg ttgctcacga
    10081 acagccggac ctgcacctcc ggatgctcgg cgaggaaaac cggcaggtgc cgggcaagca
    10141 ggctttgcgc gaccgagacc gggcaactga tccgcacgac gccacgcggc tcccccgcga
    10201 gctcggcggc cgcttcggtc gccgcccgcg cctcggcgag catgccctgg caatgacggt
    10261 agaaacgctc gccagcttcg gtcaccgcga tcttgcgggt ggtccgctgc aacagccgga
    10321 caccgagttt gtcctcgagc tgggacaccc gcttgctgag ccgcgatttc ggcacgttca
    10381 aggcgcggct ggcggccgag aagccgccgt gctcgaccac cgcggcgaaa aaatagaaat
    10441 cgttgagatc cggcaacgac ccattggcgg cgggcacggc gttctccacg gcgaacgatg
    10501 cgttcggatt gcggcatctt atcgaagccg cgcctggagc ctatcgtttg cgggtcgccg
    10561 caccatgcgg ctccatccgg aagccatcat gaaactgctg cacatcgatt ccagcgtcct
    10621 cggcgaaaat tccgcttcgc ggtcgctgag cgcggcaatc gtcgcgcgcc tgcgcaggga
    10681 gcatcgcgac ctcgaggtcg cctaccgcga cctggcggcg cagacccttc cgcacttcac
    10741 gccggcgctg gcagacggac atccgtgcgt cagccgcaac gccgagatcc tggacgaatt
    10801 tctcggcgcc gacatcgtgg tcatcggtgc gccgatgtac aacttcacca ttcccagcca
    10861 gctcaaggcg tggatcgacc gcatcctgat cgcgggcaag acgttcggct attccgaaag
    10921 cggtccggaa ggcctcgccg gcggcaagcg cgtgatcgtc gcttcggcgc gcggcgggat
    10981 ctacagcgaa ggtccggcat ccgcgatcga tttccaggaa agctacctga agcacgtgtt
    11041 cggcttcatc ggcatcaccg acgtcgaatt tgtgcgcgcg gagggattga acctgggcgc
    11101 ggagcagcgc gaagctgcga tggcgcaagc ccacgggcag atcgcagggg gaatggcaat
    11161 cgcggcgtga atctgaaacc cctctcccgc cgggagaggg gttggggtga gggtccggca
    11221 ctacgaacta ctcgcacagg acgtgcttgc tcgcctcttc gcgggcgctg cgcgctcggc
    11281 ccaagcccgc ctctcgcgca gcgacatgga tcaaacgaca atccgcaccg gcaccgactt
    11341 cgcggccggc gtcttgctct cctgcgcatg gtgccagtac gggatcagcg cgttgcactc
    11401 ggggtagtag ccgccgatac agccctgcgg tatgtcgtag ccgaccacgc gcaggccgct
    11461 cacttcgcgg tggacgtcgt cgcccgcgtc cgacaccagc gtgatttcct gcccgtcgcg
    11521 cagcttgaaa tgcgcgatat cggccggatt catgaacacg atcatgcggg tgccgtgcac
    11581 gccgcggaac cggtcgtcgt tcgaatacac cgtggtgttg aactggccgt tggaccgcag
    11641 ggtcacgagt cgcaaccggt cgggcgcgtc ctcgaatccg gtcgcgctca aggtcggcgg
    11701 cgaaatgaaa ttggccttgc cgctctcggt cttccactgg cgatggcgtg cggcattggg
    11761 ccggtggaac ccgccggggc tgaacatgcg cttgttgaag tccttgaact gctcgggata
    11821 ggtctgctcg atcgcgtcgc gcactttcga ataatcgttg acccattcgt cccacggcac
    11881 gttcgggttc gatgccagcg tcgccttcgc catttccgcg acgatccgcg gttccgagag
    11941 caaggcgtcg ctgatcggct tgtgcttgcc gaacgagccg tggatgcaac tggtggaatc
    12001 ctcgacgctc accacttggg gcttgccgtt ctgcacatcc aggtcgatcc gtcccaggca
    12061 aggcagcagg taggcgacct cgccgttgat gaggtgcgaa cggttcaact tggtcgcgat
    12121 ctgcacggtc agccgcagcc tgctccaggc ctcttccacc cgcggactgt cggggaccgc
    12181 gcgcaggaag ttgccgccga gcccgatgaa cgcgtgcacc gtccctttca acacgccttc
    12241 gcaaacctgc acggtcttca tgcccggctc gcggggcggt tcgaaaccat actgctcggc
    12301 gagccggtcc agcggcacca gcttcggatc gtcggcgatg ccgaccgtac gctgcccctg
    12361 cacgttggaa tgcccgcgca ccgggcacag gccggcgccg ggcttgccga cgttgccgcg
    12421 caacagcagc aggttggcga tcatgtgcag gttgtgttcg ccgaaatggt gctgggtgag
    12481 tcccatgccg tagatgccga tcgcggcgtg cgactgtgcg tacacacgcg cggcctcttc
    12541 cagcgcggca cgttccagtc cggctgcccg ttcgatcgcg tcccactcgt gcgagtgaac
    12601 ggaaacggcg aattcctcga aaccatgcgt atgctgcgcg atgaactcat ggtccaatac
    12661 gcgcggcagg ttctgcgcga ctgattcgtc gtccaacgcg aacagcgcct tgcagatccc
    12721 catgatcgcc gcgatgtcgc caccggcttt cacctggtgg tattgcgagc tgatcggcgt
    12781 ggccttgccc cccagcatct gcaccgggtt ctggggattc ttgaagcttt cgagtccacg
    12841 ctcgcgcagc ggattgaagg tgatgatcgg cacgccgcgc tgcgaaagat gctgcagcga
    12901 atgcagcatc cgcggactgt ttgagccgac gttctggccg aagaagaaca cgcaatcggt
    12961 cttgtcgaag tcatcgagcg tcaccgtgcc gaccggcgaa cccacgctct tcttgagcgc
    13021 cgtgccggtg gtctcgtggc acatgttgga gctgtccggc agattgttgt ggccgtacag
    13081 acgcgcgaac agcgcgtaca tgtagctggt ttccagcgag gcccggccgg acgcgtagaa
    13141 caccgtggac ttcgggtcga gcttcttcag ctccgcaccg atggccgcga aagcctcgct
    13201 ccactcgcat ggcatgtacg tgtcggtggc gcggtcgtag cgcatcggct gcgtcagccg
    13261 cccatgccgc tccagttcgt ggtcggtcca gccgagcagc tccgtgagcg tgtgctcggc
    13321 gaagaaatcc ggtccggtcg tttcgctggt cagttcccag gcagtggcct tggcgccgtt
    13381 ctcgcagaac tcgaagggat gcgcgtcggc gggcttggcc cacgcgcagg acacgcacat
    13441 gaaaccgtcg gccttgttct gccgcatcag ctcgcgcgcg gtcctgagcg gtgccttctc
    13501 ccgcgccagg atgctgccga cggatttcag ggagccccat ccaccggcag gtcccttgta
    13561 gggttccact ttcggtttgc gcttgtcgtc catggcacac ctcgcgctgt tgacctgccc
    13621 acagtgtaaa gcgatcagcg gcgagtggcg gagggagtgg attgctcggc gcatc
//
LOCUS       DASSNH010000261         6000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_898229, whole genome shotgun sequence.
ACCESSION   DASSNH010000261 DASSNH010000000
VERSION     DASSNH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898229"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFK00_09705"
     CDS             complement(<1..274)
                     /locus_tag="VFK00_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7593265.1"
                     /translation="MFEAAPITATDKAGVYAELAQQAEGLLHGETNLVANAANFAALV
                     FYALPDINWCGFYFLDGGELVVGPFQGKPACIRIAMGRGVCGIAAET"
     gene            374..1096
                     /locus_tag="VFK00_09710"
     CDS             374..1096
                     /locus_tag="VFK00_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET7593266.1"
                     /translation="MPEPPSPAEADAGREPAEGARLDNFVDGAFAFAITLLIISGGSL
                     PRNVAALEHALRGIPAFAACFGQLAFFWFGHVRWRDTVRLIDGTSMWLSLLLVFFALI
                     FVFPLHLVFAGLFSSVTGGALSSDFTSAPGNAIGDTAALFVCYGLSFACMAGTLATLY
                     WHGVRVAARNGGPQPVSPRLRVVLWGYVAAVGLLSTLMALALPPNMGAVDGLPGFAYF
                     LLGLLGPIIHFYRKRLAKRLPT"
     gene            1093..1788
                     /gene="bioD"
                     /locus_tag="VFK00_09715"
     CDS             1093..1788
                     /gene="bioD"
                     /locus_tag="VFK00_09715"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET7593267.1"
                     /translation="MKATGVFIAGTDTGIGKTRAACTLLHALRAAGLRATGMKPVASG
                     CESTPEGLRNEDALALIEASDPKPDRYEDCNPFAFAPPISPHIAAAEVEREVTMPPIE
                     DAYDTIALATDAVVVEGVGGWLAPISDSLRANAIPRHLRLPVILVVGLKLGCLNHAQL
                     TARAIHADDCELIGWIGNRIDPDMARPKENLVTLNRAIAAPCLGVIAHGAAPLSAAAS
                     LRDAADALNALRR"
     gene            complement(1892..5401)
                     /locus_tag="VFK00_09720"
     CDS             complement(1892..5401)
                     /locus_tag="VFK00_09720"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7593268.1"
                     /translation="MSSMIRKAVLAGCAVLAGAGLAACSQSPSPPAAPGEPSTPASST
                     GATPAPAPATSNAVASTGANVEGTRLLRFPDVCGDRVVFTYAGDLWTASTQGGTATRL
                     TAGPGLEMAARFSPDCRQIAFSGQYGGDSQVYVVPAGGGEPKQLTFYPAMGPLPQRWG
                     FDNQVYGWTPDGKSVLFRSWRDSRNISDPRVYTVAVTGGLPSVLPMPQSGTARFSPDG
                     KQMVYSPKFRDFRDWDRYSGGWAQDLFIYDFASKSAKNITNDPNTDRDPVWIGKSIYF
                     LSDRGPHLNLYRYDTTDGKTTQLTNYQTDDARWASGDAAGQIVYEVTGTLHVYDTRNG
                     SDRALTIHVPSDLVASRASERSVKKYVEDYALSPNGKRAAFTARGDVFSVPLEHGITL
                     DLTHTPGAHEREVSWSPDGKRVAYISDQSGEEEVWVRDADGTNPVMLTKGVIGRLYNT
                     QWSPDGKHIAFVDSSNRIHVIGSSAGANDQVVAQDPGQSRRDYAWSPGGHYLAYSLTD
                     KDTQLSRLYVYDLASGKAVELGRASADASAPAFSPDGKYLFFVGDREWSPQFSSVELD
                     YATNRTTQILAYALRKDVDDPFAPRNDSAAAEDEDDKDAAGKDKDKGDDKRPKPASEV
                     NDKIDFDGIEQRLVRAPIDPDNIPWVAVTPKAILYATTGAPFLGREGAVPLKVHAWSF
                     KERKDSDVYTWKPEGGGGEGGGGAAPLALSPDGSTLLVHDGDDYKRIDLDASKPKPRD
                     VKLDGLFMRVDPKAEYAEIFNDVWRRYRDYFSVTNMNGYDWNAIRAKYEPLLQYVGDR
                     TDLNYVLGEMVAELSNSHTYIAGGDLGLPDKPHVGLLGADFKLDAASGRYQVADVFPG
                     ENDEPRYRSPLTEVGVDVKPGDYILAINGTALTADENPYRLLRIAPGQMVQLLVNSRP
                     STDGARTVLVKPIGSETDLHYYDWVRSNREYVAKASDGRIGYLHIPDMGVDGAREFIK
                     WYYPQMRKPGLIIDVRDNGGGFMSQTMIERLSRKLLAYNYTRGSSITGTYPGAVPLGH
                     LAALSNGTTASDGDIFSYMFKQAKLGPLIGTRTWGGVVGISGLGPLIDGGDVFVPQFA
                     AIVGLDGKYAVEGHGVEPDIVVKEDVAQQLAGKDPQLDKAIEVLEKEIQAHPLNLPPQ
                     PPGLDKAPPHMRPKAAASAAPAG"
BASE COUNT          925 a         1997 c         1991 g         1087 t
ORIGIN      
        1 gggtttcggc ggcgatcccg cacaccccgc gccccatggc gatgcggatg caggccggtt
       61 tgccctggaa aggccccacc accagttccc caccgtcgag gaaatagaac ccgcaccagt
      121 tgatgtcagg cagcgcgtag aagaccagcg ccgcgaaatt ggcggcattg gcgacgaggt
      181 tggtctcgcc gtgcagcagg ccttcggcct gctgggccag ctcggcatac acgccggctt
      241 tgtcagtggc ggtgatcggt gcagcttcga acatgttgac tccctcgcgc gagaagcgct
      301 ttgagttaag cggcgcgcag cccctgcgcg tccgcttgaa cgaacggatt gcgattccct
      361 ctattatcgc gcgatgccgg agccgccatc gccagccgaa gcggacgcgg gacgcgaacc
      421 tgcggagggc gcgcggctcg acaacttcgt cgacggcgcc ttcgcattcg cgatcaccct
      481 gctgatcatc agcggcggca gcctgccgcg gaacgtcgcg gccctcgaac acgcgctgcg
      541 cggcatcccg gccttcgccg cgtgcttcgg gcaattggcg ttcttctggt tcggccacgt
      601 gcgctggcgc gacaccgtgc ggctgatcga tggcaccagc atgtggctgt cgctgctgct
      661 ggtgttcttc gcgctgatct tcgtgttccc gttgcacctc gtgttcgcgg gcttgttcag
      721 cagcgtcacc ggcggggcgc tctcgtcgga cttcacgagc gcgccgggca acgccatcgg
      781 tgacaccgcg gcgctgttcg tctgctacgg cctgtcgttc gcctgcatgg caggcacgct
      841 ggcgacgctg tactggcacg gcgtccgcgt cgccgcacga aacggcggcc cccagccggt
      901 cagtccgcgc ctgcgggtgg tgctgtgggg ctatgttgcc gccgtcgggc tgctgtcgac
      961 gcttatggcg ctggcgctgc ccccgaacat gggcgccgtg gacggtcttc cgggattcgc
     1021 ctatttcctg ctcgggctgc ttggcccgat catccatttc tatcgcaagc ggctggcaaa
     1081 gaggcttcca acatgaaggc aacaggggtg ttcatcgccg gcaccgacac cggcatcggc
     1141 aagacgcgcg cggcctgcac gctgctgcac gcattgcgtg cagcaggatt gcgcgccacc
     1201 ggcatgaagc cggtcgcgag cggctgcgaa tccacgccgg aaggccttcg caacgaggat
     1261 gcgttggcgc tgatcgaggc cagtgatcca aaacccgatc gctacgagga ctgcaatccg
     1321 ttcgcgttcg cgccgccgat ctcgccgcac atcgccgccg ccgaggtcga gcgggaggtc
     1381 acgatgccgc cgatcgagga tgcctacgac accatcgccc tcgccaccga cgccgtggtg
     1441 gtcgaaggcg tgggcggctg gctggcaccg atctccgact cactgcgcgc caatgcgata
     1501 ccccggcacc tgcggctgcc ggtgatcctg gtggtgggac tcaagctggg ttgcctcaat
     1561 cacgcgcagc tcaccgcgcg tgcgatccat gccgacgatt gcgaactgat cggctggatc
     1621 ggcaaccgca tcgacccgga catggcacgt cccaaggaaa accttgtgac actgaaccgc
     1681 gcgatcgccg cgccatgcct gggcgtgatc gcgcatggcg ccgcaccgct ctccgcggcg
     1741 gcttccttgc gggatgccgc cgacgcgctc aacgccctgc gccgctgagc gtcagccagg
     1801 cagcgcgccg cccgttcggc gatccatggc gccgggccgc agccggcacc agcgaagaac
     1861 gcgcgccccg cgatgggacg cgcacgctgt atcagcccgc cggcgcggcg ctcgctgcgg
     1921 cctttggccg catgtgcggc ggcgccttgt ccaggccggg cggctgcggc ggcaggttca
     1981 gcggatgcgc ctggatctcc ttctcaagca cctcgatcgc cttgtcgagc tgcggatcct
     2041 tgcctgcaag ttgctgcgcc acgtcttcct tcaccacgat gtcaggttcg acgccgtggc
     2101 cctcgaccgc gtacttgccg tcgaggccga cgatggccgc gaactgcggc acgaacacgt
     2161 cgccgccgtc gatcaacggc ccgagcccgc tgatgccgac cacgccgccc caggtgcggg
     2221 tgccgatcaa tggccccagt ttcgcctgct tgaacatgta cgagaagatg tcgccgtccg
     2281 acgcggtggt gccgttgctg agcgccgcca gatggccaag cggcaccgca ccgggatagg
     2341 tgccggtgat cgaactgccg cgcgtgtagt tgtaggccag cagcttgcgt gacagccgtt
     2401 cgatcatcgt ctgcgacatg aagccgcccc cgttgtcgcg cacgtcgatg atcaggcccg
     2461 gcttgcgcat ttgcgggtag taccacttga tgaattcgcg cgcgccatcg acgcccatgt
     2521 ccgggatgtg caggtagccg atccggccat cgctggcctt ggcgacgtac tcgcgattgg
     2581 accgcaccca gtcgtagtag tgcaggtccg tctcgctgcc gatcggtttc accagcaccg
     2641 tgcgggcgcc atcggtgctc ggccgcgaat tgacaagtag ctgcaccatc tgtcccggcg
     2701 cgatcctgag cagccggtac ggattttcgt ccgcggtcag cgccgtgccg ttgatcgcga
     2761 ggatgtagtc gcccggcttc acgtccacgc cgacttcggt caacggcgaa cggtagcgcg
     2821 gctcgtcgtt ttccccgggg aagacatcgg cgacctggta acggccgctc gcggcatcca
     2881 gcttgaagtc cgcacccagc aggccgacgt gcggcttgtc cggcagcccc aggtcgccgc
     2941 cggcgatgta ggtgtgcgag ttggacaact cggcgaccat ttcgcccagc acgtagttga
     3001 ggtcggtgcg gtcgccgacg tactggagca acggctcgta cttggcgcgg atcgcattcc
     3061 agtcgtaacc gttcatgttg gtgacactga aatagtcgcg ataacgccgc cacacgtcgt
     3121 tgaagatctc ggcgtattcg gccttgggat cgacgcgcat gaacaggccg tccagcttca
     3181 cgtccctggg cttgggcttg gatgcgtcga ggtcgatccg cttgtagtcg tcgccgtcgt
     3241 gcaccagcag cgtcgaaccg tccggcgaca atgccagcgg ggccgcgccg ccaccgcctt
     3301 cgccaccgcc gccctcgggt ttccaggtgt agacgtcgct gtccttgcgt tccttgaacg
     3361 accacgcatg caccttcagc ggcaccgcgc cttcgcgccc gaggaagggc gcgccggtgg
     3421 tcgcgtacag gatcgccttg ggcgtgaccg cgacccaggg gatgttgtcg ggatcgatcg
     3481 gcgcgcgcac caggcgttgc tcgatgccgt cgaaatcgat cttgtcgttg acttcggaag
     3541 ccggcttcgg ccgcttgtcg tcacccttgt ccttgtcttt tccggctgcg tccttgtcgt
     3601 cctcgtcttc ggcggccgcg ctgtcattgc gcggcgcgaa cggatcgtcg acatccttgc
     3661 gcagggcgta ggcgaggatc tgggtggtgc ggttggtcgc gtaatccagc tccacgctgg
     3721 agaactgcgg cgaccactcg cgatcgccga cgaagaacag gtatttcccg tcgggcgaaa
     3781 aggccggcgc gctggcgtcg gcggacgcgc gcccgagttc gaccgccttg ccggaagcga
     3841 ggtcgtacac gtacagccgc gacaactggg tgtccttgtc ggtcaacgaa taggcgaggt
     3901 aatgcccgcc cggcgaccac gcataatcgc gccgcgattg gccgggatcc tgcgcgacca
     3961 cctggtcgtt cgcgccggcc gagctgccga tcacgtggat gcggttgctg gaatccacga
     4021 acgcgatgtg cttgccgtcg ggtgaccatt gcgtgttgta caggcggccg atcacgccct
     4081 tcgtcagcat caccgggttc gtgccgtcgg catcgcgcac ccacacctct tcctcgccgc
     4141 tctggtccga gatgtaggcg acgcgcttgc cgtcgggcga ccatgacact tcgcgttcgt
     4201 gcgcgcccgg ggtgtgggtg aggtcaagcg tgatgccgtg ttccagcggc acgctgaaga
     4261 catcgccgcg cgcggtgaac gcggcgcgct tgccgttcgg gctgagcgcg taatcctcga
     4321 cgtatttctt cacactgcgc tcggacgccc gcgaggcgac caggtcgctc ggcacgtgga
     4381 tggtcagcgc gcgatcgctg ccgttgcggg tgtcgtagac gtgcagcgtg ccggtgactt
     4441 cgtacacgat ctgcccggcc gcatcgccgc tggcccagcg cgcgtcgtcg gtttggtaat
     4501 tcgtcagttg cgtggtcttg ccgtccgtcg tgtcgtagcg atacaggttc aggtgtgggc
     4561 cacgatcgga caggaaatag atgctcttgc cgatccacac cgggtcgcga tcggtgttgg
     4621 gatcgttggt gatgttcttc gccgatttcg aggcgaagtc gtagatgaaa agatcctgcg
     4681 cccagccgcc cgagtagcgg tcccagtcgc ggaagtcgcg gaacttgggc gagtacacca
     4741 tctgcttgcc gtcgggcgag aagcgcgcgg tgcccgattg cggcatcggc agcacgctcg
     4801 gcagaccgcc cgtgacggcc acggtgtaca cgcgcggatc ggagatgttg cgcgagtcgc
     4861 gccacgagcg gaacaacacg gattttccgt ccggcgtcca gccatacacc tggttgtcga
     4921 aaccccagcg ctgtggcaac ggccccatcg cgggataaaa cgtcaactgt tttggctcgc
     4981 cgccgcccgc gggcaccacg tacacctggc tgtcgccgcc gtactggccg ctgaacgcga
     5041 tctgcctgca gtccggcgag aagcgcgcgg ccatttccag gcccggcccg gcggtcaggc
     5101 gcgtcgcggt gccgccttgc gtggaggcgg tccataaatc gcccgcgtat gtgaacacca
     5161 cgcgatcgcc gcacacgtcg gggaagcgca acaggcgcgt gccttcgaca ttggcgcccg
     5221 tggaggcgac ggcattcgaa gtcgcgggcg caggtgccgg cgtggcgccg gtggacgatg
     5281 caggcgtcga cggctcaccc ggcgcggcgg ggggcgacgg ggactgcgag caggcggcca
     5341 atcccgcgcc ggcaagcacc gcgcaacctg ccagaacggc cttgcgaatc atggatgaca
     5401 tgctgcgtcc ctcgtgtggt ggcatggacg gccctgctat accggcattc cgccccgcga
     5461 accactgcgg aaagtcacgg agtgtgccgc cttgcaagga gcttgatgaa gtcgccggca
     5521 ggttcttgcg gctgacttac tagggaatct ctgattttgc tcgtcattcc ggaatcgcga
     5581 agcgatatcc gggacccagc gactttgcat ttcagtacgt tgcgtgaaag tcactgggtc
     5641 ccggctttcg ccgggatgac gagtgacgga attgaccaga ggttccccag gcgcggcgac
     5701 gagtcatggc gggcgctatg gcgaggagcc gcaacaacgc cagacgcgaa aaagcctgcc
     5761 ccggacttga tccggggacg cggcgagttc gcaagatgct tgcggggcgg cacgctcggg
     5821 catgacttgc ggcacggatg gcacgtcgcg cccgatcacg gcatcattga tcgtccgttc
     5881 cctcccgcct tcgtgcgttc cccggagccc gaacttgacc caagcccaac ggttctacat
     5941 cacgatcgcc aatctcgcgc aggcgcgcgg cccggatccc gacctggcgt ttcagggatc
//
LOCUS       DASSNH010000262         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_537357, whole genome shotgun sequence.
ACCESSION   DASSNH010000262 DASSNH010000000
VERSION     DASSNH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537357"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2554
                     /locus_tag="VFK00_09725"
     CDS             17..2554
                     /locus_tag="VFK00_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7593269.1"
                     /translation="MASLLARQNPDAELQAAFRRHLPQRLRTLLRRARAQCRAGWDCN
                     VLRSLHDEIAQLAGTCGRYGMLETGERLLALESALAPALAAHDVPDAASNARIDALLD
                     SLRPHLQQVSTAPSPPAWTQDATPAAAKAPFPRCEVPPANYRVQLGLVAGEDEDAAPT
                     ADAPEVASPSPQVPLPEPARGPRVRIVNDDDPLVNELMVRLDQQGCDVALVERVEDLA
                     EQLRSAPPDLAVVAADARTPLEALTPVLRDARRDPAHRVRLLVLLREPDVELRLRALR
                     AGADRCLAMPAAPGDAVAAALELAMVDQELPYRILIVDDDAPQALFAQAILRRAGMET
                     RVLGESLAVLEELDRFQPDLLLLDLNMPDCDGFELTALVREREGYVNTPIVFLSGDQD
                     EDRQFAALDAGGDDFLMKPIRPAHLVAAVTNRVRRARAAASRSRRKRRRDATTGLHER
                     MQVLDALSEHLAAGGGYAAPGGLLAIEVQNAATLRTRLGLIGFDQLLTQIGEFIVRNA
                     RAGAMTARHGQAGFLIFAPARNERELLALAGELATAARDQRFGAQASAVRLAFAACGF
                     DPDTSESAIALAAVEHTLRAARVSPDGIAAHSRLASGTDTITQRIESALDNGAFDLAF
                     QPIIPIRGAAAPHYQALLRLRHDGREFLAAELVPAAIRAGTIGAVDAWVVERCIGILE
                     ERQQEGEPVCLFASQSLRGWQEGVRREQFAARLTEDIGTGVLVLEFRCEEAHASPDAW
                     AELARALQQAGARLSLAGIDAGAVAAGLLDDLPLDFVKLAPDVGDGDLERIVAAAHSR
                     QLRVIAPQVDAVARAERLQTAGVDLLQGNYFQPPVATLDQAFRAGGP"
     gene            2557..4863
                     /locus_tag="VFK00_09730"
     CDS             2557..4863
                     /locus_tag="VFK00_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7593270.1"
                     /translation="MASPRDGSLVSWSRVTGSLALVAAGAVAALWVAFPHVVVLWVLL
                     LVALILIWLVRTPPSERARAVTTMPLQDPVDVAMRTRLASAQAELSSLRGVQRELLAA
                     KVSAEAAMLAKSEFLASMSHEIRTPLNGILPLLDIVLGTDLSPSQREYLVTANASAKE
                     LLRIVDDILDYSKAEAGKLVLESVSINIRELVDSVKRLLEKPAEAKGLAMRSVVDPDV
                     RPVVRGDPMRIRQILTNLMSNAIKFTQRGGVSVRVSRRNETATQHELLFAVRDTGIGL
                     AADAAARLFQPFTQADASVTRRYGGTGLGLTICKKLVDLMGGRIGVRSEPGRGSVFWF
                     TIPFEKAPGDIAGPQQHIAGSRALLVASPQRAQHILPLLAALELDAAQVADTTSALNR
                     VRQAAPLGRAFGFDILIIDGESATGDAGKLLRSVLADRQLDSLRLMALGLDNAPDARV
                     VLAPSGATDAAIRQGVRSAFGLGAQARSQPVLTVPALPSGPALDGLPLAGKVLLVEDH
                     PVNQKVARKLLERLGLTVDVADNGELALDRLREQPYAMVLMDCQMPVLDGYSATRRLR
                     EIEAEQGKTRTPVIAMTAHAMSGDRERCLQAGMDDYLSKPLDRQLLEQTLSRWMQHAP
                     NTAPAMDDPVPQDPPAPPSKASAPAMHTASPPDTLDTATLVDLEDIMGDELATLVDAY
                     LRDGDTRMRNLHEAAARGDSTEVGKLAHSLKSSSANLGAMPLANRARQVEEAARSGTL
                     ANPVDSVAALEQLYANAAAALRERYKRP"
     gene            complement(5057..5527)
                     /gene="bfr"
                     /locus_tag="VFK00_09735"
     CDS             complement(5057..5527)
                     /gene="bfr"
                     /locus_tag="VFK00_09735"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET7593271.1"
                     /translation="MQGDAKVIEFLNRVLCNELTAINQYFVHYRMYDNWGFQELAEHE
                     RAESIDEMKHADRLIERILFLEGLPNLQKLNKLLVGQNPKEAIECDLKLERAARSDLR
                     EAIAHCESIGDYVSRTLFADIMHSEEEHIDWLETQLGLVEQVGLERYLQTHIRS"
     gene            complement(5701..5895)
                     /locus_tag="VFK00_09740"
     CDS             complement(5701..5895)
                     /locus_tag="VFK00_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7593272.1"
                     /translation="MYVCICKAVSDHDIRRAVADGARCFEDVQARTGCATCCGCCEPE
                     ARQVFEASLDAVQPALPVAA"
BASE COUNT          953 a         2110 c         2052 g          934 t
ORIGIN      
        1 gtagcgggga gtcctcatgg catcgctgtt ggcccggcag aatcccgatg cggaactgca
       61 ggcggcattt cgccgccatc ttccgcaacg cctgcgtacg ctgcttcggc gcgcgcgcgc
      121 gcagtgccgt gccggatggg attgcaacgt gctgcgatcg ctgcacgacg agatcgcgca
      181 gttggcgggc acctgcggcc gctacggcat gctcgagacg ggtgagcggt tgctggcgct
      241 ggaatcggcg ttggcgcccg cgctcgccgc gcacgatgtt ccggatgcgg ccagcaatgc
      301 caggatcgat gcattgctcg acagcctgcg cccgcatttg cagcaggtgt ccacggcgcc
      361 cagcccgccg gcatggacgc aggacgcgac gccggccgcc gccaaggctc cgttcccgcg
      421 ctgcgaagtg ccgccggcga attaccgcgt gcagctcggt ctcgtcgccg gcgaagacga
      481 agacgccgcg cccacggcgg acgcccccga agtcgccagc ccatcgccgc aggttccatt
      541 gcccgaaccc gcacgcggtc cgcgcgtgcg gatcgtcaac gacgacgatc cgctggtgaa
      601 tgaattgatg gtgcggctcg accagcaggg ctgcgacgtg gcgctggtcg agcgcgtgga
      661 ggacctggcc gagcagttgc gcagcgcgcc gcccgacctc gcggtggtcg ccgcggacgc
      721 acggacgccg ctcgaggcat tgacgccggt gctgcgcgat gcccgtcgcg atccggccca
      781 ccgcgtccgc ctgctggtgc tgttgcgcga acccgacgtc gaactccgct tgcgtgcgct
      841 gcgcgccggt gcagaccgct gcctcgcgat gccggcggcg cccggcgacg ccgtcgcggc
      901 ggcgctcgaa ctcgcgatgg tcgaccagga attgccctac cggatcctga tcgtcgacga
      961 cgacgcgccg caggccctgt tcgcccaggc gatcctgcgc agggccggca tggagacgcg
     1021 ggttctcggc gagtcgctgg cggtgctgga agaactggac cgtttccagc ccgacctgct
     1081 gctgctcgat ttgaacatgc cggactgcga cggtttcgag ctgaccgcac tggtgcggga
     1141 gcgggaaggc tacgtcaaca caccgatcgt gttcctgtcg ggcgaccagg acgaggaccg
     1201 ccagttcgcg gcgctggatg ccggcggcga cgatttcctg atgaagccga tccgtcccgc
     1261 acacctggtc gccgccgtca ccaaccgggt acggcgcgcg cgcgccgccg cctcgcgcag
     1321 ccggcgcaag cgccgccggg acgcgacgac cggcctccac gaacgcatgc aagtgctgga
     1381 tgcattgtcc gaacaccttg ccgccggggg cggctacgcg gcgcccggcg gcctgctcgc
     1441 gatcgaggtg caaaacgccg cgacgctgcg cacccgactc ggtctcatcg gcttcgacca
     1501 attgctgacg caaatcggcg aattcatcgt gcgcaatgcc cgcgccggcg cgatgaccgc
     1561 gcgccatggc caggccgggt tcctgatctt cgcgccggcg cgcaacgagc gggagctgct
     1621 ggcactggcc ggtgaactgg caaccgcagc ccgcgaccag cgcttcggcg cccaggcttc
     1681 ggcggtacgg ctggcattcg ccgcctgcgg cttcgacccg gataccagcg aatccgcgat
     1741 cgcgctggcg gcggtggaac acacgctgcg ggccgcgcgg gtgagccccg acggcatcgc
     1801 cgcacattcc aggcttgcat ccggcaccga cacgatcacg caacgcatcg aatcggcgct
     1861 ggacaatggt gcgttcgacc tcgcattcca gccgatcatc ccgatccgtg gagcggccgc
     1921 gccgcattac caggcgctgc tgcgcctgcg ccacgacggc cgcgagttcc tcgccgccga
     1981 actggttccg gcggcgatcc gcgccggaac gattggggcg gtggacgcat gggtagttga
     2041 gcgctgcatc ggcatcctcg aggaacggca gcaggagggc gagccggtat gtctgttcgc
     2101 cagccagtcg ctgcgcgggt ggcaggaggg cgtgcgccgc gaacagtttg ccgcccgcct
     2161 caccgaggac atcggaacgg gcgtgctggt actggagttc cgctgcgagg aagcgcacgc
     2221 cagtccggat gcctgggccg agctcgcacg cgcgctccag caggccggtg cgcgcctctc
     2281 gctggccggc atcgacgccg gagccgtcgc ggcggggctg ctcgatgacc tgccgctcga
     2341 cttcgtgaag cttgcgcccg acgttggcga tggcgacctc gagcgcatcg tggcggccgc
     2401 ccattcgcgc cagttgcgcg tcattgcccc gcaggtcgac gcggtcgcgc gcgccgagcg
     2461 gctgcaaacg gccggtgtcg acctgctgca gggcaactac ttccagcccc ccgttgccac
     2521 gctcgaccag gcattccgcg ccggaggacc ttgatcgtgg cgagtccgcg cgacggaagc
     2581 ctggtctcgt ggtcgcgcgt cacgggcagc ctcgcgctgg tcgccgccgg cgccgtcgct
     2641 gcgctgtggg tggcgtttcc gcacgttgtc gtgctctggg tgttgctgct ggtggcactg
     2701 atcctgatct ggctggtgcg gacgccgccc agcgaacgcg cgcgtgcagt gacgaccatg
     2761 ccgctgcagg acccggtgga cgtggcgatg cggacccgcc tcgcatccgc gcaggcggaa
     2821 ctgagttcgc tgcgtggcgt gcagcgcgaa ctgctcgcgg ccaaggtgtc cgcggaagcc
     2881 gcgatgctcg ccaagagcga attcctggcg agcatgagcc acgagatccg cacgccgctc
     2941 aacggcattc tgccgttgct ggacatcgtg ctcggcaccg acctttcgcc gagccaacgc
     3001 gaatacctgg tcaccgccaa cgcctcggcc aaggaattgc tgcggatcgt cgatgacatc
     3061 ctcgactact ccaaggccga agccggcaag ctcgtgctcg agtcggtgag catcaacatc
     3121 cgcgagctgg tcgacagcgt gaagcgcctg ctggaaaagc cggccgaagc caagggcctc
     3181 gcgatgcgct cggtggtgga cccggacgtc cggccggtgg tacgcggcga tccgatgcgg
     3241 atccgtcaga tcctgaccaa cctgatgagc aacgcgatca agttcaccca gcgcggcggc
     3301 gtttcggtgc gggtcagccg ccgcaacgag accgccaccc agcacgaact gctgttcgcc
     3361 gtgcgcgaca ccggcatcgg cctcgccgcc gacgcagcgg cgcgtttgtt ccagccgttc
     3421 acccaagccg acgcctcggt gacgcgacgc tacggcggca cgggcctggg cctcacgatc
     3481 tgcaagaaac tggtcgacct gatgggcggc cggatcgggg tgcgctcgga acccggccgc
     3541 ggctcggtgt tctggttcac gatcccgttc gagaaggcgc ccggcgacat cgccggcccg
     3601 caacagcaca tcgccgggtc acgcgccctg ctggttgcca gtccgcagcg cgcccagcac
     3661 atcctgccgc tgctggcagc cctggaactc gacgccgccc aggtcgccga cacgacgagt
     3721 gcgctcaacc gcgtccgcca ggccgcgccg ctgggccgcg cgttcggatt cgacatcctg
     3781 atcatcgacg gcgagagcgc gaccggcgat gccggcaagc tcctgcgcag cgtgctggcc
     3841 gaccgccagc tcgattcgct gcggctgatg gcgctcggat tggacaacgc acccgacgcc
     3901 agggtcgtgc tggcgccgtc gggcgccacg gacgcggcga tacgacaggg cgtgcgcagc
     3961 gcgttcgggc tcggcgccca ggcacggtcg cagccggtcc tgacggtgcc cgccttgcct
     4021 tcgggccccg ccctcgacgg gttgccgctc gccggcaagg tgttgctggt cgaggaccat
     4081 ccggtcaacc agaaggttgc ccggaaactc ctggagcgcc tcggcctgac ggtggatgtc
     4141 gccgacaatg gcgaactggc gctcgaccgc ctgcgggaac agccgtacgc catggtcctg
     4201 atggactgcc agatgccggt gctcgatggc tacagcgcga cccgcaggct gcgcgaaatc
     4261 gaggccgagc agggcaagac ccgtacgccc gtgatcgcga tgaccgcgca cgcgatgtcc
     4321 ggcgaccgcg aacgctgcct gcaggccgga atggacgact acctgtccaa gccgctggac
     4381 cgccagctcc tggaacagac gctgtcgcgc tggatgcagc atgcgccaaa caccgccccg
     4441 gccatggacg atcccgtgcc gcaagatccc cccgcgcccc catcgaaggc ttccgcaccc
     4501 gccatgcaca ccgcatcccc gcccgacacc ctggacaccg caacgctcgt cgacctcgaa
     4561 gacatcatgg gcgacgagtt ggcgacgctg gtggatgcct atttgcgcga cggcgatacc
     4621 cggatgcgca acctgcacga ggcggccgcg cgcggcgaca gcaccgaggt cggcaagctc
     4681 gcgcattcgc tgaagtcgtc gagcgcgaac ctcggcgcca tgcccctggc caatcgcgcc
     4741 cggcaagtgg aagaggccgc ccgcagcggc acgctcgcga acccggtgga cagcgtcgcc
     4801 gcgctggagc agctttacgc gaacgccgcg gcagcgctga gggaacgtta caagcggccg
     4861 tagcagcgaa cgctcgcctt ggcgtgcctt ccaggaaaca gacgcgtcat cccgaactgc
     4921 ccgtgacgtg ggcgcggtcc gactgcgcca tctgcgctcc cgtgctggtc cgcggacagt
     4981 ctccgcccaa ccccggttcg catggggcgg gcattgcccg ccgccgacgc attccggaat
     5041 gacgattcat cggacgctag ctgcggatat gcgtctgcag gtagcgttcc aggccaacct
     5101 gttcgaccag gccgagctgg gtttccagcc agtcgatgtg ttcttcttcg gaatgcatga
     5161 tgtcggcgaa cagcgtgcgg ctgacgtaat cgccgatcga ttcgcagtgt gcgatcgcct
     5221 cgcgcaggtc cgaccgcgcc gcgcgctcga gcttcaggtc gcactcgatc gcttccttcg
     5281 gattctggcc caccagcagc ttgttgagct tctgcaggtt cgggagtccc tcgaggaaca
     5341 ggatgcgctc gatcaaccgg tcggcatgct tcatctcgtc gatcgattcg gcgcgctcgt
     5401 gttcggcgag ttcctggaac ccccagttgt cgtacatccg gtaatgcacg aagtactggt
     5461 tgatcgcggt cagctcgttg cacaacacgc gattgaggaa ctcgatgacc ttggcatcgc
     5521 cttgcatggt gtactcccgg aatgaaacgg cactcgcgac tatagcgccg acgccacttc
     5581 aaggcggctg ttgtgcaaag tcggcccatg gaaggacgtt tcggtgccgt ttaccaagcg
     5641 gccacaaggc gaagagaaat ccagcggtgc cgcgcccggg gacgatcgca ggaatagttc
     5701 tcaggccgcg acgggcaggg ccggctgcac ggcgtcgagg ctcgcctcga acacttggcg
     5761 agcctcgggt tcgcagcagc cgcaacaggt ggcgcagccg gtccgcgcct gcacgtcttc
     5821 gaagcagcgc gcgccgtcgg caaccgcgcg gcggatgtcg tgatcggaga cggctttgca
     5881 gatgcagacg tacatggcga accctcgggg gcgccattgg aatacgaatg cgaatgattg
     5941 tcaagtagct ttgcgacgcc tggatctacg ccgccgccgg ccttccatcg ggcgtccccg
     6001 gcgtggtccc gagttcaacg gaacatgccg cttccaccag gggcgcgaa
//
LOCUS       DASSNH010000263         9497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_537475, whole genome shotgun sequence.
ACCESSION   DASSNH010000263 DASSNH010000000
VERSION     DASSNH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537475"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFK00_09745"
     CDS             complement(<1..417)
                     /locus_tag="VFK00_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593273.1"
                     /translation="MNMSEASLGRRISMDRMNEWPKWQRWSVWLTSWALYLGGMALLL
                     MFRSFVVQGLALAVLCVGLVLLTYSSKAVLNERMRKVDRWYVRVLLPTFIVYMVLMLN
                     VWPMYSRIEVPWLRIVVVLLPTLPLAVVVWAMMRYMA"
     gene            553..1950
                     /locus_tag="VFK00_09750"
     CDS             553..1950
                     /locus_tag="VFK00_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7593274.1"
                     /translation="MPDFEARLFRTLLPIAFTTLAGCAVFHPLPLASSRGPKRVEDIT
                     VPAASMPTPALRAYPFDPSNGLDVTEIAMLAVANDPHLIVQRDAAGVAHAQAYAAGLL
                     PDPQLNYEHDRPTGDYPPGTTDAYTAGLTFDLGNLITRSARVESARAGARQVDLDLLW
                     SEWQAIAQARTLFDRVYYQYQLVSRLEQEQSALAPMQAAIARALRSGDLTYDTASTGL
                     NAASDVANLLGDARRQLDSAEHDLHDLLGLDASVPLHLAGGPFAVDPDAREARRALDD
                     MTVRRPDLLALQAGYRAQDAKLRAAILAQFPAITVGFVKARDNSNISSEGLSIALSLP
                     LFDGNRGNIAIERATRRQLHDQYAARLLTDRNDVQRLVGDLRNDRTQRTALAAHAAQL
                     ALARKNARTNYAAGRLDWPTYLALRTQSLAADTALLALDQNTHETAIALDALVGDWPD
                     ASRALQKRRLANGPP"
     gene            1947..3044
                     /locus_tag="VFK00_09755"
     CDS             1947..3044
                     /locus_tag="VFK00_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7593275.1"
                     /translation="MNGKRFPFQPHALVIGGLLCLLAACSSGTPDAETQPAIAVGTAL
                     PKMQTFRANIAAFGQLTAGTGNALTLTLPQAGQIRAVHAIAGQRVQRGAALLELETDP
                     ATHSAYLQAQSALKLARDELTRTEHLRSEKLATNAQFDAARKALVDARATLDAQAKLG
                     GAQTTTILRAPAAGVVTALAVQRGERVAAGAALLQLSPTSAMEAQLGVDPGTAADINP
                     GMSITLVPVYAGRGAPPLHGTVAMVADAVDPQSHLVDVIATLDSPVSLPAGTALSGSI
                     VATPFKAWAVPRDALQNDDQGDFVYQIEHGKAKRVDVQVLAPNGSPIGVRGALDAQAP
                     VIALGSHEVSDGDPVRASAPASGASQGTAAR"
     gene            3041..6112
                     /locus_tag="VFK00_09760"
     CDS             3041..6112
                     /locus_tag="VFK00_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7593276.1"
                     /translation="MNIAAWFQAHRRSLLFLVLVLALGGLISVFSLPVALFPNVSFPR
                     IRVSIDAGDRPADQMAVAVTLPAAEAIRAVRGVRDVHSVTSRGSAEINVDFDWGADMG
                     RAYLDVNAAMSRILPELPAGARVEAVRMDPTVSEPVIAYSLRSKTLTQTQLHDLAKYQ
                     LVPLLSGVEGVAKVDVQGQGIGELHVDIDPGKLRAQDLSVADVEHAVASAADISGLGR
                     LADHYKLFLILANNQPGSVDALRDVVVRAGPQGVVRVGDVAAVTRDIKPEWVRTVADG
                     QPAILVQVFQQPSANSIQMVDAVKARLADYAPQMPRGVKIAAWYDQTQLVRGAVGSVR
                     DAILVGIVLAGVVLFLFLRNTRVILVALLVVPATLAITTLLLKIFGMSFNMMTLGGMA
                     AAVGLLIDDVVVMLEHIMRRIRETKGEIGTRITRAAREFAHPQAGSSLATIVIFLPLA
                     FLTGVTGAFFKALSLTMASALVISYLLTFVAVPLLAGRLIDERHVEERPPTRLWQRSI
                     TLYEKVLLRTLEWPALLLLGLIPLVVIGFLAWRSIGSGFMPRLDEGGFVIDYLSAPGT
                     SLEETNRLLEKVGAILHANPYVDTWSRRTGLQLGGGLTEANRGDFFVRLKDGSRPPTW
                     AVMEHIRQQAAASVPGLDVDVSQLIEDIIGDLTAVPEPIEVKLFSDNVTQLDAASKKV
                     AAQLRKIRGVVSVHDGINPAGDALEVHVDPVKAALLGLDPQSVAAQVNAAVAGTLAAE
                     LPQGPKMVGVRVWVPPDDRTEIEQLAALPISDGHGHVYPLSRVATLTQQRGQPEIDHQ
                     NLKRMVAVTARIEGRDLGSTMADVKQVLQTRGELPSGMYYELGGLYRQQQIAFHGLLL
                     VMATAVALVFTLLVFLYESFRIAIVILVQPLLAICAVFIGLWVTGIELNISAMMGMTM
                     IIGIVTELAIFYFSELAEVQAHGGDEQRSLRELLLEAGKRRSRAILMSAIAAILTLSP
                     LALAIGRGARMQQPLAIAIIFGMAAAVPLVLVVMPTLYSLLLRQGSDRSRT"
     gene            complement(6120..7514)
                     /locus_tag="VFK00_09765"
     CDS             complement(6120..7514)
                     /locus_tag="VFK00_09765"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HET7593277.1"
                     /translation="MKKKSFSLRARLTLLLALAVLVVFGAAAKIVDWRADAEMQQRFD
                     ASLLSRAQSLAALTRIENGRVAVDADNAAAAVFPGDAESSWYDLRCDGTVIARSAEAP
                     PSLAAQVEPVFADARSHDGRALRVVAFRFTPAQAGRAAPGVAPGCALTYALESGPLDE
                     LLDTLDIILLGCLLGACALVLLLTPWLVRRGLRPLSVLDRAMADIGPDAPGGRLPASD
                     TNELAPLVARFNEVLVRMDEGLARERQFASGLAHEFRTRLAELRALVDVERRYPSGRD
                     AHGLLGEIGSIGAELEATVTALLQLTRIQSGLEQSRPEHVPLGPQLARVCERHQDAAR
                     AREVRIEIAGSDDPDLAIDTDPTLLEIVLDNLLGNAVAYVPAGGVVRLHARRDGIDVC
                     NAAPALHADDLAKFGQRFWRKDAQGAGHAGLGLALAAAAARALRMRLSFALRDGVLCA
                     SVVWTRVTHDSWNG"
     gene            complement(7613..8278)
                     /locus_tag="VFK00_09770"
     CDS             complement(7613..8278)
                     /locus_tag="VFK00_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7593278.1"
                     /translation="MKLLLVEDSERLRATLARGLRAAGFTVDAAADGVEATGYLATYG
                     YDFVVLDLGLPRMDGFGVLRALASGGERPRVLVLSARDRVADRVDALNAGADDYLMKP
                     FAFEELVARLHALARRPAETHTDVLEHAGVRLDPQARRASANGSVLQLTPREFGLLEL
                     LLRHRGRVFPRADILERIAGSESEASDRSIEVVVFGLRRKLADAGCGGLIENRRGAGY
                     LIP"
     gene            8418..8957
                     /locus_tag="VFK00_09775"
     CDS             8418..8957
                     /locus_tag="VFK00_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET7593279.1"
                     /translation="MKRSTTIPLASALGLALALPALAGSIAHEDRMEMTAVMQAPITP
                     SHAIRIAENGGGTAYGYGMESTRNGRNWYVVDVLRGGAKLAVRIDPATGKVLGSSAAM
                     GEGAEGSNALAGSKLSFSEAVARAERVGHGPALEANAAGHGGNAHVDVDVVKGPNIAH
                     YRVSMRGGQIKTALTGTDG"
     gene            9094..>9497
                     /locus_tag="VFK00_09780"
     CDS             9094..>9497
                     /locus_tag="VFK00_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7593280.1"
                     /translation="MQSGTLLRRLAIWLLIGIAATAPTRAAFAASGPFGIDHRIAYDN
                     SGVWARHNQKVLMGATIVTVAGGSLALGDRSKLGDTFWRALDSMAVSSAVSQAAKWTF
                     QRERPSQTPNPDEFFKGIRYESFPSGEVTAMAG"
BASE COUNT         1655 a         3265 c         3070 g         1507 t
ORIGIN      
        1 cgccatgtac cgcatcatcg cccacaccac gacggccagc ggcagcgtcg gcagcagcac
       61 cacgacgatc ctcagccacg gcacctcgat acgggaatac atcggccaga cgttcaacat
      121 cagcaccatg tagacgatga aagtcggcaa cagcacgcgc acgtaccagc ggtccacctt
      181 gcgcatgcgc tcgttgagca cggccttgga cgaatacgtg agcagcacca ggcccacgca
      241 cagcaccgcc agcgcgaggc cctgcaccac gaagcttcgg aacatcagca gcaatgccat
      301 gccgccgaga tacagcgccc agctcgtcag ccagacgctc cagcgctgcc acttgggcca
      361 ctcgttcatc ctgtccatcg aaatccgtct ccccaggctc gcttcggaca tgttcatcgt
      421 gtcaagttga cttgacacac gaggttagag ggcgattcct atgttgtcaa gcttgcttga
      481 caacgcccac cgcacaatcc cacgaccgac gatcctaagc gccacctaag tgtgggctgg
      541 caccttgccg gcatgcccga tttcgaggcg cgcctgtttc gcacgctcct gccaatcgcc
      601 ttcacaacac tggccggctg cgccgtgttc catccgctgc cgctggcgag cagccgtgga
      661 ccaaagcggg tggaggacat cacggtgccc gccgcttcga tgccgacgcc tgcgctgcgt
      721 gcgtatccgt tcgatccgtc gaacggcctc gacgtcaccg agatcgcgat gctcgcggtc
      781 gccaatgatc cgcacctgat agtccagcgc gacgcagcgg gcgtggccca tgcgcaggcc
      841 tacgcggcgg gtctcctgcc ggacccgcag ctgaactacg agcacgatcg cccgacgggc
      901 gattatcccc cgggcaccac cgatgcctac acggccgggc tgacgttcga tctcggcaac
      961 ctgatcacgc gttcggcgcg ggtggaatcg gcacgggccg gcgctcgcca ggtcgatctc
     1021 gatctgttgt ggagcgaatg gcaggcgatc gcgcaggcgc gaaccctgtt cgatcgcgtg
     1081 tactaccaat accagttggt gagccggctt gagcaggagc agtccgcgct ggcaccgatg
     1141 caagccgcga tcgcccgcgc gttgcggtcg ggcgacctca cgtacgacac cgccagcacc
     1201 ggcctgaatg cggcgtccga cgtcgccaac ctgctcggcg atgcacgacg ccagctcgac
     1261 tcggccgagc acgatctcca cgacctgctg ggtctcgatg cttccgtgcc gttgcacctg
     1321 gccggcggcc cctttgcggt cgaccctgac gctcgggaag caaggcgggc actcgacgac
     1381 atgacggtgc ggcgccccga cctgctggcg ctgcaggccg gctaccgcgc gcaggatgca
     1441 aagctgcgcg ccgccatcct cgcgcaattc ccggcgatca ccgtcgggtt cgtgaaggcg
     1501 cgcgacaaca gcaacatctc cagcgagggt ctttcgatcg cgctttcgct gccgctgttc
     1561 gatggcaatc gcggcaacat cgcgatcgaa cgcgcgacgc gccggcaact gcacgaccaa
     1621 tatgcggcgc gtctgttgac cgatcgcaac gacgtgcagc gcctggtggg ggatctgcga
     1681 aacgaccgca cgcaacgcac ggcgttggct gcgcacgccg cgcaactggc actggcgcgc
     1741 aagaatgccc gcaccaatta tgcggccgga cgcctggact ggccgaccta cctcgcgctg
     1801 cgcacgcaat cgctggcagc cgacaccgcc ttgctggcgc tggaccagaa cacccacgaa
     1861 accgcgattg cgctggatgc gctggtgggc gactggcctg acgcatcgcg tgcgctccag
     1921 aagcggcgac tcgcgaacgg acccccatga acggaaaaag attccccttc cagccgcacg
     1981 cgctggtgat cggcggactg ctttgcctgc tggcggcttg ctcttccggc acaccggatg
     2041 ccgaaacgca acccgcgatc gcggtcggca ccgcgctgcc gaaaatgcag accttccgcg
     2101 cgaacatcgc ggcattcggc caactcaccg ccggaacagg caatgccctc acgttgacgc
     2161 tgccgcaagc ggggcagatc cgcgctgtcc atgcgatcgc cggccagcgc gtccaacgcg
     2221 gcgcggcctt gctggagctt gaaaccgatc ccgccacgca cagcgcctac cttcaggcac
     2281 aaagcgcatt gaaactcgca cgcgacgaac tcacccgcac cgaacacctg cgttcggaaa
     2341 aactcgcaac caatgcgcag ttcgatgccg cacgcaaggc actggtcgat gcgcgggcga
     2401 cgctggatgc acaggcgaag ctcggcggcg cacaaacgac aaccatcttg cgtgcgccgg
     2461 ccgccggcgt cgtcaccgcg ctggcggtgc agcgcggcga gcgggtggcc gccggcgccg
     2521 ccttgctgca gctctcgccg acctcggcga tggaggcgca actcggtgtc gatccgggca
     2581 cggccgccga catcaacccc ggcatgtcca tcacgctcgt gccggtctac gccggccgcg
     2641 gcgcaccacc gttgcacggc accgtcgcga tggtagccga tgcggtggac ccgcaatcgc
     2701 acctcgttga cgtgatcgcg accctcgatt cgccggtgtc gcttccggcc ggcaccgcgt
     2761 tgtccggaag catcgtcgcc acgccattca aggcttgggc ggtcccgcgc gatgccctgc
     2821 aaaacgacga ccagggcgac ttcgtttatc agatcgaaca cggcaaggcc aagcgggtcg
     2881 acgtgcaggt gctcgcgccg aacggcagcc cgatcggcgt ccggggcgcg ctggacgcgc
     2941 aggcgccggt gatcgcgctc ggcagccatg aagtttccga tggcgacccg gtgcgcgcgt
     3001 cggcgccggc gtccggcgcg tcgcagggaa ccgcggcgcg atgaatatcg ccgcatggtt
     3061 tcaggcacat cgccgttctt tgctgttcct ggtcctggtc ctcgcgctgg gcggactgat
     3121 atccgtcttc agcttgccgg tggcgctgtt cccgaacgta tcgttcccgc gcatccgcgt
     3181 cagcatcgat gccggcgatc gccccgccga ccagatggcg gtggcggtga cactgcctgc
     3241 cgcggaagcg atccgcgcgg tgcgcggcgt gcgcgatgtc cattccgtca ccagccgtgg
     3301 cagcgccgag atcaacgtgg acttcgattg gggcgcggac atgggccggg cttatctcga
     3361 cgtgaatgcc gcgatgagcc ggattcttcc ggagttgccg gccggtgcgc gcgtcgaggc
     3421 ggtgcggatg gacccgaccg tttccgagcc cgtcatcgcc tacagcctgc gcagcaagac
     3481 gctgacgcag acccaactcc acgatctcgc gaagtaccag ctggtcccgc tgctcagcgg
     3541 cgtcgagggc gtcgcgaagg tggacgtgca agggcaagga atcggcgaac tgcatgtcga
     3601 catcgatccc gggaagctgc gcgcgcagga tctctccgtc gccgacgttg agcacgcagt
     3661 cgccagcgca gccgacatct cggggttggg cagactggcc gatcactaca agctgttcct
     3721 gatcctcgcc aacaaccagc ccggcagcgt cgatgcattg cgcgacgtgg tcgtgcgcgc
     3781 cgggccccaa ggcgtggtgc gggtgggcga cgtcgccgcc gtcacgcgcg acatcaagcc
     3841 cgagtgggtg cgcaccgtcg cggatggcca gcccgcgatc ctggtgcagg tgttccagca
     3901 accgtccgcc aacagcatcc agatggtcga cgcggtgaag gcgaggctgg ccgattacgc
     3961 cccgcagatg ccccgcggag tgaagatcgc ggcctggtac gaccagaccc aactggtgcg
     4021 aggcgcggtc ggcagcgtgc gcgacgcgat cctggtcggc atcgtgctcg ccggcgtggt
     4081 gctgttcctg ttcctgcgca acacgcgggt gatccttgtc gcgctgctgg tggtgccggc
     4141 gacgctcgcg atcaccactt tgctgctgaa gatcttcggg atgagcttca acatgatgac
     4201 gctgggcggg atggcggccg cggtcggatt gttgatcgac gacgtggtgg tgatgctgga
     4261 acacatcatg cgccgcatcc gcgaaacaaa gggtgagatc ggcacgcgca tcacccgggc
     4321 ggcgcgcgag ttcgcgcatc cgcaggccgg ttcgtcgctc gcgaccatcg tgatcttcct
     4381 gccgctggcg ttcctgaccg gggtgaccgg tgcattcttc aaggcgctgt cgctgacgat
     4441 ggcgagtgcg ctggtgattt cgtatctcct caccttcgtc gcggtgcccc ttctggctgg
     4501 gcgcctgatc gatgagcggc acgtggagga gcgcccgccg acgcggctgt ggcagcgctc
     4561 gatcacgctt tacgagaagg tgcttttgcg caccctcgaa tggccggcgc tgctgctgct
     4621 tggcctcatc ccgctggtgg tcatcggctt tctcgcttgg agatcgattg gcagcggctt
     4681 catgccaagg ctcgacgagg gcggcttcgt gatcgattac ctctccgcgc cgggcacttc
     4741 gctcgaagaa accaaccggc tgctggagaa ggtgggcgcg atcctgcacg cgaaccccta
     4801 cgtggacacc tggtcgcgcc gcaccggcct gcaactcggc ggcggcctca cggaagccaa
     4861 ccgtggcgac ttcttcgtgc gcctgaagga tggctcgcgg ccgccgacgt gggcggtgat
     4921 ggaacacatt cgtcaacagg ctgccgccag cgttccgggg ctggacgtcg atgtctcgca
     4981 attgatcgag gacattattg gcgacctaac cgcggtgccg gagcccatcg aggtgaaatt
     5041 gttctcggac aacgtgacgc agctggatgc cgcatcgaag aaggtcgccg cgcaactccg
     5101 gaagatacgg ggcgtggtca gcgtgcacga tggcatcaat cctgcgggcg acgcgctgga
     5161 ggtgcacgtc gacccggtga aggcggcgtt gctgggactc gacccgcaaa gcgtcgccgc
     5221 ccaggtcaat gccgcagtgg cgggcacgct ggcggccgag ctgccgcagg gaccgaagat
     5281 ggtgggcgtg cgcgtgtggg tgccaccgga tgaccgcacc gaaatcgaac aactcgccgc
     5341 gctgcccatc agcgatggcc acggccacgt gtatccgctg tcgcgcgtcg cgacgctgac
     5401 gcaacaacgc ggccagcccg agatcgacca ccagaacctg aagcgcatgg tggcggtgac
     5461 cgcgcgcatc gaggggcgcg acctcggatc gacgatggcc gacgtcaagc aggtgctgca
     5521 gacccgcggc gagttgccgt ccggaatgta ttacgagctg ggtggcttgt accggcagca
     5581 acagatcgcg ttccacgggt tgctgctcgt gatggcgacg gcggtcgcgc tggtgttcac
     5641 gctgctggtg ttcctgtacg aaagcttccg catcgcgatc gtgatcctgg tccagccctt
     5701 gctggcgatc tgcgcggtgt tcatcggcct gtgggtgacc ggcatcgaac tcaacatctc
     5761 ggccatgatg ggcatgacga tgatcatcgg aatcgtcacc gaactcgcga ttttctattt
     5821 ttccgaactc gccgaggtcc aggcccatgg cggcgacgaa caacgctccc tgcgcgaact
     5881 gctgctggaa gccggcaagc ggcgcagccg cgcgatcctg atgtcggcga tcgccgccat
     5941 attgacgctg tcgccgctgg cgctggcgat cgggcgtggt gcgcggatgc agcagccgct
     6001 ggcgatcgcg atcatcttcg ggatggccgc agccgtgccg ctggtgctgg tggtgatgcc
     6061 taccctgtat tcgctgctgc tgcggcaggg atccgaccga tcacgaacgt gagcggcggc
     6121 tacccgttcc atgaatcgtg tgtcaccctg gtccagacca ccgacgcaca cagcacgcca
     6181 tcgcgcaatg caaacgacaa cctcatccgc agcgccctgg cggcggccgc ggccaaagcc
     6241 agaccaagac ccgcgtgacc cgcgccctgc gcatccttgc gccagaagcg ctgaccgaat
     6301 ttggcgagat cgtcggcgtg cagcgcaggc gccgcgttgc atacgtcgat tccgtcccgc
     6361 ctcgcgtgca gtctgacaac gccgccggcc gggacgtaag ccacggcgtt gccgagcagg
     6421 ttgtcgagca cgatttcgag cagcgtcgga tcggtgtcta tcgccagatc cggatcgtcc
     6481 gatcccgcga tttcgatgcg cacctcgcgc gcacgcgcag catcctggtg tcgctcgcac
     6541 acacgcgcga gctgcggccc cagcggcacg tgttcgggac gtgattgctc cagccccgac
     6601 tggatgcgcg tcagttgcag cagcgcagtg acggtcgcct ccagctccgc accgatgctg
     6661 ccgatctcgc cgagcaggcc atgcgcgtcg cgtccactcg gatagcggcg ttccacgtcc
     6721 accagcgcgc gcaattcggc gagccgcgta cggaattcgt gcgcaaggcc cgaggcgaac
     6781 tggcgttcgc gagccagccc ttcgtccatc cgcaccagca cctcgttgaa gcgcgcgacc
     6841 aacggggcca gctcgttcgt gtcgcttgcc ggcaggcgac cgccgggtgc atccgggccg
     6901 atgtcggcca tcgcgcgatc cagcaccgag agtgggcgca ggccacgccg gacaagccac
     6961 ggcgtcagca gcagcaccag tgcgcaggcg cccagcaggc aacccagcag gatgatgtcg
     7021 agtgtatcca gaagctcatc cagcggtccg ctctcgagtg cataggtgag cgcgcagccc
     7081 ggtgcgacgc ccggggcggc ccgcccggcc tgcgctggcg tgaaccggaa ggcgaccacg
     7141 cgcagcgcgc ggccatcatg cgagcgcgca tccgcgaaca cgggctcgac ctgggcggcg
     7201 agagagggcg gcgcttccgc gctgcgcgcg atcaccgtgc catcgcagcg caggtcgtac
     7261 caactggatt ccgcgtcacc tggaaacacc gcggcggccg cgttgtcggc gtccacggcg
     7321 acgcggccat tctcgatccg cgtcagcgcc gccaacgatt gcgcgcgcga caacaacgaa
     7381 gcatcgaagc gctgttgcat ctcggcgtcg gcgcgccagt ccacgatttt cgctgccgcg
     7441 ccgaacacca ccagcacggc cagcgccagc agcagcgtca ggcgcgcacg cagcgagaag
     7501 gatttctttt tcatcgcgat actcgatgct cgcttgccct catccaatcc tgcgccccct
     7561 tctcccgcgt gcgggagaag ggaaccctgc gcggcgtttc gccgcaccac gttcatggaa
     7621 tcagatatcc ggcgccgcgc cggttttcga tcaggccacc gcagccggcg tcggcgagct
     7681 tacgccgcag gccgaacacg accacctcga tgcttcggtc cgacgcttcg ctttcgctgc
     7741 cggcaatccg ctccaggatg tcggcgcgcg gaaacacccg gccgcgatgg cgcagcagca
     7801 attcaagcaa cccgaactcg cgcggcgtga gttgcagcac gctgccgttc gcgctggctc
     7861 gacgcgcctg cggatcgagc cgcacacccg cgtgttccag cacgtccgta tgcgtctcgg
     7921 cgggacgtcg cgccagcgcg tgcaggcgcg ctaccaattc ctcgaacgcg aacggtttca
     7981 tcaggtaatc gtcggcaccg gcattcagcg catccacgcg atcggcgacc cggtcacgcg
     8041 ccgacaacac cagcacgcgt ggccgttccc cgcccgaagc cagcgcgcgc aacacgccga
     8101 acccgtccat tcgcggcagc ccgagatcca gcaccacgaa atcgtaaccg taggtcgcaa
     8161 gatagccggt ggcttcgacg ccgtcggccg cggcatccac cgtgaatccc gcggcgcgca
     8221 acccgcgcgc cagcgttgcg cgcaaacgtt cggaatcttc gaccagcaac agcttcatgc
     8281 gcgtcgtccg cgtggcggtg ggctaaaagc tatcaccctg aacacgcccc caccaccaac
     8341 gacacccaaa gctcggcaaa gcaatggcta aggcggcggg ttcagtctgg gttttccccg
     8401 caaaaggagt gtccgtgatg aaacgaagca ccacaatccc gctcgcatcc gctctgggcc
     8461 tggcgctggc cctgccggcg ctcgccggca gcatcgccca cgaagaccgg atggagatga
     8521 ccgcggtgat gcaggctccg attacgccga gccacgccat caggatcgcc gaaaatggtg
     8581 ggggaaccgc ttacggctac ggcatggaat cgacccggaa cggccggaac tggtatgtcg
     8641 tggacgtgct gcgagggggc gcgaaattgg ccgtgcgcat cgaccccgcc accggcaagg
     8701 tgctcggcag cagcgccgcc atgggcgaag gcgctgaagg ttcgaatgcg ctggcgggca
     8761 gcaagctgag cttcagcgaa gccgtcgccc gagccgaacg agtcggacat ggccccgcac
     8821 tggaagccaa cgctgcaggt cacggcggca atgcccacgt cgacgtggac gtcgtcaagg
     8881 gcccaaacat cgcgcattac cgcgtgtcca tgcggggagg tcagatcaag accgcattga
     8941 ccgggacgga cggctgagcc cgccaccgca agcttcgggc gccgaggaca ccccgcccgc
     9001 ggctgccttg caaggttccc gaaagccacg gcgaagcggc cgctaagcaa gcggctgcaa
     9061 agtgtcgcca tcgcaaaacc gacgatcatc ggcatgcaat caggcaccct gctgcgacgc
     9121 ttggcgatat ggctgctgat cggcattgca gccaccgcgc cgacgcgggc agcctttgcc
     9181 gcgagcggtc cgttcggcat cgatcaccgc atcgcctacg acaacagcgg agtctgggct
     9241 cgccacaacc agaaggtgtt gatgggcgcc acgatcgtga ccgtggcggg cggctcgctc
     9301 gcactcggcg accggtccaa gctgggtgac acgttctggc gcgcgctgga ttccatggca
     9361 gtgtcgtctg cggtctcgca agccgccaaa tggactttcc agcgcgaacg gccgtcgcag
     9421 acgccgaatc cggacgagtt cttcaagggc attcgctacg agagctttcc gagcggcgag
     9481 gtcaccgcga tggccgg
//
LOCUS       DASSNH010000264         6489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_55057, whole genome shotgun sequence.
ACCESSION   DASSNH010000264 DASSNH010000000
VERSION     DASSNH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55057"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFK00_09785"
     CDS             <1..1060
                     /locus_tag="VFK00_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HET7593281.1"
                     /translation="AIGSGVRLTGVTQQFGNGDIETTQNSLDLAISGNGFFTTKGTNG
                     LAYTRAGDFQQDKSGYVVTPEGNRLQVFPPNGAGGFDISTMQDLQLVTTQSKAQATGN
                     VQIAFNLPADATTPTTTPFDPADPTSFNQGTPFTVYDSQGGTHNATAYYVKTAPNQWD
                     VHLMIDGQDAGTNSMTFSNTGSLQIPANGQLGFNSVVVSPGADPVQLTMDMSKATQVG
                     SAFSTGAVDQDGFPAGQLSNIDISADGVVSARFSNGQSTPLGQLALANFANLQGLRQL
                     GNTSWAESYESGQAVRGTAGSGKLGGIQSGALEASNTADLTSQLVNMIKEQRNYQANA
                     QVISTDNKLSQTIMSITS"
     gene            1085..1825
                     /gene="flgF"
                     /locus_tag="VFK00_09790"
     CDS             1085..1825
                     /gene="flgF"
                     /locus_tag="VFK00_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HET7593282.1"
                     /translation="MDRSLYVAMTGATQILRAQTEVSHNIANASTTGFKAELSAFHAL
                     PVLGDGMPTRINAVDRGNGYDFTPGNQSTTGRDLDVAVQGQGWIAVQSPNGGEGYTRA
                     GNLQLTADGMLTDAGGNPVLGDGGPITLPPASHIDVGADGTVSVVPLGQTPDTLVNVG
                     RIRLVNPQVDQLEQGGDGLMHLRDGSQAPADATVQLASGVLEGSNVNPSTALVKMIEL
                     SRQFEMQIRAIHSADENAQAASQLLQAS"
     gene            1906..2691
                     /gene="flgG"
                     /locus_tag="VFK00_09795"
     CDS             1906..2691
                     /gene="flgG"
                     /locus_tag="VFK00_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HET7593283.1"
                     /translation="MFSALWVAKTGLDAQQMRMEVISNNLANVNTTGFKSSRASFQDL
                     VYQNVRQPGGQTTEQTESPSGLMLGTGVRVVGTEKLFTQGNLSQTGNALDVAIQGRGF
                     LQVSMPDGSVSYTRDGSLHMDQNGQLVTANGYPLNPSITIPPNAQAITIGTDGTVSVT
                     VPGQSAPTQVGTLQMADFVNPAGLQPTGDNLYVETVSSGSPQLGQPGLNGMGSLNQGS
                     LETSNVNVVEALVNMIETQRAYEMNSKAISATDSMLQYINNKV"
     gene            2697..3374
                     /gene="flgH"
                     /locus_tag="VFK00_09800"
     CDS             2697..3374
                     /gene="flgH"
                     /locus_tag="VFK00_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HET7593284.1"
                     /translation="MNPRFARLALLLPALAALAACATTAPHIDPAFAPTLPIAQAAPP
                     PADGSIYHEAGSMELFADPRAHRVGDILTIVLAENTQASKKAQTTTSKKDDVDIGAAT
                     LWGKSFPLNGGKIASVGGERGFDGSGSSSQSNQLNGQITVTVAERLSNGNLVIRGEKW
                     LTINQGRELVRIEGIARPQDISPDNTILSTRIANAQITYTGSGTLADANSQGWLSRFF
                     NSKWMPF"
     gene            3374..4549
                     /locus_tag="VFK00_09805"
     CDS             3374..4549
                     /locus_tag="VFK00_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET7593285.1"
                     /translation="MSTHVPARRIPVARIGRSNARSRWRACFATLLLAAIAALSLPLH
                     AERIGDLAQVAGVRSNQLIGYGLVVGLEGSGDQTSQAPFTTQSLENMLQQFGVTVPPN
                     VRPQLRNAAAVVVTASLPAFSKPGQAIDVTVASIGNAKSLRGGQLLMTPLRGADGNTY
                     AIAQGSVLVGGINAEGQSGSSVQVNISSTGRIPNGASVERTVPSSFASAGSLTLNLNT
                     PDFTTANRVAKAINDQFGAGTAEAHDAVSVQVRSPQDPTQRVGWIASIQNLDVTPGEA
                     PARVIVNSRTGTVVIGSDVKVSAAAVAHGSIEVSIGEQPLVSQPAPFSQGQTALVPSS
                     NVKIAEHGGHMFKFGPGVSLDQIVHAVNQVGASPNDLIAILQALKQSGALHAELVVI"
     gene            4549..5640
                     /gene="flgJ"
                     /locus_tag="VFK00_09810"
     CDS             4549..5640
                     /gene="flgJ"
                     /locus_tag="VFK00_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HET7593286.1"
                     /translation="MDPAASAPALHGPDTWTDIQGLAGLRRAAGQDGGKAALPAVARQ
                     FEAVFTQMLIKSMRDASFGGGILDSAQSDQWRDLFDKQIALSLANDGKGMGIAAMLVR
                     QLGGKPASTTAAAADPTALPAEARATRTLAGLPIGIPAPAIAAGGPMAGLVNSTASSI
                     GNAADAAGGFLDRALQFAKQAGSSALHAARDWAFSGPDDFVQKLAPYAQAVAQKLGVS
                     VRAVLAQAALETGWGKHMPRQADGSSSYNLFGIKAGGSWDGQRANVPTIEFEGGAAVR
                     RNAAFRAYQGPVQSLADYARLLTGSPRYQGALGHGEDIAGFARALCRAGYATDPGYAD
                     KLTAIADSDTMRDALASLKDPQSQPTTEL"
     gene            5671..>6489
                     /gene="flgK"
                     /locus_tag="VFK00_09815"
     CDS             5671..>6489
                     /gene="flgK"
                     /locus_tag="VFK00_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HET7593287.1"
                     /translation="MADLLGTGISGLLAARVALDTTGHNIANASVPGYSRQSVTLATR
                     EPERAGSYFIGQGVDVVGVQRAYSQYLTTALWNQDSSLQRAATFNQLTQDLNNLLGGS
                     NNLQSALDGFYASVQDAANDPASASTRQAMLGKAGSLAGTFHTLDQQLGQQSAQVNQR
                     ISDSVSAINSLSQSIASLNQQIQSQASGGTPPSDLLDRRDQLVQQLSEQVGVTVSAQG
                     NSINVFTGNGQTLVSGSTALALKAAPDPYDPTRVNVVSQTGATLDGQLQGGNLGG"
BASE COUNT         1198 a         2366 c         2031 g          894 t
ORIGIN      
        1 cgccatcggc agcggcgtgc gcctgaccgg cgtcacccag caattcggca acggcgacat
       61 cgagaccacc cagaacagcc tcgacctcgc gatcagcggc aacggcttct tcacgaccaa
      121 gggcaccaac ggcctggcct acacccgggc gggtgacttc cagcaggaca agtctgggta
      181 cgtggtcacc ccggaaggca atcgcctgca ggtgttcccg cccaatggcg ccggcggctt
      241 cgacatcagc accatgcagg acctgcaact ggtcaccacg cagagcaagg cccaggcgac
      301 cgggaacgtg cagatcgcct tcaacctgcc ggccgatgcg accacgccga ccaccacgcc
      361 gttcgatccc gccgacccga ccagcttcaa ccagggcacg cccttcacgg tgtacgactc
      421 gcagggcggc acccacaacg cgaccgcgta ttacgtcaag accgcgccca accaatggga
      481 cgtgcacctg atgatcgacg gccaggacgc gggcaccaac tcgatgacgt tttccaatac
      541 aggttccctg cagatccccg ccaacggcca gttgggcttc aactcggtgg tcgtgagccc
      601 gggcgcggat cctgtccagt tgaccatgga catgtccaag gccacccagg tcggcagcgc
      661 gttctcgacc ggcgcggtcg accaggacgg cttccccgcc ggccagttgt ccaacatcga
      721 catctccgcc gacggcgtgg tgtcggcgcg cttctccaac ggccagtcga cgccgctggg
      781 gcaactggcg ctcgcgaact tcgccaacct ccagggcctt cggcagctcg gcaacaccag
      841 ttgggcggaa agctacgagt cgggccaggc cgtgcgcggc acggccggca gcggcaagtt
      901 gggcggcatc cagtccggtg cgctcgaggc ttccaacacc gcggacctca cctcgcagct
      961 cgtcaacatg atcaaggagc agcgcaacta ccaggccaat gcgcaggtga tttccaccga
     1021 caacaagctc agccagacca tcatgagcat cacctcgtaa gtcgcacggg actgaggact
     1081 tcccatggac cgctcactct atgtagccat gaccggcgcc acgcagatcc tgcgcgcgca
     1141 gaccgaagtc agccacaaca tcgccaacgc ctcgaccacc gggttcaagg cggagctgag
     1201 cgccttccat gcgctgccgg tgctgggcga cggcatgccg acccgcatca acgcggtcga
     1261 ccgcggcaac ggctacgact tcacgccggg caaccagagc accaccgggc gcgatctcga
     1321 cgtcgccgtg cagggccagg gctggatcgc ggtgcaatcg ccgaacggcg gcgaaggcta
     1381 cacgcgcgcc gggaatttgc agctcactgc ggatggaatg cttaccgacg ccggcggcaa
     1441 tccggtgttg ggcgacggcg gcccgatcac cctgccaccg gcctcgcaca tcgacgtcgg
     1501 cgcggacggc acggtatcgg tggtaccgct gggccagacg ccggacaccc tggtcaatgt
     1561 cggtcgcatc cggctggtga acccgcaggt cgatcaactc gaacagggcg gcgacggact
     1621 gatgcacctg cgcgacggca gccaggcgcc ggccgacgcc accgtacagc tcgcctccgg
     1681 cgtgctcgag ggcagcaacg tcaatccctc cacggccctg gtgaagatga tcgaattgtc
     1741 gcgccagttc gaaatgcaga tccgcgcgat ccacagcgcg gacgaaaacg cacaggccgc
     1801 cagccaactc ctgcaggcaa gctgacatga caaacgcatg ccggttccgg ccattcacgc
     1861 tccaaccatt cgcaacctgc cgcgtcgcgg caaagggata gtccgatgtt ttccgcactg
     1921 tgggtcgcca agaccggatt ggatgcgcag cagatgcgca tggaggtcat ctccaacaac
     1981 ctggccaacg tcaataccac cggcttcaag agttcgcgcg catcgttcca ggacctcgtc
     2041 taccagaacg tgcgccagcc cggcggccag accaccgaac agaccgaatc gccctcgggc
     2101 ctgatgctgg gcaccggcgt gcgcgtggtc ggcaccgaaa agctgttcac ccagggcaac
     2161 ctcagccaga ccggcaacgc gctggacgtc gccatccagg ggcgcggctt cctgcaggtg
     2221 tcgatgccgg acggctccgt ctcctatacc cgcgacggtt cgctgcacat ggaccagaac
     2281 gggcaactcg tcaccgccaa cggttatccg ctgaatccgt cgatcacgat cccgcccaac
     2341 gcgcaggcga tcacgatcgg caccgacggc accgtcagcg tcaccgtgcc cggccaatcc
     2401 gcgccgacgc aagtcgggac gctgcagatg gcggacttcg tcaatcccgc cggcctgcaa
     2461 ccgaccggcg acaacctgta cgtcgaaacc gtctccagcg gctcgccgca gctcggccag
     2521 cccggcttga acgggatggg cagtctcaac cagggctcgc tcgaaacctc caacgtcaac
     2581 gtggtggagg cgctggtcaa catgatcgaa acccagcgcg cctacgagat gaactcgaag
     2641 gcgatctccg cgaccgattc gatgctgcag tacatcaaca acaaggtgtg atcgcaatga
     2701 acccccgctt cgcccgcctc gccctgctgc tgcccgcgct cgccgcgctg gcggcctgcg
     2761 cgaccaccgc gccacacatc gatcccgcgt tcgcgccgac cctgccgatc gcgcaggcgg
     2821 cgccgccccc ggccgatggc tcgatctacc acgaggcggg atcgatggaa ctgttcgccg
     2881 atccgcgcgc gcatcgcgtg ggcgacatcc tgaccatcgt gctcgccgag aacacccagg
     2941 ccagcaagaa ggcccagacc acgacctcca agaaggacga cgtcgacatc ggcgccgcca
     3001 ccttgtgggg caagagtttc ccgctcaacg gcggcaagat cgccagcgtc ggcggcgagc
     3061 gcggcttcga cggctcgggc tccagcagcc agagcaacca gttgaacggg cagatcaccg
     3121 tgaccgtcgc ggagcgcctg tccaacggca acctcgtcat ccgcggcgag aagtggctga
     3181 ccatcaacca gggccgcgag ctggtgcgga tcgaaggcat cgcgcggccg caggacatca
     3241 gccccgacaa caccatcctg tcgacccgca ttgccaacgc gcagatcacc tataccggca
     3301 gcggcacgct ggccgatgcg aattcgcagg gttggctatc gcgcttcttc aattccaagt
     3361 ggatgccctt ctgatgagca cgcacgttcc cgcccgccgc atccccgtcg cacgtatcgg
     3421 gcgcagcaac gcccgcagtc gctggcgcgc ctgcttcgcc accctgctgc tggccgcgat
     3481 cgccgcgctg tcgctgccgt tgcacgccga gcgaatcggg gacctggccc aggtggccgg
     3541 tgtccgcagc aaccagttga tcggttatgg gctggtggtg ggcctggaag gcagcggcga
     3601 ccagaccagc caggcgccct tcaccaccca gagcctcgag aacatgttgc agcagttcgg
     3661 cgtgacggtg ccgccgaacg tgcggccgca gttgcgcaat gccgccgcgg tggtcgtcac
     3721 ggcgtccctg cccgcttttt ccaagcccgg ccaggcaatc gacgtcacgg tcgcctcgat
     3781 cggcaacgcc aagagtttgc gcggcggcca gctattgatg acgccgctgc gtggcgccga
     3841 cggcaatacc tatgcgatcg cccagggcag cgtgctggtc ggcggcatca acgccgaggg
     3901 ccagagcggt tccagcgtgc aggtgaacat ctcgtcgacc gggcgcattc ccaacggtgc
     3961 gagcgtggag cgcacggtac cgtccagctt cgccagcgcc ggcagcctga cgctgaacct
     4021 caacacaccc gacttcacca ccgccaaccg ggtggccaag gccatcaacg accagttcgg
     4081 cgcaggcacc gccgaggcac acgacgcggt gtcggtgcag gtgcgctcgc cgcaggatcc
     4141 gacccagcgc gtcggctgga tcgccagcat ccagaacctc gacgtcaccc ccggcgaagc
     4201 gcccgcgcgc gtgatcgtga attcgcgcac cggcaccgtg gtgatcgggt cggacgtcaa
     4261 ggtcagcgcc gccgccgtcg cgcacggttc gatcgaagtc agcatcggcg agcaaccgct
     4321 ggtcagccag ccggcgccct tcagccaggg acagaccgcg ctcgtgccga gcagcaacgt
     4381 caagatcgcg gaacacggcg gccacatgtt caagttcgga cccggcgtca gcctcgacca
     4441 gatcgtgcat gcggtgaacc aggtcggcgc ctcccccaac gacctcatcg cgatcctgca
     4501 ggcgctcaag caatcgggcg cgctgcacgc ggaactggtg gtgatctgat ggacccggcg
     4561 gcgtccgcgc ccgcgctgca cggccccgac acctggacgg acatccaggg tctggccggc
     4621 ctgcgccgtg ccgcgggcca ggacggcggc aaggccgcgc tgcctgccgt ggcacggcag
     4681 ttcgaagcgg tcttcacgca gatgttgatc aagtcgatgc gcgacgccag tttcggcggc
     4741 ggcatcctcg acagcgcgca atccgaccag tggcgcgacc tgttcgacaa gcagatcgcg
     4801 ctgtcgctcg ccaacgacgg caagggcatg ggcatcgcag cgatgctggt gcgccagctc
     4861 ggcggcaagc cggccagcac caccgcggcc gctgcggatc cgaccgcgct gccggccgag
     4921 gcacgcgcca cgcgcacgct cgcgggcctg ccgatcggca tcccggcgcc agcgatcgcg
     4981 gcaggcggac cgatggcggg ccttgtcaat tccaccgcgt cgtccatcgg caacgccgcg
     5041 gacgccgccg ggggtttcct cgaccgcgcg ctgcagttcg cgaagcaggc cggcagcagt
     5101 gctctccacg cggcgcgcga ctgggcgttc tccggaccgg acgatttcgt gcagaaactc
     5161 gcgccctacg cgcaggccgt ggcccagaag ctgggtgtca gcgtgcgcgc ggtgctggcc
     5221 caggccgcac tcgaaaccgg ctggggcaag cacatgccca ggcaggccga cggcagcagc
     5281 agctacaacc tgttcggcat caaggccggc ggcagttggg acgggcagcg cgccaacgtg
     5341 cccaccatcg aattcgaggg tggcgcggcg gtgcgccgca acgccgcctt ccgcgcgtac
     5401 caggggccgg tgcagtcgct cgcggactat gcgcggctcc tcaccggaag cccccgctac
     5461 cagggcgcgc tcggccatgg cgaggacatc gccggcttcg cgcgtgcact gtgccgggcc
     5521 ggctatgcca cggaccccgg ttacgcagac aagctcacgg ccatcgccga cagcgacacc
     5581 atgcgcgatg cgctggcctc gctcaaggat ccccaatcgc agccgacaac ggagctgtaa
     5641 cgatgcgcat ccctaccagg agtacccgtc atggctgacc tgctgggcac cggcatctcg
     5701 ggcctgctgg cggcgcgcgt ggcgctcgac accaccggcc acaatatcgc caacgccagc
     5761 gtccccggct acagccggca atcggtgacg ctcgcgacac gcgagccgga acgcgcgggc
     5821 agctacttca tcggccaggg cgtcgacgtg gtcggcgtgc agcgcgccta cagccagtac
     5881 ctgaccacgg cgctgtggaa ccaggactcg tcgttgcaac gggccgccac cttcaaccag
     5941 ttgacccagg acctcaacaa cctcctgggc ggcagcaaca acctgcagag tgcgctggac
     6001 ggcttctatg ccagcgtcca ggacgccgcg aacgaccccg cttcggcctc cacgcgccag
     6061 gcgatgctgg gcaaggccgg ttcgctggcc ggcaccttcc ataccctcga ccagcaactg
     6121 ggccagcaat ccgcccaggt caaccagcgg atctccgaca gcgtctcggc catcaacagc
     6181 ctctcgcaga gcatcgccag cctcaaccag cagatccaga gccaggcctc cggcggaacg
     6241 ccgccgagcg acctgctcga tcgccgcgac cagctcgtgc aacagctttc ggagcaggtc
     6301 ggcgtcaccg tctccgcgca aggcaacagc atcaacgtct tcacgggcaa tggccagacc
     6361 ctggtcagcg gctcgaccgc gctcgccctg aaagccgcgc ccgatcccta cgaccccacc
     6421 cgggtgaacg tggtgtcgca gaccggcgcc accctcgatg gccagttgca gggcggcaac
     6481 ctgggcggc
//
LOCUS       DASSNH010000265         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_72643, whole genome shotgun sequence.
ACCESSION   DASSNH010000265 DASSNH010000000
VERSION     DASSNH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72643"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFK00_09820"
     CDS             complement(<1..789)
                     /locus_tag="VFK00_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7593288.1"
                     /translation="MTTFQHVMPFGAQVRDDAVRFRLWAPAQQAVSLHLESSGNGRDL
                     PMARDDDGWFELVTGDARAGDRYRYVLENGTRVPDPASRFQPDDVHGASEVIDPHAHA
                     WRHPEWHGRPWHEAVVYEAHVGTFSASGDYDGVREGLGHLADLGVTALELMPLSDFDG
                     RRNWGYDGVLPFAPDSSYGRPEALKRLIDEAHGLGLMVLLDVVYNHFGPSGNWLGAYA
                     PQFFTDRHHTPWGQAIDFGVGEVRRFFIDNALYWLEEYRFDGLRL"
     gene            complement(1036..>2049)
                     /locus_tag="VFK00_09825"
     CDS             complement(1036..>2049)
                     /locus_tag="VFK00_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010382022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HET7593289.1"
                     /translation="DAVAQDPLLARTKLIAEPWDVGPGGYQVGRFGANWSEWNDRYRD
                     SVRRFWRGDAGALPELATRITGSSDLFDHDGRNPRATVNFVTAHDGFTLRDLVSYEHK
                     HNEANQEDNRDGREDGLSANYGAEGETDDPAILELRRRQRRNMLATLLLSQGTPMLVA
                     GDEFGRTQHGNNNAYAQDNETSWIDWEAIDEAADGDIRFVRRLIGLRRNHPALRWPRF
                     MHGWVQCEKGIKDITWLAPEGGEMNEGHWSDPERRSVGLLLNGRADSRFGELPLDEIL
                     LLLLNAHPEDIDFHLPALPFGSGWVELLAPARRPESTLHEFDSTFRFAGRSVVLFHLA
                     VEV"
BASE COUNT          320 a          670 c          690 g          369 t
ORIGIN      
        1 gagccgcagt ccgtcgaagc ggtattcctc cagccagtac agcgcgttgt cgatgaagaa
       61 ccggcgcact tcgccgacgc cgaaatcgat ggcttggccc cagggcgtgt ggtgccggtc
      121 ggtaaagaac tgcggcgcgt acgcgccaag ccagtttccg ctcgggccga agtggttgta
      181 gacgacatcc agcagcacca tcagcccaag tccgtgcgct tcatcgatca gccgcttcag
      241 cgcttcgggg cggccgtagc tcgaatcggg cgcgaacggc aacacgccgt cgtagcccca
      301 gttgcggcgg ccgtcgaagt ccgacagcgg catcagctcg agggcggtga cgccgagatc
      361 ggcgagatga cccagccctt cgcgcacccc gtcgtagtcg ccgctcgcgg aaaacgtgcc
      421 gacgtgcgct tcgtaaacca ccgcttcgtg ccagggtcgg ccgtgccatt cggggtgccg
      481 ccacgcgtgg gcatgtggat cgatcacctc gctcgcaccg tgcacgtcgt ccggctggaa
      541 ccgtgaagcc gggtcgggga ctcgggtgcc gttttcgagg acataccggt agcggtcgcc
      601 ggcccgcgcg tcgccggtga cgagttcgaa ccacccgtcg tcgtcgcgcg ccatcggcag
      661 gtcgcgtccg ttgccggagg attcgaggtg caggctcacc gcttgctgcg cgggtgccca
      721 cagtcggaag cgcaccgcgt cgtcgcgcac ctgggcgccg aagggcatta cgtgctggaa
      781 tgtcgtcatc cttgaaccat tgtgtccatg cggtgccgca tcacccggcg cggctgtacc
      841 aatcgagccg gatcactcaa gaaatgtcgt catttccggg gccatcgtcg gcttcatcgg
      901 cgatggaacc cggaatccag ctccttgcgt acgggtggca cacagctgga ttccgggctc
      961 tgcccgcgac gacgcgggca gctcccacat gacgaatcct ttttccctcg tgcgcaaatg
     1021 agcaacaccg tggttctata cctccaccgc cagatggaac aggaccaccg agcggccggc
     1081 gaaacggaac gtggaatcga attcgtgcag ggtggattcg gggcgccgcg cgggggcgag
     1141 cagttcgacc cagccggagc cgaagggcag cgcgggcagg tggaaatcga tgtcctccgg
     1201 gtgcgcgttc aacagcagca acaggatctc gtccaacgga agttcgccga agcggctgtc
     1261 ggctcgaccg ttcagcagca gtccgacgct gcgtcgttcg ggatcgctcc agtggccttc
     1321 gttcatctcg ccgccttcgg gggcgagcca ggtgatgtcc ttgatgccct tttcgcattg
     1381 cacccagccg tgcatgaaac gcggccatcg cagcgcggga tggttgcggc gcaggccgat
     1441 caggcgtcgc acgaaacgga tgtcgccgtc ggcggcctcg tcgatcgcct cccagtcgat
     1501 ccagctcgtc tcgttgtcct gcgcgtacgc gttgttgttg ccgtgctggg tgcgtccgaa
     1561 ttcgtcgccg gccacgagca tcggcgtgcc ctgcgacaac agcagggtgg ccagcatgtt
     1621 gcggcgctgc cggcggcgca attcgaggat cgccggatcg tcggtttcgc cctcggcgcc
     1681 gtaattggcg ctgagtccgt cctcgcgtcc gtcgcggttg tcctcctggt tggcttcgtt
     1741 gtgcttgtgc tcgtaggaca ccaggtcgcg caacgtgaag ccgtcgtgcg cggtgacgaa
     1801 attgacggtc gcccgcgggt tcctgccgtc gtggtcgaac aggtcggacg aacccgtgat
     1861 gcgcgtggcg agttccggca gcgcgccggc gtcgccgcgc cagaagcggc gcacgctgtc
     1921 gcggtagcga tcgttccatt cggaccagtt ggcgccgaag cggccgacct ggtagccgcc
     1981 cggtccgacg tcccagggtt ccgcgatcag cttggtccgt gcgagcaacg gatcctgtgc
     2041 gaccgcgtc
//
LOCUS       DASSNH010000266         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1079948, whole genome shotgun sequence.
ACCESSION   DASSNH010000266 DASSNH010000000
VERSION     DASSNH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079948"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFK00_09830"
     CDS             complement(<1..756)
                     /locus_tag="VFK00_09830"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HET7593290.1"
                     /translation="MIEVAAGILVDDRGRVLLMQRLPGKHLAGLWEFPGGKVEPGETI
                     DEALVRELNEELGIAVLASAPLISLPWRYPEKAVRLHALRVTAWRGEPRAREGHPLRW
                     SALPDIDVSTMPPADAPIVTALHLPPCYAIVASGALLAPALVAHGAVPERVLWQLRMP
                     EAGRDEIRRFASDALATNPEVRGSLLINRDVELARELGVGVHLKASQLRHLVERPLPL
                     GSWVGASCHDAEELERAAELNADFATLSPVCATA"
     gene            complement(753..1238)
                     /locus_tag="VFK00_09835"
     CDS             complement(753..1238)
                     /locus_tag="VFK00_09835"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7593291.1"
                     /translation="MSISLIAALDRNRAIGRAGAMPWHLPDDLKRFKALTLGKPVLMG
                     RKTALAIGRPLPGRPNLVLTRADTAPFAGQSVVHSLDEAVAHAGDSDLCVIGGGEVYA
                     LALPCATRLHLTEIDTAASGADTFFPAFDPRDWREVARVHHPADAKHPFAFDFVDYER
                     R"
     gene            1340..2374
                     /locus_tag="VFK00_09840"
     CDS             1340..2374
                     /locus_tag="VFK00_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593292.1"
                     /translation="MNHALVAGVLSFAAMAASPAFGGSPGSKVLPTVYEAGHFYATPE
                     TTNGQNLRLLVDTGGGGRYGMYWITTAAARRLLLKTRACKIGDSSVTVADVPEYQRGH
                     GLPPPSGPCGNVVMVQNVPGTYAGISHDDGQFSGSYLYAKGVWTFDYPGQRLILQGNA
                     WRPHPAAHPTPLGFQRDDQGHATFGYARITIRVDGQPLDLLLDTGATAHPTPAGEQAS
                     GAPVVNGSGVTSYITASVFDRWHRAHPDWRVVREGDELFGPRHPMPIIEVPEVEIAGW
                     SVGPVWFTERPDPAFHDMMSSLMDKRIEGAVGGNVFRHFVMTIDYPQATAYFRCASGC
                     KSAAKPRQVP"
BASE COUNT          437 a          817 c          767 g          393 t
ORIGIN      
        1 tgccgtggcg cagaccggcg acaacgtggc gaagtccgca ttcaactccg cagcgcgctc
       61 cagttcttcc gcgtcatggc acgatgcacc tacccacgaa ccgagtggta gcggtcgctc
      121 gaccaagtgg cgcaactgcg aagccttgag gtgcacgccg acgcccaact ctcgcgcgag
      181 ctcgacgtcc cggttgatca gcagcgaccc tcggacctcg gggttggtcg ccaatgcatc
      241 ggacgcgaac cgtctgatct cgtcgcgacc ggcttcgggc attcgcagtt gccacaacac
      301 tcgttcgggt acggcgccat gcgcaacgag ggccggcgca agcaatgctc ccgacgcaac
      361 aatggcgtag caaggcggca ggtgaagcgc ggtgacgatc ggcgcgtcgg cgggtggcat
      421 cgtgctcacg tcgatgtcgg gcaacgcgga ccagcgcagc ggatggcctt cccgcgcgcg
      481 cggctcaccg cgccacgccg tcacgcgcag ggcgtgcaga cgcacggcct tttcggggta
      541 tcgccacggc aacgagatca atggcgccga cgcgagtacc gcgatgccga gttcctcgtt
      601 caactcgcgg accagcgcct cgtcgatggt ttcaccaggt tcgaccttgc cgcccggaaa
      661 ttcccacagg ccggcgagat gcttgccagg cagccgctgc atcaacaaca cgcgaccgcg
      721 gtcgtcgacg aggatgccgg cggcgacttc gatcatcgcc gttcgtaatc gacgaaatcg
      781 aacgcgaagg ggtgtttggc atcagccggg tgatgcacgc gcgcaacctc gcgccaatcg
      841 cgcggatcga acgcggggaa aaacgtgtcg gcaccggaag cggcagtgtc gatttccgtc
      901 agatgcaggc gcgtggcgca cggcagcgcc aatgcataga cttcgccacc gccgatcacg
      961 cacaggtccg aatcacccgc gtgcgcgacg gcctcgtcca gcgaatgcac cacggactgt
     1021 cccgcgaacg gcgcggtatc ggcgcgtgtc agcacgaggt tcgggcgtcc cggcaacggt
     1081 cggccgattg cgagcgcggt cttgcggccc atcagcaccg gcttgccgag cgtcagcgcc
     1141 ttgaaccgct tcaggtcgtc cggcaggtgc cacggcattg cgccggcacg gccaatggcg
     1201 cgattgcggt cgagcgcggc gatcaatgaa atggacacgg gcgcgggcac catccggaag
     1261 tcatggtgtc gagcggccca tgcaataacg caacagccac cctaacctgc gtggaatcgt
     1321 aacgaatcgg aggcccgaca tgaaccatgc tctcgttgcc ggcgtcctgt ctttcgccgc
     1381 gatggccgcg tcgcctgcgt ttggcggatc ccccggcagc aaagtgctgc ctacggttta
     1441 cgaagcgggc catttctatg ccaccccgga aaccaccaat ggccagaatc tcaggctgct
     1501 ggtcgacacc ggcggcggcg ggcgctacgg gatgtactgg atcaccaccg ccgccgccag
     1561 gcgcctgctc ctcaagaccc gcgcctgcaa gatcggggac agctccgtca ctgtcgccga
     1621 cgttccggag taccaacgcg gccatggcct cccgccaccg tcgggcccat gtggcaacgt
     1681 cgtcatggtc cagaacgttc ccgggaccta cgccggtatc tcgcacgatg acggacagtt
     1741 cagcggcagc tatttgtacg ccaagggtgt ctggacgttc gactatcccg gtcagcgcct
     1801 catcctgcaa gggaatgcct ggcgcccgca ccccgcggcg caccccaccc cgctcggatt
     1861 ccagcgtgac gatcagggac acgcgacttt cggctacgcc cgtatcacga tccgcgtcga
     1921 tggccagcca cttgacctgc ttctggatac cggtgcgacc gcgcacccca cacccgcggg
     1981 cgaacaagcc agcggtgctc ccgtcgtcaa tggatccggc gtgaccagct acatcaccgc
     2041 cagcgtgttc gaccgctggc accgggcaca tccggactgg cgggtcgtaa gggagggtga
     2101 cgagctgttc ggacccagac accccatgcc catcatcgag gttccggagg tcgaaattgc
     2161 cggctggtcg gtggggccgg tgtggttcac cgaacgtccg gacccggcct tccacgacat
     2221 gatgtcgagc ctgatggaca agcggatcga aggcgccgtc ggtggcaacg ttttccgcca
     2281 cttcgtgatg accatcgact atccgcaggc aacggcgtat ttccgctgcg cgagtggctg
     2341 caagtctgcc gctaaaccgc gacaggtgcc ttgattgccg ggtgcggatc gtaatcgacg
     2401 atgcggatgt cttc
//
LOCUS       DASSNH010000267         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_39714, whole genome shotgun sequence.
ACCESSION   DASSNH010000267 DASSNH010000000
VERSION     DASSNH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39714"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFK00_09845"
     CDS             <1..498
                     /locus_tag="VFK00_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7593293.1"
                     /translation="DFPGVPVINRGFGGSAIADSTHYADRIVIPYHPRVIVMYAGDND
                     VEERCTPRQVLDEFEAFVSRVRRDLPDVAIVYISIKPSLARWSLWPRMREANREVERW
                     ARTQARMTFVDVAPRMLGPDGKPRADLFREDGLHMTPAGYAIWVEALNPVLAGYGFRP
                     HATGD"
     gene            645..1610
                     /locus_tag="VFK00_09850"
     CDS             645..1610
                     /locus_tag="VFK00_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593294.1"
                     /translation="MPSMLGKREWTMGAAGALAIAAGAYALRRETVSRRRGGRGHWVP
                     VSAHRAAIVEAAQRLNRGAGLIATSTLLDSAVEHFRGSFHNKAMFTPLVASALTLAVS
                     VHGTADKRKIAHKVRDPVYAAAAITGLVGTGFHIYNVTRRPGGFCWQNLFYGSPLGAP
                     AAVALSGLLGYYSERLRDSHSMRPRVFGFRAGRAIAALTSVGLLATAAEATLLHFRGA
                     YHDPFMFVPVTVPPVGALLLAETAASHPRSPRRFAGWWLRLTAVVGFIGSAFHALGVS
                     RNMGGWRNWRQNVLNGPPIPAPPSFTGLALAGLAALDLLRKHPNA"
     gene            1603..2298
                     /locus_tag="VFK00_09855"
     CDS             1603..2298
                     /locus_tag="VFK00_09855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HET7593295.1"
                     /translation="MHDRYPGYDVLRKRDTPSWNDATRSVIDARLAIPDAARFLAAEE
                     WQTLTAVCDRIVPQPAGRPRVSIAALVDHKLANDHRDGYRQASLPPLREAWQRGLAAL
                     DAEAQARHQRRFHALTVESQDELLKAMQDGKLDGPAWQGMSPGTFFSERVTHDILAAY
                     YSHPTSWNELGFGGPASPRGYVRMYFDRRDPWEAVEAKPGHEEEARRANQQVGTWKPE
                     SLTRMNADEEEQH"
     gene            2410..4074
                     /locus_tag="VFK00_09860"
     CDS             2410..4074
                     /locus_tag="VFK00_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7593296.1"
                     /translation="MSAKSNDLHDALHNPRGKDGRAPDPLRVGGWLPMRQYREDDEVD
                     FAIVGTGAGGGTLACRLAEAGFSVVAFDAGAWWRPLEEFASDETHQAKLYWTDERICD
                     GDNPLKLGSNNSGKSVGGSTVHFAMVSLRFRPEWFKSRSLLGYGADWPIDWREMWDYY
                     TEVEQALKIAGPVTYPWGPPRPRYPYRAHEVNAAGWVLAKGCEAMGIPWTETPLATIS
                     APRGLAHPCVYRGFCVTGCATNAKQSALVTWIPRAVRAGAEIRDLAMVGRIETHDAGL
                     ATGVHFHREGKWRFQRARNVVVAGYAIETPRLLLNSASARYPDGLANSSGLVGKNLMV
                     QTNQGAFGVMDEEIRWYKGPPSLTLTEHWNYTDAGKDFFGGYAFMAQGPLPQAWASTQ
                     AGNRGLWGEALLREMEKYNHQAGLKIVGEVLPQERNRVTLADEKDQYGLPVARVTFSL
                     CDNDKALVKHSIDFMTQTLCAAGAHDVWAESDDTAHLGGTARMGDDPRSSVVDGDCRS
                     WDIPNLWVCDGSVFPTVGGANPSLTIQAIACRTADRIRALAARGEL"
     gene            4257..>4726
                     /locus_tag="VFK00_09865"
     CDS             4257..>4726
                     /locus_tag="VFK00_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7593297.1"
                     /translation="MSSPMVSDFLLNRLHEWGVRRMYAYPGDGINGLMGALDRIGDKI
                     KLVRARHEEMCAFMACAHAKFTGEVGVCMATSGPGAVHLLNGLYDAKLDHQPVLAIVG
                     QQARTALGGHYQQEVDLPNLFKDVASEFVEMATVPAQIRHLVDRAMRIAMSKKTV"
BASE COUNT          807 a         1528 c         1599 g          792 t
ORIGIN      
        1 gatttccccg gcgtgcctgt catcaatcgc ggattcggtg gctcggcgat cgccgacagc
       61 acgcactacg cggaccggat cgtcattccc taccacccgc gcgtgatcgt gatgtatgcc
      121 ggcgacaacg acgtcgagga acgctgcacc ccgcggcagg tgctggacga attcgaggca
      181 ttcgtgtcgc gcgtgcgccg cgacttgccg gacgtggcga tcgtgtacat ctcgatcaag
      241 ccgagcctcg cgcgctggtc gctgtggccg cggatgcgcg aggcgaatcg tgaagtcgaa
      301 cgctgggcgc ggacgcaggc gcggatgaca ttcgtcgatg tcgcgccgcg gatgctgggt
      361 ccggacggga agccgcgcgc ggacctgttc cgcgaggatg gcctgcacat gacgcccgcc
      421 ggttatgcaa tctgggtcga ggccttgaac cccgttctcg ccggttacgg cttccgcccc
      481 cacgccaccg gcgattgaac gaagcgcgcg tgtggctccc gcgagcgtcc agcgaaggaa
      541 gcgtttcaca cgtgcttcat cccgtcgcga ccaacatgcc gcatgcggcg agcgccggtg
      601 cggagcgacc gcgcgtgcgc gccgttgcat gcaatggaat gacgatgcca tcgatgctcg
      661 gcaaaaggga atggacgatg ggcgcggccg gcgcgctggc gatcgctgcc ggtgcctacg
      721 cgctgcggcg ggaaacggtt tcgcgccggc gcggtgggcg cgggcactgg gtgccggtct
      781 ccgcccatcg agctgccatc gtcgaagcgg cgcagcggtt gaatcgtggc gccggcctga
      841 tcgcgacctc cacgctgctg gacagcgcgg tcgaacattt ccgcggatcg ttccacaaca
      901 aggcgatgtt cacgccgctg gtcgcttccg ccctgacgct cgccgtgagc gtgcacggca
      961 ccgccgacaa gcgcaagatc gcccacaagg tgcgcgatcc ggtgtacgcc gccgccgcga
     1021 tcaccggcct ggtcggtacc ggcttccata tctacaacgt caccaggagg cccggcggct
     1081 tctgctggca gaacctgttc tatggttcgc cgctgggcgc gcccgccgcc gtcgcgttgt
     1141 cgggattgct ggggtattac tcggagcgct tgcgcgacag ccattcgatg cggccgcggg
     1201 tattcggatt ccgcgccggc cgtgcgattg ccgcgctgac gagtgtcgga ctgctcgcca
     1261 ctgcggccga agcaacgctc ctgcatttcc gcggcgccta ccacgatcct ttcatgttcg
     1321 tgccggtgac ggtgccaccg gtcggagcgc ttttgctggc ggaaaccgcg gccagccatc
     1381 cgcgcagccc tcggcgcttc gccggttggt ggctgcggct gaccgcagtg gtcggattca
     1441 ttggatccgc gttccacgcc ttgggcgtgt cgcgcaacat gggtggctgg cgcaactggc
     1501 ggcagaacgt gttgaacggg ccgcccattc cggcgccacc gagctttacc ggcctcgcct
     1561 tggctggcct cgcggccctc gacctgttga ggaagcaccc gaatgcatga tcgatacccc
     1621 ggttacgacg tgctgcgcaa acgcgacacg ccgtcctgga acgacgcgac gcgcagcgtg
     1681 atcgatgcgc gcctcgcgat tcccgatgcc gcgcgattcc tcgcggccga agagtggcag
     1741 acccttacgg cagtctgcga caggatcgtg ccacaacccg cgggccggcc gcgggtttcg
     1801 atcgcggcac tggtcgacca caagctcgcg aacgaccacc gggacggcta tcgacaagcg
     1861 agcctgccgc ccttgcgcga ggcgtggcag cgcggactcg cggccctcga cgcggaggcg
     1921 caagccaggc accagcggcg tttccatgcg ttgacggtcg agtcccagga cgaattgctc
     1981 aaggcgatgc aggacggcaa gctcgacggt ccggcgtggc aggggatgtc tccaggtacc
     2041 ttcttttccg agcgggtcac ccacgacatc ctcgccgcct actactcgca cccgacgtca
     2101 tggaacgaac tcggcttcgg cggccccgca agtccgcgcg gctacgtgcg catgtacttc
     2161 gaccgccgcg acccctggga ggccgtcgag gcgaaacccg gccatgaaga agaagcgcgg
     2221 cgggccaacc agcaggtcgg aacctggaag ccagaatccc tgacgcggat gaacgcggat
     2281 gaggaggaac aacattgata cggattcaga aagagctttg acgcggatga acgcggatag
     2341 agctctttac cttccatcag ctttgtccgc gtaaatccgc gtcgacagcc cctttgccat
     2401 tccacgacca tgagtgccaa gtcgaacgat ctgcatgacg cgttgcacaa cccgcgcggc
     2461 aaggacggcc gcgcacccga tccactgcgg gtcggcggtt ggttgccgat gcggcagtac
     2521 cgcgaagacg atgaagtcga tttcgcaatc gtcggtaccg gcgcaggcgg cggcaccctg
     2581 gcgtgccgcc tggcggaagc cggtttctcc gtggtcgcct tcgacgccgg tgcgtggtgg
     2641 cggccgctcg aggaattcgc ttccgacgaa acccaccagg ccaagctcta ctggaccgac
     2701 gaacgcatct gcgacggcga caatccgctg aagctcggca gcaacaacag cggcaagtcg
     2761 gtcggcggct cgacggtgca cttcgccatg gtgtctctgc gcttccgtcc cgaatggttc
     2821 aagtcgcgca gcctgctcgg ctatggcgcc gactggccca tcgactggcg cgaaatgtgg
     2881 gattactaca cggaagtgga gcaggccttg aagatcgcgg ggccggtcac ctatccgtgg
     2941 gggccgccgc gcccgcgcta tccgtatcgg gcgcacgaag tgaacgcagc cggctgggtg
     3001 ctcgccaagg gttgcgaggc gatgggcatc ccgtggaccg aaacgccgct cgcgacgatc
     3061 tcggcgccgc gtggccttgc gcatccttgc gtgtaccgcg gcttttgtgt aaccgggtgc
     3121 gcgaccaacg ccaagcagag cgcgttggtc acctggattc cccgcgcggt cagggccggg
     3181 gccgaaatcc gcgatctcgc gatggtcgga cgcatcgaaa cccacgatgc gggtctcgcg
     3241 acgggcgtgc acttccatcg cgagggcaag tggcgtttcc agcgtgcacg caacgtggtg
     3301 gttgcgggct acgcgatcga gacgccgcgg ttgctgctga attccgccag cgcgcgctat
     3361 ccggacggac ttgccaacag ctcgggtctc gtcggaaaaa acctgatggt gcagaccaac
     3421 cagggcgcgt tcggcgtgat ggacgaggag atccgctggt acaagggacc gccgtcgctg
     3481 accctaaccg agcactggaa ctacaccgac gcgggcaagg acttcttcgg cggttatgcc
     3541 ttcatggcgc aggggccgct gccgcaggcg tgggcttcga cgcaggccgg caaccgcggt
     3601 ttgtggggtg aggcgctgtt gcgcgagatg gaaaagtaca accaccaggc ggggctcaag
     3661 atcgtcggcg aggtgctgcc gcaggaacgc aaccgcgtaa ccctcgccga tgaaaaggac
     3721 cagtacggat tgccggtcgc gcgggtgacc ttttcgttgt gcgacaacga caaggcactg
     3781 gtgaaacact ccatcgattt catgacgcaa acgctttgcg ccgcaggcgc gcacgacgtc
     3841 tgggcggaat ccgacgacac cgcgcatctc ggcggcacgg cccggatggg agacgatccg
     3901 cgcagcagcg tggtcgatgg cgattgccga agctgggaca tcccgaacct ctgggtgtgc
     3961 gacgggtcgg tgttccccac cgtcggtggc gccaatcctt cattgacgat ccaggcgatc
     4021 gcctgccgca ccgcggatcg catccgcgcc ctcgcggcgc gcggtgagct ctgacgggaa
     4081 gtggttctcc gcgggtcggg atgaacgggg gcgcgatcca tggcccatga ttcacggccg
     4141 tttcacctgt tgtcgcgcac agtgattctt cgacatggcg tcgatgaccg cccgggcggg
     4201 cgtggaacgc attacgcagt ttctcggcgc agcaatacga caaggaggtt ccattcatgt
     4261 catcgcccat ggtcagcgat ttcctgctca accggctgca cgaatggggc gtgcggcgta
     4321 tgtacgccta cccgggcgac gggatcaacg gcctgatggg ggcgctcgac cgcatcggcg
     4381 acaagatcaa gctggtgcgc gcgcgccacg aggagatgtg cgcgttcatg gcctgcgcgc
     4441 atgcgaagtt caccggcgaa gtcggcgtgt gcatggcgac ttcgggcccg ggtgcagtgc
     4501 acctcctgaa tgggttgtac gacgccaagc tcgaccacca gccggtgctc gcgatcgtcg
     4561 gacagcaggc gcgcacggcc ctcggcggac actaccagca ggaggtcgat ctgccgaacc
     4621 tgttcaagga tgtcgcgagc gaattcgtgg aaatggcgac ggtgcccgcg cagatccggc
     4681 acctggtcga ccgggcgatg cggatcgcga tgtcgaagaa gaccgt
//
LOCUS       DASSNH010000268         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_709308, whole genome shotgun sequence.
ACCESSION   DASSNH010000268 DASSNH010000000
VERSION     DASSNH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709308"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2868
                     /locus_tag="VFK00_09870"
     CDS             <1..>2868
                     /locus_tag="VFK00_09870"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HET7593298.1"
                     /translation="ALKTYLAAVEQAFRAGNATEHTYRPAFKALIEALAGPGITAVNE
                     PRQIDCGAPDFIIVRGTVPLGYIEAKDVGTDLARIEKGEQLARYRESLGNLVLTDYLE
                     FRWFLDGELRLTAALPRPDRNGRIRWNGDAASEVAHLLDQFLSADIPLKSTPQDLATR
                     MAKLALLIRGLITRAFKSEQGEGELHAQFEAFRKVLIESLTIEQFADMYAQTLAYGLF
                     AARCNAPAAGFTRQSAAAQLPRTNPFLRRLFNSIAGPDLDPRVAWAVDQLAELLARAD
                     MSAILEDFGRRTRREDPVVHFYETFLAAYDPKMREARGVYYTPEPVVNYIVRSVDHLL
                     QREFGLKDGLAHSGKLRWKTVMAEATHSRRKSSTRIEEKAPWVHRVQILDPATGTGTF
                     LYAVIQKIRERFEGNAGAWPGYVAEHLLPRLFGFELLMAPYAVAHMKLGIELEQSGYD
                     FSSDERLGVFLTNSLEEAHEKTGLPLFTQWLADESQAASRVKRDVPVMVVLGNPPYSG
                     HSANKGEWISQLLHGEDTLNHCRTESYFECDGKPLGERNPKWLNDDYVKFIRFAQWRI
                     EQTGHGVLAFVTNHGYLDNPTFRGMRESLLRGFDTIWLYDLHGNTKKKEQAPDGGKDE
                     NVFDIQQGVAIGIFVKHASLPSPSGRGAGGDGTAELERRGDPEPPSGASRHLPPEGEG
                     KSLARVFHADLYGTRALKYGTLDAESVADTPWTELAPASPSYYFVPEDGALREEYERG
                     WKITEVMPVNSVGIATARDALTIHFTDDETWQTVSRFAELSEDEARETFQLGRDARDW
                     KVALAQEDVRRSGPSRDRVTPIHYRPFDVRSTYYTGNSRGFQCMPRPAVMQHFFQHDN
                     LGLVTSRLTKGENFAHAQMTRHITEVICMSPKTSNNGFVFPLWLYPRADEDLLDAAPK
                     QKTANLAPAFVTALTEAAGDTPTPEDTLAYIYAILYAPSYRA"
BASE COUNT          551 a          954 c          896 g          467 t
ORIGIN      
        1 agcgctgaaa acctaccttg ccgccgtcga gcaggcgttc cgtgcaggca acgccaccga
       61 gcacacctac cgccccgcct tcaaggcact gatcgaggcg ctggccggcc ccgggatcac
      121 ggcggtcaac gagccgcgcc agatcgattg cggcgctccc gacttcatca tcgtgcgcgg
      181 tacggtgccg ctgggctaca tcgaagccaa ggacgtcggc accgatctcg ccaggatcga
      241 gaagggcgag cagctcgccc gttaccgcga atcgctcggc aacctggtgc tcaccgatta
      301 cctggagttc cgctggttcc tcgacggcga attgcgcctg accgccgcgc tgccgcggcc
      361 ggacaggaac ggccgaatcc gctggaacgg cgacgcggcg tccgaagtcg cgcacctgct
      421 ggaccagttc ctctccgccg acattccgct taagtccacc ccgcaggacc tcgccacccg
      481 catggccaag ctcgcgctgc tgatccgcgg actgatcacg cgcgccttca agtcggagca
      541 gggcgaaggc gaactgcacg cgcagttcga agccttccgc aaggtcctga tcgaatcgtt
      601 gaccatcgag cagttcgccg acatgtatgc gcagacgctg gcctacggcc tgttcgcggc
      661 gcgctgcaat gcgccggcgg ccggcttcac gcgccagagc gcggccgcgc aactgcccag
      721 gaccaatccg ttcctgcgca ggctgttcaa ttcgatcgcc ggaccggacc tcgacccgcg
      781 tgtcgcctgg gcggtcgacc agcttgccga gctgctggcg cgcgccgaca tgagtgcgat
      841 cctcgaggat ttcggccgcc gcacccgccg cgaagacccc gtggtgcact tctacgaaac
      901 cttcctcgcc gcctacgacc cgaagatgcg cgaggcccgc ggcgtctatt acacgcccga
      961 gccggtggtg aactacatcg tgcgcagcgt cgaccacctg ctgcagcgcg aattcggtct
     1021 gaaggatgga ttggcgcatt cgggcaagct gcgctggaaa accgtcatgg ccgaggccac
     1081 ccactccagg cgcaagtcgt ccacgcgcat cgaagagaag gcgccgtggg tgcaccgcgt
     1141 gcagatcctc gatcccgcca ccgggaccgg tacgttcctg tacgcggtga tccagaagat
     1201 ccgggagcgc ttcgaaggca acgccggcgc atggccgggc tacgtcgccg aacacctgct
     1261 gccgcgcctg ttcggcttcg aactgctgat ggcgccgtat gccgtggcgc acatgaaact
     1321 cggcatcgag ctggaacaat ccggctacga cttttcgagc gacgaacggc tcggcgtgtt
     1381 cctgaccaat tcattggagg aagcgcacga gaaaaccggg ctgccgctgt tcacccagtg
     1441 gctggccgac gaatcgcagg cggcctcgcg cgtcaaacgc gatgtgccgg taatggtggt
     1501 gctgggtaat ccaccgtact cggggcattc ggccaacaag ggcgagtgga tttcgcaact
     1561 gctgcacggc gaagacacgc tgaaccactg ccggacagaa agctatttcg agtgcgacgg
     1621 caagcccctg ggcgagcgca atccgaaatg gctgaacgac gactacgtca agttcatccg
     1681 cttcgcgcag tggcggatcg agcagaccgg ccacggcgtg ctggcgttcg tcaccaacca
     1741 cggttacctg gacaatccga cctttcgcgg gatgcgcgaa tcgctgttgc gcggcttcga
     1801 cacgatctgg ctgtacgacc tgcacggcaa taccaagaag aaggagcagg ccccggacgg
     1861 cggcaaggac gaaaacgtgt tcgacatcca gcagggcgtg gcgatcggga tcttcgtgaa
     1921 gcacgcttcg ctcccctctc cctccgggag aggggctggg ggtgatggta cggcggagct
     1981 ggaacgccgc ggtgaccccg aacccccatc cggcgcttcg cgccaccttc ccccggaggg
     2041 ggaagggaaa agcctcgccc gtgtgttcca cgccgacttg tacggaaccc gcgcactgaa
     2101 atacggcacc ctggacgcgg aatcggtggc ggacacgcca tggacggagc tcgccccggc
     2161 cagccccagc tactacttcg tgccggagga cggcgcattg cgcgaggagt acgagcgcgg
     2221 ctggaagatc actgaggtga tgccggtgaa cagcgtcggc atcgcaaccg cgcgcgatgc
     2281 gctgactatt catttcactg atgacgaaac ctggcaaacg gtgagccggt tcgctgaact
     2341 atccgaagac gaggcacgtg aaaccttcca gcttggccgt gatgcgcgcg attggaaggt
     2401 ggcgttggca caggaggatg tacggagatc ggggccatcg cgagatcgtg tgactcctat
     2461 tcactaccgc ccctttgacg tgcggagcac ctactacacc gggaattccc ggggcttcca
     2521 atgtatgccg cgcccggctg tgatgcagca cttcttccag cacgacaact tgggccttgt
     2581 gacctcgcgg ctaacgaaag gcgaaaattt tgcgcacgcg cagatgacgc gtcacatcac
     2641 ggaagtcatc tgcatgtcgc caaaaacctc gaacaacgga ttcgtgtttc cgctttggct
     2701 ctatccccgc gccgacgaag atctgctgga tgcggcgccg aagcaaaaga ccgccaacct
     2761 cgcgccggcc ttcgtgacgg cgctcaccga agcggcgggc gacacgccga cgcccgagga
     2821 cacgctcgcc tacatctacg ccatcctgta cgcgccgtcg tatcgcgc
//
LOCUS       DASSNH010000269         9720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1656344, whole genome shotgun sequence.
ACCESSION   DASSNH010000269 DASSNH010000000
VERSION     DASSNH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656344"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..1819
                     /locus_tag="VFK00_09875"
     CDS             452..1819
                     /locus_tag="VFK00_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593299.1"
                     /translation="MLKKKGFALGVLAVLALAASFVPALAFASTSPAAAANTAWPRFF
                     VAQDGVALSQFPSRSRLAAVVRVLATQPVATPGRWRASSSAAWLSVTPHGRTGQPLRL
                     TADPTGLATDTLHSATITLRGTAGSSVIGSISMRVSLWIGSSDPGNQTIDHDAVSLAA
                     NPVEPWVYVSDGSSNIDVFNVYSGRLVRSFTGVATSVGRLQTSSDGSELFAVDTTNYA
                     IVALNAISGRILKLYALAGPISSDFNFAYARPAGISTLFAPGQAAIDVATGNEVSSPL
                     TGGFFYDPFITSTADGSHLAIVERGLSPGSLYSYTVASDHGYLTITQVNGSALAGSNC
                     PDVAISPDGSRLYPACGWPYEFDVYDFASLEQIQTLAATNYPNNAEFDSDGDFVGGLD
                     GIYNPADVFVFDVSGHSLGTVPEIEYAQSQGDALMQISGDSTRVISGMWNPGGQLMFR
                     EMPRP"
     gene            1955..2851
                     /locus_tag="VFK00_09880"
     CDS             1955..2851
                     /locus_tag="VFK00_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7593300.1"
                     /translation="MPFSRTIIFAALALVAFAGNSLLCRAALAHTHIDAASFTTVRLL
                     SGAVVLCCLALLRGGARTGRGNWRSALALFAYAAGFSFSYLHLTAAAGALLLFGAVQV
                     TMIGFGVWSGERMRSLQVVGLVLACAGLVGLLLPGLSAPPLPGALLMLGAGFAWGAYS
                     LRGKRGGDPLKITAGNFMRAVPMALGLSLVLFARASPDPAGIGYAFLSGALTSGVGYA
                     IWYAALPALKSTTAATVQLSVPVLAAAGGVFFLGEPLTLRLLIASMAILGGIALVILT
                     GDRRVKPAAPALVSGGSRPKPP"
     gene            2937..3329
                     /locus_tag="VFK00_09885"
     CDS             2937..3329
                     /locus_tag="VFK00_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7593301.1"
                     /translation="MTQVPSGNELIRAFLPTRDFDLSKRFYEALGFEKVLDGDVAIFN
                     AGSGGFILQRYHQKDWAENAMVQLMVDDLGAWWARIQSLDLPERFGVPAPRAPAVQPW
                     GLRIAYVVDPAGVLWHVAERRASEKQDQ"
     gene            3342..3734
                     /locus_tag="VFK00_09890"
     CDS             3342..3734
                     /locus_tag="VFK00_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7593302.1"
                     /translation="MPMLVESCIPVVPCADLEKSLRFWVEGLGLTMDRDMQQDGHLVG
                     CMVHNEHLSFWLNRRAGLPDRPRDYEGLRLYWAPRDLHAMRERLLHLGYPVSEIVERD
                     YGQTEFFVTDDDGYSHCFGVESRKWEQS"
     gene            complement(3951..5492)
                     /locus_tag="VFK00_09895"
     CDS             complement(3951..5492)
                     /locus_tag="VFK00_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7593303.1"
                     /translation="MSLVDLIFIGLGAVFGSGWLFSASYVADIAGPAGIVSWLIGGAA
                     VLLLGLVYCELGAALPHSGGVVRYPVYSHGSLLGFLMGLITLIAFSSLIAIEVTACRQ
                     YASAWFPQLTQAESQSPTPLGWLVQLALLLVFFLLNYYSVKTFARANNLVSVFKFVVP
                     LTVIVVLLWFFHGGNFTAHGFAPFGFAGIAGAISAGGIIFAYLGLTPIVAVAGEVRNP
                     QRNIPIALVLSVVLSTAIYLLLQVAFLGSVPAAMAEHGWKGIDKLFALPFHDIAIALG
                     IAWLSFLVVCDAVISPSGCGNIYMNATPRVVYGWARGGTFFKMFTRVDAKSGIPRPAL
                     WLTLALSIFWTLPFPSWSAMINVVSAALVLSYAVAPVTTAALRRNAPDMPRPFRVRGF
                     VVLGPLSFIVAALIVYWSGWGTVSWLLGLQVVLYALYAAYVARHRSRRAQLAQDVRAS
                     LWLVGFYALVLAASWLGTFGGRHVVEGPWDSLLVAAIAVLVYYWGERSGVPFSRSAWV
                     AEDRT"
     gene            complement(5623..6978)
                     /locus_tag="VFK00_09900"
     CDS             complement(5623..6978)
                     /locus_tag="VFK00_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7593304.1"
                     /translation="MTVRKLVFGITLLAITVVAWAAPPPHLQAASAATGTFLLTADRV
                     FDARSEQAHAGWAVLVRGDKIAAVGPRDSIEVPSAATEIDLPGTTLLPGLIDAHSHIF
                     LHPYNETLWNDQVLKEPLPYRTIEAVQHVRDTLMAGFTALRDLGTEGAGYADVQVQKA
                     INDGLIPGPHLFVATRATIARDCYGPGPMGFRTDMTIPQGGIPVAGVPEMIAAVRDQA
                     GHGADWIKIYADYHCGKSHGSVPTFTQQELNAGVEAAHSLGLPVSVHSSTAEGMRRSI
                     LAGVDTIEHGFGGTREVFEMMAKHDIAYLPTLEAVVAYSQYFQGWKPGDPPTDDMKQS
                     QHAFKLAIQAGVTIGCGSDVGVFTHGTNYKELEWMVKDGMRPAQALLAATAVDSKILR
                     QQDQFGQLKPGLDADIIAVQGDPTKDIAATEHVPFVMKGGVIYKQPSAAQPMAPAPAS
                     K"
     gene            7118..7615
                     /locus_tag="VFK00_09905"
     CDS             7118..7615
                     /locus_tag="VFK00_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7593305.1"
                     /translation="MKVILTFAAGILFAAAATASHPESSRHSIEHGKYLVGYGGCNDC
                     HTPGWAEHGGEAPRDMLLTGGGMNFQGPWGTTYAPNLRLYMANLTLDQWIHNARNMKA
                     RPAMPFWTFRYLSDKDLGDMYAYIRSLGPGGKPAHDYVPPGQPAPLPYFKLALPPPPV
                     RPAGN"
     gene            7626..8141
                     /locus_tag="VFK00_09910"
     CDS             7626..8141
                     /locus_tag="VFK00_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593306.1"
                     /translation="MNGSWFLVLRAGHVLLAALWLGAAFMLTMYVLPAVRDAGPTGSH
                     FMQTLQRRKLHVFMALAAILTVLTGIWLYWRFTSGLDTDVMLSPGGLAFGIGGICGLL
                     AMILGGAIMGRGFARIAALGERAAAGPEAERTTHLQDIEVLRQRLVVAGKLILLLMVL
                     ALVLMAIGHYL"
     gene            complement(8331..>9720)
                     /locus_tag="VFK00_09915"
     CDS             complement(8331..>9720)
                     /locus_tag="VFK00_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7593307.1"
                     /translation="DYGRIRPWCRRCDDGARIVAGPDALQLHSPPPLEQEDGAWCARF
                     TVRRGERVAFMLTWHNAWRDGPAQRDAGRSLRQTERFWRKWGRRYMQSNPWRDEVLRS
                     LLTLKSLSDDRTGAVIAAPTLGLPEIPGGVKNYDYRYTWLRDATFTLYAMIHSGYHDE
                     ARMWRNWLIDTTAGDPARLQPVYRVDGACRITQQKAHHLRGYERSQPVLIGNKAWRQT
                     QMDGYGEVINGVHVAHEHGLAMHQEDFDEQIGMVEYLEDHWQKAGTGLWERGKHVGAY
                     THSQVMVWVAVDRVARLIENSTFEGDAARWRALADSIHAHVCDYGFDRKRNTFVQRYG
                     SRAIDAAALRMPIVGFLPADDPRMLGTIAAIEKHLCRDGFVWRYGEEDGEVPEEGSFL
                     VCSFWMVEILVMLGRKQEATEMFQRLLDVRNDVGLLSEEYDPGSGRLRGNFPQVFSHV
                     GLVNAAHRLAAD"
BASE COUNT         1634 a         3234 c         3125 g         1727 t
ORIGIN      
        1 gatcccgaga gcgatcatcg caacggcgaa tagcatgcgg ctcggttgca gcgtcgtgtt
       61 cacggcgtca cctccggttt ggggggttac ccatccgtta tgtacaacgt gtggcggcac
      121 tttaccgctt ccggcatcga gcttcctgtg cacgtgtatt tcgcgatgcc cccccccgca
      181 cggcgccggc atcaaatcgc ttgggcgact cagagatgca gtgtcgggtt attgagactc
      241 atttgcgtat tcagcaggcg catgcgcact tgttgcgctg aaaggccggt gggaagatgg
      301 cggcggggaa gcaccctcag aaagtagctt gcatcaagcg gaagccgggt ccgggggtct
      361 tcgtgtccgg aattcgtcca acgccatgtc tgtcggcccc gcgcaaagcc gacgcgcttt
      421 cctgcctgcg ccttcaacga ggaacgccat cgtgctcaag aagaagggtt tcgcgcttgg
      481 tgtgctggca gtgctggcgc tcgcggccag ttttgttcct gccctggctt ttgcctccac
      541 gtcccccgcc gccgctgcaa acaccgcttg gcctcgattc tttgtcgcgc aggatggcgt
      601 ggcattgtcg cagttcccct cgcggagccg gctggcggca gtagtgcgcg tcctcgcgac
      661 ccagccggtc gccacacctg gccgttggcg cgcgagctcc agcgcggcgt ggctcagcgt
      721 gactccccat ggcaggacag gacaaccgct ccggctgacc gcggatccaa cgggactggc
      781 caccgacacc cttcactccg caaccatcac ccttcgcgga actgccgggt cgtcggtcat
      841 agggagcata tcgatgcgtg tctctctctg gatcggctca agcgatccgg gcaaccaaac
      901 cattgatcac gacgctgtat cactggccgc caatccggtc gaaccgtggg tctatgtgag
      961 cgacggctct tcgaacatcg acgtgttcaa tgtctacagc gggcggttgg ttagatcgtt
     1021 caccggcgtg gcaacgagcg tcggacgatt gcagaccagc agtgacgggt cggagctgtt
     1081 cgcggtcgat acgacgaact acgcgatcgt tgccttgaac gcgatctccg ggcggatcct
     1141 taaactgtac gcgttggccg gtccgataag cagcgacttc aatttcgcgt acgcgcgccc
     1201 ggcaggcatt tccaccctct ttgcgcctgg ccaggcagcc atcgacgtcg ccacgggaaa
     1261 tgaggtgtcc tctccgctta ccggaggatt cttctacgac ccgtttatca cctccacggc
     1321 cgacggatcg cacctcgcca tcgtcgagcg tggcttgagc ccgggaagct tgtattcgta
     1381 caccgtggca agcgaccacg ggtacttgac gatcacgcag gtgaatggct cggcccttgc
     1441 cggctcgaac tgccccgacg tggccatttc cccggacggt tcacgcctgt atccagcctg
     1501 cggatggccg tacgaattcg acgtctatga cttcgcatcg ctggagcaga tacagacact
     1561 tgcggcgacc aattacccga acaatgccga attcgactct gacggcgact ttgtcggagg
     1621 gctcgatggc atatacaacc ccgcagacgt ttttgtcttc gacgtcagcg gccacagcct
     1681 tggaactgtt ccagagatcg aatatgcgca gtcccaggga gacgcgctca tgcagatatc
     1741 gggcgattcg actcgcgtca tatccggcat gtggaatcca ggtggccagc tcatgttccg
     1801 cgaaatgcca cgtccgtgat gattgtaatc tgacaagtgt cgggggcgga gccgcggctc
     1861 cgccccttgt tttccggcag cgtgcaagca atcggggccg ctcaggttgg agtgaacgtc
     1921 gcgaacggct tcgcatggaa acggacaaga gcccatgcca ttctcgcgca cgatcatctt
     1981 tgcggcactg gcgctggtcg cattcgcggg gaactcgctg ctgtgccgcg cggcgctggc
     2041 gcacacccat atcgacgcgg cgagtttcac gacggtacgg ttgctgtcgg gcgccgtggt
     2101 gctgtgttgc ctggcgttgc tgcgcggtgg tgcccggacg ggacgcggca actggcggtc
     2161 ggcgctggcg ctgttcgcgt atgcggcggg cttttccttt tcctatctgc acctgaccgc
     2221 ggcggcgggc gcactgctgc tgttcggcgc ggtgcaggtc accatgatcg gcttcggcgt
     2281 ctggtccggc gaacgcatgc gctcgctgca agtcgtcgga ctggtgctcg cctgcgccgg
     2341 gcttgttggt ctgttgctgc cgggcctgtc ggcaccgccg ttgccgggtg cgctgttgat
     2401 gctgggcgcg ggtttcgctt ggggcgccta ctcgctgcgc ggcaagcgcg gcggcgatcc
     2461 gctgaaaatt accgcgggca acttcatgcg tgccgtaccg atggcgttgg ggctgagtct
     2521 cgtgctgttc gcacgcgcct cgccggaccc ggcgggtatc ggctacgcgt tcctgtccgg
     2581 cgccttgacg tccggcgtgg gctacgccat ctggtacgcg gcgttgccgg cgctgaaatc
     2641 caccactgcc gcgaccgtgc agcttagcgt acccgtcctg gcggcggccg gtggcgtgtt
     2701 ctttctgggc gaaccgctga cgttgcgctt gctgatcgcc tcgatggcga ttctcggcgg
     2761 catcgcactg gtgatcctga ccggcgaccg acgcgtcaag cccgccgcgc ccgcgctggt
     2821 ttctggcgga agccgtccga aaccgccttg acccgggaaa agggcggccg ttgcagcaat
     2881 gtcgcgattc cggtctactt ccgccgcgcg caccgaaccg cctcggggta catggcatga
     2941 cgcaggttcc atcaggcaac gaactgatcc gggcattcct gccgacccgg gatttcgatc
     3001 tctcgaagag gttctacgaa gcgctggggt tcgagaaggt gctggatggc gacgtggcca
     3061 ttttcaacgc cggctcgggc ggcttcatcc tgcagcgata tcaccaaaag gactgggcgg
     3121 aaaatgccat ggtgcagttg atggtcgatg atctcggcgc gtggtgggca cgcatccagt
     3181 cattggactt gcccgaacgt ttcggcgttc ccgcaccgcg cgcgcccgcc gtgcagccat
     3241 gggggctccg cattgcctac gtcgtggatc ccgccggtgt gctctggcac gtggcggagc
     3301 ggcgagcgag cgagaagcag gatcaataat cgatcggagc gatgcccatg ctggtcgaat
     3361 cctgtatccc cgtggtcccc tgcgcggatc tcgaaaagag cctgcggttc tgggtggagg
     3421 gccttggact gacaatggat cgcgacatgc agcaagacgg ccacctggtc ggctgcatgg
     3481 tgcacaacga acacctgtcc ttctggctca accggcgcgc aggcttgccg gacaggccgc
     3541 gcgattacga ggggctgcga ctgtattggg caccccgcga tctccatgca atgcgcgagc
     3601 ggttgctgca cctcggctat ccggtgtcgg aaatcgtgga acgggattac ggacaaacag
     3661 aattttttgt caccgacgac gatggttaca gccactgctt tggcgtggaa tcccgcaagt
     3721 gggagcagtc gtgaggatca atcgcgcaac acttgccgat cgcattcacc ggactggcgg
     3781 tcaaggccct gaaccagggc acgtggtcga tcgcatcttt gccgcgcacc tgccacggca
     3841 ggcccatgcc tacgcttccg gcaacttcgg cgagatgccg acgctggaga acctgtccgc
     3901 acgatcctta cgcaagcacg tagttccccg ttaacgatgc ctgtaacccg tcaagtccgg
     3961 tcctcggcaa cccatgcgga tcgcgagaac ggcactccgc tgcgctcacc ccagtaatag
     4021 acgagcacgg caatggcggc gacaagcagc gagtcccacg ggccttcgac gacatgcctg
     4081 ccgccgaacg ttcccagcca ggaagctgcc agcaccaagg cgtagaagcc taccagccac
     4141 agcgaggcgc gaacgtcctg cgccagttgc gcgcggcggg aacggtgccg cgccacgtat
     4201 gccgcataca acgcatacag gacgacctgg agtcccagca gccacgacac cgtgccccac
     4261 ccggaccagt acacgatcag tgcggcgacg atgaacgaga gcggtcccag cacgacgaat
     4321 ccccggaccc ggaacgggcg cggcatgtcg ggcgcgttgc ggcgcagcgc ggcggtcgtg
     4381 acgggtgcca cggcatagct cagcaccagt gcggccgaga ccacgttgat catcgccgac
     4441 cacgacggga acggcagcgt ccagaagatc gacagcgcga gcgtcagcca cagcgccggg
     4501 cgcgggattc ccgatttcgc atcgacccgc gtgaacatct tgaagaaggt gccgccgcgc
     4561 gcccatccgt agaccacccg cggtgtcgca ttcatgtaga tgttgccgca gccgctgggc
     4621 gagatgacgg cgtcgcacac caccaggaac gacagccagg cgatgccgag cgcgatcgcg
     4681 atgtcgtgga atggcagcgc gaacagcttg tcgatcccct tccagccatg ttccgccatc
     4741 gcggccggaa cactcccgag gaaggcaacc tgcagcagca ggtagatcgc cgtcgacaac
     4801 accaccgaca ggaccagcgc gatcgggatg ttgcgctgcg gattgcgcac ctcgcctgcc
     4861 accgcgacga tcggcgtcaa cccgagatac gcgaagatga tgccaccggc ggatatggcg
     4921 ccggcaatgc ccgcgaatcc gaacggcgcg aagccgtgcg cggtgaagtt gcctccgtgg
     4981 aagaaccaca acagcaccac gatcaccgtc agcggcacca cgaacttgaa cacgctgacg
     5041 aggttgttgg cgcgcgcgaa ggtcttgacg ctgtagtaat tcagcaggaa gaaaaccagg
     5101 agcagcgcga gctgcaccag ccagccgagc ggcgtgggcg actgcgattc cgcctgcgtc
     5161 aactgcggga accaggcgga cgcatattgg cggcaggcgg tcacctcgat cgcaatcagg
     5221 ctggaaaacg cgatcagcgt gatcaggccc atcaggaaac cgagcaggga gccatgtgaa
     5281 tacaccgggt agcgcaccac gccacccgag tgcggcaacg cggcaccgag ttcgcagtac
     5341 accaagccca gcagcagcac cgcggcgccg ccgatcagcc aggacacgat ccccgcgggc
     5401 ccggcgatat cggcgacgta gctggccgag aacagccagc cggaaccgaa caccgcgccg
     5461 agcccgatga agatgaggtc gacgaggctc agctgcttct tgaagcggcc ctgccccttc
     5521 gcgggagcgt cctgcgcgac agtgctcgac gcggaggccg gatcgccagc cttcacggat
     5581 gccgcgccat gaaggttgcg gccgcgatgc cggccctgcc ggtcacttcg atgccggcgc
     5641 tggcgccatc ggctgcgccg ccgatggctg cttgtagatc accccgccct tcatcacgaa
     5701 cggcacgtgt tcggtcgcgg cgatgtcttt cgtgggatcg ccctgtaccg cgatgatgtc
     5761 ggcatccagc cccggtttca gttggccgaa ctggtcctgc tggcgcagga tcttcgaatc
     5821 gaccgcggtg gcggcgagca aggcctgcgc aggacgcatg ccgtccttca ccatccattc
     5881 gagttccttg tagttggtcc cgtgcgtgaa cacgccgacg tcgctgccgc agccgatggt
     5941 gacgccggcc tggatcgcca gcttgaacgc atgctgcgac tgcttcatgt cgtccgtcgg
     6001 tggatcgccc ggcttccagc cctggaaata ctgcgaatac gcaaccaccg cctccagcgt
     6061 cggcaggtag gcgatgtcgt gcttcgccat catttcgaac acctcgcgcg tgccgccgaa
     6121 gccgtgctcg atggtgtcca cgcccgccag gatcgaacgc cgcatgcctt cggccgtcga
     6181 cgaatgcacc gacaccggca ggcccagcga gtgagcggct tccacccccg cgttcaattc
     6241 ttgttgggtg aaggtcggca ccgagccgtg cgacttgccg cagtggtagt ccgcgtagat
     6301 cttgatccag tccgcgccgt ggccggcctg gtcgcgcacc gccgcgatca tctccggcac
     6361 ccccgcgacc ggaataccgc cctgcgggat cgtcatgtcg gtgcggaagc ccatcgggcc
     6421 cgggccgtag caatccctgg caatggtggc gcgcgtcgcg acgaacaggt gcggaccggg
     6481 aatcaggccg tcattgatcg ccttctgcac ctgcacgtcc gcgtaacccg ccccttcggt
     6541 gccgaggtcg cgcagcgcgg tgaatccggc catcagggtg tcgcgcacgt gctgcactgc
     6601 ctcgatggtc cgatacggca gcggctcctt cagcacctgg tcgttccaca gcgtttcgtt
     6661 gtacgggtgc aggaagatgt gcgagtgcgc gtcgatcagg cccggcagca gcgtggtccc
     6721 cggcaggtcg atctcggtcg ccgcggaagg cacctcgatc gaatcacgtg gtccgaccgc
     6781 cgcgatcttg tcgccccgga ccagcaccgc ccagcccgcg tgggcctgct cgctgcgcgc
     6841 gtcgaacacg cggtcggccg ttagcaggaa cgtgccggtc gcggccgacg ccgcctgcaa
     6901 gtgcggcggc ggcgcggccc aggccaccac cgtgatggcc agcaacgtga tcccgaaaac
     6961 cagtttgcgc accgtcatcc ccgcaccccc caccccgccg aagtgcgcag cctacacccc
     7021 tgggccgggg cttgcgatgc ggccacgagg gttcgatact tcgcgcgttg catcgagtca
     7081 ccggccgttt catcgcaacc gcaagagggg acacacgatg aaggtgatac tgacgtttgc
     7141 tgcgggcatc ctgtttgcgg cggccgcgac ggccagccat ccggaaagtt cacgacactc
     7201 catcgagcac ggcaagtacc tggtcggcta cggcggctgc aacgattgcc acacgcccgg
     7261 gtgggcggag cacggcggcg aggcaccgcg ggacatgctg ctgaccggcg gtggcatgaa
     7321 cttccagggc ccctggggca cgacctacgc gcccaacctg cgcctgtaca tggccaacct
     7381 gaccctcgac cagtggatcc acaacgcgcg caacatgaag gcgcgtccgg cgatgccgtt
     7441 ctggacattc cgctatctca gcgacaagga cctgggcgac atgtacgcct acatccgctc
     7501 gctcgggccc ggcgggaagc ccgcgcacga ctacgtgccg ccggggcagc ccgcgccgct
     7561 gccgtatttc aagcttgccc tgccgccacc cccggtgcgg ccggccggca actgaggacc
     7621 acgtcatgaa cggttcctgg ttcctggtgc tgcgggcggg ccacgtgctg ctggccgcgc
     7681 tgtggctggg cgcggccttc atgctgacga tgtacgtgct gcctgccgta cgcgacgccg
     7741 gaccgaccgg cagtcatttc atgcagaccc tgcagcggcg caaactgcac gtgttcatgg
     7801 cgctggcggc gatcctcacc gtgctcacgg gaatctggct gtattggcgc ttcacgtccg
     7861 ggctcgacac cgacgtcatg ctgagccccg gcggcctcgc cttcggcatc ggcggcattt
     7921 gcggattgct ggcgatgatc ctgggcggcg cgatcatggg ccgcggcttc gcacggattg
     7981 ccgcactcgg ggaacgggcc gccgcggggc cggaagccga gcgcaccacg cacctgcagg
     8041 acatcgaggt cctgcggcag cgactcgtcg tcgccggcaa attgatcctg ttgctgatgg
     8101 tcctggcgct ggtgctgatg gcgatcggcc attacctgta ggcaagcgtt gatcttgccc
     8161 gtcataccgg cgaaggccgg tatccggctc tcgttggatt ttgtgcgctt cgcgcgccat
     8221 gtttggctgg gccccggcct gcgccgggcg acgagctgcg tcgtcggttc cccggcgggc
     8281 ctgctccatt cgggagtgtg gcagggatcc tgccgcaatt tcctgccgcg ctagtccgcg
     8341 gcgaggcggt gcgcggcgtt gaccaggccg acgtgcgaaa acacctgcgg gaaattgccg
     8401 cgcaaccgtc cgctgccggg atcgtattcc tccgacagca ggccgacgtc gttgcgcacg
     8461 tcgagcaggc gctggaacat ttccgtcgcc tcctgcttgc ggccgagcat caccaggatc
     8521 tccaccatcc agaacgagca caccaggaag gaaccctctt ccggcacctc gccgtcctct
     8581 tcgccatagc gccagacgaa gccgtcgcgg cacaggtgct tttcgatcgc ggcaatggtg
     8641 cccagcatcc gcgggtcgtc cgccggcagg aacccgacga tcggcatgcg gagcgccgcc
     8701 gcatcgatgg cccggcttcc gtagcgctgc acgaaggtgt ttcgcttgcg atcgaacccg
     8761 tagtcgcaga catgcgcgtg gatgctgtcg gccagcgccc gccagcgcgc cgcgtcccct
     8821 tcgaatgtcg agttctcgat cagccgcgcg acgcggtcca cggccaccca caccatcacc
     8881 tgggaatggg tgtaggcgcc gacgtgcttg ccgcgttccc acaggcccgt accggctttc
     8941 tgccaatggt cctcgaggta ctcgaccatt ccgatctgct cgtcgaagtc ctcctggtgc
     9001 atcgcgaggc cgtgttcgtg cgccacgtgc acgccgttga tgacttcgcc gtagccgtcc
     9061 atctgggtct gccgccaggc cttgttgccg atgagcaccg gctggcttct ctcgtacccg
     9121 cgaaggtggt gcgccttctg ctgggtgatg cggcaggcgc catcgacgcg gtacaccggc
     9181 tgcaggcgcg ccggatcgcc ggcggtggta tcgatcagcc agttgcgcca catgcgtgct
     9241 tcgtcgtggt acccggaatg gatcattgcg tacaacgtga aggtggcgtc ccgcagccac
     9301 gtgtagcggt agtcgtaatt cttcacgcca ccggggatct cggggagtcc cagcgtcggc
     9361 gccgcgatga ccgcgccggt gcggtcgtcg ctcaacgact tcaacgtcag cagcgagcgc
     9421 agcacttcgt cgcgccacgg attggactgc atgtaacggc ggccccactt ccgccagaaa
     9481 cgctcggtct gccgcaatga ccggccggca tcacgctgcg ccgggccgtc gcgccacgcg
     9541 ttgtgccagg tcagcatgaa cgccacgcgc tcgccgcgcc tcacggtgaa gcgggcgcac
     9601 caggccccat cctcctgctc gagtgggggt ggtgaatgca attgcagcgc atcgggcccg
     9661 gcgacgatcc gcgcaccgtc gtcgcagcgc ctgcaccagg ggcgaatcct tccgtaatcg
//
LOCUS       DASSNH010000270         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_416779, whole genome shotgun sequence.
ACCESSION   DASSNH010000270 DASSNH010000000
VERSION     DASSNH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416779"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VFK00_09920"
     CDS             <1..121
                     /locus_tag="VFK00_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HET7593308.1"
                     /translation="RDAELGVLPMHDNRLHGMKKEKLLLKDRLTRMWSEIGTA"
     gene            227..1207
                     /locus_tag="VFK00_09925"
     CDS             227..1207
                     /locus_tag="VFK00_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7593309.1"
                     /translation="MALSTSIVLCTFNGARFLEIQWDSLLGQSRLPDEIVVRDDASTD
                     TTPDLLAGLKAKAASRGIAVQYIQGERNLGYVANFEMALGLASGDVLFLCDQDDRWHP
                     GKLAAQVEWFEQRPELLLLCGDARRIDADGATLPRTLFDVLRVSRGERQRIHAGGGFQ
                     VLLRRSLATGATIALRRELLAEALPFPEGWVHDEWLAIVAAALGGFDCMGMPLLDYRQ
                     HEANQIGMPERTMADKWVDLRRPRKALLDALIARGEVLDRRLSALAGKCPPEVGEQVV
                     RALAHWRARRALHDERWLRIGAIAREISNGCYRRYGSGWRSALRDFVRRA"
     gene            1300..>2142
                     /locus_tag="VFK00_09930"
     CDS             1300..>2142
                     /locus_tag="VFK00_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HET7593310.1"
                     /translation="MIVDSVLELIGHTPMVRVRRLDTGPCELFLKLESANPGGSIKDR
                     IGLSMIEGAEKAGKIKPGATLVEGTAGNTGLGLALVAQAKGYKLILVVPDKMSREKIF
                     NLKAMGAEVVLTRSDVAKGHPEYYQDLAAKIAEETPDAYFINQFGNPDNPLAHEQTTA
                     PEILEQMDGKLDAIVFGCGSSGTMTGLSHYFAEHSPDTELVLADPVGSILAEYINEGT
                     LSQKTASWMVEGIGEDFLPTISDFSRVKKAYSISDKESFLTARELLKQEGILGGSSTG
                     TLLAA"
BASE COUNT          394 a          652 c          725 g          371 t
ORIGIN      
        1 ccgcgatgcg gaactcggcg tactgccgat gcacgacaat cgactgcatg gcatgaagaa
       61 ggaaaagctg ctgctcaagg atcgcctgac ccgaatgtgg agcgaaatcg gcaccgcttg
      121 aagcgctgcg aacttccgaa aagaattccc gccggcccgc catgcgcggg ccggtttgcg
      181 tttgaggctt tgaagtctgc gcgggcgcgg cgacaatcgc gcggtcatgg cgctgtcgac
      241 atccatcgtc ctgtgcacct tcaacggtgc gaggttcctc gaaatccaat gggacagcct
      301 gcttggccag tcgcggctgc cggacgaaat cgtcgtgcgc gacgatgctt cgaccgacac
      361 caccccagat ctgctggcgg gcctgaaggc gaaggccgcg tcgcgcggga tcgccgtgca
      421 gtacatccag ggcgagcgca acctgggcta cgtcgccaac ttcgagatgg cgctagggct
      481 ggcgtccggt gatgtgctgt tcctgtgcga ccaggacgat cggtggcatc cgggcaagct
      541 tgctgcgcaa gtggaatggt tcgagcagcg acccgaattg ctgttgctat gcggcgacgc
      601 gcggcgcatc gatgccgatg gcgccaccct cccgcggacc ctgttcgatg tgttgcgcgt
      661 ctcgcgcggc gagcggcagc gaatccatgc cggcggtgga ttccaggtgc tgctgcgccg
      721 gagcctggcg acaggcgcga cgatcgcgct gcggcgggaa ttgctggccg aagcgttgcc
      781 gtttccggaa ggctgggtgc acgacgagtg gcttgcgatc gtcgcagcgg cactgggcgg
      841 cttcgactgc atgggaatgc cgttgctcga ttaccggcag cacgaagcga accagattgg
      901 catgcccgaa cgaacaatgg ccgacaagtg ggttgacctg cggcgccccc gaaaagcgct
      961 gttggacgca ctgatagccc gtggcgaggt gctggatcgg cgactctcgg cgcttgcagg
     1021 caagtgtccg ccggaggtcg gcgagcaggt cgtgcgggcg ctcgcacatt ggcgtgcgcg
     1081 acgagcgttg cacgacgagc ggtggttgcg gatcggcgcc atcgcgcgcg agatatccaa
     1141 tggctgctac cgccgctacg gctctggctg gcggtcggcg cttcgcgatt tcgtccggcg
     1201 cgcctgaatc ggatggttac ccgccgctat catgcggaag gaagtggatt cggacgcgat
     1261 gcggcacgtc cgtccggttg atcttgactc ggagccaaca tgatcgtcga cagcgtcctc
     1321 gaattgatcg gccacacgcc gatggtgcgc gtgcgccggc tcgataccgg gccgtgcgag
     1381 ttgttcctga agctcgaatc ggcgaatcca ggcggttcga tcaaggatcg catcggcctg
     1441 tcgatgatcg aaggcgccga gaaggccggg aagatcaagc ccggcgccac gctggtcgag
     1501 gggaccgccg gcaataccgg cctcggcctc gcgctggtgg cgcaggccaa gggctacaag
     1561 ttgatcctcg tggttccgga caagatgagc cgcgagaaga tcttcaacct gaaggcaatg
     1621 ggcgccgagg tggtgttgac ccgctccgac gtcgccaagg gccaccccga gtactaccag
     1681 gacctcgccg cgaagatcgc cgaagagacc ccggacgcgt acttcatcaa ccagttcggc
     1741 aatcccgaca acccgttggc ccacgagcag accacggcgc cggaaatcct cgagcagatg
     1801 gacggcaagc tcgacgcgat cgtcttcggc tgcggttcgt ccggcaccat gacggggttg
     1861 tcgcactatt tcgccgagca ctcccccgat acggaactgg tgctggccga cccggtcggt
     1921 tccatcctcg ccgagtacat caacgagggc acgctttcgc aaaagacggc gtcctggatg
     1981 gtggaaggca tcggtgaaga tttcctgccg accatcagcg atttctcgcg ggtgaagaag
     2041 gcctactcga tcagcgacaa ggaaagtttc ctgaccgcgc gcgaactcct gaaacaggaa
     2101 gggatcctgg gcggttcgtc gacaggaacg ttgctcgccg cg
//
LOCUS       DASSNH010000271         5315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1421034, whole genome shotgun sequence.
ACCESSION   DASSNH010000271 DASSNH010000000
VERSION     DASSNH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421034"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFK00_09935"
     CDS             complement(<1..444)
                     /locus_tag="VFK00_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593311.1"
                     /translation="MAGRGFAITAALLSLPALAGPPYITDDPEPTGTGHYEIYFFSEG
                     ASARDGREGSAGIDFNYGAADNLQLTATLPIAWSAVAGGSTAGIGNIELAAKYQFLHQ
                     RGAGVDAAFFPRVFLPAAPSVVAEDHASLLLPLWLQRSWGAWTVFG"
     gene            complement(509..2224)
                     /gene="dnaG"
                     /locus_tag="VFK00_09940"
     CDS             complement(509..2224)
                     /gene="dnaG"
                     /locus_tag="VFK00_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7593312.1"
                     /translation="MPGLIPERFIDELLSRVDIVDVVQQRVPLKRAGREWTACCPFHD
                     ERTPSFTVSPAKQFYHCFGCGAHGSAIRFLMDYDRLEFPDAVEELAQAVGVTVPHEGG
                     PARPREDRTDLYALLDGAATFYQDQLPKSEAARAYCKKRGLDKDIVQRFRIGWAPGGW
                     DAVKRALGTSDARVKMLEQAGMLSTGDKGKYDRFRERLMFPIMDRRGRVIAFGGRILE
                     GDGPKYLNSPETPLFHKGRELFALWQVRQANTKLERLIVVEGYMDVVSLHQAGVAQAV
                     ATLGTATTGEHAELLFRTAPDVYFCFDGDRAGRSAAWRALESVLPRMRDGRQAFFLFL
                     PEGEDPDSLVRKEGSAGFEARLRQATPLSEYFYGFLSHDVNVATLDGRARLAEHAKPL
                     LAKLPDGAFRDLMYAELEKRTGVQTAPAMPVRLPSASPGTPRRTLVRSAIAMLLAQPA
                     LAQEVTLPYAFAELDKPGIPLLAELLDFLQARPGVNTAMLLEHFAGREESAALQKLAL
                     TEFPGEPETLRTEFLDALRKLAEQTTQQRLDALIRKQSEGALDANDKNELRELLARKA
                     NPNPA"
     gene            2391..2867
                     /locus_tag="VFK00_09945"
     CDS             2391..2867
                     /locus_tag="VFK00_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593313.1"
                     /translation="MRRLCLLMVLPLSMWAWPAPARAGSYADTLSQCLIHATTPADRK
                     IALRWAFATMALDPDVASMASITTVQRDAINRQAGVLVTDLLVNACRQPVQQALMFEG
                     PPAVETAFETWGRWAVTGMVTEPHVARGMSALLKYLDMGKLMSLVPLQGFPPAGRS"
     gene            2985..3944
                     /gene="cysK"
                     /locus_tag="VFK00_09950"
     CDS             2985..3944
                     /gene="cysK"
                     /locus_tag="VFK00_09950"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7593314.1"
                     /translation="MSLYDSIVHTIGDTPIVKLHRLPPAHVTLYAKIESFNPGGSVKD
                     RLALAVILDAEQRGELEPGQTVVEATSGNTGVALAMVCAARGYPFVAVMSDSFSIERR
                     KLMRAYGARVILTPAAERGRGMVRKAEELAKRHGWFLTRQFENEANPAWHRNTTGPEI
                     LRDFAGQRLDYFVSGWGTGGTLTGAGEMLKRARPDLKVIATEPANASLLAGGEWHSHK
                     IQGWTPDFLPKVLSRTIADEIIPIDEVLARDTARRLAQEEGIFCGISSGATVAAALEA
                     AQQAPQGSVLLAMLPDTGERYLSTFLFEGVDEGSDDDWLNQQG"
     gene            complement(3966..4235)
                     /locus_tag="VFK00_09955"
     CDS             complement(3966..4235)
                     /locus_tag="VFK00_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7593315.1"
                     /translation="MSDDVQPMVFSIIAREAAIDEDGVELDATLKDLGIRSLDRAQIV
                     FAIEDHFRIQIPDRDPDFDTRSVSGLVDAVEKLLAEQAAHPSASS"
     gene            complement(4347..4796)
                     /locus_tag="VFK00_09960"
     CDS             complement(4347..4796)
                     /locus_tag="VFK00_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7593316.1"
                     /translation="MSELKARLTEDMKTSLKGGDKARLAAIRLVLAAIKQREVDERIT
                     LDDAQVLHVLEKMLKQRRDSVAQYEAAQREDLAEQERYEIGVIEAYLPAQLSDAELDT
                     LVQACIVDAGANSPRDMGKVMALLKERAAGRADMGALSQRVKARLAS"
     gene            complement(5042..5257)
                     /gene="rpsU"
                     /locus_tag="VFK00_09965"
     CDS             complement(5042..5257)
                     /gene="rpsU"
                     /locus_tag="VFK00_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7593317.1"
                     /translation="MPSVKVRENEPFEIALRRFKRTCEKAGVLAETRKREYYEKPTQE
                     RKRKRAAAVKRHLRRVSREVTRRKRLY"
BASE COUNT          837 a         1763 c         1778 g          937 t
ORIGIN      
        1 gccgaacacc gtccacgcgc cccagcttct ttgcagccac agcggcagga gcagcgaggc
       61 gtgatcttcc gcaacgacgg acggggccgc cggcaggaac acgcgcggga agaacgccgc
      121 gtccacgccc gcaccccgtt ggtgcaggaa ttggtatttc gccgccagtt cgatgttgcc
      181 gatgccggcc gtgctgccac ctgccaccgc gctccatgcg atcggcaggg tggcggtgag
      241 ctgcaggttg tcggctgcgc cgtaattgaa atcgatgccg gccgatcctt cgcggccatc
      301 acgcgccgaa gcaccttcgc tgaagaaata gatttcgtaa tggccggtgc cggtcggttc
      361 gggatcgtcg gtgatgtacg gcggccccgc caacgccggc aacgacagca gcgctgccgt
      421 gattgcgaac ccgcgcccgg ccatgcgccg aatccgcgtc atgcttgcgg cggcatccgg
      481 ctgccatgcg ggtggcgggt cgcggtggtc atgccgggtt ggggttggcc ttgcgcgcca
      541 gcaattcgcg caattcgttc ttgtcgttcg cgtccagcgc gccttcggac tgtttccgga
      601 tcagggcatc caatcgttgc tgggtggtct gttccgccag cttgcgcagc gcgtccagga
      661 actcggtacg cagcgtttcc ggttcgcccg ggaattcggt caacgccagt ttctgcagcg
      721 cggcggattc ctcgcgtccg gcaaaatgtt cgagcagcat cgcggtgttg acgccggggc
      781 gggcctgcag gaagtccagc agttcggcca gcagcggaat gcccggtttg tccagctccg
      841 cgaacgcgta cggcaaggtg acctcctgcg ccagcgccgg ctgcgccagc agcatcgcga
      901 tcgcgctgcg caccagggtt cgccgcggcg tgccgggcga ggcggaagga agacgtacgg
      961 gcattgccgg tgcggtttgc accccggtgc gcttctcgag ttccgcgtac atcaagtcac
     1021 ggaacgcgcc atccggcaat ttcgcaagca ggggcttcgc gtgttcggcc agccgcgcgc
     1081 gcccgtccag ggtcgcgacg ttgacgtcgt gcgacagaaa gccgtagaaa tattccgaca
     1141 gcggcgttgc ctgcctgagc cgtgcctcga acccggcgct gccttccttg cgcaccagcg
     1201 agtcgggatc ttcgccctcc ggaagaaaga ggaagaatgc ctgccggccg tcgcgcatcc
     1261 gcggcagcac cgattccagt gcgcgccaag cggcgctgcg cccggcgcgg tcgccgtcga
     1321 aacagaaata gacgtcgggt gccgtgcgga acagcaattc ggcgtgctcg ccggtggtcg
     1381 cggtgccgag cgtcgcgacg gcctgcgcaa cgccggcctg gtgcagcgac accacatcca
     1441 tgtagccttc gaccacgatc aggcgctcga gcttggtatt cgcctggcga acctgccaca
     1501 gcgcgaacaa ttcgcggccc ttgtggaaca gcggcgtctc gggtgaattg aggtacttgg
     1561 gcccgtcgcc ctccaggatc cttccgccga aggcgatcac ccgtccgcgc cggtccatga
     1621 tcgggaacat cagccgttcg cggaaacggt cgtacttgcc cttgtcgccg gtcgagagca
     1681 tcccggcctg ttccagcatc ttcacccgcg cgtcgctggt gccgagcgcg cgcttcaccg
     1741 catcccagcc gcccggggcc cagccgatcc ggaaacgctg gacgatgtcc ttgtccaggc
     1801 cccgcttctt gcaataggca cgtgccgctt cgcttttcgg caactggtcc tggtagaagg
     1861 tcgcggcgcc gtcgagcagc gcgtagagat cggtgcggtc ctcccgtggc cgggcgggcc
     1921 cgccttcgtg cgggaccgtg acgccgaccg cctgggcgag ttcctcgacc gcgtcgggaa
     1981 actccaggcg gtcgtaatcc atcaggaacc tgatcgcgct gccgtgggcg ccgcatccga
     2041 agcagtggta gaactgcttc gcggggctga ccgtgaacga gggcgtgcgt tcgtcgtgga
     2101 aagggcagca cgccgtccac tcgcgtccgg cacgcttcag cggcacgcgc tgctgcacca
     2161 cgtcgacgat gtcgacgcgg ctcagcagct catcgatgaa gcgttcgggg ataaggccgg
     2221 gcatgacccg ttagcatgcc acgccccgac agcgcacgcg acgagcgcct gaccgcacag
     2281 gcgcagggcc ccgcaccgcc accgttgggc gccgccgcag ggtaggcgat ttcctgaacc
     2341 tgcacgcggc ggcgtggtcc catgggaacc ccggcgaaaa cgaacgcatg atgcgacgcc
     2401 tctgcctgtt gatggtgctg ccgctgtcga tgtgggcgtg gcctgcgccg gcgcgcgcgg
     2461 gttcctatgc ggacacgctc tcgcaatgcc tgatccacgc gaccacgccg gccgaccgca
     2521 agatcgcctt gcgctgggcc ttcgcgacga tggctctgga tcccgatgtc gcgtcgatgg
     2581 cctcgatcac aaccgtccag cgggatgcca tcaaccggca ggccggcgtc ctggtgaccg
     2641 atttgctggt gaatgcgtgt cggcagccgg tgcagcaggc cttgatgttc gaggggccgc
     2701 ccgcggtcga gacggcgttc gaaacctggg gccgctgggc cgtcaccggc atggtgaccg
     2761 aaccgcacgt ggcgcgcgga atgagcgccc tgctgaagta cctcgacatg ggcaagctga
     2821 tgtcgctggt gccgctgcag ggctttccgc cggcgggccg tagctgatgc gctgtccccg
     2881 aatcacgatg tcagtctttt tccggacggc actaacgcat tcctaatccg ccccgtccgc
     2941 gcggcgatta cgcttggctg ccctgcgacg aatggaatcg caccatgtcc ctgtacgaca
     3001 gcatcgtcca taccatcggc gacacgccga tcgtcaagct gcaccgtctc ccgcccgcgc
     3061 atgtcacgct ctacgccaag attgaatcgt tcaatcccgg cggttcggtc aaggatcgcc
     3121 tggcgctcgc ggtgatcctg gatgccgaac agcggggcga gctggagccc gggcaaaccg
     3181 tggtcgaggc gacgtccgga aacaccgggg tggcgcttgc gatggtgtgc gccgcgcgtg
     3241 gctatccgtt cgtggcggtc atgtccgatt cgttttcgat cgaacggcgc aagctgatgc
     3301 gcgcgtacgg cgccagggtg atcctcacgc ccgccgcgga acgtggccgc gggatggtgc
     3361 gcaaggccga ggaactggcg aagcggcacg gctggttcct cacgcgccag ttcgagaacg
     3421 aagccaaccc ggcctggcat cgcaatacca ccggtccgga gatcctgcgc gactttgcgg
     3481 gacagcggct ggattatttc gtcagtggct ggggcaccgg cggtacgctt accggcgccg
     3541 gcgaaatgct gaaacgcgcg cgcccggacc tgaaagtgat cgcgaccgaa cccgccaacg
     3601 catccctgct ggccggcggc gaatggcact cgcacaagat ccagggctgg acgccggatt
     3661 tcctgccgaa ggtgctgagc cgcaccatcg ccgacgaaat catcccgatc gacgaggtac
     3721 tggcacgcga cacggcgcgc cgcctcgcgc aggaggaagg catcttctgc ggcatctcgt
     3781 ccggcgccac ggtggccgcc gcgctggaag ccgcgcagca ggcaccgcag ggttccgtgc
     3841 tgctggccat gctgccggac accggcgaac gctacctttc gaccttcctg ttcgaagggg
     3901 tggatgaagg cagcgatgac gactggttga accagcaggg ttgacgagcg cgggcggctg
     3961 gcctttcagc tgctcgccga aggatgcgcg gcctgctccg ccagcagctt ttcgacggca
     4021 tcgacgaggc cgctgaccga tctggtatcg aagtcgggat cgcggtcggg aatctggatc
     4081 ctgaagtgat cctcgatcgc gaagacgatc tgggccctgt cgagcgatcg gatcccgagg
     4141 tccttcaagg tcgcgtccag ttccacgccg tcctcgtcga tggcggcctc ccgggcgatg
     4201 atggaaaaaa ccatcggttg gacgtcatcg ctcatggtgg ctcccttcgc ggtggatggg
     4261 cgccggcgcg ggcggcgcgg ccgcgcgccg ctggggagcg tcaggatacc agtcgtgatc
     4321 gttgccccgg cgtggccacg gccgcgtcag cttgcgaggc gcgccttgac ccgctgtgac
     4381 agcgcgccca tgtcggcgcg cccggcggcg cgctccttca ggagcgccat caccttgccc
     4441 atgtcgcgcg gcgagttggc gcccgcgtcc acgatgcacg cctgcaccag cgtatcgagc
     4501 tcggcatcgg agagctgcgc cggaaggtaa gcctcgatca cgccgatttc gtagcgttcc
     4561 tgttcggcca ggtcctcgcg ctgcgcggcc tcgtactgcg cgacggagtc gcggcgctgc
     4621 ttgagcatct tttccagcac gtgcaggacc tgcgcgtcgt ccagcgtgat gcgttcatcg
     4681 acctcgcgct gcttgatcgc ggcgagcacc agccggatcg ccgcgagccg cgccttgtcg
     4741 ccgcccttca gcgaggtctt catgtcttcg gtaaggcgtg ccttcaattc gctcattggg
     4801 acatctcgat gggtcagtgg agcgcttcga caagcacgag gctttaccga ggctttcccg
     4861 actgtacgac aaaagcctct gacgcggatg gacgcggatc aatcatcttc atcggtattc
     4921 atccgcgttg atccgcgtcg gaaccgttct tttgcattgc caggaagcac cgcgatacaa
     4981 acgcaaaaag ccggtgcagc agggcagcac cggctcgtgg atcctgtcgt cgtggagcgg
     5041 ctcagtacag gcgcttgcga cgcgtgactt cgcgcgagac gcggcgcaga tgccgcttca
     5101 ccgccgcggc gcgcttgcgc ttgcgctcct gggtcggctt ctcgtagtac tctcgcttgc
     5161 gcgtttcggc gagtacgccg gccttctcgc aggtgcgctt gaagcggcgc agggcgattt
     5221 cgaacggctc gttctcgcgg actttgacgc tgggcatgga cgctccactt ggtaaactgg
     5281 cccgcttggc cgcgggtgag ccgcgaatta tagcg
//
LOCUS       DASSNH010000272        11097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1421562, whole genome shotgun sequence.
ACCESSION   DASSNH010000272 DASSNH010000000
VERSION     DASSNH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421562"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFK00_09970"
     CDS             <1..276
                     /locus_tag="VFK00_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HET7593318.1"
                     /translation="EIQGLQVKALESQAKSNLAFVADSLEAREIQDVTALWPKGIDFA
                     RYTAEAAYATGQEILGVAQKTAEQFGDLTRNSLKAANDAAAPAKKAR"
     gene            complement(495..1334)
                     /locus_tag="VFK00_09975"
     CDS             complement(495..1334)
                     /locus_tag="VFK00_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593319.1"
                     /translation="MKYCIFTILWLVAGLLAMPNLARAVCDQTGASQADRDLWATHGC
                     WQDFFLWQYRAYDMNGDDWANRGWNDACNQNLEYPKHWNASYLVTYGLIDSNAQSFHG
                     TIDYRATAEAASSNFHNHLRHVPTDRSGIFGSYSDGKVATACPLYDPAVANANPASRG
                     GDFMHEGWHAYFDKYGWDNGSVGGHRPKQGNCTVDGCDYFYFHGIGAYAFGAMWQNDG
                     TANRFHSPNQVQVEYLCDVADQPKAWVPNSVRLAAQNDANTRAGERFINGPGYRCGDP
                     RPW"
     gene            complement(1331..2230)
                     /locus_tag="VFK00_09980"
     CDS             complement(1331..2230)
                     /locus_tag="VFK00_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593320.1"
                     /translation="MRRHFSSRIVILSVVGLILGGCMTKEPGGTRPAGPGPTASTPSS
                     PAPDVLRAGLAHQPLPQLVDASDVQAFVVAAGAAPVARREEIRAIIDGAHDNTEITKS
                     LIREFETAQTTDFSRALVLLSLIGEQRNPAGVAFLVDFVWQPLPKGGPVIEELGMSAE
                     AEAMERLQVKAANAIPYARTEKALQAALEIAAKHPLKTVRIEAASSYLWNQRNSDQAR
                     RALAAVLRKDDLVVLDRPVHDTGMSAREFNSQIANYLDRHPELQPPAPRRTGRGKPGP
                     ATERTTLPPPPSESSSAALEKSQ"
     gene            complement(2347..2943)
                     /locus_tag="VFK00_09985"
     CDS             complement(2347..2943)
                     /locus_tag="VFK00_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7593321.1"
                     /translation="MFTEYHSEALAVWAFRSGVWDYRVKPLDVESFTRSIEVLVELVQ
                     PHPLGRTCEGSLPPDLIEPAGHLRRPPATARKSGAAVAYIAVHFDQAVKRDMLARLCH
                     LSRSEFSRVFRREQGMTFEHFLLEYRIAQARELLGEPSSTVAQAAYASGFRDPSYFSR
                     TFHRLVGVTASEYRQRATARHIESFAPPGAQSSSDHAR"
     gene            complement(3407..4246)
                     /locus_tag="VFK00_09990"
     CDS             complement(3407..4246)
                     /locus_tag="VFK00_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7593322.1"
                     /translation="MASRNVTRRVRGIPTSDGAGVKLTRVIGQPKLDSLDPFLLLDEF
                     GSEAATDYIGGFPDHPHRGFETVTYMLDGRMRHRDNHGNSGLLESGSVQWMTAGRGIV
                     HSEMPEQEHGLMRGFQLWVNLAARDKMIEPRYQDIAPDRIPIVEPAPGVTVRVVAGDL
                     FGVTGPVSGIAIEPVYLDAGLQPGAKLSVPLPEGHSAFAYVFEGETVQVGDDTLGLRE
                     LGVLSPGDQVELSVASDAPAARVLLVAGRPLNEQVARYGPFVMNTPEQIVQAMQDYQA
                     GRF"
     gene            complement(4417..5097)
                     /locus_tag="VFK00_09995"
     CDS             complement(4417..5097)
                     /locus_tag="VFK00_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HET7593323.1"
                     /translation="MDVQPAPREIIDDAELEVLDRFLRGHAGDSDETMLLDGVHGMLT
                     ALAIGPDPALPEEWLPEVLHAPFEDPEQGAAVLGLLAKLNDSIPVELEGEVYEPVLGE
                     LGDGEPLHSDLSAAGWCEGFSRGIDLRAAIWESRLAEDSELLEMLGPIMALAVDEGVL
                     SADVPIAKLDDNEYDACLAQIPDAVEAVAHYWRVRPPTERERTSGGGASAASFPSLPS
                     RRRGRWLH"
     gene            complement(5123..5890)
                     /gene="xth"
                     /locus_tag="VFK00_10000"
     CDS             complement(5123..5890)
                     /gene="xth"
                     /locus_tag="VFK00_10000"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7593324.1"
                     /translation="MRIASWNVNSLKVRLPHLEQWCREAQPDVMALQETKVGDDKFPR
                     EAIEALGYQVVFSGEKAYNGVAILAREPLLDAVAGVPEFGDPQRRVLAATIAGVRIVN
                     LYVVNGQSAGSDKYAWKLEWLQAVTGYLRGELQRHGQVIVLGDFNIAPEDRDVHDPEA
                     WRGQVLCSEPERAALRGILDLGFIDSFREFVADPGHFSWWDYRQGAFRRNFGLRIDLI
                     LASEALRGRLRAASIDGAPRRWERPSDHAPVILELAN"
     gene            6086..6802
                     /locus_tag="VFK00_10005"
     CDS             6086..6802
                     /locus_tag="VFK00_10005"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HET7593325.1"
                     /translation="MLATDLLLLALDDERGTVLPEAGIGLDYGLAGAVVMELALRGRL
                     QVDGERVTATGTATDDALLDGALRAVAATPGKTLSHWVRHLPGELNGLRQRLLDRLVA
                     RGTLAKREQRVLLLFHRNVYPERDSRVEHDVRARLDGVLLHGEPPDAATACLIHLAAA
                     CRVTDAIYPRDQHKAIRARMADLDDAGAAGANAVADMVARAETAAVTAAAMSAITAST
                     IAATTAATTAACSASTVVCT"
     gene            complement(6882..8945)
                     /locus_tag="VFK00_10010"
     CDS             complement(6882..8945)
                     /locus_tag="VFK00_10010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7593326.1"
                     /translation="MSNDNPLLGDAQLPAFSKIRAADVEPAIDAILAHYQAGIDLLTA
                     AASPRDFEHVMLESERLDQRLARAWAPVSHLHAVADTRELRAAYDAALEKITDFESAL
                     GQNRDLYAAVQAVADAPGFAARPRPERALVEHALRDFRLSGVALEEPARTRFREIVNE
                     LAKLTTGFANAVLDASEAWYERIEDERDLSGIPESGRAVLREYARERELEGSLVTLKQ
                     PAVQAVLTYADNRGLRERVYWAYQTRASDQGPNAGKFDNSARMEKILALRHEAAQLLG
                     FANAAEESLATKMATTPDEVLTFLRDLAARAKPVAQRELDELREFARSELGLETLEPW
                     DVAYASEKLRRARYALDEEQLKAYFPLPSVLDGLFTVAGKLYGISVESAREPVDVWHP
                     DVRYCEVRDADGALIAGVYLDLHARDGKRGGAWMDVCRARFRDGDRTQLPIAFLTCNF
                     APPAGGHPSLLTHDDVLTMFHEFGHGLHHLLTRIELPSIGGIDGVEWDAVEMPSQFME
                     NFCWNRRALDLFARHWSTGEPLPGELFDNMLAARHFQAGMFLVRQLEFALFDFLLHLE
                     YRPDRGARVMETLEAVRREVAVLHPPAWQRFPHAFTHIFAGGYAAGYYSYLWAEVLSA
                     DAFGAFDERAGETGEVIDAATGARFRDEVLGVGASRPALESFIAFRGRAPQPDALLKS
                     YGLDA"
     gene            9009..10808
                     /locus_tag="VFK00_10015"
     CDS             9009..10808
                     /locus_tag="VFK00_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7593327.1"
                     /translation="MRTTPAVDLRALLAATAVFGHLDRGELGALLEEMAWFLLPGGAT
                     LYQRGEEPDALFLLKSGSLGLFGTPLIGGPARLLGVIPAGETLGEIGLLVGEERTHSV
                     RALRDSELLRLSREGFEKLVARHPQAMLSAARVAIQRLLGDPHEEATSPPRTFAVLPF
                     DHEIDAHAFAESLQHQLLAWGDCLVIDAGLGRSRDTGWFTERESEHRFVLYLGDGDPT
                     WRRLCQRQADALLLLVAAERTPEPWPYPVGNRPAADAGHPQSHEAMVEDTHHRPRHLV
                     LQHPRGQFIYGAARAWRASIGTAELQHHHWRHHGDMARIARLLSGNSTALVLSGGGAR
                     GFAQIGVAQALREAGISVDTVVGTSIGAIIGAGIAYEWDDGKLRAACRRALVDGKPLA
                     DWTLPLVALTRGARATMLMREAFGEIDVEDLPVPFACVSADLTDGATAVHREGLLWRW
                     IRASSAVPGILPPLFHDHHVYVDGAVINNLPTDVLAEDAPGRMIAVDIGADDTLRAEV
                     NEAASPRWPTLWAERRTRQRRPGAFAILIRAGMVNSETGSARRRELADLVLRPDMQEI
                     GMFDWHHFDRAIEIGYRSTRDALREHTAPTGKS"
     gene            10914..>11097
                     /locus_tag="VFK00_10020"
     CDS             10914..>11097
                     /locus_tag="VFK00_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7593328.1"
                     /translation="MANLRAYRGVEPRLGARVYVDPAATVIGDVELGDDVSLWPGAVV
                     RGDVEKIRIGARSNVQD"
BASE COUNT         1830 a         3773 c         3709 g         1785 t
ORIGIN      
        1 gaaatccagg gcctgcaggt caaggcgctg gagtcgcagg ccaagtcgaa cctggcgttc
       61 gtcgccgatt cgctggaagc ccgcgagatc caggacgtga ccgcgctgtg gcccaagggc
      121 atcgatttcg cgcgttatac cgccgaagcc gcctacgcga ccggccagga aatcctgggc
      181 gtcgcgcaga agaccgccga gcagttcggc gatctgaccc gcaacagcct caaggcggcc
      241 aacgacgccg ccgcgcccgc caagaaggcg cgctgattcc gcaagggatc gaacgaggta
      301 cgcgcggcgg agacccccct ccctcctgca gcgcgactcc ggccggacgg cgcaagccgt
      361 ccggcttttt tcatgcgcag gagaaaacgc tcgacaaatc cctcaggcgc ggtcaccgca
      421 atcggtccgg cgcgcacggg ctcacgagct gcaggaggct cgacgttcgg gcggcggacg
      481 gtggtgccgc cctttcacca cggccgcgga tccccgcatc gataaccggg gccgttgatg
      541 aaacgctcgc cagcgcgcgt gttggcatcg ttctgtgccg cgaggcggac cgagttgggc
      601 acccaggcct tgggttggtc ggcgacgtca cacaggtact cgacctggac ctggttgggt
      661 gaatggaagc ggttggcggt gccatcgttt tgccacatgg cgccgaaggc ataggcgccg
      721 atgccgtgga aatagaaata gtcgcacccg tcgaccgtgc aattgccttg cttgggccga
      781 tggccgccta cgctgccgtt gtcccaacca tatttgtcga aataggcgtg ccagccttcg
      841 tgcatgaagt cgccaccgcg cgaagcggga ttcgcattcg cgacggcggg atcgtagagc
      901 gggcatgcgg tggcgacctt gccgtcgctg taggacccga agatgccgct ccggtcggtg
      961 gggacgtgcc gcaggtgatt gtggaaattg ctcgaggccg cttccgcggt cgcgcgataa
     1021 tcgatcgttc cgtggaagga ttgcgcattg ctgtcgatca ggccgtaagt caccagatag
     1081 gacgcattcc agtgcttggg atattccagg ttctggttgc acgcatcgtt ccagcctcga
     1141 ttggcccagt cgtccccgtt catgtcgtag gcgcggtact gccacaggaa gaaatcctgc
     1201 cagcagccat gggtcgccca caggtcgcga tcggcctggc ttgcacccgt ctggtcgcat
     1261 acggccctgg cgaggttcgg catcgccagc aagcccgcga ccagccacaa aatcgtgaag
     1321 atgcaatact tcactggctc ttctccaggg ccgccgacga agattcggat ggcggcggcg
     1381 gcagcgtcgt ccgctcggtc gccgggcccg gcttgccgcg acccgtccga cggggcgccg
     1441 gtggctgcag ttcggggtgg cgatccagat agttcgcgat ctggctgttg aattccctgg
     1501 cgctcatgcc ggtgtcgtgg accggccggt caagcaccac gaggtcgtct ttgcgcagga
     1561 cggcagccaa tgcccgtcgc gcctggtcgc tgttgcgctg gttccacaga taactggacg
     1621 ccgcctcgat gcggaccgtt ttcaggggat gtttggcggc gatttccagc gccgcttgca
     1681 acgccttttc ggtgcgggca tagggaatcg catttgcggc cttcacctgc aaccgctcca
     1741 tcgcctcggc ctcggcggac atgccgagtt cctcgatcac cggcccacct ttcggcaggg
     1801 gctgccatac gaaatcgacc aggaaggcga cgccggcagg gttccgctgc tctccgatca
     1861 acgaaagcag gaccagggcc cgcgagaagt ccgtcgtttg cgcggtttcg aactcgcgaa
     1921 tgaggctctt ggtgatctcg gtgttgtcgt gcgcgccgtc gatgatcgcc cgaatctctt
     1981 cgcggcgcgc gaccggtgcg gcgcctgccg cgaccacgaa ggcctggaca tcggacgcgt
     2041 cgacaagctg cggtaatggc tggtgcgcga gtcccgcccg caatacgtcg ggtgcgggac
     2101 tggaaggcgt ggaggccgtc gggccaggac ccgccgggcg ggtgccccca ggctccttgg
     2161 tcatgcagcc gcccaggatc aagccgacga cgctcaggat cacgatgcgc gaagaaaaat
     2221 gacgccgcat ggctcggcct cgattcctgg tgttcgcgaa acgatacgtg tctgtcagca
     2281 gtttcggcca atcaccgccc gtacgtggag ggaagtgtat tgcgggccca agcgggcccg
     2341 tttgcactat cgtgcgtgat cgctggagga ttgtgcgccc ggaggagcga acgattcgat
     2401 gtgccgtgcc gttgcgcgct gccggtattc cgatgccgtc acgcccacca gccgatgaaa
     2461 cgtccgggaa aaatacgacg gatcgcgaaa gccggaggca taggcagcct gtgcgaccgt
     2521 cgaactcggc tcccccagca actcacgcgc ctgggcaatc cggtattcga gcaggaagtg
     2581 ttcgaaagtc attccctgtt cgcgccggaa tacgcgactg aactcggaac gactgaggtg
     2641 gcagagacgt gccagcatgt cgcgcttcac ggcttggtcg aaatgaactg cgatgtaggc
     2701 aacggccgct cccgatttcc gtgccgtcgc aggcggacgc cgcaagtggc ccgccggttc
     2761 gatgaggtcc ggcggcaacg acccttcgca agtgcgacca agggggtgag gctgtacgag
     2821 ctcgaccaac acctcgatgc tgcgcgtaaa ggactcgaca tccagcggct tgacgcgata
     2881 gtcccagacg ccggagcgaa acgcccagac cgccagcgcc tcggagtgat actcggtgaa
     2941 catgagcaac gggagccccg aaaacacccg cctgagcgag gtcagggtcc ccaatcgcat
     3001 ccgatccgga taatcgaact cgatgcacgc gaacttcggg cggaaatcac gcaccgcctc
     3061 gagcgcgctc gcggatccgt cgatcctcct gacttcatgg tgccccggca cgagttgcgc
     3121 caatccgccc gtcaccggct gcctgcacag atcgatccac agaatgcgaa ccgctcccat
     3181 cgaggcgcac tcgtgttttc acgcggccgg cgcgcaggcc gaacccgtcg ccgcccatac
     3241 cgcgttttca tggcaggggc atcgacggca ccggcgcacg cacgccttct tgtatgcgcg
     3301 cgccgcggta ccggattgta gagccgcgat ggcaggaacg gggtcaggga caacgttggc
     3361 gtgaagcgcg cggtgctggt gcagcaggca cttgcccctg acgccctcag aaccttccgg
     3421 cctggtaatc ctgcatcgcc tgcacgatct gctcgggcgt gttcatcacg aacggaccgt
     3481 agcgggcaac ctgttcgttg agtggccggc cggcgaccag cagcacgcgg gcggccggcg
     3541 cgtcggaggc aaccgagagt tccacctggt cgccgggcga caacacgccg agttcgcgca
     3601 agcccagcgt gtcgtcgccg acctgcacgg tttcgccttc gaacacataa gcgaacgcgc
     3661 tgtgcccttc gggcagcggc accgacagct tcgcgccggg ctgcaagccc gcgtcgagat
     3721 acaccggctc gatcgcgatg ccgctgaccg gtcccgtgac gccgaacaga tcgcccgcga
     3781 ccacgcgcac cgtgacaccc ggcgccggtt cgacgatcgg gatcctgtcc ggcgcgatgt
     3841 cctggtagcg cggttcgatc atcttgtccc gggccgccag gttcacccat agctggaagc
     3901 cacgcatcag gccgtgttcc tgctcgggca tttccgagtg cacgatgcct cgcccggcag
     3961 tcatccactg cacgctgccc gattccagca ggccggaatt gccgtggttg tcgcggtggc
     4021 gcatgcggcc atcgagcatg taggtgacgg tctcgaagcc gcgatgcggg tggtcgggga
     4081 acccgccgat gtagtcggtc gccgcttccg atccgaactc gtccagcagc aggaacgggt
     4141 cgagcgaatc gagcttcggc tggccgatca cgcgggtcag cttgacgccg gcaccgtccg
     4201 aggtcgggat gccgcgcacg cgacgggtga cgttgcggga ggccatgggc cacctccgat
     4261 gggatcagat ggcttcaaag atgcgcccgc gcacgacggg aaacaatgcc ccccggggaa
     4321 acggtgcgtt cccgcaaagc gaagaacccg aagaagacca ttacgctttc cgagcccgaa
     4381 gctcgattca ccagtcccgg gccttggggc cgcctctcaa tgcaaccagc ggccccgccg
     4441 ccgcgaaggc aggctgggaa acgatgcagc cgacgcgcct ccgcctgagg tgcgctcgcg
     4501 ctccgtgggc ggacgcaccc gccagtagtg ggcgacggct tccacggcat cggggatctg
     4561 ggccaggcag gcgtcgtatt cgttgtcgtc gagtttggcg atcggcacgt cggccgaaag
     4621 cacgccttcg tcgaccgcga gcgccatgat cggacccagc atctcgagca gttcgctgtc
     4681 ttcggcgagc cgcgattccc agatcgcggc gcgcaggtcg atgccgcggc tgaacccctc
     4741 gcaccagccg gcggcgctca ggtcgctgtg gagcggctcg ccgtcgccga gctcgcccag
     4801 taccggctcg tagacctcgc cctcgagttc cacggggatc gaatcgttga gcttggcgag
     4861 cagcccgagt accgccgcgc cctgttccgg gtcctcgaaa ggcgcgtgca gcacctcggg
     4921 cagccattcc tcgggcagcg ccggatcggg cccgatcgcc agcgccgtca gcatgccgtg
     4981 caccccgtcg agcagcatgg tttcgtcgga atcaccggcg tggccgcgca ggaagcggtc
     5041 gagtacttcc agttcggcgt cgtcgatgat ttcccgcggc gcgggctgca cgtccatggc
     5101 ttccatgatc ggggcagacg ccctagttcg caagctcgag gatcacgggc gcatggtcgg
     5161 acggccgctc ccagcggcgc ggcgcgccgt cgatcgacgc cgcccgcagg cgcccgcgca
     5221 gggcttcgct ggcgaggatc aggtcgatgc gcaagccgaa gttgcgcctg aacgcgccct
     5281 gccggtagtc ccaccaactg aagtggccgg ggtcggcgac gaattcacga aacgagtcga
     5341 tgaagccgag gtcgaggatt ccgcgcagtg cagcgcgctc tggctccgag cacagcacct
     5401 ggccgcgcca ggcttcggga tcgtgcacgt cgcggtcttc cggcgcgata ttgaagtcgc
     5461 cgagcacgat gacctggcca tggcgctgca gctcgccgcg caggtaaccg gtgaccgcct
     5521 gcagccactc gagcttccac gcgtacttgt cgctgccggc gctctgtccg ttgacgacat
     5581 agaggttgac gatccgcacg ccggcgatcg tcgcggccag cacgcggcgt tgcggatcgc
     5641 cgaattcggg cacgccggcc acggcatcca gcaacggttc gcgcgcgagg atcgcaaccc
     5701 cgttgtaggc cttctcgcca gagaacacca cctggtagcc gagcgcctcg atcgcctcgc
     5761 ggggaaactt gtcgtcgccg accttggttt cctggagcgc catgacgtcc ggctgcgctt
     5821 cgcggcacca ctgctcgagg tgcgggagcc gcaccttcaa cgaattgacg ttccaggagg
     5881 cgattcgcat cgaaacattc tatgtgcgat catccctgat cgctgcagtt gccatcccct
     5941 tgcagcagac gattgacttc ggccaaccga catcaccacg gccatccacg gcctgacttt
     6001 ccacaacggc tgcttcgcca aaaccacccg tcgcagacga cggttaccca ggcacctttg
     6061 gcgaaatgct tccatggaga attccatgct cgccaccgac cttctgctgc tggccctcga
     6121 cgacgaacgc ggcaccgtgc tgccggaggc gggaatcggc ctcgattacg gactcgccgg
     6181 cgcggtcgtg atggaactcg ccctccgcgg acgcctgcag gtggatgggg agcgggtgac
     6241 cgcgaccggt acggcgaccg acgatgcgct gctcgatggt gcgctgcgcg cggtcgccgc
     6301 gacgcccggc aagacgctgt cgcactgggt ccggcatttg ccgggcgagt tgaacggttt
     6361 gcggcagcgc ctgctcgacc gccttgtcgc gcgagggacg ctcgccaagc gcgagcagcg
     6421 cgtgctgctg ttgttccacc gaaacgtgta tccggaacgc gactcccgcg tcgagcacga
     6481 cgtgcgcgcg cgactggacg gcgtgctgct gcacggcgag ccacccgatg ccgcgaccgc
     6541 ctgcctgatc cacctcgccg ccgcctgccg cgtcaccgat gcgatctatc cgcgcgacca
     6601 gcacaaggcg atccgcgccc ggatggccga cctggatgat gcaggcgcag ccggcgcgaa
     6661 cgcggtggcg gacatggtgg ctcgcgccga aaccgccgcc gtgaccgcgg cggcgatgtc
     6721 ggcgatcacc gcgtcgacga tcgccgcgac cacggctgcg accacggctg cctgttcggc
     6781 cagcacggtg gtttgcactt gaagcgcgag gcgaaccaat gcctgtcgcg cgcatgcgcg
     6841 ctcctacgac gatcgctccg gcgcatccgt gatcccgggc gtcaggcatc caacccgtag
     6901 gacttcagca acgcatcagg ttgcggtgcg cggccgcgga aggcgatgaa actctcgagc
     6961 gcaggcctgc tcgcgccgac gccgagcact tcgtcgcgga aacgtgcgcc ggtcgcggcg
     7021 tcgatcacct cgccggtttc cccggcgcgc tcatcgaagg caccgaacgc gtcggccgag
     7081 agcacctcgg cccacaggta gctgtagtac cccgccgcgt agccgcccgc gaagatgtgg
     7141 gtgaacgcgt gcgggaaacg ctgccacgcc ggcgggtgca acaccgccac ctcgcgccgc
     7201 accgcttcca gcgtttccat gactcgcgcg ccacggtcag gtcggtattc gaggtgcaac
     7261 aggaaatcga acagcgcgaa ttccagttgc cgcaccagga acatgccggc ctggaaatgc
     7321 cgcgcggcca gcatgttgtc gaacagttcg ccgggaagcg gctcgcccgt cgaccagtgc
     7381 ctcgcgaaca ggtcgagcgc ccgccggttc cagcagaagt tctccatgaa ctggctcggc
     7441 atttcgaccg cgtcccattc gacgccatct atcccgccga tcgagggcag ttcgatgcgg
     7501 gtcagcaggt ggtgcagtcc gtgcccgaac tcgtggaaca tcgtgagcac gtcgtcgtgg
     7561 gttagcagcg acggatgtcc acccgcgggt ggagcgaaat tgcaggtgag gaacgcgatc
     7621 ggcaactgcg tgcggtcgcc gtcgcggaag cgcgcacggc acacgtccat ccatgccccg
     7681 ccgcgcttgc cgtcgcgcgc atggaggtcc aggtacacac ccgcgatcag tgcgccgtcg
     7741 gcatcgcgca cttcgcagta acgcacgtcc ggatgccaca cgtccacggg ctcgcgcgcg
     7801 gattcgacgc tgatgccgta caacttgccg gccacggtga acaggccgtc caacaccgac
     7861 ggcaggggga aatacgcctt caactgctcc tcgtccagcg cgtagcgcgc acgccgcagt
     7921 ttctcggacg cgtaggcgac atcccagggt tcgagggtct ccagcccgag ttcgctgcgc
     7981 gcgaattcgc gcagttcatc aagctcgcgc tgggccaccg gtttcgcgcg cgccgccagg
     8041 tcgcgcagga acgtgagcac ttcgtccggg gtcgtggcca tcttggtggc cagcgattcc
     8101 tcggccgcgt tcgcgaagcc gagcaattgc gcggcttcgt ggcgcagcgc caggatcttt
     8161 tccatccgcg ccgagttgtc gaacttgccg gcgttcggcc cctggtcgga tgcgcgcgtc
     8221 tggtacgccc aatacacacg ctcgcgcaag ccgcggttgt cggcgtaggt caacaccgcc
     8281 tgcaccgccg gctgcttcag ggtcaccagc gatccttcga gctcgcgctc acgcgcatat
     8341 tcgcgcagca ccgcgcggcc cgattccggg atgcccgaga ggtcgcgctc gtcctcgatc
     8401 cgttcgtacc aggcttcgct ggcgtccagc accgcattgg cgaagccggt ggtgagcttg
     8461 gcgagttcgt tgacgatttc ccggaagcgc gtgcgcgcag gctcctccag cgcgacgccg
     8521 gacagcctga agtcgcgcag cgcgtgctcc accagcgcgc gttccggccg cggccgcgcc
     8581 gcgaagccgg gggcatcggc gaccgcctgc acggccgcgt aaagatcacg gttctggccg
     8641 agggcgcttt cgaaatcggt gatcttctcg agtgccgcgt cgtaggccgc gcgcaattcc
     8701 ctggtgtcgg cgaccgcgtg caggtgcgaa accggcgccc aggcacgtgc aagccgctgg
     8761 tcgaggcgct cggattccag catcacgtgt tcgaagtccc gcggcgacgc ggccgccgtc
     8821 agcaaatcga tgccagcctg gtagtgcgcc aggatggcgt cgatggccgg ctcgacatcg
     8881 gcggcgcgga tcttcgagaa cgcgggcagt tgcgcgtcgc cgagcagggg attgtcgttc
     8941 gacatggtcg ctccggggat tctccagcgt ggcgttgcct ggtccggaaa tcaagctagc
     9001 atcgcggcat gcgaacgaca ccggccgtgg atctgcgcgc attgctcgcc gcgaccgcgg
     9061 tgttcgggca cctcgaccgg ggcgagctgg gcgcgttgct cgaggaaatg gcttggttcc
     9121 tgttgccggg cggcgcgacg ctgtaccagc gcggcgagga acccgacgcc ctgttcctgc
     9181 tgaagtccgg cagcctcggc ctgttcggca cgccgctgat cggcggcccg gcgcgcctgc
     9241 tcggcgtgat tccggccggg gaaacgctgg gcgaaatcgg cctgctcgtc ggcgaggaac
     9301 gcacccacag cgtgcgcgcg ctgcgcgatt cggaattgct gcgcctgtcg cgggaaggtt
     9361 tcgagaagct cgtggcgcgg cacccgcagg cgatgttgag cgccgcgcgc gtcgcgatcc
     9421 agcgcctgct cggcgatccc cacgaggagg cgaccagccc gccgcgcacc ttcgcggtgc
     9481 tgccgttcga ccacgaaatc gacgcgcacg ccttcgcgga aagcctgcaa caccagctgc
     9541 tcgcatgggg cgattgcctg gtgatcgacg ccgggctcgg ccggtcgcgc gacaccggct
     9601 ggttcaccga acgcgaatcc gaacaccggt tcgtgctgta cctcggcgac ggcgacccga
     9661 cctggcgccg gttgtgccag cgccaggccg acgcgctgct gctgttggtg gcggccgagc
     9721 gtacgcccga gccatggccg tacccggtcg gcaaccggcc ggcggccgac gccggccacc
     9781 cgcaaagcca cgaagccatg gtcgaagaca cccatcaccg gccgcgccac ctggtactgc
     9841 aacaccctcg cgggcagttc atctacgggg ccgcccgcgc ctggcgggcc agcatcggca
     9901 cggccgaact gcaacaccac cattggcgcc accacggcga catggcccgg atcgcgcggc
     9961 tgttgtccgg caacagcacg gctctcgtgc tgtcgggcgg cggcgcccgc ggattcgcgc
    10021 agatcggcgt ggcgcaggca ctgcgcgaag ccggcatcag cgtcgacacg gtggtcggca
    10081 ccagcatcgg cgcgatcatc ggtgccggca tcgcgtacga atgggacgac ggcaagttgc
    10141 gcgcggcctg ccgccgcgcg ctggtggacg gcaagccgct cgccgactgg acgctgccgc
    10201 tggtcgcgct gacccgcggc gcgcgcgcca cgatgctgat gcgggaggcc ttcggcgaga
    10261 tcgacgtcga ggacctgccg gtgccgttcg cctgcgtctc ggcggatctc acggatggcg
    10321 ccaccgcggt ccaccgcgag gggctcctgt ggcgctggat cagggcatcc agcgcggtgc
    10381 cgggaatcct tccacccttg ttccacgacc accacgtgta cgtcgacggc gcggtgatca
    10441 acaacctgcc gaccgacgtg ctggccgagg acgcaccggg gcggatgatc gccgtggaca
    10501 tcggggcgga cgacaccttg cgggcggagg tcaacgaggc agcctcgccc cgctggccga
    10561 ccctgtgggc ggagcgccgc acccgccagc ggcgccccgg cgccttcgcc atcctgatcc
    10621 gcgccggcat ggtcaattcc gagaccggca gcgcgcgaag gcgcgagctg gccgacctgg
    10681 tgctgcgccc cgacatgcag gaaatcggga tgttcgactg gcaccatttc gaccgcgcca
    10741 tcgagatcgg ctatcgcagc acccgtgatg cgctgcgcga acacactgct ccgaccggaa
    10801 aatcgtgaac cgacgcgggc gcggccggtg caggacgcac ccgggcgcgg gcgtaccgcc
    10861 ggatccatcc cttcgttacg atggcgcgtc atgatccgcc aggaacaatt gacatggcca
    10921 acttgcgcgc ttatcggggt gtcgagccac ggctcggcgc acgcgtgtat gtcgaccccg
    10981 ccgccaccgt gatcggcgat gtggaactcg gcgacgacgt gtcgctttgg cccggcgcgg
    11041 tggtgcgtgg tgacgtcgag aaaatccgca tcggcgcgcg cagcaacgtg caggaca
//
LOCUS       DASSNH010000273         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1268081, whole genome shotgun sequence.
ACCESSION   DASSNH010000273 DASSNH010000000
VERSION     DASSNH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268081"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFK00_10025"
     CDS             complement(<1..762)
                     /locus_tag="VFK00_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7593329.1"
                     /translation="MTEDAAERVRPIDLLITARWIVPVEPDRVVLEGHAVAIDHGAII
                     EVLPRAEADAKYAPGDRVDLPQHALIPGLVNAHTHNPMTLLRGLADDLPLMTWLQEHI
                     WPVEAKVIGPAFVRDGVELAIAEMLRGGTTCCNENYFFPDAQAAAYRAHGFRARVGLP
                     IVEFPSAWAQTRDEYFERALAVHDDLRGDPLVGTAFGPHAPYTVADASFERIRMLSDQ
                     LDVPVHLHLMETAQEVEDSRREHGLRPMQRLQKLGL"
     gene            complement(957..1523)
                     /gene="efp"
                     /locus_tag="VFK00_10030"
     CDS             complement(957..1523)
                     /gene="efp"
                     /locus_tag="VFK00_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7593330.1"
                     /translation="MASYGLNDVKNGLKIIVDGDPYVIVGADFIKPGKGQAFTRIKIR
                     NLKNGRTTEKTMKATDTVEGADVVDTDMQYLYNDGEFWHFMDPASYEQHTAEKNAVGD
                     AAQWLKGEEECIVTFWNGVPLSVLPPNFVELKIVETDPGLRGDTSGGGGKPAKLETGA
                     TVRVPLFVNQDEVIKVDTRTGEYVSRVK"
     gene            1760..2770
                     /gene="epmB"
                     /locus_tag="VFK00_10035"
     CDS             1760..2770
                     /gene="epmB"
                     /locus_tag="VFK00_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HET7593331.1"
                     /translation="MITAIAQATQPDALPDWRQSLREAVTDPRELLALLGLPHLAGRL
                     PADDAGFRLKVPHAFVARMRHGDPDDPLLRQVLPQLAECDEAPGFGDDAVGDLAAIAG
                     HGVLHKYEGRALLIAAGSCAVNCRYCFRRHFPYAEELAAAHAWQEAVTCLAADSSIRE
                     VILSGGDPLVLSTQKLTNLTDALRDLPHVIRLRIHSRLPVVLPERIDAELCAWLATLP
                     LQRVMVIHANHPNELDASVGAACARLRDAGATILNQSVLLRGVNDDAGVLAGLSERLF
                     ALGVLPYYLHQLDRVRGTAHFEVTDARARDLVDALRDRLPGYLVPRLVREVPGELAKR
                     PL"
     gene            2959..>3204
                     /locus_tag="VFK00_10040"
     CDS             2959..>3204
                     /locus_tag="VFK00_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7593332.1"
                     /translation="MLPEPITKLIIAEDQAEDAEQTISVLRNSGIALRPQRISTEAEL
                     AACLESFTPDLVLANPGCKEISIETIAHALDGTGKDVA"
BASE COUNT          508 a         1073 c         1041 g          582 t
ORIGIN      
        1 gagccccagt ttctgcaagc gctgcatcgg ccgaaggccg tgctcgcgcc gggagtcctc
       61 gacttcctgc gccgtttcca tcaagtgcag gtgcaccggc acgtccaact ggtccgacag
      121 catgcggatg cgttcgaatg atgcgtcggc gacggtgtag ggcgcgtgcg gcccgaacgc
      181 ggtgccgacc agcggatcgc cgcgcaggtc gtcatgcacc gcgagcgcac gctcgaaata
      241 ttcgtcgcgg gtctgggccc acgcgctcgg aaactcgacg atcggcaacc cgacgcgcgc
      301 gcggaagccg tgggcccggt aagccgcggc ctgggcatcg gggaagaagt agttttcgtt
      361 gcagcaggtg gtgccgccgc gcaacatttc ggcgatcgcg agttcgacgc cgtcgcgcac
      421 gaacgcggga ccgatcacct tggcttcgac cggccagatg tgctcctgga gccacgtcat
      481 caacggcagg tcgtccgcga gcccgcgcag cagcgtcatc gggttgtggg tgtgcgcatt
      541 gacgaggccc gggatcagcg catgctgcgg caggtcgaca cgatcgcccg gggcgtattt
      601 cgcgtcggct tcggctcgcg gcaagacctc gatgatcgcg ccgtggtcga tggcgaccgc
      661 atgcccttcc agcaccacac ggtcgggctc gaccgggacg atccagcggg cggtgatcag
      721 taggtcgatg ggacgaacgc gctcggctgc atcctcggtc atgacgatct gctcttgctc
      781 gtcattccgg cgcaggccgg aatccattca tggattgtgg atggcccgga cttccaccgg
      841 gttcgtaatg taatccgctg cgcggatttg ctgcgaacat ccgaacatgg accccggctt
      901 tcgccggggt gattgattcg cgacgcaatc cgctgcgcgg atttgctgcg aatcaatcac
      961 ttcacccggc tgacgtactc gccggtgcgg gtatcgactt tgatgacctc gtcctggttg
     1021 acgaacagcg gcactcgaac agtcgcgccg gtctcgagct tggccggctt gccgccgccg
     1081 cccgaggtgt cgccgcgcag gccgggatcg gtttcgacga tcttgagttc gacgaagttc
     1141 ggcggcagca ccgacagcgg cacgccgttc cagaacgtga cgatgcactc ctcctcgccc
     1201 ttcagccatt gcgccgcgtc accgaccgcg ttcttctcgg cggtgtgctg ctcgtagctc
     1261 gctggatcca tgaagtgcca gaactcgccg tcgttgtaga ggtactgcat gtcggtgtcg
     1321 acgacgtccg cgccttcgac cgtgtcggtc gccttcatcg tcttttcggt ggttcgcccg
     1381 ttcttgaggt tgcggatctt gatgcgcgtg aacgcctggc ccttgccggg cttgatgaaa
     1441 tccgcgccga cgatcacgta cggatcgccg tcgacgatga tcttcagacc gttcttgacg
     1501 tcgttgagac cgtagctggc catgctgctt gctccgtgat ttatgaggtc gcaaaacggg
     1561 gcatcctgcc acggtttgca agcaaacgga cgcggcaaag ccgcgccacg tttgccgcgc
     1621 cagacacaat cgtgccttcc gcgtcggcac ttccgtgtgc cgaagtcatc cgtcgaccgt
     1681 cgggctcgaa cacctgattc ggcctcgcga ttgacctaaa atggtgggtt tccggcgacg
     1741 caccggaaac ggacccgcca tgataaccgc aatcgcccaa gccacgcagc cggacgccct
     1801 gccggactgg cgccaatcgc tgcgcgaggc ggtgaccgat ccgcgcgagc tgctggcgct
     1861 gttgggcctg ccgcacctcg ccggacgcct gcccgccgac gatgccggtt tccggttgaa
     1921 ggtgccgcac gcgttcgttg cgcggatgcg ccacggcgat ccggacgatc cgctgttgcg
     1981 ccaggtgttg ccgcaactcg ccgagtgcga cgaggcgccc ggcttcggcg acgacgccgt
     2041 cggcgacctc gccgccatcg ccgggcacgg cgtgctgcac aagtacgagg gccgtgcgct
     2101 gctgatcgcc gccggcagtt gtgccgtcaa ttgccgttac tgcttccgcc ggcattttcc
     2161 ctacgccgag gaactcgcgg ccgcgcacgc atggcaggaa gccgtcacct gcctcgcggc
     2221 cgacagctcg atccgcgagg tgatcctgtc cggcggcgac ccgctggtgc tatcgacaca
     2281 aaagttgaca aacctcaccg acgccctgcg cgacctgccc cacgtcatcc ggctgcgcat
     2341 ccattcgcgg ctgccggtgg tgttgcccga gcgcatcgac gccgaactgt gcgcctggct
     2401 ggcgaccctc cccctgcagc gcgtcatggt gatccatgcg aaccacccga acgagctgga
     2461 cgcgagcgtt ggcgcggcct gcgcgcgttt acgggatgcc ggcgcgacga tactcaacca
     2521 gtccgtgctg ctgcgcggcg tcaacgacga cgccggcgtg cttgccggac tttcggagcg
     2581 attgttcgcc cttggcgtgc tgccctacta cctccaccag ctcgaccggg tccgtggcac
     2641 cgcgcatttc gaggtcaccg atgcccgcgc gagggacctg gtggacgcgt tgcgcgaccg
     2701 ccttcccggt tatcttgtgc cgcggctggt gcgcgaagtt ccgggggaac tcgccaagcg
     2761 cccgctctga tccttcgcgg tggcgccggc cttcccgata gggtaaaaag tcctgtcggc
     2821 cggccgatat aggctgcagg gtgcgtcgcg gccggggtcg gcttcgagcg gccgatggac
     2881 gcaaaacgag ggatccggcc gcgactgcct ggtgccgaac ccagccgcct gtccagaagt
     2941 ttttgccaaa ggtcgtccat gttgccggaa cccatcacca agctgatcat cgccgaagac
     3001 caggccgagg acgcggagca aaccatcagc gtgctgcgca acagcggaat cgcgctgcgt
     3061 ccgcagcgca tttcgacgga agcggaactg gcggcgtgcc tggaatcgtt cacgccggac
     3121 ctggtgctgg cgaatcccgg ctgcaaggaa atctcgatcg aaaccatcgc ccacgcgctc
     3181 gatggcaccg gcaaggacgt cgcg
//
LOCUS       DASSNH010000274         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_773759, whole genome shotgun sequence.
ACCESSION   DASSNH010000274 DASSNH010000000
VERSION     DASSNH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773759"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFK00_10045"
     CDS             complement(<1..663)
                     /locus_tag="VFK00_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7593333.1"
                     /translation="MADGNPTLDQQCINTIRFLSVDMVQKADSGHPGMPLGAAPMGYV
                     LWTKFLKFNPANPSWADRDRFVLSAGHGSALLYSLLYLTGYDVSLDDIKQFRQWGSKC
                     PGHPERGHTPGVEVTTGPLGQGFANAVGMAMAEAQLAARYNREGHKVVDHYTYAIASD
                     GDLMEGVASEAASLAGHLKLGKLIVLYDDNYVTLSAGTDITFTEDRAARFKAYGWHVQ
                     TVN"
     gene            803..928
                     /gene="ykgO"
                     /locus_tag="VFK00_10050"
     CDS             803..928
                     /gene="ykgO"
                     /locus_tag="VFK00_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HET7593334.1"
                     /translation="MKVLSSLKSAKQRHRDCKVVRRRGKVFVICKSNPRFKARQR"
     gene            1136..1414
                     /locus_tag="VFK00_10055"
     CDS             1136..1414
                     /locus_tag="VFK00_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593335.1"
                     /translation="MVAATPFALPAHAESIRTKVAQEQTMNLPQRGMTMAQVERKFGA
                     PARKLDTRGGDSPKHPPIHRWEYSNYIVYFERNHVIHSVITTPDTGAH"
     gene            1419..>2122
                     /locus_tag="VFK00_10060"
     CDS             1419..>2122
                     /locus_tag="VFK00_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET7593336.1"
                     /translation="MTAADFRLPAEWEPQAAVLLAWPHSDTDWGPRLLAAESSYVPLV
                     TAITRFERALVCVPDFAVRERAAALLDSVGADLSRIGFIEIPYDDTWLRDSGPITLRC
                     GGEFTLADFRFTGWGGKFEGSRDDALVAGLLQRDLFRAGTGRARYDWALEGGAIESDG
                     RGTILTTWRCLHQRHPEQSREEMSRQLRESLGARRVLWLERGYLQGDDTDAHIDTLAR
                     FAPNDAIVFQACDDPA"
BASE COUNT          351 a          716 c          682 g          373 t
ORIGIN      
        1 attgacggtc tgcacgtgcc agccgtaggc cttgaaacgc gccgcgcgat cctcggtgaa
       61 cgtgatatcg gtgcccgcgg agagcgtcac gtaattgtcg tcgtacagca cgatcagctt
      121 gccgagcttc aggtgccccg caagcgatgc ggcttccgag gccacgcctt ccatcaggtc
      181 gccgtcgctg gcgatcgcgt aggtgtagtg gtcaacgacc ttgtggccct cgcggttgta
      241 gcgcgcggcg agctgcgcct cggccatcgc catgccgacc gcgttggcga agccctgccc
      301 cagcggcccg gtggtgactt ccacgcccgg ggtgtggccg cgctcgggat ggcccgggca
      361 cttgctgccc cactggcgga actgcttgat gtcgtccagc gaaacgtcat agccggtgag
      421 atacagcaag ctgtagagca gcgccgaacc gtgccctgca gagagcacga agcggtcgcg
      481 gtccgcccag ctcgggttgg ccggattgaa cttcaggaat ttcgtccaca gcacgtaacc
      541 catcggcgcg gcgcccagcg gcatgccggg gtggccgctg tcggccttct gcaccatgtc
      601 caccgacagg aaccggatcg tgttgatgca ttgctgatcg agagtggggt tgccgtctgc
      661 cacgatcctt ctccgattcg cgccatggaa accgccaagc atacgcccgg gccgtggacg
      721 cctcgtcagg cttgccacaa gcctttgccc cggctacact ttccggcttg cttcaacccg
      781 aatacgcccc gaggagccta ccgtgaaagt gctttcgtcc ctgaagtccg cgaaacagcg
      841 gcatcgcgac tgcaaggtcg tgcgccgtcg cggcaaggtg ttcgtgatct gcaagtcgaa
      901 cccgcgtttc aaagcccgtc agcgctgaat ctcctgcgag ctgctgcgct cgcggaagat
      961 tcggatgatg acccgaaagg ccgccccgcg cggccttttt tgttgctgaa cgaatgtcgg
     1021 gcggatcatg gaatgccgcg ccgccttctg ttaaaacaac accggtcggc gccagatgcg
     1081 ccgggggagt catgccatga aaagcattag ccgttccctg ttcgttgccg ccttgatggt
     1141 cgccgccacc ccgttcgccc tgcccgccca cgcggaaagc atccgcacga aggtggcgca
     1201 ggagcagacc atgaatctgc cgcagcgagg catgaccatg gcgcaagtgg agcgcaagtt
     1261 cggcgcgccg gcccgcaagc tggacacccg cggcggcgat tcacccaaac acccgccgat
     1321 ccaccgctgg gaatacagca actacatcgt gtatttcgag cgcaatcacg tgatccatag
     1381 tgtgatcacg acgcctgaca ccggcgcgca ctgatcgaat gaccgcagct gattttcgac
     1441 tgcctgcgga gtgggagccg caggcggcgg tgctgctcgc gtggccgcat tccgacactg
     1501 actggggtcc gcggctgctc gccgccgaat ccagctacgt ccccctggtg accgcaatca
     1561 cgcgcttcga gcgcgcgttg gtatgcgtgc ccgactttgc cgtgcgggag cgcgcggcgg
     1621 ccctgctgga ttcggtgggt gcggatctct cgcgcatcgg cttcatcgag atcccctacg
     1681 acgacacttg gctgcgcgat tccggcccga tcacgcttcg ctgcggcggc gaattcacgc
     1741 tggccgattt ccggttcacc ggctggggcg gcaagttcga gggctcgcgc gacgacgcgc
     1801 tggtcgcagg cctgctgcaa cgcgatctct tccgcgccgg caccggccgc gcgcgctacg
     1861 actgggcgct ggaaggcggc gcgatcgaat ccgatggccg cggcacgatc ctcaccacct
     1921 ggcgctgcct gcaccagcgc caccccgagc agtcgcgcga ggaaatgtcg cggcagttgc
     1981 gcgaatcgct cggcgcgcgg cgcgtgctgt ggctggagcg cggctacctg cagggcgacg
     2041 ataccgacgc gcacattgac acgctcgcgc gcttcgcgcc gaacgatgcc atcgtgttcc
     2101 aggcctgcga cgatccggcc ga
//
LOCUS       DASSNH010000275        11744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1782857, whole genome shotgun sequence.
ACCESSION   DASSNH010000275 DASSNH010000000
VERSION     DASSNH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782857"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFK00_10065"
     CDS             complement(<1..276)
                     /locus_tag="VFK00_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HET7593337.1"
                     /translation="MFDALRHPLLARIDSPADLRRLLEQELPALADELRAYLIESVAS
                     VGGHFGAGLGVVELTIALHYLYDTPHDRLVWDVGHQGYPHKILTGRRD"
     gene            complement(477..2324)
                     /locus_tag="VFK00_10070"
     CDS             complement(477..2324)
                     /locus_tag="VFK00_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7593338.1"
                     /translation="MPRESTLVSRRILLISALAIVLGAAAAGAAQLLMLMINVITDIT
                     FYGRVSDVFGPNALPRSAVLSPADNHLGAWVIAMPVIGGLIAGVMARWGSRAIQGHGI
                     PEAMERILEHESNIPPRVTWLKPVSSAFAIGTGGPFGAEGPIISTGGAMGSLIGQFLS
                     VTAQERKVLLAAGAAAGMAAVFGAPVASLVMAVELLLFELRPRSLIPVALATVTATGV
                     RYATFGSAAVFAMPSVTEPGGWALASYIVLGAIVGYASVWVTRAVYGVEDLFEKLPIH
                     WMWWPAIGAVVAGVIGWISPHTLGVGYDNIDALVQGNFGVTMLLTFGALKFLSWVIAL
                     GSGTSGGTLAPLFMIGGSLGALVGIGIGDVWPWFGIDSRIAALVGMAAIFAGSSRALL
                     TAIVFAFETTRQPEALLPLLAGCAAAYLISALSMRSTIMTEKIERRGIRVPSEYAADF
                     LEQVKVGKVCTREVRALKTSDELGEVRKWLNSGRVRAKHQGYPVVDDAGRIRGVVTRR
                     DLLDPDRAGLTRIGDMLTRGPIVVFEDHTLRDAADLMVTENVGRVIVVPREDPTRMVG
                     FLTRSDVMAANSRRIHEMRDAKREIRIRQSFARRFGGRARPAADESKAS"
     gene            complement(2559..3179)
                     /locus_tag="VFK00_10075"
     CDS             complement(2559..3179)
                     /locus_tag="VFK00_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7593339.1"
                     /translation="MGPHTATVIEHPAYAAADLHTTRVLSLDAAGRILDWISWQDAAC
                     LYVRDAVAWTLGDPCLTIHGGFRRDTGDQSILELHPIVASRGHCHSGAIDPSPALSNA
                     ALFARDRYLCLYCGRHCGRSELTRDHVVPLSRGGRDVWENVVSACLACNLKKGGHTPQ
                     QAAMPLLAVPYKPSWVEHLILSNRNILADQMEFLMHHLPRNRRPAA"
     gene            3426..5246
                     /locus_tag="VFK00_10080"
     CDS             3426..5246
                     /locus_tag="VFK00_10080"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET7593340.1"
                     /translation="MADVFVSYARADKARVAPLVAAIEAKGWSVWWDPEISPGQEFDD
                     QIEAEIDAAKAVLVVWTPTSVASRWVRGEAREAAERNILVPVRFGQARLPMDVRAIHT
                     TDLDDWGEDARHSAMQECLRALAAMIASSSAAQSAKEAVETAQSRDEKKVARYSICVL
                     PFANMSGQPEQEYFSDGITEDIITDLSKVSSLRIISRNSAFMYKGQNVDVPKVARELN
                     VTHVLEGSVRKAGDRVRISAQLVDVEDNGHLWAERYDRDADDIFAIQDEISKAIVKAL
                     RLRLLPEEKKAIERRGTDSADAHNLYLMARQAYVMGQEGDSHSAQVIVRICGRATEID
                     PRYAEAWALMALGYRQWSEAGWSEAGRGGASTGDAMAAVERALALNPDLAEAHAVKAQ
                     ILQLEDDLEGSRAEVATALSLDPESYEVNRSAARLHYQLRAFDRAIHCYEKTVSLMDA
                     DVNSAAMLVSCYTAINDKEGIRRSAELALKRAEAVLARDPNSSVATAYSVDALAALGE
                     GERAKARMERALLIDPDNWNMRYNFACMLSVQLKDKDAALAMLEPLFTTVASSFLRYA
                     KADPDLELLCDDPRYQAMIAAAEARLMAEKSAVPPAKVEA"
     gene            5340..7139
                     /locus_tag="VFK00_10085"
     CDS             5340..7139
                     /locus_tag="VFK00_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET7593341.1"
                     /translation="MADVFISYARADKARVAPLVAAIEARGWTVWWDPEIAPGRQFDD
                     EIDTALQAAKAVLVVWTPTSVVSRWVRGEARDAAERGILVPVRFDQARLPIDVRAIHT
                     TDLDDWHGDPAHPAVRECLQALATMIASSETAQAADGLSTTAESGASEKPRPKFSICV
                     LPFVNMSGEPEQEYFSDGMTEDIITDLSKVSALAVAASNTAFRFKGQHVDIPQVTRDL
                     KVTHVLEGSVRKAGGRIRINAQLIDGASGNHVWAERYDRDANDIFAVQDEISQAIVKA
                     LRLRLLPEEKRAIEHRGTDSADAHNFYLMARQTYITGQEFDIKTAEAIVRLAKRATEI
                     DPGYAHAWALMAVGYRNVHFFEGGSDDGMAAAERALELDPGLAEAHAVKAYILLAQGD
                     TEAAAAETAAAMKLDPESYEVNRSAGRLSYQLHRLDDAIRFFEKAVALMDADINSAAM
                     LVSCYTGIGDVAGTRRAAELTLKRAEVILTRDPNNSVVISYSADALAALGEGERAKAR
                     MERALLIDPDNFNMRYNYACMLCVRLGDKDAALAALTPLVGKITGAFLEFVKADPDLE
                     LLHDDPRYQAMIAAIEARLSAAKLADKPVEVKA"
     gene            complement(7250..7534)
                     /locus_tag="VFK00_10090"
     CDS             complement(7250..7534)
                     /locus_tag="VFK00_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593342.1"
                     /translation="MWIARTSIGRRAFSNRTGTRIPRSAASRASPRTQRETTEVGVHT
                     TSTALAASISASIWSSNSCPGVISGSHHTVQPSASIAATSGATRALSARA"
     gene            7512..9026
                     /locus_tag="VFK00_10095"
     CDS             7512..9026
                     /locus_tag="VFK00_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593343.1"
                     /translation="MDVRAIHTTDLDGWHDDAAHPAVQECLRALEAMIARSQATQAGK
                     PTDAPAASSDKKPSPRYTVCVLPFANMSGDPDQEYFSDGITEDIITDLSKVSSLRIIS
                     RNSAFQYKGEKLDVPQLVREMKVTHVLEGSVRKAGGRVRISAQLVDCEDNGHIWAERY
                     DRDTSDIFALQDEISRAIAKALRLRLLPEEKKAIERRGTDSAEAYDLYLMARQMYVTG
                     QEYDEHAAQAIIRMCTGATDIDPRYAQAWALMAIGYRSLRGLGVGAESGMDAAERALA
                     IDPGLAEAHAVKAYVLLQNGDTDAAESEAAVALNLDPESYEANRTAARVNYQLHRFED
                     AIRLYEKAAHLMEADINSVMTLVSCYTVTGNAAGVRRSAELGLNRAEAILARDQNNPG
                     VLAYSAYALMALGEGDRAKARMNRTLMIDPSNWNMRYNFACSLCVYLKDKEAALEILG
                     PLFETISDSFFPYAKADPDLASLRDEPRYQAMVAVAEARLAASKTAAPEMVAGS"
     gene            9032..10828
                     /locus_tag="VFK00_10100"
     CDS             9032..10828
                     /locus_tag="VFK00_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET7593344.1"
                     /translation="MADVFVSYARADKARVAPLVAAIEAKGWSVWWDPEISAGQEFDD
                     QIEAEIDAARAVLVVWTPTSVASRWVRGEAREAAERGILVPVRFEQARLPMDVRAIHT
                     TDLDAWGEDSTSPLMQECLHALGAMIARSQIAQAAKDASATSVTPKSSKNAARFSICV
                     LPFVNMSGDPEQEYFSDGITEDIITDLSKVSALEVVSRNGSFLYKGKQVDVPKVAHEL
                     KVTHVLEGSVRRAGGRVRISAQLIDGARNRHAWAERYDRDASDIFALQDEISHAIVKA
                     LRLQLLPEEKKAIERRGTDSVEAHDLYLMARQLYVTSQDDEHASRAIVRACARATQID
                     PNYAQAWALKAVGALNLCDLGLETDDGMAAAERALELDPDLVEAHAVKGYILRMRGNM
                     EGAITEASIAMKLDPESYEANRAAARISYVQHRFEDAVRLYEKAVSLMDSDVNSAMLL
                     VSSYRALGDVVGMRRAAEVALKRADAVLARDQSNSYVLAYSANALGALGEGERAKARM
                     SRAMLIDPDNWNMRYNFACTLSMYLQDTDAALEILESLLDTATGSLLDYIKDDPDLDS
                     LHDNPRYVAMVAATEARLAAAKQDEAPALQKA"
     gene            10935..>11744
                     /locus_tag="VFK00_10105"
     CDS             10935..>11744
                     /locus_tag="VFK00_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET7593345.1"
                     /translation="MADVFISYARADKARVAPLVAAIEAKGWTVWWDPEIAPGRQFDD
                     EIDVALQAAKAVLVVWTPTSVVSRWVRGEARDAAERGILVPVRFDQARLPIDVRAIHT
                     TDLDGWHEDPAHPAVQECLHALDAMIARAPADAGEAARGSAPAPGKPAPRFSICVLPF
                     VNMSGDPEQEYFSDGITEDILTDLSKISALSIISRNGSFLYKGKQVDIPQIARDLNVT
                     HVLEGSVRKAGGRVRISAQLIDGTANNHVWAERYDRDASDIFALQDEISHAI"
BASE COUNT         2157 a         3638 c         4004 g         1945 t
ORIGIN      
        1 atcgcggcgg ccggtgagga tcttgtgcgg atatccctgg tggccgacgt cccacacgag
       61 gcggtcgtgc ggggtgtcgt acaggtaatg cagcgcgatg gtgagttcga ccacgccgag
      121 cccggcgccg aagtgcccgc ccacgcttgc gaccgattcg atcaggtagg cgcgcaattc
      181 gtcggcgagc gcgggcaact cctgttccag cagccgtctc aggtcggccg gtgaatcgat
      241 ccgggcaagc aggggatggc ggagggcgtc gaacatcgcg ccattgtcgc gcagggcgtg
      301 ccgaaatgct gggctgcttt gcaaccgtcc gtcgtacgcg aacatccccc cgatgctgcc
      361 tgctgcagca tcgcccccct tcctgcggaa gggggcattc gagctggtct tcccccttcc
      421 gcaggaaggg ggatcgaggg ggatggcttt tttgcccgag cgaaaaccga acggtcttac
      481 gacgccttgg attcgtcggc agcgggtcgc gctcgccctc cgaagcggcg cgcaaagctc
      541 tgccggatcc ggatttcgcg cttggcgtcg cgcatttcat ggatgcgtcg gctgttggcg
      601 gccatcacgt cgctgcgggt caggaaaccg accatgcggg tcgggtcttc gcgcgggacc
      661 acgatcaccc ggcccacgtt ttcggtcacc atcaggtcgg ccgcgtcgcg cagggtgtgg
      721 tcctcgaaca ccacgatcgg tccgcgggtc agcatgtcgc cgatccgggt caggcccgcg
      781 cggtcggggt cgagcaggtc gcgccgcgtg accacgccgc ggatccgccc cgcgtcgtcg
      841 accaccggat agccctggtg cttggcgcgc acgcggccgg agttgagcca cttgcggacc
      901 tcgccgagtt cgtcggaggt cttgagggcg cgcacctcgc gcgtgcagac cttgcccacc
      961 ttcacctgct ccaggaaatc cgcggcgtat tcgctcggca cacggatgcc gcgccgctcg
     1021 atcttctcgg tcatgatcgt gctgcgcatg ctcagtgcgg agatcaggta cgcggcggcg
     1081 cagcccgcca gtaacggcaa cagggcttcc ggctggcgcg tggtttcgaa cgcgaacacg
     1141 atcgccgtca ggagcgcgcg ggaagaaccg gcgaagatcg ccgccattcc gaccagcgca
     1201 gcgatccgcg agtcgatgcc gaaccacggc cagacgtcgc cgatcccgat gccgaccagc
     1261 gcgcccagcg aaccgccgat catgaacaac ggcgccagcg tgccgcccga ggtgccgctg
     1321 cccagcgcga tcacccacga caggaatttc agcgcgccga aggtgagcag catcgtgacg
     1381 ccgaagttgc cctgcaccaa tgcgtcgatg ttgtcatagc cgacaccgag tgtatgcggc
     1441 gagatccagc cgatgacgcc cgccacgaca gcgccgatcg ccggccacca catccagtgg
     1501 atcggcagtt tctcgaacag gtcctcgacg ccgtacacgg cacgggtgac ccaaacgctc
     1561 gcatagccga cgatggcgcc gagcacgatg tagctggcca gcgcccagcc gccgggctcg
     1621 gtcaccgacg gcatcgcaaa taccgctgcg gaaccaaaag tcgcgtagcg cacgccggtc
     1681 gcggtgacgg tcgccagcgc gaccgggatc agcgaccgcg gccgcaattc gaacagcagc
     1741 aattcgaccg ccatcacgag cgatgcgacc ggcgcaccga acaccgccgc catgccggcc
     1801 gcggcgccgg cggccagcaa caccttgcgc tcctgcgcgg tgacgctcag gaactggccg
     1861 atcagcgagc ccatcgcgcc gccagtcgag atgatcggcc cctcggcgcc gaacgggcct
     1921 ccggtgccga tcgcaaacgc cgacgacacc ggcttcagcc aggtcacccg cggagggatg
     1981 ttcgactcgt gttcgaggat ccgctccatg gcctcgggga tgccatggcc ctggatcgca
     2041 cgcgagcccc agcgcgccat cacgcctgcg atcagcccgc cgatcaccgg catcgcgatc
     2101 acccaggcgc cgaggtggtt gtcggcgggc gaaagcaccg ccgagcgcgg cagcgcgttg
     2161 gggccgaaca cgtccgagac ccgcccgtag aacgtgatgt cggtgatcac gttgatcatc
     2221 agcatcagca gttgcgcggc gcccgcggcc gcggcgccga gcacgatcgc cagcgcgctg
     2281 atcagcagga tccgcctgga taccagcgtc gattcgcgcg gcatgctcgc cgcgtcgagc
     2341 gccgcgccca gtgccggcgc gagcggcagg gcgtcggtgc tgccggtggt ccgcacgctt
     2401 tcgtgtgcgg gaccggcatg cggttcaccg gcacgagcgg acggctggtc cgccgccccg
     2461 ggggtcgggt cggttggtgt catggaaggc gaaggcaacg gctgctgcgc tgcagcataa
     2521 cgggggcgtc cggctcggcc aagggcgccg catttccgtc acgccgccgg gcgacgattc
     2581 ctgggcaggt ggtgcatcag gaattccatc tggtccgcga ggatgttgcg gttggagagg
     2641 atcaggtgct cgacccaact cggcttgtag ggcaccgcga gcaggggcat ggcggcttgc
     2701 tgcggcgtgt ggccaccctt cttaaggttg cacgcgaggc acgcggaaac gacgttctcc
     2761 cacacgtcgc ggccgccgcg cgacagcggc accacgtggt cgcgcgtcag ttcgctgcgc
     2821 ccgcaatgcc ggccgcaata caagcacagg taacgatcgc gcgcgaacag cgcggcattg
     2881 ctcaaggccg gggacggatc gatcgcgccg ctatggcagt ggccgcggct cgcgacgatc
     2941 gggtgcaact cgagaatgct ctggtcgccg gtgtcgcggc ggaagccgcc atggatcgtg
     3001 aggcacggat cacccagcgt ccacgcgacc gcatcgcgca cgtacaggca cgctgcgtcc
     3061 tgccaactga tccagtcgag gatgcggccg gccgcgtcca gagacagcac gcgcgtggtg
     3121 tgcaggtctg cagccgcgta ggcgggatgt tcgatgacgg tggctgtgtg ggggcccatg
     3181 acccgcgcag catagaacaa atgagggaat gggcgatagg catgcttccg gggaatcggc
     3241 tgcaggccgg ggattgccgg ccgcctcgaa gtgcggtccc atccgcgatc cggcgagcgc
     3301 aagcgtgccc cgccggcgca ccgcctggat tgcacggccg ccgttcgcta ccggcagcgg
     3361 cgtcactgcg gtatgcttga aagtcaatgg cttcagcgct ccacccgtca caggggttga
     3421 ccggcatggc tgatgttttt gtttcctacg cccgcgccga caaggcacgc gtcgcgccgc
     3481 tggtggcggc gatcgaggcc aagggctggt cggtgtggtg ggatccggag atcagcccgg
     3541 gccaggaatt cgacgaccag atcgaagccg agatcgacgc ggccaaggcc gtactggtgg
     3601 tgtggacgcc aacctcggtc gcctcgcgct gggtgcgggg cgaggcgcgc gaagccgccg
     3661 aacggaatat tttggtaccg gtgcgcttcg gccaggcgcg cctgccgatg gacgtgcggg
     3721 cgatccatac gaccgatctg gacgactggg gcgaagacgc aaggcactcc gccatgcagg
     3781 aatgccttcg ggcattggcg gcgatgatcg cgagttccag tgcggcgcag tccgcgaagg
     3841 aggcagtcga gaccgcccaa tcgcgcgacg agaagaaggt cgcgcgctac tcgatttgcg
     3901 tgctgccctt cgccaacatg agcggccaac cggagcagga atacttcagc gacggcatta
     3961 ccgaagacat catcaccgat ctcagcaagg tctcgtcgct caggatcatc tcgcgcaaca
     4021 gcgccttcat gtacaagggc cagaacgtcg acgtgcccaa ggtcgcgcgc gagctcaacg
     4081 ttacgcacgt gctggaaggg agcgtgcgca aggcaggtga tcgggtgcgg atcagcgccc
     4141 aactggtgga cgtcgaagac aacgggcacc tctgggcgga gcgctacgac cgcgacgccg
     4201 acgacatctt cgcaatccag gacgagatct ccaaggcgat cgtgaaggcg ttgcggttgc
     4261 ggctgttgcc cgaggaaaag aaggccatcg agcggcgcgg aacggacagt gccgatgcgc
     4321 acaacttgta cttgatggca cgacaagcat acgtcatggg ccaggagggc gactcgcatt
     4381 cggcgcaggt gatcgtgcgc atttgcgggc gggcaaccga aatcgatccc cgctacgccg
     4441 aggcgtgggc gttgatggcg ctcggctatc ggcaatggag cgaggctggc tggagcgaag
     4501 ctggccgcgg cggcgcgtcc accggtgatg cgatggccgc ggtggagcgg gcgctcgcgc
     4561 tgaacccgga cctcgcagaa gcccacgcgg tgaaggcgca aatcctccag cttgaagacg
     4621 acttggaggg ttcacgggcg gaagtggcga ccgcgctttc gcttgacccc gaatcctacg
     4681 aggtcaatcg ctccgcagcg cgcctgcatt atcagctccg cgcattcgac cgtgccatcc
     4741 actgctacga aaagaccgtg tcgttgatgg atgcggacgt caattcggca gccatgctcg
     4801 tgagttgtta caccgcgatc aatgacaagg aaggcatccg ccgcagcgcg gaattggcgc
     4861 tgaagcgagc ggaggccgtg ctggcgcgcg atccgaacag ctccgttgcc acggcctaca
     4921 gcgtggacgc gctggcggcg ctcggggaag gcgaacgcgc caaagcgcgc atggagcggg
     4981 ccctgctgat tgatcccgac aactggaaca tgcgttacaa cttcgcatgc atgttgagcg
     5041 tgcaactcaa ggacaaggat gcggcgcttg cgatgctcga gcccttgttc acgacggttg
     5101 cgtccagttt cttgcgctac gccaaggccg atcccgatct tgaattgttg tgcgacgatc
     5161 cgcgttacca ggccatgatc gccgccgccg aagcgcgcct gatggccgag aaatctgccg
     5221 ttccgcccgc caaggtggag gcgtgatgac ggcctacggt tcgtgtcgcc tcggggcggc
     5281 cacgttggct gcactgcggg aaaatcgccc ctggctgcag gattttgggg agcgtagccg
     5341 tggctgatgt cttcatttcc tacgcacgcg ccgacaaggc acgagtcgcg ccgctggtgg
     5401 ccgcgatcga ggccaggggc tggacagtgt ggtgggatcc ggagattgcg ccgggccgtc
     5461 agttcgacga cgaaatcgac accgcgttgc aagccgcgaa agccgtgctg gtggtgtgga
     5521 cgccgacttc ggtagtgtcg cgctgggtgc gcggcgaggc acgcgatgcg gccgagcgcg
     5581 ggatcctggt gccggtgcgt ttcgaccagg cgcgccttcc gatcgacgtg cgcgcgatcc
     5641 acaccaccga cctcgatgat tggcacggcg atcccgcgca tccggcggtc cgggaatgcc
     5701 tgcaggcgct cgcgaccatg atcgcgagca gcgaaaccgc gcaggctgcc gacggtttga
     5761 gcacgactgc ggaatcgggg gcttcggaaa aaccaaggcc caagttctcg atttgtgtgt
     5821 tgccgttcgt caacatgagc ggcgagccgg agcaggaata tttcagcgac ggcatgacgg
     5881 aggacatcat caccgacctg agcaaggttt cggcgctggc ggtggccgcg agcaacaccg
     5941 cgttccgatt caaggggcag catgtcgata tcccgcaagt cacacgtgac ctgaaggtca
     6001 cgcacgtcct cgaggggagc gtccgcaagg ccggggggcg catccgcatc aacgcgcagt
     6061 tgatcgacgg agccagcggc aaccatgtgt gggccgagcg ttacgatcgc gacgccaatg
     6121 acattttcgc cgtccaggac gaaatttcgc aggccatcgt caaggcgctg cgcctgcgcc
     6181 tgctgccgga ggaaaaaagg gccattgaac accgcggtac cgacagcgcg gatgcccaca
     6241 atttctacct gatggcgcgc cagacctata tcaccggaca ggaattcgac atcaagacgg
     6301 ccgaagcgat tgtcaggttg gccaagcgtg caactgaaat cgaccctggg tatgcccatg
     6361 cgtgggccct gatggccgtg ggttaccgca acgtgcattt cttcgagggc gggtccgacg
     6421 acgggatggc cgccgcggag cgagcgctgg aactggatcc gggcttggcc gaagcgcacg
     6481 ctgtcaaggc ttacatcctg ctggcacagg gggatactga agccgcggcg gccgaaactg
     6541 cggctgctat gaagctggat ccggaatcct acgaagtcaa tcgctcggcc ggacgcctta
     6601 gctaccagct gcacaggctc gacgacgcga tccgtttttt tgaaaaggcg gttgcattga
     6661 tggatgccga catcaactcg gccgccatgc tggtcagttg ttacaccggt atcggcgacg
     6721 tggcgggcac ccgcagggcg gcagagctga cgctcaagcg ggccgaagtg atcctgacgc
     6781 gcgacccgaa caattccgtc gtcatttcct acagcgcgga tgcgctggcc gcgttgggcg
     6841 agggcgagcg cgccaaggcg cgcatggagc gggccctgct gatcgacccc gacaatttca
     6901 acatgcgcta caactatgcg tgcatgctgt gcgttcgcct cggcgacaag gatgcggcgt
     6961 tggcggcact cacgccgttg gtcggcaaga tcacgggcgc attcctcgaa ttcgtgaagg
     7021 ctgatcccga tctggagttg cttcacgacg atccgcgtta ccaggcgatg atcgctgcga
     7081 tcgaagcgag gctgtcggca gcgaagctcg cggataagcc ggtagaggtg aaagcctgat
     7141 gcgccgattc gactcaggtc tggtgcgtcg tgtcgccttg cggtcggctt gcgggcaggc
     7201 atcggcaccg ccgaggggga tgaaagtccg tggctgacgt attcatttcc tacgcccgcg
     7261 ccgacaaggc acgcgtcgcg ccgctggtgg ccgcgatcga ggccgagggc tggacggtat
     7321 ggtgggatcc ggagatcacg cccgggcagg aattcgacga ccagatcgag gccgagatcg
     7381 acgcggccaa ggctgtgctg gtggtatgga cgccgacttc ggtcgtttcg cgctgggttc
     7441 gcggcgaagc gcgcgaagcc gccgagcgcg ggatcctggt gccggtgcgg ttcgaaaagg
     7501 cgcgcctgcc gatggacgtg cgcgcgatcc acaccaccga tctcgacggc tggcatgatg
     7561 acgcggcgca ccccgcggtg caggaatgct tgcgcgcgct ggaggcgatg atcgcgcgtt
     7621 cgcaagcgac gcaggccggc aaaccgacgg atgcgccggc tgcttcctcc gacaagaagc
     7681 cgtccccgcg ttacaccgtt tgcgtattgc cgttcgccaa catgagcggc gatccggatc
     7741 aggagtattt cagcgacggc attaccgagg acatcatcac cgatctcagc aaggtttcct
     7801 cgctccgcat catctcgcgc aatagcgcgt tccagtacaa gggcgagaaa ctggacgtac
     7861 cgcaactcgt gcgcgagatg aaggtcacgc acgtgctgga gggcagcgtg cgcaaggccg
     7921 gtggccgggt gcggatcagc gcccagctcg tggactgtga agacaacgga catatctggg
     7981 ccgaacgcta cgaccgcgat accagcgaca tcttcgcgct gcaggacgag atttcccgcg
     8041 ccattgccaa ggctctgcgc ttgcgcttgt tgcccgaaga gaagaaggcc atcgaaaggc
     8101 gcgggaccga cagcgccgag gcgtacgacc tgtatctgat ggcacgacaa atgtacgtga
     8161 ctggccagga atatgatgaa catgcggcgc aagccatcat ccgcatgtgc accggcgcca
     8221 ccgatatcga tccgcgttat gcgcaggcct gggctttgat ggcgatcggc taccggagcc
     8281 tgcgcggatt gggtgtggga gctgaaagcg gtatggatgc cgcggagcgc gcgctcgcca
     8341 ttgatccggg cttggccgaa gcccacgcgg tcaaggctta tgtgctgctc cagaatggcg
     8401 acaccgatgc ggcggaatcc gaagctgcgg tcgcgctgaa tctcgacccc gaatcctacg
     8461 aagccaaccg taccgcggca cgcgtgaatt accagctgca ccggtttgaa gatgccatcc
     8521 ggttgtacga gaaagcggcg cacctgatgg aggccgacat caattcggtg atgaccctcg
     8581 taagttgcta caccgtcacc ggcaacgcgg ctggcgtgcg gcgaagcgcc gagcttgggc
     8641 tgaatcgtgc cgaggccatc cttgcccgcg accagaacaa tccgggcgtc ctggcgtaca
     8701 gcgcgtatgc gctgatggcg ctgggtgagg gtgaccgcgc caaggcgcgg atgaatcgga
     8761 cactgatgat cgatccctcc aactggaaca tgcgttacaa ctttgcgtgt tcgctttgcg
     8821 tgtacctgaa ggacaaggaa gccgccttgg aaatactcgg acccctgttc gaaacgatct
     8881 ctgattcatt tttcccttat gcgaaggccg acccggatct cgcgtcgctg cgcgacgagc
     8941 cgcgttatca agccatggtc gccgttgccg aggcgcgctt ggcggcgtcg aagaccgcgg
     9001 cccccgaaat ggttgccggg agctgacggc aatggccgac gtcttcgttt cctacgcccg
     9061 cgcagacaag gcacgtgtcg cgccgctggt ggccgcgatc gaggccaagg gctggtcggt
     9121 gtggtgggat ccggagatca gtgcgggcca ggaattcgat gatcagatcg aagccgagat
     9181 cgatgcggcc agggcggtgc tggtggtatg gacgccgact tcggtcgcgt cacgctgggt
     9241 tcgcggcgaa gcgcgcgaag cagccgagcg cggcatcctg gtgccggtac gcttcgagca
     9301 ggcccgcctg ccgatggatg tgcgcgcgat ccatacgacc gacttggacg cgtggggcga
     9361 ggattcgacg agtccactga tgcaggagtg cttgcacgcg ctcggcgcga tgatcgcgcg
     9421 ctcgcagatt gcacaggccg cgaaggatgc gagtgcgacg tcggttaccc ccaagtcgtc
     9481 aaaaaatgct gcgcgctttt cgatctgcgt cttgccgttc gtcaacatga gtggtgatcc
     9541 ggaacaggaa tatttcagtg acgggatcac cgaggacatc atcaccgacc tcagcaaggt
     9601 ctcggcgctg gaagtggttt cgaggaacgg ttcgttcctg tacaagggca agcaggtcga
     9661 cgtgcccaag gttgcgcacg aactcaaggt cacgcatgtg ctggagggca gcgtgcgcag
     9721 ggctggcggt cgggtgagga tcagcgcgca gttgatcgac ggtgcccgaa atcggcacgc
     9781 gtgggcggag cgttacgacc gcgatgcgag cgatatcttc gcgctgcaag acgagatttc
     9841 ccacgccatc gtcaaggcac tgcggctgca gttgctgccg gaagagaaga aggctatcga
     9901 acgacgcggt accgacagcg tggaagcgca tgacctgtat ttgatggcac ggcagctcta
     9961 cgttaccagc caggacgacg agcacgcatc gagagcgatc gtgcgcgcct gtgcgcgcgc
    10021 gacgcaaatc gacccgaatt acgcgcaggc gtgggcgctg aaagcggtgg gcgcgttgaa
    10081 tctttgcgat cttggcctcg aaaccgatga tggcatggcg gccgcagaga gggcgcttga
    10141 actggatccc gacctggtcg aagcgcacgc ggtaaagggt tacattttgc ggatgcgcgg
    10201 caacatggag ggggccatca ccgaagcctc gatcgccatg aaactggatc cggagtcgta
    10261 tgaggccaac cgtgccgccg cccggatcag ctacgtgcag catcgattcg aggatgctgt
    10321 ccgcctttat gagaaggcgg tgagcttgat ggattcggat gtcaattcag cgatgctgct
    10381 ggttagttcg tatcgtgcgc tcggggacgt agtgggtatg cgacgcgccg cggaggttgc
    10441 attgaagcgt gcggacgcgg tgctggcgcg cgaccagagc aattcttacg tgcttgccta
    10501 tagcgccaat gccctcggcg ccctgggcga gggcgaacga gccaaggcgc gcatgagccg
    10561 cgcgatgctg atcgaccccg acaactggaa catgcgctac aacttcgcat gcaccctcag
    10621 catgtacctg caagatactg acgcggcgtt ggagatcctg gagtccttgc tcgatacggc
    10681 aaccggatcc ctgctcgact acataaagga cgatcccgac ctggattcct tgcacgacaa
    10741 cccgcgctat gtcgccatgg tcgcggcgac tgaggcgcgc ctcgccgcgg cgaagcagga
    10801 tgaagcgccg gcgctgcaga aagcctgagc gccattcggt gagccggttc gcggcgccaa
    10861 gccccgccac agcgcgcgag gcgctatgat cggtagacgg gcgtctgtac gtccggttgg
    10921 tggggtgcat ctgcatggct gacgtcttca tttcctacgc ccgggccgac aaggcgcggg
    10981 tcgcgccctt ggtggccgcg atcgaggcca agggctggac ggtgtggtgg gatccggaaa
    11041 tcgcgccagg ccgccagttc gacgacgaga tcgacgtcgc gcttcaggct gcgaaagccg
    11101 tgctggtggt ttggacgccc acctccgtgg tttcgcgctg ggtgcgcggc gaggcgcgcg
    11161 atgccgccga gcgcgggatc ctggtgccgg tgcgcttcga ccaggcgcgc ctgccgatcg
    11221 atgtacgcgc catccacacc acggatctcg atggctggca cgaggacccg gcgcatccgg
    11281 cggtgcagga atgcctgcat gcgctggacg cgatgatcgc gcgggcaccg gccgatgcag
    11341 gcgaggccgc gaggggctcg gcaccggccc ctggcaaacc tgcgccacgc ttttccattt
    11401 gcgtgctgcc gttcgtcaat atgagcggtg acccggaaca ggaatatttc agtgacggca
    11461 tcaccgagga catcctgacc gacctcagca agatctcggc gctgtcgatc atctcgcgca
    11521 acggttcgtt cctgtacaag ggcaagcagg tcgacattcc gcagatcgcg cgcgacctca
    11581 acgtcacgca tgtgttggaa ggaagcgtac gcaaggctgg cgggcgggtc cggatcagtg
    11641 cgcagttgat cgacggcacc gccaacaacc acgtgtgggc cgaacgctac gaccgcgacg
    11701 ccagcgacat cttcgcgctg caggacgaga tttcgcacgc gatc
//
LOCUS       DASSNH010000276         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_417277, whole genome shotgun sequence.
ACCESSION   DASSNH010000276 DASSNH010000000
VERSION     DASSNH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417277"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VFK00_10110"
     CDS             complement(<1..146)
                     /locus_tag="VFK00_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7593346.1"
                     /translation="MALKTLKPTSPGRRGTVRVSTPDLHKGAPYAALTEAQSKTGGRN
                     HHGR"
     gene            complement(165..461)
                     /gene="rplW"
                     /locus_tag="VFK00_10115"
     CDS             complement(165..461)
                     /gene="rplW"
                     /locus_tag="VFK00_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7593347.1"
                     /translation="MNKQERILDTLRAPLVSEKTALLSASNQYAFEVAADATKADVKA
                     AVEQLFDVTVERVNMVNIHGKSKSFRFRQGTRQGKRKAYVRLAEGQTIDVMAKA"
     gene            complement(458..1060)
                     /gene="rplD"
                     /locus_tag="VFK00_10120"
     CDS             complement(458..1060)
                     /gene="rplD"
                     /locus_tag="VFK00_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010371054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7593348.1"
                     /translation="MELNVIGAKPLEVSDVVFGGEFRENLVHQVVVAYQAGGRAGTKA
                     QKTRSEVNGTTKKSKRQKGGGARHGALTAPIFVGGGVTFAAKPRNFEQKVNRKMYRGA
                     LRSILAELNRQGRLKVVDSFGIDAPKTSAMVAKLGELEASGRVLLVDENASEALYLAA
                     RNIPNVYVVDKAALDPASLVHADHVVMTTESVKKIEEWLA"
     gene            complement(1071..1712)
                     /gene="rplC"
                     /locus_tag="VFK00_10125"
     CDS             complement(1071..1712)
                     /gene="rplC"
                     /locus_tag="VFK00_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7593349.1"
                     /translation="MSIGLVGRKCGMSRIFTEDGQSIPVTLIEATPNRVAQVKTDETD
                     GYSAVQVTAGTRRASLLTKPQKGHYAKAKVEPGRGLWEFRVSAEDAGKYEVGGEIKAE
                     DVFQAGQIVDVAGVSKGKGFQGTIKRWNFSMGDATHGNSLSHRAPGSIGQRQTPGRVF
                     PGKKMSGHMGAVNRTQQGLEVVKVDAERHLIAVKGAVPGAAGGDVIIRPTTKG"
     gene            complement(1733..2044)
                     /gene="rpsJ"
                     /locus_tag="VFK00_10130"
     CDS             complement(1733..2044)
                     /gene="rpsJ"
                     /locus_tag="VFK00_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7593350.1"
                     /translation="MANQKIRIRLKAYDHRLIDRSASEIVETAKRTGATVLGPIPLPT
                     KIERYTILTSPHVDKDARDQYETRTHKRVLDIVDPNDKTVDALMKLDLAAGVDVQIKL
                     G"
     gene            complement(2193..3383)
                     /gene="tuf"
                     /locus_tag="VFK00_10135"
     CDS             complement(2193..3383)
                     /gene="tuf"
                     /locus_tag="VFK00_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7593351.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAALTKIGAERFGGEF
                     KAYDQIDAAPEEKARGITISTAHVEYESPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFMNKADMVDDAELLELVEMEIRELLSK
                     YNFPGDDTPIIKGSALKALEGDQSEIGVPAILKLVEALDAYIPEPVRDVDKPFLMPVE
                     DVFSISGRGTVVTGRIERGIIKVGDEIEIVGIRPTTKTTVTGVEMFRKLLDQGQAGDN
                     AGLLLRGTKRDEVERGQVLAKPGSITPHTKFEAEVYVLSKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGAVELPEGVEMVMPGDNVKMKVTLIHPIAMEEGLRFAIREGGRTVGAGVVA
                     KINK"
     gene            complement(3425..3500)
                     /locus_tag="VFK00_10140"
     tRNA            complement(3425..3500)
                     /locus_tag="VFK00_10140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3465..3467),aa:Thr,seq:ggt)
     gene            complement(3537..3610)
                     /locus_tag="VFK00_10145"
     tRNA            complement(3537..3610)
                     /locus_tag="VFK00_10145"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3576..3578),aa:Gly,seq:tcc)
     gene            complement(3683..3768)
                     /locus_tag="VFK00_10150"
     tRNA            complement(3683..3768)
                     /locus_tag="VFK00_10150"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3732..3734),aa:Tyr,seq:gta)
BASE COUNT          634 a         1316 c         1153 g          807 t
ORIGIN      
        1 atgcggccgt gatgattgcg accaccggtc ttggactgcg cctcggtcag cgccgcgtac
       61 ggcgcgccct tgtgcagatc cggcgtggat acccgaacgg taccacggcg tcccggagag
      121 gtcggcttga gtgttttcaa tgccattgtc gttcaactcc tcgctcaggc cttggccatc
      181 acgtcgatgg tctgaccttc agccaggcgc acataggctt tgcgcttacc ctgacgggta
      241 ccctggcgaa aacggaaaga cttgctcttg ccgtggatat tgaccatgtt cacccgctcg
      301 acggtcacgt cgaacagctg ctccaccgcc gccttgacgt cggccttggt cgcatcagcg
      361 gcaacttcga aagcgtactg gttggaagca ctcagcaacg ccgttttttc cgacaccagc
      421 ggcgcgcgca gggtatcgag aattcgttcc tgcttgttca tgccagccac tcctcgatct
      481 tcttgacgga ttcggtcgtc atgacgacgt gatccgcatg caccaggctt gccggatcca
      541 gcgccgcctt gtccaccacg tacacgttgg ggatgttgcg tgcggccaga tacagggctt
      601 cgctggcatt ttcgtccacc agcagaacgc ggccgctggc ttccagttca cccagcttgg
      661 cgaccatcgc gctggttttc ggtgcatcga tgccgaagct gtcgaccacc ttcagacgac
      721 cctgacgatt cagctcggcc aggatcgagc gcagggcgcc gcgatacatc ttgcgattga
      781 ccttctgctc gaagttgcgc ggcttggctg caaaggtcac gccgccgccg acaaaaatcg
      841 gcgccgtcag cgcaccatgg cgagcgccgc cacccttctg gcgcttggac ttcttggtcg
      901 taccattcac ttccgagcgc gttttctgcg ccttggtgcc ggcacggccg ccggcctgat
      961 aggcgaccac gacctgatgc accaggtttt cgcgaaattc gccgccaaac accacgtcgg
     1021 aaacctcgag cggcttggcg ccaatgacat tcagttccat gccgatctcc tcaacccttg
     1081 gtcgtcgggc ggatgatgac gtcgccgccg gcagcacccg gcaccgcgcc cttcaccgcg
     1141 atcagatgcc gttcggcatc gaccttgacc acttccagac cctgctgggt acggttgacc
     1201 gcacccatgt ggcctgacat tttcttgccc gggaacacgc ggcccggcgt ctgacgctga
     1261 ccgatggaac ccggcgcgcg atgcgacagc gagttgccgt gcgtggcgtc gcccatggag
     1321 aagttccagc gcttgatggt gccctggaaa cccttgccct tggacacgcc ggccacgtcc
     1381 acgatctggc ccgcctggaa cacgtcctcg gccttgatct cgccgccgac ttcatacttg
     1441 ccggcgtctt cggcactgac gcggaattcc cacaggccgc ggcccggctc cactttcgcc
     1501 ttggcgtagt ggcccttctg cggcttggtc aacagcgagg cgcgacgcgt acccgcggtc
     1561 acctgcacgg cgctgtagcc atctgtctcg tcggtcttca cctgggccac acggttcggc
     1621 gtcgcttcga tcagcgtcac cggaatcgat tggccgtctt cggtgaagat ccggctcatg
     1681 ccgcatttgc ggcctaccag tccgatactc atcttcgtat cctgcctttg gctcagccga
     1741 gcttgatctg gacgtcgaca ccggcggcca ggtcaagttt catgagcgcg tccacggtct
     1801 tgtcgttggg atccacgatg tcgagcacac gcttgtgcgt gcgggtctcg tactggtcgc
     1861 gggcatcctt gtcgacgtgt ggcgaagtca aaatggtgta acgctcgatc ttggtcggca
     1921 gcggaatcgg accgagaacc gtggcgccgg tgcgcttggc ggtctcgacg atctcgctgg
     1981 cggagcgatc gatcaagcgg tgatcatagg ctttgagcct gatgcgaatc ttctggttcg
     2041 ccataaccgt gtaccttgct aaagaacgtt gttttgcaca tttccggcag tgtctcgccg
     2101 ccggcgttta tgtcgccagg agcgagggac gaggggtggg cagcgagagt ggtacttgcc
     2161 aacctcgcct ctcgtccctc gcccccaatc cctcacttgt tgatcttggc caccacgccg
     2221 gcgccgacgg tgcggccgcc ctcgcggatg gcgaagcgca ggccttcttc catggcgatc
     2281 gggtggatca gcgtgacctt catcttcacg ttgtcgcccg gcatcaccat ttccacgcct
     2341 tccggcagct cgaccgcgcc ggtcacgtcc gtggtgcgga agtagaactg cgggcggtag
     2401 cccttgaaga acggcgtatg acgaccgccc tcctccttcg acagcacgta cacctccgcc
     2461 tcgaacttcg tgtgcggcgt gatcgacccc ggcttcgcca gcacctggcc gcgctcaacc
     2521 tcgtcgcgct tggtgccgcg cagcagcagg cccgcgttgt cgcccgcctg gccctggtcc
     2581 agcagcttgc ggaacatctc cacgcccgtc accgtcgtct tggtggtcgg acggatgccg
     2641 acgatctcga tttcgtcgcc caccttgatg atgccgcgct cgatgcggcc ggtcaccacc
     2701 gtgccacgac ccgaaatcga gaacacgtcc tcgaccggca tcaggaacgg cttgtccacg
     2761 tcacgcaccg gctccgggat gtaggcgtcc agcgcctcca ccagcttcag gatcgccggc
     2821 acgccaatct ccgactggtc gccttccagc gccttgagcg ccgaaccctt gatgatcggg
     2881 gtgtcgtcgc ccgggaagtt gtacttcgac agcagctcgc ggatctccat ctcgacgagc
     2941 tccagcagct cggcgtcgtc gaccatgtcc gccttgttca tgaacaccac gatgtacggc
     3001 acgcccacct gacgggccag caggatgtgc tcgcgcgtct gcggcatcgg gccgtccgcc
     3061 gccgacacca ccaggatcgc accgtccatc tgcgccgcac ccgtgatcat gttcttcacg
     3121 tagtcggcgt ggcccgggca gtccacgtgc gcgtagtgac gcgtcgggct ctcgtactct
     3181 acgtgcgccg tcgagatcgt gatgccgcgc gccttctctt ccggcgccgc gtcgatctgg
     3241 tcgtacgcct tgaactcacc gccgaatcgc tccgcgccga tcttcgtcag cgccgccgtc
     3301 agcgtcgtct tgccgtggtc aacgtgacca atcgtgccga cgttcacgtg cggcttcttg
     3361 cgctcgaact tttcctttgc catggctatc tctcgctgac gttaggtgct tatcgacgac
     3421 aaaatggtgc tcacgaatgg aatcgaacca tcgacctcct ccttaccaag gaggtgctct
     3481 accgactgag ctacgtgagc gtgttttcga ccaagcagga ccgtggacat caatggtgga
     3541 gcgggagacg ggaatcgaac ccgcgctagt agcttggaag gctacagttc taccattgaa
     3601 ctactcccgc gccgggaggc ctgcccttgt cgcgccggag gctggtaagg ccgccgggta
     3661 ccgcatcttg gaaccgtgga gctggtggag ggaggtggat tcgaaccacc gaaggcgtaa
     3721 gccagcagat ttacagtctg cccccgttgg ccgcttgggt atccctccaa aagagcccgc
     3781 aattttgacc gcgcccgcgc ggactgtcaa cggccggcgc gggggaattt cgcttgccgc
     3841 gccggtcgcg cgcgccgggt cacacgttga agcggaaatg caggacgtcg ccctcctgca
     3901 cccggtactc
//
LOCUS       DASSNH010000277         9263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_491080, whole genome shotgun sequence.
ACCESSION   DASSNH010000277 DASSNH010000000
VERSION     DASSNH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491080"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..700
                     /locus_tag="VFK00_10155"
     CDS             362..700
                     /locus_tag="VFK00_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7593352.1"
                     /translation="MKLITAIIKPFKLDDVREALSEVGVNGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEIALPDNLVERAVEAISHAARTGKIGDGKIFISTLDQVMRI
                     RTGELDADAL"
     gene            complement(812..1342)
                     /locus_tag="VFK00_10160"
     CDS             complement(812..1342)
                     /locus_tag="VFK00_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1453 domain-containing protein"
                     /protein_id="HET7593353.1"
                     /translation="MSPNLIAPIVIAPLVVWQMYYRMRRNFGRQPIRPGRMWTRVGLL
                     CGVSMLVALAGIRDPRLAEGLALGLLGGAVLGIVALRLTRFEIDGVNDCYFPNVWIGL
                     ALTTLLVGRLLYRFMVAWPGMSNVAAGGAYASLQHSPLTLGIVGLLFGYYIAYYAGLL
                     LHHRRARMTIASNQPS"
     gene            complement(1401..2213)
                     /gene="mutM"
                     /locus_tag="VFK00_10165"
     CDS             complement(1401..2213)
                     /gene="mutM"
                     /locus_tag="VFK00_10165"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET7593354.1"
                     /translation="MPELPEVVSTRRGIDPFVTGRVVDRVLLRRRDLRWPIPRPLTAK
                     LPGQRIDAVERRAKYLLLHTEAGSALLHLGMSGSLRITDPALPPRMHDHYDIVLDSGK
                     ALRFNDPRRFGCLLWQKPGEVHPLLQGLGPEPLGDAFDGDGLWRASRGRTAAVKTFLM
                     DQSIVVGVGNIYASEALFAAGVHPKRAAGGVSRARYARIAAEIRRVLAYAITRGGTTL
                     RDFLAPDGAPGYFEQELAVYGRAGEPCRVCGTPIRSTVVGQRMTYWCPRCQR"
     gene            complement(2275..2772)
                     /locus_tag="VFK00_10170"
     CDS             complement(2275..2772)
                     /locus_tag="VFK00_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET7593355.1"
                     /translation="MTRATRNLSVLALVAVVLVAAVLWVGRRDYIRNPPTLTPIDPAN
                     VTHIGLDIPPIAAQAFEKRHGGWWRAQPSAARADDARVDRLAKLAATPVARWIPRTDV
                     EPSDIGLERPSATLTLDGVQLEYGGLTALDDLRYVRVGDKIALVPRQYSPEAMLTRRA
                     GETTP"
     gene            complement(2769..4100)
                     /locus_tag="VFK00_10175"
     CDS             complement(2769..4100)
                     /locus_tag="VFK00_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET7593356.1"
                     /translation="MRHAWQGWLHALLCVVIAVCIAALSTHFSATVDWSAGARATIPA
                     QSRALLAQLEGPVTVVAYARPGALRAKTRLLVERYQRFKPDLELKFVDPDLDPTATED
                     AGIQGDGELVLHWHDRTQHVTQLGEQGFADALARLARGGTRLVAFVTGDGERDPTGKN
                     AADLGAFVARIAHRGVRALPLNLAEAAEVPRNADLVVLASPQAALLPGAVKKLEDYVA
                     AGGNLLWLTEPGGDDLGLAPLAQMLGIRRLPGTLVDPQGAAVGVRDPRAIVATRYPAQ
                     AITDGFPLNTLFPRVAVLASMAGGDWKAQPILQSSARSWNRNLPDDPATGVLDPVADA
                     LKGPLSFGYALSRLSPSPAKTEQRVVVIGDGDFLSNAYLAEAGNLALGERVIDWLLGD
                     DAMASLPPAAPDTSLKPTRAELGVLTYGYLVGLPIVLILIGITITWRRRRR"
     gene            complement(4100..4855)
                     /locus_tag="VFK00_10180"
     CDS             complement(4100..4855)
                     /locus_tag="VFK00_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7593357.1"
                     /translation="MTAPAIARLDLKRLAVQPFPWVLAGIVVAMMAWQFLLGLSAFLH
                     AQPTVPGATAATGFTDGVIGPYFLNYLQLCLVIAPLVTMQALAGERGEQRLALLGAAG
                     ASGFQIVAGKFLARLAFLWLLLVVVASMPLVLGGATRIDWGQFAAALVATALCVAALT
                     AIGIATSAYAPGPALAAAAALLIGEALSLIDAGVRLAGGGGGWLGYLALHTHLAPALR
                     GLMRSEDIVYFLLVTALALALAMRRIGAERERG"
     gene            complement(4852..5586)
                     /locus_tag="VFK00_10185"
     CDS             complement(4852..5586)
                     /locus_tag="VFK00_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7593358.1"
                     /translation="MAEEGALLQLDRISRRLGGRSVLVDVSFTLQRGEVLGLLGVNGA
                     GKSTTLAIIAGVLTPDTGSVRIDGRDLADDVRGLRGRIGWVPESVPLWPELNLTEALD
                     ASGRLRGMTRAARRSAIERELRRFELTGLARRLCGQLSLGQKRRAGLAQALLHEPDLL
                     VLDEPGNGLDPVQAAQLREAIGSLSPDRGIILSSHDLAEVEAMCSRVVVLHEGSVRQD
                     ARLGGERGWLDAQFRAIAGSPASAAA"
     gene            5733..6935
                     /locus_tag="VFK00_10190"
     CDS             5733..6935
                     /locus_tag="VFK00_10190"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET7593359.1"
                     /translation="MLETFLDFASNGLAHAGWLTMLLYVLVTTQLTIFAVTLYLHRSQ
                     AHRGVDFHPAVAHILRFWNWISTGMITREWIAVHRKHHAHCETEQDPHSPQVAGIRKV
                     FFEGSELYRAAAANEADLEKYGRGAPDDWMERHVYSAHPYLGVVLLATIDVVLFGALG
                     GAIWAVQAAWIPFWAAGVVNGLGHWWGYRNFETADKATNLTPWAVWCGGEELHNNHHA
                     FPSSAKFALRRFEVDIGWLVIRGLEKMRLAKVLRVAPALDERPNVSLPDMVTLKALLT
                     HRFEAMTEYYRMVIKPTVREQSSAEGTRRLGAMPRRRLRRALANGGRWLDKTGKARLA
                     AVVENRPRLKQIAEYRARLAALMEQRGKPESTLAALQQWCREAEASGNRKLIEFAARL
                     KRYSVARA"
     gene            7161..8138
                     /locus_tag="VFK00_10195"
     CDS             7161..8138
                     /locus_tag="VFK00_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593360.1"
                     /translation="MTALWHPAIRADWLVLRRTGAVAFARLIGRPALLLACCAAIACG
                     DFYGGYGWCLVAGALLALGGSHAIAHQQLLGAAERWRFGWCGALPVARGTTAGTLLLV
                     ATAALMVSVVFVTALLLGVSMPAPHRGDLPYALAAIDLGLVFGTASAAVRVFRRGAIA
                     RARHADGIREPLFPLPWLNDPRLPHLLDWQRRAALVRWRRGGSFAMVGVALAAVPRGP
                     SLPMVVALMLLVLSWSWLAVVMRASADASTAAIRLLGATPLDVRHAFLASLRYPVMAA
                     SCAVVPMAVGATLGGRSVIALAWLVCAGAISAWPILRILRATRSPDASA"
     gene            8135..8863
                     /locus_tag="VFK00_10200"
     CDS             8135..8863
                     /locus_tag="VFK00_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7593361.1"
                     /translation="MTALLRAADLCAAYGDTRVLRGVDVQLEHGEFVALIGPNGSGKT
                     TLLHALAGLVPLSGGSVRIGGYDLAKAPRRAKAVFGLAVDPARLPALLTGRECLALFA
                     GARGFPAIPPATFALGETLSLTPVLDRRIDGYSLGMRQKLAILLGLLGEPPLLLLDEP
                     FNGLDPRSALAFKRHLAALVARGDTGVLLATHSLDTAERHATRVILLMDGRVRHVWNA
                     AELLALRENREHSLELAMAEVCGT"
     gene            8955..>9263
                     /locus_tag="VFK00_10205"
     CDS             8955..>9263
                     /locus_tag="VFK00_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593362.1"
                     /translation="MTKRRASIAVLTLCLCASVLPASAQTTTKLPRITVTGTPYVEHH
                     GGYVISGNFKVDPRMPYVVFPARALVEDDILSVHPVHLNDDDYLVLQECVASDCSRAH
                     L"
BASE COUNT         1449 a         3171 c         3158 g         1485 t
ORIGIN      
        1 gcaatccgtg cgccagcacg tcccgaaagt tggcttccat gtcttcccgc gcctgcgcca
       61 gccccggagg aaccagggtc gccagccgat gcgcgagttg gtctatggag tgcacgtcaa
      121 tcatgtcgtc gcctcgatgc gtccgcagct tacggcgccg gcaacggcgc gaatatcggc
      181 ggacgtcttg tagtggtgta gggattttcc tacacgtgga tgtgctcgtc catgaacgcg
      241 gcttcggtaa ccggcaggtg ggcgccatcg ccaggcgcca gaacggcctt tcggctccgg
      301 cctggacagc ggcatttcca gcggtgattc tgcagcccgt ctctccggta agcttgcggc
      361 catgaaactg atcaccgcca tcatcaagcc gttcaagctc gacgacgtgc gcgaggcgtt
      421 gtccgaagtc ggcgtcaacg gcatcaccgt caccgaggtc aagggcttcg ggcgccagaa
      481 gggccacacc gaactctacc gcggggccga atacgtggtc gatttcctgc ccaaggtgaa
      541 gctcgagatc gcgttgcccg acaacctcgt cgaacgggca gtggaagcca tcagccatgc
      601 tgcgcgaacc ggcaagatcg gcgacggaaa gatcttcatc tcgacgctcg accaagtgat
      661 gcgcatccgg acgggcgagc tcgacgctga cgcgctgtag gtgcaatcaa tccctgattg
      721 cggcctcgat gcagtgagtc gcattgggaa gcgcgtttcg cgcaacggtc accagatcgg
      781 ccgtccatgg cccgactttc cgcggaagct tttacgaggg ttggttcgag gcgatggtca
      841 tccgcgcgcg acggtggtgc agcaacagcc ccgcgtagta agcgatgtag tagccgaaca
      901 gcaacccgac aatgccgagc gtcaaggggc tgtgctgcag gctcgcatag gcgccgccag
      961 ccgccacgtt cgacatcccg ggccaggcca ccatgaagcg gtagagcaac cggccgacca
     1021 gcagcgtggt gagcgccaag ccgatccaca cgttggggaa atagcaatcg ttgaccccgt
     1081 cgatctcgaa acgcgtcagc cgcagcgcga cgatccccag caccgcgccg ccgagcagcc
     1141 ccagcgcgag cccttcggcg aggcgtggat cgcggatgcc tgccaacgcg acgagcatgg
     1201 ataccccgca cagcaatccc acgcgtgtcc acatccggcc ggggcggatt ggctggcgcc
     1261 cgaagttgcg gcgcatccgg tagtacatct gccacacgac cagcggggcg atcacgatcg
     1321 gagcgatgag attgggcgac atggcaaacg gctccgacgg ctgcggccgg cctattctgg
     1381 cgcaacggct acgtgcgcgc ctacctttgg cagcgcgggc accagtacgt catgcgctgc
     1441 ccgaccaccg tcgagcggat cggcgtgccg cacaccctgc acggttcgcc ggcgcggccg
     1501 tacaccgcga gttcctgctc gaagtagccc ggggcgccgt cgggcgccag gaaatcccgc
     1561 agggtggtgc cgccgcgcgt gatcgcgtac gcgaggactc ggcggatttc cgcggcgatg
     1621 cgcgcgtatc tggcgcgcga cacaccgccc gcggcgcgct tcgggtgcac gccggccgcg
     1681 aacaaggctt cgctggcata gatgttgccg acgccgacca cgatcgactg atccatcagg
     1741 aaggtcttca ccgccgcggt gcgtccgcgc gacgcgcgcc acaacccgtc gccgtcgaac
     1801 gcatcgccga ggggttcggg gcccagtccc tgcagcagcg gatgcacttc gcccggcttt
     1861 tgccaaagca ggcaaccgaa ccggcgcggg tcgttgaagc gcagggcctt gcccgagtcg
     1921 aggacgatgt cgtagtggtc gtgcatgcgc ggcggcagcg ccgggtcggt gatgcgcagc
     1981 gagccggaca tcccgaggtg cagcaatgcg gaaccggctt cagtgtgcag caacaggtat
     2041 ttcgcgcggc gttcgacggc atcgatccgc tggcccggca gcttcgccgt gaggggccgc
     2101 gggatcggcc agcgcaggtc gcgccttcgc aacaacacgc ggtccaccac gcgccccgtc
     2161 acgaagggat cgatgccgcg gcgcgtgctt acgacttcgg gaagttcggg catgttgcga
     2221 ggtcaagtcg atcggatcga cgccggactt gtccggcgga agcccaaccg gaagctatgg
     2281 cgtcgtttca cctgcgcgcc tggtgagcat cgcttccggc gaatattgcc gcggcaccag
     2341 ggcaatcttg tcgccgaccc gcacgtagcg caggtcgtcg agggcggtga ggccgccgta
     2401 ttcgagttgg acgccgtcga gggtcaaggt tgccgagggg cgttccagcc cgatgtcgga
     2461 cggctcgacg tccgtacgtg gaatccagcg cgcaacgggc gttgcagcca gcttggccaa
     2521 tcgatcgacg cgcgcatcgt cggcgcgcgc ggcggagggt tgcgcccgcc accagccgcc
     2581 gtggcgcttc tcgaaggcct gcgctgcgat cggcgggatg tcgaggccga tgtgcgtgac
     2641 atttgccgga tctatcgggg tcaatgtcgg cggattgcga atgtaatcgc ggcgtccgac
     2701 ccacagtacg gccgcgacga gcaccaccgc gaccagcgcg aggaccgaga ggttgcgcgt
     2761 ggcgcgggtc atcgacggcg ccggcgccag gtgatcgtga tgccgatcag gatcagcacg
     2821 atcggcaggc cgaccagata cccgtacgtc agcacgccga gttccgcacg ggtcggcttg
     2881 agcgaggtgt cgggggcggc gggcggcagg ctcgccatgg catcgtcgcc gagtagccag
     2941 tcgatgacgc gctcgccgag tgcgaggttg ccggcctcgg ccaggtaggc gttcgaaagg
     3001 aaatcgccgt cgccgatcac caccacccgc tgctcggtct tcgccggact gggcgatagc
     3061 cggctgagcg catagccgaa gctgagcgga cccttgagcg cgtcggcgac gggatcgagg
     3121 acgccggtcg ccgggtcatc cggcaggttc cggttccagc tccgcgcgct cgactgcagg
     3181 atcggctgtg ccttccagtc gccaccggcc atcgacgcca gcaccgcgac gcgcgggaac
     3241 agcgtattca acggaaagcc gtcggtgatc gcctgcgccg ggtagcgggt cgcgacgatg
     3301 gcgcgcgggt cgcgcacgcc gaccgcggcg ccctgcggat cgacgagcgt cccgggcagg
     3361 cggcggatgc cgagcatctg cgccagcggc gcaagtccca ggtcgtcgcc acccggctcg
     3421 gtcagccaga ggagattccc gccggccgcg acatagtcct ccagcttctt caccgcgccc
     3481 ggcaggagcg ccgcctgcgg gctcgccagc accacgagat cggcattgcg cggcacctcg
     3541 gccgcctcgg ccaggttcaa cggcagcgcg cgcacgccgc ggtgggcgat ccgcgccacg
     3601 aaggcgccga ggtcggcggc gttctttccg gtggggtcgc gctcgccgtc gccggtgacg
     3661 aacgccacca gccgggtacc gccgcgcgcg agccgcgcca gtgcatcggc gaagccctgc
     3721 tcgccgagct gtgtgacatg ctgcgtgcga tcgtgccaat ggagcacgag ctcgccgtcg
     3781 ccctggatgc cggcatcctc ggtcgcggtc ggatcgaggt ccggatcgac gaacttcagt
     3841 tcgaggtccg gcttgaaccg ttggtaacgt tccaccagca ggcgcgtctt ggcgcgcagc
     3901 gcaccgggcc gcgcgtacgc caccaccgtg accggcccct ccagctgcgc gagcagcgcg
     3961 cggctctgcg cggggatcgt cgcgcgggcg ccggcgctcc agtcgacggt ggcgctgaag
     4021 tgcgtcgaca gcgcggcgat gcacaccgcg atcaccacgc acagcagcgc gtgcaaccag
     4081 ccttgccagg catgccgcat cagccgcgct cccgttccgc gccgatgcgg cgcatcgcga
     4141 gcgccaatgc cagcgccgtg accagcagga agtacacgat gtcctcgctg cgcatgaggc
     4201 cgcgcagtgc cggcgccagg tgggtgtgca gcgcgaggta gccgagccag ccgccaccgc
     4261 cgcccgcgag ccgcacgccg gcgtcgatca gcgacagcgc ctcgccgatc agcaacgcgg
     4321 ccgccgcggc cagcgcaggg ccgggtgcgt acgccgaagt cgcgatgccg atcgccgtca
     4381 gcgccgccac gcacagcgcc gtcgcgacga gggccgcggc gaattggccc cagtcgatgc
     4441 gggtcgctcc gcccagcacc agcggcatgc tcgcgaccac gaccagcagc agccacagga
     4501 acgccagccg tgcgaggaac ttgccggcga cgatctggaa gcccgaggcc ccggccgctc
     4561 cgagcagggc gagacgctgc tcgccgcgct caccggcgag cgcctgcatc gtcaccagcg
     4621 gcgcgatcac gaggcacagt tgcaggtagt tgaggaagta cggcccgatc acgccgtcgg
     4681 tgaaaccggt cgccgcggtc gcgccgggga ccgtcggctg cgcatgcagg aacgccgaaa
     4741 gtccgagcag gaactgccac gccatcatcg ccacgacgat gccggcgagc acccacggga
     4801 acggttgcac ggcaaggcgt ttcaggtcca ggcgcgcgat cgcgggcgcg gtcatgcggc
     4861 ggcgctcgca ggtgaaccgg cgatcgcgcg gaactgcgca tcgagccatc ctcgctcgcc
     4921 gccgagccgc gcatcctgcc gcaccgaacc ttcgtgcagc accaccacgc ggctgcacat
     4981 cgcctcgact tccgcgaggt cgtggctgga gaggatgatg ccccggtccg gcgaaaggct
     5041 gccgatggcc tcgcgcaact gcgctgcctg caccggatcc aggccgttgc cgggttcgtc
     5101 gaggaccagc aggtccggct cgtgcagcag cgcctgcgcc aatccggcgc gccgcttctg
     5161 ccccagcgaa agctgcccgc acagccgcct ggccaaaccc gtcagctcga accgccggag
     5221 ttcgcgttcg atcgccgagc ggcgggcggc gcgcgtcatg ccgcgaagcc gacccgaggc
     5281 atccagcgct tcggtgaggt tcagctccgg ccacagcggc acgctttcgg gcacccatcc
     5341 gatgcggccg cggagcccgc ggacgtcgtc cgcgagatcg cgcccgtcga ttcgcaccga
     5401 cccggtgtcg ggcgtgagaa ccccggcgat gatcgccagc gtcgtcgact tgccggcgcc
     5461 gttcacgccc agcagcccca gcacctcccc ccgctgcagg gtgaacgaca cgtcgaccag
     5521 gacgctccgg ccgcccagcc ggcggctgat gcggtcgagc tgcagcaacg ccccctcttc
     5581 ggccatttcg ttagatcgtt gtaaaaagca ttgaaagaat tgtcaccgcc atcacatact
     5641 tctagcaagc cggtagcgac gagcagggtt ctcccctaga ctcctcgatg cgggtttgtc
     5701 ccgaccccgt gccccttcct cgcgtgaccg agatgctcga aacctttctt gattttgcct
     5761 cgaacggcct ggcgcacgcc ggctggctca cgatgctgct gtacgtgctg gtcacgaccc
     5821 agctcacgat tttcgccgtc accctgtacc tgcaccgcag ccaggcgcat cgcggggtgg
     5881 atttccaccc agccgtcgcg cacatcctgc gtttctggaa ctggatcagc accggcatga
     5941 tcacgcgcga atggatcgcg gtgcaccgca agcaccacgc ccattgcgag accgagcagg
     6001 atccgcacag cccgcaggtc gccggcatcc gcaaggtgtt cttcgagggc tccgaactct
     6061 accgcgccgc ggcggcgaac gaggccgacc tcgagaagta cggccggggc gcgcccgacg
     6121 actggatgga gcggcacgtc tattcggcgc acccgtacct cggcgtggtg cttcttgcga
     6181 ccatcgacgt ggtgttgttc ggcgcgctgg gcggcgcgat ctgggcggtc caggcggcgt
     6241 ggatcccgtt ctgggccgca ggcgtcgtca acggcctcgg ccactggtgg ggctatcgca
     6301 atttcgagac cgccgacaag gccaccaacc tcacgccctg ggcggtgtgg tgcggcggcg
     6361 aggaactcca caacaaccac cacgcgttcc cgagctcggc gaagttcgcg ctgcgccgct
     6421 tcgaggtcga catcggttgg ttggtgatcc gcggcctcga gaagatgcgc ctggcgaagg
     6481 tcctgcgcgt cgccccggcg ctcgacgagc gtccgaacgt gtcgctcccg gacatggtga
     6541 ccttgaaggc cctgctcacc caccggttcg aggccatgac cgagtattac cggatggtga
     6601 tcaagccaac ggtgcgcgag cagtccagtg ccgaaggcac gcgccggctg ggtgcgatgc
     6661 cgcggcggcg gctgcggcgg gcgctcgcca acggcggccg ctggctcgac aagacgggca
     6721 aggcccgcct tgcggcggtg gtcgagaacc gcccgcgcct gaagcagatt gccgaatacc
     6781 gcgcgcgtct cgcggcattg atggagcagc gcggcaagcc ggaatcgacc ttggcggcat
     6841 tgcagcaatg gtgccgcgag gccgaagcca gcggcaaccg caagctgatc gagttcgcag
     6901 ctcgcctgaa acgctattcg gtcgcccggg cgtaatcggt gcgatcatcc gtgatcgctg
     6961 cgccacctac ccgagcttgc tgatacggca tcggtcctgg cgaagaccac cacggccttc
     7021 cctggccccc ggtcaaaatc acctcggggg caggctctga cttttcacgg cagccgctcc
     7081 gaacgtttca tctgcgtatg caaacccgcc gcaaggcggg tttttgctgc gcgcgggtgc
     7141 gaatgacaca atcctcgtcg atgacggccc tgtggcatcc tgcgatccgc gcggactggc
     7201 tggtgctgcg acgcaccggc gccgttgcat tcgcgcgact tatcggcagg cctgcgttat
     7261 tgctcgcgtg ctgcgcggcc atcgcctgcg gtgacttcta cggcggctac ggatggtgcc
     7321 tcgtggctgg tgcgctgctc gcgctgggcg gcagccatgc gatcgcgcac caacagttgc
     7381 tgggtgcggc agagcggtgg cgattcgggt ggtgtggcgc cttgcccgtc gcccggggca
     7441 cgacggcggg cactttgttg ctggtcgcga cggcggcctt gatggtatcg gtcgtgttcg
     7501 tcacggcgct gctgctgggc gtgtccatgc cggcaccgca tcggggcgac ctgccttatg
     7561 cccttgccgc aatcgatctc ggactcgtct tcggcaccgc cagcgcggca gtgcgcgtgt
     7621 tccgccgcgg tgcgatcgcg cgggcgcgac acgcggacgg aattcgggaa ccattgtttc
     7681 cgctgccgtg gttgaacgac ccgcgcttgc cgcatctgct cgactggcag cgtcgcgcgg
     7741 cgctggtgcg gtggcggcgt ggcggcagtt tcgcgatggt cggggtggcc ttggcggcgg
     7801 tgccacgcgg gccctccctt ccgatggtag tcgccctgat gctgttggtg ctctcgtggt
     7861 cgtggctggc agtcgtaatg cgcgccagcg ccgatgcatc cactgcggca atacgcctgc
     7921 tcggcgcgac accgctggat gtccggcatg cgttcctggc atcgttgcgc tatccggtga
     7981 tggccgcgtc gtgcgcggtc gttccgatgg ccgttggagc gacattgggc gggcgcagcg
     8041 tgatcgcgct ggcgtggctc gtttgcgcgg gcgcgatttc ggcgtggccg attcttcgaa
     8101 tccttcgcgc aacgcgatct ccggatgctt ccgcatgacg gctttgctcc gcgccgccga
     8161 tttgtgcgcc gcctacggcg acacacgcgt gctcaggggc gtcgatgtgc aactcgagca
     8221 cggcgaattc gttgcgctga tcggtcccaa cggctcgggc aagaccacgc tgctgcacgc
     8281 gcttgccggc ctggtgccct tgtccggggg cagcgttcgc atcggcggct acgatctggc
     8341 gaaagcgccg cgacgcgcca aagcggtctt cgggttggcg gtcgatccgg ctcgcctgcc
     8401 cgcgttgttg acgggccgcg agtgccttgc gctgtttgcg ggcgcacggg gattccctgc
     8461 cattcctcct gcgacgtttg cgctcgggga aaccctgtcg ctgacgccgg tactcgatcg
     8521 gcgaatcgat ggctattcgc tgggcatgcg gcagaagctc gccatcctgc tgggactgct
     8581 cggcgagccg ccgttgttgt tgctcgacga gcctttcaac ggcctcgatc cgcggagtgc
     8641 gctggcattc aagcggcacc tggcggcgtt ggtggcccgg ggcgataccg gcgtgctgct
     8701 cgcaacgcat tcgctcgaca ccgccgagcg tcacgcgacg cgggtcatcc tgctgatgga
     8761 tggacgcgtg cggcacgtct ggaatgccgc cgaactcctc gccttgcgcg aaaaccggga
     8821 gcactcgctc gaactggcaa tggccgaggt ctgcgggaca tgatgccgcc ccggcggttc
     8881 ccttgccgcg aatggcgcct cggacaagct gcaggagtac gatccctcgg catgatccgc
     8941 gttgcccgtt cgcgatgacg aagcgacgcg ccagcatcgc ggtgctgacg ctgtgcctct
     9001 gcgcaagcgt cctgcccgca tcggcgcaga ccaccaccaa gcttccccgc atcacggtga
     9061 ccggaacgcc gtacgtcgag caccatggcg gctacgtgat ctcggggaat ttcaaggtcg
     9121 atccgcgcat gccctacgtg gtgtttcccg cccgcgccct ggtcgaggac gacatcctca
     9181 gcgtgcatcc cgtccacttg aacgacgacg attacctggt gctccaggaa tgcgtggcgt
     9241 ccgattgcag tcgcgcgcac ctc
//
LOCUS       DASSNH010000278         5573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_542893, whole genome shotgun sequence.
ACCESSION   DASSNH010000278 DASSNH010000000
VERSION     DASSNH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542893"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFK00_10210"
     CDS             <1..991
                     /locus_tag="VFK00_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7593363.1"
                     /translation="GERGAELTRHLLAFARRQALEPRTVRPDELVDGMRNLLRRTLGE
                     NIELAVEHAADVAKVSVDPTQFESALLNLCINARDAMPGGGKLMIETGNVILDENYAE
                     QRVDVAPGDYVRVSISDTGTGMSAEDAARAFEPFFTTKSRGQGTGLGLSMVYGFVKQS
                     GGHVGIYSELGHGTVVTLYLRQAKGREEPVTPVERGDVAGHGERILLVEDDDLVRAHA
                     MHLLANLGYGVVAATNGPEALELLREGVACDLLFTDVIMPGGMTGPKLAEAAHELRPG
                     LPVLYTSGYTENAIVHHGRVDPGVNLLHKPYRKPELAAKLRAVLDDQSRKRET"
     gene            991..2166
                     /locus_tag="VFK00_10215"
     CDS             991..2166
                     /locus_tag="VFK00_10215"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HET7593364.1"
                     /translation="MEAERPRRLLILDDEPAVAQTVAFSAQRHGFAVRTSESSGAFFE
                     DVTSFRPSHIIIDLLMPGMDGVEVLRNLATKGCSANIIMMSGMGSKVLESAQRVGLER
                     GLRIIGILPKPFKPKELRTLLDAHGRARPEPPEAADSGALLEDIESALQDGQFVLHYQ
                     PKVHLATGRAIGVEALVRWQHPTFGLLPPDRFLPLIEQSGNMDVLTVCVIEAGLAWLA
                     ETDDGSARQLAINISAGSLTDVGFTDLLHERCARFDVDPARIILELTETTAPQRTAEL
                     LDILTRVRLKGFQLSIDDFGTGYSSLVQLAQLPFSEIKIDRSFVCGMLSSEEARKIVA
                     TTVTLGKELQLTTVAEGVESEAQAQALAMLQCDQAQGYFFARPMDGEAARAWLRGRN"
     gene            complement(2201..3199)
                     /locus_tag="VFK00_10220"
     CDS             complement(2201..3199)
                     /locus_tag="VFK00_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET7593365.1"
                     /translation="MRKPEDMTLDGRARRGDGDATRPRLPGMNLLPRPARRHLRRQVL
                     AVLSRDAPAPDYDAPLGDPGLFGPDSVTWKIHADFPSMMAGGLASLMLQALHPLALAG
                     VWDHSAFRTDTLGRLRNTTAFVGRTTYAPRAPAEAAIERVLAIHRTVRGTAPDGRAYS
                     ADDPHLLMWVHCAGCWCFLRAYETYCGARIPAAMQDRYLEEMARVAEALGARDAPRSM
                     PALASFFAEVRPELVFDARTRETLRVLGSIRLPIPFSGLSRDLFMGAAAALLPPWALD
                     LMGRSALRRLRDRTSSRALRLIAPSVRDAMAEGGLAWRACRRTGADYARLYRWDTD"
     gene            3448..4563
                     /locus_tag="VFK00_10225"
     CDS             3448..4563
                     /locus_tag="VFK00_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7593366.1"
                     /translation="MQGEDPADPPDDAIPGGLRIGAAFTWRVLVIAAAIAGVGLAARA
                     LSEIVIPFLIAVILAALLVPLSKFLQRHRWPKWLAILASMLLGAAVVVGLVLLVTERV
                     LTTLPRLQQQVSALGDSLNTLLARHPFGLTPEKINNYLSELMLWVQHNASHVAQRAAS
                     AGKHLVKVLQGIFIVIFATLFFLIDGAAIWAWVTRLFPRRARPRIDAAGQAGWHTLTE
                     FSRIQLVVAAIDAVAIGFGAWLLGVPLATPIAAIVFFGALVPIIGSIVAGAAAVIIAL
                     LFNGLTDALIMLGIVLLVNQLEGNVLHPLITGSVVRVHPLAVVLGVTAGAVVAGIAGA
                     FFAVPLIATANAMINAAARYPLGANDPDVPGRGPVDP"
     gene            complement(4623..5258)
                     /gene="azu"
                     /locus_tag="VFK00_10230"
     CDS             complement(4623..5258)
                     /gene="azu"
                     /locus_tag="VFK00_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HET7593367.1"
                     /translation="MMNLKMLAPVAACTLALAACGGNDNSASNQPAPGTTSPMPAATA
                     AAPMTAAPATTTGMAGMPATTATAPGGAMASTGKPAATVTNCSTEIEANDQIQYNVGS
                     IVVPSSCTDFKITLKHTGNLPVASMGHDVVITKQSDMEAVDKESASAGLDNGYIKADD
                     SRVIAHTKLIGGGETTSVSFPVSKIKDGGPYVFFCSFPGHSALMHGSISVQ"
BASE COUNT          914 a         1882 c         1878 g          899 t
ORIGIN      
        1 cggcgaacgc ggggcggagc tgacccgcca cctgctcgcc ttcgcgcgcc ggcaggcgct
       61 cgaaccgcgc accgtccgtc ccgacgaact ggtcgacggc atgcgcaacc tgctgcggcg
      121 caccctgggc gagaacatcg aactcgcggt ggagcacgcc gccgatgtcg ccaaggtttc
      181 ggtggatccc acccaattcg agtcggcgct gctgaacctg tgcatcaacg cgcgcgacgc
      241 gatgccgggc ggcggcaagc tgatgatcga aaccggcaac gtgatcctcg acgagaacta
      301 cgccgaacag cgcgtggacg tcgcgcccgg cgactatgtg cgggtttcga tctcggatac
      361 cggcaccggc atgagcgccg aagacgcggc gcgcgcgttc gaaccgtttt tcaccaccaa
      421 gtcgcgcgga cagggcaccg gtctcggcct cagcatggtg tacgggttcg tcaagcaatc
      481 cggcgggcac gtcgggatct actcagaact cgggcacggc accgtggtca cgctgtacct
      541 tcgccaggcc aaggggcggg aggagccggt aacgccggtg gagcgcggcg acgtcgcagg
      601 ccacggcgag cgcatcctgc tggtggagga cgacgacctg gtgcgcgcgc acgccatgca
      661 cctgcttgcg aacctcggct atggcgtggt ggcggccacc aacgggcccg aggcgctgga
      721 actgttgcgc gaaggcgtcg cctgcgacct gctgttcacc gacgtgatca tgcccggcgg
      781 catgaccggt cccaagcttg ccgaggccgc ccacgagctg cggcccggcc tgcccgtgct
      841 ctacacctcc ggctacaccg agaacgcgat cgtccaccac ggccgcgtcg acccgggcgt
      901 caacctgctg cacaagcctt accgcaagcc ggagctggcg gccaagctgc gcgcggttct
      961 ggacgaccaa tcaaggaaaa gggaaacctg atggaagcgg agcgaccgcg acgattgctg
     1021 attctcgacg acgagccggc ggtggcgcag accgtggcgt tctccgcgca gcggcatggc
     1081 ttcgccgtcc gcacgtcgga atcgagcggc gccttcttcg aagatgtcac gagcttccgg
     1141 cccagccaca tcatcatcga cctgctgatg cccggcatgg atggcgtgga ggtgctgcgc
     1201 aatctcgcga cgaagggatg cagcgccaac atcatcatga tgagcggcat gggcagcaag
     1261 gtgctggagt cggcgcagcg ggtcggcctc gagcgcggct tgcgcatcat cgggatcctg
     1321 cccaagccgt tcaaacccaa ggaactgcgg acgctgctcg atgcacacgg ccgcgcccga
     1381 cccgagccgc ccgaggctgc cgattccgga gcgcttctgg aagacatcga gtcggctttg
     1441 caggatggac agttcgtgct gcattaccag ccgaaggtcc acctcgccac tggccgcgcc
     1501 ataggcgtgg aggcgttggt gcgctggcag cacccgacgt tcgggctgct gccgcccgac
     1561 cggttcctgc cgttgatcga gcagtccggc aacatggacg tcctgaccgt ttgcgtcatc
     1621 gaggcaggcc tggcgtggct cgcggaaacc gacgatggca gtgcgcggca actggcgatc
     1681 aacatctccg ccggcagcct gaccgacgtg ggtttcaccg acctgctgca cgagcgatgt
     1741 gccaggttcg acgtcgaccc cgcccgtatc atcctggagc tgacggaaac gaccgccccg
     1801 cagcgcaccg ccgaactgct cgacatcctc acgcgcgtcc gcctcaaggg attccaactc
     1861 agcatcgacg acttcgggac cggctactcg tcgctggtgc aactggcgca gttgccgttt
     1921 tcggaaatca agatcgaccg atcgttcgtc tgcggcatgc tgtcttccga ggaggcacgc
     1981 aagatcgtcg ccaccaccgt gacgctcggc aaggaactgc aactcaccac cgtggccgag
     2041 ggcgtcgaaa gcgaagccca ggctcaggct ctcgcgatgc ttcaatgcga ccaggcacag
     2101 ggctatttct tcgcccgacc gatggatggc gaagctgcgc gggcgtggct gcgcgggcgc
     2161 aactagccgc ggccccagcg gctcgcgcac cgcgaacggg tcagtccgtg tcccaccggt
     2221 acaaacgcgc gtagtccgca ccggtgcgcc gacaggcgcg ccaggcgagg ccgccttcgg
     2281 ccatggcatc gcgcacgctc ggcgcgatca atcgcagcgc gcgcgaagac gtgcgatcac
     2341 gcaagcgccg gagggcggaa cgccccatca ggtccagtgc ccacggcggc agcagtgccg
     2401 cggcggcgcc catgaacaag tcgcggctca aacccgagaa gggaatcggc aagcggatcg
     2461 aaccgaggac ccgaagggtt tcgcgcgtgc gcgcatcgaa caccagctcc gggcgcacct
     2521 ccgcgaagaa cgaggcgagc gcgggcatcg atcgcggggc gtcgcgggcg ccgagtgctt
     2581 cggcgacgcg tgccatttcc tcgaggtaac gatcctgcat ggccgccggg atgcgcgccc
     2641 cgcaatacgt ctcgtacgcg cgcaggaaac accagcaccc ggcgcaatgc acccacatga
     2701 gcaggtgggg atcgtcggcg ctgtacgcac ggccatcggg cgctgtgccg cgcaccgtgc
     2761 ggtggatggc gagcacccgt tcgatggccg cctcggccgg cgcccgcggc gcatacgtcg
     2821 tgcggccgac gaacgccgtg gtgttgcgca ggcggccaag cgtgtcggtg cggaacgcgg
     2881 aatggtccca cacgcccgcc aacgccaacg gatgcagcgc ctgcagcatc agcgaagcca
     2941 ggccgccggc catcatcgac ggaaaatccg catggatttt ccaggtcacg ctgtcggggc
     3001 cgaacaagcc cggatcgccg agcggcgcgt cgtaatcggg tgccggcgca tcccggctca
     3061 ataccgccag cacctggcgt cgcaggtgcc gccgcgcggg ccgcggcagc aggttcattc
     3121 ccggaagacg cgggcgtgta gcatccccgt cgccgcggcg cgcacgtcca tcgagcgtca
     3181 tgtcttcggg tttgcgcatg caatcaactg tacaccgggg ccgtgaaatg ggccgcagca
     3241 catcggcggc gtgcacgagt accgcagact cgcggcagcc cgtgatacag gccttgacgc
     3301 caattcaacc cctgcgccat tagattccgg ggcgaccgtg tgcaatgcgg cgatgagtcc
     3361 tccgggcgga gtcgcgcgat ccatcccggc ggcggtcacg atcgcggcga cggcgtccgg
     3421 gacggacgca gggagatgct ggattccatg caaggcgaag atcccgcgga tccaccggac
     3481 gatgccattc cgggcgggct tcgcatcggc gcggcgttca cctggcgggt gctggtcatt
     3541 gccgcggcca tcgcgggggt cgggctggcc gcgcgcgcgt tgtccgaaat cgtcatcccg
     3601 ttcctgatcg cggtcatcct cgcggcactg ctggtgccgc tctcgaaatt cctgcaacgc
     3661 catcgctggc ccaaatggct ggcgatcctc gcctccatgc tgctcggcgc ggccgtggtc
     3721 gtcggcctgg tgctgctggt gaccgaacgc gtgctcacca ccctgccccg gctgcagcag
     3781 caggtcagcg ccctcggcga ttcgctgaac accctgctgg cgcggcatcc cttcggcctt
     3841 accccggaaa agatcaacaa ttacctgtcg gagctgatgc tatgggtgca acacaacgcc
     3901 tcgcacgtcg cccagcgtgc ggccagcgca ggcaagcacc tggtgaaggt gctgcagggc
     3961 atcttcatcg tcatcttcgc gaccttgttc ttcctgatcg acggtgccgc gatctgggca
     4021 tgggtgacgc ggctgttccc ccgccgggcc cgcccacgca tcgacgcggc cggccaggcg
     4081 ggctggcaca ccttgacgga gttttcccgg atccagttgg tggtggcggc gatcgatgcc
     4141 gtcgcgatcg gcttcggcgc gtggctgctc ggggtgccgc tggcgacccc gatcgccgcc
     4201 atcgtgttct tcggcgcgct ggtgcccatc atcgggtcca tcgtcgcggg cgccgccgcc
     4261 gtgatcatcg cgctgctgtt caacggcctg accgacgccc tgatcatgct cggcatcgtg
     4321 ctgctggtga accagctcga aggcaacgtc ctgcacccgc tgatcaccgg cagcgtcgtg
     4381 cgggtgcatc cactggcggt ggtgctcggc gtcaccgccg gcgcggtcgt cgcggggatc
     4441 gcaggcgcgt tcttcgccgt gcccctgatc gccaccgcga acgcgatgat caatgctgcc
     4501 gcgcgctatc cactcggcgc gaacgacccc gatgtacccg ggcgtggccc ggtcgatcct
     4561 tgaacccggc atggaacggg cgccgtcggg cgcccgttcc atgcgtcatt gcgaaagctg
     4621 cgttactgga cgctgatcga accgtgcatc agggcggaat gacccgggaa gctgcagaag
     4681 aacacgtaag ggccgccatc cttgatcttg ctcaccggga agctgaccga cgtggtctcg
     4741 ccgccgccga tcaacttggt gtgcgcgatc acgcgcgaat cgtccgcctt gatgtagcca
     4801 ttgtccaggc cggccgaggc gctttccttg tccaccgcct ccatgtccga ctgcttggtg
     4861 atgacaacgt catggcccat cgatgcgacc ggcaggttgc ccgtgtgctt caaggtgatc
     4921 ttgaaatccg tgcaggagga tggaacgaca atcgatccga cgttgtactg gatctggtcg
     4981 ttggcctcga tttcggtgct gcaattggtg acggtggccg cgggtttgcc ggtcgaggcc
     5041 atcgcaccac cgggtgccgt cgcggtggtg gccggcatgc cggccatgcc cgtcgtcgtt
     5101 gcgggcgcgg cggtcatcgg tgccgccgcc gttgcggcag gcatcgggct ggtggtgccc
     5161 ggcgccggtt ggttcgaagc ggaattgtcg ttgccgccgc aggccgcgag ggccagcgtg
     5221 caggcagcga cgggcgcaag catcttcagg ttcatcatcc agtctccttt gccaagacac
     5281 cggattatcg gcacgcaaag ctccacgtcg cgtgaagatg gaccgggcgc tccggatccc
     5341 ggccgttggc acggccgttg ccggcaattc gcatagaatc ggcacgcagc gtctgccgtc
     5401 gaggggtgaa tgcatgcacg gacagccaac ggctggcatg gtcgcggagt ggaatgcacg
     5461 cggcgttctc gtgctgccgc ggttctacgg ggatgccgag atcgacgccg cgctcgccga
     5521 ctacgtttcg ctctgggagg ccgggagcgc ccgcgtcacc gtggatgaca tgg
//
LOCUS       DASSNH010000279        18708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_129343, whole genome shotgun sequence.
ACCESSION   DASSNH010000279 DASSNH010000000
VERSION     DASSNH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 18708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18708
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129343"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..2276)
                     /locus_tag="VFK00_10235"
     CDS             complement(252..2276)
                     /locus_tag="VFK00_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7593368.1"
                     /translation="MAPSTDSTCAWNGNVSRRVRLATALLLGCIAWLAVGMARADATL
                     DPSLLPEIQSATFEVVAAKPKDTLTYEKPLPMDLLPYQERTDKYYSVGTAFAIGPNRF
                     VTAAHVMMIGYQSLWGPPELRDASGKVYAIDKIEKFALRRDFVVFSLVDPPKVTPLAI
                     DDKPSLNQVVFSVGNALGTGVVIRNGLYTSDTPEEQDGQWKWIRFSAAASPGNSGGPL
                     LDQQGKVIGVVLMKSPNENLNYALPMSEVRAAPEDLARYDKRMAYQFDAFDSTLTGTF
                     KGEFKLPLGLADFFATYGNAFHPWLDAQLKALLDQQSADLFPNGDGAHQLLFGGPSMN
                     NFPQLVVRNSDGVWVTGGHTTIRITLPSNGYAAGGLVKANVLFHLRKPDNIPDKQFHH
                     DPKGVMDMLLKTGFVKRRVGPDRILVTSLGEPDHTGTWTDRWGRHWQNWTWPVPYANG
                     FVDVFALPTPDGYSILMRVMPAATRHDTATNMQALTDFVSVPYSGTLAQWKAFLAESD
                     LLPEGFKKIRIDFDYDKDFRYASDRFEFSFTPKLQKIQPASMLTLGMTFFPDEDGKVV
                     WDVGQAWLAQDDHDHHWVSLLRRQAPPPDLADSYQSAWKKVVDRRHPYDGKAYNANDV
                     TRINAVVPPPNGDKASVLYTAYIDQPGQHPQAEMKKKLDLLLGKVQVKEP"
     gene            complement(2524..3342)
                     /gene="ccsA"
                     /locus_tag="VFK00_10240"
     CDS             complement(2524..3342)
                     /gene="ccsA"
                     /locus_tag="VFK00_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET7593369.1"
                     /translation="MPVHSTTMELLAVLSIALYLAAAAALARPLLDAGGAVPRRAALV
                     TGAIAVIIHFVVLFGIHRGAVDLHFYAALSLVSAVIAALTLVVNLVRPVAALGIILFP
                     IAGLFLAIDEFLAPVTAPFQLDWQIKLHVAIALVAYSLLAIAAVLALLLATQERALRS
                     RQFSAWARTLPPLTQTESLMFKLIGAGFALLTLTLLSGALFVHNLHAQHQAHTAILSL
                     VAWIIFGVLLIGRWRWGWRGPRAVNLTLLGMVILLLAFFGSKFVLEEILHRRGM"
     gene            3464..3751
                     /locus_tag="VFK00_10245"
     CDS             3464..3751
                     /locus_tag="VFK00_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HET7593370.1"
                     /translation="MKQQEVFRALADPTRRAILKRLQKGALSAGEIGAAFDITGASLS
                     HHFAMLKQADLVRTERRGRSIVYSLNTSVIEDLTRAVLDLVPTKRPRGDGR"
     gene            3748..4392
                     /locus_tag="VFK00_10250"
     CDS             3748..4392
                     /locus_tag="VFK00_10250"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HET7593371.1"
                     /translation="MKQTGTWMVSAAFVAAMLAIAVWIYPDLPPLAPTHWDAGGNVNG
                     WMPRFWAAALPVLVLLGLAILTPLLPCISPRKFEITPFANVYGVLMLVIQGVLLVIFT
                     CALLAGAGHHVPISLIAALSVGVLLMVIGNYMGKLRKNFFIGIRTPWTLASDVVWERT
                     HRFAGPLFVAAGAAWIGLSFVRAQPGWMFVPVVAAAVLPGVYSYVVYRRLEGHR"
     gene            4395..4931
                     /locus_tag="VFK00_10255"
     CDS             4395..4931
                     /locus_tag="VFK00_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7593372.1"
                     /translation="MELEAHPYFLRTERLGFRCWSPGDLELAMGLWGDPLVTRRIDSR
                     GRLPAAAVAERLQAEITMNEQHGIQYWPIFALATGEHVGCCGVRPYDPPARIHEFGVH
                     IRSACWRRGYAHEASRAVIDHAFDHLAARGLFAGHHPGNDASRRLLEKLGFRHTHDEF
                     YPPTGLMHPSYSLAPDAR"
     gene            4999..6366
                     /gene="ffh"
                     /locus_tag="VFK00_10260"
     CDS             4999..6366
                     /gene="ffh"
                     /locus_tag="VFK00_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7593373.1"
                     /translation="MFDNLTQRLSGTVQRLTGRGRLTEENIREALREVRVALLEADVA
                     LPVVQALIERIKVRAVGQEVLKSLTPGQALIKVVRDELTAVMGTQNAQLNLAATPPAV
                     VLMAGLQGAGKTTTVAKLAKHLIERRKKVMVVSADVYRPAAIEQLHTLADQVGAGFVP
                     SQTGQQPVAIVKGAIDAARREVADVLIVDTAGRTSIDEAMMAEIKALHAALDPVETLF
                     VVDSMTGQDAANTAKAFADALPLTGVILTKVDGDARGGAALSVRYVTGRPIKFLGAGE
                     KPDALEPFHPDRMATRILGMGDVLSLIEDVQQKTDADKAAKLAAKVAKGKRFDLNDMK
                     DQLDQMLNMGGLAGLMEKLPGVSGLPDKVKSQVNDKEVKKMMAIIDSMTKKERRHPDL
                     LKGSRRARVARGSGTQPADVNRLLKQYTQMSKMMSKFARGGMKGLMRQMGGAMKGMGG
                     RGFPG"
     gene            6681..6941
                     /gene="rpsP"
                     /locus_tag="VFK00_10265"
     CDS             6681..6941
                     /gene="rpsP"
                     /locus_tag="VFK00_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7593374.1"
                     /translation="MVRIRLSRAGARGRPFYHVVVTDQRSARDGRNIERLGYFNPGAA
                     GEHDQRLVLDVEKLNAWVAKGAQCSDKVAALAKQAAKQAQAA"
     gene            6946..7461
                     /gene="rimM"
                     /locus_tag="VFK00_10270"
     CDS             6946..7461
                     /gene="rimM"
                     /locus_tag="VFK00_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214776.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7593375.1"
                     /translation="MNDGRRVLLGRIVGVHGVRGMVKLESFTEPRTAIFDYRPWLLER
                     DAGGFETIAEAHGHAQGKGMVATLAGIGDRDAAATRIGARIWVPRDALPQPEPGEFYQ
                     TDLEGLEVVNEAGEDLGRVSHLFDNGAHGVLVTRDEAGRERLIPYVVDLYVKSVDMDA
                     GRITVDWDKQD"
     gene            7475..8236
                     /gene="trmD"
                     /locus_tag="VFK00_10275"
     CDS             7475..8236
                     /gene="trmD"
                     /locus_tag="VFK00_10275"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7593376.1"
                     /translation="MRFDVVTLFPGFVQQCSGFGVIGRAVERGLLEVAAWNPRDYAEG
                     GYRRVDDSVCGGGPGMVMLIEPLQKALAAARQAATDKAPVIYLSPQGKRLTQDKVRHL
                     ASLPRMILLCGRYEGIDERLLEHEVDEELSIGDYVLSGGELAAAVLIDAVGRLQEGAL
                     GDAQSAEQDSFSDGLLDCPHYTRPVTHALGDVPAVLLSGDHAAIRRWRRMQSLGRTWL
                     RRPDLLENVALDPDDQRLLEEFKREHKGNTHEQDH"
     gene            8220..8600
                     /gene="rplS"
                     /locus_tag="VFK00_10280"
     CDS             8220..8600
                     /gene="rplS"
                     /locus_tag="VFK00_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7593377.1"
                     /translation="MNKIIEQFEAAQMGRELPPFAAGDTVVVNVKVTEGNRERVQAFE
                     GVVIARRNRGLHSAFTVRKMSHGTGVERVFQAHSPAIDSIEVKRKGKVRGAKLYYLRG
                     LEGKAARIKEDIAARGKSDASRVK"
     gene            complement(9102..12224)
                     /locus_tag="VFK00_10285"
     CDS             complement(9102..12224)
                     /locus_tag="VFK00_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET7593378.1"
                     /translation="MPQFFIDRPIFAWVIAILITLGGTIAILNLPIEAYPTIAPPSVR
                     VRANYPGANAQVVQSTVTQVIEQNLSGIDHLLYFSSSSDSTGSSDITLYFDPGTDLDT
                     AAVQTQNAVNSAEPRLPQAVQNRGVTVRKSTGGHVMGISLQDKSGRLNPAALNNLIAA
                     QFVDPISRIEGVGSVDQFGSPYAMRIWLDPLKLKGYGLTASDALDAIRSQNVMFAAGS
                     VGAQPAMNGQGITATVTAESSFTTPEQFRQIILRTEPDGTTVTLGDVARVELGSESYG
                     NFSRLNGHPVASFGISLLPGANALAVSNAVLAKMKELAQNLPAGVSWSVPFESYTFVR
                     VSIEEVVKTLAEAIVLVFLVMLLFLQNLRATLIPTLVVPVALTGTFLGLYVLGFSINV
                     LSLFGMVLAIGIVVDDAIVVVENIERIMREEGLPAKEASRKGMQQITGAVIAVTTVLA
                     SVFVPAALMSGSVGAIYRQFSVTIAISMLLSALMALSFTPALCASILRPEHEHANRFL
                     RAFNNGYESMARHYLSRLGKAVRHTPRWMLGYGLAIVVAVVLLWNLPSSFVPQEDQGE
                     VVASIQLPPGATTGRTRQVMEEVERIVGNDPAVSNVFTIGGFSFSGHGENVGMAFIHL
                     KDWSDRSRTADDVINDFNAQFAKIRDAQVYAMNQAVIRGLGRFAGFDFELEDRGGLGH
                     RALMDAKDKLIAEAAKDKRLANVHVNGQPDSPTVDMQVDRVQAQSMGLSVTDVHNAIQ
                     LMLSQVYANDFYYGGRVLRVQVQADAPFRMNPSDIGQFYTRSSNGTMVPLSSVVDTKW
                     SLASPELDRYNGVGSISLTGTPAQGYSSGDAITAMQELVAKYLPTSFGYEWSGQSRQE
                     ILSGAQAPMLFGLSIFVVFLCLCALYESWSIPVAVMLVIPLGVLGSVALTSIRGLEDD
                     VYFKIGLIAIIGLSVKNAILIVQFAVDLQKQGKGLIVATLEACRIRLRPILMTSIAFI
                     LGVLPLAISTGAGANSRHAIGTGVIGGMIGATALGVMLVPLFYVVVRRLLGDKSDGTE
                     VERTKGEAHL"
     gene            complement(12394..13581)
                     /locus_tag="VFK00_10290"
     CDS             complement(12394..13581)
                     /locus_tag="VFK00_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7593379.1"
                     /translation="MTTWLLRGASATLLALALAACSHPAPPEPPPPEVGVVTARASDV
                     PVTRNYPGRLAATLTAQVRARVTGIVLERVYKEGSDVEAGDVLFKIDPAPLEATLRAA
                     QGQLAQAQATARNAEGKARRSEQLGAKGLLARQTIDDAVAAAAEAAAAVKSAQANVKN
                     AQINVGYATVTAPISGRAGRANVTQGALVSPSDTDPLTTVQVIDPIYANFSEPMAELE
                     KLRQAEKSGGLKLVAPDKAQVQIVLPDGSVYPHTGTLDFADRAVDPSTGAIDLRAVVP
                     NPDHVLLPGMFVNIRLKLGTLHDAFLVPQRAIQRDNDGAFVYVVAAGDKIAEKRVELG
                     DQHGSDWIVQGGLAAGEKVVVSGIQQASAGEKVKPVAEASKEGVNAASKAGNGKEAGN
                     GDG"
     gene            13772..>18708
                     /locus_tag="VFK00_10295"
     CDS             13772..>18708
                     /locus_tag="VFK00_10295"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET7593380.1"
                     /translation="MNATRAPAEDAEITAVSTEVRDRVGAVDAKAAEAFAKLVCAEVS
                     PDELARRGNETWAAMCIDLFAWFRTRPRGRARVRAFNPDRERNGWICQHSVIEVVTDD
                     MPFLVDSLEMAVAGAGLRLHRLLHPVMDVRRDADGNVDAISECRGGDSIESVMHAEID
                     RVDDATLQQLQTVAEVAFGDVRAAVADWQAMHDKMLAIADALPNETSPLGAEEIAEGQ
                     AFLRWAADNHFTFLGYREYDVVADQGDELLKARPGSGLGILRSPDLATAPRSTKSLVA
                     SRLPQSGSIDAIILTKTNARSRVHRPGYMDYIGVLRFDENGKPVGESRFLGLYTSTSY
                     MRRAQDVPLVRRKFESAEARSGLRPQSHSGKAFRTILETLPRDELLQASTDELFSLAS
                     GIFELSQRRRARLFVRRDAYGRFFSCLVFIPRDHYSPAVGDRVKEMLVEELHGAHADA
                     TVQIGDSPLARMHILVRPRTGEQVNVDMAKLEARLAPIVRSWHDELRDALVQGLGEER
                     GAQLANRWGRAFTAGYIEDTTPARAAEDVGILTAMTGAGDDMRLALHESYRTPGELHF
                     KIFRLGSDIPLSEALPLLENAGLKVDTEQVHVLDVRGSKACVQDFVVRPAAPLQFKLE
                     DLHARFEDAFHAIWHGRAENDHFNALVLRAGLGWRQIAMLRGYCKYQQQIGTPFSQAY
                     MEETLNRYPMIAGLLAELFESKFDPQRESGDAAVRKAAREALQRELQVLTPQTVRETN
                     PDFVDRVAGLLEGSRGTQCDGLVKAIKALLVNVTSLDDDRILRGFMDLMRATLRTNYF
                     QLHDGQPAEYISYKFDSAHVPDAPKPVPYREIWVYAPRVEGVHLRFGPVARGGIRWSD
                     RREDFRTEVLGLVKAQMVKNTVIVPVGSKGGFFVKRSPDASDRDAVLAEGIACYKMFI
                     NGLLDITDNIDTAAGKILHPADVVRHDDDDPYLVVAADKGTAKFSDIANGLSIEHGYW
                     LGDAFASGGSAGYDHKGMGITAKGGWESVKRHFRALGRDCQNEDFTCVGIGDMSGDVF
                     GNGMLLSRHTRLLAAFDHRHIFIDPDPAAEPSFVERERMFNLPRSSWDDYDKSKISKG
                     GGVFARSMKSIAITPEMRSALGIAEDVESLAPTELISAILKAPVDLLWNGGIGTYVKA
                     ASESNAEVGDRANNALRINGGDLRCSIVGEGGNLGFTQKGRIEAALAGVILNTDFIDN
                     SAGVDTSDHEVNIKILLNDAVQRGEMDTEARNALLHSMTDEVGRLVLRDNYRQNIALS
                     LMEHMSVTRIGSKAHFIRTLESRGQLDRQIESLPSDAELMERKMHHQGLTRPELAVLL
                     SYSKIVLYQQLLDSDVPEDPFLSHELVRYFPEPLHEKYAAHMQRHRLKREIIATAVTN
                     SIVNRMGATFVLRSQEDTGETAAAVVKAYNAARQILHARELWAGIESLDGKVSEASQI
                     DALMKVWSLLRHVSRWLLNRPGTVLGITDLVQRYQPGMDTLRAALPARLTETGRASWG
                     VDLEKWQGMGFPDELTHKLATIPSLEVAMDVIETSSESGRPVEHVAQVFFDLGAAMDI
                     EWLRGQIEKLPVESRWHAQARGALRDELAVQQRAMVSQILASEQGRQGADGAVAAWLQ
                     RDNPQMQFTRSMLDEIRGVDVDYPIASVALRRLAQ"
BASE COUNT         3197 a         6153 c         6324 g         3034 t
ORIGIN      
        1 cggtccccag ggcgttgggg tggaaaaggc cgaggctctt caacattgca gcaggccgga
       61 acacgtaatg gctccaggtc cacaggccac cggtgaggag cggccccagc acacgtcgct
      121 gcacgtcgaa tgcttcgctc atggtgcgca cgctccagga ggcgattgct gcgcctggat
      181 gatgctccta tgcgggccgc cggtccagtg acccgcgacc ggtcgcgccg agcgcccggc
      241 cgggtgccgg gctacggctc cttgacctgc accttgccca gcagcaggtc gagctttttc
      301 ttcatctcgg cttgcggatg ctggccgggc tggtcgatat aggcggtgta gagtaccgac
      361 gccttgtcgc cattcggcgg cggcaccacc gcgttgatcc tggtgacgtc gttggcgttg
      421 taggcctttc cgtcatacgg gtggcggcgg tccaccactt tcttccaggc gctctggtag
      481 ctgtcggcga ggtcgggggg cggtgcctgg cgacgcagca ggctgaccca gtgatggtcg
      541 tggtcatcct gcgcgagcca ggcctggccc acgtcccaga cgaccttgcc atcctcatcg
      601 gggaagaacg tcatcccgag ggtcagcatg ctggcgggct ggatcttctg cagcttcggc
      661 gtgaaggaaa attcgaaccg atccgatgcg tagcggaagt ccttgtcgta atcgaaatcg
      721 atccggattt tcttgaaacc ctccggcagc aggtcgcttt cggcgaggaa cgctttccat
      781 tgcgcgagcg tgccggagta gggcacgctg acgaagtcgg tgagcgcctg catgttggtc
      841 gcggtgtcgt gccgcgtcgc cgcgggcatt acccgcatca ggatgctgta gccgtccggc
      901 gtgggcagcg cgaacacgtc gacgaaaccg ttggcgtagg gcacgggcca cgtccagttc
      961 tgccagtggc ggccccagcg gtcggtccac gtgccggtgt ggtccggctc acccagcgag
     1021 gtcacgagga tccggtccgg tccgaccctg cgcttcacga agccggtctt cagcagcatg
     1081 tccatcacgc ccttggggtc gtggtggaat tgcttgtcgg gaatgttgtc gggcttgcgc
     1141 aggtggaaca gcacgttggc cttgaccagg ccgcccgcag catagccgtt cgaaggcagg
     1201 gtgatgcgga tggtggtgtg gccgccggtg acccacaccc cgtcgctgtt gcggacgacg
     1261 agctgcggga aattgttcat cgacggcccg ccgaacagga gctggtgcgc gccgtcgccg
     1321 ttcggaaaca ggtcggccga ctgctggtcg agcagcgcct tgagttgcgc atcgagccag
     1381 gggtggaacg cgttgccgta cgtcgcgaag aaatcggcga gccccagcgg cagcttgaat
     1441 tcgcccttga acgtccccgt gagggtggaa tcgaatgcgt cgaactggta ggccatgcgc
     1501 ttgtcgtagc gcgcgaggtc ctccggcgca gcccgcacct cgctcatcgg cagcgcgtag
     1561 ttgaggttct cgttgggcga cttcatcagc accacgccga ttaccttgcc ctgctggtcg
     1621 agcaagggac cgccgctgtt gccgggcgac gcggcggccg aaaaccggat ccacttccac
     1681 tggccgtcct gttcctccgg cgtgtcggag gtgtagaggc cgttgcggat cacgaccccg
     1741 gtgccgagcg cgttgccgac cgaaaacacc acctggttca acgaaggctt gtcgtcgatc
     1801 gccagcggcg tgaccttggg tggatcgacc agcgaaaaca cgacgaagtc gcggcgaagc
     1861 gcgaacttct cgatcttgtc gatcgcatac accttgcccg acgcatcgcg cagttcaggc
     1921 ggtccccaca ggctctggta gccgatcatc atgacgtgcg cggcggtgac gaagcggttg
     1981 gggccgatcg cgaaggcggt gccgaccgag tagtacttgt cggtgcgctc ctggtacggc
     2041 agcaggtcca tcggcagcgg tttttcgtag gtcagcgtgt ccttcggttt ggccgcgacc
     2101 acctcgaacg tcgccgactg gatctcgggc agcagcgacg ggtcgagggt ggcatcggcg
     2161 cgtgccatgc cgaccgcgag ccatgcgatg catccgagca gcaacgctgt cgcgaggcgc
     2221 acgcgccgcg acacgtttcc attccacgcg caggtggaat ccgtcgatgg ggccatggga
     2281 actccgtcag gacaagatca cgtcacaaac gccgcggatg gttgcacaac cgcagggtgg
     2341 ttgcacgccg agccatctgc gaggccgatg cgccgtcatt ccgggacaac ctggaggcaa
     2401 gcgcaaggtc caaaaatgga ttcccgatcg ccgctccgcg acgtcgagga tgacggcggg
     2461 atttgtcgcg cgttgacgca agcatcaatg gcggtatacg cgcctcgggt gccgcccgcc
     2521 gggctacatc ccgcggcggt gcaggatctc ctcaagcacg aacttcgagc cgaagaacgc
     2581 gagcagcaag atcaccatcc cgagcagcgt caggttgacc gcacgggggc cgcgccagcc
     2641 ccagcgccag cgcccgatga gcaacacgcc gaagatgatc cacgcgacca gcgacaggat
     2701 cgcggtgtgg gcctggtgct gggcgtgcag gttgtggacg aacagcgccc ccgacaggag
     2761 cgtcagcgtc aacagcgcaa aaccggcgcc gatcaacttg aacatcagcg attcggtttg
     2821 cgtaagcggt ggcagggtgc gcgcccaggc gctgaactgg cggctgcgca gcgcgcgttc
     2881 ctgggtcgcc agcagcagcg cgagcaccgc cgcgatcgcc agcaggctgt aggcgaccag
     2941 cgcgatcgca acgtggagct tgatctgcca gtcgagctgg aacggcgcag tcaccggcgc
     3001 gaggaattcg tcgatcgcga ggaacagccc ggcgatcggg aacaggatga tcccgagcgc
     3061 cgcgaccggc cgcacgaggt tcaccaccag cgtcaacgcc gcgatcaccg ccgagaccag
     3121 cgacaacgcg gcgtagaagt gcaggtccac tgcaccgcgg tggatgccga acagcaccac
     3181 gaagtggatg atcaccgcga tcgcgccggt caccagcgcc gcgcgccgcg gaaccgcgcc
     3241 gcccgcgtcc agcaacggac gggcgagcgc ggcggccgcc gcgagataca gggcgatcga
     3301 gaggaccgcc agcaattcca tcgtggtcga gtggacgggc attggtgcag tttcgcacac
     3361 ggcgcgaggc gcgcaccacc gtgaggccca gggcggtcga aggatacttg caatatttag
     3421 acgtttgtct aaatataagg gcaaggcccg cgaagtccga gtcatgaagc agcaggaggt
     3481 attccgtgcg ctcgccgatc ccactcgtcg cgcgatcctc aagcgcctgc agaagggcgc
     3541 gctcagcgcc ggcgagatcg gcgcggcgtt cgacatcacc ggcgcgtcgc tgtcgcacca
     3601 tttcgcgatg ctgaagcagg cggacctggt gcggacggaa cggcgcggtc ggagcatcgt
     3661 gtattcgctc aacacctcgg tcatcgaaga cctgacgagg gcggtcctgg acctggttcc
     3721 cacaaagcgg cctcgaggag acggcagatg aaacaaacgg gcacgtggat ggtttcggcg
     3781 gctttcgtcg ccgcgatgtt ggccattgcg gtgtggatct acccggacct gccgcccctg
     3841 gcgcccaccc attgggacgc gggcggcaac gtcaacggct ggatgccgcg cttctgggcg
     3901 gcggcgctcc cggtgctggt cttgctgggc ctagccatcc tgacgcccct gttgccctgc
     3961 atctcgccgc gcaaattcga aatcacgccg ttcgcgaacg tgtacggcgt actgatgctc
     4021 gtgatccagg gggtgctgct ggtgatcttc acctgcgcgc tgctcgccgg cgccggccac
     4081 cacgtgccga tatcgctgat cgcagcattg tccgtgggcg tactgttgat ggtaatcggc
     4141 aactacatgg gcaagctgcg caagaacttc ttcatcggaa tccgcacgcc atggacattg
     4201 gccagcgacg tggtgtggga acgtacccac cgctttgccg gacccttgtt cgtcgcggcc
     4261 ggggccgcgt ggatcggcct ctcgttcgtc cgggcacagc cgggctggat gttcgtgccc
     4321 gtcgtcgcgg ccgcggtgct tcccggtgtc tattcctacg tcgtctatcg gcgcctggaa
     4381 ggacaccgct gaacgtggag cttgaagcgc acccgtattt cctgcgcacc gagcggctcg
     4441 gtttcaggtg ctggagcccc ggcgatctcg agctggcgat ggggctctgg ggggatccgc
     4501 tcgtcacgcg gcggatcgac tccaggggcc ggttgcccgc cgctgcggtc gccgagcgcc
     4561 tgcaagccga aatcacgatg aacgagcagc atggcatcca gtactggccg atattcgcgc
     4621 tcgccaccgg tgaacacgtg ggctgctgcg gcgttcggcc ctacgacccg ccggcgcgca
     4681 tccacgagtt cggggtgcac atccgctccg cgtgctggcg ccgcggttac gcccacgaag
     4741 cgtcgcgcgc ggtgatcgac catgcgttcg atcatctcgc ggccaggggc ctgttcgccg
     4801 ggcaccatcc cgggaacgat gcctcccggc gtctgctgga gaagctcggg ttccggcata
     4861 cccacgacga gttctaccca cccaccggcc tgatgcaccc gtcctactcg ctcgcgcccg
     4921 acgcacgttg agcggcggcc gcatcgcctc gagcccggcc tccgctaaaa tgacggtttc
     4981 gctgttcgtg atcccgccat gttcgacaac ctgacccagc gcctttccgg caccgtccag
     5041 cgcctgaccg gccgcggccg gctgaccgag gaaaacatcc gcgaggcact gcgcgaagtg
     5101 cgcgtcgcgc tgctggaagc cgacgtcgcg ctgccggtgg tgcaggccct gatcgagcgc
     5161 atcaaggtgc gcgcggtcgg gcaggaagtc ctgaagtccc tgacgcccgg gcaggcgctg
     5221 atcaaggtcg tgcgcgacga gctgacggcg gtgatgggca cccagaacgc gcagctcaac
     5281 ctcgcggcga cgccgcccgc ggtggtgctg atggcgggcc tgcagggcgc cggcaagacc
     5341 accacggtcg ccaagctcgc gaaacacctg atcgagcgcc gcaagaaggt gatggtggtc
     5401 tcggccgacg tctatcgccc ggccgcgatc gagcagttgc acacgctcgc cgaccaggtc
     5461 ggcgccggct tcgtgccgtc gcaaaccggg cagcagccgg tggcgatcgt gaagggcgcg
     5521 atcgatgccg ccaggcgcga agtcgccgac gtgctgatcg tcgataccgc cggccgcacc
     5581 tcgatcgacg aggcgatgat ggccgagatc aaggcgctgc acgcagcgct cgatcccgtc
     5641 gaaaccctgt tcgtggtcga ttcgatgact ggccaggacg cggccaacac cgccaaggcg
     5701 tttgccgacg cgttgccgct gacgggcgtg atcctgacca aggtcgatgg cgacgcccga
     5761 ggcggcgcgg cgctctcggt gcgctacgtc accgggcggc cgatcaagtt cctgggtgcg
     5821 ggcgagaagc cggacgcgct ggaaccgttc catccggacc gcatggccac gcgcatcctc
     5881 ggcatgggcg acgtgctgtc gctgatcgag gacgtgcagc agaagaccga tgccgacaag
     5941 gccgccaagc tcgcggcgaa agtcgccaag ggcaagcgct tcgacctgaa cgacatgaag
     6001 gaccaactcg accagatgct caacatgggc ggccttgccg ggttgatgga aaaattgccc
     6061 ggcgtctcgg gcctgcccga caaggtcaag tcgcaggtca acgacaagga agtgaagaag
     6121 atgatggcga tcatcgattc gatgaccaag aaggagcggc gccatcccga tctcctcaag
     6181 ggttcgcggc gcgcacgcgt ggcgcgcggc tccggcaccc agccggccga cgtcaaccgg
     6241 ctgctgaagc agtacaccca gatgtccaag atgatgtcga agttcgcccg cggcggcatg
     6301 aagggcctga tgcggcaaat gggcggcgcc atgaagggca tgggcgggcg cgggtttccc
     6361 ggctgaagag cgtaggttgg gttgatcccg gaccttgtcc gggagaagcc caacgttgcg
     6421 aagcggtttg tccgaattca tgtcattcgg agtcgggtgt gaagtgtgct tggccgaacc
     6481 ttgccgatgc gggtccgggc cgggtatccc aagtccgaag ggtgcttgac gttgggcttc
     6541 tcccgggcag agcccgggat caacccaacc taccggaaaa ccctcgcatt tcgccggttt
     6601 tgcccgtaca attacccgtt ttcgcgcctc ggggtcgccc gcgaccgtcc gtggccgttt
     6661 tcattcggag tcgacagatc atggtcagga ttcgtctttc gcgcgcaggc gccaggggcc
     6721 ggccgttcta ccacgtcgtg gtgaccgacc agcgcagcgc ccgcgacgga cgcaacatcg
     6781 aacgcctggg ttacttcaac cccggcgcgg caggtgagca cgaccagcgc ctggtgctgg
     6841 acgtggagaa gctgaatgcc tgggtcgcca agggcgccca gtgctcggac aaggtcgctg
     6901 cgctggccaa gcaagcggcg aagcaggcgc aagccgcctg acgcgatgaa cgacgggcgg
     6961 cgggtgctgc tcgggcggat cgtcggtgtg catggcgtcc gcggcatggt gaagctggaa
     7021 tcgttcaccg aaccgcgaac ggcgattttc gattacaggc cctggttgct ggagcgcgac
     7081 gcgggcggat tcgaaaccat cgccgaggcg catggacacg cgcagggcaa gggcatggtg
     7141 gcgacgctgg cgggaatcgg cgatcgcgat gccgcggcaa cgcggatcgg cgcgcgcatc
     7201 tgggttccac gcgacgcgct gccgcaaccg gagcctggcg agttctacca gacggacctg
     7261 gaaggattgg aggtggtgaa cgaggcgggc gaggacctgg gtcgcgtcag ccacttgttc
     7321 gacaacggcg cgcacggcgt gctggtgacg cgcgacgaag ccggacgcga gcgcctgatc
     7381 ccgtacgtcg tggacctgta cgtgaaatcg gtcgacatgg atgcgggacg catcacggtc
     7441 gactgggaca aacaagattg accagggacg accgatgcgc ttcgacgtcg tcacgctgtt
     7501 ccccgggttc gtgcagcagt gcagcggttt cggggtgatc gggcgggccg tcgagcgcgg
     7561 cttgctggaa gtggcggcct ggaacccgcg cgattacgcg gaaggcggtt accgccgggt
     7621 cgacgacagc gtgtgcggtg gcggtcccgg catggtgatg ctgatcgagc cgctgcagaa
     7681 ggcgctggcg gcggcccggc aggcggcgac ggacaaggcg ccggtgattt acctgagtcc
     7741 gcagggaaag cggctcacgc aggacaaggt gcggcacctg gcgtcactgc cgcggatgat
     7801 tttgctgtgc gggcgctacg aaggaatcga cgagcgcctg ctggaacacg aggtcgacga
     7861 ggaactgtcg atcggcgatt acgtgctgtc gggcggcgaa ctggctgcgg cggtgctgat
     7921 cgacgcggtc ggcaggttgc aggaaggtgc gctgggcgat gcgcaatcgg cggagcagga
     7981 ttcgttttcg gacggtctgc tggattgccc gcactacacg cggccggtga cccacgcgtt
     8041 gggcgacgtg ccggcggtgt tgttgtcggg cgaccacgcg gcgatccgcc gctggcgccg
     8101 gatgcagtcg ctgggccgca cctggttgcg tcggccggac ctgctggaaa acgtggcgct
     8161 ggacccagac gaccaacgcc tgctggaaga attcaaacgg gaacacaagg gcaacaccca
     8221 tgaacaagat cattgaacaa ttcgaagccg cgcagatggg ccgtgagctg ccgccattcg
     8281 ccgccggcga caccgtggtc gtcaacgtca aggtcaccga aggcaaccgc gagcgcgtgc
     8341 aggcgttcga gggcgtggtg atcgcgcgcc gcaaccgcgg cctgcattcg gccttcaccg
     8401 tgcgcaagat gtcgcacggc accggcgtcg agcgcgtgtt ccaggcgcac agccccgcga
     8461 tcgactcgat cgaagtcaag cgcaagggca aggtgcgcgg cgccaagctg tattacctgc
     8521 gcggcctgga aggcaaggcc gcccgcatca aggaagacat cgccgcacgg ggcaagtccg
     8581 acgcttcgcg cgtgaagtaa tccggcaact acacgtttca agacgaagcc gcgcggagcg
     8641 atccgcgcgg cttttgtttg ccgggggcaa gcgtgacccg agccctgctt ttcgttctcc
     8701 ctccgggaga aggtggcgcg aagcgccggg cggggttcgc tgtcacatgc gcctccatcg
     8761 ccgctggccc gccccgaggc atgaaaacct cgccgcgtgc gcgcatcgtg gagccttggt
     8821 ttcgcgaggt gcatgcgcgg tcatgcgccg gtgttgggct tcgcggatga agcccgctga
     8881 acccaacctg caaatgctgc aggagcgcgc catgcgcgcg atggttgtgg atcaaccgcg
     8941 gcggcgatcg cggccatggg ccgctcctac gacggttttt tgtaggttgg gttgacgccg
     9001 gattcatccg gcggaagccc aacattccgc catcctaccc cggtcgcgga acgcccctgc
     9061 gtttgcgtcg ggcttcgcgg atgaagtccg ctcaacccaa cctacaaatg cgcttcaccc
     9121 ttggtgcgct cgacttcggt gccgtcgctc ttgtcgccga gcaagcgccg caccacgacg
     9181 tagaacaacg ggaccagcat cacgccgagg gcggtggcgc cgatcatgcc gccgatcacc
     9241 ccggttccga tggcgtggcg gctgttcgcg ccagcgccgg tcgaaatcgc cagcggcagc
     9301 acgcccagga tgaaggcgat cgacgtcatc aggatcggac gcaggcggat gcggcaggct
     9361 tccagcgtcg ccacgatcag gcccttgccc tgtttctgca ggtccaccgc gaactgcacg
     9421 atcaggatcg cgttcttcac cgacaagccg atgatcgcga tcagcccgat cttgaagtac
     9481 acgtcgtcct ccagcccgcg gatgctggtg agcgcaaccg atcccagcac gcccagcggg
     9541 atcaccagca tcaccgcgac cgggatcgac cagctttcgt acagtgcgca caggcacagg
     9601 aataccacga agatcgacag cccgaaaagc atcggcgcct gcgcgccgga caggatctcc
     9661 tggcgcgact ggccggacca ctcgtaaccg aagctcgtcg gcaggtactt ggcgaccagt
     9721 tcctgcatcg cggtgatcgc gtcgcccgag ctgtaacctt gcgccggggt gccggtgagc
     9781 gagatcgagc cgacgccgtt gtagcggtct agttccggcg aggccagcga ccacttggta
     9841 tcgaccacgc tcgacaacgg caccatcgtg ccgttggacg agcgcgtgta gaactggccg
     9901 atgtcggacg ggttcatccg gaatggcgca tccgcctgca cttgcacgcg caacacgcgg
     9961 ccgccgtagt agaagtcgtt ggcgtacacc tgcgacagca tcaactggat cgcgttgtgc
    10021 acgtcggtga cggacagccc catcgactgc gcctgcacgc ggtcgacctg catgtccacg
    10081 gtcggtgaat ccggctggcc attgacgtgg acgttggcga gccgcttgtc cttggcggct
    10141 tcagcgatca gcttgtcctt ggcgtccatc agcgcgcggt ggcccaagcc gccacggtct
    10201 tccagctcga aatcgaaacc ggcgaagcgt cccaggccgc ggatcaccgc ctggttcatc
    10261 gcgtacacct gggcatcgcg gatcttcgcg aactgcgcgt tgaagtcgtt gatgacgtcg
    10321 tccgcggtgc gcgaacggtc gctccagtcc ttcaggtgga tgaaggccat gccgacgttt
    10381 tcgccgtggc cggaaaagct gaagccgccg atcgtgaaca cgttggagac ggcgggatcg
    10441 ttccccacga tccgctcgac ctcttccatc acctggcggg tgcgcccggt ggttgcgccc
    10501 ggcggcaact ggatgctggc gacgacttcg ccctggtctt cctgcggcac gaagctcgag
    10561 ggcaggttcc agagcagcac gaccgccacc acgatcgcga gcccgtagcc cagcatccag
    10621 cgcggcgtgt gccgcaccgc cttgccgagc cgcgacaggt aatgccgcgc catgctttcg
    10681 taaccgttgt tgaaggcgcg caggaaacgg ttggcgtgtt cgtgctcggg ccgcaggatg
    10741 ctcgcgcaca aggccggcgt gaacgacagc gccatcagcg ccgacagcag catcgagatc
    10801 gcgatcgtca ccgagaactg gcggtagatc gcgccgacgc tgcccgacat cagcgccgcc
    10861 ggcacgaaca ccgaggcgag cacggtggtc accgcgatca ccgcgccggt gatctgctgc
    10921 atgcccttgc ggctggcttc cttcgcgggc aatccctctt cgcgcatgat gcgctcgatg
    10981 ttttccacca ccacgatcgc gtcgtcgacc acgatgccga tcgccagcac catcccgaac
    11041 aggctcagca cgttgatcga gaatccgagc acgtagaggc cgaggaacgt accggtcagc
    11101 gccaccggca ccaccagggt gggaatcagc gtggcgcgca ggttctgcag gaacagcagc
    11161 atcaccagga acaccagcac gatcgcctcg gcgagggtct tcaccacctc ctcgatcgaa
    11221 acccggacga aggtgtagct ctcgaacggc acgctccagc tgacgcccgc cggaaggttc
    11281 tgggccagct ccttcatctt cgccagcacc gcgttggaaa cggccagcgc gttggccccc
    11341 ggcagcaacg aaatgccgaa cgacgccacc gggtgcccgt tgaggcgcga gaaattcccg
    11401 tagctttccg aaccgagctc gacccgcgcg acgtcgccga gggtcaccgt ggtgccgtcg
    11461 ggttcggtgc gcaggatgat ctggcggaac tgctcgggcg tggtgaaact cgattccgcc
    11521 gtgaccgtgg cggtaatccc ctggccgttc atggctggct gcgcgcccac cgaacccgcg
    11581 gcgaacatca cgttctgcga ccgtatcgcg tccagcgcgt cgctggcggt gaggccgtaa
    11641 cccttcagct tcagcgggtc cagccagatc cgcatggcgt acggcgagcc gaactggtcg
    11701 acgctgccga cgccttcgat gcgcgagatc ggatcgacga attgcgcggc gatcaggttg
    11761 ttgagcgcgg cgggattgag gcgtccgctc ttgtcctgca gcgaaatgcc catgacgtgc
    11821 ccgccggtgg acttgcgcac cgtgacgccg cggttctgca cggcctgcgg caggcgcggt
    11881 tcggccgagt tcacggcgtt ctgggtctgc accgcggccg tatccaggtc cgtgcccgga
    11941 tcgaaataca gcgtgatgtc ggacgagccg gtcgaatccg acgacgagct gaaatacagc
    12001 aggtggtcga tgccggagag gttctgctcg atcacctgcg tgaccgtgct ctgcaccacc
    12061 tgcgcgttgg cgcccggata attcgcccgc acccgcacgc tcggcggcgc aatggtggga
    12121 taggcctcga tcggcaggtt gaggatcgcg atggttccgc ccagcgtgat caggatcgcg
    12181 atcacccacg cgaagatcgg acggtcgatg aagaattggg gcatggccta gaagcgtccg
    12241 gatttgactg ccgcgtgcgc cgatccggcg gtggagcagg gggatgggag atgggcggtg
    12301 ggggcgccgt cccaagggcg tcggagcgga ttgacgactg gcgcgcaaac gcaagtctcg
    12361 ggactcgcgc cagggtattt cggagcgccc ccgctaccca tccccattcc ccgcttcttt
    12421 cccgttgccc gctttgctgg ctgcgttgac gccttccttc gacgcctcgg ccaccggttt
    12481 caccttctcg cccgcgctcg cctgctggat gcccgagacc accaccttct cgccggcggc
    12541 gaggccgccc tgcacgatcc agtcggagcc gtgctggtcc ccgagctcga cgcgcttttc
    12601 cgcgatcttg tcgcccgccg cgaccacgta cacgaaggca ccgtcgttgt cgcgctggat
    12661 cgcgcgctgc gggaccagga acgcgtcgtg cagcgtgccg agcttcaagc ggatgttcac
    12721 gaacatgccc ggcaacagca cgtggtcggg gttcgggacc accgcccgca ggtcgatcgc
    12781 gccggtgctc gggtccacgg cgcggtccgc gaaatcgagc gtgccggtgt gcgggtacac
    12841 gctgccgtcg ggcagcacga tctgcacctg cgccttgtcg ggcgcgacca gtttcaggcc
    12901 gccggacttc tccgcctgcc ggagtttctc gagttccgcc atcggctcgc tgaagttggc
    12961 atagatcggg tcgatcacct gcaccgtggt cagcggatcg gtgtcgctgg gcgagaccag
    13021 ggcaccctgg gtcacgttgg cgcgcccggc gcggccgctg atcggtgcgg tgacggtggc
    13081 gtagccgacg ttgatctggg cgttcttgac gttggcctgt gccgatttga ccgcggcggc
    13141 ggcctccgcg gcggcggcca ccgcgtcgtc gatggtctgc ctggcgagca ggcccttggc
    13201 gccgagctgc tcgctgcgcc tcgccttgcc ctcggcgttg cgggcggtgg cctgcgcctg
    13261 cgccaattgg ccttgcgccg cgcgcagcgt ggcttcgagc ggcgccggat cgatcttgaa
    13321 cagcacgtcg ccggcttcga cgtcgctgcc ttccttgtag acgcgttcca gcacgatgcc
    13381 ggtgacgcgc gcgcgcacct gcgccgtcag ggtggcggcg aggcgacccg gatagttgcg
    13441 ggttacgggt acgtcgcttg cgcgcgccgt caccaccccg acttcgggtg gcggcggctc
    13501 cggcggagcc ggatggctgc acgcagcgag cgccagtgcc agcaaagttg cgctcgcacc
    13561 gcgcagcagc caggtcgtca tggggttctc ggtcgacacg gataacaaac gcgtcattat
    13621 cgcgtaatcc gccccacgcg tccctaggcc aagggtcacg gaaggccgct gccgaaacgg
    13681 tccgaaagcg gccccgggag ggcgccaagc tggaacgcca aggccacgcg gcggtatcct
    13741 acgcgttccc atcggccctt caagaatcga catgaacgca acgcgtgcgc ccgcagagga
    13801 tgctgaaatc accgcagtct cgaccgaggt ccgggaccgg gtgggggcgg tcgacgcgaa
    13861 ggcggcggaa gcgttcgcga aactggtgtg cgcggaggtg agccccgacg aactcgcgcg
    13921 ccgcggcaac gagacctggg ccgcgatgtg catcgatctt ttcgcgtggt tccgcactcg
    13981 tccgcgcggc cgcgcgcggg tgcgcgcgtt caatcccgac cgcgagcgca acggctggat
    14041 ctgtcagcac tcggtcattg aagtcgtcac cgacgacatg ccgttcctgg tcgattcgct
    14101 ggagatggcg gtggccggcg cgggcctgcg cctgcaccgc ctgctgcacc cggtgatgga
    14161 cgttcgccgc gacgcggacg gcaacgtcga cgcgatttcg gagtgccgtg gcggcgattc
    14221 gatcgagtcg gtgatgcacg ccgagatcga ccgcgtggac gacgccaccc ttcagcaact
    14281 gcagacggtg gccgaggtgg cgttcggcga cgtccgcgcc gccgtcgccg actggcaggc
    14341 catgcacgac aagatgctgg cgatcgcgga cgcgctgccg aacgaaacga gtcccctggg
    14401 cgccgaggaa atcgccgaag gccaggcgtt cctgcgctgg gccgcggaca accacttcac
    14461 cttcctcggc tatcgcgaat acgatgtggt cgccgaccag ggcgacgaat tgctgaaggc
    14521 ccggccgggc tcgggcctcg ggatcctgcg cagtccggat ctcgccaccg cgccgcgttc
    14581 gaccaagagc ctggtcgcga gccggctgcc gcagtcgggt tcgatcgacg cgatcatcct
    14641 gaccaagacc aatgcgcgct cgcgtgtaca ccggccaggc tacatggatt acatcggcgt
    14701 gctgcgcttc gacgagaacg gcaagccggt cggcgagtcg cggttcctcg gcctctacac
    14761 ctccacctcg tacatgcgcc gcgcccagga cgtgccgctg gtgcgcagga agttcgaatc
    14821 cgccgaggcg cgttccggcc tgcggccgca atcccattcg ggcaaggcgt tccggacgat
    14881 cctggaaacc ctgccgcgcg acgaactgct gcaggcctcc accgacgaat tgttctcgct
    14941 cgcgtcgggc atcttcgagt tgagtcagcg ccgccgcgcg cgcctgttcg tgcgccgcga
    15001 cgcctacggc cgcttcttct cgtgcctggt gttcatcccg cgcgaccact acagccccgc
    15061 cgtcggcgac cgcgtcaagg agatgctggt cgaggaactg catggcgcgc acgccgacgc
    15121 cacggtgcag atcggcgact cgccgctcgc ccgaatgcac atcctggtgc gcccgcgcac
    15181 cggcgagcag gtgaacgtcg acatggccaa gctcgaggcg cggctcgccc cgatcgtgcg
    15241 gagctggcac gacgaactgc gcgatgcgct ggtgcagggg ctcggcgaag aacgcggcgc
    15301 gcaactcgcc aaccgctggg gccgcgcctt cacggcgggc tacatcgagg acaccacgcc
    15361 ggcgcgcgcg gcggaggacg tcggcatcct gaccgcgatg accggcgccg gcgacgacat
    15421 gcgcctcgcg ctgcacgagt cctaccgcac gcccggcgaa ctgcacttca agatcttccg
    15481 cctgggcagc gacatcccgt tgtccgaggc gctgcccctg ctcgagaacg ccgggctcaa
    15541 ggtggacacc gagcaggtgc acgtgctgga cgtgcgcggc agcaaagcct gcgtgcagga
    15601 tttcgtggtg cgcccggcag cgccgctgca gttcaagctc gaggacctgc atgcgcgctt
    15661 cgaggatgcc ttccacgcga tctggcacgg ccgcgcggag aacgaccatt tcaatgcgct
    15721 ggtgctgcgc gccgggctgg gctggcgcca gatcgcgatg ctgcgcggct actgcaaata
    15781 ccagcagcag atcgggacgc cgttttcgca ggcgtacatg gaagagacgc tcaaccgcta
    15841 tccgatgatc gcggggttgc tcgccgaatt gttcgaatcc aagttcgacc cgcagcgcga
    15901 atccggcgat gccgccgtgc gcaaggccgc ccgcgaagcc ctgcaacgcg agctgcaggt
    15961 gctgacaccg cagacggtgc gcgaaacgaa tccggatttc gtcgaccgcg tggccgggtt
    16021 gctggaaggt tcgcgcggca cccagtgcga tggcctggtc aaggcgatca aggccttgct
    16081 ggtcaacgtc accagcctcg acgacgaccg catcctgcgc ggtttcatgg acctcatgcg
    16141 ggccacgctg cgcaccaact acttccagtt gcacgacggc cagccggccg aatacatcag
    16201 ctacaagttc gattcggcgc acgtgcccga tgcgcccaag cccgtgccgt accgcgaaat
    16261 ctgggtgtac gcgccgcgcg tcgaaggcgt gcacctgcgc ttcgggccgg tggcgcgcgg
    16321 cggcatccgc tggtcggacc gacgcgagga cttccgcacc gaggtgctgg gcctcgtcaa
    16381 ggcgcagatg gtgaagaaca ccgtgatcgt gccggtgggc tccaagggcg gattcttcgt
    16441 gaagcgctcg cccgatgcga gcgaccgcga cgcagtcctg gcggagggca tcgcctgcta
    16501 caagatgttc atcaacggcc tgctggacat caccgacaac atcgacaccg cggccggcaa
    16561 gatcctgcat ccggccgacg tggtccgcca cgacgacgac gacccgtacc tggtggtcgc
    16621 cgccgacaag ggcaccgcga agttctccga catcgccaac ggcctgtcca tcgagcacgg
    16681 ctactggctg ggcgatgcat tcgcgtccgg cggctcggcc ggctacgacc acaagggcat
    16741 gggcatcacc gccaagggcg gctgggaatc ggtcaaacgg cacttccgcg cactgggccg
    16801 cgactgccag aatgaggact tcacctgcgt cggcatcggc gacatgtccg gcgacgtgtt
    16861 cggcaacggc atgctgctgt cgcgccacac ccggctcctg gccgcgttcg accaccgcca
    16921 catcttcatc gatccggatc cggccgccga gccgtcgttc gtcgaacgcg agcggatgtt
    16981 caacctgccg cgctcgtcgt gggacgacta cgacaagtcg aagatctcca agggcggcgg
    17041 cgtgttcgcg cgcagcatga agagcatcgc gatcaccccg gagatgcgca gcgcgctcgg
    17101 catcgccgag gacgtcgagt cgctggcgcc gaccgaactc atcagcgcca tcctcaaggc
    17161 gccggtcgac ctgctctgga acggcggcat cggcacctac gtgaaagccg cctcggaatc
    17221 caatgccgag gtcggtgacc gcgccaacaa cgcgctgcgc atcaacggcg gcgacctgcg
    17281 ctgcagcatc gtgggcgagg gcggcaacct cgggttcacc cagaagggtc gcatcgaggc
    17341 cgcgctggcg ggcgtgatcc tcaacaccga cttcatcgac aactcggcgg gcgtcgacac
    17401 ctccgaccac gaggtcaaca tcaagatcct gctgaacgat gccgtgcagc gcggcgagat
    17461 ggacaccgaa gcgcgcaacg cattgctgca ttcgatgacc gacgaggtcg ggcggctggt
    17521 gctgcgcgac aactaccggc agaacatcgc gctcagcctg atggagcaca tgtcggtgac
    17581 ccgcatcggc tccaaggcgc acttcatccg cacgctcgaa tcgcgcgggc agctcgatcg
    17641 ccagatcgaa tccttgcctt ccgacgcgga actgatggag cgcaagatgc accaccaggg
    17701 cctgacgcgg ccggaactcg cggtactgct gtcgtactcc aagatcgtgc tgtaccagca
    17761 attgctcgac tccgatgttc ccgaggatcc gttcctgtcc cacgagctgg tgcgctactt
    17821 cccggagccg ctgcacgaga aatacgccgc gcacatgcag cggcaccgcc tgaagcgaga
    17881 gatcatcgcc accgcggtca ccaattcgat cgtcaaccgg atgggcgcca ccttcgtgct
    17941 gcgcagccag gaagacaccg gcgagaccgc cgccgcggtg gtgaaggcct acaacgccgc
    18001 gcgccagatc ctgcacgcac gcgaactgtg ggccggcatc gagtcgctgg acggcaaggt
    18061 cagcgaggct tcgcagatcg acgcgctgat gaaggtgtgg tcgctgctgc gccacgtttc
    18121 gcgctggctg ctcaaccgcc cggggacggt gctgggcatc accgacctgg tgcagcgcta
    18181 ccagcccggc atggacacgc tacgtgcggc gctgcccgcg aggctcacgg aaaccggccg
    18241 cgcgtcgtgg ggcgtagacc tcgagaagtg gcagggcatg ggtttccccg acgaactcac
    18301 ccacaagctg gccacgatcc cctcgctcga agtcgcgatg gacgtgatcg agacgtcctc
    18361 ggaatccggt cgcccggtcg agcacgtcgc gcaggtgttc ttcgatctcg gcgcggcgat
    18421 ggacatcgaa tggctgcgcg gccagatcga gaaactgccg gtcgaaagcc gctggcacgc
    18481 ccaggcgcgc ggcgccctgc gcgacgaact cgcggtgcag cagcgcgcga tggtcagcca
    18541 gatcctcgcg tccgagcagg gcaggcaggg cgccgacggc gcggtggccg catggctgca
    18601 gcgcgacaac ccgcagatgc aattcacccg cagcatgctc gacgaaatcc gcggcgtcga
    18661 cgtcgactac ccgatcgcct ccgtcgcact ccgccggctc gcgcaaat
//
LOCUS       DASSNH010000280         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_39796, whole genome shotgun sequence.
ACCESSION   DASSNH010000280 DASSNH010000000
VERSION     DASSNH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39796"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..919
                     /gene="motA"
                     /locus_tag="VFK00_10300"
     CDS             50..919
                     /gene="motA"
                     /locus_tag="VFK00_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HET7593381.1"
                     /translation="MLVLVGTLVVIVCVMGGYVMSHGHLLALWQPYELMIIFGGAAGA
                     FLTSNPGKVVKSALREVAGLAKGPTYKRQDYVDLLSLLYDIFAVMRKQGVLALEPHIE
                     DPQSSSVFAAYPRLLREHYLVEFITDCLRLIVGGNMEPHELEALLDAEVENHHAEAEA
                     PAQAVAKMADALPGFGIVAAVLGIVTTMSNLTGDTSQIGEHIAGALVGTFLGILLCYG
                     FIGPLAAAMEARVREDGKAFECVKVALLASLRGYNPKVAVEFARKALSAHSRPNFQDL
                     EEHLKGARAGAPA"
     gene            916..1785
                     /gene="motB"
                     /locus_tag="VFK00_10305"
                     /pseudo
     CDS             916..1785
                     /gene="motB"
                     /locus_tag="VFK00_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
     gene            complement(2043..3209)
                     /locus_tag="VFK00_10310"
     CDS             complement(2043..3209)
                     /locus_tag="VFK00_10310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7593382.1"
                     /translation="MTRSTQFQHDCVVVGASFAGLACAHALAGAGLRVAVYEQKGDPG
                     EKLHTTGIIVKDAIDQIALLDDLPAQLVRRISGVRLYAPNLRHVDLAAPGYYFLATDT
                     PNVMRWLAGRAEEAGARLHCGQVFREAHRQRSGFDLGELGQTRFLVGADGPNSRVARA
                     LGLGQNSRYLFGIEYEYASVDIDEPDKLHCFISRRLARGYIGWVAHGVGVVQVGLARR
                     LRGQAPPPREAMAAFLERIAPIFDFRAMRPSSVRAGKIPCGGLVRGVAAQRALLVGDA
                     AGMVSPVTAGGIHTALKHGLAAGHAIADYLAGKREDPSGWFVDSYPRFRAKRLLRFLF
                     DHFQSDTAFNLLLATSPMRRAAGLVYFHHKGVFDPAIVADSRSPADRSAIVRSG"
BASE COUNT          590 a         1128 c         1095 g          498 t
ORIGIN      
        1 gataacgcgg gtgcgcagga cgtcacgctg acgggccccg gagtcgtaca tgttggtttt
       61 ggtcggaacg ctggtcgtga ttgtctgcgt gatgggcggc tacgtcatgt cacacgggca
      121 cctgctcgcc ctttggcagc cctacgaatt gatgatcatc tttggcgggg cggcgggcgc
      181 cttcctcacc tccaaccccg gcaaggtggt gaaatccgcc ctgcgcgaag tggccggact
      241 cgccaagggg ccgacgtaca agaggcagga ctacgtcgac ctgctctcgc ttctgtatga
      301 catcttcgcg gtgatgcgca agcagggcgt gctcgcgttg gaaccgcaca tcgaggatcc
      361 ccagtcgagc tcggtattcg cggcctaccc gcggctgttg cgcgagcact acctcgtcga
      421 attcatcacc gactgcctgc ggctgatcgt cggcggcaac atggagccac acgagctgga
      481 ggcattgctc gacgccgaag tcgagaacca ccacgccgag gccgaggcgc cggcccaggc
      541 ggtggcgaag atggccgacg ccctgcccgg cttcggcatc gtggcggcgg tactgggcat
      601 tgtcaccacc atgtccaacc tcaccggcga cacctcccag atcggcgagc acatcgccgg
      661 cgcgctggtc ggaaccttcc tcggcatcct gctgtgctac ggcttcatcg gcccgctggc
      721 cgcggccatg gaagcccggg tgcgcgagga tggcaaggcc ttcgaatgtg tcaaggtcgc
      781 gctgctcgcc agcctgcgcg gctacaaccc gaaggttgcc gtggagttcg cgcgcaaggc
      841 gctctccgcc cacagccgcc cgaacttcca ggacctcgag gagcacctga agggtgcgcg
      901 cgccggggcg cccgcatgag cgaggacaag gccgccccga tcatcgtcaa gcgggcgcgg
      961 aagggtggtc acggccatca cggcggcgcc tggaaggtgg cctacgcgga tttcgtcacc
     1021 gcgatgatgg cgttcttcct ggtgatgtgg atcatcggcg tcggcacccg ggagcagaag
     1081 gccgcgatcg ccgaatattt ccgcaatccg agcgcgacct tcggtaccgc caccgtcgcg
     1141 ccacccggca aggacggccc cggcggcgcc agcgactcga tgatcaagct cggtggcgcc
     1201 atggacctgt cccacggtcc gggcaaggac cgccacaacg gtcccgtcgg cgccgcttcc
     1261 aaggaggaga tggacgagcg cgcgcgcgaa cgcgaccgcc agcggctgga ggaactgaag
     1321 gtccagctca acgccgcgat ccagaacagc caggcgctgg cgccgtacaa ggaccagttg
     1381 ctgctggaca tcacccccga gggcctgcgc atccagatcg tggacaagct caaccggccg
     1441 atgttcgacc tgggccgcga ccggctcaag gactacacca agacgatcct cgaagagcta
     1501 gccatcttca tcaacaaggt gccgaatcgc atcaccatca gcggccacac cgacgacgcg
     1561 ccgtacaaca acgaccacgg ctacagcaac tgggaattgt ccgcggaccg cgccaacgcc
     1621 gcgcggcgcg ccctgcttgc cggcggcctc gatccggcca agatcgcgca ggtggtgggc
     1681 ctggcggcgg cggttccgct cgacaaggcc gatccgcgcg atccgatcaa ccggcgcatc
     1741 agcatcatcg tgatgacgaa gcaggccgca gcggccgtgc tgtcgcagac cacctacgat
     1801 gcgccggcct cgggtcccct tccgcagtca ctgggaaccg cgctgcacgg ctgaacgcgc
     1861 gtttcgaggt cagccccccc agtgcctcgt cccacaaata acttcgtcac tccggacgag
     1921 cccacggagt gggtgagatc cggaatctgc ttcgaactgc tacaacggca gattccgggt
     1981 tcccgcgccg gcaagccggc gccggccccg gaatgacgat gtaaagattc tttcgggcga
     2041 cactagccgc tccggacaat ggccgatcga tcggccgggc tgcgcgaatc cgcaacgatc
     2101 gccgggtcga acacgccctt gtggtggaaa tacaccagcc cggcggcgcg gcgcatcggc
     2161 gatgtcgcca gcagcaggtt gaatgccgtg tcgctctgga agtggtcgaa gaggaagcgg
     2221 agcagccgct tggcgcgaaa gcgcgggtag gaatcgacga accagccgct ggggtcttcg
     2281 cgcttgcccg caaggtagtc ggcgatggcg tgtccggccg cgaggccgtg cttcagcgcc
     2341 gtatggatgc cgccggctgt caccggtgaa accatgcccg ccgcatcgcc caccagcaac
     2401 gcgcgctgtg ccgcaacgcc gcgaacgaga ccgccacagg ggatcttgcc ggcgcgcacc
     2461 gagcttggcc gcatcgcccg gaaatcgaag atcggcgcga tccgctcgag gaaggccgcc
     2521 atggcttccc ggggcggcgg tgcctggccg cgcaagcgcc gggcgaggcc gacctgcacg
     2581 acaccgacac cgtgggcgac ccagccgatg tagccacgcg ccaggcggcg gctgatgaag
     2641 caatggagct tgtcgggctc gtcgatatcg acgcttgcgt attcgtattc gatgccgaac
     2701 aggtagcggc tgttttgccc caggcccagc gcccgggcga cgcgggaatt cggcccgtcg
     2761 gcacccacga ggaaccgcgt ctgcccgagt tcgcccaggt cgaaaccgct gcgttggcga
     2821 tgcgcctcgc ggaacacctg gccacaatgg agcctggcgc ccgcctcctc cgcccgcccg
     2881 gcgagccagc gcatgacatt gggcgtgtcg gtggcgagga agtagtagcc gggcgcagcg
     2941 agatcgacgt gccgcaggtt cggggcatac agccgcacgc ccgagatgcg gcgcacgagc
     3001 tgcgcaggca ggtcatccag cagcgcgatc tggtcgatcg catccttgac gatgatcccg
     3061 gtggtatgca gtttctcgcc cggatcgcct ttctgctcgt acacggcgac ccgtaggccc
     3121 gccccggcca gggcgtgggc gcaggcaagt cccgcgaagc tcgcacccac gaccacgcaa
     3181 tcgtgctgga actgggtgga tcgggtcatc gggcgctccc cgcgaactcg atcgactata
     3241 gcagtcgcgg cacccaatga aagcggattg ccgcgaccct tcgcgggtgt cgccgatctt
     3301 tacaaaaccg g
//
LOCUS       DASSNH010000281         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_280398, whole genome shotgun sequence.
ACCESSION   DASSNH010000281 DASSNH010000000
VERSION     DASSNH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280398"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VFK00_10315"
     CDS             complement(<1..1107)
                     /locus_tag="VFK00_10315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7593383.1"
                     /translation="MSERETMEYDVVVVGAGPAGLAFAIRLKQLQSDARVCVIEKAST
                     IGAHILSGAVIEPAPLDDLLPGWRDNPPPICVPATHDEFLLLRDAKRAWKLPTPPQMN
                     NHGNFIVSLGALCAWLAPQAEALGVDVFPGFAAAEAVFDDAGAIKGVRIGDMGVARDG
                     SQKPGYTQGIDILAKLTVLAEGCRGSITKTLVAQYGLDRNSDPQTFGIGLKELWQVPE
                     GRVQPGHVTHTVGWPLDSRTYGGSFIYHLDKDRVAIGFVAGLDYPDPLFRPWEAFQQL
                     KQHARVRPLLEGGQIISAGARALVEGGLQSLPTTEMPGAILIGDAAGLMNVPKIKGTH
                     QAIRSGMLAAEHYAEKQSGEGFDARLRASSVAAEM"
     gene            1297..2724
                     /locus_tag="VFK00_10320"
     CDS             1297..2724
                     /locus_tag="VFK00_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7593384.1"
                     /translation="MGVAEEKFLDPAALRGGGVWQCLHWLASGRTTADALAEAYLGAI
                     ERLDPRLHAFVDVRPDIVRDQAASAQRRRREGPIGRLDGIPVAVKDNFDVAGYPTRAG
                     LPSRGSDIARHDAHAIARLRAAGAIILGKTNLDEGALGAATCNPHFGTTRNPCNAAYV
                     AGGSSGGSAAAVAAGLAPVAIGSDTLGSVRIPASYCGVFALKPTHGEISARGLVPAAR
                     RLDAVGILARSVGDLVVMLQVLAGYDADDPRSRRRRVALQPPDWNPTQLRCGVLPDLA
                     EAGVEPAVRDVFDAAVAKLEHQLGDRQSLDFSDWPYSKMRRAGLLLMEAEMRNTFAAD
                     LADSEHPASAEFRKMLDFAAGKSAADYVVADRMLDFAVLKARRVFSRVDVLVLPTTPQ
                     GAFPVDAPAPASQADLTSFASLSACPAVSIPMGTLPDGMPIGMQFVGRPGSDLRLLEL
                     AEVCASALDAAPAYPVEATTETAGN"
     gene            complement(2943..>3653)
                     /locus_tag="VFK00_10325"
     CDS             complement(2943..>3653)
                     /locus_tag="VFK00_10325"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7593385.1"
                     /translation="GAKPLAGFPGELVTEGLDGLRARLNEYVELGAQFAKWRAVITIG
                     DDMPSSTCIDANTHALARYAALCQEAGLVPMVEPEVLMDGDHDIETCYEVTEATLRSL
                     FAALYEHNVMLEGTILKASMVLSGKDCPEQADTEEVAESTILCLKSSVPAVLPGIVFL
                     SGGQSDADATAHLNEMNRLGPHPWPLSFSYGRAMQQAALHLWAQDLEGNFGAAQKIVV
                     ERARDNGLAALGQWKKAA"
BASE COUNT          532 a         1227 c         1279 g          615 t
ORIGIN      
        1 catttccgcg gcgacggacg atgcgcggag gcgcgcatcg aagccttcgc ccgattgctt
       61 ttcggcgtaa tgctcggctg ccagcatgcc cgagcggatc gcctggtggg tacccttgat
      121 cttcggcacg ttcatcagcc ctgccgcatc gccgatcaaa atcgcgccag gcatttcggt
      181 cgtcggcagc gattgcagtc cgccttccac cagcgcgcgc gcgcccgccg agatgatttg
      241 cccgccttcc agcagcggcc ggacacgcgc gtgctgcttc agttgctgga acgcttccca
      301 cggccggaac agcgggtcgg ggtagtcgag cccggccacg aagccgatcg cgacgcggtc
      361 cttgtcgagg tggtagatga aactgccgcc gtaggtgcgg ctgtccagcg gccacccgac
      421 ggtgtgagtg acgtggcccg gctgtacgcg cccctccggg acctgccaga gttccttgag
      481 cccgatgccg aaggtctgcg gatccgaatt ccggtcgagc ccgtattgcg cgaccagggt
      541 cttggtgatg ctgccgcggc aaccttcggc aagcaccgtg agctttgcga ggatgtcgat
      601 gccttgcgtg tagccgggct tctgcgaacc atcgcgggca acgcccatgt cgccgatccg
      661 cacgcccttg atcgcgcctg catcgtcgaa caccgcctcg gccgccgcga agcccgggaa
      721 cacgtccacg ccgagcgcct cggcctgcgg cgccagccac gcgcacagcg cgcccagcga
      781 aacgatgaag ttgccgtggt tgttcatctg cggcggagtg gggagtttcc acgcgcgttt
      841 agcgtcgcgc agcagcagga attcgtcgtg ggtagcgggc acgcagatcg gcggcgggtt
      901 gtcgcgccag cccggcagca ggtcgtcgag cggcgccggt tcgatcaccg ccccggaaag
      961 gatgtgcgcg ccgatggtcg aggccttctc gatcacgcag acccgggcgt ccgattgcaa
     1021 ctgtttcagg cgaatcgcga aggcgaggcc cgcgggtcct gcgccgacca cgaccacgtc
     1081 gtattccatc gtttcgcgct cgctcatcgt ccactccgtc gtttggtagg agcggccaat
     1141 ggccgcgacc gatattcagt tctgccgcaa ccccggttgc gcgcatggcg cgctactacg
     1201 gtgctcctcc gattgtcgag gtttgcggta cggtcggcaa accgggcgcc gctcccgcat
     1261 aatccggggt tcggaagcca tgatcaggga tagcggatgg gcgtagccga agagaagttc
     1321 ctggatcccg cggcgttgcg cggcggaggc gtctggcagt gcctgcactg gctggcctcg
     1381 ggccgcacca cggcggatgc cctggccgag gcgtacctcg gcgcgatcga gcgcctcgat
     1441 ccgcgactgc acgcgttcgt cgacgtgcgg ccggacatcg tgcgcgacca ggccgcgagc
     1501 gcccagcggc gccggcgcga ggggccgatc gggcgcctcg acggcatccc ggtcgcggtg
     1561 aaggacaatt tcgacgtcgc cggatacccc acgcgcgcgg ggctaccctc gcgcgggtcg
     1621 gacattgcgc gccatgacgc ccacgcgatc gcccgtctgc gcgcggcggg cgcgatcatc
     1681 ctgggcaaga ccaacctcga cgaaggcgcc ctcggcgccg ccacttgcaa tccgcatttc
     1741 ggcaccaccc gcaatccgtg caatgccgcg tacgtggcgg gcggttcctc gggcggatcg
     1801 gccgccgcgg tcgcggcggg cctggcgcca gtggcgatcg gttccgacac gctgggctcc
     1861 gtgcggattc cagcgagcta ctgcggggtg ttcgcgctga agcccactca cggcgagatt
     1921 tccgcgcgcg gtttggtgcc ggctgcgcgg cgtctggacg cggtcgggat cctcgcccgc
     1981 agcgtcggcg atctggtggt gatgttgcag gtgttggcgg gttacgacgc ggacgatccg
     2041 cgttcgcggc gtcggcgcgt cgcgctgcag cctccggact ggaatccgac gcagttgcgt
     2101 tgcggagtgc tgccggacct cgcggaggcc ggcgtcgagc ccgcggtgcg cgatgtattc
     2161 gacgcggcgg tggcgaagct ggaacaccag ctcggcgacc gccagtcgct cgatttctcg
     2221 gactggccgt attcgaagat gcgccgcgcc ggcctgctgc tcatggaagc cgagatgcgc
     2281 aatacctttg ctgcggatct ggcggatagc gagcatcccg cgtcggctga attccggaag
     2341 atgctcgatt tcgcggccgg aaaatccgcg gcggattacg tggtcgccga ccggatgctc
     2401 gacttcgcgg tgctgaaggc gcgccgggtg ttttcgcgcg tggacgtgct cgtgctgccc
     2461 accacgccgc aaggcgcatt ccccgtggat gcgccggcgc ccgcgtcgca ggccgacctc
     2521 acctcgttcg cgagcctgtc cgcgtgtccc gccgtcagca ttccgatggg caccctgccc
     2581 gatggaatgc cgatcgggat gcagttcgtg gggcgtccgg gatccgacct tcgcctgctg
     2641 gagctcgccg aagtctgcgc gtcggcgctg gatgccgcgc cggcatatcc ggtggaagcg
     2701 accacggaga ccgccggcaa ctgattgtcc gggcgtggag ccgggatgca gcgaagcgga
     2761 atccgggcta cgcgcggcgc cggctcgggg caggccggtt caaagagcca agcgatgact
     2821 gcgccgcagg agtggcgcga acggcaggcg ggcccgaagg gccggatgcc cggaatttac
     2881 cggcaagggt cagacaaaca taccggctcc gccctgcggg cttgagccgg cctacatcac
     2941 cgtcaggcgg ccttcttcca ctgccccagg gccgcgaggc cgttgtcgcg ggcgcgttcg
     3001 acgacgatct tctgggcggc gccgaagttg ccctccaggt cctgggccca aaggtgcagc
     3061 gcggcctgct gcatcgcgcg accataggag aacgacagcg gccacggatg cgggccgagg
     3121 cggttcatct cgttgaggtg cgcggtggcg tccgcgtcgc tctggccgcc cgagaggaac
     3181 acgatgccgg gcagcaccgc gggcaccgag gatttcaggc acaggatcgt tgactccgcc
     3241 acttcctcgg tgtcggcctg ctcggggcag tccttgccgg acagcaccat gctcgctttc
     3301 aggatcgtgc cttcaagcat cacgttgtgt tcgtacagcg ccgcgaacag cgagcgcagc
     3361 gtggcttcgg tcacctcgta gcaggtttcg atgtcgtggt cgccgtccat cagcacctcg
     3421 ggttcgacca tcggcacgag gcccgcttcc tggcacagcg ccgcgtagcg cgccagcgcg
     3481 tgggtgttgg cgtcgatgca ggtgctggaa ggcatgtcgt caccgatcgt gatcaccgcg
     3541 cgccacttgg cgaactgggc gcccagctcg acgtattcgt tgaggcgcgc gcgcaggccg
     3601 tccaggcctt cggtgacgag ctcgcccgga aaaccggcca gcggcttggc gcc
//
LOCUS       DASSNH010000282        21535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1524617, whole genome shotgun sequence.
ACCESSION   DASSNH010000282 DASSNH010000000
VERSION     DASSNH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 21535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21535
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524617"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1282)
                     /gene="gcvT"
                     /locus_tag="VFK00_10330"
     CDS             complement(134..1282)
                     /gene="gcvT"
                     /locus_tag="VFK00_10330"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7593386.1"
                     /translation="MTEPINNSAIRDDRINKAKHTVLNASHRAAGAKMVDFGGWDMPL
                     HYGSQLEEHHAVRRDAGMFDVSHMTVVDLSGARCREFLRHLLANDIGKLKKPGKAIYS
                     CMLNERGGVIDDLITYFLAEDFFRVVVNAATRGKDLAWIERQAAPFGVEVDERADLAM
                     IAVQGPNARAKVLALLDAGDRERVEKLGRFAAAEARGPDGMPLFVARTGYTGEDGFEI
                     IVPEQHAVELWEALAAAGVHPAGLGARDTLRLEAGMNLYGQDMDEDATPWEAGLAWTV
                     SLDEGRDFIGRAALERQQAAGVPRELVGVVLDEKGVLRHGQQVRAGAGEGAILSGIFS
                     PTLGKAIGFARVPAGARDGLRVDIRGRELPLRVVKYPFVRNGKPGEGV"
     gene            1435..2592
                     /locus_tag="VFK00_10335"
     CDS             1435..2592
                     /locus_tag="VFK00_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7593387.1"
                     /translation="MDHPTTPAGLVEWLPLPETGENVLRFTGDWTLAHFSRLEAQLDA
                     LKPELPEVPDVDFNDIGRLDTAGAGLIAVALGPKALHWLADHDKDVPRELRALLGTVS
                     DAVAELYAHRPPQPRNFGFIDMLADVGGSVVRIRKLAFKLLGFVGMTLETWARTLLRP
                     DRWRMTSLIAHMEQTGFHAVPIVALLSFLIGAVIAFLGATALQRYGASVFTVDLVSYA
                     FLRELAVLLTAIILAGRTASAFTAQIGSMKVGEEVDAMRTMGLDPIELLVLPRVIALM
                     IVTPMLTFLAMLAGMIGGLLVCWFALDITPAMFVSVFRADTPLYYLWLGLAKAPIFAF
                     LIAVIGCLEGFKVEGSAQSVGERTTSSVVQSIFLVIAVDAVAALFYMEMGW"
     gene            2589..3386
                     /locus_tag="VFK00_10340"
     CDS             2589..3386
                     /locus_tag="VFK00_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7593388.1"
                     /translation="MSALASEPARDTLVEVRGLRSQFGAHVVHEDLDLDVYRGEILGV
                     VGGSGSGKSVLLRTVLGLRRPDAGSVRLFGENLHALPPRRRIAIERRCGVLFQNGALF
                     SSLTVSENVRVPLMEHTSLSVHDARRVAALKIALADLAPEVRQRYPAELSGGMRKRAA
                     LARALALDPDIVFLDEPTSGLDPIAAASFDQLIRTLRDSLGLTVFMITHDLDSLHAIC
                     DRVAVLAHKRVIVADSLDKVERFDDPWIRDYFQGPRGRAAEAIHPLA"
     gene            3396..4322
                     /locus_tag="VFK00_10345"
     CDS             3396..4322
                     /locus_tag="VFK00_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7593389.1"
                     /translation="METRAHHVLIGTFTLVVVVVAVLFALWLGKASLSKQHHYYDIVF
                     TEAVTGLSTGSPVQYNGIQVGQVSQLKLDPKDPRKVLARIQVAADTPIKTDTRAKLGL
                     LGLTGVAFIQLSGGAPDSALLMPTPDNPVPQIKSENSALSQLLASGSDVVTNLNGILD
                     RLGQVVSQSNVDRITSTLKNIDQTTSTLAAERDDLRKLIHEASEASVKLNQTLAGANK
                     LVNGPALQTMERAQAAMTSLQQTTKTLNDLLVENKDSLQSGLRGVDQIGPTLRQLRAT
                     LRDIQQVTDQLQANPAAYLLGRDHPAEFTPKK"
     gene            4334..4993
                     /locus_tag="VFK00_10350"
     CDS             4334..4993
                     /locus_tag="VFK00_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HET7593390.1"
                     /translation="MHTSNGFRAPMRVLAAAFCAAALASACSILPKNQVVQVWQPPEN
                     ATVQPAKTPADFSLRVDTPNLAGTLDQQGIVVMPEPGQVSVYKGARWSESPALLVRGR
                     LVDAFMAARLRSVTTDDDRFASDYTLSGDLRAFQSEYRGGSPVVVVRYDAQLRRAGSR
                     SVLASRSFVLTEQPAGTQVPAVVAAFGKADDALAEQVVEWVLATANRNPQSPPGDAAS
                     R"
     gene            5088..5927
                     /gene="metF"
                     /locus_tag="VFK00_10355"
     CDS             5088..5927
                     /gene="metF"
                     /locus_tag="VFK00_10355"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET7593391.1"
                     /translation="MIPISLEFFPPKTEEQRAQMERALPKLKVLEPEFVSVTFGAGGS
                     TLSHTPRTVRHLREAHGLEVAPHVSCMGGSREEIRALLADYRELGCKRIIALRGDLPS
                     GMASPGDFRYASDLVAFIRREHDGWFHIEVACYPECHPQSDHAAADLRHFKAKVDAGA
                     DGAITQYFFNADGYFRFVEDARRAGVTVPIVPGVMPIANFTQLRRFSEMCGAEIPRWI
                     GKRMTAFGDDADAVREFGADVVAAMCRRLLEGGAPGLHIYTLNRARATRAVLERLDQP
                     LAA"
     gene            5957..6538
                     /locus_tag="VFK00_10360"
     CDS             5957..6538
                     /locus_tag="VFK00_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593392.1"
                     /translation="MSPWIVRLLCLAALGTWAPLAWSQAPAQTPIYRCVGAHGEPKFS
                     SQPCGTPVQLAGDGAGADAITGSYGFGDVCPASPEALRQAIAGAFESHDVNRLAGVIV
                     WRGIDQDSARTLLRTLADWLKQPLAGIAIAGSAEPPYAATGPAPAASVFHAGGNGSMM
                     APALAGFEISTAGGGTRAFGVTESDGCWWLTFD"
     gene            6686..7489
                     /locus_tag="VFK00_10365"
     CDS             6686..7489
                     /locus_tag="VFK00_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HET7593393.1"
                     /translation="MLKLFCALALSLVAMCACSSDRSAAVQEAHAAPAAPATTISPAV
                     RAGIAATVTEMAPGTKPQSIEPMPIAGLYQVVVQGQVLYMTGDGRYVIQGEAFDVASR
                     KSFNSQTLNRLRRDAIAGLSPDRMIRYAPANPKYTVTVFTDVDCPYCRAFHANIAEIN
                     KLGIAVNYLFWPRTGLGTPSARKAVDVWCAGDRRDALTRAFEGKAPPRANCESPVEHD
                     FDLGVDLGVDGTPTIIADNGVVLGGYVDPKELLRRLQSVRATPADPRSR"
     gene            7647..11579
                     /gene="purL"
                     /locus_tag="VFK00_10370"
                     /gene_synonym="purI"
     CDS             7647..11579
                     /gene="purL"
                     /locus_tag="VFK00_10370"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET7593394.1"
                     /translation="MIVLDGRSALSDFRLDRLNRRLDALSKGTRVRGARHVYFVQSDS
                     SLTPALHRRLAEVLDATEAPPADASLWVVPRFGTRSPWSTKATDIVHHVGIHVSRVER
                     GTAFLIDRLPEAGDPAFAAVRAVLHDPMTQSVMPRLGDAVRVFASGEAGPHQTLALGN
                     DAKAALREANAELGLALSDDEIEYLATHYAKLGRDPTDAELVMFAQANSEHCRHKVFN
                     AEFTVDGEKQDATLFGLIRHTHRASPAYTLSAYSDNAAVIEGNEGARFFADADGVWRA
                     HHEAIPYAIKVETHNHPTAISPWPGAATGSGGEIRDEGAVGRGGKPKAGLTGFSVSHL
                     RIPELPRPWEEARPLPPRVASAFEIMRDGPLGAAGFNNEFGRPCLTGYFRTFELETGK
                     PGMRHGYDKPIMLAGGVANVRPMLVKKNKLDPGDAVIVLGGPAMLIGLGGGAASSMAA
                     GTSSEALDFASVQRDNPEMQRRCQEVIDACCAQGEASPIVAIHDVGAGGLSNAIPELL
                     HDAGVGGEIDLDAVPSDDPQLSPMQIWCNESQERYVLGVKPDDLAGFEAICKRERCPF
                     AVVGHATEEERLLVTSQKREQGTGNREAATTLHAPSPFPVPGSRFTVIDLELAMLLGK
                     PPRMQRDATRIKPHLEIIPDLSNIGLDDAIERVLRMPAVASKAFLIHICDRSVGGLCS
                     RDQLVGPWQVPVADCAVTLADFEGHAGEAMAIGERSPVAVLDGAASARMALGEAITNL
                     LAAPVDKLEEVRLSANWMAAIGHAGEDAALYDAVRAVSTQLCPALGISIPVGKDSLSM
                     RTLWREDGREQRTIAPVSLIVSAFVRIADTRRTLTPQLRMDAGETELWLVDLGAGRNR
                     LGGSALTQAYDRRGGVPPDFEDPERLRALFQLVRTARKQDLLLAYHDRSDGGAIVTLL
                     EMAFAGHCGLEIFLDDWADNALLALFNEELGAVVQVRKDRHDAFAALVEANGLESMTR
                     VIARPKAKLGIKLHSGDETVAKWNWQKLIGAWHETSHAMQRLRDNPASADAERAWRCD
                     DNDPGLNARLTFDPAADVAAPFIAKGARPRIAILREQGVNGQVEMAAAFTRAGFDAVD
                     VHMSDLVAGRHHLADFTGFAACGGFSYGDVLGAGRGWAASILYHGDLRDQFAAFFADE
                     SKFAVGMCNGCQMLSHLKEIIPGAEHWPTFARNASEQYEARFVTLEVLDTESVVLRGM
                     AGSRIPVVTAHGEGRAVFADDHKAGKADACVRFIDNRGKPTEAFPCNSNGSPEGLAGF
                     TAARGRVTILMPHPERVFRTAQMSWHPREWGEDSPWMRLFRNARVFVG"
     gene            complement(11606..12898)
                     /locus_tag="VFK00_10375"
     CDS             complement(11606..12898)
                     /locus_tag="VFK00_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7593395.1"
                     /translation="MQTFESFLDQLWVRTALDCVLLLLVAWLAGRLVRYAMVHVMRLA
                     SRHTTWRWDDAMVKRRVFLRLSQVVPMLIVNAGLHAVPGVPEAAEKVVAAITTALIIV
                     FVVLAVDAALSAMEDLYNQTPRGRERPIKGYVQLLKIVLFVVGLILIIAALFQRDPLT
                     LLAGLGAVSAVLLLIFKDTIMSVVASVQIGSYDMLRVGDWISMPEDNVDGDVVEISLN
                     TVKVVNWDKTLSTIPTWHLISKSYRNYRNMYETGRRIRRALNIDITSVRYHTPGEIEH
                     LQRFRLLDDYFQQKSSELAAWNADLGREGADAINQRRLTNLGSFRAYMQAYIGSHPGI
                     NQDLFWAVRQLDPAPTGIPLQVYAYTRNTSFVPHENVQGDIFDHLITILPEFGLMLFQ
                     QPSGCDMRAGFARHDRPRLDEDGARAEDHEPLEQLAPP"
     gene            complement(13216..13608)
                     /locus_tag="VFK00_10380"
     CDS             complement(13216..13608)
                     /locus_tag="VFK00_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7593396.1"
                     /translation="MHRSRLCSILIDCRTDDLESAARFWSEALGRPLREGTNPGGSNY
                     IGMVTHDDEPVIEVQKVDHDSRVHLDIESNDIEAEVRRLEALGAKRVGSIKTWVVMEA
                     PTGQRFCVVRPQRGQIGPHANEWPDPLD"
     gene            complement(13644..14060)
                     /locus_tag="VFK00_10385"
     CDS             complement(13644..14060)
                     /locus_tag="VFK00_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7593397.1"
                     /translation="MAGNNGSWARTAPYLLSILRIIAAFLLMQHGAEKLFGYPGGSFE
                     LISSAKLFTIVPGLAGILEFGGGFLLLIGLFSRPVAFILSGEMAFAYFMAHFPHNHVV
                     PILNGGGLAVLFCFTFLYLSAAGPGPWSLDRALRRA"
     gene            complement(14146..15447)
                     /locus_tag="VFK00_10390"
     CDS             complement(14146..15447)
                     /locus_tag="VFK00_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET7593398.1"
                     /translation="MIQPAEFARRRRELMRMAGEDAALVVAAAPRRIRNADAEWPYRQ
                     DSDFHYLTGFPEPDAVLALLPGRANGEVVLFCRERDPERERIDGARIGPDAAAARFGM
                     DDAFPVEDIDDILPGMLEGRTRLYCHFGRENEFDAHLLRWLRRLRASRGGGVVPREFV
                     ALGHLLHELRLYKSTAELRLMKRAANIAAEAQRAAVAAARPGAAEYEVEAALLHVVRA
                     HGAVPSYAPIVAGGANACVLHYTANRDRLERGDLLLVDAGAEVECYASDITRTWPVGG
                     RFSREQRALYEVVLAAQQAAIEQVREGAAFTAAHDAARKVVVEGLCQLKLLSGSPRSA
                     LKSGSYERFFPHKTGHWIGLDVHDVGDYRVDEQPRLLEAGMVVTVEPGIYIPPDDTTV
                     PAKWRGIGIRIEDDVAIGAHGSEVLTAALPKAIDEVQALAY"
     gene            complement(15444..15974)
                     /locus_tag="VFK00_10395"
     CDS             complement(15444..15974)
                     /locus_tag="VFK00_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HET7593399.1"
                     /translation="MTRMREVSYTEFAKAVARARLGIDAAELHGSAAGYLCAGGGGDG
                     HVLLSALQLESDDAGALDPLHALLDELAAGMSRDLGAGEAVAPLLPEAPLAARADGMV
                     DWCRGFLGGLGLTGALEGSALDAGTRELLDAFGQIASMRIECDEGDEGALDDVLEFIR
                     GGTVQLHARVARAVRR"
     gene            16202..20800
                     /locus_tag="VFK00_10400"
     CDS             16202..20800
                     /locus_tag="VFK00_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7593400.1"
                     /translation="MSRVPTGPSGPTPPAARSHILPALACVLLWFAGVATAAQAPPAT
                     WQAYQRTFYFQQLDSADGLAQNSASVVFQDHRGFMWFATQGGLFRYDGYKLTRYTHDP
                     DQDDGLPGGFPVSSLADGPDGKLWVGSSSGGLVLLDPDGGTVVPLPKGVERSDLPVTS
                     LLGLADGDVLIGSRLGVDRLVAAPRPHLQRAWAESQGMAEPVHALTSCPDGAVFGVAG
                     DSIIALDAKAGHGRILATTQQPVDALHCDAASHRLLLGNRDGLASVDRKSGALVWLWR
                     SDGDSAVGVRDIAADQAGRVWLALSDRSLLRLGPDGAEQEVVPAPVGITGGLPDSRID
                     TLFVDRTGLLWVGTLSAGVVWTHPDGNPIQAVLNLSGDPRAHHYIRGFHADGDGRYWV
                     ALSGPGLVRYDRATHTITGFGSALAEAVASVPHAPVAASTSAAPATAIRSFGVIPDSD
                     GTLLVPSSAGILRFDPATGKASYADLDGKPATTAVRALYRAHNGDLWVAYANQGLVHY
                     HEGQALQRFDADHGLGSNQVLVLDEDSHGNIWAGTTRGLSMIEPASGKLRTFREIPGR
                     DDSLAGSTISSLLVAADGQLWVGSLSGISRLQGIDAAGAHFQRYGLDEGLPDNTIDCM
                     LEDASGDIWFSTNLGIGRLDPKTGAIRNFGASDGLQGSEYNSGACLRTREGRLLFGGH
                     GFDIIDPAKVRPSTTQAPVVITDLYDAGHRMAVPASSSARIDIGPKDRSLYISFAALD
                     YGSPGHSRYRFRLLGRDARWSEPTQDHSVAYSSLAPGDYRFEVQSVLRDGRAGSTAAV
                     AVHVAATWWWSPVMQVVYAAAIVLLVLIAIFGWRSHHHEQQRHRRQLRLRESRLRQAL
                     WGSGDEFWDWNLESGTILRLGPEGAPGGHHEQAVPADEWRRTMVHPDDLASLDHALAE
                     HLAGNTEHFEVEHRVRGRDDEWTWVLSRGKVVERDAGGKPLRVCGTERDISAIRAADR
                     DRRIASEVIRNMSEAVTVTDLDFRFVSVNTAFTRMTGYAEHEVLGRDASVLNGSRHAP
                     ESYTHLRQMLAETGHWRGELWQRRKDGEEFLCWVELTEVCDASGQRTHMIGVLTDITD
                     RKRAEQELRYLANYDTLTGLPNRALLGERLGAALISARRSGHKVALLFLDLDRFKHVN
                     DSLGHTAGDRTLKAAGARLRQCVRENDVVARLGGDEFTVVLEDLADVGGAEEMAQRLI
                     HAFAKPLQVSGTQETVISPSIGIAMYPDHGQTPADLLKCADTAMYQAKSSGRNTWAVY
                     HAGMDARARDHATLVNALRRALDRGELRLVYQPKMSLHGENITGMEALLRWHNPDLGE
                     IPPSVFIPLAEETGQIHEISAYVFGTACADLRRFRENGHRNLTMAINLSAAQLNRPDL
                     TSHICDLLAEHDVAPNQVELELTESMVMANAEQSVRLLGELKSIGTRLAIDDFGTGYS
                     SLAYLKRLPIDTLKIDQEFVGDITTDPDDAAITSTIITMAHSLQLKVVAEGVETLEQL
                     AFLRAQHCDEIQGYWFSRPLSAEACLDFLNRHAVANVV"
     gene            20846..21073
                     /locus_tag="VFK00_10405"
     CDS             20846..21073
                     /locus_tag="VFK00_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HET7593401.1"
                     /translation="MDNPAPTVEDELAALTRTLDRALERLRRLAEENASLRRGRDQLA
                     GERAALMTRNEQARARVEAMIERLKALESAG"
     gene            21159..21464
                     /locus_tag="VFK00_10410"
     CDS             21159..21464
                     /locus_tag="VFK00_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7593402.1"
                     /translation="MAEESVTVNVKLLDREFLVGCKPDEREGLLAAVEHLNRKMREIR
                     HSARSPGYDRIAVLAALDITHDLLMLQRSQNRLSSTAGEQIAMLRRKLEDALEAPIE"
BASE COUNT         3509 a         7582 c         7131 g         3313 t
ORIGIN      
        1 gaaaatgcaa gccaacgtcg ccatcatgcg caagacgctc accgccgcct gggccatcat
       61 gaaagacccg aagccttacg acacgacgct cctataccca gacctcgaaa ccacttgacg
      121 cggaacagag tgtctacacg ccttcgccgg gtttgccgtt ccgcacgaac gggtacttca
      181 ccacgcgcaa tggcaattcg cggccgcgga tgtcgacgcg caatccgtcc cgcgccccgg
      241 cggggacccg cgcgaacccg atcgccttgc ccagcgtggg cgaaaagatg ccggagagga
      301 tcgcgccctc gccggctccc gcacgcacct gctgtccgtg gcgcagcacg cctttctcgt
      361 ccagcaccac gccgaccagt tcgcgcggca cgccggcggc ttgttgcctt tcgagcgccg
      421 cgcggccgat gaaatcgcgg ccctcgtcca gcgacaccgt ccacgccagg cccgcttccc
      481 acggcgtggc gtcctcgtcc atgtcctgcc cgtacaggtt catgccggcc tcgagccgaa
      541 gcgtgtcgcg cgcaccgagg cccgccgggt gcacgcccgc cgcggccagc gcctcccaca
      601 gttcaactgc atgctgttcg ggcacgatga tctcgaatcc gtcctcgccc gtgtagccgg
      661 tgcgcgccac gaacagcggc atgccgtccg ggccgcgcgc ttcggcggcg gcgaaacggc
      721 cgagcttttc cacgcgctcc cggtcgcccg cgtcgagcag cgccagcacc ttcgcgcgcg
      781 cgttcggtcc ctgcaccgcg atcatcgcga ggtcggcgcg ctcgtccacc tcgacaccga
      841 acggcgcggc ctgccgctcg atccacgcga ggtccttgcc gcgggtcgcg gcattcacca
      901 ccacccggaa gaagtcttcg gccaggaaat aggtgatgag gtcgtcgatc acgccgccgc
      961 gctcgttgag catgcacgag tagatcgcct tgcccggctt cttcagcttg ccgatgtcgt
     1021 tggcgaggag atgccgcagg aattcgcggc agcgcgcccc ggagagatcg accacggtca
     1081 tgtgcgacac gtcgaacatg ccggcgtcgc ggcggacggc gtggtgctcc tcgagttgcg
     1141 agccgtaatg cagcggcatg tcccagccgc cgaagtcgac cattttcgcg cccgcggcgc
     1201 ggtggctggc gttgaggacg gtgtgcttgg ctttatttat gcgatcgtcc ctgatcgctg
     1261 agttattgat tggctcggtc atctgattca cctctggtca tggggcatca cctcggccat
     1321 ccctggcctg atttttcaca acagccgctc cggctgatga tccattatcg tgcgcaaagc
     1381 cacagatcgc gcgcgcgcaa tactttggcg gcccgacgct gctagattgc gcgaatggat
     1441 catccgacga ccccggccgg cttggtggag tggctgccgt tgccggaaac cggcgaaaac
     1501 gtgctgcgat tcaccgggga ctggacactg gcgcacttct cgcggctcga agcgcagctc
     1561 gatgcgctga agccggagtt gccggaagtc ccggacgtcg acttcaacga catcggccgg
     1621 ctcgacaccg cgggtgccgg gctgatcgca gtcgcgctcg gcccgaaggc gctgcactgg
     1681 cttgccgacc acgacaagga cgttccgcgc gagctccgcg cattgctcgg aaccgtcagc
     1741 gatgcggttg ccgagctcta cgcgcaccgg ccgccgcaac cacgcaactt cggattcatc
     1801 gacatgctcg ccgacgtcgg cggcagcgtc gtgcggatcc gcaagctcgc gttcaagctg
     1861 ctcggcttcg tcggcatgac cctggagacc tgggcccgga cgctcttgcg gccggaccgc
     1921 tggcgcatga cctcgttgat cgcgcacatg gagcagaccg gcttccacgc ggtgccgatc
     1981 gtggcgctgc tgtcgttcct gatcggcgcg gtgatcgcct tcctcggcgc caccgcgctg
     2041 cagcgctatg gcgccagcgt gttcacggtg gacctcgtgt cgtacgcgtt cctccgcgag
     2101 ctggcggtgc tgttgacggc aatcatcctc gccggccgca ccgccagcgc gttcaccgcg
     2161 cagatcggtt cgatgaaggt cggcgaggaa gtcgacgcca tgcgcaccat gggcctggat
     2221 cccatcgagc tgctggtgct gccgcgggtg atcgcgctga tgatcgtcac tccgatgctg
     2281 accttcctcg cgatgctggc cggcatgatc gggggcctgc tggtgtgctg gttcgcgctc
     2341 gacatcacgc ccgcgatgtt cgtgtcggtg ttccgtgcgg atacgccact ctattacttg
     2401 tggctggggc tggccaaggc tccgattttc gcgttcctga tcgcggtgat cggctgcctc
     2461 gagggcttca aggtggaagg cagcgcgcag tccgtgggcg aacgcaccac ctccagcgtg
     2521 gtccagtcga tcttcctggt gatcgcggtg gacgcggtcg cggcactgtt ctacatggaa
     2581 atgggctggt gagcgcgctc gcttccgagc cggcgcggga tacgctggtc gaagtccgcg
     2641 gccttcgcag ccagtttggt gcgcacgtgg tccacgagga cctggacctc gacgtgtacc
     2701 gcggcgagat cctgggcgtg gtcggcggct ccggctcggg caagtccgtg ctgctgcgca
     2761 cggtcctcgg cctgcgccgg cccgacgccg gcagcgtcag gctgttcggc gagaacctgc
     2821 acgccctgcc gccccggcgc cggatcgcga tcgagcgccg ctgcggcgtg ctgttccaga
     2881 acggggcgct gttctcctcg ctcaccgtca gcgaaaacgt ccgggtgccg ctgatggaac
     2941 atacctcgct cagcgtgcac gatgcgcggc gggtggcggc gctcaagatc gcgttggccg
     3001 atctcgcgcc cgaagtgcgg cagcgctacc cggcggaact ttccggcggc atgcgcaagc
     3061 gggcggcgct ggcgcgtgcg ctggcgctcg atccggacat cgtgttcctg gacgaaccga
     3121 cctcaggcct cgacccgatc gccgcggcct cgttcgacca gttgatccgc accttgcgcg
     3181 actcgctggg cctgaccgtg ttcatgatca cccatgacct cgactcgctg cacgcaatct
     3241 gcgaccgcgt ggccgtgctc gcgcacaagc gggtcatcgt ggccgactcg ctggacaagg
     3301 tggaacgctt cgacgaccct tggatccggg attatttcca ggggccgcgc ggccgcgccg
     3361 cggaagccat ccaccccttg gcatgaggta gcaagatgga aaccagggcc caccatgtgc
     3421 tgatcggcac cttcaccctc gtggtggtgg tagtggcagt gctgttcgcg ctgtggctgg
     3481 gcaaagccag cctcagcaaa cagcaccact attacgacat cgtgttcacc gaagccgtga
     3541 ccggactgtc cacgggcagt ccggtgcagt acaacggcat ccaggtcggc caggtcagcc
     3601 agttgaagct cgacccgaag gatccgcgca aggtgctggc gcggatccag gtcgccgccg
     3661 acacgccgat caagaccgac acccgcgcca agctgggcct gctcgggctg accggcgtcg
     3721 cgttcataca attgagcggg ggcgccccgg acagcgcatt gctgatgccc acccccgaca
     3781 acccggtacc gcagatcaaa tcggaaaatt ccgcactctc ccagttgctg gcctccggca
     3841 gcgacgtggt gaccaacctc aatggcatcc tggatcgcct cggccaggtg gtctcgcaga
     3901 gcaacgtcga ccggatcacc agcacgctca agaacatcga ccagaccacc agcaccctgg
     3961 cggccgaacg cgacgacctg cgcaagttga tccacgaggc cagcgaggcc tcggtcaaac
     4021 tcaaccagac cctcgccggc gcgaacaagc tggtcaacgg cccggcactc cagacgatgg
     4081 aacgcgcgca agcggcgatg acatcgctgc agcagaccac caagaccctc aacgacctgc
     4141 tcgtcgagaa caaggactcg ctgcagtcgg ggctacgcgg ggtcgaccag atcggtccga
     4201 ccctgcggca actgcgcgcc acgctgcgcg acatccaaca ggtcaccgac cagctgcagg
     4261 cgaatccggc ggcctacctg ctcgggcgcg accaccccgc cgaattcacg ccgaagaaat
     4321 gaggacgccg tccatgcata cctcgaacgg attccgcgcc ccgatgcgcg tcctggccgc
     4381 ggcgttttgc gcggccgccc tcgcgagcgc ctgctcgatc ctccccaaga accaggtggt
     4441 gcaggtctgg cagccgccgg aaaacgccac cgtccagccg gctaagacgc ccgcggattt
     4501 cagcctgcgg gtggacacgc ccaacctcgc cggcacgctc gaccagcaag gtatcgtggt
     4561 gatgcccgaa cccggtcagg tcagcgtcta caagggcgct cgctggagcg aatcgccggc
     4621 gttgctggtc cgcggccggc tggtcgacgc gttcatggcg gccaggctgc gctcggtcac
     4681 caccgacgac gaccggttcg cctccgacta caccctgagc ggcgacctgc gcgcgttcca
     4741 gtcggagtac cgcggcggct cgccggtggt ggtggtccgc tacgacgcgc aactgcgccg
     4801 cgccggttcg cgcagcgtgc tcgccagccg cagcttcgtg cttaccgagc agcccgcggg
     4861 cacccaggtg ccggccgtcg tcgccgcgtt cggcaaggcc gacgatgcgc tcgccgagca
     4921 ggtggtcgaa tgggtgctgg cgaccgccaa tcgtaatccg caatcgccgc ccggcgacgc
     4981 cgcttcgcgc tgaccgcctc ccgctgtagc cgccggcccc ggcgcggttg catccggcag
     5041 tccttctctc tatcttgatg gagagatata tggacgccgc cttgccgatg atccccattt
     5101 cgctggaatt cttcccgccg aagaccgagg agcaacgggc gcagatggag cgcgcgctgc
     5161 cgaagctgaa agtcctggaa ccggaattcg tcagcgtgac cttcggcgcc ggcgggtcga
     5221 cgctcagcca cacgccgcgc acggtgcggc acctgcgcga agcgcatggg ctggaagtcg
     5281 cgccgcacgt gtcgtgcatg ggcggttcgc gcgaggaaat ccgcgcgctg ctcgccgact
     5341 accgcgagct tgggtgcaag cgcatcatcg cgctgcgcgg cgacctccca tcagggatgg
     5401 cctcgccggg cgacttccgc tatgcctcgg acctggtcgc gttcatccgc cgcgaacacg
     5461 atggctggtt ccacatcgag gtggcctgct atcccgagtg ccacccgcag tccgaccacg
     5521 ccgcggccga cctccggcac ttcaaggcca aggtcgatgc cggcgccgac ggcgcaatca
     5581 cccagtactt cttcaacgcc gatggttact tccggttcgt cgaggacgca cgccgcgcag
     5641 gcgtgacggt tccgatcgtg cccggggtca tgcctatcgc caacttcacc caactgcgcc
     5701 ggttttccga aatgtgcggg gcggaaatcc cgcgctggat cggcaagcgg atgacagcgt
     5761 tcggcgacga cgcggacgcg gtgcgggaat tcggcgcgga cgtggtggcg gcgatgtgcc
     5821 ggcggctgct ggaaggtggc gcgcccggcc tgcacatcta cacgctgaat cgcgcgcgtg
     5881 cgacgcgcgc ggtgctggag cgcctcgacc agccactcgc ggcctgagcc tgcgcgccgg
     5941 gggctagact ggcgtgatga gcccgtggat cgtccgcctg ctgtgcctcg ccgctctcgg
     6001 cacctgggca ccgctcgcgt ggtcgcaggc accggcgcaa acgccgatct accgctgcgt
     6061 cggcgcccac ggtgaaccca agttctccag ccagccatgc gggacgccgg tgcaactcgc
     6121 cggggatggt gcgggcgcgg acgcgatcac cggcagctac ggcttcggcg acgtctgccc
     6181 cgcatcaccg gaggcgcttc gccaagccat tgcgggcgcc ttcgaaagcc acgacgtgaa
     6241 ccgtctggcc ggcgtcatcg tctggcgcgg catcgaccag gactccgcgc gcacgctgct
     6301 gcggacgttg gccgactggt tgaagcaacc gctggcgggc atcgcgatcg ccggttccgc
     6361 ggaaccgcca tacgccgcga cgggccccgc gcccgcggca tccgtcttcc acgccggggg
     6421 aaacggctcg atgatggctc ccgccctggc ggggttcgag atctcgaccg ccggcggtgg
     6481 cacccgcgcc ttcggcgtca ccgaatccga cggctgctgg tggctgacct tcgactgagc
     6541 cgatgccgtg aaggctgcgc gcgcgagctt cggcgggagc ttcggagggc gccgcgattg
     6601 cgccgcggat cacgacccgg tgcggacgcc gcgtgctagc atccacccgt cgcgcccgct
     6661 gcgatgcagc acctcgaaac acgccatgct gaaactcttc tgcgcgctcg cactgtccct
     6721 ggtcgcgatg tgcgcctgct cctccgatcg cagcgccgcc gtgcaggagg cccatgcggc
     6781 accggcggcc ccggccacga cgatcagccc ggcggtacgc gcgggcatcg ccgccacggt
     6841 caccgagatg gcgcccggca cgaagccgca gagcatcgag ccgatgccga tcgcgggctt
     6901 gtaccaggtc gtcgtgcagg gccaggtcct gtacatgacg ggcgatggcc gctacgtgat
     6961 ccagggcgag gccttcgatg tcgccagccg caagtcgttc aacagccaga cgctgaaccg
     7021 gctgcggcgc gatgcgattg ccgggctgtc cccggaccgg atgatccgct acgcgccggc
     7081 gaacccgaaa tacacggtga cggtgttcac cgacgtcgac tgcccctatt gccgcgcgtt
     7141 ccacgccaac atcgccgaaa tcaacaagct cggcatcgcg gtcaattacc tgttctggcc
     7201 gcgcaccggg ctcggcacgc cgtcggcgcg caaggccgtg gacgtgtggt gcgcgggcga
     7261 ccgccgcgat gcgctcaccc gcgcgttcga aggcaaggcg ccgccccgcg cgaactgcga
     7321 gtcgccggtc gaacatgact tcgacctggg ggtcgacctc ggcgtcgacg gcacccccac
     7381 gatcatcgcc gacaacggcg tggtgctcgg cggctacgtc gacccgaagg aactgctgcg
     7441 gcgcctgcag agcgtgcgcg cgacgcctgc cgatccgcgc tcgcgctgaa gcactcgctt
     7501 acctgaaaag tcaggccagg aagggccgtt ccggcacgag gccgcgcgct taaagcaccc
     7561 ggctcggata gctctgacgt ggagcagcga tcgtggacga tcgcacttat aattaccggc
     7621 tttccccggc ccgcgacccc atcgtcatga tcgtcctcga cgggcgcagc gccctgtcgg
     7681 atttccggct cgaccgtctc aaccgccgcc tcgacgcgct gtcgaaggga acgcgcgtgc
     7741 gcggcgcccg ccacgtctat ttcgtccagt cggattcgtc gctgacgccg gcgctgcaca
     7801 ggcgcctggc cgaggtcctc gatgcaaccg aggcgccgcc ggccgacgcg tcgctatggg
     7861 tggttccacg cttcgggaca cgctcgccgt ggtcgaccaa ggctaccgac atcgtgcacc
     7921 acgtcggcat ccacgtttcg cgcgtggagc gcggcaccgc gttcctgatc gaccggttgc
     7981 ccgaagcggg tgatcccgcg ttcgcggcgg tgcgagccgt gctgcacgat ccgatgacgc
     8041 agtcggtgat gccgcgactc ggcgacgcgg tgcgcgtgtt cgcgtcgggc gaagccggtc
     8101 cgcaccagac gctcgcgctc ggcaacgatg cgaaggccgc gctgcgggag gcgaacgccg
     8161 aactcggcct cgccctcagc gacgacgaaa tcgaatacct cgcaacccat tacgcgaagc
     8221 tgggacgtga ccccaccgac gcggaactgg tgatgttcgc gcaggccaac tccgagcact
     8281 gccggcacaa ggtattcaac gccgagttca ccgtcgatgg cgagaagcag gatgcgaccc
     8341 tgttcggcct gattcgccac acccaccggg cgtcacccgc gtatacgctg tcggcgtaca
     8401 gcgacaacgc cgcggtgatc gagggcaacg aaggcgcgcg tttcttcgcc gacgccgacg
     8461 gcgtatggcg tgcgcaccac gaagcgattc cctatgcgat caaggtcgaa acgcacaacc
     8521 atccgaccgc gatttccccg tggccgggcg cggccacggg ctcaggcggc gagatccgcg
     8581 acgagggcgc ggtcgggcgc ggcggcaagc ccaaggcggg actgaccggc ttttccgtgt
     8641 cgcacctgcg catcccggag ttgccgcggc cgtgggaaga ggcgcgtccc ctgccacccc
     8701 gggtcgccag cgccttcgag atcatgcgcg acggtccgct cggcgccgcc ggcttcaaca
     8761 acgaattcgg ccggccctgc ctcaccgggt acttccgcac cttcgaactc gaaaccggca
     8821 agccgggcat gcgccacggc tacgacaagc cgatcatgct ggccggcggc gtcgccaatg
     8881 ttcgcccgat gctggtgaag aagaacaagc tcgatcccgg cgacgcggtg atcgtgctcg
     8941 gtggtccggc gatgctgatc ggactgggcg gcggcgcggc ctcgtcgatg gcggccggca
     9001 cgtcgagcga ggcgctggac ttcgcctcgg tgcaacgcga caaccccgag atgcagcgcc
     9061 gctgccagga agtgatcgac gcctgctgcg cgcaaggcga ggcgagcccg atcgtcgcga
     9121 tccacgacgt tggtgcgggc ggcctgtcca acgcgatccc cgagttgctc cacgacgccg
     9181 gcgtcggcgg cgaaatcgac ctcgacgcgg tgccttccga cgacccgcaa ctctcgccca
     9241 tgcagatctg gtgcaacgag tcgcaggagc ggtacgtgct cggggtcaag ccggacgacc
     9301 ttgccggctt cgaagcgatc tgcaaacgcg agcgttgtcc gttcgcggtg gtggggcatg
     9361 cgacggagga agaacggctg ctggtgacga gccagaagcg ggaacaggga acagggaaca
     9421 gggaagcagc cacaacgctc cacgctcctt ccccgttccc cgttcccggt tcccgcttca
     9481 ccgtgatcga cctcgaactc gcgatgctcc tcggcaaacc gccgcggatg cagcgcgatg
     9541 cgacccgcat caagccgcac ctcgagatca ttcccgacct aagcaacatc gggctcgacg
     9601 atgcaataga gcgcgtgctc cggatgccgg cggtggccag caaggcgttc ctgatccaca
     9661 tctgcgaccg cagcgtcggc ggcttgtgtt cccgcgacca gctcgtgggc ccgtggcaag
     9721 tgccggtcgc cgactgcgcg gtgacgctgg ccgacttcga aggtcatgcg ggcgaggcga
     9781 tggcgatcgg cgagcgctcg ccggtcgcgg tgctggacgg cgcggcatcc gcgaggatgg
     9841 cgctgggcga ggcgatcacc aacctgctcg ccgcccccgt cgacaagctg gaagaagtac
     9901 ggctgtccgc caactggatg gcggcgatcg gccatgccgg cgaagacgcc gcgctgtatg
     9961 acgcggtgcg ggcggtctcg acgcaactgt gtcccgcgct cggcatctcg attccggtcg
    10021 gcaaggattc gttgtcgatg cgcacgctgt ggcgcgagga cggacgcgag cagcgcacga
    10081 tcgcaccggt atcgctgatc gtcagcgcgt tcgtgcggat cgccgacacc cgccgcacgc
    10141 tgacgccgca actgcggatg gatgcgggcg aaaccgaatt gtggctggtc gacctcggcg
    10201 ccggccgcaa ccggctggga ggttccgcct tgacccaggc gtatgaccgc cgcggcgggg
    10261 tgccgcccga cttcgaggat cccgaacgac tgcgcgcgct gttccagctt gtccgcaccg
    10321 cgcgcaagca ggacctgctg ctcgcctacc acgaccgttc cgacggcggc gcaatcgtga
    10381 ccctgctgga gatggccttc gccggccact gcggcctgga aatcttcctc gacgactggg
    10441 ccgacaatgc gctgctcgcg ctgttcaacg aagagttggg cgcggtggtg caggtgcgca
    10501 aggaccggca cgatgccttc gcggcgctgg tcgaggcgaa cggcctcgag tcgatgacga
    10561 gggtgatcgc gcgcccgaaa gccaagctcg gcatcaagct ccacagcggc gacgagaccg
    10621 tcgccaagtg gaactggcag aagctgatcg gcgcctggca cgaaaccagc catgccatgc
    10681 agcgcctgcg cgacaatccc gcgagcgccg acgccgaacg cgcctggcgc tgcgacgaca
    10741 acgatcccgg cctgaacgcg cggctcacct tcgacccggc cgcggatgtg gccgcgccct
    10801 tcatcgccaa gggcgcgcga ccacgcatcg cgatcctgcg cgagcagggc gtcaacggcc
    10861 aggtcgagat ggcggccgcg ttcacccgcg ccgggttcga tgccgtggac gtgcacatga
    10921 gcgacctcgt cgcgggccgg caccatctcg cggatttcac gggcttcgcc gcctgcggcg
    10981 gattctcgta tggtgacgtg ctcggcgccg gccgtggctg ggccgcgtcg atcctctacc
    11041 acggcgatct gcgcgaccag ttcgccgcgt tcttcgccga cgagtcgaag ttcgcggtcg
    11101 gcatgtgcaa cggctgccag atgctctcgc acctgaagga gatcatcccg ggcgccgaac
    11161 actggccgac cttcgcgcgc aacgcctcgg aacagtacga agcacgcttc gtgacgctgg
    11221 aagtactgga taccgagtcc gtcgtcctgc gcggcatggc cggttcgcgg atcccggtgg
    11281 tcacggccca cggcgaaggc cgggcagtgt tcgccgacga ccacaaggcg ggcaaggccg
    11341 acgcctgcgt ccgtttcatc gacaaccgcg gcaagccgac cgaggcattc ccctgcaact
    11401 ccaacggctc gccggagggg ctcgcgggat tcaccgccgc gcggggccgc gtcaccatcc
    11461 tgatgccgca ccccgagcgc gtgttccgca ccgcgcagat gagctggcac ccgcgcgaat
    11521 ggggcgagga ttcgccttgg atgcgcctgt tccggaacgc gcgggtgttc gtgggctgaa
    11581 gccggccgcc cggcgcccgc cacgatcagg gcggcgcgag ctgttccaat ggctcgtgat
    11641 cctcggcgcg cgcaccatcc tcgtcgagtc gtggtcgatc gtgccgcgca aacccggcgc
    11701 gcatgtcgca cccgctcggc tgctggaaca gcatcaagcc gaactcgggc aggatcgtga
    11761 tcaggtggtc gaagatgtcg ccctgcacat tctcgtgcgg cacgaagctg gtgttgcgcg
    11821 tgtaggcata cacctgcagc ggaatgcccg tgggagccgg atcgagctgg cgcaccgccc
    11881 agaacaggtc ctggttgatg cccggatggc tgccgatata ggcctgcatg taggcgcgga
    11941 agctgcccag gttggtcagg cgccgctggt tgatcgcatc cgcgccttcc ctgcccaggt
    12001 cggcgttcca cgcggcaagt tcgctgctct tctgctggaa atagtcgtcg agcaaccgga
    12061 agcgctgcag gtgctcgatc tccccgggcg tgtggtagcg cacgctggtg atgtcgatgt
    12121 tgagcgcccg gcgtatgcgc cgcccggttt cgtacatgtt gcggtagttg cggtacgact
    12181 tggagatgag gtgccaggtc gggatggtgc tgagggtctt gtcccagttg accaccttca
    12241 ccgtattcag agagatctcg accacgtcgc cgtcgacatt gtcttcgggc atggagatcc
    12301 agtcgcccac gcgcagcatg tcgtaggaac cgatctgcac gctcgccacc accgacatga
    12361 tggtgtcctt gaagatcagc aacagcaccg ccgataccgc gccgaggccg gccagcaagg
    12421 tcaacggatc gcgctggaac aacgccgcga tgatcaggat cagcccgacc acgaacagga
    12481 cgatcttgag gagctgcacg taacccttga tggggcgctc gcgcccgcgc ggggtctggt
    12541 tgtagaggtc ctccatggcg gacagggccg cgtcgaccgc gagcaccacg aacacgatga
    12601 tcagtgcggt ggtgatggcg gccacgacct tttccgcggc ctcgggcacg cccggcaccg
    12661 cgtgcaggcc tgcgttgacg atcagcatcg gaaccacctg cgacagacgc aggaacacgc
    12721 gacgcttgac catggcgtcg tcccagcgcc acgtggtgtg cctgctggcg aggcgcatga
    12781 cgtgcaccat cgcgtagcgc accagccgcc ccgccagcca cgcgaccagc agcagcagaa
    12841 cgcaatcgag cgcggtgcgc acccatagct ggtcgaggaa cgattcgaac gtttgcatgg
    12901 cgaggcgtgg attgcagtga aagccccaac cataacaagg aacgcgcggc gctgcccccg
    12961 ccgcaggcca ggcgaacgcg gcaacgccca gccgtagccc tcgcccgcga cgactttgcc
    13021 gcggaacacg ctgtaggccg gcggccgacc taggcccccc accaccctgg caccagcgcg
    13081 atgcaacccg acggcgccat gaacctccac gcgcggtgcg aaagccacat gaaaaacgga
    13141 cgagcgccga tcaggcgcgc tgcaatggcg cacgcaagtg cgcgcggtcg cacatccgga
    13201 tcgcgcgtgc cgcaatcagt ccagcggatc aggccattcg ttggcgtgcg ggccgatctg
    13261 gccacgctgc ggtcgcacca cgcagaaccg ctggccggtc ggcgcttcca tcacgaccca
    13321 ggtcttgatt gacccgacgc gcttggcgcc aagcgcctcc agtcgccgca cttcggcttc
    13381 gatgtcattg ctttcgatgt cgaggtgcac gcggctgtcg tggtctacct tctgcacctc
    13441 gatcaccggc tcgtcgtcgt gcgtcaccat gccgatgtag ttgcttccgc cgggattcgt
    13501 cccttcgcgc aatggccgcc ccagcgcttc ggaccagaat cgggccgccg attccaggtc
    13561 atcggtccgg caatcgatga ggatgctgca caagcggctg cggtgcatgg tggtcctcta
    13621 tttgtcgtgg gcgggtccgg ccgtcacgcg cgcctgagtg cgcggtcaag gctccatggc
    13681 cccggcccgg ccgccgacag gtacaggaac gtgaagcaga acagcaccgc caggccgccg
    13741 ccgttgagga tggggaccac gtggttgtgc gggaagtgcg ccatgaaata cgcgaacgcc
    13801 atttcgcccg acaggatgaa ggcgaccggt cgcgagaaca gcccgatcaa cagcaggaaa
    13861 ccgccgccga actccaggat gccggcgagg ccgggcacga tggtgaacag cttggccgac
    13921 gagatcagct cgaatgatcc gccgggataa ccgaacagct tttccgcccc gtgctgcatc
    13981 aaaaggaatg cggcgatgat gcgcaggatg ctcagcaggt acggcgcggt ccgcgcccag
    14041 gacccgttgt tgccagccat gcggatgcct ccctccggtt gtcgtcgccc gagtgtatcc
    14101 ggaccccgcg cgcggcgggc aacccgtcca cgtggtgcgc aaggctcagt atgcgagcgc
    14161 ctggacctcg tcgatggcct tcggaagcgc agctgtcagc acttcactgc cgtgtgcgcc
    14221 gatcgcgacg tcgtcctcga tgcggatgcc gatcccgcgc cacttcgccg gaaccgtggt
    14281 gtcgtcgggc gggatgtaga tgccgggctc gacggtcacg accatgccgg cctccagcaa
    14341 cctcggctgc tcgtccacgc ggtaatcgcc gacgtcgtgg acgtccaggc ctatccagtg
    14401 cccggtcttg tgcgggaaga agcgctcgta gctgccggac ttcaatgcgc ttcgcgggct
    14461 gccggacagc agcttcagtt ggcacaggcc ctcgacgacc accttgcgcg ccgcgtcgtg
    14521 cgcggccgtg aacgccgccc cttcgcgcac ctgctcgatc gccgcctgtt gcgcagccaa
    14581 caccacctcg tacagcgcgc gctgttcgcg cgagaagcgg ccgccgacgg gccatgtgcg
    14641 cgtgatgtcg gaggcgtagc actcgacttc ggcgccggcg tcgaccaaca gcaggtcgcc
    14701 gcgctcgagc cggtcgcggt tggcggtgta gtgcagcacg caggcattgg cgccgcccgc
    14761 cacgatcggc gcatacgagg gcacggcgcc gtgcgcgcgc accacgtgca gcagcgcggc
    14821 ttcgacttcg tattcggcgg cgcccggacg cgctgccgcg accgccgcgc gctgcgcttc
    14881 ggccgcgatg ttcgcggcgc gcttcatcag gcgcagttcc gcggtcgact tgtacaggcg
    14941 caattcgtgc agcaagtgcc cgagcgcgac gaactcgcgc ggcaccacgc cgccgccgcg
    15001 cgacgcccgc aacctgcgca gccagcgcag caggtgcgcg tcgaactcgt tctcccgccc
    15061 gaaatggcag tacaggcggg tacggccctc gagcatgccc ggcaggatat cgtcgatgtc
    15121 ctccaccggg aacgcatcgt ccatgccgaa gcgcgcggcc gccgcgtcgg ggccgatgcg
    15181 cgcgccatcg atcctttcgc gctcgggatc gcgttcgcgg cagaacagca cgacctcgcc
    15241 attcgcgcga cccggcagca gcgcaagcac cgcgtcgggt tcggggaaac cggtgaggta
    15301 atggaaatcg gaatcctgcc ggtacggcca ctcggcgtcc gcgttgcgga tccgccgggg
    15361 cgccgcggcc accaccagcg ccgcatcctc gccggccatg cgcatcagtt cgcggcgcct
    15421 gcgggcgaat tccgcgggct ggatcatcgg cgaaccgccc gtgcaacgcg ggcatgcagt
    15481 tgcacggtgc cgccgcggat gaattccagt acgtcatcga gtgcgccctc gtcgccttcg
    15541 tcgcactcga tgcgcatcga tgcgatttgc ccgaaggcat ccagcagctc gcgcgtgccg
    15601 gcatccagcg ccgaaccctc cagcgcgccc gtcaagccga ggccgcccag gaagccgcgg
    15661 caccagtcca ccatgccatc ggcgcgggcg gccagcggcg cttcgggcag caagggcgcc
    15721 acggcctcgc cggcgccgag gtcgcgggac atgccggcgg ccagttcgtc gagcagcgcg
    15781 tgcagcggat cgagcgcgcc ggcgtcgtcg ctttccagtt gcagtgccga caacaacacg
    15841 tggccgtcgc cgcccccgcc cgcgcacagg tagcccgcag ccgaaccgtg cagttccgcg
    15901 gcgtcgatgc ccagcctcgc ccgcgcgacc gccttggcga attccgtgta gctgacctcc
    15961 cgcatgcgcg tcattctatt gaggctttgg gggatatggc gcgttggcgc ccgggtcgcc
    16021 cgacgcaggc cgccgatcgc cccgaaagcc gatccgggcg gactgcacaa aagcgacccc
    16081 ggccgcacga cgcgccgcac cggtttgagt aaactggggc gcgcagcctt gcccaggtcc
    16141 tccgtggaac ggtggtcgtg caacggatgg ctgcgaccac caggggccaa gtgcgtacac
    16201 catgagccgc gttcctaccg gaccctccgg gccgacaccg ccagccgcgc gctcgcacat
    16261 cctgccggcg ctggcctgcg tgctgctgtg gttcgctggc gtggcgaccg cggcccaggc
    16321 gccgccggcg acctggcagg cctaccagcg gaccttctat ttccagcagc tcgacagcgc
    16381 cgacggcctg gcgcagaact cggcgtcggt ggtgttccag gaccaccggg gcttcatgtg
    16441 gttcgcgacg cagggcggcc tgttccgcta cgacggctac aagctcaccc gctacaccca
    16501 cgaccctgat caggacgacg gcctgcccgg gggctttccg gtgtcctcgc tcgccgatgg
    16561 tcccgacggc aagctctggg tgggttcgtc cagcggcggg ctggtcctgc tcgatccgga
    16621 cggtggcacg gttgtcccgc tcccgaaagg cgtggaacgc agcgacctgc cggtcaccag
    16681 cctgctcggg ctcgcggacg gagacgtcct gatcggcagc agactcgggg tggatcgttt
    16741 ggtcgccgcg ccgcggccgc acctgcagcg cgcctgggcg gagtcgcaag ggatggccga
    16801 gcccgtgcac gcgctgacga gctgccctga cggcgcggtg ttcggcgtgg cgggcgattc
    16861 catcatcgcg ctggacgcca aggccggcca cggccggatc ctggcgacca cgcagcagcc
    16921 cgtcgatgcc ctccattgcg atgcggcgag ccaccggctc ctgctcggca accgggacgg
    16981 cctcgcgagc gtcgaccgga aatccggcgc gctcgtatgg ctctggcgca gcgacggcga
    17041 cagcgcggtc ggcgttcgcg acatcgctgc cgaccaggcc ggacgcgtgt ggctggcgtt
    17101 gagcgaccgc agcctgctcc gcctgggccc ggatggcgcg gaacaagaag tggtgcccgc
    17161 accggtgggc atcaccggcg ggcttcccga ttcgcggatc gataccctgt tcgtcgatcg
    17221 cacgggtttg ctgtgggtcg gcacgctctc ggccggggtg gtatggacgc acccggacgg
    17281 caatccgatc caggccgtgc tgaacctgag cggcgatccg cgcgcccacc attacatccg
    17341 ggggttccac gccgacggcg atggcaggta ctgggtggcg ctgagcggtc ccgggctggt
    17401 ccgctatgac cgcgccaccc acaccatcac cggtttcggc agtgcgctcg ccgaagcggt
    17461 ggcgtctgtc ccgcacgcgc cggtggccgc ctcgacctcg gccgccccgg cgacggcgat
    17521 ccgcagcttc ggcgtcattc cggattccga cgggactctg ctggttccgt ccagcgcggg
    17581 aatcctcagg ttcgatccgg ccaccggcaa ggcaagctac gcggacctcg acggcaagcc
    17641 cgccaccacg gcggtgcgcg cgctgtaccg cgcgcacaac ggcgacctgt gggtcgcgta
    17701 cgcaaaccag gggctggtgc actaccacga aggccaggcg ttgcagcgct tcgacgccga
    17761 ccacggcctc ggctcgaacc aggtgctggt actcgacgag gacagccacg gcaacatctg
    17821 ggccggcacc acccgcggcc tgtcgatgat cgagcccgcc agcggcaagc tgcggacctt
    17881 ccgggaaatc cccggccgcg acgactcgct ggcgggcagc accatctcca gccttctcgt
    17941 cgccgccgac gggcagttgt gggtcgggtc gctgtccggc atcagccgcc tccagggcat
    18001 cgacgccgct ggcgcgcatt tccagcgtta cggcctggat gaagggctgc cggacaacac
    18061 catcgactgc atgctcgagg atgccagcgg cgacatctgg ttcagcacca acctcggcat
    18121 cggcaggctc gacccgaaga ccggcgcgat ccgcaacttc ggcgcgtccg atggcctgca
    18181 aggcagcgaa tacaactcgg gcgcctgcct gcgcacgcgg gaaggccgcc tgctgttcgg
    18241 cgggcacggc ttcgacatca tcgatccggc caaggtgcgg ccctcgacga cgcaggcgcc
    18301 ggtcgtcatc accgacctgt acgacgccgg ccaccgcatg gcagtgccgg catcgtcgtc
    18361 cgcacgcatc gatatcggcc ccaaggaccg cagcctgtac atctccttcg cggcgctcga
    18421 ctacggctcg ccggggcaca gccgataccg gttccgcctg ctgggacgcg acgcccggtg
    18481 gagcgaaccc acccaggacc attcggtcgc ctactccagc ctcgcgcccg gcgattaccg
    18541 cttcgaggtg cagtcggtgc tccgcgacgg ccgcgccggg agtaccgctg ccgtcgccgt
    18601 gcacgtggcg gcgacgtggt ggtggagccc ggtgatgcag gtcgtgtacg cggcggcgat
    18661 cgtgctgctg gtcctgatcg cgatcttcgg ctggcgcagc caccaccacg agcaacagcg
    18721 gcaccgtcgc cagttgcgac tgcgcgaaag ccgccttcgc caggcgctgt ggggctcggg
    18781 cgacgaattc tgggactgga acctggagag cggcacgatc ctgcggttgg ggccggaggg
    18841 ggcgcccggc ggccaccacg aacaagcggt gccggccgac gaatggcggc gcacgatggt
    18901 gcatccggac gacctcgcca gcctcgacca tgcgctggcc gaacacctgg cgggcaacac
    18961 cgagcatttc gaagtcgagc accgggtgcg cggtcgcgac gacgaatgga cctgggtgct
    19021 gtcgcgcggc aaggtcgtgg agcgcgacgc cggcggcaag ccgctccggg tgtgcggcac
    19081 cgagcgcgac atcagcgcga tccgcgccgc cgaccgcgac cgccgcatcg cctccgaggt
    19141 catccgcaac atgagcgagg cggtcaccgt caccgacctc gatttccgct tcgtgtcggt
    19201 caacaccgcc ttcacccgca tgaccggcta tgccgagcac gaggtgctcg gccgcgacgc
    19261 ctcggtgctc aacggcagcc gccacgcacc ggaaagctac acccacctgc gccagatgct
    19321 ggccgagacg ggccattggc gcggcgaact gtggcagcgc cgcaaggacg gcgaggagtt
    19381 cctgtgctgg gtggaactca ccgaagtgtg cgacgcatcc ggacaacgca cccacatgat
    19441 cggcgtgctc accgacatca ccgaccgtaa gcgcgcggaa caggaacttc gctacctcgc
    19501 gaactacgac accctgaccg gcctgcccaa ccgcgcgctg ctcggcgagc ggctgggcgc
    19561 ggccctcatc agcgcgcggc gcagcggcca caaggtcgcg ctgctgttcc tggacctcga
    19621 ccgcttcaag cacgtcaacg attcgctcgg ccacaccgcc ggcgaccgca ccctgaaagc
    19681 cgccggcgcg cgactgcgcc agtgcgtgcg cgagaacgac gtggtcgcgc ggctgggcgg
    19741 cgacgaattc acggtggtgc tggaagacct ggcggacgtc ggcggcgccg aggaaatggc
    19801 ccagaggctg atccatgcct tcgccaagcc cctgcaggtc tcgggcaccc aggagacggt
    19861 gatctcgccg tccatcggca tcgcgatgta ccccgaccac ggccagacgc ccgcggacct
    19921 gttgaagtgc gccgacaccg cgatgtacca ggccaaatcc agcggccgca acacctgggc
    19981 ggtgtaccac gccggcatgg atgcgcgcgc ccgcgaccac gccaccctcg tcaatgccct
    20041 caggcgcgcg ctggatcgcg gcgagctgcg gctggtctac cagcccaaga tgtcgctgca
    20101 cggcgaaaac atcaccggca tggaggcgct gctgcgctgg cacaacccgg acctcggcga
    20161 gatcccgccc agcgtgttca tcccgttggc ggaggagacc ggccagatcc acgagatcag
    20221 cgcctacgtc ttcgggaccg cctgcgcgga cctgcgccgc ttccgcgaga acggccatcg
    20281 caacctcacg atggcgatca acctttccgc ggcgcagctc aatcgccccg acctgacctc
    20341 gcacatctgc gacctgctgg ccgaacacga cgtcgcgccc aaccaggtcg aactggagct
    20401 gaccgagagc atggtcatgg ccaacgccga gcagtcggtg cgcctgctcg gcgagctgaa
    20461 gtcgatcggc acgcggctcg cgatcgacga tttcggcacg ggctattcct cgctcgcgta
    20521 cctgaagcgc ctgcccatcg acaccctgaa gatcgaccag gaattcgtgg gcgacatcac
    20581 caccgatccc gacgacgcgg cgatcacctc gaccatcatc acgatggcgc attcgctgca
    20641 gctcaaggtg gtggcggagg gcgtggagac cctggagcaa ctcgcgttcc tgcgcgcgca
    20701 gcactgcgac gagatccagg gttactggtt ctcgcggccg ttgtctgccg aagcctgcct
    20761 ggacttcctg aaccggcacg cggtcgcaaa cgtggtttga tagcccgcac atgcccctca
    20821 acgtcttttg ccttatagtc ccgccatgga caatccagca cccaccgtcg aagacgaact
    20881 cgccgccctg acgcgtaccc tcgatcgggc gctggaacgc ctgcgccggc ttgccgagga
    20941 aaacgccagc ctgcgtcgcg gtcgcgacca gctcgccggc gaacgcgccg ccctgatgac
    21001 ccgcaacgag caggcgcgcg cgcgcgtcga agcgatgatc gaacgattga aggcgctcga
    21061 aagcgccggg taagcggcgc attcgcccga atcgcgcaac cgcgggtgac gccggcggcg
    21121 cacccgcatc cccagcaacg aaaacacagc agactgccat ggccgaagaa tccgttaccg
    21181 tcaacgtcaa gctgctggac cgtgaattcc tggtcggctg caagcccgac gagcgcgagg
    21241 ggctgctggc cgcggtggag cacctgaaca ggaagatgcg cgaaatccgg catagcgcgc
    21301 gcagtcccgg ctatgaccgc atcgcggtac tggcggcgct cgacatcacc catgacctgt
    21361 tgatgttgca gcgcagccag aaccggcttt ccagcactgc gggcgagcag atcgcgatgt
    21421 tgcgacgcaa gcttgaagat gcactcgaag cacccataga atgaaactgc tggcgtcccc
    21481 tgcggtgaac gacagcgcgc ccccagcaat gtgccttgtt cctaatgtac gacct
//
LOCUS       DASSNH010000283         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_905517, whole genome shotgun sequence.
ACCESSION   DASSNH010000283 DASSNH010000000
VERSION     DASSNH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905517"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFK00_10415"
     CDS             <1..1138
                     /locus_tag="VFK00_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7593403.1"
                     /translation="WLPVDQYIGGIEHAILHLLYFRFYHKLMRDAGLVHSDEPATNLL
                     CQGMVVAETFYRQQGTGNREQGAAVNSSSDSPFPVPRSRSSDQGLGPASEEQGEKEWI
                     NPADVDLLRDERGRIVGATLKADGEPVALGAIEKMSKSKNNGVDPQTMIDKYGADTVR
                     LFSMFASPPDQSLEWNEAGVEGMARFLRRFWREVVTHASQPDHRVVDRAVLDPAQKAF
                     RRQVHETIAKVGDDIGRRRNFNTAIAALMELLNAVARFGDMGDAGRALRHEALEAMVL
                     LLNPFTPHASHALWQVLGHGETLVEDVPWPKADPAALVRDTVTLAVQVNGKLRGTLEA
                     APDVPREHLEAEARALPGVVAAMGGKSVRKVIVVPGKIVNIVAG"
     gene            1436..1879
                     /gene="lptE"
                     /locus_tag="VFK00_10420"
     CDS             1436..1879
                     /gene="lptE"
                     /locus_tag="VFK00_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HET7593404.1"
                     /translation="MAQRVYLKVNGGGEFPRSLAAALRASKIEVLDEPAEGVATLSVP
                     VADFSSNLLTTSGFQRVGEYVVGFRVRFQLTDGNGNTVVPAQTISLSHEFAVDRTQFS
                     AISSETEAIQRSLVREMTDALMRRLEARAAHPLPPGEPASAGSAG"
     gene            1883..2911
                     /gene="holA"
                     /locus_tag="VFK00_10425"
     CDS             1883..2911
                     /gene="holA"
                     /locus_tag="VFK00_10425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7593405.1"
                     /translation="MAGELADFLRTLSKGDLEPVYLLGGEEPLQLQEAGDAVRARARE
                     LGFTEREVLDVETHFDWDRLARSGASLSLFASRRLIELRLPTGKPDRDGAAAITEWCK
                     APAPDTVLLISAMQWSRKHEGAWVKAVERAGGYVRLQAPRVDQLPAWITARMRRHGFV
                     PTDDAVALLASRAEGNLLAIAQEIDKLVVLRGAGPVDAEELESLGDDNAIYDVFRLTD
                     AAFGGDGPRALRVLAGLRAEGEDVIPMLGWLLTQLDLALRLASAANFAEAARAEYNFW
                     PARQRLFAGALKRAGSGDAHWRRCLAWCARIDRMSKGRETGDPWRELERLLVAIAQPR
                     SAAALLAA"
     gene            3017..3745
                     /gene="nadD"
                     /locus_tag="VFK00_10430"
     CDS             3017..3745
                     /gene="nadD"
                     /locus_tag="VFK00_10430"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7593406.1"
                     /translation="MNPERPLAIFGGTFDPVHIGHLRAAWEAAGMLDAEVRLVPARVP
                     PHRPQPVASAEQRAAMLRAALAGQDRLALDLRELDRQGPSFTFDTLAGLRGEVGASRP
                     LVLLIGADAFAGLASWHRWRELFGLAHMCVLTRPGQIPAMTEDLAGEVAARLVDDVGD
                     LCSSPAGHVLNMIVTSLGISATRIRDELAHGGDPRWLVPQALLDDPALLEPYRAKREA
                     GTGNREPGSTAKDASPFPVPRSRS"
     gene            3833..>4120
                     /gene="rsfS"
                     /locus_tag="VFK00_10435"
     CDS             3833..>4120
                     /gene="rsfS"
                     /locus_tag="VFK00_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7593407.1"
                     /translation="MTSQTESRPAATPVTRPPSFATLRRRVLAALDELKAHDIREIDV
                     RGRTSIADLLIIASGTSSRHVKSIADEVVKFAKRAGMLPLGVEGEDEAEWVL"
BASE COUNT          660 a         1363 c         1434 g          663 t
ORIGIN      
        1 ctggctgccg gtcgaccagt acatcggcgg catcgagcac gcgatcctgc acctcctgta
       61 tttccgcttc tatcacaagc tgatgcgcga cgcggggctg gtgcattcgg acgaacccgc
      121 gaccaacctg ttgtgccagg gaatggttgt ggcggagacg ttttatcgac agcagggaac
      181 agggaacagg gaacagggag cagcggtcaa cagctcttcc gattccccgt tccccgttcc
      241 ccgttcccgc tcttccgatc aagggctggg accagcaagc gaggagcagg gcgaaaagga
      301 gtggatcaac ccggccgacg tcgacctcct gcgcgacgag cggggacgga tcgtcggcgc
      361 gacgctgaag gcggatggtg aaccggtcgc cctcggggcg atcgagaaga tgtccaaatc
      421 gaagaacaac ggcgtcgatc cgcagacgat gatcgacaag tacggcgccg atacggtgcg
      481 gctgttctcg atgttcgcat cgccgcccga ccagtcgctg gagtggaacg aagctggcgt
      541 cgagggcatg gcgcgcttcc tgcgccggtt ctggcgtgaa gtcgtgaccc atgcgtcgca
      601 gcctgaccat cgggtcgtcg atcgtgcggt gctggatccc gcgcaaaagg catttcggcg
      661 gcaagtccac gagaccatcg ccaaggtcgg cgacgacatc ggccggcggc gcaatttcaa
      721 caccgcgatc gccgcgctga tggaactgct gaacgcggtc gccaggttcg gcgacatggg
      781 cgatgccggg cgcgcgctgc ggcacgaggc gctggaggcc atggtgctgc tgctgaaccc
      841 gttcacgccg cacgcaagcc atgcgctgtg gcaggtgctc ggccatggcg aaacgcttgt
      901 cgaggacgtg ccctggccga aggccgatcc ggccgcgctg gttcgcgaca cggtgacgct
      961 ggcggtgcag gtgaacggca agctgcgggg cacactggaa gccgcgcccg atgtgccgcg
     1021 cgaacatctc gaagccgagg cgcgtgcgct gccgggcgtg gtcgcggcga tgggcggcaa
     1081 gagcgtgcgc aaggtgatcg tggtgccggg caagatcgtc aacatcgtcg cgggctgagg
     1141 gtgtcccgtg atgccgcggt ggcactgtta tccttcgcgg attcgggacc cggggcaatt
     1201 gcacattcct cctggtcctc atgccgggtt cgattgccaa gggagccggc gagcgccgga
     1261 atgacgactt cgtgaagttg cggccgcgtc cggaaccgta atccggatcc atcgtgcaac
     1321 cggagctgat tcgatgcgga tccaaaagtg gctcgtcttg ctggcgtgtg tcgccgccgc
     1381 cgcccttgcc ggttgcgggt tccatcttcg tcgcagcgcc gctctgcctg cgccgatggc
     1441 gcagcgcgtt tacctgaagg tgaacggtgg cggggaattc cctcgctcgt tggcggccgc
     1501 gttgcgggca agcaagatcg aagtcctcga cgaaccggcg gaaggcgtcg cgaccttgtc
     1561 ggtgccggtg gcggatttct cctccaacct gttgaccacc agtggtttcc agcgggtggg
     1621 tgaatacgtg gttggctttc gcgtgcggtt ccagttgacg gacggcaacg gcaacacggt
     1681 ggtgcccgcg cagaccatca gcctgtccca cgaattcgcg gtcgaccgga cgcagttctc
     1741 cgcgatctcg agcgagaccg aggccatcca gcgcagcctg gtgcgcgaaa tgaccgacgc
     1801 gctgatgcga cggctcgagg cccgggcggc gcacccgctg ccgcccggcg aacccgcttc
     1861 cgccggcagc gccggctgac ccgtggcggg cgaactcgct gacttcctgc ggaccctctc
     1921 gaagggcgac ctggagccgg tgtatctcct gggcggcgag gagccgctgc aattgcagga
     1981 ggcaggcgac gcggtgcgtg cccgcgcacg agaactcggc ttcaccgagc gcgaggtgct
     2041 ggatgtcgaa acgcacttcg actgggaccg gctggcgcgc agcggcgcga gcctttccct
     2101 gttcgcgtcg cgccggttga tcgaactgcg cctgccaacc ggcaagcccg atcgcgacgg
     2161 cgcggccgcg atcacggagt ggtgcaaggc acccgccccc gacaccgtgc tgctgatcag
     2221 cgcaatgcag tggagcagga agcacgaagg cgcgtgggtg aaggccgtgg agcgggccgg
     2281 cggatacgtg cggctgcagg cgccgcgtgt cgaccaactg ccggcctgga tcaccgcccg
     2341 gatgcgcagg cacggcttcg tgccgaccga tgacgccgtc gcgctgctcg cgtcgcgcgc
     2401 cgagggcaac ctgctcgcga tcgcgcaaga gatcgacaag ctcgtggtgc tgcgtggggc
     2461 cgggccggtg gatgcggaag agctggaatc gctcggcgac gacaatgcga tctacgacgt
     2521 gttccgcctc accgacgcgg cattcggcgg cgacggaccg cgcgcgctgc gggtgctcgc
     2581 ggggctgcgc gcggagggcg aggacgtgat cccgatgctc ggctggctgc tgacccagct
     2641 cgacctggcg ttgcgcctcg cttcggccgc gaacttcgcc gaggccgcgc gcgccgagta
     2701 caacttctgg ccggcgcggc agcggctgtt cgcgggggca ttgaagcgag ccggcagcgg
     2761 cgatgcgcat tggcgacgct gcctcgcctg gtgcgcgcgg atcgatcgca tgagcaaggg
     2821 ccgcgaaacc ggcgacccgt ggcgcgagct cgagcgcctg ctggtcgcga tcgcgcagcc
     2881 gcgcagcgcc gctgcgttgc tggccgcgtg aaaaagcaac cccgtccgca aatgatcgcg
     2941 aaacaaccaa cagggggctc agccatctgc gttcattcgc attcgttccc cgacagcatc
     3001 gccctggatg tccaacgtga accctgaacg cccgttggcg atcttcggag gcaccttcga
     3061 tcccgtgcac atcggtcacc tgcgcgcggc gtgggaagcc gccggaatgc tcgacgccga
     3121 ggtgcggctg gtgcccgcgc gcgttccgcc gcaccgacca caaccggtcg cgagcgccga
     3181 gcagcgggcg gccatgttgc gggccgcatt ggccgggcag gatcgcctgg cgctggacct
     3241 gcgtgaactc gaccgccagg ggccatcgtt caccttcgac accttggccg gtttgcgcgg
     3301 ggaggtcggc gcatcgcgac cgctggtgtt gctgatcggt gccgatgctt tcgcggggct
     3361 cgcgagctgg caccgctggc gcgaactgtt cgggctggcg cacatgtgcg tcctgacccg
     3421 gcccggacag atcccggcga tgaccgagga tctcgccggc gaagtggccg cgcgcctcgt
     3481 cgacgacgtg ggcgacctgt gttcgtcgcc cgccggccac gtcctgaaca tgatcgtgac
     3541 ttcgctcggc atttcagcga cgcgcatccg cgacgaactc gcccatggtg gcgacccgcg
     3601 ctggctggtg ccacaggcgc tgctggacga cccggcgttg ctggagcctt accgtgcgaa
     3661 gcgtgaagcg ggaacaggga acagggaacc gggatccacg gcgaaggatg cttccccgtt
     3721 ccccgttccc cgttcccgct cttgaatcgg ccacacttgc gctcgaatcc gagtggtgca
     3781 tactggacct gcgcaatccg cgcgccatct cgaggccgag gccttccaca acttgaccag
     3841 ccagaccgaa tcgcgccctg ccgcgacccc cgtcacccgt cctcccagct ttgcgaccct
     3901 gcgccggcgt gtcctcgccg ccctcgacga actgaaagcg cacgacatcc gcgaaatcga
     3961 cgtgcgcggt cgcacctcta tcgccgacct gctgatcatc gcctccggca cctcctcccg
     4021 ccacgtcaaa tcgatcgccg acgaagtcgt caagttcgcc aagcgcgcgg gcatgttgcc
     4081 gctcggcgtc gagggcgagg acgaagccga atgggtgctg
//
LOCUS       DASSNH010000284         6207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1544524, whole genome shotgun sequence.
ACCESSION   DASSNH010000284 DASSNH010000000
VERSION     DASSNH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544524"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFK00_10440"
     CDS             complement(<1..272)
                     /locus_tag="VFK00_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593408.1"
                     /translation="MRFRGRDELAGDLCHWRFSLEAANSAGSVRRRRGGRLEGIQLMV
                     SIAFASVRRARSHPPRMMVIAAVAVLTMLAGLADAHAGIGRGDRYAG"
     gene            733..1251
                     /locus_tag="VFK00_10445"
     CDS             733..1251
                     /locus_tag="VFK00_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET7593409.1"
                     /translation="MYSRIFRTTALATALAFSSLAFAAGAAAAQSTPSSPAAQPGAPA
                     KGQSLLEELDLTQSQQSSIRTTMQQNFQQLRPQMQSVTQKRQAFEDATPGTSAFQSAM
                     NDLAQAEADFAKARTLREGALRSKIYEVLTPTQRTKLKDLIAQQKAQVEKMREAARAR
                     RASGSSTPPASH"
     gene            1315..1971
                     /locus_tag="VFK00_10450"
     CDS             1315..1971
                     /locus_tag="VFK00_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593410.1"
                     /translation="MPAIRTLLRIFLAIAIAASLAACNPDHDAGSHQPQASEALPSRT
                     ADAGVHRWRDTTWQVSIDVPPGWTIHHDFSASYLANGAWKTYAGPDSQGSSIVALVAS
                     GSNHVTDAEIRIGASRAATEVRHCATPPDAVRPGSLSHETVGGIDFTTFEAADAAMSH
                     YLDVHSYRAVRRGACYAIDLLVYGANPQVFDPPVTPPFSRAEAFARMRSVLASFRLTH
                     "
     gene            2094..2687
                     /locus_tag="VFK00_10455"
     CDS             2094..2687
                     /locus_tag="VFK00_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET7593411.1"
                     /translation="MHTKVLRSALLAAFAGFSSLAFATAGAQTADTPTSASSAPMHRG
                     MHHGMQRWHGSQQEHMDVLRQLDLTDTQRSHIQQMMRDEHTQSRPEMQALMQKRKALE
                     NATPGTAEYQTAAQQLGQAEAQAAQARVTRHAQMRAKIYNELTPEQRSKLASLSSEHQ
                     DRMQDWRSKRAQRPERAQRAVNEGAEAAPASTSGGQP"
     gene            complement(2743..4158)
                     /locus_tag="VFK00_10460"
     CDS             complement(2743..4158)
                     /locus_tag="VFK00_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET7593412.1"
                     /translation="MLLAEYLWTAAGWQRDARIEGAPGELRARFVLPGMPNLHSHAFQ
                     RAMAGLAERRGPDEDSFWTWRETMYAFASRIGPDELRAIAAQLYVEMLKAGYTHVCEF
                     HYLHNAPDGRPYADPAAMSQAIIEAAGEAGIGLTLLPVLYMTGGFDGRPLSERQRRFG
                     ITVDAYLRLVESLSLQESPQVHAGIALHSLRAVPESAMREVLGCLPSSSPLPPGEGAP
                     EGRVRVRPEQGETSSSEPPPRRGGRGFMPIHIHIAEQIGEVQDCLGVRGARPVQWLFD
                     HADVDARWCLVHATHLDDAEMQAIARSGAVAGLCPTTEANLGDGLFPLAGYLGAGGAF
                     GIGSDSHISVSPVEELRWLEYGQRLVTRRRNIAARRSGDSGGQTLWRAALHGGRQAAG
                     LPGSGGLVGDGLIVLDDGSPLLAARGERDVLDAFLFAGNIPLVRDVMVDGQWVVHDFR
                     HRDEERIAARYRDAVQHLAGD"
     gene            4265..5824
                     /locus_tag="VFK00_10465"
     CDS             4265..5824
                     /locus_tag="VFK00_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HET7593413.1"
                     /translation="MKPAHEHTARLAVRNTREEDIPQVAALGKRVYGPGFGYSPEMLA
                     GQLAHFPQGQFVAELDDRIIGYCATFRISAALAMKPHTWDEITGSGFASRHDPDGEWL
                     YGMDMGVDPDRRGMRIGRRLYGARKRLCQRLRLCGIVFGGRLPGLAKNVKKYPTPEDY
                     LKAVVEGRQRDPTLSFQLRNGFEPIGLLTDYLPYDRDSLGYAAHLVWRNPRRMDQPDV
                     PSHPATQRLPDVIRLATVQYGQRRVRDFDEFATQVGYFVDIAANYDADFVVFPELLTL
                     QLLSIENAPLSPVDAILRLSEYTPRVKALFSDLAVRRNINIIAGTHPTRAEDGAIHNI
                     CYVCLRNGSVHQQVKLHPTPNERNWWGISGGSIARAIPTDCGPIGVTVCYDTEFPEVA
                     RHLIDQGALILFTPFCTDTREGYLRVRYCAQARAIENQCYVVLSGNCGNLPGVNNFDI
                     QYAQSCILTPCDFPFSRDGIAADSTPNSETVLFADLRLEDLARARNNGTVQNLKDRRH
                     DLYHLRWPARS"
     gene            5821..>6207
                     /locus_tag="VFK00_10470"
     CDS             5821..>6207
                     /locus_tag="VFK00_10470"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974230.1"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET7593414.1"
                     /translation="MTADRDLSAGERWDHLFLDCTLATLSGDSGYGLVDAGAIGWRNG
                     AITYTGPTDALPGTPDALASQVESARGALVTPGLVDCHTHLVFGGDRADEFEMRLNGK
                     SYEAIARAGGGIVSSVRATRSASEERL"
BASE COUNT         1103 a         2197 c         1952 g          955 t
ORIGIN      
        1 ccggcgtaac gatcgccgcg gccgatcccg gcatgggcgt cggcaaggcc ggccagcatc
       61 gtgagtacag cgacggctgc gatgaccatc atgcgcggcg gatggctgcg cgcgcggcgg
      121 accgatgcaa aagcaatcga aaccatgagc tggatcccct ccaggcgtcc gccacgtcgt
      181 cggcggaccg aaccggcact gttagcagcc tcgagcgaaa atcgccagtg gcaaaggtcg
      241 ccagccagtt catcgcgccc gcgaaagcgc acttttgcag gttggcgctg agcgcagcga
      301 agcccaacaa tcgccccggc tcagcgcact ccccacccgc cgccctgtcc tctcccgacg
      361 tgtgttgttg ggcttctccc ggagcaagtc cgggatcaac ccaacctaca acagccgctt
      421 ctgtaggttg gcgctgagcg cagcgaagcc caacaatcgc ccagccatag tgttcggcaa
      481 aacccgctct tgcaggttgg cgctgagcgc agcgaagccc gtcaagcgcc cagttcatcg
      541 ttcggcaaag ctgcttttgt aggttggcgc tgagcgcagc gaagcccaac atcgtgccca
      601 ctcgccaggg cgagaacccg agcgtccgcg catgcgaacg ctgtatcggc gccgcgaact
      661 catgtatttt ctgctgcacg cgtgccgcgg agccgctacg gttcgggacg cagccattca
      721 aggagtccac cgatgtacag caggatcttc cgcaccactg ccctcgccac cgcccttgca
      781 ttctcttcgc tggctttcgc cgccggcgct gcggccgcgc aatcgacgcc gtccagtccc
      841 gctgcgcaac ccggcgcgcc ggccaagggg caatccctcc tggaagaact cgatctgacg
      901 cagtcgcagc agtcgagcat ccggacgacc atgcagcaga acttccagca gctccgtccg
      961 cagatgcagt cggtgaccca gaagcgccag gccttcgaag acgcgacgcc cggcacttcg
     1021 gcattccagt cggccatgaa tgacctggcc caggcggagg ccgatttcgc gaaggcccgc
     1081 acgcttcgcg aaggcgcgtt gcgcagcaag atctacgagg tgctgacgcc gacgcagcgc
     1141 accaagctca aggacctgat tgcgcagcag aaggcccaag tcgagaagat gcgcgaagca
     1201 gcgcgggccc gccgcgccag cggcagctcc acgccacccg cctcgcattg agccacgcct
     1261 gatcgcgcgt tgcgccacga tgatccgtcg tgacgcagcg cggcccgttg cggaatgcct
     1321 gcaatccgga ccctgctccg catcttcctg gcgattgcga tcgccgcgtc gctcgcggcg
     1381 tgcaatccgg atcacgatgc aggctcgcac cagccccagg catccgaagc gttgccgtcc
     1441 cgaactgcgg atgccggagt ccatcgctgg cgcgatacca cctggcaggt ctcgatcgac
     1501 gtgccaccgg gctggacgat ccaccacgat ttttcggcga gctatctcgc caatggcgcg
     1561 tggaaaacct acgccggtcc cgactcgcaa ggcagttcga tcgtggcgct ggttgcgtcc
     1621 ggctccaacc acgttaccga cgcggagatc aggatcggcg ccagcagggc cgcgaccgaa
     1681 gtccggcact gcgcgacgcc gccggatgcc gtacggcccg gcagcctctc ccatgaaacc
     1741 gtcggcggga tcgatttcac gaccttcgag gcggccgacg ccgcgatgag ccactatctc
     1801 gacgtgcaca gctaccgagc ggtacgccgc ggcgcctgct atgcaatcga cctgctggtg
     1861 tacggcgcca atccgcaggt cttcgatcca cccgtcacgc caccgttctc ccgcgcggag
     1921 gcgttcgcac gaatgcgcag cgtgctcgcg tctttccgcc ttacccattg atgccgcgcg
     1981 ggggccggga agctgaccgg tggctgtatc gcccagcgga cttcacgggt tcttagcgcc
     2041 catgcgcttg cgtaacggta acgtacctgc gttcccccac caaggctccc accatgcaca
     2101 ccaaggttct gcggtccgcg ctgcttgccg ctttcgcggg tttttcctcg ctcgccttcg
     2161 cgaccgccgg cgcccaaacc gccgacacgc ccacttcggc gtcctccgcc ccgatgcatc
     2221 ggggcatgca tcacggcatg caacgctggc acggcagcca gcaagaacac atggacgttc
     2281 ttcgccagct cgacctcacg gacacccagc gcagccacat ccagcagatg atgcgcgacg
     2341 aacacaccca gtcgcggccg gaaatgcagg cactgatgca gaagcggaaa gcgttggaaa
     2401 acgccacgcc cggaaccgcc gaataccaga ccgctgccca gcagctcggg caggccgaag
     2461 cccaggcggc gcaggcacgc gtaacacggc acgcgcaaat gcgggccaaa atctacaacg
     2521 aactgacgcc cgagcagcgc agcaagctcg ccagcctgag cagcgaacac caggaccgga
     2581 tgcaggactg gcgcagcaag cgcgcgcagc gtcccgagcg tgcccagcgc gccgtgaacg
     2641 agggcgccga agccgcgccg gcctcgacca gcggcgggca accctgatgc ttgccatccg
     2701 tgtgacgcac ggcggccgcc ctgggcggcc gtctttttgg ggtcaatcgc cagcgagatg
     2761 ctgcaccgcg tccctgtagc gcgcggcgat gcgctcctcg tcgcggtgcc ggaaatcatg
     2821 taccacccat tgcccgtcca ccatcacgtc gcgcaccagc gggatgttgc cggcgaacag
     2881 gaaggcgtcg agcacgtcgc gctccccgcg tgccgcgagc aagggcgagc cgtcgtccag
     2941 cacgatcagt ccgtcgccca cgaggccccc ggatcccggc aagccggcgg cctgcctgcc
     3001 gccgtgcaat gccgctcgcc atagtgtttg gccaccgctg tctccggagc ggcgcgccgc
     3061 gatgttccgg cggcgcgtca cgagccgctg gccgtattcg agccagcgca attcctcgac
     3121 cggcgatacc gagatgtggg aatcggaccc gatgccgaac gcgccgcccg cgccgaggta
     3181 gcccgccagc ggaaacaggc cgtctccgag gttcgcttcg gtggtcgggc acagcccggc
     3241 cacggcgccc gatcgcgcga tcgcctgcat ttccgcgtcg tccaggtgcg tcgcgtgcac
     3301 gaggcaccag cgcgcatcga catcggcatg gtcgaacaac cattgcacgg gccgtgcccc
     3361 gcgaacgccc aggcaatcct gcacctcgcc gatctgttct gcgatgtgga tgtggatagg
     3421 catgaagccc ctgcccccgc gacgcggtgg gggttcggat gaactcgttt caccctgctc
     3481 cggtcgtacc ctcacccgcc cctccggggc accctctccc ggagggagag gggaagaaga
     3541 cggcaagcac ccgagcactt cgcgcatcgc cgattccggc accgcacgca gcgaatgcag
     3601 ggcgatcccg gcgtgcacct gcggcgattc ttgcaaagac agcgattcga ccaaccgcag
     3661 ataggcgtct acggtgatgc cgaacctgcg ttggcgttcc gacaagggcc gcccatcgaa
     3721 gccacctgtc atgtacagga ccggcaacag cgtgaggccg atgccggctt cgcctgcggc
     3781 ctcgatgatc gcctgcgaca tcgcggccgg atccgcgtag ggcctgccgt ccggcgcgtt
     3841 gtggaggtag tggaattcgc aaacgtgcgt gtaacctgcc ttgagcatct cgacgtacag
     3901 ctgcgccgcg atcgcgcgca gctcatccgg tccgatgcgc gatgcgaatg cgtacatcgt
     3961 ttcacgccag gtccagaacg aatcttcatc gggcccgcgg cgctcggcga gtcccgccat
     4021 ggcgcgctgg aacgcatgcg aatgcaggtt gggcattccc ggcagcacga agcgcgcgcg
     4081 cagctcgccg ggcgcgccct cgatccgcgc gtctcgctgc cagccagcgg cggtccacag
     4141 gtattccgcg agcaacatcg tcgcttcctt cggcagagtg ggtctccggc gaaggctaac
     4201 ggcgggccaa cggcggcgcc agcatcgatg gcggacgggc acgcggcgcg gccataatgt
     4261 ccaggtgaag cccgcacacg aacacaccgc gcgcctggcc gtacgcaaca cccgcgagga
     4321 agacattccg caagtcgccg ccctcgggaa gcgagtgtat ggtcccgggt tcggctattc
     4381 gccggagatg ctggccggtc agctcgcgca tttcccgcaa ggccagttcg tcgccgaact
     4441 ggacgatcgc atcatcggct actgcgcgac cttccggatc agcgcggcgc tggcgatgaa
     4501 gccgcacacc tgggacgaaa tcaccggcag cggctttgcg tcacgccacg atcccgacgg
     4561 cgagtggttg tacgggatgg acatgggcgt cgatcccgat cgccgcggca tgcggatcgg
     4621 ccgccgcctg tatggcgcgc gcaaacggct gtgccagcga ctgcggttgt gcggaatcgt
     4681 gttcggcggg cgcctgccgg gcctggcgaa gaacgtcaag aagtacccga caccggagga
     4741 ttacctcaag gcggtggtcg agggccgcca gcgcgacccg accctgagct tccagttgcg
     4801 caacggcttc gaaccgatcg gcctgctcac ggactacctg ccctacgatc gcgattcgct
     4861 gggctatgcg gcacacctgg tatggcgcaa tccgcggcgg atggaccagc cggacgtgcc
     4921 ttcgcatccc gcgacgcagc gactgccgga cgtgatccgc ctcgcgaccg tccagtacgg
     4981 gcagcggcgc gtccgcgact tcgacgagtt cgcgacgcaa gtgggctatt tcgtcgacat
     5041 cgccgccaat tacgatgcgg acttcgtggt gttcccggaa ctgctcacgc tgcaattgct
     5101 ctcgatcgag aacgcgccgc tgtcgccggt ggacgcgatc ctgcgattga gcgaatacac
     5161 accgcgggtg aaggcgctct tcagcgacct cgcggtgcgc cggaacatca acatcatcgc
     5221 gggcacccat ccgacccgcg ccgaagacgg tgcgatccac aacatctgct acgtatgcct
     5281 gcgcaatggt tccgtccacc agcaggtcaa gctgcacccg acccccaacg agcggaactg
     5341 gtggggcatc tcgggcggaa gcatcgcccg cgccatcccg accgactgcg gccccatcgg
     5401 cgtcaccgtt tgctacgaca cggaattccc ggaggtcgcg cggcacctca tcgaccaggg
     5461 tgcattgatc ctgttcacgc cgttctgcac cgacacccgc gaaggctatc tgcgcgtgcg
     5521 ctactgtgcg caggcccgcg cgatcgagaa ccagtgctac gtcgtactgt cgggcaactg
     5581 cggcaacctg cccggcgtca acaacttcga catccagtat gcgcagagct gcatcctgac
     5641 gccctgcgat tttccgttct cgcgcgacgg catcgcggcg gattcgacgc cgaacagcga
     5701 gaccgtgttg ttcgctgacc tgcggctgga ggatttggcg cgcgcgcgca acaacggcac
     5761 cgtgcagaac ctcaaggatc gacgacacga tttgtaccac ctgcgctggc ccgcgcggtc
     5821 atgaccgccg accgggacct cagcgccggg gaacgctggg accacctttt cctcgattgc
     5881 acgctcgcga cgctatcggg cgattcaggc tatgggctgg tcgacgccgg tgcaatcggc
     5941 tggcgcaatg gcgcgatcac gtatacaggc ccgaccgatg cgctgccggg cacgcccgac
     6001 gcgctcgcgt cgcaggtcga atctgcgcgc ggggcactgg tcacgccggg cctagtcgac
     6061 tgccacacgc acctcgtgtt cggcggcgac cgcgccgacg agttcgagat gcggctcaac
     6121 ggcaaaagct acgaggccat cgcacgcgcc ggcggcggca tcgtgtccag cgtgcgcgcg
     6181 acgcgttccg catcggaaga acggctt
//
LOCUS       DASSNH010000285         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_419594, whole genome shotgun sequence.
ACCESSION   DASSNH010000285 DASSNH010000000
VERSION     DASSNH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419594"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFK00_10475"
     CDS             complement(<1..247)
                     /locus_tag="VFK00_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7593415.1"
                     /translation="MGGSEKNLVQAENISKHLHGPAGRIDILSGVSLELTAGDSLAIV
                     GASGSGKTTLLGLLAGLDVPSSGRVMLAGQELTSLDEE"
     gene            246..920
                     /locus_tag="VFK00_10480"
     CDS             246..920
                     /locus_tag="VFK00_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HET7593416.1"
                     /translation="MRAFLFMLLCCVAARAGAAPPPTFLVLGDSLSAAHNIPIDAGWP
                     HLLKARLETVAPPRRLVNASISGETTTSGRARLPDLLRQYQPALVILELGANDGLQGM
                     PLQQIRDNLDAMVGACQDAGAKVLLLGIELPLNYGPRYRDGLRAIYADLAGKYRTGLV
                     PFLLQGVALDPRLMQADGMHPTAAGERIVFDNVWHALAPMLGDRPSSTGSVESGSATP
                     RAHAAH"
     gene            984..1060
                     /locus_tag="VFK00_10485"
     tRNA            984..1060
                     /locus_tag="VFK00_10485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1018..1020,aa:Pro,seq:tgg)
     gene            complement(1261..1337)
                     /locus_tag="VFK00_10490"
     tRNA            complement(1261..1337)
                     /locus_tag="VFK00_10490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1301..1303),aa:Arg,seq:tct)
     gene            1550..2167
                     /locus_tag="VFK00_10495"
     CDS             1550..2167
                     /locus_tag="VFK00_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7593417.1"
                     /translation="MRFIGALVVWIIIGIIAYFVVAYTGAYAVGADVPHWGVTKHAIG
                     FLREHTIEKRAADIKPPPLDDPALVREGAEHYAQMCTGCHLAPGITESELRDGLYPRP
                     PNLTRFAPDPAESFWIIKHGLKMTAMPAWGKTHDDQKIWAMVAFLQKQPKMSATEYRG
                     LTANAGAMHDMQPAPAPTGTAMPPPAMSAAPAPAGAGTSPAPDHG"
BASE COUNT          414 a          815 c          818 g          382 t
ORIGIN      
        1 cctcttcatc gagagatgtc aattcctgtc ccgcgagcat cacccgaccg ctgctcggca
       61 catcgagacc ggcgaggagg ccgagcagtg tggtcttgcc cgaaccggag gcacccacga
      121 tcgcaaggct gtcgccggcc gtgagttcga gcgaaacgcc gctcaggatg tcgatgcggc
      181 cggcggggcc gtggagatgc ttgctgatat tctcggcctg caccaggttt ttttcggagc
      241 cgcccatgcg cgctttcctt ttcatgttgt tgtgctgcgt ggctgcacgc gcaggggccg
      301 cgccgccgcc gaccttcctg gtgttgggcg attcgctgtc ggccgcccac aatatcccga
      361 tcgacgccgg ttggccgcac ctgctgaagg cgcggctcga aacagtggcg ccgccgcggc
      421 ggttggtcaa cgcaagcatc agcggcgaaa ccacgacgag cggccgcgcg cgcctgccgg
      481 acctgctgcg acaataccag ccggcgctgg tgatccttga attgggcgcc aacgacggac
      541 tgcaaggcat gccgctccag cagatccgcg acaacctcga tgccatggtc ggggcgtgcc
      601 aggatgccgg cgcgaaggtg ctgctgctcg gcatcgagct gccgctcaat tatggcccgc
      661 gctaccgcga cggcctgcgc gccatctatg ccgacctcgc cggcaagtac cgcaccggat
      721 tggtgccgtt cctgttgcag ggcgtcgcac tggacccccg tttgatgcag gcggacggca
      781 tgcatccgac cgctgccggc gagcgcatcg tgttcgacaa cgtgtggcat gcgttggcgc
      841 ccatgctggg agaccgcccg tcatccacgg gatccgtcga aagcgggagc gccacgccgc
      901 gcgcgcacgc cgcgcattga ccgctgccgc ggcgcgaacg taccatggca ggtcggccgt
      961 gggtgccgcc gcgcggctcg ttccggggta tagctcagtc tggtagagcg ccagctttgg
     1021 gagctggatg tcgggggttc gaatccctct gccccgacca atgaggtgga tgagaactgg
     1081 cgccttcgtc agagggcgcc gttcgactgc ggagccatgg atggcggagc gaacagcgcg
     1141 cagcgcgcga tggcccgaag ggcgagcgcc aggaaggcgc gagtcatccc tctgccccga
     1201 ccgagttctt gcgatcatcc atgatcgctg caccgcgcga gcgccggcgc ccgatgaaat
     1261 tggcgcgcct gactggactc gaaccagcaa ccgtcggctt agaaggccga tgctctatcc
     1321 gattgagcta caggcgcgtc gctgggcatt cttgcaagaa accgtccctg gtgcgactgc
     1381 caggtcggct gtcgtccaac cccaacccga atggcgacga ctgcgcggca gtggttgcga
     1441 gccgccagtg cgcgcccgcg atgcgcgggc atgccccgcg ctgcaccgat ctgctggccg
     1501 gagtaggatt ccactcgatt aaaccgtcgc cgacgctcgg gagatcgcca tgcggttcat
     1561 cggtgcactg gtggtatgga tcatcatcgg gatcatcgcg tatttcgtgg tggcgtacac
     1621 cggcgcgtac gcggtgggtg cggacgtccc gcattggggc gtcaccaaac acgcgatcgg
     1681 tttcctgcgc gaacacacga tcgagaagag ggcggcggac atcaaaccgc cgccgctcga
     1741 tgatccggca ctggtcaggg aaggcgccga acactacgcc cagatgtgca cgggctgcca
     1801 tctggccccc ggcatcaccg agagtgaact tcgcgacgga ctgtatccgc ggccgccgaa
     1861 tctcacgcgc ttcgcccccg atccggcgga gtccttctgg atcatcaagc acggcctgaa
     1921 aatgaccgcc atgccggcgt ggggcaagac gcacgacgac cagaagatct gggcgatggt
     1981 cgcgttcctc cagaagcagc cgaagatgag tgccaccgag tatcgcggac tgaccgccaa
     2041 cgccggagca atgcacgaca tgcagccggc gccggcaccc acgggaacgg cgatgccgcc
     2101 gccggcgatg tccgccgcgc cggccccggc cggcgcggga acctcacccg caccggacca
     2161 cgggtagagc ggaacccgca agcggtttcc gccggaagcg tgcggcggaa gcggaacggc
     2221 ggaagtcgct gtgcggattc caccccacgc ggctgccgtg caggcgcgct tggcagcata
     2281 gtcgaacgcg gacgaagacc cttgactctg gactatggtc aagggtttag gatggctgca
     2341 tggacaagcc cgcgaaacgg ccggcggccc ggccagcccc cactgccccc ctcacgatcg
     2401 gcgcggtcgc gcgccgtgca ggcgtcggc
//
LOCUS       DASSNH010000286         7661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1345054, whole genome shotgun sequence.
ACCESSION   DASSNH010000286 DASSNH010000000
VERSION     DASSNH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345054"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFK00_10500"
     CDS             complement(<1..903)
                     /locus_tag="VFK00_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7593418.1"
                     /translation="MFAIRAAAGFGLGLVSALALAQTPEPHPRAATGYALIDTQHEVP
                     AMPPPTARHDVTETYFGTVVHDPYRWLEDADAPAVTQWIAAQNEHTEKLMEGFADAKA
                     IARRVGELALTSTQRSDPEIVGGTLFYLRQTPPQPQAVLVAQGWPQGGARVLVDPNAH
                     GGMAITGYWPSPDGQYVAYGTAEGGDEATTIRFVEVASGKALPDALPQAGGGTTPQAL
                     VWDADGKGVTYVRLPLPGTVPDDQLQFDAALYHHALGSDAKDDAVEFGKGLSKVAEYT
                     LLGSVGARHAAALLHYGDGAPDAVY"
     gene            1040..3526
                     /locus_tag="VFK00_10505"
     CDS             1040..3526
                     /locus_tag="VFK00_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7593419.1"
                     /translation="MNRTAVAGLACLLVVSAGAFAAAPQDAAPAMQASSHELTLEQIM
                     ADPDWIGPPVEDPYWSIDGRSIYFELKRKGSPLHDLYRVDANGGAPVKLGDAELATSD
                     GPAIFDRAHDRAAFLRHGDVFVRDVASSATRQVTRSDAGKSGLQFSADGRLLSWRQGD
                     DWYVWDADSGLVSQAAALEAKDDPADAKPDQLERLQLHLFSTLREDKADKQAMRERED
                     QLAAENPARAPAPFYLGDQVKVVTTSLSPDARWMVVVTEPKSYQRGKHPVVVHYVNES
                     GYPETEKARSYVGRNSPAPHALVLLDLRAHKQYKLSLDALPGIHDDPLHDLRTRRIAE
                     LKKQGHGDEAAALAAPKTRPVSVNFIPTADGYGPAIAWTDDASNVAIELVATDHKDRW
                     IETIDFNDHKLVTQDRLTDPAWINWSFNELGWLPDNRTLWFESEASGWAQLYVKPLDG
                     KPRALTGTHYEVSSPVVSPDGKWFYVLSNKQAPYAYDIYRLPTTGGDLERVTHFEGVS
                     CSECSRLFELSPDGSKLAVLHSSAYIPAQLAVANADGSGARELTDTRTAEYKALSSTW
                     VQPQFVEVPSSHGGFGIWSKYYQPVDYNTSHDHPAVIFVHGAGYLQDVSKGWSYYFRE
                     QMFNNLLTQEGYVVLDMDYRGSAGYGRDWRDAIYRQMGHPELEDLLDGKAWMVKNHHV
                     DPGRIGIYGGSYGGFMTEMALLRAPGEFAAGAALRPPSDWTSYNDGYTSDILNTPQLD
                     PEAYRISSPIEYAKNLQDPLLISHGLIDNNVMPADSIRLYQRFIELHKKNFWITLYPT
                     ERHAFEHADAWYDEYRRIHDLFSRFVKDRQ"
     gene            complement(3560..4369)
                     /locus_tag="VFK00_10510"
     CDS             complement(3560..4369)
                     /locus_tag="VFK00_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7593420.1"
                     /translation="MSKPAATMKYLRIGQITDRTGVSRKALRLYEARGLLAPDGRSAA
                     GYRLYGAKALTRLAEIGVLKRAGFTLSEIGKLLEHDGSAAALVEARIAALRVEVAAKS
                     RALVALERAWRGLASASQTIDQLVEDIQMNEELDMRFSKAEMAEFKHRAEALGKHFTP
                     DERERMRRRAEELGESRVQQVNAAWPGLIADVRTAMDAGTPPTDPAVQDMGRRWHALV
                     QAFTGGDAAIGRRMKEAYDKEPQVMAAQGMDHAMFAYIRDAMRAAGLELST"
     gene            4511..6565
                     /locus_tag="VFK00_10515"
     CDS             4511..6565
                     /locus_tag="VFK00_10515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7593421.1"
                     /translation="MRRPSTHIALLAAGLAFVLAPALAAAPRDTRSASIERILAQRAQ
                     VKEVPAVALSYDGQHLAWISSKGDQTILMLGSWTGRNAHAIAIPGVCREEGIRWAPRW
                     NELAVLTRCKVDPSNTKPIRGAIWVVDVHAGAAPRKVADIDGFAQDMQWSRDGKQIAF
                     LYVPGATRLPYATASGNPRVGVIGDTDVQVQGIAQVPAAGGTPAMLTPDGLYVYEFRL
                     SPIGSRMAYTAAPPPGDNNWWTAKLYVQDARAGAAPTIIADPATANGSLDGLQIALPR
                     WSPDNARVFFIGGLMSDRGATGGDIYAVAASGGEPVDLTPGVKVTPAWYTFLDARSLL
                     VTQIASGKVEVTEYTLDGNRARQTRTWFTAPGVIGDGRAALSVSLSTDSPRRIAYAAS
                     SFDQPPEVHAGVLGTRPPPAVTALNAGLEHTWGKSRSVEWNNDGRHVQGWLMYPADFD
                     PHKTYPMVVHVHGGPSSAMLPAWGAQATALSQFGYFVLMPNPRGSFGEGEAYAQAIRN
                     QMGYGDLDDILAGIDKIEKLVPVDDQRLGMIGWSYGGFMSMFAPTRTDRFKAVVAGAG
                     ISNWQSYYGQNQIDQWMIPFFGASLYDDPAAYAKSSAINFIKRTHTPALIVVGERDEE
                     CPAPQSFEYWHALKTLGVPTTLVVYANQGHHIADPKDLEDILRRTLDWFGKYLSGQGT
                     QD"
     gene            complement(6698..>7661)
                     /locus_tag="VFK00_10520"
     CDS             complement(6698..>7661)
                     /locus_tag="VFK00_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET7593422.1"
                     /translation="SAPRPRTEVNEERARSIISRNDSPDVAFEQSINPYRGCEHGCIY
                     CFARPSHSYLNLSPGLDFETRLFAKTNAAEQLARELAKPGYRCTPINLGANTDPYQPI
                     ERRYRLARAILEVLAATRHPCTIVTKNALVERDLDLLVPMAEKRLVQVFISCTQLDNH
                     LAAKLEPRASAPHRRIQAVRTLNEAGVPCGVLVAPIIPALTDRDMEHVLETASQAGAR
                     MAGYTILRLPWELKTLFREWLELNVPQRAEHVMSLVRQMRGGRENDPSFGTRMRGQGP
                     FAELLRRRFDVACRRHGFHRARELRLDCSRFVPPREPSPQGQLF"
BASE COUNT         1268 a         2655 c         2506 g         1232 t
ORIGIN      
        1 gtacaccgcg tcgggcgcgc cgtcaccgta gtggagcagc gcggcggcat ggcgggcgcc
       61 gaccgagccg agcagggtgt actcggcgac cttcgacaag ccttttccga attccaccgc
      121 gtcatccttc gcgtcgctgc cgagcgcgtg gtggtacagc gccgcgtcga actgcaactg
      181 gtcgtccggc accgtcccgg gcaacggcag gcgcacgtag gtcacgccct tgccgtcggc
      241 gtcccacacc agcgcctgcg gcgtggtgcc gccacccgct tgcggcagcg catccggcaa
      301 cgccttaccg ctggcgacct cgacgaaacg gatcgtggtg gcctcgtcgc cgccttcggc
      361 ggtgccgtag gcgacgtact ggccgtccgg cgacggccag taaccggtga tcgccatgcc
      421 gccgtgggcg ttggggtcga ccagcacccg cgcgccgcct tgcggccacc cctgcgcgac
      481 cagcacggcc tgcggctgcg gcggggtctg gcgcaggtag aacaaggtgc cgcccacgat
      541 ttccggatcg gaacgctggg tggaggtgag cgccagttcg ccgacccgcc tggcgatcgc
      601 cttggcgtcg gcgaagcctt ccatcagctt ctcggtatgc tcgttctgtg ccgcgatcca
      661 ttgcgtgacg gccggtgcgt cggcgtcttc cagccagcgg tacgggtcgt gcaccacggt
      721 tccgaagtac gtttcggtga cgtcgtgcct ggctgtcggt ggcggcatgg cgggaacctc
      781 gtgctgggtg tcgatgagcg cgtagccggt ggccgcgcgc gggtgcggct cgggcgtttg
      841 cgccagcgcg agggcggaca cgaggccgag gccgaagccg gcggcggcac ggatcgcgaa
      901 catgctcgga ctccggaaac gatgggttcc gcgcagggta gcgcacgccg gccgctgcgc
      961 acacgacccc ggacttccgg cgcttgcgcc tgcatgcggc gtttggctag gctccggtct
     1021 tttgctcggg gccacctcca tgaatcgcac tgcagtcgcg ggtcttgcct gcctactggt
     1081 cgtcagcgcg ggcgcgttcg cggccgcgcc acaggatgcg gcgccggcca tgcaagccag
     1141 cagccacgaa ctcaccctcg agcagatcat ggccgatccc gactggatcg ggccgccggt
     1201 cgaggacccc tactggagca tcgacggccg cagcatctac ttcgagctga agcgcaaggg
     1261 cagccccctg cacgacctgt atcgagtcga tgcgaacggc ggcgcgccgg tgaaactcgg
     1321 cgacgcggaa ctggccacgt ccgacggtcc ggcgatattc gaccgcgcgc acgaccgcgc
     1381 ggcgttcctg cgccacggcg acgtgttcgt gcgcgatgtc gcgagcagcg cgacccgcca
     1441 ggttacccgc agcgacgccg gcaagtccgg cctccagttt tccgccgacg gccgactgct
     1501 gagctggcgg cagggcgacg actggtatgt gtgggacgca gactccggcc tggtgtcgca
     1561 ggccgccgca ctggaagcca aggacgaccc ggccgatgcc aagccggacc agctcgagcg
     1621 cctgcagctc catctcttct cgacgttgcg cgaagacaag gccgacaagc aggccatgcg
     1681 cgaacgcgaa gaccagctcg ccgccgaaaa cccggcacgc gcgcccgcac cgttctacct
     1741 cggcgaccag gtcaaggtcg tgaccacgtc gctgtccccg gacgcgcgct ggatggtggt
     1801 ggtgaccgag ccgaagtcgt accagcgcgg caagcatccg gtagtcgtgc actacgtcaa
     1861 cgagtccggc tatcccgaaa ccgagaaagc ccgcagctac gtaggccgca attcgccggc
     1921 gccgcacgcg ctggtcctgc tggacctgcg cgcccacaag caatacaagt tgtccctcga
     1981 tgcgctgccc ggcatccacg acgatccgct gcacgacctg cggacccggc gcatcgccga
     2041 gctgaagaag cagggccacg gcgacgaggc cgctgcattg gccgcaccga agacgcgtcc
     2101 ggtatcggtg aacttcattc cgaccgccga cggctatgga ccggcgatcg cctggaccga
     2161 cgacgccagc aacgtcgcga tcgaactggt cgccaccgac cacaaggatc gctggatcga
     2221 aaccatcgac ttcaatgacc acaagctggt cacgcaggac cgcctcaccg atccggcctg
     2281 gatcaactgg agcttcaacg aactcgggtg gctgccggac aaccggacgc tgtggttcga
     2341 gtccgaagcc agcggctggg cgcagttgta cgtgaagccc ctggacggaa agccgcgcgc
     2401 actgaccgga acccattacg aagtgtccag cccggtggta tcgccggacg gcaagtggtt
     2461 ctatgtgctc tccaacaagc aggcgcccta cgcctacgac atctatcgcc tgccgaccac
     2521 gggcggtgat ctcgagcgcg tcacgcattt cgaaggcgtg tcctgctccg aatgcagccg
     2581 cctgttcgag ctgtcccccg acggcagcaa gctcgcggtg ctgcattcca gtgcgtacat
     2641 cccggcgcaa ctcgcggtgg cgaacgcgga cggctcgggc gcgcgcgagc tcaccgacac
     2701 ccgcacggcc gaatacaagg cactctcgtc cacctgggtg cagccgcaat tcgtcgaggt
     2761 ccctagcagc catggcggct tcgggatctg gtccaagtac taccaacccg tcgattacaa
     2821 cacctcgcac gaccatcccg cggtgatctt cgtgcacggc gcaggctacc tgcaggatgt
     2881 cagcaagggc tggtcgtact acttccgcga gcagatgttc aacaacctgc tgacccagga
     2941 aggctacgtg gtgctcgaca tggactaccg tggatcggcc ggctacgggc gcgactggcg
     3001 cgacgcgatc taccggcaga tgggccatcc ggagctcgag gacctgctcg atggcaaggc
     3061 ctggatggtg aagaaccacc acgtcgatcc cggccgcatc ggcatctacg gaggttccta
     3121 cggcggcttc atgacggaga tggcgctgct gcgcgcgccg ggcgaattcg ccgcaggcgc
     3181 cgcgctgcgt ccgccatcgg actggaccag ctacaacgac gggtacacct ccgacatcct
     3241 caataccccg cagctcgatc ccgaggcata ccggatttcc tcgccgatcg aatacgcgaa
     3301 gaacctccag gacccgctgc tgatcagcca cggcctgatc gacaacaatg tgatgccggc
     3361 cgactcgatc cgcctgtacc agcgcttcat cgagctgcac aagaagaact tctggatcac
     3421 cttgtacccg accgagcgcc acgcgttcga acatgcggac gcctggtacg acgaataccg
     3481 ccgcatccac gacctgttca gccggttcgt gaaagacagg cagtgaaaga ggggggcgtc
     3541 tcgcgacgcc ccgcttgctt cacgtggaca gctccaaccc cgccgcccgc atcgcatcgc
     3601 ggatgtaggc gaacatcgcg tggtccatgc cctgggccgc catcacctgc ggctccttgt
     3661 cataggcttc cttcatccgg cgcccgatcg cggcgtcgcc gcccgtgaat gcctggacca
     3721 aggcgtgcca gcggcggccc atgtcctgga ctgcgggatc ggtcggcggc gtgccggcat
     3781 ccatcgccgt gcgcacgtcg gcaatcagcc cgggccacgc ggcgttcacc tgttgcacgc
     3841 gtgattcgcc gagttcctcg gcgcgccggc gcatccgttc gcgctcgtcg ggcgtgaagt
     3901 gcttgcccag cgcttcggcg cgatgcttga actcggccat ttcggccttg ctgaatcgca
     3961 tgtccagttc ttcgttcatc tggatgtcct ccacgagttg atcgatggtt tgcgatgccg
     4021 atgccagtcc ccgccaggcg cgttccagcg cgacgagcgc acgcgacttg gcagccacct
     4081 ctacccgcag cgccgcgatg cgcgcttcca ccagcgccgc ggccgagccg tcgtgttcga
     4141 gcagcttgcc gatctcggaa agcgtgaagc cggctcgctt cagcacccct atttcggcga
     4201 ggcgcgtcaa cgcctttgcg ccgtacaggc gatagcccgc cgcggaccgg ccatcgggtg
     4261 ccagcaatcc gcgcgcctcg tacaggcgca gtgccttgcg gctgacaccc gtgcggtcgg
     4321 tgatctgccc gatcctcagg tacttcatgg tggcggctgg cttcgacatc gcgcgcacac
     4381 actacggcct gcccttggga caaggtcaat agccatctgc ggcgcaacgg cgcgcggcac
     4441 gcctttcgtc acttggcgca cgaccagacc cggtgtacgg tgcgtcgctt caccttcgcg
     4501 gacgccttcc atgcgtagac cttccaccca tatcgcactg ctggctgccg gcctcgcgtt
     4561 tgtcttggcg ccggcgctcg cggccgcgcc gagagacacc cggagtgcca gcatcgaacg
     4621 gatcctcgca caacgtgcgc aggtgaagga ggtgccggcg gtcgccttgt cctacgacgg
     4681 ccagcacctc gcgtggatca gctccaaggg cgaccagacc atcttgatgc tcggctcctg
     4741 gacgggccgc aacgcccacg ccatcgcgat ccccggagtc tgccgggaag aaggcatccg
     4801 ctgggcgccg cgctggaatg aactggcggt gctgacgcgt tgcaaggtcg atccctccaa
     4861 caccaaaccc atccgcggcg ccatctgggt ggtcgacgtg cacgccggcg ccgcgccgcg
     4921 caaggtcgct gacatcgatg gcttcgcgca agacatgcag tggtcgcgcg acggcaagca
     4981 gatcgcattc ctgtacgtgc ccggcgcgac ccggctgccg tatgcgaccg cctcgggcaa
     5041 cccgcgggtc ggcgtgatcg gcgacaccga cgtgcaggtg caggggatcg cgcaggtccc
     5101 ggccgcgggc ggaacgcccg cgatgctgac gcccgatggt ttgtacgtct acgagttccg
     5161 gctctcgccg attggcagcc gcatggctta cacggccgcg cccccgcccg gcgacaacaa
     5221 ctggtggacc gccaagctgt acgtgcagga cgcgcgcgcc ggcgcagcgc cgaccatcat
     5281 cgccgatccg gccaccgcaa acggatcgct cgacggactc cagatcgcgt tgccgcggtg
     5341 gtcgcccgac aacgcccgcg tcttcttcat cggcgggctg atgagcgacc gcggcgcgac
     5401 cggcggcgac atctacgccg tggccgcgtc cggtggcgaa cccgtcgacc tgaccccggg
     5461 cgtgaaggtc acgcccgcgt ggtacacgtt cctcgatgca cgttcgctgc tggtcaccca
     5521 gatcgcgagc ggcaaggtcg aggtcaccga atacacgctc gatggcaacc gggcgcggca
     5581 gacgcgcacc tggttcaccg caccgggcgt gatcggcgat gggcgcgccg cgctctcggt
     5641 gtcgctgtcg acggactcgc cgcgtcgcat cgcctacgct gccagttcct tcgatcaacc
     5701 gcccgaggtc cacgcgggcg tgctcggcac ccgtccgccg cctgccgtga ccgcgctcaa
     5761 cgccggcctc gagcacacct ggggcaagtc gcgttcggtg gaatggaaca acgacggccg
     5821 ccatgtgcag ggttggctga tgtatccggc cgacttcgac ccgcacaaga cctacccgat
     5881 ggtcgtgcat gtccacggcg gtccatcgtc ggcgatgctg ccggcctggg gcgcacaggc
     5941 gacggcgctg tcgcagttcg gctacttcgt gctgatgccc aacccgcgcg gcagcttcgg
     6001 cgagggcgag gcctacgcgc aggcgatccg caaccagatg ggctacggcg acctcgacga
     6061 catcctcgcg ggtatcgaca agatcgagaa gctcgtgccg gtcgacgatc agcgcctcgg
     6121 gatgatcggc tggagctacg gcggcttcat gagcatgttc gcgcccaccc gcaccgatcg
     6181 cttcaaggcc gtggtggcgg gcgcggggat ttccaactgg cagagctatt acggccagaa
     6241 ccagatcgac cagtggatga tcccgttctt cggtgcctcg ctgtacgacg atcccgcggc
     6301 gtacgcgaag agttccgcaa tcaacttcat caagcggacc cacacccccg cgctgatcgt
     6361 ggtgggcgag cgcgacgagg agtgccccgc gccgcaatcg ttcgaatact ggcacgcgct
     6421 gaagacgctc ggcgtgccga cgacgctggt cgtgtacgcc aaccagggcc accacatcgc
     6481 cgaccccaag gacctggagg acatcctgcg ccgtacgctg gactggttcg ggaaatatct
     6541 ttccggacag ggcacgcaag actgacgggc tacagcaggc ggcggcagga agcttatcga
     6601 cgcggatttg cgcggatgaa cttggctcac ctgcgatctg cttgatccgc gttgatccgc
     6661 gtcgaatgcc tttttcccgt gagtgccaat cgccccgcta gaacaactgc ccctgcggcg
     6721 acggctcgcg cggcggcacg aagcgcgagc aatccagccg aagttcacgg gcgcgatgga
     6781 agccgtggcg ccggcaggcc acgtcgaagc ggcgccgcag caattccgcg aacggcccct
     6841 ggccgcgcat gcgggtgccg aatgacggat cgttctcgcg gccgccgcgc atctggcgga
     6901 ccaggctcat cacgtgttcg gcacgctgcg gtacgttgag ttccagccac tcccggaaca
     6961 gcgtcttcaa ttcccagggc aagcgcaaga tggtgtagcc cgccatccgc gcgcccgcct
     7021 gcgaggccgt ttccagcacg tgttccatgt cgcggtcggt caacgccggg atgatcggcg
     7081 cgaccagcac gccgcagggc acgccggctt cgttcaaggt cctgaccgcc tggatgcgcc
     7141 ggtgcggcgc cgacgcacgc ggctccagct tcgcggcgag gtggttgtcg agctgggtgc
     7201 aggagatgaa tacctgcacc aggcgctttt ccgccatcgg caccagcagg tccaggtcgc
     7261 gttcgaccag cgcgttcttg gtcacgatgg tgcagggatg gcgggtcgcc gcgagcactt
     7321 ccaggatcgc acgggccagg cgataacgcc gttcgatcgg ctggtacgga tcggtgttgg
     7381 cgcccagatt gatcggcgtg cagcggtaac ccggcttggc gagctcgcgc gccaactgtt
     7441 ccgccgcatt cgtcttggcg aacagccggg tctcgaagtc cagtcccggc gagagattga
     7501 gatagctgtg cgacggccgg gcgaagcagt agatgcagcc gtgctcgcag ccgcggtagg
     7561 gattgatcga ctgctcgaat gccacgtcgg gcgaatcgtt gcggctgatg atcgagcgcg
     7621 cgcgctcttc gttcacttca gtgcgcggcc gcggcgcgga c
//
LOCUS       DASSNH010000287        18274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1426387, whole genome shotgun sequence.
ACCESSION   DASSNH010000287 DASSNH010000000
VERSION     DASSNH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426387"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..686
                     /locus_tag="VFK00_10525"
     CDS             147..686
                     /locus_tag="VFK00_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7593423.1"
                     /translation="MANLRAYRGVEPRLGARVYVDPAATVIGDVELGDDVSLWPGAVV
                     RGDVEKIRIGARSNVQDTAVLHVTHDGPYSPGGFPCIVGDDVTIGHGAVVHACTIGDC
                     CLIGMHATLLDGVVVKRHAFVGAGALVAPGKVVGEGELWVGSPARCVRRLSDSEVEAL
                     RYSANHYVELKDEYLAMVL"
     gene            complement(745..1575)
                     /gene="aroE"
                     /locus_tag="VFK00_10530"
     CDS             complement(745..1575)
                     /gene="aroE"
                     /locus_tag="VFK00_10530"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7593424.1"
                     /translation="MPLPRYAVFGHPTAHSLSPRLHALFAAQTGMGLEYVAIDAPPAD
                     FPAAIRSFFKKGGSGANVTLPHKAAAFDVADVPTATAERIRVANVLTRLPDGRIEADS
                     NDGAGMLTDLRARAGIDPHGCDVLLLGAGGAARATAFALLDAKVRTLVIANRTFTRAQ
                     ALAAALVQPERVRVRRWEALDSRTPFHLIVNATSAGVRREPLALPGTLADTESVGYDL
                     SYGEAAQPFLEWANAAGCARACDGLGMLVETAAASFHCWHGVQPDTDTALDTLRRTLL
                     "
     gene            1790..2128
                     /locus_tag="VFK00_10535"
     CDS             1790..2128
                     /locus_tag="VFK00_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593425.1"
                     /translation="MHRKTATVTPTRRSAGTHRSHRQPFRHGISFAPACDRFTGGRPL
                     RRPQPGATPDPITLGASTWLACGIVLLGLTPLPLRDTHLGWSLTFWALVAPSVILLAR
                     RLRPRPPRSR"
     gene            complement(2120..3628)
                     /locus_tag="VFK00_10540"
     CDS             complement(2120..3628)
                     /locus_tag="VFK00_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HET7593426.1"
                     /translation="MSRLAIVVEKASDWGSYYPSDNVVTAMQYLREPVGGDERTHVIN
                     LCRSYKYLGIGHYVSLLGEARGHRVIPSVRTISDLRKRALYNLDVEDLNQKLAHFLPA
                     GGQDTTDLGILVYFGVTSYPALQDLARQVFETFPCPLLRIEFERDRSWQISAIKPVGL
                     HTLDDAQEDAFAQALDAFSKRLWRKPRTRKQYRYDLSMLVDPKETMPPSNARALKHFI
                     AAGRELGIDVDTVTKQDYPRLAEYDGLFIRETTAPDNHTYRFAHKAEREGMVVIDDPT
                     SILRCTNKIYLNDLLRSKKLAVPRSEVLYREDAKQLRELPARLGFPLVLKIPDGSFSR
                     GVVKVEDEKHLVEAAQHLFQSTALLIAQEFLYTEFDWRIGVLNRKPLYACKYFMSRGH
                     WQIYNHSARGSARSGGFETLRLEDVPPDVVRLAVRGTAPIGTGLYGVDIKQTRDRAVL
                     IEVNDNPSIDAGVEDAELGEDLYRSIMAELLRRMEAKRIGISTQASARGASP"
     gene            3728..4201
                     /locus_tag="VFK00_10545"
     CDS             3728..4201
                     /locus_tag="VFK00_10545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7593427.1"
                     /translation="MRMTPDPAARIREASPDDLDALVALERVTFESDRISRPQWRRHI
                     GSDSACVLVAGPRDRIDGAAVVFHRRNSRRARLYSLAVAAASRGRGLAKALLAAAEAD
                     ARRHGCNVLDLEVRTDNRAAIALYEHHGYRRTTRLQGFYEDGADGWRFTKALADP"
     gene            4377..5264
                     /locus_tag="VFK00_10550"
     CDS             4377..5264
                     /locus_tag="VFK00_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HET7593428.1"
                     /translation="MKRSLTLGLLSAAILAITAPAATAASPMATTTGSPAPASSSSAP
                     AVPAKPVVERPKADATIDRIITAMNNSSTWGHPDLFGEFAGMRLFYEGQFKAAMKYFE
                     YGARYADKFSQLSIGLMYENGRGVAKDPVTACAWIALAAERKYPTYIATRDRVCEALT
                     PAQHDQAVAVLDKLLPEYGDAVAKPRMVRALERARTEMTGSRTGFDFGAESVGVMKGG
                     CGGPTIEGTSQAGCGSTDFWSPWRWDPKKYFQMRDAHWRGVVTVGSLEKESQTHVKAA
                     GAGKNPAQAAPAGASSSGH"
     gene            5299..5550
                     /locus_tag="VFK00_10555"
     CDS             5299..5550
                     /locus_tag="VFK00_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HET7593429.1"
                     /translation="MFLIGIPNMRRHVVSASLLLFGAALLAGCGQKGPLFLPKPVAPA
                     APASPAAPASSVAAPAASVAPAPPQPVAGATSAAPARSG"
     gene            5562..6395
                     /gene="dapF"
                     /locus_tag="VFK00_10560"
     CDS             5562..6395
                     /gene="dapF"
                     /locus_tag="VFK00_10560"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7593430.1"
                     /translation="MGLRFTKMHGLGNDFVVLDARTDALQIEASVIQAMSDRHKGIGF
                     DQLLVIERPQDRTCVAAYSICNADGSRAEQCGNGARCIGAWLHRDGVLAPGTEARLES
                     PAGVVAMRLADAATVAVDMGEPVFEPARIPFDAPAFAATYPLEIDGDHLEIAAVSMGN
                     PHAVMEVADVADPRVDRLGPRITTHPRFPEGANAGFVQIVDPQTIRLRVHERGAGWTL
                     ACGSGACAAVAALHRRGRVGNRVFVELPGGTLEIAWRGIGHPLWMTGPAAFVFDGEWL
                     G"
     gene            6502..7185
                     /locus_tag="VFK00_10565"
     CDS             6502..7185
                     /locus_tag="VFK00_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HET7593431.1"
                     /translation="MSELTLKQSLEAMDVAEYLRQHPRFLEHFPDVAAELVMPREHGP
                     AASLAAYQLETLRARNRELNQRLAELVEVAGDNEQLMVRVHTLTVGLLRDDNLADTCK
                     SFVAALHEDFHTDLVRLVLFRAGADLPQAGWMVAVPEGPRAWPEFAEFLSRGEPACGR
                     LAPEKLERLFGGETARVQSAALLRIGGLGMLAIASDDPNRFHPGIGTIFLKLIAQALD
                     AAISRFPAE"
     gene            7187..8092
                     /locus_tag="VFK00_10570"
     CDS             7187..8092
                     /locus_tag="VFK00_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET7593432.1"
                     /translation="MNGALAADAAGFLEYLKVERRYSPGTLRNYTHALDALTRFADGL
                     AIGNWSELRGEQLQTFIASEHRRGLEPPSLRAMLSAFRSFFRFLARDGRIQNNPAAGV
                     RSPKVRRKLPEVLDVDEAATLVEVAAGDPLALRDHAMLELLYSSGLRVSELCGVRWRD
                     LDAEQGLLRVTGKGNKTRIVPVGRMALSALTALREAQPADADEPVLRGRGGRALTAGA
                     VRAALKRRARQQGVWKRVYPHLLRHSCASHLLESSGNLRAVQELLGHADIGTTQIYTH
                     LDFQHLAKVYDAAHPRAKRKRGTGG"
     gene            complement(8217..10079)
                     /locus_tag="VFK00_10575"
     CDS             complement(8217..10079)
                     /locus_tag="VFK00_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7593433.1"
                     /translation="MIASATPIVESMATAWKAGDTQRVLVLAAQASDVEADDEAFLAL
                     LGMARQQAGDYSRAAQTFGRLTSIQPDVAAWWNNLGESSRQAGDLAVAERALSKARAL
                     APGDADVLYNLGLLYIQQQRWALARGVLLDAARLAPRFIEARLEAAHACHVCGDLEGE
                     EAMLEGAGAWPPQPASQALVLASILSILGRLEIALDVLAQARLPEGPAADAMRLRITA
                     QRVALYERNNRVDQARCELQQLPLDALDALGAGGIDARIDVWRAHAVMAMREGRPAVA
                     AALFQRVLDVAADPEVVSNAAFGLASACDRQAQYREAWSALEVAHAAQMEIVRQAAPE
                     LSEASGQPLPMVSRSVDARAYATWKPVKGPSTARSPVFVIGFPRSGTTLLEQMLDAHT
                     DFRSMDERGYIYQLIQRMEHAEQKYPADLAALTQLEADQLRSVYGRLVGRTLPDLGQR
                     RLVDKNPLNMLCLPMILRLFPHACIVLCIRHPCDVLLSCSMQAFRSPAFRSMCSALPR
                     LARGYTEAFEQCCGHIEVFKPRVLEWRYESVVADPAAAVSRLGDFLAVADASAMMDFA
                     GNARRKQFIATPSYAQVTQPLNKAAVGRWMHYREMFEPVLPVLRPWIDRLGYDL"
     gene            complement(10185..13052)
                     /locus_tag="VFK00_10580"
     CDS             complement(10185..13052)
                     /locus_tag="VFK00_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7593434.1"
                     /translation="MNHELNRLTLAVRLGLTAGLLAATGMAQAQQPPNGQSQTANPAD
                     QDQANSQPKTLQTVVVTGSLIRRVDTETASPVTIIDRQNITSSGKPTLGDILQQLPSV
                     AGNATNPQNNSNGGGVASPLTEGGDGASRVSLRGLGTGRTLVLIDGQRMLNADMNLVP
                     QNMVQRIDVLAEGASTTYGSDAVGGVVNILLRHDFDGVEVSANGGISGHGDGQRHGLS
                     LTMGHTGDRYNIVGGINYNKYDAVPNTRRDFAAHQLYLTDGQISVAGSSSTPNGRMQL
                     PGSFAAQYGCTPDSTDHIVVTRDAGDGTTLGDYRCRTSSDTFNYAAFNYIQVQQQRTD
                     GFLVGSYNLTDNITAFVDAFYNHTESAGQDASIPVGTGDGLVIPATAPFNPFGVTFSP
                     NPIPGDPNSGYVYQTRLTGVGTRIHPYTTSTGQLIAGLRGALGQSSWNWNVTVDYGHT
                     SRTQNDENELYIPALQAAIDAGANIFDQANNGAALGAGAVDAIYRKREILRQISAGAN
                     GNLFSLPAGDVQLAVGALYRKQSMNYTIDPLALLDPVTGFCQISQEACGSPGSGNDSV
                     KEAFAETLIPLLKEVPGAYSLNVDVGVRYSDYNSAGSTTNGKVAIEWRPIPDLLVRGT
                     VSQVFRAPNLDQLYDGRSLVQPALNDPCAHLSAAELAQHPVACQYVPPDWAGNSPAQV
                     NTYYSGANVVGGNLKPEHGKSVDFGLVYNPSWAQGLNTSLDFWHIYLYDTLVAIDGRT
                     VVDACFNNGASPFCPFIHRYDNTTQQPGQVFYIDTPVVNLGSLSTSGIDFTANYKLPH
                     FDFGGFDPGDFKLGLATSYVSTFNNNKTPGLPGSTVDKLAGTWNAQFGNVSRWRATAT
                     LGWDRGNWDAQWRTRYISGVTALNADYATGASLPVASVVYNDVQVGVAVPGIHTRFDI
                     GVDNLFDKRPPLIYHNGQGNTDSATYDMMGTYYWARATLKF"
     gene            13410..13976
                     /gene="hslV"
                     /locus_tag="VFK00_10585"
     CDS             13410..13976
                     /gene="hslV"
                     /locus_tag="VFK00_10585"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7593435.1"
                     /translation="MESFHATTILSVRKNGKAVLGGDGQVTLGNTVVKANARKIRRLG
                     KNGDVLAGFAGATADAFTLFELFEDKLAKHSGQLTRAAVEMAKEWRTNRRLGRLEAML
                     AVVDKDASLLISGNGDVLEPEHGLIAIGSGGPYALAAARALMENGDLDARQIVEKALG
                     IAGDICIYTNHNVAIEELDSGNREQGTG"
     gene            14041..15396
                     /gene="hslU"
                     /locus_tag="VFK00_10590"
     CDS             14041..15396
                     /gene="hslU"
                     /locus_tag="VFK00_10590"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7593436.1"
                     /translation="MSELTPREIVNELDRFIIGQHNAKRAVAIALRDRWRRMQLDPAF
                     RNEVTPKNILMIGPTGVGKTEIARRLATLAKAPFVKIEATKFTEVGYVGKDVDSIVRD
                     LADVAYKLTRDQAKERVRSQAEEHADERILDALLPRREAGGGWDQPADAETARIESDA
                     STRTKLRKQLRDGALDEREIELEFSMNVGVQIMAPPGMEEMSQQLSSMFQHLGGGKTQ
                     KRKLKIKAARPLLIDEEAGKLLNDDELRAQAIENCEQNGIVFIDEIDKVAQRSDWGTT
                     GAGVSREGVQRDLLPLVEGSTVSTKYGPVKTDHVLFIASGAFSMAKPSDLIPELQGRL
                     PIRVELSALSVDDFKRILEEPNNALTKQYAAMLKTEGVTLEFKDEGIARLAEVAFDVN
                     ESTENIGARRLQTVMERLLETISFEAADKSGETYVIDAEYVDKNLKDLASDEDLSRYI
                     L"
     gene            15455..16069
                     /locus_tag="VFK00_10595"
     CDS             15455..16069
                     /locus_tag="VFK00_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593437.1"
                     /translation="MGKVLKFESESPRPKLRGALQRGELVRVWRSGLEDGSFCGYLAG
                     IGREWLLMWVVGDQIGFDGMYAMRLADITEVEAPDSHHVFMEKALAVRNIHPRLPHNF
                     PLDDITQVVRAAAEYAAVMCVHVDTEDEHEVCYIGRPLGIEDDGFTLQEISPDAEWLR
                     EPSFFGWDEISTVSFDEPYAQVLLDVAGQPPRIDFAGSGFGSAN"
     gene            16321..16731
                     /locus_tag="VFK00_10600"
     CDS             16321..16731
                     /locus_tag="VFK00_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET7593438.1"
                     /translation="MPAVPKPTDITLHKKSRTLEVAFDSGERFVMPCEYLRVHSPSAE
                     VQGHGPGERVLVPGKRDVGISAIEPVGNYAVLLRFSDGHATGIFSWETLYDLGINQET
                     NWQSYLDALEKNQLSREPKSPYGHPVPMRGPKPR"
     gene            complement(16823..17314)
                     /locus_tag="VFK00_10605"
     CDS             complement(16823..17314)
                     /locus_tag="VFK00_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7593439.1"
                     /translation="MSEESETFEGGCDCRFIRYRLTRTPLFVHCCHCRWCQRETGSAF
                     VLNALIETESVERLHGEPEMVMTPSASGSGQDIARCPVCRVALWSRYAGAGPLFTFIR
                     VGTLDDPDRFPPDIHIFTTSKQPWVILPDGVPAMPGYYDREQCWPKESLKRRAAVLAK
                     AST"
     gene            complement(17476..>18274)
                     /locus_tag="VFK00_10610"
     CDS             complement(17476..>18274)
                     /locus_tag="VFK00_10610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7593440.1"
                     /translation="LNHRIAPFILRRTKAQVVSELPEKTEIARRVVLSGKQRELYDSL
                     RVSLGDEVREVIRQRGIEHSGIIVLDALLKLRQTCCDPRLVKLESARGIRESAKLELL
                     MEMLPALLSEGRRVLLFSQFTEMLALIARELNRRHLQYVTLTGDTRDRAEPVRKFQEG
                     EVPLFLLSLKAGGVGLNLTAADTVIHYDPWWNPAAETQASDRAHRIGQDKPVFVYRLI
                     CADTVEERIEDLKARKAELAQAVLEGGGTRDKLAFDEEDLNALFATE"
BASE COUNT         3142 a         6081 c         5993 g         3058 t
ORIGIN      
        1 atgcgctgcg cgaacacact gctccgaccg gaaaatcgtg aaccgacgcg ggcgcggccg
       61 gtgcaggacg cacccgggcg cgggcgtacc gccggatcca tcccttcgtt acgatggcgc
      121 gtcgtgatcc gccaggaaca attgacatgg ccaacttgcg tgcttatcgg ggtgtcgagc
      181 cacggctcgg cgcgcgcgtg tatgtcgacc ccgccgccac cgtgatcggc gatgtggaac
      241 tcggcgacga cgtgtcgctt tggcccggcg cggtggtgcg tggtgacgtc gagaaaatcc
      301 gcatcggcgc gcgcagcaac gtgcaggaca ccgcggtgct gcacgtgacc catgacggcc
      361 cgtactcgcc cggcgggttc ccgtgcatcg tcggcgacga cgtcacgatc gggcatggtg
      421 ccgtggtgca tgcctgcacg atcggcgact gctgcctgat cgggatgcac gcgaccctgc
      481 tggacggcgt cgtggtcaag cggcacgcgt tcgtcggcgc gggcgcgctg gtcgcgcccg
      541 gcaaggtcgt cggcgaaggc gagctgtggg tgggcagccc cgcgcgctgc gtgcgtcggt
      601 tgagcgactc cgaggtcgag gcgctgcgtt actcggccaa ccattacgtc gaactcaagg
      661 acgaatatct ggcgatggtt ttgtagcagc gcgccatgcg cgcgacggaa ccgcgtcggc
      721 acgtgtcgcg cgcatggcgc gttcctacaa cagcgtgcga cgcaatgtgt ccaatgccgt
      781 atcggtgtcc ggctgcaccc catgccagca atggaacgag gcggcggccg tttcgaccag
      841 catcccgaga ccatcgcagg cacgtgcgca gccggcagca ttcgcccact cgaggaacgg
      901 ctgtgcggcc tcaccatagc tgaggtcgta cccgacggat tccgtatcgg cgagtgtccc
      961 cggcaacgcc agcggttcgc ggcgtacgcc cgcggaggtc gcattcacga tcaggtggaa
     1021 cggcgtccgc gaatccaggg cctcccagcg cctgacccgc acccgttccg gctgcacgag
     1081 tgcggccgca agcgcctgcg cacgcgtgaa agtgcggttg gcgatcacca acgtgcgaac
     1141 cttggcgtcc agcagcgcga acgcggtcgc ccgcgcggcg ccgccggcac cgagcaacaa
     1201 cacgtcgcat ccgtggggat cgatgccggc acgcgcacgc aggtcggtga gcatcccggc
     1261 gccatcgttg ctgtcggcct cgatccggcc atccggcagg cgtgtcagca cgttggccac
     1321 gcggatgcgt tcggctgttg ccgttgggac gtcggcaacg tcgaatgcgg ccgccttgtg
     1381 cggcagggtc acgttggccc cgcttccacc cttcttgaaa aacgagcgga tcgcggccgg
     1441 aaagtccgcg ggcggcgcat cgattgcaac gtattcgaga cccatgcccg tctgcgccgc
     1501 gaacagcgca tgcagccgcg gcgacagcga gtgggcagtg gggtgaccga agacggcgta
     1561 acgcggaagg ggcatgggct gcctcgaggc tcctgttctg ccccaccctt ccgtctcccc
     1621 ggaaggaaga tggggactcg acgaatcgcg ctgcgcgcga cggtttgtcg atggagcggg
     1681 gactccgcaa agcgtagcaa cacccgcaac gctcaggtgg cgattgcgtg tcgcagtccg
     1741 cgagaccaag gccgcacaga ctgcccgcaa ccatcaccgg agccgcccca tgcaccgcaa
     1801 aaccgcgacc gttaccccta cgcgccgtag cgccggcacc caccgcagtc atcggcaacc
     1861 gttccgccac ggcatctcgt tcgctcccgc gtgcgaccgg ttcaccggcg gccggcccct
     1921 gcgacgcccg cagcccggcg caacgccgga cccgataacg ctcggcgcaa gtacctggct
     1981 tgcctgcgga atcgtgctgc tgggactcac gcccttgccg ctgcgcgaca cccatctcgg
     2041 ttggtcgctc acgttctggg ccctggtcgc gccgtccgtc atcctgctgg cgcgccgcct
     2101 gcgcccgcgg ccgccgcggt cacggtgaag ccccgcgcgc cgaggcctgc gtggaaatcc
     2161 cgatgcgctt ggcttccatc cgccgcagca actcggccat gatgctgcgg taaagatcct
     2221 cgcccagttc ggcatcctcg acgccggcat cgatcgacgg gttgtcgttc acctcgatca
     2281 gcaccgcgcg atcgcgggtc tgcttgatgt cgacgccgta aaggccggtc ccgatcgggg
     2341 cggtgccgcg tacggcgagc cgtaccacgt cgggcggcac gtcctcgagc ctcagtgttt
     2401 cgaatccacc cgagcgggcg ctgccgcggg cgctgtggtt gtagatctgc cagtgcccgc
     2461 gcgacatgaa gtatttgcag gcgtacagcg gcttgcggtt cagcacgccg atccgccaat
     2521 cgaattccgt gtacaggaat tcctgcgcga tcaaaagggc agtgctctgg aacaggtgct
     2581 gggcagcctc gaccagatgc ttttcatcct cgaccttgac cacgccgcgc gaaaacgagc
     2641 cgtccgggat cttcaacacc agcgggaagc ccagccgtgc cggcagttcc cgcaactgtt
     2701 tcgcgtcttc ccggtacagg acttccgagc gcggcaccgc gagcttcttc gaacgcagca
     2761 ggtcgttgag gtagatcttg ttggtgcagc gcaggatcga ggtcggatcg tcgatcacca
     2821 ccatgccctc gcgctcggcc ttgtgcgcga agcggtacgt gtggttgtcg ggtgcggtgg
     2881 tttcgcggat gaacagcccg tcgtattcgg ccagccgcgg atagtcctgc ttggtgacgg
     2941 tgtcgacgtc gatgccgagt tcgcgaccgg cggcgatgaa gtgcttcagc gcgcgggcgt
     3001 tggaaggcgg catcgtctcc ttgggatcga ccagcatcga gaggtcgtag cggtactgct
     3061 tgcgcgtgcg cggcttgcgc cacaggcgct tggagaaggc atcgagggct tgcgcgaaag
     3121 cgtcttcctg ggcgtcgtcg agtgtatgca gtcctaccgg cttgattgcg ctgatctgcc
     3181 acgagcggtc ccgctcgaac tcgatccgca gcagcggaca gggaaacgtc tcgaacacct
     3241 gccgcgcaag gtcctgcagc gccgggtacg aggtaacgcc gaaatacacc aggatcccga
     3301 gatcggtggt gtcctgcccg ccggccggca ggaagtgcgc aagtttctgg ttgaggtcct
     3361 cgacgtcgag gttgtacagc gcacgcttgc gcaggtcgct gatggtgcgg accgacggga
     3421 tcacccgatg gccgcgggct tcgcccagca acgaaacgta atgcccgatg ccgaggtact
     3481 tgtagctgcg gcacaggttg atcacgtggg tccgttcgtc gccgccgacc ggctcgcgca
     3541 ggtactgcat cgcggtgacg acattgtccg acgggtaata ggagccccag tccgaggctt
     3601 tttcgacgac gatggccagt cgactcattg catccttccg ctacgctgca cgggtccgcc
     3661 actcccggcg aaccacattc gaatgtccgc gcagtgtcgg tatcctgcgc ggcaaacgca
     3721 agatggcatg cgcatgaccc ccgatcccgc ggcccggata cgcgaagctt cccccgacga
     3781 cctcgatgcg ctggtggcgc tggagcgcgt gaccttcgag tccgaccgca tcagccggcc
     3841 ccaatggcgg cgccacatcg gcagcgacag cgcgtgcgtg ctggtggccg ggccgcgcga
     3901 ccgcatcgac ggcgccgccg tcgtgttcca tcgccgcaac tcgcgtcggg cacgcctgta
     3961 ttcgctggcc gtcgcggcgg cttcgcgcgg gcgcggcctg gccaaggcgc tgctggcagc
     4021 cgcggaggcc gacgcccgcc ggcacggctg caacgtgctc gacctcgagg tccggaccga
     4081 caatcgcgcg gcgatcgcgc tctacgagca tcacggctat cggcgcacga cccgcctcca
     4141 gggattctac gaggacggcg ccgacggctg gcgattcacc aaggcactgg ccgacccatg
     4201 acacctgtca aggcggattg cattacatcc gggacgtgcg cgctgtccct cacgcgagca
     4261 tcatgaccct ccggattgcg ccgggtgccg cggctcgcac gcaacatgtc aagcaaactt
     4321 gacatgccag gcggccttgg ctaaggttgt aaagcaaact tgacaaggag tccgcaatga
     4381 aacgttcctt gacgctgggc cttctttccg ccgcgatcct tgcgatcacg gcccctgccg
     4441 caactgccgc ttcgcccatg gcgaccacca ccggcagccc ggccccggcg tcctcgtcca
     4501 gcgcccctgc cgttccggcc aaaccggtgg tcgagcgacc caaggcggac gcgacgatcg
     4561 atcgcatcat taccgcgatg aacaactcat cgacctgggg gcatcccgac ctgttcggcg
     4621 agttcgccgg catgcggctt ttctacgagg gccagttcaa ggccgccatg aaatatttcg
     4681 agtacggcgc ccgctacgcc gacaagttct cgcagctcag catcggcctg atgtacgaga
     4741 acggccgcgg cgtcgccaag gacccggtca ctgcctgcgc ctggatcgcc ctggccgcgg
     4801 aacgcaagta cccgacgtac atcgccaccc gtgaccgggt ctgcgaagcc ctcacccccg
     4861 cgcagcacga ccaggcggtg gcggtgctgg acaaactgct gccggaatac ggcgatgcgg
     4921 tggccaagcc gcgcatggtg cgggcgctgg aacgagccag gaccgaaatg accggctcgc
     4981 ggaccggctt cgacttcggg gccgaatcgg tgggtgtcat gaagggcggc tgcggcggtc
     5041 ccaccatcga aggcacctcc caggccggtt gcggcagcac ggatttctgg agcccatggc
     5101 gctgggatcc gaagaagtac ttccagatgc gggacgccca ttggcgtggc gtggtcaccg
     5161 tcggctcgct ggaaaaagag agccagacgc acgtgaaggc cgcgggcgcg ggaaagaatc
     5221 cggcgcaggc ggcgccggcg ggggcatcca gctcgggcca ctgaaccgcg tccccggcgc
     5281 gccccatata ctcgcaggat gttcctgatc gggattccga acatgcgccg tcacgtggtg
     5341 tccgcatcgc tgctgttgtt cggcgcagcg ctgctggcgg gctgcgggca gaagggaccg
     5401 ctgttcctgc cgaagcccgt ggcgccggcc gcgcctgcct cccctgctgc accggcgtct
     5461 tccgttgcag cacccgccgc gtccgtggcg cccgcaccgc cgcagccggt ggccggcgcc
     5521 acctccgccg cccctgcccg cagcggctga gccgcatcgc catgggcttg cgtttcacga
     5581 agatgcacgg actgggcaac gatttcgtgg tgctggacgc gcgcacggac gcgctgcaga
     5641 tcgaggccag tgtcatccag gccatgtccg atcggcacaa gggcatcggg ttcgaccagt
     5701 tgctggtgat cgagcgccca caggaccgaa cctgcgtcgc cgcctactcc atctgcaatg
     5761 ccgacggctc gcgtgccgaa caatgcggca acggtgcgcg ctgcatcggc gcgtggctgc
     5821 accgcgacgg cgtgctcgcg cccggcaccg aggccaggct ggaaagcccc gcaggcgtcg
     5881 tggcgatgcg gctggcggat gccgcgaccg tcgccgtcga catgggcgag ccggtgttcg
     5941 aacccgcgcg cattccattc gatgcacccg ccttcgccgc aacctatccg ctggagatcg
     6001 acggtgacca cctcgagatc gccgcggttt ccatgggcaa tccgcatgcc gtgatggaag
     6061 tcgccgatgt ggcggatccg cgcgtcgatc gcctggggcc gcggatcacc acgcatcccc
     6121 ggtttcccga gggagccaat gccggcttcg tgcagatcgt cgatccgcag acgatccgtc
     6181 tgcgcgtgca cgaacgcggc gctggctgga cgctcgcttg cggcagtggc gcatgcgcgg
     6241 cggtggccgc tctgcaccgg cgcggccgcg tcggcaaccg cgtattcgtc gaactgccgg
     6301 gcggcaccct cgagatcgcc tggcgcggca ttggccaccc gttgtggatg accggccccg
     6361 ccgcattcgt gttcgacggc gaatggctcg gatgattcca aggccacccg acgaaaaacc
     6421 attttgcttg catgttccgc ggattgcgtt cggtcgtccg aacatccaaa cttcgcggca
     6481 acttcacacg acactccacg catgtccgaa ttgaccctga agcaaagcct cgaggcaatg
     6541 gatgtcgccg aatacctgcg ccagcatccg cgcttcctgg aacactttcc ggacgtcgcc
     6601 gcggaactgg tgatgccgcg ggaacacggc ccggcggcat cgctcgcagc ctaccagctc
     6661 gaaaccctgc gtgcgcgcaa ccgggaactc aaccagcggc tggcggaact cgtcgaggtc
     6721 gccggcgaca acgagcagtt gatggtgcgc gtgcacaccc tcacggtcgg actgttgcga
     6781 gacgacaacc tcgccgatac ctgcaagagt ttcgtcgccg cgctccacga ggacttccac
     6841 accgacctcg ttcgcctggt gctgttccgt gcgggcgccg acctgccgca ggccggctgg
     6901 atggtcgcag tgccggaggg cccccgggca tggccggaat tcgccgagtt cctgtcgcgg
     6961 ggcgaaccgg cctgcgggcg cctcgcgccg gaaaagctcg agcggctgtt cggcggcgag
     7021 acagcccggg tgcaatcggc ggcgctgctc aggatcggcg gcctcggcat gctcgcgatc
     7081 gccagcgacg atcccaaccg cttccacccc ggcatcggca cgattttcct caagctgatc
     7141 gcgcaggcgc tggacgccgc gatctcgcgg ttcccggccg aatgagatga atggcgcgct
     7201 ggccgccgat gccgcgggat tcctcgaata cctgaaggtc gagcggcgct attcacccgg
     7261 cacgctgcgc aactacaccc acgcgctgga cgcgctgacc cgcttcgccg acggcctcgc
     7321 gatcgggaac tggagcgaac ttcgcggcga gcaactgcag accttcatcg cgtccgaaca
     7381 ccggcgcggg ctggaaccgc catcgctgcg ggcgatgctg tcggcgttcc gcagcttctt
     7441 ccggttcctc gcccgcgacg gcaggatcca gaacaatccg gccgccgggg tgcgctcgcc
     7501 caaggtgcgc cggaagctgc ccgaggtgct ggacgtcgat gaagcggcga cgctggtcga
     7561 ggtggccgca ggcgatccac tggcactgcg cgatcacgcg atgctggaac tcctgtattc
     7621 gagcgggctg cgggtttcgg aactgtgcgg ggtgcgctgg cgcgacctcg atgccgagca
     7681 aggcttgttg cgcgtgaccg gcaagggcaa caagacgcgg atcgtgccgg tcggacgcat
     7741 ggcgctgtcg gcgctgacgg cgcttcgcga agcacaaccg gcggacgccg acgaaccggt
     7801 cctgcgcggc cgcggcggac gtgccctgac cgccggcgcg gttcgtgccg cgctgaaacg
     7861 ccgggcgcgg caacaaggcg tctggaagcg cgtgtatccg cacctgctgc ggcattcatg
     7921 cgcctcgcac ctgctcgaat cctccggcaa cctgcgcgcc gtgcaggaac tcctgggcca
     7981 cgccgacatc ggcaccaccc agatctacac ccacctcgat ttccagcacc tcgccaaggt
     8041 gtacgacgcc gcgcatccgc gcgcgaagcg gaagcggggg acgggcgggt agcggggcgt
     8101 gaacggcgac gttgcggcag gcgggcggtt ttcgtcgttc ttgcgattcc cagtcacgtc
     8161 aagggcttcg tttttcccgc ccgctgcatc agaagcccgc caccggcgat gacgcgtcac
     8221 aggtcgtagc caagacgatc gatccacggc cgcagtaccg gcaacaccgg ctcgaacatt
     8281 tcccggtagt gcatccagcg ccccaccgcc gccttgttca acggctgcgt gacctgcgca
     8341 tagctggggg tcgcgatgaa ctgcttgcgt cgggcattgc ccgcgaaatc catcatggcc
     8401 gatgcatccg ccactgccag gaaatctccg agcctcgaga cggccgcggc cgggtcggcg
     8461 acgaccgatt cataccgcca ctccagcaca cgcggcttga acacttcgat gtgcccgcag
     8521 cactgttcga acgcttcggt atatccccgc gccagccgcg gcagcgcgga acacatcgac
     8581 ctgaacgccg gcgagcggaa cgcctgcatg ctgcaactca gcaacacgtc gcacggatgc
     8641 cggatgcaca gcacgatgca agcatgcggg aacagtcgca ggatcatcgg caggcacaac
     8701 atgttgagcg gattcttgtc caccagccgg cgctggccga ggtccggcag tgtccggcca
     8761 accaggcgtc cgtacaccga gcgcaattga tcggcttcca gttgcgtcag ggcggcgagg
     8821 tcggctgggt atttctgttc cgcgtgctcc atgcgctgga tcaactggta gatgtagccg
     8881 cgctcgtcca tcgaccggaa gtcggtgtgc gcgtcgagca tctgttccag caaggtggtg
     8941 cccgagcgag gaaatccgat cacgaatacc ggactccgcg cggtcgatgg gcccttcacc
     9001 ggtttccagg tggcgtatgc ccgcgcatcc accgaccgcg acaccatcgg caaaggctgg
     9061 ccgctcgctt ccgacagctc cggcgcggct tgtcgcacga tttccatttg cgcggcgtgc
     9121 gcgacttcga gtgcgctcca tgcctcgcga tattgcgctt gccggtcgca cgccgaagcc
     9181 aagccgaagg ccgcattcga tacaacctcc gggtcggcgg ctacgtcgag cacacgctga
     9241 aaaagcgcgg ccgcaacggc agggcgacct tcgcgcatcg ccatcaccgc atgcgcgcgc
     9301 cacacgtcga tgcgcgcgtc gatcccgcct gcccccaagg cgtcaagggc gtccagcggc
     9361 aactgttgca gttcgcaccg cgcctgatcg acccgattgt tccgttcgta caacgcgacg
     9421 cgctgcgcgg tgatgcgcag ccgcatggcg tcggcagccg gcccctccgg caggcgcgcc
     9481 tgcgccagta catcgagcgc gatctccagt cgacccagga tggacaaaat ggaagcgagg
     9541 accaacgcct gcgaagccgg ctgcggaggc cacgcgccgg cgccttccag cattgcctcc
     9601 tcgccctcca gatcgccaca aacgtggcag gcatgggccg cctccaaccg cgcctcgatg
     9661 aaccgcggcg ccagccgcgc cgcgtccagc agcacgccgc gcgcaagcgc ccagcgctgc
     9721 tgctggatgt acagcaggcc gaggttgtaa agcacgtcgg catcgccggg cgccagtgcc
     9781 cgcgccttcg aaagcgcgcg ttccgccacg gccaggtcgc ccgcttgtcg ggatgattcg
     9841 ccgagattgt tccaccacgc cgcgacatcc ggctggatgc tcgtcaatcg tccgaaggtt
     9901 tgcgcggccc gcgaataatc gcccgcctgc tgtcgtgcca tgcccagcag cgcgaggaac
     9961 gcttcgtcgt cggcctcgac gtcggacgcc tgcgccgcca gcaccaggac gcgttgcgtg
    10021 tcgcccgcct tccatgccgt ggccatggat tcgacgatgg gggtggccga agcaatcatg
    10081 cgaaagcgat cctccaggat ggcgccactc tagcggatgc aaaaagggca ccgctcgcgc
    10141 ggtgcccttg ccctgccgtg cagtcgcacc cgggtggcgg gcgttcagaa cttcagcgtc
    10201 gcgcgcgccc agtagtaggt gcccatcatg tcgtaggtgg cggaatcggt gttgccttgt
    10261 ccgttgtggt agatcagcgg cggacgtttg tcgaaaaggt tgtccacgcc gatatcgaaa
    10321 cgagtgtgga tgcccggcac tgccacgccg acctgtacgt cgttgtacac caccgaggcg
    10381 accggcaagc tcgcgccggt cgcgtaatcc gcgttgagcg cggtgacgcc gctgatgtaa
    10441 cgcgtgcgcc actgggcatc ccagttgccc cgatcccagc cgagggtggc cgtcgcgcgc
    10501 cagcgcgaga cgttgccgaa ctgcgcgttc caggttccgg ccaatttgtc gacggtggaa
    10561 ccgggcaacc cgggggtctt gttgttgttg aaggtggaga cgtagctggt ggccagaccc
    10621 aacttgaaat cacccggatc gaagccgccg aaatcgaagt gtggcagctt gtagttggcc
    10681 gtgaaatcga tgccgctggt actgagcgat ccgaggttca ccaccggcgt gtcgatgtag
    10741 aagacctgtc ccggctgctg ggtggtgttg tcgtagcgat ggatgaacgg gcagaacggg
    10801 ctcgcgccgt tgttgaagca cgcatcgacc acggtgcgac cgtcgatcgc caccagcgtg
    10861 tcgtacaggt agatgtgcca gaaatcgagg ctggtgttca ggccctgtgc ccagctcggg
    10921 ttatagacca ggccgaaatc caccgacttg ccgtgttccg gcttgagatt gccgcccacc
    10981 acgttcgccc ccgagtagta cgtgttgacc tgcgcggggc tgttgcccgc ccagtccggc
    11041 ggcacgtact ggcacgcgac gggatgctgc gcaagctccg cggcggacag gtgcgcgcag
    11101 gggtcgttca aggccggctg caccagcgac cggccatcgt agagctggtc gaggttcggg
    11161 gcccggaata cctgcgaaac cgtgccgcgc accaagaggt ccgggatcgg acgccactcg
    11221 atcgcgacct tgccgttggt ggtggagccc gcgctgttgt agtcggagta gcgcacaccc
    11281 acgtcgacgt tcaacgaata cgcaccgggc acctccttca gcagcggaat cagcgtctcc
    11341 gcgaaggcct ccttgacgct gtcgttgccg ctgccgggtg agccgcaggc ctcctgcgaa
    11401 atctggcaga agccggtcac gggatcgagc aaggcgagcg gatcgatcgt gtagttcatg
    11461 ctttgcttgc gatacaacgc gcccacggcc aattgcacgt cgccggcggg cagcgagaac
    11521 aggttgccat tggcgcccgc cgaaatctgc cgcaggatct cgcgcttccg gtagatcgca
    11581 tcgaccgcgc ccgcacccaa tgccgcgccg ttgttggcct ggtcgaaaat gttggcaccg
    11641 gcatcgatgg ccgcctgcaa cgcgggaatg tacagctcgt tctcgtcgtt ctgggtgcgc
    11701 gaggtatggc cgtaatcgac ggtgacattc cagttccagc tgctctggcc gagcgcgccg
    11761 cgcaaaccgg cgatcaactg gccggtgctg gtggtgtagg ggtggatgcg ggtacccacg
    11821 ccggtcaggc gcgtctgata cacgtagccg ctgttcggat cgccggggat gggattcggg
    11881 ctgaaggtga cgccgaacgg attgaacggc gcggtcgcgg gaatcaccag tccgtcaccg
    11941 gtacccaccg gaatggacgc gtcctggccg gccgattcgg tatggttgta gaaggcgtcg
    12001 acgaacgccg tgatgttgtc ggtcaggttg tagctgccga ccaggaaccc gtcggtgcgc
    12061 tgctgctgga cctggatgta gttgaaggcg gcgtagttga aggtgtcgct cgaagtgcgg
    12121 caacggtaat cgccgagcgt cgtgccatcg ccggcatccc gcgtgaccac gatgtggtcg
    12181 gtcgaatcgg gcgtgcatcc gtattgcgcg gcaaacgacc ccggcaattg catgcggccg
    12241 ttgggggtgg agctggatcc ggcgacgctg atctgcccat cggtcaggta caactggtgg
    12301 gccgcgaaat cacgccgagt gttgggcacc gcgtcgtatt tgttgtagtt gatgccgccg
    12361 acgatgttgt acctgtcgcc ggtgtggccc atggtcaggc tcagtccgtg ccgctggccg
    12421 tcgccgtgtc cggaaatgcc gccgttcgcg ctcacttcga cgccatcgaa atcatggcgc
    12481 aggagaatgt tgacgacacc acccacggca tcggacccgt aggtggtcga ggcaccctcg
    12541 gccagcacgt cgatccgctg caccatgttc tggggcacca ggttcatgtc ggcattcaac
    12601 atgcgctggc cgtcgatcag caccagcgtg cggccggtgc cgaggccgcg cagcgaaaca
    12661 cgcgaggccc cgtcgccgcc ttcggtcagg ggcgacgcga cgccgccgcc attggagttg
    12721 ttctgcggat tggtggcatt gccggcgacg ctgggcagtt gctgcaggat gtcaccgagg
    12781 gtgggtttgc ctgaactggt gatgttctgg cgatcgatga tggtgacggg actcgccgtt
    12841 tcggtatcca cgcgccggat caacgatccg gtgacgacca cggtctgcaa cgtcttgggt
    12901 tgactgttcg cctgatcctg gtcggcggga ttggccgtct ggctctggcc gttcggcggc
    12961 tgctgcgcct gggccatgcc ggtagccgcg agaagtccgg ccgtcagccc cagacgcaca
    13021 gcgagggtca agcgattcaa ttcgtggttc atacgttctc ccgggggaat ttttcgagat
    13081 gcatcaccgt gcccactgac cgtaccgagc cgcaaacccc atgcgatcag agcgcggttc
    13141 ggcacacccc tgtccgtcaa ccacggcgtc gttttaccac tacatggccg catatcgcca
    13201 tacccacgac ctgcggtacg cgggaattgc cgctccgaag tcccgcttcg cgatggggtc
    13261 aatccacgcc gccgtgcgtt tcgacatgcg cgaaggcctg ctctccagca aagagggatc
    13321 gcccgccggc gcgcgccccg cgagggacgc ttgccattca ggttcaatgc ccccacctac
    13381 tcgccgatcc ctttcaacac gaggcgtcca tggaatcctt ccatgccact accatcctgt
    13441 cggtgcgcaa gaacggcaag gccgtgctgg gcggcgacgg ccaggtcacc ctcggcaaca
    13501 ccgtggtcaa ggccaatgcc cggaagattc gccggctggg caagaacggt gacgtgctgg
    13561 ccggcttcgc aggcgcgacg gccgacgcct tcaccctgtt cgaacttttc gaggacaagc
    13621 tggccaagca cagcgggcag ttgacccgtg ccgcggtcga gatggccaag gaatggcgga
    13681 ccaaccggcg cctcggccgg ctggaggcca tgctggcggt ggtcgacaag gatgcctcgc
    13741 tgctgatttc cggcaacggc gacgtgctcg aacccgagca cggcctgatc gcgatcggct
    13801 cgggcggccc ctatgcgctg gccgcggccc gggcgctgat ggaaaacggc gatctcgacg
    13861 cgcggcagat cgtcgaaaag gccctcggca tcgccggcga catctgcatc tacacgaacc
    13921 acaacgtggc gatcgaggaa ttggacagcg ggaacaggga acagggaacg gggtgaagtc
    13981 ccatcacgct gcctcgccgc atactcactt tccgttttcc cattcccgtt actcaagcca
    14041 atgagcgaac tgactccccg cgaaatcgtc aacgaactcg accgcttcat catcggccag
    14101 cacaatgcca agcgcgcggt cgcgatcgcg ttgcgcgacc gttggcgccg gatgcagctc
    14161 gatccggcgt tccgcaacga ggtgacgccc aagaacatcc tgatgatcgg gcccacaggc
    14221 gtgggcaaga ccgaaatcgc acggcggctc gcgacgctcg cgaaggcgcc gttcgtgaag
    14281 atcgaagcga ccaagttcac cgaggtcggc tatgtcggca aggacgtcga ttcgatcgtg
    14341 cgcgacctcg cggatgtcgc ctacaagctg acccgcgacc aggccaagga gcgggtgcgc
    14401 tcgcaggccg aggagcacgc cgacgagcgc atcctcgacg cactgctgcc gcgccgcgaa
    14461 gcgggcggcg gctgggacca gccggccgat gccgaaaccg cacgcatcga atccgatgcg
    14521 tccacccgca ccaagctgcg caagcaactg cgcgacggcg cgctggacga gcgcgagatc
    14581 gaactcgagt tttcgatgaa cgtcggcgtc cagatcatgg cgcccccggg catggaggag
    14641 atgtcgcagc agttgagttc gatgttccag cacctcgggg gcggcaagac ccagaagcgc
    14701 aagctgaaga tcaaggccgc gcggccgctg ctgatcgacg aggaagccgg caagctgcta
    14761 aacgacgacg agctgcgcgc gcaggcgatc gaaaactgcg agcagaacgg catcgtgttc
    14821 atcgacgaga tcgacaaggt cgcgcagcgc agcgactggg gcaccaccgg tgcgggcgtg
    14881 tcgcgcgaag gcgtgcaacg cgacttgctg ccgctggtgg aaggctcgac cgtctccacc
    14941 aagtacgggc cggtgaagac cgaccacgtg ctgttcatcg cctcgggcgc gttctcgatg
    15001 gccaagccgt ccgacctgat ccccgagctg cagggacgcc tgccgatccg ggtcgagctg
    15061 tcggcgctgt cggtggacga tttcaagcgg atcctcgagg agccgaacaa cgcactgacc
    15121 aaacagtacg ccgcgatgct gaagacggag ggcgtgacgc tcgaattcaa ggacgaaggc
    15181 atcgcgcggc tggccgaagt ggcgttcgac gtgaacgaaa gcaccgagaa catcggcgcc
    15241 cgccggctgc agaccgtgat ggagcggctg ctggagacga tttcgttcga agccgcggac
    15301 aaaagtggcg aaacctacgt gatcgacgcc gaatacgtcg acaaaaacct caaagacctc
    15361 gcctccgacg aggatttgtc gcgctacatc ctgtaaggag agcttctgag taccgggtca
    15421 gggtattccc cggccggcca gctacaatga caccatgggc aaggttctga aattcgagtc
    15481 ggagagtccg cgcccgaagc tccgtggggc gctgcagcgg ggagaactgg tgcgggtctg
    15541 gcgcagcggc ctcgaggacg gcagtttctg cgggtacctg gccggcatcg gccgcgagtg
    15601 gttgctgatg tgggtggtgg gcgaccagat cggcttcgat ggcatgtacg cgatgcgcct
    15661 cgccgacatc accgaggtcg aagcccccga cagccaccac gtcttcatgg aaaaggcgct
    15721 ggcggttcgc aacatccatc cgcgcctgcc ccacaacttc ccgctcgacg acatcaccca
    15781 ggtcgtgcgt gccgccgccg aatacgccgc ggtgatgtgc gtgcacgtgg acaccgagga
    15841 cgagcacgag gtctgctaca tcgggcggcc cctcggcatc gaggatgacg gtttcaccct
    15901 gcaggaaatc agccccgacg ccgaatggct acgcgagccg tcgttcttcg gctgggacga
    15961 gatctcgacc gtgagcttcg atgaacctta cgcgcaggtc ctgctggacg tggccggtca
    16021 gccgccgcgc atcgatttcg caggatcggg attcggaagc gcaaattaag gtgcgatcgt
    16081 ccttgatcgc tgcatcgctc aaccttccgg cgcctgccgg catttcgaag tcaggaacac
    16141 cagaacggcc atccatggcc tgacttttca caaaagcggc ttcgcatggg gtgctgggaa
    16201 aagcctcgtg accccctttt cgtagttgtc gctggttggg cgggcgatgc ccgccccaca
    16261 ggcgcgcgtt cgctgacaca tatcagcagg catcggacac gtccctgtaa aattggttgc
    16321 atgccggccg ttcccaaacc caccgacatc acactgcaca agaaatcgcg cacgctggaa
    16381 gtcgcgttcg actccggcga acgcttcgtg atgccctgcg aatacctgcg ggtccattcg
    16441 ccttccgccg aagtgcaagg ccacggcccc ggggaacggg tgctggtccc cggcaagcgt
    16501 gacgtcggca tcagcgcgat cgaaccggtc ggcaactacg cggtgctgct gcgcttctcc
    16561 gacggccacg ccaccggcat cttttcgtgg gagaccctct acgacctcgg catcaaccag
    16621 gaaacgaact ggcagtcgta cctggatgcg ctcgagaaga accagctctc gcgcgaaccg
    16681 aagtcgccct acgggcaccc ggtccccatg cgcggcccaa agccgcggta ggtcggtcgg
    16741 gttgagccgg cttcatcggc gaagcccgac acgattgaca tcgaaatatt gggcttcccc
    16801 ggaggacctc cggtccagcc gcctacgtgg aagctttcgc gagcaccgcg gcgcgccgtt
    16861 tcaaactttc cttcggccag cactgctcgc gatcgtaata cccgggcatc gcgggcacgc
    16921 catcgggcag gatcacccac ggctgcttgg atgtcgtgaa gatgtggatg tcgggcggga
    16981 agcggtccgg atcgtccagc gtgccgaccc ggatgaaggt gaacagcgga cccgcaccgg
    17041 cgtagcggct ccacagggcg acccgacata ccggacaacg ggcgatgtcc tggccgctgc
    17101 cgctcgccga tggcgtcatc accatctctg gttccccgtg caaccgctcg acgctttcgg
    17161 tctcgatcag ggcgttcagc acgaaggcac tgccggtctc gcgctggcac cagcgacagt
    17221 ggcagcaatg cacgaacagc ggcgtgcggg tcagccggta acggatgaaa cggcagtcgc
    17281 agccgccttc gaaggtttcg gattcttcgc tcacaagttc ctcgcgagcc ggccatcatt
    17341 tcctgacgtc attccggacg cgatgcagcg catcgcgatc cggaatccgg tgtctttccg
    17401 gcaacttgaa aggcactgga ttccgggttc cgtttcccga tccaggcggg aaacggcccc
    17461 gaatgacgaa ttgggttact ccgtcgcgaa cagcgcgttc aagtcttcct catcgaaggc
    17521 caacttgtcg cgcgtcccgc cgccttcgag cacggcctgc gcgagctccg ccttgcgtgc
    17581 cttcaggtcc tcgatccgtt cctcgacggt gtccgcgcag atcaggcggt agacgaacac
    17641 cggcttgtcc tgcccgatcc ggtgggcacg atcggaggct tgggtttcgg cagccggatt
    17701 ccaccaaggg tcgtaatgga tcacggtgtc ggcggcggtg aggttgaggc cgacaccgcc
    17761 ggccttcagc gacagcagga acagcggcac ctcgccttcc tggaacttgc gtacgggttc
    17821 ggcgcggtcg cgggtatcgc cggtcagcgt cacgtactgc aggtggcggc ggttcaattc
    17881 gcgcgcgatc agcgccagca tctcggtgaa ctggctgaac agcagcaccc gccggccttc
    17941 gctcagcagt gccggcagca tctccatcag gagttcaagc ttggccgact cgcgaatgcc
    18001 ccgcgccgat tcgagcttca cgaggcgcgg atcgcagcag gtctggcgga gcttcagcaa
    18061 tgcgtcgagc acgatgatgc cgctgtgctc gatgccgcgc tgtcggatca cctcgcgtac
    18121 ctcgtcgccc agtgacacgc gcagcgagtc gtacagctcc cgctgcttgc ccgacagcac
    18181 cacccgcctc gcgatctcgg tcttctcggg aagttcggag accacctgcg ccttggtgcg
    18241 ccgcaggatg aagggcgcga tccggtggtt gagg
//
LOCUS       DASSNH010000288         5961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_193364, whole genome shotgun sequence.
ACCESSION   DASSNH010000288 DASSNH010000000
VERSION     DASSNH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193364"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /gene="pmbA"
                     /locus_tag="VFK00_10615"
     CDS             complement(<1..1311)
                     /gene="pmbA"
                     /locus_tag="VFK00_10615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HET7593441.1"
                     /translation="MNPDIVTADDSIAELDRLAELAEDVISRARAAGASQAEVSASVD
                     TGLSVSVRLGEVETVERTRDRGFGLTVYFGQRKGNASTADLKPGSIEATIAQACAIAR
                     YTEPDPCAGLADADRMATDFPDLDLWHPWNLDIDHAIEIGREVEAAGRTESRITNSEG
                     ASVQHGGGLGVYANSHGFVGRERGTHHSISCSLIAGAGDAMQRDGWYEYVRDPALFPD
                     FARIGRTAAERAVARLDARRISTRECPVLFAPEVARSLLGHLIGAVSGGSLYRRASFL
                     LDHAGKPVLPGFVDLLEEPLLPGGHASTAFDAEGVGTRASALVEGGVLQRYVLGSYSA
                     RKLGLPTTGNAGGVHNLVVPPGSVDGAQQDFAGLLERMGRGLVVTELLGQGVSIVTGD
                     YSRGVAGFWVENGKIAHPVQEVTVAGNLRDMFMGVQAIGTDVDPR"
     gene            1344..1880
                     /gene="yjgA"
                     /locus_tag="VFK00_10620"
     CDS             1344..1880
                     /gene="yjgA"
                     /locus_tag="VFK00_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HET7593442.1"
                     /translation="MATHDSSDEADHERPSRSARRRETLDVLAFAKQLSELPPARIGK
                     LELPDDVREELAQVQRTPSHIAHKRQLAHLAKVMRAHDDAEFASARAVLDNDRAAGAR
                     EAAELHRTEALRERLLGDEADAALTDFIATHPGADRQRLRALIRQARRERGSGKPPRA
                     QRELFRLLRNSEKLPDAD"
     gene            complement(1961..3403)
                     /gene="tldD"
                     /locus_tag="VFK00_10625"
     CDS             complement(1961..3403)
                     /gene="tldD"
                     /locus_tag="VFK00_10625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HET7593443.1"
                     /translation="MMDSPLSLAERNLLAPGGLSTNDLERVFARVMAPSIDAADLYFQ
                     HSRSESWVLEDGIVRDGSHSIEQGVGVRAISGEKTGFAYSDDIVLPQLLEAAGAARAI
                     AREGNGSGHPLAVRGTRALYPADDPIEGLDNAAKIALLRELDALARSLDSRIRQVIVS
                     LAATHDSVLVAAADGTLAADVRPLTRINVRVIAEDGGRREVGYAGGGGRCAYRELLDS
                     GRAQAWAREAVRQALVNLEAVDAPAGTMDVVLGPGWPGVLLHEAIGHGLEGDFNRKGA
                     SAFAGRMGDKVAADGVTVVDDGTLPGRRGSLSIDDEGTATNCTTLIENGRLVGYMQDK
                     HNARLMGMAPTGNGRRESFAHLPMPRMTNTYMLAGERDPGEIIASVERGLYAVNFGGG
                     QVDITNGKFVFSANEAYLIEHGKVTRPVKGATLIGSGPDVLTRVAMVGNDLKLDDGVG
                     VCGKDGQSVPVGVGMPTTKIAAMTVGGTAS"
     gene            complement(3447..>5961)
                     /locus_tag="VFK00_10630"
     CDS             complement(3447..>5961)
                     /locus_tag="VFK00_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET7593444.1"
                     /translation="AGKITSARVLMRGDLDDWPFLDHQGRFEATATVADAAFDFSNEW
                     PQATGVDAAVDFVDNHMAIVATHVSVQGVTATHAVATIPDLSHGVLNLEVQGGGRGPQ
                     LLDFVRHTPLATDAAEALQGLTVGGRANFGVKLSIPLNDPKAFKLDGKVDLAHADVTA
                     QKWKVAIEDLGGQMLIDSTGFHAPKLTGIFRGAPATLSMAVGGSVSDPLDIVEASLDT
                     RASAQTLVQGYPDLAPLIARVSGAARFDIGVRLVRGDGGAPPVPILDVQSSLAGLALD
                     FPAPLDKPAAATMPLKLTLQLPPEGAPLTVSLGDVLQVRGRLADPSHNRPAALAMRFG
                     SGMPERLPVQGLVVDGHAQRLDVSGWIQQALGGGPGAAFPQLDRADLSADQAVVFGTD
                     LGALRFDYAAGAGSDTIRFDGTAVRGTVGLPTTDLMTRGITANFEYLHWPQPPKPKEP
                     KKPATKPEPPQVTSPIAPTAIPPLHVTLGDLRLGDMKLGAIAFESAPTAQGMHVAKFD
                     AKGDGFTLHGSGDWDGNMAFSRSRFAVDIAARDFGKTLAAFGFSGLLAGGQDTHIHID
                     GAWPGSPSSFSLAWMSGKLDMKVGEGRILAVKPGLGRVLGLLSVRELPSRLMLHFGDV
                     FKSGFGFDGASGTFALRDGSAYTDDMLIDAPAARIAIGGRAGIRAHDFDLTINVTPHL
                     GGTLPVVGAVIGGPVGAAAGLVVQGLLGKGINKAAGSIYRVTGTWDEPKITTVKEAEP
                     PPAGSSAPLPAASSPASAPIPASVGPAPAAAGSAAPAPAATIPPAPASAATTAPAPPL
                     TTTPAPASTTIPAPGGGPARAPSAGVAAPAPAVSKLADH"
BASE COUNT          933 a         2109 c         2004 g          915 t
ORIGIN      
        1 gcgcggatcc acgtcggtcc cgatggcctg cacgcccatg aacatgtcgc gcaggttgcc
       61 ggcgaccgtc acttcctgca ccggatgggc gatcttgccg ttctcgaccc agaatcccgc
      121 caccccgcgc gaatagtcgc cggtcacgat cgagacaccc tgccccagta gctcggtcac
      181 caccagtccg cggcccatcc gctcgagcaa tcccgcgaag tcctgttggg cgccgtcgac
      241 ggagccgggc ggcaccacca ggttgtgcac gccgccggcg ttgccggtgg tcggcaagcc
      301 gagcttgcgc gccgaatagc tgccgagcac gtaccgctgc aataccccgc cttcgaccaa
      361 cgccgacgcg cgggtgccga cgccctcggc atcgaaggcc gtcgaggcgt ggccgcctgg
      421 cagcaacggc tcctccagaa gatcgacgaa tccgggcagc acgggcttgc cggcgtggtc
      481 gagcaggaaa ctcgcgcgcc ggtacaacga accgccgctc acggccccga tcagatgccc
      541 caacaacgaa cgcgcgactt ccggcgcaaa cagcaccggg cattcgcgcg tggaaatcct
      601 gcgcgcgtcc agccgcgcca ccgcgcgttc ggccgcggtc ctgccgatgc gggcgaagtc
      661 gggaaacagc gcggggtcgc gaacgtactc gtaccagccg tctcgctgca tcgcgtcgcc
      721 cgcgccggcg atcagcgaac aggaaattga atggtgggtg ccgcgttccc ggccgacgaa
      781 cccatgcgag ttcgcgtaca cgcccagccc gccgccgtgc tgtacgctcg cgccttccga
      841 attggtgatc cgtgattcgg tgcggcctgc cgcctcgact tcgcggccga tctcgatcgc
      901 gtggtcgatg tccaggttcc acggatgcca caggtcgaga tcggggaagt cggttgccat
      961 gcgatcggca tccgccagtc cggcgcaggg atcgggttcg gtgtagcggg cgatcgcaca
     1021 ggcctgcgcg atggtcgcct cgatcgaccc gggtttcagg tcggcggtcg aggcgttccc
     1081 cttgcgctgc ccgaagtaca ccgtgagccc gaagccgcgg tcgcgcgtgc gttccacggt
     1141 ttcgacctcg ccgagtcgca cgctcaccga aaggccggtg tcgacgctcg cggaaacctc
     1201 cgcctgtgat gcccctgcgg cccgcgccct gctgatcacg tcctcggcca gttcggccag
     1261 acgatcgagc tcggcgatgc tgtcgtcggc tgtcacaatg tccgggttca cattcgattc
     1321 ctgttcaaga aggttcggcc cgcatggcca ctcacgattc aagcgacgag gcagaccacg
     1381 aacgccccag ccgcagcgcg cgacggcgcg aaacgctcga cgtgctggcg ttcgcgaagc
     1441 agttgtcgga acttccgccg gcacggatcg gcaagctgga attgcccgac gacgtgcgcg
     1501 aggaactcgc gcaggtgcag cgcacgccct cgcacatcgc gcacaagcgc cagctcgcgc
     1561 acctcgccaa ggtgatgcgc gcgcacgacg acgccgagtt cgcgtcggcc agggccgtcc
     1621 tggacaacga tcgcgccgcc ggcgcgcgcg aagccgcgga gctgcaccgg accgaagccc
     1681 tgcgcgaaag gctgctgggc gacgaagcgg atgcggcact gacggatttc atcgccacgc
     1741 acccgggcgc cgatcgccag cgattgcgcg cgttgatccg ccaggcgcgg cgcgagcgcg
     1801 gatcgggaaa gccgccgcgc gcgcagcggg agttgttccg cttgctgcgc aacagcgaaa
     1861 agcttcccga cgcggattga cgcggatgaa agcaagtccg ttcttgctct tgtccgcgtt
     1921 tatcggctct tgtccgtgcc aaaaatgctc ccgctctggt tcatgacgcg gttccgccga
     1981 cagtcatcgc ggcgatcttg gtggtcggca ttcccacgcc gaccggtacg ctctggccat
     2041 ccttgccgca cacgccgacg ccgtcgtcca gcttcaggtc gttgccgacc atcgcgacgc
     2101 gcgtgagcac gtccgggccc gatccgatca gggtcgcgcc cttgaccggc cgcgtcacct
     2161 tgccgtgttc gatcaggtaa gcctcattgg ccgagaacac gaacttgcca ttggtgatgt
     2221 caacctggcc gccaccgaag ttgaccgcgt acaagccgcg ctcgaccgac gcgatgatct
     2281 cgcccgggtc gcgctcgccg gccagcatgt aggtattggt catccgcggc atcggcaggt
     2341 gcgcgaacga ttctcggcgg ccgttgccgg tcggcgccat gcccatcagg cgggcgttgt
     2401 gcttgtcctg catgtagccg accagccgcc cgttttcgat cagcgtggtg cagttcgtgg
     2461 cagtgccttc gtcgtcgatc gacaaggagc cacgtcgtcc cggcagggtg ccgtcgtcga
     2521 cgaccgtgac accgtctgcc gcaaccttgt cgcccatcct tccggcgaac gccgacgcgc
     2581 ccttgcggtt gaaatcgccc tccagtccgt gcccgattgc ctcgtgcagc agcacgccgg
     2641 gccagcccgg acccagcacg acgtccatgg tgcctgcggg cgcatccacc gcttcgaggt
     2701 tcaccagcgc ctgccgcacc gcctcccgtg cccacgcttg cgcgcggccg gagtcgagca
     2761 gttcgcggta cgcgcatcgc ccgccgccgc cggcgtatcc cacctcgcgg cggccgccgt
     2821 cctcggcgat cacgcgcacg ttgatgcggg tcagcggccg cacgtccgcg gccagcgtgc
     2881 catcggcggc cgcgaccagc accgaatcgt gcgtcgcggc caggctgacg atcacctgcc
     2941 tgatgcgcga atcgaggctg cgcgcgagcg catcgagctc gcgcagcagc gcgatcttgg
     3001 ccgcgttgtc cagcccttcg atcggatcgt cggccgggta caacgcgcgt gtgccccgca
     3061 ccgcgagcgg atggcccgag ccattgcctt cgcgcgcgat cgcacgcgcc gccccggccg
     3121 cttccagcaa ctgcggaagc acgatgtcgt cggaatacgc aaagccggtc ttttcgccgg
     3181 agatcgcgcg cacgcccacg ccctgctcga tcgaatggct gccatcgcgg acgatgccat
     3241 cctcgaggac ccaggattcg gaccgcgagt gctggaaata aagatccgcc gcgtcgatcg
     3301 agggcgccat gacccgcgca aaaacgcgct ccaggtcgtt cgtggacagc ccgccgggcg
     3361 cgagcaggtt gcgttcggca agcgacagcg gtgaatccat caggagcaat ccggcagcat
     3421 cacaatccac caacgtaagg gcggcttcag tgatccgcaa gcttggagac ggccggtgca
     3481 ggggctgcga cgccagcgga aggggctcgc gcggggccgc caccgggcgc agggatcgtg
     3541 gtggacgcgg gcgccggagt ggttgtcaga gggggcgcgg gagctgtggt cgcagccgaa
     3601 gccggggccg gtgggatcgt ggcagcgggc gcaggcgccg cactgcctgc cgccgcaggc
     3661 gctgggccga ccgatgccgg tatcggcgcg gacgccggcg atgacgccgc cggtagcggc
     3721 gcactcgacc ctgccggggg cggttcggcc tccttgaccg tggtgatctt cggctcgtcc
     3781 catgttccgg tgacgcggta gatgctgccg gcggccttgt tgatgccctt gccgagcagc
     3841 ccttgcacca ccaatccagc ggccgcgccg accggtccgc cgatcaccgc cccgaccacg
     3901 ggcagggtcc cgcccagatg tggcgtgacg ttgatcgtca ggtcgaaatc gtgggcacgg
     3961 atgccggccc ggccgcctat ggcgatccgt gccgccggcg cgtcgatcag catgtcgtcg
     4021 gtgtaggcgc tgccgtcccg gagggcgaag gtgccagagg ctccgtcgaa cccgaacccc
     4081 gacttgaaca cgtcgccgaa gtgcagcatc agccggctcg gcaactcgcg caccgacagg
     4141 agacccagca cgcgtccgag cccgggcttg acggcgagga tgcgtccctc gcccaccttc
     4201 atgtcgagct tgccggacat ccatgccagc gaaaacgacg acggcgagcc cggccaggcg
     4261 ccgtcgatgt ggatgtgggt gtcctgcccg cccgccagca gaccgctgaa gccgaacgcg
     4321 gccagcgtct tgccgaaatc gcgcgccgcg atgtcgaccg cgaatcgcga ccggctgaaa
     4381 gccatgtttc cgtcccagtc gccgctgccg tggagggtaa agccgtcgcc cttggcatcg
     4441 aatttcgcga cgtgcatgcc ttgcgcggtc ggcgcgctct cgaacgcgat ggcgccaagc
     4501 ttcatgtcgc cgagccgaag gtcgccgagc gtcacgtgca ggggcgggat ggccgtgggc
     4561 gcgatcggcg acgtcacctg cggcggctcc ggcttcgtcg cgggtttctt cggctccttg
     4621 ggtttgggcg gttgcggcca gtgcaggtac tcgaagtttg ccgtgatgcc gcgggtcatc
     4681 aggtcggtgg tcggcaagcc gaccgtgccc cgcacggccg ttccgtcgaa gcggatggtg
     4741 tcgcttccgg cacccgccgc gtaatcgaaa cgcagggcgc cgagatcggt cccgaacacc
     4801 accgcctggt ccgccgaaag atcggcccgg tcgagttgcg gaaacgcggc gcccggcccg
     4861 ccgcccagcg cctgctggat ccagccgctg acgtcgagcc gctgcgcgtg cccatcgacc
     4921 accagcccct gcaccggaag ccgttccggc attccgctgc cgaagcgcat cgcaagcgca
     4981 gcaggacggt tgtgtgacgg gtcggcgagg cgtccacgca cctgcaacac gtcgccgagc
     5041 gacaccgtca gcggcgcgcc ctctggcgga agctgcaagg tcagcttcaa cggcatcgtg
     5101 gcggcggcgg gcttgtcgag cggcgcgggg aaatcgagcg cgaggccggc caggctcgac
     5161 tggacgtcga ggatcgggac ggggggcgca ccgccatcgc cacgcaccag ccgcacgccg
     5221 atgtcgaaac gagccgcacc gctgacgcgg gcgatcagcg gcgcaaggtc ggggtagcct
     5281 tgcaccagtg tctgcgccga cgcgcgcgtg tcgagcgacg cctccacgat gtcgaggggg
     5341 tcgctcaccg atccgcccac agccatcgac agggtggcgg gcgcgccgcg gaagatcccg
     5401 gtcagcttcg gggcatggaa ccccgtgctg tcgatcaaca tctggccacc caggtcctct
     5461 atcgcgactt tccacttctg cgcggtgacg tcggcgtggg cgagatcgac cttgccgtcc
     5521 agcttgaaag ccttcggatc gttcagcgga atcgacaact tgaccccgaa gtttgccctg
     5581 ccgccgaccg tgagcccctg cagcgcctcc gcggcgtcgg tggcgagcgg cgtatgccgg
     5641 acgaaatcga gcagctgcgg accgcgtccg ccaccctgga cctccaggtt cagcaccccg
     5701 tgcgacaggt cggggatcgt ggccaccgcg tgggtcgcgg tgacaccctg cacgctcaca
     5761 tgcgtcgcga cgatcgccat gtggttgtcg acgaaatcga ccgccgcgtc gacaccggtc
     5821 gcctgcggcc attcgttgga aaagtcgaac gccgcatccg ccaccgtagc ggtggcttcg
     5881 aagcggccct gatggtcgag gaagggccag tcgtccaggt cgccgcgcat caggacgcgc
     5941 gcggaggtga tcttccccgc g
//
LOCUS       DASSNH010000289        10425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1319262, whole genome shotgun sequence.
ACCESSION   DASSNH010000289 DASSNH010000000
VERSION     DASSNH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319262"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1464
                     /locus_tag="VFK00_10635"
     CDS             19..1464
                     /locus_tag="VFK00_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7593445.1"
                     /translation="MDAGKVGSFISKLWDDEVIPQLVDYIRIPNKSPMYDRDWVAHGY
                     MDAAVALMEKWARSKLELLPGATLEVVRLEGRTPLIFIDVPASGGEARADDCVLLYGH
                     LDKQPEMTGWGEGLGPWTPVLKGDRLYGRGGADDGYAIFGSLSALLALHEQGVPHARC
                     VVMIEACEESGSYDLPYYVDHLAPRIGEPSLVVCLDSGCANYDQLWLTTSLRGMTGGE
                     LTVEVLTEGVHSGDASGVVPDSFRIVRHLLSRLEDPATGTIVPKQLYVEVPPERVEQA
                     KQCADILGDDVYAKFPWIDSMQPASDDRVELILNRTWRPQLAITGADGLPSLQNAGNV
                     LRPGTALKLSLRVPPTLNGSRAGDFVKTLLESDPPYGAKVTFKVEKDGSGWNAPQLSD
                     WLSSAVSQASEDAFGAPAAYMGEGGSIPFMGMLGEKFPKAQFLITGVLGPHSNAHGPN
                     EFLHIPTGKKVSAVVAGVVAAHALASAAGQT"
     gene            1486..1917
                     /locus_tag="VFK00_10640"
     CDS             1486..1917
                     /locus_tag="VFK00_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7593446.1"
                     /translation="MASIRAATAADAGDLAVLCGQLGYPADPATVARRLADIAECRSG
                     AVLVAEADDGAVVGWAEASLQRHLVHDPRVMLAGLVVAEGARNQGVGVALLGAVEAWS
                     REQGAAELIVRSNIVRERAHRFYLREGFAEKKRQALFLKPL"
     gene            1967..2626
                     /locus_tag="VFK00_10645"
     CDS             1967..2626
                     /locus_tag="VFK00_10645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7593447.1"
                     /translation="MMYDLPPVTRALLIANVAIFLLEQIPSLQPLLLVYGALWPVGAA
                     HPMVNGSGQVVLVGFHFWQLVTYAFLHGGWGHIFFNMFALWMFGGPIEHLLGARHYTF
                     YYFFCAVIAAAAHLLVAQFFTHGFYPTLGASGAIFGLLVAFGVMYPRMKMFLLFFPVP
                     MPAWVFVLGYIVLELFFGVSGYESGVAHFAHLGGAVGGFALLMYWRGKLPFKPKRILT
                     R"
     gene            complement(2725..3327)
                     /locus_tag="VFK00_10650"
     CDS             complement(2725..3327)
                     /locus_tag="VFK00_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593448.1"
                     /translation="MRHWKAWLLLALAVWAGWTWWGNRPVARAAGVLAPDEPVQVDFD
                     APQPPIPFEDATLRPLARFSLTARVLSREDYRLDAEAALSPTDLAVGWGAMSDTAVLR
                     DIDISQSGRFYYWQVQSFPIPRREIETHSANMHMIPANALVARALRRVRAGDIVALDG
                     FLVEADKPGGWRWHSSMTRTDTGAGACEVVYVRSLQIEPR"
     gene            complement(3362..3943)
                     /locus_tag="VFK00_10655"
     CDS             complement(3362..3943)
                     /locus_tag="VFK00_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HET7593449.1"
                     /translation="MKPLAQDDLLSLEQYARERADFRTRVLAHKRDRKVRVGEHVTLL
                     FEDRLTIQYQVQEMLRTERIFEPEGIAEELAAYNPLIPDGANLKCTMLIEYDDVEVRK
                     RELLRLRNVEHDVQLVVGGHPPVTAIPDEDLPRSDEQKTAAVHFLRFELEVGAVADFK
                     AGKPVAVRIEHPNYRAEATLDEAQRKALTADFD"
     gene            complement(4004..5347)
                     /locus_tag="VFK00_10660"
     CDS             complement(4004..5347)
                     /locus_tag="VFK00_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET7593450.1"
                     /translation="MSSDAAGTREGNLEAPTRHPLGQDDPVFRDQKALEKEMERIFDI
                     CHGCRRCVSLCHAFPTLFDLIDESETMEIDGVDKADYGKVVEQCYLCDLCYQTKCPYV
                     PPHPWNVDFPHLMLRAKAFEFERDGASLGDRFLSNTTAVGRITARPVVAETVNALNRS
                     KPARQLLDKTLHVHPDAEVPEYKHPRARKKLRKLDATGDATPAGRTRGKIAIFATCYC
                     DSVLPAVVDDLAAVFLHNDIPVKLVSKEVCCGMPKLELGDLASVKKYKDQNIPVLAHA
                     VRDGFDLTAAIPSCVLMFKQELPLMFPDDEDVRSVQRAFFDPFEYLAERHAAGLLKTD
                     FKASLGKLAWHVPCHQRVQKIGPKTRDILSLVPDTEIVTIERCSGHDGTYGVKTRTYE
                     LSRKIARPVERRITESGAVMSTSDCPMAGAHLAHGLQNQPPQQHPVSLLRKAYGI"
     gene            complement(5665..6084)
                     /locus_tag="VFK00_10665"
     CDS             complement(5665..6084)
                     /locus_tag="VFK00_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET7593451.1"
                     /translation="MANLKGSKTEQNLKDAFAGESMANRRYLYFASKADVEGYNDVSA
                     VFRSTAEGETGHAHGHMEYLEAVGDPATGLPFGETHANLKSAIAGETHEYTDMYPGMA
                     KSARDEGFEEIADWFETLAKAERSHANRFTKALNELG"
     gene            complement(6217..6630)
                     /gene="rnk"
                     /locus_tag="VFK00_10670"
     CDS             complement(6217..6630)
                     /gene="rnk"
                     /locus_tag="VFK00_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HET7593452.1"
                     /translation="MDTRTPITLSRLDVERIEALLEAPALRDSPVAKKLHEEFDRAEI
                     VEPAKMPHDVVSMNSSADCVDESSNRHHTLTLVYPKDADADAGKISVLAPVGSALLGL
                     RVGQSIDWPGNDGRTLKLKVTAIHYQPEAAGDLHR"
     gene            complement(6632..7609)
                     /gene="oxyR"
                     /locus_tag="VFK00_10675"
     CDS             complement(6632..7609)
                     /gene="oxyR"
                     /locus_tag="VFK00_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HET7593453.1"
                     /translation="MNLRDLQYLVALADHKHFGRAAEASFVSQPTLSTQIKKLEDELG
                     VTLVERSPRKVLLTEVGKQIAARAREVLNEVEQIKRVARRDHDPASGTLRLGIFPTLG
                     PYLLPHVLGTIRERFPRLELLLVEEKTPELVQRLREGKLDAAILAQPVHDETLHAEFL
                     FEEPFLLAAPEAHPLAGRRKLKLADLEGQRLLLLEDGHCLRDQALEVCRMSGADERQG
                     FRATSLETLRQMVAADVGITLLPVLAVQPPIAHTANVHLTPFGSHAPSRRIAMYWRRS
                     SAMDGFLRELSKVFKNLPRRLLDPATLPAPPIVKSTAARYVGGKNHAKA"
     gene            7783..8322
                     /locus_tag="VFK00_10680"
     CDS             7783..8322
                     /locus_tag="VFK00_10680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7593454.1"
                     /translation="MLTVGNNFPHFNLKATVSIESLDKAFTDIDNSTYKGKWTCVFFY
                     PKDFTFVCPTEIKGFADLDAQFKDRDCQILAASTDSEFVHMAWRKDHKDLRDLPFPML
                     ADINKDLTRALGILDEEGVAKRAAFLVDPEGVIRFVYVTEGSVGRNPAEVLRVLDALQ
                     TDELCPCNWNKGEETLKAA"
     gene            8465..9028
                     /locus_tag="VFK00_10685"
     CDS             8465..9028
                     /locus_tag="VFK00_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7593455.1"
                     /translation="MNLDQLKSQLPAYAKDLRLNLESVLGEGGAPGLGQKQIAMVALA
                     SAIASRHAPLTEAITQFASQHAGEKDLEGARTAAALMGMTNIYYRFLHLVENDEYGTL
                     RAGLRMNAMASPGCDKVDFDLASVAVSAINGCGSCVASHERTLRKHDVSAQAVQSAAR
                     IAAVIHAVAVVLEQQEAAATASAAEAA"
     gene            complement(9033..9941)
                     /locus_tag="VFK00_10690"
     CDS             complement(9033..9941)
                     /locus_tag="VFK00_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7593456.1"
                     /translation="MSQDLQKKLKSGWEGFTASEQRIAGYLLQNLSGIPFETATSLGQ
                     RVGVSAMTVGRFLRKLGYAGVAELKEELRGDTTWLKLYHSPAPAEGPGARDENLEGEI
                     RALSAIHALARGGEWKAIVRLLASADRVSVASFQLGRFLGLMFATLLQQIRPRVAFAS
                     GVDGAYTDVLIDSTENSCVVLIDERRYSRHFRILAEEVAARGIPLVILTDTQCYWARQ
                     LTPHVLMLPIQPGRAWHSFTAFGTLFNLLLGAVIRARGSDAVYERIERITAMRQQFIG
                     FSGPSLSSAAPAGEKPKRRARKRRPA"
     gene            complement(9981..>10425)
                     /locus_tag="VFK00_10695"
     CDS             complement(9981..>10425)
                     /locus_tag="VFK00_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7593457.1"
                     /translation="QLLPTLQQCRVSLVEQPFPVGEEAWLDGLDCPIRLAVDESVQDH
                     TDLAKVAGRVGTINIKLDKCGGLTEALSMASQAREMGFELMVGNMSGSSLAMAPAFVL
                     GQQCAVVDLDGPLYLHADYSPSARYENGMIWCPEELWGGALPAAA"
BASE COUNT         1675 a         3355 c         3459 g         1936 t
ORIGIN      
        1 ttgtgcagga gtgcgtccat ggatgccggc aaggtcggat cgttcatcag caaactgtgg
       61 gacgacgagg tgatcccgca gctcgtcgac tacatcagga tccccaacaa atcgccgatg
      121 tacgaccggg actgggtggc ccatgggtac atggatgccg cggtcgcgtt gatggaaaag
      181 tgggcgcgct cgaagctcga attgctgccg ggcgcgacgc tggaagtggt ccggctggag
      241 gggcgcacgc ccctgatctt catcgacgtg ccggcttcgg gcggggaagc ccgcgcggac
      301 gattgcgtgc tgctgtacgg gcatctcgac aaacagccgg aaatgaccgg ctggggcgaa
      361 gggctcgggc catggacccc ggtgctgaag ggcgaccgcc tgtacgggcg tggtggcgcc
      421 gacgatggct atgccatttt cggctcgctg tcggcgttgc tggccttgca cgagcagggc
      481 gtgccgcacg cccgttgcgt ggtgatgatc gaagcctgcg aggaatccgg cagctacgac
      541 ctgccgtact acgtggacca cctcgccccc cgaatcggcg agccgtcgct ggtggtgtgc
      601 ctcgattccg gatgcgcgaa ctacgaccag ttgtggctga ccacctcgct gcgcggcatg
      661 accggcggcg agttgacggt cgaggtgctg accgagggcg tgcattcggg cgacgcctcc
      721 ggggtcgtgc cggacagttt caggatcgtg cggcatctgc tgtcgcggct cgaggatccg
      781 gcgacgggca cgatcgtgcc gaagcaactg tacgtggagg tgccgccgga acgcgtggaa
      841 caggcgaagc agtgcgcgga catcctcggc gacgacgtct acgcgaagtt tccgtggatc
      901 gacagcatgc agcccgccag cgacgatcgg gtcgagctga tcctcaaccg gacctggcgt
      961 ccccagctcg ccatcaccgg tgccgacggg ctgccgtcgc tgcaaaacgc gggcaacgtc
     1021 ctgcggccgg gcaccgcgct gaagctgagc ctgcgcgtgc cgccgacgtt gaacggcagc
     1081 cgcgcgggcg acttcgtcaa gacattgctg gaatcggatc cgccttatgg tgcgaaggtc
     1141 accttcaagg tggagaagga tggcagtggc tggaatgcac cgcagctttc cgactggttg
     1201 agcagcgccg tttcgcaggc ctcggaggat gccttcggcg caccggcggc gtacatgggc
     1261 gaaggcggct cgatcccgtt catgggcatg ctgggcgaaa agttcccgaa ggcacagttc
     1321 cttatcaccg gcgtgctggg cccgcattcc aacgcacacg gccccaacga gttcctgcac
     1381 atcccgaccg gcaagaaggt gtcggcggtg gtggcgggcg tggtcgccgc gcatgcgctg
     1441 gcgagtgcgg ccggccagac ctgagctttc ccgcaggttg ccgccgtggc cagcatccgc
     1501 gcagcgaccg ccgcagacgc cggcgatctc gcggtactgt gcggacagct cggctatcct
     1561 gcggacccgg cgacggtcgc acggcgcctc gccgacattg ccgaatgtcg cagtggtgca
     1621 gtactggtcg cggaggccga tgacggtgcg gtggtgggtt gggcggaagc ctcgctgcaa
     1681 cggcacctcg tccacgatcc acgggtgatg ctggcgggat tggtcgtcgc ggagggggcg
     1741 cgcaaccagg gcgtaggtgt cgcgttgctg ggggcggtgg aagcctggtc gcgtgaacag
     1801 ggggcggccg agctgatcgt tcgatccaac atcgtccgtg agcgcgcgca ccgcttctat
     1861 ctccgcgaag gcttcgccga gaagaaacgc caggcgctat tcctcaagcc gctgtgacgc
     1921 cttcaaccaa gctacccttg cacgaccgca gaccggatcc tccccgatga tgtacgacct
     1981 gccacctgtc acccgcgccc tgctgatcgc caacgtggcg atcttcctgc tggagcagat
     2041 tccgtcgctg cagccgctgc tgcttgtcta cggagcgttg tggcctgtcg gcgcggcaca
     2101 tccgatggtg aatggatccg gacaggtcgt attggtcggc ttccacttct ggcagctcgt
     2161 gacgtacgcg ttcctgcacg gcggttgggg tcacatcttc ttcaacatgt tcgcgctgtg
     2221 gatgttcggc ggtcccatcg aacacctgct cggcgccagg cactacacgt tctactactt
     2281 tttctgcgcg gtgatcgcgg cggccgcgca tctcctggtc gcgcagttct tcacccatgg
     2341 cttctacccc acgctcggcg cctcgggcgc gattttcggt ctgctggtcg cgttcggcgt
     2401 gatgtacccg cggatgaaga tgttcctgct gttcttcccg gtgccgatgc cggcatgggt
     2461 gttcgtgctc ggttacatcg tgctggagct gttcttcggc gtcagcggct acgagtcggg
     2521 cgtcgcgcat ttcgcgcacc tcggcggcgc cgtgggaggc ttcgcgctgt tgatgtactg
     2581 gcgcggcaag ttgcccttca agcccaagcg aatcctgacg cgctgagcgt gcctgcatgt
     2641 gggcgctccg ccccatgttc gcgcgcatgg cgcgctcccg catcgctggt tgcgacggcg
     2701 ggtctgcatg gccgcgagca ccattcagcg cggctcgatc tgcagcgatc gcacgtacac
     2761 cacttcgcag gcgccggcac cggtatcggt gcgcgtcatc gaactgtgcc agcgccagcc
     2821 gcccggtttg tcggcctcca cgagaaagcc atcgagcgcc acgatgtcac ctgcgcgcac
     2881 gcgcctcagt gcgcgcgcga cgagtgcatt cgccgggatc atgtgcatgt tggcgctgtg
     2941 ggtttcgatt tcgcggcgcg gaatcggaaa tgactgcacc tgccagtaat agaagcgccc
     3001 gctttggctg atgtcgatgt cgcgcaacac cgcggtgtcg gacatggcac cccagcccac
     3061 ggcaaggtcg gtgggagaaa gcgccgcttc ggcgtcgagg cgatagtcct cccgcgagag
     3121 cacgcgtgcc gtcagcgaga atctcgcgag cggacgcagg gtcgcgtctt cgaacgggat
     3181 gggcggttgc ggtgcgtcga aatccacctg cacgggttcg tcgggcgcga gcacgcccgc
     3241 cgcgcgggcg acgggtcgat tgccccacca cgtccagccg gcccagaccg ccagcgcgag
     3301 cagcagccaa gccttccagt gccgcatcgg tgtcccccaa cgattccggc gcgaggatag
     3361 ctcagtcgaa gtcggcggtc agcgccttgc gttgcgcttc gtcgagggtg gcttccgcgc
     3421 ggtagtttgg atgttcgatg cgaaccgcca ccggcttgcc ggccttgaag tcggcgaccg
     3481 caccgacctc gagttcgaag cgcaggaagt gcacggccgc ggtcttttgc tcgtcgctgc
     3541 gcggcaggtc ctcgtcgggg atggcggtga ccggcggatg gcccccgacc acgagttgca
     3601 catcgtgctc cacgttgcgc aggcgcagca gttcgcgctt gcggacctcg acgtcgtcat
     3661 actcgatcag catcgtgcat ttgaggttcg cgccgtccgg gatcagcggg ttgtaggccg
     3721 cgagttcctc cgcgatgcct tccggttcga agatccgttc ggtccgaagc atctcctgga
     3781 cctggtactg gatcgtgagc cggtcttcga acagcagcgt gacgtgttcg ccgacccgga
     3841 ccttgcggtc gcgcttgtgg gcgagcaccc gcgtgcggaa gtccgcgcgc tcgcgcgcgt
     3901 attgttcgag ggacagcaag tcgtcttggg caagtggctt catgatttgg tgcttcggtt
     3961 tatgagtccc tggtcccggc tcccgagtcc cgcgcccgtg agatcaaatg ccgtaggcct
     4021 tccgcagcag cgacaccggg tgctgttgcg gcggctggtt ctgcagcccg tgcgcgaggt
     4081 gcgcgccggc catcgggcag tcgctggtgc tcatcacggc gcccgactcg gtgatccgcc
     4141 gttcgaccgg ccgggcgatc ttgcgcgaga gttcgtaggt ccgggtcttg acgccgtagg
     4201 tgccgtcgtg gccggagcag cgttcgatcg tcacgatttc ggtgtcgggt accagtgaca
     4261 ggatatcgcg ggtcttcggc ccgatcttct ggacccgctg gtggcaggga acgtgccagg
     4321 cgagctttcc gagcgatgcc ttgaaatcgg tcttcagcag gcctgccgca tggcgctccg
     4381 cgaggtattc gaacgggtcg aaaaacgcgc gctgcaccga gcgcacgtcc tcgtcgtccg
     4441 ggaacatcag cggaagttcc tgcttgaaca tcaggacgca cgacggaatc gccgcggtga
     4501 ggtcgaaacc gtcacgcacg gcatgtgcga gcaccgggat gttctggtcc ttgtatttct
     4561 tgacggaggc aagatcgccg agttccagct tgggcatccc gcagcagact tccttcgaga
     4621 ccagcttgac cgggatgtcg ttgtgcaaaa aaaccgcggc caggtcgtcc accaccgccg
     4681 gcaacacgct gtcgcagtag caggtcgcga agatcgcgat cttgccgcgg gtgcgcccgg
     4741 ccggcgtcgc gtcgcctgtg gcgtccagct tgcgaagctt cttgcgcgcg cgcgggtgct
     4801 tgtattcggg cacctcggcg tccgggtgga cgtgcagggt cttgtccagc agctggcgcg
     4861 ccggcttgct gcggttcaac gcgttgacgg tctcggcgac caccggacgt gcggtgatcc
     4921 ggccgaccgc ggtcgtgttc gacaggaatc gatcaccgag cgaggcgcca tcgcgctcga
     4981 actcgaacgc cttggcgcgc agcatcaggt gcgggaagtc gacgttccac ggatgcggcg
     5041 gcacgtacgg acacttggtc tggtagcaca ggtcgcacag gtagcactgc tcgaccacct
     5101 tgccgtaatc ggccttgtcg accccgtcga tttccatcgt ttccgattcg tcgatcaggt
     5161 cgaacagcgt gggaaaggcg tggcacaggc tgacgcagcg ccggcatccg tggcagatgt
     5221 cgaagatgcg ctccatctcc ttttcgagcg ccttctggtc ccggaatacc ggatcgtcct
     5281 ggcccagcgg atgacgcgtc ggggcttcga ggttgccttc acgcgtgccg gcggcgtcac
     5341 tgctcattcg ctctgtcctc tcgatgcacc cgcgggcgca tcgttgcccc gctgcgctcc
     5401 cgaaggttgg gttgactccc ggacgtggtc cgggaggaag cccaacggat gcgcgttgtc
     5461 gggcctcgct tgcgctcagc ccaaccttcg ttcatcgcag cggagccatg gcgtggcgcg
     5521 ttgatcggtt cgtcccaggc gggaggacat cacgttgggc ttcacccaga tgaagcttgg
     5581 gctcagccca acctacattc ctcaagcgga gcggtgattc cgatgcatgc ccgtgtccgc
     5641 ccgcgaagcg agcggagccg tgcgtcaccc gagctcgttc aacgccttgg tgaagcggtt
     5701 cgcgtgcgaa cgctcggcct tcgccagggt ttcgaaccag tcggcgattt cctcgaagcc
     5761 ttcgtcgcgc gcggacttgg ccatgccggg atacatgtcg gtgtactcgt gggtttcgcc
     5821 cgcgatcgcg ctcttgagat tggcatgggt ttcaccaaac gggaggcccg tggccgggtc
     5881 gcccacggcc tccaggtatt ccatgtgccc atgcgcgtgg cccgtctcgc cttcggcggt
     5941 ggagcggaac accgcagaca cgtcgttgta gccctcgacg tcggccttgg aggcgaaata
     6001 caggtaacgg cggttggcca tcgattcgcc ggcgaaagcg tccttgagat tctgttcggt
     6061 tttggagcct ttcaggtttg ccatgatgtc tacctcgggt ggggacggag cgttcgcggc
     6121 gcagtgccgg tcccttgaac ctagcgcacc cgccgcgcaa aatgaaattg attgatttga
     6181 tcgatgcgat agctatacgc tatcgcatgg gaatgctcac cggtgcagat cgcccgcggc
     6241 ttccggctgg tagtggatcg cggtcacctt caacttcagg gtgcgcccgt cgttgcccgg
     6301 ccagtcgatg ctctggccca cccgcaagcc cagcaacgcg ctcccgaccg gggccagcac
     6361 cgaaatcttg cccgcatcgg cgtcggcatc cttcggatac acaagcgtca acgtgtggtg
     6421 cctgttgctg gattcgtcca cgcaatcggc ggacgagttc atgctgacca cgtcgtgcgg
     6481 catcttcgcg ggctcgacga tttcggcgcg gtcgaattcc tcatgaagct ttttcgccac
     6541 cggcgaatcc cgcagcgcgg gcgcttccag caaggcttcg atgcgctcga catcgagcct
     6601 ggagagggtg atcggcgtcc gtgtatccat gtcaggcctt cgcgtggttt ttaccaccaa
     6661 catagcgagc cgcagtcgat ttcacaatgg gcggcgcggg cagggtggcg gggtcgagca
     6721 gccgtcgcgg caggttcttg aaaaccttcg acagctcgcg gaggaagccg tccatcgccg
     6781 agctccggcg ccagtacatc gcgatacgcc ggctaggcgc atggctgccg aacggagtga
     6841 ggtgcacgtt cgcggtgtgc gcgataggcg gttgcaccgc cagcaccggc agcagggtga
     6901 tcccgacatc cgccgcgacc atctgccgca gggtttcgag gctggtggcg cggaaaccct
     6961 gtcgctcgtc ggcgccggac atccggcata cctccagcgc ctggtcgcgt aggcaatggc
     7021 cgtcctcgag cagcaacagg cgctggccct cgagatcggc gagtttcaat ttgcgtcgcc
     7081 cggccagcgg gtgcgcctcg ggcgccgcca gcaggaacgg ttcctcgaac aggaattccg
     7141 cgtgcagggt ctcgtcgtgg acgggctgcg cgaggatcgc ggcatcgagc ttgccctcgc
     7201 gcaggcgctg gaccagttcc ggcgtctttt cctcgaccag caacaattcg agtcgcggga
     7261 accgctcgcg gatggtgccg agcacgtgcg gcagcaggta gggcccgagg gtcgggaaaa
     7321 tgcccaggcg cagcgtcccg gaagccggat cgtggtcgcg gcgcgccacg cgcttgatct
     7381 gctcgacctc gttcaacacc tcgcgtgcgc gcgcggcgat ctgcttgccg acctcggtca
     7441 gcagcacctt gcgggggctg cgctcgacca gggtgacgcc gagttcgtcc tcgagtttct
     7501 tgatctgggt cgagagggtc ggctggctca cgaagctggc ttcggcggcg cggccgaaat
     7561 gcttgtgatc ggcgagggcg acgagatatt ggaggtcgcg aaggttcatg gcgttgatgc
     7621 ggttgcgggc tcttgctgcg acatggcttt gtaatatacc aaagctatca tatgaattca
     7681 aataatcaat ttcatttatt gatcgcgatg ccgtatcttg gtcattgaac ggcggccgca
     7741 ggctgccggt gcaacctttc accacccacc aaggagcttc ccatgcttac cgttggaaac
     7801 aacttcccgc acttcaacct caaggccacc gtgtccatcg aaagcctgga caaggccttc
     7861 accgacatcg acaacagcac gtacaagggc aagtggacgt gcgtgttctt ctatccgaag
     7921 gacttcacct tcgtgtgccc caccgagatc aagggtttcg ccgatctcga cgcgcagttc
     7981 aaggatcgtg actgccagat cctcgccgcg agcaccgact ccgagttcgt gcacatggcg
     8041 tggcgcaagg accacaagga cctgcgtgac ctgccgttcc cgatgctggc ggacatcaac
     8101 aaggacctga cccgcgcgct cggcatcctc gacgaggagg gcgttgccaa gcgcgccgca
     8161 ttcctggtcg atcccgaagg cgtgatccgc ttcgtgtacg tcacggaagg ttcggtcggc
     8221 cgcaaccccg ccgaagtgct gcgcgtcctg gacgcgctgc agaccgacga gctgtgcccg
     8281 tgcaactgga acaagggcga ggaaaccctg aaggcagcgt gacttcgacc gtcgcgtggc
     8341 gcgctcggtg tctcttgtag gagcgcgctt tgcgcgcgac cgctgcaacg ccggagcgcc
     8401 cttggtcgcg gccaagggcc gctcctactg acggtcgatt ccgtctcgaa cgaaggagaa
     8461 cgtcatgaat cttgatcagt tgaaatccca attgcccgca tacgccaagg accttcgcct
     8521 caacctcgaa tcggtgttgg gcgaaggcgg cgcgccgggc ctcggccaga agcagatcgc
     8581 gatggtcgcg ctggcttcgg cgatcgcatc gcgccacgcc ccgctgaccg aagcgatcac
     8641 gcaattcgcc tcgcagcatg ccggcgagaa ggatctcgag ggcgcgcgca ccgccgccgc
     8701 gctgatgggc atgaccaaca tctactaccg cttcctgcac ctcgtcgaga acgacgagta
     8761 cggcacgttg cgcgccggcc tgcgcatgaa cgccatggcg agccccggct gcgacaaggt
     8821 cgatttcgat ctcgcctcgg tcgcggtgtc cgccatcaac ggctgcggca gttgcgtggc
     8881 ctcgcacgag cgcacccttc gcaagcacga cgtttccgcg caggccgtgc aaagtgcggc
     8941 gcggatcgcg gccgtgatcc atgcagtcgc cgtggtgctc gagcagcagg aagccgcggc
     9001 caccgcttcg gccgccgagg cggcgtgagc cgctaggctg ggcggcgctt gcgggcgcga
     9061 cgcttcggtt tctcgcccgc gggcgcagcg gacgacaacg acggcccgct gaagccgatg
     9121 aattgctgcc gcatcgcggt gatccgttcg atgcgttcgt acaccgcgtc gctgccgcgc
     9181 gctcggatca ccgcgccgag cagcaggttg aacaaggtgc cgaacgcggt gaagctgtgc
     9241 cacgcacgtc ccggctggat cggcagcatc aggacgtgcg gcgtcaactg gcgcgcccag
     9301 tagcattggg tgtcggtgag gatcaccagc gggatcccgc gcgccgccac ctcttcggcc
     9361 aggatccgga aatgccgcga atagcggcgt tcgtcgatca gcaccacgca actgttttcg
     9421 gtcgagtcga tcagcacgtc ggtgtaggcg ccgtccacgc ccgacgcgaa cgcgacccgc
     9481 ggacgtatct gctgcagcaa ggtcgcgaac atgagcccga ggaagcgccc gagctggaaa
     9541 ctcgccaccg ataccctgtc cgccgatgcc agcaggcgca cgatggcctt ccattccccg
     9601 ccgcgcgcca atgcatggat cgcggatagc gcccggattt ctccttcgag gttttcatcg
     9661 cgcgcacccg gcccttccgc gggcgccggg ctgtggtaca gcttcaacca ggtcgtgtcg
     9721 ccgcgcaatt cctccttcag ttcggcgacg cccgcgtagc cgagcttgcg caggaaccga
     9781 cccaccgtca tcgcgctgac accgacgcgc tgccccagcg aggtcgcggt ttcgaacggg
     9841 atgccgctca ggttttgcag caggtagccg gcaatgcgct gttcggaggc ggtgaagccc
     9901 tcccagccgc tcttgagttt tttctggaga tcctggctca tcgtcgtgcg gtcatcgggc
     9961 cacgtcgcgc gttcgcatcc tcatgcggcc gcaggcaacg cgccgcccca aagctcttcc
    10021 ggacaccaga tcatcccgtt ttcgtatcgc gccgagggcg aatagtcggc gtgcaggtac
    10081 agggggccgt ccaggtccac caccgcgcat tgctggccca gcacgaacgc aggcgccatc
    10141 gccagcgacg aaccggacat gttgccgacc atcagctcga agcccatctc gcgggcctgg
    10201 ctggccatgg acaaggcctc ggtgaggccg ccgcacttgt ccagcttgat gttgatggta
    10261 ccgacgcgcc cggcgacctt cgcgagatcg gtgtggtcct gcacgctttc gtccacggca
    10321 aggcggatcg ggcaatccag gccgtccagc caggcctcct cgccgaccgg aaacggttgc
    10381 tctaccagcg acacccggca ctgctgcagt gtcggcagca actgc
//
LOCUS       DASSNH010000290         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1789017, whole genome shotgun sequence.
ACCESSION   DASSNH010000290 DASSNH010000000
VERSION     DASSNH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789017"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /gene="gcvPA"
                     /locus_tag="VFK00_10700"
     CDS             complement(<1..1600)
                     /gene="gcvPA"
                     /locus_tag="VFK00_10700"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7593458.1"
                     /translation="MTQNTPSLHGLEQHDAFIGRHIGPDDTEIATMLEAVGYDSLDAL
                     VDAIVPAAIRQKNPLALPPAVTENEALRKIRAIADRNEVFKSFIGQGYYGTHVPNVIL
                     RNVLENPAWYTAYTPYQAEISQGRMEALLNFQTMCADLTGMEIANASLLDEATAAAEA
                     MTLAKRSSKSKSNVFFVSKNVHPQTIEVLKTRAEPLGIELRIDDEANAANVDCYGALL
                     QYPDTFGTIRDYKALSDALHAKGALAAVATDLLALTLIAPPGEWGADIVVGNSQRFGV
                     PFGFGGPHAAFMACRDAFKRSMPGRLIGVSKDAENNNAYRLTLQTREQHIRREKATSN
                     ICTAQVLLAVMAAMYAVYHGPDGLVRIARRTHRLATILAAALRKTGIAVGGDFFDTLH
                     VTGIDAHTIHTMANAAHINLRRIDATSLAISLDETTTREDVVQLASLFGARIDDIDEL
                     DRETPDALPESLTRGSEFLSHPVFNSHHSEHEMLRYLRQLADKDLALDRSMIPLGSCT
                     MKLNATAEMIPITWPEFAQIHPLAP"
     regulatory      complement(1819..1924)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2069..2509
                     /locus_tag="VFK00_10705"
     CDS             2069..2509
                     /locus_tag="VFK00_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7593459.1"
                     /translation="MSPPPFRLHQLDHVVLRVRDVERMRRFYCDVLGCTNELDQPDIG
                     LFQLRAGSSLIDLVDVEGELGRSGGPAPAADGHNMDHVCLSVDGYDEAEIVAHLESQG
                     VVVGEIGLRYGAEGQGPSIYLFDPEGNKIELKGPPIRGSRAADT"
     gene            2534..2809
                     /locus_tag="VFK00_10710"
     CDS             2534..2809
                     /locus_tag="VFK00_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593460.1"
                     /translation="MPCLMVLFALFVPRVVIVLLWLFTSWFSGIFSMALWPVLGFIFL
                     PTTLLWYTAVQHWWGGHWSLWPIVGIVIALLIDLSPASGRRRYVRRL"
     gene            3114..>3964
                     /locus_tag="VFK00_10715"
     CDS             3114..>3964
                     /locus_tag="VFK00_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET7593461.1"
                     /translation="MLLVSAMLAFGAGVHAGQLTAADYARAVRFLSQETAPLVDHDVQ
                     RVTWLDDAHFWYRDHDASGDHFMEMDATTGKAAPAFDQAKLATALGKARDKPVDAKKF
                     PKHFDFRRVPGGNLDVQLGDDWYRCDLSGVGVCTALDKLLQTGDEPGALSPDRKLLAF
                     VRDWNLWVRDLATGKETQLTADGVKDYGYATQNAGWVHGDGAILRWSPDSTKIATYRQ
                     DQRKVGEMYTVSTRVGHPELDAWPYPLPGDEHVFMIEPVVIDISSKKMVRLQMAPEQR
                     LSTLCDTL"
BASE COUNT          606 a         1223 c         1372 g          763 t
ORIGIN      
        1 gcggcgccag cggatggatc tgcgcgaatt cgggccaggt gatcgggatc atctcggcgg
       61 tcgcgttcaa cttcatggtg cacgagccga gcgggatcat gctgcggtcg agcgcgaggt
      121 ccttgtcggc gagctggcgc aagtaacgca gcatctcgtg ttcggagtgg tgcgaattga
      181 ataccgggtg gctgagaaac tccgaaccgc gtgtcagtga ctcgggcagc gcgtcaggcg
      241 tctcgcgatc gagttcgtcg atgtcgtcga tgcgcgcgcc gaacaacgat gcgagttgca
      301 ccacgtcttc gcgggtggtg gtttcgtcca ggctgatcgc cagcgaagtc gcgtcgatgc
      361 ggcgcaggtt gatgtgtgcc gcgttggcca tcgtatggat cgtgtgcgcg tcgatgccgg
      421 tgacgtgcag ggtgtcgaag aagtcgccgc cgactgcgat gccggtcttg cgcagggcgg
      481 ctgcgaggat tgtggcgagc cggtgggtgc gccgggcgat gcgaacgagt ccgtccgggc
      541 cgtggtatac cgcatacatc gcggccatca ccgccagcag cacctgcgcg gtgcagatgt
      601 tggaggtcgc cttctcgcgg cgaatgtgtt gctcgcgggt ctggagcgtg aggcggtagg
      661 cattgttgtt ttcggcgtcc ttggacaccc cgatcaggcg gcccggcatc gagcgcttga
      721 aggcgtcgcg gcaggccatg aacgcggcgt gcgggccgcc gaaaccgaac ggcacgccga
      781 agcgctgcga gttgccgacc acgatgtcgg cgccccattc gccgggcggc gcgatcagcg
      841 tcagcgcgag caggtcggtg gcgaccgcgg ccagcgcgcc cttggcgtgc agcgcgtcgc
      901 tcagcgcctt gtaatcgcgg atcgtgccga aggtgtcggg gtattgcagc agtgcaccgt
      961 agcagtcgac attggcagca ttcgcctcgt catcgatgcg gagttcgatg ccgagcggct
     1021 cggcgcgggt tttcagcacc tcgatggttt gcgggtgcac gttcttcgag acgaagaaca
     1081 cgttcgattt cgatttggac gagcgcttcg ccagcgtcat cgcctcggcc gccgcggtgg
     1141 cctcgtccaa aagtgacgcg ttcgcgatct ccatgccagt gagatctgcg cacatcgtct
     1201 ggaaattgag cagcgcctcc atgcgaccct gcgaaatctc ggcctggtac ggcgtgtagg
     1261 ccgtgtacca cgccggattc tcgaggacgt tgcgcaggat gacgttgggc acgtgggtgc
     1321 cgtaatagcc ctggccgatg aagctcttga agacttcgtt cctgtccgcg atcgcgcgga
     1381 tcttccggag cgcttcgttc tcggtaacgg ccggcggcaa cgccagcggg ttcttctggc
     1441 ggatcgcggc ggggacgatc gcatcgacca acgcgtcgag cgagtcgtag ccgacggctt
     1501 cgagcatcgt cgcgatttcg gtgtcgtcgg gaccgatgtg gcggccgatg aacgcgtcgt
     1561 gctgctcgag gccgtgcagg gaaggggtgt tctgggtcat ggcgggcgtc cgggagcggt
     1621 ggcgtcggga acaactgcgt cgtcatcccg gccatggacc ggccgggacc cagtgccatg
     1681 gaaggaaagc actggattac aaagtccgtc catggacttt gccctttcgg gccggccttc
     1741 ggccgttcaa attcgtttca gacgaatttg tccgggcctg cgccggaatg acgacttgaa
     1801 aacaccaggg tgtcttcgtc gcgcccctct gtccttctgc ctgagagttt cgaagctcgc
     1861 gctccttgcc ccttcggcgc cggtcgccgc tgctgaacgg tccggtctct ccagagtttt
     1921 tgatcgtggt ggtatcgagc ctgagcgatt acgggcgttg cgccttcggc agcggcggtg
     1981 ccgcttcttc caccactcgg caattataca ggttggcggg attgcgatcg attaggatgg
     2041 ctgctcgccc ggaattcccg tgcctgccat gtccccgcca ccatttcgcc tgcaccaact
     2101 cgatcacgtg gtcctgcggg tccgcgacgt ggaacggatg cggcgcttct attgcgacgt
     2161 gctcggttgc acgaatgaac tggaccagcc ggacatcggc ttgttccagt tgcgggccgg
     2221 gtcgtcgctg atcgacctgg tggacgtcga aggcgagctg ggcaggagcg gcggtccggc
     2281 gcccgccgcc gacgggcaca acatggacca cgtctgcctc agcgtcgacg gctacgacga
     2341 agccgagatc gtcgcgcacc tggagtcgca aggggtggtc gtcggcgaga tcggcctgcg
     2401 ctacggcgcg gaagggcagg ggccgtcgat ctacctgttc gacccggaag gcaacaagat
     2461 cgaattgaag ggcccgccga tccgtggatc gcgcgcggcc gacacctgac cccgtcgcca
     2521 cgaggagact gccatgccgt gcctgatggt cctgttcgcg ttgttcgtgc cacgcgtggt
     2581 gatcgtgctg ctgtggctgt tcacgagctg gttcagcggc attttctcga tggccttgtg
     2641 gcccgtgctg ggcttcatct tcctgccgac cacgctgctc tggtacaccg ccgtgcagca
     2701 ctggtggggc ggccactggt cgctgtggcc gatcgtcgga atcgtgatcg cgctgctgat
     2761 cgacctgtcg cccgccagcg ggcgccgcag gtacgtgcgg cggctgtgac ccagggctgc
     2821 ggtcgacagc ctgtttcacg gcaggcggcg cacggtctcc gccctgccgg aagtgcaggg
     2881 aggcagcggt agcttttttt ggcaagcagg catccctgcg gcggaatccg gcccgcactt
     2941 ccctgtgcgc ggccgcttgc cggcgcggca tgccccccgg gcatctcgcg aaagccggct
     3001 cgccctacga cttctggcag gggtcgcacg cttgcgcgcc gtctaggatc agtgggttgc
     3061 gtttcgttcg gccgaccgga gtcgccatgc cttttcttcg ttgcgttgct tgcgtgttgc
     3121 tcgtttcggc catgctcgcc ttcggtgccg gcgtccatgc aggccagttg actgccgcgg
     3181 attatgcgcg tgcggtcagg ttcctgtcgc aggagaccgc gccgctggtc gaccacgacg
     3241 tgcagcgggt gacatggctg gacgacgcgc atttctggta ccgcgaccac gatgcgtcgg
     3301 gcgaccactt catggaaatg gatgccacga ccggcaaggc cgcgcccgcc ttcgaccagg
     3361 cgaagctcgc gactgcgctg ggcaaggcgc gcgacaagcc cgtcgatgcg aagaagtttc
     3421 ccaagcactt cgatttccgc cgcgtgcccg gcggcaacct ggatgtccaa ctgggtgacg
     3481 actggtaccg ctgcgatctt tccggggtag gtgtctgcac cgcgctcgac aaactgctgc
     3541 agacgggcga cgaacccggc gccctctctc ccgacaggaa gctgctggcg ttcgtgcgcg
     3601 actggaatct gtgggtgcgc gacctcgcga ccggcaagga aacccagctc accgcggacg
     3661 gcgtgaagga ttacggctac gcgacccaga acgccggctg ggtgcacggc gacggcgcga
     3721 tcctgcgctg gtcgcccgat tcgacgaaga tcgcaaccta ccggcaggac cagcgcaagg
     3781 ttggcgagat gtacaccgtc tccaccaggg tcgggcatcc ggaactcgac gcgtggccgt
     3841 acccgctgcc gggcgacgag cacgtcttca tgatcgagcc ggtggtgatc gacatctcgt
     3901 cgaagaagat ggtgcgcctg cagatggcgc cggaacagcg gctctccacg ctgtgcgaca
     3961 cgct
//
LOCUS       DASSNH010000291        11261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_548235, whole genome shotgun sequence.
ACCESSION   DASSNH010000291 DASSNH010000000
VERSION     DASSNH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548235"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFK00_10720"
     CDS             <1..827
                     /locus_tag="VFK00_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593462.1"
                     /translation="GDWQPVSDFGFGDITNLSLLECTSWGDHLYASTGNNKGFQLWRS
                     TCEGEPPYHWEQVLRDGCGRGSLNQGGTSLYPFRDALYIGTGIQRGGNDKENKIGPAG
                     SELIRLNKDLSWDLIVGDPKDTPDGRKEPKSGRYAGFDQFFNGYFWRMAEHDGWLYLG
                     TFEWSSVLGYISRSRWPRVMSNLIARVGPQKILEYGSGFDLYRSRDGDNWVPVTTNGM
                     DNPYNMGMRTICSTPYGLFLGTANPWGPRIMPLDGRNRFVHNPRGGCEVFFARNLA"
     gene            907..1854
                     /locus_tag="VFK00_10725"
     CDS             907..1854
                     /locus_tag="VFK00_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7593463.1"
                     /translation="MMLLSSIGKKALTALLFGALTSTSAHAMQATQRPRMGGQEGNRG
                     LLQRLMRRGNPQPLTLPSGARLLKDVAYGSDERQKMDVYIPQGAHDAPVILMVHGGGW
                     RRGNKELPGVVQNKIDYFLPKGYIFISTNYRLVPEVTPVTEAEDVAHALAYGQQHARE
                     WGADPSRFVLMGHSAGAHLVVLVSSAPSIWQGAGAKAWLGTVGLDSGAYNVAEIMNQR
                     HFGLYDIAFGDDPQLWQEASPTLRLHAAPPPMLLVCDAERLTSCAAARAYADKAQSLG
                     GRVTVFPVSAGHGEINASVGTESPLTSRIDQFLQSVGLH"
     gene            complement(1934..2527)
                     /locus_tag="VFK00_10730"
     CDS             complement(1934..2527)
                     /locus_tag="VFK00_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593464.1"
                     /translation="MANRHSYQTITDWQRPDEATGEAIRAFWIREQANVAGSEATRRL
                     AEVVAHIVDENGEIAAVATVTPKILPRLGQPLYYYRCFVGKAWRTERLLRPLMRHTQK
                     VLEAYARENDYPCIGMLLELENQGFADTLRRAHWTGLGFSYIGVSPRGLDLRVWYFRG
                     ARLKTPAQLASLRTANAGTMQLRAAAAAARQKQGASS"
     gene            2583..2669
                     /locus_tag="VFK00_10735"
     tRNA            2583..2669
                     /locus_tag="VFK00_10735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2617..2619,aa:Leu,seq:taa)
     gene            complement(2754..3281)
                     /locus_tag="VFK00_10740"
     CDS             complement(2754..3281)
                     /locus_tag="VFK00_10740"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HET7593465.1"
                     /translation="MSLAPAFNLQAWIDEHRHLLKPPVGNKCIVDGDFIIMIVGGPNA
                     RSDYHWDEGPEFFHQLEGEMVLRVQDEGVRRDIPIRAGEVFYLPPRTPHSPQRMPDSI
                     GLVIERRRLPHEKDGLLWFCENCNRKLYEEYFVLGSIEKDFPPVFERYYGSKEARTCS
                     NCGHVDQPPEGHAAG"
     gene            3400..4533
                     /locus_tag="VFK00_10745"
     CDS             3400..4533
                     /locus_tag="VFK00_10745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7593466.1"
                     /translation="MLHIRLRPLWLLLCLLALNACAGAPTKVAQTFAPRTVTILGSGD
                     VLIHPPLVEQAREDARAKGEAGYDFHAILAAIAPDVRGADLAICELETPLAPAAGPFS
                     GWPEFDAPPQVLTALKGVGYDSCTTASNHSLDKGFAGIVRTLDELDAAGLEHTGSARS
                     EREAETPLVVTLPNGVEVAQLAYSYGFNAHQPPADKLWSVNPIDVKAILAAAQRAKRA
                     GADIVVLSMHWGTEYDHLATPTQLAQAKRLLASPDIDLILGDHAHVVQPMQKIGDKWV
                     IYCMGNLVARHEKPIDDSREGILAKFTFTEVRPHVFRVSRAEAIPVWMQIEPTLRLID
                     LPRALGDPATTAEQRAAYQSAWNRIAGYLDAYGAVKDGLVIER"
     gene            complement(4788..6197)
                     /locus_tag="VFK00_10750"
     CDS             complement(4788..6197)
                     /locus_tag="VFK00_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593467.1"
                     /translation="MSEISLMESRKLRICWPLALAAAFLASPAFAARIDCSRAPDPAI
                     RTICAHPNILSLDDRVSSAYSAALARNPSRADDLRQDEINWLGERNREIGWLPGDDLD
                     SAPLSGTAEATLAHFYQLRIAFLRNIDNPAATQDAPLARALLESVAALSPGATDPLEA
                     LRSAGIVVVADERNASDAEHTIDTLAAPPDAALHAELAPFRSYPYTVEYLPSAGLGGA
                     FTVQGTGDCQSWVLFEKRADATVPVRGSGSLGGCTRDQGATGYLALIRGHPAVLTVTD
                     DPAFRNVTDLQWQEWLGGDKWGPETRIRLRYGYSLEPDRQMYCKSPSAQCTSSMSAAL
                     NGPAHCPDDFHQCALITTVALGAAKQYLRNALGLAARSGGPASEQARFTALLRLAPGR
                     KDWASCANPVWFPVHLGGKLALGGITESHLGCHPGGGALDVGVWATTGRGARQWWFVD
                     ETIKVARQRLLAASVVPPA"
     gene            complement(6415..7056)
                     /locus_tag="VFK00_10755"
     CDS             complement(6415..7056)
                     /locus_tag="VFK00_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593468.1"
                     /translation="MVLPDRHLDLGCGSRPRNPYQRGELCGVDILTFAEAPGFDYRAA
                     NLAVGPIPFDDDHFGSVSAFDFIEHVPRILPAAGPAGTCFPFIRLMNEIWRVLAPGGR
                     FYALTPAYPHAEAFTDPTHVNVITAHTHEYFCGDEPPARVYGFVGRFRALDVRWTLRG
                     DAYSAIRGSAENYSGTKRLARKLRAVSRTLRGKQATAKPPYLLWELEAEKPSL"
     gene            complement(7056..8951)
                     /gene="parE"
                     /locus_tag="VFK00_10760"
     CDS             complement(7056..8951)
                     /gene="parE"
                     /locus_tag="VFK00_10760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET7593469.1"
                     /translation="MAPSTRYNSADIEVLSGLEPVRRRPGMYTDTTRPNHLVQEVVDN
                     SVDEALTGYAKEIDVTVHKDGSVTVSDDGRGMPVDIHPEEGLPGVELILTRLHAGAKF
                     SNRNYTFSGGLHGVGVSVVNALSSFLEVLIRRDGTEYRMRFKDGEAIGKLETLGEVAK
                     RRTGTVLRFLPDPKYFDSPKIALPKLKHLLRAKAVLCAGLTVRLADEASGDREEWRYE
                     DGLAEYLTSELDGATCLPPELFIHHAQREDGGLDAAFAWVPEGELVQESYVNLIPTIQ
                     GGTHVNGLRSGLTNALREFCDLRNLLPRGVKLSPEDVWERVAYVLSVKMRDPQFAGQT
                     KERLSSRDAAGMTEGVVHDAFSLWLNQNVAAGEAIAQLAIEHAAARMKAAKQVVRKKV
                     TQGPALPGKLADCASSDPDRTELFLVEGDSAGGSAKQARDKDYQAILPLRGKILNTWE
                     VESGAVLASEEVHNLAVAIGCDPGKDDLSGLRYGKVIILADADSDGLHIATLLSALFL
                     KHFPALVREGHVCVAMPPLFRVDVGKQVFYCLDEGERDALLKRIEREKIKGAVSVTRF
                     KGLGEMNPAQLRESTIHPDTRRLVQLTVDDDTATSQLMDMLLAKKRASDRRNWLEEKG
                     DLASLEV"
     gene            9447..9716
                     /gene="rpsO"
                     /locus_tag="VFK00_10765"
     CDS             9447..9716
                     /gene="rpsO"
                     /locus_tag="VFK00_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7593470.1"
                     /translation="MPLSTEQTSKIIEDYRRAPTDTGSPEVQIALLSARIQQLSGHFE
                     SHKKDHHSRRGLLKMVNHRRRLLGYLKSKDAERYQALIGRLGLRR"
     gene            9823..>11261
                     /gene="pnp"
                     /locus_tag="VFK00_10770"
     CDS             9823..>11261
                     /gene="pnp"
                     /locus_tag="VFK00_10770"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7593471.1"
                     /translation="MAKVTKTFQYGQHPVTIETGEIARQASGAVVVSMAGTVVLVAAV
                     AAPKAREGQDFFPLTVDYQEKFYSAGRIPGGFFKREGRPTEKETLTSRLIDRPIRPLF
                     PEAFKNEVQVVATVISLNPEVNGDIPALLGASAALTLAGIPFKGPIGAARVGYVDGKY
                     VLNPTATELQSSDLDLVVAGTANAVLMVESEAKLLSEDVMLGAVTFGHKEMQAAVRAI
                     AELATEVGKPSWNWQAPERDAQLVAAVRGEVGDKLAAAFQVKDKLQRRDAIAALKADV
                     KEALKTQAEEKGWPVADIVGEFEDLEYHTVRDSVLKTRQRIDGRGLEDIRPINVRVGV
                     LPRTHGSALFTRGETQAIVTVTLGTGRDSQIIDAPEGESKDAFLFHYNFPPYSVGETG
                     RMGGPKRREIGHGRLARRGVQAVKPTMEEFPYVIRVVSEITESNGSSSMASVCGSSLA
                     MMDAGVPLKAPVAGIAMGLVKEGEDFVVLS"
BASE COUNT         1952 a         3689 c         3672 g         1948 t
ORIGIN      
        1 cgggtgactg gcagccggtc agcgatttcg gcttcggcga catcaccaat ctctcgctgc
       61 tcgagtgcac gtcctggggc gatcacctct atgcgagcac cggcaacaac aagggattcc
      121 agttgtggcg cagcacctgc gagggcgaac cgccatacca ctgggagcag gtactgcgcg
      181 atggctgcgg gcggggcagc ctgaaccagg gcggtaccag cctctacccg ttccgggacg
      241 ccttgtacat cggcaccggc atccagcgcg gcggcaatga caaggaaaac aagatcggcc
      301 ccgccggctc ggaactgatt cgcctgaaca aggacctgtc gtgggacttg atcgtcggcg
      361 atccgaaaga cacgccggat ggccgcaagg aaccgaagtc ggggcgctac gccggcttcg
      421 accagttctt caacggctat ttctggcgca tggccgaaca cgatggctgg ctctatctcg
      481 ggaccttcga gtggagcagc gtgctcggtt acatcagtcg cagccgctgg ccacgtgtca
      541 tgtccaacct gatcgcgcgg gtcggcccgc agaagatcct cgagtacgga tcgggattcg
      601 acctctaccg cagccgcgac ggggacaact gggttccggt caccaccaac ggaatggaca
      661 acccctacaa catgggcatg cgcacgatct gctccacgcc ttacggcctg ttcctcggca
      721 cggccaatcc atggggcccg cggatcatgc cgctggacgg tcgcaaccgc ttcgtgcaca
      781 atccgcgcgg cggctgcgaa gtgttcttcg cgaggaacct ggcgtgactt cgccccgagc
      841 cggctccctt tccgtggcct gcaaggtgga tgatcctgca gcgccgccgg cggatcggag
      901 aagcgtatga tgctgctgtc ctcgattggc aagaaggcgc tgacggcgct gctgttcggt
      961 gccctcactt cgacttcggc acacgctatg caggctaccc agagaccgcg gatgggcggg
     1021 caggagggga atcgcggcct cctgcaacgg ctgatgcggc ggggcaatcc ccaaccgttg
     1081 acgttgccgt cgggtgcccg gctgctcaag gatgttgcct acggctccga cgagcggcag
     1141 aaaatggacg tgtacatccc gcagggtgcg cacgacgccc ccgtgatcct gatggtgcac
     1201 ggcggtggct ggaggcgcgg caacaaggaa ctccccggcg tcgtccagaa caagatcgac
     1261 tacttcctgc cgaagggcta catcttcatt tcgaccaact accggctcgt gcccgaagtg
     1321 accccggtga ccgaggcgga ggatgtcgcc cacgcgcttg cctatggcca gcagcatgcg
     1381 cgcgagtggg gcgctgatcc ctccaggttc gtgttgatgg ggcattccgc cggcgcgcac
     1441 ctggtcgtgc tggtgagttc ggcgccgtcg atctggcaag gagcgggggc caaggcctgg
     1501 ctcggcacgg tgggcctcga cagcggcgcg tacaacgtcg ccgaaatcat gaaccagagg
     1561 cacttcggcc tctacgacat cgcgttcggg gacgacccgc aactgtggca ggaagcctca
     1621 ccgaccttgc gcctgcacgc ggcgccacca ccgatgttgc tggtatgcga tgcggaacgc
     1681 ctcaccagtt gcgccgccgc ccgtgcgtac gcggacaagg cgcagtccct gggcggtcgc
     1741 gtgacggtgt tccccgtctc cgcaggacac ggcgagatca acgccagcgt cgggacggag
     1801 agtccactca ccagccgcat cgaccagttc ctgcagtcgg tcggactgca ttgatccggt
     1861 gccggcggtc ctgccataac cttcatcggc cgatagttgc ccgctcgagg cagcggccca
     1921 tcctgcaccg tgcctagctg gatgcccctt gcttttgccg cgcagctgca gcggccgcgc
     1981 gcagttgcat ggttccggcg tttgccgttc gcagggaagc gagctgtgcg ggtgtcttga
     2041 gtcgcgctcc acggaaatac cagacgcgca aatccaggcc acgcgggctg acgccgatgt
     2101 aggaaaagcc gagaccggtc cagtgcgccc gtcgcagcgt gtcggcgaaa ccctggttct
     2161 ccagttccag cagcatgccg atgcacggat aatcattttc gcgggcgtac gcttccagca
     2221 ctttctgggt atgccgcatc aacggccgca gcagccgttc ggtacgccac gccttgccca
     2281 cgaagcagcg gtagtaatag agcggctgac ccagccgcgg caggatcttc ggtgtcacgg
     2341 tcgccaccgc cgcgatttcg ccgttctcgt ccacgatgtg cgccacgact tccgccaggc
     2401 gccgcgtcgc ttcgctgccc gcgacattgg cttgttcgcg aatccagaag gcgcggatcg
     2461 cctcgccggt tgcctcatcg ggcctttgcc agtcggtgat ggtctggtac gaatgccgat
     2521 tcgccatgtg ggtcggtcct ccccaatcca cggctactat agcgactcgc gacgcaggtc
     2581 acgcgcgggt ggcgaaaccg gtagacgcag gggacttaaa atcccccggc ctcgcggcca
     2641 tgtgggttcg acccccaccc cgcgcaccac ggcattccag gcatcgcagg gtgaacccgc
     2701 gaagcggttc ccaccgcaca ttgcccttgg tggaagccct tgccgcttgc accctacccg
     2761 gcagcgtgcc cttcgggtgg ctggtcgacg tggccgcagt tgctgcaggt ccgtgcttcc
     2821 ttcgaaccgt agtagcgttc gaacacgggc ggaaaatcct tttcgatgct cccgaggaca
     2881 aaatactcct cgtacagctt gcggttgcag ttttcgcaga accacagcag cccgtccttt
     2941 tcgtgcggca ggcggcggcg ttcgatcacc aggccgatcg aatccggcat ccgctgcggc
     3001 gaatgcggcg tgcgcggcgg caggtagaaa acctcgccgg cgcggatcgg gatgtcgcgc
     3061 cgcacaccct cgtcctgtac gcgcagcacc atttcgcctt cgagctggtg gaagaattcc
     3121 gggccttcgt cccagtggta gtcgctgcgc gcgttcgggc cgccgacgat catgatgatg
     3181 aagtcgccat cgacgatgca cttgttgccc accggcggct tgagcaggtg ccggtgttcg
     3241 tcgatccagg cttgcaggtt gaaggcgggc gcgagggaca tgctggcggt cggaagcgac
     3301 gggtccgcat accatagcgc ttgcgtgccc gcggcggaac gattgacttg acgcgcgccc
     3361 gcggccacgc tttgcccggt cgccccgggg accaaccgca tgttgcacat ccggctccgc
     3421 ccgttatggc tgcttctgtg cctgctcgcg ctgaatgcct gcgcgggcgc accgacgaaa
     3481 gtcgcgcaaa cgttcgctcc gcggacggtg accatcctcg gttccggcga cgtgctgatc
     3541 cacccgccgc tggtcgagca ggcccgcgag gatgcgcggg cgaaaggcga ggcgggctat
     3601 gacttccatg cgatcctcgc tgcgatcgca ccggacgtcc gcggcgcgga tctcgcgatc
     3661 tgcgaactcg aaacgccctt ggcgcccgcc gccggaccgt tctccggctg gccggaattc
     3721 gacgccccgc cgcaggtgct caccgcgctg aagggcgtcg gttacgacag ttgcaccacc
     3781 gcgtccaacc actcgctcga caagggtttc gcggggatcg tgcgcaccct cgacgaactc
     3841 gatgcagctg gcctcgagca caccggcagt gcgcgcagcg aaagggaggc cgaaacgcca
     3901 ctggtggtca cgctgccgaa cggcgtggag gtcgcgcagc tcgcatattc gtacggcttc
     3961 aacgcccacc agccgccggc ggacaagctg tggtcggtca atccgatcga cgtcaaggcg
     4021 atcctcgccg ctgcccaacg cgccaagcgg gcaggcgcgg acatcgtggt gctgtcgatg
     4081 cactggggca ccgaatacga ccacctcgcg acgccgaccc agcttgccca ggccaagcgc
     4141 ctgctcgcgt cgcccgacat cgacctgatc ctcggcgatc acgcgcacgt ggtccagccg
     4201 atgcagaaga tcggcgacaa gtgggtgatc tactgcatgg gcaacctggt cgcccgccac
     4261 gaaaagccga tcgacgacag ccgtgaaggc atcctcgcga aattcacctt cacggaggtg
     4321 cgcccgcacg tattccgcgt cagccgcgcc gaagcgattc cggtgtggat gcagatcgag
     4381 ccgaccttgc gcctcatcga cttgccgcgc gcgctggggg atcccgcgac gacggccgaa
     4441 caacgggccg cgtaccagag cgcgtggaac cgcatcgcgg gctatctcga cgcctacggc
     4501 gcggtgaaag atggtctcgt catcgagcgg tgaccgtctg gaaccggaag gcttcgcctt
     4561 tttcccttag aggattccga cggccctggc ttgtgagtgg tgtgctgact ccggacagcc
     4621 ggagtttgca cggcgcaggt agcggccagc ggggacagcc gccgccaatc ttcttctctg
     4681 cctgcttgac gggagcgctt gcggatcatg ctggtgcatt ccctgcgacc gtggtggggt
     4741 gtgtcggcgc tgtgctcgac ccatccgcgc cgcatggcca ggaaacctca ggcgggtggg
     4801 acgacggacg cggctagtag cctctgtcgt gcaaccttga tcgtttcgtc gacgaaccac
     4861 cactgccttg cgccgcggcc ggttgttgcc cagacgccaa cgtccagcgc acctccgcca
     4921 ggatggcagc caaggtgcga ttcggtgatc ccgccgaggg ccagcttgcc gccaagatgc
     4981 acgggaaacc acaccgggtt tgcgcaactc gcccaatcct tgcgaccagg cgcgagccgc
     5041 agcagcgccg tgaagcgagc ttgttcgctc gccggcccgc ccgatcgcgc cgccagtccc
     5101 agtgcattgc gcaagtactg cttcgcagcg cccagtgcga cggtggttat caacgcgcac
     5161 tggtggaagt catccgggca atgcgctggt ccgttcaatg cggcggacat cgaggatgtg
     5221 cattgcgcac tcgggctctt gcaatacatt tgcctgtctg gttcgaggct gtagccatag
     5281 cggagccgaa tgcgtgtctc cggcccccac ttgtcacctc ccaaccactc ttgccattga
     5341 agatccgtca cgttccggaa ggccgggtcg tcggtaacgg tgagcacagc cgggtggccg
     5401 cgaataaggg caagatatcc agttgcgccc tgatcgcgcg tgcacccgcc cagtgagcca
     5461 ctgccacgga ccggtacagt cgcatccgct cgtttttcga acagcaccca ggactggcag
     5521 tcgccggttc cctggacagt gaatgcaccg cccaatccgg ccgacggcag gtattcgacg
     5581 gtgtacggat acgatcggaa aggggccaac tcggcgtgga gcgcggcatc cggaggcgct
     5641 gcaagcgtgt cgatggtgtg ttccgcgtca ctggcgtttc gttcatccgc cacgaccacg
     5701 atgccggccg aacgcagcgc ttcgagcgga tcggtcgcgc cgggcgacaa cgctgcaacc
     5761 gattcgagca atgcccgtgc gaggggcgcg tcctgcgtcg cagccgggtt gtcgatgttt
     5821 cgcaggaatg caatacggag ttggtagaaa tgcgcgagcg tggcttccgc agtgcccgaa
     5881 agtggcgcgg aatcaagatc gtcgcctggt agccacccta tttcacggtt tcgctcaccg
     5941 agccagttga tctcatcctg cctcaggtcg tccgcccgcg atgggtttct tgccagcgcg
     6001 gccgaatacg ccgacgaaac ccgatcatcc aagctcaaga tgtttggatg cgcacaaatc
     6061 gtccggattg cgggatcggg tgcacggctg caatcgattc gcgccgcgaa ggcgggagat
     6121 gccaggaacg cggcggccag cgccagaggc caacagatac gcagttttcg ggattccatc
     6181 agggagatct cactcaagcg gcggcccgca tagagactac catccgcaac agccctggac
     6241 ctccgcgttg aggcgaaacg gtgctcaccc aggcatacat ggaaagcggc gggatacgct
     6301 tcgccgccat ggcccggatg gagattgggg gcaatgggga tcgtcgaagt tcagcgtgcc
     6361 tggatgcttg gtcgatccgc tcgcagcaag catgccccgc gcccctcaat tcactcaaag
     6421 cgacggcttt tcggcctcga gctcccaaag caggtatgga ggttttgccg tggcctgctt
     6481 gccgcgcagc gtgcgcgaaa cggcgcgcaa cttgcgggcc aggcgcttcg ttccggaata
     6541 gttctcggcg gagccccgga tggcgctgta ggcatcgccc cgcagcgtcc agcgcacgtc
     6601 gagggcacga aacctgccca cgaatccgta cacccgcgcg ggcggctcgt cgccgcaaaa
     6661 gtattcgtgg gtgtgcgcag tgatcacgtt gacgtgggtg ggatcggtga aggcttccgc
     6721 gtgcgggtag gcgggggtca gggcgtagaa ccggccgccc ggcgcgagca cccgccagat
     6781 ctcgttcatc agccggatga acggaaagca ggtgccggcc gggccggctg caggcaggat
     6841 tcgcggcacg tgttcgatga aatcgaaggc ggaaaccgag ccgaaatggt catcgtcgaa
     6901 cgggatcggt ccgacggcaa gattggcggc gcggtaatcg aagccgggcg cttccgcgaa
     6961 cgtgaggatg tccacgccgc agagttcccc gcgctggtac gggtttcgcg gccgactgcc
     7021 gcacccgaga tcgaggtggc ggtcgggcag gaccatcaga cttcgaggct cgcgaggtcg
     7081 cctttttctt ccagccagtt gcggcgatcg gacgcgcgct tcttggcgag cagcatgtcc
     7141 atcagttgcg aggtcgcggt atcgtcatcg acagtgagtt gcaccaggcg ccgggtgtcg
     7201 gggtggatgg tcgattcgcg caactgcgcg ggattcattt cacccagtcc cttgaaccgc
     7261 gtgacggaaa ccgcgccctt gatcttctcg cgttcgatgc gtttcagcag cgcgtcgcgc
     7321 tcgccttcat cgaggcagta gaacacctgc ttgcccacgt ccacgcgaaa cagcggcggc
     7381 atcgcgacgc agacgtggcc ttcgcgcacc agcgccggga agtgcttgag gaacagtgcc
     7441 gacagcagcg ttgcaatgtg caggccgtcg gagtcggcgt cggccaggat gatgaccttg
     7501 ccgtagcgca ggccggaaag atcgtccttg ccgggatcgc acccgatcgc gaccgcgagg
     7561 ttgtggactt cctccgaggc cagcaccgcg cccgattcca cttcccaggt gttgaggatc
     7621 ttgccgcgca acgggaggat agcctggtaa tccttgtcgc gcgcctgctt ggccgagccg
     7681 cccgcggaat cgccttccac caaaaacagt tcggtacgat cgggatccga agaggcgcaa
     7741 tcggcgagct tgcctggcag ggccgggcct tgggtgacct tcttgcgcac cacctgcttg
     7801 gccgccttca tccgcgctgc cgcgtgttcg atggcgagtt gcgcgattgc ttcgcccgcc
     7861 gcgacgttct ggttcagcca caacgagaat gcgtcgtgga cgacgccctc ggtcatgccg
     7921 gcggcatcgc gcgaggacag tcgttccttg gtctggccgg cgaattgcgg gtcgcgcatc
     7981 ttgacgctca gcacgtaggc gacgcgttcc cacacgtcct ccggtgacag cttcacgccg
     8041 cgcggcagga ggttccgaag gtcgcagaat tcgcgcagcg cattggtcaa tccggagcga
     8101 aggccgttga cgtgggtgcc gccctggatg gtcgggatca ggttgacgta gctttcctgc
     8161 accagttcgc cttccggtac ccaggcgaac gccgcgtcga ggccaccatc ctcgcgctgg
     8221 gcgtggtgga tgaacagttc cggcggcagg caggtggcgc catcgagctc agacgtcagg
     8281 tattcggcga ggccatcctc gtagcgccac tcctcgcggt cgccgctcgc ttcatcggca
     8341 aggcgcacgg tcaatccggc gcacagtacc gctttcgccc gcagcaggtg cttcaacttc
     8401 ggcagcgcga tcttcggcga atcgaagtac ttcggatcgg gcaggaagcg cagcaccgtc
     8461 ccggtgcggc gcttcgcgac ctcgcccagc gtttcgagtt tgccgatggc ttcgccatcc
     8521 ttgaagcgca tccggtactc ggtgccgtcg cggcggatca gcacttcgag gaacgacgac
     8581 agcgcgttga ccacggatac gcccacgccg tgcaggcctc cagagaaggt gtagttgcgg
     8641 ttcgagaact tggcgcccgc gtgcaggcgg gtcaggatca gttccacgcc cggcaagcct
     8701 tcctccggat ggatgtcgac cggcatgccg cggccatcgt cgctcaccgt gacggagcca
     8761 tccttgtgca cggtcacgtc gatttccttc gcataccctg tcaacgcctc gtcgacggag
     8821 ttgtcgacga cctcctggac gaggtggttc ggacgcgtgg tgtccgtata catgccgggg
     8881 cggcggcgga ccggttcgag accggacagg acttcaatgt cggcggagtt gtagcgggtg
     8941 gagggtgcca tcgggcgcgg atccggggtt cgggaagaca cagccgatca gcatggcgaa
     9001 ccggccggtg ccggtcaatc cataccctgc tggccccgcc ctccggaagc ttcgggaacg
     9061 gctgcaaaaa gccgccagcc tgggccggcc aagccggcag agccgcctcg cccccgatcg
     9121 gcgcaatatt cgaatgcccc gtgttccggg acgcctcttg taaggcgctg ttcgggaagg
     9181 gccgtatacg attgcggctg gacgtgcgcg ccgcggccgc cggctccccg cctcggcgcg
     9241 cattgggcaa agcctgccgg cgcggcggct tccggcctgc gcttgttgcc aactgcctgt
     9301 tgccgttaca ctaatcggct caccgacccc cgagcggggt ttcatccatt ccggcggcgg
     9361 cttgtgcaaa ggcatcgagg tgccgttgca caagccacgt gccgggatct gcttcattca
     9421 acacgctatt catcgagaag gctcccatgc cgctttccac cgaacaaacc agcaagatca
     9481 tcgaagatta tcgccgggca cccaccgaca ccggttcgcc ggaggtacag atcgcgctgc
     9541 tgtccgcgcg catccagcag ctgtccgggc acttcgagtc ccacaagaaa gaccatcact
     9601 cgcgtcgcgg cctgttgaag atggtcaacc atcgccgccg cctgctcggt tacctcaaga
     9661 gcaaggacgc cgagcgttac caggcgctga tcggccgcct cggcctgcgt cgctgagtga
     9721 cgccttccat ttgaactcgc acgccggcag ccgtcgaatc cgaacgctcg ccggctttcg
     9781 gcgtttgctg aacgcgaacg cgcaaaccaa aggacgaatc ccgtggcaaa agtaaccaag
     9841 accttccagt acggccagca cccggtcacc atcgaaaccg gcgagatcgc ccgtcaggca
     9901 agcggtgcgg tggtggtctc gatggccggc accgtggtgc tggtcgcggc ggtcgccgcc
     9961 cccaaggcgc gcgaagggca ggatttcttc ccgctcaccg tcgactacca ggaaaagttc
    10021 tactccgcgg gccgcatccc cggcggcttt ttcaagcgcg agggccggcc gaccgagaag
    10081 gaaacgctga ccagccgcct gatcgaccgt ccgatccgcc cgctgttccc cgaagcgttc
    10141 aagaacgaag tgcaggtggt cgccaccgtc atttcgttga atccggaagt gaatggtgac
    10201 atcccggcgc tgctgggtgc atcggccgcg ctcaccctgg ccggcatccc gttcaagggc
    10261 ccgatcggcg ccgcccgcgt cggctacgtc gacggcaagt acgtgttgaa cccgacggcg
    10321 acggagttgc agtcttccga cctcgacctc gtggtcgccg gcaccgccaa cgcggtgctg
    10381 atggtggagt cggaagccaa gctgctgtcc gaagacgtga tgctgggcgc ggtgacgttc
    10441 ggccacaagg aaatgcaggc cgccgtccgc gcgatcgccg agcttgcgac cgaagtcggc
    10501 aagccgtcgt ggaattggca ggcaccggag cgcgacgcgc aactggtcgc tgccgtgcgc
    10561 ggtgaggtcg gcgacaagct cgccgcggcg ttccaggtca aggacaagct gcagcgccgc
    10621 gatgcgatcg ccgcgctgaa agccgacgtc aaggaagcgc tgaagacgca ggccgaagag
    10681 aagggctggc cggtcgccga catcgtgggt gagttcgagg atctcgaata ccacacggtg
    10741 cgcgacagcg tgctgaagac caggcagcgc atcgacggcc gcgggctgga agacatccgc
    10801 ccgatcaacg tccgtgtcgg cgtcctgccg cgcacccacg gttcggcgct gttcacccgc
    10861 ggcgaaacgc aggccatcgt gaccgtcacc ctgggcaccg gccgcgattc gcagatcatc
    10921 gacgccccgg aaggcgagtc caaggacgcc ttcctgttcc actacaactt ccccccgtac
    10981 tcggtcggcg agaccggacg gatgggcggc cccaagcgcc gcgagatcgg ccacggccgc
    11041 ctcgccaggc gcggcgtgca ggcggtcaag ccgacgatgg aggaattccc gtacgtgatc
    11101 cgcgtggtct cggaaatcac cgaatccaac gggtcgtcgt cgatggcatc ggtgtgcggt
    11161 tcctcgctgg caatgatgga cgcgggcgtg ccgctgaaag cgccggtcgc ggggatcgcg
    11221 atgggcctcg tgaaggaagg cgaggatttc gtggtgctgt c
//
LOCUS       DASSNH010000292        12070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_171388, whole genome shotgun sequence.
ACCESSION   DASSNH010000292 DASSNH010000000
VERSION     DASSNH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12070
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171388"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VFK00_10775"
     CDS             <1..1302
                     /locus_tag="VFK00_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET7593472.1"
                     /translation="QANLRLADVHIAVTGTLTDPMHLGALDMDLRMSADSMSHLYPLT
                     GVILPPTPPFTTHGRLIARIHDGTFRYEDFDGKVGRSDLHGTATYTIAGKRPKLTGHI
                     TSNTLDLADLGPLVGADSNASKAKRGEKRKQPADKLLPIETFDTGRWHQMDADVTFTG
                     KRIVRKAALPIEDLSAHLVLDDARLTLDPVQLGAAGGDIKAHIVLNGREDPMHGKADL
                     SVRHLQLRKLFPTVKMMNTSLGQINGDAALTGDGNSVATLLATSDGEVKLLVNNGTVS
                     KLLLEEAGLNIANIIVTKLAGDEPVQINCAAADLVAKDGLWQSRLFFVDTDTMTIDVD
                     GKLDFRNETLDFTIHPHSKGIRVLSLRSPLYLRGTLKNPKAGVETGPLLARAAGAAIL
                     GTVAAPIAALAAMIAPSHDDDNACVGVMAKMRKPAEAHPGK"
     gene            1508..2422
                     /locus_tag="VFK00_10780"
     CDS             1508..2422
                     /locus_tag="VFK00_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7593473.1"
                     /translation="MSSFTRAHWQLHFCVLLWGITAILGKLITLTALQLVWWRMLIVT
                     LALLLLPKVWRELRGMPPRRIAAYAGIGVLVALHWLTFYSAVKLANASVAATCIALAP
                     VFLALVEPVIVGRRFEPRELLLGIAVVPGVALVVGGVPANMRLGVGVGVLSAVFCATF
                     GAFNKRMAERGGALTVTCLELGAGTLFLTLVTVLWHGEGTFVLPGLRDAALLLVLALA
                     CTLLPFALALAVLRKLSAYTVQLTTNLEPVYAIVLAIPLLGEQHQLDGWFYAGVVLIL
                     GAVFVHPLLVRHPDKPPEAVLGTAESKQ"
     gene            2433..3266
                     /gene="fdhD"
                     /locus_tag="VFK00_10785"
     CDS             2433..3266
                     /gene="fdhD"
                     /locus_tag="VFK00_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017116170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET7593474.1"
                     /translation="MVADKRQARTPPDASEGLRRVDMLHVAPGNLERHADRVIAEAPV
                     ALVYNGVPFAVMMATPCDLEDFALGFALAEGIVESPGEFELVDTLRTDQGISLQGLIP
                     SHRFAALGERHRNLAGRSGCGLCGVESLEEAVRPPPVVTGGPSITVAAVAAGIQAMAG
                     AQPLNAISGGAHAAGFVAGDGRVTVREDVGRHNALDKLIGALASAADSRVDRGFLAIT
                     SRASWEIVHKAAHAGIAIVAAISAPTSLAISAAEAAGITLIGFAREEAMNVYTHARRI
                     A"
     gene            3512..3949
                     /locus_tag="VFK00_10790"
     CDS             3512..3949
                     /locus_tag="VFK00_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7593475.1"
                     /translation="MNPSAKNTIATVIPSLRYRDAPAMIEWLCEAFGFEKHAVYADGD
                     VVQHAQLAFANGMIMLGSVDNATAWGQRIVQPDEIGGRETQACYLVVADCAAHYAQAK
                     AAGAEIVDELESKDYGGSGYSCRDPEGHLWSFGDYDPWAEHAA"
     gene            3946..4788
                     /locus_tag="VFK00_10795"
     CDS             3946..4788
                     /locus_tag="VFK00_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7593476.1"
                     /translation="MKKPDVPIADLLAGARGTPIKVGIGGWNFPPWRDNFYPEELPQR
                     RELEFASRHLATIEVNSTFYRAQTPKVYAKWRDETPPGFVFSLKAPRRVSEVRKLAGA
                     GTSIRGFVFGGLAEFGDRLGPIVWQFPSSTTFERDDFAAFLDLLPQELNGHPLRHALE
                     VRNETFLCETFVELARARGAACVFTDSPEYPNLADLTGDFVYARLKRSREGERNGYPA
                     RELDAWAARARLWQEGGEPDDLPRVSRASGATAMQRDVFIYFISAFKARNPAAAMALQ
                     GRIA"
     gene            4785..6365
                     /gene="dacB"
                     /locus_tag="VFK00_10800"
     CDS             4785..6365
                     /gene="dacB"
                     /locus_tag="VFK00_10800"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET7593477.1"
                     /translation="MKRLGFALAAAMLLAGCASAPPPKPTPAAHPAPITRPASPVPNA
                     ASPRYAPSSLADGIDAYVSQPRFAHAQWGIEVASLDTGRTLYRRNADSLFLPASNAKL
                     YTAALALQTLGPDARFTTALYATAAPRAEGTIAGDLILHGGGDPSLGDPEVSPDWADR
                     LATALANRGVRRVRGDLVADDTWFAGPLHGGGWEAADLQASYGAQSGALGTQDNVVHV
                     KAAREGTRCCTVAIDPPDSGMRVVNLTGGPALLDLYRPPGTDTLYASGSLRADTPRAT
                     FTVAAPDGALLAANQLRDALAQHGIALDGKVRVLHWPETDAALASQPTAIAGITSPPL
                     SQLLVHMLKHSDNRYAQMLLQQVGVRTAQAGACADRIEPPQTSADWGLCAMRAFLARI
                     GVDEGEVTLGDGAGLSRHDLVTPAATVKLLGWIMRQPFANVLRNALPVAGIDGTLKYR
                     MRDDYATDNVQAKTGTLSHVYTLSGFVTDAAGERLVFSLMLNRYVRPTDEFGRNIQPS
                     PQSDLDAIATMLAMHGVN"
     gene            6518..7054
                     /locus_tag="VFK00_10805"
     CDS             6518..7054
                     /locus_tag="VFK00_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593478.1"
                     /translation="MLSSVLVTMALALPAPPASPSLQAVHILELPTRKPRECISIMTG
                     QGMVIVLARPNIERVMSRRSSRAPKAQKRFLELQAQRADALLKGTTEHRDALGCLVNN
                     QLIRNFRPSVQSNWLYVVFALFKEGEGRVWDPATSAFLKKARLVDDDEHCGEGPAGFE
                     ILETLRGEIMASVQTCFR"
     gene            complement(7191..8564)
                     /locus_tag="VFK00_10810"
     CDS             complement(7191..8564)
                     /locus_tag="VFK00_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7593479.1"
                     /translation="MRHDAVTALRAAQVMARLSVFMIAAFAAGAAHASDASVANCHVG
                     AYRLADGSVVDVGPSDDAHLRWRREDGTTGLLTSHADGTWTSTLGWTDRPDGIKVTFS
                     DCAKGRIDFNGGRGDRIAFDVTDTRFRGAGVTLAGRLVLPEGKARVPIVVLVHGSEHF
                     SALDFYALQRLFPAAGIGVFVYDKRGTGASGGTYTQSYLLLANDAIAAVDEAKRLAGP
                     RAGRIGYQGGSQGGWVAPLAAKISPVDFVIVGFGLAVSPLDEDREAIAYDMQRQGYGP
                     DIMAKAMQIADASATILLSNFRSGYDKLDALNRKYGKEPWFKHVHGDVTWILLAWPPE
                     KLKQEGPVLFANVPVQYDPMPVLRNLDTPQLWILGREDADAPSAETIKRLRGLQRAGR
                     PITLAVFPDTEHGIYEFETKPDGTRVDTRNPEGYFAMMRDFILSGRLHGNYGRAVRHG
                     ATADPGR"
     gene            complement(8610..9245)
                     /locus_tag="VFK00_10815"
     CDS             complement(8610..9245)
                     /locus_tag="VFK00_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET7593480.1"
                     /translation="MSHSADLLLFAALVFGIVVLPGLDMAYVMGSSLTAGRRHGLAAV
                     AGIAVGGVCLVVMTTLGIGVLLKVIPAAFNALLLVGALYIAWIGISLLRADSVFGAQA
                     EGCAHSAWTTFRRGTLTCLMNPKAYLFMLAVFPQFLHAGYGPLWSQALVLWIIIAVAQ
                     VTVYGAVALAAAQARGWLVRRPAAGVVTARVVGTVLVGAALFTGLEGWRSL"
     gene            complement(9242..10048)
                     /locus_tag="VFK00_10820"
     CDS             complement(9242..10048)
                     /locus_tag="VFK00_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593481.1"
                     /translation="MSPFVTAAHTTTDTGAALDSVAHLTDYKVVELRRYDIAPGQRDR
                     FVSYFDAYFPEAFEQLDCMVFGQFEDRAAPTKFVWLRGYHDINARPIVDAAFYYGPVW
                     REHRIKVNALFPGESDNVLLLRPLAPLAGVPVLPAVDPVDEPGGARGVVVAQIFAVKK
                     GEEAAFAQRAQAAFRRYDEAKVHPAGVLVTLDVPNNFPQLPIRTDGPFLVWLGVVEND
                     SALEGFETSAAQVEGELEGTDMLRGAPERLVLDPTPRSRLRWWPAPEAKP"
     gene            10172..11227
                     /locus_tag="VFK00_10825"
     CDS             10172..11227
                     /locus_tag="VFK00_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET7593482.1"
                     /translation="MPHPASRVLAVLELLQSRSRISGPELAQRINVDVRTLRRYIVLL
                     EELGIPITTERGRHGAYMLVAGFKLPPMMFTDAEALALSMGLLAARSLGLHAGESALA
                     SAQAKLERVMPDSLKRRLRAVETTVSIESPGVPAAHADTPALMLLTHAAHAAERLHLH
                     YRSAQGEESRRDFDTYGLGFRGGAWYAVGFCHLRRGVRGFRVDRILEARPLPVRFRQP
                     KGFDVLEWLTRTIATLPRAHRVEVLLHADPAKAREAFSPAIGLFETASNGVLLRVRTD
                     DLAWFARQLARVPFEFEVRTPHRLRREILAHAARLQKLATAPAETGSADEPDALPGQP
                     LRPDRPPRTNPIRKRGW"
     gene            complement(11175..11573)
                     /locus_tag="VFK00_10830"
     CDS             complement(11175..11573)
                     /locus_tag="VFK00_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET7593483.1"
                     /translation="MTIALWCVLVAVLLPYVCFGIARNRGRGPDGRRLRDNHDPRDFP
                     NRIHGLAKRAWDAQLNSFESLPVFAAAVIIAHLAHAPQEPVDALAVAWVVARTAYVAF
                     YLANRAALRSAAQFTSLACVLGLFVAAGLA"
     gene            complement(11605..>12070)
                     /locus_tag="VFK00_10835"
     CDS             complement(11605..>12070)
                     /locus_tag="VFK00_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593484.1"
                     /translation="PPLAAEPASASCDAPAYRQFDFFVGDWDTYDVQAPGRVIARNTV
                     KVILDGCVFLEDYRQGDGLHGKSYSLYDAARQVWHQSWVTNRGDLLLLDGGMRGDRMV
                     FEGIERKAGAPDARVRVSWYRQGDGVRETAERSLDGGKTWKIGFDILFKPHM"
BASE COUNT         2016 a         4171 c         3970 g         1913 t
ORIGIN      
        1 caggcgaacc tgcgcctcgc cgacgtgcac atcgcggtga ccggaaccct caccgatccg
       61 atgcacctcg gcgcactgga catggacctc cggatgtccg ccgacagcat gtcccacctg
      121 tacccgctga ccggcgtgat cctgccgccg acgccaccat tcaccacgca cggccggctg
      181 atcgcacgca tccacgacgg cacgttcagg tacgaggatt tcgacggcaa ggtgggcagg
      241 agcgacctgc acggcacggc cacctacacc attgccggca agcgccccaa gctgaccggc
      301 cacatcacgt cgaacaccct cgaccttgcc gacctcggac cactggtcgg cgccgacagc
      361 aacgcgtcca aggccaaacg cggcgagaag cgcaagcagc ccgccgacaa gctgctgccg
      421 atcgaaacct tcgatacggg ccgctggcac cagatggacg cggacgtcac cttcaccggc
      481 aagcgcatcg tccgcaaggc cgccctgccc atcgaggacc tgtcggcgca cctggtgctg
      541 gacgacgcca ggctgaccct cgatccggtg caactgggtg cggccggcgg cgacatcaag
      601 gcccacatcg tgctgaacgg gcgcgaggat ccgatgcacg gcaaggcgga cctgagcgtc
      661 cgccacctgc agctccgcaa gctgtttccg accgtgaaga tgatgaatac cagcctcggc
      721 cagatcaacg gtgacgcggc cttgaccggc gacggcaact cggtggcgac gctgctcgcg
      781 acctcggacg gcgaggtcaa gctgctggtc aacaacggca cggtcagcaa gctcctgctc
      841 gaggaagccg ggctcaacat cgccaacatc atcgtgacca agctggccgg cgacgagccg
      901 gtccagatca actgcgcggc cgcggacctc gttgcgaagg acgggctgtg gcaatcgcgt
      961 ctgttcttcg tggacaccga caccatgaca atcgacgtcg acggcaagct cgacttccgc
     1021 aacgagacgc tggacttcac gatccacccg cacagcaagg gcatccgcgt gctgtcgctg
     1081 cgctcgccgc tgtacctgcg tggcaccctc aagaatccga aagcgggcgt cgagacgggg
     1141 ccgctgctgg cacgcgccgc cggtgccgcg atcctcggca ccgtcgccgc gccgatcgcc
     1201 gcgctggcgg cgatgatcgc gcccagccac gacgatgaca acgcctgcgt cggggtgatg
     1261 gcgaagatgc gcaagccagc cgaagcgcat cccggcaagt gacgtccaag ccaccagcca
     1321 cgtagggtcg aacccgcgca agcggtttcc accgatcggt tccatcggag gcgtcgccaa
     1381 cgcgcagtgg acgcggctgc gccgattcca ccctgcggcg ctgggttgca gcgcccgggt
     1441 tgtgggaaaa tggcgcgcgc ggacggcgtg caatgcgccg tccgttccct tttccggccc
     1501 ggctcccatg tcctccttca cccgcgccca ctggcagttg catttctgcg tgctgctgtg
     1561 gggcatcact gccatcctcg gcaagctgat cacgctgacc gcgctgcaac tggtgtggtg
     1621 gcggatgctg atcgtgaccc tcgcgctgct gttgctgccg aaggtgtggc gggaactgcg
     1681 cgggatgccg ccgcggcgga tcgcggcgta tgccggcatc ggcgtgctgg tcgcgctgca
     1741 ctggctgacg ttctattcgg cggtgaagct cgccaacgct tcggtcgcgg ccacctgcat
     1801 cgcgctggcg ccggtgttcc tcgcgctggt cgagccggtg atcgtcgggc ggcgcttcga
     1861 accgcgcgaa ctgctgctcg gcattgcggt ggtgccgggc gtcgcgctgg tcgtgggtgg
     1921 cgtgcccgca aacatgcggc tcggcgtcgg ggtcggggtg ttgtcggcgg tgttctgcgc
     1981 gaccttcggc gccttcaaca agcgcatggc cgaacgcggc ggcgccctca ccgtgacctg
     2041 cctcgaactc ggggccggca ccctgttcct gacgctggta accgtgctgt ggcacggcga
     2101 aggcactttc gtgctgcccg ggctgcgcga cgccgccctg ctgctggtgc tggcactggc
     2161 ctgcaccctg ctgccgttcg cgctggcgct ggcggtgctg cgcaagctct cggcgtacac
     2221 ggtgcaactg accaccaacc tcgaacccgt gtacgcaatc gtgctggcga ttccgctgct
     2281 gggcgaacag caccagctcg atggctggtt ctacgccggc gtcgtgttga tcctgggcgc
     2341 ggtgttcgtg catccgctgc tggtgcggca tccggacaag ccgccggaag cggtgctggg
     2401 cacggccgag tcgaagcaat aaggtaggca tcgtggttgc cgacaagcgc caagcccgga
     2461 caccgcccga cgcatccgag gggctgcggc gcgtcgacat gctgcacgtg gcgccgggga
     2521 acctcgaacg ccatgcggac cgggtgatcg ccgaagcgcc ggtcgcgctg gtctacaacg
     2581 gcgtgccgtt cgccgtgatg atggcgacac cctgcgacct ggaggacttc gcgctcggct
     2641 ttgcgctggc ggaaggcatc gtcgagtcac cgggcgaatt cgagctggtc gacaccctgc
     2701 gcacggacca gggcatctcg ctgcagggat tgatcccgtc gcaccgcttc gccgcgctgg
     2761 gcgagcgcca ccgcaacctc gccggccgca gcggttgcgg gttgtgcggt gtcgagtcgc
     2821 tcgaggaggc ggtgcgcccg ccaccggtcg tgaccggcgg accctccatc accgtcgccg
     2881 cagtcgccgc gggcatccag gcgatggcgg gggcgcagcc gctcaacgcg atttccggcg
     2941 gcgcgcacgc cgcgggtttc gtggccggcg acggccgcgt gacggtgcgc gaggacgtcg
     3001 gccgccacaa cgcgctcgac aagctgatcg gggcgctggc ctccgcggcg gacagtcgcg
     3061 tggaccgcgg cttcctcgcg atcacctcgc gcgcctcgtg ggagatcgtg cacaaggccg
     3121 cgcacgcggg tatcgccatt gtcgccgcga tttccgcacc cacctcgctg gccatctcgg
     3181 ccgcggaagc cgcgggcatc acgctgatcg ggttcgcgcg cgaggaggcg atgaacgtct
     3241 acacgcacgc acggcgaatc gcctgatccg cgcgatcagg tgcgctcggc cttcttggcg
     3301 tgcttctccg cgcgcttctc cttcagcgac ttctgcggct tcttcttggc ttccttcttc
     3361 ttttcgaggc ctttcatgtc gtgctcccat tggcaatgga ccggcaggtg caaagatgcg
     3421 ccagtccgcg cggcttggca agggggcgcg acggttcctg cggccggccg gaatggctag
     3481 actccctgca ccggcttcga cggagtccga catgaatcca tccgcgaaaa acacgatcgc
     3541 cacggtgatt ccctcgctgc gttaccgcga tgcgccggcc atgatcgagt ggctttgcga
     3601 ggcgttcgga ttcgaaaagc acgcggtgta tgcggacggc gacgtggtgc agcacgcaca
     3661 actcgccttc gccaacggca tgatcatgct gggctccgtg gacaacgcaa ccgcctgggg
     3721 ccagcgcatc gtgcagccgg acgagatcgg cggccgtgaa acccaggcct gctacctggt
     3781 cgtcgccgat tgcgccgcgc actacgcgca ggcgaaggcc gcaggcgccg agatcgtgga
     3841 cgaactcgag agcaaggact acggcggcag cggctacagt tgccgcgatc cggaaggcca
     3901 tctgtggtca ttcggcgact acgatccgtg ggcggaacac gccgcgtgaa gaaaccggat
     3961 gtgcccatcg cggacttgct ggcgggcgcg cgcggtacgc ccatcaaggt cggcatcggc
     4021 ggctggaact ttccgccgtg gcgcgacaac ttctacccgg aggagctgcc gcagcggcgt
     4081 gagctggaat tcgccagccg gcacctcgcg acgatcgagg tcaacagcac gttctatcgt
     4141 gcgcagacac cgaaggtgta cgccaagtgg cgcgacgaaa ccccgccggg attcgtcttt
     4201 tcgctgaagg cgccgcgacg ggtgtcggag gtccgcaaac tcgcgggcgc cggcacgtcg
     4261 atccgcggct tcgtgttcgg cgggctggcc gaattcggcg accgcctggg gcccatcgtg
     4321 tggcagttcc cgtcttcgac caccttcgag cgcgacgatt tcgcggcgtt cctggacttg
     4381 ctgccgcagg aattgaacgg gcacccgctg cgacatgcgc tggaagtacg gaacgaaacc
     4441 ttcctgtgcg agacattcgt cgaactggcc cgcgcacgcg gcgccgcctg cgtgttcacc
     4501 gactcccccg agtacccgaa tctcgcggac ctgaccggcg atttcgtgta cgcgcgcctg
     4561 aagcgcagtc gcgaaggcga gcgcaacggt tatccggcaa gggagctgga cgcctgggcg
     4621 gcgcgtgcac gactgtggca ggaagggggc gagcccgacg acctgccgcg ggtatcgcgg
     4681 gcgagcggcg ccaccgccat gcagcgcgac gtgttcatct atttcatcag cgccttcaag
     4741 gcgcgcaacc ccgcggccgc gatggcgctg caggggcgga tcgcgtgaag cgcctcggct
     4801 tcgcgttggc ggcggcgatg ttgctggccg gctgtgccag cgcaccgcct ccgaagccga
     4861 cgccggcagc gcatccggcg ccgattaccc ggcctgcttc gccggtcccc aatgccgcgt
     4921 cgccacgcta tgcgccctcg tcgctcgcgg acggcatcga cgcctacgtt tcccagcccc
     4981 ggttcgcgca cgcgcagtgg ggaatcgagg tggcttcgct ggacaccggc aggacgttgt
     5041 atcgccgcaa tgccgactcc ctgttcctgc ccgcctccaa cgccaagctc tacaccgccg
     5101 cgctggcgct gcagaccctg ggtccggatg cacgcttcac gaccgccctt tacgcgacgg
     5161 ccgcgccgcg cgcggagggc acgatcgcgg gcgatttgat cctccatggc ggcggcgacc
     5221 cttcgctggg ggacccggag gtgtcgccgg attgggccga tcgcctcgcc accgcgttgg
     5281 caaatcgcgg cgtcaggcgg gtgcgcgggg acctggtcgc cgacgacacc tggttcgccg
     5341 gtcccctgca tggcggcggc tgggaggcgg cggacctcca ggcttcatat ggcgcgcagt
     5401 ccggcgcctt gggcactcag gacaacgtgg tccacgtcaa ggccgcgcgc gagggcacgc
     5461 gctgttgcac ggtggcgatc gatccccccg attccgggat gcgcgtggtg aacctcaccg
     5521 gcggcccggc actgctggat ctctaccggc cgcccggtac cgacacgctg tacgcgagcg
     5581 gcagtctgcg ggcggacacg ccgcgcgcga cctttacggt cgccgcgccg gatggcgcgc
     5641 tgcttgcggc caatcagttg cgcgacgcgc tggcccagca cgggattgcg ctcgatggca
     5701 aggtgcgcgt gttgcactgg ccggaaacgg acgccgcact cgcaagccaa cccacggcca
     5761 tcgcgggcat cacgtccccg ccgctgtcgc aactgctcgt gcacatgttg aagcactcgg
     5821 acaaccgtta cgcgcagatg ctgttgcagc aggtcggcgt ccgcaccgcg caggcgggtg
     5881 catgcgcgga ccggatcgag ccgccgcaga ccagcgcgga ctggggactg tgtgccatgc
     5941 gcgcgttcct cgcacggatc ggcgtcgacg agggcgaggt gactctgggc gatggcgccg
     6001 gcttgtcgcg ccacgacctc gtcacgcccg ccgcgaccgt gaagctgctg ggatggatca
     6061 tgcggcagcc gttcgcgaac gtgctgcgca acgccctgcc ggtcgccggc atcgatggca
     6121 cgctgaaata ccgcatgcgc gacgactatg ccaccgacaa cgtgcaggcc aagaccggca
     6181 cgctgtccca cgtctacacg ctgtcgggct tcgtcaccga cgccgcgggc gaacgcctgg
     6241 tgttctcgct gatgttgaac cgctacgtgc gcccgaccga cgagttcggc cgcaacatcc
     6301 agccgtcccc gcaatcggac ctcgacgcga tcgccacgat gctggccatg catggggtga
     6361 actgaagcaa cgccggcagg catgtgcgga aagcagattc gccggtgtgc cccggcagtt
     6421 tgcaagtttg accggcaaac gggaagatcg gagcggccgt caccctgttg caaggctgtt
     6481 tcagctgaag ccaggtgtcg ccgggcagga gaaatcgatg ctttcgtcgg tcttggtgac
     6541 gatggccctg gcgctacccg cgccacctgc gagtccctca ctgcaggcgg tgcacatcct
     6601 cgaacttccc acgaggaagc cccgcgagtg catttcaatc atgaccggtc aaggcatggt
     6661 catcgtgctc gccaggccga atatcgaaag ggtgatgagc cggagatcaa gtcgggctcc
     6721 caaggcccaa aagcgatttt tggagttgca ggcgcagcgg gctgacgctc tactcaaagg
     6781 aaccacggaa catcgtgacg cgctcggttg cctcgtgaac aaccagctga tccgcaactt
     6841 ccgcccttcg gtgcaatcga attggttgta cgtggtcttt gcgctgttca aagagggcga
     6901 aggccgcgtc tgggatccgg ccacttcagc ttttctgaag aaggcaagac ttgtcgacga
     6961 cgacgagcac tgcggcgagg gtcctgcagg atttgaaata ctcgaaacgc tccgcggcga
     7021 gataatggca agcgtccaaa cgtgcttccg gtagatgtgc aatacatcga agccggccaa
     7081 ccggacctcg acgcgatcgc cacgatgctg gcggtccacg gcgcgaactg acccggcacc
     7141 gacgacgcgc cgcctgtccg gcacgcagcc gcccggcggc aaccagccgt ttacctgccg
     7201 gggtcggcgg tggcgccgtg ccggacggct cgtccgtaat tgccgtgcag gcgcccggaa
     7261 aggatgaagt cacgcatcat cgcgaaatag ccctccgggt tgcgggtgtc gacccgggtg
     7321 ccgtccggct tggtctcgaa ttcgtaaatg ccgtgctcgg tatccggaaa caccgccagc
     7381 gtgatcggcc ggcccgcccg ttgcaggccg cgcaggcgct tgatggtttc cgcgctcggc
     7441 gcgtcggcat cctcccggcc gaggatccag agctgcggcg tgtccaggtt gcgcagcacc
     7501 ggcatggggt cgtactggac cgggacattg gcgaacagga ccggaccttc ctgcttgagc
     7561 ttttccggcg gccacgccag cagtatccag gtgacgtcgc cgtgcacatg cttgaaccac
     7621 ggttccttgc cgtacttccg attcagcgca tccagcttgt cgtagccgct gcggaaattg
     7681 ctgagcagga tcgtggcgga tgcatcggcg atctgcatcg ccttggccat gatgtccgga
     7741 ccgtaaccct ggcgctgcat gtcatacgcg atcgcctcgc ggtcctcgtc gagcggcgac
     7801 accgccagcc cgaaacccac gatcacgaaa tccactggcg aaatcttcgc ggccagcggc
     7861 gccacccagc cgccctggct gccgccctgg taaccgatgc ggccggcgcg cggcccggca
     7921 aggcgcttcg cctcgtcgac cgcggcgatc gcgtcgttgg cgagcaacag ataactctgt
     7981 gtgtacgtgc cacccgaggc gcccgtgccg cgcttgtcgt acacgaacac gccgatgccc
     8041 gcggcgggaa acaaccgctg cagggcgtag aaatcgagcg ccgaaaagtg ttccgagccg
     8101 tgcaccagca ccacgatcgg cacccgcgcc ttgccctcgg gcaataccag ccgcccggcc
     8161 agcgtgacgc cggcgccccg gaagcgggta tcggtgacat cgaatgcgat gcgatcgccg
     8221 cggccgccgt tgaaatcgat gcggcctttc gcgcaatcgg agaacgtgac cttgatgccg
     8281 tccggccggt cggtccagcc cagcgtgctg gtccatgtgc cgtcggcatg cgacgtgagc
     8341 agcccggtcg tgccgtcttc gcgacgccag cgaaggtgcg catcatcgct cggtccgaca
     8401 tccaccacgc tgccgtcggc cagccggtat gcgccgacgt ggcaattggc cacggatgca
     8461 tcggacgcat gggctgcgcc agcggcaaaa gccgcgatca tgaagacgga gagccgcgcc
     8521 atcacctgcg cggcacgtag cgctgttact gcatcatgtc tcatcgcaat tcacttgggc
     8581 gaaacgttcc gtgcattgtc gctgcccact cacaacgaac gccacccctc gagtccggtg
     8641 aacaacgctg cgccgaccaa caccgttccg accacgcgcg cggtgaccac gcccgcggca
     8701 ggccgccgga ccagccagcc gcgtgcctgc gccgccgcaa gcgcgacggc cccgtacacc
     8761 gtgacctgcg cgaccgcgat gatgatccag agcaccagcg cttgcgacca tagcggcccg
     8821 tacccggcgt gcaggaattg cggaaacacc gcgagcatga agagataagc cttcgggttc
     8881 atgaggcagg tcagcgtgcc gcgacggaac gtggtccacg cagagtgcgc gcaaccttcc
     8941 gcctgcgcgc cgaacacgga atcggcacgc agcaacgaga tgccgatcca tgcgatgtac
     9001 aaggcgccga ccagcagcaa ggcattgaag gccgcgggaa tcaccttcag caacacgccg
     9061 atgccaagcg tggtcatcac cacgaggcag acgcccccga cggcgatgcc ggcaacggcc
     9121 gccagcccgt gtcggcgccc ggccgtgagc gaactgccca tcacgtacgc catgtccagg
     9181 cccggcagca ccacgatgcc gaacaccagc gccgcgaaca gcaacaagtc ggccgagtgg
     9241 ctcatggctt ggcttccggt gcgggccacc agcgcaggcg tgaacgcggc gtgggatcca
     9301 gcaccagccg ttccggcgca ccgcgcagca tgtccgtgcc ttcgagttcg ccttcaacct
     9361 gtgccgccga cgtttcaaag ccttccagcg cggagtcgtt ttcgaccaca cccagccaga
     9421 ccaggaacgg accgtcggtg cggatcggca gttgcggaaa gttgttcggc acgtccagcg
     9481 tcaccagcac gcccgccgga tgtaccttcg cctcgtcgta gcggcggaac gctgcctgcg
     9541 cgcgttgcgc aaaggcggct tcctcgccct ttttcaccgc gaagatctgg gcaaccacca
     9601 cgccgcgcgc accgccgggt tcgtccaccg ggtccactgc cggcaaaacc ggcacgccgg
     9661 ccagtggcgc cagcggacgc aaaagcagca cgttgtcgct ctcgcccggg aacagcgcgt
     9721 tgaccttgat ccggtgctcg cgccataccg gcccgtaata gaacgccgca tccacgatcg
     9781 gacgtgcgtt gatgtcgtgg tagccgcgca gccagacgaa cttcgtgggc gcggcacgat
     9841 cctcgaactg cccgaacacc atgcagtcca gttgctcgaa cgcctccggg aaatacgcgt
     9901 cgaaatagct gacgaagcga tcgcgctggc cgggcgcgat gtcatagcga cgcaattcga
     9961 ccaccttgta atcggtcagg tgcgcaacgc tgtccagcgc ggcgcccgta tcggtggtcg
    10021 tatgcgcggc ggtcacgaat ggactcatgc agaaagctcc aagcagcagg actggaagac
    10081 ggggcatctc gatctccggg gcgcgaacgt cgcgaacacg ctaaaaccaa atcagggcga
    10141 tccgtgtcct gattacggat agactggacg catgccccac cccgcctccc gcgtgctggc
    10201 cgtcctggag ctcctgcaaa gccgcagccg catcagcggg cccgagctgg cgcagcgcat
    10261 caacgtcgac gtgcgcacgc tgcggcgcta catcgtgctg ctggaagaac tcgggatccc
    10321 gatcaccacc gagcgcggcc gccacggcgc ctacatgctg gtggctggtt tcaagctgcc
    10381 accgatgatg ttcaccgatg cggaagcgct ggcgctgtcg atgggcctgc tggccgcgcg
    10441 cagcctcggc ctgcacgccg gcgaatcggc actggccagc gcgcaggcca agctggagcg
    10501 ggtgatgccg gattcgctga agcggcggct ccgtgcggtg gaaaccaccg tctcgatcga
    10561 atcgcctggc gtcccggcag cacacgcgga caccccggcg ctgatgcttc tgacgcatgc
    10621 cgcgcacgcc gcggagcgcc tgcacctgca ttaccgttcc gcccagggcg aggaaagccg
    10681 gcgcgacttc gatacctatg gcctgggctt tcgcggcggg gcctggtacg cggtcggttt
    10741 ctgccacctg cggcgcggtg tgcgcggatt ccgcgtcgat cgaatcctcg aggcacggcc
    10801 gctgccggtt cgcttccgcc aacccaaggg tttcgacgtg ctggaatggt tgacccgcac
    10861 tattgccacg ctgccgcgcg cgcaccgcgt ggaggtgctg ttgcacgccg atcctgcgaa
    10921 agcgcgcgag gcgttttcac cggcgatcgg cctgttcgag acggcaagta atggcgtgct
    10981 gctgcgcgtt cgcaccgacg acctggcctg gttcgcgcgc cagctcgcgc gcgtgccctt
    11041 cgaattcgag gtccgtaccc cgcaccggct gcgcagggaa atcctcgccc acgcggcgcg
    11101 gctgcagaag ctcgcgacgg cgcccgccga aaccgggtcc gccgacgagc ccgatgcact
    11161 gccggggcaa ccgctcaggc cagaccggcc gccacgaaca aacccaatac gcaagcgagg
    11221 ctggtgaact gcgccgcgga acgcaacgcg gcccggttgg ccaggtagaa cgccacgtag
    11281 gccgtgcgcg ccaccaccca cgcgaccgcc aacgcgtcga cgggctcctg cggagcgtgc
    11341 gccaggtggg cgatgatcac cgcagccgcg aaaacgggga gcgactcgaa cgaattgagc
    11401 tgcgcgtccc acgcgcgctt cgccaggcca tggatgcggt tcgggaagtc acgtggatcg
    11461 tggttgtcgc gcaatcgtcg gccgtcgggc ccccgtcccc tgttccgggc gatgccgaag
    11521 caaacgtacg gcaacaggac cgccaccagc acgcaccaga gtgcaatggt catttgtctg
    11581 cccctccgat acccgcgcag cgtcctacat gtgcggcttg aacaggatgt cgaacccgat
    11641 cttccaggtc ttgccgccat ccagggatcg ttccgcggtt tcgcgcacgc cgtcgccctg
    11701 cctgtaccag ctcacgcgta cccgtgcgtc gggcgccccg gcctttcgct cgatgccttc
    11761 gaagaccatc cggtcgcccc gcatgccgcc gtccagcagc aacaggtcac cgcgattggt
    11821 gacccagctc tggtgccaga cctggcgggc ggcgtcgtac aggctgtagc ttttgccgtg
    11881 caggccgtcg ccctgccgat agtcctcgag gaacacgcag ccgtcgagga tgactttcac
    11941 ggtgttgcgc gcgatgaccc tgccgggtgc ctgcacgtcg taggtatccc agtcacccac
    12001 gaagaaatcg aactggcggt aggccggcgc atcgcacgac gctgacgcag gctcggccgc
    12061 aagcggcggc
//
LOCUS       DASSNH010000293         7467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_782610, whole genome shotgun sequence.
ACCESSION   DASSNH010000293 DASSNH010000000
VERSION     DASSNH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782610"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="minD"
                     /locus_tag="VFK00_10840"
     CDS             <1..661
                     /gene="minD"
                     /locus_tag="VFK00_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HET7593485.1"
                     /translation="CERRVVYDFVNVIQGEATLKQALIRDKRHDNLYVLAASQTRDKD
                     ALTQDGVGRVLEELAKDGFDYIVCDSPAGIEKGAHLAMYFADRAVVVVNPEVSSVRDS
                     DRILGLLSSKTRRAEKGEGPGPQHLLLTRYNPMRVEAGEMMSVGDVEEILGIPVLGVI
                     PESEQVLAASNSGVPVIHDGSSIAGQAYADAVARLLGEDLPMRFVEAPKKGFFKRMFG
                     T"
     gene            672..947
                     /gene="minE"
                     /locus_tag="VFK00_10845"
     CDS             672..947
                     /gene="minE"
                     /locus_tag="VFK00_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HET7593486.1"
                     /translation="MSLLDFLRPRPKNTASVARERLRIIVAQERATRGGPDYLPLLRN
                     ELLEVIRKYVHVGADAVQINVERNDDGEVLELSVALPEGGKATGTGG"
     gene            1093..1548
                     /locus_tag="VFK00_10850"
     CDS             1093..1548
                     /locus_tag="VFK00_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593487.1"
                     /translation="MRLADIGFEAPAALLGRHGLELVRVEDGAPIPGSYWGEPEAGVI
                     ASNVYARPDTPVHSLLHEACHLLVADPARRASIHTDASDSLEEEDATCYLQIVLADAL
                     PGFGRERALADMDAWGYSFRLGSARAWFEGDAGDASAFLQARGLLPPNA"
     regulatory      1794..1863
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1947..2339
                     /gene="crcB"
                     /locus_tag="VFK00_10855"
     CDS             1947..2339
                     /gene="crcB"
                     /locus_tag="VFK00_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7593488.1"
                     /translation="MLIYALLATGCGAFLGACLRYLFGVWFNPIFPTVPLGTLAANLL
                     GGLLMGLILGAFAQFQSLSPETRVFITTGFLGGLTTFSTFSGETITLLLRQQYGWTFA
                     IIGMHLVGTLAMTLAGFALAQALLRHFA"
     gene            2336..2680
                     /locus_tag="VFK00_10860"
     CDS             2336..2680
                     /locus_tag="VFK00_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7593489.1"
                     /translation="MNDTAPAQGVYLRFFVHENRRHAGVLLYDWLLAEARKLGLPGGT
                     AFRSIAGFGRHGVTHEAHFLELAGQETVRVDFVVGREAADRMLARVREEGLQMFYAIL
                     PAEFGTLGRADA"
     gene            2797..3996
                     /locus_tag="VFK00_10865"
     CDS             2797..3996
                     /locus_tag="VFK00_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7593490.1"
                     /translation="MVKQHAAKPNPARRRALLFVVLLGIVSLFADMTYEGSRSIWGPF
                     LGTLGATGAIVGLVAGGGELLGYVLRLFTGALADKTGRYWAITILGYAINLLAVPALA
                     LAGNWPAAAGLVILERSGKALRTPARDAMLSYAAKDMGGAGWAFGLHEALDSVGAVLG
                     PLIAALVLFLHGGYRHAFAWLLLPALAALATLALARSRFPQPQELDRHPVPEAGDARA
                     LRDLKVFLVATALIAAGYSDFALIAFHLARDHVVASDAVPVLYAIASLAGGATALVLG
                     RWFDRRGLSVLLWATLVPALYAPLVFLGGPWAALAGMVLWGIGFGAHDSLFRAAVAQR
                     IPREKRATAMGVFNAIYGVAWFAGSILLGVLYDWNPLYTVIAALVLQLAACPLLWSLR
                     SRNRSAR"
     gene            3993..5162
                     /gene="cfa"
                     /locus_tag="VFK00_10870"
     CDS             3993..5162
                     /gene="cfa"
                     /locus_tag="VFK00_10870"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HET7593491.1"
                     /translation="MNDMTANTEPAQLSRHDQALREKARKLLDHADVRIDGDREWDLR
                     VHDERVFARTFAHGSLGLGEAYMDGWWDTPSLDQFFYHVLGARLDEHLKNFDALRAHL
                     RARFINMQRGRKAFKIGKAHYDIGNDLFAKMLGRRLVYSCGYWKDATNLDDAEEAKLD
                     LVCRKLQLKPGMKILDIGCGWGEALKYAAEKYGVSGVGVTVSEQQAQFARELCKGLPV
                     EIRLQDYRDLHEPFDAVYSIGMFEHVGWKNYRTYFEVCRRCLKDEGLSVLHCIGVNST
                     PSRPDPWIEKYIFPNSMVPAAAQVTPALENLFVVEDWHNFGADYDKTLMAWLANFDAA
                     WPELSANNPRYDERFRRMWRYYLSGSAATFRTRRDQLWQLTLSPHGVPGGYRVPR"
     gene            5306..5437
                     /locus_tag="VFK00_10875"
     CDS             5306..5437
                     /locus_tag="VFK00_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593492.1"
                     /translation="MLRDGVGVELETKAFDLIPLLVRDHARAPGKRELIEAGHMGRH"
     gene            5476..7269
                     /locus_tag="VFK00_10880"
     CDS             5476..7269
                     /locus_tag="VFK00_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7593493.1"
                     /translation="MNAALVLCSCSAVAVAGNREAGLAAQFKAAAPAERAKLAGNLSH
                     RNDTSEMVAALAPFLESDDAELRADTAYVLRGFYSRALQSAAPALLAAVNHDPAWRVR
                     ANAAYALMTIPETGGTLAAFGKCAGDADARVRAACAKGLGTVAKARQGGDSDVIPLLA
                     TDLGDTARLVRYGAAIGFQELASTEPAAIRALAARLDVEPDPWVRGAIARAIAASGAA
                     AEPALPALTRAVLDPLPLYTASQVVAAMAAIGAPAVPTLIATLGYRGPAETGSSRHAA
                     IAAKAALVKLEVPMTAALIAAVQRGPAQQRMFAAEVLGEKAAEATSAIPALEAALKDD
                     DARVRRAAATSLGQLTTYDNAALPALLGALKDPDSGVRESAVDAVDNGLDGAFVGNDR
                     ALSAARAGKPVPKPVDVLPSPMVAQTAQALGARLGDTDITVAATAAQALGKMGPQAAP
                     ALPPLKAALLAPDGDNARDAADALAAMGGIGAPVLIEAVRKGASPADGSGIGIRGRAA
                     AALATLGWQKQLGDYKAAAVAALIDGLSDPRSDVRGEVAEALGNLGHAGDAPSMKGTV
                     VAALKGALDREQDDDAKQSMQLAMRDWGAGS"
     gene            7266..>7467
                     /locus_tag="VFK00_10885"
     CDS             7266..>7467
                     /locus_tag="VFK00_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593494.1"
                     /translation="MRRFGRGAIGCVILAAIMMASLGMASCSGDQPSGAGDDGSPGDA
                     SAKATLDITASLDGRDVTSDSAG"
BASE COUNT         1204 a         2531 c         2544 g         1188 t
ORIGIN      
        1 ctgcgaacgg cgcgtggtgt acgacttcgt gaacgtgatc cagggcgagg ccacgctgaa
       61 gcaggcgctg atccgcgaca agcgccacga caacctgtac gtgctcgccg cctcgcagac
      121 ccgcgacaag gacgcgctga cgcaggacgg cgtgggccgc gtcctcgagg agttggcgaa
      181 ggatggcttc gactacatcg tgtgcgactc gcccgcgggt atcgaaaagg gcgcgcacct
      241 cgccatgtat ttcgcggacc gcgccgtggt cgtcgtgaac ccggaagtgt catccgtgcg
      301 cgattccgac cgcatcctcg gattgctgtc gtccaagacc cgccgagccg aaaagggcga
      361 aggccccggt ccgcaacacc tgctcctgac ccgctacaac ccgatgcggg tcgaggcggg
      421 ggaaatgatg agcgtgggcg acgtcgagga aatcctcggc atcccggtgc tgggcgtgat
      481 tcccgagtcc gagcaggtat tggccgcatc caactccggc gtgccggtga tccacgatgg
      541 cagctcgatc gccggccagg catacgccga cgcggttgcc cgcctgctcg gcgaggacct
      601 gccgatgcgg ttcgtggagg cgccgaagaa aggcttcttc aagcgcatgt tcggaacctg
      661 aggaggatgc gatgagcctg ctcgactttc tgcgcccgcg cccgaaaaac accgccagtg
      721 tggcgcgcga acggctgcgc atcatcgtgg cgcaggaacg cgccacccgt ggcggacccg
      781 actacctgcc gctgctgcgc aacgagctgc tggaagtgat ccgcaagtac gtccacgtcg
      841 gcgccgacgc ggtgcagatc aatgtggagc gcaacgacga cggcgaggtg ctggagcttt
      901 ccgtggcgtt gccggagggc ggcaaggcga ctggaaccgg cggctaacaa atcatcggtt
      961 gcggcgacag tgcgcccgaa agccatcccc ctcgatcccc cttcgcaggc gaagggggaa
     1021 gacaaaacca cctccttcca caggaaggaa gtcgcgccgc aagcgcgggg ggatgaaacc
     1081 ggaggcgacg tcctgcgcct tgccgacatc ggcttcgagg cgccggccgc cttgctcgga
     1141 cgccacggcc tggaactggt gcgcgtggaa gacggcgcgc cgatccccgg cagctactgg
     1201 ggcgaacccg aagccggcgt gatcgcatcg aacgtgtacg cgcgccctga caccccggtg
     1261 cactcgctgc tgcacgaagc ctgccacctg ctggtcgccg atcccgcacg ccgcgcgtcg
     1321 atccacaccg acgccagcga ttccctcgaa gaggaagatg cgacctgcta cctgcagatc
     1381 gtgctggccg acgcgctgcc cggcttcggc cgcgaacgcg cgctcgccga catggatgcc
     1441 tggggctact cgttccggct gggctccgcg cgtgcgtggt tcgaaggcga cgccggagac
     1501 gccagcgcgt tcctgcaagc gcgcggattg ctgccgccga acgcctaaca gaccttccct
     1561 tcgagaggag gcggccgaag gccgagcgcg tggcgttggc ggggatggcg ttcaatggac
     1621 ggcaaccctg tgggagttgc gacccgcgtt gggctccgct gcgctcagcg ccaacctaca
     1681 agagcacttg tagtcaaatg ctgccgacac gttcgtgcgc gactgccgcc tacgcgcaac
     1741 cgtggcctgt gcgcaggctc ctgcaacgcc acggcggctg ctaccatgcg cgcacagtga
     1801 tgggagatgg catgcctccc cgaaccgtcg ccgtactgtg cgactgatga tgcctgcacg
     1861 accgggattc cggcgcgtcg gcatcgccgc atgacgagcg gccaacgcgt gcggttcccg
     1921 accacctgac gggagcgcac gaatccatgc tcatctacgc cctgctcgcc accggttgcg
     1981 gcgcattcct cggcgcatgc ctgcgttacc tgttcggcgt ctggttcaac ccgatctttc
     2041 cgaccgtgcc gctcggaacg ctggcggcga acctgctcgg cggcctgctg atgggattga
     2101 tcctcggcgc gttcgcccaa ttccagtcgc tgtcgcccga gacgcgggtc ttcatcacca
     2161 ccgggttcct gggtgggctc acgacgttct cgactttttc cggcgaaacg atcacgctgc
     2221 tgttgcgcca gcaatacggc tggactttcg cgatcatcgg catgcacctg gtcggcacgc
     2281 tggccatgac gctcgccggc ttcgcactgg cgcaggcgct gctgaggcac ttcgcatgaa
     2341 cgacaccgca cccgcgcaag gcgtgtacct gcgtttcttc gtgcacgaga accgccgcca
     2401 tgccggcgtg ctgttgtacg actggctgct cgcggaagca cggaaactcg ggctgcccgg
     2461 tggcaccgcg ttccgttcga tcgcgggatt cgggcgccat ggcgtcaccc acgaagcgca
     2521 tttcctcgaa ctcgccggac aggaaaccgt gcgggtcgat ttcgtggtgg gccgcgaggc
     2581 cgccgacagg atgctcgcgc gcgtgcgcga agaaggactg cagatgttct acgcgatcct
     2641 gccggccgaa ttcggcacat tgggccgggc tgatgcctga ctatcggcaa aagcatcttc
     2701 ttgacgcgga tcaacgctga tgaacgcgga tcagcgctgt cgatctgcag tatccgcgtc
     2761 catccgcgtc aagtgccttg tgatcatgca atccgcatgg tgaaacagca cgcggccaag
     2821 ccgaaccccg cgcgccggcg cgcactgctc ttcgtcgtcc tgctcggcat cgtcagcctg
     2881 ttcgccgaca tgacctacga agggtcgcgt tcgatctggg gccccttcct cggcacgctg
     2941 ggcgcgaccg gtgcgatcgt ggggctggtc gccggcggcg gtgaactcct gggctatgtg
     3001 ctccggctgt tcaccggcgc gttggcggac aagaccggac gctactgggc gatcacgatc
     3061 ctcggctacg cgatcaacct gctggcggta ccggcgctcg cgctcgcggg caactggccg
     3121 gcggcggccg gcctcgtgat cctcgagcgc agcggcaagg cgctgcgcac gcccgcgcgc
     3181 gacgcgatgc tttcctacgc cgccaaggac atgggcggcg ccggctgggc cttcggcctg
     3241 cacgaagcgc tggactcggt cggcgcggtg ctgggaccgc tgatcgccgc gctggtcctg
     3301 ttcctgcacg gcggctaccg gcacgcgttc gcatggctgc tgctgccggc gctggccgcg
     3361 ctcgcgaccc tggccctcgc ccgctcgcgc ttcccgcagc cgcaggaact cgaccggcat
     3421 cccgttcccg aggccggtga cgcacgggcg ctgcgcgatc tcaaggtctt cctcgtcgcg
     3481 accgcgctga tcgcggccgg ttattccgac ttcgcgctga tcgcgttcca cctggccagg
     3541 gatcacgtcg tcgcaagcga cgcggtgccg gtcctgtacg cgatcgcgag ccttgccggc
     3601 ggcgccaccg cgctcgtgct cggcaggtgg ttcgaccggc ggggcctgtc ggtactgctg
     3661 tgggcgacgc tggtgccggc gctgtacgca ccgctggtgt tcctcggcgg accgtgggcg
     3721 gcgctcgccg ggatggtgtt gtggggcatc ggcttcggcg cgcacgactc gctgttccgc
     3781 gcggcggtcg cgcaacgcat cccccgcgag aagcgtgcca ccgccatggg ggtgttcaac
     3841 gccatctacg gcgtcgcgtg gttcgcgggc agcatcctgc tcggcgtgtt gtacgattgg
     3901 aatccgctct acacggtgat cgccgcactg gtgttgcagc tcgcggcctg ccccctcttg
     3961 tggagccttc gttcccgcaa caggagtgca cgatgaacga catgacggcg aacaccgagc
     4021 ctgcgcaact gtcgcgccac gaccaggcgc tgcgcgagaa ggcccggaaa ctgctggatc
     4081 acgccgacgt gcggatcgac ggcgatcgcg agtgggactt gcgggtgcac gacgaacggg
     4141 tgttcgcgcg caccttcgcg cacggctcgc tggggctcgg cgaggcctac atggacgggt
     4201 ggtgggacac gccctcgctg gaccagttct tctaccacgt cctcggagct cggctcgacg
     4261 aacacctgaa gaatttcgac gcgcttcgcg cccacctgcg cgcgcgtttc atcaacatgc
     4321 agcgcggccg caaggccttc aagatcggca aggcccacta cgacatcggc aacgacctgt
     4381 tcgcgaagat gctgggcagg cggctggtgt attcgtgcgg gtactggaag gacgcgacca
     4441 atctcgacga cgccgaggaa gccaagctcg acctggtctg ccgcaagctg caactgaagc
     4501 ccggcatgaa gatcctggac atcggctgcg gctggggcga ggcgctcaaa tatgccgccg
     4561 agaagtacgg ggtgtcgggc gtcggtgtca cggtttccga acagcaggcg cagttcgcac
     4621 gcgagctgtg caagggactg cccgtcgaaa tccgcctgca ggactatcgg gacctccacg
     4681 agccattcga cgcggtgtac tcgatcggca tgttcgagca cgtgggctgg aagaactacc
     4741 gcacctattt cgaggtgtgc cgtcgctgcc tcaaggacga gggactttcc gtgctgcact
     4801 gcatcggcgt gaactccacg ccctcccgac ccgacccctg gatcgagaaa tacatcttcc
     4861 cgaactcgat ggtcccggcc gcggcgcagg tcacgcccgc gctcgagaac ctgttcgtgg
     4921 tcgaggactg gcacaacttc ggcgccgatt acgacaagac attgatggcg tggctggcca
     4981 atttcgatgc cgcgtggccg gaactgtccg cgaacaatcc ccgctacgac gaacggttcc
     5041 gccggatgtg gcgttactac ctctcgggat ccgccgccac cttccgcact cgccgcgacc
     5101 agctttggca actcacgctg tcgccgcacg gcgtgccggg cggctaccgc gtgccgcgct
     5161 gagggcccct gccaactgcc ggtttgtggt acacaaggcg ttccggggat cgatgcaagg
     5221 ccgttcgggg tggcttgcat gcaagacgat gtaggaacac gggggtccac agcgacgttt
     5281 gcgtgatcca gccagcatcg ggggcttgct gcgcgacggt gtgggcgtcg agctggagac
     5341 caaggccttc gacctgatcc cgttgctggt gcgcgaccac gcgcgtgcgc ccggcaagcg
     5401 ggaactgatc gaggctggac acatggggcg gcattgaagg ggtgaacacc agtgggaaac
     5461 aagcggatac tgatcttgaa tgccgcactg gtgctgtgct catgcagcgc cgtcgcggtc
     5521 gcgggcaatc gcgaagccgg gctggccgcg cagttcaagg ccgcggcccc ggcggagcgt
     5581 gccaagctcg ccgggaacct gagccatcgc aacgacacct ccgagatggt cgccgcgctg
     5641 gcaccgttcc tggaaagcga tgatgccgag ctgcgcgcgg acacggctta cgtgctgcgc
     5701 ggcttctaca gccgcgccct gcagtcggcc gcccctgcgc tgctggccgc ggtgaaccac
     5761 gatccggcat ggcgggttcg cgccaacgcg gcgtatgcgc tgatgactat ccctgaaacc
     5821 ggcggcacgc ttgccgcgtt cggcaaatgc gccggcgatg ccgacgcgcg ggtgcgcgca
     5881 gcctgcgcca agggactcgg caccgtcgcc aaggcccggc aaggtggcga cagcgatgtg
     5941 atcccgttgc tcgccaccga cctcggcgac accgcgcggc tcgtccgcta cggcgccgcg
     6001 atcgggttcc aggaactcgc atccaccgag cccgccgcga tccgcgcgct ggcggcgcgc
     6061 ctcgacgtcg agcccgaccc gtgggtccgc ggcgccatcg cccgagccat tgcggcatcg
     6121 ggcgcggccg ccgaaccggc actgccggca ctgacgcgcg ccgtgctcga tccgttgccg
     6181 ttgtacacgg cgagccaggt ggtcgccgcg atggccgcga tcggtgcacc ggccgtgccc
     6241 acgctcatcg cgacgctggg ctacaggggg ccggcggaga cggggtcctc gcgtcacgca
     6301 gcgatcgctg cgaaggcggc actggtgaag ctcgaggtac cgatgaccgc ggcgctgatc
     6361 gccgccgtgc aacgcggccc ggcccagcag cgcatgttcg cagccgaggt actcggtgaa
     6421 aaggccgcgg aagccaccag cgcgatcccc gcactggaag ccgcgctgaa ggacgacgac
     6481 gcccgcgtcc gccgggctgc ggcaacatcg ctcggtcagt tgacgactta cgacaacgca
     6541 gcgttgcccg cactgctcgg cgcgttgaag gatcccgact ccggcgtgcg tgaatccgcg
     6601 gtcgatgcgg tggacaatgg cctggatggc gcgttcgtcg gcaacgatcg cgcgctgtcc
     6661 gcagccaggg ccggcaaacc ggtgccaaaa cccgtggacg tcttgccctc gcccatggtt
     6721 gcgcaaacgg cccaggcact cggcgcccgt ctcggcgaca ccgacatcac cgtcgcggcc
     6781 accgcggcac aagcgctcgg gaaaatgggc ccgcaagccg cgccggcact gccgcccctc
     6841 aaggcggcat tgctcgcgcc ggatggcgac aacgcgcgcg atgccgccga cgcgctggcg
     6901 gcgatgggcg gcatcggcgc acccgtgttg atcgaagcgg tccgcaaggg tgcatcgccg
     6961 gccgacggca gcggcatcgg cattcgcggc cgtgccgcgg ccgccctggc gaccctgggc
     7021 tggcagaagc aactcggcga ctacaaggct gcagcggtgg ccgcgttgat cgacgggctt
     7081 tccgatcccc gcagcgacgt gcgcggcgag gtcgccgagg cactcggcaa cctgggacac
     7141 gcaggtgacg ccccgtccat gaagggcacg gtcgtggcgg cgctcaaggg cgcgctggac
     7201 cgggagcagg acgacgatgc aaagcaatcc atgcaactgg caatgcgaga ctggggagcg
     7261 ggttcatgag acgtttcgga cggggcgcga tcggctgcgt gatccttgca gccatcatga
     7321 tggcttcact gggcatggcg tcatgttccg gtgatcaacc gagcggtgcc ggcgacgacg
     7381 gcagccccgg cgacgcctcg gcaaaggcca cgctcgacat caccgcgtcg ctggatggcc
     7441 gggacgtgac gagcgacagc gccggga
//
LOCUS       DASSNH010000294         4576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1558948, whole genome shotgun sequence.
ACCESSION   DASSNH010000294 DASSNH010000000
VERSION     DASSNH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558948"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /gene="purH"
                     /locus_tag="VFK00_10890"
     CDS             <1..1389
                     /gene="purH"
                     /locus_tag="VFK00_10890"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7593495.1"
                     /translation="MDGRVKTLHPKVHGGLLGRRGTDEAVMRDLAIAPIDLLVVNLYP
                     FARTVANAGTTYDEAIEEIDIGGPAMLRAGAKNHERVAVVCDPADYPDLIETLRRDGG
                     TSFEQRRRLAARVFAHTARYDGMIADWMAARAEGEPTEAYPPTLHLALRRAQELRYGE
                     NPHQAAALYVDGNVSAGTVAGASVLAGKEMSYNNLVDADTALECVKAFDKTPACVIVK
                     HANPCGVALGDDPIDAYGRAFACDSTSAFGGIIAFNRPIDGELVRRILANQFVEVLVA
                     PAFDEAALAALRKKPNVRALATGAWPVSPGVAQRQVRSVAGGMLVQDSDIDTLMLSDL
                     KVVTRRVPDAEQLRDLLFAWHVAMFVKSNAIVYAKSGATIGIGAGQMSRVISVRIAAM
                     KAADAKLSTDGSVMASDAFFPFRDNIDLAAGTGVRAVIQPGGSMRDQEVIDAADEHGM
                     AMVFTGTRHFRH"
     gene            1606..2337
                     /locus_tag="VFK00_10895"
     CDS             1606..2337
                     /locus_tag="VFK00_10895"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET7593496.1"
                     /translation="MRYELYYWPTTQGRGEFVRLALEDAGADYVDVARLPEGSGGGEA
                     ALLHWLEEPPVGHPSFAPPFLKAGRNLIGQTANILLYLGPRLGLAPADESGRLWTHQL
                     QLTITDLVAEIHDAHHPIAGSLYYDDQRDEAKRRSAHLRRERLPKYLGWFERVLAENR
                     RGRQRCLVGSAVTCADLSLFQVIAGLRYAFPKAMRRLEADLPLCVDLHDRIGGRPNIA
                     AYLASPRRIPFNNDGIFRRYPELDG"
     gene            complement(2399..2884)
                     /locus_tag="VFK00_10900"
     CDS             complement(2399..2884)
                     /locus_tag="VFK00_10900"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HET7593497.1"
                     /translation="MKTQAWWILATAVLAMPAAHAQNVLAASSAPPPPGSALPPPGIN
                     DKGTKPQAVPLPDTGILPSVAPAARQDANGQTSGVSVSTNANGDTIEEYRRGGRVYMV
                     RIIPKNGVTQTYHYDSTNGSLVHDPKLGPVSPVYYDIYKWGKPSQPASGAPAEGSTSG
                     G"
     gene            2975..>4576
                     /locus_tag="VFK00_10905"
     CDS             2975..>4576
                     /locus_tag="VFK00_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET7593498.1"
                     /translation="MPKLTLIDGSSYLYRAFHALPPLSNAAGEPTGALFGVVGMLRAI
                     LNDNPDFAAFVIDAPGRTFRDDLYAAYKATRAPMPDDLRSQVAPMLAIVEALGFPILN
                     VPGVEADDVIGTLAQRAAADGIDVEVSTSDKDFAQLVGPRVTLVNTMTRSSLDRAGVI
                     EKFGVPPERIVDYLALMGDSIDNIPGVAKCGPKTAAKWLNQYGTLDDVIARASEVGGK
                     IGENLRAALEQLPLSRQLATIRTDVALEAGPRDLLLRARDIDALRELYTRYEFRQALK
                     ELEGAGPGTAIGSATDHVEGTIEGKADPATSTRPTPGPQSPVPGPAEYDLVTTRGQLD
                     SWLAKLHSADLIAFDTETTSLDPMQAELVGLSFAVEAGRACYIPIGHDCTGVPQQLAL
                     GELLASLKPILEDPQRPKLGQHAKYDSNVLSHYGIVVRGIEFDTMLESYVLNATASRH
                     DMDTLARRWLAYDTVHYEDICGKGAKQIPFSQVDLDTACRYAAEDADITLRLHQALWP
                     QLQAEPGLQKVFTEIEMPLVPVLARM"
BASE COUNT          765 a         1590 c         1494 g          727 t
ORIGIN      
        1 atggacgggc gggtcaagac gctgcacccc aaggtgcacg gcggcctgct ggggcgccgc
       61 ggcaccgacg aagccgtgat gcgcgacctc gcgatcgcgc ccatcgacct gctcgtcgtc
      121 aacctctacc cgttcgcgcg caccgtggcc aacgccggca ccacctatga cgaggccatc
      181 gaggaaatcg acatcggcgg tcccgcgatg ttgcgcgctg gcgccaagaa ccacgaacgc
      241 gtggcggtgg tctgtgaccc cgccgactat ccggacctga tcgaaaccct gcgccgcgat
      301 ggcggcacgt ccttcgaaca gcgccggcgc ctcgccgcgc gcgtgttcgc gcacaccgcc
      361 cgctacgacg gaatgatcgc cgactggatg gcggcgcgtg ccgaaggcga acccacagaa
      421 gcatacccgc cgacgctgca cttggcgttg cgacgcgcgc aggaacttcg ctatggcgag
      481 aatccccacc aggcggcggc gttgtatgtc gatggcaacg tgtcggccgg cacggtcgcc
      541 ggcgccagcg tgctggccgg caaggaaatg tcctacaaca acctcgtcga tgccgatacc
      601 gcgctggaat gcgtcaaggc gttcgacaag acgcccgcct gcgtgatcgt caagcacgcg
      661 aacccctgcg gcgtcgcgct gggcgacgat ccgatcgatg cgtatggccg cgccttcgcg
      721 tgcgactcga cgtcggcgtt cggcggcatc atcgccttca accgcccgat cgacggggaa
      781 ctcgtccggc gcatcctcgc caaccagttc gtcgaggtgc tggtggcgcc tgcattcgac
      841 gaggcggccc tcgccgcgct ccgcaagaag ccgaacgtgc gcgcgctggc caccggcgca
      901 tggcccgttt cacccggcgt cgcgcaacgc caggtccgca gcgtcgcagg cggcatgctg
      961 gtccaggatt ccgacatcga cacgttgatg ctgtccgacc tcaaggtcgt cacccggcgc
     1021 gtgcccgacg ccgagcagtt gcgcgacctg ttgttcgcct ggcatgtcgc gatgttcgtg
     1081 aagtcgaacg cgatcgtgta cgcgaagagt ggcgccacca tcggcatcgg cgccggacag
     1141 atgagccggg tgatcagcgt gcggatcgcc gcaatgaaag ccgccgacgc caagctttcc
     1201 accgacggca gcgtaatggc gtcggacgcg ttcttcccct tccgcgacaa catcgatctt
     1261 gccgcaggca cgggcgtgcg cgccgtgatc cagcccggcg gatcgatgcg cgaccaggaa
     1321 gtcatcgacg cggccgacga gcacggcatg gcgatggtgt ttaccgggac ccggcatttc
     1381 cgccattgag ttgaaagcgg gaatggggaa cagggaatgg ggaggccggg ttccacgacc
     1441 ttggccgggg ccagagtgcc tccggtcgcc gcgtcgcacc atccgacgca ttgatcgaaa
     1501 gcgccaggtt cacagtggag atgctgccgc cacgggatcc ggccactccg ttggagtagt
     1561 ctttgtcccg gttcccggtt cccggttccc tgttcccgct ccacgatgcg ctacgagctc
     1621 tactactggc ccacaaccca gggacgcggc gagttcgtgc ggctcgcgct cgaagacgcc
     1681 ggcgcggatt acgtcgacgt ggcgaggctg ccggaaggct cgggtggcgg cgaagcagcg
     1741 ctgctgcact ggctggaaga accgccggtc ggccatccct cgttcgcgcc gccgttcctc
     1801 aaggccgggc gcaacctgat cggccagacc gccaacatcc tgctgtacct cggtcctcgg
     1861 ctcggactcg cgcccgctga cgaaagcggg cggctgtgga cccaccagct gcagctgacg
     1921 atcacggacc tcgtggccga gatccacgat gcccaccacc cgatcgcggg cagcctttat
     1981 tacgacgacc agcgcgacga agcaaagcgc cgcagcgcgc acctccggcg cgagcggttg
     2041 ccgaaatacc tcggctggtt cgagcgcgtg cttgcggaga accgccgcgg gcgccagcgt
     2101 tgcctggtcg gcagcgccgt cacctgcgcc gacctgtcgc tgttccaagt catcgccggc
     2161 ctgcgttacg cgttccccaa ggcgatgcgc cgcctcgaag ccgacctgcc gctgtgcgtg
     2221 gaccttcatg accggatcgg cgggcggccg aacattgcgg cctatctcgc atcaccgcgg
     2281 cgcatcccct tcaacaacga cggcatcttc cgtcgctacc cggaactgga cgggtgaggc
     2341 gacgcaaggc caaggatggc cgggccggcg cccagcacca tgcacggttc cccgatgact
     2401 acccgccgct cgtcgaccct tcggcaggcg cgccactcgc gggctggctc ggcttgcccc
     2461 acttgtagat gtcgtaatac accggcgaca ccgggccgag cttggggtcg tgcaccaggc
     2521 tgccgttggt gctgtcgtag tggtaggtct gggtgacgcc gttcttggga atgatgcgca
     2581 ccatgtagac ccgcccgccg cggcgatatt cctcgatcgt gtcgccgttg gcattggtgc
     2641 tcacgctcac gccggaggtt tgtccgttgg cgtcctggcg cgcggccggt gcaacggacg
     2701 gcaggatacc ggtgtccggc agcggtaccg cctgcggttt cgtgcccttg tcattgatgc
     2761 cggggggcgg caaggccgat ccgggtggcg gcggtgccga ggacgccgcc aagacgttct
     2821 gcgcgtgcgc ggccggcatc gccagaaccg cggtcgcaag gatccaccac gcctgcgttt
     2881 tcacggcctg cctccgtcgt ttttcgcctg cgtgcagcat acccgggtgt ggcgtgaccg
     2941 cggcgtgcag ggaggtacat gcgacaatcg gcggatgccc aaactgacac tcatcgacgg
     3001 gtccagttac ctgtaccgtg ccttccacgc gctgccgccg ctcagcaatg ccgcgggaga
     3061 gccgaccggc gcactgtttg gcgtggtcgg catgctccgg gcgatcctga acgacaaccc
     3121 ggatttcgca gcattcgtga tcgatgcgcc gggccggacc ttccgcgacg atttgtatgc
     3181 cgcgtacaag gccacccgcg caccgatgcc ggacgacctg cgttcgcagg ttgcgccgat
     3241 gctcgcgatc gtggaagcgc tggggtttcc gatcctcaat gtcccgggcg tggaggccga
     3301 cgacgtgatc ggcaccctcg cacagcgtgc cgcggcggac ggcatcgacg tggaagtctc
     3361 gacgagcgac aaggatttcg cgcaattggt cggtccccgc gtcacgctgg tcaacaccat
     3421 gacccgttcc agcctcgacc gcgccggcgt gatcgaaaag ttcggcgtgc cgccggagcg
     3481 gatcgtcgat tacctcgccc tcatgggcga cagcatcgac aacatccccg gcgtcgccaa
     3541 atgcggcccc aagaccgcag ccaagtggct gaaccagtac gggacgctcg acgacgtcat
     3601 tgcgcgcgcc agcgaggtcg gcggcaagat cggtgagaac ctgcgcgcgg cgctggagca
     3661 actcccgcta tcgcggcagc tcgccacgat ccggaccgac gtcgcgctgg aagcgggtcc
     3721 gcgcgacctc ctgttgcgcg cgcgagacat cgacgcactg cgcgagctgt ataccaggta
     3781 cgaattccgg caggcgttga aggagctgga aggcgcggga ccgggaaccg ccatcggttc
     3841 cgcaaccgac cacgtcgagg gcaccatcga aggcaaagcc gacccggcga caagcacccg
     3901 gccaacgccc ggtccccagt ccccggtccc cggtcccgca gagtacgacc tggttaccac
     3961 tcgagggcag ctcgacagtt ggctcgcgaa actccattcc gccgacctga tcgccttcga
     4021 taccgaaacc acctcgctcg atccgatgca ggccgaactc gtggggctgt cgttcgcggt
     4081 ggaagccggc cgtgcctgct acatccccat cggccacgac tgcacggggg tgccgcagca
     4141 gcttgccctc ggggaactgc tcgcttcgct gaagccgatc ctcgaggatc cgcagcggcc
     4201 caagctgggt cagcacgcca agtacgacag caacgtgctg tcgcactacg gcatcgtggt
     4261 gcgcgggatc gagttcgaca cgatgctgga atcctacgtg ctgaacgcga ccgcgagccg
     4321 ccacgacatg gacacgctcg ccaggcgctg gctcgcctac gacaccgtcc attacgagga
     4381 catctgcggc aagggcgcca agcagatccc cttctcgcag gtcgacctcg acactgcctg
     4441 ccgctacgcc gccgaggacg ccgacatcac gttgcggctg caccaggcgc tctggccgca
     4501 gctccaggcc gagcccggct tgcagaaggt gttcaccgag atcgagatgc cgctggtccc
     4561 ggtactcgca cggatg
//
LOCUS       DASSNH010000295         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_446076, whole genome shotgun sequence.
ACCESSION   DASSNH010000295 DASSNH010000000
VERSION     DASSNH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446076"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..908)
                     /gene="cmk"
                     /locus_tag="VFK00_10910"
     CDS             complement(225..908)
                     /gene="cmk"
                     /locus_tag="VFK00_10910"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7593499.1"
                     /translation="MTIPHSVPVLTIDGPSGSGKGTIARKVADQLGWHILDSGAIYRA
                     VGYAASAAGVDPGDAEAVARLAGATRLEFLDPGDGGDTRVIIDGTDATDAIRTETAGA
                     AASRIAAQTAVREALVAKQLAFRRPPGLVADGRDMGTVIFPDAPYKVFLTASAGERAE
                     RRYKQLKAKGLQVTLASLLHEIEVRDARDASRTVAPLRAAADAVVIDTTGRSIAAVVD
                     AVLALVHKD"
     gene            complement(938..2248)
                     /gene="aroA"
                     /locus_tag="VFK00_10915"
     CDS             complement(938..2248)
                     /gene="aroA"
                     /locus_tag="VFK00_10915"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7593500.1"
                     /translation="MKARLDWATSPAGPLHGDVVLPGDKSVSHRAIMLSALAEGTSRI
                     TGFLEADDTGATARIFGQMGVHIETPAANERIVHGVGLRGLRAPEGVLDCGNSGTAVR
                     LLCGILAGQSFDSVLAGDDSLSKRPMRRVTDPLAAMGAGLETTEGGTPPLRVHGGRRL
                     RGIDYASPIASAQVKSAVLLAGLYAEGETVVTEPRPTRDYTERMLEAFGWPVAFGPGR
                     ARLSGGHALQATDVAVPADFSSAAFFIVAATLVPGSELRLRNVGMNPRRTGLLQVLRA
                     MGANIAQENARSAGGEAVADLVVRYAPLHGIEVPVELVADVIDEFPILFVAAACAEGT
                     TTIRGAAELRVKESDRIAVMAAGLRQLGVAIDESPDGAMIEGGRLQGGNVDAQGDHRC
                     AMAFAMAGAVAGGAITIRDCANVATSFPGFVTLAQACGLDVRPA"
BASE COUNT          363 a          795 c          758 g          356 t
ORIGIN      
        1 atgccggata cgatcgatcc gggtttcaat ttggccagcg actggctctg ttcaaacagc
       61 tcggcaaaac tttcggtcat ggggattcca ggttgggaat ggaccgcgag gccttgaagt
      121 tccatgtaac gcttgctcca caggcggcgg tccgccggtt gggggatgca tcggcatccg
      181 ttcgagtaac gccgcgcgga cgcggcacgg ggtgaatcgt tcgatcagtc cttgtgcacg
      241 agcgcgagca ccgcgtcgac caccgcagca atcgaccgtc cggtggtatc gatcaccacg
      301 gcatcggcgg ccgcacgcaa cggcgcgacc gtgcgcgacg catcccgtgc gtcccgcacc
      361 tcgatctcgt gcaaaaggct ggctagtgta acttgaagtc cctttgcctt caactgctta
      421 tagcgccttt ccgcgcgctc gccggcgctg gccgtcagga acaccttgta gggcgcatcg
      481 ggaaagatca cggtgcccat gtctcgcccg tccgcgacca gccccggagg ccggcggaat
      541 gccaactgct tggccacgag ggcctcgcgg accgcggtct gggccgcgat ccgcgaggcc
      601 gccgcgccgg cggtttcggt gcggatcgcg tcggtggcgt ctgtgccgtc aatgatgacc
      661 cgggtgtcgc cgccgtcgcc gggatcgagg aattccagcc gggtcgcgcc ggcgaggcgc
      721 gccacggcct cggcgtcccc cggatcaacc ccggcagcgc tggcggcgta gccgaccgcc
      781 cggtagatcg cgcccgaatc gaggatatgc cagcccagtt ggtcggccac cttacgggcg
      841 atcgtgccct tgcccgaacc ggaggggccg tcgatggtga gtaccgggac ggaatgggga
      901 atggtcatcc tcgggatttt agcggctggc gaaccggtca ggccgggcgc acgtccaatc
      961 cgcaggcttg tgccagtgtg acgaagcccg gaaaggacgt cgcgacgttc gcacaatccc
     1021 ggatcgtgat ggcgccgccg gccaccgcac cggccatcgc gaatgccatc gcgcaacggt
     1081 ggtcgccctg cgcatccacg ttgccgccct ggaggcgccc gccttcaatc atcgcgccgt
     1141 ccggtgattc gtcgatggcg acgccgagtt ggcgaaggcc cgccgccatc acggcgatgc
     1201 ggtcggattc cttgacccgc agttccgctg caccacggat cgtggtcgtg ccttcggcac
     1261 atgccgcggc gacgaacagg atcgggaact cgtcgatcac gtcggcgaca agctccaccg
     1321 gcacctcgat gccgtgcagg ggtgcgtacc gcaccaccag gtcggccacc gcttcgcccc
     1381 ccgcgcttcg ggcattttcc tgcgcgatgt tggcacccat ggcgcgcagc acctgcagca
     1441 aaccggtgcg ccgcgggttc atgccgacgt tccgcagccg aagttccgaa cccggcacca
     1501 gggtcgcggc cacgatgaag aaggcggccg aggaaaaatc cgcgggcacg gccacatcgg
     1561 tcgcctgcag cgcgtggccg ccggaaagcc gcgccctgcc cggcccgaac gcgacgggcc
     1621 agccgaatgc ctccagcatc cgttcggtgt agtcgcgggt ggggcgcggc tcggtcacga
     1681 cggtctcgcc ctccgcgtac aggcccgcca gcaacacggc ggatttcacc tgagcgctgg
     1741 cgatgggcga tgcgtagtcg atcccgcgca agcgacgtcc gccgtgcacg cgcagcggcg
     1801 gcgtgccgcc ctcggtcgtt tcgaggcccg ctcccattgc cgccagcgga tcggtcacgc
     1861 ggcgcatcgg ccgcttcgaa agcgagtcat cgcctgccag cactgaatcg aacgactgcc
     1921 ccgccaggat gccgcacaac aggcgcaccg ccgtgccgga attcccgcag tcgagcacgc
     1981 cctcgggcgc gcgcaaaccg cgcaggccta cgccgtgcac gatgcgctcg ttcgcggcgg
     2041 gcgtttcgat gtgtacgccc atttgcccga agatgcgggc cgtcgcgccg gtgtcgtcgg
     2101 cttccaggaa tcctgtaatc cgcgatgtcc cctccgccag cgcggacaac atgatcgcgc
     2161 gatgcgaaac cgacttgtcg ccgggcagga cgacgtcgcc gtgcaatggg cccgccggag
     2221 acgtggccca atcgagccgt gctttcacgg gagggcccgc aacagccgtc ga
//
LOCUS       DASSNH010000296        16172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_42829, whole genome shotgun sequence.
ACCESSION   DASSNH010000296 DASSNH010000000
VERSION     DASSNH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 16172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16172
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42829"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VFK00_10920"
     CDS             complement(<1..107)
                     /locus_tag="VFK00_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HET7593501.1"
                     /translation="MRRADRLFLIIHALQGRRTALPARRLADTLGVSLRT"
     gene            complement(118..591)
                     /locus_tag="VFK00_10925"
     CDS             complement(118..591)
                     /locus_tag="VFK00_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7593502.1"
                     /translation="MEFRDDGERIYVLWDRYARERDVEGLLSLYAEDAILETPLIPAI
                     LGVERGALEGHAALRHFFEEGGRRRPNELVRWHREEGRYFFDGSRLTWEYPRAAPDGD
                     QVDLVEVMDLAGGMIRHHRIYWGWFGVGQLVRSALSRHTRKQQQALAAGRPEGDR"
     gene            complement(615..1250)
                     /locus_tag="VFK00_10930"
     CDS             complement(615..1250)
                     /locus_tag="VFK00_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7593503.1"
                     /translation="MPPPSDLVFFHNPHSRAAMTRALLEELGAKYEPCIVDFRRHEQL
                     SPEYAAINPMGKVPAIRHDGVVVTETVAIFIYLADLYREAGLAPALDDPDRGSYLRWM
                     VFYAACFEPAIGDKAMKREPAPRAQSPYADYDTTVAAITQALEPGPWLLGERFSAADV
                     LWGNALRWVTGFGMVEATPVIADYIERVMARPAEQRALEADAVLAAEMGLG"
     gene            1378..2400
                     /locus_tag="VFK00_10935"
     CDS             1378..2400
                     /locus_tag="VFK00_10935"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HET7593504.1"
                     /translation="MQRDLQGHVSLTQHLIEEQRRHDSVNAELRLLIEVVARACKRIS
                     IAVGKGALGGVLGDAGTGNVQGEAQKKLDVISNEILLEANAWGGHLAACASEEMAEPY
                     PIPDAYPKGNYLLLFDPLDGSSNIDVDISVGTIFSVLRCPEGVTHPGERDFLQPGTRQ
                     VAAGYCVYGPSTLLVLTTGQGVNVFTLDREIGSFRLTRANARIPEDTSEFAINMSNQR
                     FWEAPMQRYIADLLAGKTGPRGRDFNMRWVASMVADVHRILTRGGIFIYPLDEKVKDK
                     GGKLRLMYEANPMSFIVEQAGGAATTGRSRILDVQPTQLHQRVPVFLGSRNEVETATK
                     YHLEDA"
     gene            complement(2519..3199)
                     /locus_tag="VFK00_10940"
     CDS             complement(2519..3199)
                     /locus_tag="VFK00_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7593505.1"
                     /translation="MSLAGKRIAITGAFGSLGAAVVQAALAAGAQVAAIDRADPPADT
                     HGARAIGGVDLADAAQAKAAIDDAAKALGGLDALVNIAGTFRFETLADGSPDTWDLLY
                     RINVRTAVAASKAALAHLPEGGRIVNIGAASALKAGAGVGAYTASKAGVLRLTESLAE
                     ELKPRGITVNAVLPSIIDTPPNRRDMPKADFAKWVKPAQLADVIVFLLSDQASAITGA
                     LIPVTGRV"
     gene            3567..4448
                     /locus_tag="VFK00_10945"
     CDS             3567..4448
                     /locus_tag="VFK00_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7593506.1"
                     /translation="MASFPEDAIPPSPAAVRPARTVRRVIYRHTLPLRIMHWINAICL
                     LILLGSGLQIFNAHPALYWGQASHFDRPWLSLGARRATTGELRGVTQLGSHRFDTTGW
                     FGVSDVNGRTIVRGFPSWSTIPGSRWLSMGRAWHFFFAWLFAINGVLFVLYALWTRHL
                     TRDLVPTRRDWRGIGRSIIDHALLRHPRGEEAARYNVLQRLAYLVVIFFFGGGVVLMG
                     LAMSPRMDSVLGWLVDAVGGRQSARSIHFLFAMGFIAFFLIHIFEVIVTGVGNNLRSI
                     ITGRYAIETEQRDETHE"
     gene            4441..5193
                     /locus_tag="VFK00_10950"
     CDS             4441..5193
                     /locus_tag="VFK00_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET7593507.1"
                     /translation="MNRRRRFLRDALALTGAVVVAGCDRLSQTGWAPKVLGSATAVSH
                     AAQGALSRNAMAREYTQSDISPVFRPNGTIHPQTPAYRALAANGFRDYTLGVGGLVAH
                     PLSLSLEQLRELPTRTQITRHDCVEGWSVIGQWTGVQLSRVLQLARPTKAARYVVFRC
                     HDVMDDGNEYYESLGFDDAYHPQTLLAWELNGKPLPIPNGAPLRLRVPRQLGYKMAKY
                     LRRIDLVASLKGIEGGSGGYWEDQGYQWYAGI"
     gene            complement(5190..5954)
                     /locus_tag="VFK00_10955"
     CDS             complement(5190..5954)
                     /locus_tag="VFK00_10955"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7593508.1"
                     /translation="MLTWEHGRWLLRGAMVVIAVGAVIAFFASGAEHHLDYATLQQSR
                     EWLAAVQAAHPWRLAFAFLGILIGVVLLSLPLLTVMTLAAGAIFGVVEGTVLMSFGSA
                     IGATMVMLASRFIFKDEIRRRFSHRLHRVDAGIERDGAFYLLNIRLVPIFPFFLVNLL
                     MGLTHMRVRTFYWVTQLGMLAGTAVYVNAGTRIASAHSAGELVSIPMIASLALLAVLP
                     FASRWIIRRARHWRHERRWRHMGRSDEPGGNPGDES"
     gene            6095..7213
                     /locus_tag="VFK00_10960"
     CDS             6095..7213
                     /locus_tag="VFK00_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7593509.1"
                     /translation="MPETPPLLRRLRRDHLLQALLLLVVVLAIIDWRGPREYLRWLDL
                     PTLAGLAGLLVLTEGVRSSGYVQRFALHLIARLRTTRALAFVLLALSAVLATVLTNDV
                     ALFLVVPLTLAIDRVAGIPRQRLVVFEALAVNAGSTFSPIGNPQNLLLWQHSGLGFFG
                     YVAALAPTGAILLGVLLVACGFAFPAMPLQLEEDRIERALRLDTPLLAGSTLLLAAMV
                     AAMQWDFAIPAALAVIAIGLLFFRRVLAGVDWLLLATFAAMFVGLGHLAALPVVQSHV
                     GALDWHDARVTYTGGIVLSQIISNVPAAVLLQHYAPDLTLLAAAVNVGGAGLMIGSLA
                     NLIALRLDGSRGIGWRFHAWSIPYLVVTALLVAAARWG"
     gene            complement(7245..7997)
                     /locus_tag="VFK00_10965"
     CDS             complement(7245..7997)
                     /locus_tag="VFK00_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET7593510.1"
                     /translation="MDAPAALIAEDEPLLRAELRDALGSAWPELRICAEAADGDAAIR
                     ALQRYRPDILFLDIRMPGASGLEVAHVASGRSHVVFITAYDEFAVAAFEQGAVDYVMK
                     PFSTERLAIAVARLKQRLNQPPADLAEVIRSLLQRLDQGPERLRWITASRGRELHLVA
                     VEEILYFKADLKYTQMVTRDGEFLIRKTIRDLLAELDPDQFWQIHRSTLVNVTAIARV
                     QHGLDGHMRLRVGERPELLTVSRPFAHRFRQM"
     gene            complement(7976..9139)
                     /locus_tag="VFK00_10970"
     CDS             complement(7976..9139)
                     /locus_tag="VFK00_10970"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7593511.1"
                     /translation="MNVAARTLVATGAAAGILARSLQFVVESWRRITLKQVLVVNLIA
                     VLISVWDVLTWFRMSLAAAWQTLAWIFADNAMVAMGLLLVVAIADRVVPRRWPWWAPY
                     AVAALGGGVLVNLLASWCFQYVIPLATMTNQYVKPEYLHRMRTLTELADGVVTGGIAI
                     FVYAWLRRLRQQQDRLHEVQQAQVSARRRLSEARLQTMQARVEPELLVDTLGQIEALH
                     ATDATRALHTLDALIVYLRKAVPREHAHPPTLAGEIALVGAWLDVVRGAQGDVARLEV
                     SVPAAAGAATFPALVLPCLIRHEIAGMPRSPGSGSLLRIAADVAESRLRVHVVAVAAH
                     ASEHSDDRIESLRARLAALYGDRAHFLRRRCVQGDRCEVDTTIEIPQWMPPPP"
     gene            complement(9136..10269)
                     /locus_tag="VFK00_10975"
     CDS             complement(9136..10269)
                     /locus_tag="VFK00_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7593512.1"
                     /translation="MTANVSFDEARDVGGSPAATRRHGHGGIVRWGALVCLFAFCLAV
                     RPVASIYFEDMAEPLPKLLAMAWLVNLLVALPVLPIVALAERRTAGMTAWPRNAVLVA
                     AVTLATLLGVFLRAVFGSLVPGAGSIAKGGIEMVVGYWMDRTLLCSGLAAIYVFHARM
                     HELADAAHVEEITQQRLSRQGTEAALQSLHAQIEPHFLFNTLANVRRLLRSDPTAGRA
                     MLRHFQRHLRTSLTLIRARLIRVADAFELTEAYLQIQAVRMGARLRWRIQGDPAARTG
                     LLPPMMLITLVENAIKHGLGPLPDGGTLDISAHVANGRLELRVSDDGVGFRQSSGGGT
                     GLANVRNRLGLMYGEAAQLRLSRNTPRGVVATLSLPYQAGSPE"
     gene            complement(10417..13524)
                     /locus_tag="VFK00_10980"
     CDS             complement(10417..13524)
                     /locus_tag="VFK00_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7593513.1"
                     /translation="MANNTLWSAIQQALQTTGVPAALALGVVTMPAVAQDSGGNPPAP
                     PQTLETIVVTGSHVRRVDLETSNPIVAISSQQIQATGKLTLGDVVQQLPVITGGIQNP
                     SVNNGGGSGQTLVGLRGLGSSRTLVLVDGQRVLSGGATAAGGGIGAGVDLNTIPAAAV
                     ERIEVLTDGASAIYGSDAIGGVINIILKSNYQGAEFQANYGISDRGDGARRGASFTFG
                     QTSDKGSILAGVDYNKFDPVLQSQRKFSENAVSLSASNTYGGPGTAPAGLTYLAVPGS
                     GVSAIIGGSSFAARNKIIFTTAGPGTGSFAAPYNCGNGQLSLNPDAAASGKSPVTPAD
                     YHCFGSDPALSDKYNYASVNLILTPQERTNVFFKGVYHLADNLDFYGTAYHQKSTSAS
                     QLAPAVMGSGPNFDTGFRISKDNYYNPFGVDFAGPEGNTYTARLFPAGNRYFATGITY
                     DQLVAGFKGSVDIGQQNWQWDVGYNYGHMSQIVTNNGLPDQNILNLAAGPSFLNADGV
                     VQCGTAAAPIPLDDCTPFDPFNLFNANTQAVIRAAASPALINFWNQERTIHADINGGL
                     FDLPAGTVQLAAGASHRKEYTNNVVGNTLLLNPATGTCTLGSACSAHLQGGYNVKEAY
                     AEILVPLLKDMPFVNALNVTLGDRWSKYSTFGSTNNWKIGVEYRPIEDLLLRGTVSTV
                     FRAPTIGNVFGPPISSAPLLSADPCDHITVANPACANVPLDGSFVDRAVAAAQQLKAI
                     TSGSTYAGFPLGPESGKSFDFGAVYSPHFLPGLSASADIWRIYLDNVITVVGAQSVVN
                     LCFAGQTRFCPLITRAATPGNEGQIALLTLPTANLGRFDVKGVDFQANYKLPPFAFGQ
                     FSVGLSGTYLAQAKVQSAPGLPGNSVLNYVGIMGSTGSALQASCPGGAPGAICYFPRV
                     RGLGSLGWQWGPWDAQWTMNYISHFQVGSLDARQGATGAIGFAPGGPAGAYVLEYGNY
                     VYNNLQLGYSIQPINLRVDAGVDNVFDKQPPFLYANNSQNANTDPNDFDVLGRYYWAR
                     LTMKF"
     gene            complement(13824..14168)
                     /locus_tag="VFK00_10985"
     CDS             complement(13824..14168)
                     /locus_tag="VFK00_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593514.1"
                     /translation="MKTPIISFAIAIAIAGVAGTAVAGHQETLPPLQVSASEVWSCEP
                     PADAPNCGNFHRWLLANFSQREIGMLFGTPSSYPEYLSGGLKTLHRRYERKVLQEYLA
                     AQPAPGKPVAVR"
     gene            complement(14418..15572)
                     /locus_tag="VFK00_10990"
     CDS             complement(14418..15572)
                     /locus_tag="VFK00_10990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7593515.1"
                     /translation="MQPITCIEDLRKLYLKRVPRMFVDYCESGSWTEATLRRNLEDFA
                     RIQLHPRVAMGVEGGTTSATMLGQDVAMPVALAPVGMLGMQHADGELLTARAAAAFGV
                     PFTLSTMSIASIEDVAVFTKRPFWFQLYVMRDHAFIERLIDRAKAAGCSALVLTLDLT
                     VMGQRHRDIRNGLSAPPKPTPRNLANLARHPRWCLRMARTRRRGFGNIAGHVEGVSDV
                     SSLAVWTAEQFDRGLNWNDVEWIKRRWGGKLVMKGIMDPDDARLAVDAGADAIVVSNH
                     GGRQLDGAPSSISVLPEIAARVGSDCEVWLDSGVRSGQDVFKALALGARGVMLGRAYV
                     YGLGALGEAGVATALELIRRELDLTMVLCGVRSIAGIDRRALHEAARPSG"
     gene            complement(15787..>16172)
                     /locus_tag="VFK00_10995"
     CDS             complement(15787..>16172)
                     /locus_tag="VFK00_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HET7593516.1"
                     /translation="AAGGVVGILPDQQPKRGEGQFAPFFGLDANTMVLLPRIAQRTGA
                     TVLFAFAERLPRGAGFRIRILPAPDGIADTDLKVACTALNRGVEQCVELAFPQYQWTY
                     KRWAERPDPDTPDPYWLARNARRLG"
BASE COUNT         2695 a         5405 c         5388 g         2684 t
ORIGIN      
        1 gtgcgcagcg acacgccgag cgtgtccgcg agccgccgcg ccgggagcgc cgtgcggcgg
       61 ccctggagcg catggatgat caggaacagg cgatcggcgc ggcgcatccg cgcatcatca
      121 ccgatcaccc tccggacgac cagcggcgag agcctgttgc tgcttccgcg tatgccgtga
      181 aagcgcactc cgcaccagtt gcccgacgcc gaaccagccc cagtagatgc ggtggtgccg
      241 gatcatcccg ccggccaggt ccatcacttc caccagatcg acctggtcgc catcgggtgc
      301 cgcgcgggga tactcccacg tcaacctcga gccgtcgaaa aaataccggc cctcctcgcg
      361 atgccagcgc acgagctcgt tcggccgcct gcggccgccc tcctcgaaga aatgccgcag
      421 cgcggcgtgc ccctccaggg cgccgcgttc gacgccgagg atggcgggaa tcagcggcgt
      481 ctcgaggatc gcatcttccg cgtacagcga aaggagccct tccacgtcgc gttcgcgcgc
      541 atagcggtcc cacaagacgt agattcgctc accatcatcc cgaaattcca tcatgtacct
      601 ccgggtcccc gatctcagcc gagccccatc tccgccgcca gcactgcatc ggcctcgagc
      661 gcgcgttgct cggccggccg cgccatcacg cgctcgatgt agtcggcgat gaccggcgtc
      721 gcctcgacca tgccgaagcc tgtcacccac cgcaatgcat tgccccacag cacgtcggct
      781 gcgctgaagc gctcgccgag cagccacgga ccgggctcca gtgcctgcgt gatggcggcg
      841 acggtggtgt cgtaatcggc gtaaggcgat tgcgcgcgcg gcgccggctc gcgcttcatc
      901 gccttgtcgc cgatcgcggg ttcgaaacac gcggcgtaga aaaccatcca gcgcaggtac
      961 gaaccgcggt ccggatcgtc cagggccggt gccaggcctg cttcgcggta caggtccgcc
     1021 agatagatga aaatcgccac ggtttcggtc accaccacgc catcgtggcg gatggccggc
     1081 accttgccca tcggattgat ggcggcatat tccggcgaaa gctgctcgtg gcgccggaaa
     1141 tcgacgatgc atggctcgta cttggcgcca agctcctcca gcagcgcgcg ggtcatggcg
     1201 gcgcgactgt gcgggttgtg gaagaacacc aggtcggacg ggggaggcat gggcgtggct
     1261 ccatatcgat ggtcggctcg cacgataacc atccctgctg acaccgtgct gtcagcagcc
     1321 gctggcaagc ccgccacgaa gccgggacaa tggccggccc ggcacgagga acccatcatg
     1381 cagcgcgatc tccaagggca cgtttcgctg acccagcacc tgatcgagga gcagcggcgc
     1441 cacgacagcg ttaacgccga actccgcctc ctgatcgagg tggtcgcgcg cgcctgcaag
     1501 cgcatctcga tcgcggttgg caagggcgcg ctcggcggcg tgctgggcga cgccggcacc
     1561 ggcaacgtgc agggcgaggc gcaaaagaag ctcgacgtga tttccaacga gatcctgctc
     1621 gaggcgaacg cctggggcgg ccatctcgcg gcgtgcgcgt ccgaggaaat ggcggaaccc
     1681 tatccgattc cggatgccta tcccaagggc aactacctgt tgctgttcga tccgctggac
     1741 ggctccagca acatcgacgt ggacatttcg gtgggcacga tcttttcggt gctgcgctgt
     1801 ccggaaggtg tcacccatcc gggcgaaagg gatttcctgc agccggggac gcggcaggtc
     1861 gcggcgggct attgcgtgta cggcccgtcc acgctgctgg tgctgaccac cggacagggc
     1921 gtcaacgtgt tcaccctcga tcgtgaaatc ggcagcttcc ggctgacacg cgcgaacgcg
     1981 aggattccgg aggacacctc ggaattcgcg atcaacatgt ccaaccagcg cttctgggaa
     2041 gcgccgatgc agcgctacat tgccgatctc ctggccggca agacgggccc gcgcggacgc
     2101 gacttcaaca tgcgctgggt ggcctccatg gtcgccgacg tgcaccgcat cctgacccgc
     2161 ggcgggatct tcatctatcc gctcgacgag aaggtcaagg acaagggcgg caagctgcgc
     2221 ctgatgtacg aggccaaccc gatgagcttc atcgtcgagc aggccggcgg cgccgcgacc
     2281 accggccgct cacgcatcct cgacgtgcaa ccgacgcaac tgcatcagcg cgttccggtg
     2341 ttcctgggct cgaggaacga agtcgaaacc gcgacgaaat accacctcga agacgcgtag
     2401 gcggcgccca tttttgctgg tcaccccggc gcaggccggg atccggctct tgtgcggttc
     2461 gcagaaaggc cactggatgc cggcctgcgc cggcatgacg gcccgttgcg tatgcgggct
     2521 atacccgtcc cgtcaccggg atcagcgcgc cggtgatcgc cgaggcctgg tcggacagca
     2581 ggaacactat cacgtccgca agctgtgcgg gtttcaccca tttcgcgaaa tcggccttgg
     2641 gcatgtcgcg gcgattcggc ggggtgtcga tgatcgacgg caacaccgcg ttcacggtaa
     2701 tgccgcgcgg tttcaattcc tcggcgagcg attcggtgag gcgcagtaca ccggccttcg
     2761 aagccgtgta ggcacccacg ccggcaccgg ctttcagcgc cgacgccgcg ccgatgttga
     2821 cgatccgtcc gccctcggga agatgcgcca gcgcggcctt gctggcagcg accgcggtgc
     2881 gtacgttgat gcggtacagg aggtcccagg tgtcgggact gccgtcggcc agcgtctcga
     2941 aacggaaggt gccggcgatg ttgaccagtg catcgagccc gcctagcgcc ttggccgcgt
     3001 cgtcgatcgc ggctttcgcc tgcgcggcgt cggcgagatc cacgccgccg atggcgcggg
     3061 caccgtgcgt atcggcgggc ggatcggcgc ggtcgatcgc ggcaacctgc gcgcccgccg
     3121 cgagtgcggc ttggacaacc gcggctccaa gcgaaccgaa tgcgccggtg atcgcaatgc
     3181 gtttgcctgc gagtgacatg ggaacctcct gacggcgccg cgtcggaagc acggcacggg
     3241 atcgagcggc aagcatatcg ccgaccggcg gacgaggaca cgccgtccgc ttcatcgttg
     3301 ggcttcgccg cgctcagcgc caacctgcat ggccttcgta ggttgggttg atcccggact
     3361 tgtccgggag aagcctaacg gttttcgaac gtggctggcg aaacgggcac gacactcacg
     3421 tacgaattgg cagccttcgt aggctgggtt gatcccggac ttgtccggga gaagcccaac
     3481 ggctcgcggc atcgttacgg acggcaaacg acactcccaa ccgcgttggc agtacgctag
     3541 cgccgtcgca gcgcaaggaa ccggccatgg ccagctttcc cgaagacgcg attccaccgt
     3601 cgcccgccgc tgtgcgcccg gcccgcaccg tccggcgcgt catctatcgg cataccttgc
     3661 cgctccggat catgcactgg atcaatgcga tctgcctgct gatcctgctc ggcagcggcc
     3721 tgcagatttt caatgcgcac ccagcgcttt actgggggca ggcgtcgcat ttcgatcgtc
     3781 catggctgag cctcggcgcg aggcgcgcga ccaccggcga gctgcgcggg gtgacgcagc
     3841 ttggctcaca ccgcttcgac accaccggct ggttcggcgt ctcggacgtg aacggccgaa
     3901 ccatcgtgcg cgggtttccg tcctggtcga ccattcccgg ttcgcgctgg ctctcgatgg
     3961 gccgcgcctg gcatttcttc tttgcatggc tgttcgcgat caacggtgtg ctgttcgtgc
     4021 tgtatgcgtt gtggactcgg catctcaccc gcgacctcgt ccctacgcgg cgcgactggc
     4081 gcggcatcgg gcgctcgatc atcgaccatg cgctgcttcg gcacccgcgc ggcgaagaag
     4141 ccgcgcgcta caacgtgctg cagcgcctcg cctatctcgt cgtgatcttc tttttcggcg
     4201 gcggcgtggt gctgatgggg ctcgcgatgt cgccgcgcat ggactccgtg ctcggctggc
     4261 tggtggacgc ggtcggcggc cgacagtccg cgcgcagcat ccatttcctg ttcgcgatgg
     4321 gtttcattgc gttcttcctg atccacatct tcgaagtgat cgtgacgggc gtgggcaaca
     4381 acctgcgttc gatcatcacc ggccgctatg ccattgaaac ggagcagcgc gatgaaactc
     4441 atgaatagac gccgccgttt cctccgtgac gcgctggcgc tgaccggcgc agtcgtcgtg
     4501 gccggttgcg acaggttgtc gcaaaccggt tgggcgccca aagtgctggg cagcgcgacg
     4561 gcagtgagcc acgccgcgca aggtgcgctg tcgcgcaacg ccatggcgcg cgaatacacg
     4621 cagtccgaca tttcgccggt gttccgcccg aacggcacca tccatccgca gacgccggcg
     4681 tatcgcgcac tggcggccaa cggctttcgc gactacaccc tgggcgtcgg cggactggtc
     4741 gcgcatccgc tgtcgctgtc gctggagcag ttgcgcgagc tgcccacccg cacccagatc
     4801 acccgccacg attgcgtcga aggctggagc gtcatcggcc aatggaccgg cgtgcaactt
     4861 tcccgcgtgt tgcagttggc gcgcccgacg aaagcggcgc gttacgtggt gttccgctgc
     4921 catgacgtga tggacgacgg caacgaatat tacgaatcgc tgggtttcga cgacgcctac
     4981 cacccgcaga cgctgcttgc gtgggaactc aacggcaagc ccctgccgat tccgaatggc
     5041 gcacccctgc gcctgcgggt gccgcgccag ctcggttaca agatggcgaa gtacctgcgc
     5101 cgcatcgacc tggtcgcttc gctcaagggc atcgaaggcg gcagtggcgg ctactgggag
     5161 gaccagggtt accagtggta tgccggcatc taggattcgt cccccgggtt gcctccaggc
     5221 tcgtcactgc gccccatgtg ccgccagcgc cgctcgtggc gccagtgccg ggcgcgccgg
     5281 atgatccagc gcgacgcgaa cggcaggacc gcgagcaggg ccagcgacgc gatcatggga
     5341 atcgacacca gttcccccgc ggaatgcgct gacgcgatgc gggtgccggc gttcacgtac
     5401 acggcggtgc cggccagcat cccgagctgc gtcacccagt agaaggtgcg cacccgcatg
     5461 tgggtcagcc ccatcagcag gttgaccagg aaaaacggaa agatcggcac cagccggatg
     5521 ttcaggagat agaacgcgcc gtcccgctcg atgccggcat ccacgcggtg cagccggtgc
     5581 gagaagcgcc ggcgtatctc gtccttgaag atgaagcgcg aggcgagcat caccatcgtg
     5641 gcgccgatcg ccgaaccgaa cgacatcagc acggtgcctt cgaccacccc gaagatcgcg
     5701 cccgccgcca gcgtcatcac cgtcagcagg ggcagcgaaa gcaggaccac gccgatcagg
     5761 attccgagga atgcgaacgc gagccgccac ggatgcgccg cctgcacggc ggcgagccat
     5821 tcgcggcttt gctggagggt cgcgtaatcg agatggtgct cggcaccgga cgcaaagaac
     5881 gcgatcaccg cgcccacggc gatgaccacc atcgcgccgc gcagcagcca tcgaccgtgt
     5941 tcccatgtca gcatgctttc cagcatgcac gcgcgaaggt gaacgacacg tgccccatcc
     6001 ggaaggtgcg cgtccggccg ggcttcgcac cgccgccctt tgccgccgcc ccccgcgtgc
     6061 ggcagcatgg cggtcattga tcggaagaat cccgttgccc gaaacccctc cgctactgcg
     6121 ccgcctgcgg cgcgaccacc tgctgcaggc gctgctgttg ctggtcgtgg tcctggcgat
     6181 catcgactgg cgcgggccgc gtgaatacct gcgctggctc gacctgccga ccctcgccgg
     6241 cctggccgga ctgctggtcc tcaccgaggg cgtgcgctcg agcggctacg tgcagcgatt
     6301 cgcgttgcac ctgatcgcgc ggctgcgcac gacgcgcgcg ctggcattcg tgctgctcgc
     6361 gctgtccgcc gtgctggcaa ccgtgctcac caacgacgtg gcgctgttcc tggtggtgcc
     6421 gctgacactc gcgatcgacc gggtcgccgg gatcccgcgc cagcggctgg tggtcttcga
     6481 ggcgctggcg gtgaacgccg gctcgacctt cagcccgatc ggcaatccgc agaacctgtt
     6541 gctgtggcag cactcggggc tcgggttctt cggttacgtt gcggcgttgg cgccgaccgg
     6601 tgcaatcctc ctgggcgtgc tcctggtcgc ctgcggcttc gcattcccgg caatgccgtt
     6661 gcagctcgag gaggaccgca tcgagcgcgc cctgcgcctc gacacgccgt tgctcgcggg
     6721 ctcgacgctg ctgctggcgg cgatggtcgc ggcgatgcaa tgggatttcg cgattcccgc
     6781 ggcgctggcg gtgatcgcga tcgggctgct gtttttccgg cgcgtgctgg ccggcgtcga
     6841 ctggctgctg ctggcgacgt tcgccgcgat gttcgtgggg ctggggcatc tcgctgcgct
     6901 gcccgtggtg cagtcgcacg tgggcgcgct cgactggcac gacgctcgcg tcacctacac
     6961 cggcggcatc gtgctttcgc agatcatcag caacgtgccg gctgcggtac tgctgcaaca
     7021 ctacgcgccg gacctgacct tgctggcggc ggcggtgaat gtcggcggcg cggggttgat
     7081 gatcggttcg ctcgcaaacc tgatcgcgct ccggctcgat ggcagccgcg gcatcggctg
     7141 gcgtttccac gcgtggtcga ttccgtacct ggtggtcacc gcattgctcg tggcggcggc
     7201 gcgctggggg tgatcgggaa ccggttccgc cgccggcgac gcatctacat ctgccggaac
     7261 cggtgcgcga acggccggct gaccgtcagc agttccggac gctcgcccac gcgcagtcgc
     7321 atgtggccgt ccagtccgtg ctgcaccctc gcgatggcgg tgacattgac cagggtggag
     7381 cgatggattt gccagaactg gtcggggtcg agttccgcca gcaaatcccg gatggtcttg
     7441 cggatcagga attcgccatc gcgcgtgacc atctgcgtgt acttgagatc ggccttgaaa
     7501 tacaggattt cttccaccgc gaccaggtgc aactcccgcc cacgcgacgc cgtgatccag
     7561 cgcagccgct cggggccctg gtcgagccgt tgcagcaggc ttcgaatgac ctccgccaga
     7621 tccgccggcg gctggttcag ccgctgcttg agccgcgcca cggcgatcgc gaggcgctcg
     7681 gtcgagaacg gcttcatcac gtaatcgacg gcgccctgct cgaacgcggc gaccgcgaat
     7741 tcatcgtagg cggtgatgaa gaccacatgg ctgcgaccgc tggcgacgtg ggcgacttcg
     7801 aggccgctgg ccccgggcat ccggatgtcg aggaacagga tgtcgggccg gtaccgttgc
     7861 aatgcgcgga tcgcggcatc gccgtccgcg gcctcggcgc agatgcgaag ttccggccac
     7921 gccgaaccca gcgcgtcgcg cagctccgcg cgcagcagcg gctcgtcctc ggcgatcagg
     7981 gcggcggggg catccattgc ggaatctcga tcgtggtgtc gacctcgcag cgatcccctt
     8041 gcacgcatcg ccggcgcagg aaatgcgcgc gatcgccgta caaggcggcg agccgggcac
     8101 gcagggattc gatgcggtca tccgaatgtt cggatgcatg cgcggcaacg gcgacgacgt
     8161 ggacccgcag ccgggactcc gcgacatcgg ccgcgattcg cagcagggat ccggagccgg
     8221 gcgaacgcgg catgccggcg atttcgtgcc ggatcaggca cggcaggacc agggccggaa
     8281 acgtggcggc gccggcggcg gccggcacgc tgacttcaag gcgcgccacg tcgccttgtg
     8341 cgccccgcac gacgtccagc catgcgccga cgagtgcgat ttcgccggcc agcgtcggtg
     8401 gatgcgcgtg ttcgcgaggc accgccttgc gcaggtacac gatcaaggca tccagcgtgt
     8461 gcagcgcgcg cgtggcatcg gtggcgtgca gcgcttcgat ctgcccgagc gtgtcgacca
     8521 gcagttccgg ctcgacccgc gcctgcatgg tttgcaggcg ggcttcggac agccgccggc
     8581 gcgcgctgac ctgcgcctgc tgcacctcgt gcagccggtc ctgctgctgg cgcaggcggc
     8641 gcaaccatgc gtacacgaag atcgcgatcc cccccgtgac cacgccgtcg gccagctcgg
     8701 tgagcgtccg catgcgatgc aggtattccg gcttgacgta ctgattcgtc atcgtggcca
     8761 gtgggatcac gtactggaag caccaggaag ccagcaggtt caccagcact ccgccgccca
     8821 gtgcggcgac tgcgtacggc gcccaccacg gccagcggcg cggcaccacc cggtccgcga
     8881 tcgcgaccac cagaagcaac cccatggcga ccatcgcgtt gtcggcgaag atccaggcga
     8941 gggtctgcca ggccgccgcc aggctcatgc ggaaccaggt gagtacatcc cagaccgaga
     9001 tcagcaccgc gatcaggttg acgaccagaa cctgcttcaa ggtgatccgt cgccagctct
     9061 ccaccacgaa ctgcagcgac cgcgcgagga tgccggccgc ggcgcccgtc gcaaccagcg
     9121 tgcgcgctgc aacattcatt ccgggctccc tgcctggtac ggcaggctca aggtcgcgac
     9181 cacgccgcgc ggggtgttcc tcgagaggcg caactgtgcc gcttcgccgt acatcagtcc
     9241 caggcggtta cgcacgttgg caagcccggt gccgccaccg gaactctgcc ggaagccgac
     9301 accatcgtcg ctgacccgca gctccaggcg cccgttggcc acgtgggccg aaatgtccag
     9361 ggtcccgccg tcgggcagcg gccccaggcc gtgcttgatc gcgttctcga ccagcgtgat
     9421 cagcatcatc ggcggcaaca gcccggtcct ggcggcaggg tcgccctgga tgcgccagcg
     9481 caggcgcgca cccatccgca ctgcctggat ctgcaggtag gcctcggtaa gttcgaatgc
     9541 gtccgccacg cggatcaggc gtgcgcggat gagggtgagc gaggtgcgaa ggtgtcgctg
     9601 gaaatgccgc agcatcgccc ggcccgctgt cgggtcggat cgcagcaggc gcctgacgtt
     9661 cgccaacgtg ttgaacagga aatgcggttc gatctgggcg tgcagcgact gcagggcggc
     9721 ttcggtgccc tgtcgcgaaa ggcgctgttg cgtgatctcc tccacgtggg cggcatcggc
     9781 caattcgtgc atgcgcgcgt ggaacacgta gatcgccgcg agaccggagc acaaaagcgt
     9841 gcggtccatc cagtaaccga cgaccatctc gatgccgccc ttcgcgatgc tgcctgctcc
     9901 gggcaccagc gagccgaaca ccgcgcgcag gaaaaccccg agcagggtgg caagggtcac
     9961 cgccgcgaca agcaccgcat tgcgcggcca cgcagtcatc cccgcagtgc gtcgctcggc
    10021 cagggccacg atgggcagga ccggcagcgc caccagcaag ttgacgagcc acgccatggc
    10081 tagcaacttc ggcagcggct ccgccatgtc ctcgaagtag atgctcgcca ccggccgcac
    10141 cgcgaggcag aaagcgaaca ggcagacgag ggcaccccag cgcacgattc cgccatggcc
    10201 atgccggcgc gtggctgccg gggaaccccc cacgtcacgc gcttcatcga aggacacgtt
    10261 cgcggtcatg acttgccgtt caccctgcca cctggaatac gctcgaattc cacgccaggc
    10321 gaagcaactc gaagctactt gtagacctct ttacgcccgc cggcaagcat ggctcgtccc
    10381 cctcgcgcca agaggaatca tcccctcccg ggcccatcag aatttcatgg tcaggcgggc
    10441 ccagtagtag cggccaagta cgtcgaagtc gttcggatcg gtgttggcat tctgcgaatt
    10501 gttggcgtac aggaacggcg gctgcttgtc gaaaacattg tccacgccgg cgtctacgcg
    10561 gaggttgatc ggctggatgc tgtagcccaa ctgcaggttg ttgtagacat agttgccgta
    10621 ctccaatacg tatgcgccgg cgggaccgcc cggcgcgaaa ccgatggcgc ccgtcgcgcc
    10681 ctggcgcgcg tccagcgaac ccacctggaa gtggctgatg tagttcatcg tccactgcgc
    10741 gtcccacggc ccccattgcc aaccgaggct gccaagcccg cgaacacgcg gaaagtagca
    10801 gatggcaccc ggcgcaccac cgggacagga tgcctgcagc gccgaacccg tcgaacccat
    10861 gatgccgaca tagttcagca ccgaattgcc cggcaggcct ggcgcgctct gcaccttggc
    10921 ctgggccagg taggttccgc tgaggcccac gctgaactgg ccgaacgcga acggcggcag
    10981 cttgtaattg gcctggaaat ccacgccctt gacatcgaag cggcccaggt tcgcggtcgg
    11041 cagcgtgagc agcgcgattt ggccctcgtt gcccggcgtg gcggcgcggg tgatcaacgg
    11101 gcagaaccgg gtctggccgg cgaagcacag gttgacgacg ctctgtgcac cgaccaccgt
    11161 gatgacattg tccagataga tgcgccagat gtccgcgctc gccgacagcc cgggaaggaa
    11221 atgcggcgag tacaccgcgc cgaaatcgaa cgacttgccg gactcgggac ccagcggaaa
    11281 accggcgtag gtcgatccgg acgttatcgc cttgagctgc tgggcggccg cgacggcgcg
    11341 atccacgaag ctgccgtcca gtggcacgtt ggcgcaggcg gggttggcca cggtgatgtg
    11401 gtcgcaggga tcggcgctca gcagcggcgc gctgctgatc ggcggcccga acacattccc
    11461 gatcgtcggg gcgcggaaca ccgtcgagac cgtgccgcgc agcaacaggt cttcgatcgg
    11521 gcgatattcg acgccgatct tccagttgtt ggtgctgccg aaggtgctgt acttggacca
    11581 gcggtcgccc agggtcacgt tgagcgcgtt gacgaagggc atgtccttga gcaacggaac
    11641 caggatttcc gcgtaggctt ccttgacgtt gtagccgccc tgcaggtgcg ccgaacaggc
    11701 gctgcccaac gtgcaggtgc cggtggcggg attcagcagc agcgtgttgc cgacgacgtt
    11761 gttggtgtat tccttgcggt gggaagcacc cgcggccaat tgcaccgtcc cggcgggaag
    11821 gtcgaacagg cccccgttga tgtcggcgtg gatcgttcgt tcctggttcc agaaattgat
    11881 cagtgccgga ctcgccgctg cccggatgac ggcctgggta ttggcgttga agagattgaa
    11941 gggatcgaaa ggcgtgcaat catcgagcgg aatcggagcc gccgccgtcc cgcactgcac
    12001 caccccgtcc gcgttgagga acgaagggcc ggccgccagg ttcaggatgt tctggtccgg
    12061 gaggccattg ttcgtcacga tctgcgacat gtggccatag ttgtaaccga cgtcccactg
    12121 ccagttctgc tggccgatgt cgacgctgcc cttgaacccc gccaccaact ggtcgtaggt
    12181 gatgccggtg gcgaagtacc ggttcccggc cgggaacaac cgtgccgtgt aggtatttcc
    12241 ctcgggtccg gcgaaatcca cgccgaacgg gttgtagtag ttgtccttgg aaatcctgaa
    12301 gccggtgtcg aagttggggc cggaacccat cacggcggga gccaactggg aagccgacgt
    12361 ggacttctgg tggtacgcgg tgccgtagaa atcgaggttg tcggcgaggt ggtagacacc
    12421 cttgaagaac acattggtgc gctcctgcgg ggtcaggatc aggttgaccg aagcgtagtt
    12481 gtacttgtcg ctaagcgcgg ggtcgctgcc gaagcagtgg tagtcggcgg gcgtcaccgg
    12541 gctcttgccc gacgccgcgg cgtcgggatt cagggagagc tggccattgc cgcagttgta
    12601 gggcgcagcg aaggaacccg tgcccgggcc cgcggtggtg aagatgatct tgttgcgcgc
    12661 ggcgaacgag gaaccgccga tgatggcgga cacgccggag ccgggcaccg cgagataggt
    12721 caggcccgcg ggcgccgttc ccgggccccc gtaggtattg ctcgccgaaa ggctgacggc
    12781 gttttcggaa aacttgcgct gcgactgcaa cacgggatcg aacttgttgt agtcgacgcc
    12841 cgccaggatg ctgcccttgt cggaggtctg gccgaacgtg aagctcgcgc ccctgcgcgc
    12901 cccgtcgccg cgatcggaga tgccgtagtt cgcctggaac tccgctccct ggtagttgct
    12961 tttcaggatg atgttgatga cgccgccgat cgcgtccgat ccgtagatgg cggacgcgcc
    13021 gtcggtcagg acctcgatgc gctccaccgc ggcggccggg atggtgttca gatcgacccc
    13081 ggcaccgatt ccgccaccgg ccgcggttgc accgccgctg aggacgcgct gcccatccac
    13141 cagcacgagt gtgcggctgg aacccagtcc gcggaggccg accagggtct gtccggaacc
    13201 gccgccgttg ttgacgctcg gattctggat gccgcccgtg atcaccggta gctgctgcac
    13261 gacatcgccg agcgtcagct tgccggtcgc ctggatctgc tgcgacgaga tggccacgat
    13321 cggattggac gtttccaggt ccacgcgccg cacgtgcgaa ccggtgacca cgatggtttc
    13381 gagggtttgc ggcggcgccg gtggattccc gccgctgtcc tgggccaccg cgggcatcgt
    13441 gacgacgccc agggccagtg cggccggcac gccggtcgtc tgcaatgcct gctggatcgc
    13501 actccacaac gtgttgttcg ccatgcgagt ctccggccgt tgcgcgcttc gggaaagatc
    13561 ttcatccggc attgccgact ggcttcatgg tgcgaagttg cgaccggact ggtaagcggg
    13621 caggcgacga aacgacgcat cggtgatgcg aaatgccgga atggcggcgc caaccgtcgc
    13681 atcgacaagc gacgtagctt cgattcgcgc gtgatcggcc agcgagcgtc cgcgcggttc
    13741 tgcgaacgct gctgcatgcg gatcggccgc ggacaacaaa cgccccgcgg atgcggggcg
    13801 ttcgctacgt gccgccggga ggctcatctg acggcaacgg gtttgcccgg tgcgggctgg
    13861 gcggcgaggt attcctgcaa caccttcctc tcataccgcc tgtgcagcgt cttgagtccg
    13921 ccgctcaggt actccggata ggacgagggc gtgccgaaca gcatgccgat ttcgcgctgc
    13981 gagaaattgg cgagaagcca gcggtggaag ttcccgcagt tgggcgcgtc ggccgggggc
    14041 tcgcaactcc agacctccga cgcggaaacc tgcagcggcg gcaaggtctc ttgatggccg
    14101 gccacggcgg tgccggcgac cccggcgatt gcgatggcga ttgcaaagct gatgatgggc
    14161 gttttcatga cggggtcctc gatgattgca gccggccctg ggtgggcggc ctgcacgccg
    14221 ggcgtgatgc ccgggtaccg gcacgagcgc gcggatggaa agctcgggga tcggctcgat
    14281 cgtgccgtcg ggaaaagtag tcctggccat ggacggcgat aacccggcac gcgacgaaac
    14341 gaccgacgaa cggcctgaat cgccggattc cagcgtcgaa aatggccggc gaaccttccg
    14401 ccgcggtggg tgccggctca gccggacggc cgcgcggctt cgtgcagcgc gcgccggtcg
    14461 atcccggcga tcgaacgcac gccgcacaag accatcgtca aatccagttc gcggcggatc
    14521 agttccaggg ccgtggcaac gcctgcctca cccaacgcgc ccaggccgta cacgtacgcg
    14581 cgcccgagca tcacgccgcg cgcgccgagc gccagggcct tgaacacgtc ctgtccggag
    14641 cgcacgccgc tgtcgagcca cacctcgcaa tcgctgccca cgcgcgccgc gatctccgga
    14701 agcacgctga tcgacgacgg cgcgccatcg agctggcggc cgccgtggtt ggagacgacg
    14761 atcgcgtcgg cgccggcatc cactgccagt cgcgcatcat cgggatccat gatgcccttc
    14821 atcaccagct tgccacccca gcgccgcttg atccactcca cgtcgttcca gttcagcccg
    14881 cgatcgaact gctcggcggt ccacacggcc agcgaggaca cgtcgctgac gccttcgacg
    14941 tggccggcga tgttgccgaa cccgcgccgc cgggtgcgcg ccatccgcag gcaccagcgc
    15001 ggatgccgcg caagattggc gagattccgc ggcgtcggct tgggcggtgc cgaaaggccg
    15061 ttgcggatat cgcggtggcg ctgacccatc accgtcaggt ccagcgtcaa taccagcgcc
    15121 gagcagcccg cggcctttgc gcggtcgatg agccgctcga tgaaggcatg gtcgcgcatc
    15181 acgtacaact ggaaccagaa cggccgcttc gtgaacacgg ccacgtcctc gatcgatgcg
    15241 atgctcatgg tcgaaagcgt gaaaggaaca ccgaacgccg ccgccgcgcg ggccgtcagc
    15301 aattcaccgt cggcgtgctg catcccgagc atacccaccg gcgccagcgc gaccggcatg
    15361 gcgacgtcct ggcccagcat cgtcgcgctg gtggtgccgc cctcgacccc catcgcgaca
    15421 cgtgggtgca attggatgcg cgcgaagtcc tcgaggttgc ggcgcagcgt cgcttcggtc
    15481 caggaacccg attcgcagta gtccacgaac atgcgcggca cgcgcttcag gtagagcttg
    15541 cgcaagtctt cgatgcaggt gatcggttgc atgggcactc ccgaaacagg cacgacgttg
    15601 ggcttctccc ggattgcatc cgggatcaac ccaacctacc ccgcgcttcg atgcaggtga
    15661 tcggttgcat ggcgcgctcc cagaaatgta ggttgggttg acccggcgct ttttgccggg
    15721 gaagcccaac attcctgcgc aacgtacaac gtcgaatgtt gggcttccgc cggatcagat
    15781 ccggcgtcaa cccaacctac gcgcgttcct ggccagccaa tacggatccg gcgtatcggg
    15841 atccggccgc tccgcccagc gcttgtaggt ccactggtac tgcggaaagg cgagttcgac
    15901 gcattgttcg acgccgcggt tgagcgcggt gcaggcgacc ttcaaatcgg tgtcggcaat
    15961 gccatcgggc gccggcagga tgcggatgcg gaatcccgcg ccccgcggca accgctcggc
    16021 gaacgcgaac aggacggtgg cgccggtacg ctgtgcgatc cggggcaaca acaccatggt
    16081 gttggcatcg agcccgaaga acggcgcgaa ctggccttcg ccgcgcttgg gttgctggtc
    16141 cggcaggatc ccgaccacgc cgcctgctgc ca
//
LOCUS       DASSNH010000297        16159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1181444, whole genome shotgun sequence.
ACCESSION   DASSNH010000297 DASSNH010000000
VERSION     DASSNH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 16159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16159
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181444"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4551
                     /locus_tag="VFK00_11000"
     CDS             <1..4551
                     /locus_tag="VFK00_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7593517.1"
                     /translation="HIGPLSSFVLWSAMVDALLAELSGGKYEPSELQLLFAGGIHDAV
                     SSALVQVLAAPLAARGVRVGILMGSAYLFTREIVENGSIVPQFQQEVLDCRKTIALES
                     GPGHASRCAYTPFAEEYFRKRAEMRERKVPGDEARQVLDQLILGRLRIASKGRMRDGL
                     NGDLRTLEVAQQRADGMYMLGQVATLRHARTDIRALHADVTTSAAEVLAAASVRAAEA
                     PAAAGNGNGRAPADVAIIGMACFLPKAPSVRKYWANILDRVNAITEIPLDRWDSRLYF
                     DADRQAKDKIYSKWGGFLDDMLFDPTRYGMPPKSVESVDPMQLMALEVAQRALTDAGY
                     AEKPFDRERASVIVGASGGAGDVGIQYGLRSELPRFTGNLPDSVAERLPTWTEDSFAG
                     ILLNVVAGRIANRLNFGGINCIVDAACASALAAVYQGVSELTGGRSDLVLAGGVDTVQ
                     GPFGYLCFSKTQALSPRGRCATFAADGDGIVISEGIAMIVLKRLADAERDGDKIYAVI
                     KGVGASSDGNAKGLTAPLPAGQLRAMRRAYAQAGFGPRAVGLFEAHGTGTVAGDTAEL
                     ESTMQLVKADGGTRHQSVIGSVKTMIGHTKATAGVAGLIKASLALHHRVLPPQLTEAL
                     PNPVLRADDSPLYLVDEARPWLASPEAAPRRAAVSAFGFGGTDFHVVLEEYGDEYRDW
                     LRSAPLCDWPLELLVWSAPDRAALKQKLAETRGQLAELAGVELRHLAASLARHWQPEA
                     EVLTVAAQDIPSLVEQLDAAIDWLDGRRKALPPKVSHGTTAGERGKVALLFPGQGSQY
                     TGMLRQAALQFPVCAESLSAADRALEPAFSMRHGEGVRLSDFIFPRGAYDDAQGESAT
                     QALTSTDIAQPALGAVEVALLRLMQGFGLEADMVAGHSYGEFVALHAAGAIDFETLLR
                     LSARRGECIVDAVREAGSELGTMAAVRAPREEVARVIGGIDDVVVANHNAPEQVVISG
                     SDRGVEEATRALTKAGIAATTLPVAAAFHSRHVRPALAAFTSAIDAMDWATPQLPVYA
                     NASGKPHGGDVAALKRAMAEHLVQPVEFVAQIEAMYADGARVFVEVGPKAVLTQLTRR
                     ILGDRPHAAIAIDRGNGLADLLAAFGQLLCAGIELDVRALFKRRECRIADPSHLASLG
                     TAPSISRTAWLLNGSSARRASESPRQIGVTVEQTGSPVKSSTRSGPPVEPGSAGKLHA
                     ITPTQGPHPEAPTYAPDTLIAQRPAWGVHGDGTYRSDAQEFHMNEQRPSMNLDAAVMT
                     EYFATMRQFLDTQSHVMTMVMGGSLAPRGVAATPRMIAPPITVPRPAEAVIRPNHAAS
                     PYINGSGHAPAVASNGNGHAPAMASNGHAGGMAPNGNGHAAGMASNGNGHAPAMATHG
                     NGHAPPVTLQATPATPATVPSKAREEVVQVAHGNGAAGSPAAAGSIVDRDKLGDILLG
                     IVEEKTGYPRDMVGLDQNLEADLGIDSIKRVEVVGAMLQSLPATQREALTSRRSELNK
                     QATLKGILDLLATSVDRAAAAA"
     gene            4686..5603
                     /locus_tag="VFK00_11005"
     CDS             4686..5603
                     /locus_tag="VFK00_11005"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7593518.1"
                     /translation="MAAPVFLLAPPRSYTSLVNAMIGQHPQAFGLPELCLFNVDRLMG
                     LWDDTEGESPERLAMWRQGLMRAIAEIYCGEQSTATINMAYLWASVREDWETGRVYRE
                     LVDRLDPLVAVEKSPSYTTTVPRMQAMYRTFPDARFIHLVRHPIGQGKSLTKIDEDNA
                     FLTLMGAFEIHEDHATFDPQIAWHDINLNILEFLESVPDEQQIRVRGEDIMADPHTRL
                     AEIARWLGWRDDPEAIEEMMHPERSPFACIGPWTAMFGNDSNFLSGPTFRPHKPKIPK
                     ADAPLPWRTDGQGLYPEVVSLMREFGYDD"
     gene            5593..6048
                     /locus_tag="VFK00_11010"
     CDS             5593..6048
                     /locus_tag="VFK00_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593519.1"
                     /translation="MTTDTETDGSAAPEQKALPAPQSSKRQRLSPVPVNERGRVDPSQ
                     MNPYQAALRQSLVNSVDARKARRLVQRQIGHIKSSRDGAAAIKFLFDEHGRAPANAPL
                     EDIVEERRRTEYHLLWLEGFVAEMRNRLVKIRRVEDAALEVLAQAHPDE"
     gene            6041..7654
                     /locus_tag="VFK00_11015"
     CDS             6041..7654
                     /locus_tag="VFK00_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7593520.1"
                     /translation="MNEIQSRGASGNRHVLGRAAGRVLVAGMVAVALGGCVVGPAYHR
                     PDIPLPQAWRVPAGTADAVVNTAWWKGFQDPDLDALIKQAIDANMDLLQATARVRQYE
                     AKLDVASGARYPEATYDIGAERRHYSTAHPTALGILNEPTQNDFGVGIGLNWELDIWG
                     KLKRADQAALADLLSSQYARHAVMLTVVTNVATSYFQLLALDDQLRLAQQTLRNRGAA
                     LNLIENRYKGGSSSKMAVLQARSAVDEVWVAIPDLERQIAVLEDAMSILVGRNPGKII
                     RHRDDEPALPPVPGGVPSDVLSRRPDVLAAEQQLVGANARIGVAKAALFPSISLTGMF
                     GVASNALDTLTDGDATEASIGLGLLGPIFAGGRLKAGIREAEAYQQELLVKYRQTVLT
                     ALGNVDDALTFNVKANEIARRGEQQVATLNDRLHLSEVRYNGGQADYLEVLTAERDLY
                     NAQAQQVDRHRDTYLAMVSIYGAMGGGWMVEQDKLRAADKQDTASAAQSKPTTTNPGG
                     KGAPAAAAPAAAMPASAPPTKVKSPESAQ"
     gene            7651..10110
                     /locus_tag="VFK00_11020"
     CDS             7651..10110
                     /locus_tag="VFK00_11020"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7593521.1"
                     /translation="MSETSRKVALPAYLVRRCEAFRVVSGAEHSYVVRDKVQNKTWDF
                     DPWQFFILEVLPGCESVEKLQTAFRDRFNRIVTNAELEEFFATVADHRLLNESALQHP
                     LLSVFARRTYDVVDGKPVARPHSSLFAHKDSPAAPSMDSEVAEAVAEAQPEEEVLAAG
                     VQDVPGSDRRTADKMIDLFDPRPILRFLAPALAPLRYVGYAFPVMLLAAVLVLIQHWQ
                     SFATDVGGIRIAVGLGGHLLFALLTVNLASVIVRACIAHNYGAQVESFGIMFFAGFAP
                     RFDVGIRHLGKLSREQRMWYHGGNLLIRGVLLCVGVLAWFFTIDKPGSAANDAGLLLA
                     LTSWTGLVIETGNPFIRGSTYFLLATYMDEPKLRVKARRALMSKIHRKVYQQADNNLL
                     AAYGLACIAYTTLLIIFLTLAVGNWLSNRLGIGIGVYLAAAALCGYVVWRTYKQLKKV
                     GAIYQRSQEFDRWRSRTLVTEDAAEGEPEVPKKHYWRYVALLCLLLLLLIPYQYDVTG
                     SVEVYPYRKQQISTDTPGLIDQVNFDGGESVKKGTVIATLAHEDYNSQIKVTEAEIAA
                     QAAVVANLKSLPKPQEIELAQEALRVAQTQASFSVAKVPRLQALYNQGAVPLEDLETA
                     RKEAHTDVQQVQEKQAALALAKTGPTADQIAAAEAKLASLQAQKALYEDKLARTTLRM
                     PFDGNILTLHLKDRINSYLNQGDPFAEVEYTGKVTAQIDIDESDLQYVKVGSTVRLRP
                     TAYFNREFDGVVTKIGQSVTSTPTGTYAAVIATFQNEDGSLKTGMTGEAKIDGPTIPV
                     WRAFTQSIQRFVQVEVWSWVP"
     gene            10167..12998
                     /locus_tag="VFK00_11025"
     CDS             10167..12998
                     /locus_tag="VFK00_11025"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7593522.1"
                     /translation="MNQYTRHDRQCDPVVLTDYFATMRHFLETQGRMMSMWAGHAGHA
                     GRAAEIGSAGLADARRVPAKVNPVRPGRPAAVRGPAMAPAAVRPPKRAGTSVAGARAV
                     MRAQAEPIPPGAARRLSRGRFVLTRDRLGVADRLAAALRARGAEVVAIEPGVLANEDA
                     LLQRCEALRAEAPIAGIVHLAALGMPAINGEESPSTWRQMLQENEKALFLLLRGLSPK
                     LIDDAHVVVASDLGGLFGREGSSDGALRLGAGGIGTVKSLRAERESLRVKAIDLDPRR
                     EAAALAADLLGEIELDGGRQEVGYPDGQRTVFRTTPEPVPTDPATVAASRGLVVLATG
                     GARGITAETLRELARPGNTLVLVGRSPLAGEPGELAALAGEAALREHFVAQVRAGKAK
                     WNPREIGKRVAAVLAQREMRANLDDLRERGASVEYVAADVTDEDSIRALVAEVTQRHG
                     PISGVVHGAGVIEDKLLAEKSSDSWSRVVETKVIGLLLLQKYVDSRALKFFTVFSSVA
                     GRYGNSGQSDYACANELMNRLCVALQARWGDNVAVSALCWGPWGATRFGAGMVTADTE
                     AKFARRGVKLVSAELGRALFRDEITRAPGAPVEIVCGEAPWDRREAELGVIGTGSAEA
                     SSVDAAKAAVDTAAAAGTEPLLGDGVWDAQPTGERSVSVELDRARHLYLDEHMLDGKQ
                     VLPAAAALEMMAEAGRSLWPGWQVAEVREHKLMKGVEMQSRPRRLAVLVYPPTYGSSE
                     GFEVTAELKGELAPGKMVTHYRAVLHLAQSLPDPVPVERGRHTEKALAVAKAYGEWLF
                     HGPRFQVIESIDGLSRTGAGARVRVSRPGEWLAGCAAEGPAWVFDPALLDAAAQMAWL
                     WSRAFRDEAALPTRFGRVTRHRASCPERMHMEYSRTDTEDPSLIRGNVTFLDEHGEPV
                     LAIEELDSIASAALNRFGGTATAGQKVTS"
     gene            12995..>16159
                     /locus_tag="VFK00_11030"
     CDS             12995..>16159
                     /locus_tag="VFK00_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET7593523.1"
                     /translation="MNRTVSQRPAPAPIAIIGMACIFPQAPDLAAFWNNILAGVDAID
                     DPVPEWGAERYLAAGRIRTARGGFLKDLYRFDPREFGIMPNSVDGGESDQYLALRVAR
                     DALADAGYLGADADHHATGIVLGHSAYLHRGQVTVIQNNIVLDQTMDLLRVALPHLGD
                     AGLDEIREILRQKLPPTTADNAPGLVPNMMTGRIANRLNLRGPNYVLDAACASSLLAV
                     AAAADELRNGRSDMMLAGGVNATLPADVTTSFTQLGALSARGRVRPFEAGGDGTLLGE
                     GLGVVVLKRLDDAIAQGDRVYAVLRGVGRSSDGRGTGLLAPSQDGETLAMQRAYRQTG
                     IDPATIDLVEAHGTGIPLGDRTEIASLKTVFGDRGSEFGTKALGSVKSMISHCIPAAG
                     IAGLIKVSMALHHRVLPPTLCDEVNPELGIEQTPFYVNTRCAPWMARHGAPRRAGVNS
                     FGFGGINAHAILEEAPPEARAPGRFTSWPAELCVLSAASAAALVEKLERLVAALSANP
                     QWSIAQVAAALAREDRAEEHRVAILARDTTELTTSIALAIGKVGEGASTWKASRARQV
                     YGRGHSDARLGFIFPGEGSQYPGMFADIALFPQVQHWLDFWHGMHDLPAGQTHTDIVF
                     PTCEVRHEHLTGREQRLHDMDVGSEAAFIGGMAMFDLLRALGVEPDVMVGHSSGESAA
                     LAASGASPAHSDLELVACISRQYAVYEALLKSDKIPTGALLAVGALPGEQIQAAVDSH
                     GGKVQVAMDNCSNQQVLYGSVEAIGQIEDELVRQGGICIRLPFDRGYHTPAFEDASRA
                     FLAYYEDIKVGRPKVPLYSCATAALFPKTPASVRKLAAQQWSRKVRFPETVRKMYDDG
                     VRIFLEVGPSGNLVSFVNDILAGRDFLALPTNVRGRNGIEQVLSVLARLYALGRWNAL
                     DRVYAGREITPIELGDRSPAARPPLLANTMPMVRLGPADCERIGALAAVARTPGPTAS
                     ADGSGLPGAEVGGGEDAAASTGLPPGGEGHAHSTHANDSATPLLDVVLELDENHIVAL
                     CRVSLEHDQFIRDHVISGPVSVDPA"
BASE COUNT         2755 a         5302 c         5556 g         2546 t
ORIGIN      
        1 cacatcggtc cgctgagcag cttcgtgctg tggagcgcga tggtcgatgc gctgctggcg
       61 gagctgtccg gcggcaagta cgagccgtcc gaactccagt tgttgttcgc gggcggcatc
      121 cacgatgcgg tgtcgtcggc attggtgcag gtactggctg cgccgcttgc cgcgcggggt
      181 gtgcgcgtcg gcatcctcat gggaagcgcc tatcttttca cccgtgagat cgtcgagaac
      241 ggctccatcg tcccgcaatt ccagcaggaa gtactcgact gccggaagac gatcgcgctg
      301 gaatccgggc ccggtcatgc aagccgctgc gcctacacgc cgttcgccga ggagtacttc
      361 cgcaagcgcg cggagatgcg cgagcgcaag gtgcccggcg acgaggcgcg ccaggtgctg
      421 gaccagttga tactcgggcg cctccgcatt gcgtccaagg gtcgcatgcg cgacggcttg
      481 aacggagacc tgcggacgct cgaggtcgcg caacagcggg ccgacggcat gtacatgctc
      541 ggacaggtcg cgaccctgcg gcatgcccgg accgacatca gggctctgca cgccgatgtg
      601 accaccagcg cagccgaggt gctggccgcg gccagtgtcc gcgcggcgga agcaccggcc
      661 gcggccggga atggcaacgg ccgcgcaccg gccgacgtcg cgatcatcgg gatggcctgc
      721 ttcctgccga aggcaccttc ggtccggaaa tactgggcca acatcctcga ccgcgtgaac
      781 gcgatcaccg agattccgct ggatcgatgg gattcgcggc tgtatttcga cgccgaccgc
      841 caggccaagg acaagatcta ttcgaagtgg ggcggcttcc tggacgacat gctgttcgat
      901 cccacccgct atggcatgcc acccaaatcg gtggagtcgg tcgaccccat gcagttgatg
      961 gcgctggaag tcgcacagcg cgcattgacg gacgcgggct atgccgaaaa gccgttcgat
     1021 cgcgagcgcg cctcggtgat cgtgggcgcc agcggcggcg cgggcgacgt cggcatccag
     1081 tacgggttgc gctccgagct gccgcgcttt accggaaacc tgcccgacag cgttgccgaa
     1141 cggttgccga cctggaccga ggacagtttc gccgggatcc tgctgaacgt ggtcgcgggg
     1201 cggattgcca accggttgaa cttcggcggc atcaattgca ttgtcgatgc ggcttgcgcg
     1261 tctgcgctcg cggccgtgta ccagggtgtt tcggaattga ccggggggcg cagcgacctg
     1321 gtgctggccg gcggcgtgga caccgtgcag gggccgttcg gctacctgtg cttcagcaag
     1381 acacaggcgc tgtcgccgcg tggacggtgc gcgaccttcg ccgccgatgg cgacggcatc
     1441 gtgatctcgg aagggatcgc gatgatcgtc ttgaagcggc ttgcggatgc cgaacgcgac
     1501 ggcgacaaga tctacgccgt gatcaagggg gttggcgcca gcagcgacgg caacgccaaa
     1561 ggcctgactg cgccgctgcc ggccggccag ctgcgcgcga tgcgccgcgc gtacgcgcag
     1621 gcgggattcg ggccgcgcgc ggtgggcttg ttcgaggcgc acggcaccgg cacggtcgcc
     1681 ggcgacactg cggaactgga aagcaccatg caactggtca aggccgatgg cggcacgcgc
     1741 catcagtcgg tgatcggttc ggtgaagacc atgatcggcc acaccaaggc cacggccggc
     1801 gtcgcgggtt tgatcaaggc ttcgctggcg ttgcaccacc gcgtgttgcc gccgcaattg
     1861 accgaagcac tgccgaaccc ggtcctgcgc gcggacgaca gccccttgta cctggtcgac
     1921 gaggcgcggc cctggctggc ctcgccggag gccgcgccgc gccgcgctgc cgtcagcgcg
     1981 ttcggcttcg gcggcaccga cttccacgtg gtgctcgagg aatacggcga cgagtatcgc
     2041 gactggctgc gctccgcgcc gctgtgcgac tggccgctcg agttgctggt gtggagcgcg
     2101 ccggaccgcg ctgcattgaa gcagaagctt gccgaaacac ggggccagct cgcggagctc
     2161 gcgggcgtgg aactgcgcca tctcgcggcg agcctcgcgc ggcattggca gcccgaggcg
     2221 gaggtgctaa ccgttgccgc gcaggacatc ccctcgctcg tcgaacagct cgacgccgcg
     2281 atcgactggc tcgacggcag gcgcaaggcg ctgccgccga aggtgtcgca cggcacgacg
     2341 gcaggcgaac gcggcaaggt cgcgctgctg ttcccggggc agggttcgca atacaccggg
     2401 atgttgcgcc aggcggcgct gcagtttccc gtgtgcgcgg aatcgttgtc ggcggccgac
     2461 cgtgcgctgg aaccggcctt ctctatgcgc cacggcgagg gcgtgcgcct cagcgacttc
     2521 atcttcccgc gcggtgccta cgacgacgcc cagggcgaaa gcgccaccca ggcgttgacc
     2581 agtaccgaca tcgcccagcc ggcgctcggc gcggtcgagg tcgcactgtt gcgattgatg
     2641 cagggattcg ggctcgaggc cgacatggtc gccggccaca gctacggtga gttcgttgca
     2701 ctgcatgccg ccggtgccat cgatttcgag acgttgctgc ggctgtcggc gcgacgcggc
     2761 gagtgcatcg tcgatgcagt gcgcgaggcc ggcagcgagc tgggcaccat ggccgccgtg
     2821 cgtgcccccc gcgaggaggt ggcgcgcgtc atcggcggca tcgacgacgt cgtcgtcgcc
     2881 aaccacaatg cgcccgaaca ggtggtcatt tcgggctcgg atcgcggcgt ggaggaggcg
     2941 acccgcgcac tgacgaaagc cgggatcgcg gccaccaccc tgccggtggc cgcggcattc
     3001 cactcgcggc acgtccgtcc cgcgctggcc gcgttcacgt cggccatcga tgcgatggat
     3061 tgggctacgc cgcagctacc ggtttacgcc aacgccagtg gcaaaccgca tggtggggac
     3121 gttgccgcac tcaagcgcgc catggcggaa cacctggtgc agccggtgga gttcgtcgcc
     3181 cagatcgagg cgatgtacgc cgacggtgcg cgcgtgttcg tggaagtcgg cccgaaggcg
     3241 gtgctgaccc agttgacccg acgcatcctc ggcgaccggc cccacgccgc catcgcgatc
     3301 gaccggggca atggtttggc ggacctgctc gcggcgttcg gccagttgct gtgcgcgggc
     3361 atcgaactcg acgttcgcgc actgttcaag cgccgcgaat gccgcattgc ggatccatcg
     3421 catctggctt cgcttggaac cgcgccatcg atttcgcgta cggcgtggct gctcaacggc
     3481 agcagcgcgc ggcgggccag cgaatctccg cgccagatcg gcgtgaccgt cgagcaaacc
     3541 ggctcgccgg tgaaaagttc gacccggtcg ggtccgccgg tggaaccggg cagcgccggc
     3601 aagttgcatg cgatcacccc cacgcaaggg ccacatcccg aagcaccaac ttatgctccc
     3661 gacaccttga tcgcccagcg accggcatgg ggcgtgcatg gcgacgggac gtaccgaagt
     3721 gacgcacagg agttccacat gaacgagcag aggccttcga tgaacctcga tgctgctgtg
     3781 atgacggaat acttcgccac gatgcgccaa ttcctcgaca cgcagagcca cgtgatgacc
     3841 atggtcatgg gcggcagcct tgcgccgcgc ggggtcgcag ccacgccccg catgatcgca
     3901 ccacccataa cggtgccgcg accggccgag gccgtgatcc gcccgaacca tgcggcgtcg
     3961 ccgtacatca acggcagcgg tcatgcgcca gccgtggcat ccaacggcaa cggtcatgcg
     4021 ccggcgatgg cgtccaacgg tcacgccggg ggcatggcac ccaatggcaa tggtcacgcg
     4081 gccggcatgg catcgaatgg caacggccat gcgccagcca tggcgaccca tggcaacggc
     4141 cacgcgccgc ccgtcacgct ccaggccacg ccggcaaccc cggcaacggt tccttccaag
     4201 gccagggagg aagttgtgca ggtcgcacac ggcaatggag ccgcggggag ccccgcagcg
     4261 gctggttcca tcgtcgatcg cgacaagctc ggcgacatcc tgctcggcat cgtcgaggag
     4321 aagaccggct atccgcggga catggtgggg ttggaccaga acctcgaggc tgacctgggg
     4381 atcgattcga tcaagcgcgt cgaggtggtt ggtgccatgc tgcagtcgct gccggcgacc
     4441 cagcgggaag cattgacgtc ccggcgcagc gaactcaaca aacaggcaac gttgaaaggc
     4501 atcctggatc tgttggccac gagcgtcgac agggctgccg ctgctgcctg agggggcatc
     4561 acatcgtgtt gctgcgttcc cgccccgccc gagaggcaag gggctgcgtc ttcaccaccc
     4621 ccgccgaacg gctgccgccg ggtttgtacg cgcggcaacc cggtgctggc tgacggagcc
     4681 ctggcatggc tgcgcccgta ttcctgctcg cgccaccacg atcctatacg tcgctcgtca
     4741 acgcgatgat cgggcagcat ccgcaggcat tcggcctgcc ggaactgtgc ctgttcaacg
     4801 tcgaccggct gatgggcttg tgggacgaca ccgagggcga gagccccgag cgcctggcca
     4861 tgtggcggca gggcctgatg cgggccatcg cggaaatcta ttgcggcgag cagtcgacgg
     4921 ccacgatcaa catggcctac ctctgggcat cggtgcgcga agactgggag accggacggg
     4981 tgtaccgcga gttggtggac cggctggatc cgctcgtggc ggtggagaaa agcccttcct
     5041 acacgacgac cgtgccgcgc atgcaggcca tgtaccggac tttcccggat gcccgcttca
     5101 tccatctggt gcgccatccc atcggacagg gaaaatcgct gaccaagatc gacgaagaca
     5161 atgcgttcct gacgctcatg ggcgcgttcg aaatccacga ggaccatgcg acgttcgatc
     5221 cgcagatcgc gtggcacgac atcaacctca acatcctgga attcctcgag agcgttcccg
     5281 acgagcagca gatccgggtc cgcggcgagg acatcatggc cgatccgcac acccggctcg
     5341 ccgaaatcgc gcgctggctc gggtggcgtg acgaccccga ggccatcgag gaaatgatgc
     5401 accccgagcg ctcgccgttt gcctgcatcg ggccttggac cgcgatgttc ggcaacgatt
     5461 cgaattttct ttccggcccg accttcaggc cgcacaagcc gaagatcccg aaggccgatg
     5521 cgccgctgcc gtggcgtacc gacggccagg gtttgtatcc ggaagtcgtg tcattgatgc
     5581 gcgagtttgg ctatgacgac tgacactgaa acagacggtt ccgccgcacc cgaacagaag
     5641 gcgctcccgg cgccgcaatc ttccaagcgc cagcggcttt cgccggtacc ggtgaacgaa
     5701 cgcggtcgcg tcgacccgtc gcagatgaat ccgtaccagg cggcactccg gcagtccctg
     5761 gtgaattcgg tggatgccag gaaggcgcgc cgcttggtgc aacggcagat cggccacatc
     5821 aagtcgagcc gcgacggcgc cgccgccatc aagttcctgt tcgacgaaca cggtcgcgcg
     5881 ccagccaacg cgccgctcga ggacatcgtc gaggagcgca ggcggaccga ataccacttg
     5941 ttgtggctgg aaggtttcgt cgcggaaatg cgcaaccgcc tggtcaagat ccgccgggtc
     6001 gaggatgcgg cgctggaagt ccttgcgcag gcgcacccgg atgaatgaga tccagtccag
     6061 gggggcatcc gggaaccggc atgtgcttgg cagggccgcc ggtcgggtac tggtggccgg
     6121 aatggttgcg gtggcgttgg gcggctgcgt cgtcggcccg gcgtaccacc gcccggacat
     6181 acccttgccg caagcgtggc gcgtgccggc ggggacggcc gacgccgtag tgaacaccgc
     6241 gtggtggaag ggcttccagg acccggacct ggatgcgctg atcaagcagg ccatcgatgc
     6301 caacatggat ctcctgcagg ccacggcgcg cgtcaggcag tacgaggcga aactggacgt
     6361 cgccagtggg gcgcgttatc cggaagcgac ctacgacatc ggcgccgagc gcaggcatta
     6421 cagcaccgcg cacccgaccg ccctcggcat cctcaacgag ccgacccaga acgatttcgg
     6481 cgtcggcatc ggcctcaact gggaactcga catctggggc aagctgaagc gggcggacca
     6541 ggcggcgttg gcagacctgc tctccagcca gtacgcgcgc cacgcggtga tgttgacggt
     6601 cgtgaccaac gtggccacca gttatttcca gctcctggcg ctcgatgatc aactccgcct
     6661 ggcccagcag acgctgagga accgcggcgc cgcgctgaac ctgatcgaga accgttacaa
     6721 agggggttcg tccagcaaga tggcggtgct gcaggcccgc tcggcggtcg acgaggtttg
     6781 ggtcgcgatc cccgacctgg agcggcaaat cgccgtgctc gaggatgcga tgtcgatact
     6841 ggtcgggcgc aatccgggca agatcatccg ccatcgcgac gacgaacccg ccttgccacc
     6901 cgtgcccggc ggagttcctt cggacgtgct gtcgcggcgc cccgatgtgt tggcggccga
     6961 acagcaactc gtcggcgcca acgcgcggat cggcgtcgcc aaggccgctt tgttcccgtc
     7021 gatttcgttg accggcatgt tcggggtggc cagcaatgcg ctcgacaccc ttaccgatgg
     7081 cgatgcgacc gaggcgtcca tcggactcgg attgctgggg cccatctttg ccggcgggcg
     7141 cctgaaggcc ggcatccgcg aagccgaggc ataccagcag gaactgctcg tcaagtatcg
     7201 ccagaccgta ttgaccgcgc tgggcaatgt cgatgacgcg ctcaccttca acgtgaaggc
     7261 gaacgagata gcacggcggg gcgagcaaca ggtcgccacg ttgaacgacc gcctgcacct
     7321 gtccgaagtt cgctacaacg gtggccaggc cgattacctc gaagtcctga cggccgagcg
     7381 cgacctctac aacgcccagg cccagcaggt ggaccgccat cgcgacacct acctggcaat
     7441 ggtttccatc tatggcgcga tgggtggcgg ctggatggtc gaacaggaca agttgcgtgc
     7501 ggcggacaag caggacaccg cctccgccgc gcagtccaag ccgaccacga cgaatcccgg
     7561 cggcaagggg gcgcccgcgg ctgcggcgcc ggccgccgcc atgccggcct ccgcgccacc
     7621 gaccaaagtg aagtcaccgg aatccgcgca atgagcgaaa ccagcaggaa agtcgcactg
     7681 cccgcctacc tcgtgcgccg ctgcgaggcg ttccgcgtgg tcagtggcgc cgagcacagc
     7741 tacgtcgtcc gcgacaaggt gcagaacaag acctgggact tcgatccctg gcagtttttc
     7801 attctggaag tgctgccggg ctgcgaatcc gtggagaagt tgcagacggc attcagggac
     7861 cgcttcaacc gcatcgtcac caatgccgaa ctggaggagt tcttcgcgac ggtcgcggac
     7921 catcggctgt tgaacgaatc ggcgttgcaa catccgctgc tgtcggtgtt cgcgcgccgc
     7981 acctacgacg tcgtggatgg aaagccggtc gccaggcctc acagctcgct gttcgcccac
     8041 aaggactctc cggctgcgcc ttcgatggat tccgaggttg ccgaagcggt ggccgaggct
     8101 caaccggaag aagaggtgct ggccgcgggc gtgcaggacg tgccggggtc ggaccggcgt
     8161 accgcggaca agatgatcga cctgttcgat ccgcgcccga tcctgcggtt cctggcgccg
     8221 gcgctcgcgc cgctgcgcta cgtcggctat gcgtttccgg tgatgttgct ggctgcggtg
     8281 ctggtgttga tccagcactg gcagagtttc gcgaccgatg tcggcggcat ccggatcgcg
     8341 gtgggcctcg gcgggcatct gctgttcgcg ctcctgacgg tgaacctggc ctcggtgatc
     8401 gtgcgcgcct gcatcgcgca caactatgga gcgcaggtcg agagtttcgg aatcatgttc
     8461 ttcgcggggt tcgcgccccg cttcgacgtc ggcatccgcc atctcggcaa gttgagccgc
     8521 gagcagcgca tgtggtacca cggcggcaac ctcttgatac gcggcgtgct gctgtgcgtc
     8581 ggcgtcctcg cctggttctt caccatcgac aagcccggca gtgccgccaa cgacgcgggg
     8641 ctcctgttgg cgctgacgtc gtggacgggc ctggtgatcg aaaccggcaa cccgttcata
     8701 cgcggcagca cctatttcct gcttgccacg tacatggacg agccaaaact gcgcgtcaag
     8761 gcgcgcaggg cgctgatgag caagatccat cgcaaggtct accagcaggc cgacaacaac
     8821 ctcctcgcgg catacggtct cgcgtgcatc gcctacacca cgcttctgat cattttcctg
     8881 acgctcgcgg tgggcaactg gttgtcgaat cgcctcggca tcgggatcgg cgtgtacctg
     8941 gcggcggcgg ccctctgcgg ttacgtggtg tggcgcacct acaagcaact caagaaggtc
     9001 ggcgccatct accagcgttc gcaagagttc gatcgctggc gttcgcgcac gctggtcacc
     9061 gaagacgcgg ccgaaggcga gccggaggtt cccaagaagc attactggcg ctacgtcgcg
     9121 ctgctctgcc tgctgttgct gctgttgatc ccgtaccagt acgacgtcac cggaagcgtg
     9181 gaggtctatc cgtaccgcaa gcagcagatt tccaccgata cgcccgggct gatcgaccag
     9241 gtgaatttcg acggcggtga atcggtcaag aaggggaccg tgatcgcaac gctggcgcac
     9301 gaagactaca actcccagat aaaggtcacg gaagcggaga tcgcggcgca ggcagcggtg
     9361 gtcgccaacc tgaagtcgtt gcccaagccc caggagatcg aactggccca ggaggcattg
     9421 cgcgtggcgc agacccaggc cagcttcagc gtcgccaagg tgccgcggct ccaggctctc
     9481 tacaaccagg gtgcggttcc gctggaggac ctggaaaccg cgcgcaagga agcccacacg
     9541 gacgtgcagc aggtccagga aaaacaggcg gcgctggcgc tcgccaagac cggcccgacc
     9601 gcggaccaga ttgcggcggc cgaggccaag ctggcgagcc tgcaggcgca aaaggccttg
     9661 tacgaagaca agctcgctcg taccacgctg cgcatgccgt tcgacggcaa catcctgacg
     9721 ctgcacctga aagaccgcat caacagctac ctcaaccagg gcgacccgtt cgccgaggtc
     9781 gagtacaccg gcaaggtgac cgcgcagatc gacatcgacg aatccgatct ccagtacgtc
     9841 aaggtcggtt ccacggtgcg gttgcgcccg accgcgtatt tcaaccggga gttcgatggc
     9901 gtcgtcacca agatcgggca gagcgtgacc agcacgccca ccggaaccta cgcggcggtg
     9961 atcgccacct tccagaacga agatggctca ttgaagaccg gcatgaccgg tgaagcgaag
    10021 atcgacggtc cgaccatacc ggtctggcgc gcgttcacgc agtcgatcca gcgcttcgtg
    10081 caagtcgagg tgtggtcctg ggttccctga cgctggcggg ctgtacgtat cgcaatgccg
    10141 accacggtcg agcaaggaag ataccgatga accagtacac gaggcacgat cgtcagtgtg
    10201 atccggtcgt cctgaccgat tatttcgcga ccatgcgcca tttcctcgag acgcagggac
    10261 gcatgatgtc gatgtgggcc gggcatgccg ggcatgccgg gcgagccgcg gagatcggct
    10321 cggctggctt ggccgacgcg cggcgggtgc cggcaaaggt gaatccggtt cgcccaggcc
    10381 ggccggcggc ggtgcgaggg ccggcgatgg caccggccgc ggttcggccg ccgaagcgcg
    10441 ccggaaccag cgtggcaggt gcacgcgccg tgatgcgcgc gcaggccgag ccgattccac
    10501 ccggcgccgc gcgccggctc tcccggggac gcttcgtcct gacccgggat cggttgggcg
    10561 tggccgatcg gctcgcggct gcattgcgcg cgcgcggggc cgaggtggtg gcgatcgaac
    10621 ccggcgtcct ggccaacgag gacgccttgc tgcagcggtg cgaggccctg cgcgcggagg
    10681 cgccgatcgc cggcatcgtg cacctggctg cgctcggcat gccggcgatc aacggcgagg
    10741 agtcgccttc cacgtggcgt cagatgctgc aggaaaacga aaaggcattg ttcctgctgc
    10801 tgcgcgggct gtcgccgaag ctgatcgatg acgcgcatgt cgtggtcgcc agcgacctcg
    10861 gcggactctt cggccgcgaa ggcagcagcg atggcgcgtt gcggctcggc gccggtggca
    10921 tcggaacagt gaaatcgctc cgcgcggagc gcgaatcgct gcgcgtcaag gcgatcgacc
    10981 tggatccgcg gcgggaagcg gctgcgctcg cggccgatct gctcggggaa atcgagctcg
    11041 atggcggtcg ccaggaagtc ggctatccgg atggccaacg cacggtgttc cgcaccaccc
    11101 cggagccggt gccgacggat ccggcgacgg tcgcggcgag ccgcgggctg gtggtgctgg
    11161 ccaccggcgg cgcacgcggg atcaccgcgg aaacgctgcg cgagctcgcg cgtccgggca
    11221 ataccctggt cctggtcggg cgcagcccgc tcgccgggga gcccggcgag ctcgccgcac
    11281 ttgccggaga agcagccttg cgggagcact tcgtcgcaca agtgcgcgcg ggcaaggcca
    11341 agtggaatcc acgcgaaatc ggcaagcgcg tcgccgcggt tctggcacaa cgggagatgc
    11401 gcgccaacct cgacgatctt cgcgaacggg gcgcgagcgt tgagtatgtc gcggccgacg
    11461 tcaccgatga agacagcatc cgcgcactgg tcgccgaagt gacgcagcgg cacggaccca
    11521 tctccggcgt ggtgcatggc gccggtgtca tcgaggacaa gctgctcgcc gagaagtcca
    11581 gcgactcgtg gtcgcgggtg gtggaaacca aggtgatcgg cctgctgctg ttgcagaaat
    11641 acgtcgactc gcgcgcactc aagttcttca cggtattcag ttcggttgcg gggcgctacg
    11701 gcaacagcgg ccagtccgat tacgcctgtg ccaacgaact gatgaaccgc ctgtgcgttg
    11761 cgctgcaggc ccgctggggc gacaacgtcg cggtcagcgc actgtgctgg gggccgtggg
    11821 gcgcgacccg gttcggcgcg ggtatggtga cggccgatac cgaagccaag tttgcgcggc
    11881 gcggcgtcaa gctggtgtcg gccgagctcg gccgcgccct gttccgcgac gagatcaccc
    11941 gcgcgccggg tgcgcccgtc gagatcgttt gcggggaggc gccctgggat cgacgcgaag
    12001 ccgaactcgg cgtgatcggc accggcagcg cggaggcgtc atccgtcgat gccgccaaag
    12061 ccgccgttga taccgccgcc gctgccggca cggaaccgct gctgggcgac ggcgtctggg
    12121 acgcacaacc gaccggtgag cgcagcgttt cggtcgaact cgaccgcgcg cgacacctgt
    12181 acctggacga acacatgctg gatggcaagc aggtgctgcc ggccgccgcg gcgctggaga
    12241 tgatggccga agcgggccgg agcctgtggc cgggctggca ggtggcggaa gtgcgcgagc
    12301 acaagctgat gaaaggcgtg gagatgcaat cgcggccgcg ccggctcgcg gtgcttgtct
    12361 acccgccgac ctacggcagc agcgaaggtt tcgaggtcac ggccgagctc aagggcgagc
    12421 ttgcgcccgg gaagatggtg acccactacc gtgcggtctt gcacctggcg cagagccttc
    12481 ccgacccggt gcccgtcgaa cgcggccgtc acacggaaaa ggccctggcg gtcgccaagg
    12541 cgtatggcga atggctgttc cacgggcccc gcttccaggt gatcgagagc atcgacggac
    12601 tgtcgcgcac cggcgccggt gcgcgggtcc gggtgtcgcg tcccggcgag tggctcgcgg
    12661 gatgcgcggc agaagggccg gcatgggtct tcgaccccgc gttgctggac gcggccgcgc
    12721 agatggcgtg gctgtggtcg cgggcattcc gcgacgaagc cgcgttgccc acccgtttcg
    12781 ggcgggtgac gcgccaccgc gcgagctgcc cggaacgcat gcacatggaa tattcgcgta
    12841 ccgatacgga ggatccaagc ctgatccgcg gcaacgtcac gttcctcgac gagcacgggg
    12901 agccggtgct ggcgatcgag gaactggaca gcatcgccag cgccgcgctg aaccgcttcg
    12961 gtggcaccgc gactgccggg cagaaggtga cgtcatgaac cgcacggttt cccaacgtcc
    13021 cgctccggcc cccatcgcca tcatcggcat ggcctgcatc tttccgcagg cgccggacct
    13081 cgcggcgttc tggaacaaca tcctcgcggg cgtcgatgca attgacgacc cggtgccgga
    13141 gtggggcgct gaacgctacc ttgcggccgg tcgcatccgc accgcgcgcg gggggttcct
    13201 caaggacctc taccgtttcg atccgcgcga attcggcatc atgccgaact cggtcgatgg
    13261 cggcgaatcc gaccagtacc tcgcgttgcg cgtagcccgg gatgcactgg ccgacgcagg
    13321 ctacctgggg gccgacgccg accaccacgc caccggcatc gtgctgggcc acagcgccta
    13381 cctgcatcgc ggccaggtga ccgtgatcca gaacaacatc gtgctggacc agacgatgga
    13441 cttgctgcgc gtcgcgctgc cgcacctggg cgatgccggg ctggacgaga tccgggaaat
    13501 cctccggcag aaattgccgc cgaccacggc cgacaatgcg ccggggctgg tcccgaacat
    13561 gatgacgggc cgcatcgcga accgcttgaa cctgcgcggt ccgaattacg tgctcgacgc
    13621 agcctgcgcg tcctcgctgc tggcggtcgc ggccgccgcg gacgagctgc gcaacggccg
    13681 cagtgacatg atgctggccg gcggtgtcaa cgcgaccttg ccggccgacg tcaccaccag
    13741 cttcacgcag ctcggcgcgc tgagcgcccg cggccgcgtg cggcccttcg aggccggtgg
    13801 cgacggcacg ctgttgggtg aaggcctggg cgtggtcgtg ctgaagaggc tcgacgacgc
    13861 cattgcgcag ggggatcgcg tgtacgcggt attgcgtggc gtcggccgtt ccagcgacgg
    13921 ccgcggcacg ggcctgctgg cgccgagcca ggatggcgaa acgctggcca tgcagcgtgc
    13981 gtatcgacag acgggcatcg acccggcgac gatcgatctc gtcgaggcgc acggaaccgg
    14041 cattccgctc ggcgaccgga ccgagatcgc ttcgctgaaa accgtgttcg gcgaccgggg
    14101 ttcggagttc ggcaccaagg cactcgggtc ggtcaagtcg atgatcagcc actgcatccc
    14161 ggcggccggc attgcgggtc tgatcaaggt gtcgatggcg ctgcaccatc gcgtactgcc
    14221 cccgacccta tgcgacgaag tcaatccgga actgggcatc gagcagacgc cgttctacgt
    14281 caacacgcgg tgcgccccat ggatggcgcg gcacggtgcc ccgcgccgcg ccggggtcaa
    14341 ttcgttcggc ttcggcggca tcaatgcgca cgcgatcctc gaggaggcac cgcccgaagc
    14401 gcgtgccccc ggccgcttca cctcgtggcc cgccgaactt tgcgtgttgt cggcggcgag
    14461 cgccgcggcg ctcgtggaaa agctggagcg gctcgtcgcg gcgctttcgg cgaacccgca
    14521 gtggagtatc gcccaggtgg ccgccgcact cgcgcgcgag gatcgcgccg aggagcaccg
    14581 cgtcgcgatc ctggcgcgcg acaccacgga actgaccacc agcatcgcgc tggcgatcgg
    14641 caaggtcggc gaaggggcgt ccacctggaa ggcgagccgc gcgcgacagg tatacggccg
    14701 gggccacagc gatgccaggc tcggcttcat ctttcccggg gaaggttcgc agtacccggg
    14761 catgttcgcc gacatcgcgt tgttcccgca ggtccagcac tggctggatt tctggcatgg
    14821 aatgcacgac ctgccggcgg ggcagaccca taccgacatc gtgtttccca cctgcgaagt
    14881 ccggcatgaa cacctcaccg ggcgggagca acgcctgcac gacatggacg tcggctccga
    14941 ggcagccttc atcggcggca tggcgatgtt cgacctgctg cgggcgctcg gcgtcgagcc
    15001 ggatgtgatg gtgggacaca gttccggcga atccgcggca ctggcggcat ccggcgcgag
    15061 ccccgcgcac agcgacctcg agctcgtcgc ctgcatcagc aggcagtacg ccgtgtacga
    15121 ggctttgttg aaatcggaca agatcccgac cggcgcgttg cttgcggtgg gcgcattgcc
    15181 gggcgaacag atccaggccg cggtcgattc gcacggcggc aaggtgcagg ttgcgatgga
    15241 caattgcagc aaccagcagg tgctctacgg atcggtcgaa gccatcgggc agatcgagga
    15301 tgagctggtc aggcagggcg gcatctgcat ccggttgcca ttcgatcgcg gctatcacac
    15361 ccccgcgttc gaggacgcga gccgcgcgtt cctcgcgtac tacgaagaca tcaaggttgg
    15421 ccgcccgaag gtaccgttgt attcctgcgc cacggcggct cttttcccga agacccccgc
    15481 cagcgtgcgc aagctcgcag cgcagcagtg gtcgcgcaag gtgcgttttc ccgaaacggt
    15541 ccggaaaatg tacgacgacg gcgtcaggat cttcctggag gtcggtccgt ccggcaacct
    15601 agtttccttc gtcaacgaca tcctggccgg acgcgacttc cttgcactgc cgaccaatgt
    15661 ccgcgggcgc aacggcatcg agcaggtgct gtcggtgctc gcgcggctgt atgcactcgg
    15721 ccgctggaat gcgctggaca gggtgtacgc cggccgcgag atcacgccga tcgagctcgg
    15781 ggatcgcagc cccgcggcgc ggcccccgtt gctcgccaac accatgccga tggtgcggct
    15841 tggaccggcc gactgcgaaa ggatcggcgc tttggccgcc gtggcccgga cgcccggtcc
    15901 gaccgcgagc gcggacggat ccgggctccc cggcgcagag gttggcggag gcgaggacgc
    15961 ggccgccagc acgggacttc ccccgggagg cgagggacac gcgcattcca cccatgcgaa
    16021 cgacagcgcc acgccgctcc tggatgtggt gctggaactc gacgaaaacc acatcgtcgc
    16081 gctctgccgt gtttcgctgg aacacgacca gttcatccgc gaccatgtga tttcgggtcc
    16141 cgtgtcggtg gatcctgcg
//
LOCUS       DASSNH010000298        26968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_197906, whole genome shotgun sequence.
ACCESSION   DASSNH010000298 DASSNH010000000
VERSION     DASSNH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 26968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26968
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197906"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="glnA"
                     /locus_tag="VFK00_11035"
     CDS             <1..793
                     /gene="glnA"
                     /locus_tag="VFK00_11035"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438800.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET7593524.1"
                     /translation="VEVHHHEVGNAGQCEIGVRFNTLLHKADDLQTMKYVIRNVAHAN
                     GKTVTFMAKPVVGDNGSGMHVHQSLSRDGKNLFAGELYGGLSQTALWYIGGIFRHARA
                     INAFANSTTNSYKRLVPGFEAPVMLAYSARNRSASCRIPYVSNPKGRRIEVRFPDPMN
                     SGYLTFTALMMAGLDGILNKIDPGAPMDKDLYDLPPEEERNIPQVCSALDTALEALDR
                     DREFLKAGGVMSDDFIDAYIALKMKEVTAYRASTHPLEYQMYYSI"
     gene            875..1357
                     /locus_tag="VFK00_11040"
     CDS             875..1357
                     /locus_tag="VFK00_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HET7593525.1"
                     /translation="MIRTASIADAPAICTIYNHYVTDTVVTFEEQPVAAHEMRARIDG
                     VLERLPWLVLEHAGGVAGYAYASPWKSRTGYRFAAESSIYVAPAHLGRGFGSALYEHL
                     LGDLRARGIHCVIGGAALPNPASIALHEKFGFTQVAHFRENGCKFGRWIDVAYWQLML
                     "
     gene            complement(1391..2731)
                     /locus_tag="VFK00_11045"
     CDS             complement(1391..2731)
                     /locus_tag="VFK00_11045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7593526.1"
                     /translation="MSNERHGVRDPIRGLVCLLAVSACLLLWHHYGMNRSLRLTAADH
                     YAVWPYDDRDMGGKSVAHVTKTRSFIGLHCKLSAEYAAPYCGFSISLTEGVHGVDLSR
                     FDTIALDIDTPGMDRLPVRVYVRNFDPRYARKGDPASLKVNQMQYVPQREAHPFVLPL
                     KIFQVAQWWIASHNIAPEESMPDLHNITVVEVATGDFTLAGDYTVRVKSISFHGKWIS
                     TLQLLSAILALWVVAALLFVADSTIRSHRAMRAIQQENRELENLNAALELQRQELETA
                     ATHDDLTGVYNRVGLRSSLYRLVLRARDNEGALSIIFLDVDHFKAINDRYGHLAGDEL
                     LAELAAFLKGHTRDGDCLCRWGGEEFVLLCADAQLQVACRLAEKLRLLISDHAWSNGV
                     ALRCSFGVAQMERDEDIGKVFRRADAALYRAKQHGRNRVVCSAGTGPMGQDQAA"
     gene            2976..3635
                     /gene="alkB"
                     /locus_tag="VFK00_11050"
     CDS             2976..3635
                     /gene="alkB"
                     /locus_tag="VFK00_11050"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HET7593527.1"
                     /translation="MTPNTSLALFDMPPAPEREELAAEAWVLRGLAAGRAESLLADIQ
                     RIAAHSPFRHLVTPGGKTMSVAMTNCGAVGWYSDRRGYRYVERDPETGQPWPAMPALF
                     AELARGAAHEAGFSDYAPDVCLVNRYEVGTRLTLHQDRDEHDRHAPIVSVSLGVPAIF
                     LFGGLKRNEPRRRVPLAHGDVVVWGGASRMRYHGVLPVNAGEHPLTGPRRFNLTFRVA
                     R"
     gene            3701..4690
                     /locus_tag="VFK00_11055"
     CDS             3701..4690
                     /locus_tag="VFK00_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7593528.1"
                     /translation="MEQLATGIARLDGGGRMAYVNPAFAEQTGVGLSRLLGQTLATLK
                     PHGERLHERALQARAGSTALALHDIAVCATPGREARLDFTFTASDEAVWVEVHPAAPD
                     MGTSRVSESLRGFAHEIRNPLAAISGAAQLLEQRAGESGQRELALLIHAEAVRLAKLS
                     ERLLGGRNAMAIRAVNVHAMLERAAELLEAESIGVAVVRDYDPSLPAWHGDADRLLQA
                     ILNLVRNAVEANAGRIVLRSRAEAGWRDAHGQHVPALRIEVEDDGDGVPDAIATTLFE
                     PMVSGRPDGTGLGLALAREIAREHGGELALRPRPSGACFVLLLPRHPRMGELE"
     gene            4687..6111
                     /gene="ntrC"
                     /locus_tag="VFK00_11060"
     CDS             4687..6111
                     /gene="ntrC"
                     /locus_tag="VFK00_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HET7593529.1"
                     /translation="MSTIHVVDDDRAVRFVLAMALRDAGHVVKEFADAEAARRAMRQA
                     TPDLLVCDVRLPGTGGLALLREVKAAHPELPVVVMSAFTDVATTAAAYRDGATDYLPK
                     PFDITHAQATVQRALTASARPETATRTGQPRHALLGESAPMREVFRLVGRVASSDLNV
                     LITGETGTGKELVARALHEESARRSKPFVALNTAAIPAELLESELFGHEAGAFTGAQR
                     RAAGRFEQAEGGTLFLDEIGDMPLALQTRLLRVLAGGEFYRVGGRELIRSDVRVLAAT
                     HQDLDARVRRGEFRADLMHRLDVVRIHLPALRERRADIPLLARQFLMAATAELKLPEK
                     QFTPPALQALSRSDFPGNVRQLENLCRRLAVVAPGASIRREDLEDAGSRAGPLPPQSE
                     LPWEAALRAWADAELAAGASDLHVRAREHLDRVLFDAALAAHGGHRQNAAKALGLGRN
                     TLTRKLGSSRGSRDRGPGTRDRRS"
     gene            6230..6697
                     /locus_tag="VFK00_11065"
     CDS             6230..6697
                     /locus_tag="VFK00_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7593530.1"
                     /translation="MPTPLTIRAATTADIPFLVECNAAMALETEHKALVRDTLTRGTR
                     AVFDDPCRGFYLVAERDGEVAGCLLVTYEWSDWRNGDWWWFQSVYVVPAARRSGVFRA
                     LYAEVERRARAAGAVGLRLYVERDNARARQTYLSLGMEEEPYRMLRRGFIEFL"
     gene            6805..8091
                     /locus_tag="VFK00_11070"
     CDS             6805..8091
                     /locus_tag="VFK00_11070"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7593531.1"
                     /translation="MIAQTRRVAVLGGVRIPFCKRDTAYADLGNFGMSVEVLGALVEK
                     FGLHGVELGEVAFGAVMNHPADWNLAREAVQSSGLAMTTPGFTTARACGTSLDNAILV
                     AQKIATGQIEAGIGGGSDTTSQVPLTYSNRLQQRLLDFNRARTFGQKLRAATHDFHFR
                     ELKPQVPGVVEPRTGLSMGEHCEKMAREWKIGRAEQDRWALESHRKLAAAYDAGFFDD
                     LVVPFRGVARDNLLRADSTLEKLAALKPAFDRRSGKGTLTAGNSSALSDGAAAALLAS
                     EDWARERNLPVLAYLTDAEVAGVDYVHGEGLLMAPTIAVPRMLERACLKLQDFDFYEI
                     HEAFAAQVLCQLRAWESADYCKTRLGLDAPLGSIDPAKINVHGSSLATGHPFAATGAR
                     IVATLAKLLAQKGSGRGLISICTAGGMGVVAILERP"
     gene            complement(8247..8819)
                     /locus_tag="VFK00_11075"
     CDS             complement(8247..8819)
                     /locus_tag="VFK00_11075"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET7593532.1"
                     /translation="MPMDINIHLSDDDLQYFIKGMHDVEASVRETPHDEIIAAAQDLL
                     ESTRGASVPPFIAERLGSVESLISMARDVGFGLPEADCQRVLAALAYLADPKDAIPDA
                     VPVLGFLDDAIMIELCRQDLRFEIEAYDDFCAWRADEARARGIDPEKLMAQRADWADA
                     RAAEAITLMHRRRRDSYASGAWRPTLFKVG"
     gene            complement(9013..10845)
                     /locus_tag="VFK00_11080"
     CDS             complement(9013..10845)
                     /locus_tag="VFK00_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET7593533.1"
                     /translation="MNRRFRTRLGVCLCVFTLALAACAPLSPRRIQRADRIVAMSVDH
                     RVDCPRTDHCADPSPFLEQADTAIAASTRGHPRNFVTLLNIGQNALVARLNLIRAAHK
                     SIDIQTYIWVDDDAGLLVLDELVKAARRGVVVRVLADQLGSFNKPELLSQLARASSNL
                     HIELYNPTFDSAHSNFVEYTASALCCFHKFNQRMHNKLFLVDGKVGIAGGRNYEDKYY
                     DWDSQMDFLDRDVLVAGPAGRQMEKSFDIFWHNDRSVPLTHLKDVNAEILKHPFDARP
                     WTEPRFGDPQRVAKVRAQAGDADWLKANLLDHSLEVGLADYFYDLPSKDEAVHGPDSR
                     ELTRDLMAMLSGAQHEIVLQTPYLVLTDRAGKIFKALQKKKPPVDVIVSTNSLASTDA
                     LAVYALSYKHHKKFLVDFGFRIHELMPHPADGPEMLADYNRLSGIGSDVPTHRWGRAP
                     IEAGHAGPRISLHAKSLVVDGAIAMVGSHNFDPRSDNYNTESGVIIDDRAFAQQVRGS
                     IMRDAAPRNAWLVAPRHPIPIIGDVSRAIGDVSRALPIFDIWPWGYATDYQLKPGGTP
                     VPPTDPKFLDNWKPVGDFPEVNLSLTAIITRFITAFGAGMQGIL"
     gene            complement(10868..11446)
                     /locus_tag="VFK00_11085"
     CDS             complement(10868..11446)
                     /locus_tag="VFK00_11085"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7593534.1"
                     /translation="MKAGLALALLLLPAYALATLPPPMRTWPIDPDGSEVQFSVRKLW
                     FAHERGTFPQLHGTLRRIGSGAAEDVLEVDATVVAADLQMDDPEHRVRALGPDFFDAA
                     RFPWIRFESEPFAQDELANGGPVHGLLTLHGVRRPVTLALQASKCPAQPLACTIRVRG
                     TILRANFGMQALRGVLANRVELDLRIMVGAGN"
     gene            complement(11443..11916)
                     /locus_tag="VFK00_11090"
     CDS             complement(11443..11916)
                     /locus_tag="VFK00_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7593535.1"
                     /translation="MRELLLLRHAEAMSAGPDGRDIERPLSLRGERQARAVGEWLADQ
                     PSPDAVLCSAARRAQMTCDELSRFARIPSARLLPDIYRASPGELLSLVEAHAPQARRA
                     MIVGHNPGLEQLLALLTEGRSDAARGLTPATLAWIAFDEDALEPGRGRLHALWTP"
     gene            complement(11929..12957)
                     /locus_tag="VFK00_11095"
     CDS             complement(11929..12957)
                     /locus_tag="VFK00_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593536.1"
                     /translation="MPDASAPEAPASVPATAASSGPASDLPPADQKRVAAIQQYQHDL
                     VTVVALRAEPDYLLGAAILARPFGDSSAGLDFDSLSTRAAAAPGAGAAEHWVRLVACK
                     GEEDCPNRGALAWLEKHAADNAAVWVVAMDAAALKDDPKAEHAALVKAAAAGVYDDYY
                     GKALAGTAMAVAVLPPLPDTMAGAHDGQPDNPEGVRVLVSVNATQASPRPDLGPVVKS
                     CEPDAVGKHKDTRQACLKLAHTLQWGSSPVARAAGLHIQAQLETGADTANQQSTRDLA
                     WQVHQYSALLQRSLTEPTQASRWLATARNGGTELSLILATLRANKVPLNAPSDQAAAP
                     ASGGSADP"
     gene            complement(13505..14356)
                     /gene="speE"
                     /locus_tag="VFK00_11100"
     CDS             complement(13505..14356)
                     /gene="speE"
                     /locus_tag="VFK00_11100"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET7593537.1"
                     /translation="MPPSNWFTEQHEASGSSIGYRTHAKLHEEQTPFQHIEIWSTTDW
                     GNLMVIDGCIMLTSRDNFLYHEMMTHPALFTHAAPRDVVIIGGGDCGTLREVLRHGGV
                     QSAVQVEIDERVTRLAEEYFPELCESNGDPRAQLLFDDGIRYMADCAPGSLDLVIVDS
                     TDPVGPAEGLFNAKFYESCFRALREGGILVQQSESPMVLLDLIRSIHAATRGVGFSDV
                     RTLPFPQPCYPTGWWSCTMARKGGAFDGFRERDAANKSFPTRYYNADIHRAALAQPEF
                     MRQALAG"
     gene            14448..16349
                     /gene="speA"
                     /locus_tag="VFK00_11105"
     CDS             14448..16349
                     /gene="speA"
                     /locus_tag="VFK00_11105"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET7593538.1"
                     /translation="MSQAWTLDDARETYSVAHWSDGYVDIDGDGRTVMRPCGAHGPAI
                     ALEDAVARARGMGLRLPLLLRFPDILADRREKLRAAFAAAMRARSYAGGYTALYPIKV
                     NQQRAVAEVLAHAPASSRSDGTGFGLEAGSKPELLAVLGLAAPGSLVVCNGYKDADYI
                     RLALIGRKLGIDVVIVIEKPSEFEHVVHESRELGVEPKLGVRVRLASIGAGKWQNTGG
                     DKSKFGLTPAQLLALVARLREAGLTHAMRLLHFHMGSQISNLRDIGAGMREAVRHFAE
                     LRAAGLPLDHVDVGGGLGVDYEGTRSRGECSINYDLDQYADAIVAPLAAACADLQLDP
                     PHVLTESGRAMTAHHAVLVVNVSEVERAPQGEVPPAAENEPGVLRSLREALAALGERP
                     VRELWQDAQQYLNEGHLLYSQGQLDLAQRARLDELYHAVALGVRKRLKPEERSHREIA
                     DVLDMKLVDKYFCNFSVFESVPDVWAIDQVFPIMPLARLGERPTRRGALADLTCDSDG
                     RIDVYVESGGLDVSLPLHELREGESYRLGIFLVGAYQETLGDIHNLFGDTDAVNVRID
                     GDGFALDGARRGDSADMLLDYVGYRLDDLRAVYGERIAAAGIEGAAAQRMEAALEAGL
                     TGYTYLRET"
     gene            complement(16360..18534)
                     /locus_tag="VFK00_11110"
     CDS             complement(16360..18534)
                     /locus_tag="VFK00_11110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7593539.1"
                     /translation="MAEWVAFFHELRRRHVWRVAAAYAVAAWLLVQVATQVFPFFDVP
                     NWVVRVVVLLLALGFPVTVGLAWVYELTPDGLRRTEPANPHGVHSTPAARRVKRKLNT
                     ILITLLVIAVAVLGARLLVLQRAPARVAASPPAQSPTGTGTAVQPAQPIPARSVAVLP
                     FQNLSDDKGNAYFAAGMQDLILTKLAEIGDLKVISRASTQQYATRPADLKVVGDQLGV
                     AAILEGSVQRQGNQVLINVQLIDSRTDSHLWAQAYHRTLDDVFGVEGEVADKVAQALK
                     ATLSPDETAKLAVVPTHDRKAYDLYLRAEYQADKGFTNYDTASMKAAIPLYQQAVRQD
                     PEFALAYARLSYVQSQLAWFGRMDTGGQRLIADARANAERALGLAPSLPAAHLALGFS
                     DYWGHGDYGAALKAFAAALALRPNDAATLAAQGFVQRRQGRFDAGIDSLEQAFALDPR
                     NSSLAFETGSTYMMTSRYADAERWLRRALAIDPENRNAQVYLSNSILLSTGDIPAALA
                     AAQGDDPALQLQRAGLLMYQRKYADSLALLDAIPDTPDNFSGSTLGSISPKALQQAEL
                     RRLMGDAAAGPALYAKILPTIREQLAQQQGINQAFTWQTLATAELRLGHGEQALDAAR
                     KAEAIVNQTRDFVYGPGLLQQNAALYADAGRPDLAVPPLAKALASPGVGMFYSPVLLW
                     IDPAWDPIRKDPGFQALLKQYEKYKPGITYELASRTRPASSR"
     gene            complement(18624..19379)
                     /locus_tag="VFK00_11115"
     CDS             complement(18624..19379)
                     /locus_tag="VFK00_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7593540.1"
                     /translation="MQGKTILVTGASAGFGAAIAAKFAENGWRVIACARRVERLAALV
                     ERFGSERVHPCAFDIRDEAALRRALDSLPEAFGDIDVLVNNAGLALGTARAQDCDLAQ
                     WRQMIDTNVTALVTLTRLLLPKLVERRGAIVNISSISGTYQYTGGNVYGATKAFVTQF
                     SLGLRTDLHGTGVRVSNIEPGMAETEFTLVRTGGDQAASDKLYKGAHPITAGDIADAA
                     WWIANLPAHLNVNRLEMMPVSQSAAGLQVARQG"
     gene            complement(19512..20135)
                     /locus_tag="VFK00_11120"
     CDS             complement(19512..20135)
                     /locus_tag="VFK00_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7593541.1"
                     /translation="MANQRNGRQAVRNGRSGIPGWGLFLGGVVVGLVLCAIVVWSGHL
                     PSLRRNDQPQANPNAVAEKGSSPGIAGSSTASGASQFDFYKVLPEKEVVIPSTELSAM
                     AKAEQQKAAAANNASVAAPTSETAEPSAPGGYVLQIGSFPDAGKAEAAKAKLALQGFT
                     AHVQTITLDGQTWNRVQIGPFASATELQRVQKQLADAGIHAIPLKEK"
     gene            complement(20320..22083)
                     /gene="argS"
                     /locus_tag="VFK00_11125"
     CDS             complement(20320..22083)
                     /gene="argS"
                     /locus_tag="VFK00_11125"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7593542.1"
                     /translation="MKDLLSSLLLRALESLHREGAEEAAAPDFVIERTRSREHGDFAC
                     NVALLLARAQGRKPRDVAAEIIGVLPENDVVARVEIGGPGFINFFMSDAAWQEEIRRV
                     FHQGMQYGHSQVGNGRAVGVEFVSANPTGPLHVGHARAAAIGDTLARLHSATGWKVYR
                     EYYYNDAGAQIHNLALSVQARLKGIEPDDPRWPADGYRGDYIRDIARDYASGTAVQSD
                     HPRGDYNREYTRELARDDVAETAPDAGAPRVPAGQDPDDLDAIRDFAVAWLRREQDED
                     LRVFGVEFDTYFLESSLYSEGAVQRVVDRIAQQGHCYEEDGALWLRSTEFGDDKDRVM
                     RKSDGAWTYFVPDVAYHLSKWERGYKRAITELGADHHGSLARVRAGLQALDIGIPRDW
                     PEYVVHQMVTVMKGGEEVKISKRAGSYVTLRDLIDMAGLDATRYFMVARKSDSQLVFD
                     IDLARSQTNDNPVYYIQYAHARVCSVHRQMHERSIVFEERNALKHLGRLESTQARDVM
                     VEISRFPEIVAAAAANFEPHLLAQYLRELAGAFHVWYNAEQFLVEDEGLRNARVALAY
                     AVRQTIANGLAMLGVSAPESM"
     gene            complement(22388..23050)
                     /gene="radC"
                     /locus_tag="VFK00_11130"
     CDS             complement(22388..23050)
                     /gene="radC"
                     /locus_tag="VFK00_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7593543.1"
                     /translation="MTIKDWPEDERPREKLLARGAAALTAAELLAILLGSGIRGASAV
                     DMGRTLLAQAGSLSALLTSDLAAPGLGSAKRARLTAALELARRCLGEELTARPALTSP
                     RDSAAFLKAQLAHKPYEVFACLFLDNRHRVLAFEELFRGTLDGASVHPREVVRASLRH
                     NAAAVILAHNHPSGVAEPSAADRNITHQLRDALQLVGVRVLDHLVIGAGEPTSMAARG
                     LI"
     gene            23180..24474
                     /gene="coaBC"
                     /locus_tag="VFK00_11135"
                     /pseudo
     CDS             23180..24474
                     /gene="coaBC"
                     /locus_tag="VFK00_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964144.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            24471..24950
                     /gene="dut"
                     /locus_tag="VFK00_11140"
     CDS             24471..24950
                     /gene="dut"
                     /locus_tag="VFK00_11140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005420510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7593544.1"
                     /translation="MRAGMPGGEVAVHLKRLDARLGDSIPLPAYATDGSAAMDLRAAP
                     EVDIVLSPGDSALVPTGLAIHIADPGWCALILPRSGLGHKHGLVLGNLTGLIDADYQG
                     PLMISCWNRGKLPFTIRPGDRIAQLMVVPVARVAWRVVDEFAASGRGAGGFGSTGVA"
     gene            24955..>26968
                     /locus_tag="VFK00_11145"
     CDS             24955..>26968
                     /locus_tag="VFK00_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7593545.1"
                     /translation="MADKSAVRKSPNVKAAARAVRARLSLRRGLAEVAADIALALGTL
                     LLLFGLFMGWQTWQITRQASATQALARGKQEAAQKITAIVADKRAEVARALAQPAVVA
                     ELATDAGPESLAAAAKQVQAAIDGAPKVTLYSAQLNEVLHGNLQDLGYARAAQLMSAL
                     SDDELGPAQSPVARILSFAGPIVGPDGNVLAYATVDFRDSLMRIAMSTPRISAGRLEL
                     RQTGKSGDVLLDSVGEHGPSLDDDVGVRVPHSLFVITSAPPDYWLPITRSLIPALALA
                     VVALFLGIALLWWRPRLRVRLGGAASAEEPALADVIAAAPRPPAPTPKPVTEPEQADT
                     AAAAAAVQLDNSLPDRGIFRAYDIRGVVGKTLSTGVARQLGRAIGSAVREKELAEIVV
                     GRDGRLSGPDLSDALIEGLRAAGVDVIDIGAVPTPVSYFAAYQLNTGSCVSVTGSHNP
                     PDYNGFKVVIGGETLAEAAIQDLYQRIVEGRLAEGEGNLRQYDIAPEYVERIASDIQT
                     GRSLKVVVDCGNGIAGNTAPAVVHAIGCEPVPLYCDVDGTFPNHHPDPSEPANLSDLI
                     LTVQKTGADLGLAFDGDGDRLGVVTKDGEIIYPDRVLMLFAQDVLVRNPGATILYDVK
                     CTGHLQPLILQAGGSPLMWKTGHSLIKSKMRETGAQLAGEMSGHFFF"
BASE COUNT         4281 a         8839 c         9380 g         4468 t
ORIGIN      
        1 cgtcgaggtg caccaccacg aagtcggcaa cgccggccag tgcgagatcg gcgtgcgctt
       61 caacacgctg ctgcacaagg ccgacgacct gcagaccatg aagtacgtga tccgcaacgt
      121 cgcccacgcc aacggcaaga cggtcacctt catggcgaag cccgtcgtcg gcgacaacgg
      181 ttcgggcatg cacgtccacc agtcgctgag ccgcgacggc aagaacctgt tcgctggcga
      241 actctacggc gggctgtcgc agacggcatt gtggtacatc ggcggtatct tcaggcacgc
      301 gcgggcgatc aacgcgttcg cgaactccac aaccaattcg tacaagcgcc tggtgccggg
      361 cttcgaggcg ccggtgatgc tggcctactc cgcccgcaac cgctcggcgt cgtgccggat
      421 tccgtacgtc tccaacccga agggccgccg catcgaggtc cgcttccccg atccgatgaa
      481 ctcgggctat ctcaccttca ccgcgctgat gatggccggg ctagacggca tcctcaacaa
      541 gatcgacccc ggggcgccca tggacaagga cctctacgac ctgccgccgg aggaggaacg
      601 caacatcccg caggtgtgtt ccgccctcga cacggcgttg gaggcgctgg accgcgaccg
      661 cgaattcctg aaggcgggcg gggtgatgag cgacgacttc atcgatgcct acatcgcgct
      721 gaagatgaag gaagtcaccg cgtatcgcgc ttcgacgcac ccgctcgaat accagatgta
      781 ctacagcatc tgagatgttt cggccccgct cctgcgggag gcggggccgc aggccgagcg
      841 cgtggcgctt gtggagcggc gagtgggatt ccgcgtgatt cgaaccgcat caatcgcgga
      901 tgcgcccgcg atctgcacga tctacaacca ctacgtcacc gacaccgtcg tgacgttcga
      961 ggagcagccg gttgccgccc acgaaatgcg tgcgcgcatc gatggcgtac tcgagaggct
     1021 gccgtggctg gtgctcgagc acgccggcgg ggtcgccggg tatgcgtacg cttcgccctg
     1081 gaagtcgcgc accggatacc gtttcgctgc ggagagctcg atctatgtcg cgcccgcgca
     1141 tcttggccgc ggattcggct cggcgttgta tgaacacctt ctcggcgacc tccgcgcgcg
     1201 gggcatccac tgcgtgatcg gcggcgcggc gctgccgaat cccgccagca tcgcgctgca
     1261 tgaaaaattc ggcttcacgc aagtcgcgca cttccgggag aatggctgca agttcggccg
     1321 ctggatcgat gtcgcgtact ggcagctcat gctgtaaacg gcgtggtccg gcgttccatc
     1381 tggacgctgc tcaggccgcc tggtcctgtc ccatgggacc ggtacccgcg ctgcacacga
     1441 cacggttcct cccatgctgc ttggcgcggt acagcgccgc atcggcgcgc cggaaaacct
     1501 tgccgatgtc ctcgtcccgc tccatctgcg ccacaccgaa gctgcagcgc agggcgacac
     1561 cgttcgacca ggcatggtcg ctgatcaaga gccggagttt ctcggcgagg cggcaggcca
     1621 cttgcaactg cgcatcggcg cacagcagca cgaactcctc gccaccccag cggcacaggc
     1681 aatcgccgtc gcgcgtatgc cctttcagga atgcagccaa ttcggccagc agttcgtcgc
     1741 cggcaagatg tccgtagcga tcgttgatgg ccttgaaatg atcgacatcc aggaaaatga
     1801 tcgacaacgc gccctcgttg tcgcgcgccc gcagtaccag ccgatacagg ctgctgcgca
     1861 ggccgacgcg gttgtacacg ccggtgaggt cgtcgtgcgt agccgcggtt tccagctcct
     1921 gtcgctgcag ctcgagcgcg gcattgagat tctctagttc ccgattctcc tgctggatgg
     1981 cccgcatcgc cctgtgcgaa cggatggtgc tgtcggccac gaacagcaat gcggcaacga
     2041 cccacagggc caggatcgcc gacagcaact gcagggtcga aatccacttc ccgtggaacg
     2101 agatggactt gacgcggacc gtgtagtctc cggccagcgt gaaatcgccc gtcgccactt
     2161 cgaccacggt aatgttgtgc aggtcgggca tcgattcttc cggggcgatg ttgtggctgg
     2221 cgatccacca ctgcgcgacc tggaaaattt tcagcggcag gacgaaggga tgcgcctccc
     2281 gctgcggaac gtactgcatc tggttgacct tcagtgaggc ggggtcgccc ttgcgggcat
     2341 aacgtggatc gaagttgcgc acgtacaccc gcacgggcag cctgtccatt cccggtgtgt
     2401 cgatgtccag cgcaatcgtg tcgaagcgag agagatcgac gccatgtacg ccttccgtca
     2461 gggaaatact gaaaccgcaa tagggcgcgg cgtattcggc gctcaacttg caatgcagcc
     2521 cgatgaaaga ccgtgtcttc gtgacgtgag cgacgctttt gccgcccatg tcgcgatcgt
     2581 cgtacggcca gaccgcgtag tgatcggcag cggtcaatcg cagcgaccgg ttcatgccat
     2641 agtggtgcca cagcagcagg catgccgaaa ccgccagcag gcacaccaaa ccccggatcg
     2701 ggtcgcggac gccatggcgt tcgttggaca aagcagcctc atagaagcgt tcgcgaactc
     2761 gcctgcgaat aaccggcggt ccgaagggaa tccgtcggca ggcgtgcaaa cgtcacgtgg
     2821 cttatcggcg ctgccgtggt cgtgcttgag ggagttgtca attctttagg aaggtatctt
     2881 ttcgtcaaga tccgggccat ccggtgtgcc cgggcgacgc gccagaggcg cgtccgcctg
     2941 acgcacggga tcgtcgactt ggttatcctt tccgggtgac gcccaacacg tccctcgccc
     3001 tgttcgacat gccacccgcc cccgaacgag aggaactcgc ggccgaagca tgggtgctgc
     3061 gcgggcttgc ggcagggcgt gccgaaagct tgctcgcgga catccagcgc atcgcggcgc
     3121 attcgccgtt ccggcacctg gtgacgcccg gcggcaagac gatgtcggtg gcgatgacca
     3181 attgcggggc ggtgggctgg tacagcgacc ggcgcggcta ccgctatgtc gagcgggatc
     3241 ccgaaaccgg ccaaccctgg ccggcgatgc cggcactgtt cgcggagctc gcgcgcgggg
     3301 cggcgcacga agccggtttc agcgactatg cgccggacgt gtgcctggtc aatcgctacg
     3361 aagtcggaac ccggctgacg ctgcaccagg accgggacga gcacgatcgg cacgcgccga
     3421 tcgtgtcggt atcgctggga gtgccggcca tcttcctgtt cggcggactg aagcggaatg
     3481 aaccccggcg gcgcgtaccc ctggcacatg gcgacgtggt ggtctggggc ggcgcatccc
     3541 gcatgcgcta ccacggcgta ctcccggtca atgcggggga gcacccgctg accggcccgc
     3601 gacggttcaa tctgacgttc cgcgtggctc gatgattgcc ggccgcaccg gattgcacta
     3661 gattggtgca atgccgtgca ccacgtcaga cacaatcctg atggagcaac tcgccacagg
     3721 gatcgcgcgg ctggatggcg gtggccgcat ggcatacgtc aatcccgcat tcgccgaaca
     3781 gacgggggtc ggcctgtcgc ggcttctcgg gcagaccctc gcgacgctca aaccccatgg
     3841 tgagcggctg cacgaacgag cgttgcaggc gcgtgccggg agcacggcgt tggcattgca
     3901 cgacatcgcg gtgtgcgcga cgccggggcg ggaggctcgg ctggatttca ccttcacggc
     3961 gagcgacgag gccgtttggg tcgaggtgca cccggcggcg cccgacatgg gcaccagccg
     4021 ggtctcggaa tccttgcgcg ggttcgcgca tgaaatccgc aatccgctcg ccgcgatttc
     4081 gggtgccgcg cagttgctgg agcaacgcgc gggcgagtcc gggcaacgcg aactcgccct
     4141 gttgatccac gccgaggccg tgaggctggc aaagctgtcg gagcgcctgc tgggcggccg
     4201 caacgccatg gccatccgcg ccgtcaacgt acacgccatg ctggaacgcg ccgccgaatt
     4261 gctggaggca gaatccatcg gcgtggcggt tgtgcgcgac tacgatccga gcctgcccgc
     4321 ctggcacggc gatgccgacc gcctgctgca ggccatcctg aacctggtgc gcaacgcggt
     4381 cgaggccaac gccggccgga tcgtcctgcg cagccgcgcc gaggcgggct ggcgcgacgc
     4441 acatggacag cacgtgccag cgctcaggat cgaggtcgag gacgatggcg acggtgtccc
     4501 ggacgcgatc gcgacgacgt tgttcgagcc gatggtttcg ggccgtcccg acggcactgg
     4561 cctgggcctg gcgcttgcgc gtgagatcgc acgtgagcat ggcggggagc tggccttgcg
     4621 accacgcccg agcggagcct gtttcgtgct gttgttgccg cgccatccgc gcatgggaga
     4681 actcgaatga gcacgatcca cgtcgtggac gacgatcgcg cggtgcgctt cgtcctggcg
     4741 atggcgcttc gcgacgctgg ccacgtggtc aaagaatttg ccgatgccga agccgcacgg
     4801 cgggcgatgc gacaggcaac gccggacctc ctcgtctgcg acgtgcgcct gccgggcacc
     4861 ggcggcctcg cattgctgcg cgaggtgaag gccgcccatc cggagctgcc ggtggtcgtg
     4921 atgagcgcct ttaccgacgt cgcgaccacc gcggcggcct atcgcgatgg cgcgacggac
     4981 tacctgccga agccattcga catcacccat gcacaggcga ccgtgcagcg cgccttgacc
     5041 gcatctgcgc ggccggaaac cgcaacacgg accggccagc cgcggcacgc gctgctcggc
     5101 gaaagcgcgc cgatgcgcga ggtgttccgg ttggtcgggc gcgtcgcgtc cagcgatctc
     5161 aacgtgttga tcaccggcga aaccggcacc ggcaaggaac tggtggcgcg tgccttgcac
     5221 gaggaaagcg cgcgccggtc gaagccgttt gtcgcactca acaccgccgc cattccggcg
     5281 gagttgctgg agtccgaatt gttcggccat gaagccggtg cattcaccgg cgcgcagcgc
     5341 cgcgccgcgg gacgtttcga gcaggccgaa ggcggcacgc tgttcctcga tgaaatcggt
     5401 gacatgccgc tggccttgca gacccgcttg ctgcgggtgc tggcgggcgg cgagttctac
     5461 cgcgtcgggg gccgcgaact gatcaggagc gacgtgcggg tgctggccgc gacgcaccag
     5521 gacctggacg ccagggtccg gcgtggcgag ttccgcgccg acctgatgca ccggctggac
     5581 gtggtgcgca tccacttgcc ggcgctgcgc gagcgacggg cggacatccc gctgctggcg
     5641 aggcaattcc tgatggctgc gacggccgaa ctgaagctgc cggaaaagca gttcacgccc
     5701 cccgcgttgc aggcactgtc caggtcggac ttcccgggca acgtgcggca attggaaaac
     5761 ctgtgccggc gcctggcggt ggtcgcgccg ggcgccagca tccggcgcga agacctcgaa
     5821 gatgccggtt cacgcgccgg cccgctgccg ccgcagtcgg aattgccgtg ggaggcggcc
     5881 ttgcgggcat gggccgatgc cgaactggcc gccggtgcca gcgacctgca cgtgcgtgcc
     5941 cgcgagcatc tcgaccgcgt gttgttcgac gccgcgctgg ccgcccacgg cggccaccgc
     6001 cagaacgccg ccaaggcact gggactgggt cggaacacgt tgacccgcaa gttgggcagc
     6061 tcgcgcggaa gccgggaccg gggaccgggg accagggacc ggaggagctg atccaccacg
     6121 ccaaggatcc gcacacttcg acccgaagcg gcagcactcg cttgcgcagg cgcgccgggt
     6181 tgctacgctt ttgctcgcta ctccctgctc gctactccct gctcgctgca tgcccacgcc
     6241 gctcaccatc cgcgccgcca ccaccgccga cattccattc ctcgtcgagt gcaatgccgc
     6301 gatggcgctc gagaccgagc acaaggcgct cgttcgcgac acgctgacgc gcggcacgcg
     6361 ggcggtgttc gacgatccgt gccgcgggtt ctacctggtc gccgagcgcg acggtgaagt
     6421 ggcgggatgc ctgctcgtca cgtatgaatg gagcgactgg cgcaacggcg actggtggtg
     6481 gttccagagc gtgtacgtgg tgccggccgc gcggcggtcc ggggtgttcc gcgcgcttta
     6541 tgccgaggtg gagcgccgcg cgcgcgcggc cggcgcggtc ggattgcggc tctacgtcga
     6601 gcgcgacaac gcgcgtgctc ggcagaccta tctgtcgctg gggatggaag aagaaccgta
     6661 ccggatgctc cggcgcggat tcatcgaatt cctataggag cggccggcgc gcggcgccgt
     6721 cttttgcttt tgcccgcgat ctggccggcc gcgcgcgtgg cgcgctccta caatcaagcg
     6781 cgtgagccgc gaggagtaca cgtcatgatc gcccaaaccc gccgcgtcgc cgtgctgggc
     6841 ggcgtgcgca ttccgttctg caagcgcgac acggcttacg ccgacctcgg caatttcggg
     6901 atgtcggtgg aagtgctggg ggcgctggtc gagaaattcg ggctgcatgg cgtggaactc
     6961 ggcgaggtcg ctttcggcgc ggtgatgaat catccggccg actggaacct cgcgcgcgag
     7021 gcagtgcaat cgtccggact ggcgatgacc acaccgggat tcacgaccgc gcgtgcctgc
     7081 ggcacctcgc tcgacaacgc gatcctcgtc gcgcagaaaa tcgcgacggg ccagatcgag
     7141 gccggcatcg gcggcggctc cgacaccacc agccaggtgc cgctcaccta ttcgaaccgg
     7201 ctccagcaac gactgctgga cttcaatcgt gcaaggacat tcggacagaa gctgcgcgcc
     7261 gcgacccatg acttccattt ccgcgaactg aaaccgcagg tgccgggggt ggtcgagccg
     7321 cgcacaggcc tttcgatggg cgagcattgc gagaagatgg cgcgcgagtg gaagatcggc
     7381 cgcgcggagc aggaccggtg ggcgctggaa agccaccgca agctcgcggc cgcgtacgac
     7441 gccggcttct tcgatgacct ggtggttccg ttccgcggcg tcgcgcgcga caacctgctg
     7501 cgcgcggact cgaccctgga aaagctcgcc gcgctgaagc ccgccttcga ccggcggtca
     7561 ggcaagggca cgttgaccgc gggcaattcc tcggcgctct ccgatggtgc ggcggcggcg
     7621 cttctcgcca gcgaggactg ggcccgggaa cgcaacctgc cggtgctggc gtacctcacc
     7681 gatgccgaag tcgcgggcgt cgattacgtc cacggcgagg ggctcctgat ggcgccgacc
     7741 atcgcggtgc cgcgcatgct cgaacgcgcc tgcctgaagc tgcaggactt cgacttctac
     7801 gaaatccacg aagccttcgc ggcacaggtg ctgtgccagt tgcgtgcgtg ggagtcggcc
     7861 gattactgca agacccgact ggggctggat gcgccgctgg gatcgatcga tccggccaag
     7921 atcaacgtcc acggatcgag cctcgcgacc ggccatccgt ttgccgccac cggcgcgcgg
     7981 atcgttgcga cgctggccaa gctgctggcg caaaaaggct cgggacgcgg gctgatctcg
     8041 atctgcaccg cgggcgggat gggggtcgtc gcgatcctgg agcgtccatg agttctcggg
     8101 cgctgcaatc cggaacgccg aggcgaagcc gcttttgtga aaagtcaggc catggacggc
     8161 cgttctggtc tgcattggca cagaaacgcc agatgcagca ggagattccg tgacggggcg
     8221 atcatggacg atcgcacaag tacgcctcag ccgaccttga acaaggtcgg tcgccacgcg
     8281 ccgctggcat aggaatcgcg gcggcgccgg tgcatcagcg tgatcgcctc ggcggcgcgc
     8341 gcgtcggccc agtcggcccg ttgcgccatc agcttttccg gatcgatgcc gcgggcgcgt
     8401 gcttcgtcgg cgcgccacgc gcagaagtcg tcgtaggcct cgatttcgaa gcgcaggtcc
     8461 tggcggcaca gctcgatcat gatcgcgtcg tcgaggaagc cgagcaccgg aaccgcgtcg
     8521 ggaattgcgt ccttcggatc ggcaaggtag gcgagcgccg ccagcacccg ttggcaatcg
     8581 gcttcgggca ggccgaagcc cacgtccctg gccatggaga tcagggactc gacgcttccg
     8641 agccgctcgg cgatgaacgg cgggacgctc gcgccgcggg tgctctcgag caggtcctgc
     8701 gcggcggcga tgatctcgtc gtgtggggtt tccctgacgc tcgcctccac gtcgtgcatg
     8761 cccttgatga agtactggag atcgtcatcg ctcaagtgga tgttgatgtc cattggcatg
     8821 gcgggtgtcc tcgatgtcgg gcggctgaat gcgccagcgt agcgacagcc ctctggcccg
     8881 tcaatgatgg gccagaggcg tagcggcttt tgtgcaaagt cgggccacgg atggccgttc
     8941 tggtctccaa agtggctgcg acctgcgcgg atccacggcg cccacgcgag cggcgttccg
     9001 gacgaacgca aactagagta tcccctgcat gccggcgccg aacgcggtga tgaagcgcgt
     9061 gatgatcgcg gtcagcgaaa ggttgacctc tggaaaatcg ccgaccggct tccagttgtc
     9121 gaggaacttg ggatcggtcg gcggtaccgg cgtgccgccc ggcttcaact ggtaatcggt
     9181 ggcgtatccc catggccaga tgtcgaagat cggcagggcg cgcgaaacat caccgatcgc
     9241 acggctgacg tcgccgatga tcgggatcgg gtggcggggg gcgaccagcc acgcgttgcg
     9301 cggcgcggca tcgcgcatga tcgacccgcg cacctgctgc gcgaatgccc ggtcgtcgat
     9361 gatcacgccc gactcggtgt tgtagttgtc cgagcgcggg tcgaagttgt gcgagccgac
     9421 catcgcgatc gcgccatcca cgaccagcga cttggcgtgc aggctgatgc gcgggccggc
     9481 gtggccggcc tcgatcggcg cgcggcccca gcgatgcgtg ggcacgtccg agccgatgcc
     9541 cgacaagcgg ttgtagtcgg cgagcatctc cggtccgtcg gccgggtgcg gcatcagttc
     9601 gtggatgcgg aagccgaagt ccacgaggaa tttcttgtgg tgcttgtacg aaagcgcgta
     9661 gaccgccagc gcgtcggtcg aggccagcga attggtcgac acgatcacgt ccaccggggg
     9721 ctttttcttc tgcagcgcct tgaagatctt gcccgcgcgg tcggtcagga ccagatacgg
     9781 ggtttgcagc acgatttcgt gctgggcccc cgagagcatc gccatcaggt cccgggtcag
     9841 ttcgcgcgaa tccgggccgt gcaccgcctc gtccttggac ggcaggtcgt agaagtaatc
     9901 ggccagaccc acttcgaggc tgtggtcgag gaggttggcc ttcagccagt cggcgtcccc
     9961 tgcctgtgcg cgcaccttcg cgacgcgctg cgggtcgccg aagcgcggct cggtccaggg
    10021 cctggcatcg aaggggtgct tcaggatctc ggcattgacg tccttcaggt gcgtcagggg
    10081 aacggaacgg tcgttgtgcc agaagatatc gaagctcttt tccatttgcc gcccggccgg
    10141 gcccgcgacg agcacgtcgc ggtcgagaaa atccatttgc gaatcccagt cgtaatactt
    10201 gtcctcgtaa ttgcggccgc ccgcgatgcc caccttgccg tcgaccagga acagcttgtt
    10261 gtgcatcctc tggttgaact tgtggaagca gcacagggcg ctcgcggtgt attcgacgaa
    10321 attgctgtgg gcgctgtcga aggtcgggtt gtagagctcg atgtgcaggt tgctgctggc
    10381 gcgcgcgagc tgcgacagca gttcgggctt gttgaaagat ccgagctggt cggcgaggac
    10441 gcggaccacc acgccccggc gcgcggcctt gaccagttcg tccagcacca gcaggccggc
    10501 gtcgtcgtcc acccagatgt aggtctggat gtcgatcgac ttgtgggccg cgcggatcag
    10561 gttgaggcgg gccaccaacg cgttctggcc gatgttcagc agcgtcacga agttgcgggg
    10621 gtgcccgcgg gtcgaggccg cgatcgcggt gtcggcctgt tcgaggaacg gcgaggggtc
    10681 ggcgcagtgg tcggtccgtg ggcagtcgac gcggtgatcg accgacatcg ccacgatccg
    10741 gtcggcgcgc tggatccggc gcggcgacaa cggcgcgcac gcggcaagcg ccagcgtgaa
    10801 cacgcacaag cagactccga gcctggttcg gaaacgtcga ttcatcgcgg gaatagtacg
    10861 tggtgcgtca gtttcccgcg cccaccatga tcctcaaatc cagttcgacc cggttcgcga
    10921 gcacgccgcg caaggcctgc atcccgaagt tcgcgcgcag aatcgttccc cgcacgcgga
    10981 tcgtgcaggc gagcggttgg gcgggacact tggaggcttg cagggccagc gtcaccgggc
    11041 ggcgcacgcc gtgcagggtc agcaacccgt ggaccggtcc gccgttcgcg agttcgtcct
    11101 gcgcaaacgg ttcggattcg aagcgaatcc acgggaaacg cgcagcgtcg aagaaatcgg
    11161 ggccgagtgc gcgcacgcga tgctcggggt catccatttg caaatcggcc gcgaccacgg
    11221 tcgcatcgac ttccagcacg tcttccgcgg cgccggaacc gatgcggcgc agcgtgccgt
    11281 ggagttgcgg gaacgtgccg cgttcgtgcg cgaaccagag cttgcgcacg ctgaactgca
    11341 cttccgatcc atcggggtcg atcggccagg tccgcatcgg cggcgggagc gtggccagcg
    11401 cgtacgccgg gagcaacagc agcgccagcg cgagcccggc tttcacggcg tccagagcgc
    11461 gtgcaaccgc ccccgcccag gttcgagcgc gtcttcgtcg aacgcgatcc acgccagtgt
    11521 cgcgggcgtc aatccgcggg cggcatcgga acggccctcc gtcagcagcg ccagcaactg
    11581 ctccaggccg ggattgtggc cgacgatcat ggcgcggcgt gcctgtggtg cgtgcgcttc
    11641 gaccagcgac aacaactcgc cggggctggc ccggtagatg tccggcagca gccgtgcaga
    11701 cggtatccgc gcgaatcgcg acagctcgtc gcaggtcatc tgcgcgcgcc gcgccgccga
    11761 acacagcacc gcatccggcg aaggctggtc cgcgagccac tcgcccaccg cccgcgcctg
    11821 ccgttcaccg cgcaggctca aggggcgctc gatgtcccgg ccatcaggac cggccgacat
    11881 cgcctcggcg tgacgcagca gcaacagttc acgcatggca ggctccgctc acgggtccgc
    11941 gctgccgccg gacgccggcg cggccgcctg gtccgaggga gcgttgagcg gaaccttgtt
    12001 ggcccgcagg gtcgccagga tcaggctgag ctcggtccca ccgttgcgcg ccgtggcgag
    12061 ccaccgcgat gcctgcgtcg gctcggtgag cgagcgttgc agcagcgcgg aatactggtg
    12121 gacttgccag gcgagatcgc gggtcgactg ttggtttgcg gtgtcggcgc ccgtttcgag
    12181 ttgcgcctgg atgtgcaggc ccgctgcacg cgcgaccggg ctcgaacccc attgcagggt
    12241 gtgcgcgagc ttgaggcacg cttgcctggt gtccttgtgc ttgccgacgg catccggttc
    12301 gcacgacttg accactggcc cgaggtcggg ccgcggcgat gcctgcgtcg cgttgacgct
    12361 gaccagtacc cgcacgccct ccggattgtc cggttggcca tcgtgcgcgc cggccatcgt
    12421 gtccgggagc ggcggcaaca cggcaacggc catcgcggtg ccggcgagcg ccttgccgta
    12481 gtagtcgtcg tagaccccgg cggcggcggc tttcacaagc gccgcgtgtt ccgccttggg
    12541 atcgtccttc agggcggcgg cgtccatggc cacgacccat accgcggcgt tgtcggccgc
    12601 atgcttttcg agccaggcga gtgcgccgcg gttcgggcag tcctcttcgc ccttgcacgc
    12661 cacgaggcgt acccaatgct cggccgcccc agcgccgggt gcggcggcgg cgcgcgtgct
    12721 caagctgtcg aagtcgaggc ccgccgacga gtcgccgaat ggacgtgcga ggatcgcggc
    12781 gcccagcagg taatccggtt ccgcccgcaa cgccaccacg gtcaccaggt cgtgctggta
    12841 ttgctggatt gccgcgaccc gcttctggtc cgcgggtgga aggtcggatg cgggtccgga
    12901 cgaggctgcg gtggccggca cgctcgccgg cgcttccggc gctgaggcgt cgggcatggc
    12961 cgtgctcgtg gcagcttgca ccgcagtctg cggcgatgcc gcgaaagcgg cgctcgcgat
    13021 ggatgccaaa gcgagtgaga ggacgacgat ggcggcttgg cgcattgggt cgatccggta
    13081 tcggcaacgg tcgaaggtaa ctactgacgg atgaatgcct acttgccgga gggggccgcg
    13141 ccgggaggct cccggctggg gggtggctcg agtggaacgc cgcgtgcctt caacacggtt
    13201 gcgatcatgg ctatctcgtt gggctgcacg cgggtggcat tctgcaccgt gttcgcaagc
    13261 gcgggatcgc catcgaactc gagtttggag tttctttacc catttcagcg taccccggac
    13321 gaagtgtccg cgccggccgc atgcatgtcg gtggtgctgg tcgtagccgg cgtcgcgccg
    13381 gcgtgcggtg ctgtcgagat cccggatgca gctgcacggc cgatgcggcc gccggcagcg
    13441 gcaaccaact cgatacgtcg aaccggatac cgtgcatccc gctcccctga caacgaaagg
    13501 acaattaccc cgcaagcgct tggcgcatga actccggttg ggccagcgcc gcgcgatgga
    13561 tgtcggcgtt gtagtaacgg gtggggaaag acttgttggc ggcgtcacgt tcgcggaacc
    13621 cgtcgaacgc gccgcccttg cgcgccatcg tgcaactcca ccagccggtc gggtagcacg
    13681 gttgcggaaa cggcagcgtg cgcacgtcgg aaaaaccgac tccgcgcgtc gccgcgtgga
    13741 tcgaccggat caaatcgagc agcaccatcg gcgactccga ctgctgcacc aggatgccgc
    13801 cttcgcgcaa tgcccggaag cagctctcgt agaacttcgc gttgaacagc ccctcggccg
    13861 ggccgaccgg atcggtcgag tccacgatca cgagatccag cgaaccgggc gcgcaatcgg
    13921 ccatgtaccg gatgccgtcg tcgaacagca actgcgcgcg cgggtcgccg ttcgattcgc
    13981 acaattcggg gaaatattcc tcggcgaggc gggtgacgcg ctcgtcgatt tcgacttgca
    14041 ccgcgctttg cacgccgccg tggcgcagaa cttcgcgcag cgtgccgcaa tcgccgccgc
    14101 cgatgatcac cacgtcgcgc ggcgcggcgt gggtgaacaa cgcggggtgg gtcatcattt
    14161 cgtggtacag gaaattgtct cgcgaggtga gcatgatgca gccgtcgatc accatcaggt
    14221 tgccccagtc cgtggtgctc cagatttcga tgtgctggaa cggcgtctgc tcctcgtgga
    14281 gcttggcgtg ggtgcggtag ccgatcgacg agccggaagc ctcgtgctgc tcggtgaacc
    14341 agttggatgg cggcatgcgg cgaaccccgg tggcgatgcc ccgattgtaa cgtccgcccc
    14401 gccacgcgac gttacaattc gcgtttcgcc tggcttccgg aacgccgatg tcgcaagcat
    14461 ggaccctcga cgacgcccgc gagacgtatt ccgtcgcgca ctggagcgat ggttacgtgg
    14521 acatcgatgg cgacggtcgc accgtgatgc gcccgtgcgg cgcgcacggc cccgcgatcg
    14581 cgctggagga cgccgtcgcg cgtgcgcgcg ggatgggcct gcggctgccg ttgctgctgc
    14641 gctttccgga catcctcgcc gaccgtcgcg agaagctgcg cgcggcattc gccgccgcga
    14701 tgcgcgcgcg cagctacgcg gggggctata ccgcgctgta cccgatcaag gtcaaccagc
    14761 agcgcgcggt ggctgaggtc ctggcgcatg cgccggcgtc gtcgcgctcg gacggcaccg
    14821 gcttcgggct ggaggccggt tccaagcccg agctgctggc ggtgctgggg ctcgccgcgc
    14881 ccggttcgct ggtggtttgc aacgggtaca aggatgccga ctacatccgc ctcgcgttga
    14941 tcggcaggaa gctcggcatc gacgtggtga tcgtgatcga gaagccgtcc gagttcgagc
    15001 acgtggtgca cgagtcgcgc gagctcggcg tcgagccgaa gctcggcgtg cgggtgcggc
    15061 tggcttcgat cggcgccggc aaatggcaga acaccggcgg cgacaagagc aagttcgggc
    15121 tcacccccgc gcaactgctc gcgctggtgg cgcggctgcg ggaggccggg ctgacgcacg
    15181 cgatgcgcct gctgcacttc cacatgggct cgcagatttc gaacctgcgc gacatcggcg
    15241 ccggcatgcg cgaggcggtg cggcatttcg ccgagctgcg cgccgcgggg ctgccgctgg
    15301 accacgtcga cgtgggcggc ggactcggcg tcgactatga aggcacgcga tctcggggcg
    15361 aatgctcgat caactacgac ctcgaccagt acgcggatgc gatcgtggcg ccgctcgccg
    15421 cggcctgcgc ggacctgcaa ctcgatccgc cccacgtgct gaccgaatcc ggtcgcgcga
    15481 tgaccgcaca ccacgcggtg ctggtggtca acgtcagcga ggtcgaacgt gcgccacagg
    15541 gcgaggtgcc gcccgcggcc gaaaacgagc caggggtgct gcgcagcctg cgcgaggccc
    15601 tggccgcgct cggcgagcgt cccgtgcgcg agctgtggca ggacgcgcag cagtacttga
    15661 atgagggaca cctgctgtat tcacagggcc agctcgacct cgcgcagcgc gcgagactcg
    15721 atgagctgta ccacgcggtg gcgttggggg tgcgcaagcg cctgaagccc gaggaacgct
    15781 cccaccggga aatcgcggac gtcctcgaca tgaagctcgt ggacaagtat ttctgcaatt
    15841 tctcggtgtt cgaatcggtc cccgacgtgt gggcgatcga ccaggttttc ccgatcatgc
    15901 cgctggcgcg gctgggcgaa cggccgaccc ggcgcggcgc gctggcggac ctcacctgcg
    15961 attccgacgg gcgcatcgac gtgtacgtgg aatcgggcgg cctcgacgtc agcctgccgt
    16021 tgcacgaact gcgcgaaggc gagtcctacc ggctcgggat cttcctggtc ggcgcatacc
    16081 aggaaacgct gggtgacatc cacaacctgt tcggcgacac cgacgccgtg aacgtccgca
    16141 tcgatggcga tggcttcgcc ctcgatggtg cgcgccgggg cgattccgcc gacatgctgc
    16201 tcgactacgt ggggtacagg ctcgacgatc tgcgcgccgt ctacggcgaa aggatcgccg
    16261 ccgcgggcat cgagggtgcg gcggcgcagc gcatggaggc cgcgctggag gccggactca
    16321 ccggatacac ctatctgcgg gaaacctgag gccgcgcggc tagcggctcg atgcaggccg
    16381 ggttcgcgac gcgagttcgt aagtgatgcc gggcttgtat ttctcgtact gcttcaacaa
    16441 cgcctggaat cccgggtcct tgcggatggg atcccacgcc ggatcgatcc agagcagcac
    16501 gggcgagtag aacattccga cgcccggcga agcgagcgcc ttggcaagcg gcggcacggc
    16561 gaggtcgggg cgaccggcgt ccgcgtacag cgccgcgttc tgctgcagca agccggggcc
    16621 gtatacgaag tcgcgggtct ggttcacgat cgcctcggcc ttgcgtgccg catccagcgc
    16681 ctgctcgcca tgtcccagcc tgagttccgc ggtggccagg gtctgccacg tgaacgcctg
    16741 gttgataccc tgttgttgcg cgagttgctc ccgtatcgtc ggaaggatct tggcgtacag
    16801 cgcgggtccc gcggccgcat cgcccatcag gcggcgcagc tccgcctgct gcagcgcctt
    16861 gggcgagatg gagccgagcg tcgatcccga aaaattgtcg ggcgtgtccg gaatggcatc
    16921 cagcagcgcc agcgaatccg cgtatttgcg ctggtacatc agcagccccg cgcgctgcag
    16981 ttgcaaggcc gggtcatcgc cctgcgccgc cgccaacgcg gcgggaatgt ctccggtcga
    17041 cagcaggatg ctgttggaca gatacacctg cgcgttgcgg ttctcggggt cgatggccaa
    17101 cgcgcgccgc aaccagcgtt cggcatccgc gtagcggctg gtcatcatgt aggtcgagcc
    17161 ggtttcgaac gccagcgacg aattgcgcgg gtccagtgcg aacgcctgct cgagcgaatc
    17221 gatcccggcg tcgaaccggc cttgccggcg ctgcacgaag ccctgcgccg ccagcgtggc
    17281 cgcgtcgttc gggcgcagtg cgagcgccgc ggcgaatgcc ttcagcgccg cgccgtagtc
    17341 accatggccc cagtagtcgc tgaaaccgag ggcgaggtgc gcggccggca acgatggcgc
    17401 gaggcccagc gcccgctcgg cgttggcgcg cgcatccgcg atcaagcgct gcccgccggt
    17461 gtccatccgt ccgaaccagg ccagttggct ctgcacgtag gaaagccgcg cgtaggccag
    17521 cgcaaattcg ggatcctgcc ggaccgcctg ctggtagagc gggatggctg ccttcatgct
    17581 ggcggtgtcg taattggtga agcccttgtc cgcctggtac tcggcgcgga ggtacaggtc
    17641 gtaggccttg cggtcgtggg tggggacgac cgccagcttg gccgtttcgt ccggcgacag
    17701 cgtggccttc aacgcctgcg cgaccttgtc cgccacttcg ccctcgacgc cgaagacgtc
    17761 gtcgagggtg cggtggtagg cctgcgccca gaggtgcgaa tcggtcctcg aatcgatcaa
    17821 ctgcacgttg atcaggacct ggttgccctg tcgctgcacg cttccctcga ggatcgcggc
    17881 gacgcccaat tgatccccga ccaccttcag gtcggccggg cgggtcgcgt actgctgggt
    17941 cgatgcgcgc gagatgacct tgaggtcgcc gatttcggcc agcttggtca ggatcaggtc
    18001 ctgcatgcca gccgcgaaat atgcgttccc cttgtcgtcg ctgaggttct ggaacggcag
    18061 caccgcgacc gagcgggcgg gaatcggttg cgcgggctgt acggcagtgc cggtgcccgt
    18121 tggcgattgg gccggcggcg acgcggcaac ccttgccggg gcacgttgca acacgagcag
    18181 acgcgcgccg agcaccgcga ccgcgatcac cagcagcgtg atgaggatcg tgttgagctt
    18241 cctcttgacc cgccgggccg cgggggtgga atggaccccg tgggggttgg cgggttctgt
    18301 gcggcgaagc ccgtccggcg tgagctcgta cacccaggcg agtcccaccg tcaccgggaa
    18361 acccagcgcc agcaacaaca ccacgacgcg aaccacccag ttcgggacgt cgaagaacgg
    18421 gaatacctgc gtcgcgacct ggaccaacag ccacgccgcg acggcgtacg cggccgcgac
    18481 ccgccatacg tgccggcgcc gcagttcgtg gaaaaaggcg acccattccg ccacgttcgt
    18541 gccccgccgg tcccgccggc acatggacag cctgacgagc cgagtgtata gctttttgga
    18601 acgcgatcgg cgcggctggc gggtcagccc tggcgcgcga cctgcagccc tgcagccgac
    18661 tggctgaccg gcatcatttc gaggcgattg acgttgaggt gcgccggcag gttggcgatc
    18721 caccacgcgg cgtcggcgat gtctcccgcg gtgatcggat gcgcgccctt gtacagcttg
    18781 tcggaggccg cctggtcgcc gccggtgcgc accagggtga actcggtttc ggccatgccg
    18841 ggttcgatgt tgctcacccg cacgccggtg ccgtgcaggt cggtgcgcag gccgagcgag
    18901 aactgcgtga cgaacgcctt ggtggccccg tacacgttgc cgccggtgta ctgataggtg
    18961 ccggagatgg agctgatgtt gacgatcgcg ccgcgacgct cgaccagctt cggcagcagc
    19021 aggcgcgtca gcgtgaccag cgccgtgacg ttggtgtcga tcatctgccg ccattgcgcg
    19081 agatcgcagt cctgcgccct ggcggtgccc agcgcgaggc cggcattgtt cacgagcacg
    19141 tcgatgtcgc caaaggcttc cggcagcgag tccagcgccc gacgcagcgc ggcttcatcg
    19201 cggatatcga acgcgcacgg gtggacgcgt tcggaaccga aacgctcgac cagcgccgcg
    19261 agccgttcca cgcgccgtgc acaggcgatg acgcgccagc cgttctccgc gaatttcgcc
    19321 gcgatcgcgg caccgaaacc ggccgaggcg ccggtgacga ggatggtttt tccttgcatg
    19381 tgcgtctctt ccatcgcttg gagcaatccg gcgaccgacc cggtaccggg gatgggtggt
    19441 gtgggcgtat cggagcgggt ttcggcaccg cgatacgcgc tcggatcccc gcccccgcta
    19501 ccgactccgt cctacttttc cttcaacggg atcgcatgga tcccggcgtc ggccaactgc
    19561 ttctgaacgc gctgcagctc ggtggcggag gcgaagggac cgatctgcac gcggttccag
    19621 gtctgtccgt cgagggtgat ggtctgcacg tgcgcggtga aaccctgcag cgccagcttg
    19681 gccttggcgg cctcggcctt gcccgcgtcg gggaacgatc cgatctgcag cacgtaaccc
    19741 cccggcgccg atggctctgc ggtttcggag gtgggcgcgg cgacactcgc gttgttggcg
    19801 gccgcggcct tctgctgttc ggccttggcc attgccgaga gttcggtgct cgggatcacg
    19861 acttcctttt cgggcagcac cttgtagaag tcgaactgcg acgcgccgga agccgtgctg
    19921 ctgccggcga tgccgggcga gctgcccttc tccgcgacgg cgttcggatt cgcctggggc
    19981 tggtcgttgc gccgcaggct gggaaggtgt ccgctccata ccacgatcgc gcacagcacg
    20041 aggccgacga ctaccccgcc caggaacaag ccccatccgg gaatgcccga gcgcccattg
    20101 cgtacggcct gcctgccgtt gcgttgattt gccactgtca tcctcgtgcg gcccgcgggg
    20161 ggtcgcgtca aatgtcgaaa aggaaccgcc gcgctgcatc atcctgcggg acgctgcttc
    20221 gcgccagccc ggcggctgga aaaagccgtt tgcagttgct ccgcggaagt ttcgggagcg
    20281 aagcctcggc gcaaaacagg cggcacccgg ggaagcaggt cacatcgatt cgggcgccga
    20341 gacgcccagc atcgcgagtc cgttcgcgat ggtctgccga accgcgtacg ccaacgccac
    20401 ccgcgcgttg cgcaagccct catcctcgac caggaactgt tcggcgttgt accagacgtg
    20461 gaacgcgccg gccagctcgc gcaggtattg cgccagcagg tgcggctcga agttcgcggc
    20521 cgcggcggcg acgatctccg gaaagcgcga gatctcgacc atcacgtcgc gtgcctgcgt
    20581 gctttccagg cggccgaggt gcttcaacgc gttgcgttcc tcgaacacga tcgagcgctc
    20641 gtgcatctgg cggtgcaccg agcacacccg cgcgtgggcg tactggatgt agtagaccgg
    20701 gttgtcgttg gtctgcgagc gcgcgaggtc gatgtcgaag accagttgcg agtcgctctt
    20761 gcgcgcgacc atgaagtaac gcgtggcgtc gaggccggcc atgtcgatga ggtcgcgcag
    20821 cgtcacgtag ctgccggcgc gcttggagat cttcacctcc tcgccgccct tcatcaccgt
    20881 caccatctgg tggacgacgt attccggcca atcccgcggg atgccgatgt cgagcgcctg
    20941 caggcccgcg cggacccgcg ccagcgagcc gtggtggtcg gcgccaagct cggtgatcgc
    21001 acgcttgtag ccgcgctccc acttggaaag gtggtaggcg acatccggca cgaaatacgt
    21061 ccaggcgccg tcggacttgc gcatcacgcg gtccttgtcg tcgccgaatt cggtgctgcg
    21121 caaccacagc gcgccatctt cctcgtaaca atgtccctgc tgcgcgatgc ggtccacgac
    21181 ccgttgcacc gcgccttcgg agtacagcga cgactcgagg aagtaggtgt cgaactcgac
    21241 cccgaacacg cgcaggtctt cgtcctgctc gcggcgcaac cacgccaccg cgaaatcgcg
    21301 gatcgcatcg aggtcgtccg ggtcctgccc ggcgggcacg cggggggcgc cggcgtcggg
    21361 cgcagtttcg gcgacgtcgt cccgggccag ttcgcgggtg tattcgcggt tgtagtcgcc
    21421 gcgcgggtgg tcggactgca cggccgtccc gctcgcgtag tcccgggcga tgtcccggat
    21481 gtagtcgcca cggtaaccgt cggcgggcca gcgcgggtcg tcgggttcga tgcccttgag
    21541 gcgcgcctgc accgacagcg cgaggttgtg gatctgcgcc ccggcgtcgt tgtagtaata
    21601 ctcgcgatag accttccagc cggtcgccga gtgcagcctc gccagggtgt cgccgatcgc
    21661 ggccgcacgg gcgtggccga cgtggagggg tccggtcggg ttggccgaca cgaattcgac
    21721 ccctaccgcc cgcccgttgc cgacctggct gtggccgtac tgcatcccct ggtggaagac
    21781 acggcggatt tcctcctgcc aggcggcgtc gctcatgaaa aagttgatga agcccgggcc
    21841 gccgatttcg acccgcgcga cgacgtcgtt ttcgggcagg acgccgatga tctcagcggc
    21901 gacgtcgcgg ggcttgcggc cctgcgcccg ggccagcagc agcgcgacgt tgcaggcgaa
    21961 gtcgccgtgc tcgcgcgaac gcgtgcgctc gatcacgaaa tcgggggccg cggcttcctc
    22021 cgccccttcc cggtgcaggc tttcgagcgc acgcagcagc agcgaactca ggaggtcttt
    22081 cacgaggggg cgggagtcgg gacagcgaat cgccatcgta gcgcagccgt tcacgtcgac
    22141 caaatgcagg cctgtggata acttggtgga catccgtttt gcggatcagg gtgcaacggc
    22201 ggcggatgcg gcgttgggtt gccagtcagg tagggatatc caagcgtatc aacaatgtac
    22261 gtgtcgttta acgaaaggac cataaaagca gcggccaagc gcttgtggca ccgtgttgtg
    22321 aataactgac ggcgaatggg cgaaggatgt caaagggctc gcgagatcgg cgtccccgcg
    22381 gcgctgctca gatcagtccg cgggctgcca tcgaggtcgg ttcgccggcg ccgatcacca
    22441 gatgatcgag gacccgcacg ccgaccagtt gcagggcgtc gcggagctgg tgggtaatgt
    22501 tgcggtccgc cgccgagggt tcggccacgc cgctcggatg gttgtgcgcc aggatcacgg
    22561 ccgccgcatt gtgcctgagc gacgcgcgca cgacctcccg cgggtgcacg ctcgcgccat
    22621 ccagcgtgcc gcggaacagt tcctcgaagg ccagcacccg atggcggttg tcgaggaaca
    22681 ggcaggcgaa gacttcgtac ggtttgtggg cgagctgcgc cttcaggaac gcggcgctgt
    22741 cgcgggggct ggtcagcgcc ggacgcgcgg tgagttcttc accgaggcag cgccgcgcga
    22801 gttccagcgc ggccgtgagg cgcgcgcgtt tggcggaacc gagtcccggt gcggccaggt
    22861 cgctcgtgag cagcgcggac aagcttcccg cctgcgccag cagcgtgcgt cccatgtcca
    22921 cggcgctggc gccgcggatg ccgctgccca gcaggatggc cagcagctcg gccgcggtga
    22981 gcgcggcggc gccgcgcgcc aggagctttt cgcgcggacg ttcgtcttcg ggccaatcct
    23041 tgatggtcat gttgcctgcc ggtggcgctt gcatacaagc ggcccgatga tggtcgacgg
    23101 catgcaggcg cgggcacagc ggtcggaatg ccggaatccg gtaagctagc cgcctgccgt
    23161 cctgtcagcg gattccgacg tgaacgcaag cgatcatcgc cccagcgaaa atcggcacgt
    23221 tctgctcggg gtctccggcg gcatcgccgc gtacaagtct tgcgaagtgg tgcgacgcct
    23281 gcgcgatgcc ggtgcggaag tgcgggtggt gatgacggcc ggcgccgaac gcttcgtgac
    23341 cgcgctgacc ttccaggcgc tgtcggggca ggcggtgcgc agcgggctgt gggacgaagc
    23401 cgcggagatg ggcatggggc acctggaact cgcgcactgg gccgatgcca tcctggtcgc
    23461 accggcgtcg gcggacacgc tggccaagct cgcacatggt ttcgccgacg acctgctctc
    23521 cacgttgtgc ctcgccgcga ccgcgccact ggcggtggca ccggcgatga accatcggat
    23581 gtggctgcac cccgcgaccc aggccaacgt cgccaccttg cgcaaacgcg gcgtcgcggt
    23641 gctcgggccc gccaatggtc cgcttgcgga gggcgagtcc ggccccggcc ggatgctcga
    23701 accgcagcaa ctggtgcagg cgttgctggc gcctgctgga atgcatgttg catgaccgag
    23761 tagtgtcgat gacggtcgag gggtggttgc gtggaatcgt ctgaacgggc ggggggggcg
    23821 ttggccggcc tgcgggtcgt cgtgacggcc gggccgacct acgaggatat cgatccggta
    23881 cgcttcctcg gcaatcgcag ctccggcaag atgggcttcg cgatcgcgga agccgcgtcg
    23941 ctggcgggtg ccgacgtcac gctggtcgcg gggccggtgg cgctggcgac gcctgcgaaa
    24001 gtcgccctcc gggtggatgt gcgaagcgcg ctgcagatgc gcgatgcggt gctggaagcc
    24061 gtacgcgacg ccgacatctt cattgcgacc gcggcggtcg ccgactggcg ggtcgccgaa
    24121 tactcgccgc gcaagatcaa gaagggcacg ggcgcgccgg tgctggcgct tgccgaaaat
    24181 cccgacatcc tcgccgaagt ggccgcccgc gaggcgcgcc cgttcgtggt cggcttcgcc
    24241 gccgaaaccg atcggctcgc cgagcacgcg cgcgccaagc tcgtccgcaa gaagctggac
    24301 atgctggcgg cgaacctcgt cggcgagcac gtggggatcg gcgtcgacga caatgccgtc
    24361 gagcttttct ggccgggcgg cagcgaggcg ttgccgcgcg cctccaaggt cgaactcgcg
    24421 aaagcgctgg tggaccgcgt cgcccggcgt ttccacgcac ggagacgggc atgagggccg
    24481 gcatgcccgg cggcgaggtc gcggtgcacc tcaagcggct cgacgcgcgg ctcggcgaca
    24541 gcattccgct gcccgcatat gccaccgacg gcagcgccgc gatggacctg cgcgcggcgc
    24601 cggaagtcga catcgtgttg tcgcctggcg acagcgcgtt ggtgccgacg ggactggcga
    24661 tccacatcgc cgatcccggt tggtgcgcgc tgatccttcc gcgttcgggc ctcggccaca
    24721 aacacggcct ggtgctgggc aacctgaccg ggttgatcga tgccgattac caggggccgc
    24781 tgatgatttc ctgctggaac cgcgggaaac tgccctttac catccggccc ggggaccgca
    24841 ttgcccagct gatggtggtg ccggtggcgc gggtggcctg gcgtgttgtc gatgagttcg
    24901 ctgcgagtgg acgcggcgca ggggggttcg gctccacggg agtcgcgtga cgacatggca
    24961 gacaaatcgg cggtgaggaa atccccgaac gtgaaggctg ccgcgcgcgc ggtgcgtgcg
    25021 cgcctcagct tgcgtcgcgg gttggccgaa gtcgcggccg acatcgcctt ggcgctcggc
    25081 accctgctgc tgttgttcgg cttgttcatg ggctggcaga cgtggcagat cacccgccag
    25141 gcatcggcaa cccaggcgct ggcacgcggc aagcaggagg ccgcgcagaa gatcaccgcg
    25201 atcgtcgcgg acaaacgcgc ggaagtcgcg cgggcacttg cgcagccggc ggtagtggcg
    25261 gaactcgcga ccgacgctgg cccggagtcg ctggccgctg ccgcgaagca ggtccaggcc
    25321 gccatcgatg gcgcgcccaa agtgaccctc tacagcgcgc agttgaacga agtactgcac
    25381 ggaaacctgc aggacctcgg atatgcgcgc gcggcgcaac tgatgtccgc gctcagcgac
    25441 gatgaactcg gccccgccca gtcgccggtg gcgcgcatct tgagtttcgc cgggcccatc
    25501 gtcgggcccg atggcaatgt gctggcgtac gcgaccgtcg atttccgcga ttcgctgatg
    25561 cgcatcgcca tgagcacccc gcgaatcagc gccggccggc tggagttgcg ccagacgggc
    25621 aagtcgggcg acgtcctgct ggacagtgtc ggcgagcatg gtccttcgtt ggacgatgac
    25681 gtgggggtac gcgttcccca cagcctgttc gtcatcacct cggcgccgcc cgattactgg
    25741 ttgcccatca cccgctcgct cattcccgca ctggcgctcg ccgtggtcgc cttgtttctg
    25801 gggatcgcgt tgctgtggtg gcggccgcgc ctgcgcgtgc gcctcggcgg cgccgcatcg
    25861 gccgaggaac cggcgctggc ggacgtcatc gcggcggcac cccggccgcc ggcgcccacg
    25921 cccaagccgg tcaccgagcc ggagcaggcg gacacggccg cggcggcggc tgcggtccag
    25981 ctcgacaact cgctgcccga tcgcggcatc ttccgcgcct acgacatccg tggcgtcgtc
    26041 ggcaagacgc tgagcacggg cgtcgcgcgg cagctcggcc gcgcgatcgg cagcgccgtg
    26101 cgcgagaagg aactcgcgga gattgtcgtc gggcgcgatg gccgcctgtc gggacccgat
    26161 ctttccgacg ccctgatcga aggactgcgc gcggcggggg tggacgtgat cgacatcggc
    26221 gcggtgccga cgccggtcag ttacttcgcg gcgtaccagc tcaacaccgg ttcgtgcgtg
    26281 tcggtgaccg gcagccacaa tccgccggac tacaacggtt tcaaggtcgt gatcggcggc
    26341 gaaacgctgg ccgaggcggc gatccaggac ctgtaccagc gtatcgtcga gggacgcttg
    26401 gccgagggcg agggcaacct gcggcaatac gacattgccc ccgaatacgt cgagcgcatc
    26461 gcttcggaca tccagaccgg gcgctcgctg aaggtcgtgg tcgattgcgg caatggcatc
    26521 gccggcaata ccgcgccggc cgtggtgcac gcgatcggct gcgaaccggt cccgctgtat
    26581 tgcgacgtcg acggcacgtt ccccaaccac catcccgatc cgtccgaacc ggcgaacctc
    26641 tcggacctga tcctgacggt ccagaagacc ggcgccgacc tcggcctagc cttcgacggt
    26701 gatggcgacc gcctgggcgt cgtaaccaag gatggcgaga tcatctatcc cgatcgcgta
    26761 ttgatgctgt tcgcgcagga cgtgctggtc cgcaatccgg gcgcgacgat cctgtacgac
    26821 gtgaagtgca ccgggcacct gcagccgctg atcctgcagg ccggcggcag cccgctgatg
    26881 tggaagaccg gtcactcgct gatcaagtcc aagatgcggg aaaccggcgc gcagctcgcc
    26941 ggcgagatga gcgggcactt cttcttcc
//
LOCUS       DASSNH010000299        11822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1410221, whole genome shotgun sequence.
ACCESSION   DASSNH010000299 DASSNH010000000
VERSION     DASSNH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410221"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="lepA"
                     /locus_tag="VFK00_11150"
     CDS             <1..1176
                     /gene="lepA"
                     /locus_tag="VFK00_11150"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET7593546.1"
                     /translation="VVMLVRVMQGEIKPGDKLQVMSTGRSHQVDNVGVFTPKRKESSR
                     LGAGEVGWVNASIKDVHGAPVGDTLTLASDPAPKPLPGFQHMQPRVFAGLFPVASDDF
                     PALREALDKLRLNDAALFFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYDLDLVT
                     TAPTVVYQVLRSDGSVLDLDNPAKLPPANEVEEIREPIIVADILTPAEYVGNVITLCE
                     EKRGVQRSIQYLASQVRVSYELPLAEVVLDFFDRLKSVSRGYASMDYHFDRFEAGPFV
                     RVDVLINGDRVDALSLIVHRAQADRRGRDLVERMRELIPRQMFDVAIQAAVGAQIIAR
                     STVKALRKNVVAKCYGGDVSRKKKLLEKQKEGKKRMKQVGQVQIPQEAFLAVLRTDK"
     gene            1369..2334
                     /gene="lepB"
                     /locus_tag="VFK00_11155"
     CDS             1369..2334
                     /gene="lepB"
                     /locus_tag="VFK00_11155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7593547.1"
                     /translation="MKKDAWGTVVLVAIVAICLYIWYRTSDFALALVLITGAFFVIWF
                     VDWLFFARRRKARAAAEDQKPHEPVLVDYARSFFPVILAVLLFRTFLLEPFRIPSGSM
                     MPTLLVGDFVLVNKFAYGLRLPVTDTKIVGIGEPKRGDIVVFRYPGYTCHLPDGRVVR
                     GGDRACTTPVPQEDYIKRIIGLPGDTVAVRGEQVFIDGKPMPQNLIGPYRGTDKVSIR
                     MEENPMMAPVTLHEEDLGGVKHQILQFGYIEPYHCIGSGGTRLPEGGCQWVVPQGQYF
                     AMGDNRDDSEDSRYWGFVPEQNLAGKAFFIWFSLADWHRIGTVLD"
     gene            2524..2907
                     /locus_tag="VFK00_11160"
     CDS             2524..2907
                     /locus_tag="VFK00_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HET7593548.1"
                     /translation="MKRQSGITLIGFVIVLVLAAFFIYTAMKLVPAYIDYFNVAKAMN
                     TVASQTASSGMSPEEIRRQLDTQQLSQYFADEDIAEGNVHVQRTPNGGSSLKLSYDKK
                     IPWIYNIDFLLHFEKSVPLQAATPG"
     gene            2909..3565
                     /gene="rnc"
                     /locus_tag="VFK00_11165"
     CDS             2909..3565
                     /gene="rnc"
                     /locus_tag="VFK00_11165"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7593549.1"
                     /translation="MKLGYRFADEELLRLALTHRSAGRPHNERLEFLGDALVNLVVAE
                     MLFDARPRADEGELSRLRAQLVSEPALAERARDLDLGNELRLGSGELKSGGFRRASIL
                     ADAFEALVAAIYRDGGYAACRDWLRGVFAQPVADAGAPRKDPKTRLQEWLQGRGLPLP
                     RYELVAEHGEEHARRFDVVCVITQPRAARFDGHGGSRRSAEQRAAEAALEGLQGSEAP
                     "
     gene            3705..4598
                     /gene="era"
                     /locus_tag="VFK00_11170"
     CDS             3705..4598
                     /gene="era"
                     /locus_tag="VFK00_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7593550.1"
                     /translation="MSDPFRCGSVAIVGRPNVGKSTLLNALIDAHLSIVTPRAHTTRH
                     RILGIANPAGGQILYLDTPGLHREAKRAINRSLNRAVHAAVADADLAVQVVTAGQWDE
                     EDAAVYSAVAERGMPRLLAVNKIDKQRDKAKLLPFVEGLAREREWDEVYLVSAARRAG
                     LADLERGILARLPEGEARYDRDTFTDRSERFLAAELVREQLMRQLGEELPYATTVEIE
                     RFEDRKDGVAEIAAVVWVERDGQKAIVIGAGGRRAKAIGSAARKEMENLLGRRVFLKL
                     WVRVRAGWSDDDAALRQFGYE"
     gene            4687..5406
                     /gene="recO"
                     /locus_tag="VFK00_11175"
     CDS             4687..5406
                     /gene="recO"
                     /locus_tag="VFK00_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7593551.1"
                     /translation="MRITRQPAYVLHARAWRETSLLLEVFSRDFGRVGLVARGVRGAR
                     ARLPRSLLEPLQPLWLDWAGRGELATLAAVEAAGAPRTFRGDALLSAMYVNELLVRLT
                     ARNDPHAWLFDRYQELLEELPNTSSLAWSLRCFERDLLAAVGYALQLGVAADTGDPIE
                     AGASYDYLPESGAVAAADHPDGVRIRGSALLALAARECPDSRDLAALKRLMRNLIGRQ
                     VGSRGLQSWRVLAESFASDRP"
     gene            complement(5524..6243)
                     /locus_tag="VFK00_11180"
     CDS             complement(5524..6243)
                     /locus_tag="VFK00_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7593552.1"
                     /translation="MEATPFKVLLAEDDPVSRAFLREAAHACGAAVTDCADGAEALEL
                     ARADRWDLLVLDHHLPGMDGDAILSTLRADPAAASRAAPAIATTAARDVDAEALGSAG
                     FAEVLPKPMPIAILREALRRHGCGVDARLDDEDALRACGSATAVVRLRRLFAEQELPR
                     VQEELERVGDDLQSLRPTLHRLRASCGFCGARTLAEASAALHYAIVDPEVGSAGAALD
                     AFRVAVAETRAALHAQLDAVT"
     gene            6361..8346
                     /locus_tag="VFK00_11185"
     CDS             6361..8346
                     /locus_tag="VFK00_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593553.1"
                     /translation="MLVCACAQAALHLQADSLELPGASLVDVTMAIAPGSDGMPQLHL
                     DAAKVAVPALGWHDVVLSLDGQPQRAGSGAWKFVGHVTTRHAPGDAFADADLTVLLDP
                     AAGTLEVDVGQGQGNLHALMPLDQASHVQMTLSGLPLAWLRGVLASAWPEGRLAGGTV
                     GGDVAMDLAAGGARISGRVEVAGANLDSKAGNIAMQDLAADGSFRIESGSASASIMFD
                     GELRGGQMLFGPLYATLPPHPANVHVAALLGATGIAIKSLDYDDHDALRVSGSIGFDR
                     KGNLDKLDLDRFSASLPQAYDRYGTTFVESITGFSQVDTSGSLAGAIDLGSDGLEALD
                     LTVRGVSIDSHGAGLGLSGLDGKVDWRAQASRPATTLSWDGLSMYRIPFGPAKLVLED
                     QAGALTLQQPVAIDMLGGSFQLNRFAWQPGAEKSRLSAGFAVTGVDLPNLCAAFGWPS
                     FGGTLGGAVPDLTWRGDDLVFDGGLSLDVFGGSVSVTNLELQHLFGAQPRMAADVDLR
                     QLDLGQVTSVFDFGQITGRLDGDISGLQLVDWKPVAFQAKLATDRGGKISQHAIKSLT
                     EVGGGGIAGGLQSIALRLFKTFGYSRIGLSCVLAHGVCTMAGITPDPNPDGDGYTIVE
                     GSGLPHITVIGHQRKVDWATLVNRLEEATRSGGPVIR"
     gene            8447..9079
                     /locus_tag="VFK00_11190"
     CDS             8447..9079
                     /locus_tag="VFK00_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HET7593554.1"
                     /translation="MRKTAFPLTLALALLAGCVTINVYFPAAAAQKAADKVIGNIIGP
                     EGAASPAPAGSTQPPASDDDSSPGGFGGEPLAMRVLDALVPAANAAESQPDLDIHTPA
                     IDATMARMRSRYKSTLKALLDSGAIGFTHNGDVAIHDARKIPLPERAQANRAVAAENA
                     DRADLYKEIAAANGHTEWASRMRESFAKRWIAMAHPGWYYQDASGNWQKK"
     gene            9243..10187
                     /locus_tag="VFK00_11195"
     CDS             9243..10187
                     /locus_tag="VFK00_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7593555.1"
                     /translation="MRIGIVVDSTCDLPRKFIDDNQITILPITIRIDGDTFVDVRDPD
                     ATRHYFQGELGKRGHAAETEPLSVDAIRELFLGRLVLDYDAVFCLTVTSSRSPIFANA
                     TQASFAILSSYRDIRQGAGNQSPFMMRVIDTRSLFSGQGVSAVEATRMIAADAGAGQM
                     RERLSELSAQTYAYALPCDLHYLRARGRSKGDRSVGLLSATLGTALDIKPILQCFQGE
                     TRPVAKVRGFEQGAAMLFRHAAAAVERGLLVPALCVSYGGELAELEALPGYGELVEAC
                     AAAEVTLYQSVMSITGMVNVGTGAVTLGYAAASDDARF"
     gene            10223..11542
                     /gene="rlmD"
                     /locus_tag="VFK00_11200"
     CDS             10223..11542
                     /gene="rlmD"
                     /locus_tag="VFK00_11200"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7593556.1"
                     /translation="MKNPREHSFEATIDDLSHDGRGVAHVDGKTVFVADALPGEWVRA
                     RLARRHRHFDEARAEEILEASADRVAPRCPHYGVCGGCALQHLAPAAQIAAKQNVLAQ
                     NFARIGHVEPARWLEPLTADAWAYRRRARLSVKHVAKKGRTLVGFRERDPRFVADLAH
                     CDVLDPALGERLGELGAMLNTLNAAASIPQIEFSAGDEARVLVFRNLQPLSGADRGRL
                     VAFGREHDFAVMLQPGGTDSVQPLWPEHVDLRYALPEYGLELAFRPLDFVQVNARLNR
                     RMIDHALALLAPDPGDRVLDLFCGLGNFSLPIARRAASVSGVEGDAGLIERARANAAS
                     NGIANAEFHLGDLAGDVRGSAWARAEWDLMMLDPPRSGADELLKQLPGKSVRRVVYVS
                     CHPASLARDAGTLVREHGFRLDAAGVMDMFPHTAHVESIALFDRNAS"
BASE COUNT         1964 a         3899 c         4070 g         1889 t
ORIGIN      
        1 gtggtgatgc tggtgcgcgt gatgcagggc gaaatcaagc ccggcgacaa gctgcaggtg
       61 atgtcgaccg gccgcagcca ccaggtggac aacgtcggcg tattcacgcc caagcgcaag
      121 gaatcgtcgc ggctcggcgc cggcgaggtg ggctgggtca atgcctcgat caaggacgtg
      181 cacggggcgc cggtcggcga caccctgacc ctcgcctccg atccggcgcc gaagccgctg
      241 ccgggcttcc agcacatgca gccgcgcgtg ttcgccggcc tcttcccggt cgcctccgac
      301 gatttcccgg cactgcgcga ggcgctggac aagttgcgcc tgaacgacgc ggcgctgttc
      361 ttcgaacccg agtcgtccga ggcgatgggc ttcggtttcc gctgcggctt cctgggaatg
      421 ctgcacatgg agatcgtgca ggaacgtctg gagcgcgagt acgacctcga tctcgtcacc
      481 accgcgccga cggtcgtgta ccaggtgctg cgcagcgacg gcagcgtgct ggacctggac
      541 aacccggcca agctgccacc cgccaacgag gtcgaggaga tccgcgagcc gatcatcgtc
      601 gccgacatcc tgacgccagc ggagtacgtc ggcaacgtca tcaccctgtg cgaggaaaag
      661 cgcggcgtgc agcgctcgat ccaatacctc gcgagccagg tgcgggtcag ttacgaattg
      721 cccctggccg aagtggtgct cgacttcttc gaccgcctga aatcggtgag ccgcggctac
      781 gcctcgatgg actaccactt cgaccgcttc gaggcgggtc cgttcgtgcg ggtggacgtg
      841 ctgatcaacg gcgaccgggt ggacgcgctt tccctgatcg tgcatcgtgc gcaggccgac
      901 cggcgcggcc gcgacctggt ggaacgaatg cgcgagctga ttccgcggca aatgttcgac
      961 gtggcgatcc aggccgcggt cggcgcgcag atcatcgcgc gctccacggt caaggcgctg
     1021 cgcaagaacg tggtggccaa gtgctacggc ggcgacgttt cgcgcaagaa gaagctgctg
     1081 gagaagcaga aggaaggcaa gaaacgcatg aaacaagtcg gccaggtgca gattccgcag
     1141 gaagcgttcc tggcggtgct gcggaccgac aaatgaagca gggaacaggg aacggggagc
     1201 agggctcgga gccgaatacc cccttccaaa ggaagggggt cgatgccgcc cgcaagggcg
     1261 gcatccgggg ggatgccacc ggagacggag tcgccccaat gaccaaagac actggatgcc
     1321 ggcccgcgcc ggcatgacga tggctggcgc gcatacaagg atcacgcagt gaagaaagat
     1381 gcctggggta ccgtggtgct ggtggcgatc gtcgccatct gcctgtacat ctggtacagg
     1441 acatcggatt tcgcgctggc gctggtactg atcaccggcg cgttcttcgt catctggttc
     1501 gtcgactggc tgttctttgc caggcgccgg aaggcgcgcg cggccgccga agaccagaag
     1561 ccccacgagc cggtactcgt cgattacgcg cgctcgttct tccccgtgat cctcgcggtg
     1621 ctgctgttcc gcaccttcct gctggagccg ttccggattc cctcgggctc gatgatgccg
     1681 accctgctgg tgggcgactt cgtgctggtg aacaagttcg cctacggctt gcggctcccg
     1741 gtcaccgaca ccaagatcgt cggtatcggc gagccgaagc gcggggacat cgtggtgttc
     1801 cgctatccgg gctacacctg ccatctcccc gatggccggg tggtgcgcgg gggcgatcgc
     1861 gcctgtacca ccccggtgcc gcaggaggat tacatcaagc gcatcatcgg gctgccgggc
     1921 gacaccgtcg cggtccgcgg cgagcaggtc ttcatcgatg gcaagccgat gccgcagaac
     1981 ctgatcggcc cgtaccgcgg caccgacaag gtcagcatcc ggatggaaga aaatccgatg
     2041 atggcgccgg tgacgctgca cgaggaggac cttggcggcg tcaagcacca gatcctgcag
     2101 ttcggttaca tcgagcccta ccactgcatc ggttcgggcg gcacccggct gccggagggc
     2161 gggtgccagt gggtggtgcc gcaagggcag tacttcgcga tgggcgacaa ccgcgacgac
     2221 agcgaggaca gccgttactg gggcttcgtg ccggagcaga accttgccgg caaggccttc
     2281 ttcatctggt tcagcctcgc agactggcac cgcatcggta ccgtcctgga ttgagggagt
     2341 cgtgggaatg ccactgccgg tggacgccgg tcacgatttg acgcgccggc atgtggcgcg
     2401 taccgcaccg gcacccgtct tgcattaagc tctgctggcc cggcaggatg ccgcggccgg
     2461 cgcgcgttgt ggcgcggccg ggaagcgggt ggaatctcaa cagcaggtca agggggcgac
     2521 tccatgaaac gtcaatcggg cattaccctc atcgggttcg tgattgtcct ggtccttgcc
     2581 gcgttcttca tctacacggc gatgaaactg gtaccggcct acatcgacta cttcaacgtg
     2641 gcgaaggcga tgaataccgt tgcctcgcag acggcgtcga gcgggatgag tcccgaggag
     2701 atccgccggc aactggatac gcaacagttg tcccagtact tcgccgacga ggacatcgcc
     2761 gagggcaacg tccacgtgca aaggacgccg aacggcggtt cgagcctgaa gctgagctac
     2821 gacaagaaga tcccctggat ctacaacatc gacttcctgc tgcacttcga gaagtcggtg
     2881 cccctgcagg cggcgacgcc gggataacgt gaagctcggc taccgcttcg ccgacgagga
     2941 attgctccgc ctcgcactca cccaccgcag cgccgggcgt ccccacaacg agcggctgga
     3001 attcctcggc gacgcgctgg tcaacctggt ggtcgcggag atgcttttcg acgcgcgtcc
     3061 acgtgccgac gaaggcgagc tctcgcgcct gcgtgcgcaa ctggtgagcg aacccgcact
     3121 cgcggaacgc gcacgcgacc tggatctcgg caacgaactc cggctaggtt ccggggagct
     3181 gaagagcggc ggattccggc gcgcttcgat cctcgccgac gcgttcgagg cgttggtggc
     3241 cgcgatctac cgcgatggcg gctacgcagc ctgccgggat tggctgcgcg gcgtattcgc
     3301 ccagccggtg gccgatgccg gggcaccgcg gaaggatccc aagacccgcc tccaggaatg
     3361 gttgcagggc cgcggcctgc cgctgccccg gtacgaattg gtcgccgagc acggcgagga
     3421 acacgcaagg cgcttcgacg tggtgtgcgt gatcacgcaa ccgcgggccg ctcgcttcga
     3481 cggccacggt ggcagccggc gctcggccga gcaacgagcg gcggaagccg cattggaagg
     3541 tctccaagga tccgaagcac cctgacggat ccgcagcgct cgctcaggaa agacgtccct
     3601 ggcgtcgagg aaagcggatt gcacggacag ctccgcagct atgcttcttc cttcatccga
     3661 atgaatccgc gtccaatgct cttttcgatg gagcgtagtt ttccgtgagc gacccattcc
     3721 gctgcggcag cgtagcgatc gtcgggcgtc ccaacgtcgg caagtcgacg ttgttgaacg
     3781 cgctgatcga cgcgcacctt tcgatcgtga cgccgcgcgc ccacaccacc cggcatcgga
     3841 tcctcggcat cgccaacccc gcaggcgggc agatcctcta tctcgatacg cccgggctgc
     3901 accgcgaagc caagcgcgcc atcaaccgca gcctgaaccg cgccgtgcat gcagccgtcg
     3961 cggacgccga tctcgcggtg caagtggtta ccgccggaca gtgggacgag gaagacgccg
     4021 ccgtgtactc ggccgtggcg gaacgcggca tgccgcgcct gctggccgtc aacaagatcg
     4081 acaagcaacg tgacaaggca aagttgctgc cgttcgtgga aggcctggcg cgcgaacggg
     4141 agtgggacga ggtgtacctc gtcagcgccg cgcgccgcgc gggcctggcc gatctcgaac
     4201 gtggcatcct ggcgcggctg cccgagggtg aagcccgcta cgaccgcgac accttcaccg
     4261 accgcagcga acgtttcctc gccgccgagc tggtgcgcga acaattgatg cgccaactcg
     4321 gcgaagagtt gccgtatgcg accaccgtcg agatcgaacg cttcgaggac cgcaaggatg
     4381 gcgtcgccga aatcgcggcg gtggtctggg tggagcgcga tggccagaag gcgatcgtga
     4441 tcggcgccgg cggccgccgc gccaaggcga tcggcagcgc cgccaggaag gaaatggaga
     4501 acctgctcgg caggcgagta ttcctgaagc tgtgggtgcg cgtgcgcgcc ggctggtccg
     4561 acgacgatgc ggcgctgagg cagttcgggt atgaataaga gcggggaaca gggaacaggg
     4621 aacagggacg ggcgccggcg tgtgccttcc ccgttccccg ttccccgttc ccgctccccg
     4681 cagtaaatgc gcatcacccg ccaacccgcc tacgtgctgc acgcccgcgc gtggcgcgag
     4741 accagcctgc ttctcgaggt attcagccgc gacttcgggc gggttggact ggtggcgcgc
     4801 ggcgtgcggg gcgcccgcgc gcgcttgccc cgatcactgc tggagccgtt gcagccgttg
     4861 tggctggact gggcagggcg tggcgaactc gcgacgctgg cggcggtgga agcggccggc
     4921 gcgccccgaa cgtttcgtgg cgatgcgctg ctgtcggcga tgtacgtcaa cgagctgctg
     4981 gtgcggctca ccgcgcgcaa cgatccgcat gcctggctgt tcgatcgtta tcaggagctg
     5041 ctcgaggaac tgccgaacac atcgtcgctt gcatggagcc tgcgctgctt cgagcgcgac
     5101 ctgctggcgg cggtcggcta tgcattgcag ctcggggtgg cggcagatac aggcgatccc
     5161 atcgaggccg gcgcatcgta cgactacctt cccgagtccg gtgcggtggc ggccgccgat
     5221 catcccgacg gggtgcggat acgcggttcc gcgctgctgg cgctggcagc gcgcgagtgc
     5281 cccgactccc gggatctggc cgcgttgaag cgcctgatgc gcaacctgat cgggcggcag
     5341 gtggggtcgc gcgggctgca atcgtggcgg gtgctggccg agagttttgc aagcgatcgc
     5401 ccgtgatcgc tgcttgccaa gtgttcggga ggcgccgctc gccccaggcg tggacaccag
     5461 aacggcgttc cgtggcctga ctttccgcaa cagcggcttc gagcaggagt cgatcctcag
     5521 ttgctaggtc acggcgtcga gctgcgcgtg cagcgcggcg cgcgtctccg cgaccgcgac
     5581 ccggaaggca tcgagcgcgg ctccggcgga accgacttcg ggatccacga tcgcgtagtg
     5641 caacgccgcg ctcgcctcgg cgagcgtcct ggcgccgcag aagccgcagg atgcccgcag
     5701 ccggtggagg gttggccgca gcgactggag gtcgtcgccg acgcgctcga gttcttcctg
     5761 gacccttggc aactcctgct cggcgaacag tcgccgcaac cggaccacgg ccgtggccga
     5821 cccgcatgcg cgcaaggcgt cctcgtcgtc gagccgggcg tccacgccgc agccgtggcg
     5881 ccgcagcgcc tcgcgcagga tcgcgatcgg catcggcttg ggcaatacct ccgcgaagcc
     5941 cgccgacccg agcgcttcgg catcgacatc gcgcgcggcg gtcgtggcga tcgccggcgc
     6001 cgcacgcgag gccgcggcgg ggtcggcccg cagggttgag aggatggcgt cgccatccat
     6061 gcccggaagg tggtggtcga ggaccagcag gtcccatcga tccgcgcgcg ccagctcgag
     6121 cgcctccgcg ccgtccgcgc agtcggtgac ggccgcgccg caggcgtgcg cggcttcccg
     6181 cagaaaggcg cggctgaccg ggtcgtcctc ggccaggagc accttgaatg gagtggcttc
     6241 catgcgaaac atgccctcgt tggcatccgt tcggatgcgg gctggcacaa tcggcgtcac
     6301 tgttaccgga aaagggtggt cgtggcaagt cggcgccagt acatcgtggc gggatgcctg
     6361 atgctcgttt gcgcgtgtgc ccaggccgcg ctgcacctcc aggccgactc gctggaatta
     6421 ccgggcgcca gcctggtcga cgtcacgatg gcgatcgcgc cggggtccga tggcatgccg
     6481 caactgcacc tggacgccgc caaggtggcg gttccggcgc tgggctggca cgacgtggtg
     6541 ctgagcctgg acggacaacc gcaaagggcc ggcagcggcg cgtggaaatt cgtcggtcac
     6601 gtcacgacgc ggcacgcgcc gggcgacgcc ttcgccgatg ccgacctcac ggtgctgttg
     6661 gacccggccg ccggcaccct cgaggtcgat gtggggcagg gccagggcaa cctgcatgca
     6721 ctgatgccac tggaccaggc gagccacgtc cagatgaccc tcagcgggct gccgctcgcc
     6781 tggttgcgcg gcgtgctggc gtcggcgtgg ccggaagggc gcctcgccgg cggcaccgtc
     6841 ggtggcgacg tggcgatgga cctggccgcc ggaggcgcgc ggatttccgg tcgcgtcgag
     6901 gtcgccggcg ccaacctcga cagcaaggcc ggcaacatcg cgatgcagga tctcgctgcc
     6961 gacggcagct tccgcatcga aagcgggtcc gcatcggcca gcatcatgtt cgacggcgaa
     7021 ctgcgtggcg gccagatgct gttcgggccg ctgtacgcga ctcttccgcc gcatccggcc
     7081 aacgtccacg tcgccgcgct gctcggagcg acgggcatcg cgatcaagtc gctcgactac
     7141 gacgaccacg atgcgctgcg cgtctcgggc agcatcggct tcgaccgcaa gggcaacctc
     7201 gacaagctgg acctcgaccg gttctccgcg agcctcccgc aagcgtacga tcgctacgga
     7261 accacgttcg tcgaatcgat caccgggttt tcgcaggtcg acacctcggg cagcctcgcg
     7321 ggtgcaatcg accttggcag cgacggactc gaggcgctgg acctgaccgt gcggggtgtt
     7381 tccatcgaca gccatggcgc cggcctcggc ctgtccggtc tcgacggcaa ggtcgattgg
     7441 cgtgcgcagg cgtcgcgtcc cgccacgacc ctgtcctggg acgggttgtc gatgtaccgg
     7501 atcccgttcg gtccggcgaa actggtcctc gaggaccagg cgggtgcgct gaccctgcaa
     7561 cagccggtcg ccatcgacat gctgggcgga tcgttccagc tcaaccggtt cgcctggcag
     7621 cccggcgccg aaaagtcgcg cctttcggcg ggcttcgcgg tgaccggcgt ggatctgccg
     7681 aacttgtgcg cggcgttcgg ctggccctcg ttcggcggca cgctcggcgg cgccgtgccg
     7741 gacctcacct ggcgcggcga cgacctggta ttcgatggcg ggctgtcgct cgacgtcttc
     7801 ggcggctcgg tgagcgtgac caacctggaa ttgcagcacc tgttcggcgc ccagccgcgg
     7861 atggccgccg acgtcgacct gcggcaactc gacctcggcc aagtcaccag cgtgttcgat
     7921 ttcggccaga tcaccggccg cctggacggc gacatcagtg gactgcaact ggtggactgg
     7981 aagccggtcg cgttccaggc gaagctcgcc accgaccgcg gcggcaagat cagccagcac
     8041 gccatcaaga gcctgaccga ggtcggtggc ggcggcatcg ccgggggcct gcagagcatc
     8101 gcgctccgcc tgttcaagac cttcggttat tcgcggatcg gcttgagctg cgtgctcgcc
     8161 cacggcgtgt gcacgatggc aggcatcacg ccagacccga atcccgatgg cgacggttac
     8221 acgatcgtgg aaggcagcgg ccttccgcac atcaccgtga tcggccacca gcggaaggtc
     8281 gactgggcga ccctggtcaa ccgcctcgag gaggccacca ggagcggcgg tccggtgatc
     8341 aggtgagcca ccgggggcgc ggacggctgc gtgtacactt tcggggacgg cgcaggcatg
     8401 aggcctgcgg caagtcgccg cagattccga tcgatggagg ttcgtgatgc gcaagacagc
     8461 tttcccgctg acgctcgcct tggcgctgct tgctggctgc gtcaccatca acgtctattt
     8521 cccggccgcg gccgcgcaga aggcggccga caaggtcatc ggcaacatca tcggtcccga
     8581 gggagcggcc tcgccggcgc cggccggcag cacgcagcct ccggcttcgg acgatgattc
     8641 gtcgcccggc gggttcggcg gtgaaccgct ggccatgcgg gtgctggatg cgctggtccc
     8701 ggccgccaac gccgcggaat cgcagcccga cctcgacatc cacacgccgg ccatcgacgc
     8761 gaccatggcg cgcatgcgca gccgctacaa gtcgacgctc aaggcgctgc tcgactccgg
     8821 cgcgatcggg ttcacccaca acggcgacgt cgccatccac gatgcccgga agatcccgct
     8881 gcccgagcgc gcgcaggcaa accgggccgt cgccgccgag aacgcggacc gcgcggacct
     8941 gtacaaggag atcgccgccg ccaacggcca tacggaatgg gcgtcacgga tgcgcgagtc
     9001 gttcgccaag cgctggatcg cgatggcaca cccgggatgg tactaccagg atgcttcggg
     9061 gaactggcag aagaaatgac gacaggatgt cgtcatcccg gccaacacag gaaaaatgtg
     9121 cgttgagccg gaatccagaa tgacatgtgg tgatcccggc cagcacacgg acgtaccctg
     9181 agccgcggtc gtcggaaaac agacatcgcg cggcgcgaag actttagggg aggaatacgc
     9241 caatgcgcat cggcatcgtc gtcgactcga cttgcgatct accgcgcaag ttcatcgacg
     9301 acaaccagat cacgatcctg ccgatcacga tccgcatcga cggcgacacg ttcgtcgatg
     9361 tgcgtgatcc ggacgccacc cggcactatt tccagggcga actcggcaag cgcggacatg
     9421 cggcggaaac cgagccgttg tcggtcgacg cgatccgcga gctgttcctg gggcggctgg
     9481 tgctcgacta cgacgcggtg ttctgcctca cggtgacgtc cagtcgcagc ccgatcttcg
     9541 cgaacgcgac ccaggccagt ttcgcgatcc tcagcagcta tcgggacatc cgccagggcg
     9601 ccggcaacca gtcgcctttc atgatgcggg tgatcgatac ccgcagcctg ttctcgggcc
     9661 agggcgtgag cgcggtggaa gccacccgga tgatcgccgc ggacgctggc gccgggcaaa
     9721 tgcgcgaacg gctgagcgag ctttccgcgc agacctatgc gtacgccctg ccgtgcgacc
     9781 tgcattacct gcgggcgcgc ggacgcagca agggtgaccg cagcgtcggc ctgctcagcg
     9841 cgacactcgg caccgcgctg gacatcaagc cgatcctgca gtgcttccag ggtgaaaccc
     9901 ggcccgtcgc gaaggtacgc ggattcgagc agggcgccgc gatgctgttc cgccatgccg
     9961 ccgcagcggt cgagcgtgga ctgctggtgc cggcgttgtg cgtcagctac ggcggtgaac
    10021 tcgccgaact cgaagcgttg ccggggtacg gcgaactggt cgaagcctgt gccgcggcgg
    10081 aggtcacgct gtaccagagc gtgatgagca tcaccggcat ggtcaacgtc ggtaccggtg
    10141 cggtcaccct gggctacgcc gcggcttcgg acgacgcacg tttctgaacc ggcgtgcttg
    10201 acgcgtctgg caagatgcgc ggatgaaaaa tccgcgcgaa cattccttcg aagccacgat
    10261 cgacgacctg tcgcacgacg ggcgcggcgt cgcccatgtc gatggcaaga ccgtgttcgt
    10321 ggccgacgca ttgccgggcg aatgggtgcg cgcgcgcctg gcccgccggc atcgccattt
    10381 cgacgaggca cgcgccgagg aaatcctcga agcctccgca gatcgcgtgg cgccgcgctg
    10441 cccgcactac ggcgtctgcg gtggctgcgc attgcaacac cttgcgccag ccgcgcaaat
    10501 cgccgccaag cagaacgtgc ttgcgcagaa ttttgcccgg atcggccacg tcgaaccggc
    10561 ccgctggctc gagccgctca cggccgacgc ctgggcctac cggcggcggg cacggctgtc
    10621 ggtcaaacat gtcgcgaaga agggcaggac gctggtgggt tttcgtgagc gcgatccgcg
    10681 cttcgtcgcg gacctcgcgc attgcgacgt gctcgatccg gcgcttggcg agcggctggg
    10741 cgaactcggc gcgatgttga acacgctgaa tgccgcggcc tccattccgc agatcgaatt
    10801 ctcggccggg gacgaggcgc gggtgctggt gttccgcaac ctgcagccgc tgtccggggc
    10861 ggaccggggc aggctcgtgg cgttcggccg cgagcacgat tttgcggtga tgctgcaacc
    10921 cggcggcacg gacagcgtgc agccgctgtg gccggaacac gtcgacctgc gctatgcatt
    10981 gccggaatac ggtctcgagc tcgcgttccg cccgctggat ttcgtgcagg tcaatgcacg
    11041 cctcaaccgc cggatgatcg accatgcgct cgcgttgctc gcgccggatc ccggggaccg
    11101 ggtgctggac ctgttctgtg gcctcggcaa cttcagcctg ccgatcgcgc gccgcgcggc
    11161 ttcggtttcc ggcgtcgaag gcgatgccgg cctcatcgag cgggcgcgcg cgaatgcggc
    11221 gtcgaacggc atcgccaacg ccgagttcca cctgggagac ctcgccggcg acgtgcgagg
    11281 cagcgcatgg gcacgcgccg agtgggacct catgatgctg gatccgccgc gcagcggcgc
    11341 cgacgaactg ctgaagcaac tgccaggcaa gtcggtgcga cgcgtggtgt acgtttcctg
    11401 ccatccggcg tcattggcgc gcgacgccgg cacactggtg cgcgaacacg gcttccgcct
    11461 cgacgccgcc ggcgtgatgg acatgttccc gcacaccgcg cacgtggagt cgatcgcgtt
    11521 gttcgatcgg aatgcttctt gacacggatg aacgcggata aatacggatg aaacggagct
    11581 gtcatcccgc tttgatccgc gttcatccgc gtcgagggct tccagccggc gatatcccgc
    11641 gcctcgctcg cgatgccgta tgatcgtgcg tttgacggca cacgacggga tcgttgcgca
    11701 tgctgatcat cgggatcgaa ggcaccgaac tgacgaacgg cgatcgcgtg cggctggctg
    11761 cgccggaggt cagcggcgcg atcctgttca cccgcaatta ccgggaccac gaccagctcg
    11821 cc
//
LOCUS       DASSNH010000300         6610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1354353, whole genome shotgun sequence.
ACCESSION   DASSNH010000300 DASSNH010000000
VERSION     DASSNH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354353"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFK00_11205"
     CDS             complement(<1..1031)
                     /locus_tag="VFK00_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7593557.1"
                     /translation="MADPRPWLANYPPGVPAEIDTSAYASIVAMFDEACARHPQRPAY
                     SSFGKTLTYAELDRASQRFAAFMAGEWRLGKGDRLAIMLPNTLQYPIALFGALRAGLT
                     VVNVNPLYTARELEHQLADSGATAILVLENFAATLAKAIGATRVEHVIVTSLGDQLGF
                     PKGLLVSFAVKHVKKMVPAWSLPGHVRFNDVLARGAGMPVPQVELGQDDLAFLQYTGG
                     TTGVAKGAMLTHGNMVANTLQLATWVGDLFTRGEEQIITALPLYHIFSLTVNALFMTY
                     FGGENVLITNPRDIPAFIKTLKQSRWSAITGVNTLFNALVHHPDFSSIDFSRVKFSLA
                     GGMAVQRAVA"
     gene            1201..1623
                     /locus_tag="VFK00_11210"
     CDS             1201..1623
                     /locus_tag="VFK00_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593558.1"
                     /translation="MALLLAATLLAGCHRAPAEQQIHDAIEAAATAARANDTSGVLDV
                     VSDDFTGNEGDLDQRGLRRLLAVRAFRQDKTGILIGPITFDRKGDRVIAHFNLVLTGG
                     RQGDLLPDQSAVYAMTTAWRREGGEWRCYNATWTSAGR"
     gene            complement(1782..4535)
                     /gene="acnA"
                     /locus_tag="VFK00_11215"
     CDS             complement(1782..4535)
                     /gene="acnA"
                     /locus_tag="VFK00_11215"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET7593559.1"
                     /translation="MQDSFGIRDILEVDGKSYQIASLAKLGEQFDIRRLPYSIKVLLE
                     NLLRNEDGSEVTRAQIEAVAQWDPKAVPDKEISFLPARVVLQDFTGVPCVVDLAAMRD
                     AMVALGGDPKKINPQSPCELVIDHSVQVDVFGEADALVRNAEIEFQRNHERYAFLRWG
                     QKALEDFRVVPPSTGIVHQVNLEYLSRVVMTRDIDGKTWAFPDTLFGTDSHTTMVNGL
                     GVLGWGVGGIEAEAAMLGQGSSMLIPQVVGFRFTGKLPEGATATDLVLTVTQMLRKQG
                     VVGKFVEFFGPGLQHLPLADRATLGNMSPEYGATCAICPIDAESLNYLRLSGRDEKHV
                     KLIETYARAQGLWHDANAPGAEFSTVLELDLASVVPSLAGPKRPQDRVLLADMRKSYK
                     DTMQVLGSRRGPPQWDKQKERFLNEGGSTAVGRTESQEEFSPDVCPHGDHCELEDGSV
                     VIAAITSCTNTSNPAVMIGAGLLARKAAERGLKPQPWVKTSLAPGSKVVTDYLEKTGL
                     MEDLQKLGFYLVGYGCTTCIGNSGPLPEDVSKKVTENDFAVAAVLSGNRNFEGRIHSE
                     VKMNYLASPPLVVAFAIAGTIDIDLTRQPLGKGKDGKDVYLKDVWPTNKEIADLVASS
                     VNAGMFSKTYGEVFKGSKEWNAVEVPEGDQYAWDDKSTYIKNPPYFEGMAMELPTHGD
                     IEGAYCIGLFGDSITTDHISPAGAIKKDSPAGKYLMERGVEPKNFNSYGSRRGNDDVM
                     VRGTFANIRIRNRLLDNVEGGYTMHVPSGEQMTIYDAAMRYQKEGESLIVIAGKEYGT
                     GSSRDWAAKGTLLLGIEAVIAESFERIHRSNLVGMGVLPLQFKEGANAESLGLTGKET
                     YAITGLDNGDSRTAHVVATSKDGKKTEFDVHVMLLTPKERDFYKHGGILQYVLRDLAK
                     KAA"
     gene            4729..6537
                     /locus_tag="VFK00_11220"
     CDS             4729..6537
                     /locus_tag="VFK00_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7593560.1"
                     /translation="MTSPVRLFLRIYEKWKRDTLYEGDFRPSISILKLSFIALFIGGA
                     AAAIAWILYRLIGLVTHLAFAGEWDFTFTSPAELHAPWWVIILAPVVGGVIIGFMARY
                     GTDRIRGHGIPEALEAILFRRSKMMAKVAILKPLSAAVAIGTGGPFGAEGPIIMTGGA
                     VGSLIGQVFSLTSIERRTLLVAGACAGMSATFATPIAATLVGVELLLFEMRPRSTIPV
                     AIACFVAAMIRPFLIGPGPLFPIPIQFHLDIAELGMAAVSGLLAGLLATAVTFLVYKV
                     EDGYRKLPIHWMWWPAIGALVVGIGGVIDPQTLGVGYDIIYQLLTGKIVGTAIIVFMI
                     VKAIIWIVYLSSGTSGGTLAPLLALGAGLGGLEALFFPGPYSLLWPLLGMGAMLAAVM
                     RMPFTSILFALELTGNSSMLPALLITCTTAYGASVFLMRRSILTEKIARHGLNIFRPY
                     TLNRLEYMSVKEVMTTDVEAVRADATVRDLRDRYFGVDQRHRAYPVVDAEGVLLGIVD
                     RDRLRGWLENDEAGAARLSELMSGDPVAAFPEESCQSIAIRMAVEKRDRIPVVSRDGH
                     RLLGIVTRYDLLTPYRHTHDEELLRERVFRGHARTP"
BASE COUNT         1043 a         2158 c         2174 g         1235 t
ORIGIN      
        1 gccaccgcgc gctgcaccgc catgccgccg gccagcgaga acttcaccct cgaaaagtcg
       61 atcgacgaga agtcgggatg gtggaccagc gcgttgaaca gcgtattgac gccggtgatc
      121 gcgctccagc ggctttgctt cagcgtcttg atgaaggcgg ggatgtcgcg tggattggtg
      181 atgagcacgt tctcgccgcc aaagtacgtc atgaacagcg cgttcaccgt gagcgagaag
      241 atgtggtaca gcggcagcgc cgtgatgatc tgttcttcgc cgcgcgtgaa caggtcgccc
      301 acccaggtcg cgagttgcaa ggtattggcg accatgttgc cgtgggtgag catcgcgccc
      361 ttggcgacgc cggtggtgcc gccggtgtat tgcaggaagg cgaggtcatc ctggcccagc
      421 tcgacctgcg gcaccggcat gccggcgccc ctggccagga cgtcgttgaa acgcacatgg
      481 cccggcagcg accaggccgg caccatcttc ttgacgtgct tgaccgcgaa gctgaccagc
      541 aggcccttgg gaaagccgag ctggtcgccg agcgaggtca cgatcacgtg ctcgacacgc
      601 gtggcgccga tcgccttggc gagcgtcgcg gcgaagttct ccagcaccag gatcgcggtg
      661 gcgcccgaat cggcaagctg gtgttcgagc tcgcgcgcgg tgtacagcgg gttgacgttg
      721 accacggtca agcctgcccg cagggcgccg aacagcgcga tcgggtattg cagcgtgttg
      781 gggagcatga tcgcgaggcg gtcgcccttg ccgagcctcc actcgcctgc catgaaagcc
      841 gcgaaccgct ggctcgcccg gtcgagttcc gcgtaggtca gtgtcttgcc gaagctcgaa
      901 tacgccgggc gctgcggatg gcgcgcgcag gcttcgtcga acatggccac gatcgacgcg
      961 tacgcggacg tgtcgatctc ggcaggtacg ccgggcgggt agttcgccag ccagggacgc
     1021 ggatcggcca ttattccctc ccgtacgttc gccggttgcg cggattgact gcattggaca
     1081 cgataagcat gtgggcggca agctgcatcg ccgcaatgcc gaaaacggga tgtccgcgtg
     1141 atcgccactt ccatgcccgc cttgtccttc cgcgcacgaa tgccgcaggt cgctttcgcg
     1201 atggcgttgc tgctggcggc gacattgctc gccggctgcc atcgcgcgcc ggccgagcag
     1261 caaatccacg acgccatcga ggcggctgcc acggcagccc gtgccaacga cacctccgga
     1321 gtgctcgacg tcgtcagcga cgacttcacc ggcaacgaag gcgatctcga ccagcgcggc
     1381 ctgcgccgcc tgctggccgt gcgcgccttc cgtcaggaca agaccggcat cctgatcggc
     1441 ccgatcacct ttgaccgcaa gggcgatcgc gtgatcgcgc acttcaacct cgtgctgact
     1501 ggcggcaggc aaggcgacct gcttcccgac cagtccgcgg tctacgccat gaccaccgcc
     1561 tggcgccgcg agggcggcga gtggaggtgc tacaacgcca cgtggacaag cgccggccgt
     1621 tgacggcagg agcggtccag ggccgcgatc ggcgttcatg gctgcgccca tcgcgcgctc
     1681 ctacaagccg gggcgcttgc acgagcggcc catggccgcg gtcgtacgtt gcggtcacgc
     1741 ctgtggcgtg tgctcgctcc tgcaacagcc gtgttgccgg tctatgccgc cttcttggcc
     1801 aaatcgcgca gaacgtattg caggatgccg ccgtgcttgt agaagtcgcg ttccttcggc
     1861 gtcaggagca tcacgtgcac gtcgaactcg gtcttcttgc cgtccttcga cgtcgccacc
     1921 acgtgcgcgg tcctggagtc gccgttgtcg aggccggtga tcgcgtatgt ctccttgccg
     1981 gtcagaccga gagactcggc gttcgcgcct tccttgaatt gcagaggcag tacgcccatg
     2041 ccgacgaggt tcgagcggtg gatgcgctcg aagctttccg cgatcacggc ttcgatcccg
     2101 agcagcaggg tgcccttggc cgcccagtcg cgcgaggaac cggtgccgta ttccttgccg
     2161 gcgatcacga tcagcgactc gccctccttc tggtagcgca tcgcggcgtc gtaaatcgtc
     2221 atctgctcgc cgctcggcac gtgcatcgtg tagccgccct cgacgttgtc gagcagccgg
     2281 ttgcggatgc ggatgttggc gaaggtgccg cgcaccatca cgtcgtcgtt gccgcgccgt
     2341 gaaccgtagg aattgaagtt cttgggctcg acgccgcgtt ccatcaggta cttgccggcg
     2401 ggcgagtcct tcttgatcgc gccggccggc gagatgtggt cggtggtgat ggagtcgccg
     2461 aacaggccga tgcaatacgc gccttcgatg tcaccgtggg tcggcagctc catcgccatc
     2521 ccttcgaaat agggcgggtt cttgatgtag gtggacttgt cgtcccacgc gtactggtcg
     2581 ccctcgggca cctcgaccgc gttccattcc ttgctgccct tgaacacttc gccgtaggtc
     2641 ttggagaaca tgccggcgtt gacggagctg gcgacgaggt cggcgatttc cttgttggtc
     2701 ggccagacgt ccttgaggta gacgtccttg ccgtccttgc ccttgccgag cggctgccgg
     2761 gtcaggtcga tgtcaatggt gccggcgatc gcgaacgcca ccaccagcgg cggcgaggcg
     2821 aggtagttca tcttcacctc ggagtggatg cggccctcga agttgcggtt gccggagagc
     2881 accgcggcga ccgcgaaatc gttttcggtc accttcttgc tgacgtcttc gggcaatgga
     2941 ccggaattgc cgatgcaggt ggtgcagccg taaccgacca ggtagaagcc gagcttctgc
     3001 aggtcttcca tcaggcccgt cttctcgagg taatcggtga ccaccttcga gccgggcgca
     3061 agggacgtct tgacccacgg ttgcggcttg aggccgcgct cggcggcctt tcgcgccagc
     3121 agcccggcgc cgatcattac ggcggggttg gaggtgttgg tgcacgaggt gatcgcggcg
     3181 atcaccaccg agccgtcctc gagttcgcag tggtcgccgt gggggcaaac gtccggcgag
     3241 aattcctcct ggctttcggt gcggccgacc gcggtgctgc cgccctcatt gaggaatcgc
     3301 tccttctgct tgtcccattg cggagggccg cgtcgcgaac cgagcacctg catcgtgtcc
     3361 ttgtagctct tgcgcatgtc ggcgagcagc acccggtcct gcggccgctt gggacctgcg
     3421 agcgatggca ccacgctcgc aaggtcgagt tccagcactg tgctgaattc cgctcccggc
     3481 gcattggcgt cgtgccacag gccctgcgcc cgcgcatagg tctcgatcag cttgacgtgc
     3541 ttctcgtcgc gtcccgacag gcgcaggtag ttgagcgatt ccgcatcgat cgggcagatc
     3601 gcgcaggtcg cgccgtattc gggcgacatg ttgccgagcg tcgcgcgatc ggccagcggc
     3661 aggtgctgca agccgggacc gaagaactcg acgaacttgc cgaccacgcc ctgcttgcgc
     3721 agcatctggg tgaccgtcag cacgaggtcg gtggcggtcg cgccttcggg aagcttgccg
     3781 gtgaagcgga agccgaccac ctgcgggatc aacatcgacg atccctggcc gagcatcgcg
     3841 gcctcggcct cgatgccgcc gacgccccag ccgagcacgc cgaggccgtt gaccatcgtg
     3901 gtgtgcgaat cggtaccgaa cagggtgtcg gggaacgccc atgtcttgcc gtcgatgtcg
     3961 cgggtcatca ccacgcgcga caggtattcc aggttcacct ggtgcacgat gccggtggac
     4021 ggcggcacca cccggaaatc ttccagcgcc ttctggcccc agcgcaggaa tgcgtaacgt
     4081 tcatggttgc gctggaattc gatctcggcg ttgcggacca gcgcgtcggc ttccccgaac
     4141 acgtcgacct gcaccgaatg gtcgatcacc agttcgcacg gcgattgcgg attgatcttc
     4201 ttcggatcgc caccgagcgc caccatcgcg tcgcgcatcg ccgcaaggtc gaccacgcac
     4261 ggaacgccgg tgaagtcctg cagcaccacc cgcgcaggca ggaacgagat ttccttgtcc
     4321 ggcacggctt tcgggtccca ctgcgccacc gcttcgatct gcgcccgggt cacctcgctg
     4381 ccgtcttcgt tgcgcagcag gttttccagc agcaccttga tcgagtaggg caggcggcgg
     4441 atatcgaact gctcgccgag cttcgcgagg cttgcgatct ggtacgactt gccgtcgacc
     4501 tcgaggatgt cacggatccc gaaagaatct tgcatggtgc tctcctgatg cgcgcgtggc
     4561 gaccggtgtc gcgacgggtg gactccgaat tggacctccc attatcgcgc aaacgccgct
     4621 tccccgccgg aaacgcgggg agcaccactg gcgtcgaggg catgcggtgg ttacatttgg
     4681 tgcggttgcg ggtcgaatgc gcccgcccga gggggaaccc agccgctcat gacgtcaccg
     4741 gtccgcctgt tcctgcgcat ctacgaaaag tggaagcgcg acacgctcta cgagggcgac
     4801 ttccggccca gcatcagcat cctcaagctg tccttcatcg ccctgttcat cggtggcgcg
     4861 gcggccgcca tcgcgtggat cctgtaccgg ctcatcgggc tggtgacgca cctcgcgttc
     4921 gccggtgaat gggatttcac cttcacctcg ccggccgaat tgcacgcacc gtggtgggtg
     4981 atcatcctgg cgcccgtggt cggcggcgtg atcatcggct tcatggcgcg ctacggcacc
     5041 gaccggatcc gcggccacgg catccccgaa gcattggaag ccatcctttt ccgccgcagc
     5101 aagatgatgg ccaaggtcgc gatcctgaaa ccgctgtcgg cggcggtcgc gatcggcacc
     5161 ggcggtccgt tcggcgccga ggggccgatc atcatgaccg gcggcgccgt cggttcgctg
     5221 atcgggcagg tgttctcgct gacatcgatc gaaaggcgca cgctgctggt ggcgggcgcc
     5281 tgcgccggca tgtccgcgac cttcgcgacg ccgatcgccg cgacgctggt gggcgtggaa
     5341 ctgttgctgt tcgagatgcg tccgcgcagc accattccgg tggcgatcgc ctgcttcgtg
     5401 gccgccatga tccggccgtt cctgatcggg cccgggccgc tgttccccat cccgatccag
     5461 ttccacctcg acatcgccga actcggcatg gcggccgtca gcggcctgct ggccgggctg
     5521 ctggcgaccg ccgtgacgtt cctggtctac aaggtcgagg acgggtaccg gaaactcccg
     5581 atccactgga tgtggtggcc tgcgatcggc gcgctggtcg tggggatcgg cggcgtgatc
     5641 gatccacaga ccctcggcgt cggatacgac atcatctacc aactcctgac cggcaagatc
     5701 gtcggcaccg cgatcatcgt tttcatgatc gtcaaggcga tcatctggat cgtctatctg
     5761 agctctggaa cgtcgggcgg cacgctggcg ccgctgctgg cgctgggcgc agggcttggc
     5821 ggattggaag ccttgttctt ccctggcccg tattccttgc tgtggccgtt gctcgggatg
     5881 ggcgcgatgc tcgccgcggt aatgcgaatg ccgttcactt cgatcctgtt cgcgctggag
     5941 ctgacgggca attcgagcat gctgccggcg ttgctgatca cctgcacgac agcttatggg
     6001 gcgagcgtgt tcctgatgcg gcgatccatc ctgaccgaaa agatcgcccg ccacggcctc
     6061 aacatcttcc gcccgtacac tctcaatcgc ctggagtaca tgtcggtgaa ggaagtgatg
     6121 acgaccgacg tggaagccgt tcgcgcggac gcgacggtgc gggatctgcg ggaccggtac
     6181 ttcggtgtcg accagcggca tcgcgcgtat ccggttgtgg acgcggaggg cgtcctgctc
     6241 ggcatcgtcg atcgcgaccg cttgcgcggc tggctggaaa acgacgaagc cggggcggcg
     6301 cgcctttccg agctcatgag cggcgacccg gtggccgcat ttcccgaaga aagctgccag
     6361 agcatcgcca tccggatggc ggtggaaaag cgcgaccgca ttccggtggt cagccgcgac
     6421 ggccaccggc tgctgggcat cgtgacccgc tacgacctgc tgaccccgta ccggcacacc
     6481 cacgacgagg aattgctccg cgagcgcgtg ttccgcggac acgcgcggac tccctgacgc
     6541 gaacgggcga caacacccca tcgttgggct tccccggaac aagttccggg tcaacccaac
     6601 ctacaaaagc
//
LOCUS       DASSNH010000301        11545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_837001, whole genome shotgun sequence.
ACCESSION   DASSNH010000301 DASSNH010000000
VERSION     DASSNH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837001"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFK00_11225"
     CDS             <1..222
                     /locus_tag="VFK00_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593561.1"
                     /translation="SFGLLHAPNARTMAPAKADASTVRVNFMSLSPDGEPWIATEVAA
                     LLNSVPQNNASIQPFYEHITFSYRLPHWC"
     gene            complement(241..1416)
                     /locus_tag="VFK00_11230"
     CDS             complement(241..1416)
                     /locus_tag="VFK00_11230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7593562.1"
                     /translation="MSDVIIAAAKRTPIGAFLGQFTGFPTPQLGAAAIGAALQQSGVE
                     PGDVGEVLMGCVLPANLGQAPARQAALAAGLPPSTGCTTLNKVCGSGMKAIMLGRDLI
                     VAGSADVVVAGGMESMTNAPHMAAVRSGIRIGEGALVDHMMWDGLTNPYDGQSMGTFA
                     ENCADKYHFTREEQDAYATESVRRAQAAVKDGTFADEITTVSVPSRKGDVQVSTDETP
                     GKVDPAKIPNLKPAFRKENGTVTAASSSSISDGAAAVVLMSAENAKARGVKPLARIVA
                     HATHSQEPQWFTTAPVGAIDKVLKKAGWQVGDVDLFEVNEAFAVVAMAAMKELGIPHD
                     KLNVHGGACAMGHPIGCTGARIVVTLLHAMIHAGAKRGVASLCIGGGEATAIALERT"
     gene            1659..4427
                     /locus_tag="VFK00_11235"
     CDS             1659..4427
                     /locus_tag="VFK00_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7593563.1"
                     /translation="MGLLAGALAACGGGGNGGVRTLPEAPPPPPPPPPPSSTDQPPRD
                     AHLSLTNTYPAHDAGYDGSGITLGIVDSGIMRSNPTVAGRVKAELIYVDPSQNDTSVD
                     DVVGHGTWVSEIAAGTSFDQFPGGIAPGADLVSARIISDDSPSDHGQPPSVVTQQDAA
                     YFASVNADLITYGVDVMNNSWGGITWDTSSASVNQAFDDAYSPFVNQHGGLVVFAAGN
                     DSDANPSTIAALPSVAPDLEKGWLTVVAVDSNNPTELASYSNKCGVAMDYCLAAPGDV
                     IALDKDTLAGTTDPTYWIVSGTSLAAPEVSGAAALVWQAFPYFTNDLVRQTILGTADP
                     LGGSQPNPTFGYGELDVGRAVQGPAQFNWGDVSVSFDDITSTWSNDISGAGGLIKDGT
                     GTLILAGTDTYSGGTQVLGGSLQAANPLPGDVAVGGLGTLNAVPGVAGQLSNAGTVVV
                     QGGDTQVGGDYTQAGTGTLSISLGSKLDVSGSAQLDGTLNVLGEEPDYVTSDHQDVLE
                     AETVDGTFAQLTTSPGVFLSTSIEYTPTSVWLDTTSLSIEATAQTLGIAEPAAASAAV
                     RVQAGFDAIDTKIATGATLPPGVVKGAGAIQHTPTPAAARATLQSLSGQLHAASAAML
                     FDEIDANGLALSGRFDELLAGRSKAGAWYADAGWQGDLQRSGYAGATFRSDGGLVGAD
                     FRIGTHGLLGYAVGESRGYGQLDASWDRNRTSTDHLMLYGGAFSGPWYAAARVGGGWY
                     RQDMQRLLLLGGLAAPVGSGTSGHYLAGSLEGGYAFHAAAADVTPFVDVRYQRLDQGG
                     FAEQGGYGFGLMASEHQVGRTQAGVGLRAQSAWRLSNGMWVQLDGSAAWHHALHQYGD
                     AFDATFTAFDNWLPIDGVGLSRNESELRAGLSLWPTRSFALRLGLAREQGDRQRSGSV
                     MLQGGLRF"
     gene            4690..5199
                     /locus_tag="VFK00_11240"
     CDS             4690..5199
                     /locus_tag="VFK00_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HET7593564.1"
                     /translation="MQSVLVLHESRPRTRSLAATFLPNRLEWLMGLYGENHRRLARLF
                     APERLGVGEYVSSVDDGLDVRLSVQERHPYTLELGLSYAVIDASTGNPAPSAQLRMYL
                     DAHMTEALHCEPGRDLWQVLGPVAPARNVMLHRLRMNGFLNRWLDYLAGQGHSLGTLE
                     RRAPEDVAA"
     gene            5479..8382
                     /locus_tag="VFK00_11245"
     CDS             5479..8382
                     /locus_tag="VFK00_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HET7593565.1"
                     /translation="MEKKKLVVAVLAGLTVMTMTINLTACGGGGGGGNGMVKPTPPPP
                     PPPPPPPPPPPPPPPPPPPPPPPPPPPGSYDAHNNQLIPTSVLPAQQAGFLGTGVKVG
                     IMDSGIDPDDPTLNASDQSGRNKVAWFKSYDPDVSDPTAENDNFGHGTTGAQLIAGDP
                     YTDSSTDVVFKGGVAPGSSLYVAQICWGYPAGEVCHPSSGAYSDLIDQGVKVINESFG
                     DTQDVTTISSGSSKAQQTYNMLKPVKDAGILQVFGTGDTSTSQNPGVYAGLPYLFPSM
                     QPYIIAVTGVGIGQDGKPTGLYTENGGPTPCGVAADWCLSAPAQIYSVPVPPGFTTGY
                     SVGTSSATAIVTGVTAQVWQAFPWMTAPNMSDTVLTTATPIPGCAKEQCGWGMVNAEK
                     AVKGPGQFAFGEFNAKIPSGTTSTFGNDIGGDGSLTLSGPGTLKLTGTNTWTGGTSLD
                     AGTLLVEGSVASDVDVAAGATLAGTGTVAANVVNAGTVTSSSSAAGQGFTITGDLTDQ
                     SGSTTAVALGVPLTVGGTANLDGTMEVLAAPSDYTVHSTETLVNAGDISGTFADLSFA
                     SGVFYTGTLDYTSTEVTVTLSPVVDAATVLATPLASPQTLQSARNVQSAIDVSNHWIL
                     NNQTAGHEAWIQDAGRFLSAPTTANAVASLNSLSGEIYATSRALEVEQSLATDMAMAN
                     REHAQAVQGQQGIWVQSLGPTGSVAGNGYDPATYRGGGNLMGVGGPITGNFSAGLVAG
                     RSRVWSQMAGLGGRLDARQNVAGAYARWGTGVGYYLTGRVSYTTIRSQVQRELLLGET
                     LTGLSAQRNDHVTVTTLEGGRTWNFGAATLAPYVSVAGLHLQQDAFSEQGSAMGLAAP
                     SQADNVYFGTVGVRFGQEFAWAGGRSSLEGYAGYRHVFSGADLSMAASFSGVPEAVFA
                     AAGQDLSRNIGIVGAHWTNVVSDRWGWFLGADYRTGSGTADPLEAHVGVTVAF"
     gene            complement(8470..10662)
                     /locus_tag="VFK00_11250"
     CDS             complement(8470..10662)
                     /locus_tag="VFK00_11250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7593566.1"
                     /translation="MKSPLSLTMALALTGFVAGCGGTADASKDSESTAPQAQAATTET
                     TNPFFNESPLPLHFPQFDRITDSDYAPAFDRGMAEHLKEVQAIANNEAEPTFDNTIVA
                     LEKSGQILDRTATVFFSLSGVENNDTKKAIQAEYAPKLAAHRDAIMLDPKLFARIQAL
                     YDQRNDLGLDAQGVRLIERHHEDFVRAGAKLSEADKARVKEINGELAKLGSDFSQNVL
                     AEVNDSAVVVDSREELAGLTEAQIDAAAAAAKKKGLEGKYVIALVNTTIQPVIAQLDD
                     RALRRRIYEASVARGSRGGKYDTTAIVSKVMKLRAEKAKIMGYPNWAAYGLADQTAKT
                     PEAVNAMLGKLAPPAVANAKLEAADLQAMIDKTQKARGEPTFKLAAWDWAYYTEKVRA
                     DKYAFDESQLKPYFEMKNVLENGVFFAANQEYGISFKQRTDLPVYNPDVLVYDVFNAD
                     GSQLAIFLIDPYARPSKRGGAWMNSYVSQSRLTGDLPVVANHLNITKPASGPTLMTWD
                     EVTTMFHEFGHALHGMFSNVEYPYFSGTSVPRDFVEFPSQVNEMWADWPSVLAHYAKH
                     YETGEPMPSALLDKVLATSKFNQGFATTEYLGAAMLDQSWHQIGVDEVPTADGVMAFE
                     AAALKANGTDFAPVPPRYRTPYFSHIMSGYSAGYYAYIWSEVLDANTVEWFKQHGGLT
                     RENGDRFRETLLSQGGSKDAMELFTDFAGHEPRIEPLLERRGLDAAGG"
     gene            10865..11115
                     /locus_tag="VFK00_11255"
                     /pseudo
     CDS             10865..11115
                     /locus_tag="VFK00_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462828.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
     gene            complement(11210..11286)
                     /locus_tag="VFK00_11260"
     tRNA            complement(11210..11286)
                     /locus_tag="VFK00_11260"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11250..11252),aa:Asp,seq:gtc)
     gene            complement(11406..11481)
                     /locus_tag="VFK00_11265"
     tRNA            complement(11406..11481)
                     /locus_tag="VFK00_11265"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11445..11447),aa:Glu,seq:ttc)
BASE COUNT         1878 a         3817 c         3884 g         1966 t
ORIGIN      
        1 tcgttcggat tgctgcacgc gcccaacgcg agaaccatgg cgccggccaa ggcggacgcg
       61 agtacggtac gggtgaactt catgagcctg tctcctgatg gtgagccgtg gatagccacg
      121 gaagtggccg cgctattaaa cagcgtcccg cagaataacg ccagcattca gccattttac
      181 gaacacataa cgttcagtta ccggttgccg cactggtgct aaaaaccagg agcggattgc
      241 tcaggtcctt tcgagcgcga tggcggtcgc ctcgccgcct ccgatgcaca gcgaagcgac
      301 gccgcgcttc gcgcccgcat ggatcatcgc gtgcagcagc gtcaccacga tgcgcgcgcc
      361 ggtgcagccg attggatgcc ccatggcgca ggcgccgccg tgcacgttga gcttgtcgtg
      421 cgggataccg agctccttca tggccgccat ggcaaccacg gcgaacgcct cgttcacttc
      481 gaacaggtcg acgtcgccga cctgccaacc ggccttcttg agcaccttgt cgatcgcgcc
      541 gacgggggcc gtcgtgaacc actgcggttc ctgggagtgg gtggcgtgcg cgacgatgcg
      601 cgccagcggc ttcacgccgc gcgccttggc attttccgcg gacatcagca ccaccgcggc
      661 cgcgccatcg gagatactgg acgagcttgc ggcggtgacg gtgccgtttt ccttgcggaa
      721 ggcgggcttc aaattcggga ttttcgcggg gtcgaccttg cctggcgttt cgtcggtcga
      781 aacctgcaca tcgcccttgc gcgaggggac gctcaccgtg gtgatttcgt ccgcgaacgt
      841 cccgtccttc accgcggcct gggcgcggcg cacggactcg gttgcatagg cgtcctgttc
      901 ctcgcgggtg aagtggtatt tgtcggcgca gttctccgcg aacgtcccca tcgactggcc
      961 gtcgtaggga ttggtcaggc cgtcccacat catgtggtcg acgagcgcgc cttcgccgat
     1021 gcggatgccg gagcgtaccg ccgccatgtg cggggcgttg gtcatggatt ccatgccgcc
     1081 agcgaccacc acgtcggccg agccggcgac gatcaggtcg cggccgagca tgatcgcctt
     1141 cattcccgag ccgcacacct tgttgagggt ggtgcagccg gtggagggcg gaagcccggc
     1201 cgcgagcgcg gcctgccgcg ccggcgcctg gccgaggttg gccggcagca cgcagcccat
     1261 cagcacctcg ccgacatcgc ccggctcgac cccggattgt tgcagtgccg cgccgatggc
     1321 ggcggccccg agctgcgggg tgggaaagcc ggtgaactgg ccgaggaaag ccccgatggg
     1381 ggtgcgcttg gcggcggcga tgatgacgtc ggacatgaga tgtggctcca gaaaaggacg
     1441 tatcgcttga attaagctgg attgcggaga agcttcggct tgaatggaag cgctcttccg
     1501 taaattatgg ggtggatgtt gcgccgcggc aaacaggctg ggcaggcgca ccacgccggt
     1561 gcacaatggc tgcccgggac tcggagtgga cgagggggtt tctcatgcgg tgcgatgttt
     1621 cgcgggcctg ggttcgatat gcgggccgat ggatcacggt ggggctcctg gccggcgcgc
     1681 tcgccgcctg cggtggtggc ggcaacggcg gggtgcgcac cctcccggag gcacctccac
     1741 cgccgccgcc gccaccgcca ccctcgagca ccgaccagcc gccccgcgac gcgcacctct
     1801 cgctcaccaa tacctatccc gcgcatgacg cgggttacga tggcagcgga atcaccctcg
     1861 gcatcgtcga cagcggcatc atgcgcagca atccgacggt ggcggggcgg gtgaaggcgg
     1921 aattgatcta cgtcgatcct tcccagaacg acaccagcgt cgacgatgtc gtcggccacg
     1981 gcacctgggt gtccgaaatc gccgccggca cctcgttcga ccagttcccc gggggcatcg
     2041 cgccgggtgc ggacctcgtt tcggcgcgga tcatttccga cgactcgccc agcgaccacg
     2101 gccagccgcc cagcgtcgtg acgcagcagg acgcggcgta cttcgcctcg gtcaacgccg
     2161 acctgatcac ctacggcgtc gacgtgatga acaactcctg gggcggcatc acctgggaca
     2221 ccagctccgc gagcgtgaac caggcgttcg acgatgccta cagcccgttc gtgaaccagc
     2281 acggcggtct cgtggtgttc gccgcgggca acgattccga cgcgaatccc agcaccatcg
     2341 cggcgctgcc gagcgtcgcc cccgacctgg agaagggctg gctgacagtc gtggcggtcg
     2401 acagcaacaa tcccaccgag ctcgccagtt actcgaacaa gtgcggcgtc gcgatggact
     2461 attgcctcgc cgcgcccggg gacgtgatcg cgctcgacaa ggacaccctt gccggcacca
     2521 ccgatcccac gtactggatc gtctcgggca cgtcgctcgc ggcgccggag gtgtccggcg
     2581 cggccgcgct ggtgtggcag gcgttcccgt atttcaccaa cgacctggtg cgccagacca
     2641 tcctcggcac cgccgacccg ctcggggggt cgcaacccaa cccgacgttc ggctacggag
     2701 aactcgatgt cgggcgcgcg gtgcaggggc cggcgcagtt caactggggc gacgtgtcgg
     2761 tgtcgttcga cgacatcacc tcgacctgga gcaacgacat cagcggcgcg ggcgggctga
     2821 tcaaggacgg cacgggcacg ctgatcctgg ccggtaccga cacctacagc ggcggcaccc
     2881 aggtgctggg cggatcgctg caggccgcca acccactgcc gggcgacgtc gcggtcggcg
     2941 gactcggcac gctgaacgcg gtgccgggcg tcgcgggaca gctcagcaac gccggcacgg
     3001 tggtggtgca gggcggcgac acgcaggtgg gtggcgacta cacccaggcg ggcaccggca
     3061 cgttgtccat cagcttgggt tccaagctcg acgtgagcgg aagcgcacag ctcgacggca
     3121 cgttgaacgt gctgggcgag gaacccgact acgtcaccag cgaccaccag gatgtgctgg
     3181 aggccgaaac cgtcgatggc accttcgcgc aactcacgac ttcgccgggt gtgtttctct
     3241 cgacctcgat cgagtacacg ccgaccagcg tatggctgga cacgaccagc ctgagcatcg
     3301 aagcaacggc acagacgctg gggatcgccg aacccgccgc ggcatcggcc gcggtgcgcg
     3361 tgcaggccgg cttcgatgcg atcgacacca agatcgcgac cggtgcgacg ttgccgccgg
     3421 gtgtggtgaa gggcgcgggc gccatccagc acacgccgac gcctgccgcc gcgcgcgcga
     3481 ccctgcagag cctgtccggc cagttgcacg ccgcgagcgc ggcaatgctg ttcgacgaaa
     3541 tcgatgcgaa tggcctggcg ctgtcgggac gcttcgacga actgctggcc ggtcgcagca
     3601 aggccggcgc ctggtacgcc gacgcgggtt ggcagggcga cctgcagcgc tccgggtacg
     3661 ccggcgcgac cttccgcagc gatggcggcc tcgtcggtgc ggatttccgc atcggtaccc
     3721 atggtctgct gggttacgcg gtgggcgaga gccgcggcta cggccaactc gacgcatcgt
     3781 gggaccgcaa ccgcacctcg accgaccatc taatgctcta cggcggggcg ttcagcggac
     3841 cgtggtacgc cgcggcgcgc gtcggcggtg gctggtatcg ccaggacatg caacgcctgc
     3901 tgctgctcgg cggcctcgcg gcgccggtcg gcagcggtac gtccggccac tatctcgccg
     3961 gttcgctgga gggtggctac gcgttccatg cggcggcggc tgacgtgacg ccgttcgtgg
     4021 acgtgcgcta tcagcggctc gaccagggtg gattcgcgga gcagggcggc tacggtttcg
     4081 gtctcatggc cagcgagcat caggtcgggc gcacccaggc gggcgtgggc ctccgtgcgc
     4141 aaagtgcgtg gcggttgtcg aacggcatgt gggtgcagct cgacgggagc gcggcgtggc
     4201 accacgcgct gcaccagtac ggcgacgcgt tcgacgccac gttcacggcg ttcgacaact
     4261 ggctgccgat cgatggcgtg ggcttgtcgc gcaacgaatc cgagttgcgc gcggggctgt
     4321 cgctgtggcc gacccgctcg ttcgccttgc ggctcgggtt ggcgcgcgag cagggggacc
     4381 ggcaacgatc cggcagcgtg atgctgcagg gtgggttgcg gttctgagaa gggcggaccc
     4441 gccggtgttg acctgcgtcg cgtcgcgctc gaccgcgtcg cgttgcgggg tccttcactg
     4501 cgggtagggg cgttgcacgc cggtcgtggg cggcgcggga aaaatcgact gcggcgaacg
     4561 acgtccgcgc cacgtcgcga acaccccaaa agagttcggc gcccatgggg cgcccagacc
     4621 cgtacgttgc gcgttttccc gcgaaaatgc aaggggtgcg caaacgccat catgcgctaa
     4681 gatcggcgca tgcaatcggt acttgtcctg catgaatcca ggccgcgcac gcgctcgctc
     4741 gcggccacct tcctgcccaa ccgcctcgag tggttgatgg gcctctatgg cgagaaccac
     4801 cgccggctcg cgcggctgtt tgcacccgag cggctgggcg tcggcgaata cgtgtcttcg
     4861 gtggatgacg gactcgacgt gcgcctcagc gtgcaggaac gccatcccta caccctcgaa
     4921 ctcggcttga gctacgcggt gatcgatgcg agcactggca atccggcgcc gtcggcgcag
     4981 ttgcggatgt acctcgatgc gcacatgacc gaagctttgc attgcgaacc gggccgcgac
     5041 ctctggcagg tgctcggccc ggtcgcgccc gcacgcaacg taatgctgca tcgcctgcgc
     5101 atgaacggct ttctcaatcg ctggcttgat tatctggcag ggcagggcca ctcgctgggg
     5161 acgctggaac gcagagcgcc cgaagacgtc gccgcttagt tcccgaaacc gcttcccgtt
     5221 gcaccaggac acgtgccgtt cgaacgcgcg gccggcgtgc cgttggcgcg gtttgtttgg
     5281 gcccgcccga gccacagcgt ggggggattg atcgacacgc cgtctccaca tttcatacgc
     5341 taaaaaggcg cggtgcatgg aaggggcgaa gcgcggccct gtttgcggcc ggacgttcgg
     5401 caggccgatt acgcgcagtt tcgatcggat accatcgacc cgcgtcaggg tgtaaacgaa
     5461 ccatcaggga gatcggccat ggaaaagaag aagttggtgg ttgcggtgct cgccggcctt
     5521 acggtgatga cgatgaccat caatctcaca gcgtgtggtg ggggtggagg cggcggcaac
     5581 ggcatggtca aaccgactcc gccgcctcca ccgccgccgc ctccaccacc acccccgccg
     5641 cctccgccgc cgcctccacc gccacccccg ccgccgcccc cgccaccgcc ggggagctat
     5701 gacgcgcaca acaaccagtt gatcccgacc agcgtcttgc cggcgcaaca ggcaggcttc
     5761 ctgggcaccg gggtgaaggt cggcatcatg gattcgggca tcgatcccga cgatccgacc
     5821 ctgaacgcca gcgaccagtc gggccgcaac aaggtggcat ggttcaagag ctacgatccc
     5881 gacgtcagcg atccgacggc cgagaacgac aatttcggcc acggcaccac gggggcgcaa
     5941 ctgatcgcgg gcgatccgta caccgactcc tccaccgacg tggtgttcaa gggcggcgtc
     6001 gcgcccggtt ccagccttta tgtggcgcag atctgctggg gctatcccgc cggcgaggtt
     6061 tgccatccat cctcgggcgc gtattcggac ctcatcgacc agggcgtgaa ggtcatcaac
     6121 gaatccttcg gcgataccca ggacgtgacg accatttcct ccggcagttc gaaggcgcag
     6181 caaacctaca acatgctcaa gccggtcaag gacgccggca tcctgcaggt gttcggaacc
     6241 ggtgacacct cgacctcgca aaaccctggc gtctacgccg gcttgcctta cctgttcccg
     6301 agcatgcagc cctacatcat cgccgtgacc ggcgtcggga tcggccagga cggcaagccg
     6361 acggggctgt acacggagaa cggcggcccg accccctgcg gcgtcgcggc ggactggtgc
     6421 ctgtcggcgc cggcccagat ctactccgtg ccggtgcctc ccggcttcac caccggctac
     6481 agcgtgggca cctcgtctgc gaccgcgatc gtgaccggtg taaccgcgca ggtctggcag
     6541 gcattcccgt ggatgacggc cccgaacatg tccgacacgg tgttgaccac cgccacgccg
     6601 atccccggct gcgccaaaga gcaatgcggc tggggcatgg tcaacgccga gaaggccgtg
     6661 aaaggccccg ggcagtttgc gttcggcgaa ttcaacgcca aaattccttc cggtaccacc
     6721 tcgaccttcg gcaacgacat cggcggcgac gggagtctca cgctttccgg tcccggcacc
     6781 ctcaagctca cgggtaccaa cacctggacg ggcggcacca gcctcgatgc gggtacgctc
     6841 ctggtcgagg ggagcgtggc gtccgacgtg gacgtggctg ccggcgcgac gctcgcgggt
     6901 acgggtaccg tcgccgccaa cgtggtcaat gccggcacgg tgacgagttc cagcagcgcg
     6961 gccgggcagg gattcaccat caccggtgac cttaccgacc agtccggcag caccacggcg
     7021 gtggcgcttg gcgtaccgct cacggtcggc ggcacggcga acctcgacgg aaccatggag
     7081 gtgctggcgg cgccgtctga ctacaccgtc cattccaccg aaacgctggt caacgccggc
     7141 gacatctcgg gcactttcgc ggacctcagc tttgcatcgg gagtgttcta taccggtacg
     7201 ctcgactaca cctcgaccga agtcacggtg acgctgagcc ccgtcgtcga tgccgcgaca
     7261 gtcttggcga caccgctcgc gagcccgcag acgttgcagt cggccaggaa cgtgcagtcc
     7321 gcgatcgacg tgtccaatca ctggatcctg aacaaccaga ccgccggcca cgaagcctgg
     7381 atccaggatg cgggcaggtt cctctccgcg cccacgaccg cgaacgcggt cgccagcctg
     7441 aattcgctgt cgggcgagat ctacgccacc agccgcgcgc tcgaagtcga gcagtcactg
     7501 gcgaccgaca tggcgatggc gaaccgggaa cacgcccagg cggtgcaggg acagcagggc
     7561 atctgggtcc agtcgctcgg gcccaccggc tccgtcgccg gcaatggcta tgaccccgcc
     7621 acgtatcgtg gtggcggcaa cctgatgggg gtcggcggcc cgatcaccgg caacttctcg
     7681 gcgggtctcg tcgccgggcg cagtcgcgtc tggagccaga tggccggcct cggcggacgc
     7741 ctggacgctc gccagaacgt ggccggtgcc tacgcccgct ggggtaccgg tgtcggttac
     7801 tacctcaccg gacgcgtgag ttacaccacc atacgtagcc aggtccagcg cgagttgctg
     7861 ctcggcgaga cgctcaccgg cctgtccgcc cagcgcaacg atcacgtcac cgtgaccacg
     7921 ctcgaaggcg gcaggacctg gaacttcggg gctgcgacgc tggcaccgta cgtttcggtg
     7981 gcgggcctgc acctgcaaca ggacgcattc tcggagcagg gaagcgcaat gggcctggca
     8041 gccccgtcac aggccgacaa cgtctacttc ggaacggtcg gcgtccgctt cggtcaggaa
     8101 ttcgcctggg cgggggggcg ttcgtcgctg gagggttacg cgggttaccg ccacgtgttc
     8161 agcggtgcgg acctttccat ggctgcgagc ttctcgggcg tgcccgaagc ggtgttcgct
     8221 gccgcgggac aggacctgtc gcgcaacatc ggcatcgtgg gcgcgcactg gaccaacgtg
     8281 gtgtcggacc ggtggggctg gttcctcggc gccgactacc gaacgggcag cggaaccgcc
     8341 gacccgctcg aggcgcatgt cggcgtgacc gtcgcgttct gaggatcccg cgctcttgtg
     8401 cgagcgcgcg tggtcgatgc ggaaagcggt caggtccact tccgtcggaa gtggacctga
     8461 cccggttctt cagccgcccg cggcatcaag gccgcgccgc tccagcagcg gttcgatccg
     8521 cggctcgtgg ccggcgaagt cggtgaacag ctccatcgca tccttgctgc cgccctgcga
     8581 aagcagcgtc tcgcggaagc ggtcgccgtt ttcgcgggtc aggccgccgt gctgcttgaa
     8641 ccactcgacc gtgttcgcgt ccagcacttc ggaccagatg taggcgtagt agccggcgga
     8701 atagccgctc atgatgtggc tgaagtacgg cgtgcggtag cgcggcggca ccggcgcgaa
     8761 gtcggtgccg ttggccttca gcgccgccgc ctcgaaggcc atcacgccgt cggccgtggg
     8821 aacctcgtcg acgccgatct ggtgccagct ctggtcgagc atcgcggctc ccaggtactc
     8881 ggtggtggcg aagccctggt tgaacttcga cgtcgccagc accttgtcca gcagcgccga
     8941 gggcatcggc tcgcccgtct cgtaatgctt cgcgtagtgc gccagcacgc tcggccagtc
     9001 cgcccacatc tcgttcacct gcgaggggaa ctccacgaag tcgcgcggta cgctcgtgcc
     9061 cgagaagtac gggtattcca cgttcgagaa catgccgtgc agcgcatggc cgaactcgtg
     9121 gaacatcgtc gtcacttcgt cccaggtcat cagggtcggc ccgctggcgg gcttggtgat
     9181 gttgaggtgg ttggcgacga ccgggaggtc gcccgtcagc cgcgactggc tgacgtacga
     9241 attcatccac gccccgccgc gcttggacgg acgcgcgtac gggtcgatca ggaatatcgc
     9301 gagctggctg ccgtcggcgt tgaagacgtc gtacaccagc acgtccgggt tgtagaccgg
     9361 aagatccgta cgctgcttga agctgatgcc gtactcctgg ttggcggcga agaacacgcc
     9421 gttctccagc acgttcttca tttcgaagta cggcttcaac tggctctcgt cgaacgcgta
     9481 tttgtcggcc cgcacctttt cggtgtagta cgcccaatcc caggccgcaa gcttgaacgt
     9541 gggttcgccc ctggccttct gggtcttgtc gatcatcgcc tgtagatcgg cggcttcgag
     9601 cttggcgttg gccaccgccg gcggcgccag cttgccgagc atcgcgttta cggcttccgg
     9661 tgtcttcgcg gtctggtcgg caaggccata cgccgcccag ttcgggtagc ccatgatctt
     9721 cgccttctcg gctcgcagct tcatcacctt cgagacgatc gcggtggtgt cgtacttgcc
     9781 gccacggctg ccgcgcgcga ccgaagcctc gtagatccgc cggcgcaacg cgcggtcgtc
     9841 gagctgcgcg atcaccggct ggatcgtggt gttgaccagc gcgatcacgt acttgccctc
     9901 cagccccttc ttcttcgcgg cggccgcggc agcatcgatc tgcgcctccg tgagcccggc
     9961 cagctcctcg cggctgtcca ccaccacggc ggagtcgttc acctcggcca gcacgttctg
    10021 gctgaagtcg gagccgagct tggccagttc gccgttgatc tccttgaccc gcgccttgtc
    10081 ggcctcggaa agcttggcgc cagcgcgcac gaaatcttcg tggtggcgct cgatcaatcg
    10141 cacgccctgc gcgtcgaggc cgaggtcgtt gcgctggtcg tagagggcct gtatccgtgc
    10201 aaacagcttc gggtcgagca tgatcgcgtc gcggtgcgcg gcgagcttcg gcgcgtattc
    10261 ggcctggatg gccttcttgg tgtcgttgtt ctcgaccccc gaaaggctga agaaaacggt
    10321 tgcggtgcga tcgaggattt gcccggattt ttccagtgcg acgatcgtgt tgtcgaaagt
    10381 cggttcggcc tcgttgttgg cgatcgcctg gacttccttc aggtgttcgg ccatgccgcg
    10441 gtcgaacgcg ggcgcatagt cggaatccgt gatcctgtcg aattgcggga agtgcagcgg
    10501 caaggggctt tcgttgaaga acggattggt ggtttcggtc gtcgcggctt gggcctgcgg
    10561 cgcggtcgat tcggaatcct tgcttgcgtc ggcggtgccg ccgcaaccgg cgacgaagcc
    10621 cgtcagggcg agggccatgg tcagggacaa cggagatttc atggtcgatg gctcgtggca
    10681 aaagaatggc catcctaccc aagccacacg gcttttgccc atgacttaag gcatgcacca
    10741 accgggtcgc ggatacacga acaaggctgc gctgcggacg cgcgctcata ttcaagaaag
    10801 gtccactgcg gttttggtgc atggcgtggt tttcaggttg ccggcgcggg cgcggccgga
    10861 gcccgaagtc atcgcgaccc gcaacgaagc cgtggccggg cgtttcgtgc acgcgttgcg
    10921 cgccgcggtc gtgatggtca acacttcgtc gcgcttcagc gacgggggtg aactgggcct
    10981 tggtgcggaa atcgaatctc ctccacccgc ctgcacgcct acggtccgat gggcgccgag
    11041 gcgctgacga tcgagcgctt cgtgctgcgc ggccacggcg aggtgcgcca tcccgagagc
    11101 cgcagcacgg cctgagcctt tgagcggagc gcaatcaggg atgctcgcga cgtgtgcgcg
    11161 agcgccacga aaaagcccgc gcatggcggg ctccgaaaaa atccagaact ggcggagcgg
    11221 acgggactcg aacccgcgac ctccggcgtg acaggccagc attctaacca gctgaactac
    11281 cgctccgtcg tcctgaaaaa cctgagtttt ggtgccccgg aacaagcgtt gcgtccgcgc
    11341 gcggcgtgtt gcatgtcccg agccgcacat tgtacttccg tcactgcatg ccatggcaac
    11401 cgcgatggcg tccccacggg gattcgaacc ccggtcgcca ccgtgaaagg gtggtgtcct
    11461 aggcctctag acgatgggga cagcgtgggg atttggtgga gccagacggg atcgaaccga
    11521 cgacctctac aatgccattg tagcg
//
LOCUS       DASSNH010000302         7260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_926046, whole genome shotgun sequence.
ACCESSION   DASSNH010000302 DASSNH010000000
VERSION     DASSNH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926046"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFK00_11270"
     CDS             complement(<1..674)
                     /locus_tag="VFK00_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7593567.1"
                     /translation="MSSECSIRVDGLSKRYEVYARPSDRLRQMVLPHLRRSPRRQARR
                     WYRECWALRDVSFDVRRGETVGVVGRNGSGKSTLLQLICGTLTPTSGTVAVAGRVAAL
                     LDLGAGFNPEFTGRENVRLSGLLYGLSEEELNARHDAILDFAEIGDVIDQPVKTYSSG
                     MYVRLAFAVAINVSPDVLVVDEALSVGDEAFQRKCFARIDQIRAGGATVVFVSHAAST
                     VIELCNR"
     gene            complement(664..1491)
                     /locus_tag="VFK00_11275"
     CDS             complement(664..1491)
                     /locus_tag="VFK00_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7593568.1"
                     /translation="MTTSAYRLIDPFAPYLALTRHFALVVQMTKRNVAGRYRGSFAGL
                     FWSFLNPLLVLAIYTFVFGVIFKSRWNAQAADHFQYAMVLFAGLNINAFFAECANRAT
                     TLIVENPSFVKRVVFPLETLAWSTIGSALFHLLVSTSVLLVVSPFFTGGLPWTVVLFP
                     AVVLCFLPAVAGTVWLLASLGVYLRDLAQLIGIATMALMFLAPIVYPATLVPIRYRDL
                     LYLNPLTVIAEASQNVLVFGEPPAWSRLGIYLLVSALFAWLAFAWFEHTRKGFADVV"
     gene            complement(1673..3748)
                     /locus_tag="VFK00_11280"
     CDS             complement(1673..3748)
                     /locus_tag="VFK00_11280"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HET7593569.1"
                     /translation="MTTRPMPLARAWARLARGSPDRFVPAFVVAFAIWTMYVHLITAT
                     HSSFVALLQGLPLVAGVAVAAAIGWFLLPKPAAAAGTGVSDARASLAARENGSRVLPL
                     VALALASLWVGLLWAGMPYPVFFWLALLGTAGAWSWNLRGGSPGAQGDIPEPPRALQK
                     HAGWIVVLVAMAAVCVTLVASRPDPDDALHLSIPASLLRFPQQPVLLHDTLYRLPDAP
                     ILTPFYRLNSYDVLIGLLARIAGVDLHVIAYLVLPSLFAVFCILAWVYLLRRIVPERW
                     PSVLLILFLVVVALGEAHRAYGNFAFVRLFQGKAILATGMVPVIAGAALSFVRSGGLR
                     QWLLLFAAQAAAVGFAATALFVAPAAALLGLAGGWSADAKGSRRFVLGVLASAYVFAA
                     ARAVSSATRGGQGFVTSNPMPDMLQILDGTWGSWSTGVLLAALLAAWAFVRDPVRARY
                     LSAGAFFFLLAVLNPHTCRFVADRFVGVYTYWRLTWALPLPFLLAVVLDGIVWRALCV
                     RPKVLAAGACALLVGFAVTFGWRFGTLRADNGVTLGVPNIKVYPVEYRVARQLAAQVP
                     EQAVVLTPELVAFWLPTFVVHPQLLGVRLPYLTQSFAPPEAARRRVLMLYVAGQQRSP
                     DAAMLFAKALERYRLEAVVFARSAPWRGEMETILECQAFRPLASGPYDTWVRTGGPAS
                     LAASPARRR"
     gene            complement(3745..4572)
                     /locus_tag="VFK00_11285"
     CDS             complement(3745..4572)
                     /locus_tag="VFK00_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7593570.1"
                     /translation="MASGLLGDTPQRDYAGKLRLFNAYAARELREAIGGLSLAPDSRV
                     LDAGCGTGETVKWLHESLRGHGTVVGMDLATAHVRAARHVAPTSRSIVQADVLQAPFA
                     DGTFDLVWTVNTLNHLCNPVAGVRKLSRLLRAGGRIAVGQSSLVPDMYFAWDARLEAR
                     VNDAVRAYYRERYGRSEHEFTAVRSLVGWLRHAGLRDVRVRTRMIERSQPLAEADERY
                     LLECIFRGTWGERLRTYLSPGDFMELSRLCDPDDERYALRRPDFHFLQSLTIAVGTK"
     gene            complement(4602..5708)
                     /locus_tag="VFK00_11290"
     CDS             complement(4602..5708)
                     /locus_tag="VFK00_11290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7593571.1"
                     /translation="MKLALVVPGGVDRGGECRVIPALLALLERLSRTHEVHVFALRQE
                     SAAGCWRLAGATIHNLGDGWPYLRGIAAIRSEHRRAPFCVIHAIFSGPCGLIAVAAAK
                     LLRLPSTVHVTGGELVALRDIGYGGRLTWKGRLRERLALRGADAVTATSGPIVELLQA
                     LGARATRIPLGVDLRKWPPLTPHRRLGGRARLIHVASLNRVKDQATLLHALAMLAKTG
                     VDFEMDIVGTDTLQGEVATLIHALGLQRRIRLLGFKTQRELRPIVECADLLVMSSRHE
                     AGPFVLVEAAVAGVPTVGTAVGHIVEWAPSAALAVPVGNAERLADAIHTLLADEELRL
                     RVARAAQCRALSEDADYTARTFEALYLRLATGAP"
     gene            complement(5705..6835)
                     /locus_tag="VFK00_11295"
     CDS             complement(5705..6835)
                     /locus_tag="VFK00_11295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7593572.1"
                     /translation="MHVAQLNFLPAPPDLGIEELLERWPSLADIAELASVSGVRVSVL
                     QAAWHAERLTRNGIDYHFVDIAGARTPEDRGRCFAKVLAGMDADLLHAHGLCFVEDAA
                     AVFDRQAGALPLLFQDHADRLPRWWRRPRHRRRFAAAAGVAFTSLEQAQPFVAAGIFR
                     PSLRLFAIPESSSRFTPGGRARARSETGLYGDPCVLWVGRLTRGKDPLTVLDGISRVA
                     ERLPALRLWCAFAAAPMLAEVRARIDGDPRLAGRVHLLGKVPHARIQSLLRSADIYVS
                     GSRAEGSGYAALEAMACGTTPVLTDIPAFRALTGNGRVGRLYPCGDPGRLAGALFAAA
                     TEAESRGCVRAHFDAELSFEAVGRLWADAYAQVFDGWRRRAA"
     gene            complement(6845..>7260)
                     /locus_tag="VFK00_11300"
     CDS             complement(6845..>7260)
                     /locus_tag="VFK00_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET7593573.1"
                     /translation="NPACEIIVYVYTPLPERSMHAKDRGKGRAPALLDARGEPVVFPA
                     TAGEWTQPQWVAYACHADAPWLTDRLRRRIQDFVTVLRCRFPTVQDLRSPSWAKRGLS
                     AMAAWRYRFGRYDRPWELDLANRMVRLRMPQVSGL"
BASE COUNT         1344 a         2527 c         2429 g          960 t
ORIGIN      
        1 cggttgcaca actcgatcac cgtactcgcg gcgtgcgaga cgaacaccac cgtggcgccg
       61 cccgcccgga tctggtcgat acgggcgaag cacttgcgct ggaacgcctc gtccccgacc
      121 gaaagcgcct catccaccac caggacatcg ggcgacacgt tgatcgcaac cgcaaacgcc
      181 agccgtacat acatgccact ggaataggtc ttgaccggct ggtcgatcac gtcgccgatc
      241 tcggcaaaat cgaggatggc gtcgtggcgg gcattcaact cctcctccga cagtccgtac
      301 aggaggccgc tgaggcggac gttttccctg ccggtgaact cgggattgaa gccggcgccc
      361 aagtccagca gcgccgcgac gcggcccgcc acggcgaccg tgccggaggt gggcgtcagc
      421 gttccgcaga tcagctgcag cagcgtcgac ttcccggaac cgttgcggcc gacgacgccg
      481 accgtctccc ctcggcgcac gtcgaaactg acgtcgcgca aggcccagca ttccctgtac
      541 cacctgcgcg cctgtcgccg aggcgaccgt cgcaggtgcg gcaggaccat ttgcctgagc
      601 cggtcggaag gtcgagcata gacctcgtag cgcttcgaca agccgtccac gcggatgctg
      661 cattcagacg acatcggcga agcccttgcg cgtgtgctcg aaccaggcga atgccagcca
      721 cgcgaagagc gccgacacca gcaggtagat gccgagccgg ctccacgccg ggggttcgcc
      781 gaacaccagg acgttctggg atgcctccgc gatgaccgtc aatggattga ggtacaggag
      841 gtcccggtac cggatcggaa ccagggtcgc cggatagacg atcggcgcca ggaacatcag
      901 cgccatggtg gcgatgccga tgagctgagc gaggtcgcgc aggtaaacgc ccagcgacgc
      961 cagcaaccag accgtaccgg cgacagccgg caggaagcac agcacgacgg ccgggaacag
     1021 gaccaccgtc cagggaagcc cgcccgtgaa gaacggcgac accaccagca acacgctcgt
     1081 ggacacgagc agatggaaca gcgccgagcc gatcgtgctc caggcgaggg tctcgagcgg
     1141 gaacaccacg cgtttgacga agctggggtt ctcgacgatg agcgtggtgg cgcgattggc
     1201 gcattccgca aagaacgcat tgatgttcag tccggcgaac aacaccatgg cgtactggaa
     1261 atggtcggcc gcctgcgcgt tccagcgcga cttgaagatc accccgaaca cgaaggtgta
     1321 gatcgccagc accagcaacg gattgaggaa tgaccagaac aatccggcga acgagccgcg
     1381 atagcggccg gccacgttgc gcttcgtcat ctgcacgacc agcgcgaaat gccgcgtcaa
     1441 cgccaggtag ggcgcaaagg gatcgatcag tctgtaggcg gatgtcgtca tggcaggtat
     1501 cgcttccatg ccacacggct tcggtgacga cctggaatcc agggcgcgcg gtggattggc
     1561 tcgtagacgt gagtcccgcg ccgccgtgct ttggttgcag tggtggcggt tgcgtcttgc
     1621 gcgacgggca ccgccgatcg ggagcgaagg tggcgggatc ggggaggccg cttcagcgtc
     1681 ggcgggccgg actcgccgcc aacgaagcgg ggccgccggt cctcacccag gtgtcgtagg
     1741 gtccggacgc gagtggccgg aacgcctggc attccaggat ggtctccatc tcccctcgcc
     1801 aaggggcgga gcgcgcgaac acgaccgcct ccaatcggta gcgttcgagc gccttcgcga
     1861 acagcattgc cgcatcgggc gatcgttgct gccctgcgac ataaagcatc aggacacgcc
     1921 gccttgcagc ctcgggcggg gcgaagctct gcgtgaggta gggaaggcgc acgcccagga
     1981 gctgcgggtg cacgacgaac gtcggcagcc agaaggcgac cagttccggc gtgagcacga
     2041 cagcctgctc cggtacctgt gcggccaatt ggcgggccac ccggtactcg acgggataca
     2101 ccttgatatt cggtacgccg agtgtcacgc cgttgtcggc ccgcaaggtt ccgaatcgcc
     2161 agccgaatgt cacggcgaac cctaccaaca gcgcgcaagc tccggcagcc agcactttcg
     2221 gccgcacgca cagggcgcgc cagacgatgc catccagcac cactgcaagc aggaatggca
     2281 acggcagggc ccaagtcaat cgccagtagg tgtacacgcc cacgaaacgg tccgccacga
     2341 aacggcaggt atggggattg agcaccgcaa gcaggaagaa aaaggcgccg gccgacagat
     2401 aacgcgcgcg gacggggtcg cgcacgaatg cccacgcggc aagcagcgct gccagcagca
     2461 ccccggtgct ccacgagccc caagtaccgt cgaggatctg gagcatgtcc ggcatcggat
     2521 tggaggtgac aaaaccctgc ccaccccggg tggcagacga cactgcccgc gcggcggcaa
     2581 agacatacgc cgatgcgagc acgcccagca cgaatcgccg gctgcccttc gcgtctgccg
     2641 accagccccc ggccaggccc agcagggccg cggcaggcgc cacgaacaag gcggtcgcgg
     2701 cgaatcccac tgccgccgcc tgcgcggcaa acagcaacaa ccactgccgc aatccgccgc
     2761 tgcgaacgaa cgacaacgca gcgccggcga tcacggggac catgcccgtc gccaggatcg
     2821 ccttgccctg gaacagcctg acgaatgcga aattgccgta ggcgcggtgg gcttcaccca
     2881 gtgccacgac gaccaggaac aggatcagca gcaccgacgg ccatcgttcc ggcacgattc
     2941 gccgcaacag gtagacccac gccagtatgc agaacacggc gaacagcgaa ggcaggacca
     3001 ggtacgcaat cacgtggagg tcgaccccgg cgatgcgggc cagcaggccg atcaggacgt
     3061 cgtagctgtt cagccggtag aacggcgtca ggatcggagc atcgggcaac cggtagaggg
     3121 tgtcgtgcaa caagaccggt tgctgcggga accgcaacag gcttgcggga atgctgaggt
     3181 gcagggcgtc gtcggggtcc ggccggctcg cgaccagggt gacgcacacg gcggccatgg
     3241 ccaccaggac gacgatccac ccggcatgct tctgcaaggc gcgcgggggt tcggggatgt
     3301 cgccctgcgc accgggcgat ccgccccgga gattccatga ccacgccccg gcggtgccca
     3361 gcagggcgag ccaaaagaac acggggtaag gcatgccggc ccacagcaat cccacccata
     3421 gcgacgcgag tgccaaggcc acgagcggca ggacacggct gccgttttcc cgggcggcca
     3481 gcgatgcacg cgcgtcggaa accccggtac cagcggccgc agccggtttc ggcaggagga
     3541 accatccgat cgccgcggcc accgccaccc ccgccaccag cggcaacccc tggagcaagg
     3601 ccacgaaact ggaatgggtc gcggtgatca ggtggacgta catcgtccag atcgcgaaag
     3661 ccaccacgaa cgccggtacg aatcgatccg gcgaaccgcg agcgagccgt gcccaggcgc
     3721 gcgcgagcgg catggggcgg gtcgtcattt cgtccccacc gcgatcgtca ggctctgcag
     3781 gaaatggaag tccgggcggc ggagcgcata acgttcgtcg tccggatcgc acagtcgcga
     3841 caattccatg aagtcgccgg ggctcaggta cgtccgcagg cgctcgcccc acgtgccacg
     3901 aaagatgcat tcgagcaggt aacgctcgtc cgcttccgcg agcggttggc tgcgctcgat
     3961 catccgcgtc cgcacgcgca cgtcgcgcaa acccgcgtgc cggagccagc cgacgagcga
     4021 gcggaccgcg gtgaattcgt gttcgctgcg accgtagcgc tcccggtaat acgcgcgcac
     4081 cgcgtcgttc acgcgggctt ccaggcgggc atcccacgcg aagtacatgt ccggcaccag
     4141 cgagctttgt cccaccgcga tccgtccgcc ggcgcgcagc aaccgcgaga gcttccgtac
     4201 accggcgacc ggattgcaca gatgattgag cgtgttcacg gtccacacca gatcgaacgt
     4261 gccgtccgcg aacggcgcct gcagcacgtc ggcctgcacg atcgacctac tggtcggcgc
     4321 gacgtggcgg gcagcccgga cgtgcgcggt ggcgagatcc atccccacca ccgttccgtg
     4381 accccgcagc gattcgtgca accatttgac cgtttcgccg gtgccgcaac cggcgtcgag
     4441 cacccgcgaa tccggcgcca gcgaaagccc gccgatcgcc tcgcgcaact cgcgcgccgc
     4501 gtaggcgttg aacaatcgga gcttccctgc atagtcgcgc tgcggcgtat cgccgagcag
     4561 gccggacgcc atcgttcgcg gttgcagggc gggccgcatg ctcagggcgc gcccgtggcc
     4621 aggcgcaggt acagcgcctc gaacgttcgc gcggtgtaat cggcgtcttc cgaaagcgcc
     4681 ctgcactgcg ccgcccgtgc cacccgtagc cggagttcct cgtcggcgag cagtgtatgg
     4741 atcgcgtccg ccaatcgttc cgcattgccc acgggcacgg ccagcgccgc cgacggcgcc
     4801 cattcgacga tgtggcccac ggccgtgccg accgtcggca cgccggcgac cgccgcctcg
     4861 accagcacga aaggcccggc ttcgtggcgc gacgacatca ccagcaggtc ggcgcattcc
     4921 acgatcgggc gcagttcgcg ctgcgtcttg aagccgagaa gccgtatgcg ccgctgtagc
     4981 ccgagcgcgt ggatgagcgt cgcgacctcg ccctgcaatg tgtcggtccc gacgatgtcc
     5041 atttcgaaat cgacgccggt tttcgccagc atcgcgagcg cgtgcagcag ggtcgcctgg
     5101 tccttgacgc ggttgagact ggcgacgtgg atcaaccgtg cgcgcccgcc gaggcgccga
     5161 tgcggcgtca acggcggcca tttcctgaga tcgaccccga gcggaatgcg cgtggcccgc
     5221 gcgccgagcg cctgcagcag ctcgacgatc ggcccgctgg tggccgtgac ggcgtcggcg
     5281 ccgcgcagcg cgagtcgttc gcgcaacctg cccttccagg tcaggcggcc gccgtacccg
     5341 atgtcgcgga gcgccacgag ctcgccgccc gtgacgtgga ccgtgctcgg caaccgcagc
     5401 agcttcgccg cagccacggc gatcaggccg cacggcccgg aaaagatcgc atggatcacg
     5461 cagaaaggtg cccggcgatg ttcggagcgg atcgcggcga tgccgcgcag gtacggccag
     5521 ccgtcgccga ggttgtgaat ggttgcgccg gcgagccgcc agcagccggc cgccgattcc
     5581 tgccgcaacg cgaacacgtg cacttcgtgg gtccgcgaca ggcgctccag cagcgccagc
     5641 agcgccggta tcacgcggca ctcgccgccg cgatccaccc cgcccggcac caccagtgcg
     5701 agcttcatgc cgcccgcctc cgccagccgt cgaatacctg cgcgtaggca tccgcccaca
     5761 accgccccac cgcttcgaac gaaagctcgg cgtcgaagtg cgcacgcacg cagccccgcg
     5821 actcggcttc ggtcgccgcg gcaaagagtg cgcccgccag ccgtccgggg tcaccgcacg
     5881 ggtacaagcg tccgacccgg ccgttgccgg tcaacgcgcg gaacgccggg atgtccgtca
     5941 acaccggcgt cgtgccacag gccatcgcct ccagcgcggc gtaaccgctg ccttcggcgc
     6001 ggctgcccga cacgtagatg tcggccgatc gcagcaatga ctggatcctc gcgtgcggca
     6061 ccttgccgag caggtgcacg cgtccggcga ggcgcggatc gccgtcgatg cgtgcccgca
     6121 cctcggccag catcggcgcc gccgcgaagg cgcaccaaag ccgcagggcg ggcagacgct
     6181 ccgcgacgcg cgagatgccg tccagcaccg tcaggggatc cttgccgcgg gtcagccgtc
     6241 ccacccacag cacgcatggg tcgccgtaca aacctgtctc gctgcgcgcg cgcgcacggc
     6301 cgcccggcgt gaaccggctg ctggattcgg gaatcgcgaa aagccgcagc gagggacgga
     6361 agatgccggc cgcgacgaac ggttgcgcct gctcgaggga ggtgaaggcg accccggcgg
     6421 ccgcagcgaa gcgccggcgg tggcgcggtc gccgccacca gcgcggcagg cgatcggcgt
     6481 ggtcctggaa aagcagcggc aatgccccgg cctgacggtc gaataccgcg gccgcgtctt
     6541 cgacgaagca caggccgtgc gcgtgcagca ggtcggcgtc catcccggcc agcaccttgg
     6601 cgaagcagcg cccgcggtcc tccggcgttc gcgccccagc gatgtcgacg aagtggtaat
     6661 cgatgccgtt gcgggtcagc cgttccgcgt gccaagccgc ctgcagcacc gacacccgca
     6721 cacccgaaac cgacgccagt tcagcgatgt cggcaagcga gggccaacgc tcgagcaatt
     6781 cctcgatgcc cagatctggg ggcgcgggca ggaagttgag ctgcgccacg tgcatgggtg
     6841 cggcctacaa gccggaaacc tgcggcatgc gcaggcgcac catccggttc gccaggtcca
     6901 gttcccatgg ccggtcgtag cggccgaagc ggtagcgcca cgcagccatc gcgctgagcc
     6961 cgcgcttcgc ccaggacggt gaacgcaggt cctggacggt ggggaaacgg cagcgcagca
     7021 ccgtcacgaa atcctggatg cggcggcgca gccggtcggt cagccagggc gcatcggcgt
     7081 ggcaggcgta ggcgacccac tgcggctgcg tccactctcc cgcggtcgcg ggaaacacga
     7141 ccggttcgcc gcgggcgtcg agcaacgccg gcgcccgccc cttgccgcga tccttcgcat
     7201 gcatgctgcg ttcgggaagc ggcgtgtaga cgtagacgat gatttcgcag gccggattga
//
LOCUS       DASSNH010000303        10586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_7904, whole genome shotgun sequence.
ACCESSION   DASSNH010000303 DASSNH010000000
VERSION     DASSNH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7904"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..997)
                     /locus_tag="VFK00_11305"
     CDS             complement(140..997)
                     /locus_tag="VFK00_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7593574.1"
                     /translation="MDDWRAGTVLVTGASSQIGCFLLPELVRAKVRVLALSRTRQPAL
                     AGVDWLQGALPDPPRLAAQANSICSCGPLDALAAWIAAGVAPGLERVVATSSMSAESK
                     QASPVPAERAVSRRLRDGEAALARACGQRGIEWTILRPTLIYGAGRDRSLTPLARRAM
                     RWRMFGLPAGSGLRQPVHAADVAIAVLRALDGAAAGRVVPVGGGERLSALAMFERVRA
                     SLPVRTCPVRIPASAVRLGARLVPALRGPLTRLEQDLVADNGDLERLLAVHPRGFAPD
                     RDCWSHVRG"
     gene            complement(1185..2864)
                     /locus_tag="VFK00_11310"
     CDS             complement(1185..2864)
                     /locus_tag="VFK00_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593575.1"
                     /translation="MNGRQDSNGNPRRTEPVLGNLDQVDAGEDRRAARPAQRPSPKRP
                     PSAAGPPREPERRSRRGWWIALVIVLVLAGAGAWAFTNQATLRGLLPQTQLNTLLTRA
                     DDALAAGKLTGGPDSARELYEAARVLDPDNERALAGLQKVGNEELERALTALKRRDYA
                     SARSDLEQARSLLGGGTGVEAVDNELAKAVLHDANINQLISQARAALANGRIEGHSGA
                     AALFGKVLAGDPQNSVARHGMEQVGDVLATRVQTQLGNGDRAEARKTLSTLAELLPRY
                     SQLPTLRAAVAAADRAADTQRDQYLAHGEADLRAGKITGDGSDNAEAQFKAALAVDPG
                     NAKAQEGLGNVAQALVVQANAAIDAGHPREADALLGHAAALAPKSTDLVAARSRLDAG
                     PPEVSEPASSGAGATTAVTPADSAKVARLVARAGAAAQKGDIMLPPGDSAYDLYRAAL
                     GIDGDNAQAQAGLRALPKITRDQFNRALGSGDLDHAHDMLATLEQLDPGEPAADAMRH
                     RLGSAWLDRAGHDARLGRPAAARTALEEARQLVPEDPRIGAVGAMINGDHP"
     gene            complement(3034..5124)
                     /locus_tag="VFK00_11315"
     CDS             complement(3034..5124)
                     /locus_tag="VFK00_11315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7593576.1"
                     /translation="MNSPPQAPSECAPAAPVDGLPAGERALWAQTDALLCDLGCDDDT
                     RAAAAWYAVACARPEAWRDAVPQVTAAVCRLVEGQQQAERVWALHAARDGKRGNEGLR
                     RLLLAIIRDLRVVYLLLARQLAEMRAAASLPEAQRRELAVRTRDIHGPLANRLGIGQW
                     KWELEDLAFRYLQPELYRRIAKLLDERRADREAWIGRCVAALRDALAAHGITATVNGR
                     AKHIYSIWRKMQRKGIGFGELYDIRALRVLTGSVADCYAVLGVVHALWPIIPQEFDDY
                     IARPKGNHYRSLHTAVIGPDGKTLEVQIRTDEMHRGAELGVAAHWRYKEGGSGDDAYE
                     ARVAWMRKLLEARAEGEDDSALLAELRSELAEERVYLLTPKGEVIDLQAGATVLDFAY
                     HVHSMVGHRCRGAKVNGRIVPLTHQPASGDRIEILTAREPAPSRDWLSLQLGYLNTAS
                     ARGKVRAWFRREDLAANTAAGRQLLEREARRLAVPDEVIERLPAKLGRKGRDDLLVAL
                     ALGEIATAQLTRAMLEAQAPPPAAPAPPPRPPRAPGQPGSLTVEGIGNLLTSLARCCR
                     PLPGDEVTGYVTRGRGVTVHRADCVALARLRARNPERVIEVRWQSAPDRAYEVSIAVV
                     GYDRRDLQRDVTNVIANSGARIVASETRSDRDVGEIAMHFALRVRDYGELSALLAKLG
                     AVANVTDVRRLAGG"
     gene            5246..5710
                     /locus_tag="VFK00_11320"
     CDS             5246..5710
                     /locus_tag="VFK00_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593577.1"
                     /translation="MRIRLMLPATLAVLIAAMPVMAQQFSSLEERMSAADFKAAGLDT
                     LTPQQLQFLDDWLRTHEQTRMVSPSGSPVFYPESEPRDKFDTHLVGHFSGWGGHSVFT
                     LDNGQVWKQAESGAYSCPDVDNPEVTIKPMILGSWLMYVQGCNQSVRVERVK"
     gene            5753..6217
                     /locus_tag="VFK00_11325"
     CDS             5753..6217
                     /locus_tag="VFK00_11325"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HET7593578.1"
                     /translation="MMNRAARAQARENRDVLEVWLPLLGLMALGAAWYHVLRLRERAI
                     LHARQLCDRHGLQLLDDSVGLHRLHARWRHGALQVTREYRFDTSLGGDDRRTASITLL
                     GDRIVATRLPSREPPAGAAPVATSPFVPATLARGGELPDNVVPLDRRRRTLH"
     gene            complement(6438..7346)
                     /locus_tag="VFK00_11330"
     CDS             complement(6438..7346)
                     /locus_tag="VFK00_11330"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET7593579.1"
                     /translation="MADTVTPERLLAGVPRIESPFFDEIFARLDPDAETLRVARDLHD
                     RGFAVIDFPDEDFERHAEAIKHDLGERFDLGAWRKKGGSMRIQDAWTFHPDVRALAVN
                     KHILELLSLLYGRSAWPFQTLNFPVGTQQPRHTDAVHFHSMPERFMCGVWVALEDVEL
                     DSGPLIYYPGSHKLPIYDNDQLGVEAADLNPHPGQRLYQPLWDALVAKHGLQPQRFAA
                     RKGQALVWTANLLHGGARHDDRSRTRWSQVTHYYFDDCVYYTPMHSNPAAGRIDRRWP
                     RNVLDGRPVRSAGVLRRILHRLRYGG"
     gene            complement(7444..7965)
                     /locus_tag="VFK00_11335"
     CDS             complement(7444..7965)
                     /locus_tag="VFK00_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HET7593580.1"
                     /translation="MTSNRPYLLRAVHEWICDNGLTPYVVVDASQPGIQVPPQSVSDG
                     RVVLNLAPRAVARLEIGNDAITFMARFGGVSHAVTLPIAAVQAIYARENGQGMLLAED
                     ATIATAPIGPAADPQPPESEPPKPTRPASPLQAVPASSRDKPAPGDSASSGQGDGPDK
                     PRPRGKPNLHVIK"
     gene            complement(7980..8606)
                     /locus_tag="VFK00_11340"
     CDS             complement(7980..8606)
                     /locus_tag="VFK00_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7593581.1"
                     /translation="MIQTAHTRTVLTLFTAPDDVQSHRVRLVLAAKGVVYDRVEVPAG
                     KPHDELLSINPYGDTPTLQDRDLVLYEPDVVCEYIDERYPHPPLMPVDPLSRARLRLA
                     CVRIERDWVPEVASIKAGGRTAEPARKRLREHLMTALPLFKAAKFFLNPEISLADCLV
                     APVVWRLPSLGVSLPREAKPIIDYGERLFRSQGFARSLTSDERLLGEL"
     gene            complement(8695..9420)
                     /locus_tag="VFK00_11345"
     CDS             complement(8695..9420)
                     /locus_tag="VFK00_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HET7593582.1"
                     /translation="MTAVLLALLVALAAPAAFAEEGGFPNANVNVHDQGALQRGARLF
                     MNYCAGCHSLKYMRYSQMAEGLGLTEKQVMDNLNFTGAKFGEPIVSAMPEADAEKWFG
                     KAPPDLSLEVSAKGADWVAAYMKSFYLDPSTTSGWNNTVFPNVAMPFPLWQLQGEQVA
                     VMNPPANGQPGTVKELKLAQPGSMTPAQYDRAVRDLTAFLAYVSSPDELVRSRMGAWV
                     VIYLVLFTLFALVLKKEYWKDVH"
     gene            complement(9449..>10586)
                     /locus_tag="VFK00_11350"
     CDS             complement(9449..>10586)
                     /locus_tag="VFK00_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7593583.1"
                     /translation="VLVNQIVTGIFLTMFYTPTAAGAFDSVQYIMRDVNWGWLVRYMH
                     TTGASMFFIVVYLHMFRGLMYGSFKKPRELVWLVGMLIFLVLMAEAFMGYVLPWGNMS
                     FWGAKVIITLFSAVPWFGHTLVTWIMGTFVPGDPTLNRFFALHVVALPLVLLLLVVLH
                     LGALHEVGSNNPDGVEIKKHKNPKGIPLDGIPFHPYYTVKDLVGVGVFLVICAVIIFF
                     APSVGGFFLEHDNSIPANDLVTPATIKPVWYFTPFYAILRMIPSKGLGVLALAASIVV
                     LFLVPWLDRGGVKSIRYRGLAYKIALAIFAIVFVLLGCIGMDMTAGWIVAIFGPDADI
                     TTIENLFGRILVILYFAFFVFLWVYTFFGLERTRSVPERVTTHA"
BASE COUNT         1705 a         3664 c         3533 g         1684 t
ORIGIN      
        1 gctcgcttgc aaggctttcg tgatggtgaa aagagatctg acgcggatga acgcggatga
       61 ccgtcgatca aaaatctttt tatccgcatc gatccgcgtt gatccgagtc ggaaatcgct
      121 tgtcgccaac ggaccgccgt cacccgcgca catggctcca gcagtcgcgg tccggggcaa
      181 aaccacgcgg atgcacggcg agcaggcgtt cgagatcacc gttgtcggcg accaggtcct
      241 gctccaggcg ggtcagcggc ccgcgcagcg ccggcaccag gcgcgcaccc aaccgcacgg
      301 ccgacgccgg aattcgcacc gggcaagtac gcaccggcag gctcgcgcgc acccgttcga
      361 acatcgccag tgcggagagg cgttccccgc caccgaccgg gactacccgt cccgcggcag
      421 cgccatcgag tgcgcgcagg accgcgatcg cgacatcggc ggcatggacc ggttggcgca
      481 gcccggatcc cgcgggcaat ccgaacatcc gccaccgcat cgcacgtcgc gccagcggcg
      541 tcaggcttcg gtcgcgcccg gcaccgtaga tcagcgtcgg ccggaggatc gtccactcga
      601 tcccccgttg cccgcacgcc cgcgcgagcg ccgcttcgcc gtcacgcagg cggcgcgaca
      661 ccgcgcgctc cgccggcacg ggtgatgcct gcttgctttc cgcgctcatc gagctggttg
      721 ccacgacccg ctcgagaccg ggcgccacgc ccgccgcaat ccatgctgcc aacgcgtcca
      781 aggggccgca cgaacagatc gaattggcct gagcggccag gcgcggcgga tccggcaacg
      841 cgccctgcag ccagtccacg ccggcgagcg ccggttgccg cgtacgcgac agcgcgagga
      901 cacgcacctt cgcgcgcacg agttccggaa gcaggaaaca gccgatctgg ctgctggcac
      961 ccgtgaccaa taccgtcccc gcgcgccaat catccacgtc gcgttccccc gacgactcca
     1021 cggagccaat gccgtggaag tgtaaacacg tggcacctgc gccggtcgaa agcgcggttc
     1081 ccgacgcgga tcaacgagat cgaccgatga agaacatcga tccgcgtcaa aaaacatatt
     1141 gtcaccatcg cccggatcgg caaaccatcg ccgtgtgacg gcgatcaggg atgatcgcca
     1201 ttgatcatgg cgccgaccgc gccgatccgg ggatcctccg gcaccagttg ccgcgcctct
     1261 tccagcgccg tgcgcgccgc cgccggacgc cccaggcgcg cgtcgtggcc ggcacgatcg
     1321 agccatgcgc tgccgagccg atgccgcatc gcatccgccg cgggttcgcc cggatcgagt
     1381 tgttccagcg tcgccagcat gtcatgggca tgatcgagat cgccgctccc gagcgcccgg
     1441 ttgaactggt cgcgggtgat cttcggcaac gcccgcaggc ctgcctgcgc ctgcgcattg
     1501 tcgccatcga tgccgagcgc ggcacgatac aggtcgtagg cgctgtcgcc gggcggcagc
     1561 atgatgtcgc ccttttgcgc cgccgccccg gcgcgcgcca ccagccgcgc gaccttggcc
     1621 gagtcggccg gcgtcactgc cgttgtcgcg ccggcgccag aagaagccgg ctccgatact
     1681 tccggcggac ccgcgtcgag gcgcgagcgg gcggcgacca ggtccgtcga tttcggcgcc
     1741 aaggcggcgg cgtggccgag cagcgcatcg gcttcccgcg ggtgtccggc gtcgatggcg
     1801 gcgttcgctt gcacgaccag ggcctgcgcc acgttgccca ggccctcctg ggccttcgcg
     1861 ttgccgggat cgaccgccag cgcggccttg aattgcgcct ccgcgttgtc gctgccgtca
     1921 ccggtgatct tgcccgcgcg caggtcggcc tcgccgtgcg cgaggtactg gtcgcgctgc
     1981 gtgtccgcgg cgcgatcggc cgcggcaact gccgcgcgca gggtcggcaa ctgcgaatag
     2041 cggggcagca gctcggcgag ggtcgacaat gtcttccggg cctcggcgcg atcgccgttg
     2101 ccgagctggg tctggacgcg cgtcgcgagc acatccccca cctgttccat gccgtggcgc
     2161 gccacgctgt tttgcggatc gcccgccagc accttgccga acagcgccgc ggcaccgctg
     2221 tggccttcga tgcggccgtt cgcgagcgcg gcgcgcgcct gcgaaatgag ctggttgatg
     2281 ttggcatcgt gcagcacggc cttggccaat tcgttgtcca cggcttccac gccggtgcca
     2341 ccgcccagca ggctgcgcgc ctgctcgaga tcactgcgcg cgctggcgta gtcgcgacgt
     2401 ttcaaggccg tcagcgccct ttcgagctcc tcgttgccga ccttctgcaa gcccgccagt
     2461 gcacgctcgt tgtcggggtc gagcacgcgc gcggcttcgt acagttcgcg tgcgctgtcg
     2521 gggccgccgg tcaacttgcc ggccgccagc gcgtcgtcgg cacgcgtcaa gagggtgttc
     2581 aactgggttt gcggcagcag cccgcgtaac gtcgcctggt tcgtgaaggc ccacgcgccg
     2641 gcgccggcca gtaccaggac gatgacgagc gcgatccacc agccgcgacg gctgcggcgc
     2701 tccggttcac gcggcggccc ggctgcgctg ggcggccttt tgggggacgg ccgctgtgcc
     2761 ggccgcgcgg cgcggcggtc ctcgccggca tcgacctgat cgaggttgcc gaggaccggt
     2821 tccgtgcggc gcgggttgcc gtttgaatcc tgccgaccgt tcatgccaat ccatcgccga
     2881 agcaggcttc ggcgtccaat ttctcgagct gttccgacgc ccttgggacc acgacggccg
     2941 tgcgccggtt ccggcacgac ggggagcgca cgcaaaacgc cgccatctta gcagtcaccc
     3001 ggaaagcacc cgcgcgcgtt gcaggagcgc cattcagccg ccggccagcc ggcgcacgtc
     3061 cgtcacgttg gccacggcgc cgagcttcgc cagcagcgcc gacaactcgc cgtagtcgcg
     3121 cacccgcagc gcgaagtgca tcgcgatctc gccgacgtcg cggtcgctgc gggtttcgga
     3181 ggcgacgatc cgcgcaccgc tgttggcaat gacattggtg acatcccgct gcaggtcgcg
     3241 ccgatcgtag ccgaccaccg cgatggagac ttcataggcg cggtccggcg cactctgcca
     3301 gcgaacctcg atcacgcgtt ccgggttgcg cgcgcgcaac cgcgccaggg ccacgcaatc
     3361 ggcgcggtgg acggtgacgc cgcggccgcg ggtgacataa cccgtgacct cgtcgccggg
     3421 caacggccgg cagcagcgcg cgaggctggt cagcaggttg ccgattccct cgaccgtgag
     3481 cgaccccggt tggcccggtg cgcgcggcgg gcgcggcggt ggcgcaggtg cggcgggcgg
     3541 cggcgcctgc gcttccagca tcgcacgcgt caactgtgcg gtggcgatct cgcccagcgc
     3601 cagcgcgacc agaaggtcgt cgcgaccctt gcgcccgagt ttcgccggca accgctcgat
     3661 gacttcgtct gggaccgcca gccggcgggc ttcgcgctcc agcaactggc ggcccgctgc
     3721 cgtgttcgcc gcgaggtcct cgcgccggaa ccacgcgcgc accttgccgc gcgcactcgc
     3781 ggtgttgaga tagccgagtt gcagggacag ccagtcgcgg ctgggcgccg gttcgcgcgc
     3841 ggtcagtatc tcgatccggt cgccgctcgc gggctggtgg gtcagcggca cgatgcggcc
     3901 attgaccttc gcgccccggc agcgatgccc gaccatcgag tgcacgtggt acgcgaagtc
     3961 gagcaccgtc gcaccggcct gcaggtcgat cacctcgccc ttcggcgtca gcaggtagac
     4021 acgttcctcc gccagctccg agcgcaattc cgccagcagt gcgctgtcgt cctcgccctc
     4081 ggcgcgcgcc tcgagcagct tgcgcatcca ggccacgcgc gcctcgtacg cgtcgtcgcc
     4141 ggacccgccc tccttgtacc gccaatgcgc ggcgacaccg agttcggcac cgcggtgcat
     4201 ctcgtcggtg cggatctgca cctcgagcgt cttgccgtcc gggccgatca ccgcggtgtg
     4261 cagcgaccgg taatggttgc ccttcgggcg cgcgatgtag tcgtcgaact cctgcgggat
     4321 gatcggccac aacgcgtgca ccaccccgag caccgcgtag cagtcggcga cactgccggt
     4381 cagcacgcgc agggcacgga tgtcgtacag ttcgccgaag ccgatcccct tgcgctgcat
     4441 cttccgccag atcgaataga tgtgcttggc gcggccgttg acggtggccg taatcccgtg
     4501 cgcggccagg gcgtcgcgca aggcggcgac gcaacgcccg atccacgctt cgcggtcggc
     4561 gcggcgctcg tcgagcagct tcgcgatacg gcggtagagc tccggttgca ggtagcggaa
     4621 cgcgaggtct tcgagttccc acttccactg cccgatgccc agccggttgg cgagcggccc
     4681 atggatgtcg cgggtcctca cggcgagctc gcggcgttgc gcttccggca gcgaggcggc
     4741 cgcgcgcatc tccgccaact gccgcgccag caacaggtac acgacccgca aatcgcggat
     4801 gatcgccagc agcagccggc gcaggccctc gttcccgcgc ttgccgtcgc gtgcggcatg
     4861 cagcgcccac acccgctcgg cttgctgctg gccttccacg agcctgcaca ccgcggccgt
     4921 gacctgcggc acggcgtcac gccaggcctc cggtcgcgcg cacgcgaccg cgtaccatgc
     4981 cgcggcggca cgggtgtcgt cgtcgcaacc gaggtcgcac agcaaggcat ccgtctgcgc
     5041 ccaaagcgcc cgttccccgg ccggcagccc atccaccggg gctgcgggcg cgcattcgga
     5101 aggtgcttgt ggaggggaat tcatgggctg cgtcgcaggg caggaatgcc ctggaggaaa
     5161 ggtcgtcgaa agcttggcag tgtagtcaat cgggcgccat tcggccctgt gcgatggcca
     5221 tgggtcactg ctatcatcgc cgctcatgcg catccgactg atgttgccgg ccacactggc
     5281 cgtgctgatc gccgccatgc ccgtcatggc gcagcaattt tcctcgctcg aggaacggat
     5341 gtccgcggcc gacttcaagg ccgccggact cgacacgctc acgccgcagc aattgcaatt
     5401 cctggacgac tggctgcgta cgcacgagca gaccaggatg gtcagcccca gcggcagtcc
     5461 ggtgttctac ccggagagcg agccgcgcga caaattcgac acccacctgg tcggccattt
     5521 cagcggctgg ggcggccaca gcgtgttcac gctcgacaat ggccaggtgt ggaagcaagc
     5581 cgaatccggc gcgtattcct gccccgatgt cgacaacccc gaggtcacga tcaagccgat
     5641 gatcctgggc agttggctga tgtacgtgca aggctgcaac cagagcgtgc gcgtcgaacg
     5701 cgtgaaatag ccccgcgtcg cccccatctg tctttcccgc cccgatacgg gcatgatgaa
     5761 ccgtgccgcg cgagcgcagg ctcgggagaa tcgcgacgtg ctggaagtct ggttgccgct
     5821 tttgggtttg atggcgctcg gcgcggcctg gtaccacgtg ctgcgcctgc gcgagcgtgc
     5881 gatcctgcac gcgcggcaac tgtgcgatcg ccacggcctg caattgctcg atgacagcgt
     5941 cgggctgcac cgcctgcacg cgcgctggcg ccacggcgcc ctgcaggtga cgcgcgaata
     6001 ccggttcgat accagcctcg gcggcgacga tcgccgcacc gcgagcatca ccctgctcgg
     6061 cgaccggatc gtggccaccc gcctgccgtc gcgggaaccg ccggccggcg cggcgcccgt
     6121 ggcaacgtcg ccgttcgtgc cggccaccct tgcgcgcggc ggcgagttgc cggacaacgt
     6181 cgtgccgctc gatcgccggc ggcggacgct gcactgaccc ggcgcccagc tacttttccg
     6241 gacacccgtg cccgcgatcg caatcgcttt ggcgcgagcg cgtcgatttc ccgcaccgat
     6301 gcacgatcga tcgagcacgc ccggcctgcc cgcgtgatcg gctgagccca cttgccgcat
     6361 caccgtggtt tgctcttcga atcgatccgg cgttcgaacc gcagccctgc cggctcgatc
     6421 ggcgtcacgc aactttgtca acctccgtaa cgcaggcgat gcaaaatacg gcgcagaact
     6481 ccagcgctgc gaacggggcg tccatccagc acgttgcggg gccagcggcg gtcgattcgt
     6541 cccgccgccg ggttggagtg catcggggtg tagtaaacgc aatcgtcgaa gtaatagtgg
     6601 gtaacctgcg accatcgggt acgcgaacgg tcgtcgtggc gggcgccgcc atgcaacagg
     6661 tttgccgtcc acacaagcgc ctggcccttg cgcgccgcga aacgctgcgg ttgcagaccg
     6721 tgcttggcca cgagcgcgtc ccacagcggc tggtacaggc gctggcccgg gtgcgggttc
     6781 aagtcggctg cttccacccc gagttggtca ttgtcgtaga tcggaagctt gtggctgccc
     6841 gggtagtaga tcaggggtcc actgtcgagt tcgacgtcct ccaacgccac ccacacaccg
     6901 cacatgaacc gctccggcat ggagtggaaa tgcacggcgt cggtatgcct cggctgctgc
     6961 gtgccgaccg ggaagttgag tgtctggaat ggccacgcgc tccgaccgta cagcagcgac
     7021 aagagttcga ggatgtgctt gttgaccgcc agcgcacgca cgtcgggatg gaatgtccag
     7081 gcgtcctgga tgcgcatgct gccgcccttc ttccgccacg cacccagatc gaagcgctcg
     7141 ccaagatcgt gctttatggc ctctgcatgg cgttcgaagt cctcgtcggg gaagtcgatc
     7201 accgcgaacc cacggtcgtg cagatcgcgc gcaacgcgca gcgtttccgc atcgggatca
     7261 agccgcgcga agatctcgtc gaaaaacgga gattcgatgc gagggacccc ggcgaggagc
     7321 cgctccggcg taaccgtgtc ggccatcacg ccgaattcgg tatgtcaggg accacgcctg
     7381 tgcccctcgg ctctacgagc acgcaagtgg ccgctgcagg gcacgaagaa ccatgagccg
     7441 ccactatttg atgacgtgca ggttgggctt gccgcggggc cgcggcttgt cgggaccgtc
     7501 gccttggccg gacgaggcgg aatctcccgg ggcgggcttg tcgcgcgaag aagcgggcac
     7561 cgcctgcagc gggctcgcgg gcctggttgg cttcgggggc tccgactctg gcggctgcgg
     7621 atcggcggcg ggtccgatcg gcgccgtcgc gatcgtcgca tcttcggcga gcagcatgcc
     7681 ctgcccgttc tcgcgcgcgt agatcgcctg gactgccgcg atcggaagcg tgaccgcgtg
     7741 gctcacgcca ccgaagcggg ccatgaaagt gatcgcgtcg tttccgatct cgagccgggc
     7801 gaccgcgcgt ggcgcgagat tcaggaccac ccgcccatcg ctcaccgact gcggcggaac
     7861 ctggatcccg ggttgcgacg cgtccaccac gacgtagggg gtaagtccgt tgtcgcagat
     7921 ccactcgtgg accgcccgca gcaggtacgg gcgattcgag gtcatggcgg gcgctggggt
     7981 cacaactcgc ccagcagacg ctcgtccgag gtaaggctgc gcgcgaagcc ctggctgcgg
     8041 aacagccgct cgccgtaatc gatgatcggc ttggcttccc tcggcagcga aaccccgagc
     8101 gacggcagcc tccataccac cggcgccacc aggcagtcgg caaggctgat ctcgggattg
     8161 aggaagaact tggcggcctt gaacagtgga agtgccgtca tgaggtgctc gcgcaagcgc
     8221 ttgcgtgccg gttcggcggt gcggccgccg gccttgatcg aagccacttc gggaacccag
     8281 tcgcgctcga tccgcacgca ggcgagccgc aggcgagcac gcgacagcgg atcgaccggc
     8341 atcagcggcg gatgcgggta gcgttcgtcg atgtactcgc aaacgacgtc gggctcgtac
     8401 aggacgaggt cgcgatcctg cagcgtcggc gtgtcaccgt agggattgat ggagagcaat
     8461 tcgtcgtgcg gcttgccggc cggcacctcg acgcggtcgt acaccacccc cttggcggcc
     8521 agcacgagcc ggacgcggtg cgactgcacg tcatccggcg cggtgaacag ggtcaatacg
     8581 gtgcgggtgt gggcggtttg gatcatgcca gtgtcccctg tgggccttgt gggtccggtg
     8641 acgcgaccgt cccggctctg gtggcatcgc gttcccggct acgcttgccg gctgtcaatg
     8701 cacgtccttc cagtattcct tcttcagcac cagcgcgaac agggtgaaaa gtaccagata
     8761 aatcacgacc caggcgccca tccggctgcg caccagttcg tccggggagg agacgtacgc
     8821 caggaacgcg gtgaggtcgc gcaccgcgcg gtcgtactgg gcaggggtca tgctgccggg
     8881 ctgcgcgagc ttgagctcct tcacggtgcc cggctggccg ttcgcgggcg gattcatcac
     8941 cgccacctgc tcgccctgca actgccacag cgggaacggc atcgcgacgt tcgggaacac
     9001 ggtgttgttc cagccgctgg tcgtggaagg atcgaggtag aacgacttca tgtaagcggc
     9061 cacccagtcg gcgcccttcg cgctgacttc cagcgacagg tccggcgggg ccttgccgaa
     9121 ccatttttcg gcatcggctt ccggcatcgc cgagacgatc ggctcgccga acttggcgcc
     9181 cgtgaagttc aggttgtcca tcacctgctt ttcggtgagc cccagtccct cggccatctg
     9241 cgaatagcgc atgtatttca gcgaatggca gccggcgcag taattcatga acagacgtgc
     9301 gccgcgctgc agcgcgcctt ggtcgtgcac gttgacgttg gcgttcggga agccgccttc
     9361 ctcggcgaag gcggcgggcg ccgcaagcgc caccagcaac gccagcagca ccgcggtcag
     9421 cgcgcgcatg gaagcgcaag aagtcttgtc atgcatgcgt cgtcaccctt tccggaaccg
     9481 acctggtcct ctccagcccg aagaacgtgt aaacccagag gaacacgaag aacgcgaaat
     9541 agaggatcac gaggatccgc ccgaacaggt tctcgatggt cgtgatgtcg gcgtcggggc
     9601 cgaagatcgc gacgatccag ccggccgtca tgtccatgcc gatgcacccg agcagcacga
     9661 acacgatcgc gaagatggcg agggcgatct tgtaggcaag tccgcggtag cggatcgact
     9721 tgaccccgcc gcggtcgagc cagggcacca ggaacagcac cacgatcgaa gccgccagcg
     9781 cgaggacccc gagccccttg ctcgggatca tgcgcaggat cgcgtagaac ggcgtgaagt
     9841 accacaccgg cttgatcgtg gccggggtca ccaggtcatt cgcgggaatc gagttgtcgt
     9901 gctcgaggaa gaagcccccg acgctcggcg cgaagaagat gatgaccgcg cagatcacca
     9961 ggaacacgcc cacgccgacc aggtccttga ccgtgtagta cgggtggaac gggatgccgt
    10021 ccagcgggat gcccttgggg ttcttgtgct tcttgatctc gaccccgtcg ggattgttgg
    10081 agccgacctc gtgcagggcg ccgaggtgca ggaccaccag cagcagcagc accagtggca
    10141 gcgccaccac gtgcagcgcg aagaaccggt tcagggtcgg atcgcccggc acgaaggtgc
    10201 ccatgatcca ggtcaccagc gtgtggccga accacggcac cgcgctgaac agggtaatga
    10261 tcaccttggc tccccagaac gacatgttgc cccacggcag cacgtagccc atgaaggctt
    10321 cggccatcag caccaggaag atcagcatgc cgaccagcca gaccagttcg cgcggcttct
    10381 tgaacgagcc gtacatcagc ccgcggaaca tgtgcaggta gaccacgatg aagaacatcg
    10441 aggcgccggt cgtgtgcatg tagcggacca gccagcccca gttgacgtcg cgcatgatgt
    10501 actgcaccga gtcgaacgcg ccggcggcgg tcggggtgta gaacatcgtc aggaagatgc
    10561 ccgtgacgat ctggttcacc agcacc
//
LOCUS       DASSNH010000304        18168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1599383, whole genome shotgun sequence.
ACCESSION   DASSNH010000304 DASSNH010000000
VERSION     DASSNH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 18168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18168
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599383"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFK00_11355"
     CDS             complement(<1..432)
                     /locus_tag="VFK00_11355"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344297.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7593584.1"
                     /translation="MPEELKQAALEYHRHPPAGKIRITPTKSLLTARDLALAYSPGVA
                     AACEEIVRDPLEAHALTARGNLVGVITNGTAVLGLGSIGPLAGKPVMEGKAMLFRKFA
                     GIDVFDIEIDERDPDKLVEIIASLEPTFGGINLEDIKAPECF"
     gene            577..2256
                     /locus_tag="VFK00_11360"
     CDS             577..2256
                     /locus_tag="VFK00_11360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7593585.1"
                     /translation="MRAGSWRWVLLLLWMPVAAWAGDAAVRVIPLRAAPALAAGQAPS
                     AGKGTTRSHVWLRAPRASTAWYELRLAHDWHRSSPPVLAISGNTRARVTALLPPDYAP
                     HSETIFDTDLDPTFSHHAVVYPLPSGLRADQPVYLALGSPGQTQPIRVRITDEQDYRI
                     GDLAHVRVSTFFASVQVSMVLVILCFWIVLRDRMFLYFVAYVGAQVIYGMAVSGELFA
                     MPGAGLLTAHGYHTGQSAATLAAAFSIWFILEFADLGRDTRRLAAWLGALRWPFLALT
                     AIVWMPWLRPDEWLPNTVNVLLIVSTTIALASSWLAWRRGNRQAGFFLLSWVPLLVLT
                     VTRVVQLIGGFPLPAWLEYGFPGSMAWAAVIITVGLADRTLQVRHERDHATRMAQFDA
                     LTGAYNRRTIMDRLQEDWRMAVMTSRPLAVLFLDIDHFKQINDSHGHAAGDTCLREVT
                     EAIGMELGRADRMGRYGGEEFLIVLNGHSATIAAQVAQRTVARIAALRVPIGERQVAL
                     TVSVGTALRDARVTSAEALVECADVALYRAKAEGRNRAVTYCRDPVEPALA"
     gene            2301..3086
                     /locus_tag="VFK00_11365"
     CDS             2301..3086
                     /locus_tag="VFK00_11365"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HET7593586.1"
                     /translation="MQDKLDIVRDWLPRYTGERLENFGRHILLTNFIGYVETFCALTG
                     ASIVDRERPMPCATADGLTMIDFGMGSPNAATVMDLLSAIAPEAVLFLGKCGGLKRKN
                     RLGDLILPIAAIRGEGTSDDYLPREVPALPAFQLQRGVSTMIRDLGHDYWTGTVYTTN
                     RRVWEFDAGFKDYLRTLRCMAIDMETATIFAAGFANRIPSGALLLVSDQPMVPEGVKT
                     SVSDARVKAGFVAEHVRIGFEALRLVRRNGRSVKHLRFEDELE"
     gene            3217..3666
                     /locus_tag="VFK00_11370"
     CDS             3217..3666
                     /locus_tag="VFK00_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593587.1"
                     /translation="MATFNILLAIHVLGVVWWVGGVAMVTAVLLPIFNRLPPPERIQR
                     IKQLEARFANQARVAVVLVGITGFWMLGITGGFARLQFAYGWWIDLMLLVWVLFVVML
                     FVAEPLRLPAKIGLIHKPGAFLGVHAVLLALALAAIFFGVIGARGGF"
     gene            complement(3721..5568)
                     /locus_tag="VFK00_11375"
     CDS             complement(3721..5568)
                     /locus_tag="VFK00_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7593588.1"
                     /translation="MELTLTPDMMLVLGLVGFTVLMLILEWVRADIVALLVVVVIGIT
                     GLMPVDQLFNGFASNAVLSLIGVMVMGAGLDRTGVLNRAASFILRISGGVESRVSLAL
                     NATVGSLSSIIQSQALAALFLPVASRISARTGVPLKRLLLPMACCILAGTSMTLISNS
                     PLILLNDLIASANRNLPPGAQTIEPFSLFSITPIGIPLLLVGILYFALFTRRVMPDEV
                     EDKPKVTPGRTESYFADTYGIEGEVIELTVTADSPLVGMSIGEAEVLDKAPLILAIKN
                     GNETRLSPPSDQMIWVGTVLGVLGTRADVSAFAQDMQCRVGNRVQALADIFNPTRAGI
                     SEVVIPPLSKFIKQEVGELRLRKRYGISVLAVNRGDEVIRGEDVRRLTLRAGDSLVVH
                     SYWRDLALAAEGRDLVPVTDIPTEEQRPGKVRYALAFFALAMALGLFTDLRLAVAMLT
                     GAVGMLLAGVLNMDEAYGAINWKTIFVMACLIPLGISMDSTGTAAWMAQEILAYLGGL
                     PQWVIQAFVAILALIFSQIISHVGTAVMMVPMAINIALASGGNPSVYALIVALSVSNT
                     FLIPGASPTLLIVAGPGGYRPRDFLRVGLPLTAVMLVVTLLMVNVVFGR"
     gene            complement(5741..6457)
                     /gene="trmB"
                     /locus_tag="VFK00_11380"
     CDS             complement(5741..6457)
                     /gene="trmB"
                     /locus_tag="VFK00_11380"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7593589.1"
                     /translation="MTDSQGPDHRRTVRSFVLREGRITPAQQRAFEQHWQRFGVDYDG
                     QKRDLDALFGRRAPRVLEIGFGNGEPLAWAAEHDPARDYLGIEVHRPGVGRLMNALAA
                     LDAGNVRIWNHDAVEVLQNELADGALDEVRIWFPDPWPKKRHHKRRLIQPAFAALLAR
                     NVKPGGLLHLATDWPGYALHMREVLAGHAGWRLREGESGESRRPPWRIETRFERRGVA
                     LGHPVTDLLYDRVPSSPHAA"
     gene            complement(6552..7367)
                     /locus_tag="VFK00_11385"
     CDS             complement(6552..7367)
                     /locus_tag="VFK00_11385"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET7593590.1"
                     /translation="MNAQPQPSASNDPPDSLVIAGRAYGSRLLTGTGKFKDLDETRRA
                     SEAAGSEIVTFAVRRMNLGQKADEPNLLDALPPERFTLLPNTAGCHSADEALRTCRLA
                     RELLDGHSLVKLEVIGDQKTLFPDVIETLAAAEVLVRDGFDVMVYTTDDPLLAKRFEE
                     LGCAAVMPLAAPIGSGLGIQNRYTILEIVENARIPVIVDAGVGTASDAALAMELGCDG
                     VLMNTAIAHAQDPVLMAHAMKCAIEAGRCAFRAGRIPRKRYASASSPVDGMIG"
     gene            complement(7634..7834)
                     /gene="thiS"
                     /locus_tag="VFK00_11390"
     CDS             complement(7634..7834)
                     /gene="thiS"
                     /locus_tag="VFK00_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET7593591.1"
                     /translation="MQITLNGQPRECEPATTVAALLAESGHGERRVAVEVNREIVPRS
                     QHAQHRLHDGDQVEIVQAIGGG"
     gene            complement(8012..10474)
                     /locus_tag="VFK00_11395"
     CDS             complement(8012..10474)
                     /locus_tag="VFK00_11395"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET7593592.1"
                     /translation="MSLRCLLRPLLVTGCTCLALLTFAATAPAQQYNPLNGPVMDQDA
                     GQAPGRVARLAWLSGQVQFAPAGEDDWGSVEVNRPMVIGDRLLTGDDGRAVLELGDAS
                     VRIDHGSAFDFLNLDEGNVQIELSQGTLNLAVRDLGDNENFEVDTPTVAFVATAPGIY
                     RIDNSPTGTGSMVTVFQGSGVVYGENGASREVQAGTSYRFDDSTLASVEANGLPEPDD
                     FDRFCETRDTNYTHYAEQQRQYIPSDMIGGDDLYRYGQWNAVPEYGNVWYPRSVPVGW
                     APYRYGHWAWIDPWGWTWVDNQPWGFAPFHYGRWVYVQNRWGWMPGPVNVRPVYAPAL
                     VAFVGGSGVSLSIRLGGGGPVGWFPLGPRDVYVPWFHASRGYFTNVNVTNIRNVYVNR
                     VVINNFYDDYRHQRYADIHRRNHYRYRELPGAVTMVPRTIFANARPVHPAALKLDPKQ
                     LRNAQLSVRPDVNPGKASIGLHRPAARPAVAHEREAFARPVVARHAPPPRPVGFAARE
                     KLIARQHGQPLTTEQMRNLRKAQPAHAADEQRVKLVASNGKASRPAPVEAVRARRATA
                     TQPAHRDVSARTVKPSGDVAGDAHPAPSRNTAHAAPARPQPTRDASLRAGELPSARFR
                     HQPQQATLPRAVAIRPATKDEQQSRAPAGRLPVVQPVKPASRPQPRADNPAQPPRARI
                     ERPQPQPQPQKLRQEQAEQQARAQQARQRSAAAEQQGAQQQQMERHRAAQAQMQQQQR
                     EARMRQPQPVRAPQLQQQRQVQQMRTQQAQQAQREGRQHSVQQPQERPAPRAATRPPA
                     KQHPQSPPKKHPDSSSDHQRIR"
     gene            10666..10736
                     /locus_tag="VFK00_11400"
     tRNA            10666..10736
                     /locus_tag="VFK00_11400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10698..10700,aa:Gly,seq:ccc)
     gene            10749..11213
                     /locus_tag="VFK00_11405"
     CDS             10749..11213
                     /locus_tag="VFK00_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET7593593.1"
                     /translation="MVDTRGALPVLGWRERVALPRLGIGRIKAKLDTGARSSALHVDS
                     LESFRRDGDDWLRFDLATGRRPPRRVVCEAPALDRRRVTDSSGNASVRWFIETEVLLG
                     TLALCIEVSLTDRRGMLFPMLLGRTALAGRALVDPARSYTLSLSTATPPPTR"
     gene            11210..12085
                     /gene="rimK"
                     /locus_tag="VFK00_11410"
     CDS             11210..12085
                     /gene="rimK"
                     /locus_tag="VFK00_11410"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HET7593594.1"
                     /translation="MKLAILSRNARLYSTMRLLAAARARGHKARVLDPLRCYMRISHD
                     GFGVHYGGRQLKGYDCVIPRIGASVTFYGTAVLRQFEMMGVYTPSPSEAVLRARDKLR
                     SLQMLASHGIDLPATVFGDNPDDTGDLLAMLGPPPHVIKLNEGAQGTGVVLAEKLPAS
                     QSVIEAFRGLYANFLVQEFVREANGRDIRCFVVGGKVVAAMERAAVEGDFRANLHRGG
                     TGSPVKLESEERRTALRAAKVLGLGIAGVDLLRSERGPLLLEVNASPGLEGIEKVTGV
                     DVADAVIRHLEAALE"
     gene            12379..12573
                     /locus_tag="VFK00_11415"
     CDS             12379..12573
                     /locus_tag="VFK00_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593595.1"
                     /translation="MLPGGRWQDVSDSERAEHSASFARMEKPFDEHEVVDDMGQHARL
                     TLLDAQNDVNVILPTTTAIA"
     gene            12943..13512
                     /locus_tag="VFK00_11420"
     CDS             12943..13512
                     /locus_tag="VFK00_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593596.1"
                     /translation="MKTSSGLILAAAIGALMLQGCAATGETRASAAPTAPPASQAAPM
                     AAAPAAQVPEEDGKPVVKADTAENFAAVVAAIHQQMQPGGRWQYVDSRERATINGSFG
                     DMQSLYDQFGSVDKMDQAARMRLLADQNTVNAILTQKDGDRLVCENRMPVGSHLPVKT
                     CKTYAQMQAEQRGAQEFMRQREGTMQQRH"
     gene            13688..14089
                     /locus_tag="VFK00_11425"
     CDS             13688..14089
                     /locus_tag="VFK00_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET7593597.1"
                     /translation="MMVARERKLPRPTTAELEILQVLWEHGACTVREVHEELHRRDRT
                     GYTTALKQLQIMFDKGLVLRNESRRAHVYRAAVSKERTQKKFLSDMLQRVFDGSPSRL
                     VLQALGDHKTSRAELNEIRALLNRLDKEAEP"
     gene            14086..15774
                     /locus_tag="VFK00_11430"
     CDS             14086..15774
                     /locus_tag="VFK00_11430"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7593598.1"
                     /translation="MNVAGIFSIGVVALGWSLLHFVWQATLVGIVYAVTRGMLPRGNP
                     RYLAAMLAMMALAIWPLVTAWHEAASYMGTVGLPGAVVSAAPTRASASAAESPGWQAW
                     FDAMMPWLVLAWAAGVGVLGTRVYRQCRGLRSILRAAGVQPEWQARARAIADRMRLHR
                     VVPVLVSLRVGTPTLIGWLRPAVVLPVAVLARMPAAQVEMVLAHELAHLKRLDPLANL
                     FQVVLETLFFYHPVVHWISRDARHERELCCDALALRATGGQRRDFVAALAVLAEFRSS
                     HADLALAASGGALAERAWFIAGDAPEPRHRTGGLAFAGLALLGLAMVFGVAWRQEIRS
                     QRVEDMLAGGVSALRQSLGGIAGSPLPLGYQLPDQRPRLAPVSLAALVAPTPTLAPIA
                     VPSAKKAGVASPRVAVQDLAPPPAGVHPVAVASPLPASAGGSSDSTVQPRAIRAPQPE
                     YPAQAMYSGVQGRVEIQFTLSAAGVPRGMRVVGTSGSTSLDGAALDALARWRFTPPGV
                     AGRQYRQTFNFELEPGAAAAGMAGSQGCVRSTGTHICRQPIDEAAAVRTPGPGR"
     gene            15860..16546
                     /locus_tag="VFK00_11435"
     CDS             15860..16546
                     /locus_tag="VFK00_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7593599.1"
                     /translation="MRVLVIEDNNDIATNIGDYLEDRGHVVDFAGDGVTGLHLAVVND
                     FDVIVLDLTLPGMDGIEVCKKLRGEAHKHTPVLMLTARDALEQKLTGFESGADDYMTK
                     PFALQELAARLEVLARRGKGPTSRVLKIADLTYNLDTLVVTRAGHSIQLNPIGLKLLQ
                     ALMEASPSVVTRQDLEQRVWGEELPDSDSLRVHIHGLRAAIDKPYGKPLIHTRHGIGY
                     RMVDPDAVPA"
     gene            16530..17822
                     /locus_tag="VFK00_11440"
     CDS             16530..17822
                     /locus_tag="VFK00_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7593600.1"
                     /translation="MQSRRKLRVRLIVTFTLFGFCLSALFAVASLYVRAKVENQLIDQ
                     SLEADLRQAVENKHLHPDQPLQSKLIVVWLWNDRTVYQAPLAWQGLPPGVYDMRGGDN
                     GLPQHYKLAVVRKYGIDAFLTYDISREGLGKRQLLTSLVGAVVLFTLLSLAVGYWLSR
                     RVMRPVTELAQRLRAFRSGRQLESLAPHFAEDEVGELAAALDDYAQRQSALIERDREF
                     NSDVSHELRTPLAVIASTTELLVATPELPEKLRTRLQRIERAVRQATELTQALLLLSR
                     AERSGPVDGETTDVAKVVLEVVDTHRPDLGRKPIELRTEIEAPLTVEAPSSVVAVALG
                     NLVGNACKYTQEGEIVVRVQRGRVRVEDTGPGIKREDAERLFERGVRGEGETVKGAGL
                     GLAIVRRLCELYGWQVALAPRAERGAVATLDFGDVGEA"
     gene            18045..>18168
                     /locus_tag="VFK00_11445"
     CDS             18045..>18168
                     /locus_tag="VFK00_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HET7593601.1"
                     /translation="MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDIRRINLPA"
BASE COUNT         2788 a         5970 c         6381 g         3029 t
ORIGIN      
        1 gaagcattcc ggcgccttga tgtcctcgag gttgatgccg ccgaaggtcg gttccagcga
       61 ggcgatgatc tcgaccagct tgtcggggtc gcgctcgtcg atctcgatgt cgaacacgtc
      121 gatgccggcg aacttgcgga acagcatcgc cttgccctcc atcaccggct tgccggcgag
      181 cggaccgatg ctgccgaggc cgagcaccgc ggtaccgttg gtgatcacgc ccaccaggtt
      241 cccgcgcgcc gtgagcgcgt gggcctcgag cggatcgcgc acgatttcct cgcaggccgc
      301 ggcgacgccg ggcgaatacg ccagggcgag gtcgcgcgcg gtcaggagcg acttggtggg
      361 ggtgatgcgg atcttgccgg cgggcgggtg ccggtggtat tccaacgcgg cttgcttgag
      421 ttcttctggc aggttggaca tggcgaccgg gacacgcgcc tggacggatc gggacctttc
      481 atcctaacac ttcgccggcg cggccagccc gtgccggcgc gacgaaaacc cgttacagtc
      541 gccgcgagat gccaagggcg gccggggggt cgaagcatgc gcgcaggatc atggcgctgg
      601 gtgctgctgt tgctctggat gccggttgcc gcatgggccg gtgatgccgc cgtgcgcgtg
      661 atcccgctgc gggcggcgcc cgcgctggca gcgggccagg cgccttcggc agggaagggc
      721 acgacgcgct cgcacgtatg gttgcgtgcg ccccgcgcga gcaccgcttg gtatgaactg
      781 cgcctcgccc acgactggca tcgttcgagc ccgccggtcc tcgcgatctc gggcaacacc
      841 cgcgcccgcg tgacggcatt gttgccgccc gactacgcgc cgcacagcga gaccattttc
      901 gacacggacc tcgatcccac gttctcgcac cacgccgtgg tgtatccgtt gccatcgggc
      961 ttgcgcgcgg accagcccgt ttatctcgcc ctcggcagcc ccggccagac ccagccgatc
     1021 cgcgtgcgca tcaccgacga acaggattac cgcatcggcg acctggcgca cgtgcgggtc
     1081 agcaccttct tcgccagcgt gcaggtgtcg atggtgctgg tgatcctgtg cttctggatc
     1141 gtgctgcgcg accggatgtt cctgtatttc gtcgcctatg tgggcgccca ggtgatctac
     1201 ggcatggcgg tgtcgggcga gttgttcgcg atgcccggcg cggggctgct gacggcgcac
     1261 ggctaccaca ccggacagtc ggccgcgacg ctcgcggcgg cgttctcgat ctggttcatc
     1321 ctcgagttcg cggacctcgg ccgcgatacg cggcggctgg ccgcatggct gggtgcgctg
     1381 cgctggccgt tcctcgcgct gacggcgatc gtgtggatgc cgtggctgcg ccccgacgaa
     1441 tggttgccga ataccgtcaa cgtgttgttg atcgtgagca cgaccatcgc cctggcttcg
     1501 agctggctcg cctggcggcg cggcaaccgg caagcggggt tcttcctgtt gtcgtgggta
     1561 ccgctgctgg tcctgacggt gacgcgggtg gtgcaattga tcggcggctt cccgctgccg
     1621 gcatggctgg agtacgggtt cccgggcagc atggcctggg cggcggtgat catcacggta
     1681 ggcctggccg accggaccct gcaggtccgg cacgaacgcg accatgccac ccgcatggcg
     1741 cagttcgacg cgctcaccgg cgcgtacaac cgccgcacga tcatggaccg cttgcaggaa
     1801 gactggcgga tggccgtgat gacgtcgcgg ccgctagcgg tgctgttcct ggacatcgac
     1861 cacttcaagc agatcaacga cagccacggc cacgcggcgg gcgatacgtg cctgcgcgaa
     1921 gtgaccgagg cgatcgggat ggaactcggc cgcgccgacc gcatggggcg ctatggtggc
     1981 gaagagttcc ttatcgtgtt gaacggacac agcgcgacca tcgccgcgca ggtggcgcaa
     2041 cgcacggtgg cgcggatcgc ggcgctgcgg gtgccgatcg gcgagcgcca ggtcgcgctc
     2101 accgtcagcg tcggcacggc cctgcgcgat gcgcgggtga ccagcgccga ggcgctggtg
     2161 gaatgcgcgg acgtggcgct ctatcgcgcc aaggccgagg gccgcaacag ggcggtgacg
     2221 tattgccgcg atcccgtcga gcccgcgctc gcgtgagtgc acgcgcgccc gatggcgcgg
     2281 cggctggcac actggccgtc atgcaggaca agctggatat cgtccgtgac tggctgccgc
     2341 gctacaccgg cgagcgcctg gaaaacttcg gccgccacat cctgctgacc aacttcatcg
     2401 gctacgtcga gacgttctgc gcgctcaccg gcgcgagcat cgtggaccgc gagcggccga
     2461 tgccgtgcgc gaccgcagat gggctgacga tgatcgattt cggcatgggc agccccaacg
     2521 ccgcgaccgt gatggacctg ttgtcggcga tcgcgcccga ggccgtgctg ttcctcggca
     2581 agtgcggcgg cctcaagcgc aagaaccggc tcggcgacct gatcctgccg atcgccgcga
     2641 tccgcggtga gggcaccagc gacgattacc tgccgcgcga ggtgccggcg ctgccggcat
     2701 tccagttgca gcgcggcgtc tcgacgatga tccgcgacct cggacacgac tactggaccg
     2761 gcaccgtata caccaccaac cgccgggttt gggaattcga tgccgggttc aaggattacc
     2821 tgcgcaccct gcgctgcatg gcgatcgaca tggaaaccgc gaccatcttc gcggccggct
     2881 tcgccaaccg cattccatcg ggcgcgttgc tgctggtgtc cgaccagccg atggtgccgg
     2941 agggcgtcaa gaccagtgtg tccgacgcgc gcgtcaaggc cgggttcgtc gccgagcacg
     3001 tgcgcatcgg cttcgaggcg ctccgtttgg tgcgccgcaa cgggcgcagc gtcaagcacc
     3061 tgcgcttcga agacgagctg gaatgacctg aggcatccgc ggtccgggct cgaaccgcgc
     3121 tcgaccgagc ggaagctgca ttgacttgcg tcatgggccc ccggccgggt gcgcctagac
     3181 tggcgacacg atttggtcgg tgagccgcgc gagaccatgg cgacgttcaa catcctgctg
     3241 gcgatccacg tgctgggcgt ggtttggtgg gtcggcgggg tcgcgatggt gacggcggtg
     3301 ctgttgccga tcttcaaccg gctgcccccg cccgaacgca tccagcggat caagcaattg
     3361 gaggcgcgct tcgcgaacca ggcacgggtc gcggtggtgc tggtcggcat cacgggtttc
     3421 tggatgctcg gcatcaccgg cggcttcgcg cggctgcagt tcgcctacgg ctggtggatc
     3481 gacctgatgc tgctggtctg ggtgctgttc gtggtgatgc tgttcgtcgc cgaacccctg
     3541 cgcctgccgg caaagatcgg tttgatccac aaacccggcg cattcctcgg cgtgcatgcc
     3601 gtgctgctgg cgctcgcgct ggctgccatc ttctttggcg tgatcggggc gcgcggcggc
     3661 ttctgagccg ccctctttgc cacaccgcct ggtgctcgcg gatgatcgga aaaagatgtt
     3721 ttatcttccg aacaccacgt tcaccatcaa cagcgtcacc accagcatga ctgcggtcag
     3781 cggcaggccg acgcgaagga aatcacgcgg ccggtagccg ccgggtcccg cgacgatcag
     3841 cagcgtcggg ctggcgcccg gaatcaggaa ggtgttggag accgacagcg cgacgatcag
     3901 cgcgtagacg ctgggattgc cgcccgaggc cagcgcgatg ttgatcgcca tcggcaccat
     3961 catcaccgcg gtgccgacgt gcgagatgat ctgcgagaag atcagggcca ggattgcgac
     4021 gaaggcctgg atcacccact gcggcaggcc gcccaggtag gccaggatct cctgcgccat
     4081 ccaggccgcg gtgccggtcg agtccatcga gatgccgagc gggatcaggc aggccatcac
     4141 gaagatggtc ttccagttga tcgcgccgta ggcttcgtcc atgttgagca cgccggccag
     4201 cagcatgccg accgcgccgg tcagcatcgc gaccgcgagc cgcaggtcgg tgaacaggcc
     4261 gagtgccatc gccagcgcga agaacgccag cgcatagcgc accttgccgg ggcgctgttc
     4321 ctcggtcggg atatcggtga ccgggactag gtcgcggcct tccgccgcca gtgcaaggtc
     4381 gcgccagtag ctgtgcacca ccagcgagtc gccggcgcgc agcgtgagcc tgcgcacgtc
     4441 ctcgccgcgg atgacctcgt cgccgcggtt cacggcgagt accgaaatcc cgtagcgctt
     4501 gcgcaggcgc agttcgccga cctcctgctt gatgaacttc gacagcggtg ggattacgac
     4561 ctcggaaatt cccgcacggg tcggattgaa gatgtccgcg agcgcctgga cccggttgcc
     4621 cacgcggcat tgcatgtcct gggcgaaggc gctgacgtcg gcccgggtgc cgagcacgcc
     4681 gagcaccgtg ccgacccaga tcatctggtc cgaaggcggc gacagacgcg tctcgttgcc
     4741 gttcttgatc gcgaggatca gcggcgcctt gtcgagcacc tcggcttcgc cgatgctcat
     4801 cccgaccagt gggctgtcgg ccgtcaccgt cagctcgatc acctcgccct cgatcccgta
     4861 ggtgtcggcg aagtaacttt ccgtgcggcc gggcgtgacc ttgggtttgt cctcgacctc
     4921 gtcgggcatc acgcgacggg tgaacaacgc gaagtacagg atgcccacca gcagcagcgg
     4981 gatgccgatc ggcgtgatcg aaaacagcga gaacggttcg atcgtctgcg cgccgggcgg
     5041 caggttgcgg ttggcgctcg cgatcaggtc gttcagaagg atcagcggcg agttggaaat
     5101 cagcgtcatg ctggtgccgg cgaggatgca gcaggccatc ggcagcagca ggcgtttcag
     5161 tggtacgccg gtgcgcgcgg agatgcggct cgccaccggc aggaacagcg cggccagcgc
     5221 ctggctctgg atgatcgagg acaggctgcc caccgtcgcg ttgagcgcga gcgacacgcg
     5281 gctttcgacg ccgcccgaaa tccgcaggat gaaggaggcc gcgcggttca gcacgccggt
     5341 gcggtccagc cccgcgccca tcaccatcac gccgatcagc gacagcaccg cgttggaggc
     5401 gaagccgttg aacaactggt cgaccggcat caatccggtg atgccgatca ccaccaccac
     5461 cagcagtgcg acgatgtccg cgcgcaccca ttccaggatc agcatcagca ccgtgaagcc
     5521 gaccaacccg agcaccagca tcatgtcggg ggtgagggtc agctccatcg gactcggctt
     5581 cgtcggtcag cgggttggca gtggcggcat gcggggttcc cggttgacga ccgcgaacgc
     5641 gcgcgggttg cgcgcagaca tcgcgcagcg ggcgaagtgt acacgcgggt tctccaggta
     5701 ttgcgggcac ctccctgtgc gcggcgccga ctgcgccggg tcaggcggca tgcggcgatg
     5761 atggcacacg gtcgtacaac agatccgtaa ccggatgccc gagcgcgacg cctcgtcgct
     5821 cgaagcgcgt ctcgatccgc catggcggtc gcctggattc accgctttcc ccctcgcgca
     5881 gtcgccagcc cgcgtggccg gccagcactt cccgcatgtg caaagcatac ccgggccagt
     5941 cggtcgcaag atgcaacaga cctcccggtt tgacgttgcg cgcgaggagg gcggcgaacg
     6001 cgggctggat cagcctgcgc ttgtggtggc gcttcttggg ccacggatcg gggaaccaga
     6061 tccgcacctc gtccagcgcg ccatccgcga gttcgttctg cagcacctcg acggcgtcgt
     6121 ggttccagat gcggacgttg ccggcgtcca gcgcggcgag cgcgttcatc agccgcccga
     6181 caccgggacg gtgcacttcg atgccgaggt agtcgcgcgc gggatcgtgt tcggcggccc
     6241 aggccagcgg ctcgccgttg ccgaagccta tttcgaggac gcgcggcgcg cgccgtccga
     6301 acagtgcgtc gagatcgcgc ttctggccgt cgtagtccac gccgaagcgt tgccagtgct
     6361 gctcgaacgc ccgctgttgc gcgggggtga tgcggccctc ccgcagtacg aagctgcgga
     6421 cggtccggcg atggtcaggg ccttggctgt cggtcatcgc gctgttcttt ccctctccgc
     6481 tcgggagagg ctggcgcgaa gcgccgggta aggtcacggt tcccgcgcaa aagccgcacc
     6541 agagaaggct cttagccgat catgccgtcg accgggctgg atgcggacgc gtagcgcttg
     6601 cgcgggatac gccctgcgcg gaatgcgcag cgaccggctt cgatggcgca cttcatcgcg
     6661 tgcgccatca ataccgggtc ctgcgcgtgc gcaatggcag tgttcatcaa tacgccgtcg
     6721 cagccgagtt ccatcgccag cgcggcgtcc gatgcggtgc cgacgccggc atcgacgatc
     6781 accgggatcc tggcgttctc gacgatctcg aggatggtgt agcggttctg gatcccgagc
     6841 ccggaaccga tcggtgcggc gagcggcatc accgcggcgc agcccagctc ctcgaaccgc
     6901 ttcgcgagca gcggatcgtc ggtggtgtag accatcacgt cgaagccatc cctcaccagc
     6961 acttcggcgg ccgcgagcgt ctcgatgacg tcggggaaca gggtcttctg gtccccgatc
     7021 acctcgagct tcaccagcga atgaccgtcg agaagttcgc gcgcgagccg acaggtacgc
     7081 agggcttcgt cggcgctgtg gcagccggcg gtgttcggaa gcagggtgaa gcgttccggc
     7141 ggcagcgcgt cgagcaggtt cggttcgtcc gccttctgtc cgagattcat ccgccgcacc
     7201 gcgaacgtga cgatctccga gccggcggct tcgctcgcgc ggcgggtttc gtcgagatcc
     7261 ttgaatttgc cggtgccggt aaggaggcgc gaaccatagg cacggccggc gatcacgagc
     7321 gagtcgggcg ggtcgttgct ggcggagggt tgcggttggg cgttcatggc ggtcggactg
     7381 cagtcggaac gaactgcaat tctactagcg ccgctggcga acccatcttc cgtcattccc
     7441 gcgacagcgg gaatcgagtc acccagagcg gtgtcccatg aaaacattcg tcattccgga
     7501 cgaacccacg gagtgggttg gatccggaat cggccttcat ttgttttttg aatacggcag
     7561 attccgggtt ccctcgccgg caagccggcg cgagccccgg aatgacgaca aagggattct
     7621 tcgcgacacc acgctacccg cctccaatcg cctgcacgat ctcgacctgg tcgccgtcgt
     7681 ggagccggtg ttgcgcgtgc tggctgcgcg ggacgatctc gcggttgact tcgacggcga
     7741 ccctgcgctc gccgtggccg gattccgcga gcagggcggc gacggtggtt gccggctcgc
     7801 attcgcgcgg ttggccgttg agcgtgattt gcatgtggtc attgtagcca gcgcaagcgc
     7861 cagctcccga agcggttttg cggaaagtcg aagccacccc cgagacgcgt ccgatcgggc
     7921 ccgggaaggc cgttctggtg tccacggggc caacagccga cctcaaaaat ggtcggctgc
     7981 agtgggcggc gatcacggac gatcgcaaaa gctaacgtat ccgttggtgg tcgctgctgc
     8041 tgtcagggtg cttcttcggt ggcgattgcg gatgctgctt ggccggtggg cgagtggcgg
     8101 ctcgcggcgc agggcgttcc tgcggctgtt gcacggaatg ctgacgcccc tcgcgctgcg
     8161 cctgttgcgc ctgctgcgta cgcatctgct gcacctgccg ttgctgttgc agctgtggag
     8221 cgcgtaccgg ttgtggctgg cgcatccgcg cttcgcgttg ttgttgttgc atctgcgctt
     8281 gcgccgcacg atgccgctcc atttgctgct gttgcgcacc ttgctgctcg gcggcagcgc
     8341 ttcgctgccg ggcctgttgc gcccgtgcct gctgctcggc ctgttcctgc cgcagcttct
     8401 gcggttgcgg ttgcggttgc ggccgctcga tgcgcgcgcg gggcggctgc gcggggttgt
     8461 cggcccgcgg ctgcggacgc gacgcaggct tgaccggctg caccaccggc agccgccccg
     8521 ccggcgcacg cgactgctgc tcatctttcg ttgcggggcg aatggcgacg gctcgcggca
     8581 gcgtcgcctg ctgcggctgg tgccggaacc gcgcggatgg cagttcgcca gcacgcaacg
     8641 aggcatcgcg ggtgggctgt ggccgcgcag gggccgcgtg cgccgtgttc cgcgagggag
     8701 cagggtgggc gtcaccggcg acatcgccgg acggcttcac cgttcgcgcc gacacgtcgc
     8761 ggtgcgccgg ttgcgtggcg gtggcacgac gagcgcgcac ggcttcgacg ggggccgggc
     8821 gtgacgcctt cccgttcgag gcgacgagct tcacgcgctg ctcgtccgct gcgtgcgcag
     8881 gttgcgcctt gcgcaggttg cgcatctgct cggtggtcag cggctggccg tgctggcgcg
     8941 cgatcagctt ttcgcgcgcg gcgaagccga cgggacgcgg cggcggcgcg tgccgcgcga
     9001 ccaccggccg tgcgaaggcc tcgcgctcgt gggccacggc ggggcgcgca gccggccggt
     9061 gcaggccaat gctggccttg cccggattga cgtccggccg caccgacagc tgcgcgttgc
     9121 ggagctgctt cggatccagc ttcagcgccg ccgggtgcac cggccgcgca ttggcgaaga
     9181 tcgtgcgtgg caccatcgtg accgcgcccg gcagctcgcg atagcgatag tggttgcgcc
     9241 ggtggatgtc ggcgtaccgc tggtggcggt agtcgtcgta gaaattgttg atcaccaccc
     9301 ggttgacgta cacgttgcgg atgttggtga cgttgacgtt ggtgaagtag ccgcgcgagg
     9361 catggaacca tggcacgtac acgtcgcgcg gacccagcgg gaaccatccg accggaccgc
     9421 cgccgccaag gcggatcgac agcgacaccc ccgatccgcc cacgaaggcc accagcgccg
     9481 gcgcgtacac cgggcgcacg ttcacgggcc ccggcatcca gccccagcgg ttctggacgt
     9541 acacccaacg gccgtaatgg aatggcgcga atccccacgg ctggttgtcg acccaggtcc
     9601 agccccaggg atcgatccat gcccagtggc cgtagcgata gggcgcccag ccgaccggca
     9661 ccgacctcgg gtaccagacg ttgccgtact cgggcaccgc gttccactgg ccgtaccggt
     9721 acaggtcgtc gccgccgatc atgtcggacg ggatgtattg ccgctgctgc tcggcgtagt
     9781 gcgtgtagtt ggtgtcgcgg gtttcgcaga agcggtcgaa gtcgtcgggt tcgggcaggc
     9841 cattggcctc gacgctcgcc agcgtcgaat cgtcgaagcg ataggaggtg cctgcctgga
     9901 cctcgcggct ggcgccgttt tcgccgtaga caacgcccga gccttggaac acggtgacca
     9961 tgctgccggt gccggtggga ctgttgtcga tccggtagat cccgggggcg gtggcgacga
    10021 aggcgaccgt gggcgtgtcg acctcgaagt tttcgttgtc gcccaggtcg cgcaccgcga
    10081 ggttcagggt gccctgggag agttcgatct ggacgttgcc ttcgtcgaga ttgaggaagt
    10141 cgaacgcgct gccgtggtcg atccgcaccg aggcgtcgcc gagttccagc accgcgcggc
    10201 cgtcatcgcc ggtcagcagc cggtcgccga ttaccatcgg ccggttgacc tcgaccgaac
    10261 cccagtcgtc ctcgccggcg ggcgcgaact gcacctggcc ggacagccag gccagccgcg
    10321 cgacacgccc gggcgcctgc ccggcatcct gatccatcac ggggccgttc aaggggttgt
    10381 attgctgcgc cggcgccgtg gcggcgaacg tgagcagcgc gaggcacgtg cagcccgtga
    10441 ccagcagcgg gcggagaagg cagcgtaggg acatggcgac ccccgaagta tccgggcacg
    10501 tggaggtgga tcgccggacg gcgcccttcg cccgacgttg ctagttaacg ccatgcaagc
    10561 tgaatgtaat attacactgg tcagtccagt atctcggccg cgcccccgag gcctgcggcg
    10621 ccgttgcgca tgcgcggcgg gcctcgctac cattcccctc cctgcgcggg tgtagctcaa
    10681 tggcagagct gtagcttccc aagctactga cgagggttcg attcccttca cccgcttgga
    10741 tccctggcat ggtcgatacg cgtggtgccc ttcccgtcct cggctggcgc gaacgcgtcg
    10801 cgctgccgcg gctggggatc ggccggatca aggccaagct cgacaccggt gcccgttcgt
    10861 ccgcgctgca cgtggattcc ctcgaatcgt ttcgccggga cggcgatgac tggctgcgct
    10921 tcgacctcgc caccggcagg cgtcctccgc gccgggtggt ttgcgaggcg cccgcgctgg
    10981 atcggcgccg cgtgaccgac tcgagcggca atgccagcgt gcgctggttc atcgaaaccg
    11041 aggtgctgct gggtaccctg gcgctttgca tcgaagtcag cctcaccgat cgccgcggca
    11101 tgctgttccc gatgctgctc gggcgaacgg cgctcgcggg acgcgcgctc gtcgatcccg
    11161 cgcgctcgta tacgctttcc ctttcgaccg ccacgccgcc accgacccga tgaaactcgc
    11221 gatcctgtcg cgcaacgcac gcctgtactc gaccatgcgc ttgctggcgg cggcgcgcgc
    11281 ccgcgggcac aaggcgcgcg tgctcgaccc gttgcgctgc tacatgcgca tttcgcacga
    11341 tggcttcggc gttcattacg gcggccggca actgaagggc tacgactgcg tgatcccgcg
    11401 catcggcgcg tcggtgacct tctacggcac cgcggtgctt cgccagttcg agatgatggg
    11461 ggtgtacacg ccgagtccct ccgaagcggt gttgcgcgcg cgtgacaagc tgcgcagcct
    11521 gcagatgctc gccagccacg gcatcgacct gccggcgacg gtgttcggcg acaaccccga
    11581 cgacaccggt gacctcctgg cgatgctcgg gccaccgccg cacgtgatca agctcaacga
    11641 gggcgcgcag ggcacgggcg tggtgctggc cgagaagctg cctgcctcgc aaagcgtgat
    11701 cgaggctttc cggggcctgt acgccaattt cctggtccag gaattcgtgc gtgaagcgaa
    11761 cgggcgcgac atccgctgct tcgtcgtggg cggcaaggtg gtggcggcga tggagcgcgc
    11821 cgcggtggaa ggggacttcc gcgccaatct ccaccgtggc ggcaccggca gtcccgtgaa
    11881 actggagtcg gaagagcgcc gcacggcctt gcgcgccgcc aaggttctcg ggctcggcat
    11941 cgccggcgtc gacttgctgc gttccgaacg cggcccgctg ttgctggagg tcaatgcgtc
    12001 accggggctc gaagggatcg agaaggtcac cggcgtggac gtggccgacg cggtgatccg
    12061 gcatctcgag gccgcgctcg agtgagcctg taatcaacca aggttatcgt acgaaacgca
    12121 tcgttgaaat gcgcatggca tgggaatgga ctggcatcga ggcgcccgcg gggcgcggcg
    12181 atgatcgggg agggccatgc gaaattccaa tcggcgggct tcaggcctcc tggcggccgc
    12241 gctcgcgttg gccggctgtt ccgggagtgc gccggtgcgg tacgcgaaag gcgcggcgcc
    12301 gacggcggac tccggcatcg ggcaggtcca tgtcggcgcc ggcaccaagg cagacctcga
    12361 cgcgaagatc gcagccagat gctgccgggc ggacgctggc aggacgtcag cgacagcgag
    12421 cgcgcggagc actcggcgag cttcgcgcgg atggagaaac cgttcgacga gcatgaagtc
    12481 gtcgacgaca tgggccaaca tgcgcgcctg accctgctgg atgcgcagaa cgacgtcaac
    12541 gtgatcctgc ccacgacgac ggccatcgct tgatctgcat ggtccaggca accaccggct
    12601 cgcaccttgc gctcaagaca tgccagacct gcgcacagtt gcagaagtgg gcgcgcgaaa
    12661 atcaggcgga cgtgcaaaag cgactggaaa cccagcatct tccgcgtcct ccttgcgggc
    12721 tcgccaaagg ttagcctgcg tgctgagtgc gggcgtgaga ggcgccgagc ccggccccgc
    12781 caccggccgt tggcgccccg tggggagcgc ggcaagcaag ccggaccggc cgcgaagttc
    12841 tagtacgaat ccgatcgttg acaggcttcg gcggttgtgc gtagcatggc caatccgcgt
    12901 cgtttccatt cggtacgcgg caactgctgg ggagtttcga cgatgaagac ttcgagtggc
    12961 ctcattctcg cggccgcgat cggtgcactg atgctgcagg gttgtgccgc cacgggagag
    13021 accagggcca gcgccgcccc gacggctccg cccgccagcc aggcggctcc gatggccgcg
    13081 gcgccggcgg cgcaggttcc ggaggaagac ggcaagccgg tcgtcaaggc cgacaccgcg
    13141 gaaaatttcg ccgcggtcgt cgcggcgatc caccagcaga tgcaaccggg cggccgctgg
    13201 cagtacgtcg acagccggga acgcgccacc atcaacggca gcttcggcga catgcagtcg
    13261 ttgtatgacc agttcggcag cgtggacaag atggaccagg ctgccaggat gcgattgctc
    13321 gccgaccaga acacagtgaa tgccatcctc acccaaaagg acggtgaccg cctggtctgt
    13381 gaaaacagga tgccggtcgg ttcgcacttg ccggtcaaga cctgcaagac ctatgcccag
    13441 atgcaggccg aacaacgcgg cgcccaggaa ttcatgcggc agcgcgaggg cacgatgcaa
    13501 caaaggcatt gacatgccat cgctcgggat tcgctcgcca gctgcagtca ttcgatttcc
    13561 ggtcgctgcg gttccgaact ggcatgtggg tctttgcgag gactgagccc ctgccggcag
    13621 ctcggcaggg ccggttgggc gcagcggcgg gcggagctgg tgcgcaggtg gatcgccgga
    13681 gccggacatg atggtcgccc gcgaacgaaa attgccccgg cccaccacgg ccgaactcga
    13741 aatcctgcag gtgctgtggg agcacggtgc ctgcacggtg cgcgaagtgc acgaggaatt
    13801 gcatcggcgc gaccgcaccg gttacaccac cgcgctcaag caactccaga tcatgttcga
    13861 caaggggctg gtgctgcgca acgagtcccg gcgggcgcac gtgtaccgcg ccgccgtgag
    13921 caaggaacgc acccagaaga aattcctgtc cgacatgctg caacgcgtgt tcgacggttc
    13981 gccgtcgcgc ctggtgttgc aggctcttgg tgaccacaag accagccgcg cagaactcaa
    14041 cgagatccgc gcgctgttga accgcctcga caaggaagcc gaaccatgaa cgtcgccggg
    14101 atcttctcga tcggagtcgt ggccctgggt tggagcctgc tgcacttcgt gtggcaggcg
    14161 accctcgtgg gcatcgtgta cgcggtgacg cgcggcatgc tgccacgcgg caatccgcgt
    14221 tacctcgccg cgatgctggc aatgatggcg ctggcgattt ggccgctggt caccgcgtgg
    14281 cacgaggcgg cctcgtacat gggcacggtg ggtcttccgg gcgccgtggt gtccgcagct
    14341 ccaacccgcg cttctgcatc cgccgcggag tcccccggct ggcaggcgtg gttcgacgcg
    14401 atgatgccgt ggttggtgct ggcatgggcg gccggcgtgg gggtcctcgg gacgagggtt
    14461 taccggcaat gccgcggctt gcgttcgatc ctgcgcgcgg cgggggtcca gcccgagtgg
    14521 caggcgcgtg cgcgagccat cgccgaccgc atgcgcctgc accgggtggt gccggtgctg
    14581 gtcagcctgc gggttggcac cccgaccttg atcggatggc tgcgaccagc cgtggtattg
    14641 ccggtcgcgg tgctggcgcg gatgccggcg gcgcaggtcg aaatggtgct cgcccacgaa
    14701 ctggcgcacc tcaagcgcct cgatcccttg gcgaacctgt tccaggtggt gctggagacg
    14761 ctgttcttct accacccggt ggtccactgg atttcccgcg acgcgcggca cgagcgtgag
    14821 ctgtgctgcg atgcgctggc gctgcgggcg actggtggcc agcgtcgcga cttcgtggcg
    14881 gcgctcgccg tcctcgcgga atttcgcagc agccacgccg acctggcgct ggccgccagc
    14941 ggcggtgcct tggccgagcg ggcctggttc attgccggcg acgcgccgga gccccggcac
    15001 cgcactggtg gcctagcgtt cgccggcctt gcgctgctcg ggttggcgat ggtattcggg
    15061 gtagcctggc ggcaggaaat ccggagccag cgcgtcgaag acatgctggc cggcggggtg
    15121 tccgcgctgc gacagagtct cggcggcatc gccggctcgc cgttgccgct cggctaccaa
    15181 ctccccgacc agcggccgcg gctggcgccc gtgtcgctcg ccgcgctcgt tgcgccgacg
    15241 ccgacgctgg caccgattgc cgttccgagc gcgaagaagg ccggcgtggc gtcaccgcgg
    15301 gttgcggtgc aggacctcgc gccgccaccg gcaggcgtgc atccggtcgc ggtggcttcg
    15361 ccgttgcccg caagcgcggg tgggtcgtcc gactcgaccg tgcagccgcg cgcgatccgc
    15421 gcgccgcagc ctgagtatcc ggcgcaggcg atgtacagcg gcgtgcaggg ccgggtcgag
    15481 atccagttca ccttgagcgc ggccggtgtt ccgcgcggaa tgcgggtggt gggcacgtcg
    15541 ggatcgacgt cgctcgatgg cgccgcgctc gacgcgctcg cacgctggcg cttcacgccg
    15601 cccggggttg cgggacggca gtaccgccaa accttcaatt tcgagctgga gcccggcgcg
    15661 gctgcggccg gcatggccgg gagccagggc tgcgtgcgat cgaccggcac ccacatctgc
    15721 aggcagccga tcgatgaggc cgcggccgtg cggacaccgg gtccgggccg ctgaagggac
    15781 gatcatcggc gcagcgcgcg ggtgcgattc tcgctaagct tgcagcctgc gcgaggaagc
    15841 gcggcagacg ggactatcca tgcgtgtgct ggtgatcgag gacaacaatg acatcgccac
    15901 caacatcggc gattacctgg aagaccgggg ccacgtggtc gacttcgccg gcgacggcgt
    15961 gaccgggctg cacctcgcgg tggtcaacga cttcgacgtg atcgttctgg acttgacctt
    16021 gcccggcatg gacggcatcg aggtctgcaa gaagctgcgt ggcgaggccc acaaacacac
    16081 gccggtgttg atgctgaccg cgcgcgacgc gctggaacag aagctcaccg gtttcgaatc
    16141 gggcgccgac gactacatga ccaagccatt cgcgctgcag gagctggccg cgcggctgga
    16201 ggtgctcgcg cgtcgcggca agggacccac cagccgggtg ctcaagatcg ccgacctcac
    16261 ctacaacctc gacaccctgg tggtcacccg cgccggccac tcgatccagc tgaacccgat
    16321 cggcctgaag ttgctgcagg cgttgatgga ggcgagcccc tcggtggtca cccgccagga
    16381 cctcgaacag cgcgtttggg gggaagagct gccggactcc gacagcttgc gcgtgcacat
    16441 ccacggcctg cgcgcggcga tcgacaagcc gtacggcaag ccgctgatcc acacgcgcca
    16501 cggcatcggc taccgcatgg tcgatccgga tgcagtcccg gcgtaagctt cgcgtccggc
    16561 tgatcgtcac cttcacgctg ttcggcttct gcctcagcgc gctgtttgcc gtggcgtcgc
    16621 tgtacgtgcg cgccaaggtc gagaaccagt tgatcgacca gagcctggag gccgatctcc
    16681 ggcaggcggt ggagaacaag cacctgcatc cggaccagcc gctgcagtcg aagctgatcg
    16741 tggtgtggct ctggaacgac cgcaccgtgt accaggcgcc actggcgtgg cagggcctgc
    16801 cgcccggcgt atacgacatg cgcggcggcg acaatggcct gccgcagcac tacaagctgg
    16861 ccgtggtgcg caagtacggg atcgatgcat tcctcaccta cgacatcagc cgcgaaggac
    16921 tcggcaagcg gcaattgctg accagcctgg tcggtgccgt cgtcctgttc acgctgctgt
    16981 cgctcgcggt gggttactgg ctgtcgcggc gggtgatgcg gccggtgacc gagctcgcgc
    17041 aacgcctgcg cgcgttccgc agtggccggc agctcgaatc gctggcgccg catttcgccg
    17101 aggacgaagt cggcgagctg gccgctgcgc tcgacgacta cgcccagcgc cagagtgcgc
    17161 tgatcgagcg cgaccgcgaa ttcaactccg atgtcagcca cgagctgcgc acgccgctcg
    17221 ccgtgatcgc gagcaccacc gagctgctgg tcgcgacccc cgaactcccc gagaaactgc
    17281 gcacgcggct gcagcggatc gagcgcgcgg tgcggcaggc caccgagctc acccaggcgc
    17341 tgctgctgct gtcgcgcgcg gaacgttccg gcccggtcga tggcgagacc accgacgtcg
    17401 ccaaggtcgt gctcgaggtg gtggacaccc accggccgga cctcggccgc aagccgatcg
    17461 agcttcgtac ggagatcgag gcgccgctga cggtcgaggc accgtcgtcg gtggtggcag
    17521 tcgcgctggg gaacctcgtg ggcaacgcct gcaagtacac ccaggaaggc gagatcgtgg
    17581 tgcgggtgca gcggggcagg gtgcgggtcg aagacaccgg ccccggcatc aagcgcgagg
    17641 acgccgagcg gctgttcgag cgcggggtgc gcggcgaggg cgagacggtc aagggcgcgg
    17701 gcctcggcct ggccattgtg cgccggctgt gcgagctcta cggatggcag gtggcgctgg
    17761 cgccgcgcgc tgagcggggc gccgtggcga ccctggactt cggggatgtg ggcgaggcct
    17821 gatccttgcg gtgaaaccgt cgatcccgac gcggtttcgc cccacggcgc cggcgtatac
    17881 tcggccggcc gcgcccgatc gtccgcttgg ggaacgttgg ctcgcagggc acgtcggcgc
    17941 cgtgcacagt gcccgttgcc gggattggcg cggaaattgc acgaaatttc agttcgattg
    18001 caaagatttg caacatgacg ggcaacatac tcgggagggg gtagatggat atcgccgaac
    18061 tgctggcctt ttcggtcaag aacaaggcct cggacctgca cttgtcggcg ggcctgccgc
    18121 cgatgatccg cgtggacggc gacatccgcc gcatcaacct gccggcgc
//
LOCUS       DASSNH010000305         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1669602, whole genome shotgun sequence.
ACCESSION   DASSNH010000305 DASSNH010000000
VERSION     DASSNH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669602"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VFK00_11450"
     CDS             complement(<1..1462)
                     /locus_tag="VFK00_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7593602.1"
                     /translation="MDVSYLLDSLNAAQREAVAAPPGHYLVLAGAGSGKTRVLTRRIG
                     WLVEAEAEPPWSILAVTFTNKAAGEMRGRLGALLPGGTRGLTVGTFHGIAHRLLRQHW
                     REAGLPETFQILDADDQQRLVKRVVAGLGIDEARYPPRQACWQINAWKDEGKRPEGIE
                     PGDHPVARTYLKVYEAYEQACRRAGLVDFAELLLRAHETFRDNDALRTHYQQRWRHLL
                     IDEFQDTNALQYAWIRLLAGKTGKVFAVGDDDQSIYGWRGAKVENMRTFLRDFPDAKT
                     LRLEQNYRSTATILEAANAVIAQNPSRLGKQLWTDTGKGDSIALYAAYNEQDEARFVI
                     ERIREHIEGGGEASDIAVLYRSNAQSRNFEEQLHQHDIPYRVYGGQRYFERAEVKDAL
                     AYLRLAANRGDDASFERALATPPHGVGERSLEVLRRRARADDSSLWDAALAELTGGGL
                     NSRAKNALRGFMMLVDGLARTTAGTGNRAQGTEARAP"
     gene            complement(1542..1763)
                     /locus_tag="VFK00_11455"
     CDS             complement(1542..1763)
                     /locus_tag="VFK00_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593603.1"
                     /translation="MRAGLIIVGIVLLGLGVWVAIGNLHYNDTDTKAQLGPVKVEATS
                     QKPVPAPIGYAGIVVGGILIAAGALKKSR"
     gene            complement(2317..>2565)
                     /locus_tag="VFK00_11460"
     CDS             complement(2317..>2565)
                     /locus_tag="VFK00_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET7593604.1"
                     /translation="PMQGTAADIIKRAMVAIDGWLNSRDDAAHMLMQVHDELVFEVRA
                     DAVDAVRDGVRERMQGAAELVVPLEVDIGVGFNWGEAH"
BASE COUNT          407 a          850 c          840 g          468 t
ORIGIN      
        1 cgggcgcgcg agcttcggtt ccctgtgccc tgtttcctgt tcccgctgtc gttcgagcca
       61 acccatccac cagcatcatg aacccccgca gcgcattctt cgcccggctg ttcaatccgc
      121 cgccggtgag ttccgcgagc gccgcgtccc acagcgagct gtcgtcggcg cgcgcccgcc
      181 gacgcaagac ttcgaggctg cgctcgccca ccccgtgcgg cggtgtcgcc agtgcccgct
      241 cgaacgaagc gtcgtcaccg cggttcgcgg cgagccgtag gtaggccagc gcatccttga
      301 cctcggcgcg ttcgaaatag cgctggccgc cgtaaacccg gtacgggatg tcgtgctggt
      361 gcagctgttc ctcgaaattg cgtgactgcg cgttcgagcg gtacagcacg gcgatatcgg
      421 aagcctcgcc gccgccttcg atatgttcgc ggatgcgctc gatcacgaag cgcgcctcat
      481 cctgttcgtt gtaggccgcg tacagcgcga tggaatcgcc cttgccggtg tcggtccaca
      541 attgcttgcc cagccgcgac gggttctgcg cgatcaccgc attcgcggcc tcgaggatgg
      601 tcgcggtgga gcggtagttc tgttcgaggc gcagggtctt cgcgtcggga aaatcgcgca
      661 ggaacgtgcg catgttctcg accttcgcgc cgcgccagcc gtagatcgac tggtcgtcgt
      721 cgccgacggc gaagaccttg ccggtcttgc ccgcaagcag gcggatccac gcgtactgca
      781 gcgcgttggt gtcctggaat tcgtcgatca ggagatgccg ccagcgctgc tggtagtggg
      841 tgcgcagcgc gtcgttgtcc cggaaggttt cgtgggcgcg cagcagcagc tcggcgaaat
      901 cgaccagtcc ggcgcggcgg catgcctgtt cgtaggcctc gtagactttc aggtaagtgc
      961 gtgcgaccgg atggtctccg ggttcgatgc cctcgggacg cttgccttca tctttccagg
     1021 cgttgatttg ccagcacgct tggcgcggcg gatagcgtgc ctcgtcgatt ccgagtcccg
     1081 cgaccacccg cttgaccagg cgctgctggt cgtcggcgtc caggatctgg aaggtttccg
     1141 gcaggccggc ctcgcgccaa tgctggcgaa gcaggcggtg cgcgatgccg tggaaggtgc
     1201 cgaccgtgag cccgcgggtg ccgccgggca gcagggcgcc gagccggccg cgcatctcgc
     1261 cggcggcctt gttggtgaag gtgaccgcga ggatcgacca aggcggctcg gcttcggctt
     1321 cgaccagcca gccgatgcgg cgggtcagga cccgggtctt gcccgaaccc gcgcccgcca
     1381 ggacgagata gtgcccgggc ggggcggcga cggcttcacg ttgcgcggcg ttgagcgaat
     1441 ccagcaggta cgaaacatcc atcgccgcaa ttgtagccgc gcgatgcgcg tggccttgcc
     1501 gccgcgccgg gcggtgatgt cagcccgcag ggagcgaacc ctcagcgcga tttcttcagc
     1561 gcccccgccg cgatcaggat cccgccgacg acgatgccgg cgtagccgat cggcgcaggc
     1621 accggcttct gcgaggtagc ctcgaccttt accggtccca gctgcgcctt ggtgtcggtg
     1681 tcgttgtagt gcaggttccc aatggctacc cacacgccca acccaagcag cacgattccc
     1741 acgatgatca gtccggcacg catggcagct ccctttgcgg attgaaggac gcccgatcct
     1801 acacgaactc ccgcatggtc cacgccgacg gacggcgccg gtacatcgcg cgtacagcga
     1861 ggaagctgca cgcgcgtctg aaaataaaat gccccgagag ccagggggga gctctcgggg
     1921 ccgggcgaag ccgaacctgg ggagggattc gacctcgggg gttcgctcag ggaggtgagt
     1981 gaaccgggac gccgttgcgt gcgtccgagg cattagatgg ggtcccccgc cggaagttca
     2041 aaatcttttt ttcgtaggat tcataatgcg ttcattcgcg gggcgcccca tgacttccgg
     2101 catgtttttg cgttcatcca tgaacgctgt ttcgtcggat cttccatcgc cgcaacgatg
     2161 ccaaaccaca agcgccagaa cggccatccc tggcctgact tttcacaaaa gccgctccgg
     2221 gcggggttca cacggcggtg tccattttat ccagttgggg tttggccatc caaacgaagc
     2281 cgccgcccga acggcggccc ggcgttcaca aggcaactaa tgcgcctcgc cccagttgaa
     2341 cccgacgccg atatcgactt ccagtggcac cacgagttcg gcggcgccct gcatgcgctc
     2401 gcgaacgccg tcgcggacgg catccacggc atcggcgcgg acctcgaaca ccaactcgtc
     2461 gtggacctgc atcagcatgt gcgcggcgtc gtcacgagag tttaaccagc cgtcgatcgc
     2521 gaccatcgcg cgcttgatga tgtcggcggc ggtgccctgc atcgg
//
LOCUS       DASSNH010000306         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_549048, whole genome shotgun sequence.
ACCESSION   DASSNH010000306 DASSNH010000000
VERSION     DASSNH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549048"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="VFK00_11465"
     CDS             complement(<1..1973)
                     /locus_tag="VFK00_11465"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET7593605.1"
                     /translation="MSRIRLLPIRLLPAALAAALVMLGSTTIASAQYYDQGPSYDQSA
                     PQDQNAQADPSDRVARLAYLTGDVEFAPAGENDWGSADVNRPLTTGDRLMTGDDGRAA
                     LELGDAALRINDNSAFNFLDLDDATTQVELSQGTLNLRVRRMGDGQVYEVDTPTVAFV
                     ASEPGTYRVDVAPSGNGAMVTVFEGSGTVYGENGASRPVQAGQSYRFDDSTLANVAAQ
                     GVPAPDDFDRFCQSRDQRYTNSVSRRYVSDDVVGYDDLDQYGDWQDTSDYGQVWYPSH
                     VAADWAPYRDGHWAWIEPWGWTWVDNEPWGYAPFHYGRWAYVRNRWGWVPGPRTVRPV
                     YAPALVAFVGGSGFSVSVSVGGGGPVGWFPLGPRDVYVPPYRASRHYFTSINVTNIRN
                     VYVNKTVINNYYGSYAAGRPYSGARYVYRDNPRAFTAVPRNVFAGARPVRSAVLHVNR
                     AALAKATIAPIPHVTPTRASLAITPPKRPIAKPVAKAFDRTVVARHAPPPRAVPFAAR
                     EKVIARQGGAPIPVTQLRQMRQQHAKAQPQPERVKVVAAAPNRPGQPQARTPDGRQPV
                     QAPRSGKRPPARASIHPIQVPVNPPKGGAANPPREQPLRPGELPSARFAHGPQNGNPP
                     GRAAENQQRAQQVRQQAEQARQNAGQDRQQAEQA"
     gene            2165..2238
                     /locus_tag="VFK00_11470"
     tRNA            2165..2238
                     /locus_tag="VFK00_11470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2197..2199,aa:Gly,seq:ccc)
     gene            2395..>2736
                     /locus_tag="VFK00_11475"
     CDS             2395..>2736
                     /locus_tag="VFK00_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016945000.1"
                     /note="responsible for the addition of glutamate residues
                     to the C-terminus of ribosomal protein S6; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HET7593606.1"
                     /translation="MKIAILSRNTRLYSTSRLVAAGRERGHRVRVLDPLRCYMRIAHD
                     GFALHYGGKELKDFDCVIPRIGASISFYGTAVLRQFEMMGVYTPNSSEAVLRARDKLR
                     SLQMLAKRGLDL"
BASE COUNT          418 a          919 c          937 g          462 t
ORIGIN      
        1 gcctgctcgg cctgctgccg gtcctgtccg gcgttctgcc gggcttgttc tgcctgctgg
       61 cgaacctgct gcgcgcgctg ctggttttcc gcggcacgtc ccggagggtt gccgttctgc
      121 gggccatgcg caaagcgcgc agatggcagt tcgcccggac gcaacggctg ctcgcgtggc
      181 ggattcgccg cgcccccctt cgggggattc accggcacct ggatcgggtg gatggaggcg
      241 cgggccggcg gacgtttacc gctgcgtggc gcctgcacgg gctgccggcc atcgggcgtt
      301 ctcgcttgcg gctggccggg ccggttcggc gcggcggcca cgactttcac gcgttccggc
      361 tgcggctgtg ccttggcatg ctgctgccgc atctggcgca attgcgtcac cggaatcggc
      421 gcgccgccct gcctggcaat taccttctcg cgcgcggcga acggcaccgc ccgcggcggc
      481 ggcgcatgcc gcgccaccac ggtgcgatcg aacgccttgg ccaccggctt cgcgatcggt
      541 cgcttcggcg gcgtgatcgc gaggcttgca cgggtcggcg tcacgtgcgg gatcggcgcg
      601 atggtcgcct tcgccagggc ggcacggttc acgtgcagca ccgccgaacg gaccggcctt
      661 gcgccagcga agacgttgcg gggtaccgcg gtgaaagcgc gcgggttgtc acggtagacg
      721 tagcgcgccc cgctgtacgg ccggccggcc gcgtagctgc cgtaatagtt gttgatcacc
      781 gtcttgttga cgtacacgtt gcggatgttg gtcacgttga tgctggtgaa atagtggcgg
      841 ctggcgcgat aaggcggcac gtacacgtcg cgcggcccga gcgggaacca gcccaccggt
      901 ccgccgccgc cgacgctgac ggaaaccgag aagcccgaac cgccgacgaa cgcgaccagc
      961 gccggcgcat acaccggacg caccgtgcgc ggcccgggca cccagcccca gcgattgcgg
     1021 acatacgccc agcggccgta gtggaacggc gcatagcccc agggttcgtt gtccacccag
     1081 gtccagcccc agggttcgat ccacgcccaa tgaccgtcgc ggtagggggc ccaatcggct
     1141 gcgacgtgac tggggtacca cacctggccg tagtcggagg tgtcctgcca gtcgccgtat
     1201 tgatcgaggt cgtcgtaacc caccacgtca tcggaaacgt agcggcgcga caccgaattc
     1261 gtgtaacgct ggtcgcgcga ctggcagaag cgatcgaaat catccggcgc gggcacgccc
     1321 tgcgccgcca cgttggcgag cgtgctgtcg tcgaagcgat aggactgccc ggcttgtacc
     1381 gggcggctgg caccattctc gccatacacg gtgccggaac cttcgaacac ggtgaccatc
     1441 gcgccattgc cgctgggcgc cacgtccacg cggtaggtgc cgggctcgct cgcaacgaag
     1501 gccacggtcg gcgtgtccac ctcgtagacc tggccgtcgc ccatccggcg cacgcgcagg
     1561 ttcaaggtgc cctgcgacaa ctccacctgc gtggtggcgt cgtcgaggtc gaggaagttg
     1621 aaggcgctgt tgtcgttgat gcgcagcgcg gcatcgccca gttcgagcgc cgcgcggccg
     1681 tcatcgccgg tcatcaggcg atcgccggtg gtcaacgggc ggttgacgtc ggcactgccc
     1741 caatcgttct cgccggcggg cgcgaactcg acgtcgccgg tgaggtaggc cagccgcgcc
     1801 acgcgatcgg atggatcggc ctgggcgttc tggtcctgcg gggcgctctg gtcatacgac
     1861 ggcccctggt cgtagtactg cgcgctggcg atggtggtgg aaccgagcat caccagcgcc
     1921 gcggccagtg cggccggaag caatctgatc ggaaggagac gaatgcggga catggcggac
     1981 tccgcgactg cgctgcttga gcggcaagca acccatgcgc acctggtcca ggcggcacgc
     2041 ctacatcaca gcccagacgg gctgaatgaa cgccatccgg aacctgcccc ctaagcggcg
     2101 gcaaagaatc cggtcagccg ccgttgcgcg agtgcggcgc ggctccctac aatcgcgatt
     2161 cgatgcgggt gtagctcaat ggtagagctg tagcttccca agctactgac gagggttcga
     2221 ttcccttcac ccgctccatg gcacgttccg gcaacttggc gcgtgaggag tcatcaccga
     2281 ttgcccagcg tggggctccc gcacctccac aacccccatc cgttcgcgcg cacttgcgcg
     2341 aacggattgc ccccttaact taagggggct gcaaagcccg cactccgaga tgccatgaaa
     2401 atcgcgatcc tctcgcgcaa cacgcggctg tattcgactt cgcggctggt ggcggcgggg
     2461 cgcgagcgcg gccaccgcgt gcgcgtgctg gatcccttgc gctgctacat gcgtatcgcg
     2521 cacgacgggt ttgcgttgca ctacggcggc aaggagttga aggatttcga ttgcgtgatc
     2581 ccgcgcatcg gcgcctcgat cagtttctac ggtaccgcgg tgctgcggca gttcgaaatg
     2641 atgggcgtgt acacgcccaa ttcgtcggaa gccgtgttgc gcgcgcgcga caagctgcgc
     2701 agcctgcaga tgctcgccaa gcgcggactc gacctg
//
LOCUS       DASSNH010000307        10216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_660811, whole genome shotgun sequence.
ACCESSION   DASSNH010000307 DASSNH010000000
VERSION     DASSNH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660811"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFK00_11480"
     CDS             <1..268
                     /locus_tag="VFK00_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7593607.1"
                     /translation="ALSPIGYALVGKLAAPDEASLAMGGWFFGVGISYDISGQIATLT
                     TAHGTTGIAGYEHVFGWLLVGGVAIALVYLIAAPWIVKLMHGVR"
     gene            291..1805
                     /locus_tag="VFK00_11485"
     CDS             291..1805
                     /locus_tag="VFK00_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET7593608.1"
                     /translation="MIEAIDNTALPETPDFFPQRFGHPKPLWMLFMTEFWERFAFYSV
                     SWALALYIVQQFYHGDPAGQAWASGIFGAYTALIYGTCIFGGWVADRVIGYQRTILLG
                     ALVMAAGLFVLMLPSRTMLLLGLALVIVGDGLFKPNISTMVGQLYGRDDPRRDRGFTL
                     FYMGINGGALLAPLLTGWMAAHFTATPMQQNFRLVFAAAGIGMLISLLWFWFGRRQLK
                     HVGRPAAGSEGPARVAWVVLGVLVSVPLVFLLLSRLGATGLQWLLGLLFVVVAAMLLV
                     EAARHGRVQVHRVVAMLIIFAFNVLFWMFYYQLGTSFNFLAENIVDRVMPGGWIFPVG
                     WFQSVSPLAIIVFAPLVTLTWAWLDRRRMEPSIPRKFGLGLVFNSLGFLVLMFALSKL
                     LGANGLIPFWPLVLCYVMQTLGELCLSPIGLSMVTKLAPPRLVGFAMGGWFLSLSVGG
                     NLSGLFASRIAGTGGMTAGSALSGFTFGFWLLLGAGVLLLLVSPLVNRLMHGVR"
     gene            complement(1777..2247)
                     /locus_tag="VFK00_11490"
     CDS             complement(1777..2247)
                     /locus_tag="VFK00_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7593609.1"
                     /translation="MPRHAPIELEKFVPYQLSIVSNTVSQAIAEEYQQRFDLGTTEWR
                     VMAVLARYDGEGLSARAVARLTAMDKVAISRALARLVMKGRVLRRTHHGDKRRSVLRL
                     SAAGWRIHDAVAPLARRHERELLERLEDEERRSLTRILDKLMAGEANAPRASAG"
     gene            2434..3522
                     /gene="hppD"
                     /locus_tag="VFK00_11495"
     CDS             2434..3522
                     /gene="hppD"
                     /locus_tag="VFK00_11495"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET7593610.1"
                     /translation="MNAQPNAGMQPTTFENPMGVDGFEFVEFAGPDPKSLHDLFKRLG
                     FTAVARHKSKAVTLYRQGACNFLLNEQPDSFASDFVQSHGPCACGFAIRFSRPASEVL
                     EATLANGAKEITHRAHTRAVDAPVIEGIGGCMLYLVDRYGGKGSVFDDYEWLVPEGQR
                     QPVGFGLTFIDHLTHNLHFGEMAKWAGYYERLFNFREIKYFDIKGAKTGLVSKAMTAP
                     DGMVRIPLNESNDPKSQINEYLDEYKGEGIQHIALFTDTIYDTVEKMRAAGVEFLDTP
                     DAYFEVIDVRVPNHGEDVPRLAKNKILIDADMETKRKLLLQIFTQNCIGPIFFEIIQR
                     KGNEGFGEGNFQALFESIERDQMKRGVL"
     gene            3735..5048
                     /gene="hmgA"
                     /locus_tag="VFK00_11500"
     CDS             3735..5048
                     /gene="hmgA"
                     /locus_tag="VFK00_11500"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET7593611.1"
                     /translation="MSDSPAYLTGFGNEHASEALAGALPVGQNSPQKVPYGLYAEQMS
                     GTAFTAPRHANRRSWLYRIRPAAVHGAFEAYSQPRFHNRFDEAPTSPDQMRWSPFSLP
                     DAPTDFVDGLFAMAGNGAPAMQAGVGIHVFACNKPMQGRFFYDADGELVVVPQQGRLR
                     ISTELGVLEVEPQQIALVPRGLRFRVDPMEGTARGYACENFGAPLRLPELGPIGSNGL
                     ANACDFEAPVAAYEDVEGDFELIAKFQGNLWRAPIDHSPLDVVAWHGTHVPYRYDLRR
                     FNTIGSISYDHPDPSIFTVLTSPSDTPGTANMDFAIFPPRWLVAEHTFRPPWFHRNIA
                     SEFMGLIHGEYDAKDASAGGGFVPGGASLHNCMSGHGPDAASYDKALAVDTAKPQHVV
                     DTMAFMFETRNVIHPTRQALESPQRQRDYQQCWQGLEKHFHPSQR"
     gene            5064..6053
                     /locus_tag="VFK00_11505"
     CDS             5064..6053
                     /locus_tag="VFK00_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7593612.1"
                     /translation="MKLATLKEGGRDGTLIVVSRDLGHGVRATGIATTLQQALDDWQL
                     AAPRLHALYDALCLHRHKAVEYAFDLDFNALAAPLPRAYEFVDGSAYLPHVERVRKAR
                     GATVPETFYTDPLMYQATSAGFLGPRDPVRVPSEDYGIDLEAEVVVVTDDVPMAVTPQ
                     QASHHIQLLGLVNDVSLRSLIPAELAKGFGFLQSKPRSALSPVLVTPDELRDAWRDDK
                     VHLPMRTWINGQWFGEAEAGEDMQFNFAQLVAHAARTRPLTAGTIVGSGTIANQDTSR
                     GASCLAEQRTVEKLRDGEARTPFLKYGDTVRIDMHDADGNSIFGSIEQRIEPG"
     gene            6227..7000
                     /locus_tag="VFK00_11510"
     CDS             6227..7000
                     /locus_tag="VFK00_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7593613.1"
                     /translation="MGSRSPQSAGYASSERHTGVPANDGDAERFCSSCAFAEACSVAG
                     YGKTELSELHCLVEHVGPFEPGQRIFRNGDPFRALFAVRSGMVKTTMVDAEGREQVLG
                     FYLPGEMVGLDAIYPEHYPCDGIALETTECCRFSFPAMSALAARQPKVQQHLFRMLSK
                     ELGSATLLAGDHSADERMAAFLVDLGNRYAARGLSGTEFRLSMARADIANYLRLAAET
                     VSRVLGRFREQKLISIDGRRVELLDPARLRELARSVLNT"
     gene            7113..7508
                     /locus_tag="VFK00_11515"
     CDS             7113..7508
                     /locus_tag="VFK00_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HET7593614.1"
                     /translation="MAAWYLQILMIHVGCVIASGTLFFTRGCMMLAGLAAANHIALRR
                     ISVVIDTLLLAAAIALTTIIHQYPFVQAWLTVKVLLLVVYIMLGVFALRRGRTRRTRA
                     ACFGAAMLVFLFIVTVARTHSPLGAFANS"
     gene            complement(7619..7960)
                     /locus_tag="VFK00_11520"
     CDS             complement(7619..7960)
                     /locus_tag="VFK00_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7593615.1"
                     /translation="MDMTSPREALCRLEDIPDGGVLGVSPPDPDGEPLVLARRGSSVQ
                     AWLNICPHAGRRMDYAPGLFLVKDDKLTCAVHGATFALREGGLCVAGPCRGQSLVAVA
                     VQVRDGLVTRA"
     gene            8117..8371
                     /locus_tag="VFK00_11525"
     CDS             8117..8371
                     /locus_tag="VFK00_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593616.1"
                     /translation="MRARAFTLPLPRHPLLRMLVLVGGAILLAGLVAMSLIVGTAVLA
                     VGATWLLVRRWIGRRKRPADPAIIEGEFTVVQRNSLPRTD"
     gene            8430..9302
                     /locus_tag="VFK00_11530"
     CDS             8430..9302
                     /locus_tag="VFK00_11530"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7593617.1"
                     /translation="MNAKPNIDAGRDPVARARDILDHSDPAPTLAELASRVGLSPAWL
                     QRSFRRRFGISPAEFVRARRFGELKRALRQGSDVTDAVYAAGFGSGSRVYEHSDRLLG
                     MPPARYREGGAGIAIRYTTMDSPLGHVLVAATERGLCAVTVGDDDAQLLAALRKEFPS
                     AEVTRVDAGRDEWLAAVVARVARQFNRRTGAAASPALPPLDITATAFQWRVWEALTRI
                     PAGETRSYAEIARAIGEPGAARAVGRACGQNKLALIVPCHRVVRADGTLGGWRWGATR
                     KQALLDRERRAGAR"
     gene            9447..>10216
                     /gene="yedA"
                     /locus_tag="VFK00_11535"
     CDS             9447..>10216
                     /gene="yedA"
                     /locus_tag="VFK00_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HET7593618.1"
                     /translation="MNEVAIPAAAIAAPQRRWLIPLALLSVYVIWGSTYLGIRIGLTG
                     FPPFMLAAIRFFAAGIAMYAWLRLRGVKPPTRRQWRNAAITGVLLLLLGNGMVCFAEQ
                     SVTSGIAAIAIASEPLFIALFLVAYRERPSRGEIVGLAIGFVGVVVLNSGGAMRAAPW
                     AAGALLLATIAWAFGSIWSRHQDMPEGSMNVAAQMLCAAPALALTAWLTGERLPAHPP
                     AASILAIAYLAVFGSIIAFSAYLFLVKHARPTLTASYA"
BASE COUNT         1602 a         3526 c         3328 g         1760 t
ORIGIN      
        1 cgcgctgtca ccgatcggct atgcgctggt cggcaagctc gccgcgcccg acgaggcatc
       61 gctcgcgatg ggcggctggt tcttcggcgt cggcatctcc tacgacatct cgggccagat
      121 cgcgacgctg accaccgcgc acggcaccac cggcatcgcc ggctacgaac acgtgttcgg
      181 gtggctgctg gtcggtggtg tcgcgatcgc gctggtgtac ctgatcgccg cgccatggat
      241 cgtgaagctg atgcacggcg tccgctgaat cacattccga gaagcgacca atgatcgaag
      301 ccatcgacaa tacggccttg cccgagacgc ctgatttttt tccgcaacgg ttcgggcatc
      361 ccaagccgtt gtggatgctg ttcatgacgg agttctggga acgcttcgcg ttctacagcg
      421 tgagctgggc gctggctctg tacatcgtgc agcagttcta tcacggcgat ccggccggac
      481 aggcgtgggc cagcggcatc ttcggcgcgt acaccgcgct gatctacggc acctgcatct
      541 tcggcggctg ggtcgcggac cgggtgatcg gctaccagcg caccatcctg ctgggcgcgc
      601 tggtgatggc ggcgggattg ttcgtgctga tgctgcccag ccgcacgatg ctgctgctcg
      661 gcctcgcgct ggtgatcgtc ggcgatggcc tgttcaagcc caacatctcg acgatggtcg
      721 ggcagcttta cggacgcgac gatccacgcc gcgaccgcgg cttcacgctg ttctacatgg
      781 gcatcaacgg gggcgccctg ctcgcgccgc tgctgaccgg ctggatggcc gcgcacttca
      841 ccgccactcc gatgcagcag aacttccggc tggttttcgc ggccgcgggc attggcatgc
      901 tgatcagcct gctgtggttc tggttcgggc ggcggcagtt gaagcacgtg gggcggcccg
      961 ccgcgggctc cgaaggccct gcgcgcgtgg catgggtggt gctgggcgtg ctcgtgtcgg
     1021 tgccgctggt gttcctgctg ctctcgcggt tgggcgccac gggcctgcaa tggctgctgg
     1081 gactgctgtt cgtcgtggtg gccgcgatgt tgctggtgga ggcggcgcgc catggtcgcg
     1141 tccaagtgca tcgcgtggtc gcgatgctga tcatcttcgc gttcaacgtg ctgttctgga
     1201 tgttctacta ccagctcggc acgtcgttca atttcctcgc cgaaaacatc gtggaccgcg
     1261 ttatgccggg cggctggatc tttccggtcg gctggttcca atcggtgagc ccgctggcga
     1321 tcatcgtatt cgcgccgctg gtgacattga cgtgggcgtg gctggaccgg cgccgcatgg
     1381 agccatcgat cccgcgcaaa ttcggtctcg gactggtctt caacagcctc ggcttcctgg
     1441 tgctgatgtt cgcgctgtcg aagctgctcg gcgcgaatgg gctgattccg ttctggccgc
     1501 tggtgctgtg ctacgtgatg cagaccctcg gcgagctgtg cctgtcgccg atcgggcttt
     1561 cgatggtgac caagctggcg ccaccgcggc tggtgggatt cgcgatggga ggctggttcc
     1621 tgtcgctgtc ggttggcggc aacctttccg gcctgttcgc cagccgcatc gccggtacgg
     1681 gcggcatgac cgcggggtcc gccttgtccg gtttcacctt cgggttctgg ctgctgctgg
     1741 gcgcgggcgt gctgttgctg ctggtctcgc cgctggtcaa ccggctgatg cacggggtgc
     1801 gttagcttcg cccgccatca gcttgtcgag gatgcgcgtc agcgagcgcc gctcctcgtc
     1861 ttccaggcgt tccaggagct cgcgctcgtg gcggcgcgcg agcggcgcaa ccgcgtcgtg
     1921 gatccgccaa ccggccgccg atagccgcag caccgaacgg cgcttgtcgc cgtggtgggt
     1981 gcgacgcagc acacgcccct tcatcaccag gcgcgcgagc gcgcggctga tcgccacctt
     2041 gtccatcgcg gtcagccgcg ccaccgcgcg tgccgacaag ccctcgccgt catagcgcgc
     2101 cagcaccgcc atcactcgcc actcggtggt gccgaggtcg aagcgctgct ggtactcttc
     2161 ggcgatcgcc tggctcaccg tgttggacac tatcgacaac tggtacggaa cgaatttttc
     2221 gagctcgatg ggggcgtgcc tgggcatggc gtcgcaactt gcaaatggtt tcacttgaaa
     2281 ctatcttagc attgctttgc gatcgtcctt gatcgcggcc tcgccggaac tttccgcaac
     2341 cgtcaggccc ctccggccag gctccaccgg aacggccttc cgtggcctga cttttcataa
     2401 aagcccgctt tcacgggacc ttcggagaca aacatgaacg cacagcccaa cgccggcatg
     2461 cagccgacca cgttcgagaa cccgatgggg gtggatggct tcgaattcgt cgaattcgcg
     2521 gggcccgatc cgaagtcgct gcacgacctg ttcaagcggc tgggcttcac ggccgtcgca
     2581 cgccacaagt cgaaggcggt gacgctgtac cggcagggcg cctgcaattt cctgctaaac
     2641 gagcagccgg attcgttcgc gtccgacttc gtgcaaagcc acggaccgtg cgcgtgcgga
     2701 ttcgcgatcc gcttttcgcg cccggcgtcg gaagtcctcg aggccaccct cgccaatggc
     2761 gccaaggaaa tcacccaccg tgcccacacg cgcgcagtcg atgcgccggt gatcgagggc
     2821 atcggcggct gcatgcttta cctcgtcgac cgctacggcg gcaagggcag cgtgttcgat
     2881 gactacgaat ggctggtgcc ggaaggccag cgacagcccg tgggtttcgg cctgaccttc
     2941 atcgaccatc tcacacacaa cctgcacttc ggcgagatgg ccaagtgggc cggctattac
     3001 gagcggctgt tcaacttccg cgagatcaag tacttcgata tcaagggcgc caagaccggg
     3061 ctggtctcca aggcgatgac cgccccggat gggatggtcc ggatcccgct caacgaatcg
     3121 aacgatccga aatcgcagat caatgaatat ctcgacgaat acaagggcga gggcatccag
     3181 cacatcgcgc tcttcaccga caccatctat gacaccgtgg agaagatgag ggcggccggt
     3241 gtcgagtttc tcgacacgcc cgacgcctat ttcgaggtga tcgacgtgcg ggtaccgaac
     3301 cacggcgagg acgtgcccag gctcgcgaag aacaagatcc tgatcgacgc cgacatggaa
     3361 accaagcgga agctgctgct gcagatcttc acccagaact gcatcgggcc gatcttcttc
     3421 gagatcatcc agcgcaaggg caacgaaggc ttcggcgagg gcaacttcca ggccctgttc
     3481 gaatcgatcg agcgcgacca gatgaagcgc ggggtgctgt aggaatttcg accaagctcg
     3541 cggctttgca gggcgggcat tgtgcgccga ccgatgggtc ggcgatgccc ggcctacact
     3601 gaagcgcctc tgcgcggagc gtggcgaacc tcgaaaggtg agcaccagca cgagcaaaat
     3661 gggcaggcgc ttcgactccg cggcctgcgg ccgctacgct cagcgcgaac ggatgattga
     3721 gcatcgacat gatcatgtct gactcacctg cctacctcac cggtttcggc aacgagcacg
     3781 caagcgaagc gctggcaggc gcgctgccgg ttgggcagaa ctcgccgcag aaggtgccgt
     3841 atggactgta cgccgagcag atgtcgggta cggcgttcac cgcgccgcgc cacgccaacc
     3901 gccggagctg gctgtaccgg atccgaccgg ccgcggtgca cggcgccttc gaagcgtatt
     3961 cgcagccgcg cttccacaac cggttcgacg aagcgccgac gtcgcccgac cagatgcggt
     4021 ggagcccctt ctcgctgccg gacgcaccca ccgatttcgt cgacggcctg ttcgcgatgg
     4081 ccggcaacgg cgcgcccgcc atgcaggccg gggtcggcat ccacgtgttc gcctgcaaca
     4141 agcccatgca aggccggttc ttctacgacg ccgacggcga gctcgtggtc gtgccgcagc
     4201 aggggcggct ccggatctcg accgagctcg gcgtgctcga ggtcgagccg caacaaatcg
     4261 cgctggttcc gcgcggcctg cgctttcgcg tcgaccccat ggagggcacc gcgcgcggct
     4321 acgcctgcga gaacttcggc gcgccgctgc ggctgccgga gctcgggccg atcgggtcga
     4381 acggcctggc caatgcatgc gatttcgagg ccccggtcgc ggcctacgaa gacgtcgaag
     4441 gcgatttcga gttgatcgcg aagttccagg gcaatctctg gcgcgcgccg atcgatcatt
     4501 cccccctcga cgtggtggcg tggcatggca cgcacgtgcc gtaccgctac gacctgcgac
     4561 gcttcaacac catcggctcg atcagctatg accatcccga cccgtcgatc ttcaccgtgc
     4621 tgacttcgcc cagcgatacc cccggcaccg cgaacatgga cttcgcgatc ttcccgccgc
     4681 gctggctggt ggccgaacac accttccggc cgccgtggtt ccatcgcaac atcgccagcg
     4741 aattcatggg cctcatccac ggcgaatacg atgccaagga cgccagcgcg ggcggcggat
     4801 tcgtgccggg cggcgccagc ctgcacaatt gcatgagcgg ccacggcccc gacgcggcca
     4861 gctacgacaa ggcgctggcc gtcgataccg cgaagccgca gcacgtcgtc gacaccatgg
     4921 ccttcatgtt cgaaacgcgc aacgtgatcc atcccacccg gcaggccctg gaatcgccgc
     4981 aacgccagcg ggactaccag cagtgctggc aaggactcga gaaacacttc cacccgtcgc
     5041 aacggtaagg cacgaggaga ggcatgaaac tggctacgct caaagaaggt ggcagggacg
     5101 gcacattgat cgtggtcagc cgcgacctcg gccacggcgt gcgcgcgacc ggcatcgcga
     5161 cgacgctgca gcaagcgctc gatgactggc aactcgccgc gccacgactg cacgcgctgt
     5221 acgacgcctt gtgcctgcac cgccacaagg cagtggaata cgccttcgat ctcgatttca
     5281 acgcgctggc cgcgccgctg ccccgcgcct acgagttcgt ggacggcagc gcctacctgc
     5341 cgcacgtgga gcgggtgcgc aaggcgcgcg gcgccacggt tccggagacc ttctacaccg
     5401 atcccttgat gtaccaggcc accagcgccg gcttcctcgg gccgcgcgat cccgtgcgtg
     5461 tccccagcga ggactacggc atcgatctcg aggccgaggt cgtggtggtc accgacgacg
     5521 tgccgatggc ggtgacgccg cagcaggctt cgcaccacat ccagttgctc ggcctggtca
     5581 acgacgtgtc cctgcgcagc ctcattccgg ccgagctggc caagggcttc ggcttcctgc
     5641 aatcgaagcc gcgctcggcg ctgtcgccgg tcctggtgac gccggacgaa ctgcgcgatg
     5701 cctggcgcga cgacaaggtg cacctgccga tgcgcacgtg gatcaacggc cagtggttcg
     5761 gcgaagccga ggccggcgaa gacatgcaat tcaacttcgc acaactggtc gcgcacgcgg
     5821 cacgcacccg cccgctcacc gcgggcacca tcgtcggctc cggcacgatc gccaaccagg
     5881 acacgtcgcg cggcgcatcg tgcctagccg agcagcgcac ggtcgagaaa ctgcgcgatg
     5941 gcgaggccag gacgccgttc ctgaaatacg gcgacacggt caggatcgac atgcacgatg
     6001 ccgacggcaa ttcgatcttc gggtcgatcg agcagcggat cgagccgggc tgaaccgtgc
     6061 gttcgtccat gaacgctgtt tcggcccgtg tacacgcggg gtgcggcact tcgcattttt
     6121 tcgcgccaga acggccatcc atggcctgac tttccacaac agccgctccg caggctgcgt
     6181 tagacttggc ccctggattc gcagcgcccc gggcgccaaa tacgacatgg gatcgcgttc
     6241 gccacaatct gcaggctacg catcatcgga gcgccacacg ggcgttccgg ccaatgacgg
     6301 cgatgccgag cgcttctgct cgagctgcgc gttcgccgag gcctgctcgg tcgccggcta
     6361 cggcaagacg gaactgtccg agctgcactg cctggtcgag cacgtgggcc cgttcgaacc
     6421 cggccagcgg atcttccgga acggcgaccc gttccgcgcg ctcttcgcgg tgcgctcggg
     6481 gatggtcaag accacgatgg ttgacgcgga agggcgcgag caggtgctcg gcttctacct
     6541 gccgggcgag atggtcgggc tcgacgcgat ctatcccgaa cactacccct gcgacgggat
     6601 cgcgctggaa accaccgagt gctgccggtt ctcctttccc gcgatgagcg ccctcgccgc
     6661 acgccagccc aaggtccagc agcacctgtt ccggatgctc agcaaggaac tgggttcggc
     6721 cacgctgctc gccggcgacc actctgccga cgaacgcatg gcggcgttcc tggtcgacct
     6781 cggcaaccgc tacgccgcgc gcggcctgtc cggcaccgag ttccgcctca gcatggcgcg
     6841 cgccgacatc gccaactacc tgcggctcgc cgccgaaacc gtcagccgcg tgctcggccg
     6901 gttccgcgaa cagaagctga tctcgatcga tggccgccgg gtggagctgc ttgatccggc
     6961 gcgcctgcgc gaactcgccc gcagcgtgct gaacacgtag ggatcgttcg tgaccgccct
     7021 gcgcggttcc gttttgcacc ggttgcgcgg cgtcaacgcg cgccaatgac gcgtccgcga
     7081 tactcggccg accttccact ccgtgtaccg ccatggctgc ctggtacctg cagatcctca
     7141 tgatccacgt cggctgcgtg attgcctccg gcaccttgtt cttcacccgc ggctgcatga
     7201 tgctggccgg cctggcggcc gccaaccaca tcgcgttgcg ccgcatttcg gtggtgatcg
     7261 acacgctgct gctggccgcg gcgattgcgt tgaccacgat catccaccaa tacccgttcg
     7321 tgcaggcgtg gctgaccgtg aaggtgctgc tgctggtggt ctacatcatg ctgggggtgt
     7381 tcgccctgcg ccgcgggcgc acccgccgga cccgcgccgc ctgcttcggc gcggcaatgc
     7441 tggtattcct tttcatcgtc accgtggcgc gcacccacag cccgctggga gcattcgcga
     7501 attcgtaggg cgcgccgcgc aggccggttc aaggggcgca gcgacggaac cggcggcctc
     7561 ggcgggccgc tcggccggca tgcgatcccc cggcatcgcg caagcgcccg ttttcgccct
     7621 acgcccgcgt caccagccca tcgcgcacct gcacggccac cgccaccagc gattggccgc
     7681 ggcaaggccc ggccacgcac aagccgccct cgcggagcgc gaacgtggcg ccgtgcacgg
     7741 cacaggtcag cttgtcgtct ttcaccagga acagccccgg cgcgtagtcc atccgccggc
     7801 ccgcgtgcgg acagatgttc agccacgcct gcaccgaact gccgcgccga gcaagcacca
     7861 gcggctcccc atcgggatcg ggcggactga cgcccagcac gccgccgtcg gggatgtcct
     7921 cgaggcggca aagggcttcc cgggggctcg tcatgtccat ccgcgactcc tagccgattt
     7981 gaccgggcgc accgactgcg gagcctccgg cgcaccggcg ggcgggccgc ggaagcacct
     8041 ccgcggctgc ggaccttgcg actccggccc tgcgcccgca tggtagaggt gtcattctgc
     8101 cacgcggacc catcccatgc gcgcgcgagc gtttaccttg ccgctgcccc ggcaccccct
     8161 gctgcgcatg ctggtgctcg tgggcggcgc gatcctgctg gccgggctgg tcgcgatgag
     8221 cctgatcgtt ggcaccgccg tattggccgt gggggccacg tggctgctgg tgcggcgctg
     8281 gatcgggcgc cgcaagcgcc cggccgatcc cgcgatcatc gaaggcgaat tcaccgtggt
     8341 gcaacgcaat tccctgccgc gcacggactg aacggcatca cgacaggatc cggatggcgg
     8401 gggcgatcgg caatggccat catgtccgca tgaacgccaa gcccaacatc gatgccggcc
     8461 gcgatccggt tgcccgggcc cgcgacatcc tggaccacag cgaccccgcg cccacccttg
     8521 ccgaactggc cagccgggtg ggcctctcgc ccgcgtggct gcaacgcagt ttccgcaggc
     8581 gtttcgggat ctcgcccgcc gaattcgtac gggcacgccg cttcggtgag ctcaagcggg
     8641 cgctgcgcca gggctccgac gtcaccgacg ccgtatacgc cgccggcttc gggtccggca
     8701 gccgcgtgta cgagcacagc gaccgcctgc tcggcatgcc accggcgcgc tatcgcgaag
     8761 gcggtgcagg catcgccatt cgttacacga ccatggactc gccgctcggc cacgtgctgg
     8821 tcgcggcaac cgaacgcggc ctgtgcgcgg tgaccgtggg cgacgacgat gcccaactgc
     8881 tcgccgcgct gcgcaaggaa tttccgtccg ccgaagtgac gcgcgtcgat gccggccgcg
     8941 acgaatggct ggccgcggtg gtcgcgcgtg tcgcgcggca gttcaatcgc cgcacgggcg
     9001 cggcagcgtc gccggcgcta ccgcccctgg acatcaccgc caccgctttc cagtggcggg
     9061 tgtgggaagc gctgacccgc attccggcgg gcgaaacccg cagctatgcc gagatcgccc
     9121 gcgccatcgg ggaacccggc gcagcacggg ccgtcggtcg cgcctgcggc caaaacaaac
     9181 tggcgctgat cgtgccttgc caccgggtgg tgcgtgcaga cggaaccctc ggcggctggc
     9241 gctggggcgc gacccgcaag caggcactcc tcgaccgcga acgccgcgcc ggcgcgcgtt
     9301 gaacctccct caacacggcg tcgcgaaact ttcatgacgg cgcagcgatc cgcgccgtgc
     9361 cgccggatct agaattgcat tgcgcctccc catgcaggag gcgtcttttg tttctccgcg
     9421 ccgaacgcac cgcgcaaggt catcccatga acgaagtcgc cattcccgct gccgcaatcg
     9481 cggcaccgca acggcgttgg ctgataccgc tggcgctgct ctcggtgtac gtgatctggg
     9541 gctcgaccta cctcggaatc cggatcgggt tgaccgggtt tccgccgttc atgctggcgg
     9601 cgatccgttt cttcgcggcc ggcatcgcga tgtacgcgtg gctgcgcctg cgcggcgtga
     9661 agccgccgac gcgcaggcag tggcgcaacg cggcgatcac cggcgtgctg ctgctgttgc
     9721 tcggcaacgg catggtctgc ttcgccgaac agagcgtgac ctccggcatc gccgcgatcg
     9781 ccatcgcgag cgaaccgctg ttcatcgcgc tgttcctggt cgcctaccgg gaacgcccga
     9841 gccgcggcga gatcgtcggg ctggcgatcg gattcgtggg cgtcgtcgtg ctgaacagcg
     9901 gcggcgcgat gcgcgccgcg ccatgggccg ccggggcgct gctgttggcc accatcgcct
     9961 gggcgttcgg ttcgatctgg agccgccacc aggacatgcc cgaaggctcg atgaacgtgg
    10021 ccgcgcagat gctatgtgcg gcgcccgcgc tggcattgac cgcttggctc accggcgaac
    10081 gcctgcccgc gcatccgccc gccgcttcga tcctggcgat cgcgtacctg gccgtgttcg
    10141 gctcgatcat cgccttttcc gcgtacctgt tcctggtcaa gcacgcgcgg ccgacgctga
    10201 ccgcgagcta cgccta
//
LOCUS       DASSNH010000308         8581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_552919, whole genome shotgun sequence.
ACCESSION   DASSNH010000308 DASSNH010000000
VERSION     DASSNH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552919"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="VFK00_11540"
     CDS             <1..1522
                     /locus_tag="VFK00_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7593619.1"
                     /translation="LGALVAGTKYRGDFEKRLKAVIAQLKKDPNAILFVDEIHTIIGA
                     GSASGGTMDASNLIKPVLASGELRCIGSTTFQEFRGVFEKDRALARRFQKIDVVEPSV
                     ADTVEILKGLKSRFEEHHDVSYTEEALKAAVDLSVKHIPDRLLPDKAIDVIDEAGAHQ
                     RLLPQDERTGKVDVGEVEYIVARMARIPQKQVSASDRDVLRNLDRNLKMVIFGQDQAI
                     DTLASAIKMARSGLGDPNKPIGNFLFAGPTGVGKTEVTRQLAMQLGIELVRFDMSEYM
                     EPHSVSRLIGAPPGYVGFDQGGLLTESVVKHPHCVLLLDEIEKAHPDLFNILLQVMDH
                     GVLTDTNGREANFRNVIVVMTTNAGARQAASRSIGFMEQDRTTDAMEVIRRTFTPEFR
                     NRLDAIVQFKALDMEHILRVVDKFLIELETQLLEKRVSLNVEPAARQWLAEHGFDQQM
                     GARPMARLIQDKVKRAIADELLFGSLADGGKVTLDARDGELTVDTAAAEKLPAVVE"
     gene            complement(1652..2644)
                     /locus_tag="VFK00_11545"
     CDS             complement(1652..2644)
                     /locus_tag="VFK00_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET7593620.1"
                     /translation="MSRYIAERAVLGTQPLVVLRDDAGRRVRIACHGAALIGFEVPRG
                     GAPFDIAFGYHDDAEIESRRGSHFAIMVPFAGRIADARYRFDGREYDLQPGVEGAARG
                     VMHGFLRDADFGVAELTAGESSARATLGAAIQPRAGYPFTLDVAIAFMLDAGGLALEV
                     RMRNAGDTAAPCFFGWHPYLRVNDREVDGWLLEIPADTLIRTDPDMIALEGNAAYVSL
                     DDAPAFDFRQPREIAHSILDAGYSDAEPGPGGHIRTRLTDPADGFAVTMWQERGGVHA
                     FTADTLKHGARTAVALEPMESMANAFNRPECAGTIRLEPGAERVFRCGIEVTGG"
     gene            2875..3480
                     /locus_tag="VFK00_11550"
     CDS             2875..3480
                     /locus_tag="VFK00_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593621.1"
                     /translation="MNGNVANPWPLGFGVLGIALWMLSVIPAGIAGPMGFGLGPGAMH
                     GIFTVAMLGLLIAGIAAFLRRESWLGFFFLLWSGLFWASGFGFERAGGMRFEGWYLMT
                     ITLVNFYLWFAAWRSDKLGGAVSLMVLLLWVSFLFYGLNGFFGGWALLRIGGAFGLAS
                     ALASFYVSATTLAVTHYPDRRYPGILYVETYPGTPPREPRI"
     gene            complement(3526..5043)
                     /locus_tag="VFK00_11555"
     CDS             complement(3526..5043)
                     /locus_tag="VFK00_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7593622.1"
                     /translation="MKFAPLLLAAVCLLPALTGCARATAAEPAGAPTLAIGSKNFTES
                     VVLGEIARLSARERGVQVQHRRSLGGTRILWRALTEGDIDAYPEYTGTITHELLRDLP
                     PDARLAVLRARLKQLGIGITDPIGFNNTYAIGMLESEAAKLHVRDISDLANHPGLKLA
                     FSNEFMDRADGWPGLKQAYHLPQIARGMDHDLAYRALASGAVDAIDLYSTDANIDYYH
                     VRVLKDDRHYFPHYDAVFLYRLDLKRRAPAFVEALQDLAGSIDAKQMRAMNKAVQIDG
                     ESETTAASDFLGVQVKPGGGTAAFWSRIARYTWQHLALVGISLSLAIALAVPLGIFAS
                     RYPRLGQLVLGLTGILQTVPSLALFVFMIPIFGIGAEPAIAALFLYSLLPIVRNTHAG
                     LTGISPSLLESAAALGLPPRVRLWRIELPLALRSILAGVKIAAVINVGTATLGALIGS
                     GGYGQPILTGIRLDSVPLIMEGAVPAALLALVMQGLFESIERVLTPRGLRLRAQR"
     gene            complement(5040..5807)
                     /locus_tag="VFK00_11560"
     CDS             complement(5040..5807)
                     /locus_tag="VFK00_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7593623.1"
                     /translation="MPASRDSDSAFTLVGVTRAWGEVRAVDDVSLDFDRGRTTILIGS
                     SGSGKSTVLRLLIALEWPDAGQVLFDSEPLQPTRVLAVRRRVGYVIQEGGLFPHLTAL
                     GNISLLPHHLGWNSARIRERALELAELTHLKESQFQRFPAELSGGQRQRVALMRALML
                     DPPTLLLDEPLGALDPLVRHGLQDELREIFRGLGKTVIMVTHDLAEAAFFSSRLVLMR
                     RGRVIQDGSIDDFRRAPANDFVREFLAAHRDLPGAAT"
     gene            complement(5785..6555)
                     /locus_tag="VFK00_11565"
     CDS             complement(5785..6555)
                     /locus_tag="VFK00_11565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7593624.1"
                     /translation="MRPQRYHIRFPSANLTSASQSEAYFLITEDGEERRIDFHDYAEI
                     YKHPGLYEQLFYQRLKCTSPAKLTELLRSAAGKAGVDPTQLRVLDVGAGNGMVGELLV
                     AHGSARVVGIDNIEEAERAADRDRPGAYDAYYVADLTNLSPELRAELNDWNFDAMTTV
                     AALGFGDIPVAAFAAAYNLVKTDGWIAFNIKETFLETSDDSGFSRLVKRMIHDDYLKL
                     HHLERYRHRLSIEGNMLYYYAIVGQKRRDIDDACLAGL"
     gene            complement(6912..7265)
                     /locus_tag="VFK00_11570"
     CDS             complement(6912..7265)
                     /locus_tag="VFK00_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593625.1"
                     /translation="MTAPRAGSAGATRACPHCRETILESAAVCPACNHKLRFGGPAGD
                     LGAQAELTPLRVEGSFRNPDSGAWEYSMVLTIRNERGEEIARRLVGVGAMQPGEQRTF
                     SLSVEMNPAGKRTRH"
     gene            complement(7262..7807)
                     /locus_tag="VFK00_11575"
     CDS             complement(7262..7807)
                     /locus_tag="VFK00_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7593626.1"
                     /translation="MALWKEPVKSSSSLPNPPDVARYDAPEPVETPVHTKAPAAGYEA
                     KSVPGASKSGSESHIAPDLVIEGKIEGAGHVRIAGRFKGDINVRGDLTIENGAKVNGS
                     VRAERVTIAGELTGNIETAAHVELLQTGSLTGDVKSTTFSVAKGSKMRGQAEFGWDDG
                     KAGVTDISRNNGHANGNGHGK"
     gene            8223..>8581
                     /locus_tag="VFK00_11580"
     CDS             8223..>8581
                     /locus_tag="VFK00_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7593627.1"
                     /translation="MRFLSLVRVNEGSGKKPDERLVNDMTQLIAEMTADGTLLDTAGL
                     CPTSQGKKVRLSRGKQAVSDGPFTESKEEIGGYALLQADSMDHALQLTRRFLDVHGGE
                     WEVECELRQIAEHRDDAT"
BASE COUNT         1420 a         2808 c         2922 g         1431 t
ORIGIN      
        1 cctcggcgcg ctggtcgcgg gcaccaagta ccgcggcgat ttcgagaagc gcctgaaggc
       61 cgtgatcgcc cagctcaaga aggatcccaa cgcgatcctg ttcgtggacg aaatccacac
      121 catcatcggt gcgggttcgg cctccggcgg caccatggat gcctccaacc tcatcaagcc
      181 ggtgctggcg tcgggggagc tgcgctgcat cggctcgacc accttccagg agttccgcgg
      241 cgtgttcgag aaggaccgcg cgctggcgcg gcggttccag aagatcgacg tggtggaacc
      301 ctctgtcgcc gataccgtgg aaatcctgaa ggggctgaag tcccgattcg aggagcacca
      361 cgacgtcagc tacaccgagg aagcgctgaa ggccgcggtc gatctgtcgg tcaagcacat
      421 tcccgaccgc ctgctgccgg acaaggccat cgacgtgatc gacgaggccg gcgcccacca
      481 gcgcctgctg ccccaagacg aaaggaccgg caaggtcgac gtgggcgagg tcgagtacat
      541 cgtggcgcgg atggcgcgga tcccgcagaa acaggtttcc gcctccgatc gcgacgtgtt
      601 gcggaacctc gaccgcaacc tgaagatggt gatcttcggg caggaccagg cgatcgacac
      661 cctcgcatcg gcgatcaaga tggcacgctc gggcctgggc gatcccaaca agccgatcgg
      721 caacttcctg ttcgcgggcc cgaccggcgt cggcaagacc gaggtcaccc gccagctcgc
      781 gatgcagctc ggcatcgagc tggtgcgctt cgacatgtcc gaatacatgg agccgcattc
      841 ggtgtcgcgc ctgatcgggg cgcccccggg ctacgtcggc ttcgaccagg gcggcctgct
      901 caccgaatcg gtggtcaagc atccgcactg cgtgctgctg ctcgacgaaa tcgagaaggc
      961 gcacccggac ctcttcaaca tcctgttgca ggtgatggac cacggcgtgc tgaccgacac
     1021 caatggccgc gaggcgaact tccgcaacgt catcgtggtg atgacgacca acgccggtgc
     1081 gcgccaggcc gcgagccgca gcatcgggtt catggaacag gaccgcacca cggacgcgat
     1141 ggaagtcatt cgtcggacct tcacgccgga gttccgcaac cgcctggacg ccatcgtgca
     1201 gttcaaggcg ctcgacatgg agcacatcct gcgggtggtc gacaagttcc tgatcgagct
     1261 cgaaacccag ttgctggaga agcgcgtcag cctgaacgtc gagcccgcgg cgcggcagtg
     1321 gctggccgag cacggcttcg accagcagat gggcgcacgc ccgatggcgc ggctgatcca
     1381 ggacaaggtc aagcgcgcga tcgccgacga gctgctgttc ggcagcctcg ccgacggcgg
     1441 caaggtaacg ctcgatgcgc gcgacggcga gctgaccgtc gacaccgcgg ccgccgaaaa
     1501 gctgccagcg gtcgtggaat agcctccgat gcatccttcc cccgcctgcg ggggaagctt
     1561 cccgaagggt ggtcgggctg atgcgcacga cgtgcgctgt tcagccaccg aataatcgcg
     1621 gcaaggccga ccacaggtta gccgcgcgcc atcagccgcc cgtcacctcg atgccgcagc
     1681 gaaacacgcg ctcggccccc ggttcgagcc gtatcgtgcc cgcgcattcg gggcggttga
     1741 acgcattcgc catgctttcc atcggttcca gggcaaccgc ggttcgcgcg ccgtgcttca
     1801 gcgtgtccgc ggtgaaggcg tgcacgccgc cgcgttcctg ccacatcgtg acggcgaagc
     1861 catcggcagg atcggtgagc cgcgtgcgga tgtgaccgcc tgggcccggc tcggcatccg
     1921 agtaacccgc atcgaggatg ctgtgcgcga tttcccgcgg ctggcggaaa tcgaacgccg
     1981 gtgcgtcgtc cagcgacacg taggctgcgt ttccctccag cgcaatcatg tccgggtccg
     2041 tgcggatcag ggtgtcggca ggaatttcca gcaaccagcc gtccacctcg cgatcattga
     2101 cgcgcaggta cgggtgccag ccgaagaagc acggtgccgc ggtgtcgccc gcgttgcgca
     2161 tgcgcacttc cagcgcgagg cccccggcgt ccagcatgaa agcgatggca acgtcgagcg
     2221 tgaagggata gcccgcgcgc ggctggatcg cggcgccgag cgtggcgcgt gccgacgatt
     2281 cgcctgccgt gagttccgcg accccgaagt ccgcgtcgcg caggaagccg tgcatgaccc
     2341 cgcgcgcggc cccctcgacg ccaggttgca ggtcgtactc gcggccgtcg aagcgatagc
     2401 gcgcatcggc gatccgaccg gcgaagggca ccatgatcgc gaagtgcgac ccgcggcgcg
     2461 actcgatctc cgcgtcgtcg tggtagccga atgcgatgtc gaacggcgcg ccgccgcgcg
     2521 gcacctcgaa gccgatgagc gccgcgccat ggcaggcgat ccgcacacgc cggccggcgt
     2581 cgtcgcgcag caccaccagt ggctgcgtcc cgagcacggc acgttcggcg atgtatcggc
     2641 tcatcgactg gcccgtttgc gacacaggga gtgtaacaag ccgtcgatga ggttccggtt
     2701 tcaccggcac gagtgcgcca tccttgacgg caccttgaca agccgctgac tagattggac
     2761 tccggagggc gcccatcgcc gcgcttccat cgacgtctgg cgacagcgcc tgtcgcgcac
     2821 cgcccgcgat ccgcgggacc cggtgcgatt ccctggaatc gcaggaggtg cctcatgaac
     2881 ggcaacgtcg ccaacccctg gccgctcggt ttcggcgtgc tgggcattgc gctctggatg
     2941 ctttcggtga tccccgccgg catcgccggt cccatgggct tcggcctcgg tcccggcgcc
     3001 atgcatggga tcttcaccgt cgcgatgctc ggcctgctga tcgccgggat cgccgcattc
     3061 ctgcgccgcg aaagctggct cgggttcttc ttcctgttgt ggtccggcct gttctgggcg
     3121 agcggtttcg gcttcgagcg cgcgggcggc atgcgcttcg aaggctggta cctgatgaca
     3181 atcacgctgg tgaacttcta cctctggttc gcggcgtgga ggagcgacaa gctcggcggc
     3241 gctgtttccc tgatggtgct gctgctgtgg gtttcgttcc tgttctacgg attgaacggc
     3301 ttcttcggcg gctgggcgct gctccggatc ggtggcgcct tcgggctcgc gagcgcgctg
     3361 gcatcgttct acgtctccgc caccacgctg gcggtcaccc attacccgga ccggcgctac
     3421 ccgggcatcc tgtacgtcga gacgtacccg ggcacgccgc cgcgcgaacc gcggatctga
     3481 acgtgcgagg tttccgcggc ggctacctgc cgccgcggaa accggtcatc gctgcgcgcg
     3541 caaccgcaac ccgcgcggcg tcagcacgcg ttcgatgctt tcgaacaagc cctgcatcac
     3601 cagcgcgagc agcgccgccg gcaccgcgcc ctccatgatc agcggcacgc tgtcgaggcg
     3661 gatgccggtg aggatcggtt gcccatagcc ccccgagccg atcaatgcgc cgagcgtcgc
     3721 cgtcccgacg ttgatgacgg cggcgatctt cacgcccgcg aggatcgagc gcagcgccag
     3781 cggcagttcg atccgccaca gtcgaacccg gggcggcagg cccagcgccg ccgccgattc
     3841 cagcagcgac ggcgaaatgc cggtgagccc cgcatgggtg ttgcgcacga tcggcaacag
     3901 gctgtagagg aacagcgccg cgatcgcagg ctccgcgccg atcccgaaga tcgggatcat
     3961 gaagacgaac aaggcaagcg agggcaccgt ctgcaggatg ccggtcaagc cgagcaccag
     4021 ctgccccagg cgcgggtagc gcgacgcgaa gataccgagc ggcacggcca gcgcgatcgc
     4081 caacgacagc gaaatgccga ccagcgcgag gtgctgccac gtgtagcgcg cgatccggct
     4141 ccagaacgcc gcggttccgc cgccgggctt cacctgcacc ccgaggaaat ccgacgcggc
     4201 cgtggtctcg ctctctccgt cgatctgcac tgccttgttc atggcgcgca tctgcttcgc
     4261 gtcgatgctg cccgcgaggt cctgcagcgc ctcgacgaag gcgggtgcgc gtcgcttcag
     4321 gtccaggcgg tacaggaaca ccgcgtcgta gtgcgggaag taatggcggt cgtccttcag
     4381 cacccgcacg tggtagtaat cgatgttggc gtcggtgctg tacaggtcga tcgcatcgac
     4441 cgcgccggag gcgagcgcgc ggtaggcgag gtcgtgatcc atgccacgcg cgatttgggg
     4501 caggtggtag gcctgcttga ggcccggcca gccgtccgcc cggtccatga attcgttgga
     4561 gaacgccagc ttcaggccgg gatgattcgc gagatcggaa atgtcgcgga cgtgcagctt
     4621 ggccgcttcg gattccagca tcccgatcgc gtaggtgttg ttgaagccga tgggatcggt
     4681 gatgccgatg ccgagttgct tcaaacgcgc gcgcagcacg gcgagcctgg catcgggcgg
     4741 caaatcccga agcaactcgt gggtgatcgt gccggtgtat tccgggtagg cgtcgatgtc
     4801 gccttcggtc agcgcgcgcc acaggatgcg ggtgccgccg aggctgcgcc ggtgctgcac
     4861 ctgcaccccg cgttcgcgcg cggaaagccg cgcgatctcc cccagcacca ccgattcggt
     4921 gaagttcttg gagccgatcg ccagtgtcgg cgctcccgcg ggttccgcgg cggtggcacg
     4981 cgcgcaaccg gtcaatgccg gcagcaggca cacggcggcc agcaggagcg gcgcgaattt
     5041 catgtggcgg caccgggcag atcgcggtgc gccgccagga attcgcgcac gaaatcgttc
     5101 gcgggtgcgc gccggaaatc atcgatgctc ccgtcctgga tcacgcgacc gcgccgcatc
     5161 agcacaagcc ggctgctgaa gaacgcggct tcggcgagat catgggtcac catgatcacg
     5221 gtcttgccga gccctctgaa gatctcgcgc agttcgtcct gcaggccgtg acgcaccagc
     5281 gggtcgagcg ccccgagtgg ttcgtccagc agcagggtgg gcggatccag catcaaggcg
     5341 cgcatcagcg ccacgcgttg gcgctggccg cccgagagtt ccgccggaaa gcgctggaac
     5401 tggctttcct tgaggtgggt cagttcggcc aattccagtg cgcgctcgcg gatccgtgcg
     5461 ctgttccagc ctaggtggtg cggcagcagg gagatgttgc cgagtgccgt gaggtgcggg
     5521 aacaggccgc cttcctggat cacgtagccg acccgccgcc gcactgccag gacacgcgtg
     5581 ggttgcaacg gctcggaatc gaagaggacc tggcccgcgt ccggccactc gagcgcgatc
     5641 agcaggcgca gcacggtgga tttacccgag ccgctgctgc cgatcaggat ggtggtgcgc
     5701 ccgcggtcga agtcgaggct gacgtcatcg acggcgcgca cttcgcccca cgcccgggtg
     5761 acgccaacca gtgtgaatgc cgaatcagag tcccgcgagg caggcatcgt cgatgtcgcg
     5821 cctcttctgg ccgacgatgg cgtagtaata cagcatgttg ccttcgatcg acagtcggtg
     5881 gcggtaacgc tcgaggtggt gcagcttcag gtagtcgtcg tggatcatcc gcttcaccag
     5941 ccgcgagaag ccgctgtcgt cgctggtttc gaggaaggtt tccttgatgt tgaaggcgat
     6001 ccagccgtcg gtcttcacga ggttgtaggc cgccgcgaac gcggccaccg ggatgtcgcc
     6061 gaaaccaagc gccgcgaccg tggtcatcgc gtcgaagttc cagtcgttga gttcggcccg
     6121 cagttccggg gaaaggttcg tgaggtcggc gacgtagtac gcgtcgtacg cgccgggccg
     6181 gtcgcgatcc gcggcgcgct cggcttcctc gatgttgtcg atgccgacca cccgtgccga
     6241 gccgtgcgcg accagcagtt cgccgaccat gccgttgccg gcgccgacat caagtacgcg
     6301 cagttgggtg ggatcgacgc ccgccttgcc ggccgcgctg cgcaacagct ccgtcagctt
     6361 ggccggcgaa gtgcacttga gcctctggta gaacagttgc tcgtacaagc cgggatgctt
     6421 gtagatctcg gcgtagtcgt ggaaatcgat gcggcgttcc tcgccgtctt ccgtgataag
     6481 gaaatacgcc tcgctctggc tggcgctggt gaggttggcg ctgggaaaac ggatgtggta
     6541 gcgctgcggt cgcatgctgc tccttggcat cgtcggtggg gagcgtcgcg acgacggtgc
     6601 aggcagcgcg cggcgctgga ccgggatccc gtcacaggcg ccatgtgcgc gcatggcgac
     6661 gccgacgcac caccgtattg catacccgga ggcattgcaa accgacacag cgaccggtgg
     6721 gcgaatggcg tgacgcaccg atgatcgcgg ccatgcgccg ctcctgcaac agcgggcgtt
     6781 gcacgtgcgt gacgggacgc gttgcaggag cgcgccatgc gcgaacggga agcgtcaggg
     6841 cgggcaccgc ccgccgacgt gtggtgccgg cggacaatat ccgccccacg cgctacgccg
     6901 cggcaacgca ctcaatgccg cgtgcgcttc ccggctggat tcatctcgac gctgagcgaa
     6961 aacgtgcgtt gctcgcccgg ctgcatcgcg ccgacgccga ccagtcgccg ggcgatttcc
     7021 tcgccgcgct cgttgcggat cgtcagcacc atcgagtatt cccacgcccc gctgtcggga
     7081 ttgcggaagc tgccctcgac gcgcagcggc gtcagctccg cctgcgcgcc gaggtcaccg
     7141 gccgggccgc cgaaccgcag cttgtggttg caggcggggc agaccgcggc actttcgagg
     7201 atcgtttcgc gacaatgcgg gcaggcgcgc gtcgcgcctg ctgaacccgc gcgtggcgcg
     7261 gtcatttgcc gtgaccgtta ccgttggcgt gcccgttgtt cctggaaatg tcggtgaccc
     7321 cggccttgcc atcgtcccag ccgaattcgg cctggccgcg catctttgaa cccttggcga
     7381 cgctgaaggt cgtggatttc acatcgcccg tcagcgaccc cgtctgcagc agttccacgt
     7441 gcgccgcggt ttcgatgttg ccggtcagct cgccggcgat cgtcacgcgc tccgcgcgca
     7501 cgctgccatt gaccttggcg ccgttctcga tcgtgaggtc gccacgcacg ttgatgtcgc
     7561 ccttgaagcg gccggcgatg cgcacgtggc ccgcgccttc gatcttgcct tcgatcacga
     7621 ggtcgggcgc gatgtgcgac tcggagccgg acttgctggc gccggggacg gacttggctt
     7681 cgtagccggc ggccggcgcc ttggtgtgca cgggggtttc aacgggttcc ggggcgtcgt
     7741 agcgggccac gtcgggcgga ttgggcagcg acgaggagga tttcacgggt tctttccaca
     7801 gggccatggc ggcgctccgg tttcagggtc aggggtggcc gagcgtaccg ccaaatcgtg
     7861 cggatgggaa ccgaaaaatg aacagaaatg cgagtccgtt cgcggaattg tggactgcat
     7921 ctcttgtaaa gcctgaacag gtgtgccacg agcgtgccgc caaccgtgac agcagcccgg
     7981 ataaagcaaa gcgacttcgt ttgcgggact gcaaacgcga acggcggagc cggcccgaag
     8041 ggcgagcgac aaggatgtcg cgagtcaatc cggggacccg ccccatcggc cccgaattcc
     8101 atgcttcgcg gttgcacccg gactatacgg ctgtcgaatc gatgactgct cgttcgtcgc
     8161 ggtatcagga gcgtagcact gccggcatcc gcccgtagcg gcttcgacct gaggagacac
     8221 gaatgcgatt cctatccctg gtccgggtca acgagggttc gggcaagaag cctgacgaga
     8281 ggctggtcaa tgacatgacg cagttgatcg cagaaatgac cgcggacggc acgctgctgg
     8341 atacggccgg cctgtgcccg acgtcgcagg gcaagaaggt gcggctctcg cggggcaagc
     8401 aggcggtgtc ggacgggccg ttcaccgagt ccaaggagga gatcgggggt tacgccctgc
     8461 tgcaggcgga ttcgatggat catgcgctgc aactgacccg ccgcttcctc gacgtgcacg
     8521 gcggcgaatg ggaagtggag tgcgaactgc ggcagattgc agaacatcgt gatgatgcaa
     8581 c
//
LOCUS       DASSNH010000309         8505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1061621, whole genome shotgun sequence.
ACCESSION   DASSNH010000309 DASSNH010000000
VERSION     DASSNH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061621"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /locus_tag="VFK00_11585"
     CDS             <1..2269
                     /locus_tag="VFK00_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HET7593628.1"
                     /translation="GINFAAGATIPKRLVSDGGFGFAAHTTAFEDKLGRGTRLTIRCT
                     DARKRADVDLHLSLYDELQALTFEATCTNASQADIAIHSLEPVRAVANEHGVLHASGV
                     SKCLTNGEMYFDAGTVHTFGEREGAITSGHLKGVNLANGPFPGQSETIHSWWNAGLFC
                     GHDRESITLGYLENTLCLGNLLISRPADDRISLLAESAFPPSVTLRPGSSIGSNRLMI
                     EIADDPYRALESYAAAVGTANDARTKSILNGWCSWFYTLSHVSQHEVMANTAFAAENL
                     KPYGLEYIQIDEGFQRWHGDWEGNERFPNGMKWLAQQIRSHGFKAGLWISPYVIEEPT
                     KLFQEHPEWLVKGRDGSPQRIGNWPAGTAPPADENPKKYCLDVTHPGGAKWLHDLVDT
                     IVNDWGYEMIKIDFVFWSILAAERFHDPTMSTAAAYRKGMEIMRRAAGDKCHILECGP
                     GAITTGLIDSMRIEADVNYGFSDTAWETYFTHPASSSSAAAKRYYFHKRTWINDADHL
                     CMDLLTDTESEAAATLIAFSGGNLFSGDRLTQLEPPKLEILKKITPSLGQAAVPVDLF
                     EHEMQYAFAMKVERPFAEWTLLAVFNPDRKEPVTRKFRLDRLGLRPDGTYIAFDFWKQ
                     QLARELRGAEIEVTVEPGSVTLLALHKRSGIPQFVSTDRHVSQGGVEIEDVHWDGATK
                     TLSGTSTGPLHSAHDVFVYVPDALGWTWEGSAFFRDHGAYTLKLVDRHLIRVHANFDT
                     ADQVRWKARYGEFLK"
     gene            2632..3438
                     /locus_tag="VFK00_11590"
     CDS             2632..3438
                     /locus_tag="VFK00_11590"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7593629.1"
                     /translation="MKKKLPLAVAVLGLAGVFSSASATMLNDTYQGSNSHGYQDVIGP
                     STYDVDGMEASLSGSKLTVTIYTNFAGNAGIESQWTKNATGIGYGDLFLSTGWSPNGP
                     ATYANDNAGNGNHWTYAFSLDDALSNTGGTGTIYVLDAGNGSNHNPEVLLSDAFMDCA
                     GRCIYRDGQAVAVNTSMATATNNTGTWTVGNGFLSFSFDTAGLDLDPSNFGFHWTMYC
                     GNDVIEGLGQATAVPEPGPIGLFLLGLGVIGLGAVRQRKAARDMASGQSK"
     gene            complement(3970..5271)
                     /locus_tag="VFK00_11595"
     CDS             complement(3970..5271)
                     /locus_tag="VFK00_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7593630.1"
                     /translation="MSSMFQSLRSHNYRLWAGGALVSNVGTWMQRIGQDWLVLTVLTH
                     HNATAVGTVMALQFGPPLFMLPLSGFAADHLDRRKLLLFTQGTAGLLALGLGLLTVTG
                     VVRLWQVYGFALLLGCVTAFDAPARQAFVSDLVTDDDLANAVALNSTSFNAARMVGPA
                     IAGILIAAVGEGWLFLINAGSYAAVLVSLSFLRVHELHTEARPPRTRGSLLAGFGYVW
                     QRPDLFAVLMMLLLLGTFGFNFSIFISTMSVTVFHGDASQYGLLTSAMAVGTMSGALF
                     SARRPFPGMVLMAVSAAAFAVALAVAAVMPSPFSFGAVMFFVGLAALTFMTASNSMMQ
                     LTTERAMRGRVLALRIAVVMGGTPLGAPLVGWVVDHFGARWALAVGALSGVVATAVGV
                     AYLVRHRDLRVVRKGRRLRLVIRPVPTDAIPARVAAGERVI"
     gene            complement(5801..7975)
                     /locus_tag="VFK00_11600"
     CDS             complement(5801..7975)
                     /locus_tag="VFK00_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HET7593631.1"
                     /translation="MGVILLQGPLARRFRPLLWLGVCFVVIAFVTRLILLLMTGSGVP
                     PNPLDWLYAFGVGLGYDLVTFVYFAWPMVLFLWLVPTRRGRLPGWARWLVYALLLVGL
                     LAICAGLLRWQFHADWKTAWPVVLPFLFALPLAAFTYSSRVGQWILWAFCLLLLFGLL
                     FVGASELTFWNEFGSRFNFIAVDYLIYTREVIGNIEESYPVARWLAMLAVAAIVIVAL
                     TRRSLRTRDDDSSFGGRSLVVLGWLAITIITAFTVNSGMKDRLRNTYVNSLAGNGIYE
                     FFAAFRNASLDFHRFYRTLPDAEAYAIARTMLKTPNATWVGSDPHDITRAIHAAGPER
                     HLNVVLITVESLSAGYLGVYGGKKDLTPNLDRLAAQSLFFKNMYANGTRTVRGLEAVT
                     LSIPPTPGDSLIKEKHNENLFSLADIFNQRGYVSEFVYGGYGYFDNMNYFFGHNGYKD
                     VDRRDIPADATIHSENVWGVADEDLYTLAMGQMDKIHDAGKPFFLHIMTTSNHRPYTW
                     PKGRVVAMPQGKRDGAVKYTDWAIGDFIRRMRAKPYFRDTVFVITADHCADSSGKARI
                     PINRYRIPLLIYSPAHIPPRHIERMISQIDIPPTLLGLLHFSYRSRFFGHDVLDLPPE
                     RDRAFPSTYQNLGYLRGDRLTILSPGSEVEQVNPDPVTGSAVPYPQVDQAEVHQAIAA
                     YQVAYDEFHSGRMRWRASDGAPVQPLPVSAASASAGDNTTGP"
     gene            8193..>8505
                     /locus_tag="VFK00_11605"
     CDS             8193..>8505
                     /locus_tag="VFK00_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593632.1"
                     /translation="MLRCVFILTCSLLPGLLLVSPAGAATEPAENCSLAELGVHGPSE
                     IRAFGGALRSALDQGPTALAKLAQFPLSVTYPDGQQVMVHDAVALRNELNVPALHALE
                     RH"
BASE COUNT         1685 a         2770 c         2668 g         1382 t
ORIGIN      
        1 gggcatcaac ttcgccgcag gtgcgacgat cccgaagcgc cttgtctctg atggcggttt
       61 cggttttgcg gcccacacca cggcgttcga ggacaagctg gggcgcggaa cgcggctcac
      121 catccgctgt accgatgccc gcaagcgcgc ggacgtcgat ctccacttgt cgctctacga
      181 cgaactgcag gcgctcacgt tcgaggcgac ctgcaccaat gcctcgcaag ccgacatcgc
      241 gatccacagc ctggagccgg tgcgcgcggt cgcgaacgaa cacggcgtgc tgcatgcctc
      301 cggtgtgtcg aaatgcctca ccaacggcga gatgtatttc gacgctggca ccgtgcacac
      361 gttcggcgaa agggaaggcg cgatcacctc cgggcacctc aagggtgtca atctcgccaa
      421 cgggccgttc ccggggcaaa gcgaaaccat ccacagttgg tggaatgccg gccttttctg
      481 cggccatgat cgcgaaagca tcaccttggg atacctggag aacacactgt gcctcggcaa
      541 cctgctgatc tcccggcccg cggacgaccg gatttccctc ctcgcagaat cggcattccc
      601 gccgtcggtg acgttgcggc ccggcagctc gatcggctcg aaccggttga tgatcgagat
      661 cgcggacgat ccctaccgcg cgctcgaaag ctacgcggcc gcggtcggca cggccaacga
      721 tgcgcgcacg aaatctatcc tcaacggctg gtgcagctgg ttctacacgc tgtcgcacgt
      781 ctcccagcat gaagtcatgg cgaacaccgc gttcgccgcc gaaaacctga agccgtacgg
      841 gctggagtac atccagatcg atgaaggttt tcagcgctgg cacggcgact gggaaggcaa
      901 cgagcggttc ccgaacggca tgaagtggtt ggcgcagcag atcaggagcc acggcttcaa
      961 ggcggggcta tggatttcgc cctacgtcat cgaggaaccg acgaagctgt tccaggaaca
     1021 ccccgagtgg ctggtcaagg gccgcgacgg cagcccgcag cgcatcggca actggcccgc
     1081 gggcaccgca ccgccggccg acgagaatcc gaagaaatac tgccttgacg tcacccatcc
     1141 cggcggcgcg aagtggctgc acgacctggt cgacaccatc gtgaacgact ggggttacga
     1201 aatgatcaag atcgatttcg tgttctggtc gatactcgca gccgagcggt tccacgatcc
     1261 gacaatgtcg accgccgcgg cctaccgcaa gggcatggag atcatgcgcc gcgcggccgg
     1321 cgacaagtgc cacatcctcg aatgcggacc gggcgcgatc accacgggcc tgatcgacag
     1381 catgcgcatc gaagccgacg tgaactacgg cttcagcgat accgcgtggg aaacctattt
     1441 cacccaccct gccagcagtt cctccgccgc ggccaagcgc tactacttcc acaagcggac
     1501 ctggatcaac gacgcggacc acctgtgcat ggacctgctc accgacaccg agtccgaagc
     1561 ggcagccacg ctgatcgcct tcagtggcgg caacctgttt tccggcgacc ggctgacgca
     1621 actggaaccg cccaagctcg agatcctgaa aaagatcacg ccctcgctgg gccaggcggc
     1681 cgttccggtg gacctgttcg aacatgaaat gcagtacgcc ttcgcgatga aggtggagcg
     1741 gccgttcgcc gagtggaccc tgctcgcggt gttcaatccg gatcgcaagg aaccggttac
     1801 ccgcaaattc cggctcgacc ggctggggct gcgcccggac ggcacgtaca tcgcgttcga
     1861 cttctggaag cagcaactgg cccgcgagct gcgcggcgcc gaaatcgaag tcacggtcga
     1921 acccgggagc gtgaccctgc tcgcgctgca caaacgatcc ggcattcccc agttcgtgtc
     1981 caccgaccgc cacgtgtcgc agggcggcgt ggagatcgag gacgtgcatt gggatggagc
     2041 gacgaaaacg ctttccggga catcgaccgg tccgctgcat tcggcccatg acgttttcgt
     2101 gtacgtgccc gacgcgctcg ggtggacctg ggagggcagc gcgtttttcc gcgaccacgg
     2161 tgcatacacc ctgaagctgg tcgaccgcca cctcatccgc gtgcacgcca acttcgatac
     2221 agccgatcag gtgcgctgga aggcgaggta tggcgagttc ctgaaatgaa agcccggacg
     2281 cgcggcaccg gcggaccctc ctgcccccgg gaaaagacgg acgttgcgga catttcacga
     2341 gggtgacgcg ggcgttcgag ccaccggttc acggaggtct ccacccgccg atcctgtcgg
     2401 atgtctttac aaaatccgca gattgcacgg gcgcacgata tcgcaagctg ctgaataaca
     2461 aaagcattgt gtactggcgc aaatcttgct ccgtatgcgc gaccgccagc ggaatcacgt
     2521 tcgtggcggt cgcggaaacg tacgccggag cgccaaggct tccgcgattg caaaaacaat
     2581 cgcgacaagc cccggcattc ctccgggaga tttgcgcaac agggggaaat catgaagaag
     2641 aaactgccgc tcgcggtagc ggtcctcggc ttggcgggcg ttttttcgag cgcctcggca
     2701 acgatgctga acgacaccta ccaaggctcg aacagccacg gctaccagga cgtgatcggg
     2761 ccgagcacct acgacgtcga tggcatggag gcgagccttt cgggcagcaa actcaccgtc
     2821 accatctaca cgaacttcgc cggcaacgcc gggatcgagt cgcagtggac gaaaaacgcg
     2881 acgggcatcg gctacggcga cctgttcctg tcgaccggct ggagccccaa cggcccggct
     2941 acctacgcca acgacaacgc cggtaacggc aaccactgga cctacgcgtt ttccctggac
     3001 gacgcgctca gcaacacggg tggcaccggc accatctacg tgctggatgc cggcaacggc
     3061 agcaaccaca atcccgaagt cctgctctcc gacgccttca tggattgtgc cggccgctgc
     3121 atctaccgcg acgggcaggc agtggcggtg aacacgtcca tggcgacggc gacgaacaac
     3181 accggcacct ggaccgtcgg caatggcttc ctgagcttca gcttcgacac cgcgggcctc
     3241 gatctcgacc ccagcaattt cggcttccac tggaccatgt attgcggcaa tgacgtgatc
     3301 gaaggcctcg gccaggcgac cgcggttccg gagcccggcc caattggact gttcctgctg
     3361 ggtctcggcg tgatcgggct gggcgccgtg cgccagcgca aggccgcgcg ggacatggcg
     3421 tcggggcagt cgaagtaatc cattgcccca cgggcggcgg atgctctggc aggttggtgc
     3481 tgaccccgga cctggtccgg gggaggccca acaccgtctc gcgcacgatc cgatgtcgca
     3541 cgccgagcgc ttggtgttgg gcttctcccg gaacaaggtt ccgggatcaa cccaacctac
     3601 agttccgcgt tcccacgcgg cttcggattg tttttgtagg ttggtgctga gcgcagcgaa
     3661 gcccaacacc gtttcctgcc cgatacgatg tcgcatgccg agcgcttggt gttgggcttc
     3721 tcccggaaca aagttccggg atcaacccaa cctacaagtt ccgcgttccc acgcggcccc
     3781 gaaatgcttt gtaggttggt gctgagcgca gcgaagccca acaccgtttc ctgcacgata
     3841 cgatgtcgca tgccgggcgc ttggtgttcg cttctcccgg aacaaggttc cgggatcaac
     3901 ccaacctacg cgcagcaaag cccgacgggc gtcgcggcgt cattccacat gcgcgccacc
     3961 attcccgcat caaatcacac gctctcccgc cgctacccgc gcgggtatcg catccgtcgg
     4021 caccgggcgg atgaccagcc gcagccgcct gcccttccgc accacgcgca ggtcgcgatg
     4081 cctgacgaga taggcaaccc ccaccgcggt cgccacgacg cccgagagcg cgccgaccgc
     4141 cagcgcccag cgggcaccga aatggtcgac cacccaaccc accagcggcg caccgagcgg
     4201 ggtgccgccc atgaccacgg caatgcgcaa cgccaacacc cggccgcgca tcgcgcgctc
     4261 ggtggtgagc tgcatcatcg aattgctggc ggtcatgaag gtgagcgcgg caaggccgac
     4321 gaaaaacatg accgctccga acgaaaacgg gctcggcatc accgccgcca cggccagggc
     4381 gacagcgaat gccgcggcgg aaacggccat cagcaccatg ccggggaatg gccgacgggc
     4441 cgaaaacagt gctccgctca tggtgccaac cgccatcgcc gaggtcagca ggccgtactg
     4501 gctggcgtcg ccgtggaata cggtcaccga catggtcgag atgaagatcg aaaagttgaa
     4561 gccgaaggtg cccagcagca acagcatcat caacacggca aacaggtcgg gccgctgcca
     4621 cacgtagccg aagcccgcca acaagctgcc gcgcgtgcgc gggggccgcg cctcggtatg
     4681 gagctcgtgc accctcagaa acgacaacga caccagcacc gcggcgtagg agcccgcgtt
     4741 gatgaggaac agccagccct cgccgacggc ggcaatcagt atgcccgcga tcgccggacc
     4801 gaccatgcgg gcggcattga aggacgtgga gttcaacgca accgcgttgg ccaggtcgtc
     4861 atcggtaacg aggtccgaga cgaatgcctg ccgcgccggc gcgtcgaacg cggtgacgca
     4921 gcccagcagc agcgcgaagc catacacctg ccagagccgc accacgcccg tgacagtgag
     4981 cagacccagg cccagtgcca gcagcccggc ggtgccctgc gtgaagagca gcagcttgcg
     5041 ccgatccagg tgatcggcgg cgaacccgct caacggcagc atgaacaatg gcggcccgaa
     5101 ctgcagcgcc atcaccgtac cgaccgccgt agcgttgtgg tgggtcagca cggtcagcac
     5161 cagccagtcc tgtccgatgc gctgcatcca ggtgcccacg ttggatacca gcgcgcctcc
     5221 ggcccacagg cggtagttat ggctcctgag ggactggaac atgctgctca tcgggcgatc
     5281 ggccacctca ccggaacgtt gcagccgcga agccggctgt ggcccggtgc cagccccgcg
     5341 gcggcaggca gcacgccgag tacgacggaa aaacaggcgt caggaacaac tcgagtaggg
     5401 tgtgacgtca gaaattgttc ctgccagcgc ttcgtggagc ccggtcgttc gcgtcgcctt
     5461 gccgagcgag ccgggatcga tcaactgcgg aagcagcaaa tcaacccgca aagtgtaacc
     5521 cgacccaagt tgtgcgagaa attttcttca cccggctctc gaagaaaatc tgaggttttc
     5581 aaaacccggg ggccggagtc gacttttccc gctcgggttc cgaaccctca tccggcgctg
     5641 cgcaagagaa acatttgcgt ttcatgccac tccccggagg ggaaggcaga gctactggat
     5701 cccggccttc gccggaatga cgagcgtttg ccgggggtcg acactcaaca ggtggctgag
     5761 tcgcgcccgg gtgccgaagg cagcgggggt atcgcgaaac tcaaggtcca gtggtgttgt
     5821 caccggcgga cgccgatgcc gcggagacag gcaacggctg caccggcgcg ccatcactgg
     5881 cgcgccagcg catgcgaccg ctgtggaatt catcgtaggc cacctggtag gccgcgatgg
     5941 cctggtgcac ttcggcctgg tccacctgcg ggtacggcac cgcgctgccg gtgaccggat
     6001 ccgggttgac ctgctccacc tcgctgccgg gcgagaggat ggtcaggcga tcgccgcgca
     6061 ggtatccgag gttctggtag gtcgacggaa atgcgcggtc gcgctccggc ggcaagtcca
     6121 gcacatcatg gccgaagaac cggctgcggt agctgaagtg cagcaggccg agcagggtcg
     6181 gtggaatgtc gatctgcgag atcatgcgct cgatatgccg cggagggatg tgcgccggcg
     6241 agtagatcag caacgggatt cgataccggt tgatcgggat gcgcgccttg ccggaactgt
     6301 cggcgcagtg gtcggcggtg atcacgaaca cggtgtccct gaaatacggc ttggcccgca
     6361 tgcgccggat gaaatcaccg atggcccagt cggtgtactt gacggcgccg tcgcgcttgc
     6421 cttgcggcat ggccaccacc cggcccttcg gccaggtgta gggccggtgg ttggaggtgg
     6481 tcatgatgtg caggaaaaac ggcttgcccg cgtcgtggat cttgtccatc tggcccatcg
     6541 ccagggtgta caagtcctcg tcggccaccc cccagacgtt ctcgctgtgg atcgtcgcat
     6601 ccgcggggat gtcgcgacgg tcgacatcct tgtagccgtt gtgcccgaag aagtaattca
     6661 tgttgtcgaa gtagccgtag ccgccgtaga cgaactccga cacatagccc cgctggttga
     6721 agatgtccgc cagcgagaac aggttttcgt tgtgcttctc cttgatcagc gagtcgcccg
     6781 gcgtcggcgg aatcgacagc gtcacggctt ccagcccgcg cacggtgcgc gtgccgttcg
     6841 catacatgtt cttgaagaac aggctttggg cggccagccg gtcgagattg ggggtcaggt
     6901 ccttcttgcc gccgtacacg ccgaggtagc ccgcggacag gctttcgacg gtgatcagca
     6961 ccacattgag atgccgttcc ggtccggcag catggattgc gcgggtgatg tcgtgcggat
     7021 cgctgccaac ccaggtcgcg ttcggcgttt tcagcatcgt gcgcgcgatc gcgtaggcct
     7081 ccgcgtccgg caaggtccga tagaaccggt ggaaatcgag gctggcattg cggaatgccg
     7141 cgaagaattc gtagatgccg ttgccggcga gcgaattgac gtaggtgttt cgcaggcgat
     7201 ccttcatgcc cgagttgacg gtgaacgccg tgatgatcgt gatcgccagc cagcccagca
     7261 ccaccagcga gcgtccgcca aagctgctgt catcgtcgcg cgtgcgcagg ctccgtcgcg
     7321 tgagtgcaac gatcacgatg gcagcgaccg ccagcatcgc cagccaacgc gccaccggat
     7381 acgactcctc gatgttgccg atcacctcgc gcgtatagat caggtagtcc accgcgatga
     7441 agttgaagcg cgagccgaac tcgttccaga acgtcaattc cgacgcaccc acgaacagca
     7501 acccgaacag cagtagcagg cagaaggccc acaggatcca ttggccgaca cgcgaggagt
     7561 aggtgaacgc cgccaacggc agcgcgaaca ggaatggcag caccaccggc cacgccgtct
     7621 tccagtcggc gtggaactgc cagcgcaaca gtcctgcgca gatggccaac aggcccacca
     7681 gcagcaacgc atagaccagc cagcgcgccc agcccggcag gcgcccgcgc cgcgtcggca
     7741 ccagccacag gaacaacacc atgggccagg cgaagtagac gaacgtcacc aggtcgtagc
     7801 cgagaccgac gccgaaggca tacagccaat ccagcggatt cggcggcacg ccggagccgg
     7861 tcatgagcaa caggatcagg cgggtgacga acgcaatgac gacgaagcac acccccagcc
     7921 acagcaacgg gcggaagcgt cgcgccagcg gcccctgcaa caagattacg cccacgcgtt
     7981 gttgctccga tgtagtaatg ccgcggctgc ccgcatgatt tgtttgaagt ggtggaattc
     8041 gccgccaaca tcgtaaaggg gacggagcta taagcacaca gtaattacct ccgtcctgtt
     8101 ttgagttgcc tcgctactcg gctcggcgta ccgtaaagcc cgtacttgtt gctgtgtgct
     8161 cgattccgct cggcactacc agggaggtcg ccatgctgcg ttgcgttttc atactgacgt
     8221 gctcactgct accggggttg ttgcttgttt cgccggccgg ggcggccacg gaacccgcgg
     8281 aaaattgcag tcttgccgag ctgggcgtgc atgggcccag tgagatccgg gcgttcggcg
     8341 gcgctcttcg tagcgcactg gaccagggcc caacggcact ggccaaactc gcgcaatttc
     8401 ccctgtccgt gacctacccg gacggccagc aggtcatggt gcacgacgca gtcgctttgc
     8461 ggaacgagct gaatgttcct gcgctgcacg cgctcgagcg gcatc
//
LOCUS       DASSNH010000310         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1387823, whole genome shotgun sequence.
ACCESSION   DASSNH010000310 DASSNH010000000
VERSION     DASSNH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387823"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFK00_11610"
     CDS             complement(<1..477)
                     /locus_tag="VFK00_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl carboxypeptidase II"
                     /protein_id="HET7593633.1"
                     /translation="MPGLRTKFLALACCTLVGGIGLSAHADTPASGASSGGDIASNPF
                     FKPSTLPFGTPDFSKIQDSDYVPAIEKGMALQKQEIEKIADNPEPPTFDNTIVAMEKT
                     GAMLDRVMSVFNLYTGADTDPKLQKISEEMAPKLAAHQDAIYLNAKLFDRIQKIYDQ"
     gene            complement(524..874)
                     /locus_tag="VFK00_11615"
     CDS             complement(524..874)
                     /locus_tag="VFK00_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593634.1"
                     /translation="MSQAQRYYLTIPNLGKAHGPDPELSFQGASPQSFATALQEALRQ
                     PILFQRWKARQPDPDEVDQSLAPVDPNATVTAEMHDLHTAVQVVTTLTHSVLRHRLFI
                     LIGPNWQLHDVAAA"
     gene            1049..>3570
                     /locus_tag="VFK00_11620"
     CDS             1049..>3570
                     /locus_tag="VFK00_11620"
                     /inference="COORDINATES: protein motif:HMM:NF026035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593635.1"
                     /translation="MSPTIRKSVLAGCALLAGAGVAACSQSSSPPASGDTSASVPSAS
                     TTPVTPVAPPPPAATSAGAVASAGADVEGTRLLRFPDVCGDRVVFTYAGDLWTASTRG
                     GTATRLTAGPGLEMAARFSPDCRQIAFSGQYGGDSQVYVVPATGGEPKQLTFYPAMGP
                     LPQRWGFDNQVYGWTPDGKAVLFRSWRDSRNISDPRVFTVAVTGGLPSVLPMPQSGTA
                     RFSPDGKQMVYSPKFRDFRDWDRYKGGWAQDLFIYDFASKSAKNITNDPNTDRDPVWI
                     GKEIYFLSDRGPHLNLYRYDTGDGKTTQLTDYKGDDARWASGDAAGQIVYEVTGALHI
                     YDTRNNTDRALTIHVPSDLVAARASERGVKKYIEDYALSPNGKRAAFTARGDVFSVPL
                     EHGITLDLTHTPGAHEREVSWSPDGKRVAYISDQSGEEEVWVRDADGTDPVMLTKGVI
                     GRLYQPLWAPDGKHIAFVDSGNRIHVIGTSANANDQVVAEDPGVSRRDYAWSPGGHYL
                     AYSLTDKDTQLSRLYVYDLGSGKAVELGREYADALAPAFSPDGKYLYFLGDREWSPQF
                     SSVELDYATNRTTQILAYALRKDVDNPFAPRNDSATADDDGAKGASGEGKDHGDHKQP
                     KPASEVNDKIDFDGIEQRLVRAPIDPDNVQWLAVTSKAILYATSGAPFLGRASALPLK
                     VHAWSFKDRKDSDVYTYKPQGGGDGGGGAAPLALSADGSTLLVHDGDDYKAIDLGESK
                     PKPREVKLDGLFMRVDPRAEYAEIFNDVWRRYRDYFYVANMNGYDWDALRAKYQPLLQ
                     YVGDRTDLNYVLGEMIAELSNSHTYIAGGDLGLPDKPHVGLLG"
BASE COUNT          619 a         1156 c         1203 g          592 t
ORIGIN      
        1 ctggtcgtag atcttctgga tccggtcgaa cagcttggcg ttgaggtaga tcgcgtcctg
       61 gtgggccgcg agcttgggcg ccatttcctc cgaaatcttc tgcagcttcg ggtcggtgtc
      121 ggcgccggtg tacaggttga agacgctcat gacccggtcg agcatcgcgc cggtcttctc
      181 catcgcgacg atggtgttgt cgaaggtcgg cggctcgggg ttgtcggcga tcttctcgat
      241 ctcctgcttt tgcagcgcca tgcccttttc tatcgcgggc acgtagtcgg aatcctggat
      301 cttgctgaag tccggcgtgc cgaacggcag ggtcgatggc ttgaagaacg gattggaagc
      361 gatgtcgccg ccggacgacg cgccgctggc gggggtatcg gcgtgcgcgc tcaaaccgat
      421 gccgccgacc agggtgcagc aggcgagtgc gaggaatttg gtgcgtaatc cgggcatgga
      481 gcgactcctt ccattgcaga aaaaaggggg aaccgccgaa ggttcaggcg gcggcgacgt
      541 cgtgcaactg ccagtttggc ccgatcagga tgaacaggcg gtggcgcagc acggaatgcg
      601 tgagggtggt gacgacctgg accgcggtat gcaggtcgtg catctcggcg gtgacggttg
      661 cgttcggatc gaccggtgcc agcgactggt cgacctcgtc ggggtcgggc tgccgcgcct
      721 tccagcgctg gaacaggatc ggctgtcgca gcgcttcctg cagggccgtc gcgaagcttt
      781 gcggcgacgc tccctggaac gacagctcgg gatcgggccc gtgggccttg ccgaggttgg
      841 ggatcgtcag gtagtagcgt tgggcttggc tcaaggtcct ggctccggca gcgctcgagg
      901 cggggaggat cgaccaccaa tgatgccgcg atcgaggtcg gggtgccatc catgccgcaa
      961 gtcatgccgc acatgacttt ccgcagtggc tcgcgggccg gaatcccgct atagcaggcc
     1021 atccacgcca cggcacgagg ggcccgacat gtcacccacc attcgcaagt ccgttctcgc
     1081 gggttgcgcg ctgctggcag gcgcgggagt ggccgcctgt tcgcagtcgt cttcgccccc
     1141 cgcgtcgggc gacacgtcgg catccgtgcc ttccgcgagc accacgccgg taaccccggt
     1201 cgcgccgcca ccgccggccg ccaccagcgc gggtgccgtc gcctccgcgg gtgccgacgt
     1261 cgaaggcacc cgcctgctgc gcttccccga tgtgtgcggc gaccgggtgg tgttcacgta
     1321 cgccggcgat ttgtggacgg catcgacccg aggcggcacc gcgacccggc tgaccgccgg
     1381 gccgggcctc gaaatggccg cgcgcttctc gcccgactgc aggcagatcg ccttctccgg
     1441 ccagtacggt ggcgacagcc aggtgtacgt ggtgcccgca acgggcggcg agccgaagca
     1501 gttgacgttc tatcccgcga tggggccgct gccgcagcgc tggggcttcg acaaccaggt
     1561 gtacggctgg acgccggacg gcaaggccgt gctgttccgc tcgtggcgcg actcgcgcaa
     1621 catctcggat ccgcgcgtct tcaccgtggc cgtcaccggc ggcctgccga gcgtgctgcc
     1681 gatgccgcaa tcgggcaccg cacgcttttc gcccgacggc aagcagatgg tgtactcgcc
     1741 caaattccgc gatttccgcg actgggaccg ttacaagggc ggctgggctc aggatctctt
     1801 catctacgac ttcgcctcga agtcggcgaa gaacatcacc aatgatccca acacggaccg
     1861 cgacccggtg tggatcggca aggaaatcta tttcctgtcc gaccgcggtc cgcacctgaa
     1921 cctgtaccgc tacgacaccg gcgacggcaa gaccacgcaa ctgaccgact acaagggcga
     1981 tgacgcgcgc tgggccagcg gcgacgccgc cggccagatc gtgtacgaag tgaccggtgc
     2041 gctgcacatc tacgacaccc gcaacaatac ggaccgtgcg ctgacgatcc acgtgccgag
     2101 cgacctggtc gccgcgcggg catccgagcg cggcgtcaag aagtacatcg aggactacgc
     2161 gctcagcccc aacggcaagc gcgcggcgtt cacggcgcgc ggcgacgtct tcagcgtgcc
     2221 gctggaacac ggcatcacgc tcgacctcac ccacacgccg ggcgcgcacg aacgcgaagt
     2281 gtcgtggtcg cccgacggca agcgcgtcgc ctacatctcc gaccagagcg gcgaggaaga
     2341 agtgtgggta cgggatgccg atggcaccga tccggtgatg ctgaccaaag gcgtgatcgg
     2401 acggctttac cagccgctgt gggcgccgga cggcaagcac atcgcgttcg tggattcggg
     2461 caaccgcatc catgtgatcg gcacctcggc caatgcaaac gaccaggtgg tcgccgagga
     2521 tcccggcgtg tcgcggcgcg actacgcatg gtcgccgggc gggcattacc tcgcatattc
     2581 gctgaccgac aaggacacgc agttgtcgcg gctgtacgtg tacgaccttg gttccggcaa
     2641 ggccgtcgaa ctcggccgcg aatatgccga cgcgctggca ccggcgttct cgcccgacgg
     2701 caagtacctc tacttcctcg gcgatcgcga atggtcgcca cagttctcca gcgtggaact
     2761 ggattacgcg accaaccgca cgacgcagat cctcgcgtat gcgttgcgca aggacgtcga
     2821 caatccgttc gcgccgcgca acgacagcgc gacggccgac gacgatggcg caaagggtgc
     2881 atcgggggaa ggcaaggacc acggcgatca caagcagccg aagcctgctt ccgaggtcaa
     2941 cgacaagatc gacttcgacg gcatcgagca gcgcctggtg cgcgcgccga tcgatcccga
     3001 caatgtccag tggctggcgg tcacgtccaa ggcgatcctg tacgcgacca gcggtgcacc
     3061 gttcctcggc cgcgcaagcg cgctgccgct gaaggtgcac gcgtggtcgt tcaaggaccg
     3121 caaggacagc gacgtctaca catacaagcc gcagggcggc ggtgacggcg gcggcggcgc
     3181 ggcgccgctg gcattgtcgg cggacggctc gacgctgttg gtgcacgacg gtgacgatta
     3241 caaggccatc gacctcggcg agtcgaaacc caaacccagg gaggtgaaac tggacggcct
     3301 gttcatgcgg gtcgatccca gggccgagta cgccgagatc ttcaacgacg tgtggcggcg
     3361 ctatcgcgat tatttctatg tcgccaacat gaatggctac gactgggatg cgctgcgcgc
     3421 gaagtaccag ccgctcctgc aatacgtcgg cgatcgcacc gacctcaatt acgtgctggg
     3481 cgagatgatc gccgagcttt ccaactcgca cacctacatt gcgggcggcg atctcggctt
     3541 gccggacaag ccgcacgtcg gcctgctggg
//
LOCUS       DASSNH010000311        14791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_256848, whole genome shotgun sequence.
ACCESSION   DASSNH010000311 DASSNH010000000
VERSION     DASSNH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 14791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14791
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256848"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFK00_11625"
     CDS             <1..1120
                     /locus_tag="VFK00_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7593636.1"
                     /translation="EVQPGETLSEIFQEQGLSSADLASVMASGKDTGALKTLHPGDQL
                     EFLIGAKGRLDGFRYSPDAGSQVTLTAQADGSMRAQTTTLPVERRMHFAHGEVQGSLF
                     AAGEKAGLSETMVLKLADVFKYDIDFIKDLKQGDRFTVVYDDVYRNGKYAHGGDIIAA
                     EFVNEGHRYTAYRFKRGDGSIAYYNQEGRPLRKGLLRTPVSFTRISSRFGMRDHPILG
                     YTRMHKGVDYAAPTGTPIHAAGDGVIVRRGWVHGYGNFIAIKDTPKYTTAYGHMSRFA
                     KGLHVGSHVTQGQVIGYVGQTGMATGPHLHYEVRVNGKPENPLTVTMPKPQPLSGKLM
                     AEFKAQSAPMIARIQMIDNATQQLARVDNDHNDVATTN"
     gene            1137..2297
                     /locus_tag="VFK00_11630"
     CDS             1137..2297
                     /locus_tag="VFK00_11630"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET7593637.1"
                     /translation="MPPLFLGLISGTSADGIDAALVRFAPKLEVLAARTFPYPDDLQA
                     RILALARSRAEITLDDLGHLDVEIGERFAAAASGLLREAGIAGGAVAAIGSHGQTVCH
                     RPHGPLPFTLQLGDPNVIAERTGIVTVADFRRADVAAGGQGAPLLPALHAAVLADPAR
                     ARAVLNLGGIANLTLLVPGKPVIGFDSGPANCLLDGWSMRARGVPRDEGGAWAAGGRV
                     DDALVARLLEDAYFAAPPPKSTGREHFNLDWLDARLTGDIDAVDVQATLLQLSARSIA
                     NALRQTSPDVREVYACGGGVHNDALMAALRAALPDATVATTAALGLDPDFVEAAGFAW
                     LARARLEGMPGNLPSVTGAGGPRVLGGIYAPAVRHGSAHDGTGAQGGHRPAA"
     gene            complement(2343..3611)
                     /locus_tag="VFK00_11635"
     CDS             complement(2343..3611)
                     /locus_tag="VFK00_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7593638.1"
                     /translation="MATKAAADDPVPDVRGRNWRILSIGLLGFSSGLPLALSGATLQA
                     WFTTAGMSLKDIGWITVIGQAYVFKFLWAPLLDRVRLPWLGRRRGWVLLMQAICGAAL
                     VAMSFETPQGAAVLLAFFAVLLAFASATQDIAYDAHRTDLLRPAERGWGIGFMQGGYR
                     IAMLVSGAFALILADHAGWAFTYRVMGVLMWGAMIVTVASPDAPDERPTRTFVSAVVE
                     PFADFFRRYGKLAIAWLVLMVLYKLCDAFALSLSTTFLLRVPQFSLTQIGTVSKTFGL
                     VAGLLGAVSGGWVVTRMRLFPALLVLGTCQAVVNLGYIWLVHSGPDVPAMATVVSLEY
                     FFSGLGSTAFVVLVTGLCNVRFSATQFALLSALAAVGRVFLGPVAAWLVPQVGWSNFF
                     AITALSAAPGLLLVIGLRRAIHDVEGARPT"
     gene            complement(3601..4401)
                     /locus_tag="VFK00_11640"
     CDS             complement(3601..4401)
                     /locus_tag="VFK00_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7593639.1"
                     /translation="MRIISFNANGIRSAARKGFFDWLATQNADVTCVQETKCQEHQLT
                     DGMFRPDQHHCFYRDAVTKKGYSGVAIYAKHEPDEVLTRIGWQPLDDEGRYIEARFGN
                     LSVVSLYVPSGTSGELRQQFKFQAMKKLRTIFDGWLTSGRDYVLCGDWNIVRSERDIR
                     NWKSNQKNSGCLPQERAWLNALIADPHGDGAPAPEHGWLDSFRVVKPDAEEYTWWSNR
                     GAARANNVGWRIDYQITTPGIGKRARACAIAREPRFSDHAPYLVEYGD"
     gene            4539..5192
                     /gene="pyrE"
                     /locus_tag="VFK00_11645"
     CDS             4539..5192
                     /gene="pyrE"
                     /locus_tag="VFK00_11645"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7593640.1"
                     /translation="MLQPHQQSFLDTALACGVLRFGEFTLKSGRLSPYFFNMGKIDSG
                     GALATVGDAYAATAQASGIAFDMLFGPAYKGIPLAAATAIALSRRHDLDIPWAYNRKE
                     AKDHGEGGTIVGAPLRGRVLIVDDVITAGTAVREALGMIRAAGATVAGVLIALDRQER
                     GQGEHSAAQELASGEGAPVVAIVRLADLLDYAGTRPELAEQRDRLSAYRDRYGVAAA"
     gene            5267..5935
                     /locus_tag="VFK00_11650"
     CDS             5267..5935
                     /locus_tag="VFK00_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7593641.1"
                     /translation="MHRFAPILFAAALVGFGLPTAAIAANNTTPDTHYRWKDASGVVH
                     FGDTIPASALAGGYDIVNSQGMVVRHVSRELTPAERRAAAAAVARETAARRAAQQRKV
                     ADAQLLAAYPTEQALDESQQAQLHQIQTDIATLETNLRSQEDSLTELLAHAADIEHSG
                     KPVPPYVNKRIADQRRTVNDERSALAQRHADYAQAKVQFAARLEHYRTLRAKFQVEGG
                     PQQQ"
     gene            complement(6088..6360)
                     /locus_tag="VFK00_11655"
     CDS             complement(6088..6360)
                     /locus_tag="VFK00_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET7593642.1"
                     /translation="MTNQTPAEQELAKLIVENLNLENVAPESIDPEAPLFGGNLGLDS
                     IDALELALAVSKRYGIQLRSDNPDNRRVFGSLRALSEHIESRRTAA"
     gene            6510..7436
                     /locus_tag="VFK00_11660"
     CDS             6510..7436
                     /locus_tag="VFK00_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine-dependent deoxygenase"
                     /protein_id="HET7593643.1"
                     /translation="MAYHAEPASALLEKPDTLAVFGFGSEDAAAGDPRHVRIPLPVLS
                     RDPLHECWSVAGAVTHGCGDGLRWSTGGGRRFVAVEARETPAAGIEAISERAYRSLLD
                     HVGGCPERHLLRIWNYLGAINEGDDDHERYRLFCNGRARGLAAHGITTYPAATAIGHH
                     GPRGLLQVYALCASEPGNALENPRQVSAWRYPREYGPTAPSFARAMALPNGGLAISGT
                     AAVIGHASHHHDDVVAQAHEAFANLETLLQRAGMRPFDASSPLKVYIRNPEEAPAVEA
                     ALARHLDPSVPRLLLQGDICRRELLVEIDGWR"
     gene            complement(7454..8815)
                     /gene="glmU"
                     /locus_tag="VFK00_11665"
     CDS             complement(7454..8815)
                     /gene="glmU"
                     /locus_tag="VFK00_11665"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7593644.1"
                     /translation="MADPLHVIVLAAGEGRRMKSLLPKVLMPLAGKSLLAHVLDTARA
                     LRPEGLHVVYGHRGEEVRTVFARDADIHWVHQSEQRGTGHAVRLALHDIPDPARILVL
                     YGDVPLLRAETLTELLHTHAELAVLAAELDDPTGYGRVLRDGLGRVRAIVEEKDADEQ
                     QRATHVVNTGVTFAASPQLRRWVDQLSADNQQGELYLTDIFAQAADEGAHADVVMTAD
                     PVEAFGANDAAQLAELAAYLRRRNARRLMLAGVRIADPARFDLRGHFSHGRDVEIDVD
                     VILEDGVTFGDAVKIGPFCRLRNVNLAAGTEVMAHCDLEGVTTYGACRIGPFARLRPG
                     TELAAGVHVGNFVETKKARLGEGSKANHLTYLGDTRVGAHVNIGAGTITCNYDGVHKH
                     LTEIGDGAFVGSNTSLVAPVRVGSNATIGAGSTITADTPPDKLTLARAKQATLKGWRR
                     PDK"
     gene            complement(8913..9335)
                     /locus_tag="VFK00_11670"
     CDS             complement(8913..9335)
                     /locus_tag="VFK00_11670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7593645.1"
                     /translation="MTTIRCDIVSAEAEIFHGEATLVIASGEAGELGIAPRHEPLLTR
                     LKPGQVEVIGEDGKRQSYYVSGGILEVQPNVVTVLADAALRADEIDEAAVKRAKEEAE
                     RVLENRGDAKTVEQAQQQLTQAVAQLQALERLRRNLQH"
     gene            complement(9423..10865)
                     /gene="atpD"
                     /locus_tag="VFK00_11675"
     CDS             complement(9423..10865)
                     /gene="atpD"
                     /locus_tag="VFK00_11675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7593646.1"
                     /translation="MSQGKIIQIIGAVVDVEFPRDAVPKVYDALKVQGIQVTLEVQQQ
                     LGDGVVRTIALGSTDGLKRNLVVDNTGAAIKVPVGKATLGRIMNVLGEPIDERGPVNA
                     EDQWEIHRAAPAYEDQAAANELLETGIKVIDLMCPFARGGKVGLFGGAGVGKTVTMLE
                     LINNIATEHSGLSVFAGVGERTREGNDFYHEMIEAGVVKPDNFPESKVAMVYGQMNEP
                     PGNRLRVALTGLTMAEYFRDEKDASGKGRDVLMFIDNIYRYTLAGTEVSALLGRMPSA
                     VGYQPTLADEMGRLQERITSTKTGSITSIQAVYVPADDYTDPSPATTFAHLDSTVALS
                     RDIASLGIYPAVDPLASTSRQLDPNVIGQEHYDVARKVQGTLQRYKELKDIIAILGMD
                     ELSEADKQAVYRARKCERFFSQPFHVAEVFTGSPGKYVPLKETIRAFKMIVDGEVDHL
                     PEQAFYMVGGIEDAIQKGEQMNAAGEKKAA"
     gene            complement(11015..11935)
                     /gene="atpG"
                     /locus_tag="VFK00_11680"
     CDS             complement(11015..11935)
                     /gene="atpG"
                     /locus_tag="VFK00_11680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010371995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET7593647.1"
                     /translation="MAGAREIRSKIKSVANTRKVTSALQMVSASKIRRAQELMRTSRP
                     YSLHMRNVVAHIAQATPDYQHPALREREVKRVCYLVVSTDRGLCGGLNANLFRKVLAD
                     IHGWHQKGVGVEVIAIGQKAAQFFRRIQVDLVGTVTHLGDRPRLEQLVGVMKIADDAF
                     MQGKIDRVYLCYNQFVNTMTQRATVDVLLPLPAVAEEIKTAAGDELHPASSAARIERA
                     TTWDYLYEPEAEPVIRHVLNRYFEAMVYHATLENLASEHAARMVAMKAASDNATKAID
                     TLTLIYNKARQAAITQEISEIVGGAAAVGG"
     gene            complement(12006..13553)
                     /gene="atpA"
                     /locus_tag="VFK00_11685"
     CDS             complement(12006..13553)
                     /gene="atpA"
                     /locus_tag="VFK00_11685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7593648.1"
                     /translation="MATTLNPSEISELIRARIDQFKLSAETRNEGTLTCVSDGIVRIH
                     GLNDAMAGEMIELPGDTFALALNLERDSVGAVVLGQYEHLSEGDTAKTTGRILEVPVG
                     PELLGRVVDALGNPIDGKGPVNTHATSPVEKVAPGVIWRQSVSQPVQTGYKSVDSMIP
                     IGRGQRELVIGDRQTGKSALCIDTIINQKGTGVKCIYVAIGQKQSSIANVVRKLEEHD
                     ALAHTIIVAASASDSATMQYIAPYAGCAMGEYFRDRGEDALIIYDDLTKQAWAYRQIS
                     LLLRRPPGREAYPGDVFYLHSRLLERAARVNAEYVEKETNGEVKGKTGSLTALPIIET
                     QAGDVSAFVPTNVISITDGQIFLETDLFNAGIRPAVNAGISVSRVGGAAQTKIVKKLS
                     GGVKLALAQYRELAAFAQFASDLDAATRAQLERGQRVTELMKQKQYSPLTIAQLAVSL
                     YAAEKGYLDDLPIEKILPFEHALHQYMEANHGDLMKKIVESGDWNADIEGTFKSALDE
                     FKKTGSW"
     gene            complement(13716..14249)
                     /locus_tag="VFK00_11690"
     CDS             complement(13716..14249)
                     /locus_tag="VFK00_11690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET7593649.1"
                     /translation="MAQQLTIARPYARAAFEYAKAHAAENEWAGKLAFAAEASVEPRV
                     AALFGDPRVEPRALASLFRREGEASDSAFAHFLELLADNRRLRELPEIAALFEQMKRE
                     AEHVLKVRVRSAVPLDDAQAGQLVEALKRRYRSDIDLVRTIDPGVLGGAVIDVGETVI
                     DGSLRSRLAKLETTLMQ"
     gene            complement(14254..14724)
                     /locus_tag="VFK00_11695"
     CDS             complement(14254..14724)
                     /locus_tag="VFK00_11695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7593650.1"
                     /translation="MEINATLIGEIIAFAIMITLTLKFVWPPLIRAIEERQRRIEEGL
                     EAADRARRELADADTHAADEIRKARAEAASIIERANQQAGQIVDKARADALLEAARQK
                     AAAQADIENMAHRARAELREQVATLAVAGAEKILGREVNADTHKALLDELVAGI"
BASE COUNT         2351 a         4974 c         4945 g         2521 t
ORIGIN      
        1 ggaggtccag cccggcgaaa ccttgtcgga gattttccag gagcagggac tgtcttccgc
       61 cgacctcgcc agcgtcatgg ccagcggcaa ggataccggc gcgctcaaga cccttcaccc
      121 gggcgaccag ctcgagttcc tcatcggcgc caagggacgg ctggacggat tccgctacag
      181 ccccgatgcc ggcagccagg tcaccttgac ggcgcaggcc gacggcagca tgcgcgcgca
      241 gaccaccacc ttgccggtcg agcggcggat gcatttcgcg cacggcgagg tgcagggctc
      301 gctgttcgcg gccggcgaaa aggcagggct ttcggaaacg atggtcctga agctcgccga
      361 cgtgtttaag tacgacatcg acttcatcaa ggacctcaag cagggtgacc gcttcaccgt
      421 cgtgtacgac gatgtctatc gcaacggcaa gtacgcgcac ggtggcgaca tcatcgcggc
      481 ggaattcgtc aacgaaggac accgctacac cgcttaccgc ttcaagcgtg gcgacggcag
      541 catcgcctac tacaaccagg aagggcggcc gctgcgcaag ggattgttgc gcacgccggt
      601 gtcgttcacc cgcatttcct cgcgcttcgg catgcgagac cacccgatcc tgggctacac
      661 ccgcatgcac aagggcgtgg actatgcagc gcccaccggc acgccgatcc atgccgccgg
      721 cgacggcgtg atcgtacggc gcggctgggt gcacggctac ggcaacttca tcgcgatcaa
      781 ggacacgccg aagtacacca cggcctacgg ccacatgtcg cgcttcgcca agggcctgca
      841 cgtcggctcg cacgtcaccc agggccaggt gatcggctac gtgggccaga ccggcatggc
      901 gaccggcccg cacctgcact acgaagtgcg cgtcaacggc aagcccgaga atcccctgac
      961 ggtaacgatg cccaagccgc agccgctctc gggcaagctg atggccgaat tcaaggcgca
     1021 gagcgcgccg atgatcgcgc gcatccagat gatcgacaac gccacgcagc agctggcgcg
     1081 ggtcgacaac gaccacaacg acgtcgccac gaccaactga gggcggcgtg gccggcgtgc
     1141 cacccctctt cctcggcctg atttccggca ccagtgcgga cggcatcgac gccgcgctgg
     1201 tgcgattcgc gccgaagctg gaagtcctcg ccgcacgcac gtttccctat cccgacgacc
     1261 tgcaagcgcg gatcctggcg ctggcccgca gccgcgccga gatcacgctc gatgatctcg
     1321 ggcatctcga cgtggaaatc ggcgagcggt tcgcggcggc cgcatccggc ttgctgcgcg
     1381 aagcgggcat cgcgggtggc gccgtcgcgg ccatcggctc gcacggccag accgtgtgcc
     1441 accggccgca cggaccgttg cctttcacgc tgcagctcgg cgatccgaac gtgatcgcgg
     1501 aacgcaccgg catcgtgacc gttgcggatt tccgccgcgc cgatgtcgcg gcaggcgggc
     1561 agggcgcgcc gctgctgccg gcgctgcacg cagccgtgct ggccgaccca gccagggcgc
     1621 gtgcagtcct gaacctgggc ggcatcgcca acctgacttt gctcgttccg ggcaagccgg
     1681 tgatcggctt cgacagcggt cccgcgaatt gcctgctcga cgggtggtcg atgcgggcgc
     1741 gcggcgtccc ccgcgacgaa ggtggcgcgt gggcggctgg cgggcgtgtg gacgacgcgt
     1801 tggtggcgcg cctgctggag gatgcctact tcgcggcgcc gccaccgaag agcaccggac
     1861 gcgaacactt caatctcgac tggctggatg cgcgcctcac cggggacatc gatgcggtgg
     1921 acgtgcaggc cacgctgctg caactgtccg cacgcagcat cgcgaacgcg ttgcgccaga
     1981 cttcgccgga tgtgcgggag gtgtatgcct gtggcggtgg agtccacaac gacgcgttga
     2041 tggcggcgct gcgggcggcg cttccggatg cgaccgtcgc caccaccgcc gctctcggcc
     2101 tcgatcccga tttcgtcgag gccgcgggtt ttgcctggct tgcgcgcgcg cgtctggaag
     2161 gtatgcccgg caatctgccc agcgtcaccg gcgcaggggg gccgcgcgtg ctcggcggca
     2221 tatacgcgcc ggccgttcgc cacggcagcg cccacgacgg aacgggcgca cagggcggac
     2281 atcgtccggc ggcctgatgc gcagcacgtc ccgaggacag ccgcgaacac atccgcgagc
     2341 agctaagtcg gccgcgcgcc ttcgacgtcg tggatggccc ggcgcaaacc gatcaccagc
     2401 aacaggcccg gggccgccga aagggcagtg atcgcgaaaa agttcgacca gccgacctgc
     2461 ggaaccagcc aggccgccac cgggccgagg aatacgcgtc ccaccgcagc gagtgccgac
     2521 aacaacgcga attgcgtggc cgaaaaccgc acgttgcaca ggcctgtcac cagcaccacg
     2581 aacgcggtgc tgcccagtcc gctgaagaaa tattccagcg acaccaccgt tgccatcgca
     2641 ggtacatcgg gcccgctgtg gacgagccag atgtagccca ggttcacgac ggcctggcac
     2701 gtgcccagca ccagcaatgc cggaaacagc cgcatcctgg tcactaccca gccgccgctg
     2761 accgcgccca gcaggccggc caccaggccg aaggtcttgc tgaccgtccc gatctgggtc
     2821 agcgagaatt gcggcacccg caacaggaac gtggtggaca gcgacaaggc gaaggcgtcg
     2881 cagagcttgt agagcaccat caacaccagc caggcgatcg cgagcttgcc gtagcgtcgg
     2941 aagaaatcgg cgaatggttc gaccaccgcc gagacgaacg tgcgtgtggg ccgttcgtcg
     3001 ggagcgtcgg gagaggccac cgtcacgatc atcgcgcccc acatcaatac gcccatcacg
     3061 cggtaggtga aggcccagcc ggcgtggtcg gcgaggatca acgcgaaggc gccggaaacc
     3121 aacatcgcga tccggtagcc gccctgcatg aaaccgatgc cccagcctcg ctcggcggga
     3181 cgcaacaggt cggtgcggtg ggcgtcgtag gcgatgtcct gggtggccga cgcgaacgcc
     3241 agcagtaccg cgaaaaacgc cagcagtacc gcggcgccct gcggggtttc gaaactcatc
     3301 gcaaccaggg ccgcgccgca gatcgcctgc atcagcagga cccagccacg gcggcgtccg
     3361 agccacggca ggcgcacgcg gtcgagcaac ggcgcccaca ggaacttgaa cacgtaggct
     3421 tggccgatca ccgtgatcca gccgatgtct ttcaggctca tgcccgcggt ggtgaaccac
     3481 gcctgcagcg tggccccgga cagggccagc ggcagaccgg acgagaaccc cagcaaaccg
     3541 atgctgagga tgcgccagtt tcgtccccgc acgtccggca ccggatcgtc ggccgcggct
     3601 ttagtcgcca tactcgacca ggtatggcgc gtggtcggaa aaccgcggct cacgcgcgat
     3661 cgcacaggcg cgtgcacgtt tgccgatgcc gggcgtcgtg atctggtagt cgatccgcca
     3721 accgacgttg ttggcgcggg cggcgccgcg gttggaccac caggtgtatt cctcggcgtc
     3781 cggcttgacc acgcggaagc tgtccagcca gccgtgttcg ggcgcgggcg caccgtcgcc
     3841 gtggggatcg gcgatcaggg cgttcaacca cgcgcgctct tgtggcaggc agccggaatt
     3901 cttctggttg gatttccagt tgcggatgtc gcgctcgctg cgcacgatgt tccagtctcc
     3961 gcacagcacg tagtcgcggc cgctggtgag ccagccatcg aagatcgtgc gcagtttctt
     4021 catcgcctgg aacttgaact gttgccgcaa ctcgccagaa gtccccgacg gcacgtacag
     4081 ggacactacg ctcaggttcc cgaatcgcgc ttcgatgtag cgcccctcgt catcgagcgg
     4141 ctgccagccg atccgggtta gcacctcgtc gggttcgtgc ttcgcgtaga tcgcgacgcc
     4201 actgtagccc ttcttcgtca ccgcatcgcg gtagaagcaa tgatgctggt ccgggcggaa
     4261 catgccatcg gtgagctggt gctcctggca cttggtttcc tggacgcagg tgacgtcggc
     4321 gttttgcgtc gccagccaat cgaagaagcc cttgcgggcg gccgaacgga tgccattggc
     4381 gttgaaggaa atgattcgca cgggaccggg gaccgggtac tggggaccgg tgaagcgtaa
     4441 agcaaaagca cacaccttgc cgacccattg ggtcgcggtt ttctgacatg gtgtggcctc
     4501 gcgtatcatt ccggtccccg gttcccggtc cacggccgat gctccaaccc caccaacaat
     4561 ccttcctcga caccgcgctc gcgtgcggcg tgctgcggtt cggcgagttc accctgaagt
     4621 ccggccgcct gagcccgtat ttcttcaaca tgggcaagat cgacagcggc ggcgcgttgg
     4681 cgacggtggg cgacgcctac gcggcgaccg cgcaagccag cgggatcgcg ttcgacatgc
     4741 tgttcggccc cgcctacaag ggcataccgc tcgcggcggc gaccgcgatc gcgctgtcac
     4801 ggcggcacga cctcgacatc ccgtgggcgt acaaccggaa ggaggccaag gaccatggcg
     4861 aaggtggcac gatcgtgggc gcgccgctcc gtggacgggt tttgatcgtg gacgacgtga
     4921 tcactgcggg gaccgcggtc cgcgaggcgc tggggatgat ccgcgcggcc ggcgccacgg
     4981 tggccggcgt cctgatcgcc ctggaccggc aggaacgcgg gcagggcgag cattccgcgg
     5041 cccaggaact cgcgtccggg gagggcgcgc cggtggtcgc gatcgtccgg ctcgcagacc
     5101 tgctggacta tgccggcacg cggccggaac tggccgagca gcgcgaccgg ctctccgcct
     5161 accgtgatcg ctacggcgtg gcggctgcct gaagcggcgg cctgggccgc aggtccggat
     5221 cttgcatgct gccggtccta tactgggccg gcttccggag acctcgatgc accgcttcgc
     5281 cccgatcctg ttcgctgcag ccctggtcgg cttcgggctg ccgaccgcgg ccattgcggc
     5341 gaacaacacc acgcctgaca cccactaccg ctggaaggat gcttcaggcg tggtgcactt
     5401 cggcgacacc atccccgctt cggccctggc cggcggctac gacatcgtca acagccaggg
     5461 catggtggta cgccacgtgt cccgcgaact cacccctgcc gagcggcgtg cagcggccgc
     5521 ggccgtggcg cgggagacgg ccgcccgccg cgcagcccag cagcgcaagg tggcggatgc
     5581 ccaactgctg gcagcctatc cgaccgagca ggccctcgac gagtcgcagc aggcacaatt
     5641 gcaccagatc cagacggaca tcgcgacgct ggagaccaac ctgcgcagcc aggaagattc
     5701 gctgaccgag ctgctcgcgc acgcggcgga catcgagcac tccggcaagc cggtcccgcc
     5761 gtacgtcaac aagcggattg ccgaccagcg ccggaccgtc aatgacgaac gcagcgcgct
     5821 ggcgcagcgc cacgccgatt acgcgcaagc caaggtgcag ttcgccgcgc ggctggaaca
     5881 ctatcgcacg ctacgcgcga agttccaagt ggagggcggt ccgcagcagc aataggaagg
     5941 gcggttcaac gcggctgccg atgatccacc cggagcggct gttgtcgaaa cccgcccgcg
     6001 aatgggcggc gtcctggccc ggcggagagc ctcagtccac aagtatccgg cgccaggtgg
     6061 cgcggcgatc atggatgatc gcaagaatca tgccgcggtt cgcctggact cgatgtgttc
     6121 ggagagcgcc cgcagcgacc cgaacacgcg gcggttgtcc ggattgtccg aacgcaactg
     6181 gatgccgtag cgtttcgaga ccgcgagcgc gagttccagg gcatcgatcg aatcgaggcc
     6241 caggttgccg ccgaacagcg gcgcttccgg atcgatgctt tcgggcgcaa cgttttcgag
     6301 attgaggttc tcgacgatca gcttcgccag ctcctgctcc gcaggggtct gattggtcat
     6361 gctccgcggc tccacaatct caccggatca gccgcgtagt gtagcattgc aggtttggca
     6421 tcacgacgac cgagtacatg cgcccctccc cacgcgaaca cgacatggat gacgttgcaa
     6481 ggagcgacgg ggcccggcct tccttccgcg tggcctacca cgctgaaccc gcttcggcgc
     6541 tgctggagaa gccggacacg ctcgccgtgt tcggtttcgg aagcgaggat gcggcggcgg
     6601 gcgacccgcg ccacgtgcgc atcccgctgc cggtgctgtc gcgcgatccg ctgcatgaat
     6661 gctggtccgt cgctggtgcg gtgacccacg gctgcggcga cgggttacgc tggtcgacag
     6721 gcggcggccg gcgcttcgtc gcggtggagg cccgggagac gccggcggcg gggatcgagg
     6781 ctatcagcga gcgcgcctat cgatcgctgc tggaccacgt cggaggttgt ccggagcggc
     6841 acctgctgcg gatctggaat taccttggcg cgatcaacga gggcgacgac gaccacgagc
     6901 gctaccggct gttctgcaac ggccgcgcgc gcggcctggc cgcgcatggc atcaccacct
     6961 atccggccgc gaccgcgatc gggcaccatg gaccgcgggg gctgctgcag gtctacgcgt
     7021 tgtgcgccag cgagcccggc aacgcgcttg aaaatccacg ccaagtgagc gcctggcgct
     7081 atcctcggga gtatgggccg accgccccga gcttcgcgcg tgcgatggcc ctgccgaacg
     7141 gcgggcttgc gatttcggga acggccgcgg tgatcggcca cgcctcgcac caccacgacg
     7201 acgtcgtcgc ccaggcccac gaagccttcg ccaatctcga aaccctgctg caacgggccg
     7261 gcatgcgccc gttcgacgca agctcgccgc tcaaggtata catccgcaac ccggaagagg
     7321 cgccggcggt cgaagccgcg ctggcgcggc acctggatcc gtccgtcccg aggctgttgc
     7381 tgcaaggcga catctgccgt cgcgaactgc tggtcgaaat cgacggctgg cggtagggtc
     7441 gataaaaccg gttttacttg tccggccgac gccagccctt gagcgtggct tgcttggcgc
     7501 gcgcaagggt gagcttgtcg ggcggggtat ccgcggtgat ggtcgagccg gcgccgatcg
     7561 tcgcgttgct gccgacgcgc accggcgcga ccagcgaggt gttggagccg acgaacgctc
     7621 cgtcgccgat ctcggtgagg tgcttgtgca cgccatcgta gttgcaggtg atcgttcccg
     7681 cgccgatgtt gacatgggcg ccgacccggg tgtcgccgag ataggtgagg tggttggcct
     7741 tgctgccttc ccccaggcgc gccttcttgg tttcgacgaa gttgccgacg tggacaccgg
     7801 cggccagttc ggtgccgggc cgcagccgcg cgaacggacc gatccggcag gcgccgtagg
     7861 tggtgacgcc ctcgaggtcg caatgcgcca tcacctcggt gccggccgcg aggttcacgt
     7921 tgcgcagccg gcagaacggc ccgatcttca cggcgtcgcc gaacgtcacc ccgtcctcga
     7981 ggatcacgtc gacgtcgatc tcgacgtcgc gcccgtggct gaaatggccg cgcaggtcga
     8041 aacgcgcggg atcggcgatg cggaccccgg ccagcatcaa gcgcctggcg ttgcgccgcc
     8101 gcaggtaggc cgcgagctcg gcgagctgcg ccgcatcgtt cgcaccgaat gcctcgaccg
     8161 ggtccgcggt catcaccacg tccgcgtgcg cgccctcgtc cgcggcctgc gcaaagatgt
     8221 cggtcaggta caactcgccc tgctggttgt cggcggagag ctggtccacc catcgccgca
     8281 actgcggcga tgccgcgaac gtgaccccgg tgttgaccac gtgcgttgcg cgctgctgtt
     8341 cgtcggcgtc cttttcctcg acgatcgcgc ggacgcggcc caggccatcc ctgagcaccc
     8401 ggccatatcc cgtcggatca tcgagttcgg ccgccagcac cgcgagttcc gcgtgggtat
     8461 gcagcagttc ggtcagcgtt tccgcacgca gcagcggcac gtccccgtac agcaccagga
     8521 ttcgtgcggg atccggaatg tcgtgcagcg ccagccgcac ggcgtggccg gtgccgcgct
     8581 gctcggactg gtgcacccaa tggatgtcgg catcgcgcgc gaacaccgtg cgcacttctt
     8641 cgccgcggtg cccgtacacc acatggagcc cttcgggccg cagtgcgcgc gcggtgtcca
     8701 ggacgtgcgc gagcagggac ttgccggcga gcggcatcag caccttcggc aacagcgact
     8761 tcatgcgcct gccctcgccc gcggcgagga cgatcacgtg cagcgggtcg gccatcacga
     8821 tgctccgtca gggccgcaca agatagcgca acggcgccgt cccggcacct gtcgaaaggc
     8881 acgccgcggc cggcgtacag gcacgacggg aatcagtgct gcaggttgcg gcgcaggcgt
     8941 tccaacgcct gcagctgcgc gacggcctgg gtaagctgct gctgcgcctg ctcgaccgtc
     9001 ttggcgtcgc cacggttctc gagcacgcgt tcagcctctt ccttggcgcg cttgaccgcg
     9061 gcctcgtcga tctcgtcggc gcgcagcgca gcgtcggcga gcaccgtgac gacgttgggc
     9121 tgcacctcga ggatgccgcc cgagacgtag tacgactggc gcttgccgtc ctctccgatg
     9181 acctcgacct ggccgggctt gaggcgcgtc agcagcggct cgtggcgcgg cgcgatgccg
     9241 agttcaccgg cttcgccgct ggcaatgacc agggtcgctt cgccgtggaa gatctcggct
     9301 tcggcgctga cgatgtcgca acggatggtg gtcatgaaga atccgggaac ggggggatag
     9361 ggaacgggga acaggaagct gcgggtctcg aattcagatg cgctgttccc gcttcttcgc
     9421 tcttacgccg ccttcttctc acccgcggcg ttcatctgct cgcccttctg gatggcgtcc
     9481 tcgatgccgc cgaccatgta gaacgcctgc tcgggcaggt ggtccacttc gccgtcgacg
     9541 atcatcttga acgcgcggat ggtttccttg agcggcacgt acttgccggg cgagccggtg
     9601 aacacttccg cgacgtggaa cggctgcgag aagaagcgct cgcacttgcg cgcgcggtac
     9661 acggcctgct tgtcggcttc cgacagttcg tccatgccga ggatcgcgat gatgtccttc
     9721 agctccttgt agcgctgcag ggtgccctgc accttgcggg cgacgtcgta gtgctcctgc
     9781 ccgatcacgt tcgggtcgag ctggcgcgac gtcgacgcca gcggatcgac cgccgggtag
     9841 atgccgagcg acgcgatgtc gcgcgacagc gccacggtcg agtcgaggtg ggcgaaggtc
     9901 gtcgcgggcg acggatcggt gtagtcgtcg gcgggcacgt acaccgcctg gatcgaggtg
     9961 atcgaaccgg tcttggtgga ggtgatacgt tcctgcaggc ggcccatttc gtcggcgagg
    10021 gtcggctggt aacccaccgc cgacggcatg cggccgagca gcgccgagac ctcggtaccg
    10081 gccagcgtgt agcggtagat gttgtcgatg aacatcagca cgtcgcgacc cttgccggag
    10141 gcatcctttt cgtcgcggaa atattcggcc atggtgagtc cggtcagcgc gacacgcagg
    10201 cggttgccgg gcggctcgtt catctggccg tacaccatcg ccaccttgga ttcggggaag
    10261 ttgtcgggct tcacgacgcc ggcctcgatc atctcgtggt agaagtcgtt gccctcgcgg
    10321 gtacgctcgc cgacgccggc gaacaccgac aggcccgagt gctcggtcgc gatgttgttg
    10381 atgagttcga gcatcgtcac cgtcttgccg acgcccgcgc caccgaacag gccgaccttg
    10441 ccgcctctgg cgaacgggca catgaggtcg atgaccttga tgccggtttc gagcagctcg
    10501 ttggcggcgg cctggtcttc gtaggccggc gcggcgcggt ggatttccca ctggtcctcg
    10561 gcgttgaccg ggccccgctc gtcgatcggc tcgcccagta cgttcatgat gcggccgagc
    10621 gtcgcctttc cgaccggcac cttgatcgcc gcgccggtgt tgtcgacgac gaggttgcgc
    10681 ttcaacccgt cggtcgagcc gagcgcgatc gtgcgcacga cgccgtcgcc gagctgctgc
    10741 tggacttcca gcgtcacctg gatgccctgc accttgagcg cgtcgtagac cttcggcacc
    10801 gcatcgcgcg ggaattcgac gtcgacgacc gcgccgatga tttggatgat cttgccttgg
    10861 ctcattgtgt tgtcctcaat ccaattgttg aacccctctc cctccgggag aggggcaggg
    10921 gtgagggttc ggttgtgccg caacgtcacg gccagtgcga accctcatcc ggcgcttcgc
    10981 gccaccttct cccgaaggag aaggaaaaac aaagtcaccc acccaccgcc gcagcgccgc
    11041 cgacgatttc cgaaatttcc tgggtaatcg ccgcctggcg cgccttgttg tagatcaacg
    11101 tcagggtgtc gatggccttg gtggcgttgt cggacgccgc cttcattgcc accatcctcg
    11161 ccgcgtgttc gctggcgagg ttctcgagcg tggcgtggta caccattgcc tcgaagtagc
    11221 ggttcagcac gtgccggatc acgggctccg cctcgggctc gtacaggtag tcccaggtgg
    11281 tcgcgcgttc gatcctggcc gcgctggacg ccggatgcag ttcgtcgccc gcagccgtct
    11341 tgatttcctc ggccaccgcg ggcagcggca gcagtacgtc caccgtcgcc ctctgggtca
    11401 tggtgttcac gaactggttg tagcaaagat agacgcggtc gatcttgccc tgcatgaagg
    11461 cgtcgtcggc gatcttcatc acgccgacca gttgttccag gcgcggacgg tcgcccaagt
    11521 gtgtgaccgt cccgaccagg tccacctgga tgcgccggaa gaattgcgcg gccttctggc
    11581 cgatcgcgat cacctcgacg ccgacgcctt tctggtgcca gccgtggatg tcggccagca
    11641 ccttgcggaa caggttggca ttcaagccgc cgcataggcc ccggtcggtg gaaacgacca
    11701 ggtagcagac gcgcttgacc tcgcgttcgc gcagagccgg atgctggtaa tccggcgtcg
    11761 cctgtgcgat gtgcgcgacc acgttgcgca tgtgcagcga gtacggacgc gacgtccgca
    11821 tcaattcctg cgcgcggcgg atcttggacg cagacaccat ctgcagcgcg ctggtcacct
    11881 tgcgggtgtt cgcgacgctc ttgatcttgc tgcggatttc tctggctccg gccatctcgt
    11941 cctcacgctc gtaggagccg gccatgccgg cgacgaatcg cgcgcatggc gcgctcctac
    12001 atcagttacc agcttccggt cttcttgaat tcgtccagtg ccgacttgaa tgtgccttcg
    12061 atgtcggcat tccagtcgcc ggactcgacg atcttcttca tcaggtcgcc gtggttggct
    12121 tccatgtact ggtgcagcgc gtgctcgaac ggcaggatct tctcgatcgg caggtcgtcc
    12181 aggtaaccct tctcggctgc gtacagcgac accgcgagct gcgcgatggt cagcggcgaa
    12241 tactgcttct gcttcatcag ctcggtgacc cgctggccgc gttccaactg ggcgcgcgtc
    12301 gcggcgtcga ggtcggacgc gaactgtgcg aatgcggcga gctcgcggta ctgcgccagt
    12361 gccagcttca cgccgccgga cagcttcttg acgatcttgg tctgggccgc gccgcccacg
    12421 cgcgacaccg aaatgccggc gttcacggcc ggacggatgc cggcattgaa caggtcggtc
    12481 tcgaggaaga tctggccgtc ggtgatcgag atcacgttgg tcggcacgaa cgcggagacg
    12541 tcgcccgcct gggtctcgat gatcggaagc gcggtcagcg aacccgtctt gcccttcacc
    12601 tcaccgttgg tttccttttc gacgtattcg gcgttgacgc gcgcggcgcg ctcgagcagg
    12661 cgcgaatgga ggtagaacac gtcgcccggg taggcttcgc ggccaggcgg acggcgcagc
    12721 agcagcgaga tctggcgata cgcccacgcc tgcttggtca ggtcgtcgta gatgatcagc
    12781 gcgtcttcgc cgcggtcgcg gaagtattcg cccatcgcgc agccggcgta cggggcgatg
    12841 tactgcatcg tcgccgagtc ggacgccgag gccgcgacga tgatcgtgtg cgcgagcgcg
    12901 tcgtgttcct cgagcttgcg cacgacgttg gcgatggagg attgcttctg gccgatcgcg
    12961 acgtagatgc acttaacgcc ggtgcccttc tggttgatga tcgtgtcgat gcacagcgcc
    13021 gacttgccgg tctggcggtc gccgatcacc agctcgcgct ggccgcggcc gatcgggatc
    13081 atcgagtcca ccgacttgta gccggtctgc accggctgcg acaccgactg gcgccagatc
    13141 acgcccggtg cgaccttctc gaccggcgag gtcgcatgcg tgttcaccgg tcccttgccg
    13201 tcgatcgggt tgccgagggc atcgaccacg cgtccgagca gttcgggacc gaccggcact
    13261 tcgaggatgc ggccggtggt cttggcggtg tcgccttccg agaggtgctc gtactggccg
    13321 agcaccaccg cgccgaccga gtcgcgttcc aggttcagcg ccagcgcgaa ggtgtctccc
    13381 ggcagctcga tcatttcgcc ggccatcgcg tcgttgagcc catggatgcg cacgatgccg
    13441 tcggacacgc acgtgagcgt gccttcgttg cgggtttcgg ccgatagttt gaactggtcg
    13501 atccgcgcgc ggatcagctc gctgatttcg gaagggttga gtgtggtggc catggttttc
    13561 gttcctgtct tgcggcttgc caatcggcag gacgcgggtt ctgcagcatt gatcgtggcg
    13621 ccggtgcgga gaaacgacgc tcgcgtcgct cgctaagacg gaaatgaagc cgcttctccg
    13681 tctttcagtc tcttcgtctg cccgtcttcc cggctctatt gcatcaacgt cgtttcgagt
    13741 tttgccaacc ggctcctgag cgaaccgtct ataacggttt cgccgacgtc gatcacggcg
    13801 ccgcccagga cgcccggatc gatcgtgcgc acgagatcga tgtcgctgcg gtagcggcgc
    13861 ttgagcgcct cgacgagttg cccggcctgc gcgtcgtcca gcggcaccgc gctgcgcacg
    13921 cggactttca gcacgtgttc ggcctcccgc ttcatctgct cgaacagcgc ggcgatttcg
    13981 gggagctcgc gcagccggcg gttgtcggcc agcagctcca ggaaatgcgc gaacgccgaa
    14041 tcggatgcct cgccctcgcg ccgaaacagc gacgccaagg cgcgcggctc gacgcgcgga
    14101 tcgccgaaca gcgccgcgac gcgcggctcg accgacgcct ccgcggcgaa cgcgagcttg
    14161 cccgcccatt cgttctccgc cgcgtgcgcc ttcgcgtatt cgaaggcggc gcgcgcgtag
    14221 ggccgggcga tggtcagctg ctgcgccatg gcgtcagatc cccgccacca gttcgtcgag
    14281 cagcgccttg tgcgtgtcgg cgttgacttc gcggccgagg atcttctcgg caccggcgac
    14341 cgccagcgtg gcgacctgct cgcgcagctc cgcacgcgca cgatgggcca tgttctcgat
    14401 gtcggcttgc gcggcagcct tctgccgcgc cgcctccagc agcgcatcgg cacgcgcctt
    14461 gtcgacgatc tgcccggcct gctggttggc ccgctcgatg atcgacgcgg cttcggcccg
    14521 cgccttgcgg atctcgtcgg cggcgtgggt gtcggcgtcg gcgagttcac ggcgcgcgcg
    14581 atcggccgct tcgaggcctt cctcgatcct gcgctggcgc tcctcgatgg cacggatcag
    14641 cggcggccac acgaacttga gcgtcagcgt gatcatgatc gcgaacgcga ttatctcgcc
    14701 gatcagggtt gcattgattt ccatcgcgtc actacctgta cggagtcggt gctacagggg
    14761 ccgcgacctc tcagggcgcc gccgccgcgg c
//
LOCUS       DASSNH010000312         9879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_429137, whole genome shotgun sequence.
ACCESSION   DASSNH010000312 DASSNH010000000
VERSION     DASSNH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429137"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VFK00_11700"
     CDS             <1..901
                     /locus_tag="VFK00_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7593651.1"
                     /translation="DLPVWNKDVLVYEVYNTDGKPLGLFYADYFKRDNKAGGAWMSSL
                     VHPSKLLDHSPVIYNVANFTKPAEGQPALLSMDDVITMFHEFGHALHGFFSEGKYPSL
                     SGTDTARDWVEFPSQFNEHWATYPEVFDHYAKNYKTGAPMPEELAAKLQKSRLFNKGY
                     DMTELLEAALLDMGWHTLPASAPQVGDVDAFETKALEDNHILVPYVPPRYRSSYFLHI
                     WGNGYASGYYAYLWTQMLADDAYGWFEEHGGLTRANGDRFRQMILSRGNTEDLAKLYE
                     AWRGRAPNIDAMKKDRGLEPQKP"
     gene            1317..1892
                     /locus_tag="VFK00_11705"
     CDS             1317..1892
                     /locus_tag="VFK00_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7593652.1"
                     /translation="MSTATTKTMKTFRRAAVGAALVLFAMTAHAFPIATPGTEGLKVI
                     VSGTDHVIATYEGNSAAYSNDLLLNGVFIFNNHTTPVGTSVDLGSFAPGTELVFQLHV
                     NNTGYDFYTGPDSRNPDSHAHARVQADWEPGTTLVSFEDLFNGPFEYNDLSFSFTNTT
                     SSATPVPEPSPLLLMLAGIGLIGLMVRLRRA"
     gene            complement(1959..2162)
                     /locus_tag="VFK00_11710"
     CDS             complement(1959..2162)
                     /locus_tag="VFK00_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593653.1"
                     /translation="MLDGPPDMQVVLAASNLERRHRLDARNASPVVAVGPPARRCTGE
                     ISENTRARARRTSAAQGTHRIQG"
     gene            2518..3435
                     /locus_tag="VFK00_11715"
     CDS             2518..3435
                     /locus_tag="VFK00_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HET7593654.1"
                     /translation="MVITSPAAFLSALPGLSPLPDAQATARAAFLVAPAAAGLASESA
                     RDNRYMQIDAPFDAQRALVQHAALAQALREDVPTIVFPGDPSTPDAMFPNNVFATTSG
                     RLVVGRMRHGVRQREAGRADIRGFFRVVLGYAETDLSQHEDCVAELTGSLVIDHARGV
                     GYCGLSERCDMAGARAMHDAFGLRLMFCFELAEGEYHTNVVMASLAGRAVMLAPDGFR
                     ERAVPEAIAKAHGDREVRLRPGQKRAYAGNAITLSEDRVWMSERAAAALDDTQRAALA
                     SWGFRIGTVPLDEIEKAGGSLRCCVAEIF"
     gene            complement(3457..4821)
                     /locus_tag="VFK00_11720"
     CDS             complement(3457..4821)
                     /locus_tag="VFK00_11720"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7593655.1"
                     /translation="MSGPSHVAEQPIERHAQLAEYLAAGGRPAEQWRIGTEHEKFVFR
                     TDDLEPPPYAGERGIGALLRGIEGCGWEPVEEGGNLIALSRGAASVTLEPAGQLELSG
                     APVETIHQTCNEVQEHLHCVRRASEPLGLGILGMGFQPKWKRADMPWMPKARYDIMRR
                     YMPKVGSLGLDMMTRTCTVQVNLDFADEADMVKKFRVSLALQPIATALFADSPFTEGK
                     PNGYLSYRSHIWADTDADRTGMLDFVFEDGFGFERYVDYLLDVPMYFVHRHGKYVDCA
                     GQSFRDFLAGKLPALPGEKPTLRDWSDHMTTAFPEVRLKQYLEMRGADGGPWNRLCAL
                     PAFWVGLLYDATALDAAWDLVRDFSLAERNALRDGVPERALKLPFRGETVRELALRAL
                     EISGNGLKRRARLNPNGADETIFLEPLIEFAEANQTPAERKLELFHGAWNGSVDPVFR
                     EFAY"
     gene            complement(4958..5563)
                     /gene="yihA"
                     /locus_tag="VFK00_11725"
     CDS             complement(4958..5563)
                     /gene="yihA"
                     /locus_tag="VFK00_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET7593656.1"
                     /translation="MQVSENNPLGGATFVLAAHRPDQLPADEGAEVAFAGRSNAGKSS
                     AINALAGQASLARTSRTPGRTQQLVVFALPDGRRLVDLPGYGYAKVPPALRDHWRQAI
                     DAYLRRRRSLRGLVLVADVRHALTPFDRQMLAFCASGDLPCVVLLSKADKLSRAQLGT
                     RQREVGQEITKQGWRAATIPLSATAGTGVAEARAAVVALLS"
     gene            5800..6615
                     /locus_tag="VFK00_11730"
     CDS             5800..6615
                     /locus_tag="VFK00_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7593657.1"
                     /translation="MGFRRSLAFIVVLAAAAPLAVLSAPPADQSAATPAAASSSAPAS
                     GASAAAPASAASATDAAEAEFGKLAAAQTRPGDPKAGQGKAAACGACHGMDGNSTDPQ
                     YPKLAAQSEQYIVTQLMRFKTGLRQNAIMQGIAAPLSPQDMHDLGAYFSQQKRLPGVA
                     DEKLAQAGEKLYREGDPSRGVPSCMGCHGPDGAGNPGWRVPNIGGQHADYVQKQLQAW
                     HDGTSWGSDAHAKIMPTIAQRLTEQDISAVSSYVEGLHGTPPPETSAAPSPID"
     gene            6687..7388
                     /locus_tag="VFK00_11735"
     CDS             6687..7388
                     /locus_tag="VFK00_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HET7593658.1"
                     /translation="MTKRIAAFLSLAAVAGVAFAQAAPQQWVEGKNYFAIPNPQPTNQ
                     PDKVVVTEVFSFACPACNAFQPYIDKLEAELPQGAVLEFVPASFIPTEDWPVFQRAYF
                     TAKALGVDTQQSHDAMFKAIWGPNGSLATYDQATQRPKSKDNMPTIQDVAKFYANYGA
                     KPDEFVAAANSFSVNLEMKRADQQIVTWGVNSTPTIVVDGKWRADSRSANGAQNLVDL
                     TLYLANRELAAKKGQ"
     gene            7390..8154
                     /locus_tag="VFK00_11740"
     CDS             7390..8154
                     /locus_tag="VFK00_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HET7593659.1"
                     /translation="MRRAYGLLGAALLACGLLLTAACSAQDDGTPGSADAPATAASVA
                     STAAPAATPAPATSTHFTEGDQYVTLNLPEGQAAPTGPVELVEVFSYGCPHCAQFAPY
                     MDKLRAQLPKDVQVRYMPAMFSPAWMPYAQAFYAARQLGVLEQTHDKMFQAMLEHYPL
                     NSLQDMATWYGRHGVDPQKFIAAATNAQTTQQMTSDRKTEMSWGIDATPTLVVGRRAS
                     DAKDAPFVALMRSADIQSYAQLQQVGLWMVRHAGQP"
     gene            8228..9019
                     /locus_tag="VFK00_11745"
     CDS             8228..9019
                     /locus_tag="VFK00_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7593660.1"
                     /translation="MNAEPAAAPVDNVLRILSCNILAGASVTRYHEYFTRSWGAVLPG
                     KYKLGNLDRLAESIAKFDVVGLQEADAGSIRSGFLNQTRYLAETAGLPYWSHQPNRKL
                     SNVSYTANGLISRAAPTEVLDYPLPGRLGGRGVLLARYGEGSDALTVAIAHLSLSVRS
                     RLRQIDFIAELLHDRPNVVLMGDLNCAPDCPELNTLFAHTSLVPPADPRPTFPSWRPQ
                     RAIDHILVTSHLAIERMWTLPAAFSDHLPLAAEIRVPEAAWKAAA"
     gene            9047..>9879
                     /locus_tag="VFK00_11750"
     CDS             9047..>9879
                     /locus_tag="VFK00_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET7593661.1"
                     /translation="MEILTRTRATWRACMYTAVAIAIGTFAAFGAAQTPVAQTTASST
                     TLQQQRESFRAAYAAARTGAAWRPLANGLESYPLYPYLEAAALQHDIKNATRAEIDAY
                     LQRYAGLIPAGDLREAELRWLAKQKDWTGFQHFYRPGLGDTLTCDALQARLAQGEPLD
                     FERDLAELWQQTSLPSACQPVLDAAFVQGLLTPQRVWDRIERAADAGRSSTISQSAAW
                     LTGDDARAANRIADAVASPADFLKKADTLADTPRNREAVSRALTRFARRHSAKAIDDW
                     RS"
BASE COUNT         1699 a         3401 c         3210 g         1569 t
ORIGIN      
        1 cgacctgccg gtgtggaaca aggacgtgct ggtgtacgag gtctacaaca ccgacggcaa
       61 gccgctcggc ctgttctacg ccgactactt caagcgcgac aacaaggccg gcggcgcgtg
      121 gatgagcagc ctcgtgcatc cgtccaagct gctggaccat tcgccggtga tctacaacgt
      181 cgccaatttc accaagccgg ccgagggcca gcctgcgctg ttgtcgatgg acgacgtgat
      241 cacgatgttc cacgagttcg gccatgcgct gcacgggttc ttctcggaag ggaaataccc
      301 gagcctgtcc ggcaccgaca ccgcacgcga ctgggtggag ttcccctcgc agttcaacga
      361 gcactgggcg acctatcccg aggtgttcga ccactacgcg aagaactaca agaccggtgc
      421 gccgatgccc gaggaactgg ccgccaagct ccagaagtcg cggctgttca acaagggcta
      481 cgacatgacc gagctgcttg aagccgccct gctcgacatg ggctggcaca ccctgcccgc
      541 cagcgccccg caggtcggcg acgtcgatgc attcgaaacc aaggcgctcg aggacaacca
      601 catcctcgtt ccctacgtgc cgccgcgcta ccgttcgagc tacttcctgc acatctgggg
      661 caacggctat gcgtccggct actacgcgta tctctggacc cagatgctgg ccgacgatgc
      721 ctacggctgg ttcgaggaac acggcggcct gacccgcgcc aacggcgacc gcttccgcca
      781 gatgatcctt tcgcgcggca acaccgagga tctcgccaag ctctacgagg cctggcgcgg
      841 ccgcgcaccc aacatcgacg cgatgaagaa ggaccgcgga ctggagccgc agaagccgta
      901 ggagcggccc atggccgccc atggggttcc ggtgattcga ggaaaaggcc cgctaccgcg
      961 ggcctttttt tcttgctcgc ggcgccgact gcagcaggct ccgaaataaa tccgcacagt
     1021 cgaaggttcc agccgacgcc accggccgcc gaccctgacg gagaacctta cattccgcgg
     1081 tcctggtcaa acatcaagcg cgacaagcgc cgcagccgaa aaagcgcgaa cgccctgcat
     1141 gcgacctggt cgccgaactt tacattccgg aattcgcttt gaaaataatc ctcggaaaaa
     1201 cagccgcctg cgttttggca tgaaacttga tttgctgatt cgaccgcggc acatgctcgc
     1261 ggctgcgtcg attgtggccg ggaatcgttt cccgactccg acatggaggt aacggtatga
     1321 gcactgcgac aacgaagacc atgaagacat tcaggcgtgc cgctgtcggc gccgcgttgg
     1381 tgcttttcgc gatgacagcc catgcttttc ccatcgccac ccccggaacg gaggggctga
     1441 aggtcatcgt cagtggcacc gaccacgtga ttgcgacgta cgagggcaac tcggccgcct
     1501 acagcaatga tttgctgttg aacggcgttt tcattttcaa caaccatacg acgccggtcg
     1561 gcacgagtgt cgacttgggc agcttcgccc ccgggaccga actcgtattc cagcttcacg
     1621 tgaacaacac gggttatgac ttctataccg ggcccgattc gcggaacccc gactcgcatg
     1681 cacatgcgcg ggtccaggcc gactgggagc ccggcacgac attggtcagc ttcgaagacc
     1741 tgttcaacgg cccgttcgaa tacaacgacc tgagcttctc cttcaccaat acgacgtcgt
     1801 cggccacgcc ggtacccgag ccgagcccgc tcttgctcat gctggccggg attgggctga
     1861 tcggcctgat ggtgcggttg cgccgagcct agctgcgcga gtccagcgcg gtctcaaacg
     1921 gcagccctcc cgcctcgggc ggtgcaggtt ccactggatc agccttgaat ccggtgggtt
     1981 ccttgcgcgg cgctcgtccg ccgtgcgcgg gcgcgcgtat tttcggagat ctcgccggtg
     2041 cagcgccgtg ccggcggccc cacggccacc accggactgg cgttgcgcgc atcgagccgg
     2101 tggcggcgtt cgaggttcga ggcggccagt accacctgca tgtcgggagg gccgtccagc
     2161 aacggctcca gcccacttcc ggaaccaaga cgcgacagcg ctgccagtaa tgcgggcgga
     2221 gcagacgccg caggagcgtc agcagcacca tgtcctcgag gcaacctgaa cggacaacga
     2281 gggggccgtg ggaaaacatg caatacccgc gctggtggag ggcggcgggg tatcgggaga
     2341 gaggccgtat gatttgcttg gctggcgatt gcgcaggagt ttccgtcgtg ttcgttcggc
     2401 ggccgagttc gcggaggcac cggggaaacc caggccgtgg ccagccagcc ttgcgcaccg
     2461 gaagcgcgga accgcatccc gcattgcgcc tgacagcgcc gtgacaaatc gagccccgtg
     2521 gtcatcacct ctcctgccgc cttcctgtcc gccctcccgg ggctctcgcc gttgccggat
     2581 gcgcaggcga ccgcccgtgc ggcgttcctg gtggcgccgg cggcggcagg cttggcatcg
     2641 gagtccgcgc gcgacaaccg ctacatgcaa atcgatgcgc cgttcgacgc gcaacgggcg
     2701 ctcgtacagc acgcagcgct cgcgcaggcg ctgcgcgagg acgttccaac gatcgtgttt
     2761 cccggcgacc cgtccacgcc cgacgcgatg ttcccgaaca acgtattcgc gacgacatcc
     2821 ggccggctgg tcgtcgggcg gatgcgtcat ggcgtccgcc aacgcgaagc gggccgcgcc
     2881 gacatccgcg gcttcttccg cgtcgtgctc ggctacgccg aaaccgacct ctcgcagcac
     2941 gaggattgcg tggcggaact caccggatcg ctggtgatcg accacgcgcg cggcgtcggc
     3001 tactgcggac tatccgagcg ttgcgacatg gccggcgcac gcgcgatgca cgacgcgttc
     3061 ggcctgcgcc tcatgttctg tttcgagctc gcggaaggcg aataccacac caacgtcgtg
     3121 atggcatcgc tcgccggccg cgcggtgatg ctcgcccccg acggcttccg cgagcgggcg
     3181 gtgccggagg cgatcgcaaa ggcacatggc gatcgcgaag tccggctgag gcccgggcag
     3241 aagcgcgcct acgccggcaa cgcgatcacc ctgagcgaag atcgcgtgtg gatgagcgaa
     3301 cgtgcggcag ccgcactcga cgacacgcaa cgcgctgcgc tggcgtcgtg gggcttccgc
     3361 atcggcaccg tgccgctcga cgaaatcgag aaggccggcg gcagcctgcg ctgctgcgtc
     3421 gcagaaatct tctgatcggc gcgaagctgg ccgcggtcag tacgcgaatt cgcggaacac
     3481 cgggtcgacg ctcccgttcc acgcaccgtg gaacaattcc agcttgcgtt cggcgggcgt
     3541 ctggttggcc tcggcaaact cgatcagggg ttcgaggaag atcgtctcgt cggcgccatt
     3601 ggggttgagc cgcgcgcgac gcttcagtcc attccccgaa atctccagcg cgcgcagtgc
     3661 aagctcgcgc accgtttcgc cgcgaaacgg caacttcaac gcgcgttccg gcacgccatc
     3721 gcgcagcgca ttccgttcgg cgagggagaa atccctcacc aggtcccacg ccgcatccag
     3781 cgccgtcgcg tcgtacaaca ggccgaccca gaacgccggc agtgcgcaca gccgattcca
     3841 cggcccgccg tccgcaccgc gcatctcgag gtattgcttg agccgcactt ccgggaaggc
     3901 agtggtcatg tggtcggacc agtcgcgcaa ggtgggcttc tcgcccggca gcgcgggcag
     3961 cttgcctgcc aggaagtcgc ggaaggactg tccggcgcag tcgacgtact tgccgtggcg
     4021 gtgcacgaag tacatcggca cgtccagcag gtaatcgacg tagcgctcga acccgaagcc
     4081 gtcctcgaac acgaagtcga gcattccggt gcgatcggcg tcggtatcgg cccagatgtg
     4141 cgagcgatag ctcaagtacc cattgggctt tccctcggtg aacggcgaat cggcgaacaa
     4201 cgcggtcgcg atcggctgga gggccagcga tacccggaac ttcttcacca tgtcggcttc
     4261 gtccgcgaag tcgaggttga cctgcacggt gcaggtgcgg gtcatcatgt cgagtccgag
     4321 cgagccgacc ttgggcatgt agcggcgcat gatgtcgtag cgggccttgg gcatccaagg
     4381 catgtccgcg cgcttccact tgggctggaa ccccatgccg aggatgccga ggccgagcgg
     4441 ttcggacgcc cgccgcacgc agtgcaggtg ttcctgcacc tcgttgcagg tctggtggat
     4501 ggtttccacc ggggcgccgg acagttccag ttgccctgcc ggctcaagcg tcaccgaagc
     4561 cgcgccgcgg ctgagtgcga tcaggttgcc gccttcctcc acgggctccc agccgcagcc
     4621 ctcgataccc cgcaacagcg cgccgatgcc gcgctcgccc gcgtacggcg gcggctccag
     4681 gtcatcggtg cggaacacga acttctcgtg ttcggtcccg atcctccact gctccgccgg
     4741 gcgccccccg gcggccaggt actccgcgag ttgcgcgtgc cgctcgatcg gttgttccgc
     4801 aacatggctg ggaccggaca tgcacgcacc tttgcgaggg caagggcgga aagatggggc
     4861 ggcggcgggc gatccgcaag agcccgactg ttgccggtgg aggcgcgcac catgcgcacg
     4921 atggaggcgc cagcccgatc gcgcgcgtgc cgcgctccta cgaaagcagc gccaccacgg
     4981 cggcgcgtgc ctcggcgacg ccggtgcccg cggtcgccga gagcgggatg gtggcggccc
     5041 gccagccctg cttcgtgatt tcctggccga cctcccgctg ccgcgtcccg agctgcgcgc
     5101 gcgagagctt gtccgccttg ctgagcagca cgacgcaggg cagatcgccc gatgcgcaga
     5161 acgccagcat ctgccgatcg aagggcgtca gggcgtggcg cacatcggcg accagtacca
     5221 gtccgcgcag cgaccgccgg cggcgcaggt aagcatcaat cgcctggcgc caatgatcgc
     5281 gcagcgccgg gggaaccttg gcatagccat agcccggcag gtccaccagc cgcctgccgt
     5341 ccggcaacgc gaacaccacg agttgctggg tgcgtccggg cgtccggctg gtgcgcgcaa
     5401 ggctcgcctg cccggccagc gcattgatgg cgctggactt cccggcattg gagcggccgg
     5461 cgaacgcgac ttccgcgccc tcgtccgccg gcaactggtc gggccggtgc gcggcgagga
     5521 cgaaggtggc gccgcccaag gggttgtttt ccgagacttg catggctggg ctgtccgggt
     5581 cgcgggtttc gtcggcttct tgtctcgtcg tggggattcc cgctgcaacg ttcgccttca
     5641 ggcgcttgca gcggcctggt cgcgcttgtt gccggcagct accccgcggg tgcccgacat
     5701 ggcaggtttg acgcatcttt gttgcagcag cgataatccg gcggttcacg ctggcgcgcg
     5761 gcgtcagtgt ccatgccttt gcgggaaaga gaggattgta tgggtttccg gcgttcgctt
     5821 gccttcattg tcgtgctcgc cgccgccgca ccgctcgcgg tgctgtccgc gccgcctgcg
     5881 gatcaatccg cggcgacacc ggcggcggct tcctcctcgg cacccgcgag cggcgccagc
     5941 gcggccgcgc cggccagcgc cgcgagcgca accgacgccg ccgaagcgga attcggcaag
     6001 ctcgcggccg cgcaaaccag gccgggcgat cccaaggcag gtcaaggcaa ggcggcggcc
     6061 tgtggcgctt gccacgggat ggacggcaac tcaacggacc cgcagtatcc gaagctcgcg
     6121 gcccagagcg agcagtacat cgtcacccag ctgatgcgct tcaagaccgg attgcggcag
     6181 aacgccatca tgcagggcat agcggcaccc ttgtcgccgc aggacatgca tgacctcggc
     6241 gcctacttct cccagcagaa gcgcctgcct ggcgtcgcgg acgagaagct cgcgcaggcc
     6301 ggtgagaagc tgtaccgcga aggcgacccg agccgcggcg ttccctcctg catgggctgc
     6361 cacggccccg acggcgcggg caatcccggc tggcgcgtcc ccaacatcgg cggccagcat
     6421 gcggattacg tgcaaaagca gttgcaggcg tggcatgacg gcacgagctg gggcagcgac
     6481 gcacacgcga agatcatgcc gacgatcgcg cagcgcctga ccgagcagga catttcggcg
     6541 gtatccagct atgtcgaggg gttgcacggc accccgccac cggaaacctc ggccgcgccc
     6601 tcgcccatcg attgaacccg gcgggcccgg cgtggtcgat tcaggcgcgc tgttgcagac
     6661 tccaccctaa cgccaggaat ccgatcatga ccaaacgcat tgccgcattt ctgtcgctcg
     6721 ccgccgtggc cggcgtcgcc ttcgcacagg ccgcgccgca gcagtgggtc gagggcaaga
     6781 actacttcgc gattccgaac ccgcagccca ccaaccagcc cgacaaggtg gtggtgaccg
     6841 aggtgttctc gttcgcctgc ccggcctgca acgccttcca gccctacatc gacaagctcg
     6901 aagccgaact gccccagggc gcggtgctcg agttcgtgcc ggcctcattc atccccactg
     6961 aggattggcc ggtgttccag cgcgcctact tcaccgccaa ggcgctcggc gtcgacaccc
     7021 agcagtcgca cgacgcgatg ttcaaggcca tctggggtcc gaacggctcg ctcgccacct
     7081 acgaccaggc cacccagcgg ccgaagtcga aggacaacat gccgaccatt caggacgtcg
     7141 cgaagttcta tgcaaactac ggcgccaagc cggacgagtt cgtcgcggcc gccaattcat
     7201 tttcggtgaa cctcgaaatg aaacgcgccg accagcagat cgtcacctgg ggcgtcaaca
     7261 gcacgccgac gatcgtggtg gacggcaaat ggcgcgccga ttcgcgctcg gccaatggcg
     7321 cgcagaacct ggtggacctg acgctgtacc tggccaacag ggaactcgcc gcaaagaagg
     7381 gacagtgaga tgaggcgcgc atacggcctg ctcggcgccg cgctgctggc ctgcggcctg
     7441 ctgctcaccg cggcctgctc ggcgcaggac gacggcaccc ccggcagcgc tgacgctccg
     7501 gcgaccgccg ccagcgtggc gagcacggct gcccctgccg cgacgcccgc accggcgacc
     7561 tccacccact tcaccgaagg cgaccaatac gtcaccctga atctgcccga agggcaagcc
     7621 gcgccgaccg gcccggtcga actggtcgag gtgttctcgt acggttgccc gcactgcgcg
     7681 caattcgcgc cgtacatgga caagctgcgc gcgcagttgc cgaaggacgt gcaagtccgc
     7741 tacatgccgg cgatgttcag cccggcatgg atgccgtacg cgcaggcgtt ctatgcggcg
     7801 cggcagttgg gcgtgctcga gcagacgcac gacaagatgt tccaggcgat gctggaacac
     7861 tacccgctca attcgttgca ggacatggcg acgtggtacg gccgccacgg tgtcgatccg
     7921 cagaagttca tcgcggcggc caccaatgcg caaaccaccc agcaaatgac ctctgaccgg
     7981 aagacggaga tgtcctgggg catcgacgcg actccgacgc tggtggtggg gcgccgggcg
     8041 agcgacgcca aggatgcgcc gttcgtcgcg ctgatgcgca gcgccgacat ccagagttac
     8101 gcacagttgc agcaggtcgg cctgtggatg gtgcgccacg ccgggcagcc ttgagccgcg
     8161 ccgccccggc tcacttgtgt tccggcgcac gcgacgccac atttcatggt gttaatctgg
     8221 tcgagccatg aatgccgagc ccgctgccgc gccggtcgac aacgtcctgc gcatcctgag
     8281 ctgcaacatc ctcgccggcg cgagcgtgac gcgctaccac gaatacttca cgcgtagctg
     8341 gggcgcggtg ctgccgggca agtacaagct cggcaacctc gaccggctgg cggaatcgat
     8401 cgcgaaattc gacgtggtgg ggctgcagga ggccgacgcc ggcagcatcc gttcgggctt
     8461 cctcaaccag acccgctacc tcgccgaaac ggcggggttg ccgtactgga gccaccagcc
     8521 gaatcgcaag ctgtcgaatg tctcctacac cgccaacggg ttgatcagcc gcgccgcgcc
     8581 gacggaggtg ctcgactatc ccctgccggg ccgtctgggc ggacgcgggg tcctgttggc
     8641 ccgctatggc gaaggctccg atgcgctgac ggtggcgatc gcgcaccttt cgctcagcgt
     8701 gcgatcgcgc ctgcgccaga tcgatttcat cgccgaactg ttgcacgacc gccccaacgt
     8761 cgtgctgatg ggcgacctga actgcgcgcc ggactgtccg gaattgaata ccctgttcgc
     8821 gcacacgtcg ctggtgccgc ccgccgaccc gcggccgacc ttcccgagct ggcgcccgca
     8881 acgggccatc gatcacatcc tcgtcacgtc gcatctcgcg atcgagcgga tgtggacgct
     8941 gccggcggcg ttctccgacc acctgccgct cgcggcggag atccgggtgc cggaggccgc
     9001 gtggaaggcg gcggcgtagt gcgctgcatc ctctagaatc ggtgggatgg aaatcctgac
     9061 ccgcacgcgc gcgacctggc gcgcctgcat gtacaccgcc gttgcgatcg ccatcggcac
     9121 cttcgcggcg ttcggcgccg cgcagacgcc cgtcgcgcaa accacggcca gctcgaccac
     9181 cctccagcag cagcgcgagt cattccgcgc cgcgtatgcg gcggcccgga cgggagcagc
     9241 gtggcggcca ctggcgaacg gcctcgagag ctatccgctc tatccctatc tcgaagcggc
     9301 cgcgttgcag cacgacatca agaatgcgac gcgcgccgag atcgatgcct acctgcagcg
     9361 atacgcgggt ttgatcccgg ccggcgacct gcgcgaggcc gagctccgct ggctggcaaa
     9421 gcagaaggac tggacaggtt tccagcattt ctaccgaccc ggcctcggcg atacgctgac
     9481 ctgcgatgca ttgcaggccc gcctcgcgca aggcgagccg ctcgacttcg aacgcgacct
     9541 cgcggagctg tggcagcaaa ccagcctgcc ctcggcatgc cagccggtgc tcgatgccgc
     9601 cttcgtgcag ggcctgctga ccccgcaacg cgtgtgggac cgcatcgaac gcgcggccga
     9661 cgccgggagg tcctcgacca tcagccaatc cgccgcgtgg ctgacgggtg atgacgcccg
     9721 tgccgcgaac cggatcgccg acgcggtcgc atcaccggcg gacttcctga agaaagccga
     9781 tacgcttgcc gacaccccgc ggaaccgcga agcggtgtcc cgcgcgttga cccggttcgc
     9841 gcggcgacat agcgcgaagg ccattgacga ctggcgatc
//
LOCUS       DASSNH010000313         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_986814, whole genome shotgun sequence.
ACCESSION   DASSNH010000313 DASSNH010000000
VERSION     DASSNH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986814"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFK00_11755"
     CDS             <1..858
                     /locus_tag="VFK00_11755"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7593662.1"
                     /translation="FARVFNILHGQHGGGEDGVLQGALDSLHVPYTGSGVLGSALSMD
                     KVRSKWVWTALGLPTPKFVPLPRGATGSSIHEAARAVGFPLIVKPASEGSSVGVSRVF
                     DERDLDGAVALAQRYPGDLLMETLVEGGEFTVSILGRDVLPSIRIVPKGEYYDYNAKY
                     VAEDTQYICPGLEGGAEAGLRSLALQAFDALGCSGWGRVDMMRDAQGQPWLLEVNTAP
                     GMTSHSLVPKAAGAAGMDYAELCWRVLETSFDEEQGTGNGEQGVAGAGFSSPFPVPGS
                     RSSDQGDAP"
     gene            855..1619
                     /locus_tag="VFK00_11760"
     CDS             855..1619
                     /locus_tag="VFK00_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HET7593663.1"
                     /translation="MKGIVSLRLAAWLIALTLIALPIVGVLEGWFASDRWPVRQLQVH
                     ATFRHVSAAQVRAAVKPALGAGFFAVDLDHVRDAVAALPWVGRVEVSKHWPDALDITI
                     AEIQPIAHWGDGALLDRDGRIFKVPDAGIVHGLPSFNAPDDRVADVMAFYRTAETDFA
                     PYRLRVEQVDLAARGSWTLVLSNGGRVAVGGDRPDQRLARFAAALPVLMRGRSDGFVY
                     ADLRYSNGFAVRWPEPAPAVPPNPTNGASHAADGNV"
     gene            1624..2862
                     /gene="ftsA"
                     /locus_tag="VFK00_11765"
     CDS             1624..2862
                     /gene="ftsA"
                     /locus_tag="VFK00_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7593664.1"
                     /translation="MNRKSDKNLVVGLDIGTSKVMAIVGEYLPGEPVEVIGFGSHTSR
                     GMKKGVVVEIDSNVHSIQRAIEEAEQMAGCDIRSVCASISGSHLETHNSRGAAAIRDR
                     EVGAGDLDQVLESASAVPISADRKVLFKEPQEYRIDGQDGIRHPVGMSGVRLEAGVHL
                     VTGAASAVQNLTKCIQRCGLQVDDLVPAAVAASKAVLTEDERELGVCVVDLGAGTTDV
                     AIYTHGSIRYTKSLPVGGDQVTNDISQYVQTPTANAEEIKVKYGCALAQMARGEETIQ
                     VPSVGDRPPRRLQRQALAQSIQARYEEIFEMVQDELRRSGYENLLAAGVVLTGGGSRI
                     EGALYLAEEVFHKMVRLGSPQHVTGATEVIATPANATGIGLLLHGSREGASNRVMGGA
                     VGDSVAGAIGKMKGWLSRNF"
     gene            2992..>3113
                     /locus_tag="VFK00_11770"
     CDS             2992..>3113
                     /locus_tag="VFK00_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972761.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7593665.1"
                     /translation="MFELVEKLAPNAVIKVIGVGGGGGNAVGHMVNSTIDGVDF"
BASE COUNT          510 a         1027 c         1093 g          483 t
ORIGIN      
        1 ttcgcgcggg tgttcaacat cctgcacggc cagcacggcg gtggcgagga cggcgtgctg
       61 cagggcgcac tggattccct gcacgttccc tacaccggct cgggcgtgct cggctcggcg
      121 ctgtcgatgg acaaggtgcg cagcaagtgg gtctggaccg cactgggcct ccccacgccg
      181 aaattcgtgc cattgccgcg gggcgccacc ggcagctcga tccatgaagc cgcgcgcgcc
      241 gtcggctttc cgctgatcgt gaaacccgcc agcgagggat cgagcgtcgg cgtatcgcgg
      301 gtattcgacg agcgcgacct cgatggcgcg gtcgcgctcg cgcagcgtta tccgggcgac
      361 ctcctgatgg agacgctggt cgagggcggc gaattcaccg tctccatcct cggccgggac
      421 gtgttgccca gcatccggat cgtgccgaag ggcgagtact acgactacaa cgccaagtac
      481 gtcgccgagg acacccaata catctgcccc gggctcgagg gtggcgccga agccggattg
      541 cggtcgttgg cgttgcaagc cttcgacgcg ctcggctgct cgggctgggg ccgcgtggac
      601 atgatgcgcg acgcgcaggg ccagccctgg ctgctggagg tcaacaccgc gcccggcatg
      661 acctcgcact cgctggtgcc gaaggccgcg ggcgctgccg gcatggatta cgccgagttg
      721 tgctggcggg tattggagac gagcttcgac gaagagcagg gaacagggaa tggggaacag
      781 ggagtggcgg gcgccggttt ttcttccccg ttcccggttc ccggttcccg ttcttccgac
      841 cagggagacg ccccatgaag ggcatcgtct ccttgcgcct cgccgcctgg ctgatcgcgc
      901 tcacgctgat cgcgttgccg atcgtcggtg tgctcgaagg ctggttcgcc tcggaccgct
      961 ggccggtgcg gcaactgcag gtgcacgcga cgttccggca tgtcagcgcc gcgcaggtgc
     1021 gcgccgcggt gaagcccgcg ctcggcgccg gcttcttcgc ggtcgacctc gaccacgtgc
     1081 gcgacgcggt ggctgcgctg ccgtgggtcg gccgggtcga ggtcagcaag cattggcccg
     1141 acgccctcga catcaccatc gccgagatcc agccgatcgc gcactggggc gacggcgcat
     1201 tgctcgatcg cgacggccgg atcttcaagg tgccggacgc cggcatcgtc cacggcttgc
     1261 cgagcttcaa tgcgcccgat gaccgcgtcg ccgacgtgat ggcgttctac cgcaccgccg
     1321 aaaccgactt cgcgccgtat cgcttgcgcg tcgaacaggt ggatctcgcc gcacgcggca
     1381 gctggacgct ggtgctttcc aatggcggcc gggtcgcggt cggtggcgac cgcccggacc
     1441 agcggctcgc ccgtttcgcc gcggccctgc ccgtgctgat gcgcgggcgc agtgacggct
     1501 tcgtgtacgc cgatcttcgc tacagcaatg gtttcgcggt gcgctggccc gagccggcgc
     1561 ccgccgttcc cccgaacccg acgaatggtg cttcccatgc tgctgacgga aacgtttaga
     1621 cccatgaacc gcaagagcga caagaacctg gtggtcggcc tcgacatcgg cacctcgaag
     1681 gtgatggcga tcgtcggcga atacctgccg ggcgagccgg tcgaggtgat cgggttcggc
     1741 agccacacat cgcgcggcat gaagaagggc gtggtggtcg aaatcgactc caacgtgcat
     1801 tcgatccagc gcgcgatcga ggaagccgag cagatggcag gctgcgacat ccgttcggtg
     1861 tgcgcctcga tttccggcag ccacctcgag acccacaact cgcgcggcgc cgccgcgatc
     1921 cgcgaccgcg aagtgggtgc cggcgacctc gaccaggtgc tggaatcggc cagcgcggtg
     1981 ccgatctcgg ccgatcgcaa ggtgctcttc aaggaacctc aggaataccg catcgacggc
     2041 caggacggca tccgccaccc ggtcgggatg agcggcgtcc gcctggaagc cggagtgcac
     2101 ctggtgaccg gcgcggcttc ggcggtgcag aacctcacca agtgcatcca gcggtgcgga
     2161 ctgcaggtgg acgacctggt gcccgcggcg gtcgcggcgt ccaaggccgt cctgaccgaa
     2221 gacgagcgcg aactcggcgt gtgcgtggtc gacctcggcg ccggcaccac cgacgtcgcg
     2281 atctatacgc acgggtcgat ccgctacacc aagtcgctgc cggtcggtgg cgaccaggtg
     2341 accaacgaca tctcgcagta cgtgcagacg ccgaccgcga acgccgagga gatcaaggtg
     2401 aagtacggct gcgcactggc ccagatggcg cgcggcgagg aaaccatcca ggtgcccagc
     2461 gtgggcgacc gtccgccccg tcgcctgcag cggcaggcgc tcgcgcagtc gatccaggcc
     2521 cgctacgagg agatcttcga gatggtgcag gacgaactgc gccgctccgg ctacgagaac
     2581 ctgctggcgg cgggcgtggt gctgaccggc ggcggatccc gcatcgaggg cgcgctgtac
     2641 ctcgccgagg aggtcttcca caagatggtg cggctcggct cgccgcagca cgtcaccggc
     2701 gccaccgaag tgatcgcgac gcccgccaac gccaccggca tcgggttgct gctgcacggt
     2761 tcccgcgaag gggcgagcaa ccgggtgatg ggcggcgcgg tgggcgacag cgtggcgggc
     2821 gcgatcggca agatgaaggg ttggctttcc cggaatttct agattcgcgg cccgttcccg
     2881 gacggaacgg acccgacggc gccgcggcgc cacggctcac gaggagccga acctttcgaa
     2941 acgaaaggca ttacgacagc agcacgacaa cagcagcacg gaggacgggc aatgttcgaa
     3001 ctggtggaaa aactcgcacc caatgcggtg atcaaggtga tcggcgtggg cggcggcggc
     3061 ggcaatgccg tgggccacat ggtcaattcg acaatcgacg gcgtggattt cat
//
LOCUS       DASSNH010000314         8781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1674346, whole genome shotgun sequence.
ACCESSION   DASSNH010000314 DASSNH010000000
VERSION     DASSNH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674346"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /gene="ligD"
                     /locus_tag="VFK00_11775"
     CDS             <1..1701
                     /gene="ligD"
                     /locus_tag="VFK00_11775"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET7593666.1"
                     /translation="RAPEIVDAIGDLGLKSARLDGELIALDNGLSDFNLLQKTLSGEA
                     SAPLVYMLFDLPYLDGYDLSRTPLVERKELLEKLVGQGRARLGFSSHWVGEGPRVFEM
                     ATQQKLEGIISKRVDSPYRGGRGDDWLKIKRLMSDEFAVVGYTPPKGQRTGFGSLLLA
                     ARRDDGTWRYAGRVGTGFNDQMLREVARDLATGGQKQPLVPIEGIDPLLRDARWVEPR
                     AVAEVYYRGIGNQGLLRQPSLKTLREDKSPEDLRDSDRSPSPASKAPKGKRARAPARE
                     EVHITHPDRVVYPDNGVTKQDVADYYAAVMEHFLPGVLERPVSVIRCPSGIEGQCFFQ
                     KHELAGLKRIGTVKLKEESGAQAIYLYPKDADGVMELVQFGVLEFHPWGATVRDPERA
                     DRIVFDLDPGEDVDWSRVVAAARLVRKLLEQAGLESFLRTTGGKGLHVVLPLDPPCPW
                     ALVKDFTQGFAKALAELHPQEFVAVSTKRIRRGRIFIDYLRNSRGATSIASYSLRARK
                     GAPVAVPLRWSELGKLESGAAYDIHSARQRLARQRKDPWEGFDNVKQDLKKIMQGSGG
                     "
     gene            complement(1887..2072)
                     /locus_tag="VFK00_11780"
     CDS             complement(1887..2072)
                     /locus_tag="VFK00_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593667.1"
                     /translation="MSDDSKDTKRPNHQKPRKESPAERRERKGHESDNQDEALEETFP
                     ASDPVSPFVPAKPPAED"
     gene            complement(2309..2743)
                     /locus_tag="VFK00_11785"
     CDS             complement(2309..2743)
                     /locus_tag="VFK00_11785"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7593668.1"
                     /translation="MAGGTTGFAAEAARGGMLEVRLADLALQKSNDADVKSFANRMKT
                     DHTAANEKLKSVVGSNEQLPTTLSAEQQQQVSSLESKSGKDFDKAYADTMVQDHQKDV
                     SEFENAQNNAPTQDLRQFASQTLPTLKEHLKLAQQLQSKVGS"
     gene            2742..3083
                     /locus_tag="VFK00_11790"
     CDS             2742..3083
                     /locus_tag="VFK00_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593669.1"
                     /translation="MGEVDAVAGIAGAMVVAPGAIALLAPLAGLYIGEPADVVAAGAA
                     GMGAVLAPSAGLLLLLPPEQPAAVAIATTSADGRARRCMRGRDMVGSFRWSVVIDYSS
                     LQPATAPVNGQ"
     gene            complement(3153..3467)
                     /locus_tag="VFK00_11795"
     CDS             complement(3153..3467)
                     /locus_tag="VFK00_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593670.1"
                     /translation="MMMMTDGDLCTLRFRFDGEGEQVATLVAMMRSMDNVDRVVEVGD
                     ETAHMREDSSSAGLSDDDVRADFHDVEVHAIDRDAAEQARDLVEIAARDLGVVVEYVD
                     RF"
     gene            complement(3475..4476)
                     /locus_tag="VFK00_11800"
     CDS             complement(3475..4476)
                     /locus_tag="VFK00_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7593671.1"
                     /translation="MPGKKKAHAATRSTAGAVRKQREIQSRMDRRDEEKGSKSAKKKP
                     AVQAGVRKQPVDLPAQHLAKPGREHELEEAPRYLAPDYRGSGKLEGRVALITGGDSGI
                     GRAVAVLFAREGADVAIVYLSEDEDARETAEAVENEGHSCLLIPGDVKDPAFCAEAVD
                     RTLEEFGHLDILVNNAAFQEHSERLEDISEEHLQETLQTNIGGYFHMARAAVPHMQEG
                     SAIINTGSETGLFGNPMLLDYSATKGAIHAFTRSLAKNLKDRGIRVNAVAPGPVWTPL
                     NPADRPAKKMAKFGKDSAMGRAAQPEELSPAYVFLAAPVCSSYISGSVLEVLGGPTG"
     gene            5077..5832
                     /locus_tag="VFK00_11805"
     CDS             5077..5832
                     /locus_tag="VFK00_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7593672.1"
                     /translation="MSLPAAPVAPAIRRRPAGMSRRVACGNRLIDALPRSARARLLAA
                     GKRVDLRPGLVLCEPGQRIRHAWFPLEGFLGLSTHPSHPARMEVDLVGNEGMLGAALV
                     LGVAEASQRALVLGAGVALRIDAATLRRELQRVRALRRLLNRYVYVLMMQFLQNSTCV
                     ASHKIEQRLARLLLMSQDRARAEALNITQASLAEMLGVRRVGVTEAAGALQRLGYIRY
                     SRGRVAVTDRAGLERAACGCYRAVRASHQRWLG"
     gene            6006..6398
                     /locus_tag="VFK00_11810"
     CDS             6006..6398
                     /locus_tag="VFK00_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593673.1"
                     /translation="MEVIPSNLCTLVEQLEDARQFHEGAALRSAGFQRECHSERSRAY
                     ATMLSEIRDWESRKRRPSNGPDFHAAVDAPGDILTDDSYAALLERVYRIHSSVASHRR
                     AIGLTAGAVAMGNMAAHGRPREGEPMHC"
     gene            6450..6836
                     /locus_tag="VFK00_11815"
     CDS             6450..6836
                     /locus_tag="VFK00_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593674.1"
                     /translation="MRMEKNRVSRRRVGASDLEFGLEGCPHGVDRENVLDLITVIEDE
                     HRFHAAAAVMARDPAERERCLAMAHDAAALLANIRHGLASATRGRLSGRERARRMEQY
                     AYRLMVRTQRPRHRVLSSTAHLQGAE"
     gene            6836..7351
                     /locus_tag="VFK00_11820"
     CDS             6836..7351
                     /locus_tag="VFK00_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593675.1"
                     /translation="MNAAERAVVRKLVADLDNTRLLHTTLAPLLIRPHLKFLIERMAR
                     AHATIADDLIGQMGGTGSAARERGASRFARMRVRIERRIAITNLDIELGCLKCIARHE
                     GRVTQRLRRALENVRPLQQSLHRELGQLERLRLRIESLMHEMEMPPLAAAMPRHRADV
                     MPVPSHGRHRP"
     gene            7348..7539
                     /locus_tag="VFK00_11825"
     CDS             7348..7539
                     /locus_tag="VFK00_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7593676.1"
                     /translation="MNDEKLKWQWKHLGAGLKARWHKLTDDDVQVAVGNAEYLVRLLQ
                     ERYGIAREEATRQIRKFEP"
     gene            7950..8357
                     /locus_tag="VFK00_11830"
     CDS             7950..8357
                     /locus_tag="VFK00_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7593677.1"
                     /translation="MKTRIAAAVASLLVASMGTAAYAQGAQQTTPQTTPPTSTPYTSN
                     GTQATQPMYNNDSNMSASRTEQQIKQKLTANGITATNVNITFNDGTATLSGTVATQQD
                     IAKARMEAMRVQGVKHVDTSNLQVRADNGSMQH"
     gene            8673..>8781
                     /locus_tag="VFK00_11835"
     CDS             8673..>8781
                     /locus_tag="VFK00_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HET7593678.1"
                     /translation="MLYYAVVFLIIALIAGFFGFFGVAGLAATIAKILFV"
BASE COUNT         1433 a         2914 c         2943 g         1491 t
ORIGIN      
        1 cgggcgccgg aaatcgtgga cgcgatcggc gacctcggat tgaagtcggc ccggctggac
       61 ggcgaactga tcgcgctgga caacggcttg agtgacttca acctgttgca gaagacgctg
      121 tccggcgagg ccagcgcgcc gctggtgtac atgctgttcg acctgcccta cctggacggc
      181 tacgacctgt cgcgcacgcc cctggtcgag cgcaaggagc tcctcgagaa actggtgggc
      241 caaggccggg cccgcctcgg cttcagctcc cactgggtgg gcgaaggccc ccgcgtgttc
      301 gagatggcga cccagcagaa gctggaagga atcatctcga agcgcgtgga ttcgccttat
      361 cgcggtgggc gcggcgacga ctggctcaag atcaagcgcc tgatgagcga cgagttcgcg
      421 gtggtcggtt ataccccgcc gaagggccag cgcactggct tcggctcctt gctgctcgcg
      481 gcgaggaggg acgatggcac ttggcgctac gccggccgcg tcgggaccgg attcaacgac
      541 cagatgctgc gcgaagtggc ccgcgacctc gccaccggcg gccagaagca gccactggtg
      601 ccgatcgagg gcatcgatcc gctgttgcgc gacgcgcgct gggtggaacc gcgggcggtg
      661 gccgaggtgt actaccgtgg catcggcaac caggggctcc tgaggcaacc ctccttgaaa
      721 acgctgcgcg aagacaagtc gccggaagat ctccgcgatt ccgaccgttc cccctccccc
      781 gccagcaagg cgcccaaggg caagcgcgcc cgtgcgccgg cgcgcgagga agtccacatc
      841 acacatccgg accgcgtcgt ctatcccgac aatggcgtga ccaagcagga cgtggccgac
      901 tattacgctg cggtcatgga acatttcctg cccggggtgc tggagcggcc ggtgtccgtg
      961 atccgctgcc cgtcgggaat cgaagggcaa tgcttcttcc agaagcacga gctggccggt
     1021 ttgaaacgca tcggcaccgt gaagctcaag gaagagtccg gcgcgcaggc aatctacctg
     1081 tatccgaagg atgccgatgg cgtgatggaa ctggtgcagt tcggcgtcct ggaattccat
     1141 ccctggggcg cgacggtgcg cgatccggag cgcgcggacc gcatcgtgtt cgacctcgac
     1201 cccggcgagg acgtggactg gtcgcgcgtg gtggccgcag cgcggctggt gcgcaagctg
     1261 ctggagcagg caggcctcga gtcgttcctg cgcaccaccg gcggcaaggg gctgcacgtc
     1321 gtgctgccgc tcgacccgcc ctgcccttgg gcgctggtca aggatttcac ccaagggttc
     1381 gcgaaggcgc tcgcggaact gcatccgcag gaattcgttg ccgtgtccac caagcggatt
     1441 cggcgcggac gcatcttcat cgactacctg cgcaacagcc gcggcgcgac cagcatcgcg
     1501 agctattcac tgcgcgcgcg caagggcgcg ccggtcgcgg tgccgctgcg ctggtccgaa
     1561 ctcggcaagc tcgaaagcgg tgccgcctac gacatccact cggcacgcca gcggctcgca
     1621 cgacagcgca aggatccctg ggaaggcttc gacaacgtca agcaggatct gaaaaagatc
     1681 atgcaagggt ccggcggctg acggcgtcgc cggaacgctt cggaagccat gtaggagcgc
     1741 gccttgcgcg cgatgcgttc cctcggaagg tcgctaaaac aagcgcctgg cgcaagcctg
     1801 cttcctgcct gctgaaagcg gcggtcgcgg gcgggcccgc tcctacgaat tcacggcggc
     1861 ttccgcgcga ccgtgaccat gccggttcag tcttccgccg gcggctttgc ggggacgaat
     1921 ggcgaaaccg gatcgctggc gggaaaggtt tcctcgagcg cttcgtcctg gttgtcgctt
     1981 tcgtggccct tgcgctcccg tcgctccgcg ggcgattcct tccgcggttt ctggtggttg
     2041 gggcgcttgg tgtctttgct gtcgtcggac atggggcgta ctccggcgcg ggtggagtat
     2101 gagtgtccgc cgggccgggt gaacgcacgc gtagcggtgc gcactgcgcg aacagcgctt
     2161 ctcgggaggc tgggctttgc ggccgcgaca aaaaaggagc gggcgcttcg cgcccgctcc
     2221 acgttgcatt cggtcgcggg catggcccgc tcctgcaaca acgacaattc atggggagcg
     2281 ggacattgcc cgctcctgca aaccggcgtc agctaccgac tttcgactgc agttgctgcg
     2341 cgagcttcag gtgctccttg agcgtgggca acgtctggga tgcgaactgg cgcaggtcct
     2401 gcgtcggcgc gttgttctgg gcgttctcga actcggagac atccttttga tggtcctgaa
     2461 ccatcgtgtc tgcatacgcc ttgtcgaagt ccttgccgct cttggattcg agcgaactta
     2521 cctgttgctg ctgctcggcg ctcagggtgg tcggcaattg ctcgttcgac ccgacgaccg
     2581 acttcaactt ttcgttggcg gccgtatggt ccgtcttcat ccggttcgcg aacgacttga
     2641 cgtccgcatc gttgctcttc tgaagcgcaa ggtcggcgag cctgacttcc agcattcctc
     2701 cgcgcgcagc ctccgcggcg aagccggtgg tgccgccggc catgggggaa gtcgacgcgg
     2761 tggccggcat tgccggggcc atggtggtgg cgccgggtgc catcgcgttg ctggcgccgt
     2821 tggccgggtt gtacatcggg gaaccggccg acgtggtggc cgcgggcgcg gccggcatgg
     2881 gggcggtact ggcgccttcc gcgggattat tgttgttgtt gccaccggag caacccgcag
     2941 ccgtggcgat cgcgacgacg agcgcggatg ggagggcgag gcgttgcatg aggggtcgtg
     3001 acatggtggg ttccttccgc tggtcagtgg tcatcgatta cagcagcctg cagcctgcca
     3061 ccgcccccgt gaacggccag tgatcgaggc gcgaatgggc ggtttcgtgc aaccatcgcc
     3121 gttgcgcaac ggttcatgaa accggacaca gctcagaagc ggtcgacata ttccacgacc
     3181 acgccgaggt cgcgcgccgc gatttcgacc aggtcgcggg cctgctcggc cgcgtcgcgg
     3241 tcgatcgcat ggacttccac gtcgtggaag tcggcgcgga cgtcgtcgtc ggacagcccc
     3301 gcggaactcg agtcctcccg catgtgggcc gtctcgtcgc cgacctccac cacccggtcg
     3361 acattgtcca tcgagcgcat catcgcgacc agcgtcgcca cctgctcgcc ttcgccatcg
     3421 aaccggaaac gaagggtgca caagtctcca tcggtcatca tcatcatggc attctcatcc
     3481 tgtcgggcca cccagtactt ccagcacgct gccgctgatg taggacgaac agaccggggc
     3541 ggcgaggaac acgtaggcgg gggataactc ttcgggttgc gcggcgcgac ccatggcgct
     3601 gtccttgccg aacttcgcca tcttcttggc gggacgatcg gcgggattca acggcgtcca
     3661 tacgggacca ggtgccaccg cattgacgcg gataccgcgg tccttgaggt tcttggcgag
     3721 ggaccgagtg aaggcgtgga tcgcgccctt ggtcgccgag tagtccagca gcatgggatt
     3781 gccgaacagc cctgtttccg aaccggtgtt gatgatcgcg ctgccctcct gcatgtgcgg
     3841 caccgcggcg cgcgccatgt ggaagtagcc accgatgttg gtttgcaggg tttcctggag
     3901 gtgttcttcg ctgatgtcct cgaggcgctc gctgtgctcc tggaaggcag cattgttcac
     3961 gaggatgtcg agatggccga attcctccag ggtgcggtcg accgcttccg cgcagaaggc
     4021 gggatccttg acgtcgcccg ggatcaggag gcaagagtgc ccctcgtttt cgaccgcctc
     4081 ggcagtctcg cgggcgtcct cgtcttcgct gagatagacg atcgcgacgt ccgcgccctc
     4141 gcgggcgaac agcaccgcga ccgcgcgccc gatccccgag tcgccacctg tgatcagcgc
     4201 gacccgtccc tccagcttgc cgctgccgcg gtagtccggg gccaggtaac gcggcgcctc
     4261 ttcgagttcg tgctcgcggc cgggtttggc gagatgctgc gccggcaggt cgaccggctg
     4321 cttgcgcacg cccgcctgca cggcaggctt cttttttgcg gatttggagc ccttctcctc
     4381 gtcgcgccga tccatgcggc tctggatctc gcgttgcttg cgtacggcgc ctgccgtgct
     4441 tcgtgtggcg gcatgcgcct ttttcttccc tggcatggat ttctccttgt cgctgatccg
     4501 gcggaccgcg tcggcaaata ctcgctggcc gaaagtatcc tccgcgtcaa aagtggggtc
     4561 attccggaga atgcgacagc ccgccgttgg caccggttca cgtggggcgc caagcggatg
     4621 cggcggcgtt gctgatccag atggccagac cgccgtttgc aaccgccacc acgcgatcga
     4681 ccggcagttg tgccgctggc tgtcgatgcg gttggcccgg ctgccgtcga accagctcgt
     4741 gatgacggag gagctgatcg cgcgcatgct cggggtgcct cgctaggcgt caccgaagcg
     4801 gccggcaggc tgcggcgcgc gggcgcgagc cattacaagc gcgggtggat cgagctgcgg
     4861 gaccggcgca agctggaatc catcgcctgc gaatgctgtg gcgtggtgcg ctcggagttc
     4921 gcgcgcccgt tgccatggaa ggcggagtgg aacgcgggcg gagccgcgat gtccatggct
     4981 gtgggacgca tcgcggtttt tccccggcct ctggcacgca gcgtgcatcg ccccccgcca
     5041 ggaatgcagc cgcgtcctcc tgagcctcga gatcccatgt ccttgcccgc agcacccgtt
     5101 gctcccgcga tccgccgccg gccggcgggg atgtcgcgcc gggttgcctg cgggaatcgc
     5161 ctgatcgatg cgctgccccg cagcgcgcgc gcacgcctgc tcgcggccgg caagcgcgtg
     5221 gatctgcgcc ccggcctcgt cctttgcgag cccggccagc gcatccgcca cgcgtggttc
     5281 ccgctcgagg gcttcctggg cctgtcgacg catccgtcgc accctgcgcg catggaagtc
     5341 gacctggtgg gcaacgaagg gatgctgggc gccgcgctcg tactgggcgt cgccgaagcc
     5401 agccagcgcg cgctggtgct gggcgccggc gtggcattgc ggatcgatgc ggcgacgctg
     5461 cggcgggagt tgcagcgcgt ccgcgcgttg cggcgcctgc tcaaccgcta tgtctacgtg
     5521 ttgatgatgc agttcctgca gaactccacc tgcgtcgctt cgcacaagat cgagcaacgc
     5581 ctcgcacggc tgttgctgat gtcgcaggac cgggcgaggg ccgaggcctt gaacatcacc
     5641 caggcatccc tcgccgaaat gctgggcgta cgccgggtgg gcgtcaccga agcggccggg
     5701 gccctgcagc ggctcgggta catccgctat tcgcgcggac gcgtcgcggt gacggaccgt
     5761 gcgggcctgg aacgcgccgc ctgcggctgc taccgggctg tccgagccag ccaccagcgc
     5821 tggctcggct gacgctcgga cgtccatacg tccattgcgg atgcgccgcc gcgcgtcgcc
     5881 ggcgccttaa cgttttgttg acggcatttg tccgaggatc tccttctccg caatgtgcga
     5941 ctccgtacat acgcactgcg ggcgaacgtg ggagcctgtg ttccccttgg caatggaagc
     6001 catccgtgga agtgattccc tccaacctgt gcaccttggt cgagcagctc gaggacgcgc
     6061 ggcagttcca cgagggtgct gcactccgat cggccggatt ccagcgcgag tgccacagcg
     6121 agcggtcgcg cgcctatgcc accatgcttt cggagatcag ggattgggag tcccggaagc
     6181 gccgcccctc caacggaccg gacttccacg ccgccgttga cgcgcccggc gacatcctga
     6241 cagacgacag ttacgccgcg ctgctggagc gggtctaccg catccacagc tcggtcgcgt
     6301 cccatcggcg agccatcggc ctcactgcag gcgcggtcgc catgggcaac atggccgcgc
     6361 atggccgccc gcgtgaaggc gaacccatgc actgttgagc cacttgaacg ggggcaaccg
     6421 ctggaccggt gatgcaagga gaagcagccg tgaggatgga gaagaaccgg gtttcgcgaa
     6481 ggagggtcgg agcatcggac ctggagttcg gcctggaggg ttgtccgcac ggcgtggacc
     6541 gcgaaaacgt cctcgacctg atcacggtga tcgaggacga gcatcgcttc catgccgcgg
     6601 ccgcggtgat ggcccgggat cccgcagagc gcgagcgctg tctggccatg gcgcacgacg
     6661 ccgccgcgct cctggcgaat atccggcacg gcctcgcaag cgcgacccgc ggccgccttt
     6721 ccggccgcga gcgggcacgc cggatggagc agtacgcgta ccgcctgatg gtccggaccc
     6781 aacgaccgcg ccaccgcgtg ctgtcgtcga cggcgcacct gcagggggcc gaataatgaa
     6841 cgccgctgaa cgtgccgtgg tccggaagct ggtcgccgac ctcgacaaca cgcgcctgct
     6901 gcacacgacc ctcgcgcccc ttttgatccg cccgcacctg aagttcctca tcgagcgcat
     6961 ggcccgtgcg cacgccacca tcgcggatga cctgatcggc cagatgggcg ggaccgggtc
     7021 ggccgcccgg gagcgcggcg ccagccggtt tgcgaggatg cgggtgcgga tcgagcgccg
     7081 gatcgcgatc accaacctcg acatcgagct gggttgcctg aagtgcatcg cgcgccacga
     7141 gggccgcgtc acgcaacggc tgcgacgcgc gctggagaac gtccggccgc tgcagcagag
     7201 cctgcaccgc gaactgggcc agctcgagcg cttgcggttg cggatcgaat cgctgatgca
     7261 tgaaatggag atgccgcccc tggcggcggc gatgccccgc caccgcgcgg acgtgatgcc
     7321 cgtgccgagc cacggtaggc acaggccgtg aacgacgaga agctcaagtg gcagtggaag
     7381 catctcggcg ccggcctgaa ggcccgttgg cacaagctca ccgacgatga cgtgcaggtt
     7441 gcggtcggca acgccgagta tctggtccgc ttgctgcagg agcgctatgg catcgcccgc
     7501 gaagaggcca ccaggcagat caggaaattc gaaccctgat cgatcgcggc tgtcgatggc
     7561 cgctcccacc tgttttcgac ggcgcatgga ggcgccgaag tgccgccccg gcgcacgttc
     7621 ccactcgacg ccgaacggca gcaggatccc gggatcccca tcacttcgaa ccggttccat
     7681 ggacggacgg ttcgaagacc attcactttt gcggcctctg gttcgaagga cattcagcgc
     7741 gggcgcccac aatggaaccc agcatgagca cccggcaaga cccatcgcaa caccaaccag
     7801 aacgccttcc tccgcgccgt cagcgcgatc cgtccaacga caacgacatc cccgcgcgcc
     7861 gggatccgaa acgcgagggc catcgcgggg acgcgcggcg cacggaccgc aggcgttgat
     7921 ccatgaccac caccgacaag gagtcaatca tgaaaacccg aattgcagcc gccgtcgcat
     7981 ccctgctcgt cgcatcgatg ggcaccgcag cgtacgccca gggcgcgcag cagacgacgc
     8041 cacagaccac tccgccgacg tccaccccgt ataccagcaa cggcacccag gccacccagc
     8101 cgatgtacaa caacgacagc aacatgtcgg ccagcaggac cgagcagcag atcaagcaga
     8161 agctgactgc caatggcatc acggcgacca atgtcaacat tacgttcaac gacggcaccg
     8221 caaccctcag cggcaccgtg gccacccagc aggacatcgc caaggcacgc atggaagcga
     8281 tgcgggtcca gggcgtcaag cacgtggata cgtcgaacct gcaggtgcgc gccgacaatg
     8341 gctcgatgca gcactgatgg accctcgcgc cgcagcggcc ggtgagcatc cggccgccgg
     8401 cgaggaggcg gaggatgccg cctgatcggt ttcgctccgc gcgacgcagg acgcgtcgcg
     8461 aatggcggct cggtacgcgc gaggaaacaa gcgtgccgag gccgccgaac gaatcggctt
     8521 ggtctccctg tggcgccgat tcgattgatt ccgggcaagc cagggatggc gcgcccggag
     8581 tttttttacc gctgccgcga actcctcaat cggacgcacc accgacccgc ttgcggatct
     8641 caacctgcac ctgaaaggaa aggagtacca ccatgctcta ctacgcggtc gtgttcctga
     8701 tcatcgcgct gatcgccggg ttcttcggat tcttcggtgt cgccggcctc gccgccacca
     8761 tcgccaagat cctgttcgtc a
//
LOCUS       DASSNH010000315         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_897530, whole genome shotgun sequence.
ACCESSION   DASSNH010000315 DASSNH010000000
VERSION     DASSNH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897530"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VFK00_11840"
     CDS             <1..1588
                     /locus_tag="VFK00_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7593679.1"
                     /translation="ANLGVVLLMFTLGLDFSLRKLRALGAGVMLAALVEVAVMLWLGI
                     EIGRLAGLNGREALFLGGVICFSSTMIALRTVADRGWRGQGFVAGMVGMLVSEEVLTV
                     VLLTVLSAIAAGSSNTAGIAFTVLWRMLLFVSVALVLGLLLLPRLLDWLARRGRDEPL
                     LVTSLGICFGACLLAEWEGFSVALGAYLAGVVVAEARCSERIVRLVRPVRDMFAAIFF
                     VAIGLLLDPTQLLPWLWPALGLALAVIVGKTVACSVGAFVCAGRGVRDSLRTGLTMAQ
                     IGEFSFVIASLGMAAGGAGKFLYPVTVTAAVVCMLVSPMLLRAEPLLIGGLRRMTPGP
                     LRSLAVGYHEWMGGTAAAERDQMLARMLRRLLWHIAVNLILVCAVFAIGAFVSAMVPR
                     WWPHLPLSPVVRRSLVWAGALFVSLPMLIAVYRKADALGMLLAEIGISEQVHGVRTAG
                     LRRVLGKLVPLGTLAGMALLVSVLGSSILPPRGVLIALLAIGAFTTWFLWGSLVRVHA
                     RMQATLRDLIEQDSASLPRE"
     gene            complement(1764..2567)
                     /locus_tag="VFK00_11845"
     CDS             complement(1764..2567)
                     /locus_tag="VFK00_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593680.1"
                     /translation="MRILAILACVAVAVLAIPPAHAETTVELVATWPAGETVTLSRNQ
                     TFYLHLRYTSDQPVQIWARPYFQGKPAHAGSNPSRTYPPGSGEALGWFFLDPGAVVDE
                     VRIRAGDSSPNGTHVVASYRVAVSGGDASTQAGAPPAWVTTLRAADKAAQDSAYQEAM
                     NRPVSAGETLFFTGFMLLMAGLGLFGLLAPAWGLWRWRGGWRIAAAAPAVVMGFVVLR
                     ILVGTSIDPTSHNLWPFEILMVGAASTVFMIGLMLARKVTGAGRGLAAR"
     gene            2888..3577
                     /locus_tag="VFK00_11850"
     CDS             2888..3577
                     /locus_tag="VFK00_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7593681.1"
                     /translation="MRGAKIASNRPTVAKKAMQESREMRLRFPLLSSMNIKFGMAAAL
                     LGVFGLIAAPLANATPISFYNDAGNQDTLRFTLGCGSSDGSCSGLIEVLESESPTSWG
                     DGPGDVFDLGNSGDATELGFVNTVTGENFTAGMKLDGNGGSESFSISGEYVLIKIGNK
                     YGLSYAFIHNLGGSLDVFLSAMPGQGAGLSHYTQFGTPTVGVPEPAELGMLGFGLLLI
                     GGFVGLRRRYH"
     gene            complement(3710..>3979)
                     /locus_tag="VFK00_11855"
     CDS             complement(3710..>3979)
                     /locus_tag="VFK00_11855"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET7593682.1"
                     /translation="WPGQALSYMIGEQVILQLRDEARQKLGDKYNLKHFDDVVLGQGS
                     LPMAILQEVVDKWIDNTLAGKPADQLPFACKPRSLEVCTADDGSR"
BASE COUNT          590 a         1247 c         1386 g          756 t
ORIGIN      
        1 cgcgaacctc ggcgtggtgc tgctgatgtt caccctgggg ctcgacttca gcctgcgcaa
       61 gctgcgcgcg ctcggcgccg gagtgatgct cgccgcgctg gtggaagtcg cggtgatgct
      121 gtggctgggc atcgagatcg ggcgcctcgc cggattgaac ggtcgcgagg cgctgttcct
      181 gggaggcgtg atctgctttt cctcgacgat gatcgcgttg cgcacggttg ccgaccgcgg
      241 ctggcgcggg cagggcttcg tggcgggcat ggtcggaatg ctggtgtcgg aagaggtgtt
      301 gaccgtggtg ctgctgaccg ttctcagcgc gatcgcggcc ggcagttcca ataccgccgg
      361 cattgcgttc acggtgctgt ggcggatgct gctgttcgtg tcggttgcgc tggttttggg
      421 cttgctgctg ctgccgcggc tcctcgactg gctggcgcga cgcggccgcg acgagccgct
      481 gctggtgacc tcgctcggca tctgtttcgg cgcctgcctg ctggcggagt gggagggctt
      541 cagcgtcgcg ctgggcgcgt acctcgccgg cgtagtggtc gccgaagcgc gttgctcgga
      601 gcgcatcgtg cggctggtgc ggccggtgcg cgacatgttc gcggcgatct tcttcgtggc
      661 gatcgggctg ttgctggatc ccacgcagtt gctgccgtgg ctgtggccgg cactggggct
      721 cgcgctggcg gtgatcgtcg gcaagaccgt cgcgtgcagc gtcggcgcgt tcgtgtgcgc
      781 gggccgcggc gttcgcgatt cgctgcggac cggattgacc atggcgcaga tcggcgagtt
      841 ctcgttcgtg atcgcttccc tcggcatggc ggccggcggg gccggcaagt tcctgtatcc
      901 cgtgacggtg accgcggcgg tggtgtgcat gctggtgtcg ccgatgctgc tgcgcgccga
      961 gccgttgctg atcggcggct tgcgccgaat gacgcctggc ccgctgcgct ccctggcggt
     1021 tggctaccac gaatggatgg gcggcaccgc tgcggcagag cgtgaccaga tgctggcgag
     1081 gatgctgcgg cggctgctct ggcacatcgc cgtgaacctg atcctcgtgt gcgccgtgtt
     1141 cgcaatcggc gcgttcgtca gcgcgatggt gccgcgctgg tggccgcacc tgccgctgtc
     1201 gccggtggtg cggcgttcgc tggtctgggc gggggcgctg ttcgtgtcgc tgccgatgct
     1261 gatcgcggtc tatcgcaagg ccgatgcgct gggcatgttg ctggccgaga tcggcatcag
     1321 cgagcaggtc cacggcgtgc gcaccgcggg cctgcgccgg gtgctgggca agctggtgcc
     1381 gctcggcacc ctcgcgggca tggccctgct ggtcagcgta ctcggaagca gcatcctgcc
     1441 gccgcggggc gtgctgatcg cattgctggc gattggcgcg ttcaccacgt ggttcctgtg
     1501 gggctcgctg gtgcgggtgc acgcccgcat gcaggcgacg ctgcgcgacc tgatcgaaca
     1561 ggactcggcg tcgttgccgc gcgaatgagc ttggcccgac aaccgtcgaa ggccgttggg
     1621 cttcgctgcg ctcagcgcca acctacaaag gcgcgcggtg caacggtgag tcggttcgca
     1681 ggttgggttg atcccggaac gtgttccggg agaacccaaa caagcgccat gtagatggcg
     1741 ctcggcgcaa cggggtcctc gcgtcaacgc gccgccaggc cgcggcccgc gccggtgacc
     1801 ttgcgcgcca acatgagccc gatcatgaac acggtgctgg ccgcgccgac catgaggatt
     1861 tcgaacggcc acaggttgtg cgaggtcgga tcgatcgagg taccgacgag gatgcgcagc
     1921 accacgaatc ccatcacgac ggcgggcgct gcggctgcaa tccgccaccc gccgcgccag
     1981 cgccacaagc cccacgccgg cgcaagcaaa ccgaacagcc cgagccccgc catcaacagc
     2041 atgaaaccgg tgaagaacag cgtctcaccc gcgctgacgg gccggttcat ggcttcctgg
     2101 taagccgagt cctgcgcggc cttgtcggcc gcgcgcagcg tggtcaccca ggcaggtggt
     2161 gcgcccgctt gggtggacgc gtcgccgccg ctcactgcaa cccgatagct ggccacgacg
     2221 tgcgtcccgt tcggcgagct gtcgccggcg cggatccgca cttcgtcaac cacggcaccg
     2281 ggatccagga agaaccaacc cagcgcttcg ccgctgcccg gaggataggt gcgcgaggga
     2341 ttgctccccg catgcgccgg cttcccctgg aagtacggac gggcccagat ttgcaccggc
     2401 tggtcgctgg tgtagcgtaa gtgcagatag aaggtctggt tgcggctgag cgtcacggtt
     2461 tcgccggctg gccaggtagc cacgagttcg acggtggttt cggcgtgcgc gggcgggatc
     2521 gccagcacgg ccacggccac gcaggccagg attgccagga tgcgcatcac ggatctcccc
     2581 ggtccggttg cccgccgcga gcataccaag cgaggccggg atggattggg tgcccgattc
     2641 aggttttcag gcggagcggt ttggctcccg taccgcagct tccgcggtat cggcaggtgc
     2701 cgttgtctgg cgcgtcgccg ggacttacat ggggccccgg caggctacct tgaaaaatac
     2761 gtccagaaac aacgagtttg gccacatgtc cacgccgatc cctgcagccc ctgtcgggca
     2821 accttacaaa agtgccattt tcgggacaat cagcctggaa taaaagcctt tcatgacagg
     2881 ggcttgtgtg cgtggcgcga aaattgcttc gaataggcca acggtcgcga aaaaagcgat
     2941 gcaggagagc cgagaaatgc gcctacgttt tccgctgttg tcgagcatga acatcaagtt
     3001 cggaatggcc gccgccttgc tcggagtgtt cgggctgatt gcggcaccct tggcgaacgc
     3061 gaccccgata tcgttctaca acgacgccgg caaccaggac acgctgcgtt tcacgctggg
     3121 atgcggcagt tcggacggaa gctgctccgg cctgatcgag gtgcttgaat ccgaatcgcc
     3181 gacctcgtgg ggtgacggcc cgggcgacgt gttcgacctg ggcaattccg gcgatgccac
     3241 cgaactgggt ttcgtcaata ccgtcacggg tgagaacttc acggccggaa tgaagctgga
     3301 cggaaacggc ggcagcgaaa gtttctccat ttccggagag tacgttctca tcaagatcgg
     3361 caacaaatac ggtttgagct acgcttttat ccacaatctc ggcggctcgc ttgatgtgtt
     3421 tttgtctgcc atgccgggac agggcgcggg actcagccat tacacccagt tcggaacgcc
     3481 gacggtcggc gtccccgagc ctgcggaatt ggggatgctc ggcttcggcc tgctgctgat
     3541 cggcggattc gtcgggctgc ggcgcaggta ccactgaccg tacgtccttt ccagtgtctt
     3601 tttttccagc agtccccggc aacggggact gcctgtttat gggccacgtc gggcacgcgc
     3661 gcggacgaga ggctatcgcc cgcaagcacc ggggctttcc gggcgagcgt catcgcgaac
     3721 catcgtcggc cgtgcagact tccagactgc gcggcttgca cgcgaacggc aactggtcgg
     3781 ccggcttgcc tgccagggtg ttgtcgatcc atttgtccac cacttcctgc aggatcgcca
     3841 tcggcaggct gccctggccc agcaccacgt cgtcgaaatg cttgaggttg tacttgtcgc
     3901 cgagcttctg cctggcctcg tcgcgcaact gcaggatcac ctgctcgccg atcatgtacg
     3961 acagcgcctg gccgggcca
//
LOCUS       DASSNH010000316         8717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1804791, whole genome shotgun sequence.
ACCESSION   DASSNH010000316 DASSNH010000000
VERSION     DASSNH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804791"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFK00_11860"
     CDS             <1..565
                     /locus_tag="VFK00_11860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7593683.1"
                     /translation="VRRGELDARQRIEAAAGVLHSLEKLPEFMTDRRVAGYWAVRGEL
                     PLNLAVASLARREQRYFLPVLGESRQLRFGEYRDGTRLDSNRFGIPEPDVPTEDLHGP
                     ADMDVILLPLLAFDRLGRRLGTGGGWYDTSLAHLREVPRPATPLLVGVGYAFQEVEAV
                     PPEPWDVDVDYIATDGELIACRADGAT"
     gene            576..1025
                     /locus_tag="VFK00_11865"
     CDS             576..1025
                     /locus_tag="VFK00_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET7593684.1"
                     /translation="MRRWLMKTEPDTFSIDDLKQRRVEPWNGVRNYQARNFIREMTRG
                     DEVMIYHSSCAVPGVAGLAKVASAPYPDPGQFDPKDDYFDAGSDRGNPRWSLVDVRFV
                     RKFKRVVTLDEIKQLEGLGDFALTRRGNRLSILPVSDAQWRAILELE"
     gene            1022..1681
                     /gene="rpiA"
                     /locus_tag="VFK00_11870"
     CDS             1022..1681
                     /gene="rpiA"
                     /locus_tag="VFK00_11870"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET7593685.1"
                     /translation="MKGGALDIEAQKRAAGEAALEYVEDNQVIGVGTGSTVRYFIEGL
                     ARMKHRLDGAVSSSEQSSALLREHGIQVLDPNAVGTIGVYVDGADECDPQRRLIKGGG
                     GALTREKILAAVAKQFVCIVDAGKCVEVLGKFAVPVEVIPMARSHVAREIQQRGGMPV
                     WRQGCVTDNGNWILDVHGLSITEPARVEAEWNQIAGVVTVGLFAQRPADVVLVGDGRR
                     L"
     gene            complement(1733..1807)
                     /locus_tag="VFK00_11875"
     tRNA            complement(1733..1807)
                     /locus_tag="VFK00_11875"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1773..1775),aa:Gln,seq:ctg)
     gene            complement(1981..2376)
                     /gene="rpsI"
                     /locus_tag="VFK00_11880"
     CDS             complement(1981..2376)
                     /gene="rpsI"
                     /locus_tag="VFK00_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7593686.1"
                     /translation="MATTQQNYGTGRRKTSAARVFLNKGSGQIMVNGRPLDEFFGRET
                     SRMIVRQPLELTQTADKFDIQVNVAGGGITGQAGAIRLGIARALVEYDETLKAPLRRA
                     GFMTRDARAVERKKVGLHKARRATQFSKR"
     gene            complement(2379..2807)
                     /gene="rplM"
                     /locus_tag="VFK00_11885"
     CDS             complement(2379..2807)
                     /gene="rplM"
                     /locus_tag="VFK00_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7593687.1"
                     /translation="MKTFSAKAESVKRDWLVVDASGKTLGRLCSEVARRLRGKHKPEF
                     TPHVDTGDYIVVVNAEKVAVTGNKLEDKNYHRFTGYVGNLKTTNLKDLLATHPERAIE
                     IGVKGMLPKNPLGRTMFRKLKVYRGPEHPHTAQQPKALEI"
     gene            3139..4335
                     /locus_tag="VFK00_11890"
     CDS             3139..4335
                     /locus_tag="VFK00_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7593688.1"
                     /translation="MSAPDARQHIIGLANACVKCGLCLPYCPTYALDRVETESPRGRI
                     ALSRHAVDGSVAPDADLRAHLDHCLGCLACERVCPVPVHYGELLTAYRAALPPASPRP
                     RRMLRLLTRPRWLRTGAALARWVGARHWLTPLARRLLPRHSALRAAVSLLPALPAAPR
                     AGTAVPAPAAARGKVALFGGCVQGALEREAQDAAACLLSAAGFEVRRMEGLCCGALAT
                     HTGDSVDAAAKARRVRDASAAESVDCVLTATPGCLGTLREDLPGTQVEDAMAFLDRQG
                     EALAFRPLERKAVLHLPCTQVNVARADGAVRRMLARIPGLELDVLPTRGMCCGAAGSY
                     MLEFPERAVAQRQRVFENLPDPLPGLLLTANIGCRLHLGAGLGERGTPMEVQHPLVLL
                     AQQLKA"
     gene            4332..4988
                     /gene="coq7"
                     /locus_tag="VFK00_11895"
     CDS             4332..4988
                     /gene="coq7"
                     /locus_tag="VFK00_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HET7593689.1"
                     /translation="MNRPTIDTRRLGPGDRVLAAFQRALDTVAGAAVAARPSPGEEHP
                     LPRLDPAARRHAAGLMRVNHTGEVCAQALYDGQAALARDSVTRAHLDAAAGEETDHLA
                     WCAQRLSELDDHPSLLNPVWYAGSFAIGALAAMAGDRWSLGFVVETERQVEAHLGEHL
                     ARLPPADARSRAILRTMQADEARHADNALARGGERVPPPIPALMRGASAVMKAVAYRV
                     "
     gene            complement(5103..5888)
                     /gene="speD"
                     /locus_tag="VFK00_11900"
     CDS             complement(5103..5888)
                     /gene="speD"
                     /locus_tag="VFK00_11900"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HET7593690.1"
                     /translation="MVKPLPRLKLQGFNNLTKALSFNIYDICYAVTNDQRDRYIDYID
                     EAYDADRLTQILTDVADIIGANILNIARQDYDPQGASVTILISEHPVIDKLGRDMISD
                     AVVAHMDKSHITVHTYPETHPHNGIATFRADIDVATCGVISPLKALNYLIDSFESDIV
                     TMDYRVRGFTRDIHGKKHYIDHKINSIQDYLAKHIRQKYEMFDVNVYQENMFHTKMHV
                     KDFDLDTYLFEAHAEDLSFRDRQKIESLLRREIEELFHGRNLM"
     gene            6090..6785
                     /gene="crp"
                     /locus_tag="VFK00_11905"
     CDS             6090..6785
                     /gene="crp"
                     /locus_tag="VFK00_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HET7593691.1"
                     /translation="MSELRYLLQQVYERSKAPPMLAPDPAAMQRLLEQCHRRRYPGKS
                     AILRPGDPANTLFYVIDGSVTVCSEDDQGRELILAYINRGEFIGEMGLFVEQAQREVL
                     IRTRTPCELAEIGYDKLFQLFAGQLRDDCPRILFAIGLQLTHRLLRTSRQVSRMAFLD
                     VTNRIARTLLDLCQEPDAMTHPEGTQIRISRQELSRIVGCSREMAGRVLKQLEDTGGI
                     DVSGKTIVVRGTR"
     gene            complement(6903..7568)
                     /locus_tag="VFK00_11910"
     CDS             complement(6903..7568)
                     /locus_tag="VFK00_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET7593692.1"
                     /translation="MEQAEAARRVVLFDFDGVLIRGDAFSLFMRDCYARARWRKALVL
                     LSSPWLLLVFPFSRRRVMRALVHLGLLGLDEARYRVRVQAFASTLVRRPGNFRRDGLR
                     ALRRHQAAGDRIVIVTGCEDLLVRAVLAELGVADVEVLASRLRAGRFGMRADWHNVGR
                     RKVELLALHGIDAWHVAYGDSDHDLPMLAVAGEAVLVNATPRFCRRVEKALGHAVTRA
                     EWS"
     gene            complement(7571..8374)
                     /gene="trpC"
                     /locus_tag="VFK00_11915"
     CDS             complement(7571..8374)
                     /gene="trpC"
                     /locus_tag="VFK00_11915"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7593693.1"
                     /translation="MNDILRTILDRKREEIAERIAVLPIRALRDRAAAAAPPRGFVRA
                     LQARVAAGQSAVIAEVKKASPSKGVIRPDFDPAAIARSYASGGATCLSVLTDTDFFQG
                     SDDFLQQARTACALPVLRKDFIIEPYQVHEARALGADAVLLIAAALGNAEFANLHALA
                     LELGMDVLLEVHTTADLERAQMLPRPDRTAIGVNNRDLATFETTLETVLRLRHQVPEG
                     RLLVAESGIATREDVARLRAAGIHAFLVGEACMRAPDPGAELARLFTGP"
     gene            complement(8371..>8717)
                     /locus_tag="VFK00_11920"
     CDS             complement(8371..>8717)
                     /locus_tag="VFK00_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593694.1"
                     /translation="RDGKVREYEIEPEQFDIPMASSRNLRVGDAAESKAMLLRVLEGE
                     AGVPRNIVLLNAGAALYAADAAASIAGGIERARGAIDDGSARAKLDEFVAATRTLAGC
                     ESRLPSPEPRTS"
BASE COUNT         1397 a         2893 c         3007 g         1420 t
ORIGIN      
        1 ggtgcgacgc ggcgagctgg acgccaggca gcggatcgaa gcggccgccg gggtcctgca
       61 cagcctggaa aagctcccgg aattcatgac cgaccggcgc gtggccggct actgggcggt
      121 gcgcggcgag ttgccgctca atctggcggt ggcatccctg gcgcggcgcg agcagcgcta
      181 cttccttccg gtgctcggcg aatcgcggca gctccgcttc ggggaatacc gcgacggcac
      241 caggctggac agcaatcgct tcggcatccc cgagcctgat gtaccgaccg aagacctgca
      301 tgggccggcg gacatggacg tgatcctgct gccgctgctc gccttcgacc ggcttggccg
      361 gcgcctcgga accggcggcg gctggtacga caccagcctc gcccacctgc gcgaggtacc
      421 gcgaccggcg acgccgctgc tggtcggggt cggctacgcg ttccaggagg tcgaggccgt
      481 gccgcccgaa ccgtgggacg tcgatgtcga ctacatcgcc accgatgggg aactgatagc
      541 ctgtcgcgct gacggtgcca cctgacgagt cgacgatgcg acgctggctg atgaaaaccg
      601 agcccgacac cttctcgatc gacgacctga agcagcgccg cgtcgaaccc tggaacggcg
      661 tgcgcaatta ccaggcgcgc aacttcatcc gggaaatgac gcgcggcgac gaagtgatga
      721 tctaccactc gagttgcgcg gtgccgggcg tcgcggggct cgccaaggtc gcaagcgcgc
      781 cctatcccga tcccggccag ttcgatccga aggacgatta cttcgatgcc gggagcgatc
      841 gcggcaatcc gcgctggtca ctggtggacg tccggttcgt gcgcaagttc aagcgggtgg
      901 tcacgctgga cgagatcaag caactggaag gcctgggcga cttcgccctc acgcggcgcg
      961 gcaaccgtct gtcgatactg ccggtgagcg atgcacagtg gcgggccatc ctcgaactcg
     1021 aatgaaaggg ggcgcgttgg acatcgaagc gcagaaacgc gcggccggcg aggccgcgct
     1081 cgaatacgtc gaagacaacc aggtgatcgg cgtcggcacc ggctcgacgg tgcggtactt
     1141 catcgagggc ctcgcgcgga tgaagcaccg gctcgacggg gcggtatcga gctccgaaca
     1201 gagcagcgcg ctgttgcgcg agcacggcat ccaggtgctc gatcccaacg cggtcggcac
     1261 gatcggggtg tacgtggacg gagccgacga gtgcgatccg cagcgccgcc tcatcaaggg
     1321 cggcggcggg gcgttgacgc gcgagaagat cctcgctgcg gtcgcgaagc aattcgtgtg
     1381 catcgtggac gcgggcaagt gcgtcgaagt actcggcaag tttgccgtcc ccgtggaagt
     1441 gatccccatg gcgcgcagcc acgtcgcccg ggagatccag cagcgcggtg gcatgccggt
     1501 gtggcggcag ggctgcgtca ccgacaacgg caactggatc ctcgacgtgc acggactttc
     1561 gatcaccgag cctgcgcgag tcgaggccga atggaaccag atcgcgggcg tggtcaccgt
     1621 gggactgttc gcgcagcggc cggcggacgt ggtgctggtg ggcgacggcc gacggctctg
     1681 aggggcgcag aaacgatgaa gcccgccgag gcgggcttca tcgtttctgg agtggcagcc
     1741 ccggatggat tcgaaccacc gaatgcctga gtcagagtca ggtgccttac cacttggcga
     1801 cggggctgtg cagacggatt ttagtgcgaa tcaggagagg ccgcgagcga agcggctgtt
     1861 gccaaaagtc aggccatgga tggctgttcc gatctccatg accgacagtc ggcctgaccg
     1921 cttgcgttcc cgacaacgcc gcgatcaggg atcgatcgca cacttgcggg atcgcgcaaa
     1981 ttaccgcttc gagaactgcg tcgcgcgacg tgccttgtgc aagccgacct tcttgcgctc
     2041 gaccgcgcgg gcgtcgcggg tcatgaaccc cgcacggcgc agcggcgcct tcagggtttc
     2101 atcgtattcg accagcgcac gggcgatgcc gaggcggatc gcgccggcct ggccggtgat
     2161 gccgccgccg gcgacgttga cctggatgtc gaacttgtcg gcggtctggg tgagttccag
     2221 cggctggcgc acgatcatcc gcgacgtttc ccgcccgaag aattcgtcca gcggacggcc
     2281 gttgaccatg atctggccgc tacccttgtt caggaacacg cgggcggcgg aggtcttgcg
     2341 gcggccggtg ccgtagttct gttgcgtcgt cgccatcgtc agatctccag cgccttgggc
     2401 tgttgtgcgg tgtgcgggtg ctcgggtccg cgatagacct tgagcttgcg gaacatcgtc
     2461 cggcccaggg gattcttcgg cagcatgccc ttgacgccga tctcgatcgc gcgttccggg
     2521 tgcgttgcca gcagatcctt gagattggtg gtcttcaggt tgccgacgta gccggtgaaa
     2581 cggtggtagt tcttgtcttc cagcttgttg ccggtcaccg cgaccttttc cgcgttgacc
     2641 acgacgatgt aatcgccggt gtcgacgtgc ggggtgaact ccggcttgtg cttgccgcgc
     2701 agacgacgcg cgacttccga gcacagccgg cccagggtct tgccactggc gtcgacgacg
     2761 agccagtcgc gcttgaccga ttcggccttg gcgctgaagg tcttcatggg aacgatccgt
     2821 acagttgtag ggccgcacat agtgcgggcc gcgccacggg gcgcgacggc agttgacagc
     2881 aacgaaagac cgccaaggat accggcgatg gcggcgccac gcaagatacg gcggtgggcg
     2941 ggactcggga cccgggatcc ggaattccgg ggacccggga ccggggacca gcagcttgtt
     3001 cctgagcggc tgccgtggaa agtcaggcca gggatggccg ttctggtgtc acccaatcgc
     3061 gctgccaaca cgcaacgggc tgcctgaggc gcagcgttca tggacgaacg caaagcgaat
     3121 ccttgctagc atcggccgat gtccgccccc gatgcccgcc agcacattat cggacttgcc
     3181 aatgcctgcg tgaagtgcgg actgtgcctg ccgtactgcc caacctatgc gctggatcgg
     3241 gtcgaaacgg agtccccgcg tggcaggatc gccttgtcgc gccatgccgt ggacggcagc
     3301 gtcgcgccgg acgcggatct gcgcgcccat ctcgaccatt gcctgggctg cctcgcctgc
     3361 gaacgggttt gcccggtgcc cgtccactat ggcgaactgc tgaccgctta ccgcgcggcc
     3421 ctgccgcctg cgtcgccccg accccggcgc atgctgcgcc tgctgactcg gccgcgctgg
     3481 ctccgcacgg gcgccgcact ggcgcggtgg gtcggtgcgc gacattggct gacgccgctg
     3541 gcgcgccggc tcttgccgcg tcattccgcg ctgcgcgcgg cggtttcgct gttgcccgcc
     3601 ctgcccgccg ctccgcgcgc cggaaccgcg gttccggccc cggccgctgc tcgcggcaag
     3661 gtcgcgctgt tcggcggctg cgtgcagggc gcactcgagc gcgaagccca ggatgccgca
     3721 gcgtgcctgc tgtcggcggc cggcttcgag gtccggcgca tggagggact ttgctgcggg
     3781 gcgctggcga cgcataccgg tgattcggtc gatgccgccg ccaaggcacg gcgggtacgt
     3841 gatgcctccg cggccgagag tgtggattgc gtactgactg ccactcctgg ctgcctcggc
     3901 acgctgcgcg aggatctgcc ggggacgcag gtggaggacg cgatggcgtt tctcgaccga
     3961 cagggcgagg cgctggcgtt ccggccactc gaacgcaagg ccgtgctgca cctgccctgc
     4021 acccaggtca atgtcgcgcg cgccgacggt gcggtacgac gcatgctggc gcggattccc
     4081 gggctcgaac tcgacgtatt gccgacgcgc ggcatgtgct gcggagccgc ggggagttac
     4141 atgctggaat tcccggagcg cgcggtggcg cagcgccagc gcgtgttcga aaacctgccg
     4201 gatccgcttc ccggcctgct gctgaccgcc aacatcggct gtcgcctgca cctcggcgcc
     4261 ggcctgggcg aacgcggcac gcccatggag gttcagcatc cgctggtcct gctcgcgcaa
     4321 caattgaagg catgaaccgt ccgacgatcg atacccgtcg cctgggcccc ggcgaccgcg
     4381 tcctggccgc gttccagcgt gcgctcgata ccgtcgccgg cgcggccgtt gccgcgcgcc
     4441 catcgcccgg cgaagagcac ccgttgccgc gcctcgatcc ggcggcacgc cgccacgccg
     4501 ccgggctgat gcgcgtcaac cacaccggtg aggtctgcgc gcaggcgctc tacgacggac
     4561 aagcggcatt ggcgcgcgac tccgtcaccc gtgcccacct cgacgccgcg gccggcgagg
     4621 aaaccgacca cctcgcctgg tgcgcgcaac ggctctcgga actcgacgac cacccgagcc
     4681 tattgaatcc ggtctggtac gccggcagct tcgcgatcgg cgcgctggcc gcgatggccg
     4741 gcgaccgctg gagcctcggc ttcgtcgtcg aaacggagcg gcaggtggag gcccacctcg
     4801 gcgagcacct cgcgcgcctg ccgcccgccg atgcgcgttc gcgcgcgatc ctccgaacca
     4861 tgcaggccga cgaagcccgc cacgccgaca acgcgctcgc gcgcggcgga gaacgggttc
     4921 cgccgccgat ccctgccctc atgcggggcg cctcggcggt gatgaaggcg gtggcctatc
     4981 gggtttgaac tgcgggatct gcgggaacag ggaacgggga gcggtcaagc gggaacggta
     5041 gcggcgaaga tcgcgccttt ctgttccccg tttccctgtc ccctgcctcc cgccgctggc
     5101 ccctacatca agttgcggcc gtggaacagc tcctcgatct cgcggcggag cagcgactcg
     5161 atcttctggc ggtcccggaa cgacaggtcc tcggcgtgcg cctcgaacag gtaggtgtcg
     5221 aggtcgaagt ccttcacgtg catcttggtg tggaacatgt tttcctggta cacgttgacg
     5281 tcgaacatct cgtacttctg gcggatgtgc ttggcgaggt aatcctggat cgagttgatc
     5341 ttgtggtcga tgtagtgctt cttgccgtgg atgtcgcggg tgaagccgcg cacccggtag
     5401 tccatcgtca cgatgtccga ctcgaagctg tcgatcaggt agttcaaggc cttcagcggc
     5461 gaaatcaccc cgcaggtcgc aacgtcgatg tccgcgcgga acgtggcgat cccgttgtgc
     5521 gggtgcgttt ccggataggt gtgcacggtg atgtgcgact tgtccatgtg cgcgaccact
     5581 gcgtccgaga tcatgtcgcg gccgagcttg tcgatcacgg ggtgttcgga aatcaggatc
     5641 gtcaccgagg cgccctgcgg gtcgtagtcc tggcgggcga tattcaggat gttcgcgccg
     5701 atgatgtcgg cgacgtcggt caggatctgg gtcaggcggt cggcatcgta ggcctcatcg
     5761 atgtagtcga tgtagcgatc acgctggtcg ttggtgaccg cgtagcagat gtcgtagatg
     5821 ttgaagctga gcgccttggt gaggttgttg aacccctgca gcttcaagcg gggcagcggc
     5881 ttgaccacgg cagtgtccct cgaaacgggt ggagacggaa tgccgggcgc aaggctgggt
     5941 gcggatgcgc gcgcggcggt atcgggaaga cgcggagtat gcgggaaagt cggctccggc
     6001 gcacccttgc cacttggccg ccgttaatgt ggcacaatcc cggcccaggg gtgaactttc
     6061 cataaaacgt gaagtagttt caggaaccca tgtccgaact acgctacctg ctgcaacaag
     6121 tctacgaacg ctcgaaggcg ccgccgatgc tggcgcccga tcccgccgcc atgcagcggc
     6181 tgctcgagca atgccaccgc cgccgctatc ccggcaagag cgcaatcctg cggccgggcg
     6241 accccgccaa cacgctgttc tacgtgatcg acggctcggt cacggtctgc agcgaagacg
     6301 accagggccg cgagctgatc cttgcctaca tcaaccgcgg cgaattcatc ggcgagatgg
     6361 ggctgttcgt cgagcaggcc cagcgcgagg tgctgatccg cacccgcacg ccctgcgagc
     6421 tcgccgagat cggctatgac aagctgttcc agctgtttgc gggacaactt cgcgacgact
     6481 gcccgcgcat cctgttcgcg atcgggctgc agttgaccca ccgcctgctg cgaacctcgc
     6541 gccaggtcag ccgcatggcg ttcctggacg tcaccaaccg gatcgcgcgc acgctgctgg
     6601 acctgtgcca ggagccggac gcgatgaccc atccggaagg cacccagatc cggatctcgc
     6661 gccaggagct gtcgcgaatc gtgggttgct cgcgcgaaat ggccgggcgg gtcctgaagc
     6721 agctcgagga caccggcggg atcgacgtgt cgggcaagac gatcgtggtg cgcgggacgc
     6781 ggtaattgcc tctcccctgg gtgagggtca ggtaccacgg caggcgtgtt gggtgcgcaa
     6841 cgacccgaac cctcatccgg cgctgcgcgc caccttctcc cgcagggaga agggacagcc
     6901 cctcaactcc attcggcgcg ggtgacggcg tggcccagcg ccttctccac gcgccggcaa
     6961 aacctgggcg tcgcattgac cagcaccgcc tcgccggcca cggcgagcat cggcagatcg
     7021 tgatcggaat cgccgtaggc cacgtgccag gcgtcgatgc cgtgcagtgc cagcagttcc
     7081 actttgcggc ggccgacgtt gtgccaatcc gcgcgcatgc cgaaccggcc cgcgcgcaag
     7141 cgtgacgcca gcacctcgac gtcggccaca ccgagttcgg cgagcacggc gcgcaccagc
     7201 aaatcctcgc aaccggtgac gatcacgatg cgatccccgg cggcctggtg ccggcgcagc
     7261 gcccgcagcc cgtcgcgacg gaaattcccc ggccggcgca ccagcgtaga ggcaaaggcc
     7321 tgcacccgga cccggtagcg cgcctcgtcg aggccaagca gccccagatg caccagtgcc
     7381 cgcatgaccc gccgtcgcga aaacggaaac accagcagca accacgggct cgacagcagc
     7441 accagcgcct tgcgccagcg cgcccgcgcg taacagtcgc gcatgaacag cgagaaggcg
     7501 tcgccgcgga tcagcacgcc gtcgaagtcg aacagcacga cgcgccgcgc cgcctccgct
     7561 tgctccatgc tcacggccct gtgaacagcc gcgccagctc cgcgcccgga tcgggcgcgc
     7621 gcatgcaggc ttcgccgacc aggaacgcgt ggatgccggc ggcgcgcagc cgcgcgacgt
     7681 cctcgcgggt ggcgatgcca ctttcggcca ccagcaggcg accttccggc acctggtgcc
     7741 ggagccggag gaccgtttcg agggtggtct cgaaggtcgc gagatcgcga ttgttcacgc
     7801 cgatcgccgt gcgatccggc cgcggcagca tctgtgcgcg ttcgagatcg gccgtggtgt
     7861 gcacctcgag cagcacgtcc atgccgagtt cgagcgccag cgcatgcagg ttcgcgaact
     7921 cggcgttgcc gagcgcggcc gcgatcagca ggacggcgtc ggcgccgagg gcccgcgcct
     7981 cgtgcacctg gtagggctcg atgatgaagt ccttgcgcaa caccggcagc gcgcaggcgg
     8041 tgcgggcctg ttgcaggaag tcatcgctgc cctggaagaa gtccgtatcc gtcagcaccg
     8101 acaggcaggt ggcgccgccc gacgcgtagc tacgcgcgat cgcggccgga tcgaaatcgg
     8161 ggcggatcac gcccttcgac gggctggctt tcttgacctc tgcgatcacg gcagattgtc
     8221 cggctgcgac acgggcctgc aaggcgcgga cgaacccgcg cggcggggcg gcggccgcgg
     8281 cgcgatcacg caaggcacgg ataggcaaca ccgcaattcg ctcggcgatc tcttcgcgct
     8341 tgcggtcgag gatggtgcgg agaatgtcgt tcacgacgtg cgaggctcgg gactcgggag
     8401 tcgggactcg cagccggcca gcgtacgggt cgccgcgacg aattcgtcga gcttggcgcg
     8461 cgccgaaccg tcatcgatcg cgccgcgcgc acgttcgatg ccgccggcga tcgaagccgc
     8521 ggcgtcggcg gcgtacaagg cggcgccggc gttcaacagc acgatgttgc gggggacgcc
     8581 tgcctcgcct tccagcacgc gcaacagcat cgccttggat tccgcggcat ctccgacccg
     8641 caggttgcgg ctggacgcca tcgggatgtc gaactgctcg ggctcgattt cgtactcgcg
     8701 caccttgcca tcgcgca
//
LOCUS       DASSNH010000317         4391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_430179, whole genome shotgun sequence.
ACCESSION   DASSNH010000317 DASSNH010000000
VERSION     DASSNH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430179"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..2458
                     /locus_tag="VFK00_11925"
     CDS             173..2458
                     /locus_tag="VFK00_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HET7593695.1"
                     /translation="MRRAKTSLAGCVLLVLTGIVPTAFAATDPSFLLAATAKDLEAYF
                     PGELANGHFSTFTSPRGTEGNLSYLVAFMDHGKDDIARPAAIPGWSGIDYRTGASGAW
                     LNLSPVDAATLQDYRQVLDLHDGTLTTSYRYVDHGKATRVNVVEFVSEASPHLAAVRF
                     TVTPEFDGVVELSFPLVLWAPSQPRLALAKLPGDTGLTGDRFRQALHAHGLSLEPAAP
                     ATADRAAVWYHGDTQVLDAAGDTEAMTLRLDGRATNSKRMGEAVAVQLPAGIRPVESK
                     LDRTDYRLALDVRAKVQKGRTYAFTKYVAVSRDGWGGDGKADLALARAARQQGFDAML
                     DAQRATWAKLWQSDIRIDGDPEAQLALHSDLYYLLANAAPEGAWGLGGCGMTTGYVGH
                     VFWDSDTWMFPALVLLHPERAKSIVMFRDRTLPAAQRRARAAGFEGAKYPWEADPQDG
                     SEQILFAAHRLSVGEIHVNADIAIAQWQYWLATHDLAWLREHGWPVIRDVAEFWVSRA
                     TYDKAQARYEIRDVVSLMEPYAHVDDDTFTNAAAAKALRIAAAAATAVGERADPRWTE
                     VADKLDIPFSEAGRHHLDFDPGTPLHDAADNDLAFLMFPSLDLAMGETVRHNDYRIAA
                     APLEQPGATSTSMGLAPLAIAAATVGNSADMTRWLQDNIGPGMLKAPFNVRTETVDNN
                     TGWFLTGSAGFVQSLLYGVTGLRIEENGLVAAYPPVLPTGWKSMTLKNLTFRGRHYDI
                     TIDRDADGKPRLTRRATSVDK"
     gene            2455..4278
                     /locus_tag="VFK00_11930"
     CDS             2455..4278
                     /locus_tag="VFK00_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7593696.1"
                     /translation="MTSAALLADMRRIDAMLRAGELQGAHDRLATIVEEHPDFSEARR
                     LLGGAKLALGDAAGAETVLREAAAVEPAWPPLLTMLGELLLNSGRPAEAEPLLLRAAT
                     AATADPRAAWVLARHYNDRQRPAQALAIVAPFCAAGHFAPELAAQHVSALFALDRGAE
                     AIDFYQDALTRAPTNVAAAAALATALQSANRHAEAERVSRRAIGRGCRSAALHHARAR
                     SLVAMGEFERAEAALRDCLQLDPQRVDAQDELARLAWMRSGDVAEATSMLDQAIARFG
                     SDDALWAAKAAILQGAGDPRAAHACLAPRLERPHAPPALLVRAGLAAVEFDPRAALEL
                     ARRALRALPTDTAARKLEAAAELGVGDAGAALALCEALLAEDPDEQYLIALRTTAWRM
                     LGDERYARLCDYGTWVVPYRLEVPPPWQGLGEFLADLQRSLGKLHDALRHPLLFQSLR
                     RGTETTGDLARSTDPVVQALFKAFDAPIRDYIARIGHGSDPLPRRNLGGYRFNGSWSV
                     RLHASGFHHNHVHPRGWISSACYIALPEGMDNPADRAGTLTFAEPGILTTPPLPAEHE
                     VRPEPGTLVLFPSYFWHGTVPFSGERTRLTVAFDAVPAPAT"
BASE COUNT          705 a         1486 c         1507 g          693 t
ORIGIN      
        1 gacaagggac tggatgcgga atacgcgccg gtgctgccgc cggactggaa atcggtgacg
       61 ctgaagaacg tcagtttccg gggtgagcat tacgactaca ccatccggcg cggcgaggat
      121 ggcaaggcaa ggctggtgcg caaggagctt tgacgtgatg cggtcgggtc ggatgagacg
      181 cgcgaagacg agcctggctg gttgtgtcct gctggtgcta accggcatcg ttccgacggc
      241 gttcgccgcc accgacccga gcttcctcct cgccgccacc gcaaaggatc ttgaggcgta
      301 tttcccgggc gaactcgcca acggccattt ctcgaccttc acttcgccgc gcggcaccga
      361 aggcaacctg tcgtacctcg tcgcgttcat ggaccatggc aaggacgaca tcgcgcgacc
      421 cgcggcgatt cccggctgga gcggcatcga ttaccggacg ggcgcctccg gtgcgtggtt
      481 gaacctgtcg ccggtggatg ctgcgaccct gcaggactac cggcaggtcc tggacctgca
      541 tgacggcacc ttgaccacga gctaccgcta cgtcgaccac ggcaaggcga cgcgcgtgaa
      601 cgtggtggag ttcgtcagcg aggcttcgcc ccacctcgcc gcggtccgct tcacggtcac
      661 ccccgagttc gacggcgtgg tggagttgtc gtttccgctg gtgttgtggg cgccctcgca
      721 gccgcgcctg gcgctggcga aactgcccgg cgataccggg ctgacggggg accgcttccg
      781 gcaggcgttg catgcgcacg ggctgtcgct ggaaccggcg gcgccggcca cggccgatcg
      841 cgctgcggtc tggtatcacg gcgatacgca ggtattggac gccgccggcg acacggaagc
      901 aatgacgctt cggctggacg gacgggcgac caacagcaag cggatgggcg aggccgtggc
      961 ggtgcagttg ccggccggca ttcgtccagt cgaatcgaaa ctggatcgga ccgactaccg
     1021 gctggcgctg gacgtgcggg cgaaggtcca aaagggcagg acctacgcgt tcaccaaata
     1081 cgtcgcggtt tcgcgcgacg gctggggtgg cgatggcaag gcggaccttg cgctggcccg
     1141 ggccgcacgc caacagggct tcgacgcgat gctcgatgca cagcgcgcga cgtgggcgaa
     1201 gctgtggcaa tcggacatcc gcatcgatgg cgatcccgag gcccaactcg cgctgcattc
     1261 ggacttgtat tacctgctgg cgaatgccgc gcccgaaggc gcctggggcc tcggtggctg
     1321 tggcatgacc acggggtacg tcgggcacgt gttctgggac agcgacacct ggatgtttcc
     1381 ggcgctggtg ttgctgcatc cagagcgtgc gaagtcgatc gtgatgttcc gcgaccgcac
     1441 cttgcccgcc gcgcagcgac gcgcccgcgc ggccggtttc gaaggcgcga agtacccgtg
     1501 ggaagccgat ccccaggacg gcagcgaaca aatcctgttc gcagcccacc gcttgagcgt
     1561 cggcgaaatc catgtcaacg ccgacatcgc gatcgcgcag tggcaatact ggcttgcaac
     1621 ccatgacctc gcctggttgc gcgagcacgg ctggccggtg atccgcgacg tcgccgagtt
     1681 ctgggtcagc cgcgcgacgt acgacaaggc gcaagcccgt tatgaaatac gcgatgtcgt
     1741 ttcgctgatg gagccttacg cccatgtgga tgacgacacc ttcaccaatg ccgctgcggc
     1801 aaaggcgttg cggatcgccg cggccgcggc gacggcggtc ggagaaaggg ccgatccgcg
     1861 ctggaccgaa gtcgcggaca agctcgatat accgttttcg gaagcgggcc ggcatcatct
     1921 cgatttcgac cctggcaccc cgctgcacga tgccgccgac aacgacctgg cattcctgat
     1981 gttcccgtcg ctggacctcg ccatgggcga aacggtgcgg cacaacgact accggatcgc
     2041 tgccgcgccg ctggagcaac cgggcgcgac atcgacgtcc atgggtctcg cgcccctcgc
     2101 gatcgccgcc gccacggtgg gcaattcggc ggacatgacg cgctggctgc aggacaatat
     2161 cggtccggga atgctgaagg ccccattcaa cgtgcgcacg gaaacggtgg acaacaacac
     2221 cggctggttc ctcaccggtt cggcaggctt cgtgcaaagc ctgctctacg gcgttaccgg
     2281 cttgcggatc gaggagaacg ggttggtcgc ggcctatccg cccgtgctgc caacgggatg
     2341 gaaatcgatg acgttgaaga acctgacctt ccgcggcagg cactatgaca tcaccatcga
     2401 ccgcgacgcc gatggcaagc cgcggctgac gcgccgggcg accagcgtgg acaaatgact
     2461 tccgccgcgc tcctcgcgga catgcggcgc atcgacgcca tgctgcgcgc gggggaattg
     2521 cagggcgcgc acgatcggct cgcgacgatc gtcgaagagc acccggattt ttccgaggcg
     2581 cgacgattgc tgggaggcgc caagctggcg ctcggcgatg ccgccggcgc cgaaacggtt
     2641 ctgcgcgaag cggccgcggt cgaaccggcg tggccgccgc tgcttacgat gctgggcgaa
     2701 ctgttgctca acagcggccg gcccgcggaa gcagagccat tgctcctgcg cgccgcgacc
     2761 gcggcgacgg ccgatccccg cgcggcgtgg gtgctcgccc gccattacaa cgaccggcaa
     2821 cggccggcgc aggcgttggc gatcgtggcg ccgttctgcg ccgctggcca tttcgcgccg
     2881 gagttggcgg cgcagcacgt gtcggcgctg tttgccctcg atcgtggcgc cgaagcgatc
     2941 gatttctacc aggatgcctt gaccagggcg ccgacaaacg tcgcggcggc cgccgcgctc
     3001 gccaccgcgc tgcaatcggc gaaccgccac gccgaggcgg agcgcgtcag ccgccgtgcg
     3061 atcggtcgcg gctgccgcag cgccgcgttg caccacgccc gcgcccgcag cctcgtggcc
     3121 atgggcgaat tcgagcgcgc cgaggcggcg ctgcgcgatt gcctgcaact ggatccgcag
     3181 cgcgtcgacg cgcaggacga gctcgcgcga ttggcctgga tgcgctctgg cgacgtcgcc
     3241 gaggcaacct cgatgctcga ccaggcgatc gccaggttcg gcagcgacga tgcattgtgg
     3301 gcggccaagg ccgccatcct gcaaggcgcg ggcgatccgc gcgcggcgca tgcgtgcctt
     3361 gccccgcggt tggaacgacc gcatgcgcca ccggcgctgc tggtgcgcgc cggtcttgcg
     3421 gcagtggaat tcgacccccg cgcggcgctg gaactggccc gacgcgcact gcgcgcgctg
     3481 cccaccgaca cggcggcacg caaactcgag gcggcggccg aactgggcgt cggcgacgcc
     3541 ggcgccgcgc tcgcgctttg cgaagcgttg cttgccgaag accctgacga gcagtatctg
     3601 atcgcgcttc ggaccaccgc gtggcggatg ctgggcgacg aacgttatgc acgcctgtgc
     3661 gactacggga catgggtggt tccgtaccgg ctggaagtcc ctccgccctg gcaaggcctc
     3721 ggcgaattcc ttgccgatct gcaacggagc cttggcaagc tgcacgacgc gcttcgccat
     3781 ccgctgttgt tccagtccct gcgtcgcggc acggagacca ccggggacct ggcgcgcagt
     3841 accgacccgg tggtgcaggc gctgttcaag gctttcgacg cgccgatccg cgactacatc
     3901 gcccgcatcg gccatggttc cgatccattg ccgcgccgaa acctgggcgg ctatcgcttc
     3961 aacggaagct ggtcggtgcg gctgcacgcc agcgggttcc accacaacca cgtgcacccg
     4021 cgcggatgga tttcatccgc gtgctacatc gccctgcccg aaggcatgga caatccggcc
     4081 gacagggcgg ggaccctgac cttcgccgag ccgggcatcc tcaccacccc gcccctgccc
     4141 gcggaacacg aagtgcgtcc ggagccgggc acgctggtgc tgtttccgtc ctatttctgg
     4201 catggcacgg tgcctttttc cggcgagcgg acgcggctga ccgtggcgtt cgacgcggtg
     4261 cccgcgcctg cgacgtagct cgacaggcgc agtgccggaa tccgggtgcg cgacacggag
     4321 gccgcgattc ccgcatcgct ccatccgggc atcgcccgcc gcgcccgatg atcggcggac
     4381 ggtgcccgcc c
//
LOCUS       DASSNH010000318        29113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1293095, whole genome shotgun sequence.
ACCESSION   DASSNH010000318 DASSNH010000000
VERSION     DASSNH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 29113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29113
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293095"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFK00_11935"
     CDS             <1..390
                     /locus_tag="VFK00_11935"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET7593697.1"
                     /translation="HPRKVHVPFWTLVGGILGGGIMYFVEWYSAAIDYPINVGGRPPF
                     SWPAFLPPTFEMTLLWASVFGVFAMLIGNGLPRLHHPLFAVPEFERGSSDRFFLLLRA
                     HDPLFDLPRTRDFLATLAPLSIIEVPE"
     gene            654..1301
                     /locus_tag="VFK00_11940"
     CDS             654..1301
                     /locus_tag="VFK00_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7593698.1"
                     /translation="MSLACVLTLTLAGCERGMHEMYEQPKYKPLTPSPLFADGNSSRP
                     QVDGTVPHSAGALAGTTSGREGAVQPLPPSGPAVGVDAQGKPLAQAGRAAAPGYSNPL
                     PITPALLARGRQRFNIYCAPCHSRAGDGDGMIARRGFPHPPSYHSDRLRNAPDSHFYQ
                     VITHGYGIMYPYADRITPHDRWAIVAYIRALQLSQHAPVADLGPADLAKLKGAVK"
     gene            1298..2449
                     /locus_tag="VFK00_11945"
     CDS             1298..2449
                     /locus_tag="VFK00_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593699.1"
                     /translation="MTRAWLPRRFEWLLPPVGVLALAGCLLFGLWGDMHQALLSYLFA
                     FVFFTGLSVGSLAVAMIQPLTGGAWGWYIRPQLLAALRTLPLMAVLVVPILFGMHDLY
                     VWTHADALAHDELLRKQTWWLTSTFFVIRTVGYFVLWLLVWFAFARALRRNARMSVIA
                     APGAIVYALTTLMAAFDWISSLTPHWHSDTFGMLVATGWMLGAAALAVLCATCSPYEE
                     DRHPPPLLHDLGTLLCMFVLGWSYLAFMQYLTIWVADLPAENAWYIPRTLTTWRWFAW
                     FLIAFGFAIPFAVLLSRRAKRSRAAMGTVAGMLLAANLVYAFWLVVPGFRPGGFSLRW
                     TDVLAVLGIGAPWFCIYLCGLRVTRVPALRPDADEPAPVLRQRVERTDG"
     gene            2442..2921
                     /locus_tag="VFK00_11950"
     CDS             2442..2921
                     /locus_tag="VFK00_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593700.1"
                     /translation="MADTREAPFGHEPPGVATRRVVGVVLVLGTAVAIALVALWLALT
                     YWVMPNHAQLKTRPAVLPPAPRLQPHPDNDLAALREEKRALLHGYLWTDDAHRFARIP
                     IGRAMEIYVGEQKAGTGNREQGLGGGTPAVSASTSGTGDRRPGAGMPAASASSGGHR"
     gene            3083..3907
                     /locus_tag="VFK00_11955"
     CDS             3083..3907
                     /locus_tag="VFK00_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7593701.1"
                     /translation="MLALLLAWLPAAGFASTQAHVRAPPDLLKRIGFDQDLGARAPMD
                     GKFRDAHGQPASLRGLAQGNPILLVPGYFHCANLCGIVRAGVANAVKASGFVPGRQFN
                     VVLVSIDPRDTPKSALATKRNDAFAHPGAHVQQWHYLTGPEATSAALMRSIGFRYFYD
                     SRNGQFDHAAGVVLLTPDGKVSQYLFGVKFPARTLRLGLVHASDHRIGNLVDQFLLLC
                     CRYDASTGRYTLVIHRVMQGLGIATALLLAGFLLVLRRMERRRGGLDLPDDGEPRS"
     gene            3904..4872
                     /gene="coxB"
                     /locus_tag="VFK00_11960"
     CDS             3904..4872
                     /gene="coxB"
                     /locus_tag="VFK00_11960"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7593702.1"
                     /translation="MSALLPDASSVAPRVDVLFWYMIGLCGFVAVGIFATMIYFGIKY
                     RQGSRADRTGKHKQSMGIEVAWTLVPFGMFLAVFIWSLFVFARLQSPPAAAQTVYVVA
                     KQWMWKIQHPGGQREIDALHVPLGQPIRLVMISQDVIHSFSVPAFRVKQDVLPGMYTN
                     LWFTPTETGRFSLYCNQYCGLDHSRMIGDVVVMQPAAYARWLEKNPAPESVAARGAAL
                     FRSRGCSGCHGANASVHAPDLDGLYGRTVHLADGGTVIADDRYIHDSILLPDKQIVAG
                     FAPIMPSFEGQLSEQEVLALVAYIKSTSATAQQPPSVGSESTHERH"
     gene            4859..6487
                     /locus_tag="VFK00_11965"
     CDS             4859..6487
                     /locus_tag="VFK00_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7593703.1"
                     /translation="MSAIDAGTVPQPEPESLAPSFYSGKRTLRGWLFTRDHKRIAILY
                     TLSVTLFFFLGGAAAAMIRYDLIVPSGELPTEVYNKAFTFHGVVMVWFFLLPSIPVTL
                     GNFLLPIMIGAKDLAFPRINLFSWYLFIGGGAFVIAALWAGGVDTGWTFYTPYSTMFS
                     NSNVYLAIIGVFVTGFSTIFTGLNFIVTTHTMRMPGMTWMRLPLFVWGLYAVSVVMVL
                     ATPVLAMTLALVGLERVVGVGVFDPSLGGDPLLFQHLFWFYSHPAVYMMILPAMGLAS
                     EIISCFTSRRVFGYKFMTWSMLAIAVIGFMVWGHHMFVSGMSVYAAMVFSLLSFMVAV
                     PSAIKIFNWTATLRKGDIRFTAPMLYALGFVGLFLLGGLTGLILAAPALDEFLHDTYF
                     VIAHFHYVMVGGSVMAYLGGIHFWWPKVTGRLYSERLAKFAAVLMFVGFNVTFFPQYL
                     LGYRGMPRRYHSYLPQFQELNVISSGGAVLLAISYTLPVLYLLWSLRYGKKAPPNPWD
                     ARGLEWQTSSPPPKENFLHPPVPREAYAYDEPAP"
     gene            6484..7164
                     /locus_tag="VFK00_11970"
     CDS             6484..7164
                     /locus_tag="VFK00_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7593704.1"
                     /translation="MNATLADSPLEEARRAHLEEQFDEPDQQHSAASLGMWIFLATEM
                     LFFGVLFTSYVVLRVLHPAGFAEASRETDLLLGSGETAALLTSSITIVIALRAIKLGQ
                     RGVATLFLSLTAALGIGFLVIHGFEYHAEWKEHLVPGVRFLKFGRHAHAVELFEVLSY
                     LLTGWHSIHLVIAVSVVLVLAARVWHGAFGPDHFTTLELAALYWHLVDIIWMFIFPLL
                     YLIGRAPG"
     gene            7161..7625
                     /locus_tag="VFK00_11975"
     CDS             7161..7625
                     /locus_tag="VFK00_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593705.1"
                     /translation="MNPQRAPKSRTRFLGRLVVALGALQALLAIRRYRRAVHASPTAA
                     APEDSRMRSSRTRPEVKSLKTFAFAWLGLLVLLAFTVGSAFVDLGAANIAINLLVSVV
                     MMLLLMTLFMHESKARRLTQLASAAGLLWVVLMIGLSLADYLTRVHIPAPWS"
     gene            complement(7871..8143)
                     /locus_tag="VFK00_11980"
     CDS             complement(7871..8143)
                     /locus_tag="VFK00_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593706.1"
                     /translation="MTEPTKFLSSRFLSASTLLGASVRNLQDESLSFGGLLGMGDKLF
                     AIPWENFRVDAETERLVLDISKDRLKGAPGFDADHWPDFAERQPGP"
     gene            8395..10341
                     /locus_tag="VFK00_11985"
     CDS             8395..10341
                     /locus_tag="VFK00_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7593707.1"
                     /translation="MAATSARVSRISICLLAGLWAGAASGQGTLQELDKLSLQDLGNI
                     VVTSVAKNPEPLNGAAAAVYVITHDDIMRSGATTLPEILRLAPNLGVFRLSPSNYIVT
                     SRGLSGSQNAQNFPNKLLVLIDGRSVYNPLFSGIYWDQQYVLPENIDRIEVISGPAGA
                     LWGANAVNGVINIITRNAADTQGGLLEFGLGSHEAAASVQYGGKLGDFAWYRVYAHDF
                     RQRAFEDSNGHDARDGWTAPQAGFRIDWEAGGGNVATLEGNASESREGQPGGPNVLAN
                     AANLLGRWRHQSANGSTFSLQAYVDHGHSGASLTSGGGTITTWDIEAQQDFALGEHHH
                     ITWGVGDRIYRYKLLPSIRSDASLLWSQPADTQNLANAFVQDQIALGASMQATVGIKA
                     EDDPYSGLSWMPSMRLSWKSARGMLLWASVARSVRTPTVFDTSVLEQSNTPDGSVDFI
                     RGNPDYRKEKLTAYEAGLRNQWGARAALSVSVYYNDYDDLRSIEATPVNFLPLYWGNG
                     MQGHTYGIDAWSAYSIADWWKLSAGLSLEREHFRFKPGATGLLGTAIVGNDPEHRAFL
                     RSSMNLGQHWKFDADLRRVGALPDPHVPGYTELDARLAWAPDDDWELSLRGMNLLHPW
                     HQEYVLGQADRIGRTVFLDARLTF"
     gene            10457..11002
                     /locus_tag="VFK00_11990"
     CDS             10457..11002
                     /locus_tag="VFK00_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET7593708.1"
                     /translation="MAECVLLATLLLAGAEAFAQSRAADVSLEYAVKATYLYKLAPFV
                     NWPQDTFTSPDAPFGICLVGPDPFHDFLEKAVAGRKLGSHPFVVRELGAGTPADACQI
                     VFIRDSRPEAVRLALRRFEGAPVLTVTDSNAPDDAGSIVQFVMDNGRVRFDVDTTAAI
                     RNHLTISSKLLSLALAVKGAG"
     gene            11004..12512
                     /locus_tag="VFK00_11995"
     CDS             11004..12512
                     /locus_tag="VFK00_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7593709.1"
                     /translation="MDSLSRPQRVPLKPITTIAAAGLLLLIGVIAMLYQFQSVHTERR
                     RQLTSQARTLAASVTAAIAFNDHAAAQEYVKALMLDPRVDAVAVYNEAGRLVAGAHMP
                     GSAPIPTPRTAPGKRPQLAKGLLLVDVPARQGPTTVGSVLLRGTGTPWAMQLAKISGV
                     ALLTLMGALMLSVVALAQRALGHANANLHRRAEQLATANASLKAEIEQRSRAEEALRQ
                     SQKMEAVGQLSGGIAHDFNNVLMVVKTALVLLEKRLAQSYTPLERFEESARERVAAGP
                     RQDAARPLQVLAEGLELAGQARSRRQQIEHYLETAHGGIEKAAALTGRLLAFARSQPL
                     SPKPMQLDVLVRGMQTLLEHSVGANIEIRYQLHSDWHVLCDANQMENAILNLVINARD
                     AMPDGGEITISTADLRPEGGQGSDGQATGQVRLRVTDTGTGMSEEVRSRALDPFFTTK
                     PVGKGTGLGLSTILGYVMQSNGQLEIDSKAGIGTTIDIVLPREVSSVSMEVT"
     gene            12512..12898
                     /locus_tag="VFK00_12000"
     CDS             12512..12898
                     /locus_tag="VFK00_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7593710.1"
                     /translation="MDSTVQSAVRILLVEDEALLREYAAESLREEGFDVQAVGDGEAG
                     LKALQSGAPIDVLLSDIRLPGVSGYELAEAGRMLRPDLKMILMTGYAPGLPQTLEHTV
                     HCVLQKPFRIDTLTGVITEALAAPAA"
     gene            13498..13860
                     /locus_tag="VFK00_12005"
     CDS             13498..13860
                     /locus_tag="VFK00_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593711.1"
                     /translation="MAANLQSMVNSERSAVRHALAAERVRAGRCRAALECAKGSGLRA
                     RCRRKLDATLGRAGKLALALVDMGEAVPMAQVFTDFDGRLLDAMDTVMRKHPRHAARA
                     YARERPTVTHRRSRIQTS"
     gene            13903..14313
                     /locus_tag="VFK00_12010"
     CDS             13903..14313
                     /locus_tag="VFK00_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7593712.1"
                     /translation="MATKAGEVMTQECFVVAPEQTIAEAAVLMRDKDVGSVPVGSNDK
                     LVGMLTDRDIVVRATAQNMGPETPVREVMSDKIRYCFDDEDVEEIAQNMADLQVRRLP
                     VVNREKRLVGFISLSNITGSGQGPTVLRGVAQPH"
     gene            15287..17650
                     /locus_tag="VFK00_12015"
     CDS             15287..17650
                     /locus_tag="VFK00_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7593713.1"
                     /translation="MIMANDIREDDDTMVFPAEGSQGSAGVFAAAGHPPPGGASQRSD
                     DESPSAARDREGDARQVHAPNERLFAVIQELQARNEELARANSELNSLIAGTGIGTLF
                     LDNELRVRRFTPHLASTFELVAANVGRPFAELAPRFDTENLLADAEYALRDAVAVERE
                     SGGKDGHFYRVRVQPGHVGNGKADGVALTFVDISERERRDEEVRSLNLELEAGKAFAE
                     SIVATVREPMLVLDQDLRVVMASRAFYRDFLTTPEATVHELLYELGDRQWDIPELRRL
                     LDDVLPERTDVDALEVRHDFGTGDRIMLLNARRLRQQPGVAPLILLTFEDITDRSRAM
                     QALAASESKYRTLFDSIDAGFCIIEMIYDRAGRPSDLRHLEVNPAFARNNGLTDVVGK
                     TLRGDLGIELEQSWFDAYSRVAETGESVRFQQYSNAFNRWFDVNVFRVDLPELRRVAV
                     LFSDITERKRADRATVRLASIVDTSDDAIISKDLDGVVNSWNHGAEAMFGYTASEAIG
                     HTVASLIIPADRQDEEDDILRRIRRGEAVDHFETVRRHKDGVLLDVSVTISPLRDRRG
                     RIIGASKIARDIGERKQYERHRELLIQELNHRVKNTLASVQALAAQTFRGSGMRESVA
                     TFEARLLAMSRAHDVLTRQSWQGAAIREVVGDAVAAWAGKSGERASFEGPELRLRPAV
                     ALALAMALHELATNAVKYGALSNKKGKVSIEWSVSQDPQACFRLRWREQGGPLVHAPA
                     RRGFGSRLIEYALARDLGGTVDLTFRKEGVECRIEAPLEEIREEGTV"
     gene            17650..18024
                     /locus_tag="VFK00_12020"
     CDS             17650..18024
                     /locus_tag="VFK00_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7593714.1"
                     /translation="MEDGDSLDGCRVLIVEDDAMISMMLQDLLEDMGCSVVGVASRLA
                     EAQRKGEAGGFDVALLDVNLRGEHTFPVAESMQERRQPFVLTTGYATTILPDPLRRAT
                     LLQKPYRRQDLERALWDAMHGD"
     gene            18864..20252
                     /locus_tag="VFK00_12025"
     CDS             18864..20252
                     /locus_tag="VFK00_12025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7593715.1"
                     /translation="MKSATATGSVLLVDDNESFVRMLVEAGRQAGCKVYHAANVEAAR
                     GLLAEGKIFDLLLVDIMLPDGNGLELVESMDLADQGRIAIVTGMPSVESAVRVLNTPV
                     VDYLVKPIPAATLQELLLGAHDRAVARSRVSRPLGDMVGGSIQMRTLFDQIRQVGPLD
                     VSVLIHGESGTGKELVARALHEQSGRAGRFVPVNCGAITHDLLGSQVFGHERGAFTGA
                     VQSHTGFFEQADGGTLFLDEITEMPAQLQVYLLRVLESRMLTRLGGTREFPVDVRLVA
                     ATNRDPRAAVAAGVLRQDLYYRLIEFPLYIHPLRERREDIALLARHFLDRLNRKYGTS
                     KTFQPDALVRMTEAAWPGNVRELRHAVQRHFILSGDSPQVGFRHDDAPVTAESDADSV
                     HFRVGMTFEEVEREMLLKTLSRCGNNKSRTANVLGITAKTIYNRLLRYRAEGLIDDSL
                     LHGGPAEHDSAG"
     gene            20693..21394
                     /locus_tag="VFK00_12030"
     CDS             20693..21394
                     /locus_tag="VFK00_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7593716.1"
                     /translation="MPVSILPDSEAEVADRDAAILPERDFNATFGEMTHAVLNALNAV
                     AAAAELSRLLLRRDEPAEASKSLARVEPECLRAARLLREGRTFLTFRLAAPGEVDIAA
                     LLNECVERFAGRAEIVGGAGLPALRGDPEALRKLFTELLENAFAFGATHVRVTPTEIG
                     SDAVRIDFLDDGPGIAMPAARAFTPFFSTQPAQHSGLGLPLARRIAEAHGGRIGIGES
                     EGGALLWIELPLPEA"
     gene            complement(21512..22570)
                     /locus_tag="VFK00_12035"
     CDS             complement(21512..22570)
                     /locus_tag="VFK00_12035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7593717.1"
                     /translation="MTCKPFVLFALALSVAATVHAQASAHQARIDALMSRYAGDVPGA
                     SLLVVKDGKPLVERGYGYANLEKHIKAGPETNYRLASVTKQFTAASILVLKQDGKLEL
                     DDPIRKYLPELPTSDDGITIENLLAHSSGLVDYEDLIPSSRTTQIDDNDVLRMIASQD
                     RLYFQPGSAHRYSNGGYVLLGLIVERLSGMDLADFMRRRIFEPLGMDHTLLYEHRRGP
                     KPANRAYGYSLIADKWTRTDQSVTSATRGDGGVYSNVSDMAKWDAALYTDKLLDAESR
                     KLAFTPKDPVDDPDVLYGFGWRLSGGTTWHSGETMGFRNVIIRWPEQHLSVVILSNRN
                     QFQPYPLALAIGELFLAR"
     gene            complement(22587..23258)
                     /locus_tag="VFK00_12040"
     CDS             complement(22587..23258)
                     /locus_tag="VFK00_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7593718.1"
                     /translation="MATVARADAPPRISPAKTMADAGMVDIRSLVPDIAENIHYAGSD
                     NFTGAPVPGYRAAKCFLLKPAAEALARVEHALREQHARLKIWDCYRPARAVAAFVRWA
                     HDGDADSKAAHYPNVDKAKLLGEYIAPVSGHSRGATMDLTMMQCDARDRNCKPLDMGT
                     PFDLFDPLAHTDSPQATASQHANRQRLLHAMAAQGFENYSAEWWHYTYTMHPQPTTLY
                     DLPVQ"
     gene            complement(23296..24852)
                     /locus_tag="VFK00_12045"
     CDS             complement(23296..24852)
                     /locus_tag="VFK00_12045"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7593719.1"
                     /translation="MNFRTNKWFFGMVALAGYAAIAHAATPPANHNADIDRLVDAVVA
                     YYHLPGLAVGVIDHGKVVYTREVGKLASGKPIDDETLFEIASNTKAMTSTLLARLVQQ
                     GKLRWDDPVTKYLPSFRMHDPWVTKHMQVGDLLVHHSGLSEGAGDLMLWPTPNHFTPA
                     DVVAGLRYIKPAYSFRAGYAYDNTLYIVAGQVAAAAGGAPYATLMRSEIFQPLGMTRC
                     QIGTWNRDEVGNVADPHTIENGHFVASRHRGAIVHETTMDSAGGVRCGLHDMLTWAMN
                     WLAPTPQQLQWLTPKQRHAEWTPYTPMPISKLRHEWDGTLFWGYGYGWRIGDVDGEMT
                     VSHTGTLSGMYSAVTLLPFKQSGYVFMINAEAGNARSVFNEALAKHFTAPGRARSVES
                     YADELKRADEQRRISRIPDTSSRQPATPADLKNELGVWRDPWFGEVHICAARTAVEFA
                     SAKSPMLTGEVMRVGDKYLVHWNHGDAEAWLRFPAQAGGTLRMAKVDPDADFSYDYED
                     LGFKREGSCD"
     gene            complement(25019..26302)
                     /gene="lysA"
                     /locus_tag="VFK00_12050"
     CDS             complement(25019..26302)
                     /gene="lysA"
                     /locus_tag="VFK00_12050"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7593720.1"
                     /translation="MTATTAHADLACPEAACLDGVELPALARRVGTPCQVYSASVIRG
                     RVDALQRALRGLDARVCYAAKANSNIAILQLMATAGFGADIVSGGELWRCLRAGIPAE
                     HIVFSGVGKTVEEIADALEAGVARFNVESADELQLLQRLAAVRGITAHASARINPNVD
                     ARTHAKIATGKSENKFGVGVEEAREWFANSAALTHVRLDGLHVHIGSQMLGLDPIRAA
                     LRRVAELWRELGTAGHAIRSIDVGGGLGVCYRADQDHPVAIDDYADAIRGALADFDGR
                     ILLEPGRWLVAEAGVLLTRVIRVKRGQRRTFLVLDAAMTELIRPSLYDAWHDIVPLVD
                     GSRPLLAYDIVGPVCETGDTFARDRTLPECRAGDLVMIKATGAYGASMASTYNSRPLA
                     AEVLLDRGSYAVVRRRQTLEEMIAGEQAAQQWEHA"
     gene            complement(26374..28242)
                     /gene="ggt"
                     /locus_tag="VFK00_12055"
     CDS             complement(26374..28242)
                     /gene="ggt"
                     /locus_tag="VFK00_12055"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7593721.1"
                     /translation="MPGKTLPRSRVKHSTASYRRRWQYRRLSLLAAGLFAFGIAGCST
                     NDTTHASRVASASTAPAAATATTSPFVHLPADTRAALENVKPVVAQHAMVVSDQHLAT
                     KAGVKILKEGGNAIDAAVAVGYALAVTQPCCGNIGGGGFMTIHLANGENLFLNFREKA
                     PLKATPTMFLDAQGKVVEGRSTDTYLGVGVPGTVMGLDTALEKYGTMSLKQVIQPAIE
                     LARDGFVLEEGDAEIINNDLEALNKYPESAAVFGNGGKPWKAGDRVVQKDLANTLEKI
                     AKGGTDAFYKGSIARAVVKASDAHGGILGMKDFASYNVEWMKPLQCEYRGYTILSAPP
                     PSSGGTTMCLALNILKPYPVADWGYASVPFVHYFAEAERRAFATRNTYLGDPDFVDIP
                     MDKLMSAEYAASLRATIEPDKATPSSEIKSFLPPSEGNNTTHYSVMDGHGNAVAVTYT
                     INFLFGIKQIAGDTGFYLNDEMDDFTAKPGASNGAGLVQGEADQVEPGKRPLSSMTPT
                     VVLKAGKPFMLSGSPGSATIISTTMQSIINVVDFGMNMQQAVNAPHVHMQWYPDVVYV
                     GPGMLTPESKRQLEAMGYTLKERHLGVLESILVNPETGMLEGASDPHRSAGLAAGY"
     gene            complement(28412..>29113)
                     /locus_tag="VFK00_12060"
     CDS             complement(28412..>29113)
                     /locus_tag="VFK00_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7593722.1"
                     /translation="LWDLESKLTGVSVWARLARPSHVPLVTALTVSLDAPQAMEAAAA
                     RIADAKLIKIKVDAGDPAARIEAVRRAAPSARLIVDPNESWTSDVLRDVQSVLERASV
                     ALLEQPLPAGADDALLEYSGRIPVCADEACHVTTDLPKLRNRYQAVNIKLDKTGGLTE
                     AWRLLRAARAQGFRIMVGCMVCSSLGIAPALEIAREAEFVDLDGPLWLENDYSGGVEL
                     QDGLLVPPALGFWGS"
BASE COUNT         4735 a         9490 c         9779 g         5109 t
ORIGIN      
        1 cacccgcgca aggtgcacgt tccgttctgg acgctggtcg gcggcatcct cggcggcggg
       61 atcatgtact tcgtcgagtg gtactcggcg gcgatcgatt accccatcaa cgtgggcggc
      121 cgcccgccct tcagttggcc cgcgttcctt ccgccgacct tcgagatgac gctgctgtgg
      181 gcgtcggtgt tcggcgtgtt cgcgatgctg atcggcaacg gcctgccgcg cctgcaccat
      241 ccgttgttcg cggttcccga gttcgaacgc ggcagcagcg atcgcttttt cctgctgctg
      301 cgcgcccacg atcctctttt cgatctgccg cgcacgcgag atttcctagc gaccctcgcg
      361 ccgctctcga tcatcgaggt gccggaatga ggatccacat cttcaatgat ggaagccatg
      421 agatggcggc cgcggagttg aaccctctcc cccatacggt ttcatcgttt gggggagagg
      481 gcagggtgaa ggggaattgc ttcgcgccac cgcgtgtgcg tgcggagcgg gagcctggcc
      541 ccctcacccc agccctctcc cccgagcaga gaacgctcgg gggagaggga gagacgctgc
      601 gcggcgcaac aggggggaag gcgtcggtag ctcgcgtagg agcccgcatc gcgatgtcgc
      661 tcgcctgcgt gctgaccctc acgctcgccg gctgcgagcg cggcatgcac gagatgtatg
      721 agcagccgaa gtacaagccg ttgacgccga gccccttgtt cgcggacggc aattcctcgc
      781 gtccgcaggt ggacggcacg gtgccgcatt cggcgggtgc gctggccggg accaccagcg
      841 gacgcgaggg cgccgtccag ccgctgccgc cttccgggcc agcggtcggc gtggacgcac
      901 aaggaaaacc gctggcccag gccgggcgcg cagccgcacc cggttactcg aacccgttgc
      961 cgatcacgcc ggcgctgctc gcgcgcggtc gccaacgttt caacatctac tgcgcgccct
     1021 gccacagccg cgcgggcgac ggcgacggca tgatcgcgcg gcgcggattt ccgcacccgc
     1081 cgagttacca cagcgatcgc ctgcgcaacg cgccggacag ccacttctac caggtgatca
     1141 cccatggcta cgggatcatg tatccgtacg cggaccggat cacgccgcac gaccgctggg
     1201 cgatcgtcgc ctacatccgc gcattgcagt tgagccagca cgccccggtt gcggatctcg
     1261 gtcccgccga cctcgcgaaa ctcaaggggg cagtgaaatg acacgcgcct ggctgccgcg
     1321 ccgcttcgaa tggctgctgc cgcctgtcgg cgtgctggcg ctcgccggtt gcctgctgtt
     1381 cgggctttgg ggcgacatgc accaggccct gctgtcgtac ctgttcgcct tcgtgttctt
     1441 cacgggcctc agtgtcggca gcctcgcggt ggcgatgatc cagccgctga ccggcggagc
     1501 ctggggttgg tacatccggc cgcagttgct ggcggcgctg cgcacgctgc cgctgatggc
     1561 ggtgctggtc gtcccgatcc tgttcgggat gcacgacctg tatgtctgga cccatgccga
     1621 cgcgctcgcg catgacgagc tgctgcgcaa gcagacctgg tggctgacct ccacgttctt
     1681 cgtgatccgc acggtcggct atttcgtgtt gtggctgctg gtgtggttcg cgttcgcgcg
     1741 cgccctgcgc cgcaacgcgc ggatgtcggt gatcgccgca cccggcgcga tcgtgtacgc
     1801 gctcaccacc ctgatggccg cgttcgactg gatctcgtcg ctgacgccgc actggcattc
     1861 ggacaccttc gggatgctgg tcgccaccgg ctggatgctg ggtgccgccg cgctggcggt
     1921 gctgtgcgcc acatgctccc catacgagga agaccggcat ccgccgcccc tgctgcacga
     1981 cctcggcacc ctgctgtgca tgttcgtcct tggctggtcg tacctcgcct tcatgcagta
     2041 cctcacgatc tgggtcgccg atctcccggc cgagaacgcg tggtatatcc cgcgcacgct
     2101 gaccacctgg cgctggttcg cctggttcct gatcgcgttc ggattcgcga tcccgttcgc
     2161 ggtgctgctg tcgcgccgcg ccaagcgcag ccgcgcggcg atgggtacgg tcgccgggat
     2221 gctgctcgcc gcgaacctcg tctatgcgtt ctggctggtg gtgccgggtt ttcgcccggg
     2281 aggtttttcg ctgcgatgga ccgatgtcct ggccgtgctg gggatcggcg cgccgtggtt
     2341 ttgcatctat ctttgcgggc ttcgggtgac gcgggtcccc gcgctgcgtc cggacgcgga
     2401 cgaaccggcg ccggtgctac ggcagcgggt ggagcggacc gatggctgac acgcgcgaag
     2461 cgcccttcgg ccacgagcca ccgggtgtcg ccacacgccg ggtggtcggt gtcgtcctcg
     2521 tcctgggcac ggccgtggcc atcgcgctgg tcgctttgtg gctggcgctg acgtactggg
     2581 tcatgcccaa ccatgcgcag ttgaaaaccc ggcccgcggt gttgccaccg gcgccgcgac
     2641 tgcagccgca tcccgacaac gacctcgcgg cactcaggga agagaagagg gcgctgctgc
     2701 atggctacct gtggaccgac gacgcgcatc gattcgcgcg gattccgatc ggacgggcga
     2761 tggagattta tgtgggggag cagaaggcgg gaacggggaa cagggagcag ggactcgggg
     2821 gcggcacgcc tgccgtttct gcttcgacat cggggaccgg ggaccggaga cctggggccg
     2881 gaatgccggc tgcatccgct tcgtcgggag ggcaccggtg aaaatgtcga ctgccagcgc
     2941 aaacccgacc ggcctctgcc gcggtacggc atgcgccgca catgcagatg tttcgaaaag
     3001 gccccaatcg gggaaaaagc tagcgcttcg acgtgcacga acgtcctgca agaatttttg
     3061 catccggcac gccgcgccga ccgtgcttgc gttactgctg gcctggctcc cggcagccgg
     3121 attcgcgagc acgcaagcgc atgtgcgggc gccacctgat ttgttgaagc ggatcgggtt
     3181 cgaccaagat ctcggcgcgc gggcgccgat ggacggaaaa ttccgtgacg cccacggcca
     3241 gcctgccagc ttgcgcggtc tcgcgcaggg caacccgatc ctgctggtcc cgggttactt
     3301 ccattgcgcc aacttgtgcg ggatcgtgcg ggccggtgtg gccaatgcgg tcaaggcgag
     3361 tggcttcgtg ccggggcggc agttcaacgt ggtactcgtc agcatcgatc cgcgcgatac
     3421 accgaagtcc gcgctcgcga ccaagcgcaa cgatgcgttc gcgcatcccg gtgcccacgt
     3481 ccagcagtgg cattacctca ccgggccgga agcgacgagt gccgcgctga tgcgcagcat
     3541 cggcttccgg tatttctacg acagccgcaa cggccagttc gaccacgcgg ccggcgtggt
     3601 gctgctgacg cccgacggca aggtgtcgca gtacctgttc ggcgtgaagt tcccggcccg
     3661 cacgctgcgg ctcgggctgg tgcacgcctc cgaccaccgc atcggcaacc tcgtcgacca
     3721 gttcctgttg ctgtgttgcc ggtacgacgc ctcgaccggc cgctacacgc tggtgatcca
     3781 ccgcgtgatg cagggcttgg gaatcgcgac cgccttgctg ctggccggct tcctgctggt
     3841 gttgcggcgg atggagcggc gccgcggcgg cttggacctt cccgacgacg gggagccgcg
     3901 atcatgagcg cactgctgcc ggatgcctcg agcgtcgcgc cgcgggtcga cgtgttgttc
     3961 tggtacatga tcggcctgtg tggcttcgtc gccgtcggaa tcttcgcgac gatgatctat
     4021 ttcgggatca agtatcgcca gggttccagg gccgaccgca ccggcaagca caagcagtcg
     4081 atgggcatcg aagttgcctg gacgctggtt ccgttcggga tgttcctcgc ggtgttcatc
     4141 tggagcctgt tcgtgttcgc gcggctgcag tcgccgccgg ccgccgcgca aaccgtgtac
     4201 gtggtcgcca agcagtggat gtggaagatc cagcaccctg gcggccagcg cgaaatcgac
     4261 gcactgcacg tgccgctcgg ccagccgatc cggctggtga tgatctcgca ggacgtgatc
     4321 cacagcttct cggtgccggc cttccgggtg aagcaggacg tgctgcccgg catgtacacg
     4381 aacctctggt tcacgccgac cgagacgggg cgcttttcgt tgtactgcaa tcaatattgc
     4441 ggcctcgacc attccaggat gatcggcgac gtggtcgtga tgcagcctgc tgcctacgcg
     4501 cgctggctgg agaagaaccc ggcgccggag agcgtcgccg cgcgcggcgc tgcactgttc
     4561 cgcagccgcg gctgcagcgg ctgccatggc gcgaacgcct cggtgcatgc gccggacctg
     4621 gatggcctgt acgggcgcac ggtgcacctc gccgacggcg gcaccgtgat cgccgacgac
     4681 cgctacatcc acgactcgat cctgctgccc gacaagcaga tcgtcgccgg tttcgcgccg
     4741 atcatgcctt cgttcgaggg ccagttgagc gagcaagagg tactcgccct ggtggcctac
     4801 atcaagtcca cctcggccac cgcgcagcaa ccaccatccg tcggctcgga gagtacccat
     4861 gagcgccatt gacgccggca ccgtcccgca acccgaaccg gaatcgctcg cgcccagctt
     4921 ctacagcggc aagcggacct tgcgcggatg gctgttcacc cgcgaccaca agcggatcgc
     4981 gatcctctac accctgagcg tcacgctgtt cttcttcctg ggcggcgcgg cggccgcgat
     5041 gatccgctac gacctgatcg tgccgtcggg cgagcttccg accgaggtct acaacaaggc
     5101 cttcacgttc catggcgtgg tgatggtgtg gttcttcctg ctgccgtcga tcccggtgac
     5161 gctggggaat ttcctgctgc cgatcatgat cggggccaag gacttggcgt tcccgcggat
     5221 caacctgttc agctggtacc tcttcatcgg tggcggcgcg ttcgtgatcg ccgcgctgtg
     5281 ggctggcggc gtcgacaccg gctggacctt ctacacgccg tactcgacga tgttttccaa
     5341 cagcaacgtg tacctcgcga taatcggcgt gttcgtcacc ggcttctcga cgatcttcac
     5401 gggcctcaat ttcatagtca ccacccacac gatgcggatg ccgggcatga cctggatgcg
     5461 gctgccgctg ttcgtgtggg gcctgtacgc cgtcagcgtg gtgatggtgc ttgcgacccc
     5521 cgtgctggcc atgacgctcg cgctggtcgg gctggagcgg gtggtcggcg tcggcgtttt
     5581 cgatccgtcc ctgggtggcg acccgctgct gttccagcac ctgttctggt tctactcgca
     5641 ccccgcggtg tacatgatga tcctgccggc gatgggtctc gccagcgaga tcatttcgtg
     5701 tttcaccagc cgccgcgtgt tcggctacaa gttcatgacc tggtcgatgc tcgcgatcgc
     5761 ggtgatcggc ttcatggtgt ggggccacca catgttcgtc tccggcatgt cggtgtacgc
     5821 ggcgatggtg ttctcgctcc tgagtttcat ggtcgcggtg ccgtcggcga tcaagatctt
     5881 caactggacg gcgaccttgc ggaagggcga tatccggttc acggcgccga tgctgtatgc
     5941 gctgggtttc gtcgggctgt tcctcctcgg tggcctgacc ggattgatcc tcgccgcacc
     6001 ggcgctcgat gagttcctgc acgacaccta cttcgtgatc gcgcacttcc actacgtgat
     6061 ggtgggcggc tccgtgatgg cctacctcgg cggcatccac ttctggtggc cgaaagtgac
     6121 cggccggctg tattcggagc ggctcgcgaa attcgcggcg gtcctgatgt tcgtcgggtt
     6181 caacgtgacc ttcttcccgc agtacctgct cggctaccgc ggcatgccgc gccgctacca
     6241 ctcgtacctg ccgcagttcc aggagttgaa cgtcatctcg agcggtggcg cggtgctgct
     6301 cgcgatctcg tacacgctgc cggtcctgta cctgttgtgg tcgctcaggt acggcaagaa
     6361 agcccccccc aatccatggg atgcgcgcgg gctcgagtgg cagaccagct cgccgccgcc
     6421 caaggaaaac ttcctgcacc cgccggtgcc gcgcgaagcc tacgcttacg acgagcccgc
     6481 gccatgaacg ccacgcttgc cgattcaccg ttggaagagg cgcgccgtgc gcacctcgag
     6541 gagcagttcg acgagccgga ccagcagcac agcgcggcat cgctcgggat gtggatcttt
     6601 cttgccacgg agatgctgtt cttcggcgtg ctgttcacca gctacgtggt cctgcgggtg
     6661 ctgcatccgg ccggtttcgc cgaggccagc cgcgaaacgg atctcctgct cggctccggc
     6721 gaaaccgcgg cgctcctgac cagcagcatc acgatcgtga tcgccctgcg cgcgatcaag
     6781 ctgggccagc gcggcgtggc cacgctgttc ctgtcgctga cggcggcgct cggcatcggc
     6841 ttcctggtga tccacggctt cgaataccac gccgaatgga aggagcacct ggtgcccggc
     6901 gtccgcttcc tcaagttcgg acggcacgcg catgccgtgg agctgttcga agtgttgtcg
     6961 tacctgctga ccggctggca ctcgatccac ttggtcatcg cggtgtcggt cgtgctggtg
     7021 ctggcggcaa gagtgtggca cggcgccttc gggccggatc atttcacgac tctcgagctc
     7081 gcggcgctgt actggcacct ggtcgacatc atctggatgt tcatctttcc attgctgtac
     7141 ctcattggga gggcgcccgg atgaatccgc agcgtgcccc gaaatcgagg acgagatttc
     7201 tcggaaggct ggtcgtcgcg ctgggcgcgc tgcaggcgtt gctggccatc cggcgctatc
     7261 gccgtgccgt ccatgcttcc cccaccgcag cagctccgga ggattcgcgc atgcgttcgt
     7321 cccggacgag accggaagtg aaatcgctga aaacctttgc cttcgcgtgg ctcggattgc
     7381 tggtgctgct ggccttcacc gtcggcagcg cgttcgtcga cctgggcgcc gccaacatcg
     7441 cgatcaacct gctggtgtcg gtagtcatga tgctgctgct gatgaccctg ttcatgcacg
     7501 aaagcaaggc gcgcaggctg acccagctcg cctcggcggc cgggctgctg tgggtcgtat
     7561 tgatgatcgg tttgtccctt gccgattacc tgacccgcgt gcacatcccg gcgccgtggt
     7621 cgtgatgcag cggggaatcg ggactcggac gttgcaggag ctggccacgc cgcgaccggc
     7681 tccctcgggc gcggaatgtc gcggatgcaa gcacagcttt cgccgccatg gccgttcctc
     7741 cgttttccat ccggcggcgt gccgttccgt aggagcgggg cacgcccgcg atgcggcggg
     7801 ccggacagta ccccgtcaat ccggatggac gatgcgcgct ccgtcgtgcg ggggccgggt
     7861 cccatagtgt tcacggacct ggttggcgtt cggcgaaatc cggccagtgg tcggcgtcga
     7921 atcccggcgc gcccttcagc cggtccttgg agatgtcgag gaccagccgt tccgtctcgg
     7981 catcgacacg gaagttctcc catgggatgg cgaacagttt gtcgcccatg ccgagcaggc
     8041 cgccgaagga cagggactcg tcctgcaggt tcctgacact ggcgcccagc aaggtggatg
     8101 cggagaggaa acgggaagaa aggaatttcg tgggttcggt catgtcgcgg tactccttgc
     8161 ggtggtgcgc tcgggcctgc ttccgggcag gccccgctac agccccggct tcccgcaccg
     8221 aatgcgtccg atatccggcc cagcgcgaga ccagcgaggc aaggcgatct ccgggcatgg
     8281 ggacagccgc gcggggctct ccgtataatg cccgggcccg gcgcactcga ggtgttcgtc
     8341 gatccacgaa aggggaagca catgcggtca ggaagatccc cggggggtga ccgggtggcg
     8401 gcaacgtccg cgcgggtatc caggatttcc atatgcctgc tcgcgggact atgggccggt
     8461 gccgcatccg ggcagggcac cctccaggaa ctggacaagc tctcgctgca ggacctcggc
     8521 aacatcgtgg tcacttcggt ggcgaagaat ccggagccgc tgaacggggc ggccgcggcg
     8581 gtctacgtca tcacccacga cgacatcatg cgttccggcg ccacaacgct gccggagatc
     8641 ctgcggctcg cgcccaacct cggcgtgttc cggctaagcc ccagcaacta catcgtcacc
     8701 tcgcggggcc tcagcggcag ccagaatgcg cagaatttcc cgaacaaact gctggtgctg
     8761 atcgacgggc gcagcgtgta caacccgctg ttctccggga tctactggga ccagcagtac
     8821 gtgctgccgg agaacatcga ccggatcgag gtgatcagcg gtccggccgg cgccttgtgg
     8881 ggcgccaacg cggtcaacgg cgtgatcaac atcatcaccc gcaacgcggc cgacacccag
     8941 ggcgggctgc tggagttcgg cctgggcagc cacgaggcgg ccgcctcggt gcaatacggc
     9001 ggcaagctcg gcgatttcgc gtggtatcgg gtgtacgcgc acgacttccg ccagcgcgcg
     9061 ttcgaggaca gcaacggcca cgatgcgcgg gatggctgga ccgcgccgca ggcaggcttc
     9121 cgcatcgact gggaggcagg tggcggcaac gtggcgacgc ttgaaggcaa cgccagcgaa
     9181 agccgcgagg gccaacccgg cgggccaaac gtcctcgcaa acgcagccaa cctgttgggg
     9241 cgctggcggc atcaatcggc gaacggctcc acgttctcgt tgcaggcgta tgtcgatcac
     9301 gggcattccg gcgcatcgct caccagcggc ggtggaacca tcacaacctg ggacatcgaa
     9361 gcgcaacagg attttgcgct gggcgagcac caccacatca cctggggcgt cggcgaccgc
     9421 atctaccgct acaaactcct cccgagcatc cgcagtgacg cgtcgctgtt gtggagccag
     9481 ccagccgaca cgcagaacct cgccaatgcg ttcgtgcagg atcagatcgc gttgggcgca
     9541 agcatgcagg cgaccgtcgg gatcaaggcg gaggacgatc cgtactcggg cctttcatgg
     9601 atgccgagca tgcgtctctc atggaaatcc gctcgcggca tgctcttgtg ggcgtcggtg
     9661 gcgcggtcgg tgcgcacccc gaccgtgttc gacacgagcg tgctcgagca gtccaacacg
     9721 ccggatggtt cggtcgattt catccgcggc aatccggatt accgcaagga gaagctcacc
     9781 gcctacgaag ccggcctgcg caaccaatgg ggtgcccgcg ccgccctgtc cgtgtcggtg
     9841 tactacaacg actacgacga cctgaggtcg atcgaggcca cgccggtcaa tttcctgccg
     9901 ctgtattggg gcaacggcat gcaggggcac acctacggca tcgatgcgtg gtccgcgtat
     9961 tccatcgccg actggtggaa gctgagtgcc gggctttcac tggagcgtga gcatttccgg
    10021 ttcaagccgg gcgcgaccgg cttgctgggg accgccatcg tcggcaacga tccggaacac
    10081 cgcgcattcc tgcgctcgtc gatgaacctc gggcagcact ggaaattcga tgccgacctc
    10141 cggcgggtcg gcgctctgcc ggacccgcac gtgcccggct acaccgaact cgatgcgcgc
    10201 ctcgcgtggg cgccggacga tgattgggaa ttgtcgctga gaggaatgaa tttgctgcat
    10261 ccctggcacc aggagtacgt gctcgggcaa gccgaccgca taggacgcac tgttttcctc
    10321 gatgcccggc tgacattctg aaggcgcgcg catgatccat acgacgccga tgcccgctct
    10381 gcgcgtggcc gccgcgcctg cagcacccgc cgcggcggcg gccccgcggc gtccgccccc
    10441 gcgctggcgg ctgcgtatgg cagaatgcgt acttttggcg acgctgctgc tggcgggtgc
    10501 agaggcattc gcgcagtcgc gggccgccga cgtctcgctc gaatacgcag tgaaggcgac
    10561 ctatctctac aaactggcac cgttcgtgaa ctggccgcag gacacgttca cctccccgga
    10621 tgcaccgttc ggaatctgcc tggtcgggcc cgaccccttc cacgatttcc tggaaaaggc
    10681 ggtcgcgggc cgcaagctgg gttcgcatcc gttcgtcgtc cgcgaacttg gcgcgggtac
    10741 gccggccgac gcttgccaga tcgtgttcat ccgcgacagc cggccggagg cggttcggct
    10801 ggcgctgcgg cgcttcgagg gcgcgccggt actgacggtc accgattcga atgccccgga
    10861 cgacgccggc agcatcgtgc agttcgtgat ggacaacgga cgcgtgcggt tcgatgtcga
    10921 tacgacggcg gcgatccgca accatctcac gatcagttcc aagctgctca gcctcgcgct
    10981 ggccgtgaag ggcgcaggct gacatggaca gcctgtcgcg cccccagcgg gtccccctca
    11041 aaccgatcac gaccatagcc gccgcgggat tgctgctcct gatcggcgtg atcgcgatgc
    11101 tgtaccagtt ccagtcggtg catacggagc gtcggcgcca gctcacctcg caggccagga
    11161 cactcgccgc gagcgtcacc gccgcgatcg cattcaacga ccatgccgcg gcgcaggagt
    11221 acgtcaaggc gctgatgctc gacccgcgtg tggacgcggt ggccgtgtac aacgaagcag
    11281 gccgcctggt ggcgggcgcc cacatgccgg gcagtgcgcc gattccaacc ccccgcacgg
    11341 cgcctggaaa gcgcccccag ttggccaaag gcctgctgct ggtggacgtt ccggcccgcc
    11401 agggaccgac caccgtcggc agcgtcctgc tgcgtggcac cggcacccct tgggcgatgc
    11461 aactcgccaa gatcagcggc gttgccctgc tcaccttgat gggggccctg atgctgagcg
    11521 tggtcgcgct ggcgcagcgg gcgctcggcc atgccaacgc gaatctccat cgccgtgcag
    11581 agcaactggc caccgccaac gccagtttga aggcggaaat cgaacaacgc tcccgggccg
    11641 aggaagcatt gcggcagagc cagaaaatgg aagcggtggg ccagctctcc ggcggaatcg
    11701 cccacgattt caacaacgta ttgatggtcg tcaagaccgc gctcgtgttg ctggaaaagc
    11761 ggctggcgca aagctacacg ccccttgagc gcttcgagga gtccgcgcgc gagcgggtag
    11821 cagcaggtcc aaggcaggat gctgcgagac ccctgcaggt gctggcggag ggactggaac
    11881 tggccggaca agcgcgatcg cgccggcagc agatcgagca ctacctcgag accgcccacg
    11941 gcggcatcga gaaggctgcg gcgctcaccg gccgcctcct ggccttcgcg cgcagccagc
    12001 ccctgtcgcc caagcccatg caactggacg tgctggtacg ggggatgcaa accctgctcg
    12061 agcattcggt aggggcgaat atcgagatcc ggtaccagtt gcattccgat tggcacgttt
    12121 tgtgcgacgc caatcagatg gagaatgcaa ttctcaacct cgtgatcaac gcccgcgacg
    12181 cgatgcccga tggcggggaa atcaccatca gcacggcgga cctgcgcccg gaaggcgggc
    12241 agggctcgga cggccaggcg accgggcagg tgcggttgcg ggtgaccgac accggcaccg
    12301 gcatgagcga ggaggtgcgc agccgggcgc tggatccctt cttcacgacc aagccggtcg
    12361 gcaaaggcac cggcctcgga ttgagcacga tcctcggtta tgtgatgcaa tcgaacggac
    12421 aactggaaat cgacagcaag gcagggatcg gtactaccat tgacatcgtg ctcccgcgcg
    12481 aagtcagcag cgtttccatg gaggtgacgt aatggattcc acggtccaat cggccgtccg
    12541 catcctgttg gtcgaagacg aggcgctgct ccgcgaatat gccgcggaaa gccttcggga
    12601 agaaggcttc gacgttcagg ccgtgggtga cggcgaagcg gggctcaagg ccctgcaatc
    12661 gggtgcgccg atcgacgtgc ttctcagcga catccggctg ccgggggtga gcggctacga
    12721 actcgccgag gcgggccgga tgttgcggcc ggacctcaag atgatcctga tgaccggtta
    12781 cgcgccgggg cttccgcaga ccctggagca cacggttcac tgcgtattgc agaaaccctt
    12841 ccggatcgac acgctcaccg gcgtgatcac cgaagccctg gcggccccgg ccgcctgacc
    12901 gcatttcccc gcgcggcgtc gttcgtcgcc tgccgcgggt gtttcccttc ccctcattcc
    12961 cccgctgtcc gccgcaaggc ggacgccgcg tccggcgggc agtgcccgcg ctatgtgcct
    13021 tccctcgctg ccgcgcatcg ccgtgagacg gaaccgtggt cgcgctgggc gtgaatgcac
    13081 gttccccgac cgccgagccg ggcattccgc atccgcgcct ccgcaaaacc cgtcgaaatt
    13141 tttcgggcgt tgcgggaaac catttccctc cgcaaccggt tgccggccgc gcgatcgcca
    13201 cagtcgcggc tttgcagctt ggcacggttc gtgagaggga gccggtgcag cacgcgcgca
    13261 gggttatcgt cggtgacgag ttggccctcg agacatcgtt cccgcggtgc acggaagcgc
    13321 cgcccgcgga tcgaatgcaa cactcggtcc gcattgccgg atccggatgc gcgccggatg
    13381 cggcatgccc tggacaggtc caggaatgat ctgcccaggg cagctttgcc gaggcccgca
    13441 cgggtgatgg ataccggagg caacggatgc tgcctcccga acatgcaagg agcgatcatg
    13501 gcggcaaatc tgcaaagcat ggtgaactcg gaacgaagtg cagtgcggca cgcgctggcg
    13561 gcggagcggg tgcgcgccgg ccgctgccgc gcggcactcg agtgcgcgaa aggctccggt
    13621 ttgcgcgctc gctgccggag gaagctggat gccaccctcg ggcgcgcggg gaaactggcg
    13681 ctggcgctgg tcgacatggg agaggccgtt cccatggcgc aggtcttcac ggacttcgat
    13741 gggcgcttgc tcgatgcgat ggacacggtc atgcgcaagc acccgcgcca cgcggcgcgg
    13801 gcatacgcac gcgagcggcc gacagtcacg caccgacggt cccgcatcca gacctcatga
    13861 accacaacca tggcaatccg caacagcgga aggagcagga cgatggcaac caaggcaggt
    13921 gaagtgatga cgcaggaatg ctttgtcgtc gctccggaac agacgatcgc ggaggcggcg
    13981 gtcctgatgc gcgacaagga cgtcggaagc gtcccggtgg gttcgaacga caagctggtc
    14041 gggatgctta ccgaccgcga catcgtggtc cgcgccaccg cgcagaacat ggggccagag
    14101 acgccggtcc gggaagtcat gagcgacaag atcaggtatt gcttcgacga cgaggacgtg
    14161 gaggaaatcg cgcagaacat ggcggacctg caggtccggc ggctgccggt ggtcaaccgc
    14221 gagaagcggc tggtgggctt catctcgctc agcaacatca ccggatcggg ccagggtccg
    14281 accgtgttgc gtggcgtcgc gcagccgcat tgacggatgc ttcgatgggg acgcccgcct
    14341 ggcgaaggcc ggacaccgtc cgccgccgtc aaaccaatcg gcgggcatga ccggcccttc
    14401 acgccccggc cgtagggcat gtccaccgcc atccggcggg gcgtcccgcg atgttcgtca
    14461 cccgggggat ccccatggca ctgtccgcct tcagccttgc ttgcacgctg gtgcggtggc
    14521 tggagccgtc cccggtccgg aagccgctgg ctggggccgc gcatgccgat gcagcagcaa
    14581 ggcgtggact actccgctgc cagaggaatc tttttcttcc ctagtccgga atgttttacc
    14641 ggatcgaggc gcttgggagg cgctggagct cgattttggc ggcccggcgg gttggcacgg
    14701 ttcttgagta aagaggcact gcaatgcacc gggcggtttg cccagccctt ccccccggca
    14761 tggtctctcg atggcccggc gcattgcgac accggaccgt gccccgcggc accgccggtg
    14821 tccccggcag acgcaagact gccgtacgag ccacaaggat gcgtggccat caaggacgac
    14881 gcggcaggga gccgcaccga aaccgtcgtg acgacggagt ccggggcagg gaagctccac
    14941 catgctgccg caaggaccgc ggcgcacaag gaagacgcgg caggatgccg cacgggcccc
    15001 gccatcccgg cggggcccgg gacagggaag tccaaagacg tagccccacg gaacggggcg
    15061 aacaaggact cggcaggaag ccgtgcggag ccgtcgcgtt ggcgcgggtt ccgggcaagg
    15121 atgctcggcg gggccggccg tgcaagcggc cggccccgcg gatttttcgt acccgtcgca
    15181 tgccgtcgag ttcacgcacc ggcggcacgc gtcgggcgtg cagtgcggat ggggcccggg
    15241 tgttcggcga gggccgcgct tgacgcgcca tggatggaat gcggcgatga tcatggcgaa
    15301 cgacatccgt gaggacgacg acacgatggt attcccggca gaagggtccc aaggctcggc
    15361 gggagtcttc gcggcggcgg gtcatcctcc gccgggcggc gcatcgcagc gatcggatga
    15421 tgaatcgccg tctgccgcaa gggatcggga aggcgacgcg cgccaggtgc atgcacccaa
    15481 cgagcgactg ttcgccgtca ttcaggaact gcaggcccgc aacgaagaac tcgcgcgcgc
    15541 caacagcgaa ctcaacagcc tgatcgccgg cacggggatc ggcacgctgt tcctggacaa
    15601 cgagctccgg gtccggcgct tcacgccgca tctggcctcg actttcgaac tggtggcagc
    15661 caacgtgggg cggccgttcg cggagctggc gcccaggttc gacaccgaga acctcctcgc
    15721 cgacgccgag tacgcgctgc gcgacgcggt cgcggtggaa agggaatcgg gcggcaagga
    15781 tggccatttc tatcgggtgc gcgtacaacc gggccatgtc ggcaacggca aggccgacgg
    15841 cgttgcgttg accttcgtgg acatcagcga gcgcgagcgc cgcgacgagg aggtccggtc
    15901 gctcaacctg gaactggagg cgggcaaggc gtttgccgag agcatcgtcg ccaccgtgcg
    15961 ggagccgatg ctggtgctcg accaggatct ccgcgtggtg atggccagcc gcgccttcta
    16021 ccgcgacttc ctcactacgc ccgaagcgac tgtccacgag ctgctgtacg agctcggcga
    16081 ccgccaatgg gacatccccg aactccggcg cctgctggac gacgtcctgc ccgaacgcac
    16141 cgacgtcgat gccctggagg tacgccacga cttcggcacc ggcgaccgga tcatgcttct
    16201 caatgcgcgc cggttgcggc aacagccggg cgtggcgcct ttgatcctgc tgacattcga
    16261 ggacatcacc gaccgctcgc gggcgatgca ggcgctcgcc gcgtcggaaa gcaagtatcg
    16321 caccctgttc gactccatcg acgcgggttt ctgcatcatc gagatgatct acgaccgggc
    16381 cggtcggccc tccgacctgc gccacctgga agtcaacccg gcgttcgccc gcaacaacgg
    16441 cctcaccgat gtcgtcggga agaccttgcg cggcgacctc ggcatcgaac tcgagcagtc
    16501 gtggttcgac gcctattcga gagtcgccga gaccggcgaa tcggtccggt tccagcagta
    16561 cagcaatgcc ttcaatcgct ggttcgacgt gaacgttttc cgcgtcgacc tgccggagct
    16621 gcggagggtg gcagtgctct tctcggacat caccgaacgc aagcgcgccg atcgtgcgac
    16681 ggtgcgcctg gccagcatcg tcgatacctc cgacgacgcg atcatcagca aagacctcga
    16741 cggtgtcgtc aacagttgga accatggcgc cgaggcgatg ttcggctaca cagcctcgga
    16801 agcgatcgga cataccgtcg cctcactgat cattcccgcg gaccgccagg acgaggaaga
    16861 cgacatcctg cggcggatcc ggcgcggaga ggccgtcgat catttcgaaa ccgtgcgccg
    16921 gcacaaggat ggcgtcctgc tggacgtctc ggtgacgatt tcgccgctgc gcgacaggcg
    16981 cggtcggatc atcggcgcct ccaagattgc gcgtgacatc ggtgagcgca agcagtacga
    17041 acgccaccgc gagctgctga tccaggagct caaccaccgg gtcaagaaca ccctcgcgag
    17101 cgtgcaggcg ctggcggcgc agaccttccg cggctccggg atgcgggagt ccgtggccac
    17161 cttcgaggca cggctgctcg cgatgtcgcg cgcgcatgac gtgctgaccc gccagagctg
    17221 gcagggcgcg gcgatccgtg aagtggtggg cgacgcggtg gcggcgtggg cgggcaagtc
    17281 cggcgaacgc gcgtcgttcg agggacccga gctgcgcctg cgcccggccg tggcgctggc
    17341 tttggccatg gccttgcacg agttggccac caatgcggtc aagtacggcg cgctgtcgaa
    17401 caagaagggc aaagtgtcga tcgaatggtc ggtttcgcaa gacccgcagg cctgcttccg
    17461 cctgcgctgg cgggagcagg gcggcccgct cgtgcacgcg ccggcgcggc gcggcttcgg
    17521 gtcgcgcctg atcgaatacg cgctggcgcg cgacctcggc ggcacggtgg acctgacgtt
    17581 caggaaagag ggtgtcgagt gcaggatcga ggcgccgctc gaggaaatcc gcgaagaagg
    17641 aacggtgtga tggaagatgg cgatagcctc gacggctgcc gcgtgctgat cgtcgaagac
    17701 gacgcgatga tttcgatgat gttgcaggac ctgcttgaag acatgggctg cagcgtggtc
    17761 ggcgtcgcgt cgcgcctggc ggaagcacag cgcaagggtg aagccggtgg cttcgacgtg
    17821 gcgctgctgg acgtcaacct gcgcggcgag cataccttcc cggtcgccga gtcgatgcag
    17881 gaacggcgcc agccattcgt gctgaccacc ggctacgcga cgacgatcct gccggacccg
    17941 ctgcggcgtg ccacgctgct gcagaaaccc taccggcgcc aggacctcga gcgcgcgctc
    18001 tgggacgcga tgcacggcga ctaacgccgc tctcgcaacg ttttgcgatg cggtcgggtt
    18061 tctcccgggg cgagtccggg gtcaacccaa cctacgcacg cttgtgtagg ttggtgccga
    18121 gcgcggcgaa gcccaacgct cgaccgatcc cgcacgtagg caacgggcgc accgaagcgc
    18181 gacgatgttc gagccgttgg gcttctcccg gaacaaggcc gggatcaacc caacctgcgc
    18241 acgcttttgc aggttggcgc tgagcgcagc aaagcccaac gatgtcgaga tgttgggcag
    18301 cagatctcaa cggaaaaaga ttcctcgacg tccccgaatt taccgcgcgc gaacgcgcaa
    18361 tgccgccgta cgcggttccc ggccggcacg attcctgcca gtggtcacca tgtccgcaat
    18421 gaagcgggtg cgtgccggca tccaatcgtt cggaacgggc gccggccctc tcccatgaca
    18481 aggagttccc ggatggagaa ccgtcaatga aacgaaactg cgaagatcgc ggatgcggct
    18541 gcgacgtcgc agcgggccat ccctgccgca gcgggcaaca gcgccgccag gcgccggcag
    18601 aatcccggtc atccggcgaa acgccgcacg atgcctgcag ccgcgggcgt ccgggctgca
    18661 gcggctgacg ggctcacgcg gccacagccg tcacttccgc catgcgtccc ttgcaccgcc
    18721 ggagctttga aagctccgct gaacggaacc ccaacctgcg cagcgatttg gtcgccggga
    18781 aaacatttct tggcgaatgc ttaaccttgc cgggtttccg gcctattatt ctcgttccct
    18841 tgccgcgcat taccgggaac gcgatgaaat ccgccaccgc gacgggcagt gtcctgcttg
    18901 tcgacgacaa cgagagcttc gtgcgcatgc tggtggaagc gggtaggcag gccggctgca
    18961 aggtgtacca cgccgccaac gtggaagccg cccgcggcct gctcgcggaa ggcaagattt
    19021 tcgacctgct gctggtcgac atcatgttgc cggatggcaa cgggctggaa ctggtcgaga
    19081 gcatggacct cgccgaccag ggccggatcg caatcgtcac cgggatgccg agcgtcgaga
    19141 gcgcggtgcg ggtgctgaac acgcccgtcg tcgattacct ggtgaagccg attccggcgg
    19201 cgacgctgca ggagctgctg ctcggcgcgc acgatcgggc ggtcgcccgc agccgcgtgt
    19261 cgcgtccgct gggcgacatg gtgggcggca gcatccagat gcggaccttg ttcgaccaga
    19321 tccggcaggt cgggccgctc gacgtgtcgg tgctgatcca cggcgagagc ggcaccggca
    19381 aggaactggt cgcgcgcgcg ctgcacgagc agagcgggcg cgccggccgt ttcgtacccg
    19441 tcaattgcgg ggccatcacc cacgacctgc tcggcagcca ggtgttcggc cacgaacgtg
    19501 gtgcattcac cggcgcggtc cagtcccaca ccggcttctt cgaacaggcc gacggcggca
    19561 cgttgttcct ggacgaaatc accgaaatgc cggcgcagct ccaggtgtac ctgctgcgcg
    19621 tgctcgaatc gcggatgctg acccgtctgg gtggcacccg cgaattcccc gtggacgtga
    19681 gactggtggc ggccaccaac cgcgacccgc gcgcggcggt ggcggcgggc gtgctgcggc
    19741 aggacctgta ctaccggctg atagagtttc cgctctacat ccaccccctg cgggaacgcc
    19801 gcgaggacat cgcgctcctg gcgcgccatt tcctggatcg gctcaaccgc aagtacggca
    19861 cctccaagac cttccagccg gatgccctgg tgcggatgac ggaagcggcg tggcctggca
    19921 acgtgcgtga actccgccat gccgtgcagc gccatttcat cctgtcaggc gactccccgc
    19981 aggtcggttt ccggcacgac gacgcaccgg tgaccgcgga atccgacgcg gactccgtgc
    20041 acttccgggt cggcatgacc ttcgaggaag tcgagcgcga aatgttgctg aagacgctgt
    20101 cgcgctgcgg caacaacaag agccgtactg ccaacgtgct gggcatcacc gccaagacca
    20161 tctacaaccg cctgctgcgt taccgggccg aaggcctgat cgacgacagc ctgctgcacg
    20221 gcggccccgc ggaacacgac agcgcaggct gacgcgcgct gcttgtctcg gccgaccggg
    20281 tattcatcgg gccggggacg acgcgcagtt gcgtgcgcgg atgattccga tccgcagcga
    20341 tgcggcgttc ggagttccgt ccttccgtgg cggtgggaac gcgtcctccc gtgcagcaaa
    20401 cacacaagcc cgccggcgcc gcgtgcccat ggagtgggga gccaccaacg ggaaaaatcg
    20461 ggtgagacaa gctggaaata ttttcgtggc cgacgaaggc actgcggggc ggtcggtggc
    20521 acccggcggg ttgcggctga ccggtccgtt ggcccgtgcc atgcaaggct tgctggacct
    20581 cgagcggcgt accgcgccgc gatgtaccat cggatgtcgg gcccgaggcc ggacaccccc
    20641 agcgccggcc gtgggctggc tgcaggcaag caacagaagg gaggatcctg acgtgccggt
    20701 ttccatattg ccggacagcg aagcggaagt ggctgatcgt gatgccgcga tactgccgga
    20761 acgcgatttc aacgccacct tcggcgagat gacgcacgcc gtgctgaatg ccttgaacgc
    20821 ggtcgcggcc gccgccgaac tgtccagact gttgctgagg cgcgacgagc ccgccgaggc
    20881 ctccaaatcc ctggcgcggg tcgagcccga gtgcctgcgt gcggcccgcc tgctgcgcga
    20941 ggggcgcacg ttcctgacct tccgcctcgc cgcgccgggc gaagtcgata tcgccgcgct
    21001 gctgaacgag tgcgtggaaa ggtttgcagg ccgcgcggaa atcgttggcg gagcggggtt
    21061 gccggcgctt cgcggcgatc ccgaggcatt gcgcaagctg ttcaccgagc tgctggaaaa
    21121 cgccttcgcg ttcggcgcca cccatgtccg cgtcaccccg acggaaatcg gaagcgatgc
    21181 ggtccggatc gatttcctcg atgacggccc cggaatcgca atgccggccg cgcgggcgtt
    21241 caccccgttc ttcagtaccc aacccgctca acacagcgga ctggggcttc cgctggcaag
    21301 gcggatcgcc gaggcgcacg gcgggcgcat cgggatcggc gaaagcgagg gcggcgcgtt
    21361 gctctggatc gaactgcccc tgcccgaagc ctgatgcatg cgcacgcagg ctacgctttg
    21421 gcaaaagaag aactgcttcg ccgcgcgtca agggtctcgt gacgcacctg cgaaaaggac
    21481 ttcgatcccg cttgcgctgc gcaggcggcg atcagcgggc gaggaatagt tcgccgatcg
    21541 ccaatgccag cgggtagggc tggaactggt tgcggttgct caggatcacg acgctgaggt
    21601 gctgttccgg ccagcggatg atcacgttcc ggaagcccat ggtttccccc gagtgccatg
    21661 tcgtgccgcc actcagccgc cagccgaatc cgtacaggac gtctggatca tcgaccggat
    21721 ccttgggggt gaacgccagc ttgcgcgatt cggcgtcgag cagcttgtcg gtgtaaagcg
    21781 ccgcgtccca tttcgccatg tcggagacat tcgaatagac gccgccgtcg ccccgggtgg
    21841 cgctggttac gctctggtcg gtgcgcgtcc acttgtcagc gatgaggctg tagccgtagg
    21901 cgcggttggc cggcttcggt ccgcggcggt gttcgtacag caaggtgtgg tccatgccca
    21961 gcggctcgaa gatgcgccga cgcatgaagt cggcgagatc catgcccgac aggcgctcga
    22021 cgatcagccc cagcagcacg tagcccccgt tgctgtagcg gtgcgcgctg cccggctgga
    22081 aatacaggcg atcctgggat gcgatcatgc ggagcacgtc gttgtcgtcg atctgggtgg
    22141 tccggctcga tgggatcagg tcttcgtaat ccaccaggcc gctgctgtgg gccagcaggt
    22201 tttcgatcgt gatgccgtcg tcgctggtgg gcagttccgg caggtacttc cggatcggat
    22261 cgtcgagttc cagcttgccg tcctgcttca gcaccaggat gctcgccgcg gtgaactgct
    22321 tggtgaccga ggcgagccgg tagttggttt cggggccggc cttgatgtgt ttttcgaggt
    22381 tggcgtagcc gtagccgcgc tcgacgaggg gcttgccatc tttcaccacc agaagcgaag
    22441 cacccggcac gtcgcccgcg tagcggctca tcagcgcgtc gatgcgagcc tgatgtgccg
    22501 acgcttgcgc gtgcacggtc gctgcgaccg acaatgcgag tgcgaacagg acaaatggtt
    22561 tgcatgtcat ggtcgaaccc ccgctttcac tgcaccggaa ggtcgtaaag cgtggtcggc
    22621 tgcggatgca tggtgtaggt gtaatgccac cactccgcgg agtagttttc gaaaccctgc
    22681 gcggccatcg cgtgcagcag gcgttggcga ttggcgtgtt gcgatgccgt cgcctgcggc
    22741 gaatcggtgt gggcgagcgg gtcgaacaga tcgaagggcg tccccatgtc cagcggtttg
    22801 cagttcctgt cccgggcgtc acattgcatc atggtcaggt ccatcgttgc gccgcgactg
    22861 tggccggaca ccggcgcgat gtattcgccc agtagcttgg ccttgtcgac gttcggataa
    22921 tgcgccgcct tgctgtcggc gtcgccgtcg tgcgcccagc gcacgaatgc ggcgaccgcg
    22981 cgggcagggc ggtagcagtc ccagatcttc aggcgcgcat gttgctcgcg cagcgcgtgc
    23041 tcgacgcgcg ccagcgcttc ggcggccggc ttcagcagga agcatttggc ggcacggtaa
    23101 ccgggcaccg gcgcgccggt gaaattgtcg ctgccggcat agtggatgtt ttcggcgatg
    23161 tcgggcacca gcgagcggat gtcgaccatg ccggcgtcgg ccatcgtttt cgccggcgag
    23221 attcggggtg gcgcgtcggc gcgcgcaacg gtggccatcg ccaggagagc gatggcggga
    23281 acgcatgcag cgtgcctagt cacacgaacc ctcccgcttg aagcccaggt cctcgtagtc
    23341 gtaactgaaa tcggcgtcgg ggtcgacctt ggccatgcgc agcgtgccgc ccgcttgcgc
    23401 gggaaagcgc agccaggcct cggcgtcgcc gtggttccag tgcacgaggt acttgtcgcc
    23461 gacgcgcatc acctcgccgg tgagcatcgg cgatttcgcg gaagcaaatt ccacggctgt
    23521 tcgcgcggcg cagatatgca cctcgccgaa ccatggatcg cgccacacgc cgagttcgtt
    23581 cttcaggtcg gctggcgtcg cgggttgccg ggaagacgta tcgggtatgc gcgaaatgcg
    23641 ccgctgctcg tcggcccgct tcagttcgtc ggcgtagctc tcgacgctgc gggccctgcc
    23701 gggggcggtg aagtgcttgg ccagcgcctc gttgaacacg ctgcgcgcgt tcccggcttc
    23761 ggcgttgatc atgaagacgt agccgctctg cttgaacggc aacagcgtga ccgccgaata
    23821 catgccagac agcgtgccgg tgtgcgacac ggtcatctcg ccgtcgacgt cgccgatccg
    23881 ccagccgtaa ccatagcccc agaacagcgt gccgtcccat tcgtgccgga gcttcgagat
    23941 cggcatcggc gtgtagggcg tccattccgc atgccgttgc ttcggcgtca gccattgcaa
    24001 ctgctgcggc gtgggtgcca gccagttcat cgcccaggtc agcatgtcgt ggagcccgca
    24061 tcgcaccccg ccggccgaat ccatggtggt ttcgtgcacg atcgcgccgc gatggcggct
    24121 cgcgacaaag tggccgtttt cgatggtgtg cgggtccgcc acgttgccga cttcgtcgcg
    24181 gttccaggtg ccgatctggc agcgcgtcat gccgagcggc tggaagattt cactgcgcat
    24241 cagcgtcgcg tatggtgcgc cgccggctgc cgccgcgacc tggccggcca cgatgtacag
    24301 cgtgttgtcg taggcgtaac ccgcgcgaaa actgtaggcc ggcttgatgt accgcaggcc
    24361 tgcgaccacg tccgcgggcg tgaaatggtt gggcgtcggc cacagcatca ggtcgcccgc
    24421 gccttcggat agcccgctgt ggtgcaccag caagtcgccg acctgcatgt gcttcgtcac
    24481 ccacgggtcg tgcatccgga acgacggcag gtatttcgtg acggggtcgt cccagcgcaa
    24541 cttgccctgc tgcaccagcc gcgccagcag ggtggaagtc atggccttgg tgttggacgc
    24601 gatttcgaac agcgtctcgt cgtcgatcgg cttgcccgat gcgagctttc ccacctcgcg
    24661 ggtgtagacc accttgccgt ggtcgatcac gccgaccgcg agaccgggca ggtggtagta
    24721 cgcgaccacc gcatcgacga ggcgatcgat gtcggcgttg tggttggctg gcggagtggc
    24781 ggcgtgcgca atggccgcgt acccagccaa cgcaaccatg ccgaagaacc atttgttggt
    24841 gcgaaagttc atccggaaac ctgcacacgg ttcgtgttga gcgtagtgga gcgcagctcc
    24901 acgaagacga aacactgggc ttgcgtgatg cgggccttcg actccgcgcc tgcggccctg
    24961 cgctcggagc ccgccctgga cttgatccgg gggggaaccg atatctggcg tgcgccgctc
    25021 atgcgtgctc ccactgttgc gctgcttgct ctcccgcaat catctcctcg agcgtctggc
    25081 gccgccgcac cacggcgtag ctaccacgat ccagcaacac ttccgcggcc agcggccgtg
    25141 agttgtaggt cgatgccatg gacgcgccgt aggcgcccgt ggccttgatc atcaccaggt
    25201 cgccggcgcg gcattccggc agcgtgcgat cgcgcgcgaa ggtgtcgccg gtttcgcaga
    25261 ctggtcccac gatgtcgtag gccagcaacg ggcgtgaccc atccaccagc ggtacgatgt
    25321 cgtgccatgc gtcgtacagg gaaggccgga tcagctcggt catcgcggca tcgagcacca
    25381 ggaatgtgcg ccgctgaccg cgcttgacgc ggatcacgcg ggtcagcagc acacccgctt
    25441 cggccaccag ccagcggccg ggttccagca ggatgcggcc gtcgaaatcc gccagcgctc
    25501 cccgaatcgc atcggcgtag tcgtcgattg caacgggatg atcctggtct gcgcgatagc
    25561 acacgcccag gccaccaccg acatcgatgc tgcggatcgc gtgtcccgca gtgcccagtt
    25621 cgcgccaaag ctccgccacg cgccgcaggg ccgcgcggat cggatcgagg cccagcattt
    25681 gggagccgat gtggacgtgc aggccgtcca gccgtacgtg ggtcagcgcc gcgctgttcg
    25741 caaaccactc gcgcgcctcc tcgacgccga ccccgaactt gttctccgat tttcccgtgg
    25801 caatcttggc gtgggtgcgg gcgtccacgt tggggttgat gcgcgcggaa gcatgcgcgg
    25861 tgatgccccg tacggccgcg agccgttgca gcagttgcag ttcgtccgcc gactcgacgt
    25921 tgaagcgcgc cacgcccgct tccagcgcgt ccgcgatttc ctccacggtc ttgccaacgc
    25981 cggaaaacac gatgtgttcc gcgggaatgc cggcgcgcag gcagcgccac aattcgccgc
    26041 ccgagacgat gtcggcgcca aagcccgctg tcgccatcag ttgcaggatc gcgatgttcg
    26101 aattcgcctt ggccgcgtag cagacccgtg cgtccaggcc ccgcaacgcc ctttgcagtg
    26161 cgtcgacgcg cccgcggatc acgctcgcgg aataaacctg gcacggcgtg cccacgcgcc
    26221 gtgccagagc ggggagttcg acgccgtcca ggcatgctgc ctcagggcat gcaaggtcgg
    26281 cgtgggcagt cgttgcggtc atcgcctgcg attgcttcga ttggaaatgg ttgccggtat
    26341 tgaagctgcc aatggcctac ggacggcgag tagtcagtag cccgccgcga gcccggcgga
    26401 gcggtgggga tcgctcgcgc cttccagcat cccggtttcg ggattgacga ggatggattc
    26461 gagtacgccg aggtggcgtt ccttgagggt gtaccccatc gcttcgagtt gccgtttcga
    26521 ttccggcgtc agcatgccgg gtccgacgta cacgacatcc gggtaccact gcatgtggac
    26581 atgtggcgcg ttcacggcct gttgcatgtt catgccgaag tcgaccacgt tgatgatgct
    26641 ctgcatcgtg gtcgaaatga tggtggcgct gcccgggctg ccgctcagca tgaacggctt
    26701 gccggccttc agcacgaccg tcggcgtcat cgaactcaag ggacgcttgc cgggctcgac
    26761 ctggtcggcc tccccctgca cgagtccggc gccgttggac gcgcccggct tggcggtgaa
    26821 atcgtccatc tcgtcgttga ggtagaagcc ggtatcgcct gcgatctgct tgatgccgaa
    26881 caggaagttg atggtgtagg tgacggccac ggcattgccg tgcccgtcca ttaccgaata
    26941 atgcgtggtg ttgttgccct cgctgggggg caggaagctc ttgatctcgg acgacggcgt
    27001 agccttgtcg ggttcgatcg tcgcgcgcag cgacgcggcg tactccgccg acatcagctt
    27061 gtccatcggg atgtcgacga aatccggatc gccgaggtag gtgttgcggg tcgcaaaggc
    27121 gcggcgctcg gcttcggcga agtaatgcac gaaaggcacc gaagcgtagc cccagtccgc
    27181 gaccggatag ggcttcagga tgttcagcgc caggcacatg gtggtgccgc cggagctcgg
    27241 cggtggcgcc gaaaggatgg tgtagccgcg gtattcgcat tgcagcggct tcatccattc
    27301 cacgttgtag ctggcgaaat ccttcatgcc gaggatgccg ccgtgggcgt cactggcttt
    27361 caccaccgcc cttgcgatcg agcccttgta gaacgcatcg gtgccgccct tggcgatctt
    27421 ctccagggtg ttggcgaggt ccttttgcac cacgcggtcg ccggctttcc acggcttgcc
    27481 gccgttgccg aagaccgctg cggattccgg atacttgttg agcgcttcca gatcattgtt
    27541 gatgatctcg gcgtcgcctt cctccagcac gaacccgtcg cgcgccagct cgatcgcggg
    27601 ctggatgacc tgcttcaggc tcatcgtgcc gtatttttcg agcgcggtat ccaggcccat
    27661 cacggtgccg ggcacgccca cgccgagata ggtgtcggtg ctgcggcctt cgacgacctt
    27721 cccctgcgcg tccaggaaca tcgtgggcgt ggccttcagc ggcgccttct cgcggaagtt
    27781 caggaacagg ttctcgccgt tggcgaggtg gatcgtcatg aagccaccgc cgccgatgtt
    27841 gccgcagcac ggttgcgtca ccgccagcgc atagcccacc gcgaccgccg catcgatcgc
    27901 gttgccgccc tccttcagga ttttcacgcc agccttggtc gcgaggtgtt ggtcggagac
    27961 gaccattgca tgctgggcga cgaccggttt gacgttctca agtgcggcgc gcgtgtcggc
    28021 gggaagatgc acgaacggtg aagtggtggc cgtggcggct gccggcgccg tgctggcgga
    28081 agccacacgg ctggcatgcg tcgtgtcgtt ggtgctgcag ccggcaatgc cgaacgcgaa
    28141 caaacccgct gccagcaacg acagccggcg gtattgccat cgccggcggt acgaagcggt
    28201 ggaatgcttg acgcgcgagc gggggagagt tttgcctggc atgttcagtt cctgattgac
    28261 ttcaagacgt tccgaatggg taaaagcgag cttccacgcg gatgaacgcg gataaagcat
    28321 tggatccgtt cttatccgcg ctcatccgcg tcaaagactt ctctttgcag caaagcccat
    28381 cacgccatgg atttcgacgc gcgtccaccc gtcagcttcc ccagaaacca agcgcgggcg
    28441 gcacgagcag gccatcctgc agttccacgc cgcccgagta gtcgttctcc agccataacg
    28501 gtccgtccag gtcgacgaac tcggcttcgc gggcgatttc cagcgcgggc gcgatcccca
    28561 gcgacgagca aaccatgcag ccgaccatga tccggaagcc ttgcgcgcgc gcggcacgca
    28621 gcaggcgcca ggcttcggtc aggccgccgg tcttgtcgag cttgatgttg acggcctggt
    28681 agcggttgcg cagcttcggc aggtcggtcg tgacgtggca ggcctcgtcc gcgcaaacgg
    28741 gaatcctgcc ggaatactcg agcaaggcgt cgtctgcgcc ggcgggcagc ggctgctcca
    28801 gcagggccac cgacgcgcgt tccagtaccg actggacatc gcgcagcacg tcgctcgtcc
    28861 agctttcgtt gggatcgacg atcaggcggg ccgatggggc ggcacgccgc acggcttcga
    28921 tccgcgcggc gggatcgccg gcgtcgacct tgatcttgat cagcttcgcg tccgcgatcc
    28981 gcgcggctgc ggcttccatg gcttggggcg cgtcgaggct gacggtgagc gcggtgacga
    29041 gcggtacatg cgacggcctc gccaaacgcg cccagacgga cacgccggtg agtttcgatt
    29101 ccaggtccca cag
//
LOCUS       DASSNH010000319         6772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1616423, whole genome shotgun sequence.
ACCESSION   DASSNH010000319 DASSNH010000000
VERSION     DASSNH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616423"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFK00_12065"
     CDS             <1..358
                     /locus_tag="VFK00_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HET7593723.1"
                     /translation="AAGEDLSWFWRGWFRHNWKLDLAVIGVKYVDDDPAKGALVTVAN
                     LDKFAMPAMLRVTHDDGSTTQIRVPVATWIQHTQFDAPVPGNKPIRSATIDPDGELPD
                     IDRANNTFAAGAARTR"
     gene            435..1988
                     /locus_tag="VFK00_12070"
     CDS             435..1988
                     /locus_tag="VFK00_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7593724.1"
                     /translation="MRTRTWLSLLLALVAPFAFASPPAAAAAPADVPGLDAYVQQVMH
                     DWHVPGLSIAVVKEGKVLLARGYGVRRLDEPGKVDADTLFDIGSNTKAFTVAALGTLV
                     SGGKLQWDTPVVDELKEWRLQDPYVTQRATLLDLLTHRTGYCDPGAVWYTSDASDILE
                     RVRYQEPKYGFRTTFCYNNVQYLAAGRFIPAITGGSWNDYVAAHLLRPLDMDRTVTTD
                     AAVEASGNVAAPHGMVDGSPAAVGRYWPHNMDAFAAVGGIWSSANDMSHWLQMLLANG
                     KYAGRHVLDAQVIDAMETPRILIQSDGIGGEIRRWMPGGHFYTYGLGLFVQDYHGHLL
                     VWHAGDIDGMASAMALVPDARLGIVVLSNMDHADARFAIVARVLQGMLELPTRDIEAT
                     LLAATHKNDAEGDAIEKKLADTRVAGNAPPLPLADYAGTYTDELDGEAQVAVEHGHLV
                     LRLGNPDFTGALTPWHGNTFRVDWRHQFYGDDYVTFDVDALGRPSKLHLAGASLHYQR
                     SQSAAPKPN"
     gene            2172..3584
                     /locus_tag="VFK00_12075"
     CDS             2172..3584
                     /locus_tag="VFK00_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7593725.1"
                     /translation="MQHAKDRPHWKRTLAILGVMAATFAFQETGVLPVLPVIQRQLAG
                     ASTTSSALLESGFLIVAAVAAPLIGKYGDAHGKQRMLLVTLAIYLIGAIGAGFAPNFI
                     VLVVFRALQGAGGALFSLSFAVMRDEAPGRLSVAIGVLVGSFGIGSSLGFGLSGVITE
                     LLSWRWIFFAEAVLIVAVAALVALLVPRSPDRHDVSIDYQGVAWLGAALAILVISLTF
                     MLVLGWKVVGGFALFAVLFAGWIWREQRAEQPLLDIGILTKPQVLLPNLGSGLAGYVA
                     FSTFFLVPRFVQVPRHLAPHVAHHMQYGFGAGPTAIGLFLLPVGVGVLWAGPVGGILG
                     RRIGGKWPFVAGLALLVAGSALLATAHDERHAFAVWLFLVGTGFGLSIGAAGVFVTQA
                     VGEGQTGIANAFNSLTRLVVGGIGAQVAAAIMKSQHVSGSSAPHESAFAIAFGICAVL
                     AAIGCGLALIVPVDEPPSPS"
     gene            3673..4350
                     /locus_tag="VFK00_12080"
     CDS             3673..4350
                     /locus_tag="VFK00_12080"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET7593726.1"
                     /translation="MNKESTATAGQSDLTPRVDTLCSACGGIALVGVAAITVICLGVQ
                     FLRSDLDWITTAMSLYVKGPWGGFVQASFFAPAPGLAAIGYAWHRTLLRREPGSMASF
                     ALFVAAAAALCLTGAFVTDPTPSPTTLHGTIHQWAAFGTFVFVTTAMVLQSWWFRYDP
                     HWRKHFADAFAIAMGTIVYFWIYALVKPIPRGIGEKVVIALVLLWVWRAAWWLVRDRS
                     CVDRRSR"
     gene            4409..5416
                     /locus_tag="VFK00_12085"
     CDS             4409..5416
                     /locus_tag="VFK00_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET7593727.1"
                     /translation="MADAPLATPTPADIQRARERLGDRVRETPTWQWRGDAVARAAGA
                     DTQVFLKLELFQYTGTFKARGALLNAMALDADARARGVTAVSAGNHAIAVAFAARSVG
                     TSAKVVMPRTANPARVALCRAYGAEVLLMDDVYAAFDEVERIRRDEGRTFIHPFEGET
                     TVLGTATVGHELCHQVEHLDAVIVPIGGGGLIAGIACAVKQLQPRCQVFGVEPEGADS
                     MSRSFEAGSPQKLERVATIADSLAAPYALPYSFGVARQFVDEIVTIPDDAMRRAMGLL
                     FADMKLAVEPAGAAATAALCGPLRERLSGQRVGVIVCGANIDAATFAAQMIPCEPGLN
                     A"
     gene            complement(5454..>6772)
                     /locus_tag="VFK00_12090"
     CDS             complement(5454..>6772)
                     /locus_tag="VFK00_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HET7593728.1"
                     /translation="WFELMGIAAFALTAYPLGKSSLEGAFNFTVTNALGSFLMLAGVG
                     LLYARTGTLDFVTMGRVIASNPPDPVVAGGFCLVATALLTKAAIVPFHMWLSDAHAVA
                     PSPVSVIFSGVMVSLGLFGLLKLIAIVFAGDPRVMTLVHVGLFWLGVTTAVVGGLMAW
                     AQRHLKRLLAFSTIAHLGIMLIAVASVSAIGTEGFLVYLVGHGLVKGTLFMIAGILLA
                     RFNSVDEIELYGEGRSLWPAGIAMGLAGLLLGGLPWGLLHDATDSVHAGSHGVAGALA
                     IIVGTALTGAAVLRATGRIFLRWSGAPAVERIAPTEREREKGDRPLWLMLLPCVVMLA
                     IAVLPTSLAESFVHSAATGLVSRVIPHEGGGTLAAAITFALTILIPAASLLRGRAMSR
                     GARLAAEAEMAPFRALQVLHSGRVTDYVVWMMVGLAVLAGWLGFGW"
BASE COUNT         1103 a         2329 c         2252 g         1088 t
ORIGIN      
        1 cgccgccggc gaagacctgt cgtggttctg gcgcggctgg ttccggcaca actggaaact
       61 cgacctcgcc gtcatcggcg tgaagtacgt cgacgacgat cccgcgaagg gcgcgctggt
      121 cacggtggcg aacctcgaca aattcgcgat gccggcgatg ctgcgcgtca cgcatgacga
      181 cggcagcacc acgcaaatcc gcgtgccggt cgcgacctgg atccagcaca cgcaattcga
      241 cgcaccggtg ccaggcaaca agcccatccg cagtgcgacg atcgacccgg acggcgagct
      301 gcccgacatc gatcgcgcca acaatacgtt cgctgccggc gccgcgcgca cgcggtgagg
      361 ttgcacgacc gtcgccggac caggtccccg gccgtgccac aatgcgccgg tctttcaccc
      421 gagggacgac gacgatgcgc acacgtacgt ggctttcgct gttgttggcc ctggtggcgc
      481 ctttcgcgtt cgcgtcgcca cccgccgcgg cggcagcgcc cgcggatgtg ccgggcctcg
      541 acgcctacgt gcaacaggtg atgcacgact ggcacgttcc gggcctgtcg atcgcagtgg
      601 tgaaggaggg caaggtgctg ctggcgcgcg gctacggcgt gcgccggctg gacgagccgg
      661 gcaaggttga cgccgacacg ctgttcgaca tcggctcgaa caccaaggca ttcacggtgg
      721 ccgcgctcgg cacgctggtg tccggcggca agttgcagtg ggacacgccg gtggtcgacg
      781 agctgaagga atggcgcctg caggacccgt acgtcacgca gcgcgccacg ttgctcgacc
      841 tgctgacgca ccggaccggc tattgcgatc cgggcgcggt gtggtacacc agcgacgcgt
      901 ccgacatcct cgagcgtgtg cgttaccagg aaccgaaata cggttttcgc accacgttct
      961 gctacaacaa tgtccagtat ctcgcggccg gccgtttcat tccggcgatc accggcggga
     1021 gctggaacga ctacgtcgcc gcccacctgc tccggccgct cgacatggac cggacggtga
     1081 ccaccgatgc cgcggtcgaa gcgtcgggca atgtcgccgc gccgcacggg atggtggacg
     1141 ggagcccggc ggcggtcggc cgctactggc cgcacaacat ggacgcgttc gccgcggtgg
     1201 gcggcatctg gtccagcgcg aatgacatga gccactggct gcagatgctg ctcgccaacg
     1261 gcaagtacgc cgggcgccac gtgctggatg cccaggtgat cgatgcgatg gaaacgccga
     1321 ggatcctgat ccagtccgac gggatcggcg gggagatccg gcgctggatg ccgggcggcc
     1381 atttctacac ctacgggctg gggctgttcg tgcaggatta ccacgggcac ctgctggtgt
     1441 ggcatgcggg cgacatcgat ggcatggcgt cggcgatggc gctggtgccg gacgcgcggc
     1501 tcgggatcgt cgtgttgtcc aacatggatc atgccgacgc tcgttttgcg atcgtcgccc
     1561 gggtcttgca aggcatgctc gaactgccaa cgcgcgacat cgaggcgacg ctgctggccg
     1621 ccacgcacaa gaacgacgcg gagggcgacg cgatcgaaaa gaagctcgcc gacacgcgtg
     1681 tcgccggcaa cgcgccgccg ctgccgctgg ccgactacgc cgggacgtac acggacgaac
     1741 tggatggcga ggcacaggtc gcggtcgagc acggccatct ggtgttgcgc ctaggcaacc
     1801 ccgatttcac cggcgcacta acgccctggc acggcaatac cttccgcgtg gattggcgcc
     1861 accagttcta cggcgacgac tacgtgacct tcgacgtcga tgcattgggc aggccgtcga
     1921 agcttcacct cgccggcgca tcactgcact accagcgcag ccaatcggcc gcccccaaac
     1981 cgaattgacg gcacgcgagt acgcacgaaa aaaaggcggc catcgagaga cgtaacgcat
     2041 cgggcggtgt ctccgctacg tgcaggagcg cgccatgcgc gcgaactgtc cccgcgcgag
     2101 tgtcggcgca cgtccacttc gccgacggcg cattcagccg ccgcgcacct tgcgccgcgt
     2161 agcatgggtc gatgcagcac gccaaggatc ggccgcactg gaagcgtacg ctcgccatcc
     2221 tcggggtgat ggcggccacc ttcgccttcc aggaaaccgg cgtcctgccg gtgcttcctg
     2281 tgatccagcg gcaactggcc ggcgccagca ccacgtccag cgccctgctc gaaagcggct
     2341 tcctgatcgt ggcagcggtc gcggcgcccc tgatcgggaa gtacggcgac gcgcatggca
     2401 agcagcgcat gctgctggtg acgctggcca tctatttgat cggcgcgatc ggcgcgggct
     2461 ttgcaccgaa cttcatcgtg ctggtggtct tccgggcatt gcagggcgcg ggcggcgcgt
     2521 tgttctcgct gagcttcgcg gtgatgcgtg acgaggcccc gggacggctg agcgtcgcga
     2581 tcggcgtgct ggtcggttcg ttcggcatcg gctcgagcct gggcttcggc ctttccggcg
     2641 tgatcacgga gctgctctcc tggcgctgga tcttcttcgc cgaagccgtc ctgattgtgg
     2701 ccgtggcggc gctggtcgcg ctgttggtgc cgcgctcgcc cgatcgccac gacgtgtcga
     2761 tcgactacca gggcgtggca tggctcggcg ccgcgctcgc gattctcgtg atctcgctga
     2821 cgttcatgct ggtgctgggc tggaaagtgg tgggcgggtt cgcattgttc gcggtgctgt
     2881 tcgccggctg gatatggcgg gagcaaaggg ccgagcaacc cctgctcgac atcggcatcc
     2941 tcaccaagcc gcaggtgctg cttccgaacc ttggatcggg cctcgccggc tatgtcgcgt
     3001 tcagcacgtt cttcctggtg ccgcgtttcg tgcaggtgcc gcgccacctc gcgccgcacg
     3061 tcgcgcacca catgcagtac ggcttcggcg ccggaccgac ggcgatcggt ctgttcctgc
     3121 tgccggtcgg cgtcggcgtg ctgtgggcgg ggccggtcgg cggaatcctc ggacggcgca
     3181 tcggcggcaa atggccgttc gtggccgggc tcgcgctgct ggtggctgga tcggcgctcc
     3241 tcgcgacggc gcacgacgaa cgccacgcgt ttgccgtctg gctgttcctg gtcgggaccg
     3301 gtttcggttt gtcgatcggc gcggccggcg tgttcgtgac ccaggcggtt ggcgagggcc
     3361 agacgggcat cgccaacgcc ttcaacagcc tcacgcggct ggtggtgggc gggatcggcg
     3421 cgcaggtcgc agcggcaatc atgaagtcgc agcacgtgtc cgggtccagc gcgccgcacg
     3481 agtcggcgtt cgcgatcgcc ttcggtatct gcgcggtgct ggcggcgatc ggctgcggac
     3541 tcgcattgat cgttcctgtg gacgagcccc cttccccttc gtgaggcatc ggcaaccgcg
     3601 cacgggccgc ctccacttca cggcgcaggc acgtggtatg cgccatattt gcccgagcat
     3661 ggacgcaaca tgatgaacaa ggaatccacg gccactgccg gccaatccga tttgaccccc
     3721 cgcgtcgaca ccctttgcag cgcgtgcggg ggcatcgcgc tggtcggcgt ggccgcgatc
     3781 accgtgatct gcctgggggt gcagttcctc cgcagcgacc tcgactggat caccacggcg
     3841 atgagcctct acgtcaaggg accgtggggc ggattcgtgc aggcgagctt ctttgccccg
     3901 gcgccgggcc tcgccgccat cggctatgcc tggcaccgga cgctgctgcg ccgggagccc
     3961 ggaagcatgg cgtcgttcgc gttgttcgtg gcagcggccg ccgccctctg cctgaccggc
     4021 gccttcgtca ccgatccgac gccgtcgccc acgacattgc acggcacgat ccaccaatgg
     4081 gcggcattcg gaaccttcgt gttcgtgacg accgcgatgg tgctgcaatc gtggtggttc
     4141 cgctacgacc cgcattggcg caagcacttc gcggatgcgt ttgccatcgc gatgggcacg
     4201 atcgtgtatt tctggattta cgcgctggtc aagccgattc cgcgcggcat cggcgaaaaa
     4261 gtcgtgatcg cgctggtgtt gctgtgggtg tggcgcgccg cgtggtggct ggtgcgcgat
     4321 cgttcctgcg tggaccggcg ttcccgctga ccgctcggtc gaacggcgat acgatgggtc
     4381 gtccgccgcc gaagtccgcg accccgccat ggccgatgcc ccgctcgcaa ccccaacacc
     4441 cgccgacatc cagcgcgcgc gggaacggct cggcgatcgc gtccgggaga cgccgacgtg
     4501 gcaatggcgg ggcgatgccg ttgcccgagc cgccggtgcc gatacccagg tattcctgaa
     4561 gctcgaactg ttccagtaca ccggtacgtt caaggcacgc ggtgcgctgc tgaatgcaat
     4621 ggcgctggat gccgatgcac gcgcgcgcgg cgtcaccgcg gtgagcgccg gcaaccatgc
     4681 catcgcggtc gcattcgcgg cgcgcagcgt cggcacctcc gcgaaagtcg tgatgcccag
     4741 gaccgccaat cccgcgcggg tggcgctgtg ccgcgcctac ggtgccgaag tcctgctgat
     4801 ggatgacgtg tatgcggcct tcgacgaggt cgaacgcatc cgtcgtgacg agggccgcac
     4861 cttcatccat ccgttcgaag gcgaaacgac cgtgctcggc accgcgaccg tcggccacga
     4921 actatgccat caggtcgaac acctcgatgc cgtgatcgtc ccgatcggcg gcggcggcct
     4981 gatcgcgggg atcgcctgcg cggtgaaaca actgcaacca cggtgccagg tgttcggcgt
     5041 cgaacccgaa ggcgccgaca gcatgtcgcg cagcttcgag gccggttcgc cgcagaaact
     5101 cgagcgcgtc gcgacgattg ccgacagcct cgcggcgcct tatgcgctcc cctacagctt
     5161 cggcgtcgcc aggcagtttg tcgatgaaat cgtgacaatc ccggacgatg cgatgcgccg
     5221 cgcgatgggg ctcctgttcg cggacatgaa acttgcggtg gaaccggccg gtgccgccgc
     5281 caccgccgcg ctgtgcggcc cgctgcgcga gcggctctcc ggtcaacgcg tcggcgtgat
     5341 cgtgtgcggc gccaacatcg acgcggcaac gttcgcagcg cagatgatcc cctgcgagcc
     5401 cggcctgaac gcgtaacccc cagggtgcgc catggccgcg ctcgagcgga ccgtcaccag
     5461 ccgaatccga gccaaccggc cagcaccgcc agccccacca tcatccacac cacgtagtcg
     5521 gtcacgcgtc cgctgtgcag gacctgcagc gcacggaaag gcgccatttc cgcttccgca
     5581 gccaggcgtg cgcccctgct catcgctcgc ccgcgcagga gcgacgcggc ggggatcagg
     5641 atcgtcaacg cgaaggtgat cgccgccgcc agcgtgccgc cgccttcatg cggtattacg
     5701 cgcgacacca gtcccgtcgc cgcggaatgc acgaaggatt ccgcaaggct cgtcggcagc
     5761 accgcgatcg ccagcatcac cacgcacggc aacagcatca gccacagcgg ccgatcgccc
     5821 ttctcgcgct cgcgctcggt aggtgcgatc cgctcgaccg ccggcgcccc gctccagcgg
     5881 aggaaaatgc ggccggtggc gcgcagcacc gccgcaccgg tcagcgcggt gccgacgatg
     5941 atcgccagtg ccccggctac gccgtggcta ccggcatgca ccgagtccgt cgcgtcgtgc
     6001 agcagccccc acggaagacc ccccagcagc agcccggcga gccccatcgc gatgccggcc
     6061 ggccataagc tccgcccctc gccgtacaac tcgatttcat ccaccgaatt gaaccgcgcg
     6121 agcaggatcc ccgcaatcat gaacagggtt cccttcacca gcccatggcc aacgaggtac
     6181 acgaggaacc cttcggtgcc gatcgcactg acggacgcga ccgcgatcag catgattccg
     6241 aggtgggcta tggtcgagaa cgccagcagc cgcttcaggt gccgttgcgc ccacgccatc
     6301 aggccaccca ccaccgcggt cgtgacgccg agccagaaca gtccgacatg caccagcgtc
     6361 atcacccgcg gatcgccggc aaagacgatc gcaatcagct tcaacaaccc gaacaagccg
     6421 aggctcacca tcaccccgga aaagatcacc gacaccgggc tcggcgcgac cgcgtgggcg
     6481 tcggacagcc acatgtggaa tggcacgatc gcagccttcg tcagcaaagc cgtcgcgacc
     6541 aggcaaaagc cacccgccac caccgggtcg ggcggattcg acgcgatgac gcgccccatg
     6601 gtcacgaagt cgagggtgcc cgtccgcgcg taaagcaggc cgacgccggc gagcatcagg
     6661 aaactgccaa gcgcgttggt gacggtgaaa ttgaacgcgc cctccagcga actcttgcca
     6721 agcggatacg cggtcagcgc aaatgccgcg atccccatca gctcgaacca ga
//
LOCUS       DASSNH010000320        12773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_886713, whole genome shotgun sequence.
ACCESSION   DASSNH010000320 DASSNH010000000
VERSION     DASSNH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886713"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            604..1440
                     /locus_tag="VFK00_12095"
     CDS             604..1440
                     /locus_tag="VFK00_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7593729.1"
                     /translation="MRRAILTTGALFLIGCISTPAMAGFMAANDGFFSYTGTVTAPDG
                     HTYTIPAYTSTPNGTSYVGRDASVYVTSNAPTVDTGAGYENSTVFETNWYSGTSNPNN
                     TDTGFVQLSDLASQGVTSVSGGWTDNSYTTFTFTVVGNAGSGDPTYDRLWAAPRVGGP
                     ATDTAGAFGGYSLVLTATFAPGAVTEESPGWFSTDATPLSITGSFSGLFTNTGTYAPG
                     LYQFDFDFLDQSWAGNNGVGGPSYFGASAVAVPEPATLGLFGFGLMALGLCVRMRRRS
                     LA"
     gene            complement(1506..2468)
                     /locus_tag="VFK00_12100"
     CDS             complement(1506..2468)
                     /locus_tag="VFK00_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7593730.1"
                     /translation="MADFIAPTLADIRAAAARIAAHARVTPVLSDETLDGMAGAAMFF
                     KCEHLQRGGAFKFRGACNAVWSLPDAEARCGVVTHSSGNHGAALALAAATRGIPAHVV
                     VPEGAVAAKLANIEGAGGIIHRCAPTQTAREETCARVQRETGATLVHPYADARVVGGQ
                     GTVVLELLQQVPELDAVIAPVGGGGLVSGCAIVVRALQPSSDVVGAEPAGADDAFQSL
                     GRGQRVTDVVPETICDGLRATIGIPNLAMLEQHHVEIVTVDDAATLAAMRLLWKELKQ
                     TVEPSSATVLAAVLAQRERFAGKRVGLVLTGGNVDLEQLPFGPG"
     gene            2669..5359
                     /gene="pepN"
                     /locus_tag="VFK00_12105"
     CDS             2669..5359
                     /gene="pepN"
                     /locus_tag="VFK00_12105"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HET7593731.1"
                     /translation="MDTQTDAGAPATETIVRLADYRPPAWRVTHVDLVFDLGVDETIV
                     RSKLILARDRDEPLHLDGEDVELIEARVDGRKLGETEYVVDQNGLTIADVRDGSTLEL
                     DVRIRPARNSQLSGLYLSGSRDRGFLLTQCEAQGFRRITFFPDRPDVLARYDVTLRAP
                     KARFPVLLAGGEPMESGALDDGRHFARFVDPHPKPSYLFALVAGYLEKIERPYRTADG
                     RDVTLRLWAEDGAIDRCHYAMDALERAMRWDERAYGRNYDLPVFNVVATDDFNMGAME
                     NKGLNIFNSKYLLADPRVTTDDEYRRVEAVIGHEYFHNWSGNRVTCRDWFQLSLKEGF
                     TVFREHSFCEDMHSAALKRIEDVATLRRAQFAEDAGLLAHPVRPAEYQAIDNFYTATV
                     YEKGAELIRMLAGKLGRDGFRRGTDLYFSRFDGQAVTIEDLLAALGEANDADLSPYLA
                     WYAQAGTPEVRASGEYDAPARRYTLTLSQRTPPTPGQPHKQPLPIPVRIALFARAGAP
                     LACRVDGGNPNASTDHVLELDRAEQRFVFTDVASEPTPSLCRGFSAPVRVAFDYSAEQ
                     LALLLGHETEGFDRWNAAQELAGRAFDEILAGQREHPAVDAWLDALARLFAERARSID
                     PALLADLLSLPSAIELGVRHAPFDPDAVHAAREALENALAARLGEALRAAYRELHANE
                     TGSLDQDAAARRRLKARVLALLARFDPEQGAELAFAQYRDARGMTDRLAALATLVRRG
                     APQAADALRLYRESFDGDALTLDKWFSLQATAPVHDMLARVQALEADPAFDLANPNRV
                     HALLGAFARANPVAFHRPDGAGHRWFAGKLAEIDTRNPQLAARLAKAFEDWPRLEPGR
                     RASAEGALRELLARGGHSPDLTEILTRVLAADDRKPGSGA"
     gene            5746..6609
                     /locus_tag="VFK00_12110"
     CDS             5746..6609
                     /locus_tag="VFK00_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593732.1"
                     /translation="MRKTGEMRAVARGARLAVFGAGVLLLAGCATGYSFVQPGVAGSG
                     GYYTGDGPYSGQGYYDYYGTGPYYAGTSGYGYYNGTRPYSSPYGYYGPGYGYGYGYDY
                     GSQWIFNLGISNVWNFPGYWGPWYTTGYASWGCGSWRCGNRHHGHHHDHDNGSWRHDD
                     ASSLPVDVPHTAGNSQPDFNAGRERAMERTAWRHDHYVRSPRQGAAVPNFSAVPAMPS
                     YSGGQAPRATSFGTPAARPAAAPMRMPAPQAASRAPVAAPQPSFRPAMPMRAAPPAAS
                     RTGTNTGTKVQ"
     gene            complement(6696..6899)
                     /locus_tag="VFK00_12115"
     CDS             complement(6696..6899)
                     /locus_tag="VFK00_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593733.1"
                     /translation="MNFIDMTKEEWEEVRRHHDELVQMGRLELEAEGIRECRPAGASP
                     EAANHPVGRRLCELDDMVFGVSH"
     gene            7579..9453
                     /gene="mnmG"
                     /locus_tag="VFK00_12120"
     CDS             7579..9453
                     /gene="mnmG"
                     /locus_tag="VFK00_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7593734.1"
                     /translation="MHRYDVIVVGGGHAGTEAALASARCGARTLLLTHNIETIGQMSC
                     NPAIGGIGKGHLVKEIDALGGAMAHAADRAGIQWRTLNASKGPAVRATRCQADRVLYR
                     AAIRHTVERQPNLELFQQAVDDLVIESGRVVGVVTQMGLTIRARRVVLTVGTFLAGKI
                     HIGLENHSGGRAGDPPATTLAKRLRELPLGADRLKTGTPPRIDARTLDYSVFEEQPGD
                     DPLPVFSYLGSRDEHPRQVPCWITHTSERTHDIIRAGLDRSPLYTGKIEGTGPRYCPS
                     IEDKVVRFADKASHQVFIEPEGLDAIEIYPNGISTSLPFDVQTELVHSIPGFERAHIT
                     RPGYAIEYDYFDPRGLHPWLETKAIGGLHLAGQINGTTGYEEAAAQGLVAGLNAARMA
                     RGGEPWWPRRDEAYIGVLIDDLTTNGTIEPYRMFTSRAEYRLHLREDNADLRLTETGF
                     QLGAVPRERFDAMRAKRDAVERETARLKSLCATPANALGAALQSERGITLSHEATALE
                     LLRRPELGYSMLVATNGFGPAVEREDVAAQVEVQAKYAGYLERQRDEIERQRHHESTS
                     IPDDFDYDRVRGLSAEVSVKLKRVRPATVGQARRISGVTPAAISLLLVHLKRGDSRAA
                     "
     gene            9732..10967
                     /locus_tag="VFK00_12125"
     CDS             9732..10967
                     /locus_tag="VFK00_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET7593735.1"
                     /translation="MIREATDNWTETVYRPLRRVGRRLSHEFATGAPRASRRPERYLA
                     LDNARFVLIALVVVGHLLEQLVNTGPFSDTLYRWIYLFHMPGFILISGALSKRTLTHR
                     RLLGLATGLLVPYVIFQTLYPAWDALLFPGNDWSQGYLTPYWLLWYLPSLVCWRLMLP
                     LFAHRWALPAAVAIALAAGLAPWIGYPFSLSRTLVFFPLFLLGHRIGAQQLQRLGASR
                     GARVCAVVVLAAAGAGAWWLRGLDPQWLYASVGYATLNVDAVPGAAIRLALLAASSAC
                     ALAAIALVPRRPLWAGFGRRSLTTYLMHGFLIRGLLAAGVFAWLSHEIVPPLQVLLCL
                     ACGALIAALFSTRFMDKLATPLTRPVRWLTPAGRTLARIRPRGWWAPARPSLAGRSPP
                     RSQRTNRKHDASAIRRRME"
     gene            11027..>12773
                     /locus_tag="VFK00_12130"
     CDS             11027..>12773
                     /locus_tag="VFK00_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HET7593736.1"
                     /translation="MLRPRHLACAVALALASSAAFAQSAPPAQPSTHAGLFDQVVVFG
                     DSLSDDGNLSLAMELPQIMRFTTNPGQVGVEEIAGYFGAPVTPSLAGGTDFAFGGAGV
                     VNNSPGTPSTVPTLPSQLGLYLQATGGKADPNALYSVWGGANDIFYAGTSVVAAATAQ
                     TLIQQTIAAQVQQAITFGLIPNDPDAIAAFTAQITPAVTEQVTAAVLAGAGVSSLMTA
                     DQAQASIQQAAATEVGMIQQLGNSGARYVMVFNLPNIGLTPSGIAQGPDAAAQLTGLA
                     LTFNNTLNAGLANAGVNIIPVNAFAMLNEIIADPGRYGFTNVTEPACTGGDGSSFECL
                     PAGTPGATVTYQPGTENTYLFADGVHPTTAAHAILAQYAESIVIAPGQMSLLAEAPLQ
                     VNESLKRSIINQGTAGMAAAPGNGLRLWANYDYARQRLDAQVNSPKSSNDVNTLNIGA
                     DVHPSEMVTAGMALTIGQQKDDFAGNAGGFKLQDILASGYVMWNWANQGYVGAIGSFG
                     HLGYSNIHRNIPIGPTVRYEQGSTSGSHTAFALTGGWWIDFGSWRTGPYGDLSWQRIK
                     LDGFSENVTRDLINGG"
BASE COUNT         2150 a         4331 c         4137 g         2155 t
ORIGIN      
        1 gtgggcgtcg acgcaagcgg caacttcatt gccccggcgg tgctcaccgt gccgctgcaa
       61 ttgctcgcgt accacgtcgc ggtgctgcgc ggcaccgacg tcgaccagcc gcgtaacctc
      121 gcgaagtcgg tgaccgtgga gtagaagtgg ggcagcgcgc gcacttggca ccccgaagtg
      181 ttcgtcattc cggaatcgcg caccgacgtc gggactgcgg aggcacaccg gcctagccga
      241 tggatggcga gcagcggatg cggcgagttt ccggcgtctt ttttgacctc caacgtgccg
      301 aaaagctgat cctcgccttt acccgacgat taccgaaaga agagtcgacc gaacggcggg
      361 cttgcctcat tcgcatcggg atttcggggg gtcagggtgt cggtggaatt tacaatttgc
      421 gccatcccgc tccaatctga attatcgtaa tattttgttt gtaaatgctt ttctcctgcc
      481 tggcacgtta gatgctgcaa tcgcggcgcg aacctcgttc gcaagtcaag aaccgccggg
      541 aggcggttcg atccagacca ccaagcaggg gacttcaata tggagatttc caggatgctg
      601 caaatgcggc gcgcgatctt gacgacgggc gcactgttct tgatcggatg catcagcacg
      661 cccgcaatgg cgggtttcat ggcagccaat gacggatttt tcagttatac cgggacagtc
      721 acggcccccg acggccacac ttatacgatc ccggcttaca cctcgacgcc caacggcacc
      781 agctacgtgg gtcgcgacgc cagcgtgtac gtgacgagca acgctccgac cgtcgacacc
      841 ggcgccggct acgagaacag caccgttttc gagacgaact ggtactcggg caccagcaat
      901 ccgaacaaca ccgacacggg tttcgtccaa ctctccgacc tggcaagcca gggcgtgacc
      961 agcgtgagcg ggggttggac cgacaacagc tatacgacct ttactttcac cgtggtcggt
     1021 aatgcgggca gcggtgatcc cacctacgat cggctctggg ctgcaccaag agtgggcggc
     1081 cccgccactg atactgccgg agcgtttggc gggtactcgc tggtgctgac cgccacgttt
     1141 gccccgggtg cggtgaccga agagtcaccg ggctggtttt cgacggatgc cacgccgctg
     1201 tcgatcacgg gcagcttttc cggcctgttc accaataccg ggacgtatgc gcccggcctc
     1261 tatcaattcg acttcgactt cctcgatcag agctgggcag gaaacaatgg cgtgggcggc
     1321 ccgagttact tcggtgcctc ggcggttgcg gtgccggagc ccgcgaccct cgggctgttc
     1381 gggttcggat tgatggcgct gggcctgtgt gtgcggatgc gccgcaggtc gctcgcctga
     1441 caaaggcggc agcggcactc gatgtgagga acggcgcctt cgggcgccgt tcccgttgcg
     1501 gttcctcaac caggcccgaa cggcaactgc tcaagatcga cgttgccgcc ggtcagcacc
     1561 agcccgaccc gcttgcccgc gaatcgttcg cgttgcgcga gcacggccgc gagcacggtg
     1621 gcggaggaag gctcgacggt ctgtttcagt tctttccaga ggaggcgcat cgccgccaac
     1681 gtggcagcgt cgtcgacggt gacgatttcg acgtggtgct gttccagcat cgcgaggttg
     1741 gggatgccga tggtggcccg caggccgtcg cagatcgtct cgggcacgac gtcggtgacg
     1801 cgctggccgc gccccaggga ctggaatgca tcgtcggcgc cggcgggttc cgcaccaacg
     1861 acatcactcg acggctgcaa cgcgcgcacc acgatggcgc agccggaaac cagcccgccg
     1921 ccgccgaccg gggcaatcac cgcgtccaac tcgggtacct gttgcagcaa ctccagcacc
     1981 acggtgccct ggcccccgac cacgcgggca tcggcgtagg gatgcaccag cgtcgcgccg
     2041 gtttcccgct gcacccttgc gcaggtttct tcgcgggccg tttgcgtggg tgcgcagcga
     2101 tggatgatgc cgccggcgcc ctcgatgttg gcgagcttgg cagcgacagc accctcgggt
     2161 accacgacgt gcgcggggat gccgcgcgtt gcggcggcaa gcgcgagggc cgcgccgtgg
     2221 ttgccggagg aatgcgtgac cacgccacac cgcgcttccg catccggcag cgaccatacg
     2281 gcgttgcagg cgccgcggaa cttgaaggcg ccgccgcgtt gcaggtgctc gcacttgaag
     2341 aacatcgccg caccggccat cccgtcgagc gtctcgtcgc tcaacacggg cgtcacgcgc
     2401 gcgtgcgcgg cgatccgggc ggcggccgca cgaatgtcgg ccagcgtcgg ggcaatgaag
     2461 tcggccatgg tggcgcatcc tcgcacaccc agggcgttcg gcgcagaatg gaccacgtcc
     2521 gcgggcggca aggtccggcc ggaccttttg cgcgcatggc gcgccctacg tgcaatgcac
     2581 ggccgcgcat tggcgggctc ctgcagcaat gcgcgctccc tcgaggcatt cagggcaagg
     2641 gcgccgacgc cgctggtaaa atgaactgat ggatacacaa accgacgccg gcgcccccgc
     2701 cacggaaacg atcgtccgcc tcgccgatta ccgcccgcca gcctggcggg tcacacatgt
     2761 cgacctcgtg ttcgatctcg gcgtcgacga aaccatcgtg cgctcgaagc tgatcctggc
     2821 ccgcgaccgc gacgagccgt tgcatctcga cggcgaggac gtggaactca tcgaggcgcg
     2881 cgtggacggc cgcaagctcg gcgaaacgga atacgtcgtc gatcaaaatg gcttgaccat
     2941 cgcggacgtg cgcgacggca gcacgctgga actggacgtg cgcatccgtc cggcgcggaa
     3001 cagccagttg tcgggcctgt acctttcggg ttcgcgcgac cgcggcttcc tgctgacgca
     3061 gtgcgaggcg cagggattcc ggcgcatcac cttctttccg gatcgcccgg acgtgctggc
     3121 gcgctacgac gtcaccctgc gcgcgccgaa ggcgcggttt cccgtgttgc tggcgggcgg
     3181 cgagccgatg gagtccggcg ctctcgacga tggccgtcac ttcgcgcgct tcgtcgatcc
     3241 gcatccgaag cccagttacc ttttcgcgct ggtcgcggga tacctcgaaa agatcgaacg
     3301 gccttatcga accgccgatg gtcgcgatgt caccctgcgc ctgtgggccg aagacggtgc
     3361 gatcgatcgc tgccactacg cgatggatgc gctggagcgc gcgatgcgct gggacgagcg
     3421 cgcctacggc cgcaattacg acctgccggt gttcaacgtc gtcgcgaccg atgatttcaa
     3481 catgggcgcg atggagaaca agggcctcaa catcttcaac tcgaagtacc tgctggccga
     3541 cccgcgggtc accacggacg acgagtaccg ccgcgtcgaa gcggtgatcg ggcacgagta
     3601 tttccacaac tggagcggca accgggtcac ctgccgcgac tggttccagt tgagcctcaa
     3661 ggaaggcttc acggtgttcc gcgagcattc gttctgcgag gacatgcact cggccgcgct
     3721 gaaacgcatc gaggatgtcg cgacgctgcg ccgcgcccaa ttcgcagaag acgcggggct
     3781 gctcgcgcac ccggttcgtc ccgccgaata ccaggcgatc gacaatttct acaccgcgac
     3841 ggtgtacgag aagggtgccg agctgatccg gatgctcgcg ggcaaactcg gccgcgacgg
     3901 cttccggcgc ggcaccgact tgtatttctc gcgtttcgac ggccaggcgg tcacgatcga
     3961 ggacctgttg gcggcgctcg gcgaagccaa cgacgccgac ttgtccccgt acctggcgtg
     4021 gtatgcgcaa gcgggcacgc ccgaggtccg cgccagcggc gaatacgacg cgccggccag
     4081 gcgctacacc ctcacgctgt cgcagcgcac gccaccgacc cccggacaac cgcacaagca
     4141 accgctgccg attccggtgc ggatcgcatt gttcgcgcgc gcgggtgcac cgttggcatg
     4201 ccgcgtggat ggtggcaatc caaacgcttc aaccgaccac gtgctggaac tcgaccgtgc
     4261 cgaacagcgt ttcgtgttca cggacgtcgc ctcggaaccg acgccatcgc tgtgccgcgg
     4321 attctccgcc ccggttcgcg tcgcgttcga ctacagcgcg gaacaactcg cgttgctgct
     4381 cggccacgaa accgaaggct tcgatcgctg gaacgcggcg caggaattgg cgggccgcgc
     4441 cttcgacgag atactggccg gccagcgcga acatccggcg gtggacgcat ggctggacgc
     4501 attggcgcgg ctgttcgccg agcgcgcgcg ttcgatcgat cccgccctgc tcgcggactt
     4561 gctgtcgctg ccttcggcga tcgaactcgg cgtgcgccac gccccgttcg atccggacgc
     4621 cgtgcacgcg gcgcgcgagg cgctcgagaa tgcactggcg gcacggctcg gtgaagcgct
     4681 gcgcgcggcg taccgcgaac tgcacgcgaa cgaaaccggt tcgctcgacc aggatgcggc
     4741 cgcacgccgg cgcctgaagg cgcgggtgtt ggcgctgctt gcaaggttcg accctgaaca
     4801 gggtgccgaa ctcgcattcg cgcagtaccg cgatgcgcgt ggcatgaccg accggttggc
     4861 tgcgctggcg acgctggtgc gccgtggcgc accgcaagcc gccgatgcgc tgcgcctgta
     4921 ccgcgaaagc ttcgacggcg acgcgctgac actcgacaag tggttctcgc tgcaggccac
     4981 cgcgccggtc cacgacatgc tggcgcgcgt gcaggcgctc gaagccgatc cggccttcga
     5041 cctcgccaac cccaaccggg tgcatgccct gctcggcgcg ttcgcgcgcg ccaatcccgt
     5101 cgcattccat cgacccgatg gtgcaggcca tcgctggttt gcgggcaagc ttgccgagat
     5161 cgacaccagg aatccacagc tggccgcgcg tctcgccaag gcgttcgagg actggccgcg
     5221 gctggagccc ggccgccggg cttccgccga gggtgccttg cgggaactgc tggcgcgcgg
     5281 cggccactcg ccggacctca cagaaatcct gacccgcgtg ctggccgccg acgaccggaa
     5341 gccgggatcc ggcgcgtaga cgcgtagccg ggatggagtg aaacgggaaa ctcccgcaaa
     5401 ccgctccccg attccactgc gtttcatccg gggtacggag ctggccgagc ctcttcgaaa
     5461 cgtggacctg gcgcgtagcc cggattgaaa cgactgcgtc tgcaggatcg caaacgcagt
     5521 cggcgcaacc ggcccgaaag gcgagtgcca aggatggccg agtcgcgcgg gcgagacatg
     5581 gatgtcgcga atcaatcagg gaaagtctcg cgaagcaccc ggatgacact tcgatccatc
     5641 cgggctacga gccggctgct gaatgtcggt actccgtgcc tgcacgcggc cgtccgaagg
     5701 tgtaccatcg ggtacaggaa ttggcggcag gcccggaggt gtgccatgcg caaaactggt
     5761 gaaatgcggg cagtggcgcg cggtgcgcgt ctggcggtgt tcggggccgg ggtcctgctg
     5821 ctggccggct gcgctaccgg ctattcattc gtgcaaccgg gcgttgcggg cagcggcggc
     5881 tattacaccg gcgacggccc ctactcaggc caaggttatt acgactacta cggaacagga
     5941 ccttactacg ctggcaccag cgggtatggc tactacaacg gcacccggcc ttacagcagt
     6001 ccctacgggt actacgggcc aggatatggc tacgggtacg gttacgacta cggttcgcag
     6061 tggatattca acctcgggat ttccaacgtc tggaatttcc cgggctactg ggggccgtgg
     6121 tacacgaccg gttacgcctc atggggatgc ggttcgtggc gctgcgggaa tcgccaccac
     6181 gggcatcacc acgatcacga caacggatcc tggcggcatg acgatgcctc ctcgcttccg
     6241 gtcgatgttc cccacaccgc ggggaattcg cagccggact tcaacgccgg ccgcgagcga
     6301 gccatggaga ggactgcttg gcggcacgac cactacgtgc gctcgccgcg gcaaggcgcg
     6361 gcggtgccga acttcagcgc cgttccggcg atgcccagct acagtggcgg ccaagcgccc
     6421 cgtgcgacat ccttcggcac ccctgctgcg cgtcccgcgg ctgctcccat gaggatgccg
     6481 gcgccgcagg ctgccagccg cgcaccggtt gccgcgccgc aaccgagttt ccggcctgcc
     6541 atgccgatgc gggccgctcc gccggccgct tcgcgtacag gtaccaacac cggcacgaag
     6601 gtccagtagg aggctcggtc gaccgctgcg ccaggcaaaa aagaaggcgg ggagcaaggt
     6661 gtgtcacgct ccccgcctcc gccccccgaa tcgattcagt gcgagacgcc gaagaccatg
     6721 tcgtccagct cgcacagccg ccgcccgacc ggatggttcg ccgcctccgg cgaggccccg
     6781 gcggggcggc attcgcggat cccctcggcc tcgagctcga gtcgtcccat ctggaccagc
     6841 tcgtcgtggt gccggcgcac ctcttcccat tcttctttgg tcatgtcgat gaagttcatt
     6901 gacagctctc ctgttcttgc gggaaacccc ggaaaccccc tccggatcct tccgaatcct
     6961 tcaagtggcg ttcatccgca tccgctgata ctcttccgcc aaaggggcag tcgcgatatc
     7021 gtttcgccac ttcggcaccc gatgattttt cccaatctgg cgcccggaac acggttactc
     7081 tggattcccc ctgttcctga tgaccgccgg gcgccagttc ctttcgtgca tcgcctttcc
     7141 tggtgcttcc gccgacgcgg agaccgggtt gttgctgccg gtctcctgcc gttcgcagcg
     7201 gctgcgtcgg cggagcttgg gagtatttct gcaggtcgca tgccaatcca tcgggcacgg
     7261 cagcggaaat ttgcaagaga atgtgacgtc gccgggtagg ggccggatta cgtgcaccgc
     7321 acacggggcg cgtgacaatt cgcaacgatt gtctcgacgg gctttcggga ttcgataccg
     7381 atgcaggcgg cgtgatcgtt gggttcgggg accaacgttc aaggcaagtg acacgcggcg
     7441 accgcttctg acctgatcgt ggtctgtccg gaagcgtagg gcagtcaccg gtcgccggcg
     7501 cgaggaatgg cgacggatag tgtccgcccc acacgatagc ccagccgcgc gacgctcaag
     7561 cacataccta tggattccat gcaccgttat gacgttatcg tggtcggcgg cggccatgcc
     7621 ggcaccgagg ccgcgctggc gtcggcgcgc tgcggcgccc gcacgctcct gttgacccac
     7681 aacatcgaaa ccatcggcca gatgagttgc aatcccgcga tcggcgggat cggcaagggc
     7741 cacctcgtca aggagatcga tgccctcggc ggcgcgatgg cgcacgcggc cgatcgtgcc
     7801 gggatccagt ggcgcacgct caatgcatcg aaaggacccg cggtgcgtgc gacgcgctgc
     7861 caggccgacc gcgtcctgta cagggcggcg attcggcaca ccgtcgagcg gcagccgaac
     7921 ctggagctgt tccagcaggc cgtcgacgac ctcgtgatcg aatccgggcg cgtcgtcggc
     7981 gtcgtcacgc aaatgggttt gacgatccgt gcccggcgcg tggtgctcac cgtcggcacc
     8041 ttcctcgccg gcaagatcca catcggcctg gaaaaccact cgggcggtcg cgcgggcgat
     8101 ccgcctgcaa cgacgctcgc gaagcgtctg cgcgaactgc cgctcggcgc cgatcggctg
     8161 aagaccggca cgccgccacg catcgatgcg cgcacgctcg attattcggt gttcgaggaa
     8221 cagcccggcg acgatccgtt gccggtgttc tcgtacctcg gctcgcgcga cgagcatcca
     8281 cgccaggtgc catgctggat cacccatacc agcgagcgca cccacgacat catccgcgcg
     8341 ggcctcgacc gttcaccgct ctataccggc aagatcgaag gcactggtcc acgttattgc
     8401 ccgtcgatcg aggacaaggt ggtgcgattc gccgacaagg cctcgcatca ggtcttcatc
     8461 gagccggaag ggctggacgc catcgaaatc tacccgaatg gcatctcgac ctcattgccg
     8521 ttcgacgtgc aaacggaact ggtgcattcg atccccggct tcgaacgcgc gcacatcacg
     8581 cgccccggct acgcgatcga atacgactat ttcgatccgc gcggactgca cccgtggctc
     8641 gagaccaaag ccatcggggg tttgcatctc gcgggccaga tcaacggcac cacgggctac
     8701 gaggaagccg cggcgcaagg ccttgtcgcc ggtctgaatg ccgcgcgcat ggctcgcggc
     8761 ggggaaccct ggtggccgcg ccgcgacgaa gcctacatcg gcgtgctgat cgacgacctc
     8821 accaccaacg gcaccatcga gccgtaccgg atgttcacgt cccgcgccga atatcgcttg
     8881 cacctgcgcg aagacaacgc cgacctgcgc ctgaccgaga caggtttcca actcggcgcg
     8941 gtgccgcggg aacgtttcga cgcaatgcgc gcgaaacgcg atgcggtgga acgcgaaacc
     9001 gcgcgtttga agtcgctatg tgcaacgcct gccaatgcgc tcggcgcggc gttgcagtcg
     9061 gaacggggca tcacgctttc gcacgaagcc accgcgctgg agttgctgcg ccgcccggaa
     9121 ctcggctact cgatgctcgt cgcgacgaac ggtttcggtc ccgcggtcga gcgcgaggac
     9181 gtcgccgcgc aggtcgaagt gcaggcgaaa tacgccggtt acctggagcg ccagcgcgac
     9241 gaaatcgagc gccagcgcca ccacgaatcc acgtcgattc cggatgactt cgattacgac
     9301 cgcgtgcgcg gactgtctgc cgaagtgtcg gtgaaattga agcgtgtgcg tcccgcgacc
     9361 gtcgggcagg cgcgccggat cagcggcgtg acgcccgcgg cgatttcgct gctgctggtg
     9421 cacttgaagc ggggagacag ccgcgcggcg tgacgtcacg gcataggccg gctcatgccc
     9481 aaaggacgga gccggcgatg gcgaaccggt gcgccagatc cgctgcgccg gctccatcgc
     9541 gtcgctcctt cagccggcct atttgaactg aacgcccgcg ctacgcgccg accgcgttcg
     9601 gacctcgtta acgcgcccgc cgcaatcatg gtccaagcgt gcaatgcgcg cacggcgcgt
     9661 aacgcgtcaa tccatttcat gggctaccgg ccgcaggcac ggctgcggcc atacggagga
     9721 acaccgcgcg gatgatccgc gaagccaccg acaactggac cgagaccgtc tatcgcccat
     9781 tgcgtcgcgt aggccgacgc ctctcgcacg aattcgcgac cggcgcgccg cgggccagtc
     9841 ggcggcctga acgctacctg gcgctcgaca atgcccgctt cgtgctcatc gcgctggtgg
     9901 tggtcggcca cctgctggaa caactcgtca acacggggcc gttttcggac acgctgtacc
     9961 gctggatcta cctgttccac atgccgggct tcatcctgat ctccggcgcg ctgtccaagc
    10021 ggacactgac gcaccggcgg ttgctcgggc tcgcgaccgg gctgctggtg ccctacgtca
    10081 tcttccagac gttgtacccg gcctgggacg ccctgctctt ccccggcaat gactggtcgc
    10141 agggttacct cacgccgtac tggctgttgt ggtacctgcc gagcctcgtg tgctggcggc
    10201 tgatgctgcc gttgttcgcg catcgctggg cactaccggc ggcggtcgcg atcgcactcg
    10261 ccgcgggcct cgcgccgtgg atcgggtatc cgttctcgct gtcccgcacg ctggtgttct
    10321 ttccgttgtt cctgctcggc caccggatcg gggcgcagca gttgcagcgc ctcggcgctt
    10381 cgcgcggcgc aagggtttgc gcggtggtgg tcctcgcggc cgcgggcgcc ggcgcgtggt
    10441 ggctgcgcgg gctggatccg caatggctct acgcatcggt cggctacgcg acacttaatg
    10501 tggatgccgt gccgggcgcc gcgatccgac tggccctgct ggcggcgtcc tcggcgtgcg
    10561 cattggcggc catcgccctg gtgccccgcc ggcccctgtg ggccgggttc ggtcggcgca
    10621 gcctcaccac ttatttgatg cacggcttcc tgatccgcgg cttgcttgct gccggcgttt
    10681 tcgcatggct ctcgcacgag atcgtgccgc cgctgcaggt gctcctgtgc ctggcgtgcg
    10741 gcgcgctgat cgctgcactc ttctccacgc ggttcatgga caagctggcc acgccgttga
    10801 cgcgtccggt gcgatggctc acgcccgcag gccgcacctt ggccagaatt cgtccccgag
    10861 ggtggtgggc accggcccgg ccttctctgg ccgggcgttc accgccgcgc agccagcgga
    10921 caaaccgcaa gcacgacgct tccgccatac gccgccggat ggaataaaac aaacgtctgt
    10981 attaggcttg cggcggtccg ccactcggcg ttgggagatt ccccgaatgc ttcgcccccg
    11041 tcacctagcc tgcgccgtgg cgctggcgct tgcaagttcc gccgccttcg cacaatccgc
    11101 gccccccgcg cagccttcca cgcacgccgg cctgttcgac caggtcgtgg tgttcggtga
    11161 cagtctgagc gacgacggca acctgtcgct cgcgatggaa ctgccgcaga tcatgcggtt
    11221 caccaccaat ccggggcagg tcggtgtgga ggagatcgcc ggctacttcg gggcgccggt
    11281 gacgccttca ctggcgggcg gcaccgactt cgcgttcggc ggcgccggcg tggtcaacaa
    11341 ctcgcccggc acgccctcga cggtcccgac gctgccgtcc cagctcgggc tgtacctgca
    11401 ggcgaccggc ggcaaggcgg atccgaacgc actttattcg gtctggggcg gcgcgaacga
    11461 catattctat gccggcacct cggtggtggc cgccgcaacc gcgcagacat tgatccagca
    11521 gaccatcgcg gcgcaggtcc agcaggcgat cacgttcggc ctgatcccga acgatcccga
    11581 cgccatcgcc gcgttcacgg cccagatcac gccggccgtc accgaacagg tcacggcggc
    11641 ggtactcgcc ggggccggcg tatccagcct gatgaccgcg gaccaggccc aggccagcat
    11701 ccagcaggcg gccgcgaccg aggtgggcat gatccagcag ctcgggaact ccggcgcgcg
    11761 ctacgtcatg gtattcaacc tgccgaatat cgggctgacg ccgtcgggca ttgcccaggg
    11821 cccggacgcc gcggcccagc tcacagggct ggcactgacc ttcaacaaca ccttgaacgc
    11881 ggggctcgcc aacgccggcg tcaacatcat cccggtcaac gccttcgcga tgctcaatga
    11941 aatcatcgcc gacccaggcc gctacggatt caccaatgtc accgagccgg cctgcaccgg
    12001 tggcgacggc agctcgttcg aatgcctgcc cgccggaacg cccggcgcca ccgtcaccta
    12061 ccagccggga accgagaaca cgtacctgtt cgccgacggc gtacatccga ccacggccgc
    12121 ccacgcgata ttggcgcagt acgcggaatc gatcgtcatc gcgcccggcc agatgtcgct
    12181 gctcgccgaa gctccgcttc aggtcaacga gagcctcaag cgctcgatca tcaaccaggg
    12241 cacggccggt atggccgcgg cacccggcaa cggcttgcga ctttgggcca attacgatta
    12301 cgcgcgccag cggctcgatg cacaggtcaa ttcgcccaag agcagcaacg acgtcaacac
    12361 cctcaacatc ggcgcggacg ttcatccgtc ggaaatggtg accgccggga tggccttgac
    12421 catcggccag caaaaggatg acttcgccgg caacgcaggc ggattcaagc tccaggacat
    12481 cctggccagc ggctacgtga tgtggaactg ggccaaccag ggatacgtcg gcgcaattgg
    12541 aagcttcgga catctgggct acagcaacat ccaccggaat atcccgatcg gcccgaccgt
    12601 ccgctacgag cagggcagca cctccggttc gcacacggcc ttcgcgttga ccggcggctg
    12661 gtggatcgat ttcggcagct ggcgcacggg cccttatggc gacctttcct ggcagcgcat
    12721 caaactcgac ggcttcagcg agaacgtgac gcgcgacttg ataaatggag gcg
//
LOCUS       DASSNH010000321         6751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_183342, whole genome shotgun sequence.
ACCESSION   DASSNH010000321 DASSNH010000000
VERSION     DASSNH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183342"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFK00_12135"
     CDS             complement(<1..948)
                     /locus_tag="VFK00_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7593737.1"
                     /translation="MLDKVLIANRGEIALRVLRACQTMGIKTVAVHSTVDRNLKHVGM
                     ADEAVCIGPAPSAQSYLNVPAIIAAAEVTDASAIHPGYGFLSENADFAEQVERSGFVF
                     VGPTAPVIRMMGDKVEAIKAMKAAGVPCVPGSDGPLDDDVERSIRIARDIGYPVLIKA
                     AGGGGGRGMRDVHTEAHLGNAIATTRAEARAAFGNDQVYMEKFLENPRHVEIQVLADG
                     QGNAIHLCERDCSMQRRHQKVVEEAPAPGITPEMRAEIGRICTDACIRIGYRGAGTFE
                     FLFQDGRFYFIEMNTRIQVEHPVTELITGVDLVREQLMIA"
     gene            complement(954..1424)
                     /gene="accB"
                     /locus_tag="VFK00_12140"
     CDS             complement(954..1424)
                     /gene="accB"
                     /locus_tag="VFK00_12140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7593738.1"
                     /translation="MDLRKVRKLIELLEESNLSELEIREGEETVRLSRFPATGAIAQQ
                     FAPAPQPMMAPVVAASAAAAPSSSAASPQESGLPAGNVVRSPMVGTFYAAPSPGAEPF
                     VTVGQQVKVGDPLGVIEAMKMFNQIEAEVAGTVIAIVAENGEAVEFDQPLFVIG"
     gene            complement(1436..1921)
                     /gene="aroQ"
                     /locus_tag="VFK00_12145"
     CDS             complement(1436..1921)
                     /gene="aroQ"
                     /locus_tag="VFK00_12145"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7593739.1"
                     /translation="MAQILVIHGPNLNLLGGREPAVYGRHTLAEIEAMLVDRAEQAGH
                     GLQTFQSNAEHALIERVQRAPNEDIGFILINPAAFTHTSVALRDALAAAAVPFIEVHL
                     SNPHAREPFRHRSYFSDLAVGVVAGFGADSYRYALDAAIQRLGAHPPIHPDPSAPAEG
                     H"
     gene            complement(2084..2662)
                     /locus_tag="VFK00_12150"
     CDS             complement(2084..2662)
                     /locus_tag="VFK00_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET7593740.1"
                     /translation="MVLILVLACVAAAGGWWLQARLTAAPRQQQPAAPAGVTVLGPGD
                     SVGAYALPGIDGRTTSLAAWRGKVVLLNFWATWCAPCREEMPALAKLQREHAADGLQV
                     IGIAMDQPQSAAAFLKRMPVDYPILIGIDADPVPTTTFGDTAGLLPYSVLIGRDGRIL
                     ETKLGVLDPPTIDAWLETASEGADGRSAPKGA"
     gene            complement(2674..4506)
                     /locus_tag="VFK00_12155"
     CDS             complement(2674..4506)
                     /locus_tag="VFK00_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HET7593741.1"
                     /translation="MPAPRKSLSRLLLVAALLLAAAGAYAQSTSPGGGLLPVTEAFAL
                     KATVDRPGRIALHFDIAKDYYLYRGRIHAKILTAGVKAGALDLPPGKQEHDEYLGDVE
                     IYHGSVDGTLPFSASGAMPATLKVTVSYQGCHEVTPKICYPPNAETFTLPTSGGGPVG
                     GSRAAGGPAGTAITASLGTSPPSSPQPPGAGGRSGTTSAAGPLSIGLPFALLLAFAGG
                     LILNLMPCVLPVLAIKAVGVLESNTTRAESRRHALAYGAGVIATFLAIGLAILGLRGA
                     GQATGWGTQLQQPVIVAVLACVLFAVGLSMSGVVSFGSRLGNLGGGLTRRGGTAGHFF
                     TGVLAVIVASPCTAPFMGVALAYAFVAPPAHQILVMLVLGIGLALPLTLIGLVPMLAR
                     LLPKPGRWMETLKQVLAFPMYLSAVWLVWVLAHQRGADAVALVLVAMVLVAAAAWWHG
                     RAPSGRRLGHVFTAVLVLAAIASFFAVARVAPPTRSADARGDAVVFSPGKLQSLRNAG
                     TPVFVDIGADWCVTCKANEYAVLDTSSFQDLLETTGAVYMKGDWTDVDPEIGAYLREF
                     HSPGVPLYVVYPRGGGAGHALPTVLTASLVRHALVRADTEPAAR"
     gene            complement(4812..5381)
                     /locus_tag="VFK00_12160"
     CDS             complement(4812..5381)
                     /locus_tag="VFK00_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593742.1"
                     /translation="MNSTMLFGRRSSFALALLAAVALPPAASEDAPTDAQVIASARSA
                     APMEVAKDATIMAMEAGGKMRTVRQGHNGFTCIPDSPSTPGPDPMCMDANGLAWARAW
                     AGHEPPPAGKIGFMYMLQGGTDASNTDPYATEPNSGNHWVKTGAHVMVVGADAAFYDQ
                     YPKSADPNTKMPYVMWAGTPYQHLMVPVQ"
     gene            complement(5582..5905)
                     /gene="cutA"
                     /locus_tag="VFK00_12165"
     CDS             complement(5582..5905)
                     /gene="cutA"
                     /locus_tag="VFK00_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET7593743.1"
                     /translation="MATPLLCLSTCPDAETATRIARALVEERLAACVNRLPGVQSVYR
                     WQDKVVDDAEVLLVVKTTSERFEALRDRLVELHPYEVPELVAIEVSGGHRAYLDWIAA
                     ETVPQ"
     gene            6108..6398
                     /gene="groES"
                     /locus_tag="VFK00_12170"
     CDS             6108..6398
                     /gene="groES"
                     /locus_tag="VFK00_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009684394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7593744.1"
                     /translation="MKLRPLHDRVIIKRLEAETKSAGGIVIPDTATEKPIKGEVVAVG
                     TGKIQEDGKVRPMGVKAGDKVLFGKYSGTEVKVEGEELLVMREEDLMAIIEG"
     gene            6524..>6751
                     /locus_tag="VFK00_12175"
     CDS             6524..>6751
                     /locus_tag="VFK00_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET7593745.1"
                     /translation="MAAKEIRFSEDARSRMVRGVNALANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEVELADKFENMGAQMVKE"
BASE COUNT         1117 a         2222 c         2337 g         1075 t
ORIGIN      
        1 cgcgatcatc aactgttcgc gcaccaggtc gacgccggtg atgagttcgg tgaccgggtg
       61 ctcgacctgg atccgggtgt tcatctcgat gaagtagaag cgcccgtcct ggaacaggaa
      121 ctcgaaggtt ccggcgccgc ggtagccgat gcggatgcag gcgtcggtgc agatcctgcc
      181 gatctccgcg cgcatctccg gggtgatgcc gggtgccggt gcttcctcga ccaccttctg
      241 gtggcggcgc tgcatcgagc agtcgcgctc gcacaaatgg atggcgttgc cctggccgtc
      301 ggcgagcacc tggatttcga cgtggcgcgg gttctccagg aacttctcca tgtatacctg
      361 atcgttgccg aacgcggcgc gcgcctcggc gcgggtggtc gcgatcgcat tgccgagatg
      421 ggcctcggta tgcacgtcgc gcatgccgcg cccgccgccg ccgcccgcgg ccttgatcag
      481 caccggatag ccgatgtcac gcgcgatccg gatgctgcgc tcgacatcgt cgtcgagggg
      541 gccgtccgag cccggcacgc agggcacacc cgcggccttc atcgccttga tggcttccac
      601 cttgtcgccc atcatgcgga tcactggcgc ggtggggccc acgaacacga agcccgatcg
      661 ctccacctgc tcggcgaagt cggcgttttc ggagagaaac ccgtagcccg ggtggatcgc
      721 ggatgcgtcg gtgacttcgg ccgcggcgat gatggccggc acgttgaggt aactctgcgc
      781 ggacggtgcc ggcccgatgc atacggcctc gtcggccatg ccgacgtgct tgaggttgcg
      841 gtcgacggtc gaatgcaccg cgacggtctt gatgcccatg gtctggcagg cgcgcagcac
      901 gcgcagcgcg atctcgccgc ggttcgcgat caaaactttg tctagcacgg gcatcagccg
      961 atcacgaaca gcggttggtc gaactccact gcctcgccgt tttcggcgac gatggcgatg
     1021 acggtgccgg cgacttcggc ctcgatctgg ttgaacatct tcatggcctc gatcacgccc
     1081 agcgggtcgc cgaccttcac ttgctgaccg acggtgacga acggttccgc gcccggcgag
     1141 ggtgcggcgt agaacgtgcc gaccatcggt gagcgcacca cgttgccggc gggcaggccc
     1201 gattcctgcg gcgacgccgc gctggaggaa ggcgcggcag ccgcggaagc agccacgacg
     1261 ggtgccatca tcggctgcgg tgctggcgcg aattgttgcg caatggcgcc ggtggccgga
     1321 aagcgcgaaa gccggaccgt ttcctcgcct tcccggatct cgagttccga gagattggat
     1381 tcttccagca gttcgatcag ctttcttact ttgcgcaggt ccatggtggc ctcggtcagt
     1441 gtccttcagc cggcgcggaa ggatcgggat ggatgggtgg atgggcaccg agtcgctgga
     1501 tcgcggcgtc cagcgcgtag cggtagctgt cggcgccgaa cccggcgacc acgcccaccg
     1561 cgaggtcgga aaaatacgac cggtggcgaa acggttcgcg cgcgtgcggg ttggacaggt
     1621 gcacctcgat gaacggaacc gcggcggcgg cgagcgcgtc gcggagggcc accgaggtat
     1681 gggtgaaggc ggccgggttg atcaggatga acccgatgtc ttcattgggc gcgcgctgga
     1741 cccgctcgat gagtgcgtgc tcggcgttgg actggaaagt ctgcagcccg tggccggcct
     1801 gctccgcccg atcgacgagc atggcctcga tctcggcgag ggtgtggcgg ccgtacaccg
     1861 ccggttcgcg gccgccgagc aggttgaggt tgggtccgtg gatgaccagg atctgcgcca
     1921 cgctcggcac gcccgcggag gttgaccgga agcccgcaag tctgaaccgt cattcatgct
     1981 ttgtccagcg ttcgggctgg aatcgcggga gatcggcgga gttccttgcg tgtttcatgc
     2041 tgcgatcgat ccatgattcg gccgcgacgc ggtccaagtg ggatcaggcc cccttgggtg
     2101 cggaacggcc atcagcgccc tcgcttgccg tttccagcca tgcatcgatg gtagggggat
     2161 cgagcacgcc cagcttggtc tcgaggatgc gcccgtcgcg tccgatcagc acgctgtaag
     2221 gcaagagccc ggcggtgtcg ccgaaggtgg tggtcggcac cggatcggcg tcgatgccga
     2281 tcaggatcgg gtagtccacc ggcatccgct tcaggaaggc agccgccgac tggggctggt
     2341 ccatcgcgat gccgatgacc tgcaggccgt ctgcggcgtg ctcgcgctgc agtttcgcga
     2401 gcgcgggcat ctcttcgcgg cacggtgcgc accaggtggc ccagaagttg agcagcacca
     2461 ccttgccgcg ccacgcggcg agcgaagtcg tgcgcccgtc gatgccgggc agcgcatagg
     2521 cgcccaccga atcgccggga ccgagcacgg tcacgccggc cggcgcggcg ggctgctgct
     2581 ggcgaggcgc cgccgtcaag cgtgcctgca gccaccatcc gcctgcggcc gcgacgcacg
     2641 ccaggaccag gatcaggacc atgcggcggt ccatcatcgg gccgccggct ccgtgtcggc
     2701 acggaccagc gcatggcgaa ccagcgaagc cgtcagcacc gtcggcagcg catgtccggc
     2761 gccgccgccg cgcggatata ccacgtacag cggcacgccc ggcgagtgga attcgcgaag
     2821 gtaggcgccg atctccggat ccacatccgt ccagtcgcct ttcatgtaca ccgcgcctgt
     2881 cgtctcgagg agatcctgga aactgctggt gtccaggacc gcgtactcat tcgccttgca
     2941 ggtgacacac cagtccgcgc cgatatccac gaacaccggt gttccggcat tgcgcaatga
     3001 ctggagcttg ccggggctga aaaccaccgc atcgccccgc gcgtctgcgg accgcgtggg
     3061 cggagcgacg cgcgcgacgg cgaagaacga tgcgatggcg gcgagcacca ggactgcggt
     3121 gaagacatgg ccgagcctgc gcccggatgg agcgcgtcca tgccaccagg ccgccgcggc
     3181 gaccagcacc atcgctacca atacgagtgc caccgcgtcg gcgccgcgct ggtgcgccag
     3241 cacccagacc agccacacgg ccgacaggta catcggaaac gcgaggactt gtttgagcgt
     3301 ctccatccag cgcccgggct tcggcagcag ccgcgccagc atcgggacca gcccgatcag
     3361 ggtcagcggc agcgcgaggc cgatgccgag taccagcatc accaggatct ggtgcgcagg
     3421 tggcgccacg aacgcgtagg cgagggccac gcccatgaac ggcgcggtgc aggggcttgc
     3481 cacgatcacc gccagcacgc cggtgaagaa gtgcccggcg gtgccgccgc gccgggtcaa
     3541 tccgccgccg aggttgccga gccgcgaacc gaaactcacg acgcccgaca tcgacagccc
     3601 caccgcgaac agcacgcagg ccagcaccgc gacgatcacc ggttgctgca actgggtgcc
     3661 ccagccggtc gcctgaccag caccgcgcag gcccaggatc gcgagaccga tggcgaggaa
     3721 tgtcgcgatc acgcctgcgc cgtaggccag ggcatggcgg cgcgattccg cccgagtggt
     3781 gttgctctcc agcacgccca cggccttgat cgcgagcacc ggcagcacgc acggcatcag
     3841 gttcaggatc aggccaccgg cgaaggcaag cagcagcgcg aaggggagtc cgatggaaag
     3901 cgggcccgcc gccgacgtgg ttccactccg gccgcctgcg ccgggcggtt ggggactgga
     3961 cggcggcgag gtaccgagcg atgcggtaat cgccgttccc gcgggacctc ccgcggcccg
     4021 gcttccgccg accggcccgc cgccgctggt gggcaacgtg aaggtttccg cgttcggcgg
     4081 gtagcagatc ttgggcgtga cttcgtggca gccctgatag gacacggtga ccttcagggt
     4141 ggccggcatc gcgccgctcg cgctgaaggg cagggtgccg tcgacgctgc cgtggtagat
     4201 ctccacgtcc ccgaggtact cgtcgtgctc ctgcttgccg ggcggcaggt cgagcgcgcc
     4261 ggccttgacg ccggcggtca ggatcttcgc gtggatgcgg ccgcggtaga ggtagtagtc
     4321 cttcgcgatg tcgaagtgca gggcgatccg gccgggtcgg tcgaccgtgg ccttgagcgc
     4381 gaaggcctcg gtcaccggca gcaggccgcc gcccggactc gtgctctggg cgtacgcgcc
     4441 cgcagcggcg agcaacagcg ccgccaccag gagcaggcgc gagagtgatt ttcggggagc
     4501 tggcatcttc atgactgcaa ggaaagaccg gcgcctgcca tcatcgttcc ggaatgcatt
     4561 tcggcgtacg acgtgacggt agtgcgcgaa cgcgaaccgc gggttgaact gcatcgcgca
     4621 gcgatgcagg ggcgaacgag ccggggagcc cgcaggaatt gtcgcaggca cggcgcgaag
     4681 ccgccagtcg acgcgtcgtc tggcggatgc gttcgactca gcgcccgatc cggatcgggc
     4741 gtgccggaca ccgatgcacc gcggcgggcg gcatggcacc gcccgggaat ccggctgcat
     4801 cggcggtttc gttattgcac cggcaccatc aggtgctggt agggcgtgcc ggcccacatc
     4861 acatacggca ttttcgtatt cgggtcggcg ctcttcgggt attggtcgta gaaggccgca
     4921 tccgccccga cgaccattac gtgcgcgccg gtcttcaccc agtggttgcc ggagttcggc
     4981 tcggttgcgt aagggtccgt gttgctggcg tcggtgccgc cctgcagcat gtacatgaag
     5041 ccgatctttc cggccggcgg cggttcgtgc ccggcccagg cgcgcgccca cgccaatccg
     5101 ttggcatcca tgcacatcgg gtccggcccc ggggtggacg gggaatcggg tatgcaggtg
     5161 aaaccgttat ggccttggcg caccgtgcgc atcttgccgc cggcctccat cgccatgatg
     5221 gttgcgtcct ttgcgacctc catgggcgca gcgcttcggg cgctggcgat cacttgcgcg
     5281 tcggtcggcg cgtcctcgct ggctgccggc gggagcgcga cggcggccag cagtgccagt
     5341 gcgaatgagg aacgacgccc gaacaacatg gtgctgttca tacgaacctc cagagggatt
     5401 gccccctgtc tgtccgcggc tggcgccgcc gcctgcaccg ctggcaggcc acttatgcta
     5461 ctccgaatgc ccggggcggg gcagccggta tcccttgtgg ggtagccgct ccgcaaagcc
     5521 cggcggcgga gccgactgca ccctcgaacg cgtccggttc aggatggcgt gtacaggcgc
     5581 ctcactgggg gacggtctcg gctgcgatcc agtcgaggta cgcccggtga ccgccgctga
     5641 cctcgatcgc gaccagctcg ggcacctcgt aggggtgcag ttccacgagg cggtcacgca
     5701 aggcctcgaa acgctcgctg gtggtcttga ccaccagcag cacctcggca tcgtcgacca
     5761 ccttgtcctg ccagcgatag acggactgga cgcccggcag ccggttcacg caggcggcca
     5821 gccgctcctc gaccagcgcg cgggcgatac gggtcgcggt ttcggcgtcc ggacaggtgg
     5881 aaaggcaaag cagcggcgtg gccatgggct cggaaatcgg tcggtcacaa ggcatggatt
     5941 gtaggagcgg gcggtgcccg cgaccgtctg gtcccactcg attcggtcgt gcgcatggcg
     6001 cgctcctact tgaaacggcc ggatccggac ctacaattgg cactcgtcgt tgttgagtgc
     6061 taacaacgcc aacccctttc atccccccca acgcagctgg agtcgttatg aagcttcgtc
     6121 cgttgcacga ccgcgtcatc atcaagcgcc ttgaagcgga aaccaagtcc gcgggcggca
     6181 tcgtcatccc cgacaccgcg accgaaaagc ccatcaaggg cgaagtggtc gccgtgggca
     6241 ccggcaagat ccaggaagac ggcaaggtcc gtccgatggg cgtgaaggcc ggcgacaagg
     6301 tgctgttcgg gaagtattcc ggcaccgagg tcaaggtcga aggcgaggaa ctgctcgtca
     6361 tgcgcgaaga agacctgatg gcgatcatcg aaggctgatc gagccctccg aattcccagc
     6421 cgtcattccg gcgcaggccg gaacccagct tcttcgcaag ctccctggat gccggccttc
     6481 gccggcatga cgatcgaacg caaagcatta caggaatcca accatggcag ccaaagaaat
     6541 ccgtttctcc gaagacgcgc gcagccgcat ggtgcgcggc gtcaacgccc tggccaacgc
     6601 ggtcaaggcg accctcggcc ccaagggccg caacgtcgtg ctcgagaaga gcttcggcgc
     6661 ccccaccatc accaaggacg gcgtgtccgt ggcgaaggaa gtggagctcg ccgacaagtt
     6721 cgagaacatg ggcgcgcaga tggtgaagga a
//
LOCUS       DASSNH010000322        21792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_1437532, whole genome shotgun sequence.
ACCESSION   DASSNH010000322 DASSNH010000000
VERSION     DASSNH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 21792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21792
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437532"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="clpX"
                     /locus_tag="VFK00_12180"
     CDS             <1..1198
                     /gene="clpX"
                     /locus_tag="VFK00_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7593746.1"
                     /translation="IAGPSVFICDECVELCNDIIREELEEKAASGRTQLPKPREIMET
                     LDQYVIGQTRAKKSLAVAVYNHYKRMESRAKNDDIELAKSNILLIGPTGSGKTLLAET
                     LARMLNVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQTGIVYIDEIDKI
                     SRKSENPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKHPQQEFLQVDTRNILFMC
                     GGAFSGLEKIIQQRSETTGIGFAAEIRSKERRQNVGTLLANVEPEDLVKYGLIPEFVG
                     RLPVVATLDELDEEALVKILTEPKNAITKQFKKMFDMENVEMEFRPDALTAIAKRAIK
                     RKTGARGLRTILEGVLLDTMYELPSLEHVSKVVVDEAVINGQAEPYLIYRGNLQPQAR
                     AEGDAA"
     gene            complement(1287..1718)
                     /locus_tag="VFK00_12185"
     CDS             complement(1287..1718)
                     /locus_tag="VFK00_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HET7593747.1"
                     /translation="MNDISIQEAQQEIVDEFGFFDDWTGRYQYLIDLGRKLPPLTDTE
                     MSPENKVDGCQSQVWIVVSGDAAQLDLRAASDSAIVSGLVALLLRVYSGRSAREILDT
                     DPGFINAIGLGKHLSPTRSNGLASMVKTIKARAERVLGEPG"
     gene            complement(1715..3097)
                     /gene="cysS"
                     /locus_tag="VFK00_12190"
     CDS             complement(1715..3097)
                     /gene="cysS"
                     /locus_tag="VFK00_12190"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7593748.1"
                     /translation="MSLRLYNSLTRRIEPFEPGDPARVTMYLCGPTVYSYVHIGNARG
                     PVVFDVLARLLRRKYPRVVYARNITDVDDKINAAAAEAGLPIAAIAEKFAAIYREDIA
                     KLGVAAPDVEPMATRHIAAIIAMCEALLASRHAYVAEGHVLFDVASFADYGALSGRST
                     DELIAGSRVEVAPYKRNPADFVLWKPSTPELPGWESPWGRGRPGWHIECSAMAAAHLG
                     ETIDIHAGGVDLVFPHHENEIAQSTCAHGGRVFARFWIHNGMLTFGGAKMSKSVGNVR
                     QLHALLEQYPPEAIRYALLSAHYRQPLDWSDALIEQSVATLDRLYGTLRDLASVEATA
                     ADADSPANLENALLDDLNTPEAMAELAKLADDARRAVSPAERSQARAALLAGGALLGL
                     LQQDPEAWFKRGAEQVDSTRVETLIEARRAARAARDFARADEIRNELAAMGIAIEDGP
                     QGTRWKVTTP"
     gene            3244..3780
                     /locus_tag="VFK00_12195"
     CDS             3244..3780
                     /locus_tag="VFK00_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7593749.1"
                     /translation="MTKILVSSLLLGFAVAANAQNYGPPPPPAAAQATNVRYGWAEVL
                     RVDPVYAAVAVPPQQECYPETVTREDNNHTGGTVLGAIVGGVLGNTVGKGDGRKAATV
                     AGAVAGGAIGNRVSAAHDRSYTSQQTVCRPVDRYAPQQQIVGYDVEYRYRGDVYMSRL
                     PYDPGERLRVRMTISPAD"
     gene            4216..5241
                     /locus_tag="VFK00_12200"
     CDS             4216..5241
                     /locus_tag="VFK00_12200"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HET7593750.1"
                     /translation="MTLRHFITTQDWSRGEIDALLARAAEFKRSPPAGALAGKSIALL
                     FFNPSMRTRTSFELGAFQLGGHAVVLSPGKDAWPIEFDVGAVMDGEAEEHVAEVARVL
                     SRYVDLIAVRAFPKFRDWATDREDRVIRAFAQYATVPVINMETITHPCQELAHALALK
                     EHLGELRGRKYVLTWTYHPKPLNTAVANSALLIATKMGMDVTLLCPTPEYVLDERYMV
                     FGEANARENGGSLRVTHDIEDAYRGADVVYAKSWGALPYFGRWDAEKPIRDAHEHFIV
                     DEAKMALTHRAVFSHCLPLRRNVKATDAVMDAPYCIAIDEAENRLHVQKAVMATLAAE
                     PEKETST"
     gene            5238..5720
                     /locus_tag="VFK00_12205"
     CDS             5238..5720
                     /locus_tag="VFK00_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7593751.1"
                     /translation="MTDHPHRYRVDVEWTGNHGSGTDGYRNYGREHVIRIEGKPEIAG
                     SSDPKFRGDGGKHNPEDMLVAALSTCHMLAYLHVATLAGVVVTAYTDTAEGTMVTVAD
                     GGHFTEVVLHPVVTITAASDASKAEAAHEDAHHACFIAASVNFPVRCEPSTVVEQGPD
                     "
     gene            5771..7057
                     /locus_tag="VFK00_12210"
     CDS             5771..7057
                     /locus_tag="VFK00_12210"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7593752.1"
                     /translation="MTKTSKTARPRFPVPGPEGKHIVLAFSGGLDTSFCVPYLAERGW
                     AVHTVFADTGGVGAAEHAYVEARAKELGAASHVTVDGGPALWDHFVKPFIWAGEGYQG
                     QYPLLVSDRYLIVEAAITRAMELGTRAIAHGCTGMGNDQVRFDLTVKALGDYEIVAPI
                     REIQKEHTAVRAYEQDYLKQRGFDVRAKQQAYTINENLLGVTLSGGEIDCWDVPGEDA
                     RGWCRARSEWPTEPLRVTIGFAGGEAKTLDGQRMAGHMLLSKLNHLFAQYGVGRGLYT
                     GDTTIGLKGRIVYEAPGLVALLTAHRALEEAVSSKQQNRFKPEVARRWVELVYEGFFH
                     DPLKGDLEAFLASSQRCVEGEVMLETQGGKVDAVAVRSNRILSAGGATYAQTADWGVV
                     EAEGFIRLYGMSSTLWGKVNRGPGTRDRGPGKSAPR"
     gene            7097..8347
                     /locus_tag="VFK00_12215"
     CDS             7097..8347
                     /locus_tag="VFK00_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7593753.1"
                     /translation="MPFVKLSGPRSRVPGPDNLLDATLRHLRELVSFDTRNPPRAIGT
                     GGIFDYLRAQLPGFHVQVGDHGAGAVSLLAVRGDPELLFNVHLDTVPDAPRWSASPFE
                     LRTTEDRAIGLGACDIKGAAAALLAVAGACEGDMALLFTTDEEGVDPRCVASFLEAFP
                     SPSGRRLREAPDEGSRSAGGHNNAEPSPEPVSREARGFHAVIVAEPTRCEAVLAHRGI
                     QSVCARFAGRAGHASIEQKPTDNALHQAVRWANAALDFAEAQAQRNFGDLRGLRFNLG
                     RIEGGIKANVVAPDAELRIGMRPLPSMDMDELLERFHTLADQAPVEFRETFRGPPLPS
                     ATNRAEADLAAARASATGLGLPVGPAVDFWTEASLFSQAGYTTFVYGPGDIAQAHSAD
                     EWVALAHLQRYADSIHRIVAEAQA"
     gene            8344..9732
                     /locus_tag="VFK00_12220"
     CDS             8344..9732
                     /locus_tag="VFK00_12220"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7593754.1"
                     /translation="MNTGQNTRQTIVRLLSAMGSAREIQQYLKRFSQLDVKRFAVVKV
                     GGAVLRDELADLASSLTFLQQVGLTPVVVHGAGPQLDDELVRAGIEKRTFEGLRVTPP
                     QALGIIRRVFHHENLKLVDALHAMGTRATSVVSGVFAASYVDREHCGLVGKVDRIESA
                     PIDASLRAGSIPVIASLGETEDGQILNINADCAANALVRSLQPYKIVFLTGTGGLLDG
                     AGRVIDSINLSSEYERLMAQPWLDGGMRLKLHQVADLLADLPPSSSVSITRPSELAKE
                     LFTHKGSGTLVRQGERVLRFDSWNGVDRAALRALIDSSFAPRKLVPDYFEQTRPHRVY
                     VSENYRAAMILLHQDGISYLDKFAVLEDAQGEGLGRAMWNVMRAENPRLFWRSRGGNR
                     IDAFYHQESDGCFKQERWKVFWYGLHGDFAAIARCVDHSAQKQPTLVECAGSDPVPEE
                     AGVEGANTGERS"
     gene            9729..10697
                     /gene="argC"
                     /locus_tag="VFK00_12225"
     CDS             9729..10697
                     /gene="argC"
                     /locus_tag="VFK00_12225"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7593755.1"
                     /translation="MNSGGCKSIGIVGARGHTGTELVRLISAHPALELAFVSSRGLGG
                     ERVADHDDSYHGELRYENLDPAAVAARHADVVVLALPNGKAAPFVEAIDKSKPDTLIV
                     DLSADHRFERTWYYGLPELTRGKWRGGRRISNPGCYATAIELTVAPLRDALAAPPVCF
                     GVSGYSGAGTTPSDRNHPEKLRDNLMPYALTGHVHEREASFHLGIPVEFMPHVAPHFR
                     GLTVTLNLYLSRAMRRDEVVARFADAYAKERLVRVVDDPPWVSRIAGRHHAEIGGFAM
                     SADGRRVVVVAALDNLLKGAATQAMQNINMASGSDELAGIPWRDEG"
     gene            10813..12102
                     /gene="argH"
                     /locus_tag="VFK00_12230"
     CDS             10813..12102
                     /gene="argH"
                     /locus_tag="VFK00_12230"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7593756.1"
                     /translation="MTDPLWMKPGTRINERIMRFLAGDDVLLDREFFLHDITASKAHV
                     EGLANIGVLRDGEAEALKRELDALADDFRSGGFVLDDRFEDGHSAIEARLTERLGDTG
                     RKVHTGRSRNDQILVATRLWLKEKLGALEAHCKASARICLDRAGNESLPMPGYTHLQR
                     AVVSSTSMWWAGFAEAFIDDAWRAHQTRDWIDANPLGSAAGYGVNLPLDRDHTTRSLG
                     FARMQVSVTYAQLSRGKFEMAVLEAIGSALLDVRRLAWDLSLFTTSEFGFVSLSPEYT
                     TGSSIMPNKRNPDVIELLRASYASVAAGRTEVEQLLSLPSGYQRDLQFSKGGIFPGVH
                     RGLMALELVPDLLARMQWNRAAMRAAIEPAMYATDVAIEQAASGVPFRDAYRAAAEAA
                     GAAGQGRTPEASLAARMSPGAAGDLRLDALQERLEAL"
     gene            12230..14617
                     /locus_tag="VFK00_12235"
     CDS             12230..14617
                     /locus_tag="VFK00_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7593757.1"
                     /translation="MPSLFRVKTARVVPIIAAALYCLLAAPAAAAADARPATRVQPSN
                     AQLASPEIERKVDALLAKMTLQEKIGQLVQYNDSVPASIDAGNPEMAGNPEAQQHIDA
                     MKLAAAGGLGSMLNIVGADRTRSFQQAAVEKSRLHIPILFGADVIHGFRTIYPVPLGL
                     AATWNPPLVTDLSRMAADEATTAGVRWFYSPMVDISRDARWGRTVEGAGEDPYLGAAM
                     ARAYIRGYQGDDLSRPDHVAASVKHFAAYGAAEAGREYNTTDMSDIRLRQVYLPPYKA
                     AVEAGAATMMSAFNALNGVPATANPYLLDEILRKEWGFNGFVVSDYTAVMELMNHGVA
                     LTPAIATRKAIEAGTDVDMMSHYYDTQLPKLLAEHKVSMATIDEAVRRVLRVKFALGL
                     FKHPYARGKEVTHAVPGHLPLARRAAEQSFVLLRNQSVAGGAPVLPLSSQAKTIALIG
                     PLADDAGQMIGAWGGASVDSDVVTVREALQARMQRDGGRLLYAKGTEIGGDSTAGFAA
                     ALAAAKQADVVVMALGESSAMSGEGGSRAHLDLPGNQQQLLERIVATGKPVVLLVFSG
                     RPLVLDWAAQHVPAIMETWFPGVQAGPAVAAVLFGDVAPSGKLPMSFPRAVGQEPLYY
                     NQFPTGRPPGDTDLGKPPVGGERYVSRYIDVPNSALFPFGWGLTYTTFAWSDVHVSRP
                     SVPLGEANQPGARDLVTVTATVKNTGSRSATEVAQLYVRNLGASVEQPLRSLKGFQRV
                     TLQPGESRQVTFEVGFAELSFWNAHSKQVVEPTHYTVWVGGSSLADQNAGFMVTP"
     gene            14775..15611
                     /locus_tag="VFK00_12240"
     CDS             14775..15611
                     /locus_tag="VFK00_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HET7593758.1"
                     /translation="MNVLEAVPRLRKPRVLEIAIAALIVVLAWSAFVTVGPGDRGVLM
                     TFGAVRDGVLAPGLHMKIPFVQTVKLMNVQIQKSETSETAASRDLQDVTTKVAVNWSI
                     TPADAAWVYQRLGDETQLTDKVIAPIVSNAVKAVAARYDAEQLVEKRDVVRDQIQQQI
                     VAALAHYKVQVQGVNITNFSFSEDYAKAIEQKQVAQQRAQQAEYDLARTKVQAQQEVA
                     QAQGQAQAQKLLQSTLTPQIIQLKAVDKWNGVLPQVTGGGAVPFIGDIAAGKATVSVP
                     AK"
     gene            complement(15637..16383)
                     /gene="phbB"
                     /locus_tag="VFK00_12245"
     CDS             complement(15637..16383)
                     /gene="phbB"
                     /locus_tag="VFK00_12245"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HET7593759.1"
                     /translation="MSKRVAVVTGGIGGLGTAICKRLAEDGRQVIAADLGNRQERLAA
                     FREETRDYNGSIVFAPADVANFDDCDALVTHATERYGSVDIVVNAAGITRDTSLKKMS
                     QSQWSELMHVNLDGVFNMCRNTVDGMTGRGFGRIVNISSVNGQTGQFGQTNYSAAKAG
                     MHGFTMALAREVAKKGVTVNSVSPGYCKTEMVMKVPEDIRAQIVAQIPVGRLGEPSEI
                     ARTVAFLTADDAGFITGANIPVNGGYFMSF"
     gene            complement(16513..17007)
                     /gene="phaR"
                     /locus_tag="VFK00_12250"
     CDS             complement(16513..17007)
                     /gene="phaR"
                     /locus_tag="VFK00_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HET7593760.1"
                     /translation="MASPRVIKKYPNRRLYDTKVSSYITLEEVRQLVLAGEHFEVRDA
                     KTNEDLTRAVLLQIIAEHEQHGQPIFSTTLLSQIIRFYGDAMQGFVGNYLENSLKVFL
                     DQQHKFRDQLNSLLGQTPWSMLNEATERNIELWKSLQPAAPAAGKPAAEPPSDDRKPA
                     RKRR"
     gene            complement(17136..17723)
                     /locus_tag="VFK00_12255"
     CDS             complement(17136..17723)
                     /locus_tag="VFK00_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HET7593761.1"
                     /translation="MDRGANISALGYGAFAVTLWLASMAPADWFSPVADAMLLQPLTI
                     VLGGCVLGVAGIFACVRGNTLDAVLFLTFAGYWWVGGLAQQRLAEDLRPSAGWLGWYY
                     IVWAFLVFCIWIAACKDGAARMLFTLGLCLSLLAYALAGWTRLDSLTVLGGYLGLVTA
                     VIGIYIAAAEMINETHGHVVLPLGESARDRRPGLS"
     gene            complement(17872..18996)
                     /locus_tag="VFK00_12260"
     CDS             complement(17872..18996)
                     /locus_tag="VFK00_12260"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7593762.1"
                     /translation="MEHWLYHTTWFGNSTFAWLVAAGGALLGYIVAHTAAAIIAAHLR
                     KLAARTHRRGLAIAADVAQATRGWLLFLIAIAIALDFLQFGATTTEGSAARHWTIADG
                     LHLLTYALIGIQVAFWVSALVVSWLRRSANRDGGEPANPVMLGVLTWVVQFVIWVTLV
                     LALLAEGGVQISAFVASLGVGGVAVALALQNVLGDLFASISIGLDKPFEVGEFIAFGT
                     DLGTVTKVGVKTTRIASLSGEELAISNSNLLKNLIHNYTRMQERRVVFGFRVPYGTSR
                     HDLQAIVDGARAAITNQEQTRLDRGHFRAFGDSGFEFEFVYYVLDPGYTLYCDIQQRI
                     NFDIMAVLEGLGVDFAVPARRLHGPAPRPSDVPEAADAPH"
     gene            complement(19033..20262)
                     /gene="kch"
                     /locus_tag="VFK00_12265"
     CDS             complement(19033..20262)
                     /gene="kch"
                     /locus_tag="VFK00_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HET7593763.1"
                     /translation="MLPSSTNLLRNRIQQLHQRVGASHWFPHVPLAILLALGGLWLLQ
                     TDLGGQWRPVLAQLLNPSRSGGLRPEYLPPLLIGAGMLIIGAGLLFRSRLAWAMALLL
                     AVAAAASMLFGQHPRAHLMLAYFLLVAALLAFAWRQFDRSSIAASTLFALTSVVLLVV
                     YATFGTYYLGADFKPQVRDLVTALYYAMVTMSTVGYGDITPQTPEAKLFTVSVIALGV
                     AVFATSLTAVIAPLATNSFQRIVNRKGIRMKRENHFVVIGDTPLALNTSRELARRGLA
                     VTRILGQAPSTQVVENVEVVVGEASDSDVLREAGADKAQAVLAMLDDDSENAFLVLAV
                     KELAGTARTVAAVNDAHHLARVQLVQPDVVIAPQILGGELTAMILSGETVTADFVMKR
                     VFQQVAGDHPTASTPTP"
     gene            20479..>21792
                     /locus_tag="VFK00_12270"
     CDS             20479..>21792
                     /locus_tag="VFK00_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7593764.1"
                     /translation="MKRAKLQRFGVALACLSLTCAAGAAAPSGDNHADADMLGFTASG
                     VAQEQSLEQRFDALLSASDMRAWLKQLSSEPNQVGSPHDKANAEFLLAQFRQWGWDAH
                     IETFDVLYPTPKQELVELVAPTHFKATLHEPPIEGDRTSTLSGALPPYNVYGADGDVT
                     GELVYVNYGMPDDYKELARLGVSVKGKIVIVRYGGGWRGLKPELAHEHGAVGCLIYSD
                     PRDDGYFHDDTYPEGPSRPPEGVQRGSVAKMQLYPGDPTTPGYGSTAGAKHVPVDQAK
                     TVLKIPVLPISYADATPLLKAMRGPVAPESWRGALPFTYHVGAGPAKVHMVVKSDWSR
                     KPVYDVIAKLKGSGDADQWVVRGNHHDGWVFGAFDPLAGTIALMGEAKAMGQLYRQGW
                     RPKRTIVYTSWDGEEPGLLGSTEWAEAHAKELQDKAVLYLNSDTNG"
BASE COUNT         3839 a         7028 c         7353 g         3572 t
ORIGIN      
        1 gatcgcgggc cccagcgtgt tcatctgcga cgaatgcgtc gagctttgca acgacatcat
       61 ccgcgaggaa ctggaggaaa aggccgcttc cgggcggacc cagctcccga agccgcgcga
      121 gatcatggag acgctcgacc agtacgtgat cgggcagacc cgcgccaaga agtcgctggc
      181 ggtggcggtg tacaaccact acaagcggat ggagtcgcgc gccaagaacg atgacatcga
      241 gctggccaag tccaacatcc tcctgatcgg accgaccggc agcggcaaga cgctgctcgc
      301 ggaaacgctg gcgcggatgc tcaacgtgcc gttcacgatc gccgatgcga ccacgctcac
      361 cgaagcgggt tacgtcggcg aggacgtcga gaacatcatc cagaagctcc tgcagaagtg
      421 cgactacgac gtcgagaagg cccagaccgg catcgtctac atcgacgaga tcgacaagat
      481 ctcgcgcaag agcgagaacc cgtcgatcac ccgcgacgtg tcgggcgagg gcgtgcagca
      541 ggcgctgctg aagctgatcg agggcaccat cgcctcggtg cccccgcagg gcggccgcaa
      601 gcacccgcag caggaatttc tgcaggtcga cacccgcaac atcctgttca tgtgcggcgg
      661 ggcgttctcg gggctggaga agatcatcca gcagcgctcc gaaaccaccg gcatcggctt
      721 cgccgccgag atccgcagca aggaacgccg ccagaacgtg gggaccctgc tcgcgaacgt
      781 cgagcccgag gacctggtca agtacgggct gatccccgag ttcgtcgggc gcctgccggt
      841 cgtcgccacc ctcgacgagt tggatgagga agcgctggtc aagatcctga ccgagccgaa
      901 aaacgcgatt accaagcagt tcaagaagat gttcgacatg gaaaatgtcg agatggagtt
      961 ccgcccggac gcgctcaccg cgattgccaa gcgcgcgatc aagcgcaaga caggtgcacg
     1021 cggcctgcgc acgattctcg aaggcgtgct gctcgacacg atgtacgaac tgccctcgct
     1081 ggaacacgtc agcaaggtcg tcgtggacga agccgtgatc aatggccagg ccgagcccta
     1141 cctgatctac cgcggcaacc tccagccgca ggcccgcgcc gagggcgacg ccgcctgacg
     1201 gaaaaccggg gtcagagtag agtttcgagg attgacccac cgggcaggca gtttcgtcag
     1261 ggaaactgta ctctgacccc ggttttctac cccggttctc cgagcacccg ctccgcgcgc
     1321 gccttgatgg tcttgaccat cgaggccagc ccgttggagc gcgtcggcga gagatgcttg
     1381 ccgagcccga tcgcgttgat gaagccggga tcggtgtcca ggatctcgcg cgcgcttcga
     1441 cccgaataca cccgcagcag cagcgcgacc aggcccgaaa cgatggccga gtcgctggcc
     1501 gcgcgcaggt cgagctgcgc ggcgtcgccg gaaaccacga tccacacctg cgactggcaa
     1561 ccgtcgactt tgttttccgg ggacatctcg gtgtcggtga gcggcggcag cttgcggccg
     1621 aggtcgatca ggtactggta gcgcccggtc cagtcgtcga agaagccgaa ctcatcgacg
     1681 atttcctgct gggcctcctg gatggaaatg tcattcatgg cgtggtgact ttccagcgcg
     1741 ttccctgcgg accgtcctcg atggcgatgc ccatcgctgc gagttcattg cggatttcgt
     1801 cggcgcgggc gaaatcgcgg gcagcgcggg cggcgcgacg cgcttcgatg agggtttcga
     1861 cacgcgtcga atccacctgt tccgcaccgc gcttgaacca ggcttcggga tcctgctgaa
     1921 gcaggccgag cagtgcgccg cccgcaagca gtgcagccct ggcttggctc cgttccgccg
     1981 gggaaaccgc acgtcgcgcg tcgtccgcga gcttggcgag ctcggccatc gcctctgggg
     2041 tattcagatc gtccaacagg gcattttcga ggttcgccgg cgagtcggca tcggcagctg
     2101 tcgcttcgac actggcgaga tcccggagcg tgccgtacaa ccgatccagc gtcgcgacgc
     2161 tctgttcgat cagcgcatcg gaccaatcca gcggctgccg gtaatgggcc gagagcaggg
     2221 cgtagcggat cgcttccggc gggtattgtt ccagcagcgc gtgcagttgc cggacattgc
     2281 cgacggactt gctcatcttg gcgccgccga aggtcagcat gccgttgtgg atccagaagc
     2341 gcgcgaagac ccggccaccg tgcgcgcagg tcgattgcgc gatttcgttc tcgtggtgcg
     2401 ggaacacgag gtcgacgccg ccggcgtgga tgtcgatggt ttcgccgagg tgcgcggccg
     2461 ccatcgccga gcactcgatg tgccaacccg ggcgtccgcg gccccagggg ctttcccagc
     2521 cgggcagctc aggcgtggac ggtttccaca acacgaaatc cgcgggattc cgcttgtacg
     2581 gcgcgacctc gacgcgcgag ccggcgatca gctcgtcggt cgatcgcccg gacagcgcgc
     2641 cgtagtcggc aaagctcgcg acgtcgaaca gcacgtggcc ctccgccacg tatgcgtggc
     2701 gactcgccag caatgcctcg cacatcgcga tgattgccgc gatgtgccgc gtggccatcg
     2761 gttcgacatc cggggccgcg acaccgagtt tcgcgatgtc ttcgcggtaa atcgccgcga
     2821 acttctcggc gatggcggcg atcggcaggc ctgcctcggc cgccgccgcg ttgatcttgt
     2881 catccacgtc ggtgatgttg cgcgcgtaca cgacgcgcgg gtatttgcga cgcagcagcc
     2941 gcgccagcac gtcgaacacg acgggaccgc gggcgttgcc gatgtgcacg tagctgtaca
     3001 cggtgggtcc gcacagatac atcgtcacgc gcgccggatc ccccggctcg aaaggctcga
     3061 tgcggcgggt cagcgagttg tagaggcgaa ggctcatccg gctgtgaaga tgttcgcgaa
     3121 ataatcaagc aattttatca ggttgcgatc attgcttgag cctttattca gcatgcggat
     3181 ggtccaatgg cacccatcac agatggaccc gcgggtcctg cgagcctccg gggggagcac
     3241 atcatgacca aaatcctggt ttcctcgctg ctgctcggtt tcgccgtggc ggccaacgcc
     3301 cagaactacg ggcctccacc gccgccggcg gccgcgcaag ccaccaacgt gcgatatgga
     3361 tgggccgagg tgttgcgggt cgacccggtg tacgccgccg tcgcggttcc gccgcagcag
     3421 gagtgctatc ccgaaacggt cacccgcgag gacaacaacc ataccggcgg gaccgtgctg
     3481 ggcgccatcg tcggcggcgt gctaggcaac accgtgggca agggcgacgg gcgcaaggcc
     3541 gcgacggtcg ccggtgcggt cgcgggcggc gcgatcggga accgggtctc ggcggcgcac
     3601 gatcgcagct ataccagcca gcaaacggtc tgtcgtccgg ttgaccgcta cgccccgcag
     3661 caacagatcg tgggctacga cgtcgagtac cgctatcgcg gcgacgtgta catgtcgcgc
     3721 ctgccgtatg atccgggcga gcggctacgg gtcaggatga cgatctcgcc ggcggactag
     3781 ggtggtctgg cgggatcgat catccgtgat gctgccccct tcagtgaatg tccaccgtat
     3841 cccgatgcgc tctggggccg acggctgccc cgtcaccgtt acgcagcgcg cccgttgcgc
     3901 atgcgcaaga ggtgtgcgac gattcgcttc catcgagtga acgcgagatg acccccaacg
     3961 cttgcgccca gcccttcatc ctcaccagcg cccgtgcggc cgccgggatg acgtcgcgcg
     4021 cgcgccgacc ggaatcacga ggtccggccg ggtaggcatt cacgcgcgtt gacgcaatat
     4081 cgaatgactg aaaaacccgg ccccgtgccg ggttttttgt ttcccgtcat cccggccatg
     4141 gattggacgg gccccggaat cagggcgggt aaagccgcca tccatggcca atttttcaca
     4201 aaacgcactc gaaccgtgac cctacgacac ttcatcacca cccaagactg gagccgcggc
     4261 gaaatcgacg cactcctcgc gcgcgccgcc gaattcaagc gttccccccc cgccggcgcg
     4321 ctcgcgggaa agtcgatcgc gctgctgttc ttcaacccgt cgatgcgtac acgcaccagc
     4381 ttcgagctgg gcgcgttcca gctcggtggc catgccgtgg tgttgtcacc cggcaaggac
     4441 gcatggccga tcgaattcga cgtgggcgcc gtgatggacg gggaagccga ggaacacgtt
     4501 gcggaagtgg cgcgcgtgct gtcgcgctac gtggatttga tcgcggtgcg tgcgttcccg
     4561 aagttcaggg attgggcaac ggatcgtgag gaccgcgtga tccgtgcctt tgcgcaatac
     4621 gccaccgtgc cggtgatcaa catggaaacc atcacgcacc cgtgccagga acttgcgcac
     4681 gcgctggcgc tgaaggaaca cctgggcgaa ttgcgtggca ggaagtacgt gctgacctgg
     4741 acgtaccacc cgaagccgct caacaccgcg gttgccaatt ccgcgttgct gatcgccacc
     4801 aagatgggca tggacgtgac gctgctgtgc cccacgccgg aatacgtgct ggacgaacgc
     4861 tacatggtgt tcggcgaggc caatgcgcgc gaaaacgggg gctcgctgcg cgtgacccac
     4921 gacatcgagg acgcctatcg cggtgcggac gtggtgtacg cgaaaagctg gggggcgctc
     4981 ccgtacttcg ggcgctggga tgcggaaaag ccgattcggg acgcgcatga acacttcatc
     5041 gtggacgaag cgaagatggc gctgacccat cgcgcagtat tcagccactg cctgcctctt
     5101 cgccgcaacg tcaaggccac cgatgccgtg atggatgcgc cgtactgcat cgcgatcgac
     5161 gaggccgaga accgcctgca cgtgcagaag gcggtgatgg cgacccttgc agccgaaccc
     5221 gagaaggaga catcgacatg acagaccatc cgcaccgcta tcgcgtggac gtcgaatgga
     5281 ccggcaatca cggcagcggc accgacggtt accgcaacta cggccgcgag cacgtgatcc
     5341 gcatcgaggg caaacccgag atcgcaggtt cgtccgatcc gaagttccgc ggtgatggcg
     5401 gcaagcacaa tcccgaagac atgctggtgg cagcgctatc cacctgccac atgctggcct
     5461 atctgcatgt ggccacgctt gcgggcgtgg tggtgaccgc gtacaccgac accgcggaag
     5521 gaaccatggt gaccgtagca gacggcggtc acttcaccga agtggtactg catccggtcg
     5581 tcacgatcac ggctgcaagc gatgcctcca aggctgaagc cgcgcacgag gacgcgcacc
     5641 acgcgtgctt cattgccgct tcggtcaatt tcccggtgcg ctgcgagccg agcaccgtgg
     5701 tggagcaggg accagactga tcgtctttcg attgttgttg gttcgctcca tcgaacggaa
     5761 atacctgccc atgacaaaga catccaagac cgctcgtccc cggttcccgg tccccggtcc
     5821 cgaaggcaag cacatcgtgc ttgccttcag tggcggcctc gacaccagct tctgcgtgcc
     5881 gtacctcgcg gagcgcggat gggccgtgca taccgtgttc gccgacaccg gcggcgtggg
     5941 cgccgcagag cacgcctacg tggaagcgcg tgcgaaagaa ctgggtgccg cctcgcacgt
     6001 caccgtcgac ggcggtccgg cgttgtggga tcacttcgtc aagccgttca tatgggcggg
     6061 ggagggctac cagggccaat acccgttgct ggtttcggat cgctacctga tcgtcgaagc
     6121 cgcgatcacg cgcgcgatgg agctcggcac gcgcgcgatc gcgcacggct gcaccgggat
     6181 gggcaacgac caggtccggt tcgacctcac cgtgaaggcg ctgggtgact acgagatcgt
     6241 ggcgccgatc cgcgagatcc agaaggagca cacggcggtc cgcgcgtacg aacaggatta
     6301 cctgaagcag cgggggttcg atgtccgcgc caaacagcag gcgtacacga tcaacgaaaa
     6361 cctgctgggc gtgaccttgt cggggggcga gatcgattgc tgggacgttc ccggcgagga
     6421 cgcgcgcggt tggtgcagag cgcgcagcga atggccgacg gaaccgctgc gggtgacgat
     6481 cggtttcgcc ggtggtgaag cgaaaacgct ggacggccaa cgcatggcag gacacatgtt
     6541 gttgtcgaaa ttgaaccacc tgttcgcgca atacggcgtc ggtcgcggtc tttacaccgg
     6601 cgataccacg atcggcctga agggacgcat cgtttacgaa gcgccgggcc tggtggcgct
     6661 gctgaccgcg caccgcgcgc tggaggaagc ggtatccagc aagcagcaga accgtttcaa
     6721 gccggaggtt gcgcgccggt gggtggaact cgtctacgaa gggttcttcc acgatccgct
     6781 gaagggcgac ctggaagcat tcctggcgtc gagccagcgc tgcgtcgagg gcgaagtcat
     6841 gctcgaaacg cagggcggga aggtggacgc ggtggccgtg aggtcgaacc gcatcctcag
     6901 cgccgggggc gcaacctacg cgcaaacggc ggattggggc gtggttgaag ccgagggatt
     6961 catcaggttg tacggcatga gcagcacgtt gtggggcaag gtgaaccggg gaccaggtac
     7021 cagggatcgg ggaccgggca agagcgcccc tcgctaagcg gcaccacctg ttgcatcgcc
     7081 atcggagtgt cacctgatgc ccttcgtcaa gctttccggt ccccggtccc gggtccccgg
     7141 ccccgacaac ctgctggacg caacgctccg gcacctccgt gagctggttt ccttcgatac
     7201 gcgcaacccg ccgcgtgcga tcggcacggg cggcattttc gattatctgc gcgcgcaact
     7261 gccgggtttc cacgtgcagg tgggcgatca cggtgctggg gcggtcagct tgctcgccgt
     7321 tcgcggcgat ccggagctgc tgttcaacgt gcacctcgac accgtgcctg acgcaccgcg
     7381 gtggagcgcc agtccgttcg aattgcgaac gacggaagat cgtgcgatcg gcctcggcgc
     7441 ctgtgacatc aagggtgcgg ccgcggcgct gctggcggtc gccggcgcat gtgaaggcga
     7501 catggcgttg ttgttcacca ccgacgagga gggcgtcgat ccccgttgcg ttgccagctt
     7561 tctggaagcc ttcccttctc cctccgggag aaggttgcgc gaagcgccgg atgagggttc
     7621 gcgcagcgct ggaggtcaca acaacgccga accctcaccc gagcccgtct cccgggaagc
     7681 gaggggcttt catgccgtca tcgtcgccga gcccacccgt tgcgaagcag tacttgcgca
     7741 ccgcggcatc cagtcggtgt gcgcgcgctt cgccgggcgc gcggggcatg catccatcga
     7801 gcagaagccg acggacaatg ccttgcacca ggcggtgcgc tgggccaacg ctgcgctgga
     7861 tttcgcggaa gcgcaggcgc agcggaattt cggcgatctg cggggcctgc gtttcaacct
     7921 cggacgcatc gaaggcggca tcaaggccaa cgtcgtcgca ccggatgcgg agttgcggat
     7981 cggcatgagg ccgttgccgt cgatggacat ggacgaactg ctcgagcgct tccacaccct
     8041 cgccgatcag gcgccggtgg agttccggga gacctttcga gggcctccgc tgccctccgc
     8101 gacgaacagg gcggaggcgg atcttgccgc tgcccgcgca tccgcaacgg ggctgggtct
     8161 gcccgtcggc cccgccgtcg atttctggac cgaggcgtcg ttgttctcgc aggccggcta
     8221 cacaaccttc gtgtacggcc ccggcgacat cgcccaggcg cacagcgccg acgaatgggt
     8281 ggcgcttgcg catttgcagc gatacgccga ctccatccat cgcatcgtcg cggaagccca
     8341 ggcatgaaca ccgggcagaa cacccgtcaa accatcgttc gcctgttgtc ggcgatgggc
     8401 agcgccaggg aaatccagca gtacctgaag cgcttttcac agctcgacgt caagcgtttt
     8461 gcggtggtca aggtgggcgg cgcggtgttg cgcgacgagt tggcggatct cgcgtcttcc
     8521 ctgacgttcc tgcagcaggt cggcttgacg ccggtcgtgg tgcatggtgc cggtccgcag
     8581 ctcgatgacg agctggtcag ggcgggcatc gagaagcgca cgttcgaagg attgcgcgtc
     8641 accccgccgc aggcgctcgg catcatccgc cgggtgttcc accacgagaa cctgaagttg
     8701 gtcgatgccc tgcacgcgat gggcacccgc gcgacctcgg tggtgtcagg agtcttcgcc
     8761 gccagttacg tcgatcgcga acactgcggc ctggtcggca aggtggatcg catcgagtcc
     8821 gctcccatcg atgccagcct gcgcgccggt tcgatcccgg tgatcgcgag ccttggcgaa
     8881 accgaagacg ggcagatcct gaacatcaac gccgactgcg cggcgaacgc actggtgaga
     8941 tcgttgcagc cgtacaagat cgtgttcctg accggtaccg gtgggctgct ggatggcgcg
     9001 ggccgggtga tcgattcgat caacctgtcg agcgagtacg aacggctgat ggcgcagccg
     9061 tggcttgacg ggggcatgcg gctgaagttg caccaggtcg ccgaccttct ggccgacctg
     9121 ccgccgagtt cctcggtgtc gattacgcgt ccttccgaac tcgcgaagga gttgtttacg
     9181 cacaaagggt cgggaacgct ggtgaggcag ggcgaaaggg tgctgcggtt cgattcctgg
     9241 aacggcgtgg accgcgcggc cctgcgcgcc ctgatcgatt ccagcttcgc gccgcgcaag
     9301 ctggtgccgg actatttcga gcaaacccgc ccgcatcggg tgtacgtcag cgagaactac
     9361 cgggcggcga tgatcctgct gcaccaggac ggcatcagct acctcgacaa gttcgcggtg
     9421 ctggaggacg cgcagggcga ggggctgggt cgcgcgatgt ggaacgtgat gcgggcggag
     9481 aatccacgcc tgttctggcg ctcccgcggc ggcaaccgca tcgacgcgtt ctaccaccag
     9541 gaatccgatg gttgtttcaa gcaggagcgc tggaaggtgt tctggtacgg attgcacggc
     9601 gacttcgccg cgatcgcccg ttgcgtcgac cacagcgcgc agaagcagcc caccctggtg
     9661 gaatgcgccg gctccgaccc cgttccggag gaggcggggg ttgagggtgc gaacaccggg
     9721 gagcggtcat gaacagcggc ggctgcaaat cgatcgggat cgtcggggcc cgtgggcaca
     9781 ccggcacgga gctggtccgg ctgatctcgg cgcacccggc gctggagctg gcgttcgtgt
     9841 catcgcgcgg cctgggcggg gagcgggttg cggaccacga cgacagctac cacggcgaat
     9901 tgcgctatga aaatctcgat ccggccgccg ttgcagccag gcacgcggac gtggtggtgc
     9961 tggcgctgcc caacggcaag gccgcgccgt tcgtggaagc catcgacaag tcgaagccgg
    10021 atacgctcat cgtggacctg tcggcggacc accggttcga gcgcacgtgg tattacggct
    10081 tgccggagct gacccgcggc aaatggcgcg gcggccgccg catcagcaat cccggctgct
    10141 atgcgaccgc gatcgaattg actgtcgcgc ctctcaggga tgcgctggcc gcgccgccgg
    10201 tgtgtttcgg ggtttcgggt tactccggtg ccggcaccac gccctcggac cggaaccatc
    10261 cggaaaagct tcgcgacaac ctgatgccgt atgccttgac cgggcacgta cacgaaaggg
    10321 aggcgtcgtt ccacctcggc atcccggtgg agttcatgcc gcatgtcgcg ccgcatttcc
    10381 gcgggctcac ggtgacgctc aacctgtacc tgtcgcgcgc catgcggcgc gacgaagtgg
    10441 tcgcgcgttt tgccgatgct tatgcgaaag aaaggctggt gcgcgtggtg gatgatccgc
    10501 cctgggtcag ccgcatcgcg ggccggcacc atgccgaaat cggcggcttc gcgatgtcgg
    10561 cggacggccg gcgcgtggtg gtcgtcgcgg cgctggacaa cttgctgaaa ggtgcggcga
    10621 cccaggcgat gcagaatatc aacatggcgt cgggatccga cgaactcgca gggattccct
    10681 ggagggacga ggggtgagta gccagggcac gctgagggcg ggttcgatgc gaacctgcga
    10741 cttgcgggaa cacagcatca atgccccctc gctgctcgtc tctcgcccct gttgccttcg
    10801 gagaatggag cgatgaccga tcccctctgg atgaagccgg gcacgcgcat caacgagcgc
    10861 atcatgcgct tcctcgcagg cgacgacgtg ctgctggatc gtgagttctt cctgcacgac
    10921 atcacggcca gcaaggcgca cgtggagggg ctcgcgaaca tcggcgtgtt gcgcgacggg
    10981 gaagccgagg cgctgaagcg cgagctcgac gcgttggctg acgatttccg cagcggtgga
    11041 ttcgtgctgg acgaccggtt cgaagatggc cattccgcga tcgaggcccg cctgaccgag
    11101 cggctgggcg acaccgggcg caaggtgcac accggacgca gccgcaacga ccagatcctg
    11161 gtggcgacac ggctgtggct gaaggagaaa ctcggggcgc tggaagcgca ctgcaaggcg
    11221 agcgcccgga tctgcctgga tcgcgcaggc aacgaatcgc tgccgatgcc gggctatacc
    11281 cacctgcagc gcgcggtggt gtcgtccacg tctatgtggt gggcaggctt cgcggaagcg
    11341 ttcatcgacg atgcgtggcg ggcgcaccag acgcgcgact ggatcgatgc caatccgctc
    11401 ggcagcgccg ccggctatgg cgtgaacctg cccctggacc gcgaccacac cacgcgctcg
    11461 ctcggcttcg cgcgcatgca ggtcagcgtg acctatgcgc aactctcgcg cggcaagttc
    11521 gagatggccg tgctggaggc gatcggcagc gcgctgctcg acgtgcgccg gctggcgtgg
    11581 gacttgtcgc tgttcaccac gtcggagttc ggtttcgtct cgttgtcgcc ggagtacacg
    11641 accggcagtt cgatcatgcc caacaagcgc aaccccgacg tgatcgagct gttgcgcgcc
    11701 agctacgcca gcgtcgccgc cgggcgcacc gaggtcgaac aattgctgtc gctgccctcc
    11761 ggctaccagc gcgacctgca attcagcaag ggcggcattt tccccggcgt gcatcgcgga
    11821 ttgatggccc tggaactggt gcccgatttg ctggcgcgca tgcagtggaa ccgggcggcg
    11881 atgcgcgcgg cgatcgagcc tgcgatgtac gccaccgatg tcgcgatcga acaggcggca
    11941 tcgggcgtgc cgttccgcga cgcctatcgc gccgccgccg aagccgccgg cgcggcgggg
    12001 cagggacgca cgccggaagc gagcctcgcc gcacgtatgt cgccgggcgc ggcgggtgac
    12061 ttgcggctgg atgcattgca ggagcgtttg gaagccttgt aggcatgggg tcccgtcagg
    12121 gacggaacca gtggccgtcg cgccagccgc aggaaaactg cagtgatgct gcgcttgccg
    12181 ggcgccatct ggaataatgg cctgtcgaga aaatccgtcc cgagaaatga tgccctcatt
    12241 gttccgcgtc aaaaccgcac gcgtggtgcc gatcatcgcg gccgcgctgt actgcctgct
    12301 cgctgcaccg gccgctgccg ccgccgatgc caggcccgct acgcgggtcc agccctccaa
    12361 tgcacaactg gcttcgccgg aaatcgagcg caaggtcgat gcactgctcg cgaagatgac
    12421 gttgcaggag aagatcggcc agttggtgca gtacaacgac agcgtcccgg cttccatcga
    12481 tgccggcaac ccggagatgg cgggcaaccc ggaggcacaa cagcatatcg acgccatgaa
    12541 actggcagcg gccgggggcc tggggtcgat gctcaacatc gtcggcgccg accgcacccg
    12601 gtccttccag caagccgcgg tggagaagag ccggctgcac atcccgatcc tgttcggcgc
    12661 ggatgtcatc cacggcttcc gcaccatcta tccggtgccg ctgggcctgg ccgccacgtg
    12721 gaatccgcca ctggtgacag atctgtcgcg catggccgcg gatgaagcca ccactgccgg
    12781 cgtgcgctgg ttctattcgc cgatggtgga catctcccgc gacgcccgtt ggggtcgcac
    12841 ggtcgaaggc gcgggcgagg atccgtacct cggggccgcg atggcgcgcg cctacatccg
    12901 tggctaccag ggcgacgacc tgtcccgtcc cgatcacgtc gcggcctcgg tcaagcattt
    12961 cgccgcctac ggcgccgccg aggccggccg cgagtacaac accaccgaca tgtccgacat
    13021 ccgcctgcgc caggtctacc tgccgccgta caaggccgcg gtcgaggccg gcgcggccac
    13081 gatgatgagc gccttcaatg cactcaacgg cgtgccggcc actgcaaacc cgtatctcct
    13141 ggacgaaatc ctgcgcaagg aatggggctt caacggcttc gtagtcagcg actacaccgc
    13201 ggtgatggaa ctgatgaacc acggcgttgc gttgaccccc gcgatcgcga cccggaaggc
    13261 catcgaagcc ggcaccgacg tcgacatgat gtcgcactac tacgacaccc agttgccgaa
    13321 gctcctcgcc gaacacaagg tatcgatggc aaccatcgac gaggccgtcc gccgcgtgtt
    13381 gcgggtgaag ttcgcgctgg gactgttcaa gcatccctac gcgcgcggca aggaagtgac
    13441 ccacgccgtg cctgggcacc tgccgctggc acgccgcgcg gcggagcaat cgttcgtgct
    13501 gttgcgcaac cagtccgttg ccggtggtgc gcctgtgctg ccgctgtcct cgcaggcgaa
    13561 aaccatcgcg ctcattggcc cgctggccga cgacgccggc cagatgatcg gcgcctgggg
    13621 tggcgcttcc gtcgactcgg acgtggtcac tgttcgcgaa gcgctgcaag cgcgcatgca
    13681 acgcgacggc ggccgcctgc tgtatgccaa gggaaccgaa atcggtggcg actccaccgc
    13741 cggcttcgcc gccgcactgg cggccgccaa acaggccgac gtggtggtga tggcgctggg
    13801 cgagtcgtcg gcgatgagcg gcgaaggcgg ctcgcgcgcg cacctggacc tgccggggaa
    13861 ccagcagcaa ctgctggaac gcatcgtcgc caccggcaaa ccggtggttc tgctggtgtt
    13921 ctcaggtcgg ccgttggtgc tggactgggc cgcccagcac gtgccggcaa tcatggaaac
    13981 ctggttcccc ggcgtgcagg ccggtccggc cgtcgccgcc gtattgttcg gcgacgttgc
    14041 gcccagcggc aagctgccta tgagttttcc gcgcgcggtt ggccaggagc cgctgtacta
    14101 caaccagttc cccaccggcc gtccgcccgg cgataccgac ctcggcaagc cgccggtcgg
    14161 cggcgaacgc tacgtctcgc gctacatcga cgtgcccaac tcggcgctgt tcccgttcgg
    14221 gtggggcttg acctacacca ccttcgcctg gtccgatgtg cacgtttcac gcccatcggt
    14281 gccgctgggg gaggccaacc agccgggcgc gagggacctg gtcaccgtga ccgcgacggt
    14341 gaagaacacc ggatcgcgca gcgcaaccga agtcgcgcaa ttgtacgtgc gcaacctggg
    14401 cgccagcgtc gaacagcccc tacgcagcct caagggattc cagcgggtca ccctccaacc
    14461 gggcgagtcg cgccaggtga ctttcgaggt gggcttcgcg gaactgtcgt tctggaacgc
    14521 gcacagcaaa caggtggtcg aacccacgca ctacacggtg tgggttggcg gcagttcgct
    14581 ggcggaccag aatgccggat tcatggttac gccatgagtg cgacgccaat ctgaggcaga
    14641 taccctgcgc gacagcatgt gcgggcgctc ggcgacggac cactgccgat ggttccccgt
    14701 ttcacggggc cgcgctggcg ctgcagtccc cgcttcgggt acgctgcggt cgatcaccgt
    14761 tgcgggggaa caccatgaac gtgctggaag cggtgccgcg gctgcgcaag ccgcgggtgc
    14821 tcgagatcgc gatcgcggcc ctgatcgtgg tgctggcgtg gtcggccttc gtcaccgtcg
    14881 gccccggtga tcgcggggtg ttgatgacgt tcggcgccgt ccgcgacggc gtgctcgcgc
    14941 ccggtctgca catgaaaatc ccgttcgtgc agacggtgaa gctgatgaac gtccagatcc
    15001 agaagtccga aacctcggag accgcggcca gtcgcgacct ccaggacgtc accaccaagg
    15061 tggccgtgaa ctggtcgatc acgccggccg atgccgcgtg ggtgtaccag cggctgggcg
    15121 acgagaccca gctcacggac aaggtaatcg cgccgatcgt gtcgaacgcc gtcaaggcag
    15181 tcgcggcgcg ctacgacgcc gaacaactgg tggaaaagcg cgacgtggtg cgcgaccaga
    15241 tccagcagca gatcgtggcc gcgctggcgc actacaaggt gcaggtccag ggcgtcaaca
    15301 tcaccaattt cagtttcagc gaggactatg ccaaggcaat cgagcagaag caggtcgccc
    15361 agcagcgggc acagcaggcc gaatacgatc tcgcccgcac caaggtccag gcccagcagg
    15421 aagtcgcgca ggcccagggc caggcgcagg cgcaaaagct cctgcaaagc accctgacgc
    15481 cgcaaatcat ccagttgaag gcagtcgaca agtggaacgg cgtgttgccg caggtgaccg
    15541 gcggtggtgc ggttccgttc atcggcgaca ttgccgccgg caaggcaacc gtgagcgtgc
    15601 cggcgaagta agcgaggccg gtttcgccac ggcagctcaa aacgacatga agtagccgcc
    15661 gttcaccggg atgttggctc cggtgatgaa gccggcgtcg tcggccgtga ggaaggccac
    15721 ggtacgggcg atttcggacg gctccccgag gcggccgacc gggatctgcg cgacgatttg
    15781 cgcgcggatg tcttccggca ctttcatcac catttcggtc ttgcagtagc cgggcgacac
    15841 gctgttgaca gtgacgccct tcttcgcaac ttcgcgcgcc agcgccatcg tgaagccatg
    15901 catgccggcc ttggcggccg aatagttggt ctggccgaac tggccggtct ggccgttgac
    15961 cgaagagatg ttgacgatgc gcccgaagcc acgcccggtc atgccatcga cggtgttgcg
    16021 gcacatgttg aacacgccat cgaggttgac gtgcatcagc tcgctccact gcgactggct
    16081 catcttcttg agcgaggtgt cacgggtgat cccggcggcg ttcaccacga tgtccacgct
    16141 tccatatctc tcggtggcgt gcgttaccag cgcatcgcag tcgtcgaaat tggcgacgtc
    16201 ggcaggcgca aacacgatgc tgccgttgta atcgcgggtc tcctcgcgga atgcggcgag
    16261 gcgttcctgg cggttgccga gatctgccgc gatgacctgg cggccatcct ccgcgagccg
    16321 cttgcagatc gcggttccca gtcccccgat gccgccggtg acgacggcta cacgtttgct
    16381 catcaagatc tccccgtatg tcgaaggccg ggaacggcct cgtcggccgc accgttcgcg
    16441 gacgttatgc cgcagagcgg catgtgctgc aacgcagcaa tccagcgatg gtacgaatcc
    16501 ggtggcgggg tgtcaacgcc gcttgcgcgc cggtttgcgg tcgtcggacg gcggttcggc
    16561 agcgggcttg ccggcggccg gcgcggccgg ctgcagcgac ttccatagct cgatgttgcg
    16621 ctcggtggct tcgttcaaca tcgaccacgg cgtctgcccg agcaggctgt tcaactggtc
    16681 gcggaacttg tgctgttggt ccaggaagac cttcaggctg ttctccaggt agttgcccac
    16741 gaagccctgc atggcgtcgc catagaagcg gatgatctgc gagagcaggg tggtcgagaa
    16801 gatcggctgg ccgtgctgtt cgtgctcggc gatgatctgg agcaggacgg cccgcgtcag
    16861 gtcctcgttg gtcttggcgt cccggacctc gaagtgctcg cccgccagca ccaactgccg
    16921 cacctcctcc agggtgatgt agctggacac cttggtgtcg tagaggcggc ggttcgggta
    16981 cttcttgatg acgcgcggtg atgccatgcg gcaaaagtag cacggggtgc ggcagcgcac
    17041 cagccagccg cccgggagcc gcgcgtgctc cggtcgcgcg ccgttccggg cgcgccgcgt
    17101 catcgaaagc gtggcttctg ccgatccgtc ttcggtcagg agagtccagg gcggcgatcg
    17161 cgagcgctct cgcccaacgg cagcacgacg tggccgtggg tttcgttgat catctctgcc
    17221 gccgcgatgt agatgccgat gacggccgtg acgagcccga ggtagccacc gaggaccgtc
    17281 aacgaatcga gccgcgtcca tcccgcgagt gcgtaggcca gcagcgacag gcacagtccg
    17341 agggtgaaca gcatccgtgc ggcaccgtcc ttgcaggcag cgatccagat gcagaacacc
    17401 aggaacgccc acacgatgta gtaccagcct agccacccgg ccgaaggacg caagtcttcc
    17461 gcgagcctct gctgggccag gccgccgacc caccagtacc cggcgaacgt gaggaacagg
    17521 acggcgtcca gcgtgttgcc gcgcacgcac gcgaagatgc cggcaacccc gagcacgcag
    17581 ccgcccagca cgatcgtcag tggctgcagc agcatcgcat ccgcgaccgg actgaaccag
    17641 tcggcaggcg ccatgctggc gagccacagg gtcacggcga atgcaccgta cccgagagcg
    17701 gaaatattcg cgcctctgtc catgccgacc tccgtccgca tgcgcaagcg gtgccacagg
    17761 gtcggcacgc gggtggtgca cgcgtctgat gattatcgac aagccatgtg tagaaacggt
    17821 cacgatcggg gtgcccttgt cacgctccgc gtggcgggct cgatgcaatc gtcagtgcgg
    17881 agcgtcggcg gcctccggaa cgtccgaggg gcgaggcgcg gggccatgca acctgcgcgc
    17941 gggcaccgcg aaatcgacgc ccagcccttc cagcaccgcc atgatgtcga agttgatgcg
    18001 ttgctggatg tcgcagtaga gggtgtagcc cggatcgagc acgtaataca cgaattcgaa
    18061 ctcgaagccg gagtcgccga atgcccggaa gtggccgcgg tcgaggcgcg tctgctcctg
    18121 gttggtgatc gcggcacgtg cgccatcgac gatcgcctgc aggtcgtggc gcgacgtgcc
    18181 gtacggcacc cggaaaccga acacgacgcg gcgctcctgc atgcgggtgt aattgtggat
    18241 caggttcttg agcaggttcg aattcgagat cgccagctcc tcgcccgaca acgaggcgat
    18301 ccgggtggtc ttcacgccga ccttggtgac ggtgccgagg tcggtcccga atgcgatgaa
    18361 ctcgccgacc tcgaacggct tgtcgagtcc gatgctgatc gatgcgaaca ggtctccgag
    18421 cacgttctgg agcgccagcg ccacggccac gccgccgacg ccgaggctcg cgacgaaggc
    18481 ggagatctgc acgccgccct cggccagcaa cgccagcacc agcgtcaccc agatcacgaa
    18541 ttgcacgacc caggtcagca cgccgagcat cacgggattg gccggctcgc ccccatcgcg
    18601 gttggccgag cgccggagcc atgacaccac cagcgcgctg acccagaatg caacctggat
    18661 gccgatcagt gcgtacgtta gcaagtgaag gccgtccgcg atcgtccagt gccgcgcggc
    18721 gcttccctca gtggtcgttg ccccgaattg gaggaagtcc agcgcgatcg cgatggcgat
    18781 caggaacaac agccagccgc gcgtggcctg ggcgacgtcc gccgcgatgg ccagtccgcg
    18841 ccgatgggtg cgcgcggcca gcttgcgcag gtgcgcggcg atgatcgcgg cggcggtgtg
    18901 cgccacgatg tagccgagca gggcaccccc ggcggcgacc agccaggcga acgtcgagtt
    18961 tccgaaccag gtggtgtggt agagccagtg ttccatggtc ggttccatgt ctcctgtcga
    19021 ggggtcgtgg cctcagggcg tgggggtgga ggccgtggga tggtcgcccg cgacctgctg
    19081 gaacacgcgc ttcatcacga aatcggcggt caccgtttcc ccgctgagga tcatcgcggt
    19141 gagttcgccg ccgagtattt gtggtgcgat caccacgtcg ggctgcacca gctgcacgcg
    19201 cgccaggtgg tgcgcgtcgt tgaccgccgc gacggtgcgc gccgtgcccg cgagttcctt
    19261 gaccgccagc accaggaagg cgttctccga atcatcgtcc agcatcgcca gcacggcctg
    19321 ggccttgtcg gcccccgctt cgcggagcac gtcggagtcg ctggcctcgc cgacgaccac
    19381 ctcgacgttc tcgacgactt gcgtggacgg cgcctggccg aggatccgcg tgaccgcaag
    19441 gccgcgccgc gcgagttcgc gactggtgtt gagcgcgagc ggcgtatcgc cgatcaccac
    19501 gaagtggttt tcgcgtttca tgcggatgcc ctttcgattg acgatgcgtt ggaaactgtt
    19561 cgtggccaat ggcgcgatca ccgcggtcag cgaggtcgcg aacaccgcga cgccgagggc
    19621 gatgaccgat accgtgaaca gcttcgcctc gggcgtctgc ggcgtgatgt caccgtagcc
    19681 gaccgtgctc atcgtgacca tcgcgtagta cagcgcggtc acgaggtccc ttacctgtgg
    19741 cttgaagtcc gcgccgagat aataggtgcc gaaggtcgca tacacgacca gcaggaccac
    19801 cgaggtcaac gcgaacagcg tgctggcggc gatgctgctg cggtcgaact ggcgccatgc
    19861 gaaggccagc agcgccgcca ccagcaggaa atacgcgagc atcaagtgcg cgcgcggatg
    19921 ttgcccgaac agcatgctcg ccgccgcggc caccgccagt agcagcgcca ttgcccatgc
    19981 cagccgcgaa cggaacaaca ggccggcgcc gatgatcagc atgccggcac cgatcaggag
    20041 cggcggcagg tactccggcc gcaagccacc gctccggctc gggttcagca actgcgcgag
    20101 gaccggccgc cattggcctc cgaggtcagt ttggagcagc cacagtccgc ccagtgccag
    20161 caggatcgcc agcggcacgt gcggaaacca gtgtgaagcg ccgacgcgct ggtgcagttg
    20221 ctggatgcgg ttgcgcagga ggttggtcga gctgggcagc atcggtgcgg tcgatcggct
    20281 ctcggcccgg ctgcggcgat accccgccgg cggatgccga acgatagcaa gccgtgccgc
    20341 gtccgtcgaa tcagcgggca gggcatgacg attgccctat gcaaggcggg gcgcggcggc
    20401 gtacaaagga gcgtttcacc tcgcgtgccg tccgtgtcga tggcgcgctg gcgtccacgt
    20461 cctctcggag gggttgcgat gaagcgagcg aagctgcagc gattcggggt ggcactggcg
    20521 tgcctgtcgt tgacatgtgc ggccggtgcg gcggccccct ccggcgacaa ccacgccgac
    20581 gcggacatgc tgggcttcac cgccagcggc gtcgcgcagg agcagagtct cgaacaacgc
    20641 ttcgacgcac tgctgagcgc cagcgacatg cgggcatggc tcaagcagtt gtcgtccgaa
    20701 ccgaaccagg tcggttcgcc ccacgacaag gccaacgccg aattcctgct cgcgcagttc
    20761 aggcagtggg gctgggacgc gcatatcgaa accttcgacg tgttgtatcc gacgccgaag
    20821 caggaactgg tggagctggt cgcgccgacg catttcaagg cgaccctgca cgaaccgccg
    20881 atcgagggcg accgcacgtc gacgctttcg ggggcgctgc cgccctacaa tgtgtacggc
    20941 gccgacggcg acgtcaccgg cgaactggtg tacgtcaact acggcatgcc cgacgactac
    21001 aaggaactcg cgcggctcgg cgtcagcgtc aagggcaaga tcgtgatcgt gcgctacggc
    21061 ggaggctggc gcggactgaa gcccgaactc gcgcacgagc acggcgcggt cggttgcctg
    21121 atctattccg atccgcgcga cgacggctat ttccacgacg acacctatcc cgagggacca
    21181 tcgcggccgc cggaaggcgt gcagcggggt tcggtcgcca agatgcagct ctatccgggc
    21241 gacccgacca cgccgggcta tggctcgacc gccggcgcca agcacgtgcc ggtggaccag
    21301 gccaagaccg tcctcaagat ccccgtgctg ccgatctcct atgccgacgc gacgccgctc
    21361 ctgaaggcga tgcgcggccc ggtcgcgccg gaaagctggc gcggcgcgct gccgttcacc
    21421 tatcacgtcg gcgccggacc ggccaaggtg cacatggtcg tgaagtccga ctggagccgg
    21481 aagccggtgt atgacgtgat cgccaagctc aagggttccg gtgacgccga ccagtgggtg
    21541 gtccgcggca accaccacga cggctgggtg ttcggcgcgt tcgatccgct cgcgggcacc
    21601 atcgcgctga tgggcgaggc caaggcgatg gggcagctct atcggcaagg ctggcggccg
    21661 aagcgcacga tcgtttacac gagctgggac ggcgaggaac ccggcctgct cggttcgacc
    21721 gagtgggcgg aggcgcacgc gaaggaactc caggacaagg ccgtgctgta cctcaattcg
    21781 gataccaacg gc
//
LOCUS       DASSNH010000323         7044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_558072, whole genome shotgun sequence.
ACCESSION   DASSNH010000323 DASSNH010000000
VERSION     DASSNH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558072"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /gene="ctaD"
                     /locus_tag="VFK00_12275"
     CDS             <1..1602
                     /gene="ctaD"
                     /locus_tag="VFK00_12275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7593765.1"
                     /translation="TAHAVHYEEHHQSFFERWFMSTNHKDIGTLYLLFSFLMLFVGGG
                     FAMLIRAELFEPGLQLMQPYFFNELTSMHGLVMIFGVIMPSFVGHANWMIPMQIGAPD
                     MALPRLNNLSFWILPFAFILLLSTFFMPGGAPAAGWTMYPPLVLQGGDSIAFMIFAIH
                     LMGISSILGSINIIATILNLRAPGLDLLKMPMFCWSWLITSFLVIAVMPVLAGAVTMM
                     LFDRFFGTNFFNAAGGGDPVMYQHVFWFFGHPEVYIMILPAFGIISEIVPTFARKPLF
                     GYKAMVYAIACIAFLSFIVWSHHMFTVGMPLGSEVFFMYATMLIAVPTGVKIFNWVTT
                     MWKGSLSFEMPMLWAIAFIVQFTVGGFSGVMMALVPADFQYNQTYFIVAHFHYVLAAG
                     ALFSIIGAVYYWLPKWSGNMYSGFWSKAHFWLSVIGINVLFFPQHFLGLAGMPRRIPD
                     YNVAFTDWNMVSSIGGFLFGATQLIFVGVVVHCVFFSKQKAAARSWEHAHGLEWTLPS
                     PAPHHSFTVPPVVDDTQLAHGDINH"
     gene            1816..1989
                     /locus_tag="VFK00_12280"
     CDS             1816..1989
                     /locus_tag="VFK00_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593766.1"
                     /translation="MGEETLEASTQDQAGAVMDALAHRKSGARRTAWIIGAIAIAFFI
                     ASLVQGHLFHIPG"
     gene            2105..3007
                     /locus_tag="VFK00_12285"
     CDS             2105..3007
                     /locus_tag="VFK00_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7593767.1"
                     /translation="MPMNQPEANVYYVPHKSRWPIFITLGMFMFMVGAATWLNGYERP
                     GQIVLAIGALTIIGIMFGWFGNVIGESIKGVFNPQVDTSFRMGMMWFIMSEVFFFAAF
                     FGGLFYIRTGVVPLLGGSEHGVGLLNGGQLITNQYLWPGFSATWPTNGPEALGGSFIT
                     TSPWGIPLINTLLLLSSSVTVTIAHHALRAGNRKRLIGFLGFTIVLGLLFLLGQAVEY
                     WESYVHFNMTLHTGIYGATFYILTGFHCLHVTVGTIMLCVMWLRCVRGHFSRENHFGF
                     EAVAWYWHFVDVVWLCLFLFVYIL"
     gene            3151..4971
                     /locus_tag="VFK00_12290"
     CDS             3151..4971
                     /locus_tag="VFK00_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HET7593768.1"
                     /translation="MVRGASRWLWLGAFALLACEGPARAGGCTLAKYGTLSIQMIGER
                     PTTLAKVNGAVTRFAIDTGAWFNFMSRANADALGLRLVPAPFGFRMGGIGGFAYAHIA
                     RIDDLGILDTDLHNVDFVVGGSDAGEVSLGANVLDFADLEIDLAQGKVTLFKPDGCRK
                     SALAYWVKGGGTYEVAELRPAVDPRDRRSFVDVTINGKSVRALLDSGAAVTLLGRRAA
                     ERVGIDLHGAGLEPAGRVGGLGAKTYQSWVVPINTFSVGTETIQHSKMLVMDGNFGDG
                     STGMVLGVDFMLAHHMYIANSQRKLYFTYNGGRVFSLDTVSIGAHESAASATSDAGGE
                     ARTAADHALRGQARLARGELANAHSDLDAAIRMDPGNADDHLIRARILLAQKQPDAAL
                     DDLDKALNLDPQNLDASLLRAQLRHDKKDEAGAAADLAAAQKLAPPGSMQSRSIASLY
                     VAIDEPISAVPLLDAWIRVHEDDASLGSALNERCWARALANRALPDALHDCRAAIKRD
                     GDLAAYLDSLGMVYLRMGDYAESIKAYRQALAQSPDAAWSHYGLGLAELHDGQADAGN
                     AELAIARSLDKEIDARAGKLGLVATEGSPVDEASGIHPSK"
     gene            complement(5100..5324)
                     /locus_tag="VFK00_12295"
     CDS             complement(5100..5324)
                     /locus_tag="VFK00_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HET7593769.1"
                     /translation="MSATALYKIVLVIVFLVVIFELGQALYFMMTDRSGSNRTAWALT
                     RRVLFSALLIVLIIVGIATGILHPHGVVVR"
     gene            5400..6134
                     /locus_tag="VFK00_12300"
     CDS             5400..6134
                     /locus_tag="VFK00_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HET7593770.1"
                     /translation="MQRPSVFAVLLTLVGVGVFCALGVWQLDRAAYKQTVLARFDHAA
                     AAPLESLAHALSDPAPNDYPHVEVGGHFEGDRVYFLDNQMRAGRLGVMVFVPFVPDGS
                     ARALLVNLGFLAKMGADSTTLPDVPPIPAGEVSLTGIYAPPPLPGLKLGGNPLGRQAT
                     WPKLVTWIDTGEIGADLHRKVYPHVLLLDPDPHSAYLRHWTANVMPPARHRAYALQWF
                     SFALAAIVMFFVLHRPRRGEAVTEEE"
     gene            6141..6689
                     /locus_tag="VFK00_12305"
     CDS             6141..6689
                     /locus_tag="VFK00_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7593771.1"
                     /translation="MDLRNRRRLQAGLIAAVFVAPVIVALVLTMGGWVPNGRSYGRAI
                     QPERDLSPMPVQRADGRSFAWSNNDAEWTLVALPGPRCGDQCLRKLDLVHRARIALGQ
                     HAGKLRLLYLGAPPEGAAAQGFGGVWMVATTPSHLLDDLRATTRDTVSAVLVTPEGKA
                     LTRYPANFDAEGLRQDLKKVVH"
     gene            6689..>7044
                     /locus_tag="VFK00_12310"
     CDS             6689..>7044
                     /locus_tag="VFK00_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7593772.1"
                     /translation="MATMSLPLRTLAWVACVFAFCVIVFGSFVRLSNAGLSCPDWPTC
                     YGRVTWPVKPAAVARADQAFPHRPVETHKAWREQSHRFLAGTLGLLVLAEALVATFRS
                     RLRLGLVIASVVLIGVG"
BASE COUNT         1141 a         2256 c         2288 g         1359 t
ORIGIN      
        1 acggcgcacg cggttcatta cgaagaacac caccagagct tcttcgagcg ctggttcatg
       61 tccaccaacc acaaggacat cgggacgctg tacctgctgt tctcgttcct gatgctgttc
      121 gtgggcggcg gcttcgcgat gctgatccgc gccgagctgt tcgagccggg cctgcaactg
      181 atgcagccgt acttcttcaa cgaactgacg tcgatgcacg ggctggtgat gatcttcggc
      241 gtgatcatgc cctcgttcgt cggccacgcg aactggatga tcccgatgca gatcggcgcg
      301 cccgacatgg cactgccgcg gctcaacaac ctgtcgttct ggatcctgcc gttcgcgttc
      361 atcctgctgc tgtcgacgtt cttcatgccg ggcggcgcgc cggccgcggg ctggacgatg
      421 tacccgccgc tggtgctgca gggcggcgac tcgatcgcgt tcatgatctt cgcgatccac
      481 ctgatgggga tttcctcgat cctcggctcg atcaacatca tcgcgacgat cctcaacctg
      541 cgcgcaccgg gcctcgatct gttgaagatg ccgatgttct gctggtcgtg gctgatcacc
      601 tcgttcctgg ttatcgcggt gatgccggtg ctggccggtg cggtcacgat gatgctcttc
      661 gaccgcttct tcggcaccaa cttcttcaac gccgcgggcg gcggcgaccc ggtgatgtac
      721 cagcacgtgt tctggttctt cgggcacccc gaggtctaca tcatgatcct gcccgcattc
      781 gggatcatct ccgagatcgt gccgaccttc gcgcgcaagc ccctgttcgg ctacaaggcg
      841 atggtgtacg cgatcgcctg catcgcgttc ctgtcgttca tcgtgtggtc gcaccacatg
      901 ttcacggtcg gcatgccgct cggcagcgaa gtcttcttca tgtacgcgac gatgctgatc
      961 gcggtgccca cgggcgtgaa gatcttcaac tgggtcacca cgatgtggaa gggctcgctc
     1021 agcttcgaga tgccgatgct gtgggcgatc gccttcatcg tgcagttcac ggtcggcggt
     1081 ttttcgggcg tgatgatggc gctggtgccg gccgacttcc agtacaacca gacctacttc
     1141 atcgtcgcgc acttccacta cgtgctggcc gccggcgcgc tgttctcgat catcggcgcg
     1201 gtctactact ggctgccgaa gtggtccggc aacatgtatt cgggtttctg gtccaaggcg
     1261 catttctggc tcagcgtgat cggcatcaac gtgctgttct tcccgcagca cttcctgggc
     1321 ctcgccggca tgccgcgccg catccccgac tacaacgtgg ctttcaccga ctggaacatg
     1381 gtgtcgtcga tcggcggctt cctgttcggg gcgacccagc tgatcttcgt cggcgtggtg
     1441 gtgcattgcg tgttcttctc gaagcagaag gcggcggcgc gctcgtggga gcatgcccac
     1501 ggcctcgagt ggacgctgcc gtcgccggcg ccgcaccaca gcttcacggt gccgccggtg
     1561 gtagacgaca cgcagctcgc gcatggagac atcaatcatt gatttttgcg atcatccatg
     1621 atcgctgttg taggcagtgt tgagaatggt tcggcgagtc aagtcacaag cactaccaca
     1681 gccatccttg gcctgacttt tcaccaacag catgcttcga tgggacgacc gcgagcatgc
     1741 agacatgagt agaggtacta gaaaggcctc catggcctga cttttcacga aagcatgctt
     1801 cgaactagca aaaaaatggg tgaagaaacg ctggaagcgt caacgcagga tcaggccggc
     1861 gcggtgatgg atgcgctcgc gcaccgcaag tccggtgcgc gccgcacggc atggatcatc
     1921 ggcgcgatcg cgatcgcgtt tttcatcgcc agcctggtgc aggggcatct cttccacatc
     1981 ccgggctgac atgcgagcga caaacccaca cagcggcggg cctgtggaac gccctcttgc
     2041 aagaccgcgg acaggttgtc cgtagccgaa acgacatcgc cgagcccgat taccgaccgg
     2101 aacattgccc atgaaccagc ccgaagccaa cgtctactac gtgccgcaca agagccgctg
     2161 gcccatcttc atcacgctcg gcatgttcat gttcatggtg ggcgccgcga cctggttgaa
     2221 cggctatgag cggccggggc agatcgtcct cgcgatcgga gcgttgacca tcatcgggat
     2281 catgttcggc tggttcggca acgtgatcgg cgaatcgatc aagggcgtct tcaacccgca
     2341 ggtcgatacc tcgttccgga tgggcatgat gtggttcatc atgtccgagg tgttcttttt
     2401 cgcggcgttc ttcggcggcc tgttctacat ccgcacgggc gtcgtgccgc tgctgggcgg
     2461 atcggaacac ggcgtcggcc tcttgaacgg cggccagttg atcacgaacc agtacctctg
     2521 gccgggattc tcggccacgt ggcccaccaa cgggccggaa gcgctgggtg gctcgttcat
     2581 caccacctcg ccgtggggca tcccgctgat caacaccctg ctgctgttgt catcgagcgt
     2641 cacggtgacg atcgcccacc atgcgctgcg cgcgggcaac cgcaagcgcc tgatcggctt
     2701 cctcggattc acgatcgtgc tgggcctgct gttcctgctc gggcaggccg tcgagtactg
     2761 ggaatcgtac gtccacttca acatgacgct gcacaccggc atctatggtg cgacgttcta
     2821 catcctcacc ggtttccact gcctgcacgt cacggtcggc accatcatgt tgtgcgtgat
     2881 gtggctgcgc tgcgtgcgtg gccacttcag tcgcgagaac cacttcgggt tcgaggcggt
     2941 ggcgtggtac tggcacttcg tcgacgtggt gtggctctgc ctgttcctgt tcgtgtacat
     3001 cctgtagctt cgtgccgcgg ccccgtacga cctttttccg ggttgccggg cagcgacacc
     3061 gcaatctctc tccggaagga gaggatgtca cgcagttcgt aaaggtcgtt cgttgggtca
     3121 aggcacggcc atccatggcc ggggggctcg atggtgcgcg gagcgtcacg gtggctttgg
     3181 ctgggcgcgt tcgccctgct tgcctgcgaa ggtcccgcgc gagccggcgg gtgcacgctc
     3241 gccaaatacg gcacgctctc catacagatg atcggcgaac ggcccaccac gctcgccaag
     3301 gtcaacggcg cggttacccg cttcgcgatc gacaccggcg cctggttcaa tttcatgtcg
     3361 cgcgccaatg ccgatgcgct gggactgagg ctcgtcccgg cgccattcgg gtttcgcatg
     3421 ggcggcatcg gcggcttcgc ctatgcgcac attgccagga tcgatgacct cggcatcctc
     3481 gataccgatt tgcacaacgt ggatttcgtg gtcggcgggt cggatgccgg tgaagtatcg
     3541 ctgggcgcca acgtgctcga tttcgcggat ctggaaatcg acctcgcaca gggcaaggtg
     3601 acgctgttca aaccggatgg ctgccgcaag tcggcgttgg cttattgggt caagggcggc
     3661 ggcacctacg aggttgccga attgcgcccc gccgtcgatc cgcgtgaccg acgcagcttt
     3721 gtcgacgtga cgatcaacgg caaatcggtt cgcgcgttgc tcgacagcgg ggccgccgtc
     3781 actctgcttg gccggcgcgc cgccgagcgc gtgggcatcg atctgcacgg agcggggctc
     3841 gagccagccg gccgtgtcgg aggcctcggc gccaagacat atcagtcgtg ggtcgtaccc
     3901 atcaacacgt tttccgtcgg caccgaaacg atccagcaca gcaaaatgct ggtgatggac
     3961 ggcaactttg gcgatggttc gaccgggatg gtgctgggtg tcgatttcat gctggcccat
     4021 cacatgtata tcgccaacag ccagcgcaag ctctatttca cgtacaacgg cggccgggta
     4081 ttttcgctgg ataccgtctc gatcggagcg catgaatcgg cggcttccgc gaccagcgat
     4141 gcgggcggcg aagcgaggac cgcggccgat catgcgttgc gcggccaagc gcgtctggcg
     4201 cgcggcgaac tcgcgaacgc gcattccgac ctggatgcgg ccatccggat ggacccgggg
     4261 aatgcggacg atcacttgat acgcgcccgc atcctgcttg cccagaagca gcccgacgcg
     4321 gcgctggacg acctggacaa ggcgctgaac ctcgatcccc aaaacctcga tgcatcactc
     4381 ctgcgtgccc aattgcgcca cgacaaaaaa gacgaggccg gtgcggcggc ggatctcgcg
     4441 gcagcgcaaa agctcgcgcc gcccggttcg atgcaatcgc gttccatcgc atcgctctat
     4501 gtggccatcg acgagccgat cagcgccgtg cccttgctcg acgcatggat tcgggtacat
     4561 gaggacgacg cttcgctcgg cagcgcgctg aacgaacgtt gctgggcacg cgcgctggcc
     4621 aaccgggcgt tgccggatgc gctgcacgac tgccgcgcgg ccatcaagcg cgacggtgac
     4681 ctcgcggcct atctggacag cctgggcatg gtgtatctgc ggatgggcga ctacgccgaa
     4741 tcgatcaagg cctatcgcca ggccctcgct caatcgccgg acgcggcttg gtcgcattac
     4801 gggctgggcc tggccgaatt gcacgacgga caggccgacg cgggcaacgc cgagctggca
     4861 attgcgcgtt ccctggacaa ggaaatcgac gcacgtgcgg gcaagctcgg cctggttgcg
     4921 acagaagggt cgccggtgga cgaggcatcc ggtatacatc cttcgaaata gcgttgcgcc
     4981 agggcgggcg ttcgacggcg atcttccttg cggtcgaacg ggtgccgtcg aatcggcgct
     5041 tcgggggcgc cacatcgcat gaccgagttt gcgataattc cggttcccgc tcgccgttct
     5101 cagcgcacca ccaccccgtg cgggtgcagg atgccggtgg cgatccccac gatgatcagg
     5161 acgatcagga gggccgagaa cagcacgcgc cgcgtcaacg cccaggccgt gcggttgctg
     5221 ccgctgcggt ccgtcatcat gaagtacaaa gcttgcccca gttcgaagat caccaccaga
     5281 aacacgatga ccaggacgat cttgtacagg gcggtggcgg acatggcgag cgcgcggccg
     5341 gaaaccgcaa ttatagcgag acgtcgcgag cagccggcgc cggcccggcc atgatgcgga
     5401 tgcaacgccc cagcgtgttc gcggtgctgc taaccctcgt cggcgtcggg gtgttttgcg
     5461 cgctgggggt ctggcagctc gaccgcgcgg cttacaagca gaccgtgctc gcgcgcttcg
     5521 accacgccgc ggccgcgcca ctggaatccc tggcgcatgc gctgtcggat ccggcgccga
     5581 atgattatcc gcacgtggaa gtcggcggcc acttcgaggg ggaccgggtg tatttcctgg
     5641 acaaccagat gcgcgccggg cggctcgggg tgatggtgtt cgtgccgttc gtgccggacg
     5701 gttcggcgcg cgcgctgctc gtgaacctcg gctttctcgc caagatgggt gccgattcga
     5761 ccacccttcc ggacgtgccg ccgattcccg ccggcgaagt cagcctgacc gggatctacg
     5821 cgccaccgcc gctgcccggc ctgaagctcg gcggcaatcc gctgggccgc caggcgacct
     5881 ggcccaagct cgtgacctgg atcgacaccg gcgagatcgg cgcggacctg caccgcaagg
     5941 tgtatccgca tgtattgttg ctggatcccg atccgcacag cgcctacctg cgccactgga
     6001 cggcgaacgt gatgccgccc gcgcggcatc gcgcctacgc gctgcagtgg ttcagtttcg
     6061 cgctcgccgc gatcgtgatg tttttcgtgc tgcatcgccc caggcgcggt gaagccgtca
     6121 ccgaagagga atagagtccg atggatctgc gtaaccggcg acgcctgcag gcagggctga
     6181 tcgcagcggt cttcgtggcg cccgtgatcg tggcgctggt gctgacgatg ggcgggtggg
     6241 tgccgaacgg ccgcagctac ggacgggcaa tccagcccga gcgcgacctc tcccccatgc
     6301 cggtgcagcg ggccgacggc cgttcgttcg catggagcaa caacgacgcg gaatggacgc
     6361 tggtcgcgct cccgggcccg cgttgcggcg atcagtgcct gcgcaagctc gacctggtgc
     6421 accgcgcgcg gatcgcgctc gggcagcacg ccggcaagct gcgcctgctg tacctcggcg
     6481 cgccgccgga gggcgcggcg gcgcagggct tcggcggggt gtggatggtc gcgaccacgc
     6541 cttcgcacct gctggatgat cttcgcgcaa ccacgcgcga tacggtcagc gcggtgctgg
     6601 tgacgccgga gggcaaggcg ctcacgcgct atccggcgaa tttcgatgcc gagggcctgc
     6661 gccaggatct caagaaggtg gtgcactgat ggcgacgatg tcgctgccgc ttcgtaccct
     6721 ggcctgggtc gcatgcgtgt tcgcgttctg cgtgatcgtg ttcggatcgt tcgtgcggct
     6781 gtcgaacgcc gggctgtcct gcccggactg gccgacctgc tacggccgcg tcacctggcc
     6841 ggtcaagccg gccgcggtgg ccagggccga ccaggctttc ccccatcggc ccgtggaaac
     6901 gcacaaggcc tggcgcgagc agagccaccg gttcctcgcc ggcaccctgg gcctgctggt
     6961 gctggccgag gcgctggttg cgacgttccg gagcaggctg cgcctcgggc tggtgatcgc
     7021 atccgtggtc ctgatcggcg tcgg
//
LOCUS       DASSNH010000324        10319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_917157, whole genome shotgun sequence.
ACCESSION   DASSNH010000324 DASSNH010000000
VERSION     DASSNH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917157"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /gene="tolB"
                     /locus_tag="VFK00_12315"
     CDS             complement(<1..1209)
                     /gene="tolB"
                     /locus_tag="VFK00_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET7593773.1"
                     /translation="MRTAKILLAMGLTLFAALFAVQAAAQGLTVQIVNGVPSAIPVTV
                     VPFGQQGSGPASPVDIAQVVGMDLNRCGKFRTLPTGEIVESPTTGSQIKFATWQQLKQ
                     DYIVVGRTAYDGSVLKAEFELWDVNKQQQLLGLSITGQGSDLRRVAHQIADQIYQKII
                     GVRGAFDTRIAYVTMVGLGQNAQYTLVVADSDGYNPQTVVRSHEALLSPAWSPDGREI
                     AYVSFESGDSAIYIQNIATGARRVVSARPGINGAPRFSPDGQRLAVSLSFQGNPEIYT
                     LDLGTGALTRLTHNLAIDTEPSWMPDGQNIVFTSDRSGKPQLYQIPADGGTPQRVTFQ
                     GQWNGDVSVSYDGKKLAMVQGSGNVYRIAVMDRDLGGQETFVSPGNLDESPSWAPNAS
                     MLLYAATEGPR"
     gene            complement(1224..2108)
                     /gene="tolA"
                     /locus_tag="VFK00_12320"
     CDS             complement(1224..2108)
                     /gene="tolA"
                     /locus_tag="VFK00_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET7593774.1"
                     /translation="MLLHLGIVGFLALAMLNCTSWENVADFLRLPEAMRPVTCTRVVR
                     LEGPVIEATLVGPAAAPPPPAARHAQKASPEHEQKAQPKPKPPPPAPVRVLPKPVPQP
                     QLKDQEKVAQVAEEQAEKERQAQEEQHRQRMAELTRQKEAERILKELAKAKAESEAQQ
                     RRVNLEKQKAQQLADLHKAQESENAENLPPAKQAVTGQAGTDKAAYVAALQNAITQNW
                     LRPDNIPPGVVCPVKITQIPGGQVISAKVQPGCPFNDVAKASVENAVLRSSPLPYKGF
                     EKEFKRDIIFEFKVTSPE"
     gene            complement(2137..2592)
                     /gene="tolR"
                     /locus_tag="VFK00_12325"
     CDS             complement(2137..2592)
                     /gene="tolR"
                     /locus_tag="VFK00_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HET7593775.1"
                     /translation="MAMQHRGRRRRRMAEINIVPYIDVMLVLLIIFMVTAPMLNLGAD
                     IQLPQSAAKALQDEKKPVLVTVDEQGQVFLTLGSNAREQVDDDTLVKKVSAFVKEDPK
                     VSVLLGGDKRVDYGRVNQVLGLLQQAGVAKVGLMSQPTTAVVPADHGKR"
     gene            complement(2608..3324)
                     /gene="tolQ"
                     /locus_tag="VFK00_12330"
     CDS             complement(2608..3324)
                     /gene="tolQ"
                     /locus_tag="VFK00_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET7593776.1"
                     /translation="MNGSINIFDLVIHASWPVKLVLLILLIFSVTSWVIIFRKKAMLD
                     ASTRDADGFEERFWSGVDLAALFREVSNRAGEAGGLAAVFESGFREFVRQRQRGGEDR
                     RAVLESSERAMRVAGTREVEKLERNLEYLANVGSISTYVGLFGTVWGIMIAFQGLGSL
                     KQATIATVAPGISEALVATAMGLFAAIPAVWAYNRYSTRLERITLRYETFQEEFSSVL
                     HRQMQSDAPAAPGRAEAQLR"
     gene            complement(3332..3826)
                     /gene="ybgC"
                     /locus_tag="VFK00_12335"
     CDS             complement(3332..3826)
                     /gene="ybgC"
                     /locus_tag="VFK00_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HET7593777.1"
                     /translation="MFPGSSESLARIFGKHHSDPGFAAQPAFSWRIRVYWEDTDAGGV
                     VYHAGYLRFLERARSEWMRSAGLGQEALRDAHGIAFVVRDLGIAYDKPARLDDEVDAT
                     VHVERVRSASIDFAQSVLRVADGVTLARAQVRVACIDASTFTPVRIPAGIMVLIQSAC
                     SNKD"
     gene            complement(3826..4872)
                     /gene="ruvB"
                     /locus_tag="VFK00_12340"
     CDS             complement(3826..4872)
                     /gene="ruvB"
                     /locus_tag="VFK00_12340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7593778.1"
                     /translation="MSESRVVDAAETRDEEVLDASIRPRTLAEYVGQATLREQLSLSI
                     EAAKKRGDALDHVLVFGPPGLGKTTISHVIANELGVNLRSTSGPVLERAGDLAALLTN
                     LQPRDVLFIDEIHRLSPVVEEVLYPALEDFQIDIMLGEGPAARSIKLDLPPFTLVGAT
                     TRAGMLTSPLRDRFGIVHRLEFYTVPELAAIVRRAARILEIACDEEGADEIARRARGT
                     PRVANRLLRRVRDYAEVRAGGRITREAASAALDMLRVDGGGFDELDRRLLRLVLEQFE
                     GGPVGVESLAAALAEDRDTIEDVLEPYLIQQGLLARTARGRIATAKAWRHFGLKPPRG
                     SAPAMNLFDTQGSE"
     gene            complement(4935..5438)
                     /locus_tag="VFK00_12345"
     CDS             complement(4935..5438)
                     /locus_tag="VFK00_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET7593779.1"
                     /translation="MPEKKTIERAKKDKREGKSASTQAGEFVREEFEHIREGKHGARS
                     PEQAIAIGLSEARRAGVDLPPPKKGKASEKTIKQAERDRKAANKGTSRKVSAKRSRAT
                     RKALKREGHSAASKKAVARHARKAARKRSAGERSAAAKKAARTKGASERSAAAKKAAR
                     TRANRSR"
     gene            complement(5578..7482)
                     /locus_tag="VFK00_12350"
     CDS             complement(5578..7482)
                     /locus_tag="VFK00_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HET7593780.1"
                     /translation="MTDQDAAAAPSNDAGKKSLRVMVVGAIGIVFGDLGTSPLYTMSE
                     AFGYEHGMPVTTFNVLGVLSLVLWTLLIVVALKYVTFIMRADNKGEGGIMAMMALAQR
                     ATRQYPRTRKLVVLAAVLGAALFFGDGVITPSITVLSAVEGLEVAAPALSHWVVWIAV
                     IVLVAVFLVQKRGTGKVGLVFGPVMCIWFVALGLIGLYNIWQHPLVLRAISPSYGVNF
                     FMHHGAGGFLVLGAVVLCVTGAEALYADMGHFGRKPIRLAWYGFVMPGLMLNYFGQGA
                     LLVHNPAAASNPFYLAVPEWGLYPMIALATAAAVIASQAVIAGSFSIMRQAVQLGFLP
                     RMPIVHTSNEEVGQIYLPWVNRLLMVLVVLVVLGFGSSQALAGAYGIAVTGTMAIDTV
                     LFVIVAWRMWKWHPLLVLAVLVFFLTIDLSFFGANTLKIQDGGWFPLVLGLAVFAILT
                     TWRRGRELVLRQLKHSGLALAPFIANLAEHPPTRVEGTAIFLVSDPDAVPHALLHNLK
                     HNKVLHTCNVILNVETLETPYADPDERIELTDLGHDFQKLTLRFGFADDPDVPAALHQ
                     CARRGLDIKMMDTTFFLSRETIVAADRAGMALWRDKLFAFMARNALPATAFFSIPGNR
                     LIELGAQVEI"
     gene            complement(7479..8069)
                     /gene="ruvA"
                     /locus_tag="VFK00_12355"
     CDS             complement(7479..8069)
                     /gene="ruvA"
                     /locus_tag="VFK00_12355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7593781.1"
                     /translation="MIGRLRGTLIAKRPPWVLVEVGGVGYEMEVPMSTLYDLPDVGSE
                     VVLLTHYAHKEDTVALYGFLREDERILFRDLQKVSGIGARISLAVLSGVSTNEFARLV
                     QAGDVGALTRVPGIGKKTAERIVVELRDRVAGALTAVSPESAPHPRDARGEAEAALQQ
                     LGYKPAEAARLAANAAAEGGDAETIIRKALKAALGR"
     gene            complement(8131..8694)
                     /gene="ruvC"
                     /locus_tag="VFK00_12360"
     CDS             complement(8131..8694)
                     /gene="ruvC"
                     /locus_tag="VFK00_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET7593782.1"
                     /translation="MPASVPRSPFPVPHSVRILGIDPGSQRTGVGIIEADATGRLRCV
                     VYATLRVAGEETFPLRLKRIYDELGAIIDAEAPVEAAVERVFMAKNADSALKLGQARG
                     AAICAAVGRGLVIHEYAPNAIKQAVVGKGHAGKEQVQHMVGILLNMRGPLQADAADAL
                     AIAITHAHLRASEARTGIAAAAWGRRR"
     gene            complement(8804..9523)
                     /locus_tag="VFK00_12365"
     CDS             complement(8804..9523)
                     /locus_tag="VFK00_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7593783.1"
                     /translation="MGRGPSIAGRKNAQDAKRGQVFTKLVREIMVAARAGGGDVNGNA
                     QLRTAVDKAIAASMPKDNIERAIKKATGELEGVTYEELHFEGYAPGGVAVLVECLTDN
                     RQRTISDVRHAFTKFGGNLGTDGSVAYLFRKIGVLSYAGGADEEKLTDAAIMAGAEDI
                     KVWPEDGQIDVTTAPESFEAVRESMEKAGLKPDHAEVTMRAEVDVPVADDQVEAVQGL
                     LARLEELDDVQAVHSNADLPQ"
     gene            complement(9802..>10319)
                     /locus_tag="VFK00_12370"
     CDS             complement(9802..>10319)
                     /locus_tag="VFK00_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET7593784.1"
                     /translation="VADGWKPLWVTDFPMFEYDGERARHVALHHPFTAPKVDDIEALK
                     ADPARAVSRGYDMVLNGAEVGGGSIRIHRPEMQSAVFDLLGIDEREARAKFGFLLDAL
                     KFGAPPHGGIAFGIDRLAMLVGGTEAIRDVIAFPKTTSAQDLMTDAPSTIDEKQLAEL
                     HVQVRETGSRD"
BASE COUNT         1636 a         3526 c         3362 g         1795 t
ORIGIN      
        1 gcgtggtccc tcggtggctg catacagcag catgctggcg ttgggcgccc agcttggtga
       61 ttcgtcgaga ttacccgggg aaacgaacgt ctcctggccg ccgaggtcgc ggtccatcac
      121 tgcaatacga tacacgtttc cactgccctg caccatggcg agcttctttc cgtcgtagct
      181 gaccgacacg tcgccgttcc actgtccctg gaaagtgacg cgctggggcg tgccgccgtc
      241 agcaggaatc tggtagagct gcggcttgcc ggaacgatcg gaggtgaaga ctatgttctg
      301 gccatcgggc atccacgaag gctcggtatc gatcgcgagg ttgtgggtca gccgggtgag
      361 cgcgccggtg ccgaggtcga gggtgtagat ttccggattg ccctggaacg acaggctcac
      421 cgccagccgc tggccgtccg gcgaaaaccg cggggcgccg ttgatgccgg ggcgggcgga
      481 aaccacccgc cgcgcgccgg tcgcgatgtt ctggatgtag atcgccgaat cgccgctctc
      541 gaaggacacg taggcgatct cccgcccgtc gggcgaccat gccggcgaca acagggcttc
      601 gtgcgaacgc accacggtct gggggttgta gccgtccgaa tcggcgacca ccagcgtgta
      661 ctgcgcgttc tggccgaggc ccaccatggt cacgtaggcg atccgggtgt cgaacgcgcc
      721 gcgcacgccg atgatcttct ggtaaatctg gtcggcgatc tggtgcgcga cccggcgaag
      781 gtcgctgccc tgccccgtga tcgacagccc gaggagctgc tgctgcttgt tgacgtccca
      841 gagctcgaac tcggctttca ggacgctacc gtcataagcg gtgcggccga ccacgatgta
      901 gtcctgcttc aactgctgcc aggtcgcgaa cttgatctgc gagccggtgg tcggcgactc
      961 gacgatttcc ccggtgggca gcgtgcggaa cttgccgcag cggttcagat ccatgcccac
     1021 gacctgggcg atgtcgaccg gtgaagccgg cccggaaccc tgctggccga acggcaccac
     1081 cgtgaccggg attgccgaag gtacgccgtt gacgatctgg acggtcaggc cttgcgccgc
     1141 ggcctgcacc gcgaacagcg cggcgaacag cgtgaggccc atggcgagca ggattttggc
     1201 agttcgcatg gttggacgct tccttattct ggcgaagtca ctttgaactc gaagatgatg
     1261 tcgcgtttga attccttttc aaatcccttg tagggcagcg gcgaagaacg cagcaccgca
     1321 ttctccaccg aggctttggc cacgtcgttg aagggacagc cgggctgtac tttggcagaa
     1381 ataacctgac cccccggtat ctgcgtgatt ttcaccggac acaccacgcc cggcgggatg
     1441 ttgtccggac gcaaccagtt ctgcgtgatg gcattctgca aggccgccac gtaggcggcc
     1501 ttgtcggtgc ccgcctgccc cgtcacggcc tgcttggcgg gcggcaggtt ttcggcattc
     1561 tcgctttcct gcgccttgtg caggtccgcc agttgctggg ccttctgctt ttccaggttg
     1621 acccggcgct gctgggcctc gctctcggcc ttggccttgg cgagctcttt caggatccgc
     1681 tcggcctcct tctgccgggt gagttcggcc atccgctgcc tgtgctgctc ttcctgggcc
     1741 tgcctctctt tttcggcctg ttcctcggcg acctgcgcga ccttttcctg gtccttcagt
     1801 tgcggctgcg gcaccggctt cggcaacacc cgcaccggag cgggaggtgg tggcttcggc
     1861 ttgggctggg ccttttgctc gtgctccggg gacgccttct gcgcgtgcct ggcggcgggc
     1921 ggcggcggtg cggcggccgg tcccaccagg gtcgcctcga tcaccggtcc ctccaggcgc
     1981 accacgcgcg tgcaggtcac aggccgcatg gcctccggca ggcgcaggaa gtcggcgacg
     2041 ttttcccaac tggtgcagtt gagcatcgcc agggccagga agccgacgat cccgaggtgc
     2101 agcagcacgg agtaaacgac cgcgcgtggg gtggcgtcag cgctttccat ggtccgcggg
     2161 aaccactgcg gtcgtcggtt ggctcatcag cccgaccttg gcaacgccgg cctgctggag
     2221 caggccgagc acctggttga cccgtccgta gtcgacgcgc ttgtcgccgc cgagcagcac
     2281 ggacaccttc ggatcttctt tcacgaacgc cgaaaccttc ttgacgaggg tgtcgtcatc
     2341 gacctgctcg cgcgcgttgc tgccgagggt gaggaacacc tggccctgct cgtcgaccgt
     2401 caccagcaca ggcttctttt cgtcctgcag ggccttggcg gccgactgcg gcaactggat
     2461 gtcggcgccc aggttcagca tcggcgcggt caccatgaag atgatcagca gcaccagcat
     2521 gacgtcgatg tacggaacga tgttgatttc cgccatcctg cgacggcggc ggcctcgatg
     2581 ttgcattgcc atgggctggt ccctcgctca gcgcaactgc gcttccgcgc gccccggcgc
     2641 cgcgggcgca tcgctctgca tctgccgatg cagcaccgac gagaactcct cctggaaggt
     2701 ttcgtagcgg agggtgatcc gctccagccg ggtcgaatag cggttgtagg cccacaccgc
     2761 ggggatcgcg gcgaacaggc ccatcgcggt cgcgaccagc gcctcggaaa tgcccggcgc
     2821 gaccgtggcg atcgtggcct gcttcaggga acccaggccc tggaacgcga tcatgatgcc
     2881 ccacacggtg ccgaacaggc cgacatacgt gctgatggag ccgacgttgg cgaggtactc
     2941 gagattgcgt tcgagcttct cgacttcgcg ggtgccggcg acgcgcatcg cacgctcgga
     3001 gctttccagc accgcgcgcc ggtcctcgcc gccgcgctgg cgctggcgca cgaactcccg
     3061 gaatcccgac tcgaacaccg ccgccaggcc accggcctcg ccggcgcggt tgctgacctc
     3121 tcggaacagg gcggcgaggt cgacgcccga ccagaagcgc tcttcgaagc cgtcggcgtc
     3181 gcgggtggac gcgtcgagca tcgccttctt tcggaagatg atcacccacg aggtgaccga
     3241 gaagatcaac aggatcagca acaccagctt gaccggccaa ctggcgtgga tgaccagatc
     3301 gaagatgttg atggaaccgt tcatgcgttt gtcaatcctt gttggagcag gctgattgga
     3361 tcaagaccat gatgcccgcg ggaatcctga cgggcgtgaa agtcgatgcg tcgatgcagg
     3421 cgacgcgcac ctgcgcgcgc gccagcgtaa cgccgtcggc cacgcgcagc acgctttgcg
     3481 cgaagtcgat gctggcggaa cgcacgcgct ccacgtgcac cgtcgcatcc acttcgtcat
     3541 cgagtcgggc cggcttgtca taggcgatgc cgaggtcgcg caccacgaac gcaatgccgt
     3601 gcgcatcccg aagggcttcc tgcccgagtc cggcggaacg catccattcg ctgcgcgcgc
     3661 gttccaggaa acgcaggtat ccggcgtggt acaccacgcc acctgcatcg gtatcttccc
     3721 aatagaccct tatccgccaa ctgaatgccg gctgggcggc gaaaccaggg tcggagtgat
     3781 gtttcccgaa aattcgtgcg agcgactccg acgagcctgg aaacatcact ccgacccctg
     3841 tgtatcgaac aggttcatcg ccggcgcgct gccgcgcggc ggcttcagcc cgaaatgccg
     3901 ccaggccttg gcagtcgcga tgcgaccgcg cgcggtgcgc gcgagcaggc cctgctggat
     3961 caggtagggc tcgaggacat cctcgatggt gtcgcggtcc tcggccagcg ccgccgcgag
     4021 cgactccacg ccgaccgggc cgccttcgaa ctgctccagg accagccgca gcaggcggcg
     4081 gtcgagctcg tcgaagccac cgccgtcgac cctcagcatg tccagcgcag cagacgcggc
     4141 ctcgcgggtg atccgcccgc ccgcgcgcac ctccgcgtaa tcgcgcacgc gtcgcagcaa
     4201 ccggttggcg acgcggggcg tgccgcgcgc tcgccgcgcg atctcgtccg caccttcctc
     4261 gtcgcaggcg atctcgagga tccgcgctgc gcgccgcacg atcgcggcca gctctggcac
     4321 ggtatagaac tccagccggt gcacgatgcc gaagcggtcg cgcaaggggc tggtgagcat
     4381 gcccgcgcgg gtcgtcgcgc ccaccagcgt gaaaggcggc aggtccagct tgatcgaacg
     4441 cgcggccggc ccctcgccca gcatgatgtc gatctggaaa tcctcgagcg ccgggtacag
     4501 cacctcctcg actaccggcg acaggcggtg gatctcgtcg atgaacagca cgtcgcgcgg
     4561 ttgcaggttg gtcagcagcg cggcgaggtc gcccgcacgt tccagcaccg ggcccgaggt
     4621 gctgcgcagg ttgacgccga gctcgttggc gatcacgtgg gagatcgtgg tcttgccgag
     4681 gcccggcggc ccgaacacca gcacgtggtc caacgcatcg ccgcgctttt tcgcggcctc
     4741 gatcgaaagc gacaactgct cgcgcaacgt cgcctggccc acgtactcgg caagcgtgcg
     4801 cgggcggatg ctggcgtcca gcacttcctc gtcgcgggtt tcggcggcgt cgacgacgcg
     4861 cgattcactc atgcggcaat tatgccggaa cgcgcacggc gacggtcgcc gcagcggcag
     4921 tgctccgcca ccactcagcg gctgcgattc gcccgcgtgc gggccgcctt cttcgcggcg
     4981 gccgaacgtt cgctggcacc tttggtgcgc gcggccttct tcgcggctgc cgagcgttcg
     5041 ccggcgctgc gcttgcgcgc ggccttgcgt gcgtgcctgg cgacagcctt cttcgatgcg
     5101 gcggaatggc cttcgcgttt cagcgccttc ctggtcgcac gcgagcgctt ggccgaaacc
     5161 ttgcgcgatg tacccttgtt ggctgccttg cggtcacgct cggcctgctt gatcgtcttc
     5221 tccgaagcct tgcccttctt cggaggcggc aaatccacgc ccgcgcgccg agcctcggac
     5281 aggccgatcg cgatcgcctg ctcgggcgag cgggcgccgt gcttgccttc gcgtatgtgc
     5341 tcgaactcct cgcgcacgaa ctcgccagcc tgggtgctgg cggacttgcc ctcgcgcttg
     5401 tccttcttcg cccgctcgat cgtcttcttt tcgggcatgt cacacctcct tgtcgatgcc
     5461 gtcaaacgct gaagcgcttc ccggcatggt gcgcctgcag tcgccgaatg tcacgccagg
     5521 tttgccgggc ggctcgccga acggggatcc gtcttcgccg ggcgttgcgg gggcgaatca
     5581 gatctccacc tgggccccga gctcgataag gcggttgccg gggatgctga agaacgcggt
     5641 cgcgggcagc gcgttgcggg ccatgaacgc gaacagcttg tcccgccaca gcgccatgcc
     5701 ggcgcggtcg gcggcgacga tcgtctcgcg cgagaggaag aacgtggtgt ccatcatctt
     5761 gatgtcgagc ccgcgcctgg cgcactggtg cagcgcggcg ggcacgtccg gatcatcggc
     5821 gaaaccgaag cgcaaggtca gtttctggaa atcatgcccg aggtccgtca gctcgatcct
     5881 ttcgtccggg tcggcatagg gcgtttccag cgtctccacg ttgaggatca cgttgcacgt
     5941 gtgcagcacc ttgttgtgct tgaggttgtg cagcagagcg tgcggcaccg cgtccgggtc
     6001 gctgaccagg aagatcgcgg ttccctccac ccgggtcggt gggtgctcgg cgagattggc
     6061 gatgaacggc gccagcgcga ggccgctgtg cttcaactgg cgcagcacca gttcgcggcc
     6121 gcgccgccag gtggtcagga tggcgaacac cgcgagtccc agcaccagcg ggaaccagcc
     6181 gccgtcctgg atcttgagcg tattggcgcc gaagaaagac aggtcgatgg tgaggaagaa
     6241 cacgagaacc gcgaggacca gcaagggatg ccacttccac atccgccagg cgacgatcac
     6301 gaacagcacc gtgtcgatgg ccatggtgcc ggtcaccgcg atgccgtagg cgccggccag
     6361 cgcctgggat gaaccgaagc cgagtaccac cagcaccacc aggaccatca gcaaccggtt
     6421 gacccaaggc aggtagatct gcccgacttc ttcgttggag gtgtgcacga tcggcatgcg
     6481 cggcaggaaa ccgagctgca ccgcctggcg catgatcgag aacgacccgg cgatcaccgc
     6541 ttgcgaggcg atcacggcgg cggccgtcgc cagcgcgatc atcggatata gcccccactc
     6601 gggcaccgcg aggtagaacg gattgctggc ggccgccgga ttgtggacca gcaatgcgcc
     6661 ctgcccgaag tagttcagca tcaggcccgg catcacgaat ccgtaccacg cgagcctgat
     6721 gggcttgcgc ccgaagtggc ccatgtcggc gtacagcgcc tcggcaccgg tcacgcacag
     6781 caccaccgcg cccagcacca ggaagccgcc ggcgccgtgg tgcatgaaga aattcacgcc
     6841 gtatgacgga ctgatcgcgc gcagcaccag cgggtgttgc cagatgttgt agaggccgat
     6901 cagccccagc gccacgaacc agatgcacat taccggcccg aacacgagcc cgaccttgcc
     6961 ggtaccgcgc ttctggacca ggaacaccgc caccagcacg atcacggcga tccacaccac
     7021 ccagtgcgaa agcgccggcg ccgccacctc gaggccctcg accgccgaca gcaccgtgat
     7081 cgacggggtg atcacgccat cgccgaagaa cagcgccgcc ccgagtaccg cggccagcac
     7141 gaccagcttg cgggtgcggg gatactggcg cgtggcgcgc tgcgccagcg ccatcatcgc
     7201 catgatgccg ccctcgccct tgttgtcggc gcgcatgatg aaggtgacgt atttcaacgc
     7261 caccacgatc agcagcgtcc acagcaccag cgacagcacg ccgagcacgt tgaacgtggt
     7321 gaccggcatc ccgtgctcgt agccgaacgc ctcgctcatc gtgtacagcg gactggtccc
     7381 gaggtcgccg aacacgatgc cgatcgcgcc caccaccatc acgcgcaggg acttcttccc
     7441 ggcgtcgttg ctcggcgcgg cggcggcgtc ctggtcggtc atcggcccaa tgccgccttc
     7501 agcgccttgc ggatgatggt ttcggcgtcg ccgccttcgg cagcggcgtt cgccgccagg
     7561 cgcgcggctt cggcgggctt gtagccgagt tgctgcagcg cggcttccgc ttccccccgg
     7621 gcgtcgcgcg ggtgcggcgc gctttccggc gataccgcgg tgagcgcgcc ggcgacgcgg
     7681 tcgcgcagct cgaccacgat gcgttcggcc gtcttcttgc cgatgcccgg cacgcgggtc
     7741 agggcgccga cgtcacccgc ctgcaccagc cgcgcgaatt cgttcgtcga aacccccgag
     7801 agcaccgcca gcgagattcg cgcgccgatg ccggacacct tctgcaggtc gcggaacagg
     7861 atccgctcgt cttcgcgcag gaaaccgtaa agcgcgaccg tgtcctcctt gtgggcgtaa
     7921 tgggtcagca gcaccacttc actcccgacg tccggcaggt cgtacaacgt cgacatcggc
     7981 acttccattt catacccgac gccgccgact tccaccagca cccatggcgg gcgtttggcg
     8041 atcagcgtcc cgcgcaggcg gccgatcatg gaccaactcc cacagccggg gaccggggac
     8101 cggggaccgg ggaccagaac ctagtgggat tcatcggcgt cgtccccaag ccgccgccgc
     8161 gatgccggtg cgcgcttcgc tggcacgcag gtgcgcgtgg gtgatcgcga tcgccagtgc
     8221 atcggcggcg tcggcctgca gcggcccgcg catgttcaaa agaatcccga ccatgtgctg
     8281 cacctgctcc ttgccggcgt ggcccttgcc gaccaccgcc tgcttgatcg cgttcggcgc
     8341 atattcgtgg atgacgaggc cgcgcccgac cgcggcgcag atcgcggcgc cgcgcgcctg
     8401 cccaagcttc aaggcggaat ccgcattctt ggccatgaac acgcgttcca ccgccgcctc
     8461 cacgggcgcc tcggcgtcga tgatcgcgcc gagctcgtcg tagatccgct tcaggcgcaa
     8521 cggaaaggtc tcctcacccg ccacccgcaa cgtcgcgtac acgacgcagc gcagcctgcc
     8581 cgtggcatcc gcctcgatga taccgacgcc ggtgcgctgg ctgccgggat cgatgccgag
     8641 gatgcgcacc gagtggggaa cagggaacgg ggaacgggga acggatgcgg gcacggctgt
     8701 tcctctcgag cggctcaatc aggagcggcc gcacgaaaca taccgataca ccggccggac
     8761 ttgcagttcc ctgcaccctg ttccctgttc cctgttcccc gcttcattgc ggcaaatccg
     8821 cgttggaatg cacggcctgg acatcgtcga gttcttccag ccgcgccagc aggccctgca
     8881 ccgcctcgac ctggtcgtcc gccaccggca cgtcgacttc cgcgcgcatg gtcacctcgg
     8941 cgtggtcggg cttgagccca gccttttcca tcgactcgcg gaccgcttcg aaggactccg
     9001 gcgcggtggt cacgtcgatc tggccgtcct cgggccacac cttgatgtct tcggcgccgg
     9061 ccatgatcgc ggcgtcggtg agtttctcct cgtccgcccc gccggcgtag gacaacacgc
     9121 cgatcttgcg gaacaggtac gcgaccgacc cgtcggtgcc gaggttgccg ccgaacttgg
     9181 tgaacgcgtg gcgcacgtcc gaaatagtgc gctggcggtt gtcggtcagg cattcgacca
     9241 ataccgcgac gccgcccggg gcgtagcctt cgaaatgcag ctcctcgtag gtgacgccct
     9301 ccaattcacc ggtggccttc ttgatcgcgc gctcgatgtt gtccttgggc atcgaggccg
     9361 cgatggcctt gtccactgcg gtgcgcaact gcgcattgcc gttgacgtcg ccgccaccgg
     9421 cgcgcgcggc gaccatgatt tcgcgcacca gcttggtgaa cacctggccg cgcttggcat
     9481 cctgcgcgtt cttgcggccg gcgatggaag gacctctacc catggggcgt actcttgata
     9541 ctgaaaagtt gaaagtctag ggaaacactt acggccccgc tacgcttccg cgccaagccc
     9601 catggatcgc cttcctccac aacccccacc gaaccgctct gcggttcggt cgccccttga
     9661 ctcaaggggg caaaaagcac ttccgtaggc cggatggagc gcagcggaat acggggaatc
     9721 cagaaaaaag tacccccggc gctggtcgcc gacccccttc ctgcggaggc gggagtgatt
     9781 gcgcgcggtt ctcgcggctc tctagtccct gcttcctgtt tcccgcacct gcacgtgcag
     9841 ctcggcgagc tgtttttcgt cgatggtgct gggcgcgtcg gtcatcaggt cctgggcgct
     9901 ggttgtcttg gggaatgcga tgacgtcgcg gatcgcctcg gtgccgccga ccagcatcgc
     9961 caggcggtcg atcccgaagg cgatgccgcc gtgcggcggc gcgccgaact tcagcgcgtc
    10021 cagcaggaat ccgaacttgg cgcgggcttc gcgctcgtcg atgcccagga gatcgaagac
    10081 cgcgctctgc atctcggggc ggtggatgcg aatggacccg ccgccgacct cggcgccgtt
    10141 cagcaccatg tcgtagccgc ggctgaccgc gcgcgcggga tcggctttga gcgcctcgat
    10201 gtcatcgacc ttgggcgcgg tgaacgggtg gtggagcgcg acgtggcgcg cccgttcgcc
    10261 gtcgtattcg aacatcggga agtcggtgac ccacagcggc ttccagccgt ccgcgacca
//
LOCUS       DASSNH010000325         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rhodanobacteraceae bacterium isolate SMAG_U1220
            k141_558673, whole genome shotgun sequence.
ACCESSION   DASSNH010000325 DASSNH010000000
VERSION     DASSNH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963375
            Sequence Read Archive: SRR25158181
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodanobacteraceae bacterium (soil metagenome)
  ORGANISM  Rhodanobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:52:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,483
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,423
            CDSs (with protein)               :: 2,423
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Rhodanobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558673"
                     /isolate="SMAG_U1220"
                     /isolation_source="soil"
                     /db_xref="taxon:2052182"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.76805556 N 116.0538889 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFK00_12375"
     CDS             complement(<1..515)
                     /locus_tag="VFK00_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET7593785.1"
                     /translation="MNRVSARDLFEDIKERMALRWVAGRGGAQRALQSGGDGQRRPSE
                     IGYLNIIYPNKLQIVGSEELNYLDGLDSRQRWETVQKIFAQQPVALIVTKDQAIPADL
                     RDAADETGTPLWVSARRGHDVLTFLQYKLARALAPQITLHGVFMEVHSIGVLITGTPG
                     SGKSELALELIT"
     gene            complement(757..1077)
                     /gene="raiA"
                     /locus_tag="VFK00_12380"
     CDS             complement(757..1077)
                     /gene="raiA"
                     /locus_tag="VFK00_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7593786.1"
                     /translation="MQVQVSGHQIEITPALRDYVDARRERLTRRFDHLTSLNVVLEVE
                     KGRQAHRAEGTLAASGAVLHADAVDGDMYAAIDALFDKLDAQARKYKDKLRTHGRDDI
                     RDLA"
     gene            complement(1286..2725)
                     /locus_tag="VFK00_12385"
     CDS             complement(1286..2725)
                     /locus_tag="VFK00_12385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET7593787.1"
                     /translation="MKPGLQLRQSQTLSLTPQLQQAIRLLQLSQLELQAELRELAEAN
                     PLLELDGMDGAPDGDAGERTDSASTEGGSDGEAPVEGGNDADYFDEHSPQASDWTPGS
                     RGFDDDDSAEQQDAAPEGLSEHLLWQANLSGFSAREQAIATAIIDALDEDGYLREGIE
                     PVLAALHALQPAADKDEVEATRRHVQRFDPTGVASLDLADCLAVQLEQLDPATEGREL
                     AREIVAGELELLARRDFAKLARRLNVSEDDVIGAATLIRALNPRPGSAFDPTPAEYVA
                     PDAYAVKTGGRWRVSLSPDCQPRLAINQHYCSLIARAQRSDATWMRGQLQEARWLIKS
                     LEARADTLFKVASAIVRTQSAFLDFGPEAMKPLVMRELAEELGMHESTISRVTTRKYL
                     HTPRGTFEFKHFFSSSVATEDGGSASATAIQAMIRKLVDEEDSRKPLSDQTLTAELNQ
                     RGIRVARRTVAKYREQMRIPSSGERLRAQ"
     gene            complement(3050..3769)
                     /gene="lptB"
                     /locus_tag="VFK00_12390"
     CDS             complement(3050..3769)
                     /gene="lptB"
                     /locus_tag="VFK00_12390"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET7593788.1"
                     /translation="MLTAQGLQKSFRSRQVVQDFGFEIKQGEVVGLLGPNGAGKTTCF
                     YMVVGLIEPDGGRITLDGEDITGLPMHQRARKGIGYLPQEASVFRRLSVADNVLAVLE
                     LRTDLAAAQRRGELEGLLDELKIGHIAGQKGISLSGGERRRVEIARALAANPRFMLLD
                     EPFAGVDPISVGEIQRIVRHLRDRGIGVLITDHNVREALGICEHAYILNEGKVLARGT
                     PAEILAHKQVREVYLGHEFRM"
     gene            complement(3773..4486)
                     /gene="lptA"
                     /locus_tag="VFK00_12395"
     CDS             complement(3773..4486)
                     /gene="lptA"
                     /locus_tag="VFK00_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HET7593789.1"
                     /translation="MPRFRHVRTRIDLLILLAAALGAAGVAHAKQSDKDQPLNLNAVR
                     LVGSQQNGKLVVSGNVTMDQGTFHADGDQATGYSDPADTSQWQRVVLTGRPAHFRQTQ
                     DNGTLVHGQAGTIDYKVSENKVILIGDATVTQEGRGTFHGAKLTYDTDTGAIEGNAEA
                     GSRVHITLQPRKPAPAAGKPASSGTAAPATTSIPAATVAPTATSGAAAAPAPAGTSIA
                     PQPSSTAAMPPSASSAGTH"
     gene            complement(4452..5030)
                     /gene="lptC"
                     /locus_tag="VFK00_12400"
     CDS             complement(4452..5030)
                     /gene="lptC"
                     /locus_tag="VFK00_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET7593790.1"
                     /translation="MKDRRTLTWLIALAIAAAVTQLLVWWLRPPPKPVAMVGPPRSSY
                     QLDHFTLRVFGDEGKLSFTMTAPHLARREGNDSLYINAPEYSFVASDGSDWHGTSQYA
                     WISSDNDLVKLIGKVDLHRPATPKVPKAEIHTADATVWTNEKRMASAAPSVIQEPGSI
                     LRGVGMKADFDTRSLELLSDVHATLSPRSHAH"
     gene            complement(5027..5557)
                     /locus_tag="VFK00_12405"
     CDS             complement(5027..5557)
                     /locus_tag="VFK00_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7593791.1"
                     /translation="MIEPADIDPDLLKRAAKVRLVAFDVDGTLTDGRLLLAEDGRELK
                     AFHVHDGLGIKRLREHGIEVAIISTRISRAVDLRADELGIDHVYQGKADKLACLKEIL
                     AALDVAQDCAAFVGDDVSDLPPMRACGLAVAVADARPEVAREAHWQTRAEGGMGAARD
                     VCDLILAARTQAQAGA"
     gene            complement(5742..6758)
                     /locus_tag="VFK00_12410"
     CDS             complement(5742..6758)
                     /locus_tag="VFK00_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET7593792.1"
                     /translation="MNARTAEPSMDVVPALARTALVMSARKVIRIESGAIADLEQRID
                     GDFSKACRLILACQGRVVVSGMGKSGHIARKIAATLASTGTPSFFVHPGEASHGDIGM
                     ITANDVVLLLSNSGETEEVLTILPFLKRQGNPLIAMTGNARSRLARDADAHLDASVTN
                     EACPLGLAPTASTTAALVLGDALAIALLEARGFTADDFAHSHPAGALGRRLLKIADVM
                     HTGDAIPSVPIEATMTAALMEMSRKGLGMTAVVDGDEILQGVFTDGDLRRTLDDHGVD
                     LRTAPVSQAMSHHPKVITADKLAAEAAQLMETHKIHALLVVDAEQHVVGALNIHDLLR
                     ARVV"
     gene            6902..>7108
                     /locus_tag="VFK00_12415"
     CDS             6902..>7108
                     /locus_tag="VFK00_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016905061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET7593793.1"
                     /translation="MQSSQLKSLLETAFPGARVEVASPDDVHFQSIIVSDAFSGKATL
                     ARHRMVYAALGDRMGGEVHALSLRT"
BASE COUNT         1023 a         2286 c         2463 g         1336 t
ORIGIN      
        1 gtgatcaatt ccagcgccag ttcgctcttg ccgctgcccg gcgtgccggt gatcagcacg
       61 ccgatcgaat gcacttccat gaacacgccg tgcagggtga tttgcggggc cagcgcgcgc
      121 gccagcttgt attgcaggaa ggtgagcacg tcatggccgc ggcgtgcgct gacccatagc
      181 ggggtaccgg tttcatcggc ggcatcgcgc aggtccgccg ggatcgcctg gtccttggtg
      241 acgatcagtg cgaccggctg ctgggcaaag atcttctgga ccgtttccca gcgctgacgg
      301 gagtccagcc cgtccaggta gttcagttcc tcgctgccga cgatctgcag cttgttggga
      361 tagatgatgt tgaggtagcc gatttccgag gggcgtcgct ggccgtcgcc gccggactgc
      421 agtgcgcgct gggcgccgcc ccggcccgcc acccagcgga gggccatgcg ttccttgatg
      481 tcttcgaaaa gatcgcgcgc gctgactcgg ttcactgcat tccccacccg tcgcggcgac
      541 ggatggccga cattctgcca gagtttttta tgcgatcgtc cgtgatcgcg gcgtcggcag
      601 atgcccgtgc agttttgacc ttcgcagcct gcggaccaga acggccatcc acggcctgac
      661 ttttcacaaa agccgctccg caagaggcct ccggaacgcg caggctggat caagccgcgg
      721 cagcggcgga tccggtggcg cgcgccttga cccgccctac gccagatcgc ggatatcgtc
      781 gcgcccgtgc gtgcgcagct tgtccttgta cttgcgcgcc tgcgcatcga gcttgtcgaa
      841 cagcgcgtcg atcgcagcat acatgtcgcc gtccacggca tcggcgtgga gcaccgcccc
      901 ggatgcggcc agcgtgcctt cggcgcggtg cgcctggcgg cccttctcca cttccagcac
      961 cacgttgagc gaagtcaggt gatcgaatcg tcgcgtgagc cgctcgcggc gggcatcgac
     1021 atagtcgcgc aatgccgggg tgatctcgat ctggtggcca ctgacctgga cttgcatggc
     1081 ttgctccttt gcatcacgtg gtagcgggat cccgggctct ccccgggccc gcgcttcgag
     1141 cgcgggtagt gaattttcct ggctgttctc cttggatcgt cgggggtggc ggaaagttgc
     1201 atccagcatg gtggatgcgg tgtgaacctt tcgtttcccg cgcgcgtggg gtgacgcggc
     1261 tcgctcggcg cgttgcagga aggcgctatt gcgcgcggag ccgttcgccg gaactcggga
     1321 tgcgcatctg ctcgcggtac ttggccaccg tgcggcgcgc gacccggatg ccgcgctggt
     1381 tcagctcggc ggtcagggtc tggtccgaca acggcttgcg cgaatcttct tcatcgacga
     1441 gcttgcggat catcgcctgg atcgcggtgg ccgatgcgct gccgccgtcc tcggtggcga
     1501 cgctcgagga aaagaagtgc ttgaactcga aggtgccgcg cggcgtgtgc aggtacttgc
     1561 gggttgtgac gcgcgagatc gtggattcgt gcatgccgag ttcttcggcc agttcgcgca
     1621 tcaccagcgg cttcatggcc tcggggccga agtcgaggaa tgcgctctgg gtgcgcacga
     1681 tcgccgaagc gaccttgaac agcgtgtcgg cgcgcgcctc gaggctcttg atcagccagc
     1741 gggcctcctg cagttgcccg cgcatccagg tcgcgtcgct gcgttgcgcg cgcgcgatca
     1801 ggctgcagta gtgctggttg atcgcgagcc ggggctggca atcgggtgac agtgacaccc
     1861 gccagcgtcc gccggtcttg accgcgtagg cgtccggcgc tacgtactcg gctggggtcg
     1921 ggtcgaacgc cgatcccggg cgcggattta gcgcgcggat cagggtggcg gcaccgatga
     1981 cgtcgtcctc ggagacgttg agccggcgtg ccagcttggc gaagtcgcgg cgggcgagga
     2041 gctctagttc gccggcgacg atttcccgcg cgagttcgcg gccttcggtc gcaggatcca
     2101 gttgctccag ttgcacagcg aggcagtcgg ccaggtcgag gctcgcgacg ccggtcggat
     2161 cgaagcgttg cacgtgccgg cgcgtcgctt cgacttcgtc cttgtcggcc gccggctgca
     2221 acgcgtgcag ggctgccagc acgggttcga tgccctcgcg gaggtaaccg tcctcgtcga
     2281 gcgcgtcgat gatggcggtg gcgatggcct gctcgcgcgc cgaaaagccg ctgaggttgg
     2341 cttgccacag gaggtgctcg gacaggcctt cgggcgcggc atcctgctgt tcggccgagt
     2401 catcgtcgtc gaagccgcgg ctgcccgggg tccagtcgga tgcctgcggc gaatgttcgt
     2461 caaagtaatc ggcgtcgttg ccaccttcga cgggagcctc gccgtcggaa ccgccctccg
     2521 tggacgcgga gtcggtgcgc tcgccggcgt cgccatccgg cgcgccgtcc attccgtcca
     2581 gttcgagcag cgggttggct tccgcgagtt cgcgcaattc ggcctgcaat tcgagctgcg
     2641 acaactgcaa aagccttatc gcctgctgga gttgcggtgt cagcgacagc gtctgcgatt
     2701 ggcgaagctg gagaccgggt ttcatggtgg cgttcgtgga tggccgccgg catcgtatac
     2761 cgtgggaggc cgcaagggta gtgcaagggc acgttttatg cattatttgt gcgatcgtcc
     2821 gtgatcgcgg tctcgctcga caccctgcga ctgtttgttt tgcgatcatc cgtgatcgcg
     2881 gcctcgatca aggtcgccgc aatcagaacg cgccactgcc ggtggagacc agaacggcca
     2941 tccttggcct gacttttcac aacagccgct tcctacgggg gcggatcaca acagccgctt
     3001 cgtacgaggc ggacttgtgc cgcgcttcaa agctggttcg cgacagtggt cacatcctga
     3061 attcgtggcc gaggtagacc tcgcgcacct gcttgtgggc gaggatttcc gcgggcgttc
     3121 cgcgcgcgag caccttgcct tcgttgagga tgtaggcgtg ctcgcagatg ccgagcgctt
     3181 cgcggacgtt gtggtcggtg atcagcacgc cgatgccgcg gtcgcgcagg tgccgcacga
     3241 tccgctggat ttcgcccacc gagatcgggt cgacgcccgc gaacggttcg tcgagcagca
     3301 tgaagcgcgg attggctgcc agcgcgcgcg cgatctcgac acgccgccgc tcgccgccgg
     3361 acaggctgat gcctttctgg ccggcaatat gaccgatctt caattcgtcc agcagtcctt
     3421 ccaattcgcc gcgacgctgc gcggccgcca ggtccgtgcg cagctcgagc accgccagca
     3481 cgttgtcggc gaccgacaac cggcggaaca ccgaagcttc ctggggcagg tagccgatgc
     3541 ccttgcgcgc gcgctggtgc atcggtagcc cggtgatgtc ctcgccatcc agcgtgatgc
     3601 gcccgccatc gggctcgatc agcccgacca ccatgtagaa gcaggtggtc ttgccggcgc
     3661 cgttgggccc gagcaggccg acgacctcgc cctgcttgat ctcgaacccg aaatcctgca
     3721 caacctgccg ggagcgaaag cttttttgca gcccctgcgc ggtcagcatg gatcagtggg
     3781 ttccggcgga cgaggcggaa ggcggcattg ccgccgttga cgaaggctgc ggcgcgatgg
     3841 atgtgccggc gggcgcgggt gccgcggctg cacccgaagt cgcggtcggg gcaaccgtcg
     3901 cggcaggaat cgaagttgtg gccggggcgg ccgtaccgga tgatgccggc ttgccggccg
     3961 ccggcgcagg cttgcgtggc tgcagcgtga tgtgcacgcg gcttcccgcc tcggcgttgc
     4021 cttcgatcgc gccggtgtcg gtgtcgtagg tgagcttggc gccgtggaaa gtgccgcgcc
     4081 cttcctgcgt gacggtcgcg tcgccgatca ggatcacctt gttttcggaa accttgtagt
     4141 cgatggtgcc ggcttggccg tgtaccagcg tgccgttgtc ctgcgtctgg cgaaagtgcg
     4201 cggggcgtcc ggtcagcacc acgcgttgcc attggctggt gtcggcgggg tcgctgtatc
     4261 cggtggcctg gtcgccgtcg gcgtggaagg ttccctggtc catcgtgacg ttgccgctca
     4321 ccaccagctt gccgttctgc tggctaccca ccaggcgcac ggcgttcaag ttgagtggct
     4381 ggtccttgtc cgactgcttc gcgtgcgcga cgccggccgc gcccagcgcc gcggcgagca
     4441 ggatcagcag gtcaatgcgc gtgcgaacgt ggcgaaagcg tggcatggac atcggagagc
     4501 aactccaggc tgcgggtgtc gaagtcggcc ttcattccga cgccgcgcag tatagagccc
     4561 ggctcctgaa ttacgctggg cgcggcgctg gccatgcgct tttcgttggt ccagacggtc
     4621 gcgtcggctg tgtggatttc cgccttcgga accttcggcg tggccgggcg gtgcaggtcc
     4681 accttgccga tcagcttcac gaggtcattg tccgaactga tccacgcgta ttgcgaggtg
     4741 ccatgccagt ccgagccatc gctggcgacg aaggaatatt cgggcgcgtt gatatacagg
     4801 gaatcgttgc cttcacgacg cgcgagatgc ggcgcggtca tggtgaacga cagcttcccc
     4861 tcgtcgccga agacgcgcag ggtgaagtgg tccagctggt aactggatcg cggcggcccc
     4921 accatcgcga cgggcttcgg cggcggccgc agccaccaga ccaggagctg cgtgaccgcg
     4981 gcggcgatcg cgagcgcgat cagccacgtg agggtgcggc gatccttcat gccccggctt
     5041 gcgcctgcgt ccgagccgcg aggatcaggt cgcacacgtc gcgggcggca cccatgccgc
     5101 cttcggcacg cgtctgccag tgcgcttcgc gcgcaacttc cgggcgcgcg tcggcaacgg
     5161 ccaccgcgag gccgcaggcg cgcatcggcg gcaggtccga cacatcgtcg cccacgaagg
     5221 ccgcgcagtc ctgggcaacg tccagcgcgg cgaggatttc cttcaggcag gcgagcttgt
     5281 cggccttgcc ctggtagacg tggtcgatcc ccagctcatc cgcgcgcagg tccacggctc
     5341 ggctgatccg ggtgctgatg atcgccactt cgataccgtg ctcgcgcagc cgcttgatgc
     5401 cgaggccatc gtgcacgtgg aaggccttga gctcgcggcc gtcctcggcc agcagcagcc
     5461 gcccgtcggt gagtgtgccg tcgacgtcga acgcgaccag ccggaccttg gcggcgcgct
     5521 tcaacaagtc ggggtcaatg tcggccggtt cgatcatctg tgggcaggcg cgtcaagggt
     5581 gctttcttcc cccttcgctc gcgaagggcg gaacgaagtg aacgcgcgcg aagcgcaaga
     5641 gggggatggc cttttgccgc cggcacatcc ccccgccgct tcgcggcgtc ccccttcgtc
     5701 ctgaaggggg taacaactcg tatgcttccc gaatcccgaa ttcaaaccac ccgcgcgcgc
     5761 aacaggtcgt ggatgttcag cgccccgacc acgtgctgct ccgcgtccac caccaacaag
     5821 gcgtggatct tgtgggtctc catcaactgc gcggcttcgg cggccagctt gtcggcggtg
     5881 atgaccttgg ggtggtggct catggcctgg ctgaccggcg ccgtgcgcag gtccacgccg
     5941 tggtcgtcga gggtgcggcg caagtcgccg tcggtgaata cgccctgcag gatttcgtca
     6001 ccgtcgacca ccgcggtcat gccgagcccc ttgcgcgaca tttccatcag cgccgcggtc
     6061 atcgtggctt cgatcggcac gctcgggatc gcgtcgcccg tgtgcatgac gtcggcgatc
     6121 ttgagcaggc gccggccgag ggcgccggcc gggtgcgaat gggcgaagtc gtcggcggta
     6181 aagccgcgcg cctcgagcag cgcgatcgcg agcgcatcgc ccagcaccag cgccgcggtg
     6241 gtactggccg tcggggccag tccgagcggg caggcttcgt tggtaacgct ggcgtcgagg
     6301 tgggcatcgg cgtcgcgcgc gagccgtgag cgcgcattgc cggtcatcgc gatcagcgga
     6361 ttgccctggc gcttgaggaa cggcaggatc gtgaggactt cctcggtttc gccggaattc
     6421 gaaagcagca gcaccacgtc gttcgcggtg atcatgccga tgtcgccgtg gctggcttcg
     6481 cccggatgca cgaagaacga cggggtacca gtggacgcga gggtcgcggc gatcttgcgc
     6541 gcgatgtggc cggacttgcc catgccggac accaccacgc gtccctggca ggcgaggatc
     6601 agccggcagg ccttgctgaa gtcgccgtcg atgcgctgct cgaggtcggc gatcgccccc
     6661 gactcgatgc ggatcacctt gcgcgcgctc atcaccagcg ccgtgcgggc cagcgccggg
     6721 acaacgtcca tggaaggttc ggcggtgcgc gcgttcatgc gggagtgctc ctgtacgtgc
     6781 gtgtcttgca ttcgggccgt gcgatttccg ctaccatcgc gggctgtcat tgtataggcg
     6841 atggacgggt tgcccgcgtt tcccctgttt tggaggcggg ttgcccgttc gagtgggatt
     6901 catgcagtcc agtcaattga agtccctgct ggaaacagcc ttccccggcg cccgcgtcga
     6961 ggtggcaagc ccggacgacg tccatttcca gtcgatcatc gtatcggatg cgttctccgg
     7021 caaggccacc ctcgcccggc accggatggt gtacgcggcg ctcggcgatc gcatgggcgg
     7081 ggaggtgcac gcgctgtcgc tgcgcacc
//
